LOCUS       DAOMXO010000001        16407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_1995_length_16407_cov_5.501835, whole genome shotgun sequence.
ACCESSION   DAOMXO010000001 DAOMXO010000000
VERSION     DAOMXO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 16407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16407
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1995_length_16407_cov_5.501835"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLH57_00005"
     CDS             complement(<1..654)
                     /locus_tag="PLH57_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01018.1"
                     /translation="MFDRLIFGTVALFTVMVYGPMATMSISAVAVMLLATIVVVREPK
                     VLAKLQTARKLFYVSLALAVACVWSLVWAKWTDLEFMGAHPNVQILKDSAKAWHLFFP
                     FFLLVLLSRLSDRKFRTIIVIWLFAGLLISFFAFAQHYFPIYKPSKLAYLNYPNYVRG
                     TGFWDRLSGSYHATGFTGFHLSFASIMAFPFGVWLTLAVLSLRRGRHNVWSLGAFSVP
                     "
     gene            complement(647..2005)
                     /locus_tag="PLH57_00010"
     CDS             complement(647..2005)
                     /locus_tag="PLH57_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01019.1"
                     /translation="MAICNDTMKLWRSLTRIEIASLCLRILIWAFVASTGHWVAGDSL
                     GFMGHSVRFCAGDFFGGDSTKSPLYTLFACIGSLPTFPFVGPLVWKGVGFAAAIFFQT
                     VLVWAAGVYVRKSLFAQSREGATKFWQVAYHFDPVLLVYSSLLMSDALFAALLLIATL
                     QIARYFHGSRWTPLAAAATGAIFAFIVLQRSLGMVWVAWTIGFVFIFYGRSLWVRLRR
                     GQKMLERPASVAAMFFLVFGICLAPRIVYQRVTQNTFSPHVQGQTWLPSVAAAVAASE
                     QRGLSFKEAEDLWNREHPNATWIDALETFKKYPASFAKLVVKGLARTTIGHVNVEIGL
                     LLTGKTVFGPAWFKNQEKFTGEPGGAIYLQEKGLSAKIFWLLGILAVVIWMGWVYFFA
                     LRSIIFFRTSVGAWVLAWASGVVVIMCSLPLIFGDARFRLGYFPVLLVLWGLSLRGKK
                     LV"
     gene            2036..3220
                     /locus_tag="PLH57_00015"
     CDS             2036..3220
                     /locus_tag="PLH57_00015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK01020.1"
                     /translation="MPNNKASLLIIFLVVFIDLIGFGIVIPILPYYAHSFGASGETLG
                     WLMASYSAAQFLCAPFWGRLSDHFGRRPVLIATMIGSAASLALTASASSLTQIFIARI
                     LAGLFGANISTASAYIADVTSEADRAKGMGLIGAAFGIGFVVGPAVGGILSRWGYATP
                     FLFASGLACLNTLFAIWKLREPPTTAGVREINRLARRISRERLRTAFGDARTRLAIVV
                     FFLGTVAIAQLEVTFALYMKAMHGFNAEKAGWYLAFMGLAMAVIQGGLIGKLSRKFGE
                     RRLIICGTFIMAGAMVWFASLQTAGIWAFAALGLLAVGNGITNPSLSSLASKGALKNQ
                     LGATMGVYQSAGSLARILAPPMAGVLYDRATIQSPFYCAAILMAIACVGVFFGSKQPM
                     GR"
     gene            3230..3505
                     /locus_tag="PLH57_00020"
     CDS             3230..3505
                     /locus_tag="PLH57_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HRK01021.1"
                     /translation="MNTTNEQSSTSSMDPQIYSPKSYMVTLILCFIFGGFGIHRFYVG
                     KVGTGILMLLTLGGLGIWALIDLVLVVSGSFKDSENRAIVHPKTIAR"
     gene            complement(3515..4603)
                     /locus_tag="PLH57_00025"
     CDS             complement(3515..4603)
                     /locus_tag="PLH57_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRK01022.1"
                     /translation="MSSHDNHKHSHHVTPLKVYYSVWIGLLVLTVVTVGSSYIDFGGV
                     NNILLAMLIATVKAVMVALYFMGLKYEGKENNVTFFGTFLFLLIFITLAGADIFFRTD
                     PTVKARVDASELPAEASNVDVNTIKNSTPELVLRGKTLYAQQCATCHGANGQGDGPAA
                     AALNPKPRNFTEGVWKQGGTPAGIYKTLTNGIPGTPMPAFPGFSVEDRFALAHYIREF
                     DAKAPSADADTAEVLASLGIGAAPKPKLSIAVAMERSEVPSEQSTTEPGAVYHESATD
                     GPGERLYGQMCMSCHGANGQGALAKNVGVQSPLRIATKALTRYSSTQSQGEFVRALTE
                     SNPSGLHGYGALSNAQWNALYEYVRSLR"
     gene            complement(4616..5269)
                     /locus_tag="PLH57_00030"
     CDS             complement(4616..5269)
                     /locus_tag="PLH57_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HRK01023.1"
                     /translation="MSHDASTTGSHHYAHHFESADHEFETSKFGMWVFLVTEIMLFGG
                     LFIAYGIYRGMYPEMFMEAHHALNKIMGAVNTVVLICSSLTMALAVGMAQRNERAKSA
                     KLMMATLFFACMFLVIKYFEYSHKIHIGLLPGAFYNNPDITHPKAPIFFSIYFLMTGL
                     HGLHVLIGMGLIIWVLRRTMKGEFSSAYYTPVEIAGLYWHLVDLIWIYLFPLLYLIG"
     gene            complement(5273..6892)
                     /gene="ctaD"
                     /locus_tag="PLH57_00035"
     CDS             complement(5273..6892)
                     /gene="ctaD"
                     /locus_tag="PLH57_00035"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HRK01024.1"
                     /translation="MAHDASASHGHDVDYLNDGKTVWSWLSTIDHKRIGIMYLVTVLI
                     FFGVAGLLAIGIRLEHWGPTRTIMGPDTYNKVFTLHGAIMVFMFIIPAIPSALGNFLL
                     PLMLGAKDVAFPRLNLFSYYVFLFGATLAVVAILAGQGVDTGWTFYTPYSIKSQTGVI
                     LTVLGAFIMGMSSILTGLNFIATIHKLRAPGMTFHRMPLFVWATYATSIIQVLATPVI
                     GITLLLLVAERVLKVGIFDPALGGDPVLFQHFFWFYSHPAVYIMILPGMGIISEIMPV
                     FSRKPIFGYRAIAYSSLAIAAISFLVWGHHMFVSGQSDFSNIIFSFLTVLVAVPTAIK
                     VFNWLSTMYKGSIKLDVPMLYALAFLFLFTIGGLTGIFLAALSIDVHLHDTYFIVAHF
                     HYVMMGGTVMALFGGLHYWWPKFFGRMYPEKLGRFAWALVFFGFNLLFLPQFYIGGKG
                     MPRRYYNYDPVYTLGHQVSTIGSLMVGFGLMLTLGYLFWAAKKGQKATQNPWGAKTLE
                     WTDAASPPITHNFHGQPTVDNWPYEYKSERV"
     gene            complement(6916..7947)
                     /gene="coxB"
                     /locus_tag="PLH57_00040"
     CDS             complement(6916..7947)
                     /gene="coxB"
                     /locus_tag="PLH57_00040"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRK01025.1"
                     /translation="MFWQSAYASTVGLPQSSTIAPTVDILYDFLVYLSVFFLILVTVV
                     MVYFTVKYHRSKTGRETAYIVGSHTLETIWTVVPLVLMLFVFAWGLKGYREMRAPMKD
                     PVEINIIGRQWLWNIEYTNGRKTLNELVLPKGKPVRLIMTSEDVLHSFFIPNFRIKQD
                     VVPGRFTTLSFIATQPGVHIVFCAEYCGTAHSDMLAKAVVVEPEEFDEWTRTGKLPAH
                     VNLNQIIGAVGAPTSVAAATGNGSSQPSQTPAERGKALAASKGCIACHSDNGTRMVGP
                     SYKGIFGKKEEMADGTMVTVDENYIRKSLMDPNAQIVKGYTPSMPPFAGLLKDEEVND
                     LIAYIKSLK"
     gene            complement(7947..9710)
                     /locus_tag="PLH57_00045"
     CDS             complement(7947..9710)
                     /locus_tag="PLH57_00045"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRK01026.1"
                     /translation="MRSVEQTNSLPGTGLEQTVGGESLVKGKLSFGRKVGAFVQLTKP
                     RIILLFALTGLTAMIVQGTLLSHPLALWMVCLGIALTGGSANAFNQFLDRDIDSVMER
                     TKAKRPLPNGSLSPNAALIFACVSGAVATFLLYTFGGFLAAALGVATIFYYVVVYTMW
                     LKRSTPYNIVIGGAAGASAPMIGWAAATGNISFDAFVMFLVIFMWTPPHFWALALCLK
                     DEYAKVSVPMLPVVAGVEETRRQIFLYTLALVPLTLVPFITGAVGALYFFGAAVLGGI
                     FLKLAWKITKVSAEQLKATAWRTFGFSILYLLALFLFMIADALITPRAHAAEKLPKEL
                     EGIGIEEKFGAQVDLNLTFKNEKGEVVPLKSVVSGKKPVMLLLAYYGCPNLCNYFLNG
                     VTDGFKELQYKIGQEFDVVTVSIDPREDADLAAAKKNSHIESLGKPDAAAGWHFWVND
                     QPRTSPHAVDTNAKKLAEQVGFKYRWEDSTDQYAHSAAIVVLTPEGKVSRYLYGIQFA
                     ARDLRFALLEAGGRKIGTFVDRMILFCYHYDPEAKSYSLYARNVMRAGGGLTVLILAG
                     ILGTVIQRSHRPTRPTTRRNS"
     gene            complement(9688..10689)
                     /locus_tag="PLH57_00050"
     CDS             complement(9688..10689)
                     /locus_tag="PLH57_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HRK01027.1"
                     /translation="MMSVELTQPGFGVGTGKASKDLAPGNRFWTLSFVQVLSKLLVGV
                     TFFLISLGSLVRNEDAGLACPDWPLCHGHVVPPMDYQVFLEWFHRLVAGFVSIGLLTL
                     SVVVLTNRSLRQLVGKWCIAALIMLAAQVVLGGMTVLGLLHPGWVSSHLAVGLAFFAA
                     LVVISIKFSELSGRGRTEPLLAVCFRNRAEESGLSNLAAIALFAIYGQSLMGGGVASN
                     YAGLACPDFPKCLGVWWPPFEGIIRFQMIHRIGAIVVTLVAVTFFVAVWAKKKQLSPS
                     VVGWSVFALVLLGIQIFLGIGNVIWQLPLLMSVAHLSVAALLIGSFVVLFYEIRRTN"
     gene            complement(10701..11435)
                     /locus_tag="PLH57_00055"
     CDS             complement(10701..11435)
                     /locus_tag="PLH57_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01028.1"
                     /translation="MRKNSLAQDVDGDHKHTLANVRTAAVFLPLFGISFSVASFDLLM
                     SLEPHWFSTIYGIYCFAGLVFSGFSLLAILVIYGRRQGVLTDQMVNENHIHDIGKMMF
                     AFTVFWAYIMFSQLMLQWYANLPEETVYYIRRFNGSWWPYFWTLLIVHFAIPFFSLLP
                     RGVKRNEKVLFGMAVFMLLAQWMDVYFLVMPVFFKDGPVFGWIELGTFAAFAGVFGLS
                     VIRFLERVPAVPVKDPRLSQCLGHAQ"
     gene            complement(11454..11906)
                     /locus_tag="PLH57_00060"
     CDS             complement(11454..11906)
                     /locus_tag="PLH57_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01029.1"
                     /translation="MSHHTHTEFQPSSVRSMRFSAGAKSTLLVLAVIGSILTVTALLK
                     QPARAWPNFLVNYFYFLALSLFGAFFVALQHITNAYWSVTVRRFAEGLMSYLPVALVL
                     GVVLMFGTHHLYEWTHTEVVANDHLLHLKAPYLNMKFFVVRMILFFVI"
     gene            complement(11911..12486)
                     /locus_tag="PLH57_00065"
     CDS             complement(11911..12486)
                     /locus_tag="PLH57_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRK01030.1"
                     /translation="MKSAWCERLVLVAALGVFAAGCTKNPKKPAFEFMPHMADSPAVK
                     GQERDSDGNGMGVPPEGTVPQGYQPYAHRGDPDAAKGLRNPLRSTKPVLARGKLMFET
                     YCMVCHGRKGEGDGSIVPKFPRPPTLHSEKVRDWSDGQIFHVISDGQNLMPKYASQIA
                     PEDRWAIVHYVRTLQRSLNPTAEDLKAAGGR"
     gene            complement(12467..12970)
                     /locus_tag="PLH57_00070"
     CDS             complement(12467..12970)
                     /locus_tag="PLH57_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRK01031.1"
                     /translation="MKGIVGIFKDPDQLLHAAEQVRDRKFKRFDAFTPFPVHGLEHAM
                     GLKRSFLPWVTFIAGLTGLAIGTGFQIWTSAFDWPLNVGGKPFISLPAFVPIMFELTI
                     LLAGLATAGVMIITCGLPNLKAKIIDPRLTDDHFALFISSDDPLYREAEISELFKKAG
                     ANEVRMV"
     gene            complement(12967..14361)
                     /gene="nrfD"
                     /locus_tag="PLH57_00075"
     CDS             complement(12967..14361)
                     /gene="nrfD"
                     /locus_tag="PLH57_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRK01032.1"
                     /translation="MSSTEVVLRNAASLAKEPLIDPSKSYADVTNDVCDPVERFPTKL
                     WFKAFMVANIVMFIGLFCMGLTIATGIGQWGLNRTVGWAFDITNFVFWIGIGHAGTLI
                     SAILFLFRQKWRTSINRSAEAMTIFAVMTAAIFPLLHTGRQWLAAFWLFPYPNSRDLW
                     VNFRSPLLWDVFAVSTYFTISLVFWYIGLIPDIASVRDRAKNKIRKTIYTILSLGWTG
                     SARAWNHYETAYMLLAGLSTPLVLSVHSIVSFDFAVSILPGWHTTIFPPYFVAGAIFS
                     GFAMVITLLLIAREVLPQLKSYIRHVHLENMCKVIMLTGLMVGYAYGTEFFVAWYSGN
                     EYERFVFINRAFGPYGWSYWTMVTCNVLIPQLFWSKSIRRNLKAMFVITIFVNIGMWF
                     ERFVIIVTSLHRDFLPSSWSMFTPTWVEIGIMVGSFGLFFTLFLAFCRVLPMIAMAEV
                     KSVIHGAKHEEAHK"
     gene            complement(14358..>16407)
                     /locus_tag="PLH57_00080"
     CDS             complement(14358..>16407)
                     /locus_tag="PLH57_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRK01033.1"
                     /translation="STVSKDLGIDASALKKVANELWADRGKSIVVGAGHGLKGAAGNA
                     LEVVVALLNSALENEGNTVDGTTTPFERRTNFEALTKLIRDMKAGAVDVLVISGVNPA
                     YGLPKNAGFEEALAKVGTVVYVGTKFDETARLSDYVAAASHALEAWGDAEAQRDLFSI
                     QQPTIRPLYNTRSFEETLMAWAKKLGMSGALVTSPTWHEFVKSNWKNTIYSNFSISAP
                     FALFWESLLREGVFDGVAAKGSRELGTSRARSFDTSSLRSVAEWTAKNGGVTSEMTLV
                     LYPKIGMYDGRSANNGWLQELPDPISKITWDNYAALSVATAKKLGVVEGDVLRLKTSS
                     GELHVPAHVQPGVHDQVVALAVGYGRIGSGRVSENVGVNAYILASANAETLDLSGIPV
                     TIEKTSKREKLAATQMHHRLEGRPIVREADYPEYVSNPKAGNPDGHPLVTLWQEHKYP
                     GYRWGMAIDLSSCTGCGACVIGCQSENNIPVVGKKFVETGREMHWIRIDRYYNGSDDN
                     PETVHQPMLCQHCENAPCETVCPVLATMHDDEGLNQQVYNRCVGTRYCANNCPYKVRR
                     FNWFTFTEVAKPLHLAYNPDLTVRTRGIMEKCTFCMQRIRDGKDRAKDLGTTVVDGQV
                     QTACQQSCPSDAIVFGNVNDKNSRIAKINADPRGYHVLEELNVKPSVTYLTKIRNKNA
                     "
BASE COUNT         4654 a         4208 c         3780 g         3765 t
ORIGIN      
        1 gggcactgaa aaagccccga gagaccaaac attgtggcgc ccacgacgta aagacaaaac
       61 ggccagcgtt aaccacacgc caaacggaaa cgccataatc gaggcaaagc tcaaatgaaa
      121 gcccgtgaac ccagtagcat gatacgagcc actgagacga tcccaaaatc cggtaccacg
      181 cacatagttt ggataattta agtaagcgag tttcgagggc ttatagattg gaaaataatg
      241 ttgagcaaat gcgaaaaagg aaatgagaag acccgcgaat agccaaatta caatgatggt
      301 gcgaaattta cgatcgctga gcctcgagag taatacgaga agaaaaaatg gaaaaaacaa
      361 atgccaggct ttcgcagaat cttttaaaat ctgaacgttg ggatgggcac ccataaattc
      421 aagatcagtc catttcgccc aaacgagcga ccaaacacag gccaccgcca aggcaagcga
      481 aacgtaaaat aacttgcgtg cggtctgtaa cttagcgagt accttgggtt ctcgcacgac
      541 cactatagta gccagaagca tgacggcgac agcgctgatg ctcatggtgg ccatcgggcc
      601 ataaaccatg acagtaaaca aagcgacagt tccaaagatt aaacgatcaa acaagcttct
      661 taccccgcag acttagcccc caaaggacta acaacactgg aaagtacccc aaccgaaatc
      721 gtgcatcacc gaagatgagc ggcaacgaac acatgatgac tacgacgcca cttgcccagg
      781 ccaaaaccca agcacctacg gatgttcgaa aaaaaataat acttctcaaa gcaaaaaaat
      841 acacccatcc catccagata acgaccgcaa gtataccgag gagccagaaa attttcgcac
      901 taaggccttt ttcctgcaaa taaatcgcac cgcccggctc gccggtaaac ttttcttggt
      961 ttttaaacca cgccggtccg aacacggttt tgccagtgag aagaagtccg atttctacat
     1021 taacgtgccc tatcgtcgtt cgtgccaaac ctttaacaac aagcttggca aagcttgcgg
     1081 gatacttttt aaaggtttct aatgcgtcga tccaggttgc gtttgggtgt tcgcgattcc
     1141 aaagatcttc agcttcttta aaggacaaac cccgctgttc gctcgctgct accgccgctg
     1201 ccacactggg caaccaagtc tgaccttgaa cgtgaggtga aaaagtattt tgagtcacac
     1261 gctgatagac aatccgggga gcgaggcaga ttccaaagac tagaaaaaac atagctgcca
     1321 cgctagcagg acgctcgagc attttttgac cgcgcctcaa acgaacccaa agagaacgtc
     1381 cataaaatat aaacacaaat ccaatggtcc aagccaccca aaccataccg agtgatcgtt
     1441 gcaaaactat aaaagcaaat atcgcaccgg tcgctgcggc agccaaaggg gtccaacgcg
     1501 atccatgaaa atagcgcgca atttgcaagg ttgcgattaa cagcaacgct gcaaaaagag
     1561 cgtcgctcat aagtaaactg gaatatacca aaagaacggg atcaaaatga taagccacct
     1621 gccaaaactt ggtggcccct tctctcgatt gagcaaacaa actcttgcga acataaactc
     1681 cggcggccca gactaaaaca gtttgaaaaa agatcgcagc ggcaaaacca acccctttcc
     1741 aaaccagtgg acctacgaac ggaaaggtag gcaaagaacc aatacaggca aacagggtgt
     1801 atagtgggga cttggtgcta tctcccccaa aaaaatctcc cgcacaaaac cgaacgctgt
     1861 gccccataaa ccccaacgaa tcgcccgcga cccagtggcc ggtgcttgcg acaaaggccc
     1921 aaataagaat tcgcaggcat aaactcgcaa tttcaattcg ggtaagggat cgccaaagtt
     1981 tcatggtgtc attacaaatt gccaaacaat caacccgctt gtaaagcttg agcaaatgcc
     2041 aaacaacaaa gcttctcttt taattatatt cctagtcgtc tttatcgatc tcataggttt
     2101 tggcatcgtc attccgattc taccttatta cgctcactct tttggcgcgt ctggagaaac
     2161 gttgggctgg ctcatggcct cttactcggc ggcccagttt ctttgtgctc ctttttgggg
     2221 gcggctctcg gaccattttg ggcgtcgccc agttttgatt gctaccatga ttggttcggc
     2281 cgcgtccctt gctttaacgg cgagcgccag ttctctgacc caaattttta ttgcgcgcat
     2341 tctggcagga ctttttggag caaatatttc tacggctagt gcttacattg ccgacgttac
     2401 gagtgaggct gaccgggcta agggcatggg gttgatcggc gcggcctttg gaatcggttt
     2461 tgttgttgga cccgcggtcg ggggtatctt gtcgcgttgg ggttatgcaa caccgttcct
     2521 atttgcttct ggtctcgcct gcctgaacac gttgttcgca atttggaaac tccgagaacc
     2581 gccgacgact gctggagtac gcgaaatcaa tcgtttggcg cggcgtatca gtcgggaacg
     2641 actacgcact gcattcggtg acgcacgcac aagactagct attgttgtgt ttttcttggg
     2701 caccgtcgcc atagcgcagc tagaagtaac ctttgcgctt tatatgaaag ccatgcacgg
     2761 atttaacgcc gaaaaggccg gctggtactt agcttttatg ggactcgcca tggcggtgat
     2821 tcagggagga ttgattggta aactttcgcg aaaatttggc gaacgaagac tgatcatttg
     2881 cggcacattt atcatggctg gagccatggt ttggttcgcc tcgttgcaga cggccggaat
     2941 atgggcgttc gcggcactag gtttattggc agttggcaat ggtatcacca acccttcact
     3001 ttcgagttta gcgtcgaaag gagccttgaa aaatcagctt ggcgccacca tgggagtgta
     3061 tcaatctgcc ggaagcctcg ctcggattct ggctcccccg atggccgggg tactatatga
     3121 tcgagctaca attcagtcgc ccttttattg cgccgctatt ctcatggcaa tcgcctgcgt
     3181 cggagtattt ttcggttcca aacagccaat gggccgctaa agtgtctaca tgaacactac
     3241 aaacgagcaa tcctcgacct cgagcatgga cccgcagatt tatagcccca agtcctatat
     3301 ggtcacgttg atactctgtt ttatattcgg agggtttggt atccaccgtt tttacgtagg
     3361 taaggtcggc actggcattc ttatgctgct aaccttgggc ggacttggaa tatgggcact
     3421 gatcgatttg gttctggtcg tgagtgggag tttcaaagat tcagaaaacc gagcgatcgt
     3481 gcatcctaag acaatcgctc ggtagtcttt tttcttatct taaactacga acgtactcgt
     3541 aaagagcatt ccactgcgcg ttcgacaacg ctccatagcc gtggagaccg ctagggttcg
     3601 attcagtcaa ggcgcgtaca aactcaccct gagattgcgt gctcgaatac cgcgtgagcg
     3661 cttttgtggc gattcgcagc ggtgactgaa ccccgacgtt tttggctaga gcaccttgcc
     3721 cgttcgcacc atggcaactc atgcacatct gtccgtacaa tcgttcacct ggaccatcgg
     3781 tcgcgctttc gtggtaaacg gctcctggtt ccgtggtgct ttgctctgaa ggaacttctg
     3841 agcgttccat agctactgcg atcgagagct taggttttgg cgctgcaccg attccaagtg
     3901 aggccaagac ttctgcggta tcggcgtcgg cggaaggcgc cttggcatca aattctcgaa
     3961 tgtaatgagc aagggcaaaa cggtcttcaa cactaaaccc tggaaaggcc ggcattggtg
     4021 tacccggaat gccgttggtc aaagttttat aaattccggc aggtgttccc ccctgcttcc
     4081 aaacgccttc ggtaaaatta cgaggcttgg ggttcaaagc ggcggcggcg ggaccatccc
     4141 cttggccgtt tgctccgtga cacgtggcac attgttgtgc gtaaagtgtt tttcctctga
     4201 gtacgagctc gggcgtagag ttcttaatag tgtttacatc tacgttcgaa gcttcggctg
     4261 gtaattcgct tgcgtcaacc cgagctttca cagttggatc agttcgaaaa aagatatcgg
     4321 ctccggccaa ggttatgaaa atgagaagaa acaaaaatgt cccgaaaaag gtgacgttgt
     4381 tctccttgcc ttcatacttt agccccatga agtaaagtgc gaccattacc gccttcaccg
     4441 tggcaatcaa catggctagg agaatattat tcacaccccc gaagtcgata taagaagacc
     4501 cgaccgtaac tacggtgagg actaaaaggc caatccacac gctgtagtaa acctttaagg
     4561 gtgtcacatg gtgcgaatgc ttgtgattgt cgtgcgatga cattgtcgtc tctcctcaac
     4621 caattaaata aagcagcggg aatagataaa tccaaatcaa gtcgaccaag tgccaatata
     4681 ggcccgcgat ctccaccgga gtgtagtatg ctgagctaaa ctcacccttc atcgttctcc
     4741 gcaataccca gatgatcagg cccattccga tcaaaacgtg taaaccgtga agccccgtca
     4801 ttaagaagta tatcgaaaag aaaatcggcg ccttcggatg ggtgatgtcg gggttgttat
     4861 aaaacgcacc cggcagcaag cctatgtgga tcttgtggga atattcaaaa tatttaatga
     4921 ctaaaaacat acacgcgaaa aagagcgtgg ccatcatcag cttggccgat ttagcgcgct
     4981 catttcgctg agccattccg actgcaagcg ccattgtaag ggagctgcag atcaacacca
     5041 cggtattgac ggcacccatg attttattca aagcatggtg ggcttccatg aacatttcgg
     5101 ggtacatgcc acggtaaatg ccgtaagcga tgaacaatcc gccgaagagc ataatttcgg
     5161 tgactagaaa cacccacatg ccgaacttcg aggtttcgaa ttcgtggtcg gcactttcaa
     5221 aatgatgggc gtaatgatga gagcccgtag tagaggcgtc gtgactcatg gtttaaaccc
     5281 tttccgattt atactcgtaa ggccagttat caaccgttgg ttgaccgtga aaattatgcg
     5341 taatgggcgg cgatgcagcg tcggtccatt ccagagtttt ggctccccat ggattttggg
     5401 tggccttttg accttttttg gcagcccaaa agagatagcc gagagttaac atcaatccga
     5461 aaccgaccat caatgaaccg attgtcgaga cttgatgccc aagggtgtag accggatcat
     5521 aattgtagta ccggcgaggc atcccctttc caccaatata aaactgcgga agaaagagta
     5581 ggttaaagcc aaagaacacg agcgcccaag caaaacgccc aagtttctct ggatacatac
     5641 gtccgaaaaa cttcggccac caatagtgaa gccctccgaa aagcgccatc acggtaccgc
     5701 ccatcatgac gtagtgaaag tgcgcgacga taaagtaagt gtcgtgcaga tgcacatcga
     5761 tcgaaagcgc ggcgaggaaa attccggtca aaccaccgat agtgaacaag aacaagaagg
     5821 ccaaagcata cagcatgggg acgtctagtt tgatcgaacc tttatacata gtggaaagcc
     5881 agttgaagac cttaattgca gtcggaaccg caactaacac tgttaggaac gaaaaaataa
     5941 tgttcgaaaa atctgactga ccgctcacga acatatgatg gccccagact agaaaactga
     6001 tcgcggcgat agctaaacta gaataggcaa tcgcacggta tccgaagatg ggctttcgcg
     6061 aaaaaaccgg catgatttcc gaaattatgc ccatgcccgg cagaatcata atgtaaacgg
     6121 ccggatgaga ataaaaccaa aagaagtgtt ggaacaaaac tggatccccg cccagggcag
     6181 gatcaaaaat tccaaccttc aaaacgcgct cggcgactaa aagtagaaga gtaataccga
     6241 tcaccggagt ggccagcact tgaataatac ttgtagcgta agtagcccac acaaatagcg
     6301 gcattcggtg aaaagtcatt cctggcgccc gcaatttatg gatggtcgca atgaaattca
     6361 atccagttag aatggaggac atccccataa taaaggcgcc caaaaccgtg agaatcacac
     6421 cagtctgaga cttaatgctg tatggggtgt aaaaggtcca cccagtgtct acaccttgac
     6481 ccgcaagaat tgcgacaact gcgagagtcg ctccgaataa aaatacgtaa tagctaaaaa
     6541 ggttcaggcg cggaaaggct acgtctttcg caccaagcat taaaggcaaa agaaaattgc
     6601 ccagtgccga tggaatcgcg ggaataataa acatgaacac catgatcgcg ccatggagcg
     6661 tgaaaacctt gttgtaggta tcaggtccca tgatggtacg ggttggtccc cagtgctcta
     6721 accgaatgcc gatcgccaac aatcctgcga caccaaagaa aatcaaaact gtaacgagat
     6781 acatgatccc gatacgttta tggtcaatgg tagagagcca agaccagaca gttttaccat
     6841 catttaaata atctacatcg tgtccatgcg aggcgcttgc gtcgtgtgcc ataactcttg
     6901 actcctaatt tctgatcact tcaaactttt aatataggcg attagatcgt tgacctcttc
     6961 gtctttcaat aatcctgcaa acggaggcat tgaaggagta taccccttca cgatctgggc
     7021 attggggtcc atgagcgact tacgaatgta gttttcatct actgtcacca tggtcccatc
     7081 ggccatttct tctttcttcc cgaaaattcc tttgtagctc ggtcctacca tcctcgtgcc
     7141 gttgtcggag tggcaggcaa tacagccttt gctagctgcc aaggccttgc cgcgttcggc
     7201 cggagtctga cttggttggg aacttccgtt acccgttgcg gcagccaccg atgtcggagc
     7261 gcctaccgcg ccaattattt gatttagatt cacatgggcc ggcaactttc cagttcgggt
     7321 ccactcgtca aattcttcgg gttcgacgac gaccgctttc gcgagcatgt ccgagtgagc
     7381 agttccacag tactcggcgc aaaaaactat gtgtacacca ggttgagtcg ctatgaacga
     7441 aagtgtggtg aatcgtccgg gcactacgtc ttgtttaatg cgaaaattcg gaatgaaaaa
     7501 tgagtgcaac acatcttcag aagtcatgat taaccgaacc ggtttcccct tcggaagcac
     7561 cagttcattc aaggtttttc gaccattggt gtattcgata ttccaaagcc attgacgacc
     7621 tataatgttg atttcaactg ggtccttcat cggtgcgcgc atttcgcgat aaccctttag
     7681 tccccaagca aaaacaaaca gcatcagcac caaaggtacg accgtccaaa tcgtctcgag
     7741 cgtgtggctc ccaacaatat aggcggtctc acgcccggtt ttagaacggt gatacttcac
     7801 tgtgaaataa accatcacca ccgttacgag gatcaggaaa aacacgctta gatatacgag
     7861 aaagtcgtaa agtatgtcga ccgtcggtgc aattgtcgac gactgcggga gtccgaccgt
     7921 gctcgcatag gcactttgcc aaaacattat gagttcctcc tggttgtagg ccgagttgga
     7981 cggtgactac gttgaatcac ggtcccaaga attcctgcta agattaaaac tgtaagccca
     8041 ccgcccgcac gcataacatt gcgagcataa agcgaataag actttgcctc tggatcatag
     8101 tggtaacaaa acaaaatcat acgatcgaca aaggtgccga ttttgcgccc accggcttcg
     8161 agcagggcaa atcgcaagtc gcgagccgca aactgaatac cgtagagata gcgcgatact
     8221 ttcccttcgg gcgtcaacac tacgatcgct gcactgtggg cgtattgatc cgtggagtct
     8281 tcccaacggt atttaaaccc aacttgttca gcaagtttct tggcattggt gtctaccgca
     8341 tggggactgg ttcttggttg atcgttgacc cagaaatgcc acccagcagc tgcatcaggt
     8401 tttccaagac tttcaatgtg ggaattcttt ttggcagcag ctaaatccgc atcttctcgc
     8461 ggatcaatgc tcaccgttac aacatcgaac tcttgaccga tcttgtattg aagttcttta
     8521 aatccgtcgg tcacgccatt taaaaaataa ttacataaat tggggcatcc ataatacgcg
     8581 agcaagagca ttaccggctt cttgccgcta actaccgact tgagcggtac tacttcaccc
     8641 ttttcattct taaaggttaa attgaggtcg acctgagccc caaatttctc ctcgatgccg
     8701 attccctcaa gctctttagg taacttttcc gcagcatgcg ctcgcggtgt aataagggcg
     8761 tctgcgatca taaacaaaaa gagtgccaac aaatacagaa tagaaaaacc aaacgtgcgc
     8821 cacgctgtag ccttcaattg ctcggcactt accttagtga ttttccaagc caacttaagg
     8881 aaaattccac ccaataccgc tgcaccaaaa aaataaagcg ctccaacagc tccggttata
     8941 aatggcacta aagtgagcgg aacaagcgct aaggtgtaca gaaatatctg ccttctcgtt
     9001 tcttccactc cagcgaccac cggcaacatt ggaaccgata ctttagcgta ttcgtctttc
     9061 aagcagagcg cgagcgccca gaaatgagga ggggtccaca taaaaattac gagaaacatg
     9121 acaaacgcgt caaaactaat atttcctgtc gccgctgccc aaccaatcat cggcgcgctt
     9181 gcaccggccg caccgccaat gacaatgttg taaggtgtcg aacgtttaag ccacatagtg
     9241 taaaccacga cgtagtaaaa aatcgtagca acgcccaatg cggccgccaa aaatccgcca
     9301 aatgtataca aaagaaaagt cgctactgcc cccgaaacac atgcaaaaat caacgctgcg
     9361 ttcggagaaa ggctgccgtt cggtaaaggc ctcttggctt tggtgcgttc cataaccgaa
     9421 tcaatgtctc gatcgagaaa ctgattaaat gcattcgcgg atccacctgt aagagcaatt
     9481 cctaaacaga ccatccagag cgccaatggg tgcgataaga gagttccctg aacgatcatc
     9541 gcagttaaac cagtcaaggc gaacaatagt ataattcgag gttttgtaag ctgtacaaag
     9601 gcccctactt tgcggccaaa ggaaagctta cccttaacga gtgactcacc accgactgtc
     9661 tgctctaggc ccgttccggg caacgaatta gtttgttcga cggatctcat aaaacaacac
     9721 cacaaaactt cctattaaca atgctgcgac tgacaaatgc gccacactca ttaagagcgg
     9781 aagctgccaa atcacatttc caatgcctaa aaatatttga atcccaagca acacaagagc
     9841 gaaaacgctc cagccaacga ccgaaggcga tagttgtttt ttcttggccc aaaccgcaac
     9901 aaaaaaagtc accgccacaa gggtaaccac aatggcccct atgcgatgaa tcatttgaaa
     9961 gcgaatgatt ccttcaaacg gtggccacca aactcccaag cattttggaa agtcaggaca
    10021 agccaaacct gcataatttg aagccacccc gccacccatg agcgactgcc cataaatagc
    10081 aaaaagcgca attgcggcca aattagatag accagactct tcggcccgat tgcgaaagca
    10141 aaccgcaagc aatggttcag tacgtcctct gccactgagt tcggaaaatt taatcgaaat
    10201 caccactaac gcagcgaaaa atgcgagccc caccgcaaga tgcgatgata cccacccggg
    10261 atgcagcaag cccaacaccg tcatcccacc caggactact tgggcagcca acatgattag
    10321 ggcggctata caccatttgc caactagctg acgcagagag cgattggtaa gaaccaccac
    10381 cgaaagagtc aaaagcccaa tactaacgaa gcctgcgacc aagcggtgaa accactctag
    10441 gaacacttga taatccatcg gtggaaccac atgcccatgg caaagaggcc aatcaggaca
    10501 tgccaggccc gcatcttcat tccgcacaag agagcctaaa ctgatcagaa agaacgtcac
    10561 tccaaccaaa agttttgaca aaacttgaac aaaggaaagg gtccaaaagc gattgcctgg
    10621 agctaagtcc ttcgaagctt tgccggttcc aactccaaac cctggctgtg ttaattctac
    10681 tgacatcatt acctctaaat ctattgagcg tgacccagac actgcgaaag tcgcggatct
    10741 ttaaccggta ctgctggaac tcgttcaaga aaacgaataa ccgacaaccc aaaaacaccg
    10801 gcaaatgccg caaacgttcc aagttcgatc caaccaaaaa ctgggccgtc tttaaagaaa
    10861 acgggcatca cgagaaagta cacgtccatc cactgcgcga gcagcatgaa aacggccatt
    10921 ccaaaaagca ccttttcgtt gcgtttcacg ccacgcggaa gcaacgaaaa aaatggaatt
    10981 gcgaaatgca cgatgagaag agtccagaaa taaggccacc acgatccatt gaatcggcga
    11041 atgtaataaa ccgtttcttc aggaaggttg gcgtaccact gcaacattaa ctgagagaac
    11101 atgatgtaag cccaaaatac ggtaaaggcg aacatcattt tgccgatatc gtgaatatgg
    11161 ttttcgttaa ccatttggtc ggtgagtaca ccctgacgtc gaccataaat taccaagatt
    11221 gcgagcagac taaatccgct gaacactaat ccggcaaaac agtaaattcc gtagatcgta
    11281 ctaaaccaat gtggttcgag cgacattaac aaatcaaaac tcgcgaccga aaacgaaata
    11341 ccaaacaacg gcaaaaacac tgctgccgtt cgaacattgg ccaaagtgtg tttatgatca
    11401 ccatcgacat cctgcgcgag tgaatttttt cgcatcttga gccctaggcc aatctagatg
    11461 acaaaaaata aaatcatgcg cacgacgaaa aacttcatgt tcaagtacgg cgctttaaga
    11521 tgcagcaaat gatcgttggc gaccacttca gtgtgcgtcc actcgtatag gtgatgggtg
    11581 ccaaacatca acaccacgcc aagcactagc gctacgggca aataggacat aagtccttcg
    11641 gcaaatcggc gcaccgtcac gctccaatag gcattggtga tgtgttgcag agccacgaaa
    11701 aaagctccga acaagctcag ggccaaaaag tagaaatagt tcactaaaaa attgggccac
    11761 gctcttgccg gctgctttaa cagtgcggtg acagtcaaaa tcgaaccgat cactgccaac
    11821 acaagaagtg tgcttttggc ccctgccgaa aaccgcatcg accttactga acttggctga
    11881 aactctgtgt gtgtatgatg tgacatggaa tcaacgtcct cccgctgctt tcagatcttc
    11941 ggccgttgga ttgagcgacc tctgaagggt gcgaacataa tgaacgatcg cccaacggtc
    12001 ttccggcgca atctggctag cgtattttgg catcaaattt tgaccatctg aaatgacgtg
    12061 aaaaatctga ccatcgctcc aatccctaac tttttcacta tgaagcgtcg gtggccgggg
    12121 aaacttaggc acaatactac cgtcaccttc ccctttgcgt ccgtgacaga ccatgcagta
    12181 ggtctcaaac attaatttcc cacgagctaa aacaggtttg gtcgaacgta gcggatttcg
    12241 caaacccttg gccgcatcgg ggtcaccacg gtgggcgtac ggttggtatc cctgaggcac
    12301 agtgccctcg ggcggaacgc ccatcccatt gccgtcagaa tctcgttctt gtcctttaac
    12361 ggccggcgaa tccgccatat gaggcatgaa ttcaaatgcg ggctttttag ggtttttggt
    12421 acaccctgct gcgaaaactc caagagcggc caccaaaacg aggcgctcac accatgcgga
    12481 cttcattcgc acctgccttt ttaaaaagtt cggaaatttc agcctctcgg taaagaggat
    12541 cgtcggatga aataaacaaa gcgaagtggt catccgtcaa acggggatcg attatcttcg
    12601 cttttaaatt tggaagaccg caagtgatga tcatcacacc ggcagtcgca agcccagcga
    12661 gcagaatagt taattcgaac atgataggta caaacgccgg caatgaaatg aatggcttac
    12721 caccaacatt tagtggccag tcgaaagccg atgtccaaat ttggaaacca gtaccaatcg
    12781 ccaaaccagt gaggcctgca ataaaagtaa cccacggtaa aaacgatcgt ttcaaaccca
    12841 ttgcgtgttc aagtccatgc accggaaagg gagtaaatgc gtcgaagcgc ttgaacttgc
    12901 gatcgcgcac ttgctccgca gcgtgcaaca actggtccgg gtctttaaaa atacctacga
    12961 tgcctttcat ttgtgagcct cttcatgttt tgcgccgtga attacggact tcacttcagc
    13021 catcgcaatc attggcagaa ctcggcagaa cgccaggaac aacgtgaaga acaaaccgaa
    13081 gcttccaacc atgattccaa tttcaaccca cgtcggagta aacatactcc agcttgacgg
    13141 taaaaagtcg cgatgaagcg atgtaacgat aatcacaaat cgttcgaacc acattccgat
    13201 gttcacgaaa atagtaatga caaacatggc cttgaggtta cgacgaatgg atttagacca
    13261 aaacaactgc ggaataagca cgttgcatgt aaccattgtc cagtaggacc agccgtaggg
    13321 gccgaatgcc ctgttaatga acacaaaacg ctcgtattcg tttccgctgt accacgctac
    13381 aaaaaactct gtgccgtaag cgtagcccac catcaaaccc gtgagcatga tgactttgca
    13441 catgttttct aagtgaacat ggcgaatata agatttcagt tgtggcaaaa cctcacgggc
    13501 gatcaaaagc agcgtgataa ccatcgcgaa gcctgaaaaa atcgcgcccg ctacaaagta
    13561 aggaggaaag atggtggtgt gccaaccggg caaaattgaa actgcgaagt caaaagatac
    13621 gatactgtga accgaaagca ccaggggcgt cgaaaggccc gcaagtaaca tgtatgcggt
    13681 ttcgtaatga ttccacgcac gtgccgaccc ggtccaaccg agagacaata tcgtgtaaat
    13741 ggttttacga atcttgtttt tcgcacgatc acgcaccgac gcgatatctg ggattaatcc
    13801 aatgtaccaa aacaccaacg aaatcgtaaa ataggtcgaa actgcgaaaa cgtcccacag
    13861 caaggggctg cggaagttta cccaaagatc acgagagttc ggataaggaa acaaccaaaa
    13921 ggctgcaagc cattggcgac cggtgtgtag gagcgggaat atcgccgcgg tcataaccgc
    13981 aaagatcgtc attgcttcag cagaccgatt gatcgaagtt cgccattttt ggcggaacaa
    14041 aaaaagaatc gcagaaatta aagttccagc gtgaccaatg ccaatccaaa atacgaagtt
    14101 ggtaatatca aaggcccatc cgacggtgcg gtttaacccc cactgtccaa tacccgtcgc
    14161 tatcgtcagc cccatgcaaa agaggccgat gaacatgacg atattggcca ccataaaagc
    14221 tttgaaccac aacttcgtcg ggaacctctc cactggatcg caaacgtcgt tggtaacgtc
    14281 ggcgtagctt ttggaggggt caatcagcgg ctctttagcc aaggacgccg catttctaag
    14341 aactacttct gttgagctca tgcgtttttg ttcctgattt ttgtcaaata ggtcacactc
    14401 ggtttcacgt ttaattcttc aagcacgtga tagccgcgtg gatctgcgtt gattttagca
    14461 atacgcgaat ttttgtcgtt tacgtttcca aatacaattg cgtcggacgg acaactttgt
    14521 tgacaagcag tctgaacctg gccatccacc accgtggtcc ccaggtcctt cgctcgatct
    14581 ttgccgtcac gtatgcgctg catgcaaaaa gtacattttt ccataatacc gcgagtacgc
    14641 acggtgagat ctgggttgta ggccaaatgc aatggtttcg cgacttcggt gaacgtaaac
    14701 caattaaatc ggcgaacctt atatgggcag ttgtttgcgc aataacgagt tcctacgcag
    14761 cgattgtaga cctgctgatt aagtccttcg tcatcgtgca ttgttgcgag aaccgggcaa
    14821 acagtttcgc aaggtgcgtt ttcgcagtgc tggcaaagca ttggttggtg cacggtttcc
    14881 ggattatcgt cgctcccatt gtaataacga tcaatgcgta tccagtgcat ttcacgacca
    14941 gtttcgacga actttttgcc cacaactgga atattatttt ccgattggca cccaatcacg
    15001 caagcaccgc aaccggtgca ggaacttagg tcaattgcca tcccccaacg gtaacccgga
    15061 tacttgtgtt cctgccaaag ggtaaccaat gggtgaccat cgggatttcc agccttcggg
    15121 ttagatacgt attcaggata atccgcttca cgtacgatcg gacgcccctc aaggcgatga
    15181 tgcatctgcg tagctgcgag tttctcacgt ttgctggttt tttcgatagt caccggaatt
    15241 ccggacaaat caagagtttc ggcattggca gaggccaaaa tgtaagcgtt tacccccaca
    15301 ttctcagaaa ctcggcccga gccgattctg ccatagccca cagcgagcgc cactacttga
    15361 tcatgcacac ctggttgaac gtgggcgggc acatgcaatt cgccggaaga cgtctttaat
    15421 cgcagaacat caccttcgac gactcctagc tttttagcgg tagcaactga aagtgctgca
    15481 tagttatccc aggtaatttt cgaaatcggg tcgggcaatt cttgcaacca accgttgttg
    15541 gctgaacgtc catcgtacat tccaattttg ggatacagta cgagtgtcat ctcgcttgtg
    15601 acgccgccat tttttgcagt ccactcagcg acacttcgta aactcgacgt atcaaaactt
    15661 cgagcgcgag aggttccaag ttcgcgactg cctttggccg ccactccatc aaacactccc
    15721 tcacgcagca aactttccca aaaaagcgca aacggcgccg aaatgctaaa gttactgtat
    15781 attgtgtttt tccaattcga cttaacgaac tcgtgccaag taggcgaagt aacaagagca
    15841 cccgacattc ccagtttttt tgcccatgcc atgagtgtct cttcaaaaga ccgagtgttg
    15901 tacaagggcc taatggtcgg ttgctgaatt gaaaataaat ctctctgagc ttcagcgtcg
    15961 ccccaagctt caagcgcatg agaagcggcc gctacgtagt ctgacaatct ggccgtttca
    16021 tcaaacttgg tgcccacgta aacaacggtc cctaccttgg cgagagcctc ttcaaaacct
    16081 gcgtttttag gaagaccata agcggggttc acccccgaaa taaccagaac atctaccgcc
    16141 ccggctttca tgtcgcgaat aagtttggtg agagcttcga agttcgtacg tcgctcaaac
    16201 ggggtcgtag tgccatctac ggtgttacct tcgttttcga gcgctgaatt caatagcgcg
    16261 accactactt ctagagcatt tcctgccgct ccttttagcc catgccccgc tcctacaacg
    16321 attgatttac cgcgatcggc ccagagctcg ttggcgactt ttttaagtgc agatgcatcg
    16381 atccctaaat cttttgacac cgtggaa
//
LOCUS       DAOMXO010000002        14682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_2264_length_14682_cov_5.079579, whole genome shotgun sequence.
ACCESSION   DAOMXO010000002 DAOMXO010000000
VERSION     DAOMXO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 14682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14682
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2264_length_14682_cov_5.079579"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLH57_00085"
     CDS             <1..563
                     /locus_tag="PLH57_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK01034.1"
                     /translation="TMLNHASPVLVLVLSWWFLGERLSKKALFYVVLAFLGVLLVLKP
                     GFRINNLAGFLGVCSAFTAAIAYVAVKKATRKVSPEYIVFTFAVVGTLASAIPAYRSF
                     IMPTALQGVYLLGVGIFATLAQIAMTRGYSLLPASVAAPMLLITVVFSTLWGWLFWGE
                     LPDALSALGAVCVAVGLTLAYRHRTR"
     gene            579..3365
                     /gene="ileS"
                     /locus_tag="PLH57_00090"
     CDS             579..3365
                     /gene="ileS"
                     /locus_tag="PLH57_00090"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRK01035.1"
                     /translation="MDYKSTLNLPQTEFPMKASLPQREPERVAHWESQATYKKMIAHT
                     RSKPLFTLHDGPPYANGNIHLGHTLNKVLKDIVIKYQNMAGKHAEFIPGWDCHGLPIE
                     LGVEKQLRDKKIDKTTLSRVKIRDLCKEYALTYVEKQKEQFKRLGILSDWDAPYLTLA
                     DDYVANIVRELGRISNAGNLYKGNKPVYWCASCATALAEAEIEYHDHTSPSVYVKFFL
                     QATSIEKCSAIAKLVNGKPVAAVIWTTTPWTLPANLGISAHPDFEYALIDVGNEVWII
                     AEGLREKFLTEIERTEPAKVLGKINGRQLENLKADHPFMKRSSLLMVGEHVTLEAGTG
                     LVHTAPGHGVDDYKIGLRYGLEVLAPVDAKGAYTAEVPDYQGKKVWESNPLIIDRLNS
                     SGHLIHQKKIKHSYPHCWRCSNPVLFRATPQWFIGMERTKLRERAVQEIHKTEWIPAW
                     GINRILGMVEGRPDWCVSRQRVWGVPITVFYCESCEDSVHSKESFDFVADVIDKNGPN
                     AWYEWPVEKLLKPGTRCQCGNSDAAKFRKETDILDVWFDSGVSHAAVLAAPKNKSKVE
                     WPADLYLEGSDQHRGWFQTSLLTAVEARGAAPFKRVLTHGFVNDREGKKMSKSKGNVV
                     DPLDFVSKNGAEILRLWVVTEDYRNDINFSSETIDRISESYRKIRNTFRYILGNINDF
                     ESSMLPKSESDYCDLDQWALARTEKFLARVKAAYDSYEFHQAYHALVNFCATDLSALY
                     FDILKDRLYTSAKNSKERRSSQSALYRIGLALASSLAPILSFTSEEVWGYLKQTGSVF
                     ESDFPVWSASSERENIAMRVEKALELREPINKKLESLRAAKEIGHSLEASVEVKAPDT
                     VVAQVEQVREDLARMFIVSKIILKPGNSEGVEVLAKKADGEKCARCWTYSQHVGTDSN
                     HPQLCDRCTTAVKA"
     gene            3362..3871
                     /gene="lspA"
                     /locus_tag="PLH57_00095"
     CDS             3362..3871
                     /gene="lspA"
                     /locus_tag="PLH57_00095"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRK01036.1"
                     /translation="MKLKYLILLAVSGTIISIDQLTKQIVVQRFLLGESVEVISGFFN
                     LTYVHNPGAAFGFLAQAHSGFRVPFFIVVPMIALAIIGFIFRKVDDRDLKLSGALSLV
                     IGGAVGNLIDRVAYNYVIDFLDFHWNYAIHFPAFNVADIAITVGVAILIIDIFQKESA
                     QAKNVSNAA"
     gene            3852..4634
                     /gene="lgt"
                     /locus_tag="PLH57_00100"
     CDS             3852..4634
                     /gene="lgt"
                     /locus_tag="PLH57_00100"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRK01037.1"
                     /translation="MYPTLLEVAGFPIHTYGVLIAIGFVLCVFVAKREAQRTGLQAEK
                     IVDAGFWSLFVGMIGARLLYVITRWDYFRLVPLESFYVWEGGLVFYGGPLFVIPFFFW
                     YARRHQIPIFKLMDIGAISLPLAHAFGRLGCLSTGCCYGRPTNSTWGIKLHSELVEPY
                     LRGVNLHPTQLYESAALFCLTAILFVQHRKKSFDGQTASLYLIIYPVIRFIVETFRGD
                     TVRGFVFGGALSTSQFISIWILFSGILSYVYFRSRSLKKAVA"
     gene            4634..5479
                     /locus_tag="PLH57_00105"
     CDS             4634..5479
                     /locus_tag="PLH57_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRK01038.1"
                     /translation="MRWVLVACLLFYSTVSSASENFSRIVVLMPHLAESLFNFGILEE
                     IVGVPEFTTVPESQKKRIKFIGPYHRISIESVLALRPTIVLASKDGNLLSNVDELTAL
                     GINVKTFDPRTLGGVVATLVSLREIADQVRPKLKLQNERKLLDFKKQLTKLRGSLPNA
                     RVFVQIGFDPLVTVSDRSFLGEFIHHLGLENIYAKELAAYPRPAIEDVVSRRPDIILI
                     VNLTNSAQVGEKNRAVWAKFRSLPGVSNDRVIMLAGDRIVKPGFEMLQGAGDLVTQLK
                     RNNGK"
     gene            5469..6398
                     /locus_tag="PLH57_00110"
     CDS             5469..6398
                     /locus_tag="PLH57_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRK01039.1"
                     /translation="MANSAKIIGVLLLALLAFVVAVLVGPAEMTSEIFWQLRLPRAIG
                     VASVGAGLAVAGVLVQSSFGNPLADPFTVGIASAAALGAVVGAVVFRGSFLGFGPGAM
                     AFVFALGFSFVMTAWMRRGIRSAADILLVGVVCGFFFTACATLILSLSEPHALAHSLS
                     WLMGSATYIELPWALGVAAITTVVTLVAWLQWRAMDLVTVNELTARSMGVDVKKLRLL
                     LYVCVAVIVAISVGVAGVIGFVGLVVPHALRRLGVRSTVTLIPAAFFSGAALVLSADA
                     LARTIIAPTEVPVGVILAIIGAPVFLWLARRSL"
     gene            6400..7191
                     /locus_tag="PLH57_00115"
     CDS             6400..7191
                     /locus_tag="PLH57_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK01040.1"
                     /translation="MSIDFSNFSFTYAGGSKAAIQDICHEFESGSYWCVAGANGSGKS
                     TLLKSVCGILDGTVGKGTVKIEGREIRSIDRVNLARTVAYVPGGAGIQFPITARELIA
                     QGRFPYKQRLSIGLSEEDETKIQSIVALLGLEKNQNRLVSQLSWGESALVMLGRALAQ
                     ETKWLVLDEAVSSLDLGVQRRVFSVLEKMHRDRKVGILFVGHDLNLAAEFIPKLLWLH
                     EGKIIKSGPFNEVLSDDLLSYVYGKDAGLSVGQNPKNGKPKIFFS"
     gene            complement(7210..7956)
                     /locus_tag="PLH57_00120"
     CDS             complement(7210..7956)
                     /locus_tag="PLH57_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01041.1"
                     /translation="MLNDTVTLNPSMLAFQQVASLSGTFNWINSPTGAGTPDNTNQTF
                     NVSVIDGKNQYASAGLSFTRRHDADFVHVAVAKRVLPFMGIGLGGKRYSTRDNRLAAT
                     GRAISGVDGGASVSFAIPETVIATPIQIGLVADNLIHRASDEVLTGPRTYGGGVKVNV
                     SKILLLYGDLVEKFWHDRGAYPVYSGGAEIAMGGSFYARGGLFGYREKGWSAGGGWVG
                     PKLGLSYGYQSKREDAVRSFDHAVTMDIYM"
     gene            8131..8502
                     /gene="dksA"
                     /locus_tag="PLH57_00125"
     CDS             8131..8502
                     /gene="dksA"
                     /locus_tag="PLH57_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HRK01042.1"
                     /translation="MTKAKLEYFRKILTKWLEELRKDMQQTVSQISHDDEVYADWTDV
                     ASVETDKTMQLKIRDRERVLMSKIEEALRRIDDGTYGECERCDEPISEPRLKARPVTT
                     LCIDCKSEIEGTEHRYRASYS"
     gene            8534..9406
                     /gene="galU"
                     /locus_tag="PLH57_00130"
     CDS             8534..9406
                     /gene="galU"
                     /locus_tag="PLH57_00130"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRK01043.1"
                     /translation="MRVRKAVIPVAGLGTRFLPATKAIPKEMIPIIDKPMIQYIVEEC
                     VGAGIEEIIFVTARGKDAILDHFDISFELETTLRAKGKADLLKICTEVSQMVTVTAVR
                     QKEALGLGHAVLTAKSAVGNEPFAVLLGDDLVVSKVPCIRQLISVFEKREQPVIGVMQ
                     VPREETGKYGIIGGKEIAGSGGKLIQVKTMVEKPKPASAPSNLACPGRYVLTPKIFEL
                     LEKTKPGAGGEIQLTDALAKLPDNGGLLACIFDGRRYDTGDKLGYIEATIAFALQRPE
                     FKKSVRELLTRYSQ"
     gene            9418..10302
                     /locus_tag="PLH57_00135"
     CDS             9418..10302
                     /locus_tag="PLH57_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01044.1"
                     /translation="MKQDFRRRSLKKLWWLPILATANFVGSSAQAYVDPSFHIIRQVA
                     RKHSAIDELRTRSRLTFYNRSGEVVRTFTEIVGFDNLSKVGGRIGDAEGVEVSFKSRT
                     HLATDKTRPLLYDLLYVRDSEILFERLQSLGLPLKSETDLYSEKEGILPYKPETFVNL
                     KQFEGQALLVIGEFSDRTPQLWVTKPERQSTSSAAVEGYQVARAVFFTSFDGPSGDTL
                     EFRFRNRKEYRGVESKTFFYPTRIEIIKDGYLWSTIDILELRSGAGASNLDTAHVKAA
                     DSNADSRELLEPYLKWIR"
     gene            10290..12284
                     /gene="priA"
                     /locus_tag="PLH57_00140"
     CDS             10290..12284
                     /gene="priA"
                     /locus_tag="PLH57_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRK01045.1"
                     /translation="MDSIKYAEVVVPKPLPGPLTYRIPVSLAGTIQPGSYVKVPFRSS
                     LADGFVVAVSSGTHCDEKIKIKDISEQPFFDLLFSDEDQKFFKWVSDYYQLPIGEVFH
                     SAFPKSIFKIPKKEKKTPEVSSSASGAPYNSRSAIEPTTEQINAINEIVSAVSSAEFR
                     SFLLFGVTGSGKTEVYLASAARALEIGKSALILVPEIALTPQLTSRFESRFGAQVAVL
                     HSGLTEKERREQWWSIRRGEKKVVVGARSALFAPLNDLGLIVVDEEHEPSFKQEDRLR
                     YNARDLALVRGKIASAAVVLGSATPSLETFFAAQNGRHRLLELKIRPNQRPMPSIEII
                     DMKGIDGHFSDPMRNGIEQALKNGNQCMVFVNRKGFAHFLLCTECGEVPKCLNCSVSL
                     TYYRASSTLRCHYCDFQIPSHSACKSCNSISLKQVGFGTEAIENELKVIFPAASIRRL
                     DAETVDTKKKLDEVLNDFRSGQIDILVGTQMLAKGHDFPNVTFVGIVLADTALHLPDF
                     RASERTFQLVTQVAGRCGRSDKPGTVVLQTFVPDHYTIINAAKQSYREFYETELAIRR
                     DFSYPPFSKMAQIEFRHLKENVARDQAFMAEALLRNAGADSMGVECLGPAAASIARIA
                     NQYRWQLLLKAAKSSALNGAIKTLRKAGVRFIDVDPVSTL"
     gene            12292..13131
                     /locus_tag="PLH57_00145"
     CDS             12292..13131
                     /locus_tag="PLH57_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01046.1"
                     /translation="MGKIEFSSERGKCPACRTENVILKEVSGIHVCEKCASKRFSTSV
                     RKRIEEAYNRQMDAKRREAWMQRMVVAKRGVKYYEEGKLLESLKCFRDYITILESRYR
                     VPPGGLHPSLFDVKKDAGELLLIAGVYWDMVKAYDHIKGHTGEMRGALNKFLEFSIDR
                     PHVILASETIRKYVASGSAVHVNDFKRAHEQLRSNLSKCFIATAVYGPKSDEVAALRA
                     FRDHFLNKNLIGRSVVGFYYAISPPLARLAVRQPRIADAIRPFLNLIVRSTKRASRLS
                     TDR"
     gene            13283..>14682
                     /locus_tag="PLH57_00150"
     CDS             13283..>14682
                     /locus_tag="PLH57_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01047.1"
                     /translation="MKTEKNLKDQKSQLDQLITILMSCFAELGVRPNSLFIEDTAVMI
                     YDAMSTRTRLYHRLEHCLSTPPNVDALGILAYLFHDVIYWQVDRARSSKLRQGIAPLE
                     LDDHGTVTLTGECGELGTAKISERNTLARLVFGFNEPVELDFSHGMNEFLSACVAENT
                     FKGLLDTWQIVQILCCIEATIPFRPALDGKTAIEKLAARLKKINIGYAFGKTEEEQKQ
                     AIRRAIDVANHDVGNFAYATTSTFLKNTWDLTWENNPALQRDTYSIKQYRSALQANIA
                     FYRSLQPRYVFSCLDGYPTRGHFEDIVGRTKKNITTGLQYIQVKLVACAIIESVADLT
                     GGDAPLMLFMGNHSQQGLIPKWADLLENIDPTIIKDELRKDVFKLLFEGRPEMKEQFD
                     VARSPISAHLYASLDAANFQKAAALADEFFVQRMSAKEFLQANPINLVYGPVLEKFKE
                     IVSSRKEALSALEKELR"
BASE COUNT         3763 a         3291 c         3684 g         3944 t
ORIGIN      
        1 gtactatgct caaccatgcc tccccggttt tggttttagt gttgtcctgg tggttcttgg
       61 gcgaaagact ttcaaaaaaa gcgctgtttt atgtggtgtt ggctttttta ggcgtgctcc
      121 tggtcctaaa accggggttt cgcataaata acttggcggg attcctgggg gtctgtagtg
      181 cctttacggc agcaatagcc tacgttgccg tgaaaaaggc cactcgaaaa gttagccccg
      241 agtacatagt gttcaccttc gcggtcgtgg gtacattggc cagtgcgata cccgcgtata
      301 ggtcctttat tatgcccacc gcgctccagg gggtgtacct gttgggagta gggatttttg
      361 caacgcttgc tcaaattgcg atgacacggg ggtattcgct actcccggca tcggtggcag
      421 caccgatgct gctcattact gtggtatttt caacactttg gggatggtta ttttggggcg
      481 aactgcccga cgctctaagt gctctgggag cggtctgtgt ggcggtgggc ctcacgcttg
      541 cctatcgtca tcgcacacgc tagaactgga aattctctat ggattacaaa tcaacgctca
      601 atttaccaca aaccgaattt cccatgaagg ctagcctccc gcagcgcgag cccgaacgcg
      661 tggcgcactg ggaaagccaa gcgacctata aaaaaatgat cgcccacacg cggtcaaagc
      721 cccttttcac gcttcacgat ggcccaccgt acgccaatgg caacattcac ttggggcata
      781 cgctcaataa agttttaaaa gacatcgtca tcaaatacca aaacatggcg ggcaaacacg
      841 cagagtttat tccggggtgg gactgccacg ggttgccgat cgaattaggg gtggaaaaac
      901 agcttcgcga taagaagata gataagacta ctctctcacg cgtcaagatt cgcgatctct
      961 gcaaagagta cgcactcacc tacgttgaga aacaaaaaga acagttcaag cgacttggaa
     1021 tattgtccga ctgggacgcg ccatacctta cactggccga cgactacgtg gccaacattg
     1081 tgcgggaact tgggcgcata tcaaacgccg gcaacttgta taaaggaaac aaaccggtgt
     1141 attggtgcgc ctcttgcgcc accgcactgg ccgaagctga aatcgaatat cacgatcata
     1201 cgagcccgtc tgtctacgtg aagttttttc tacaggccac ttctatcgaa aagtgttcgg
     1261 ccattgcgaa gctagtcaat ggcaagccgg tagccgcagt tatttggacc acgacgcctt
     1321 ggacacttcc ggcaaatcta ggtatttcag cgcacccaga tttcgaatac gcattaatag
     1381 acgtaggaaa tgaagtctgg attatcgccg agggattgcg ggaaaagttt ttaactgaaa
     1441 tcgaaagaac cgaacctgca aaagttctcg gaaaaattaa tggtcgccaa ttagaaaact
     1501 tgaaagccga tcatcccttt atgaaacgta gttcgctttt gatggtaggc gaacacgtta
     1561 cgcttgaagc cggtactggt ctcgttcaca ccgcgccggg acatggtgtt gacgattaca
     1621 agattggttt gcgttatgga ctagaagtct tggctcccgt cgatgcaaag ggggcctaca
     1681 ctgcggaagt gccagactat caaggaaaga aagtttggga gtcaaaccca ctcatcattg
     1741 atcggctgaa ttcgagtggt catctcattc atcaaaaaaa aataaaacac tcttatccgc
     1801 attgctggcg ctgtagcaat ccggtgttat ttcgagcgac cccgcagtgg ttcattggaa
     1861 tggagcgcac gaaacttcgt gagcgggcgg tgcaagaaat tcacaagaca gagtggattc
     1921 cagcgtgggg cataaataga attttgggaa tggtcgaagg gcgcccagac tggtgtgtgt
     1981 ctcgacaacg cgtttggggc gtgccgatca ccgtatttta ctgtgaaagc tgcgaagatt
     2041 ccgtacacag taaagagagc tttgattttg ttgccgacgt cattgataaa aatggtccta
     2101 acgcctggta tgaatggcca gtagagaagc tcttaaaacc aggaactcgc tgccagtgtg
     2161 gcaactccga cgccgccaag tttcggaaag aaaccgacat tctcgatgtt tggttcgact
     2221 cgggtgtaag tcatgccgcc gtactcgcag ctccaaaaaa taaatcgaag gtagaatggc
     2281 ccgccgatct ataccttgaa ggcagtgacc aacatcgcgg ttggtttcaa acctcgctcc
     2341 ttacggcggt cgaagcacga ggcgcggccc catttaagcg cgtattaact catggattcg
     2401 taaacgatcg tgaaggaaag aaaatgtcga aatccaaggg caatgtagtt gacccgctgg
     2461 attttgtgtc aaaaaacgga gccgaaattt tgcggctttg ggtcgtgact gaagactatc
     2521 gcaatgacat taatttttcg tcggagacaa tcgatcgcat ttcggaatcg taccgaaaaa
     2581 ttagaaacac ctttcggtat attttaggaa acatcaatga tttcgaatcg agtatgcttc
     2641 cgaagtctga gtcggattat tgtgatctcg atcagtgggc gcttgcacgc accgaaaaat
     2701 ttttggcgcg cgtaaaggcc gcgtatgatt catacgaatt tcaccaggcc taccatgcac
     2761 tggtaaattt ttgcgcaacg gatctgagcg cgctgtattt tgatattttg aaagatcgcc
     2821 tgtacacgtc tgcaaaaaat tcgaaagaac gccgttcgtc tcaatcggcg ctttatcgta
     2881 tcgggttggc tctggcttcg agtttggcgc caattttgtc gtttacctct gaagaagtgt
     2941 gggggtatct caagcagacg gggtcggtat tcgagtctga ttttccagtg tggtctgcta
     3001 gttcggagcg cgaaaacata gccatgcgtg tcgaaaaggc cctcgagctt cgtgagccca
     3061 ttaataaaaa gttagagagt ttaagagccg ccaaagaaat tgggcactct cttgaggcct
     3121 ctgtcgaagt gaaggcgccg gatactgtag ttgcccaagt tgaacaagtg cgcgaggacc
     3181 ttgctcgaat gtttattgtg tcaaaaatta ttttaaaacc tggaaactcc gagggagtgg
     3241 aggttttagc gaaaaaggct gacggcgaaa agtgcgcacg atgttggacg tattcccagc
     3301 acgttggcac ggactcgaat cacccacaac tttgtgatcg atgtacaacg gcggtgaagg
     3361 catgaaactc aaatatctca ttctactcgc ggtgagtgga accattattt caattgatca
     3421 attgaccaag cagattgttg ttcagcggtt tttattaggc gaaagcgtcg aagtgatttc
     3481 gggatttttt aatttgacct atgtacacaa cccgggtgcg gcattcggat tcctcgctca
     3541 ggcgcactct ggttttagag ttccgttttt tatcgttgtt ccgatgattg ctctagcgat
     3601 tatcggattt attttcagaa aagtggacga tcgagatctt aagttgtctg gcgcactttc
     3661 gttggtaatt ggcggcgcgg tcggaaattt aatagatcga gtcgcctaca actatgtaat
     3721 cgatttttta gattttcatt ggaattatgc gattcatttt ccggctttca acgttgcaga
     3781 tattgccata acggttggag tggccatctt gattatagat attttccaaa aggaaagcgc
     3841 gcaggcaaaa aatgtatcca acgctgcttg aggtcgcagg ctttccaatt cacacctacg
     3901 gcgtattaat cgcaattgga ttcgtattgt gtgtttttgt cgcaaagcgc gaggcacagc
     3961 gaacaggtct tcaagctgaa aaaatcgtag acgcgggttt ctggtcgctt tttgttggca
     4021 tgattggggc gcgattactc tatgtgatta cgcgctggga ttattttcgt cttgtgcctt
     4081 tggagtcatt ttacgtctgg gagggtggac ttgttttcta cggcggtcca ctttttgtta
     4141 ttccattttt cttttggtac gctcgtcgcc accaaattcc gattttcaaa ctcatggaca
     4201 taggggctat ttctttgccc ctcgcccacg cttttgggcg gctcggttgt ttgtccactg
     4261 ggtgttgtta tggacgtccc actaatagca cctggggaat taagctgcat tcagaacttg
     4321 tagagcctta tcttagaggg gtcaatttgc atcccaccca actctacgaa tctgctgcgt
     4381 tgttttgttt gaccgcgatt ctgttcgtgc agcatcgtaa aaaatctttt gacggccaaa
     4441 cagcctctct ttacctaatt atttatccgg tgattcgatt tattgtagaa acctttcgcg
     4501 gagacacggt gcggggtttt gtttttggcg gtgcactatc aacgtcgcag tttatttcga
     4561 tttggattct gttcagcgga atattgtcgt acgtttattt tcggtcacga tctctaaaaa
     4621 aagcggtcgc gtgatgcgtt gggttttagt cgcgtgcttg ctgttttact ccacggtctc
     4681 ctctgcttca gaaaattttt cgcgtatcgt ggtgctcatg ccacatttag cggaatctct
     4741 ttttaacttc ggaatactgg aagaaatcgt tggggttccc gagttcacca cggtgccgga
     4801 atctcagaaa aaacgaatca aatttattgg accctatcat cgaatttcca ttgaaagcgt
     4861 attggcgctc aggccaacca ttgtgttggc aagcaaagac ggaaatttat taagcaacgt
     4921 cgacgaatta acagccctag gaattaatgt taaaacattt gacccccgaa cccttggagg
     4981 ggtggtcgct acattagtat ctttgcgcga aatcgccgat caagtgcggc ccaaactaaa
     5041 gcttcaaaat gaacgaaaat tattagattt taagaagcaa ttaactaaac tgcgcggctc
     5101 attacctaac gcccgagttt ttgttcaaat tggatttgat ccgttggtga cggtgtccga
     5161 tcggagtttt ctcggagagt tcattcatca tttaggactt gaaaatattt atgcaaagga
     5221 actcgctgca tatccgcggc ccgcaattga agacgttgtg tctcgccggc cggatattat
     5281 tttgatcgtc aatctaacga attcagctca agtcggtgaa aaaaatcgcg ccgtatgggc
     5341 gaagtttaga tctcttccag gggtaagcaa cgatcgagta ataatgttag ctggtgatcg
     5401 cattgtaaaa cccgggttcg aaatgcttca gggcgcaggg gatctggtta cacagttaaa
     5461 gaggaataat ggcaaatagc gccaaaatta ttggggtcct gcttttggcg ttgctcgcct
     5521 ttgtcgtcgc agtcttggtc gggccagccg aaatgacgtc tgaaatattt tggcaattgc
     5581 ggttgccgcg tgcgatcggc gtggccagcg ttggcgcggg actcgcggta gcgggcgtgc
     5641 tcgttcagtc gagtttcggg aaccctttgg cagacccgtt tactgtcggt atcgcctccg
     5701 ccgccgctct tggagcagta gtaggcgcgg tggtttttcg cggaagtttt ctgggttttg
     5761 gacccggagc aatggcattt gtatttgcct tagggttttc cttcgttatg acggcatgga
     5821 tgcggcgtgg tattcgtagc gccgccgaca tattattagt gggagtcgtg tgtggatttt
     5881 tctttaccgc atgtgccacc ttaattctct cgctctcgga acctcatgct ttggctcatt
     5941 ctcttagttg gcttatgggt tcggccactt acatagaatt accgtgggca cttggtgttg
     6001 ctgcaataac gaccgtcgtc actttagtgg cgtggcttca gtggcgcgcg atggatttag
     6061 taacagtcaa cgaactcacg gctcgcagca tgggggtaga tgtaaaaaaa ttgcgactcc
     6121 tactgtatgt ttgtgtggct gtgatcgtcg ctattagtgt gggagtcgct ggcgtgatcg
     6181 gtttcgtagg tcttgtcgtg cctcatgctc ttaggcgcct tggtgtgcga agtactgtaa
     6241 ccttaattcc agccgctttt ttttcgggcg ccgcgttagt cttaagcgca gacgctttgg
     6301 ctcggacgat tatagcgccg acagaagtgc cggtcggggt aattttggct attattggtg
     6361 ctccagtgtt tttatggttg gcgcggaggt cgttgtaaaa tgagcatcga cttttctaat
     6421 ttcagtttta cttacgccgg cggttccaag gcggccatac aagatatttg tcatgaattt
     6481 gagtcgggtt cttattggtg tgtcgccgga gctaacgggt ccggtaagtc gactctcctt
     6541 aaatcagtct gcggaatttt agacggtacg gttggtaagg gtacagtcaa aatcgaaggg
     6601 cgagaaattc gctcgatcga tcgagttaat ttggcgagaa cagtcgctta cgttccgggt
     6661 ggagcgggaa tacaatttcc tattactgcg cgcgaactta ttgcgcaggg acgctttccc
     6721 tataagcagc ggctatctat tggtttgagt gaagaagacg aaacaaaaat tcagtctatt
     6781 gtggcgttgt tgggtttaga aaaaaatcag aatcgcttgg tgtcacagct atcttgggga
     6841 gagtctgccc tggtgatgtt gggccgtgca ttggctcagg aaactaaatg gctcgttctc
     6901 gacgaagcag tttctagttt agacctcgga gtgcaacgaa gagttttttc agtactcgaa
     6961 aaaatgcatc gcgatcgaaa agttggaata ttatttgtag gccacgacct taatcttgct
     7021 gcggaattta ttccaaaact gctttggctt catgagggaa aaataatcaa aagcggacct
     7081 tttaacgaag ttttgagcga cgatttactc agttacgttt atggcaaaga tgcgggcctt
     7141 agcgtgggcc agaacccgaa aaacggtaag ccaaaaattt tcttttcgta aactcacgtc
     7201 ggggacgatt tacatgtaaa tgtccatcgt aacagcgtgg tcaaaggaac gaacagcgtc
     7261 ttcacgcttc gattgataac cgtaactcag tccgagtttt ggaccaaccc agccaccgcc
     7321 agcgctccaa cccttttccc gatatccgaa aaggccgccg cgggcataaa aacttccgcc
     7381 catcgcgatt tccgcgccgc cactgtagac cgggtaagcg ccccgatcat gccaaaattt
     7441 ttcaaccaaa tcgccgtaga gtaacaaaat cttacttacg ttgaccttta ctccaccacc
     7501 ataggttctg gggccagtaa gcacttcatc ggaagcgcgg tgaattaagt tatcggctac
     7561 caaacctatt tgaattggtg ttgcgattac ggtttccgga atggcgaatg aaaccgaagc
     7621 gccaccgtcg acgccgctaa tggcacgtcc cgtcgcggcc agtcgattgt cgcgagttga
     7681 atagcgcttg ccgcccaatc caatacccat gaacgggagc actcgcttag ccacagccac
     7741 gtgtacaaag tccgcgtcat gacgtcgggt aaaagaaaga ccggcgctcg cgtattggtt
     7801 ctttccatcg ataaccgaca cattaaatgt ttgatttgta ttgtcaggtg ttccggctcc
     7861 cgttggggaa ttgatccagt taaatgtgcc agaaagtgag gccacttgtt gaaacgcgag
     7921 catgcttggg ttcaacgtca ccgtatcatt caacattgca ccgcctctgc cgctctgcga
     7981 aaggctcaac gttcttgacg actgaaaatc cagcgccagt gtacttaaag gagttgtcgc
     8041 taatactaga cttattaact ttagaatgcg gggcattgaa ttttgcatgg ctttacttcc
     8101 ttatttccct agcatatagg agtccaactt atgacgaaag caaaacttga atattttcgt
     8161 aagattttga ccaagtggct cgaagagctc cggaaagata tgcagcaaac cgttagtcag
     8221 atctctcatg acgacgaagt ctatgccgat tggacagacg tagcaagtgt cgaaaccgac
     8281 aagaccatgc agcttaaaat tcgggatcgc gaacgcgttt taatgtcgaa aatcgaagaa
     8341 gcccttcgtc gaattgacga tgggacctac ggtgaatgcg agcgttgtga tgaacccatt
     8401 tccgagccgc gcttgaaagc gagaccggtt acgactcttt gcattgactg caaatcagaa
     8461 atcgaaggta ctgagcaccg ctatcgtgct tcgtatagct aaactgattt aatagcccta
     8521 tactctaaac atcatgcgtg tacgtaaagc ggttattccc gttgctgggt tagggaccag
     8581 gtttctgcct gccacgaaag ccattcccaa agaaatgatc ccgatcatcg acaaaccgat
     8641 gatccaatac attgttgaag agtgcgtcgg cgccggcatt gaagagatca tttttgttac
     8701 tgctcgtggt aaagatgcaa ttctagatca tttcgatatt agctttgagc tcgaaaccac
     8761 attacgggcc aagggtaagg ccgatctttt aaagatatgc actgaagtgt cgcagatggt
     8821 tacagtaaca gcggtgcgtc aaaaagaggc gctcgggctt gggcatgcag ttcttacggc
     8881 caagagcgcc gtaggtaacg aaccgtttgc tgtattactt ggcgacgatt tggtcgtctc
     8941 caaggtgcct tgtattcgcc agctcatcag cgtgttcgaa aaacgagagc agcccgtaat
     9001 cggagtaatg caggttccgc gcgaagaaac tgggaagtac ggtattattg gcggcaaaga
     9061 aattgcgggt tcgggcggga agctcattca ggttaagacc atggttgaaa agccgaaacc
     9121 tgcgagtgcg ccttcgaacc tcgcgtgtcc cgggcgttat gtactcacgc cgaaaatttt
     9181 tgaattactc gaaaaaacta agcccggcgc aggtggagaa attcaactga ctgacgcact
     9241 cgcgaaattg cccgataacg ggggcttatt agcctgtatt tttgacggac gtcgctatga
     9301 cacgggcgat aaattgggtt acattgaggc gacgatcgcg tttgcactgc agcgcccgga
     9361 attcaaaaag tcagtacgtg agcttttgac tcgatattct caataggaca ataaaagatg
     9421 aaacaggatt ttcgtcgacg atcactcaaa aaactttggt ggttacccat tttggctacg
     9481 gcgaatttcg ttgggagttc ggcacaggcc tatgtggacc ctagttttca cattattcgc
     9541 caggttgccc gtaaacactc agctattgat gagctgcgca cgcgcagtcg tctgacgttt
     9601 tacaacaggt ccggcgaagt ggttcgcacc tttacggaaa tcgtgggttt tgacaattta
     9661 tctaaggtcg gcggtcgcat cggagacgct gagggggttg aagtctcatt caaatctcgc
     9721 acccatctgg caaccgataa aactcgcccc ctgctttacg atcttcttta cgtgcgcgat
     9781 tcagaaatat tgtttgagcg gttgcagtcc ctgggtcttc cgttaaagtc cgaaaccgat
     9841 ctctattcgg aaaaagaagg gatactgccg tacaaacccg agacatttgt aaatcttaag
     9901 cagttcgaag gacaagccct gttagtgatt ggtgaatttt cagaccgtac cccacagctt
     9961 tgggttacca aaccggaacg tcaaagtact tcgagtgccg cagtcgaagg ctatcaagtg
    10021 gcacgtgccg tattttttac gtctttcgac ggtcccagtg gcgatacatt ggaatttcgt
    10081 tttcgaaacc gaaaagaata tcgtggggta gaatctaaaa cgttttttta ccccacccgg
    10141 attgaaatta taaaagacgg ttatctctgg tcgacgatcg acattcttga gttgcgatcg
    10201 ggagctggag cttcgaacct cgacaccgcc catgtgaaag cggctgattc aaacgccgac
    10261 tcgagagaat tgttagagcc gtacttaaaa tggattcgat aaaatatgct gaagttgtgg
    10321 tgccgaagcc tttgccgggg cccctaacct atcgcatacc cgtcagccta gctggaacca
    10381 ttcagcccgg ctcttatgtg aaagtcccct ttcggtcgag tctggccgac ggttttgtgg
    10441 ttgcagtttc gagcggcact cactgcgacg aaaaaattaa aattaaagac atttcggaac
    10501 aaccattttt tgatttgttg tttagcgacg aggatcaaaa attttttaaa tgggtttcag
    10561 attactatca gttgcctatt ggcgaggtat ttcacagcgc gtttccaaag agtatcttca
    10621 aaattccgaa aaaagaaaaa aaaacaccag aagtgagttc gtccgcgagt ggggcaccat
    10681 ataattcacg atcagctatc gaacccacta cggaacaaat taacgcgatt aacgaaattg
    10741 tatctgcggt ttcaagcgcg gaatttcgct ctttcctgct ttttggggta acgggttctg
    10801 gaaaaacaga agtctactta gcgtcagcag cgcgtgcctt ggaaatcgga aagtcggcgt
    10861 taattttggt tccggaaatt gcactgacac cacaattaac aagtcgcttc gagagtcgtt
    10921 ttggtgcgca ggttgccgtt ttacattcgg gactcactga aaaagagcgc cgcgaacagt
    10981 ggtggtccat tcgccgaggg gaaaaaaagg tagtggttgg tgcccgatcg gcgttgtttg
    11041 cgccgctgaa cgaccttggt ttgatcgtag tggacgaaga acatgagccg tcttttaaac
    11101 aagaagatcg cctgcgttat aacgccagag atttagcctt ggtacgagga aaaattgcct
    11161 cagcggcggt agttctcggt tccgcgacac cctccttgga aacgtttttt gcagctcaaa
    11221 acgggcgcca tcgattactg gagctaaaaa tcagaccgaa tcaacgccca atgccgtcca
    11281 ttgaaattat tgatatgaag ggaatcgacg gtcatttcag tgatccaatg cgaaacggta
    11341 tcgagcaagc gttaaaaaat ggcaatcaat gtatggtttt tgttaatcgc aaaggttttg
    11401 cccatttttt attgtgtact gaatgtggcg aagtaccaaa gtgtctaaac tgtagcgtgt
    11461 cactcactta ctatcgcgcc tcgagcactc ttcgttgtca ttactgtgac tttcagattc
    11521 cttcccattc cgcctgtaaa tcctgtaatt cgattagtct taaacaagtg gggttcggaa
    11581 ctgaggcgat tgaaaacgaa ttgaaagtta tatttcctgc ggccagcata cgcaggctgg
    11641 acgccgaaac agtcgataca aaaaagaaat tagatgaagt cttgaacgat tttcgctccg
    11701 gtcagataga tattttggtc ggcacccaaa tgttggctaa gggacatgat ttcccaaatg
    11761 ttacttttgt tgggatcgta ttggccgaca cggcgttaca tttgccagat tttcgtgcat
    11821 cggaaagaac atttcaactt gttacccaag tggcgggtcg ttgtgggcgt tccgataaac
    11881 cgggtaccgt ggttttgcag acgtttgtgc ccgatcatta tactattatt aatgccgcta
    11941 aacagagtta tcgagagttt tatgaaacgg aactcgccat tcgtcgcgac ttttcatacc
    12001 cgccatttag caaaatggcg caaatcgaat ttcgtcacct taaagagaat gtcgcgcgag
    12061 atcaggcatt catggctgag gccttactgc gaaatgctgg cgctgattca atgggcgtag
    12121 aatgtttagg gcctgctgcc gcttcgatcg cccgcattgc gaatcaatat cgctggcagt
    12181 tgttgctgaa agcggcaaaa tcttccgccc tgaacggtgc gatcaaaacc cttcgcaagg
    12241 ctggagtgag atttattgat gtcgatcctg taagcaccct ataatggttg aatgggtaag
    12301 attgagttct cttcagaacg cggtaagtgt cctgcctgtc gaactgaaaa tgtaattttg
    12361 aaagaagtta gtggaattca cgtttgcgaa aaatgcgcat ccaaacgatt ttctacgtcg
    12421 gtaagaaaaa gaatcgaaga agcctacaat cgtcagatgg acgccaagcg acgcgaagcg
    12481 tggatgcaac gcatggtggt cgcaaaacgc ggggtaaagt attacgaaga aggaaagttg
    12541 cttgagtcgt taaagtgctt tcgtgattac ataacgattc tcgaatcacg gtatcgagta
    12601 cccccgggcg gattgcaccc ctctctattt gacgttaaga aagatgccgg cgaactgttg
    12661 ctgatagccg gtgtttattg ggatatggtc aaggcatacg accatatcaa aggtcacacc
    12721 ggtgaaatgc gcggggcttt gaataaattt ttggaatttt cgatagatcg cccccacgta
    12781 atattagcct cggagaccat tcgaaaatac gtagcttccg gatccgcagt acacgtaaat
    12841 gatttcaaga gagctcatga acaattgcga agtaatcttt caaagtgttt tatcgccacg
    12901 gcagtttatg gacccaagtc cgacgaagtg gctgctttac gtgcgtttcg tgatcatttt
    12961 ctaaacaaga atcttatcgg aagaagtgtt gtcggttttt attacgcaat ttcaccgccg
    13021 cttgctcggt tggcggtgcg gcagccccgc attgccgacg ccattcggcc ctttttaaat
    13081 ctcatcgtga gaagcactaa aagagcgagc cgtctcagta cagataggta attttcgcag
    13141 ccgcgggagc tctggtggtt ctgcccaaac ccacgctatc tacgaggcac tattttccct
    13201 taaacatttc taaaagaagt tttaatggtc gagcgaatgt accgatatcc ttagaagagc
    13261 gatgtaaagc ttcaatattt gaatgaaaac tgaaaaaaat ttgaaagacc aaaaatcgca
    13321 attagaccag ctgattacaa ttctaatgag ttgttttgca gaacttggtg tacgcccaaa
    13381 ttcgcttttt attgaagaca ctgccgttat gatatacgac gcaatgtcga ctcgtacccg
    13441 attgtatcat cgactcgaac attgtctttc tactccacct aacgtcgacg cgttgggaat
    13501 attggcgtat ctttttcacg atgtcattta ttggcaggta gaccgagcta gaagtagcaa
    13561 actacgacaa ggtattgcac cgcttgaact cgatgaccac ggaaccgtta cgctcaccgg
    13621 tgaatgtgga gagttaggca ccgctaaaat aagtgaacgc aacacgctcg cccgactggt
    13681 attcggattc aatgaaccag ttgaactcga cttttcacat ggtatgaacg agtttctcag
    13741 cgcgtgtgta gccgaaaaca cctttaaggg cctgctggat acctggcaga ttgtacaaat
    13801 tctctgctgt attgaagcca cgattccatt tcgtccggca ctcgatggga aaaccgcaat
    13861 cgaaaagctc gcggccagat tgaaaaaaat taatattggt tatgcgtttg gtaaaacgga
    13921 agaagagcaa aaacaggcta ttcgacgtgc aatcgacgtg gcaaaccacg atgtggggaa
    13981 cttcgcgtat gccactacga gtacgttttt aaaaaacacc tgggatttaa cctgggaaaa
    14041 caaccccgca cttcagcgtg acacctactc tatcaagcaa taccggagtg cgctgcaagc
    14101 gaacatcgca ttttatcgaa gtctacaacc tcggtacgtt ttcagttgtc tcgacggtta
    14161 cccgacccgc ggacattttg aagacattgt ggggcgtact aaaaaaaata tcaccaccgg
    14221 cttacagtac attcaagtaa agctagttgc atgtgcaatt atcgaatccg ttgcagatct
    14281 tacaggtggg gacgcccccc tcatgttatt tatgggaaat cattcgcaac aggggctaat
    14341 tccaaaatgg gccgacttgc ttgaaaatat tgatcccacg ataataaaag acgagcttcg
    14401 aaaagatgtt tttaaattac ttttcgaagg tcgcccggaa atgaaagaac aatttgacgt
    14461 tgcccgctcg ccgatctcgg cacatctcta tgcatcgctc gacgctgcta attttcagaa
    14521 ggcggctgca ctagcagacg agtttttcgt tcagcgtatg agtgcaaaag aatttcttca
    14581 ggccaatccg attaatttag tttatggtcc ggtccttgaa aaatttaagg aaatcgtttc
    14641 tagccgaaaa gaagcgctta gcgctcttga aaaagagctg cg
//
LOCUS       DAOMXO010000003        12897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_2651_length_12897_cov_4.691870, whole genome shotgun sequence.
ACCESSION   DAOMXO010000003 DAOMXO010000000
VERSION     DAOMXO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 12897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12897
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2651_length_12897_cov_4.691870"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLH57_00155"
     CDS             <1..432
                     /locus_tag="PLH57_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016756 - glutathione
                     gamma-glutamylcysteinyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA];
                     GO_process: GO:0046938 - phytochelatin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytochelatin synthase family protein"
                     /protein_id="HRK01048.1"
                     /translation="VSEKGFRGKHGVPLVALENIVGEVFRVYGQSGLKIRRVHVSNTN
                     ASTLNKIKEDLVKNEISGRNFIIANFDGSKFFDDPEPWGHIAVIGAFDEDTNRILILD
                     PYRTWYSPYWVTLERFVLALATKDTENGNFRGYLYLEFPQS"
     gene            complement(436..861)
                     /gene="dut"
                     /locus_tag="PLH57_00160"
     CDS             complement(436..861)
                     /gene="dut"
                     /locus_tag="PLH57_00160"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004599922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRK01049.1"
                     /translation="MTTQLPVQKLNPDARLPGRAHPGDAGLDLFCIGDVTLNPHEPAK
                     VATGIAMAIPEGHVGLICDRSSMGAKGIRTLGGVVDAGYRGEVQVLLINLRNEPYKIS
                     RGDKIAQMLILPVNLIPTTEVKELSDTVRSSGGFGSTGR"
     gene            complement(889..1395)
                     /locus_tag="PLH57_00165"
     CDS             complement(889..1395)
                     /locus_tag="PLH57_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01050.1"
                     /translation="MIIFILGTNLAFAQNRKAPPSKFQPIQESVTSDGGTRANHFSGF
                     DFALGQPVPSYGGLMIGFHVHDDARLSLAIGEAGQWMTYALDTKIFLSPESVAMYVGG
                     GLSYMNGSEGKFLFWDLQFSNGLIPYIQGGVDWVNEEGFHLGANLGVTVPNGEVVVLP
                     GVAIGWYF"
     gene            1461..2339
                     /locus_tag="PLH57_00170"
     CDS             1461..2339
                     /locus_tag="PLH57_00170"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRK01051.1"
                     /translation="MRTIMVIGANGFLGSSIVNYFGKKHKVIALFNKHILRFPGTTHF
                     NYNLSDRDYMKRIISLTKPDTIIYCAGINDFVECAKNQRMAEAVNSYGPTLISSAADT
                     VQHRFIYLSTAFVYDGKRGNYLETDVVLPQSAFGKYKLAGENYVRSKFMTYTVMRLSP
                     VYGLGTIYHPSQFDQIRMKLQRGERVELPQNEMHSFLFVDIAMRGLEWVITQESRNRT
                     YNLGGLTKVTWFDFGKLIAESFGFNPDLVMPAKGNFEEDVDFSLNGTDLVRLSQVDPL
                     IMEQGLDLLKQQLIRR"
     gene            complement(2271..2870)
                     /locus_tag="PLH57_00175"
     CDS             complement(2271..2870)
                     /locus_tag="PLH57_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01052.1"
                     /translation="MATSKNVLSTELGWIHELAKNEINPDANSTINDMAGLDPKQLIE
                     ESTIGFLEDLRDLFTMFAKSFNGYSENNSRFSEAKIYGITNTPADFMVFRNNVKLVFS
                     NSAHGIINATFTQHTRNDLAADGSSTSQKQSHDIIAQMGPFMDVSWTYQGEKVNPERL
                     VKYFFIEFVKASRQLKKSSANQLLLKQIKALLHDQGIDL"
     gene            complement(2904..3078)
                     /gene="ssrS"
                     /locus_tag="PLH57_00180"
     ncRNA           complement(2904..3078)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLH57_00180"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3146..4291
                     /locus_tag="PLH57_00185"
     CDS             3146..4291
                     /locus_tag="PLH57_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK01053.1"
                     /translation="MGKKLLVADDSATIQKVIKLALSSEGYDILAVSDGREAVRAIQE
                     ERPDIVLIDVALSNGDAYTVKKAINQDSSLAGIRFILMASAFEKVDEKAASGARFDSR
                     LIKPFDPSHLRKMISDLLKSPATPTAAPPPPAEVPVGGAVTVELSTEDEAQAKEISDF
                     IAVTPPAPPQSVPEITVFDGMESTPSEVEAESDVGEEEDLEATQALTHEEARALENDI
                     RDLTESTIKMSEPDELEWSLNDDKKMRTAPVRSSRSETEASFDGEKTVELAKVDLTGR
                     RGIVSAPTKAFDDGGSNFLKIGLEQSARGSDATRTTNIMSPQSSTDESPSKNSNTSGT
                     LPFSQQELEVFVQKDIQEVIERLAKEMVPKIAENLIRKEIEKILSEP"
     gene            complement(4294..5046)
                     /locus_tag="PLH57_00190"
     CDS             complement(4294..5046)
                     /locus_tag="PLH57_00190"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRK01054.1"
                     /translation="MNNFYFKKMESCASTNDEAWKIPNTQWPALVSTDSQTNGRGRQG
                     RVWSTAPGLQILATLIFKPALPPEQITWIPVLAGICAHEAIAAIDLGLAERLRLKWPN
                     DLYVGTSKIGGILCESRFQGSELAGVVVGIGLNLASSPRIDRNTTSIAEMLEDSSFQI
                     SLHHFRNRFLNSWASLLVAAATPPIDRTYWSQKWNHYARLDSFFELDVRNSPQTMVRA
                     RVHELTEEGCLKVRILEGERAGELVFLTQVDS"
     gene            complement(5043..5924)
                     /gene="nadC"
                     /locus_tag="PLH57_00195"
     CDS             complement(5043..5924)
                     /gene="nadC"
                     /locus_tag="PLH57_00195"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRK01055.1"
                     /translation="MGDLKNLLPDLLNDSLVDNLIDLSLLEDGISNDLTTLATGCAEV
                     GRTVRATVIAKKTTIAAGGILSRKVLQRAGFSEKVTVSILADDGASLGDKAPWIILEG
                     SANAILRTERTILNFLMRMCGIASHTRQIVTLLEGTNCKLLHTRKTVPGHRLTDIYAT
                     LVGGAYPHRKSLSDAILVKENHLRASQSLQHLKDGIAKFRDQASFVEIEVSNFIELKY
                     AMENKPNRVMLDNFSVADVQKAVNLFGSAVELEASGNINLETVRAYAETGVDYVSMGA
                     ITHSSPAADLSLLFDFS"
     gene            5967..7160
                     /locus_tag="PLH57_00200"
     CDS             5967..7160
                     /locus_tag="PLH57_00200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRK01056.1"
                     /translation="MSDRISRSIDIARKAFYPVFFLVLTIPLYAQSKPRTDWLFKATD
                     DAKPKTLTVRRLTDGGDFISAKQFCFSLGCKLVYQWSASRLIISHRTEKRSAILNRFL
                     RHVLINGSVFKLGNPILGDAKDGYLLPMSLAVVLTRELKLGKIWSEVERQPITETPRA
                     GIREEELKRIVIDPGHGGADLGTSSGGILEKDVALIYAVRLQEYLRREIPDLEVSLTR
                     SGDEYVSLADRAKLANSKNAQLFLSLHLNHAADSKVEGVETYILSPDATDDDARKVAL
                     LENETWLKSTQLTGDKAGKTDLLKSILVDMEQTKFIQSSAMIAALINQELKSLEKTRG
                     LKSRGVKQAMFYVLSQVAMPSVLLEIGFLSSAGDRGRLMDVAFRDEFARSVVSAIKRF
                     DERKY"
     gene            7163..7882
                     /gene="rph"
                     /locus_tag="PLH57_00205"
     CDS             7163..7882
                     /gene="rph"
                     /locus_tag="PLH57_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRK01057.1"
                     /translation="MPTIERSFNRGSGDLRPVKITTGVNAYAEGSVMVEFGGTKVLVT
                     ASVEESVPAWLKGKGQGWITSEYGMLPRATHTRSKRERDKLSGRTHEIQRLIGRALRT
                     CIDLGRVGERTIMVDCDVIQADGGTRTASITGGYVALALAMKKLAATQAWNKTPIIEQ
                     VASVSVGLKNGEVLVDLDYKEDSSCEVDMNFVMTASGLFVEIQGTAEHKPFSQGDMQK
                     MTASAMEALAKLHQIQKDALL"
     gene            complement(8247..8558)
                     /locus_tag="PLH57_00210"
     CDS             complement(8247..8558)
                     /locus_tag="PLH57_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01058.1"
                     /translation="MGKQGAPEQKKPEGTEKKEAPKGAPHAGGAPSAAPMGGPSKGAG
                     AKIGCLAQGCKDKDSRFNFCEEHFRQFKFGLITKTGLPVMDYEKKLEHYQKWLRAQKV
                     A"
     gene            8720..10087
                     /locus_tag="PLH57_00215"
     CDS             8720..10087
                     /locus_tag="PLH57_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRK01059.1"
                     /translation="MARIGVVLFALMFISVHFAHAHSTGGFPGANFVHVKATTKKDRT
                     ELNNIGMSIDAVVSDAVYGTVNDATLELIKKSKFKIIETFKIDTGKAKISSFPPGDEP
                     YHDYAELSAALQALADKHPDFVKLLSIGRTVEGREIWCVRFNTSKEVMESDDAAELSG
                     KPGVLFVGNHHAREHLSAEVPLMLAQYLASNYGTDAGITELINTRDIMIVPMLNPDGV
                     EFDINKQPGRYQMHRKNMRDPRGQRSIDLNRNYGYKWGGDGASDNPNSETYRGPEAFS
                     EPETQALRDFVSVMPNLKMLLSFHTFSELILYPWGYTDDPICRDDCGDEKKRMDQLAL
                     VAMARQMARWNKYTAKQASGLYIVSGDTIDWAYGTYGILGYTFELSPKWAWGGSGFYP
                     GADIIQKVFEDNLNPALHMINLAADPYRSIETEWPGGVVSDEDLYGDYDPNDFNDDWL
                     FRMKR"
     gene            complement(10071..11768)
                     /locus_tag="PLH57_00220"
     CDS             complement(10071..11768)
                     /locus_tag="PLH57_00220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRK01060.1"
                     /translation="MLRLLLVLIVLLPVVPFLNSRVLRMAGDEKVYINQAVEMARSNS
                     WFFQTMADEPSYFKGPLHYIFIRIGMVLFGDSLAAGVWMNALALVIAALAMFKLAHRR
                     WGPRAGFLLGLATALNVGAVSHTFASQMEVELLALTALAISALGLAPEAFAQSGSKSR
                     VSFRSDLFFWLIAGIIGWVKSPLHSVLLGTSAIIYWAFTRQLWPRLKNPKAWACVFVG
                     MAVCVAGYLPAYLGDQEAFMATYLGREQFQKGGNNRAWHYPTLPLIHFLLPWTLIVFA
                     AVGKLLWRATSGKRRFMSMPNQFAIDRRMMLLGAAVSVPTILFWMFWDYKGQNYNLPI
                     LPGLLLFGFACFAKAHVEPKPPMLVYRIVSFLCLAALFAFIGLIAHFWPLPEWWNIYW
                     VYATFATMAVFIFCFAYGKTVKTLTIGTVAFFFAFALATTPLGNREMRDVRNYLKENP
                     KTKIYYYNLEPSIWSEWGLLQFTLNRPVFGLHRINQIQEATKPGHTVLVPNAKALATV
                     LAEWKKIHPSAEREPNIRTWKRWLTKGKSPSGEPMWKVAWTKRDLSHLERDFFIVSF"
     gene            complement(11768..>12897)
                     /locus_tag="PLH57_00225"
     CDS             complement(11768..>12897)
                     /locus_tag="PLH57_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01061.1"
                     /translation="TTFENNRKHRAFLLIAALILGTFSHVLFLAFAIAIFGIASLEGK
                     PSRFITVVYCTALVFILVRMTLDLRKIGPVFLAGGAGITVAFFLAKKETYFSRATLLA
                     RPLFEIVAIPFFLLFLFLMWGGPWSYAQATGSLVFWWWPLNIVLLIALYLNSGRWNWT
                     RDSTKFSWERRAWFGFVLSLIACNAMVYKPAPRYFVIPAIFLTIWAALAFDDYWQRSR
                     FQPRARTLLGLSLVFAFIVNLFVFQSDYLNRFLTRPGLDREFRLWHFNDSARDFRPFQ
                     RVFSWAEKNGCALTLDWVEDDRFGLPLKFLQLDSKPTGKACPWHKKQLFFSHIPNFNS
                     DLRIAERLRVGESIAHPQYGLRILAHEPEWGDLAIWIRDLP"
BASE COUNT         3594 a         3048 c         3078 g         3177 t
ORIGIN      
        1 gtttctgaaa aggggtttag agggaagcac ggcgttccat tggttgcttt ggaaaatata
       61 gtgggtgagg tgttcagggt ctacggtcaa agtggattaa aaattaggcg agtacatgtt
      121 tcgaatacga acgctagtac gctcaataaa attaaagaag atttagttaa aaatgagatt
      181 tctggtagaa attttattat cgcaaatttt gatggatcga agtttttcga cgaccccgaa
      241 ccttggggtc atattgcagt cattggtgct ttcgacgaag acacgaatcg aatcttaatt
      301 ttggatccct accgaacatg gtactcgcct tattgggtaa cattggagcg attcgtttta
      361 gcgttggcga cgaaggatac tgagaacgga aattttcggg gttacttgta tttggagttt
      421 ccgcagagct aattactacc tccccgtact cccaaagcca ccgctggatc ggacggtgtc
      481 ggaaagttct ttcacttcgg tagttgggat taagtttact ggcaaaatta acatttgggc
      541 gattttatca ccgcgtgaaa ttttgtaggg ttcatttcgc aggttaatca acaacacctg
      601 aacctcaccg cgatagccgg cgtccactac cccgccgagt gttcgaatgc ctttagcccc
      661 catcgaactg cgatcgcaga tcaatcctac atgcccttcg ggaatcgcca tggctattcc
      721 ggtggcaacc ttggccggct catgtgggtt gagcgttacg tcgccaatgc aaaataaatc
      781 taggcccgca tcaccagggt gcgcacgacc cggaagacgc gcgtcggggt tgagtttttg
      841 aacaggaagt tgagtcgtca tgattggttt ctccgtgtat tccgagtact aaaaatacca
      901 accaatggca acgccaggaa gtacaacaac ctcgccatta ggcacggtga cacctaggtt
      961 tgctccaaga tgaaacccct cttcgtttac ccagtcgacc cccccttgaa tataagggat
     1021 caggccattc gaaaactgta ggtcccaaaa tagaaacttt ccctctgatc cgttcatgta
     1081 ggaaagccca ccacccacgt acatagccac actttcgggt gatagaaaaa tttttgtatc
     1141 gagcgcatag gtcatccact gaccagcttc accaatggct aaagaaagcc tcgcgtcgtc
     1201 atgcacatga aatccgatca ttagtccgcc ataagaaggc accggttgac caagtgcgaa
     1261 atcgaatccg gaaaaatgat tggcccgtgt tccgccgtcg gacgtcacac tttcctgaat
     1321 gggctgaaat ttactcggtg gcgctttacg attttgcgca aaagcgaggt tggtacccaa
     1381 aataaaaatg atcattatcc cgagccgcgc tggcttactc atatgactca agtgtacaat
     1441 atttctgggg gaatgtattc gtgcgaacca tcatggtaat cggtgccaat ggctttttgg
     1501 gctcctcgat tgtaaattat ttcggaaaaa agcataaagt tatcgcacta ttcaataagc
     1561 acattcttcg atttccgggt accacccact ttaattacaa tctgagcgac cgcgactata
     1621 tgaagcgaat tatttcgctc actaaacccg acaccataat atactgcgct ggaatcaacg
     1681 acttcgtaga gtgtgcaaaa aatcaacgaa tggccgaggc cgtgaattcc tacggcccca
     1741 cacttatttc atcggctgct gacacggttc agcatcgatt tatctatcta tcaaccgctt
     1801 tcgtttacga tggcaaacgc ggcaactatc tagaaaccga cgtagtgctt ccccagagcg
     1861 ccttcggtaa gtacaagctt gctggagaaa actatgtacg aagtaagttt atgacatata
     1921 ccgtcatgcg actttctccg gtttacgggc tgggaaccat ttatcacccg agccaatttg
     1981 atcaaattag aatgaagctt caacgcggcg aacgggtaga actgccacaa aacgagatgc
     2041 attcatttct ctttgtcgat attgccatgc gggggctcga atgggtcatt acccaggaat
     2101 cgcgaaatcg aacctataat ctgggtggat taaccaaagt tacttggttc gattttggaa
     2161 aattaattgc cgagtcgttt ggttttaatc ccgatctagt catgcccgcg aaaggtaact
     2221 tcgaagagga cgtcgacttt tccctcaatg gcacagactt agtgcggttg tcacaggtcg
     2281 atcccttgat catggagcag ggccttgatc tgcttaagca gcaattgatt cgccgatgat
     2341 tttttcaatt gccgagaagc ctttacgaat tcgataaaga agtatttcac aaggcgttcg
     2401 gggttcacct tttcaccttg atacgtccag ctcacatcca taaaggggcc catttgagca
     2461 atgatatcgt ggctttgttt ttgagaggtt gacgaaccgt cggccgccag atcgttgcga
     2521 gtgtgttgag taaaagttgc gttgataatg ccgtgagctg aatttgaaaa caccagtttt
     2581 acgttgttac gaaaaaccat aaaatcagcc ggagtattgg tgatgccgta aattttggct
     2641 tcactgaagc gagaattatt ctcgctatac ccattgaaac tcttggcaaa cattgtgaac
     2701 agatcgcgca gatcttcgag aaacccgatt gtgctctcct cgatgagctg cttgggatca
     2761 agtcccgcca tatcatttat ggtgctattc gcatcagggt tgatttcgtt tttagcaagc
     2821 tcgtgaatcc aaccgagttc tgtcgaaaga acgttttttg aagtagccat ccgtaacctc
     2881 actcaaaaag ctggcaaaat ttagttgggc gcgaggcaag ggggagcctg tttgaaaccc
     2941 gcgactctga caggtgggcg ccgtttaacc gctttaggct tcctgattga acaggcttgc
     3001 tcaccacgat taggcacagc tcccttacga acgaattgta ggttcaaacc gtttacaccc
     3061 cgggcctcgc gtgcccagtt acgatcatag cacaggattt gacacgcttc atagcggggt
     3121 gggatactat ttaagatagg atgctatggg aaaaaaactc ctcgtcgccg atgacagcgc
     3181 cacaatacag aaagtaatca agctcgcgct ttcttcagaa ggctatgaca tattggctgt
     3241 ttctgatggg cgcgaagcag tgcgtgctat tcaagaggaa cggccagaca tcgtactgat
     3301 cgacgttgcg ctctcaaatg gcgatgctta caccgtaaaa aaggcaataa accaagattc
     3361 gtcgctcgcg ggaattcgct ttattttgat ggctagtgcc tttgaaaaag tagacgaaaa
     3421 ggcggcctct ggggcccgtt tcgattctcg tttaatcaaa ccctttgatc cctcgcattt
     3481 gcgcaaaatg atctctgatc ttttaaagtc gccggcgaca ccgaccgccg cgcccccccc
     3541 gcccgctgaa gtaccagtag gtggtgcggt cacagtggag ctttccaccg aagatgaagc
     3601 gcaggctaaa gaaattagtg attttattgc agtaacaccg ccggcgcccc cgcaatctgt
     3661 tcccgagata acggtattcg atggaatgga atctacccca agtgaagtag aagccgaaag
     3721 cgatgttggc gaggaagaag acctagaagc cacccaagct ttaactcacg aagaagcgcg
     3781 cgcacttgaa aatgacattc gggacctcac ggaatctact attaaaatgt cggaaccaga
     3841 cgagttggag tggagtctta acgatgacaa aaaaatgcgc accgctccag tccgtagttc
     3901 ccgtagcgaa acagaggcga gttttgacgg cgaaaaaacg gttgagctgg ctaaggttga
     3961 tctaactggt cgtcgcggaa tagtgtcggc accgaccaaa gcttttgatg atggcggtag
     4021 caattttttg aaaataggcc tggaacaaag tgctcgcggc tcagacgcca cgagaacgac
     4081 aaacataatg tcgccacaaa gttcgaccga cgaaagtcct agcaagaatt ccaataccag
     4141 tggcaccctg cccttttcgc aacaagagct cgaagtattt gttcaaaaag atattcagga
     4201 agttatagaa agacttgcta aggaaatggt gcccaaaatt gctgagaatc taattagaaa
     4261 agagatcgaa aagattcttt cggaaccata aatttaagaa tcaacttggg ttaaaaatac
     4321 taattccccc gcccgttccc cttctaaaat tcgcaccttc aggcaaccct cttctgtgag
     4381 ttcatgaacc cgtgcgcgca ccatggtttg ggggctattt cgaacgtcga gctcaaagaa
     4441 agagtccagg cgggcataat gattccactt ctggctccaa taagtccgat caatcggggg
     4501 tgtggctgcc gctactaaaa gcgaggccca tgaatttaga aatcgattgc gaaaatgatg
     4561 tagagaaatt tgaaacgaac tatcttctaa catttcagcg atcgaagtgg tgttgcggtc
     4621 aatccgaggg ctagaagcca aattaaggcc gattccgacc acaacccccg caagctccga
     4681 cccctgaaac cgtgactcac agagtatacc cccaattttg gaagtaccca catataggtc
     4741 attgggccat ttcaaccgca acctttcggc tagacctaag tcaatcgcgg caatggcttc
     4801 atgagcacaa attcctgcaa gtacgggaat ccatgtaatc tgttctggtg gtagcgccgg
     4861 cttgaaaatg agcgtggcca aaatttgcaa accaggggcg gttgaccaga cccgcccttg
     4921 cctgcctcgt ccattagtct gcgaatcagt gctaactaga gcgggccact gagtgttggg
     4981 gattttccag gcttcgtcgt tggtagaagc gcaagattcc atttttttga aataaaaatt
     5041 gttcatgaaa aatcaaaaag caaagataga tctgctgccg gagacgaatg agtgatggca
     5101 cccatggaaa cataatcaac ccctgtttcg gcgtatgcgc gaacagtttc gagattgatg
     5161 ttacccgaag cttccagctc caccgcacta ccaaaaaggt tcacggcctt ttgcacatcg
     5221 gcaacgctga aattgtcgag cattactcga ttaggcttat tttccatggc atatttgagt
     5281 tcaatgaaat tcgaaacttc aatttctaca aaagaggctt gatctcgaaa tttggctata
     5341 ccgtctttta aatgctgcaa tgactgggaa gcccgtaaat gattttcttt caccaaaatg
     5401 gcatcagata ggcttttgcg atgcggataa gcgcctccca cgagcgttgc ataaatatcg
     5461 gttaaacggt ggccgggaac cgtttttcga gtatgtagca gtttgcagtt agttccttct
     5521 aagagcgtaa cgatttggcg agtatgagaa gcaatgccac acatgcgcat aagaaaattt
     5581 aaaatggttc tttcagtacg taggattgcg ttggcagagc cttccaaaat tatccaaggt
     5641 gctttatcac caaggcttgc gccgtcgtcg gctagtatag acacagtgac tttttcggaa
     5701 aacccggctc gctgaaggac ttttcgagac agtatcccgc ctgcggcaat tgtggttttt
     5761 ttagcaatta cggtggcgcg cacagttcgg ccaacttcgg cgcaaccagt tgcaagggtg
     5821 gtgaggtcgt tagaaattcc gtcttcaagc aacgagagat caatgaggtt atccaccagt
     5881 gaatcattta ataaatctgg cagtaagttc ttcaaatcgc ccatggggca cttctatcgc
     5941 agtcagggtt aatgtaaagt aatgttatgt cggatcgcat tagtcgaagt atagatattg
     6001 cacgcaaggc gttttacccc gtatttttct tagtgctgac gatcccgctg tatgcccaat
     6061 ctaaaccgcg aacagattgg ctatttaaag caaccgatga cgccaagcca aagactttga
     6121 ccgtgcggcg actcaccgac ggcggtgact ttatttccgc caaacagttc tgcttttcct
     6181 tggggtgcaa gctagtttat cagtggtcgg cttcccgcct tattattagc catcggactg
     6241 aaaaacggag cgccattctt aaccgatttc tgcggcatgt attgattaat ggcagcgttt
     6301 ttaagttggg taaccccatc ttaggggatg ccaaagacgg ctatctattg ccaatgtcgc
     6361 tggcggtggt tttgacgcga gaacttaaat taggaaagat ttggtctgaa gtagagcgcc
     6421 agccgataac cgaaacaccg cgagccggaa ttcgggaaga agagctcaaa cgtatagtga
     6481 tagaccccgg tcacggcgga gccgaccttg gaacgtcgtc cggaggtatt ttggaaaagg
     6541 acgtcgcatt gatttatgct gttcgacttc aagagtactt gcgacgtgag attccagacc
     6601 tagaagtgtc tttaacgcgt tcgggcgatg aatatgtaag tctggccgat cgtgcaaagt
     6661 tagccaactc caagaatgcg cagttatttt taagcttaca tctaaatcac gcggcggact
     6721 ccaaggttga aggggtagag acttacattc ttagtcccga cgctaccgat gacgatgcca
     6781 gaaaggtagc gttgttagag aatgaaactt ggctcaagtc cacccaatta acgggcgaca
     6841 aggctggaaa aacggaccta ttgaagtcca ttctggtcga tatggaacaa acaaagttca
     6901 ttcaaagcag cgctatgatt gcagcgctca tcaaccaaga actgaaaagt ctcgaaaaaa
     6961 cacgaggact aaagagtcgc ggcgtgaaac aagctatgtt ttatgtgctt tcccaggtgg
     7021 caatgccctc ggttttgtta gaaataggtt ttttatcaag cgccggcgac cgtggccgat
     7081 tgatggacgt ggcatttcgc gacgaatttg ctagaagcgt ggtctcagcc attaaacgct
     7141 ttgacgagag gaagtattag ctatgccaac cattgaacgt tcttttaatc gcggttcggg
     7201 cgatttgcgg cccgttaaaa ttacaactgg ggtaaatgcc tatgccgaag gttcggtaat
     7261 ggttgagttt ggtggcacca aagtgcttgt cactgcttcg gtcgaagaaa gtgttcccgc
     7321 ttggttgaaa ggcaaaggtc aggggtggat cacctccgaa tatggcatgc ttccgcgtgc
     7381 gacgcacacg cgttccaaac gcgaacgcga taaactttct ggccgcaccc acgaaattca
     7441 acgcctcatt gggcgcgcgt tgcgtacttg catagatctg gggcgcgtcg gtgaacgtac
     7501 cattatggtc gactgtgacg tcatacaagc cgacggtgga actcgcacgg cgtcgatcac
     7561 gggcggatat gtcgccttgg ctcttgcaat gaaaaaattg gccgctactc aagcttggaa
     7621 taagacacca ataattgaac aggtagcctc ggtgagtgtg ggtttgaaga acggcgaagt
     7681 cttggtggat ctagattata aagaagactc cagctgcgaa gtggacatga acttcgtaat
     7741 gactgccagc ggtttatttg tagaaattca gggaaccgca gagcataagc cattttcgca
     7801 aggtgatatg caaaaaatga ctgcgtcggc tatggaagcc ttggccaaat tgcatcaaat
     7861 tcaaaaagac gcgcttctct aacggagcga tttttaggat aaccccgaaa gttttacgcc
     7921 cgccccggcc agcgcatctg ttgtggcaac cgaagctaag ccaggtactt ttcgaagaaa
     7981 tattttaagt ggcaattagt tgaaagaaaa taaaaaatca ggagcctcgt cgatcgacga
     8041 ggcccctgat ctcactgcac ccaagtcaac atcagttcgc tgtaccgttt ttccaccacc
     8101 gagcgtttat cccccgctac cgtttgctct tttttcttca tgtcgccatg aaagaaatga
     8161 agagcaccct ggggtctgaa ctcccggcga cccgggagaa gtttccccaa ggtgctcgga
     8221 ggtgggtggg tacagctctg ctgccgctag gcaacctttt gagcacgtaa ccatttctgg
     8281 tagtgctcga gctttttttc gtaatccata accggcagtc cggttttggt gatcaaccca
     8341 aacttaaatt gtcgaaaatg ttcttcacaa aagttgaagc gcgaatcttt gtctttgcag
     8401 ccttgtgcga ggcaaccgat cttggcacct gcgccttttg acgggcctcc cattggggca
     8461 gcgctcggag cgccacctgc atgcggagcg cctttcggtg cttctttttt ttcggtgcct
     8521 tccggcttct tttgctctgg agcgccttgt ttacccattt ttagtgaccc ccgttacaca
     8581 ttgcctatcg gccaaaacag ctataacttt agtaattttg tcggatttta tgagcacggg
     8641 acaaaaaccc ttgtagtttc tgtagtttgt cttacgcttg ggttaccaac aattcatctc
     8701 catggaaggg agttctgcaa tggcaaggat cggcgttgtc ctgttcgctt tgatgtttat
     8761 ctcggtacat tttgcccacg cccacagcac tggcgggttt ccaggcgcaa acttcgtgca
     8821 cgtcaaggcg acaacaaaaa aagaccgtac ggaactaaat aatatcggta tgagtattga
     8881 tgccgtggtg agcgacgcag tatatggcac cgtcaacgac gcaacgctcg agttgattaa
     8941 aaaatctaaa ttcaaaatca ttgaaacgtt taagatcgac accggcaaag cgaagatttc
     9001 cagctttcca ccaggagatg aaccttatca cgactacgcc gaactatctg cggcgctaca
     9061 agcgttggcc gacaagcacc cagattttgt gaagctatta agtattggcc gaactgtgga
     9121 aggtcgcgaa atttggtgtg tgcggtttaa tacaagcaag gaagtcatgg aatctgatga
     9181 cgccgctgag ctttcgggca aacccggtgt gctgttcgtg ggcaaccacc atgcacgtga
     9241 acacttgagc gcggaagttc cgctaatgct cgcacagtac ctcgcatcta actatggcac
     9301 cgatgcggga atcactgaac ttatcaacac tcgggatatc atgattgttc caatgctcaa
     9361 ccccgacgga gtcgaatttg atatcaacaa gcaaccaggt cgttatcaaa tgcatcgcaa
     9421 aaatatgcgc gatccacgcg gtcaacgaag catcgacctc aaccgtaact atggttacaa
     9481 atggggcgga gacggtgcga gcgacaatcc gaattccgaa acctatcgtg gacccgaagc
     9541 atttagcgaa cccgaaactc aagcattacg agatttcgta tctgtgatgc cgaatctaaa
     9601 aatgctgctt tcatttcaca cttttagtga gctcattctt tatccctggg gttacactga
     9661 tgatccgatt tgtcgcgacg actgcggcga cgaaaaaaaa cgtatggatc aacttgctct
     9721 agtagctatg gcccgccaaa tggctcgttg gaataaatac actgcaaaac aagctagcgg
     9781 actatacatt gtgagcggtg atactatcga ctgggcatat ggaacttacg gaatcctggg
     9841 ttatacgttc gagctaagtc caaaatgggc atggggcgga agcggttttt atccaggcgc
     9901 cgacattatt caaaaagtgt ttgaagacaa tctgaaccca gcgcttcata tgatcaacct
     9961 tgcggccgat ccctaccgct caattgaaac tgagtggccg ggtggcgtag tttccgacga
    10021 agatctctac ggagactatg atccaaacga tttcaatgac gactggctct ttagaatgaa
    10081 acgataaaaa aatcgcgttc tagatgcgag agatctcgtt tcgtccaagc gaccttccac
    10141 attggctcgc cagagggaga cttccctttg gtgagccagc gtttccaggt tcgtatgttg
    10201 ggctctcttt cggcagacgg atggatcttt ttccattcag ccagcactgt ggcgagcgcc
    10261 ttggcatttg gtactaatac agtgtgcccc ggcttcgtcg cctcttgaat ttgatttatc
    10321 cgatgaagtc cgaataccgg tcgatttaga gtaaactgca acaatcccca ctctgaccaa
    10381 atcgaaggct ccagattgta gtaatagatt ttagtttttg gattttcttt tagataattg
    10441 cgaacatcgc gcatttcccg atttccaagc ggcgtggtcg ccaatgcgaa agcaaagaaa
    10501 aaagctaccg ttccaatggt aagggtttta acagtttttc cataagcgaa acaaaatatg
    10561 aaaacagcca tagtcgcaaa agttgcgtaa acccaataaa tattccacca ctcaggaagt
    10621 ggccaaaaat gcgcgatcag tccgataaaa gcaaataaag cggcaaggca gagaaaggag
    10681 acaattctat aaaccagcat cgggggtttg ggctcgacgt gggcctttgc gaaacaagca
    10741 aaaccaaaga gtaaaagtcc cggcagaatc ggaaggttat agttctgacc tttgtaatcc
    10801 caaaacatcc aaaacaaaat agtcggaaca gaaacggcag cgccaagcaa catcatccgc
    10861 cgatcgatag cgaactgatt tggcatgctc ataaaccgtc gcttgcctga agtcgcacgc
    10921 cagagaagtt ttcccactgc agcgaatact atgagagtcc atggcaataa aaaatgtatc
    10981 aacgggagag ttgggtaatg ccacgcgcga ttattgccac ccttttgaaa ttgttctcta
    11041 ccaaggtagg tcgccatgaa agcctcttgg tcaccaagat aagccggtag ataacccgca
    11101 acacaaacag ccattcccac gaagacgcag gcccatgctt taggattctt cagtcttggc
    11161 catagctgac gagtaaatgc ccaatatatg atcgcactcg tgccgagtaa aactgaatgt
    11221 agtgggctct ttacccaacc aatgattccg gcgattaacc aaaaaaataa atctgaacga
    11281 aaagaaacgc gactcttact acccgactga gcaaatgcct ctggggccaa acccaatgcg
    11341 ctaatagcca aagcagtgag ggcaagcagt tcgacttcca tttgactagc aaaagtatgc
    11401 gaaaccgcac caacatttag ggctgtggcg agacctaata aaaaacccgc gcggggcccc
    11461 catcgccgat gggccaactt gaacattgcc aatgccgcaa ttaccaaggc tagtgcattc
    11521 atccaaacac cggcggccaa tgaatcacca aaaaggacca ttccaattcg aataaaaatg
    11581 tagtgtagcg gccctttgaa gtaactcggt tcatctgcca tggtttgaaa aaaccacgaa
    11641 ttcgacctag ccatttcgac agcctgatta atgtatacct tttcgtcgcc ggccattcga
    11701 agtacgcggg aattcaaaaa cggcacaacc ggcaacaaaa ctatgagaac caacaataat
    11761 ctcaacacta tggaagatcc cttatccaga tcgcgaggtc gccccattcg ggctcgtggg
    11821 ccaaaatcct cagcccgtac tgtgggtgtg cgatcgactc acccacgcga agtcgttctg
    11881 ctatgcgcag atccgagtta aagttcggaa tgtgcgaaaa aaataactgc tttttatgcc
    11941 aaggacaagc tttaccggtg ggtttagaat cgagttgtaa aaatttgagt ggtagcccaa
    12001 agcggtcgtc ttcgacccaa tccaaagtta gggcgcagcc gtttttttct gcccaactaa
    12061 aaacccgttg aaagggccga aagtcacgcg cactgtcatt aaaatgccaa agtctaaatt
    12121 cccgatcaag gcccggccga gttagaaaac ggtttagata gtcgctttga aaaacaaaca
    12181 aattaacaat aaacgcgaaa actaaactta gtccaagtaa agtccgtgcg cgcggctgaa
    12241 accgggacct ttgccaataa tcatcgaaag caagagccgc ccaaatggtt aagaaaattg
    12301 cgggaataac aaaatagcga ggcgccggtt tatataccat ggcattgcac gcgattagcg
    12361 acaatacaaa cccaaaccat gcgcggcgct cccacgaaaa ttttgttgaa tcccgagtcc
    12421 aattccagcg acccgaattt aaatagagag caattaacag aacaatgttt aagggccacc
    12481 accaaaaaac aaggcttccg gtagcctgtg cgtatgacca gggaccgccc cacattaaaa
    12541 ataaaaataa taaaaaaaat gggattgcca caatttcgaa caagggtctg gctagaaggg
    12601 tggctcgtga aaagtaagtt tcttttttag ccaaaaaaaa ggcgactgta atgccagctc
    12661 caccggctaa gaacacagga ccaattttcc gtaagtccaa ggtcatgcga accagaatga
    12721 aaaccaatgc tgtgcagtaa accacagtta taaacctaga aggtttacct tctaaactcg
    12781 cgatcccgaa tatcgcaatg gcgaacgcca gaaataatac atggctaaat gttcctagaa
    12841 taagtgcagc gattagcagg aaagcgcggt gtttgcggtt attttcgaaa gttgtca
//
LOCUS       DAOMXO010000004        12658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_2708_length_12658_cov_4.964532, whole genome shotgun sequence.
ACCESSION   DAOMXO010000004 DAOMXO010000000
VERSION     DAOMXO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 12658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12658
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2708_length_12658_cov_4.964532"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLH57_00230"
     CDS             complement(<1..588)
                     /locus_tag="PLH57_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRK01062.1"
                     /translation="MVLKNVAEKNQEESLELKNQFTQRQFLSELFFSNASFGLNLCRM
                     DGTWLESNPAFLKMVGYSRSEADGGLTYWQLTPQKYNDQERVQLENLARFGCYGPYEK
                     EFIRKDGSLIAVRLNGFIVEKNGEKFIWSIIEDLSKVRQIEDEERQLRIRYECLFNAV
                     DQTAIVAITDKQGKINYVNDNFCKISGYSRSEIQGK"
     gene            777..1391
                     /locus_tag="PLH57_00235"
     CDS             777..1391
                     /locus_tag="PLH57_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRK01063.1"
                     /translation="MTISLIGSLRSPYVRRIRAALVEMNLDFNFEVVDVWDPSPQFLA
                     ISPLERVPVFIFSGEHPIYDSHSIQAWLSVKYEDHPLFQGSLEGLSRRLHFTSLATGI
                     IDLAVQHFLEHRRGPAHSDMATIHDCERALKQAYAVFERELANPRPYLSGDSLRIEDI
                     DLGVAVFYTDFRVGTWLVDLFPNVRNFSDTLAKRKSFSETRPPI"
     gene            complement(1396..2298)
                     /locus_tag="PLH57_00240"
     CDS             complement(1396..2298)
                     /locus_tag="PLH57_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01064.1"
                     /translation="MKNKTVFAIWCAVFLALGTATFPCFAGNISDENPDLETILLRAP
                     HLRTSAEHAAFRFEELVSAPSSPWSSLSENELKAEREAVIKAFLWIETHMQLAAAKKM
                     NLVSLGRTIWGELLRELKPLLIKQQSGEWSVDDLAKVRALMHQALALSFLPNFTAVLP
                     MELPPKKMGPELARYVRLALSPLRYLYPGRLPSDSTVNAPSISKHMWATNILGGLPWG
                     YLAEHFDIIPHGTVLGLFASHLVLTAATTSAVLGHGIVELAKAPFTWPSARAAERLYG
                     DMRHVEKNASNLLCTAVILGRRPY"
     gene            complement(2332..3096)
                     /locus_tag="PLH57_00245"
     CDS             complement(2332..3096)
                     /locus_tag="PLH57_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRK01065.1"
                     /translation="MAKRHPEHVNLERWLVSYADFITLLFAFFVVMYAISQSDLAKLK
                     SVSESIREAFNAEAIDQQGKSSGKTLNQFDPASAQGGLLLDMPAGKSNIMAPDYEDLK
                     RVADILEESVSYELGDADIAEKMQIIYDDRGLVVRLSAKNFFEPGAADVKPEAVPILD
                     QMAKVLKTAKRHIRVEGHTDDLAVKSDYYPSNWELSTARAAWVVRYLIQKYKFDPRYL
                     TVAGYAEYHPIAPNKSEEGRAKNRRIEIVVLSKEPR"
     gene            complement(3097..3879)
                     /locus_tag="PLH57_00250"
     CDS             complement(3097..3879)
                     /locus_tag="PLH57_00250"
                     /inference="COORDINATES: protein motif:HMM:NF006583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HRK01066.1"
                     /translation="MEITTVVGVVLGLLAVFGGAVLEGLHIGSLLQGTAAIIVFGGTI
                     AAGLVSFPVQDVKNAIKMMNIVFQKVDTDVREVIDEIIKVATVARKEGVLALEGMRDS
                     INNPLFKKSVKFVIDGFEPQTVKDIMQSEIDIAYEEEEAAGKVWEGTGGYAPTIGIIG
                     AVLGLIHVMQSLDDPSKIGSGIAVAFVATVYGVGAANLLFIPFGTKLKRKAHMRAQVK
                     EVVKLGVIGIQEGLNPHFLREKLEVFVEEHLRGHGGAEGGEK"
     gene            complement(3858..4106)
                     /locus_tag="PLH57_00255"
     CDS             complement(3858..4106)
                     /locus_tag="PLH57_00255"
                     /inference="COORDINATES: protein motif:HMM:NF018043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HRK01067.1"
                     /translation="MIKVHRLNGKEFIVNCELIKYLEATPDTILTLVDNEKFMVRESI
                     DEIVNATRAYKRAIHAFSDAVESTNPLTSEQAWKSPQS"
     gene            complement(4145..6877)
                     /locus_tag="PLH57_00260"
     CDS             complement(4145..6877)
                     /locus_tag="PLH57_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01068.1"
                     /translation="MASYDEKDYEPILLQSIRADVVVPFDCYLLLRENDRIVHYIQSG
                     QVFPSEKLEKLKNFKHNTLYIHVAGKPAYVAYLESFFNTGDGLAVLKELNANNPGGKI
                     LGIPTEIDVAKIVASEVAKDVAGQPVNETVKVVPAGESVPLGEVAVVEQVTDHQKDEV
                     QKLAGKKQSVLEQVVAALKEDIDQLDQEVHASGALSEEQSTLIKSVTDRIDEELQKVE
                     GQPDEPETKTKVESVTEHIKEELARVRSLAEKSEKTLGRTLISAADDAEEELAKMLEN
                     PAKHKAALQRKMMRLKHLVNPAIEQDADEFAAEGVSRMVRKVEKLIGVKLAIQPTSES
                     GSVVSVKENSAAPSGKSDETKKAEGSATPAAGAAKQDAGTVPKTAFSNESEMVALIVQ
                     QAETIKNLEAKNNFAGKALADVLDKWNDFNAGSRKVFGPDQVNAANRFQLHLDTLKNA
                     YNDTNQQTGVLLRLSETLSGEMKGIKTDGLKKASSEPVATNSQVQSQATAVSSDDADK
                     NKKAQLDVPEQKTESSNIDEVRMENELLRAQLLNAKSLLEKSEEKILELSKMHEENDK
                     YISALEKEGVEHKELVKKAQDQVATIEKENIRHGEHSTELEKQLQIKKAEEEAKDLTL
                     NELRLKMLSLETNIRVFTDAFKAEGLDPAVMQEKDPKKLPFKPEAVAMVVEKDKMVVR
                     LSEQLTQKADEVKEARREIAKLRGLIQGTNLLKKEMSSKVDRINAERETIVKSQEGMQ
                     RKIMVLQNLAESERKTVSKLNSIIEELRKDRVEHMGKTAAALKDYKEVMSKAMSLNNH
                     VQAEVQRNKALNEQHQSLVQKQKELASENVQLQKQLKNLEVEYKRVQKLYKDASGSDS
                     TADKSMFDKRIADLREENKKIQTMYATEQRKSQQLEKELKALQFKLKNPKPSAA"
     gene            7017..8216
                     /locus_tag="PLH57_00265"
     CDS             7017..8216
                     /locus_tag="PLH57_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01069.1"
                     /translation="MNSSQSTNVHVSGRGLGARRIPIWITATRMLGAALFFCATLATG
                     LSILQWGWIQFGYTSGLAFKFALLTTTGALVFFLVVRNNSLSSLWNLRRIDRKWVYGG
                     HAGLTGMIASILIIAAPTTTLFWSLSEIINTFPTPTKLDVIKENPALDSTLKPVFLWE
                     YATVFDPRWFREFEIVLPYEKGPPQKLLGSFYLQHRKTRALGAAFYGKRPRQYDRSTV
                     AALFSERAATYPHPRHWPLLYNLAREAGALGANRLSLFETPYASRIGTSERLFFWSPA
                     GAVFQARCDSPCVFEPMLSHVRFAEDSKASLKSRQDLTKAKLKMLLETYPKNAEQRVL
                     HESELVLTLISWLTLDPRDPEAFFHIGKLATNRETVASAVRYGKDLKMSAPQIAELET
                     RLATFEY"
     gene            8367..9296
                     /locus_tag="PLH57_00270"
     CDS             8367..9296
                     /locus_tag="PLH57_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01070.1"
                     /translation="MGLLRFIKNRGAGTFGRISLLLTGAALAAGCPQQTATTKAPAGS
                     ISGVVSSSASSSSSATSANSSSVTAIQIITKADSSGSFDSAIVPVSGGVSLAASRLYD
                     LSGALIPSGSIPSWFVEARLFLTSTRTSAGAPTNTTSNTPCAYFDSYSDNNPDSSGYY
                     TIDGYNVTQTGILADIDQCAGTAAAELNQLGFYVRVDRRFMSSNDKLQVIVKAKALEE
                     PNTAPTSSSCVVGGIFNASNCSNQVYTLTRRTSPGAPAKPFINLFPSAKAWDLLAEDV
                     LIPLSDDTNISTISIDRVKGGAVFYGITIIKMQ"
     gene            9312..12203
                     /locus_tag="PLH57_00275"
     CDS             9312..12203
                     /locus_tag="PLH57_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01071.1"
                     /translation="MNKPWVARKIKSRGATFTALVCVGSALLFSGCPELDKATNLNTN
                     KTAVASSTASGSSGSVPIHYSEYQNAKSVKVVFNKQSFYNNFLTNSSGCAGDARLYYD
                     PVTDRQVPTKPSWLRNVAVELTNSNALDGPSYATGCSLVGSGSPTAANCATFDDLQPL
                     SGAASRAIVVGGMGCASNTSGCSALANSPTLKGDIWQAYIPNDADDMTWAKQNPALSV
                     FGVAASPGLAWAGGDFDELHNEFYIFGGVNAPDNTSTNLEFSNSVVKLSFNTDKSVSN
                     TTPPATVTTSTRRNFGGWALDGAFNVALAAYFTPPSLVGNTFTYSSRRDPTSKSWCLD
                     GDAQCSATAATESPVQVNNEHSDYFLLTGGIFDDDGVTAPFDDDYFTSKHFWMYKPHG
                     ISNSAGATAPSGGDWILLSNDEEASAALGNIMKVIEISNTGVAEDFWSASNATWNGRA
                     FHRTVYDPTMNRFYIFGGIEASANATTTIPTLGDNDIWIYDPPALGRRPTAGCYVAET
                     PNNVAIPTTSATLGIGTSSINVNRAYLGEKWVFPPGGCLQRIQHTTAPDARFEQAMAF
                     DREQKAVVVFGGCESPSVIGAEGSSTAGDPTSNCVSSTALFNDTWLYLPPTTMEFVSN
                     SYQDAGTSPYSVDGTSAPYYRLSNIFGTDFWLDYFPMYQDNNNSSVELTKPTDDQILG
                     TWIQMNPTTSPTKRSSASFYYDRAHKKFYLHGGFGCRNTSCSDVGTINDLWEYTPPNL
                     LTQCDRESGTCSSNGTWTQIKSHNDAAADQPTARKGAVTAHAEPMFSFADDFYTVTDT
                     SCVSQGPVITGDSSSNKQYVGAIYIDIDREKIESGRHLVINLRFLPFDQNTKLPGLFN
                     NNTPTYTSDDSENASINDQALIRVQLINNPVNFWDQIQSLIQPRFHEFLNNVPVISRD
                     LTYVAGGTGQVTEKQILIPLNGVDTSFDMIKIERIQGSVKFYEMAIYQY"
     gene            12392..>12658
                     /locus_tag="PLH57_00280"
     CDS             12392..>12658
                     /locus_tag="PLH57_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01072.1"
                     /translation="MSRIAVVLAVSFSFFYAHTLVAKEPATKKVASRKLASIKQLRPT
                     LNQTEAMLQSPHAAPRLLDKEPFGSAKHSRLGFYGVCRDKHRRSP"
BASE COUNT         3000 a         3363 c         2802 g         3493 t
ORIGIN      
        1 cttcccttgt atttcggacc gtgagtaacc gctgatctta caaaaattgt cgttaacgta
       61 attaattttt ccttgtttat cggtgatcgc gacaatcgca gtttgatcga ccgcattaaa
      121 taggcactcg taacgaatcc gtaattgtcg ttcttcgtct tcgatttgtc ttactttcga
      181 gagatcttcg attatcgacc aaataaattt ttcaccattt ttttctacta taaacccgtt
      241 aaggcgaacg gcgatcagcg aaccatcttt gcgaataaat tctttttcat aaggaccgta
      301 acatccgaaa cgagccaaat tctcaagctg gactcgttcc tggtcattgt atttttgtgg
      361 tgtcagctgc cagtaggtca gtcccccgtc ggcctcgctt cgagaatagc caaccatttt
      421 taagaatgca ggattagatt ccaaccacgt tccatccatt cggcaaaggt tcaaaccaaa
      481 tgaggcgtta ctaaaaaata attccgataa aaattgtctc tgagtgaatt gatttttcaa
      541 ttccaaagac tcttcttggt ttttctcagc aacgttcttc aataccattc ggaccccctc
      601 atcgcaatat tcagagatta gaatagcgca aaaaaccact cgtgcgactg gaaatctttg
      661 acgcagtcgc ttgaacctcc tcatgccacc ccgggggtga gatcgtggcg cgctctagaa
      721 aatggatttg ttaaatacgt tctagttaac tcaacggacc tttgttaaaa aaaattatga
      781 caatatcact gattggatca ctgcgatccc cctatgtccg ccgaattcgc gctgcattag
      841 ttgagatgaa tttagatttt aattttgaag tagtggatgt ctgggatcct tctcctcagt
      901 tcctcgcaat atctcctctt gaacgggtgc cggtttttat tttttccggt gaacacccaa
      961 tttacgactc gcattcgatt caagcatggc tttccgttaa atatgaagac catccccttt
     1021 tccaagggtc gctcgaggga ttgtctcgcc gtctgcattt cacttcccta gctactggaa
     1081 tcattgattt ggccgttcaa cactttttag aacacagacg gggtccagct cattccgaca
     1141 tggccaccat tcacgactgt gaacgcgccc tgaaacaagc gtatgccgta ttcgagcgtg
     1201 aactcgcaaa cccgcggcct tacctcagcg gagactcact aagaattgaa gacatcgatc
     1261 tcggtgtcgc ggtcttttac accgacttta gagttggaac ctggttagta gatcttttcc
     1321 cgaatgtgcg caacttcagc gacacgcttg ctaaacgaaa atcgttttca gaaactcgac
     1381 cgccaatttg accatttagt atggccgtct gcctaagatc acagccgtac acaaaaggtt
     1441 cgatgcattt ttttctacgt ggcgcatgtc tccgtaaagt ctttctgctg cacgtgccga
     1501 aggccaagtg aagggcgcct tcgccagctc gactatccca tgccctaaca ccgcactggt
     1561 tgtggccgcg gttaacacca ggtgtgaggc aaacaacccc aggaccgttc cgtgtggaat
     1621 aatatcgaaa tgttcggcca agtagcccca tggaaggcca cctaaaatat tggtcgccca
     1681 catgtgcttc gaaatactcg gagcgttgac cgtactgtcg ctcggaagtc gccctggata
     1741 aaggtaccta agcggcgata gcgccaaacg cacatacctt gccagctctg gtcccatttt
     1801 tttgggcggc agctccatcg gaagaactgc cgtgaagttt ggcaaaaaac ttagcgcgag
     1861 ggcttggtgc atgagcgcac gcactttcgc caaatcatcc actgaccatt ccccgctctg
     1921 ctgttttatt agcaagggtt tgagttctcg caacaactca ccccatattg ttcttccaag
     1981 actaaccaga ttcatttttt ttgcagcagc caactgcatg tgagtttcga tccacaaaaa
     2041 cgcttttatc acggcttcgc gttcagcctt aagttcattc tcagacaatg aactccaggg
     2101 cgagctggga gccgacacta attcttcgaa ccgaaaagcc gcatgctctg cggaagttcg
     2161 taagtgcggc gcccgcaata gaatagtttc aagatccgga ttttcatctg aaatattgcc
     2221 agcaaaacaa ggaaaagtgg cggttcccaa agccaagaac acagcacacc agattgcaaa
     2281 aacagttttg ttcttcatgc cagcacgcct agcacgaact tagaaagact actagcgtgg
     2341 ctctttgctc aagaccacaa tctcaatgcg ccgattcttg gcgcgccctt cttcggactt
     2401 gttcggcgca atcgggtgat attcggcata accagcaacg gttaagtatc tcggatcaaa
     2461 cttgtacttt tgaatcaagt accgcactac ccaagctgcg cgagctgtgg agagttccca
     2521 atttgacgga taataatcac tttttaccgc caagtcgtct gtatggcctt caactcgaat
     2581 gtggcgtttt gcggttttta agaccttcgc catttgatcg agaattggca cggcctcggg
     2641 tttcacatca gcggccccgg gttcaaaaaa gtttttcgca gagagtcgaa ccacgagtcc
     2701 gcggtcgtca tatataatct gcattttttc cgcgatgtcg gcatcgccca gctcataact
     2761 tacgctttct tctaaaatat ccgccactcg tttcaaatcc tcgtagtcgg gcgccatgat
     2821 attggactta ccggccggca tgtccagtaa caaaccaccc tgagcactgg cagggtcgaa
     2881 ttggtttagc gttttaccgc tcgattttcc ttgctggtcg atcgcttcgg cattgaaggc
     2941 ttcacgaata ctctcggata cagattttag tttagctaaa tcggactggg atatcgcgta
     3001 catcaccacg aaaaatgcga aaagtagcgt gataaagtcg gcgtacgaca ctagccagcg
     3061 ctctagattg acgtgttccg ggtgccgttt ggccatttat ttttctccgc cttcggctcc
     3121 gccatggcct cgcagatgtt cttcaacgaa tacttccagt ttttcgcgca agaaatgggg
     3181 atttaaaccc tcttgaatac caataacgcc caattttacg acttctttta cttgggcccg
     3241 catgtgggct ttgcgtttta acttggttcc gaacggaata aacaacaaat tggccgcacc
     3301 cactccataa acggtcgcaa caaacgcgac cgcgataccg ctaccaatct ttgacgggtc
     3361 gtcgagtgac tgcatcacat ggatcagccc tagaaccgct ccgataattc cgatggtggg
     3421 cgcgtatccg ccagtacctt cccacacttt tcccgcggct tcttcctctt cataagcgat
     3481 gtcgatttcc gactgcataa tgtcttttac ggtctgcggc tcaaaaccgt cgattacaaa
     3541 cttaacgctc ttcttgaaga gcgggttatt tatgctgtcg cgcatacctt ccaatgccaa
     3601 cacgccttct ttacgagcga cagtcgccac tttaatgatt tcgtcgatca cttcccgtac
     3661 gtcggtgtcg actttttgga acacgatatt catcattttt atcgcatttt taacgtcttg
     3721 caccggaaac gaaacaagac ccgccgctat cgtgccaccg aacacgataa tcgcggcagt
     3781 cccctgcaat aagctaccaa tgtgcagtcc ttccagaacc gcaccgccaa aaaccgccaa
     3841 aaggcctaag acaacgccta cgactgtggt gatttccatg cttgctccga tgtgagtgga
     3901 ttagtcgact ctactgcgtc tgaaaatgcg tgaatcgcac gcttataggc acgagtggcg
     3961 tttacaattt catcgatcga ctcgcgcacc atgaactttt cattatcgac caacgttaga
     4021 atggtgtcgg gcgtcgcctc cagatacttg ataagttcgc agtttacaat aaactctttc
     4081 ccgttgaggc gatgcacttt gatcatcacc tttgagttta gcagcgagtc gggcaatgta
     4141 aagcttaggc tgcggaaggt tttggatttt taagtttaaa ttgaagcgct ttaagttctt
     4201 tttcgagctg ctgagacttg cgctgctccg tggcatacat agtctgaatt ttcttattct
     4261 cttcgcgtaa atctgcaatg cgtttatcaa acatactttt atcggcagtc gaatcactac
     4321 cgctggcatc tttataaagt ttttgcaccc tcttgtattc tacttccaga tttttgagtt
     4381 gtttctgaag ctgtacgttt tccgaagcta gctctttttg cttttgcacg agcgactgat
     4441 gctgttcgtt caacgccttg ttccgctgga cttccgcttg aacgtgatta tttaagctca
     4501 tggccttact catcacttct ttatagtctt tcaatgccgc agcggtcttc cccatgtgct
     4561 caacgcggtc tttacgaagt tcttcgatta tagagttgag cttcgataca gttttacgct
     4621 cactctcggc cagattttgg aggaccataa tttttcgttg cataccctct tgcgatttaa
     4681 caatagtttc acgttcagca ttgatacgat caactttcga gctcatttct tttttcaaca
     4741 aattggtgcc ctgaattaat ccgcgtaact tcgcaatctc tcgccgcgct tcttttactt
     4801 cgtctgcttt ttgcgtaagc tgctcggata atcgaactac cattttgtct ttttctacca
     4861 ccatcgccac tgcctcaggt ttaaaaggta attttttggg gtctttttcc tgcatcacag
     4921 ccgggtctaa gccttcggct ttaaaagcat cagtgaagac tctgatattg gtttccaaac
     4981 ttaacatctt aaggcgcaat tcattaaggg ttaagtcttt tgcctcttcc tcggccttct
     5041 taatctgaag ttgcttctcg agttcggtgc tgtgctcacc gtgccgaata ttttctttct
     5101 cgattgtggc cacttgatcc tgagcttttt ttactagttc tttatgttca acaccctctt
     5161 tttctaaggc tgaaatatat ttatcgtttt cttcatgcat tttactgagc tcaagaatct
     5221 tctcttcact tttttccagc aacgattttg cgttgagcaa ttgcgcccga aggagctcgt
     5281 tttccattcg cacttcgtca atattggacg attctgtttt ctgttctggc acatctaatt
     5341 gggctttttt gtttttatcg gcatcgtcgg agctcaccgc tgtagcctgc gactgcactt
     5401 ggctgtttgt cgcgaccggt tcggagcttg ccttttttaa accatcggtc tttattccct
     5461 tcatttcacc cgacagtgtt tccgacaaac gtagcaacac tccggtttgt tgatttgtgt
     5521 cgttgtaggc gtttttgagg gtgtctaaat gaagttgaaa acgattcgct gcattcactt
     5581 gatcaggccc aaacactttt cgagaacctg cattgaagtc attccatttg tcgagaacgt
     5641 cggccagagc tttgcccgca aaattatttt ttgcttcaag attttttatt gtttcagctt
     5701 gttgcacgat cagggctacc atttccgatt cattggaaaa cgcagtttta ggaaccgtgc
     5761 ctgcatcttg tttggccgct cccgccgctg gagtggcaga accttcagct tttttagttt
     5821 cgtccgactt gcccgatggc gccgcagagt tttcttttac actcacaacg gagccacttt
     5881 cactcgtagg ttggatggcc aactttaccc cgattaattt ttcgactttg cgaaccatgc
     5941 gcgaaacacc ctcggccgca aactcgtcgg cgtcttgctc gatcgccggg ttcacaagat
     6001 gtttgagtcg catcattttt ctctgaagcg ctgctttgtg tttagctggg ttctctaaca
     6061 tttttgcgag ttcttcttca gcgtcgtctg ccgcagaaat taaggttcgt cccagggttt
     6121 tctcagattt ttctgctaaa cttcgaactc gcgctagttc ttctttaata tgttcggtca
     6181 cactttcgac tttagttttc gtctcgggtt cgtctggttg cccttcgact ttttgcaatt
     6241 cttcgtcaat tcggtctgta acactcttga tgagcgttga ttgttcttca ctcaatgcac
     6301 cacttgcgtg aacctcttgg tctaactggt cgatgtcttc tttaagcgct gctacaactt
     6361 gctcgagtac gctttgcttt ttaccggcca acttttggac ttcatctttt tggtgatctg
     6421 ttacctgctc aaccacggcc acttctccca acggaacgga ttctccggcg ggcaccactt
     6481 tcacggtttc attgaccggc tgccctgcaa catctttggc cacttcggat gctacgattt
     6541 ttgcaacgtc gatttcagtt gggatcccta gaatttttcc cccgggattg tttgcgttta
     6601 attctttgag taccgcaagc ccgtcgcccg tattgaagaa cgattctagg taagcgacgt
     6661 acgccggctt acctgccacg tgaatgtaga gcgtattgtg cttaaagttt ttcagctttt
     6721 cgagtttttc gctgggaaaa acttgcccgc tttgaatgta atgaacgatt ctatcgttct
     6781 cgcgaagcag caaataacag tcaaacggca ccaccacgtc cgcgcgaatc gactgcagta
     6841 aaatgggttc gtaatctttt tcgtcgtaag atgccatagt tacacaaaca cgcccaccct
     6901 ccttaaaatc agcataggcg cattctaaac ctcgtcggaa aattcctttc aaaaatgcag
     6961 cgagagcaag attttccgag cagtgccaac ggagcttttc gcctaaaata gactcaatga
     7021 actcgtcgca atcaaccaac gttcacgttt ccggccgagg gctcggcgca cgccgcattc
     7081 ctatttggat cactgccacg cgaatgctcg gcgcggcctt gtttttttgt gccacccttg
     7141 ccaccggact ctcaatttta cagtggggct ggattcagtt cggctacacc agtggacttg
     7201 ctttcaagtt cgcccttctt acgacgactg gcgctctagt ttttttttta gtggttcgta
     7261 acaactcact ttccagtttg tggaatctac ggcgaatcga tcgcaagtgg gtctacggcg
     7321 ggcacgccgg actcacggga atgattgcga gcattttgat aatcgccgcg ccgaccacca
     7381 cgctgttttg gagtttgagt gaaatcataa atacttttcc gacaccgacc aaacttgacg
     7441 taatcaaaga aaatccagcg ctcgactcca cgcttaaacc agtttttctg tgggagtatg
     7501 cgaccgtttt cgaccctcgg tggtttcgcg aattcgaaat cgtcttacct tacgaaaaag
     7561 gaccacccca aaaattattg ggatcgtttt atctgcaaca ccgtaaaacc cgagctctcg
     7621 gggcagcctt ctacggcaaa cgcccacggc aatatgatcg ttctacggtc gccgctcttt
     7681 tttcagagcg agctgccacc tatccgcacc ctcgacattg gccccttctt tacaatttgg
     7741 cacgggaagc cggcgcgctg ggtgccaatc gccttagctt gttcgaaacc ccgtacgcga
     7801 gtcgtatcgg tacgtctgaa agactctttt tttggagccc tgcgggtgcg gtgtttcaag
     7861 ctcgttgcga ttcgccgtgt gtgttcgaac caatgctctc gcacgtgcgt tttgccgaag
     7921 actcaaaagc gagtttaaag tctcgtcaag atctcactaa agctaaactc aaaatgcttc
     7981 ttgaaaccta ccccaaaaat gctgaacaac gtgtcctgca cgagagtgaa ctggttttga
     8041 cgttgatctc ctggctcacg ctagatcccc gcgaccccga ggcgtttttc cacattggaa
     8101 aactcgccac caaccgcgaa actgtggctt ccgccgtgcg gtatgggaaa gacctaaaga
     8161 tgtcggctcc tcaaatcgca gaactcgaaa cgcgcctggc cactttcgag tactgatagt
     8221 ctgacaacaa ataactaata tttagagcta cttacctggt aaattttgcc gttcacgccc
     8281 ctaacgcttg gtacttgttg acttaaaata catctaaata attgacttat tatgtcgata
     8341 ataaggatgt aaaacgaggt atcgggatgg gtttattgag gttcattaaa aatcgcgggg
     8401 cgggtacgtt cggacgaatc agcctactgc tgacgggggc tgcgctggcg gcaggctgcc
     8461 cgcaacaaac tgctacgacc aaagctccgg ccggatccat atcgggtgtc gtgagtagca
     8521 gtgcatcttc gagttccagt gcaacttccg ccaattcctc gagtgtcacg gcgattcaaa
     8581 ttatcacgaa ggccgattct tctggctcat tcgatagcgc aatcgtgccg gtttctggtg
     8641 gcgtctcact ggctgcatcg agactatatg acttaagcgg cgcacttatt ccttcgggta
     8701 gcataccgag ttggtttgtc gaagcgcgtc tgtttttaac ttcgactcga accagtgccg
     8761 gagctcccac caacaccaca tcgaacacgc cgtgcgccta tttcgattcg tattccgaca
     8821 acaaccctga ctcgagcggt tactacacga tcgacggtta taacgtcact caaaccggaa
     8881 tcctcgcaga tatcgaccaa tgtgcgggaa ctgccgccgc cgaactcaat caacttggtt
     8941 tttacgtacg agtggatcga cgattcatga gcagcaatga caaactgcag gtcatcgtaa
     9001 aggccaaagc gctcgaagaa ccgaacaccg ctcccacgtc cagttcgtgc gtggtgggtg
     9061 gcatattcaa cgccagcaac tgctcgaacc aagtttacac attaactcgt cgtacttctc
     9121 cgggagcgcc ggcaaaacca tttattaatt tatttccgtc tgccaaggcc tgggatcttc
     9181 ttgcggaaga cgttctgatt cccctttcgg acgacactaa tattagcacg atatccattg
     9241 atcgcgtaaa aggaggcgcg gtgttctacg gcatcaccat tatcaagatg cagtagacca
     9301 acgcgaagaa cttgaacaaa ccgtgggtgg ctcgaaaaat taaatcacga ggcgcaacat
     9361 tcacggcact ggtttgtgtc ggcagtgcat tgcttttttc aggttgccct gaacttgata
     9421 aagccaccaa cctcaacact aataaaactg cggttgcgtc gagtaccgcc tcgggcagta
     9481 gtggctcggt gccaattcat tattcagaat atcaaaacgc aaagtccgta aaagtggtgt
     9541 tcaataaaca gagtttttat aacaattttc taactaatag ttcggggtgt gccggcgacg
     9601 ctcgtttgta ctacgatccg gtcactgatc ggcaggttcc gactaaacca tcgtggctgc
     9661 gtaacgtcgc agtagaactc accaactcca acgcactcga cgggcccagt tacgccaccg
     9721 gttgcagttt ggttggaagt ggttctccca ctgctgccaa ctgcgccacc ttcgatgact
     9781 tgcagcctct cagtggcgcc gcatcgcgcg caatagtcgt gggcggcatg ggttgcgctt
     9841 ccaatacgtc gggctgctcg gcgctggcta attccccgac gctcaaaggc gatatttggc
     9901 aagcctacat tccaaacgat gcagacgaca tgacctgggc caaacaaaat cccgctttaa
     9961 gtgttttcgg cgtagcggct agtcctggac ttgcctgggc cggcggtgat ttcgacgaac
    10021 tccacaatga gttttatata ttcggcggcg tcaacgcccc cgacaacacg agcaccaatc
    10081 tagagttttc gaattcggtg gtgaaactct cgtttaacac tgacaaaagc gtgtcgaaca
    10141 ctacaccacc ggccacggta accacttcga cgcgccgtaa ctttgggggt tgggcgctcg
    10201 acggtgcatt caacgtagca ctggcagcct atttcacacc accttcgctc gtgggcaata
    10261 cgttcaccta cagttcgcgc cgcgatccta cttcgaaatc ctggtgtctc gatggcgacg
    10321 ctcagtgcag cgctactgct gccactgaat cacccgttca ggtcaacaac gaacatagtg
    10381 actattttct tttaactgga ggaatcttcg acgacgacgg agtgaccgct ccgttcgacg
    10441 acgactattt cacttcgaaa catttctgga tgtacaagcc acatggcatt tccaattccg
    10501 ctggtgccac tgcaccgagt ggtggcgatt ggattctgct ttccaacgac gaagaagctt
    10561 ccgctgctct tggtaatatt atgaaggtta tcgaaatttc aaacaccgga gtggccgaag
    10621 atttttggtc agcgtcgaat gcaacctgga acggccgagc ctttcaccgc accgtttatg
    10681 atccgaccat gaaccgcttt tatattttcg ggggtatcga ggcgagtgca aacgcaacga
    10741 ctactattcc aacgttgggg gataacgaca tttggatcta cgatccacca gcgctcgggc
    10801 gtcgccctac ggctggatgt tacgtggccg aaactccaaa taacgttgcg attcccacca
    10861 cgagcgcaac gctaggcatc ggtacttcga gcattaatgt aaatcgcgcc taccttggcg
    10921 agaaatgggt gtttcctcca ggcggctgtc tgcagcgtat ccaacacaca acagcgcccg
    10981 acgcgcggtt tgagcaagcc atggcgtttg atcgcgaaca aaaggctgtg gtcgtctttg
    11041 gtggttgcga aagcccatcc gtcattggtg ccgagggcag cagcaccgcg ggcgatccga
    11101 cgtcgaactg cgtaagctcc acagcccttt ttaacgacac gtggttgtac ttgcctccga
    11161 caacaatgga gttcgtctca aacagttatc aggatgcggg cacgagcccg tactcggtcg
    11221 acggcacgag tgctccgtat tatcggcttt ctaatatttt cggaaccgac ttttggctcg
    11281 actattttcc aatgtaccaa gacaacaaca actctagcgt agaactcacc aaaccgaccg
    11341 atgatcaaat tcttggtacg tggattcaaa tgaatccaac aaccagtccc actaaacgct
    11401 cgagcgcctc gttttattac gatcgcgccc ataaaaaatt ctacctacac ggaggtttcg
    11461 gctgtcgtaa cacgagctgc tccgacgtcg gcacgatcaa cgacctctgg gaatacacgc
    11521 ctcctaattt actaacccag tgcgatcgcg aaagtggaac gtgtagttcg aacggcacct
    11581 ggactcagat taagtcccat aacgacgccg cagccgacca acccactgcc cgcaaaggcg
    11641 cggtcaccgc gcacgctgaa ccgatgttca gctttgccga cgacttttat accgttaccg
    11701 acacctcgtg cgtgagccaa ggcccagtca ttaccggcga ttcatcgtcg aacaaacaat
    11761 acgttggtgc aatttacatc gacattgacc gcgaaaaaat cgaaagtggc cgccacttag
    11821 tcatcaactt gcgttttctc ccgttcgatc aaaacaccaa acttcccgga ctattcaaca
    11881 acaacacccc aacctacacg agcgacgaca gtgaaaacgc gtcgatcaac gaccaagcgc
    11941 tcattcgcgt gcagctcatc aacaatccag taaatttctg ggaccaaatc caatcgctca
    12001 ttcaaccgcg ctttcacgag tttttaaaca atgtgcctgt gatttcgcgc gacctcacgt
    12061 acgtcgccgg tggaactggc caggtaaccg agaaacaaat tctcattccg ctcaacggtg
    12121 tcgacacttc gttcgacatg atcaagatcg aacgcattca agggtcggtg aaattctacg
    12181 agatggcgat ttatcagtac taagactcgg ctgcaaaatc agattttttt gtcagcattt
    12241 atctcgtcat aagatattga aattacgcca tttcataaaa actctctgga acgtgtcgcg
    12301 cggacacact gtaaataata aactaaataa gtaaaatatt agattgtggg ggcctacagc
    12361 aaagaagttt ggacgtttgt ggaggacttt tatgtctcga attgctgtcg ttttagccgt
    12421 ttctttttcg ttcttttacg cacacacgct agtggctaaa gaacctgcca ccaaaaaagt
    12481 agcctcgcgc aaactcgcgt cgatcaagca attgcgtcca acactcaatc aaaccgaagc
    12541 tatgctgcaa tcgccgcacg cagcgcctcg gttgctcgat aaagaaccgt tcggatccgc
    12601 caaacactcg cgtttgggct tctacggtgt ttgtcgcgac aaacaccgta gaagccca
//
LOCUS       DAOMXO010000005        11875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_2912_length_11875_cov_7.099746, whole genome shotgun sequence.
ACCESSION   DAOMXO010000005 DAOMXO010000000
VERSION     DAOMXO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 11875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11875
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2912_length_11875_cov_7.099746"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..>6457)
                     /locus_tag="PLH57_00285"
     CDS             complement(90..>6457)
                     /locus_tag="PLH57_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRK01073.1"
                     /translation="PAPTPVPTPAPTPVPTPAPTPVPTPAPTPVPTASPTPAPVAVLY
                     VSPTGNDAGSCTQAAPCRQIRRALQFVGAGSEVRVADGDYLGFTASNLVGTAANPIVI
                     RGTGNGAIVVPSTDRGATYDRDNIILVDSAYVVFDRIRSRNAVRSGMRISTSRFISVR
                     NATVYDNGKWGIFTDFADDVTVENSTIYGTGEQHGIYFSNSNQRSIARNNVIYSNRQA
                     GIQINADRFFYPPSHWVEEVHGISRGALIENNLIYNNGVGGGAGINLDGVQDSIVRNN
                     ILYGNRAGGITNFQIDGAEGPRGMYIVHNTIVHDNSARSAVQFSASSGLNYVYNNYLY
                     HPTYVGLELLSDSDRMNIRSNHNAIDRVYFNNASVSLNNWQQQTNQDLNSRSAGSPTA
                     LFANSTARDYHLASGSALIDVGTNLSSTIVSRDFENNLRVRGNAADIGAYESAETAGP
                     SPTPVPSASPTPSPTPAPTIVPTPAPTPVPTPAPTPVPTPAPSATPAPTIAPTPVPTP
                     APTATPAPSATPAPTPVSSVGETIVRINLQKLVTSPNLVSFGLPIAQGVLNNAQNVRV
                     YPAGSNSQIIDVNTKALLYYFNSQGAPIGIQSVLIQFPTSMMPGNSMEIDVVLNRTTG
                     TVPGLGTVSYENAKFSSPQLVGVTTRTAQFSNGVYSMVENDTQQRQLFIGQEPQVLAT
                     YPDGYLANTGVLGELMSRNQVLAKAELQGLRFLSDNFEPFSISASYSDSYLYNAYLGG
                     GSTPPGVVDPLGPTDAEVEGWLYDRCATFLIGYGHYSNTGLFRHALRACSNYASRITT
                     ALDGSSPAGYFGMFTGKRYPDAKYSHIRGVFIYYALTGDERAMQAAQDMAAMWYNDSL
                     TTNYAAGRILPGDALWTERLLGASLEGMIYGFLMTNNQNYLNRAKDIVNSAYTHVSTQ
                     NQTELNALSGGNFPPQNCFIHSQAKHGEGGTNSNPWCSGWMIELVLDPLLLFQNITGD
                     SRVDEIFVRYGRFMREVGAIYTVHDQDDGYERPQLNTTLTNAGTTCYDPAQGFLARKY
                     MPAYGAGLSYGSGGALVRTVFSEGYDYEHCTDATALMSAAIRALVRRGEFNNPYNVGP
                     FQTEGQTFVAMLHEFANCADLTFQNATRLRRNPNNSHWSVNNGANLAPAVGNPGWFNT
                     NRIGYQQYSSNPQRKFSWWFNSSMNDFKLIGDTGINLDTVRAGFVQGTGCSIPASAVP
                     NSIPNPGATPVPGSSPLPTIIPTPSPSPAATAVPTATPVATAAPSATPVATATPTPAP
                     TAVPTPAPDLTGNLVVEPGFEDAQRAMTYFNSSDYTSGVTAVRSATTPIIGSASLVVS
                     IPDGGYGSAAWWTYPANGQIRAQAYTISARLRSNVPSSSDFKLCPYIYYSDSGVATLE
                     NTATDCPRATGAAGDKGVISGSITLDSNRAIDRIRIRFVQEGTSRTEFAIDELSAVLT
                     GAVGTPAQPSPTPQPSTSPTPSPTLPPGATPTPTPIPTATPIATPNPGGSQGMNIPLS
                     HPRLWFNAERLDRARTWIQSNPFTPPTSTDSAGGYGDTALHGLLTNNATGSCTAAINW
                     AMANIAAAYPITGGVACDPCRWNGEQIVLVYDWCHDYMTQDQRNTFISQMNTRISNWA
                     SNDWGGPNMYQNNYYWGYLRNEMEWAITSYHENITWASNALNFVFTNRLHNAFNPSTL
                     EGGSSRGGLAYEGSEYGPVVGVYPLIPFVTSMIHGRNLWNETDYWKEFVYGVIHQTTP
                     GLTSVPGVTGTGYTVFPFSDEERWNERFQAQSHWFPDFMAAIATVWPNDPVGRHARQW
                     SQMVGVQPWRHIRAVDVATTPLSFNTIPLDFYASGPRYMYGRNAWGQNSTSFLWQLGD
                     AHGDSIGHQHGDYGTFQIWRNGRFISRETVVYAGSSRDYLVGYNGSGSVDGAGPLGHN
                     TVLINGQVPGPQYSNRRANVERLESHPEYTFAAVDLVPPATQMQEWRREFVFIRDLET
                     TVILDRLETSDPNSVKTFINHCETDPVGTGNNGRTCVVGNQSLVMTTLLPAQRTYREV
                     NEGTNIRHQYRIEVDTAPGASQSYILTVLQAKDAAAPSLTPSVSDSDSSSFSSGTLTV
                     TLNSSHNIVFQKGIGSSGGTITVGGQTFNFRSNVQRMNVTSDGPQWQ"
     assembly_gap    6458..6557
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6558..7381)
                     /locus_tag="PLH57_00290"
     CDS             complement(<6558..7381)
                     /locus_tag="PLH57_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01074.1"
                     /translation="MLRLTSVFTRSSTIGAVFLLLGSAGCEFSTTKSSLESNPSGAAA
                     LIEPFFKANSGNLVPDPGFESGVSNFVPNQSGTSVTWVPSGNNRIAPIAGARSIQIVT
                     GGIGQNIYWRYSVLNHARFRRAERLEVSAHIRSDVASASDFGFCGVAKYAGTTAFVEQ
                     CQYVPASFGDKGKVTSFLQIDPTRDLDYIMIKVVQSGSAAMRLTIDSAQAVLFGILVN
                     NATPAPTASPTPAPSVTPSPAPTIAPTPVPTPAPTPVPTPAPTPVPTPAPTPVPTPA"
     gene            complement(7585..8736)
                     /gene="tgt"
                     /locus_tag="PLH57_00295"
     CDS             complement(7585..8736)
                     /gene="tgt"
                     /locus_tag="PLH57_00295"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRK01075.1"
                     /translation="MHFELQKQDSWSSARRGVVHTRRGTIQTPAFMPVGTFGAVRTLD
                     LADLQSLNPDIILGNTYHLMLRPGVEVFNHFGGIHGFIGWEKPVLTDSGGYQIFCLPH
                     HRTITEEGARFRSYHDGRFFMLSPEKSIEVQTAIGSDIMMVLDQCIESTADINATRKA
                     MEITHRWALRSLEARSDATQALFAIVQGGVDEALRYESVDFLTQHPFDGFAIGGLAVG
                     ETKREREDFTELVAARLPQNKPRYLMGVGTPIDLLEAVRRGVDMFDCVIPTMYAERGK
                     VFTSQGKKNVLPVANRLSDQPLDPECECSTCRQYSLGYLNHLSKCEEPTGWRLLALHN
                     QHYYQHLMRQIRNAIETDSFESLYRDLTRQWTAPLSPRRADINSSVNYY"
     gene            8996..11692
                     /locus_tag="PLH57_00300"
     CDS             8996..11692
                     /locus_tag="PLH57_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01076.1"
                     /translation="MFTARLFSNPILVPIMAVFLFTATGCEISGGGAGGSGGGSLGDA
                     IARATARFGATPTPVPSVSPSPQASASPSASPAPSVTPAPSASPAPTASPAPSATPAP
                     GASNNLVADSGFETGIDLMSDFDNNVSAYRSSTAPISGASSMIVSMPENAYGSAAWWT
                     YRANGAVTAVSYDIAARLRSNVQSTSNFAFCAFIYYSTGPSPVSACSEVSGALGDKGT
                     VRTHLDLDSTRAIESVRIRFVQNGNPRTEFAVDEMSAILNGMTGVVPTPVPTPAPTPV
                     PTPAPTLAPGATPTPTPTPAPTPVSAYPGFNYTLPVQRPFIPMSDFALAPCNSPEVVR
                     LKEWVDLGMRQRQYDSVVHDAVIIYSRAAECGWANSRSYIEYVVTEAEAHVRDAEDCI
                     NGVNGSVTCLRSWEQDLNADRDTNDPGEFTPILAKPKVSRDQYLEIGHEFHILALAYD
                     WGYDLMTPAQRLRWATYIDRALKNVWDCNGNNEFWIRSDGSPVNAGYQFSCWSINNPG
                     NNYHYSFLNATMVWAMASQDPYWLNFLQTRKFPPLANYYAQLPGGGSREGTGYGTAQM
                     NLFENYRNWKAGMQEDLSALSTHARDTIDYWVHATVPTLDRYASIGDQSRDALGYVYD
                     YHEHLVREAVKLATGTPHAQRGVWWLNNNSVARMAQRANAKYDLLQTNDQPVAPVETA
                     YYASGVGAFFARSSWNRDATWISFVAGPYEESHAHQDQGSFSIFRGSWLAVNSNMWSA
                     SGINGSVDYHNTLRFESGGSVIGQRETTAARSSMSYRQAAGITYVDANLRNAHYGDNR
                     IQAWTRSLEFEGGQLRIRDQCTVTAGVSGIWQVHVPNQPVIEGDGTIRAAGANNGVRI
                     IPVVPSSAGNVTIVPVAGRAPSWRVEIRNPAGCEFQVTLDTN"
BASE COUNT         2847 a         2842 c         3163 g         2923 t
ORIGIN      
        1 tatgttttgc cggttttaaa ttaattcagg taggcgtcct gacggtcgcc tacctgaacg
       61 tttttagcaa aaagaactac actataatgt tactgccact gagggccgtc actggtgaca
      121 ttcattcgtt gcacgtttga gcggaagttg aatgtttgac caccgactgt gatggtgcct
      181 cccgaagaac cgattccttt ttggaacaca atgttgtggg agctgttcaa cgtcaccgtc
      241 agtgtgccgc tcgagaagct cgaactatcg gaatccgaaa cactcggcgt gagcgacgga
      301 gctgctgcgt ctttggcttg taacaccgtt aagatgtagc tctgagatgc acccggagcg
      361 gtgtctactt cgatgcggta ttggtgcctt atgtttgttc cttcgtttac ttcacgataa
      421 gtgcgctgtg ccggcaacag agtggtcatg acgagtgatt gattgccgac aacacaagtt
      481 cgaccgttgt taccagtgcc aactgggtcg gtttcacagt gattgatgaa tgttttaact
      541 gagttcgggt ccgacgtttc aagacggtct aagatgacgg tggtttcgag atctcgtatg
      601 aacacgaact cacgtctcca ttcctgcatt tgagttgcgg gaggaactag atccactgct
      661 gcaaaagtgt actctgggtg cgattcaagg cgctcaacat ttgcacgacg gttggaatac
      721 tgtggacctg gaacctgtcc atttataaga accgtgttgt gtccgagagg accagccccg
      781 tccacgctgc cggagccgtt gtatccaact aaataatcgc gcgaggagcc cgcgtacacc
      841 acagtttcac gtgagataaa gcggccgttt ctccagattt gaaacgtgcc gtagtcgccg
      901 tgttggtgac cgatcgagtc gccatgcgcg tcacccaact gccacaagaa agatgtagag
      961 ttttgacccc aagcatttcg accatacatg tagcgtggcc ccgatgcgta gaaatctagt
     1021 ggaatagtgt tgaaggacag cggtgttgta gcaacgtcga ccgcgcgaat gtgtctccac
     1081 ggttgtacgc ccaccatttg cgaccactgg cgagcgtggc gacctacagg atcgttcggc
     1141 caaactgtgg cgatcgccgc catgaaatcg gggaaccaat gcgattgtgc ttggaagcgt
     1201 tcgttccaac gttcttcgtc gctgaatggg aacacagtat agcctgtgcc tgttacgcca
     1261 ggcactgaag ttaggccggg agtggtttgg tgaatgacac cgtagacgaa ttctttccag
     1321 tagtcagttt cgttccatag gtttcggcca tgaatcatcg aagtcacaaa cggaataaga
     1381 gggtacacgc ccactaccgg tccatattct gagccttcgt aagcaaggcc gccacggcta
     1441 ctaccacctt cgagtgtgct cgggttgaac gcgttgtgca ggcggttggt gaatacgaag
     1501 ttgagcgcgt tcgaggccca cgtgatgttt tcgtggtaag aagtgatcgc ccattccatt
     1561 tcattacgca aatagcccca gtaatagtta ttttgataca tgttcggacc accccagtcg
     1621 ttgctcgccc agttgctgat gcgggtgttc atttgagaaa tgaaagtgtt gcgttggtct
     1681 tgagtcatgt agtcatgaca ccagtcgtac actaagacga tttgttcgcc gttccaacga
     1741 caagggtcac acgctacgcc accggtgatc gggtaggccg cagcaatgtt agccattgcc
     1801 cagttgatgg cagcggtaca agacccagtg gcgttgttgg ttaacaaacc atggagtgca
     1861 gtgtcaccat aaccaccggc cgagtcagtg ctggtgggag gcgtgaacgg attcgactga
     1921 atccaagtac gagcgcgatc caagcgttcg gcgttgaacc acaatctagg atgtgaaagt
     1981 ggaatgttca tgccttgaga accaccagga tttggcgtgg caatcggagt tgccgtagga
     2041 atcggagttg gcgttggagt agctcctgga ggcaaggttg gcgacggtgt tggagacgtg
     2101 ctcggttgtg gagtcgggct cggctgcgca ggcgtaccca ccgctccagt gagcaccgca
     2161 cttagttcgt cgatagcgaa ttctgtacga ctcgtacctt cttgtacgaa acgtattcga
     2221 atgcgatcga ttgcacggtt tgagtctaac gttatgcttc cagatataac gcccttgtcg
     2281 ccagctgcgc cagttgctcg agggcaatcc gtcgcagtgt tttcaagcgt agctactccg
     2341 ctgtcagagt aataaatgta cggacaaagc ttaaagtccg atgagctagg tacatttgaa
     2401 cgcagacgcg ccgatatcgt gtaagcttgc gcgcgtattt gtccgttggc aggatacgtc
     2461 caccaagcag ccgaaccgta cccaccgtct ggaatagata cgacaaggct cgcacttcca
     2521 ataatcggag ttgtggctga acgaactgcg gttacacccg aagtgtagtc ggacgaattg
     2581 aaataagtca tggcccgctg tgcgtcttcg aaacccggtt caacaacaag gtttcctgta
     2641 aggtctgggg ccggcgtcgg aactgccgta ggtgcaggcg taggtgtagc agtcgctacc
     2701 ggagtagcag acggtgctgc agttgcaacg ggcgtcgcag tgggaacggc agtcgccgca
     2761 ggcgagggcg aaggagtagg aattattgta ggtaaaggcg aacttcctgg aactggtgtt
     2821 gctcctgggt ttggaattga atttggcact gcagaagccg gtattgagca gccggtacct
     2881 tgaacaaatc cagccctaac tgtatctaaa tttattccag tatccccgat aagtttgaag
     2941 tcgttcatcg aagaattaaa ccaccagctg aacttacgtt gaggattgga gctgtactgt
     3001 tgataaccaa ttcgattggt gttgaaccag cctggattac ccacggccgg cgccagattt
     3061 gcgccgttgt tcacagacca atgcgaattg tttggattgc gtcttaagcg cgttgcattt
     3121 tggaaagtga gatctgcgca gttggcaaac tcatgcaaca tcgcaacgaa cgtttgtcct
     3181 tcagtctgga atggtccgac gttgtaagga ttgttgaatt cacctcgacg gactagagct
     3241 cggattgcag cggacatgag tgccgttgca tccgtacaat gttcgtaatc gtagccttcc
     3301 gagaaaacgg tgcgaaccag cgcaccacca cttccatagg aaagacctgc gccgtaggcc
     3361 ggcatgtact tacgggcgag gaatccctgt gctgggtcgt aacaagtcgt gcctgcgttg
     3421 gttagagtag tatttaactg cggcctttcg taaccgtcgt cttggtcgtg gacagtgtat
     3481 atagcgccca cttcacgcat gaatcttcca taccgtacga agatctcgtc aacacgacta
     3541 tctcccgtaa tattttggaa cagaagaagt ggatccagta ccagctcgat catccagcca
     3601 gagcaccacg ggttactatt ggtgccacct tctccatgtt tcgcttgaga gtggataaaa
     3661 cagttttgtg gcggaaaatt accgccagaa agcgcattca actcagtttg attttgtgtt
     3721 gaaacgtgag tgtacgctga gttcactata tctttggctc gattgaggta gttttgattg
     3781 ttggtcatta ggaacccgta aatcatacct tcaagcgacg cacccaagag gcgttcagtc
     3841 caaagagcgt ctcctggaag aatgcgacct gcagcgtaat tggtggtaag actgtcgttg
     3901 taccacatcg ctgccatgtc ttgagctgcc tgcattgcgc gttcatcgcc agttaatgcg
     3961 tagtaaataa atactccgcg aatatgcgaa tatttcgcgt cgggatatct tttaccggtg
     4021 aacattccaa agtaaccggc gggactagag ccatctaaag cggtagtgat tctggaagca
     4081 tagtttgaac acgcgcgtaa cgcatgacgg aaaagtccag tattgctgta gtgaccgtag
     4141 ccgataagga aagtggcgca acgatcatag agccaacctt cgacttctgc atcggttggg
     4201 cctaatgggt ccacaacacc aggcggcgta gatccaccac caaggtacgc attatataga
     4261 taactgtcac tgtagcttgc tgaaatcgag aaaggttcga aattatctga aaggaaacgc
     4321 aagccttgga gttctgcttt tgcgagaacc tggttgcggg acattaattc accgaggact
     4381 ccagtgttgg ccaaatatcc atctggataa gtcgcaagta cttgcggttc ttgaccaata
     4441 aaaagttgtc tttgttgagt atcgttttca accatgctgt aaacaccatt ggaaaattgc
     4501 gcagttcgag tagtgactcc taccaattgt ggagaactga attttgcatt ttcgtaggaa
     4561 accgttccaa gaccagggac agtacccgta gtgcgattta gcaccacatc tatttccatg
     4621 ctgttgcctg gcatcataga agtcggaaat tggatcaata ccgactgaat tccgatcgga
     4681 gcgccttggc tgttaaaata gtagagtaac gctttagtat tcacgtcgat tatttgagag
     4741 ttgctaccgg ccggatacac ccgcacattt tgcgcgttgt tgagaactcc ctgcgcgata
     4801 ggtaacccaa acgagactaa gttcggacta gtaaccagct tctgcaagtt gatacgaact
     4861 atggtttcac caacgctcga aacaggagtt ggcgcaggcg tcgcacttgg agccggtgtg
     4921 gccgttggtg caggcgttgg aacaggagtt ggtgcgatcg ttggcgccgg cgtagccgaa
     4981 ggagcaggcg ttggtaccgg cgtcggagct ggagttggaa caggcgtagg cgcaggtgtt
     5041 ggtacgatcg taggtgcagg agtcggactt ggagttggcg atgcactcgg aacaggcgtt
     5101 ggcgaaggcc cagcggtttc agcactttcg taagcgccaa tgtcggccgc gtttccgcgc
     5161 acgcgtaaat tattctcgaa gtcccgcgag acgatcgtcg aacttaaatt cgtgccgacg
     5221 tcaatcagtg cgctaccgga agctaggtga tagtcacggg ccgtagagtt cgcaaataag
     5281 gcagtagggc ttccggccga acgtgaattc aaatcttggt ttgtttgttg ctgccagttg
     5341 ttgagcgata cgctggcgtt gttaaagtaa acgcgatcaa ttgcgttgtg attactgcga
     5401 atgttcattc gatccgaatc gctcagcaac tccaacccta catatgtagg atgataaagg
     5461 tagttattat aaacataatt taaaccacta cttgctgaaa attgaacggc ggatcgagca
     5521 gagttatcgt gaacaatcgt gttgtgcaca atatacatgc cacgtgggcc ttcggctcca
     5581 tcgatttgaa agttggttat tccgccagca cggttgccat ataatatgtt gttgcgcacg
     5641 atcgaatctt gcacgccgtc caaattaata cccgcgccgc cgccaacacc gttgttgtag
     5701 attagattgt tttcaataag agccccgcgg gaaattccgt gcacttcttc cacccagtga
     5761 cttggtggat agaaaaatcg atcggcattg atttgtatgc cggcctgccg attgctataa
     5821 attacgttgt tgcgagcaat tgaacgttgg ttggaatttg aaaaataaat accgtgttgt
     5881 tcgccagttc cataaatggt gctgttttca acagtcacgt cgtcggcgaa gtcagtgaat
     5941 atgccccatt ttccgttatc gtaaacagtc gcgttgcgta cgcttataaa gcgactagtg
     6001 gaaatgcgca taccagagcg aacggcattt cgagaacgaa tgcgatcgaa aaccacgtaa
     6061 gccgaatcaa ctaatataat gttgtctcgg tcgtaggtag cgcctcggtc ggtcgaagga
     6121 accacaattg caccgttgcc ggtgccgcga atgacgattg gatttgcagc agtaccgact
     6181 aagttacttg cagtaaatcc gaggtaatcc ccatcggcta cgcgaacttc cgaaccagca
     6241 ccaacaaatt ggagcgcgcg gcgaatctgg cgacaaggag ccgcctgtgt acaactgccg
     6301 gcgtcattac cggtcggtga aacgtaaagc actgcaaccg gtgcaggagt tgggctcgca
     6361 gttggaaccg gagttggtgc cggagtagga actggagttg gtgccggagt aggaactgga
     6421 gttggcgccg gagtaggaac tggagttggc gccggagnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnngcc ggagtaggaa ctggagttgg cgccggagta ggaactggag
     6601 ttggcgccgg agtaggaacc ggagttggcg ccggagtagg aactggagtt ggagcgatcg
     6661 ttggcgctgg gcttggtgtt acgctcggtg ccggcgtcgg cgaagccgtt ggtgcaggcg
     6721 tagcgttgtt aactaatatt ccgaataata cggcttgagc gctatcaatc gttaagcgca
     6781 tggcagcgga accgctttga accactttga tcattatgta atcgaggtcg cgagttgggt
     6841 cgatttgaag aaaagaagta acctttcctt tgtcgccaaa ggaagcggga acgtattggc
     6901 attgttctac aaacgcagta gtgccggcgt atttggccac accacaaaaa ccgaaatcag
     6961 aagcactggc aacgtccgaa cgtatgtgag ctgaaacttc aagtcgttct gcgcgacgaa
     7021 agcgcgcgtg atttaatacc gaatagcgcc aataaatatt ttgaccaatt ccacccgtga
     7081 cgatttgaat actacgagca ccagcaatgg gtgcgatgcg attatttccc gaaggcaccc
     7141 aggtcacgct tgtgccgctt tggttcggaa caaagtttga cactccggat tcaaatccgg
     7201 ggtcgggtac taagtttccg ctgttcgctt taaaaaatgg ttcgattagg gcggctgcgc
     7261 ccgaggggtt cgattctaat gaactcttgg tggtgctaaa ttcgcagccc gctgatccaa
     7321 gcagcaaaaa tacggcgccg atggtcgagg aacgcgtaaa aacgctcgtt aaacgtagca
     7381 tggcgagaac cccctctatc aacctggctt tgaaggcccg gttttttgtc ggctgacccc
     7441 ttactttcag cgcgaccgct gcacaccacc tacgtaccgc atgctcaaga tgttgacttc
     7501 aactatcaag tatagcacca atgcaacgcg atgcaatgag gactttactt tgatcgatct
     7561 agctgaattt attgagcaag attattaata ataattaaca gaggaattaa tgtcagctct
     7621 tcgagggctc agtggggcgg tccactggcg ggttaaatcg cgatacaggc tttcgaaaga
     7681 atcggtctcg atagcgttcc gaatttgcct cattaaatgt tggtaataat gctgattatg
     7741 gagcgctaac aaccgccatc cggtaggttc ctcacacttc gaaaggtggt taagataacc
     7801 aagcgaatat tgacggcagg tagaacattc gcattctgga tccaacggtt gatcgctcaa
     7861 ccggttggcc accggcagaa cgtttttctt accctgcgaa gtaaacactt tgccacgctc
     7921 cgcgtacatg gtgggaatga cgcaatcaaa catgtccaca ccgcggcgaa ccgcttctaa
     7981 taaatcgatc ggagtgccca cgcccataag atatcggggt ttgttctgtg gcaatcgtgc
     8041 agcgacgagt tccgtaaaat cttcacgttc ccgtttagtt tcaccgacag caagcccgcc
     8101 aattgcgaag ccgtcgaatg ggtgttgagt caaaaaatca acgctttcat agcgaagcgc
     8161 ctcatccacc ccaccttgta cgatggcaaa tagggcttga gtggcgtcgc ttcttgcttc
     8221 gagacttcgc aaggcccagc gatgagtgat ctccatggcc tttcgagtag cattaatatc
     8281 cgctgtagat tctatgcact gatcgagcac catcatgatg tccgagccaa tcgcagtctg
     8341 aacttcaatg ctcttttcag ggcttaacat aaagaaacgt ccgtcgtgat aacttcgaaa
     8401 acgagcgcct tcttcggtga tcgtgcgatg gtgaggaagg cagaaaattt ggtaaccgcc
     8461 ggaatccgta agaacgggtt tttcccaacc gatgaatccg tgaattcctc cgaagtgatt
     8521 aaagacctca accccagggc gcaacatgag gtgataggtg ttaccgagaa taatgtcagg
     8581 atttaacgac tgaagatctg ccagatcgag agtgcgaact gccccaaatg taccaaccgg
     8641 catgaaagcg ggcgtctgaa tggtgcccct acgtgtgtgt acgacccctc tgcgggcgct
     8701 cgaccaagag tcttgttttt gcagctcaaa atgcattcgg aaacgacctc tggcctcggg
     8761 taccagaagt ttataatgaa atccagactg aatcgatcat aatatagatt tattcaatga
     8821 tttcatgttt ttagattcgt attgttgctt tgcgtcgtat ccatgctaga atagataaca
     8881 gatcttaaca atttcgttgt gcggtacgtg tgcagagtag acgggacgct gagaaaatgg
     8941 ggtcagcgcc cagaattgga ccgagggtcc gattcgattg agggggctct gtcgtatgtt
     9001 tactgcgcgt ttgttttcaa atccaatact agttccgata atggcagtct ttctgtttac
     9061 cgccacagga tgtgaaattt ccggtggcgg agctggcggc agtggcggcg gttctcttgg
     9121 tgatgcgatc gcccgagcta ccgctcggtt cggggcaact ccaactccgg tgccttcggt
     9181 ttcaccgtca cctcaagcat ccgcgtctcc ttctgcctct cccgctccat cggtgacccc
     9241 agcaccgtca gcttctcctg cacccactgc ttctcctgcg ccttctgcta ctccggctcc
     9301 gggagcatcg aataatttgg tcgcagattc tggttttgaa actggaatcg atctcatgag
     9361 tgactttgac aacaatgtta gtgcctaccg ttcgtcaacc gctcccattt cgggggcctc
     9421 cagcatgata gtgtcaatgc cagaaaacgc ctacggtagt gctgcatggt ggacctatcg
     9481 cgctaacggc gcggtcactg ccgtgtccta cgatattgca gcccgcctgc gatcaaacgt
     9541 gcagagtact tcgaactttg ctttttgcgc cttcatctat tattcgaccg ggccttcgcc
     9601 tgtttcggct tgtagcgaag taagtggcgc actgggcgat aaagggaccg tacgtactca
     9661 cttagacttg gattccactc gtgcaattga gtcagtgcgt attcgttttg ttcaaaacgg
     9721 aaatccacgc accgaatttg cagtagacga aatgtcagcc atcttgaacg gaatgaccgg
     9781 agtagtacca acgccagtgc caactcccgc tccaacaccg gtgccgactc ccgctccgac
     9841 tctcgcgccg ggcgctactc caacgccaac gcctactccg gcgccaactc cggttagcgc
     9901 ttatcccgga tttaactaca ctctaccagt tcaacgtccg ttcattccta tgagcgattt
     9961 cgctttggca ccgtgtaatt cgccagaagt ggttcgtctt aaagagtggg tcgacctggg
    10021 tatgcggcaa cggcaatatg attctgtcgt tcatgatgca gttattattt attccagggc
    10081 ggcagaatgc ggctgggcta attcgcgaag ttatatcgaa tatgtagtaa ccgaagctga
    10141 agcgcacgta agagatgcag aagattgtat taacggtgtt aatggttcag taacttgtct
    10201 gcgctcttgg gaacaagatc tcaacgccga tcgtgatacc aatgatcccg gtgagttcac
    10261 gccaattctc gctaaaccca aagtgagtcg ggaccagtat ttagaaatcg gacatgagtt
    10321 ccacattcta gcactagctt acgactgggg ttacgattta atgactcctg cacaacgctt
    10381 gcgctgggca acctacattg accgagcgtt aaaaaacgtt tgggattgta atggaaacaa
    10441 cgaattttgg attcgcagcg acggaagtcc cgtaaacgct ggttatcagt ttagttgttg
    10501 gtcgatcaat aatccgggta acaactacca ttacagtttt ttaaacgcga ctatggtttg
    10561 ggcgatggca agccaagatc cttactggct gaactttctg caaacccgaa aatttccgcc
    10621 actggctaac tactacgctc agttgccagg tgggggtagt cgtgagggta ctggttacgg
    10681 caccgctcag atgaatctct tcgaaaatta ccgaaattgg aaagcgggca tgcaggaaga
    10741 tctttcggcg ctttccactc acgcgcgcga cactatcgac tactgggtgc acgctacggt
    10801 gccgactctc gatagatatg cttcaattgg tgaccaatct cgcgatgctt tggggtacgt
    10861 gtatgactat cacgaacatt tagtgcgtga agccgtcaag ctcgcaacag gtactccaca
    10921 tgcccaacgc ggcgtgtggt ggctcaacaa caacagcgtg gctcgaatgg ctcagcgggc
    10981 aaatgcgaaa tacgacctat tgcaaaccaa cgatcaacct gtagctccgg ttgaaacagc
    11041 gtattacgca agcggagtgg gtgccttttt tgcccgaagc agctggaacc gcgatgccac
    11101 ttggatcagc tttgtggcag ggccatacga agaatcccat gcgcaccaag accaaggctc
    11161 attcagtatt tttcgcggca gttggctggc ggtgaattct aatatgtggt ctgccagcgg
    11221 gatcaatggt tcggtagact atcataatac gttgcgtttc gagagcggcg gatcagtgat
    11281 aggtcagcgt gaaaccacag cggcgcggtc ttcgatgtct taccgtcagg ctgcgggaat
    11341 tacttacgta gatgccaatt tacgaaacgc ccattacggc gacaatcgca tacaagcgtg
    11401 gactcgcagt ctggaatttg aaggcggtca actacgaata cgtgaccaat gtacggtgac
    11461 tgcaggggtt tcgggaatat ggcaagtcca cgtgccgaac caaccggtga tcgaaggcga
    11521 tggcacgatt cgagccgcag gcgcgaataa cggcgtgcgc attattccag tagtaccgag
    11581 cagtgccggc aacgtgacga tcgtgcctgt agcgggccgc gccccaagct ggcgcgttga
    11641 gattcgcaac cccgccgggt gcgaatttca ggtcacttta gatacgaact aaaacgtttt
    11701 ggtcgggaaa ttgcgcttaa tttcgcatgg tgttatgccg ctcctttagc gtttggagtg
    11761 gaaaatgaaa aaacatctta attcgtgccg gcatttcgcc ggtgtggttc tgttattttt
    11821 gatcgcattt actgcgcggg ctgcggaatt tccggatacc tgccaattcg acaag
//
LOCUS       DAOMXO010000006        11837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_2925_length_11837_cov_4.700136, whole genome shotgun sequence.
ACCESSION   DAOMXO010000006 DAOMXO010000000
VERSION     DAOMXO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2925_length_11837_cov_4.700136"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2239)
                     /locus_tag="PLH57_00305"
     CDS             complement(<1..2239)
                     /locus_tag="PLH57_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01077.1"
                     /translation="MQVSSNFSDHSLQTLNWGLITSSLSERAMSDLGKARCESHRPFT
                     SPQAAERFQTKTAEALRLANNGSTVHSFVSGTQDPSEQLERASREAILMPLELWNVGH
                     FMQSVGFLGSELRSAKTLAPVLAFEFETAPSVPAVCNEISRAVSSEGLILDSASSELL
                     RLRRLRSDRRSAFETQLESKVKEWHAKGLLQDKFYDVMEGRFVVPVKVEQQSKLEGAL
                     VGRSNTGQSVFMEPLELTTSNNELKEIELSIRSEELRILRELSAKIGALAPVFQLWVE
                     PVAEFDLVFASALFARDFKLTRPKFSEDRKLILRGLFHPQLSSQGIEAVKNSFALETE
                     APPQAGRRCILISGPNTGGKTVLIKAAALATAMAQAGLYIPADDACVLPFYKNLYALI
                     GDEQSIAAGLSSFSAQIAELKRALNEAAIPSLIVVDEILSSTDPEEASALAQAFIEEC
                     LARGHHLLVTSHFSELSARVRENKHAAVGAMEFRNGRPTYELRLDELGSSHAIEVAAR
                     LGFPPKLLERARALVSQGKVDYQNALQELRAKEAALELAHRQETDRLRSENRDLKNRL
                     EQFLKDSEEKIRIAIDQALRAIAVERARKTSSVSVSAKLKAQTDAIAQEATRKVGSAA
                     QTAFGEALEIDAPTKDRKTVEFKVGTKIRLRDKRYVHGVILELHPDKGTAVVQLGILR
                     MEKALTELDLFEEAGKKHRSSGIHVETPISTEVFSKKLDLRGRRTEEALAAAESFLDN
                     ALRAGM"
     gene            2258..3772
                     /locus_tag="PLH57_00310"
     CDS             2258..3772
                     /locus_tag="PLH57_00310"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HRK01078.1"
                     /translation="MSFNYLEIERALSEIHRSANEGAEDFLVRSIEFVEFPLFKKTEL
                     RTSSAVVLEDVCLAFRLADYSLVFSLKHPWVGLFWMRHPAFRKIKQTSMAGFSLSQFD
                     SLKGQRIDLLEQVSGDRVVKLHFSSGAQLEVELFGMRPNWKLTGPINSQWRPDSAQQN
                     STQRALRDAGPKIQKNFEIRDFGALHNTTIWLEGAYYEYRALRRTAYLRALRGWAAGE
                     LEKRLTHLLKVRGAMQEQLRETREGDRLRKAGEELKAVLYQHPPAFKTSHLPGFAYEL
                     DAEKTLAENADLFFDRSKKLVRTSKEVTKRLQEIESRVERQRKLIQRLRDQSLSTTNS
                     QLPQNFDLEVTKISEFLVEAGLDNQVDKKLDGREFKQKEKMIAAGARQFRSRDGQMIW
                     VGRNQDENEKIVIRVANGNDIWMHLKGRPGAHVIVQIARGKSASLETLLDAAALVCHY
                     SGIGEQEKAEVDYTFRKYVKRLSGAKFAVTYSQNKTLVVKQDADRLKRLLAQDY"
     gene            3802..4395
                     /locus_tag="PLH57_00315"
     CDS             3802..4395
                     /locus_tag="PLH57_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01079.1"
                     /translation="MDSSLVWFFSAIAIGALHAFEGDHLAAVSAFVARRPHWRNAMRF
                     GGLWALGHAASLLVFAAVLVGLKTTFLPTTEAWFERVVGLVLVGLGTSLLFRAVRVRD
                     SYVHTHSGRAPLLMGLLHGLAGTAGFLTQAVAFQQKGLGAVTCVALGFSIGVLVAMAG
                     YSLVLGRVFSLGTSPGVLRAIQGLSGALIVVVGITRM"
     gene            4440..4808
                     /locus_tag="PLH57_00320"
     CDS             4440..4808
                     /locus_tag="PLH57_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRK01080.1"
                     /translation="MDQNFDDVNQDNLELSPNEALEKFRGNSAVFVDVREAPEWESGC
                     VPSSFRLPLSLLASGRAAESSVFATLDALDAPIIVYCHGGVRSLTGAVVLRKMLRRDD
                     VQSMSGGIIAWSGELEVPTT"
     gene            complement(4801..6348)
                     /locus_tag="PLH57_00325"
     CDS             complement(4801..6348)
                     /locus_tag="PLH57_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRK01081.1"
                     /translation="MARRIGLWFMFSLLLTTLSAHARNDNVLRVRTFGEPATLDWNRA
                     FTPVEATLVRNIQEGLVAVGTNLKVVPGVAERWTLSADGKTYKFFIRKNVKWSDGKPL
                     LAQHFVDSWERLLDPETKATTAYLLFDIEGAEDYHHGKGPITGVAMRALNSHTLEVRL
                     RNPVSYWLWIPTFWSTFPIRKDLIAEHGTAWDKPGKLISIGPYVLSSHEPNKRITLDR
                     NPHYWGKVGNIDQIHAYLISDDKLAVEKFESGALDFVTRIGSADVRRLSTTKELRKWT
                     ELRTVHLDLNPNTLPTDSLALRKAVFHAIDRKRLSRIVSGAYEPATSFVPPGLLSFQN
                     NIGLEFDLKKAKSYLKKVNPEVLSSLELVAPGFDDFILVGDFLQTELKKNLGIDLKIS
                     TFEPKRFYSSAVNFGGYHMILTHWTADYPDPDNFFSIFLSNAGNNRVNWKNTQYDELV
                     LKGRTTSDLKARKKLYADAHKLLLDQHAIAHPLYYGKNHALVRKNIRGFAPTATNSYL
                     FKDFSIK"
     gene            6435..7391
                     /locus_tag="PLH57_00330"
     CDS             6435..7391
                     /locus_tag="PLH57_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRK01082.1"
                     /translation="MRYAFLFSFLISISAFADGVGIESDSGIHGPNYGDQIQELKNLE
                     AEFAGVGQYVEYGKTVEGRPLAMLRIGGTQVDESTLGGKAVYIGGATHGDEYLNIEDR
                     LARIFLTEWAQGDRSTMPELQRFVAEGGVIYIAPILNPDGYEGRYRENRNGVDLNRDF
                     GLPPENLPGFTQPETRSLVTTLQRDAAAHRLNLRVAVDYHCCIGALLYPWSYDEGPEM
                     PREHEAAYQALGNAMLRPFKDVGRYRLGKTPTLLGYNAVGTSKDYYYAVHGTLAFTFE
                     GVFRKEQANLAQHIAMWESVFRFLNAEKAAEAEWEWTYGATY"
     gene            7375..8754
                     /locus_tag="PLH57_00335"
     CDS             7375..8754
                     /locus_tag="PLH57_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRK01083.1"
                     /translation="MELHTSRTNGPNPGAAGPKGHWFFGNLLDRRRRPLELYADSTRV
                     YGKTVRFRMGPKTVWLLSNPVDVQLVLHDQAKKFVKGYGYDILEIFLGRGLLTSDGSF
                     WHRQRRLIQPVFHRSNLVGFAQVMQDEADALVLRLRGQVEVDVTTTMLKLTMEIVGKT
                     LFGTNVSDHTSEVSPIISELIEDANRRLLSVVPLPVSVPLPRNLRTRRLIERLDGLMS
                     KMIEERRNQKENDPKDKGIDLLSLLIAATDDQTNERMTDRQLRDEAMTIFVAGHETTA
                     NALAWTLILLAQHPEVQTRAREEVQKPNAAPGLFERVLSYEYLGRVIDESMRMYPPAW
                     VVSRQSTEPVALSQTVVHPDEIVAISSWVLHRDPDFWKDPQTFDPERFTADECAKRHK
                     YSYIPFGAGPRQCIGTQFARVELLTVLSTLLRNYRFELSGGSQQKIEPHPLVTLRPRG
                     RVPMKFIPI"
     gene            complement(8759..9013)
                     /locus_tag="PLH57_00340"
     CDS             complement(8759..9013)
                     /locus_tag="PLH57_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HRK01084.1"
                     /translation="MTAKESALNKKVKVSPELAKFVGADEISRADVTKKLWDYIKKNN
                     LQDPNDKRTIVPDATLAPLIGGEPINMMKMTSVLNKHFIK"
     gene            complement(9079..>11837)
                     /locus_tag="PLH57_00345"
     CDS             complement(9079..>11837)
                     /locus_tag="PLH57_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRK01085.1"
                     /translation="VSVSGAMTDLELRKVAKHLENEIEAIPGVARVVHRGLRDLEIRV
                     EADQNKLARYRLSLDDLIRSLKGQNVSIPGGTVEADPEHPERDERIVRTIGDFLTLED
                     VRKTVIRANDLAQGVRVGDVAKVYDDLERATTINGTDGLPAFGLTVLKKEKADAITLV
                     DSLKTKLNQIGKNLDPRVKLSFLNDMSEFIRRRLNILTGNLMIGLVLVLFLLSLILPI
                     RVALIVSLGIPFAFLGTMLIFQWMGFSINLISMLGLIIVSGMLVDDAIVVTDNSVRLI
                     EEGMEPRAAAIEGASQMVAPVTASVLTTIVAFLPMMFMSGIFGKFVREIPLGVICALL
                     ISLFEGFFILPGHIARFVRVPSVAANGGERKSRSRVRQKLDSTLLKTRGFWESWVVPR
                     YIKLLTVTLRHRWVMMLALVFVFSSSIGLAVKGMRFILFPPEGIEIFFIRAQAKVGSS
                     LETTARLTKQLEKIVEQLPKNEVKNYITSIGIQQQDPNDPNTKRGSEYAQITVFLTPE
                     TERKRKAIEIIEAARQIGGTPKDFVRVSFERVSPGPPTGKPVSIGVRGKTYEDIMPAV
                     KELKQLLTGVTGVTDINDSYVLGKPELRVAVNPTEAAAAGLTVAGIGTTVRAAFEGIV
                     ATSVRNLDEEIDVRVSLLEKQRSEENVLREILVPNSFGNLVPLASVARISEAQSLAVY
                     EHEANQRQVKVTAEVDVTKSSALEANNAVRAMLPDLNQKFPNVTIAFGGEDEDTQESL
                     KSLMRAFGIAFFAIFLILVMTFKQLLQPLLVLITLPLGIISVIWTLFFHGLPLSFMSM
                     LGIIALGGVIVNNAIVFVDFVNQRRINGMDRFESIIQTAKLRIRPIFLTTTTTVAGLL
                     PTAYGIGGLDRFVVPIALALGWGLAFGSLLTAFVFPSAIAVLDDISEFLRRKFPGLYR
                     SYDR"
BASE COUNT         2903 a         2888 c         3045 g         3001 t
ORIGIN      
        1 ccatgcccgc acgcagagcg ttgtctaaaa acgactcagc agcggccaac gcttcttcgg
       61 tgcggcggcc acgcaaatcg agtttttttg aaaaaacctc ggtcgaaatc ggagtttcca
      121 catgaatgcc actcgagcgg tgtttcttac ccgcttcttc gaacaagtct aactctgtca
      181 gtgctttttc catgcgcagg attcccagtt gcaccacggc ggtgcctttg tctggatgta
      241 gctccaaaat cacgccgtgg acgtaccgtt tgtcgcgcag ccgaattttg gtgcccactt
      301 tgaactcgac ggtctttcgg tctttggtag gagcatcaat ttcgagggcc tcaccgaatg
      361 cagtttgcgc agccgagcct acttttcggg tcgcttcttg ggcgattgca tctgtctgag
      421 cctttagttt tgcgctaacc gaaaccgaag acgtcttacg agcgcgctct acagcaatcg
      481 ctcgcaacgc ctggtcgatc gcgatacgaa ttttttcttc ggaatctttt agaaattgtt
      541 ctagccgatt tttaaggtcg cggttttcac tgcgcaggcg gtcggtctct tgacggtggg
      601 ccaattcgag tgccgcttct tttgcgcgca attcctgaag cgcattttgg tagtccacct
      661 tgccttgcga cactagcgcc ctggcgcgtt ccaagagctt tggcggaaac cccaaacgag
      721 cggccacttc aatagcgtga ctcgacccca gttcgtcgag tcgtaactca taagtgggcc
      781 gaccattgcg gaactccatg gcgccgactg ccgcgtgttt gttttcgcgc acccgagcgg
      841 aaagttcact aaagtgactg gtgacaagca ggtgatgccc gcgagccaaa cattcttcga
      901 taaacgcctg cgcaagtgcc gatgcttctt cggggtcggt gcttgaaaga atttcgtcga
      961 ccacaattaa actaggaatc gccgcttcgt tcaacgcgcg ctttagttcg gcaatctgag
     1021 ccgaaaaact cgaaagcccg gctgcgatac tttgctcgtc gccaatgaga gcgtagaggt
     1081 ttttataaaa aggcaagacg cacgcgtcgt cggccggaat gtacagtcca gcttgcgcca
     1141 tggcggttgc gagcgctgct gctttaatga gtactgtttt gccaccggta ttcgggcccg
     1201 aaatcaagat gcagcggcgc ccggcctggg gcggggcttc ggtttccaat gcaaaactgt
     1261 ttttaaccgc ttcaatgccc tggcttgaga gttgcgggtg aaataatcca cgtaaaatga
     1321 gtttgcggtc ctcggaaaat ttaggccggg tcagtttaaa atcgcgcgca aaaagtgccg
     1381 atgcaaatac taaatcaaac tcggctaccg gctcaaccca aagttgaaaa actggagcca
     1441 atgcgccaat ttttgcgcta agttcccgca aaatccgcaa ttcttcgctt cgaatcgaaa
     1501 gttcgatttc tttgagttca ttattggatg tcgtgagctc caatggttcc atgaacaccg
     1561 attgcccagt gttgctgcgc cctaccaagg cgccttcgag tttcgattgt tgttccacct
     1621 ttacgggaac cacaaaacgg ccttccatga cgtcgtagaa cttatcttgc agcaaaccct
     1681 ttgcatgcca ttctttaact ttagattcga gttgagtttc aaaggccgaa cgacgatcgg
     1741 accgcaggcg gcgcaggcgc agtagttcgc ttgaagccga atcgagaatg agcccttccg
     1801 acgacacagc tcgcgaaatt tcgttacaga ccgctggcac gctgggggct gtttcaaact
     1861 cgaaagccaa tactggcgcc agggttttgg cactacgcaa ttctgaaccc aaaaaaccca
     1921 ccgactgcat aaaatggccg acgttccaca gttcaagtgg catgagaatg gcctcacggc
     1981 tcgcccgttc taactgttcg cttgggtctt gagtacctga gacgaacgag tgtacggtag
     2041 acccattgtt ggcaagtcgc agggcttcgg ccgttttggt ctgaaatcgt tcagcggcct
     2101 gagggctggt aaacgggcgg tgtgattcgc aacgcgcttt tcctagatcg ctcatggccc
     2161 gttccgagag cgacgacgtg ataagtcccc aattgagtgt ttgcagcgaa tggtccgaaa
     2221 aattagatga aacctgcatg gagtctttca taccagtatg agctttaatt atctagaaat
     2281 tgaacgggca ttaagcgaga ttcaccgctc ggccaatgaa ggcgcagaag actttcttgt
     2341 acgctcgatc gagttcgtcg agttcccgtt atttaagaaa accgaattgc gcactagtag
     2401 tgccgtggtg ctcgaagatg tgtgtctggc ctttcgtctt gcggactatt cgctggtgtt
     2461 ttcactcaag cacccgtggg tcggactttt ttggatgcgg catccagctt ttcgaaaaat
     2521 aaaacaaact agtatggcgg gatttagtct atcgcaattt gactctctga aaggtcaacg
     2581 catcgacctc cttgaacagg tatcgggcga tcgagtagtg aagcttcact tttccagcgg
     2641 ggcgcagctt gaagtcgaac tctttggtat gcgcccgaat tggaagttaa ccggaccaat
     2701 aaattcacag tggcgaccgg attcagcgca acaaaattca acacaacgcg cattgcgcga
     2761 cgccggtcca aaaattcaga aaaattttga aattcgcgat ttcggtgcac ttcataacac
     2821 cacgatttgg ctagaaggcg cttattacga ataccgtgcg ctacgtcgca cggcctattt
     2881 aagggcgctt cgtggctggg ctgccgggga gcttgaaaaa cgactcacgc accttttaaa
     2941 agtgcgtggc gccatgcagg agcagctgcg agaaacgcga gaaggggatc gactgcgcaa
     3001 agccggcgaa gagctcaagg cggttcttta ccaacacccg ccagctttca aaacgagtca
     3061 tcttcctggt tttgcctacg aattagatgc tgaaaaaact ttagcggaaa atgctgatct
     3121 tttttttgat cgcagcaaga agttggttcg tacttcgaag gaagtcacga aacgattaca
     3181 agaaattgag tctcgcgttg agcgtcaaag aaagttaata cagcggcttc gtgatcaaag
     3241 cttgagcacc acgaactccc agctcccgca aaattttgac ctcgaggtta cgaagatttc
     3301 agagttctta gttgaggccg gactcgataa ccaggtcgat aaaaaattag atggccgcga
     3361 gtttaagcaa aaagaaaaaa tgatcgccgc gggtgcgcgc cagtttcgca gtcgcgacgg
     3421 ccaaatgatt tgggttggtc gcaaccaaga cgaaaatgaa aaaatagtca ttcgcgtcgc
     3481 caacggcaac gacatttgga tgcacttaaa agggcgcccc ggcgcccacg tgattgtgca
     3541 aattgcccgc ggaaaaagcg cttcgctaga aacgctactc gacgcggcag ccctcgtttg
     3601 ccattattcc ggcattggag agcaagaaaa agctgaggtc gactacacct ttcgcaaata
     3661 cgtcaagcga cttagtggcg cgaaattcgc ggtcacctat tctcaaaata aaacgcttgt
     3721 ggtgaaacaa gacgccgacc gcctaaagcg gcttttggct caggactatt aagattttta
     3781 agttttattg gaagggttaa aatggacagt tcgttggttt ggtttttttc ggcgatcgca
     3841 attggcgcgc tccacgcgtt tgagggcgat cacttggcag ccgtcagcgc atttgtggcg
     3901 cgtcgcccac actggagaaa cgcaatgcgc ttcggcggac tttgggccct aggccatgcc
     3961 gcaagcctgc tggtatttgc ggcggtattg gtgggactaa aaaccacgtt tttgccgacc
     4021 accgaagcct ggtttgagcg agtcgtaggg ttagtattag tagggctcgg caccagcctg
     4081 ctttttcggg cggttcgagt tcgtgattcc tacgttcaca cgcattccgg tcgggctccg
     4141 ttattaatgg gtttgcttca tggactagcg ggtacagcag gttttttgac tcaagcggtg
     4201 gcctttcagc aaaagggcct gggagctgtc acctgcgtag ctctgggttt tagcataggc
     4261 gttctagtgg cgatggcagg ttattcctta gtattagggc gggtgttttc gcttggaact
     4321 agtccgggcg tcttgcgcgc gattcaaggg ctttccgggg ccctgattgt tgtcgtcggt
     4381 attactcgaa tgtgatagta aaccaggaag ctatattcag gagaacatcg ccaatgacta
     4441 tggatcaaaa ttttgacgac gtaaatcaag acaaccttga actttcacca aacgaagctc
     4501 tcgaaaaatt tcggggtaat tcggcagtgt ttgttgatgt acgcgaagcg ccagagtggg
     4561 aaagtggttg tgttcccagt agctttcggt tgccactttc attgcttgct agtggtcgtg
     4621 ccgccgagtc ttcggtgttt gctaccctgg acgcactcga tgcgccgatc atcgtttatt
     4681 gtcacggcgg tgtgcgatcg ctgactggag ccgtggtgct acgcaaaatg ctaaggcgcg
     4741 acgacgttca gagcatgagt ggtggaatta ttgcttggtc cggcgaactc gaagtgccaa
     4801 ctacttaatt gaaaaatctt taaacaagta agagttagta gcggtaggtg cgaaacctcg
     4861 aatgtttttg cgcaccaacg cgtgattttt gccgtaatag agtgggtgag ctattgcgtg
     4921 ttggtctaaa agtagcttgt gtgcgtcggc gtaaagtttc tttcgcgcct taagatcaga
     4981 cgtggtgcgc cccttcaaca ccaattcgtc gtactgcgtg tttttccagt tgacgcgatt
     5041 gttgccagca ttgctgagaa aaattgaaaa aaagttatcg gggtcggggt agtcggccgt
     5101 ccagtgcgtt aggatcatgt gatagccacc gaaattgacc gccgacgaat aaaaacgttt
     5161 gggttcaaac gtactgattt tcagatctat gccgagattc tttttaagtt cagtctgcag
     5221 aaaatctcca actaaaataa agtcgtcaaa accgggcgct accaattcga gcgacgatag
     5281 gacttcgggg ttcacctttt ttagatatga ctttgctttt tttagatcga attcgagtcc
     5341 gatattattt tgaaagctca ataacccggg cggtacgaaa gaagtcgctg gttcgtaggc
     5401 accagataca attctagaaa gtcgttttcg atcaattgcg tgaaaaaccg cttttcgtag
     5461 tgccaacgag tctgttggca aagtattggg attaagatct aaatgcacag ttcgtaattc
     5521 ggtccatttt cgaagttctt tagtagttga aagtcttcga acgtcggccg atccaatgcg
     5581 agtgacaaaa tctagagcac cagattcaaa tttttcgact gcgagtttat cgtccgaaat
     5641 caaataggca tggatctgat cgatatttcc aactttgccc caatagtgcg ggttgcgatc
     5701 aagagtgatg cgcttgttcg gctcgtggga agagagtacg tagggtccga tggaaattag
     5761 ttttccaggt ttatcccacg ctgtgccgtg ttcggcgatc aggtctttgc gaatgggaaa
     5821 cgtgctccaa aatgtgggga tccacaacca gtaggaaacc gggttgcgga gtcgaacctc
     5881 cagcgtgtga gagttgagtg ctctcattgc gacaccggta atggggcctt ttccgtgatg
     5941 atagtcttct gccccttcga tgtcgaatag aaggtaggcc gtggttgctt tggtttcagg
     6001 gtcgagtaat cgttcccaac tatcaacaaa gtgctgcgcc aataggggtt ttccgtcaga
     6061 ccattttacg ttttttctta taaaaaattt gtaggttttg ccatcggcac tgagagtcca
     6121 acgttcggca actccaggca ctacttttag gttggtccca acggccacca acccctcttg
     6181 aatgtttcgt actaacgtag cttctaccgg cgtaaaagct ctattccagt cgagagtcgc
     6241 aggttcacca aacgtgcgga cgcgcagaac attatcgttg cgcgcgtgcg cagataaagt
     6301 tgttaaaagt agcgagaaca tgaaccataa accgatgcgg cgtgccatga cgaactccgt
     6361 ttttttaatc attcttgcac aggatctcag tttggcaagg atgacccgga gcaagacacc
     6421 atgggaggcg tcgcatgcga tatgcttttc ttttctcttt tctaatttct atttccgctt
     6481 ttgcagacgg cgtcggtatc gaatccgatt ccggaattca cggtccaaat tatggcgatc
     6541 agatccaaga attaaaaaac ctagaggcag aattcgccgg tgtgggtcaa tacgtagaat
     6601 atggcaagac ggtagaaggg cgaccgcttg caatgttgcg tatcggcgga actcaggttg
     6661 acgaatcaac gctaggaggc aaggcggttt acatcggcgg tgcaacccac ggcgatgaat
     6721 atctcaacat tgaagaccgc ctcgcacgaa tttttttgac ggaatgggcc caaggcgacc
     6781 gatccaccat gcctgaattg cagcgcttcg ttgccgaagg cggagtcatt tacatcgcgc
     6841 ctattttgaa tcccgatggg tacgaggggc ggtatcgtga aaatcgaaat ggggttgacc
     6901 ttaatcgcga cttcggtttg ccgcccgaaa acctaccggg gttcacgcaa cctgaaacac
     6961 gcagtttagt aacgacgctt cagcgcgacg ccgccgctca tcgtctgaac ttgcgggtgg
     7021 ctgtcgatta tcactgctgc attggcgctt tgctctaccc gtggtcctat gacgaaggtc
     7081 ccgaaatgcc gcgcgaacac gaagccgctt atcaggcgct cggcaatgcg atgcttaggc
     7141 cttttaaaga tgtcgggcgc tatcggttgg gcaaaactcc gaccttgctt ggctacaacg
     7201 cagtcggcac ttcaaaagac tactactatg cggtgcatgg tacgctcgcg ttcacgttcg
     7261 agggagtgtt tcgcaaagag caagccaatc ttgctcaaca cattgcgatg tgggagtctg
     7321 tctttcgctt cttgaacgca gaaaaagcgg cagaagcgga gtgggagtgg acttatggag
     7381 ctacatacta gtcggaccaa cggccccaat ccgggcgctg cgggcccaaa ggggcactgg
     7441 tttttcggta atttgctcga ccgtcgccgg cgaccgctcg aactctatgc tgatagcact
     7501 cgagtatatg gtaaaaccgt acgttttcgc atgggtccca aaaccgtatg gctgcttagt
     7561 aatccggtcg acgtgcagct ggttttgcac gatcaagcca agaaatttgt taaaggatac
     7621 ggctacgata ttcttgagat ttttttaggc cgaggactgt taacttcaga cggcagtttt
     7681 tggcaccgtc agcgccgact cattcagccc gtgtttcacc gatccaattt ggtgggtttc
     7741 gcgcaagtca tgcaggatga agcagacgcc cttgttctcc gcctgcgtgg acaggtcgaa
     7801 gtcgatgtaa ctacgaccat gctcaaactc accatggaaa ttgtcggcaa aacgcttttt
     7861 ggcacaaatg tgtcggacca tacttcggaa gttagcccaa ttatttctga attaatcgaa
     7921 gacgccaatc gccgtttgtt gtcggtcgta ccattgccgg tgtccgtgcc gctaccacgt
     7981 aacttgagaa ctcgccgact aattgagcgc cttgatggat tgatgtcgaa aatgatcgag
     8041 gagcggcgta atcaaaaaga gaacgatcca aaagacaaag gtatcgatct tttgagtttg
     8101 ttgattgccg ccactgacga ccaaaccaac gaacgcatga ctgatcgaca attgcgtgac
     8161 gaagccatga cgattttcgt ggctggacac gaaaccaccg caaatgcact tgcgtggaca
     8221 ctcatattgc tggcgcagca cccggaagtt caaacgcgtg ctcgcgagga agttcaaaaa
     8281 cctaatgcag cgccggggct ttttgagcgt gtgctttcgt acgaatatct ggggcgggta
     8341 atcgatgaat caatgcgcat gtacccgccg gcgtgggttg tttcgcgcca atcgaccgaa
     8401 ccagtagcgc tgtcgcaaac tgtcgtgcat ccagacgaaa ttgtcgcgat ttcaagttgg
     8461 gtgttgcacc gcgatcccga tttctggaaa gatccgcaaa catttgaccc agagcgtttt
     8521 acggcagacg aatgtgccaa aaggcacaag tattcctaca ttccgttcgg cgctggtcca
     8581 cgccaatgca ttggcacgca gttcgcgcgt gtggaactat tgaccgtgct ttcaacgctg
     8641 ctgcgaaact accgatttga actatcaggt ggttcgcaac agaaaataga accccacccg
     8701 ttggtcacac tgcgaccacg tggcagggtt cctatgaagt ttattccgat ttaaatgctt
     8761 atttgatgaa gtgcttgttc aaaaccgacg tcatcttcat catattgatg ggttcgccgc
     8821 cgattaacgg agcaagcgta gcgtcaggaa cgattgttcg cttgtcgttt gggtcttgaa
     8881 ggttgttttt cttaatgtaa tcccaaagtt ttttagtcac atcggctcgg gagatttcgt
     8941 cagctcctac aaactttgcg agctccggcg acacttttac cttcttgttg agtgcggatt
     9001 cttttgctgt catgtgtcta ggtcctttct aaaaattctg agaattaaca aaagtatttc
     9061 atcgatgtcc cgggttgtct acctgtcata agaacgataa aggcccggaa actttcgacg
     9121 taaaaactct gaaatatcat cgagtaccgc gatggccgac ggaaatacaa acgcggtgag
     9181 tagcgaacca aaagcgaggc cccaccccag tgccagcgca atcggaacaa caaaccgatc
     9241 taagccccca attccgtagg cagtgggtaa aaggccagcc acggtcgttg tagtggtcag
     9301 aaaaatcggg cgaattcgaa gcttggcagt ttgaataatg ctttcgaacc gatccattcc
     9361 attgatacgt cgttgattta caaagtcgac gaaaacgatc gcattattca cgatcacgcc
     9421 acccaatgcg ataatgccga gcatcgacat gaagctcaac ggcaagccgt gaaagaatag
     9481 tgtccaaatc accgaaataa taccgagcgg aagagtgatc aacaccaata ggggttgcag
     9541 tagctgtttg aaggtcatta ctaagatcag aaaaatggca aaaaaagcga ttccaaaggc
     9601 cctcatgagt gacttcaagc tttcctgagt gtcttcgtct tctccgccaa aggcgatcgt
     9661 tacattcgga aatttttggt tgaggtcagg aagcattgcc cgcacagcgt tatttgcctc
     9721 tagtgcgcta ctcttcgtta cgtcaacctc cgcagtgacc ttgacctggc gttgattggc
     9781 ttcgtgttca taaaccgcaa gcgactgagc ttcagagatt ctagccacgc tggcaagtgg
     9841 aacaagattg ccaaaagagt tgggtaccag aatttcgcga agtacatttt cttcgctgcg
     9901 ttgtttttcg agcaacgaaa ctcttacgtc aatttcttca tcgagattgc gtacggaagt
     9961 ggcaacaatt ccttcgaaag ccgcccgaac ggtggtgcca attccggcca cagtcaaacc
    10021 ggccgctgcg gcttcggtgg ggtttacggc tactcgtaat tcgggtttgc cgagcacgta
    10081 ggaatcgtta atgtctgtta cgccggtaac gcccgttaat aactgtttca gttcttttac
    10141 agccggcatg atatcttcgt aagttttacc gcgcacgccg atgctgactg gtttaccagt
    10201 cggcgggcca gggctaactc gctcgaagct cacgcgcacg aagtctttgg gtgttccgcc
    10261 tatttggcga gcagcctcga taatttcaat cgcctttcgt tttcgctcgg tctcgggcgt
    10321 caagaaaacc gtaatttggg catattcgct cccacgcttg gtgttgggat cgtttggatc
    10381 ttgttgttga atgccaatgc tagtgatgta atttttaact tcgttttttg gtaactgttc
    10441 gacaattttt tcgagctgtt tggtgaggcg ggccgtagtt tcgagactag aacccacttt
    10501 ggcttgagcg cgaatgaaaa atatttcaat gccctctggc ggaaacaaga taaaccgcat
    10561 tcccttgacc gctaaaccaa tgctactcga aaaaacaaaa accaacgcaa gcatcatgac
    10621 ccagcggtgg cgcagggtca cggtgagcaa cttaatgtag cgaggcacca cccagctttc
    10681 ccaaaaaccc cgggttttta aaagtgtgct gtcgagtttt tgccgaaccc gtgatcgact
    10741 cttgcgttcg cccccattgg cggcaaccga tggtacacga acgaatcggg caatgtgacc
    10801 gggaagaata aaaaaacctt caaacaaact aatgaggagc gcgcaaataa cgccaagcgg
    10861 aatttctcgt acgaatttac caaaaattcc actcatgaac atcatgggca aaaaggctac
    10921 gatcgtggtt agcaccgacg cggtgacagg ggccaccatt tgagaggcgc cttcgattgc
    10981 cgcagctcga ggttccatgc cctcttcgat aaggcgaact gaattgtctg tgacgacgat
    11041 ggcgtcatca accaacatgc cggaaacgat aatcaagccc agcattgaaa tgaggttaat
    11101 gctaaaaccc atccactgaa agatcagcat ggtgcccaaa aacgcaaaag gtattcccaa
    11161 tgaaacgatc aaagctaccc gtattggcaa aataagcgac agcaaaaaaa gtaccagtac
    11221 aagaccgatc atgaggttgc cggtgagaat atttagacgc cttcgtatga attccgacat
    11281 gtcgttgaga aaactcagct tcacacgcgg atctaggttt tttccaatct ggttcagttt
    11341 ggttttaaga ctgtcgacca gggtaatggc gtcggctttt tcttttttta ataccgtaag
    11401 accgaacgcc ggaagaccgt cagtgccgtt aatggtcgta gcgcgctcga gatcgtcgta
    11461 aactttagcg acatctccta cgcgcactcc ctgggcaagg tcattggcgc gaatcacagt
    11521 ttttcgaaca tcttctagag tgaggaagtc accgatagtg cgaacaattc gctcgtctct
    11581 ttccgggtgc tcggggtccg cctctacggt tccaccggga atagaaacgt tctgaccctt
    11641 gagcgatcga atcaaatcgt cgagcgagag tcggtagcga gcaagtttat tttgatctgc
    11701 ctccactcta atttctaagt cacgcaagcc tcgatgcact acgcgcgcaa ccccggggat
    11761 cgcttcaatt tcattttcaa gatgcttggc gactttgcga agttccaaat cggtcatcgc
    11821 tcccgacaca gacactt
//
LOCUS       DAOMXO010000007        11043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_3183_length_11043_cov_4.382963, whole genome shotgun sequence.
ACCESSION   DAOMXO010000007 DAOMXO010000000
VERSION     DAOMXO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 11043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11043
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3183_length_11043_cov_4.382963"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PLH57_00350"
     CDS             complement(<1..1350)
                     /locus_tag="PLH57_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01086.1"
                     /translation="MIVPRNLRLSLFAFTWLLLTPSKASGFLGELLHLPPNASYQQLQ
                     NERFSVVYPKEYEEEAKRTIVLLEEAHQKLAERFRYNRSDQVTVLLADTEDRANGVAS
                     AIGLQGLVLYLKAPEPYSPVGEYDDWLRMLVFHEYTHYLTLVQTRGLFSFLRVFLGHA
                     LLPNHAWPSWLSEGLAVWVESNYTRQGRGHGTYFNTITRDALARGDIDSDDFLGFDLI
                     SANPRPEFPFGDSAYYAGYGIVDELVKRNGPEALGTLVEEGSARIPYFLNGTLENVVE
                     KHVGTVAINEWRGLWNAWTSRERDRLGSELEWLKQRPSSELVTRTHSTEGDSAIGARL
                     SPNAELLAYSVQSGHHDYQLKLKNLRSGKVLLVLEGLSTPGVAFSEDSSKLYYSRLEY
                     VGEHRVHADLYEFEIARAKHKRLTHGFRAKDPDLCGDKLVYATQKGRFSRLETFDLYS
                     "
     gene            complement(1347..2267)
                     /locus_tag="PLH57_00355"
     CDS             complement(1347..2267)
                     /locus_tag="PLH57_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01087.1"
                     /translation="MKHHTYVFEFPHWEIVEVSGPDAKDFLHRITSADFRNYLGNGPL
                     SGTMLTALGKILFYFDAAPNARRDGYMLVSPRISESSAVVELEKLHFAENVTIRSARP
                     EWRFFRVLTNLNDLPNLFDRQITMELNQWTVDHWPLELKYPDAPTRDFGVLTNEPNVT
                     PQLASEDLSEYFRIASAFPASGLELDGSTIPLEAGLTKAVHENKGCYPGQEVIERIRS
                     IGQTARVLGRIRGSGTRPTKGMNIFGDTDSAAVVATLTSSAQAPNSSQWVGLAYFSRL
                     FAQGKKHAVGTLQNGAPVTIDFVDTGAKRA"
     gene            2336..3061
                     /locus_tag="PLH57_00360"
     CDS             2336..3061
                     /locus_tag="PLH57_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRK01088.1"
                     /translation="MNLSTLTWTEVESYLKTKNSLILPIGSTEQHGPTGLLGTDHLCA
                     EAVSAGVGAKLQMMVAPSVNYGMASHHMAFSGSATLRPSVYQAMLADIFRSFYAHGFR
                     RFYVVNGHGGNINSVGAVFQELKHEGMQGASFALYNWWKLPEVEAVSKKYYGDKDGFH
                     ATPTEVSLTFHLAGIDHRPFEETPQKGNAPWPMTAQEVRQVFPTGAMKSDPGLARGEH
                     GKVFLDLAVQSIANAIQQAAVIE"
     gene            3058..3972
                     /locus_tag="PLH57_00365"
     CDS             3058..3972
                     /locus_tag="PLH57_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRK01089.1"
                     /translation="MSVFEENVSNIRFIAWDTETTGLSPRIDEIVEIGAVAFDEDFEH
                     RVFESLIRPRMAIPTLVSSIHGITDSMVQDSPTPDVVLPQFVDFLTWSGQPRVLVAHN
                     ASFDVGMVHGEWIRQKTSSGRRGASDLLPSPEICIDSCQLARSLIDDAPRHSLEALAE
                     YFGISHASKHRAMGDVKVLKEVFLRLLGLASDRYADANGLRLERLIMLCGGYYELHTN
                     PASVSIELIKGAFKFSPALERISILCGGSSSIAICYDRDEEWRYVTPIGMKTKNSRVY
                     VEAFCHRDQMKKTFRGDRIRSVGKVLET"
     gene            4620..5444
                     /locus_tag="PLH57_00370"
     CDS             4620..5444
                     /locus_tag="PLH57_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRK01090.1"
                     /translation="MISRNVLLGAPLKLTPWRKLAVGSWKNVCEASIYSTLELEVDPV
                     LARIEELRQQTGEKITLTSVLVYAVAETMKNYPALNVLLRLGRLYPRKSVDIFVHVAV
                     DKRGDDLTGFVVRQADQKKITRIAQECAGAAAGVRGDGGRDAFSKTKKLFGLFPGFLA
                     RPLLGLSSFILYTLNLWSPLIGVPRDSFGSAMVTNVGALGISTAFAPIAPYTRVPLVI
                     AAGSTEKRAVVRDGAVRTVTILPLSVTIDHRVIDGLYAGLMSRRLKEIFSNPAALH"
     gene            complement(5441..6631)
                     /locus_tag="PLH57_00375"
     CDS             complement(5441..6631)
                     /locus_tag="PLH57_00375"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01091.1"
                     /translation="MEPTLKKLLSEVLRQPTAPFRETWTRAKFLHTCLEHSLKHWLDP
                     IGNLWLGVENLSEAKRTELLLVAHLDHPGFVVNRFFEKKTKHRTRRYAEAQWLGGGPM
                     EIRNFGVTIFSDFHAGVKFGGTIVEAEKAKRSIGRALIELEDSPERNPMISSIVAAPK
                     QSLRDLGPWGACLNYKECPDGVKFSNGECITKAADDLVGACAITAAFLDSNPARRVAA
                     CLTRAEESGFHGALEILNKKWLNPTRTKMISIETSAWRAGAELGKGPVVRQGDRVSLF
                     DHSLVEQIESMAIALKKKNKDFTYQKRVMDGGACEASAFRVFGFEVAGVSTPLGNYHN
                     IDPHLRAKPEIVRTNDVEGLYWLLCEIIENFSNAQFQRLKAKPLAQFGKEILRNHRAH
                     TKFF"
     gene            6663..8279
                     /locus_tag="PLH57_00380"
     CDS             6663..8279
                     /locus_tag="PLH57_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRK01092.1"
                     /translation="MTQQSAYEHLEEVARLMELAGENPFKVRAFQKGADAVRGTQEKL
                     ESIASGEVKVAGIGDGIRTTLGEFLKTGDSTARNELSAKVPAPILELLKLPGLGPKKA
                     RQVYEELGVQTLGELEYACRENRLLTLKGFGKSTQEKLLKNLELLKASRGKALLIEAL
                     DYAAEIETLFGGNSVRVGQLARHCEVVSLFEWHITDVPKKIHFEKKSEKKVFGMSLQK
                     LEFLHKELACVLWVSSAIDEKLRVWLNSSEDLRKRLEQSVALAHEVQDWEPSWLETTW
                     IGQTELRKPNAETYRVRRDGGVRGVFHNHTTESDGTASLEEMVTEAAKLGYQYIGISD
                     HSQSAFYAHGLSVDRIRAQRKAIASLQKRCGIRVFHGIESDILADGTLDYPDSVLAEF
                     DFVIGSIHSRFQMDEAAMTNRILRAMENPYMTFWGHPSGRLLLGREPYSLNWNQVFDA
                     AKDNGVIIEFNAHPSRLDIDWRFGRMLEEKEIQVSLNPDAHDLEGLAHVQLGARVLEK
                     AMLSKSLVFNLRSVEEVEKSLWERRKKAQS"
     gene            8249..8989
                     /gene="nth"
                     /locus_tag="PLH57_00385"
     CDS             8249..8989
                     /gene="nth"
                     /locus_tag="PLH57_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRK01093.1"
                     /translation="MGTKKKSSELSLQSRMLSVLKILKSTYPNAHCSLNYKNPYELVV
                     ATILSAQCTDDRVNKVTPNFFAAFPTVQQLANGKINEIERLIQSTGFYRNKAKSLLEC
                     AKMVIERHDGKIPRTLEDLVKLRGVGRKTANVVLGNAFGIPGLVVDTHVGRLSRRLGF
                     TRATDPVKVEHELERVVPKEDWVQFSHLLIYHGRARCMARNPDCAGCELSKLCEMRGV
                     SPATRKKVASRKPVLTRRALMRDLSTHK"
     gene            8986..9855
                     /locus_tag="PLH57_00390"
     CDS             8986..9855
                     /locus_tag="PLH57_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01094.1"
                     /translation="MKRLRAEKSIFNWSRVFLVLVLLGCNNQLIRQSPSTDLFSAVPE
                     GGTPSLDASLGFHPKRLWRLVLRWDPFMMDRKQAWVQKDFQLFIEDSRERQQFEKEIN
                     LIGGEEPYLAWLQTRLAPIGRSDRLLESRISRLRDSTIVSLIYRSENPVPIKGAFSVE
                     HVEFPGMPTAVRSLGCASNDESFMSESIEKDLNIAFEIVLHSRRIALLNNTYSAQVAE
                     SSLPLYASLWEQMRFRTDRVQVAERSHVLKDRVVYRTGIRISPPFAKCPIRPEDISLL
                     TKLRFWQPMGVEW"
     gene            9849..>11043
                     /locus_tag="PLH57_00395"
     CDS             9849..>11043
                     /locus_tag="PLH57_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01095.1"
                     /translation="MVKFFLQAAFVAQLVSFGLSIGGKRALAQDALSLSAVDQKANEA
                     MRELSDTLPYTPAALPLESLFAHAVEQLLVDNTFPKLRPVIRVVNLGGRDIFHIGGGK
                     VLLSGGVFREHPTEDSFIGRFSEVLAQAIVRDRYAPTGAPSIDVWRPHFEKWFFCRQK
                     EIRDLSTPDDGINFASNAEAVKEFERLRDKFSEKLRASGALHPYVGLRKAFLATPHQI
                     YGELCDDDRVFVAENFYKNSKWKELLDFCRAELRRRPKHELWRWLEAVAIANLGQPKV
                     CVSLTSSRWTHFDERRQHLNNACVGLSGNWSRAISQADEVAVARPRDFQARFYKAFLR
                     GRARLPIRDEITEIEKLWGDRPGLRALKVMYFAASEKPKEAENNVNFFPEFDQYEAWE
                     WGALAL"
BASE COUNT         2783 a         2492 c         2900 g         2868 t
ORIGIN      
        1 gctataaagg tcgaatgttt ctaacctaga gaatcgacct ttttgggtag cgtaaaccaa
       61 tttgtcgcca caaagatcgg ggtctttagc ccgaaatccg tgggttaagc gcttgtgttt
      121 agcgcgcgct atttcgaatt cgtaaagatc ggcatgtacg cgatgttctc cgacatattc
      181 caaccgagag taatataatt tactgctgtc ttcagaaaat gcgacacctg gagtcgaaag
      241 cccttcaaga acaagaagca cttttccaga gcgaagattt ttaagtttta gctgatagtc
      301 gtggtggccc gattgcacgg agtaagccag cagctcggca ttaggagaca gacgagcccc
      361 tatggcggaa tctccttcag ttgaatgtgt gcgagttacg agttcggaag agggtcgttg
      421 tttgagccac tccaattccg aaccaagccg atctcgttct cgtgaagtcc aggcgttcca
      481 aagaccgcgc cattcgttaa tcgccacggt tccgacatgt ttttctacta cgttttctag
      541 cgttccgttt aaaaaatagg ggatgcgagc ggaaccttcc tcgacaagag taccaagggc
      601 ctcggggcca ttgcgcttta cgagttcgtc gacgattccg tagccagcgt aatacgcgct
      661 atctccaaaa ggaaactcag ggcgcgggtt tgccgatatt aaatcaaaac ctaaaaaatc
      721 gtcgctgtcg atatcacctc gggcaagtgc gtcgcgagtg atggtattaa agtaagtgcc
      781 gtgtccgcgc ccctgcctcg tgtagttcga ctctacccac acggcgagtc cctcggacaa
      841 ccaagagggc caagcgtgat tcggaagaag cgcatgacca agaaacactc gaagaaacga
      901 aaataaaccg cgagtttgca cgagcgtcaa atagtgagtg tattcatgaa acactagcat
      961 tcgtagccaa tcgtcgtatt cgcccaccgg gctgtagggt tcgggcgctt tgaggtaaag
     1021 cactaaccct tgaagtccga tggcactcgc cacgccattg gcgcgatctt ctgtgtctgc
     1081 caataaaacg gtaacctggt cgctgcgatt gtaacgaaag cgttctgcta atttctgatg
     1141 ggcttcttca agtaaaacta tagttcgctt ggcttcttct tcatattctt tcggataaac
     1201 gaccgaaaat ctttcgtttt gaagttgttg ataacttgcg tttgggggca agtgcaaaag
     1261 ctcgccaaga aaaccactcg ctttgctagg ggtaagtaac aaccacgtaa aggcgaataa
     1321 cgacagtctt agatttcttg ggacgatcac gcgcgtttag ctcctgtgtc cacgaaatcg
     1381 attgtgaccg gcgccccgtt ctgcagggtt ccgacggcat gtttttttcc ctgcgcaaag
     1441 aggcgggaaa agtaagcgag gcccacccat tgcgaactat ttggggcctg agcgctactt
     1501 gttagtgttg ctacgacggc ggcgctgtca gtatcgccga aaatgttcat tcctttggta
     1561 ggccgcgttc ctgaaccgcg aattcggccc agtacccttg cggtttgccc aatggatcga
     1621 atgcgctcga tgacctcttg accaggataa caacctttat tttcatgcac agcctttgtt
     1681 aggcccgcct cgagaggaat agtggaacca tccagttcaa gaccggatgc cggaaatgca
     1741 cttgcgattc ggaaatattc cgaaaggtct tcgctggcaa gctgaggggt cacgtttggt
     1801 tcattggtca aaactccaaa gtcccgagta ggagcatcgg ggtatttaag ttcgagcggc
     1861 cagtggtcga cagtccactg gttgagctcc atggttattt ggcgatcgaa taaattcggc
     1921 aggtcgttca gattagtcag cacgcgaaaa aagcgccact ccggtcgggc cgatcgtatc
     1981 gtgacatttt ctgcaaaatg aagtttttcc agctctacca ccgcactgct ttcggaaatt
     2041 cgtggtgaaa caagcatata accgtcgcgt cgggcattgg gggctgcgtc aaaataaaat
     2101 aaaatctttc cgagggcagt gagcatggtg cccgacagtg ggccgtttcc caaatagttt
     2161 cgaaaatcgg ccgatgtgat tcggtgcaag aaatcttttg catcgggacc actgacctcg
     2221 acaatttccc agtgagggaa ctcaaatacg taggtatgat gcttcatatc ggaaaaccta
     2281 tgcgattcgt ggcactttga ccaggccatg ctataaccgg acccggaggc tcgctatgaa
     2341 tttaagcacc ttaacttgga ctgaagttga aagttatctg aagaccaaaa actccctgat
     2401 actacccata ggcagtacgg agcagcacgg gccgacgggt ttgttgggca ccgaccatct
     2461 atgtgctgag gcagtgagcg cgggggtggg agctaaactt caaatgatgg tcgcaccgag
     2521 cgtaaactac ggtatggcaa gtcatcacat ggcctttagc ggaagcgcta cattgcgtcc
     2581 gtcggtgtac caagccatgc tggctgatat ttttagaagt ttctacgccc atgggtttcg
     2641 tcgtttctat gtcgtgaatg ggcacggtgg aaatattaat agtgtcggtg cagtgtttca
     2701 ggaactcaaa cacgagggca tgcagggcgc atcgtttgcg ttgtataact ggtggaaatt
     2761 gccggaagta gaagcggttt caaaaaaata ttatggcgat aaagatggtt ttcacgcgac
     2821 tccgacagag gtttctttaa cctttcactt agcgggaata gatcatcgtc cgttcgagga
     2881 aacccctcaa aagggaaatg ctccgtggcc gatgactgct caagaggtac gccaagtatt
     2941 tccgaccggt gcaatgaagt cggatcctgg tctggcgcga ggtgagcatg gaaaagtgtt
     3001 tttggatcta gccgtacagt cgatcgcgaa tgccattcaa caggcggccg taatcgaatg
     3061 agtgtattcg aagaaaatgt ttcgaacatt cgatttattg cctgggatac cgaaaccaca
     3121 gggctttcgc cgcggatcga cgaaatcgta gaaattggag cggtcgcttt tgacgaggat
     3181 ttcgaacatc gcgtgtttga atcgcttatt agacccagaa tggctattcc aactctggtt
     3241 tcttcgattc acggaatcac ggactctatg gtccaagaca gcccgactcc ggacgtcgtt
     3301 ttgcctcaat ttgtagattt tttaacttgg agtggacaac cccgggtgtt ggttgcgcac
     3361 aatgcttcgt ttgatgttgg aatggtgcac ggagaatgga ttcgccagaa aacatcaagt
     3421 ggccggcgtg gtgcttcgga tttattacct tcaccagaaa tatgcattga ttcctgccag
     3481 ctagcgaggt cgttgatcga cgatgcgcct cgccacagcc ttgaagcgct cgcagaatat
     3541 tttggaattt ctcatgccag caaacaccgg gctatgggtg acgtgaaagt tttaaaagaa
     3601 gtgtttctcc gactattggg ccttgcttca gatcgttatg ccgacgcaaa tgggctgcga
     3661 cttgaacgcc tgattatgct ttgtggggga tattacgaac tgcatacgaa ccctgcatct
     3721 gtttctatcg aactcataaa gggtgccttt aagttctcac cggcgttgga acgcatctcg
     3781 attctgtgtg gcggttccag ttcgattgct atttgctatg atcgtgacga agagtggcgt
     3841 tacgtgaccc caattggaat gaaaacgaaa aattcgcgag tttatgtaga agccttttgt
     3901 caccgtgatc aaatgaaaaa gacatttcga ggcgatcgca ttcgatctgt ggggaaggta
     3961 ctggaaactt gaaaaattat tcattgataa aaaaatggac aataattttg gggttaggct
     4021 tgatcagcag tttaacgagt catgcgcaaa cgagcccgtt ttcttgggag cagtgttcgg
     4081 gaacagcaaa ggcagttggg tgtggactga cgttttatgt cgatcagtgt cgcgctcgtt
     4141 tcgaattacg gtggccagaa ctggcgatcc ccggcactag tgaatggaat cgcgataaac
     4201 gcaattggtg tacgctttct tggtaccgag gggaaaaaag aattcaaacc cacaaacaga
     4261 cagccaaagg atgtgttcgg atatttcaat tgttctccga tgctgttcga aactttcccg
     4321 aagtgccgat gcagcaaagc ctatgttcat tagcttcccc gagcgaggga ctattagtgg
     4381 tatcagagtc caagaataaa aaggccaata tggtccctca tgtcggtcgg tgtcaggtcg
     4441 aaacttcgaa aagtcgcagc ccaattcaat taaattgtcc acttgtggat gatcgaaccg
     4501 aagtttggtt aaacgaactt ggacgtcagg cgaagataac cgtgcgattt tacaatccag
     4561 gggaccaagg tccattgagc acaccacccc cgaatcgagt attacgctga ggtttagaaa
     4621 tgatttcacg taacgtgttg ctcggagcgc ctttaaaact aactccatgg cgcaaactag
     4681 cagtcgggtc ttggaaaaat gtatgtgagg ccagtatcta ctctacgtta gagttagaag
     4741 tggatccggt attggcgaga attgaagaac tccgacaaca aacaggcgaa aaaatcactc
     4801 ttacttcggt cttggtttat gcggtcgccg aaactatgaa aaactatcct gcgcttaacg
     4861 ttttgctacg actcggtcga ctttaccctc gaaaatcagt tgatattttc gttcatgtcg
     4921 cagtcgacaa aaggggagat gatctgactg gcttcgtagt tcgccaagcg gatcaaaaga
     4981 aaataactag aatcgcgcaa gagtgtgctg gggcggcggc tggtgttcgc ggcgatggtg
     5041 gccgcgatgc tttttcaaag accaagaagc tttttggtct ttttcctggt tttctagcgc
     5101 gcccgctttt gggactttca agctttatac tgtacaccct taatctatgg agtcccttaa
     5161 ttggagtacc gcgagactcc tttgggagcg ccatggtgac caacgttggg gcccttggaa
     5221 tctctactgc ctttgctccg atcgcccctt acacgcgggt tccattagtg atcgccgcag
     5281 gttctaccga aaaacgagca gtagttcgcg acggagctgt ccgaacggta accattcttc
     5341 cgttaagtgt gaccatagat catcgggtga tcgacgggtt gtacgcagga ctgatgagtc
     5401 gtcgacttaa ggaaattttt agtaatcctg cagcgctaca ctagaagaat ttcgtgtgcg
     5461 cgcggtggtt tcgcaaaatt tcttttccaa attgagccag cggttttgct ttcagtcttt
     5521 gaaactgcgc attggaaaaa ttctctatga tttcacaaag tagccaataa aggccttcta
     5581 cgtcatttgt tcttacgatt tctggtttag ctcgcagatg tggatcaata ttgtggtagt
     5641 ttccaagtgg cgtcgacacc cccgcgactt cgaaaccaaa aactcgaaac gcagaagctt
     5701 cgcaggcccc accgtccatg acgcgctttt gataagtgaa gtctttattt ttctttttaa
     5761 gcgcgatcgc catagattca atctgttcta ctaaggagtg atcgaaaaga ctcactcggt
     5821 cgccttggcg tactacaggg cctttgccga gttcagcccc cgctcgccaa gcgctcgttt
     5881 caattgagat cattttcgtt cgcgtgggat ttagccactt cttgttcaga atttcgaggg
     5941 ctccgtgaaa tccggactct tcagcccttg ttaaacaggc tgctacgcgt cgtgccgggt
     6001 tagaatctaa aaatgccgca gtaatggcac aagctccgac caaatcgtcg gctgccttgg
     6061 tgatacactc gccgttgcta aacttaactc cgtcggggca ttccttatag tttaggcacg
     6121 caccccaggg gccgaggtct cgaaggcttt gtttgggagc tgcaacgata ctcgatatca
     6181 ttgggtttct ctcgggcgaa tcttcgagct ctatcaacgc gcgaccgatc gaacgtttgg
     6241 ctttttcggc ttctacgata gttccaccaa acttaactcc ggcgtgaaaa tccgaaaaaa
     6301 tcgtaacccc aaagttcctg atctccattg ggccgccgcc caaccactgg gcctcggcat
     6361 aacggcgtgt tcggtgcttg gtttttttct cgaaaaacct attaactacg aaaccgggat
     6421 gatccaggtg cgcaaccaat aggagttctg tgcgtttggc ttcagacagg ttttccacgc
     6481 ctaaccaaag gttaccgatc gggtccagcc agtgttttag ggaatgttca agacaggtat
     6541 gtaggaactt tgcgcgggtc caggtttcgc gaaaaggggc ggtaggttgt cttagcactt
     6601 cgctaaggag cttttttaga gtgggttcca tatttcggta ctttcgcata tcttaaatgc
     6661 caatgacaca gcaaagcgcc tatgaacacc tcgaagaagt tgcgcgactt atggaacttg
     6721 cgggggagaa cccgttcaaa gttcgggcct ttcaaaaggg tgccgacgct gtgcggggta
     6781 cccaagaaaa actcgagtcc attgcctcgg gtgaagtgaa agtcgcggga attggtgatg
     6841 gaattagaac gaccttgggg gaatttttaa aaactggaga ttcgaccgct agaaacgaac
     6901 tgtcggctaa agtgccggca cctattttgg aacttttgaa gttgccggga cttgggccga
     6961 aaaaagcacg gcaagtatac gaagaactcg gagttcagac acttggggag ttggaatatg
     7021 cctgtcggga aaatcgactt ctgacgctca aagggtttgg caagagcacg caagaaaaat
     7081 tgctaaagaa tttagaactt cttaaagcct cacgaggcaa agccttgtta atcgaagcgt
     7141 tggactacgc ggctgaaatt gagaccttgt tcgggggtaa ctccgtgcga gtggggcagt
     7201 tggcccgcca ttgcgaggtt gttagtttat ttgagtggca cattactgac gtacctaaaa
     7261 aaattcattt cgaaaaaaag tcggaaaaaa aagtttttgg catgtctctt cagaaacttg
     7321 aatttcttca caaagagctc gcgtgtgtgc tttgggtttc gagtgctatc gacgaaaagc
     7381 tgcgcgtttg gttgaattct tcagaggacc tgcgaaaaag gctggaacaa agtgttgcac
     7441 tagcccacga agttcaggat tgggagccta gttggcttga aacaacctgg attggtcaaa
     7501 cggaattgcg taaacccaat gctgagacct atcgagtgag gcgtgacgga ggagtgcgag
     7561 gagtttttca caatcacact accgaaagtg acggtacggc cagtctcgaa gaaatggtta
     7621 ctgaggcagc aaagctcggg taccaataca tcggaatttc ggatcacagc cagtcggcgt
     7681 tttacgcgca cggtttgtcg gtagatcgca ttcgagcaca acgaaaggcg atcgcttctt
     7741 tgcagaaaag gtgcggcatt cgagtgtttc atggcatcga gagtgacatt ttggccgacg
     7801 gaacgctcga ttacccggac tcggttcttg cggaattcga cttcgtgatt ggttctattc
     7861 attcgcgatt tcaaatggac gaagcggcaa tgacaaaccg aattctccga gcgatggaaa
     7921 atccatacat gactttttgg ggccacccat cgggccgttt gctactcgga cgcgagccct
     7981 atagtttgaa ttggaaccag gtgttcgacg ccgcaaagga taatggagta ataatcgagt
     8041 tcaacgccca ccccagtcgc ttggatatcg attggcgatt tggccgaatg ctcgaagaaa
     8101 aagaaattca agtttctctt aatcccgatg cgcacgatct tgaggggttg gcacatgtgc
     8161 agctcggtgc gcgcgtatta gaaaaggcta tgctgtcaaa gagtttggtt tttaatttga
     8221 gatcggttga ggaagtggag aaaagtttgt gggaacgaag aaaaaaagct cagagctaag
     8281 tttgcagtcg cgcatgttga gcgttctaaa aattctaaaa agtacttatc ccaatgcgca
     8341 ctgttcgttg aattataaaa acccctatga gttagtggtg gctacaatat tgtcggcaca
     8401 atgcactgat gaccgcgtca ataaagttac accaaatttt tttgctgcat ttcccacggt
     8461 tcaacaattg gccaacggaa aaattaacga aatcgaacgc cttattcaaa gtactggatt
     8521 ttaccgcaat aaagcaaagt cactgctcga gtgcgcgaaa atggtgatcg aacgtcacga
     8581 cggaaaaatt ccacgcacac tggaagattt agttaaactg cggggagtgg gtcgaaaaac
     8641 tgcgaacgtt gtgttgggaa acgcatttgg aattcccggc ctggtggtcg acacacacgt
     8701 gggtcgtctg agccgccgtc tcggttttac tcgagcgact gatcccgtaa aagttgaaca
     8761 cgaattggag cgtgttgtac ccaaagaaga ctgggtacag ttttctcatt tactaattta
     8821 tcatgggcga gcccgttgca tggcgcgtaa cccggattgt gctggttgtg aactatcgaa
     8881 actctgtgag atgcgcggag tgtcgcccgc aactagaaaa aaagttgcgt ctcgtaaacc
     8941 agttctcaca cgtcgtgcat tgatgcgaga tttgagtacg cacaaatgaa acggttgaga
     9001 gctgaaaaat cgatttttaa ttggtcgcgt gttttcttgg tgctcgtctt gttaggttgc
     9061 aataatcaac ttattcgtca gagtccgagt accgatcttt ttagcgccgt tccagaaggt
     9121 gggacaccct ctctcgacgc gtctttgggg tttcatccta agcggttgtg gcgattagtg
     9181 cttcggtggg atccttttat gatggaccgc aagcaggcgt gggttcaaaa agactttcag
     9241 ctgtttattg aagattcccg tgaacgccaa cagttcgaga aggaaattaa cctgatcggc
     9301 ggagaagaac cgtatctggc gtggcttcag actcgtcttg ctccaatcgg tcgatcggat
     9361 cgattgctcg aatctagaat atcgcgcctt agagactcca ctattgtctc gctgatctac
     9421 cgctcggaaa atccggttcc gataaaaggc gcgttttcgg tagagcacgt agagtttccc
     9481 ggaatgccga ctgcagtgcg atcactgggt tgtgcgagca atgatgaaag ttttatgagc
     9541 gaaagtattg aaaaggattt gaatatcgca tttgagattg tgttgcattc gaggcgaatt
     9601 gcgcttttga acaataccta tagcgcccag gttgcagaga gctcattgcc tctttacgcg
     9661 agtttgtggg aacaaatgag gtttcgaacc gaccgggttc aggtagccga gcgatctcac
     9721 gtacttaaag atcgagttgt ttaccgaaca ggcattcgta tttcgccacc ctttgcaaag
     9781 tgtccgattc gaccggagga catttcgctt cttacgaagc tgcgtttctg gcagccaatg
     9841 ggggtagaat ggtaaagttt tttttgcagg cggcttttgt cgcgcagcta gttagtttcg
     9901 gactatcgat aggcggtaaa cgcgcattag cgcaggacgc tctttcattg agtgccgtgg
     9961 atcaaaaggc aaacgaagcg atgcgtgaac taagcgatac gttgccctac acgccagcag
    10021 cgctcccact cgagagtctt tttgcacatg ccgtcgagca gctcttagtg gataatactt
    10081 ttccgaaatt gaggccggtt atccgcgtcg ttaaccttgg tggtcgcgat atatttcata
    10141 ttggcggcgg aaaagtatta ctttcgggcg gtgtttttcg agagcaccct acggaggatt
    10201 cgttcatagg gcggttttcc gaggttctgg ctcaagcgat tgttcgcgac cgttatgcgc
    10261 cgactggggc gccttcgatt gatgtgtggc ggcctcattt tgagaaatgg tttttttgtc
    10321 ggcaaaaaga gatacgagat ttgtctaccc cggacgatgg tattaatttt gcctcgaatg
    10381 cagaggccgt aaaggaattc gaacggttgc gagataagtt ttcagagaaa ttgcgggcgt
    10441 ctggggcact gcacccatat gtagggctcc gtaaagcgtt tcttgctaca cctcaccaaa
    10501 tttacggtga actttgtgac gacgatcgcg tatttgttgc cgaaaacttc tacaaaaata
    10561 gtaagtggaa agaactgtta gacttttgtc gcgccgaatt acggcggagg ccaaaacacg
    10621 aactgtggcg gtggctcgaa gccgtcgcga tagctaattt gggtcaacca aaagtgtgtg
    10681 tatcgctcac gagtagtcgt tggacccatt tcgacgagcg acgccagcac ttgaacaatg
    10741 cgtgtgtggg tctttctgga aattggtctc gagccatttc gcaggccgac gaagtcgcgg
    10801 ttgcacgacc tcgcgatttt caggcccgtt tctacaaagc gttcctacga ggacgcgctc
    10861 gattaccgat tcgcgatgaa attaccgaaa ttgaaaaact ctggggcgac cgcccaggac
    10921 ttcgcgctct taaagtaatg tattttgcag cgagcgagaa acccaaagaa gcagaaaaca
    10981 acgtgaactt ttttcctgaa tttgaccagt atgaagcttg ggaatgggga gcgttggcac
    11041 ttg
//
LOCUS       DAOMXO010000008         9377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_3879_length_9377_cov_4.727848, whole genome shotgun sequence.
ACCESSION   DAOMXO010000008 DAOMXO010000000
VERSION     DAOMXO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3879_length_9377_cov_4.727848"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3020
                     /gene="rpoB"
                     /locus_tag="PLH57_00400"
     CDS             <1..3020
                     /gene="rpoB"
                     /locus_tag="PLH57_00400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRK01096.1"
                     /translation="NSPEEALIEIYKRMRPGDPPTLEAARQLFDNMFFNPDRYDLSKV
                     GRLKLNYKFPNNGISPDVTVLTKTDIMETVRYLCELNNGRGTIDDIDHLGNRRVRAVG
                     ELIENQYRIGLVRMERAIRERMSIQEVETLMPHDLINQKPVSAVVKEFFGSSQLSQFM
                     DQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPIETPEGPNIGLIA
                     SLSTYARVNDFGFIETPYRQVKERKVHEEIRYFTATEEENKIIAQTNPEVLKAGRFDA
                     NAAPARRKGEFALVNPDELELMDVSPNQLVSIAASLIPFLEHDDANRALMGSNMQRQA
                     VPLLRTAAPVVGTGIERVVAKDSGSIVASRHDGEVTMVDGTKIVVRRTNAKNDEIGSD
                     VDIYNLTKYQRSNQNTCVTQRPIVKAGDRVKAGDILADGPATDGGELALGQNVLVAFM
                     PWQGYNFEDSILISERVIRDDTFTSIHIEEFECVARDTKLGKEEVTRDIPNVGEEALK
                     DLDESGIIRIGAEVKPGDILVGKVTPKGETQLSPEEKLLRAIFGEKAGDVRDTSLRVP
                     SGIFGTIISAQVFAREGTDPDERTKQILEDEVAKLRRDEKIEIDAVRLSAQAKIYKML
                     DGEKTTGKLLSEDGSEELLKKGEDLNKATLAKIPFELLAYIPVKDELEKEVTTFMKTA
                     KEKIDAVKAIFKERSDKLKRGDELAPGVIKMVKVYVAIKRRLSVGDKMAGRHGNKGVI
                     SRIVPQEDMPYLADGTPVDMVLNPLGVPSRMNIGQILEVMLGWAGRALGDKLQTYAAK
                     FQPEQLRKLIKEVYSSPDVDKYIDKASEAELKRIVYSLRKGVHFATPVFDGASEKDIH
                     KMLTIAEVPTRGQTTLYDGMTGDAFSEDVTVGVMYMLKLHHLVEEKIHARSIGPYSLV
                     TQQPLGGKAQFGGQRLGEMEVWALEAYGAAHTLQEMLTVKSDDVTGRNRMYEAIVKGE
                     HVLEPGLPESFNVLVKELQSLALNVELIEDKQE"
     gene            3045..7184
                     /gene="rpoC"
                     /locus_tag="PLH57_00405"
     CDS             3045..7184
                     /gene="rpoC"
                     /locus_tag="PLH57_00405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRK01097.1"
                     /translation="MKDLLNFFDKPKDPLSFTGIRISLASSEKIREWSFGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECNCGKYKRMKHRGVVCEKCGVEVIQSKVRRERL
                     GHIELATPVAHIWFLRSLPSRIGALLDVTLKDLEKVLYCETFVMIDGGNSEVESGTII
                     SEEKYNQLIKDGATFTAGMGGEAVRDLLKKIDVDLMGRQLRKEMRATSSEAVRTKIAK
                     RLKVVEALKESGNRPEWMMLEVVPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNN
                     RLKRLMELNAPEIIIRNEKRMLQEAVDALFDNGRRGKTFTGPSKRPLRSLSDMLKGKQ
                     GRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKQMALELFKPFIYNKLSEYGYVTT
                     IKSAKKMVEKQRQEVWDILDEVVKEHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHP
                     LVCSAFNADFDGDQMAVHVPLSIEAQIEARVLMMSTNNILSPAHGKPIIVPSQDIVLG
                     LYYMTRERPFAKGEGKVFASSTEVTYAYASDAMHLQAKIKCRVGKKLYDTTVGRVLLS
                     EVVPPEIPFQYYNRVLDKKALAELIDHCYRLAGNKKTVILADQLMATGFKMATRAGIS
                     ICVDDMLMPNEKEEFLKKAYSQVQEIENQYVDGLITDGERYNKVIDIWAQTSEVIASA
                     MMKKLSTQKIRGPKGEEVEVPSFNGIFMMADSGARGSNAQIRQLAGMRGLMAKPSGEI
                     IETPITANFREGLSVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVSQDMIIREV
                     DCGTLDGIYVSALIEGGEIIERMGDRILGRVALDDVIDPITSEVIVSANTEIVEDKVR
                     KIDDAGLDRVKIRSVLTCQSADGTCTLCYGRDLARGRLVNIGEAVGVIAAQSIGEPGT
                     QLTMRTFHIGGAASRRAEQSNLEVKTKGRIKFLNVTTVKNKEGHFIAMNRNGEVAVID
                     DNGREREKYPVIYGARLLAKDGDKVEKGKMIAEWDPYSTPIVSEATGTVKFEDIEEGL
                     TMQEQVDPVTGLSHKVIVESKASDKRPKILIQDDKGQLKNIEGSHRVARYMIPVGANV
                     VVLDGDQVHAGDVIAKIHRETTKTKDITGGLPRVAELFEARKPKDAAIISEIDGIVSF
                     GKDTKGKRKIVVTPEVGEPKEYLVGKGKHLAVNEGDYVKAGDPLMDGPINPHDILRVK
                     GEKALAKYLVDEVQEVYRLQGVKINDKHIEVIVRNMLRKVKIQDVGDTTFLVGEQVDK
                     QVFEEENQKALAQNGTPATAEPLLLGITKASLSTESFISAASFQETTKVLTEASIAGK
                     VDYLRGLKENVIMGRLIPAGTGLAKYKRIIVESTAETVDQPVTSSLMHQPSASADATV
                     M"
     gene            7259..7639
                     /gene="rpsL"
                     /locus_tag="PLH57_00410"
     CDS             7259..7639
                     /gene="rpsL"
                     /locus_tag="PLH57_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRK01098.1"
                     /translation="MPTINQLVRKGREKKVWKTSAPALEGCPQRRGVCVRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIPGIGHNLQEHSIVLVRGGRVKDLPGVRYHTVRGTL
                     DTQGVQNRKQSRSKYGAKRPSKAS"
     gene            7668..8138
                     /gene="rpsG"
                     /locus_tag="PLH57_00415"
     CDS             7668..8138
                     /gene="rpsG"
                     /locus_tag="PLH57_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRK01099.1"
                     /translation="MSRKKFIAKREILPDPKFNDLVVAKFINSLMVDGKKSVAEGILY
                     STLDILQKRTNDDPLKVFKKALENLKPVLEVKSRRVGGATYQVPVEVKPARRQSLASR
                     WLIEFARDRAEKSIEEKLASEVLDALANRGGAMKRKDDVHRMAEANKAFAHFRW"
     gene            8263..>9377
                     /locus_tag="PLH57_00420"
     CDS             8263..>9377
                     /locus_tag="PLH57_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRK01100.1"
                     /translation="MAHIDAGKTTTTERILYYTGKTHKLGEVHEGTATMDWMPQEQER
                     GITITSAATTCFWKNHRINIIDTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSE
                     TVWRQANKYKVPRVAFINKMDRIGADFWSCVDQMKEKLGANAIPFQLPWGVESEFKGV
                     IDLISMKGVFWQGENLGASFEESDIPNELLEEAQSAREALVGAVAEFDEQCMEKYVNG
                     EDIPVQDLRRAARKACVSLGVTPVFCGSSFKNKGVQPLLDAVINYLPSPLDIPPVAGH
                     DVKNHDKIIERKADYKEPFAALAFKIMTDPFVGTLTYFRVYSGTLEAGSHIYNSIKEK
                     KERIGRLLQMHANKREDVKEARAGEIVAAVGLRFTTTG"
BASE COUNT         2678 a         1986 c         2411 g         2302 t
ORIGIN      
        1 tgaactctcc cgaagaggct ttgatcgaaa tttataagcg catgcgtcca ggcgatcctc
       61 cgacactaga ggcggcccga caacttttcg acaatatgtt cttcaatccg gaccgctacg
      121 atctttcgaa ggttggccgt ttgaagctca attataaatt cccaaataac ggaatatctc
      181 cggatgtcac tgttttgaca aaaaccgaca tcatggaaac tgtgcgctac ctttgtgaat
      241 tgaacaacgg tcgcggtact atcgacgata ttgaccatct tggaaatcgt cgtgttcgcg
      301 cagtgggcga gttaatcgaa aaccaatatc gtattggtct ggtgcgtatg gagcgcgcca
      361 ttcgtgagag aatgagcatt caagaagttg aaacgctcat gccgcacgac ctcattaacc
      421 aaaaaccagt ttcggctgtg gttaaggagt ttttcggttc gagccaactt tcgcagttca
      481 tggaccaaac gaacccgctt tctgagatta cgcacaagcg tcgtctttcg gcactcgggc
      541 cgggcggtct cactcgtgaa cgcgcgggct ttgaagtccg tgacgtacac ccgactcact
      601 acggtcgaat ttgtccgatc gaaacgccag aaggtccaaa cattggtctt attgcgtctc
      661 tatcgacata tgcacgcgtg aatgattttg gttttatcga gacgccttat cggcaagtaa
      721 aagagcgtaa agttcacgaa gaaattcgct attttactgc gactgaagag gaaaataaaa
      781 tcattgcaca gaccaatccg gaagtattga aagccggcag gttcgacgca aatgctgctc
      841 cagcacgccg aaaaggcgag tttgctttgg ttaaccctga tgaactcgaa ttgatggacg
      901 tcagtccaaa tcaattggtt tctatcgcgg catctttgat tccatttttg gaacatgacg
      961 acgccaatcg cgccctgatg ggttccaaca tgcaacgtca ggcggtgcct ctcttgagaa
     1021 cggccgcccc agttgtgggt acaggaatcg aacgcgtggt tgctaaggat tcaggttcca
     1081 tcgttgcttc gcgccacgat ggtgaagtta cgatggtcga tggcaccaag atcgtagttc
     1141 gccgtacaaa tgcgaagaac gacgaaattg gttccgatgt agatatctac aatctcacca
     1201 agtatcagcg atctaaccaa aacacttgtg tcactcagcg tccgattgtg aaggctggcg
     1261 atcgcgtgaa ggctggggac atcctggcag atggtcctgc tactgacggt ggcgaattgg
     1321 cccttgggca aaacgtgttg gtcgcgttca tgccgtggca gggttataac ttcgaggact
     1381 cgattttaat tagtgaacgg gtaatcagag atgacacgtt cacgtccatc catatcgaag
     1441 aattcgagtg cgttgcccga gatactaagc tcggaaaaga agaagtaact cgagatattc
     1501 caaatgtagg cgaggaagca ctaaaagacc tcgacgagag tggaattatt cgaattggtg
     1561 cggaagtaaa gcccggcgat attcttgtag gaaaagtcac acctaaaggt gagacccaac
     1621 tttctccaga agagaagcta ctgagagcta tctttggcga aaaagccgga gacgtgcgcg
     1681 atacatcgct tcgggtacct tcaggtatct tcggaacaat catcagcgcg caagtatttg
     1741 cacgcgaagg aactgatccg gacgagcgca ccaaacaaat cttggaagat gaagtggcga
     1801 aattgcgtcg agacgagaaa atcgaaatcg atgcggttcg cttgagtgcg caggcgaaga
     1861 tatacaagat gctcgacggc gaaaaaacta ccggaaagct cttgagcgaa gatggttccg
     1921 aggaattgtt gaagaagggt gaagacctta ataaagcaac ccttgcaaaa attcctttcg
     1981 aattgcttgc atacattcca gtcaaagacg agcttgaaaa agaagttacg acttttatga
     2041 aaactgcaaa agagaaaatc gatgcagtaa aagcgatctt caaggagcgc tcggataaac
     2101 tgaaacgcgg agacgagctt gctcctggcg tgatcaagat ggttaaggtg tacgtagcta
     2161 ttaagcgtcg tcttagcgtc ggagataaaa tggcgggacg ccacggaaac aagggcgtta
     2221 tctccagaat tgttccgcag gaagacatgc cgtatttggc agacggaacc ccagtagata
     2281 tggtgttgaa ccctcttggt gttccttcac gtatgaatat tgggcagatt ttggaagtga
     2341 tgcttggttg ggcaggtcgc gcgcttgggg acaagttgca aacctacgca gctaaatttc
     2401 agccagaaca gttgagaaaa ctaattaaag aagtttactc gtctcccgat gtcgacaaat
     2461 acatcgataa ggcaagtgaa gccgaactta agcgaattgt ttatagtttg cgaaagggtg
     2521 tgcactttgc gacgccagtt ttcgatggcg ctagcgaaaa agacatccac aaaatgttga
     2581 ctattgcgga agttccgact cgtggacaaa caactttgta tgacggcatg acaggcgacg
     2641 ctttctctga agacgtaact gtaggtgtta tgtacatgtt gaaacttcac catctggtag
     2701 aagaaaagat tcacgcaaga agtattggac cttactcgct tgttacgcag caaccgcttg
     2761 gtggtaaagc ccagtttggt ggccagcgtt taggagagat ggaagtttgg gcacttgaag
     2821 cctacggcgc cgctcacaca cttcaggaaa tgctgacagt gaaatcagac gatgtcactg
     2881 gtcgtaaccg aatgtatgaa gcgattgtta agggtgaaca tgtacttgaa ccaggtctcc
     2941 ctgagtcctt caacgtactt gtgaaagaac ttcaaagctt agctctgaac gtagaattga
     3001 tcgaagacaa gcaagaatag tttgtttttt taggagaaaa tctcgtgaaa gaccttctta
     3061 atttttttga taaaccgaaa gacccattga gctttacagg catccgaatt tccctggcat
     3121 cgtcagagaa aattcgcgag tggtcctttg gtgaagtaaa aaagccagaa accatcaact
     3181 accgtacgtt taagcctgaa cgcgatggtc tgttctgtgc caaaatcttc ggaccagtga
     3241 aggactatga gtgtaactgc ggtaagtaca aacgcatgaa acaccgaggc gttgtgtgtg
     3301 aaaaatgcgg cgtcgaagtt attcaatcaa aggttcgtcg tgaacgtctc ggtcatattg
     3361 aacttgcgac tccagtagcg cacatctggt ttttaagaag tcttccgtcc cgtattgggg
     3421 ctttgttgga tgtgacgtta aaagacctcg aaaaggttct ttattgcgaa acattcgtaa
     3481 tgatcgatgg tggtaacagc gaagtcgaat ctgggaccat tatttccgag gaaaaataca
     3541 accaactcat taaagacggc gctactttta ccgccggaat gggtggcgag gccgtacgcg
     3601 atcttttgaa aaaaattgac gttgatctca tggggcgtca acttcggaaa gaaatgcgcg
     3661 caacttcgag cgaggcagtt cgtactaaga ttgcaaagag acttaaagta gtcgaagcac
     3721 tcaaagagtc gggtaatcgt ccagagtgga tgatgttgga agtagttcca gtaatccccc
     3781 ctgagttacg cccattggtt ccacttgatg gtggtcgttt cgctactagc gatctcaacg
     3841 acctttatcg tcgtgtaatt aatcgtaata atcgtctgaa gcgcctcatg gagttaaacg
     3901 ctccggaaat tatcattcga aatgaaaaac gcatgctgca agaagccgta gatgcgttgt
     3961 ttgataatgg tcgacgaggt aaaacgttca caggtcctag taagagacca ctacgttcgc
     4021 tttcggacat gcttaagggc aaacaagggc gtttccgcca gaacctgctt ggaaaacgcg
     4081 tcgattattc gggtcgttcg gtaatcgtgg ttgggccaga attaaagctt catcagtgcg
     4141 gtttgccaaa gcaaatggcg ctcgagttat ttaaaccttt tatctacaac aagctttctg
     4201 agtacgggta cgtaacgacc attaagagtg cgaaaaagat ggtcgagaag cagcgtcagg
     4261 aagtctggga cattctggac gaagtggtta aggagcaccc tgtactcttg aaccgtgcgc
     4321 ctacgttgca tcgattgggc attcaagctt ttgaaccggt gttaatcgaa ggaaaagcga
     4381 ttcaacttca tcctctcgtt tgttcggcat ttaacgctga ctttgacggt gaccagatgg
     4441 ccgtacacgt gccgttgtcg atcgaagctc aaatcgaagc gcgcgtcctt atgatgtcga
     4501 ccaacaatat tctttctcca gctcacggaa aaccgattat cgttccgtcg caggatatcg
     4561 tactaggtct ttattatatg acccgcgagc gtccgttcgc aaaaggcgaa ggcaaagtat
     4621 tcgcttcctc gacagaggtg acatacgcct acgcttcgga tgcaatgcat ttgcaggcga
     4681 agatcaaatg ccgcgtcgga aagaaattgt atgacacgac cgtaggtcgg gtgctcttga
     4741 gcgaagtcgt tcctccggag atccctttcc aatactacaa ccgtgtattg gataagaagg
     4801 cattggctga gttgatcgac cattgttacc gtcttgccgg caataaaaag actgtgattc
     4861 tggctgacca gttgatggct accggattca agatggcgac tcgtgcggga atttcgatct
     4921 gcgttgacga catgttgatg ccgaatgaaa aggaagaatt tctcaagaag gcctactccc
     4981 aagttcaaga aatcgaaaac caatatgttg atggtctcat tacagatggt gagcgttaca
     5041 acaaggtgat cgatatttgg gctcagacct cagaagttat tgcatccgca atgatgaaga
     5101 agttgagtac acaaaagatt cgtggaccaa agggagaaga agtcgaagtt ccttctttca
     5161 acggaatttt catgatggcg gattctggag caagaggtag taatgcccaa attcgtcagc
     5221 tcgcaggaat gcgtggcctc atggcgaaac cgtcaggcga aattatcgaa actccgatta
     5281 ctgctaactt tcgtgaaggt ctaagtgttc ttcagtactt tatttcgact cacggagcgc
     5341 gaaaaggctt agcggatact gctcttaaaa cagcaaattc cggttatctt actcgtcgtc
     5401 tcgtggatgt gtcgcaagac atgattatcc gagaagttga ttgtggaacc ctagacggaa
     5461 tttacgttag cgccttaatt gagggcggcg aaattatcga acgcatgggc gaccgtattt
     5521 tgggtcgtgt ggcgcttgac gatgtgatcg atccgatcac tagtgaagtg attgtctccg
     5581 cgaacactga aattgtggaa gataaagttc gtaagattga cgacgcgggg ctagaccgag
     5641 tgaagattcg ttccgtgctg acctgtcaat ctgcagatgg tacttgcaca ttgtgttacg
     5701 gtcgagacct tgcacgcgga cgccttgtga atatcggaga ggctgtcggt gtaattgctg
     5761 cacaatctat cggtgaacca ggaacgcagt tgacaatgag aacgttccac atcggtggag
     5821 ctgcgtctag acgtgcagaa caatcaaacc tcgaagttaa aacgaagggg cgaattaagt
     5881 ttctcaacgt aaccacagtg aagaataaag aaggtcactt catcgcaatg aatcgaaatg
     5941 gtgaagttgc ggtcattgac gacaacggtc gtgaacgtga aaaatatcca gtaatttacg
     6001 gtgctcgcct cttagctaaa gatggcgata aggtagaaaa aggaaaaatg atcgcagaat
     6061 gggatcctta ttccactccg atcgtttccg aagctactgg tactgtgaag ttcgaagata
     6121 ttgaggaagg tcttaccatg caggagcagg tagacccggt caccggactt tcccataagg
     6181 ttatcgtaga atcgaaggca tcggataaac gtccgaaaat cttgattcag gacgataaag
     6241 gacaattgaa aaacattgaa ggtagccacc gtgttgcgcg ctacatgatt ccggtgggag
     6301 ctaacgtagt agttctcgac ggggatcaag tacacgcagg cgatgtcatc gcgaaaattc
     6361 atcgtgagac aacgaagacc aaagatatca ccggcggtct tcctcgcgtt gcggaactct
     6421 ttgaagctcg caaaccaaag gatgctgcaa ttatttcgga aatcgatggc atcgtatcat
     6481 ttggtaagga caccaagggc aaacgtaaga ttgtagtgac tccagaagtc ggtgagccga
     6541 aggagtactt ggtaggcaaa ggaaaacact tagccgtcaa cgaaggcgac tatgtgaagg
     6601 ccggagatcc tttaatggat ggaccaatta atccgcatga tattttgaga gtaaaaggcg
     6661 aaaaagccct tgcgaagtat ctcgtggacg aagtgcaaga ggtctatcgc ttgcagggcg
     6721 tgaaaattaa cgacaaacac atcgaagtga ttgttcgtaa catgcttcgc aaagtaaaaa
     6781 ttcaggacgt tggcgatact acattcttgg tcggcgaaca ggtcgataag caggtgttcg
     6841 aagaagagaa ccaaaaagca ttggctcaaa atggaacgcc agcaacggca gagccgctat
     6901 tgctcggtat taccaaagcc tcgttaagta ctgagagctt catttcagca gcttccttcc
     6961 aagagaccac taaggttctc acggaagcga gtattgcagg aaaagtcgat tatttgagag
     7021 ggttgaagga aaacgtcatt atgggacgcc taattccggc tggtacggga ttagcgaaat
     7081 ataagaggat tattgttgaa tctacggcgg aaaccgtaga ccaaccagta acatcctcgt
     7141 tgatgcacca accaagtgct tcggcagacg ctacagttat gtaaattcgc aaccttgact
     7201 cgacttattt gatcgagtac aaggctgctt catagtttag gaagttgaaa ggaacagaat
     7261 gcctactatt aatcagttgg ttcgaaaggg acgagaaaaa aaggtttgga aaacgagcgc
     7321 gcccgcgttg gaagggtgtc cacaacgtcg tggtgtttgt gtgcgggttt atacaaccac
     7381 accaaaaaag ccaaattccg ctttgagaaa agtttgtcgg gttcggttaa ccaacggatt
     7441 tgaagtgact tcctatattc ctggcattgg ccacaatctc caagaacact ctatcgtgct
     7501 tgttcgaggt ggtcgtgtga aagacttgcc aggcgtacga taccacaccg tacggggaac
     7561 tctcgatact caaggtgttc aaaatcgtaa acaaagccgt tcgaagtatg gcgcaaaacg
     7621 cccaagtaag gcttcttaag aaagaatttc agaggagtag attttaaatg tctcgtaaaa
     7681 agttcatcgc aaaaagagaa atccttcccg atccaaaatt taacgattta gtcgtagcga
     7741 agttcatcaa ttcattgatg gttgatggga agaagagcgt ggctgaagga attctttatt
     7801 ccacgttaga tatacttcag aagcgaacta acgatgatcc actgaaggtg tttaaaaagg
     7861 cattggaaaa tttgaagccg gttttagaag taaagtcacg tcgcgtaggt ggagctacct
     7921 accaagtgcc agtagaggta aaacccgctc gccgtcaatc tttagctagt cgttggttaa
     7981 tcgaatttgc tcgagatcga gcagagaagt cgattgaaga aaaattggca tcggaagttt
     8041 tagatgcgct tgctaaccgc ggtggtgcaa tgaagcgtaa ggacgacgtt catcgcatgg
     8101 ctgaggccaa taaagcgttt gctcactttc gttggtaatc gagctttttt caacgagctt
     8161 ggtcgaatgt ttgcggctgt acttttcgga gtacggccgc ttttttttta ggggaagtcc
     8221 gtggctgact acaacttaga gtctactcgc aatatcggca ttatggccca cattgatgcc
     8281 ggaaaaacga cgactaccga gcgaattctt tattacacag gaaaaaccca caaattgggg
     8341 gaagttcacg agggcaccgc aaccatggat tggatgcctc aggaacaaga acgcggaata
     8401 acgattacct cggctgcgac cacatgtttt tggaaaaacc atcgtattaa tattatcgat
     8461 actcccggcc acgttgattt cactatcgaa gttgaacgaa gcttgcgcgt attagatggc
     8521 gcagtcgcag tgttttgttc agttggcggc gtagagccgc agtcggaaac cgtttggaga
     8581 caggctaaca aatacaaagt ccctcgagtt gcatttatca acaaaatgga ccgcatcgga
     8641 gccgatttct ggtcgtgtgt cgatcaaatg aaagaaaagc ttggcgcgaa cgcaattccg
     8701 tttcaactcc cttggggagt cgaaagcgaa ttcaaaggtg taatagatct catctcgatg
     8761 aagggtgttt tttggcaggg agaaaatcta ggagcctcgt tcgaggaaag tgacattcct
     8821 aacgaacttc ttgaagaagc acagtccgca agagaggctc tggttggagc ggttgccgaa
     8881 ttcgatgaac agtgcatgga aaagtacgtc aatggcgaag acattccggt tcaagatttg
     8941 cgacgtgcgg cccgaaaggc ctgtgtgagt ctaggtgtta cgccagtgtt ttgcgggtcc
     9001 tcctttaaaa ataaaggagt tcagccactt ctcgacgcag tgataaatta tctaccttcg
     9061 cccttggata ttccgccagt tgctgggcac gacgtaaaaa atcacgataa aattattgaa
     9121 cgaaaggccg attataaaga gccgtttgct gcacttgcgt tcaaaataat gacagaccca
     9181 ttcgtaggta cgttgaccta ttttcgcgtc tactccggaa cgctcgaagc tggttcacat
     9241 atttataact cgatcaaaga aaagaaagag cgaatcggcc gtttactcca aatgcacgcg
     9301 aacaaaagag aagacgtaaa agaagcccga gccggagaga ttgttgcagc cgttggtctg
     9361 agatttacga ccacggg
//
LOCUS       DAOMXO010000009         9206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_3960_length_9206_cov_5.328926, whole genome shotgun sequence.
ACCESSION   DAOMXO010000009 DAOMXO010000000
VERSION     DAOMXO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3960_length_9206_cov_5.328926"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PLH57_00425"
     CDS             complement(<1..779)
                     /locus_tag="PLH57_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRK01101.1"
                     /translation="MPKQTPLLAVEFLQHLRLERGLSTATTEAYANDLRGYFQFLDQT
                     KESLVNVSPVIANEYVTALQKRNYAPASIHRKISAVTGYYDFLVREQKCMINPFTKVE
                     YAPHRALPTVPPSQESIGELLEGDPSRVTSMALMFRLLYACGLRVSELVGLTLEAVDF
                     ERGVLRVTGKGEKTRLVPVDSGTLALLRRYTQTTRSAVARDSSQTKSFFLNPRGTSYT
                     RQGVWKALKKIAPGLSPHTLRHAFATHCLENGMNLRSLQLL"
     gene            966..1763
                     /locus_tag="PLH57_00430"
     CDS             966..1763
                     /locus_tag="PLH57_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01102.1"
                     /translation="MFKIIASATLLAAFVGCASVQPLDQIAARAKQPRGFANEVVVDT
                     SSRSVGGIKKLAWAGYTVTLECEAKCNLTREEQRKIAEQLASQSFESFDRELRANFAS
                     SAEEIQIQEVNETTSATFFAQEIRTESLTSRIGKWFEKLKLNHEPPVSVSPSGLKTLN
                     PNEIGWSGETSLQKLGQALGVDGVLVGHVKVMVSGEGESDSALLEIDGPKLWVFSSKR
                     ASGIAAARLRQSWSPHTADRTTLDLNGIDVVAKAYSEQFVRELRTIR"
     gene            complement(1902..2462)
                     /locus_tag="PLH57_00435"
     CDS             complement(1902..2462)
                     /locus_tag="PLH57_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01103.1"
                     /translation="MKRKWNTVLTLLAAISITLAGVGCGSNSEEENLNPNGVGAPGVY
                     GGGVHTLPGGVSVVTFNGTNVYDSGVTVYGTNYSTGYAGQNSISFGSPALPIGQPGTI
                     VSNSFIFPGTQLQLVKTPGSSTNYSNLSAVLNLSSAFTSTHGQISNLQMIDFRFYPQG
                     SSGAFKIYLCCATNPMTGQSSTVVIE"
     gene            complement(2647..3822)
                     /locus_tag="PLH57_00440"
     CDS             complement(2647..3822)
                     /locus_tag="PLH57_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01104.1"
                     /translation="MKNITRIALGFVFVIFLVKSFDYVVRHGDRLHDQDTGSEDNTIT
                     AARNFVQDGFFKSCGLPKYGRYNLDSASKTIAESDYYTHYFPGPDYVLATTSAVFGAH
                     FKTAQWSRLVPLFTFFLAVLFFLWVGARSLWPNQPLATPWIAAFILFAPANRNWVLNL
                     HGHGYVSAYTLAALSLGVLAASWTWSNRKNHFSIYGSMVGAFLLGFASNYMLLTNAFV
                     VCAAPLVGGLLAHSPNRKAWQLPFWLSFVVGLGLVAAFFVHLYQIACLFSWKDALADQ
                     LGTLSARVQKSHAAAPISRIRLLGQYSDQVFGFYGLSAKAMALLGLFLAWISSASGIG
                     RKRQIAALALAILAAYAWVLVLPSHASGHLHVNPRIFLLPFVCVLVLLARITGVSKK"
     gene            complement(3819..5027)
                     /locus_tag="PLH57_00445"
     CDS             complement(3819..5027)
                     /locus_tag="PLH57_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRK01105.1"
                     /translation="MNLRRQKLLDRGIGIPLLAILKPLVILAGIILKRDHTLPDPGLD
                     RGGRITVLKMLGGGSLVIALPALLGLRKRFPRVHFQLVTTPSVAPFARTLGVFDEILT
                     IEQNSVFALAASALHALVKCFRSNCFIDLEVYSKLSTVFSVFTCAHNRIGFYLENGIW
                     RKPIHTHLIFFNRFAPVHLFYERVAILLGAEVATYQECQKLLRSVCKIPEVSASPQHG
                     RPVVVIGSGCSALGQERMFTAPEWSQIFRSQLEKNPALSDSRIVFLGGPEDQTLADSI
                     LKILYASHPNLEIENLCGKLALTDSIRVLAEAREFWGIDSSLLHYARLLGRKCISFWG
                     PTAPFSRLKKFPIEEEVHYRKVPCSPCVHVTEFPPCNGNNVCMKSLVSPMTDDILEKN
                     LPVFHGAPEF"
     gene            complement(5032..7560)
                     /gene="mutS"
                     /locus_tag="PLH57_00450"
     CDS             complement(5032..7560)
                     /gene="mutS"
                     /locus_tag="PLH57_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRK01106.1"
                     /translation="MAQTTPLMQQYNEIKSRHQDSLLLFRMGDFYELFGTDAVEAAKI
                     LDIALTTRNKNDPTALQMCGVPHHALQNYLPKLIAAGKKVALCEQLENPSEAKGIVRR
                     DVVRVISPGTQLDPEVLDRTRPNLTHAALTRSDGSVYWATCDFSTGHFWFGATKTLTD
                     WELFLKTLAPSEVILPQSPNGGARNKVKELLPSAYVQEVPEFYFDEQYGRDRLSEQFG
                     AIHTDAINPKLSQNNSHIGPCAALIKLFQETQKTQRIPAVIRLVYWDEHQFMDLDPQT
                     IAALDLLPSDRTRGKSLYEFLNRTQTSMGARTLREWLLRPLIERSTIVERQRFVKSLY
                     LHTESVSQVLREIYDIERLLTRVSNAAEFGTSNARDLKALAQSIEQSWNLALALEPCS
                     PSLANRLQVRLSQDLRVLARETTANIVDLPPPTTREGGMFRKGFNSELDELIDLTERG
                     EQWLVQYEIELRQTTNISSLKVRFNRVFGYYIEVTKTNLKNVPDSFIRKQTMATAERY
                     ITPELKEFEDKILNAEKRRTDFEHALYRKQCSQFAAHAHPILETARAVAEIDALQTLA
                     RLLGEGGYVTPEIFNDTRLVIEAGVHPTVAHAMQSSGQSFVPNDLTMGSGNLPSFLLI
                     TGPNMGGKSTVMRQTALIVLLAQIGAPVPATRAELGIVDRIFTRIGSADNLAEGASTF
                     MVEMSEMSFILRQATTRSLILVDEIGRGTSTYDGLSLATAIAEHIVTRIGARTLFATH
                     YHELTLLESRVSGVQNVRVGVRVTSDANVEFLYRLEIGASERSYGILVAKLAGLPENI
                     LMRAQNVLEGHETGQSSLTKESASPVTVRLRRPAKTETHQLGLF"
     gene            complement(7638..>9206)
                     /gene="pyk"
                     /locus_tag="PLH57_00455"
     CDS             complement(7638..>9206)
                     /gene="pyk"
                     /locus_tag="PLH57_00455"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRK01107.1"
                     /translation="STALGKPVAILQDLQGPKLRVGRFPNGSITIEKGEEVLLKLENR
                     ETIAKQSSKIKVIPYNYERLSKEIQTGHRILMDDGNLEVVVTNIKNTDLTAQVVFGGT
                     LKDHKGMNFPDTRLTIEAFTPKDKEDLLLGLKLGVDYVALSFVRSAFDVRTVQRFMHA
                     HRKNIPVISKIEKAEAITNLPDILEVTDAVMVARGDLAVEVGTAQVPSLQKKIILECN
                     KRGIPVITATQMLESMIHTPRPTRAEASDVANAVLDGTDAVMLSAESASGKFPVLTVS
                     VMQNIIVETESKSQTKRNATLAYISHQLLSLQSFDHLPVVEAIERAASELAESVGAKL
                     VACTTHTGQAARALAKFRPVVPIIAFTDNVDVERRLALVWGVETIPAPSAVGLDELFQ
                     LAEVELVKRGYAKREDFVVFTAGYPPFRHGSTSVIKIIQLKTEAPDGKPKKPKDQNDN
                     LETYRTRKAQFLIDHALCIQCGGCVEVCPNDIFAYSGRKVYLKESNCKLCTFDNACME
                     ICPTSAIEIVKLSD"
BASE COUNT         2268 a         2158 c         2504 g         2276 t
ORIGIN      
        1 aacagcaact ggagcgaacg caagttcatg ccgttttcta aacagtgagt agcgaacgca
       61 tggcgtaacg tatgcggaga aagcccgggg gcaattttct taagggcctt ccatacaccc
      121 tggcgagtgt acgacgttcc tcggggattt aagaaaaatg attttgtctg agagctgtcg
      181 cgcgccaccg cactgcgagt ggtttgggtg tagcgccgta gcagcgcgag cgttccactg
      241 tcgactggta cgagtcgggt tttttcgccc ttgccggtaa ctcgaagaac gccccgctca
      301 aaatccaccg cttcgagcgt gagccctacc aattcgctca ctcgaagtcc gcaggcataa
      361 agcaaacgaa acatgagcgc catactggtc actcgactgg gatcgccctc caaaagctct
      421 ccgatcgact cctgggatgg gggaaccgta ggcagcgcgc gatgaggcgc atactccact
      481 ttagtaaaag ggttgatcat gcatttttgt tcgcggacta agaagtcgta gtacccggtt
      541 acggccgata ttttacggtg aattgacgcg ggcgcgtagt ttctcttctg caatgcggtt
      601 acgtattcgt ttgcaatcac cggactaaca ttcaccaagg actcttttgt ttggtccaaa
      661 aactgaaaat atccgcgcaa gtcgttagcg taggcctcgg tcgtagcggt gctgagaccg
      721 cgttccaaac gcaggtgttg gagaaattcg actgctaaaa gtggcgtttg cttgggcatg
      781 gttactattt taatgccaac aaaggcgggc tcgagaagag cgaggaagtt ttttcccctc
      841 ctgagcccag cgcgcctatc taagttgccg cggagttgcg cgctgaacgg gttttcgtta
      901 ggatgtttta cgcgcaaggc cgttgcgcag ggaccattga aaccgtggct cgggggatac
      961 aaattatgtt taaaattata gcgtctgcga ctcttttagc ggcattcgtt gggtgtgctt
     1021 cggtgcaacc gcttgatcaa attgcggcgc gtgccaaaca accgagggga ttcgcaaacg
     1081 aagtggtggt cgatacgagt agtcgctcgg tgggtgggat caagaagcta gcctgggctg
     1141 gttatacggt gacacttgag tgtgaagcca aatgtaacct gacgcgagaa gaacagcgaa
     1201 agatcgcaga acagttggcg tcgcaaagct tcgaatcgtt tgatcgtgag ttgcgagcaa
     1261 atttcgcttc cagtgccgag gagattcaaa ttcaagaggt aaatgaaacc actagcgcta
     1321 ctttttttgc ccaagaaata cgtacggagt cgcttactag cagaattggc aaatggtttg
     1381 aaaaattgaa gctcaatcac gagccccccg taagtgtgtc gccgagtggt ttgaaaacgc
     1441 tcaatcctaa tgaaatcggt tggtcaggcg aaacaagcct gcaaaagctc gggcaagcat
     1501 tgggggttga tggtgttttg gtcggccatg taaaagtgat ggttagcggc gaaggcgagt
     1561 cggattctgc attgttggaa atcgacggtc caaaactatg ggtgttctcg agcaaacgag
     1621 cgtcgggaat tgcggccgcg cgtttaagac aatcatggag tccacatacc gcggatcgta
     1681 ccacgcttga tttgaatggg atcgacgtag tggcgaaagc ctacagcgaa cagttcgtgc
     1741 gtgaattgcg cacaatccgc taaaaatcag gaagggacca gagtttttta ctctagtccc
     1801 cgttgtcctc tctagatgca cgttccgctt aatgccttgt ggcttaggct tcaacattca
     1861 ctctgcatgc caccagctct actactgcgg ttcgaaagtt tttactcgat gactactgtc
     1921 gacgactgac ccgtcatagg atttgttgcg caacataagt agatcttaaa tgcgccgctg
     1981 gacccttgtg ggtaaaatcg aaagtcgatc atttgcagat tgcttatctg accgtgggtc
     2041 gaggtaaatg cgctcgacag gttgagtacc gccgagaggt tcgaataatt ggtcgaacta
     2101 cccggagttt ttacgagttg caactgcgtg cctggaaaaa tgaacgaatt cgatacgatc
     2161 gttccgggct ggccgattgg cagggccggg gatccaaagc tgatgctgtt ttggccagcg
     2221 tatccggtgg agtaattcgt gccataaacc gttacgccac tgtcgtaaac gttggttcca
     2281 ttgaaagtaa ccaccgatac gccacctggt aaggtgtgta cgccgccacc atagacgccg
     2341 ggagcgccga cgccattagg gtttaaattt tcttcttctg aatttgaacc gcatcccacg
     2401 ccggcgagcg taatcgagat tgccgccaac aatgttagaa cggtgttcca cttccgcttc
     2461 atttctactt cctccaaaag agagttcatg catgatgcac aactctcgca aagttctctc
     2521 ggatgggttg aattgcaacg ggggtgccaa gttgacacag gacccatgtt cggcggcggt
     2581 ccctcgaaaa agtcgaaaat tagggggaaa ataggctagt taggagtgag gggcccgaac
     2641 aggtgtctat ttctttgaca ccccggtgat tcgagccagt aaaaccagca cacacacaaa
     2701 cggcagcaaa aatattcgag gattcacgtg taagtggcct gacgcgtgtg atgggagcac
     2761 cagtacccag gcataggccg ccaatatggc cagtgcaagc gctgcgattt gccgttttcg
     2821 gccaataccg ctcgccgaag atatccaggc gaggaacagg ccaagcaatg ccattgcctt
     2881 agcgctgagg ccgtaaaagc cgaatacttg gtcgctgtac tgacctagca gtcgaatacg
     2941 agaaataggt gcggcggcgt ggcttttttg aacgcgtgcc gaaagagtcc ccagctgatc
     3001 ggctagggcg tccttccagc taaaaagaca agcgatctga tacaagtgaa caaaaaatgc
     3061 tgccaccaaa ccgagtccaa ccacgaaaga aagccaaaat ggcaactgcc aggctttgcg
     3121 gttcggacta tgagcaagta gtccaccgac cagcggtgcg gcgcaaacca cgaacgcatt
     3181 tgtcagcaac atgtagttcg aagcaaagcc tagtagaaaa gctccgacca tcgaaccata
     3241 aatagagaaa tgatttttac gattactcca ggtccaactg gcggccagga ctcctagtga
     3301 aagcgcggca agagtgtaag cgctaacgta gccatgccca tgaagattta gtacccaatt
     3361 acgattggca ggggcgaaaa gtatgaacgc ggcaatccac ggagtggcga gcggttggtt
     3421 cggccaaagg gatcgagccc ccacccagag aaaaaaaagc accgccagga agaatgtaaa
     3481 aagtggaact aagcgcgacc actgcgctgt tttaaagtgg gcgccaaata ctgccgaggt
     3541 cgtggcgagc acgtagtctg gtcctggaaa gtagtgcgtg tagtaatcgc tctctgcaat
     3601 tgttttagaa gccgaatcta agttgtaacg tccgtatttc ggcagaccac aacttttaaa
     3661 aaaaccatcc tgcacaaagt ttcgggctgc ggttatggtg ttgtcctcag aaccggtgtc
     3721 ttggtcatgc aagcgatcac catgtcgaac gacgtagtcg aacgatttta ctagaaatat
     3781 aacgaataca aagcctagtg cgattcgggt gatatttttc aaaactcagg tgctccgtga
     3841 aatacgggta gatttttttc aaggatatcg tcagtcattg gcgaaaccaa acttttcatg
     3901 cacacattgt tgccgttaca tggtgggaac tcggttacgt gcacgcaagg agagcagggc
     3961 actttacggt agtgcacttc ttcttctatt gggaactttt ttagtcgcga aaacggcgcg
     4021 gttggtcccc aaaaactaat gcacttgcgt cctagcagtc gggcgtaatg gagtagagag
     4081 gaatctatcc cccagaattc acgagcttct gcgagcacgc gtatggagtc agtaagggcg
     4141 agttttccgc aaaggttttc aatctccaag tttgggtggc tcgcgtataa aatctttaga
     4201 atagaatcgg ccaatgtctg gtcttctggt ccgccaagaa aaactatgcg ggaatcgctt
     4261 agtgccgggt ttttttctag ttgggatcta aatatttgac tccactcggg agcggtaaac
     4321 attcgttctt gacccaaagc agaacagccg gacccaatta ccactaccgg acgaccgtgt
     4381 tggggggagg ccgagacttc gggaattttg cagaccgagc gcagaagttt ttggcactcc
     4441 tggtaggtgg ctacttcggc gccaaggaga atggccacgc gttcataaaa aagatgcaca
     4501 ggcgcaaatc ggttaaagaa aataagatga gtgtgaatag gttttcgcca aatgccgttt
     4561 tccaggtaaa aaccgatacg attgtgagcg caggtgaata ccgaaaatac ggtggagagt
     4621 ttgctgtaaa cttcgagatc aataaaacaa tttgaacgaa agcatttaac gagtgcgtgc
     4681 aacgcactag cggcgagtgc aaaaacacta ttttgttcaa tggtcagaat ttcatcgaac
     4741 accccaagag tgcgagcgaa cggagcgacg gagggggttg tcaccaattg aaaatgaact
     4801 cgcggaaatc tttttcgcag ccccagcaag gccggaaggg cgatcaccag actaccgcct
     4861 cctaacattt ttaataccgt gatccgacct ccacgatcta gacccgggtc cggaagcgtg
     4921 tgatctcgtt ttaaaataat gcccgcaaga attaccagtg gtttaagaat ggctaaaagc
     4981 ggaataccga ttccgcgatc aagcagtttt tgtcgacgga gattcatgga attagaacaa
     5041 ccccagttgg tgagtctcgg tctttgccgg acgacgaagt cgcacggtca ccggggacgc
     5101 ggattctttt gtaagcgaac tctgccccgt ctcgtggccc tctaaaacat tctgtgcgcg
     5161 cataagaata ttttcaggga gtcccgcaag tttggcaacg agaatcccgt aggatcgttc
     5221 cgatgcaccg atttccaggc gataaagaaa ctctacgtta gcgtcagaag taacacgcac
     5281 acccactcga acgttctgca cgcctgagac tcgggattcg agcagtgtga gttcatggta
     5341 atgggtggcg aacaacgtgc gtgccccaat tcgagtgaca atgtgttcag cgattgcggt
     5401 ggcaagactc agtccatcgt aggtgctggt gccgcgtcca atttcatcga ccaaaatcaa
     5461 actgcgagtc gtggcttggc gcagaataaa actcatctcg ctcatttcaa ccataaaggt
     5521 tgaagcgcct tctgccagat tgtccgccga tccgatgcgc gtaaaaatac gatcgacgat
     5581 tcccaactca gctcgagtcg cgggcaccgg cgctcctatt tgggcgagca gcacgatgag
     5641 tgcagtctgc cgcatgacgg tgcttttccc tcccatatta ggtccggtaa tgagtaaaaa
     5701 tgaaggaaga tttcctgagc ccatcgtcag atcgttcgga acgaaagact gcccagagga
     5761 ttgcattgcg tgagccactg ttgggtgcac gcctgcttcg ataacgaggc gagtgtcgtt
     5821 aaaaatttcg ggggtaacgt atcccccttc acctagcaag cgcgcaagcg tttgaagtgc
     5881 gtcaatttcc gccactgcac gagccgtttc taagattggg tgcgcatgag ccgcgaactg
     5941 actgcactgt ttgcggtaga gtgcatgttc aaagtcggta cggcgttttt ccgcattaag
     6001 aatcttatct tcgaattctt ttaactcggg cgtaatatac cgctcggcgg ttgccatggt
     6061 ttgtttacgt atgaaactat ctggaacatt cttgagattg gttttcgtaa cttcgatgta
     6121 atacccaaac acgcggttaa agcggacttt gagactcgaa atattagttg tttggcgcag
     6181 ttcaatttcg tactgcacga gccactgctc cccgcgttcc gttaggtcga tgagctcgtc
     6241 tagttctgaa ttgaaacctt tgcgaaacat accgccttca cgagtggtcg gcgggggaag
     6301 gtctacgata ttagcggttg tctcgcgggc caatacgcgt aggtcttggg aaagccgaac
     6361 ttggaggcgg ttggctagcg agggtgaaca cggctcgagc gcgagggcga ggttccacga
     6421 ctgttcaatc gactgggcaa gcgccttgag atcgcgggca ttcgaagtgc caaattctgc
     6481 ggcgtttgaa actcgagtga ggagtcgttc gatatcgtaa atttcgcgta acacctgact
     6541 taccgattcg gtgtgtaagt acaaggactt tacgaagcgt tggcgttcaa caattgtcga
     6601 acgttctatc aaagggcgca atagccactc tcgtagtgtg cgcgcaccca tagatgtttg
     6661 ggtacgattg aggaattcgt aaagactttt acctcgagtg cggtcggaag gaagaagatc
     6721 caaagccgcg atcgtttgtg gatcgaggtc catgaattgg tgttcgtccc agtacaccag
     6781 acgaataacg gcaggtatgc gttgagtctt ttgggtttct tggaacaact taattagcgc
     6841 cgcacatggg ccgatatggc tgttattttg ggaaagcttc ggattaatcg cgtccgtatg
     6901 aatggcacca aattgttcgc ttaaacgatc gcgcccgtat tgttcatcga aataaaactc
     6961 tgggacttct tgtacgtaag ccgacggcaa gagttctttt actttgttgc gagcgccacc
     7021 attgggggac tgtggcaaaa tgacttcgct aggtgcgagg gtttttagaa acagctccca
     7081 atcggtgagg gttttggtag ccccgaacca gaaatgcccc gtgctgaagt cgcaggtcgc
     7141 ccagtaaacc gacccgtcgg agcgggtcag tgccgcatga gtaaggttag ggcgcgtacg
     7201 gtctagcacc tcggggtcga gttgggtgcc tgggctgatc actcgtacca cgtcgcgccg
     7261 aacgattcct tttgcttcgc tcggattctc aagctgttcg cagagggcca ctttcttgcc
     7321 ggctgcaatg agttttggca aatagttctg tagcgcatgg tgaggcacac cgcacatctg
     7381 aagggctgtg gggtcgttct tgttgcgtgt ggtgagggca atatcgagaa ttttggccgc
     7441 ttccaccgcg tcggtcccga agagctcgta aaaatccccc atgcgaaaaa gcagaaggga
     7501 gtcttggtgg cgactcttga tttcgttgta ctgctgcatg agcggagtgg tttgggccat
     7561 gagaaacatt ttctaccgag aaccacccag cccggcaagg gagaatgggg ggtataatgc
     7621 ttagcgttat atttatgtta gtcgctcaat tttacgattt caatcgcgga ggtgggacag
     7681 atttccatgc acgcgttgtc gaaggtgcat agcttgcagt tactttcttt gaggtaaacc
     7741 ttacggcccg aataagcgaa tatgtcgttc ggacaaacct caacgcaccc cccacactga
     7801 atgcacagcg cgtggtcgat tagaaattgg gcttttctag ttcgataggt ttctagattg
     7861 tcgttttggt ctttaggctt tttgggtttt ccgtcgggag cctcagtttt taactgtatg
     7921 atcttgatga cgctagtgga gccatggcga aaaggcggat aaccggccgt aaacaccacg
     7981 aagtcttctc ttttagcgta tccgcgtttg acgagctcta cttccgcgag ctggaacaat
     8041 tcgtcgagtc caactgcact cggagccgga atagtttcca cgccccagac gagtgccaaa
     8101 cgtcgttcaa cgtcgacatt gtcggtgaaa gcgatgattg gaaccacggg gcgaaattta
     8161 gctaatgcgc gagcagcttg gccggtatga gtcgtgcagg cgacgagttt ggcaccgacg
     8221 gactctgcaa gttcagacgc cgcccgttca atggcttcaa ctacgggcaa gtgatcgaaa
     8281 gactgaagac ttaagagctg gtgcgaaatg taggctagag tggcattgcg cttggtctga
     8341 gatttcgact ccgtttccac gattatattt tgcattaccg aaaccgtgag tactgggaac
     8401 ttacccgatg cgctctcggc agaaagcatc accgcgtctg ttccgtccaa tacagcgttg
     8461 gctacgtcgc tcgcctcggc tcgagtaggg cgaggtgtat gaatcatgct ctccagcatt
     8521 tgggtggcgg tgatcacggg aattccccgc ttgttgcatt cgaggattat ttttttctgt
     8581 aggctaggca cttgagcggt tccaacttct actgcaaggt ctcctcgcgc gaccatcact
     8641 gcgtcagtga cttcgagaat atctggtaag ttggtgatcg cctcagcttt ttcgattttg
     8701 gaaataacgg gaatattttt tcggtgggcg tgcatgaacc gttgaacggt tcgaacatcg
     8761 aaggctgaac gtacaaacga aagcgctacg taatcaactc ccagttttag tcccaggagc
     8821 aggtcttctt tgtctttagg cgtaaaagct tcgatcgtta agcgagtatc gggaaaattc
     8881 atgcctttgt ggtctttgag agttccaccg aatacgactt gtgcggtaag atctgtgttt
     8941 ttgatatttg tgacaaccac ttctaggttt ccgtcgtcca ttaggatgcg atggccggtt
     9001 tgaatttctt ttgaaagtcg ttcgtagtta taggggatga ctttgatttt ggaactttgt
     9061 ttggcaatgg tttcacggtt ttcgagcttg agtaaaactt cttcgccttt ttcaatcgta
     9121 atcgatccat tgggaaaacg cccgacgcgt agtttcggac cctgcaaatc ttgtaatatg
     9181 gctaccggtt ttcctagagc agtaga
//
LOCUS       DAOMXO010000010         8829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_4190_length_8829_cov_4.656371, whole genome shotgun sequence.
ACCESSION   DAOMXO010000010 DAOMXO010000000
VERSION     DAOMXO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4190_length_8829_cov_4.656371"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PLH57_00460"
     CDS             <1..1047
                     /locus_tag="PLH57_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRK01108.1"
                     /translation="QYNLRTLNRRTILFVDEIHRFNKNQQDALLPFVEDGTVTLIGAT
                     TENPSFELNSALLSRAPIFVLKTLEFEALIEILKRAATDPVEGLGLKSQQLPESLFRI
                     IAQRANGDARRALGILEDFHVYALSELSDASPLTNERASELLEKSVGQKFIRYDARGE
                     EHYNTISAFIKSLRDSDPDAALYYLARMLEAGEEPLFIARRMVIFASEDIGNAEPRGL
                     QVALAVKEAVDFVGMPEARINLAHGVTFLAACPKSNASYVGIESALEDVRNHGNLEIP
                     LHLRNAPTRFMKDQGYGKNYQYAHDFEDGITEQVNLPEQLNSRRYYQPKPVGLEKSIS
                     DRLLALRTRKKPQN"
     gene            1120..1719
                     /locus_tag="PLH57_00465"
     CDS             1120..1719
                     /locus_tag="PLH57_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01109.1"
                     /translation="MQSLTIQRAVFILLILFSFFSPTYAKAADFEIFDGRVYNGKFYP
                     RIDIIEWGTPSHMEFHIYSKGKPIELGFELEDKTGRKVMLVNYHITERGERLCRRVLA
                     PAHFHSGFMVYVDKTDPDFDNVIVSVHELPAKRGLVKLIRPHSYAACELDKPDRSVAS
                     TGEGEYKNIAPPHVDQSKTKVDGSAIKKPGQAVPFGDIF"
     gene            complement(1746..3065)
                     /locus_tag="PLH57_00470"
     CDS             complement(1746..3065)
                     /locus_tag="PLH57_00470"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRK01110.1"
                     /translation="MKSLTDMQDLVALCKRRGVVFQSSEIYGGINACWDYGPLGVEMK
                     NNVKQLWWRSMVDRDDVEGLDAAILMHPRVWEASGHVAGFSDPLVDCKKCKQRFREDK
                     IPDENRKSKKCPNCSGELTEPRMFNLMFKTFMGPVEEDAAVVYLRPETAQGIYVNFLN
                     VASSSRQKIPFGIAQIGKAFRNEITPGNFIFRTREFEQMEMQFFVKPGEDEKWFEYWK
                     EQRLKWHLANGIRKEKLRFHEHRDGELAHYAKKAYDIEYEFPFGWQECEGIHNRTDFD
                     LGRHQEYSKKKQEYYDEPTKERYIPYIVETSAGCDRTLLVTLADAFRQEEDRVFLALH
                     PNVSAYKVAVFPLMKKPELMSVAEKVYDEIRKKYRATFDESGSIGKRYYRQDEIGTPF
                     CVTIDFDTVEKGVLTVRNRDTKVQEKLDASKVLEYLTDQISSWKLTV"
     gene            complement(3075..4790)
                     /locus_tag="PLH57_00475"
     CDS             complement(3075..4790)
                     /locus_tag="PLH57_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRK01111.1"
                     /translation="MKSFLRLVPFIKPQWRLLLVAGLLTIPLSAVRFSPAPLLKFLQD
                     DVLAQRSYDKLLYLAFAVVGIYCVNVIVRFSSTFLARLANERIMRDIREKLFNHYLGL
                     SSSFFNESSIGQLISRVTNDVFYVSQGTINLIALVRDSFTFIGLLIYAIHLNVKLLAL
                     MLVIAPMLTWLGKRSGKLMKGYALKMQEANGNVYSALQEAFSGFRVIKGFGLERLTFD
                     RFKKRNDEYVNFALKAARVEEIGGPSVEFMGAIAAALIIYVGGRDVLQDRLTTGELLA
                     FFTCFGLMINPIRNLNDINMKLNLAGASAERIHETLALRSAIVEKPNAKVLSGREHAI
                     AFENVSFRYADDLPFVFRQVSFEVPKGKMFAVVGASGQGKSTLVNLLPRFYDPSDGRV
                     LIDGSNVSDFTLSSLRKQIAVVTQDVFLFNDSVRANIAAGRTGATEEEIVSAAKSAQA
                     WDFISRLPEGLDTVVGDRGQKLSGGERQRISIARALLKDAPILILDEATSSLDSESEK
                     AVQAALEKLMVGRTTLVIAHRLSTVRHADQILVLSGGKVVEIGTHDQLMSQRGEYAKF
                     YSLLV"
     gene            4829..5836
                     /gene="galE"
                     /locus_tag="PLH57_00480"
     CDS             4829..5836
                     /gene="galE"
                     /locus_tag="PLH57_00480"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRK01112.1"
                     /translation="MSRTQKRIQNILVVGGAGYVGSHVAKCLEKAGYNPVILDDLSTG
                     HRKAARSATFYQGHYGDRELLKSIFSKHSISAVMHFGAKALVGESVADPLLYASSNIA
                     GTIALLEGMRAANVNRFIFSSTCNVFGDPKEVPIHEGVQKLPVNPYGFSKFAVEQVLQ
                     HMDQAYQFRSASLRYFNAAGADPEGELGEDHRCETHLIPNAIRSLTGRSPALTIFGND
                     YPTPDGTCIRDYVHVNDLASAHISALELIDSQNRSVDFNLGSEQGYSVNEVIHMLEKV
                     SGKQVPRKLGARRPGDAPRLVADSSKAKRELLWKTSFDLEAIVRTAYQWLAKNPEGYA
                     S"
     gene            5833..6417
                     /locus_tag="PLH57_00485"
     CDS             5833..6417
                     /locus_tag="PLH57_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRK01113.1"
                     /translation="MRQPALFLDRDGTINVDLIGNYVLHPDKLQLVPGAATALSEARA
                     AGFKLGVVTNQACIGKGLLTHEGLAKIHEHLEYLVRQEANLDRFHFDDLRYCPHRADE
                     GCACRKPNTGMLEAAIATLNPVLDRSFFIGDKPADLGCANKAGIRSILVLTGYGSETL
                     KEVEAGKCPTPYAVCTNLLEAVRWISEDLRTHQS"
     gene            complement(6367..7164)
                     /gene="recO"
                     /locus_tag="PLH57_00490"
     CDS             complement(6367..7164)
                     /gene="recO"
                     /locus_tag="PLH57_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRK01114.1"
                     /translation="MEHKSELGIILKTFPYQERDRVCVVLTESSGKITALAKGAIHSR
                     RFGGALDTLTCSRLRFIQKPNTEMGRIDEAVVHHEFTRLRTDYDRLALASFAAEFCLR
                     LVEPHAPARDLFVLLSNFLFHLDSDADGFYCTNVFLVKALHVMGYAPNLTSCSRCAVS
                     AHSCAEGDFYWDSDAGGIICASCAPHASKKQLYLGLDAHVLFSECLAKPFKVLVEAKS
                     LSQQTQQNLFAVLTDFLHHQVPGLPAGGFKSLRLVGAQVLGYPTNSL"
     gene            complement(7198..7839)
                     /locus_tag="PLH57_00495"
     CDS             complement(7198..7839)
                     /locus_tag="PLH57_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01115.1"
                     /translation="MKGIVFALFAVSVTLVCSGCPATDEALPNVPEIRYDVIEFSPRF
                     DFESPIPENGKIDIRYSDKSDDPYRNDTWTQDSDAIIGHFGFYLKNHSDSDIERVLVY
                     VHTEDPSKIIFPVFIEFIDQLMYSNSFVGIGPPDLSEGEYLYSNVIYPGRFARLHLGT
                     LKSNSQIDLEALIAGKPFMKFHIAARGWLKSSEEWVATHSPTEISFFTGDLIR"
     gene            7894..>8829
                     /locus_tag="PLH57_00500"
     CDS             7894..>8829
                     /locus_tag="PLH57_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01116.1"
                     /translation="MAKKRPMSLTERSILMQSSLLRRNWPKLIPANVKKALHLGGQIY
                     LVGTGSSFHVAQWASFVFSNAQAQSSWDTLYRAPNQRLSQDALYIVCSHRGAQGITRA
                     LLKHLRKHRLNAVLLCAEGSPTLEFPTVFTSPREQSQAHTMSVTAAIAALLTMADIQR
                     HKKPASVLKRLSLVAQKFDEGAFRCKMPFRFDANLPVTVIGGGELHAIAQELALKIRE
                     MCFLPAFAIALEEYLHGPYVATEPGETILFLAGGQGPRAVQALKTAKTLKANIISPSP
                     AILRLPPIEKSLGLLFWGQSLALELSRRLDTNPDLN"
BASE COUNT         2408 a         2257 c         1953 g         2211 t
ORIGIN      
        1 caatacaatt tacgcactct aaatcgccgc acgattttat ttgtcgacga gatccatcgt
       61 ttcaacaaga accagcaaga tgccctgctt ccgtttgtcg aggacggtac tgtgacgctc
      121 attggcgcga ccactgaaaa cccgagtttc gaacttaatt ccgcgttgct ttctcgagcg
      181 ccaatctttg ttttaaaaac gctcgaattt gaagctttga tcgaaatctt aaaaagggcc
      241 gccacggacc ctgtcgaagg tctggggcta aaaagtcagc aactcccgga atcacttttt
      301 cgaattatcg cccagcgcgc aaatggagac gctcgacgtg cgctcggaat tctcgaagac
      361 tttcacgtct acgcactaag tgaactcagt gatgcatccc cactcactaa tgaacgtgct
      421 tccgagcttt tggaaaaatc agtgggtcag aaatttattc ggtatgacgc gcgaggcgaa
      481 gaacactaca ataccatctc tgctttcatt aaatctctgc gagacagtga cccagatgct
      541 gcgctctatt atttggcgcg aatgctcgaa gcgggcgaag agccactctt catagctcga
      601 cgcatggtta ttttcgcatc agaagatata ggaaacgctg aacctcgcgg gttgcaagtt
      661 gcgctagctg ttaaagaggc cgtcgatttc gtcggaatgc ccgaagcccg catcaacctc
      721 gcgcacggag ttacgtttct cgctgcctgt ccaaaaagta acgcgtctta tgtcggcatc
      781 gaatccgccc tggaagacgt acgaaatcac gggaatttgg aaatcccgct tcacctacgg
      841 aacgctccca cgcgctttat gaaggatcag ggatacggaa aaaactatca atatgctcat
      901 gacttcgaag acggaattac cgagcaggtc aatttgccgg aacaattaaa ttctcgacgc
      961 tactaccaac ccaagcccgt agggcttgaa aaatcgattt ctgaccgcct tttagccctg
     1021 agaacccgta aaaaacccca aaattaaccc tctaatacct gataaaaaca ctaaagattt
     1081 aacccaaaac taccgactct acagtagggg agcgcgtcga tgcagagctt aactattcag
     1141 cgggcggttt ttatcctatt gatattattt tcttttttta gtccaaccta cgccaaagca
     1201 gccgacttcg aaatcttcga cggacgagtg tacaacggga agttctaccc tagaatcgac
     1261 atcattgaat ggggcacccc ttcccacatg gaatttcata tctattccaa aggaaaaccc
     1321 atcgaactgg gttttgaact cgaagataaa acaggacgca aagtaatgtt ggtaaactac
     1381 cacatcactg aacgaggaga gcggctgtgt cgtagagtat tggcgcccgc tcattttcat
     1441 tcgggcttta tggtctacgt agataaaaca gatccggact tcgacaacgt aatcgtgtcg
     1501 gtacacgagc ttccggctaa aagaggactc gttaaactta ttcgcccgca cagttatgct
     1561 gcttgtgaat tagataaacc ggacagatcc gtggcttcta ccggcgaagg cgaatacaaa
     1621 aacatcgctc cccctcacgt tgaccagtcc aagacaaagg tcgacggatc cgcgatcaaa
     1681 aaacccggtc aggccgtgcc atttggcgat attttttaaa tctaactttt attcaaattg
     1741 ccgatctaga cagttagctt ccagctcgaa atttgatccg tcaaatactc caggactttc
     1801 gaggcatcta acttttcttg caccttcgtg tcgcgatttc ttacagtaag aacacctttt
     1861 tcgaccgtat cgaaatcaat ggtcacacaa aatggcgtac cgatttcatc ttgacgatag
     1921 tagcgcttcc caatgctgcc cgactcatca aacgtagcac gatatttttt ccgaatttcg
     1981 tcataaacct tttcggccac tgacatgagt tctggttttt tcatgagcgg aaacacggct
     2041 actttataag ccgaaacgtt cgggtggagc gctaaaaaca ctcgatcttc ttcttgtcga
     2101 aaagcatcgg ctaacgttac cagcaaggtg cgatcacaac ctgccgaagt ctctacgata
     2161 taaggtatgt aacgttcttt ggtgggttcg tcgtagtatt cttgcttctt cttggaatac
     2221 tcttgatggc gacctaaatc aaaatccgta cgattatgaa taccttcgca ctcctgccaa
     2281 ccgaaaggga attcatactc aatgtcataa gctttttttg cgtagtgcgc caattctccg
     2341 tcgcggtgtt catgaaatcg cagtttctct tttcgaatac cgttggcaag gtgccattta
     2401 agtcgttgtt ctttccaata ttcaaaccat ttttcatctt cgccaggttt cacaaagaac
     2461 tgcatttcca tttgttcgaa ttctcgcgta cgaaaaataa agttccctgg tgtaatttca
     2521 ttacggaaag ccttaccgat ttgagcaata ccgaatggaa ttttttgacg tgaacttgaa
     2581 gccacattca aaaaattgac gtatattccc tgagccgttt ccgggcgcaa ataaacgact
     2641 gccgcatctt cttcgaccgg ccccataaat gttttgaaca tcaagttaaa catgcggggc
     2701 tcggtaagct ccccgctaca gtttggacat ttttttgatt ttcgattttc gtctggaatt
     2761 ttatcttcgc gaaagcgttg tttacatttt ttgcagtcaa ccaaggggtc gctgaagccc
     2821 gctacgtggc cactcgcttc ccagactcgc ggatgcatga gaatcgccgc gtcgagtcct
     2881 tcgacatcgt cgcgatctac catagaacgc caccaaagct gctttacgtt atttttcatt
     2941 tctacgccaa ggggaccgta atcccaacac gcgttgatac cgccatagat ttcagaactc
     3001 tgaaacacga cgccacgccg cttacaaagc gcaactagat cctgcatgtc tgttaaactt
     3061 ttcattcgag ctcctcaaac caaaagagag taaaatttgg cgtattcgcc gcgctgagac
     3121 attaattgat catgtgtgcc gatttccact acttttccgc ccgacaaaac aagtatttgg
     3181 tccgcatgcc gaacagtgga gagtcgatgg gctattacta gcgtggttcg gcctaccata
     3241 agcttctcca acgccgcctg tactgctttt tcggactcag agtcgaggct agaggtagct
     3301 tcatccagaa tcaatatcgg tgcgtctttt aacaatgcgc gagcgattga aatgcgttgc
     3361 ctttccccac cagagagctt ctgtcctcgg tcccccacta cggtatctaa cccctctgga
     3421 aggcggctaa taaaatccca agcttgcgca gatttagccg ccgaaacgat ttcttcttcg
     3481 gtggcgccag ttcggccggc agctatattg gcccgaaccg aatcgttgaa taaaaataca
     3541 tcctgagtca ctacagcgat ctgttttcta agactactca atgtaaaatc gctcacattc
     3601 gacccgtcaa tcaacacgcg gccatccgat gggtcataaa aacggggaag taaattaaca
     3661 agggtcgatt tgccctgccc actggcccca acgacggcaa acatcttgcc cttgggtact
     3721 tcaaacgaca cctgccgaaa tacgaacgga aggtcgtccg cgtagcgaaa gcttacgttt
     3781 tcgaaggcga tcgcatgttc gcgaccggaa agtaccttag catttggttt ttcaacaatc
     3841 gcactacgca aagctagcgt ttcgtgaata cgttcagctg atgcgcccgc gagattcaac
     3901 ttcatgttga tatcgttcaa gttacgaatg ggattaatca ttagaccaaa acaggtaaaa
     3961 aacgccaata attcgccggt agtcagcctg tcttgcagca catctcgacc gccaacgtag
     4021 ataatcaaag ccgcagcgat ggcccccatg aactctaccg aaggtccacc tatttcttcg
     4081 accctggctg ccttcaaagc aaagtttacg tattcgtcgt ttcgtttctt aaatcgatcg
     4141 aaggttaatc gctcgagccc aaaccccttg atcaccctaa accccgaaaa cgcttcctgg
     4201 agagccgaat acacgttgcc attagcctct tgcattttaa gagcgtaacc cttcatgagt
     4261 ttgccagagc gcttgccgag ccaggtgagc attggggcta ttaccagcat gagcgccagt
     4321 aacttgacgt taagatgaat ggcgtaaatt agaagcccga taaaggtaaa actgtcacgc
     4381 accaacgcaa ttaaattgat agttccctgg ctgacataaa acacgtcatt ggtaacacgc
     4441 gaaatcagtt ggccaatgga actttcatta aaaaaggaac ttgatagtcc caagtaatga
     4501 ttaaagagtt tttcgcgaat gtcgcgcata atacgttcgt tggcgagtcg cgccaaaaaa
     4561 gtgctcgaaa atcgcacaat cacattcaca caatagattc ccacgacagc aaacgctaaa
     4621 tacaagagct tatcgtagct tctctgagca agcacatcgt cttgtaagaa tttcaaaagt
     4681 ggcgctggac taaaacgcac cgcagaaagg ggaattgtca acaaaccggc cactagcaaa
     4741 agccgccact gcggtttaat gaaaggaacc aagcgaagaa aagatttcat ttcattttca
     4801 tctaccccaa gctaaaaaag aagtcactgt gtctcgaacg caaaaaagaa tccaaaacat
     4861 tctagtcgtg ggtggcgccg gatatgtggg cagccacgtt gccaaatgcc tggaaaaagc
     4921 aggatacaat cccgttattt tagatgatct gagtacaggc catcgaaagg cagccagatc
     4981 tgctactttt taccaaggcc actacggcga tcgggaactg ctaaaaagta tcttttctaa
     5041 acattcgatc tctgcagtta tgcattttgg agcaaaggct ttggtcggag aatcagtagc
     5101 ggaccctcta ctttatgctt cttcgaatat tgcagggacc atcgccctac tcgaaggcat
     5161 gcgtgcggcc aacgtaaatc ggttcatatt ttcgtctacc tgcaacgtat ttggggatcc
     5221 aaaagaagtt cctatccatg aaggagttca aaaactcccg gtcaacccct acggttttag
     5281 caagttcgca gtcgagcaag tactacagca catggaccag gcttatcagt tccgatcggc
     5341 aagtctgcga tactttaatg ccgccggcgc cgatccggaa ggcgagctag gtgaagatca
     5401 tcgatgtgaa acacatctta ttccgaacgc gattcgttca ctcaccggtc gctcaccggc
     5461 gctaaccatt tttggaaacg actacccgac gcccgatggc acttgtattc gggattatgt
     5521 tcacgtgaat gatttagctt cagcccacat cagtgccctc gaattaattg actcccaaaa
     5581 tcggagtgtt gatttcaacc ttggtagcga gcaagggtat tcagtcaacg aagttatcca
     5641 catgttggaa aaagtgtcgg gaaaacaagt tccccgaaaa ttgggagcta gacgtcccgg
     5701 cgacgcccct agactggtag cagattcttc gaaagccaaa cgcgaactct tgtggaaaac
     5761 tagctttgat cttgaagcaa tagtacgcac cgcctaccag tggcttgcca aaaacccgga
     5821 aggttacgct tcgtgaggca acctgcgcta tttctagatc gcgacggaac tatcaacgtc
     5881 gatctcatag gaaactacgt attgcacccg gacaagctcc aactggtacc aggcgccgca
     5941 actgcgctct cagaagcgcg agcggctggc tttaaattgg gagttgtcac caaccaagcc
     6001 tgcatcggga agggtttact gacccacgag ggattggcta aaattcacga gcatcttgaa
     6061 tatcttgtcc gtcaggaagc gaaccttgat cgcttccact tcgacgatct tcgctattgc
     6121 ccccaccgag ccgacgaagg ttgcgcctgc cgaaaaccca atacgggaat gctcgaggcc
     6181 gcaatcgcta ctctaaatcc cgttcttgat cgttcatttt tcatcggtga caaacccgcc
     6241 gatcttggtt gcgcaaataa ggcaggaata cgttcaattt tggtattaac gggttatggt
     6301 tctgaaacgc tcaaagaagt tgaagctggg aaatgcccta ccccctacgc cgtctgcacg
     6361 aatcttctag aggctgttcg ttggatatcc gaggacctgc gcacccacca atcgtaggct
     6421 cttaaatccc cccgcgggca accctggcac ttgatggtgc aaaaaatctg tcaatacggc
     6481 gaacaaattc tgttgagttt gctgtgacag agatttcgct tcgaccaaaa ccttaaacgg
     6541 tttagccaaa cactccgaaa ataatacgtg ggcatctaac cccaagtaga gttgtttctt
     6601 agaagcatga ggcgcacacg atgcgcaaat tatcccaccg gcgtcgctat cccaataaaa
     6661 atcgccttcc gcacaagagt gcgccgaaac tgcgcagcgc gaacaactag taagattggg
     6721 ggcgtatccc attacatgta gcgctttcac taaaaagaca ttggtacaat aaaaaccatc
     6781 tgcatcggag tccaagtgaa acaggaaatt gctcaagagc acaaatagat cacgagcggg
     6841 agcgtgaggc tccactagcc gcaagcaaaa ttcggcggca aaactagcga gagcgagccg
     6901 atcatagtcc gtgcgtagcc tggtgaactc gtgatgtacc acagcttcgt cgatacgacc
     6961 catttcagtg ttgggttttt gaataaatct caatcgtgaa caagtaaggg tatcgagcgc
     7021 tccgccgaaa cgacgcgaat gaatggctcc tttcgctaaa gcggtgatct tgccgctcga
     7081 ttcagtaagg actacacaaa cgcgatcgcg ttcctgatat ggaaaggttt ttaatattat
     7141 tccgagttcg gatttatgtt ccatagcgcc ttaaggcaaa aaaacgtcag cctggaatca
     7201 ccttatcaga tcacccgtaa aaaaactgat ttcggtagga ctatgagtcg caacccactc
     7261 ctcagagctt ttaagccacc ctcgcgcggc aatatgaaac ttcataaacg gcttaccagc
     7321 gataagtgcc tcaagatcta tttgggaatt tgatttgagc gtaccgaggt gcaacctcgc
     7381 aaatcgcccg ggataaatta cattggaata taggtactca ccttccgata aatcgggcgg
     7441 tccgattccg acaaaactgt tggaatacat aagttgatct atgaattcga taaacaccgg
     7501 gaaaattatt ttcgatgggt cttccgtatg aacataaacc aacactctct ctatgtcgga
     7561 gtcgctatga ttttttaggt aaaaaccaaa gtggccaata atggcgtccg agtcttgagt
     7621 ccaggtgtca ttgcgatagg gatcatcact cttatcggaa taccgaatgt cgattttgcc
     7681 attttccgga attggagact cgaaatcaaa gcgcggcgaa aactcgatca catcgtagcg
     7741 aatttcgggt acattcggaa gcgcttcgtc ggtcgcggga caaccgctac aaacaagcgt
     7801 caccgaaacg gcgaacagag caaaaactat ccccttcatc gagcccccta ttgaaagttg
     7861 cgcctactgc cgctactaat caaagtcaac tttatggcca aaaaacgtcc aatgtccctc
     7921 accgaacggt ctattttaat gcagtcttcg cttttgcgaa gaaactggcc gaaactcatt
     7981 ccggcaaatg taaaaaaggc gcttcatctt ggtggccaga tctaccttgt cggcactggc
     8041 agcagctttc atgtcgcaca gtgggcgagt ttcgtgtttt caaacgctca ggcgcagtct
     8101 agctgggaca ctctttatag agctccgaac caacgattgt cccaagacgc tctctacatt
     8161 gtttgttctc atcgtggggc tcaaggtatt acgcgggctc tattgaaaca tctgaggaaa
     8221 catcgtctta acgctgttct actctgcgca gaaggctcac caacgctaga gtttcctacc
     8281 gtctttactt ctccacgaga acagtcccag gctcacacaa tgagcgtgac ggctgcgata
     8341 gcggcacttt tgaccatggc agacattcaa cgtcataaaa aaccagcttc tgtcctaaaa
     8401 agattgtcgc tggtggcgca gaaattcgac gaaggcgcct ttcgctgcaa aatgcctttt
     8461 cgcttcgatg ccaatttgcc agttacggtt attgggggcg gagaacttca cgcgattgcc
     8521 caggagctcg cacttaaaat tcgagaaatg tgttttctgc ccgccttcgc aattgcttta
     8581 gaggagtatt tgcacggtcc atacgttgcg accgaaccgg gggagacaat tttgttttta
     8641 gccggcggcc aaggtcccag ggcggtacag gcgctaaaaa ccgctaagac gctgaaagct
     8701 aacattattt cgccttctcc tgcgattttg cgacttccac ccattgaaaa atcactcggc
     8761 ctgctttttt gggggcaatc gttagcactt gagctttccc gccgacttga tacaaatccg
     8821 gatctcaat
//
LOCUS       DAOMXO010000011         8687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_4271_length_8687_cov_5.386469, whole genome shotgun sequence.
ACCESSION   DAOMXO010000011 DAOMXO010000000
VERSION     DAOMXO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4271_length_8687_cov_5.386469"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLH57_00505"
     CDS             <1..304
                     /locus_tag="PLH57_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRK01117.1"
                     /translation="VILIMGGRDKDQDFTPLMELVKKRVKNLILVGEAKEKINRILGD
                     FSETFLVGTFEEAVLIGFQKSRNGDIVLLAPGCASYDMFRNYEERGDYFKKMVSQL"
     gene            324..1433
                     /gene="ftsW"
                     /locus_tag="PLH57_00510"
     CDS             324..1433
                     /gene="ftsW"
                     /locus_tag="PLH57_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRK01118.1"
                     /translation="MRSRSFLARHSEHAFWIAVFALVATGLVMVYSSSFILAAERFKD
                     GLYFFKRHVLYCGFGTLFFILGRHLNSYQLQKLAKPLLIFATLALVATLIPGIAHRVG
                     GASRWISFFGFRFQPGELAKFAVILFVASQLQKKLAYQDSWKTGFLTYFVGVIPIYFA
                     LLLQPDFGTFVLLIATTMCMLWGAGVRLKYLLGTTLAFFPIAATLILAKPYRKARLLA
                     YLDPWSDPAHKGFQIIQSFVAFYEGSFFGVGLGNSREKLFYLPEAHNDFIFSVIGEEL
                     GLLGVVFVIALFLLIIRHGIQTALRTQNTFNRGLALGITSVLGLQAFCNMGVVTGLLP
                     TKGFPLPLVSYGGSSLLVTLFMLGILSRLAETKSQ"
     gene            1430..2506
                     /gene="murG"
                     /locus_tag="PLH57_00515"
     CDS             1430..2506
                     /gene="murG"
                     /locus_tag="PLH57_00515"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRK01119.1"
                     /translation="MKLVIAGGGTGGHIFAGVAIAQEFKSQSAANEVLFVGSEYGLEK
                     RLVPKWGFPLHTIKIGKLVGQGLLRRLWTLLQIPISVWSCIRVLRSYRADMVIGVGGF
                     AAGPCIVAARILGLPIGVLEQNSVMGFTNRVSARLAHHIFLAFDEIPQGIPRHKCVVT
                     GNPTRSQLHSKEKTGDHFTIFAFGGSQGATGINRLLTDAAKKLAEEKHRIAIIHQTGE
                     KDFEWVVEAYKKIEYPAQVFKFIDDMQKMYDQASIVICRAGSGTISELGATKNAAIFI
                     PFPYAAGNHQEHNARNVQAAKAGQVLLQTESNGDQLAEMILALRKNPEQLAVMRQNIT
                     NFFRPDAPKRIVDVMKTRVLTQNV"
     gene            2499..3917
                     /gene="murC"
                     /locus_tag="PLH57_00520"
     CDS             2499..3917
                     /gene="murC"
                     /locus_tag="PLH57_00520"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRK01120.1"
                     /translation="MYKRIKEHIHFVGIGGIGMSGIAEILVRMGFPVSGSDLSDSDTV
                     RRLRDLGVRVEIGHRAENIEGAKAIVTSSAVTKTNPEVLEAHRRHLPIVARAEMLAEL
                     MRLKYGLAVAGTHGKTTTTSLLGSILTYAELDPTIIVGGKVDALGGNAKFGQGQFLVA
                     EADESDGSFLKLSPVVTIITNIDNDHLDHHGSMQLLRTAFAEFANGVPYYGRNIVCID
                     DAEVQDLIKSIEKPVWTYGFSKAADFQIRDFEHAGINGSRFSVWRSGQFLTKVTLNIP
                     GEHNARNAVGALAAAIEVDVSVEIAVRALASFTGVRRRFETKARFEKQNITIVDDYGH
                     HPTEIDATLAAAYLSQKANGHQRVVVIFQPHRYSRTASCWNQFAASLSKAAQVFLLDV
                     YAAGEAPITGVNSATLTEALKTSGVPVEYLPVPEQTSAAEGLAVVAKTIKSRIKNGDL
                     IITLGAGNITTLGPLLASELSK"
     gene            3914..4825
                     /gene="murB"
                     /locus_tag="PLH57_00525"
     CDS             3914..4825
                     /gene="murB"
                     /locus_tag="PLH57_00525"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRK01121.1"
                     /translation="MSLPVCVKENVSLKKHCYYRIGGNALYFATPRNIDELPELVAWI
                     KKNQLRYYILGAGSNTLFDDLGFNGVVINTSELDTRLTLEGETVDAGCSVTNIQLLRL
                     CMREGLRGLDFLVGIPGNVGGAVFMNAGTQKGEVVDSVFEVSAYNLANEKIRSYSTNK
                     KSFIYRSQTFLAPHEIIIRSKFRISRDQPERVQNDIQDLLLKRKKSQPIDKPSCGSVF
                     QNPSATQSAWKLISDAGLRGRRAGDAEISTMHCNFIVNNGNAKSADVRSLIELAKKEV
                     KAQFGILLKEEVKIIPQSGGILLGDHK"
     gene            4809..5597
                     /locus_tag="PLH57_00530"
     CDS             4809..5597
                     /locus_tag="PLH57_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK01122.1"
                     /translation="MATTNEKKALITGASEGIGRDLCVLLAKQGFTITGVARNSSRLE
                     SLISELKKISPAAHDFQVCDLGQPSDVARLGAFIESHHYDVLVNNAGFGAYGEFSRTP
                     LQKLESMIQLNCTALVALAHAFLRGARPGDALVNIASVAAFISLPGNAVYSATKAFVH
                     NLTVSLWFEGKRRGVYVCSICPGYTDTQFASRAGGDPNKAPRTFAITSEAVAASIVRA
                     IQKRTSVQVVPDVMNRIMVFLLRFLPQTWIVRAVHRATRKAFGE"
     gene            complement(5609..6217)
                     /locus_tag="PLH57_00535"
     CDS             complement(5609..6217)
                     /locus_tag="PLH57_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01123.1"
                     /translation="MTKLFRRWLGDLKENFSANKGGVLSGTRSWERDFSGDIHSNDAQ
                     YGVFLGALSLIPALATAYLWSHSLPPPPRVHVMAHLFGAPLQFVITGFLCSVLFFLGS
                     HVNRAPKIYPVAFKLMLRVMAVYPILATMFYFRFGGVFVLLLFGVFVIRGALRTYRIP
                     KANVIIFFGIAYVTFALLQLQNAISPPLDRPQKKLIQQNENR"
     gene            6288..8108
                     /locus_tag="PLH57_00540"
     CDS             6288..8108
                     /locus_tag="PLH57_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRK01124.1"
                     /translation="MIKNLKRRLLGSPIASALAHSERINKRRALAVFSSDALSSVAYA
                     TQEILLVLVLAGGAALSYSIPISGAIICLLFLVVVSYRQTIREYPNGGGAFSVTKENL
                     GPSWGLVAGAALLIDYVLTVAVSVAAGVAALTSAFPSLFQYKITICAVAIGVLTIANL
                     RGVRESATIFAFPTYGFIVGILSMLAIGTYKLYTGEFTHIDPPATEGLEPLTVFLLLH
                     AFSSGCTAMTGVEAVANGVPVFQPPESKNASKTLIIMACLLTTMFFGITYLSHYHGIA
                     PNEQETVLSQLARSIYGSTWPYYSIQILTMGILFLAANTSYAAFPRLGSLLAKDRYLP
                     RQFASLGDRLVFSNGILILGVVSTGLIYLFDADTHALIPLYAVGVFLSFTLSQAGMLR
                     HWERSRARGWIRHAIVSGIGMTVTAVVTTIILTTKFTDGAWLITIAVPALIILFRQVH
                     HHYLSVGKQLSLVGVDPKSLPHELKHTVLLPISGVHRGVIEAMRYAQSIATDVRAVYV
                     EIDKESTERFQQDWQRWGRGVPLVVLRSPYRSVVTPLLRYIDEVEDSNQDDMITVIIP
                     EFVTAKWWQQFLHNQTALIIRAALLFKRNKVVTSVRYHLD"
     gene            complement(8117..>8687)
                     /locus_tag="PLH57_00545"
     CDS             complement(8117..>8687)
                     /locus_tag="PLH57_00545"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRK01125.1"
                     /translation="NRIARGFWKNCGSFSSLKNMFLFGHTGLAVGTAHWINKKFKKKI
                     DLRYVALMSMSADLIDKPLGLSSPEFFRFNTRLIGHNLFAIVFAVSLLLAFSRRRSAI
                     MLYALFYCSHLLLDQMWIKSPDIFYFPFGGPVQWHEPDLVRRWTDVLKTPYTLGGEVL
                     GFSILCYLLGKYQLYRAKNYSNFIKTGTL"
BASE COUNT         2279 a         2104 c         2006 g         2298 t
ORIGIN      
        1 ggttatattg attatgggcg gccgagataa agaccaggat ttcacacccc tcatggaact
       61 agtaaaaaaa cgggttaaga acctgattct ggtgggcgaa gccaaagaaa aaatcaaccg
      121 catcctaggc gatttcagtg aaacctttct tgttggtacc tttgaagaag ctgtattgat
      181 cggttttcag aagtcccgaa acggggacat cgttttgctc gctcctggtt gcgccagtta
      241 cgacatgttc cgtaactacg aagagcgggg cgactacttc aaaaagatgg tttcgcagct
      301 ctaaaaagct tagactttag tggatgagaa gccgttcgtt tcttgcccgc cattcggagc
      361 atgcattttg gatcgccgtg ttcgcgcttg tcgcgacagg gttggtcatg gtttacagtt
      421 cgagttttat tctcgctgct gaacgtttca aagacggact ctattttttt aaacgccatg
      481 ttctctactg cggatttgga actctgtttt tcatactggg ccgacactta aacagttacc
      541 agctgcagaa acttgcaaag ccattgttaa tctttgccac gcttgcatta gtggccacgc
      601 ttattccagg tatcgctcac cgagtcggag gcgcttcccg ttggatttct ttttttggat
      661 ttcgttttca accgggcgaa ttggctaagt ttgctgtcat tttattcgta gcttcgcagt
      721 tgcaaaaaaa attggcctat caggacagtt ggaaaacggg ttttctaacc tacttcgtcg
      781 gtgtgattcc catttacttt gcgctactcc tccagcctga ctttggcacc ttcgtacttc
      841 taatcgccac gaccatgtgc atgctttggg gggctggtgt gcgcctcaaa tatttactcg
      901 gcaccaccct ggcctttttc ccaatagcgg cgacgctcat actcgcaaaa ccatatcgga
      961 aagctcgttt acttgcttat ctagaccctt ggtccgatcc cgctcacaaa ggattccaaa
     1021 ttattcagag tttcgtcgct ttttatgaag gcagtttttt cggagtgggg ttaggcaatt
     1081 cccgtgaaaa acttttctat ctaccggaag ctcataacga ttttattttt tcagttatcg
     1141 gcgaagaact cggactactc ggagtagtat ttgttatcgc gctattttta ttaattatac
     1201 gccacggaat tcaaaccgca ctacgtactc aaaatacctt taaccgagga ctcgcgctcg
     1261 ggataacaag cgttttgggt ttgcaggcgt tttgcaatat gggagtagtc accggattgc
     1321 ttccgaccaa gggattccct ctgccgcttg tgtcctacgg tggtagttca cttctggtaa
     1381 cattattcat gctgggaata ctctctcgcc tagcggaaac gaagtcccaa tgaaactcgt
     1441 tattgccggc ggcggaaccg gtggacacat tttcgctggc gtcgcaattg cccaggaatt
     1501 taaaagccag agtgccgcaa acgaggttct atttgtcggt tctgaatacg gactcgaaaa
     1561 aagactggta cccaaatggg ggtttccact acatacaatc aaaattggca agttagtcgg
     1621 ccagggtctt ttacgtagac tatggacgtt gctccaaatt cctatttccg tctggtcgtg
     1681 cattcgagtt ctaagatcct atcgagcaga tatggtaatc ggagtaggcg gcttcgccgc
     1741 agggccctgc atagtcgcgg ctcgtatact tggtcttcca attggcgtat tggagcaaaa
     1801 ctcggtaatg ggttttacca atcgcgtctc ggctcgcctt gcacatcaca tatttttagc
     1861 tttcgacgaa attccgcaag gcattcctcg tcacaaatgt gtcgtaactg gcaatcccac
     1921 tcgcagccag ctgcattcta aagaaaaaac tggtgatcac tttacgatct tcgcgtttgg
     1981 cgggtcccaa ggcgcgactg gtatcaatcg tctacttacc gacgcggcaa aaaaacttgc
     2041 cgaagaaaaa caccgaatcg cgatcattca ccaaacaggt gaaaaagatt tcgaatgggt
     2101 cgttgaggcc tacaaaaaaa ttgaatatcc ggctcaggtt tttaagttca tagacgacat
     2161 gcagaagatg tatgatcaag cttccattgt tatttgtcgc gctggtagtg gcacaatttc
     2221 ggagttaggt gccactaaaa atgccgcaat atttattcct tttccgtatg ctgccggtaa
     2281 ccaccaagag cataatgctc gtaacgttca agcggccaag gccggtcagg tgttgctaca
     2341 gaccgaaagt aatggcgacc aattagcaga aatgatactc gccttgcgaa agaaccctga
     2401 acagttggcc gttatgcgac aaaacattac caactttttt agacccgacg cccccaaaag
     2461 aattgtcgac gtcatgaaaa ctcgggtact tactcaaaat gtataagaga ataaaagaac
     2521 acattcattt cgtaggcatc ggtggaatcg gcatgagtgg aattgccgag attctcgtac
     2581 gcatgggttt tcccgtttcg ggttctgacc tatcagactc cgacaccgtt cgacgtctcc
     2641 gggatctcgg ggtgcgagta gagattggcc accgtgctga aaacattgaa ggcgccaagg
     2701 ctatcgtaac ctctagtgcc gtgactaaaa ctaatcccga agtgctcgaa gctcatcgcc
     2761 gccaccttcc aattgtggcg cgtgcggaaa tgctcgcaga acttatgcgc cttaaatacg
     2821 gtctcgcggt cgccggtacg cacggaaaga ccaccacaac cagtttactt ggcagcatac
     2881 ttacttacgc ggaactggac ccaacaatta tcgtaggtgg gaaagttgat gcattgggcg
     2941 gaaacgctaa atttggtcaa ggacagtttt tagtagctga agctgacgaa agtgacggct
     3001 cctttttaaa actctctcca gtcgttacga tcattaccaa tatcgacaat gatcatctcg
     3061 accatcatgg tagcatgcaa ttgctgcgca ccgcctttgc agagttcgca aatggtgtgc
     3121 cttattacgg acgcaatata gtttgtatcg atgacgcaga agtacaagac ttgatcaagt
     3181 ctattgaaaa accggtgtgg acctatggtt tctcgaaggc ggccgatttt caaattcggg
     3241 actttgaaca cgctggaatc aatggttctc gatttagtgt ttggcgttcc ggacaatttt
     3301 tgaccaaggt cacgctcaat attccgggtg aacacaacgc gcgaaatgcc gtaggcgctt
     3361 tagctgcagc catagaagta gacgtgtcag ttgaaatagc agtacgagca ctcgcttcat
     3421 ttacaggggt tcgccgcaga tttgaaacga aggcgcgctt tgaaaaacaa aacattacaa
     3481 ttgtcgacga ctatggccac catccaactg aaatcgacgc cacattggca gctgcctatt
     3541 taagtcagaa agccaacggg caccagcgcg ttgtagttat atttcagcct catcggtatt
     3601 cacgaaccgc ctcttgctgg aatcaattcg cagcgagcct ctctaaagcc gcgcaggtat
     3661 ttttactcga cgtatatgct gcaggcgaag cgcccatcac gggcgtaaac tcagccaccc
     3721 tgactgaggc actcaaaact tcgggagtac ccgtggaata tcttcctgtg ccggaacaga
     3781 ctagtgctgc cgagggctta gcagtggtcg cgaaaaccat aaaatctcga ataaaaaatg
     3841 gagacctaat cataactctg ggtgcgggca atatcacgac tctgggtccc ttgttggcta
     3901 gtgaactttc aaaatgagct tgccggtttg cgttaaagaa aatgtaagcc tgaaaaaaca
     3961 ttgttactat cgcatcggtg gtaacgcact ctacttcgcg actcctcgaa atattgacga
     4021 attacccgaa cttgttgcct ggataaaaaa aaaccaactg cggtattaca ttttgggggc
     4081 tggctcgaac actttgttcg acgatctagg gtttaacggt gtcgtaatta acacctcaga
     4141 gctcgacact cggctcacac tcgaaggtga gaccgtagac gccgggtgtt cagtgacgaa
     4201 tattcagttg ttgcgtttgt gcatgcgaga aggactgcgc ggtttagact ttttggttgg
     4261 aattccagga aacgtcggtg gtgccgtatt catgaacgcc ggcacacaaa aaggtgaagt
     4321 ggtcgattcg gtatttgaag ttagtgccta taatttagct aacgaaaaaa ttcgatccta
     4381 ttcgacgaat aaaaaatcat ttatctatcg gtcccaaacc ttcctcgcgc cccacgaaat
     4441 aattattcgc tcaaaatttc gcatttctcg ggaccaaccg gagcgtgtac aaaacgacat
     4501 tcaagaccta ctacttaaac gaaaaaaatc tcagccgatt gataagccaa gttgcggaag
     4561 tgtgtttcag aacccatctg ccactcaaag cgcctggaaa ttaatttcag acgccggcct
     4621 tcgcgggcgg cgtgcaggag atgccgaaat tagtacgatg cattgcaact ttattgtgaa
     4681 caatggcaat gccaaatctg cagacgtacg ttctttaatc gaacttgcaa aaaaagaagt
     4741 taaggctcaa tttggaattc tcttaaagga agaagttaaa attattccac aaagcggagg
     4801 aatactactt ggcgaccaca aatgaaaaaa aggctcttat caccggcgct agtgagggaa
     4861 taggccgaga tctctgcgta ttgttagcta aacaaggttt tacaattacg ggggtggcca
     4921 gaaactccag ccgactcgaa tcgctcatta gcgaattaaa aaaaatctcc ccagcggccc
     4981 atgattttca ggtctgcgat cttggacaac caagcgacgt tgcccgctta ggtgccttca
     5041 ttgagagcca ccactacgac gtgttggtta ataatgcggg tttcggagct tacggtgaat
     5101 tttcccgaac gccacttcaa aaactcgaga gtatgattca gctgaactgt accgccctgg
     5161 tagctttagc tcatgcattt ctgagaggcg cccgaccggg agacgcactc gtaaatatcg
     5221 caagcgtggc ggcctttata tccctgcccg gcaatgccgt ttattccgcg accaaagcct
     5281 ttgtgcacaa tcttacggtt tcgctctggt tcgaaggtaa acgaagagga gtctacgttt
     5341 gttctatttg cccaggttat accgataccc agtttgcctc acgggccggc ggtgatccca
     5401 acaaagcacc tcgaacgttt gcaataactt cagaggcagt ggcagctagc attgtccgcg
     5461 caattcagaa acgcaccagt gttcaggtgg tgccggacgt catgaatcga atcatggtgt
     5521 tcttattacg atttctgccc caaacttgga ttgttcgcgc cgtacaccga gcgactcgca
     5581 aagcctttgg cgaataaaat ttttaagctt aacgattttc gttttgctga attagttttt
     5641 tctgtggccg atcaagcggg ggactgatcg cgttctgaag ctgcaaaaga gcgaacgtta
     5701 cgtaagcgat cccaaaaaag atgatcacgt ttgctttggg aatccgatag gtacggagcg
     5761 cgccccgaat aacaaacacc ccgaacaaca gtaaaacaaa aaccccacca aacctaaagt
     5821 agaacatggt tgcaagaatg ggatacaccg ccatcactcg cagcattaat ttaaatgcta
     5881 ccggatatat ttttggagcc cgattcacat gggaaccaag aaaaaacaaa acggaacaca
     5941 aaaaaccagt gatcacgaat tgcagagggg ctccaaatag gtgtgccatc acgtgcaccc
     6001 gcggcggtgg ggggagcgaa tgactccaaa gatacgcggt tgccaacgct gggattagac
     6061 taagggcacc taaaaataca ccgtattggg cgtcgttaga atgaatatca cccgaaaaat
     6121 ctcgctccca ggagcgggtt cccgagagaa ctccgccctt gttggccgaa aaattctctt
     6181 taaggtcccc caaccagcgc ctaaacaatt tggtcattta gtacctatgc cataagacac
     6241 gcttgccgtc attaccccat cggtgtaaca gtggattctc attctatatg ataaaaaacc
     6301 tcaagcgtcg cctattaggc tcgcccattg catcggccct cgcccatagc gaacgcatca
     6361 ataaacgccg ggcgctcgcg gtattttcct ctgacgccct ctcgtcggta gcgtatgcta
     6421 ctcaagaaat cttattggta ctagtgcttg ccggtggagc agcgctctct tattcaatac
     6481 ccatttctgg cgctatcatc tgtttacttt ttctcgtagt agtatcctac cgccaaacga
     6541 ttcgagagta tccgaacggc ggcggggcgt tttcagtaac caaagaaaat cttggcccgt
     6601 cgtggggcct ggtggctggt gcggcgctct taatagatta cgtactgact gtcgcagtga
     6661 gcgtcgcggc aggtgtagcg gcacttactt cggcctttcc ctctttgttt cagtataaaa
     6721 ttacaatttg tgcagtggct attggtgtgc ttacaatcgc caatctacgt ggggtacgag
     6781 aatccgccac tattttcgcg tttccgacct atggcttcat cgtcggaatt ttgtcgatgt
     6841 tggccattgg cacctacaaa ttgtacaccg gtgaattcac tcatatcgac cctcctgcta
     6901 ctgagggcct ggaacctttg acggtttttt tactattgca tgctttttct tctggatgca
     6961 ctgccatgac tggagtggaa gcggttgcta acggtgtgcc ggtatttcag cctccggaat
     7021 caaaaaatgc atcgaagacg cttataatca tggcctgttt gctcacaacc atgttttttg
     7081 gaatcactta cctttcccac taccatggca ttgccccgaa cgaacaagag accgtgttgt
     7141 cacaattagc ccgttccatt tacggcagca cctggcccta ttactcgata cagatattaa
     7201 ctatgggtat tctcttttta gcggccaata cgagttacgc agcttttccg cgactcggtt
     7261 cgcttctggc aaaagatcgc taccttccac gacagtttgc cagtttgggt gatcgcctag
     7321 ttttttcaaa cggcattcta atactcggag tagtctctac cggattgatt tacctgttcg
     7381 atgctgacac ccatgctttg attccgttgt acgcagtcgg cgtattttta tcattcacgc
     7441 tttcccaggc cggtatgttg cgtcactggg aacgttcacg agcccgcggc tggattcggc
     7501 acgcaattgt ttcgggcata ggcatgaccg tcactgcggt cgtaactaca atcattctca
     7561 ccacgaaatt taccgatgga gcttggctca ttacgattgc cgttccggct ttgataattt
     7621 tatttcgcca agttcaccac cactaccttt cggtgggtaa acaactttct cttgtcggcg
     7681 tagaccccaa atcgcttccg cacgaactta aacacaccgt tttgctgccg atttcgggtg
     7741 tgcatcgcgg agtaatcgaa gcgatgcgat acgcacaatc cattgcgact gacgttagag
     7801 cggtttacgt ggaaatcgat aaagaaagta ccgagcggtt tcaacaagat tggcaacggt
     7861 ggggccgtgg agttcctctt gttgttttac ggtcaccgta tcgctctgtc gtaacacccc
     7921 tacttcgtta catcgacgaa gtggaagatt ctaatcaaga cgacatgatt actgtgatta
     7981 ttcctgaatt tgtgacagcc aaatggtggc aacaattctt acacaaccaa actgcattaa
     8041 ttattcgtgc tgctttattg ttcaaacgca ataaggtcgt aacaagcgtt cgctatcatt
     8101 tggattaacc cttaaactac agggtgccag ttttaataaa attcgaataa tttttggcac
     8161 gatacaattg gtacttgcct agcaaataac acagtataga aaatccgagc acttcgccgc
     8221 ccaaggtata cggcgttttt agaacatcgg tccaccttct caccaaatcg ggctcatgcc
     8281 actgcacggg accgccaaag ggaaaataaa aaatgtcggg gctctttatc cacatttgat
     8341 cgagcaagag gtgcgaacaa taaaataaag cgtaaagcat gattgccgac cttcggcgag
     8401 agaacgccag cagcaaactc acagcaaaaa ctatggcaaa aagattgtgg ccaattagcc
     8461 gagtattgaa tcgaaaaaat tccggcgaac taagaccaag cggcttatct ataagatcag
     8521 cgctcatcga cattaacgcc acataacgca ggtctatttt ttttttgaat ttcttattga
     8581 tccagtgtgc ggtaccgact gcaagaccag tatgtccaaa aagaaacata ttttttaggc
     8641 tggaaaaact accacaattt ttccaaaaac ccctcgcgat tcgattt
//
LOCUS       DAOMXO010000012         8661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_4293_length_8661_cov_5.023123, whole genome shotgun sequence.
ACCESSION   DAOMXO010000012 DAOMXO010000000
VERSION     DAOMXO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4293_length_8661_cov_5.023123"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..399)
                     /locus_tag="PLH57_00550"
     CDS             complement(16..399)
                     /locus_tag="PLH57_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01126.1"
                     /translation="MIHCKRAMVPERLSLLDGKVLKEKKNRRGALVWTVEFTRPDGTP
                     VTGKREGFQHLYGMKKPGDHVPILNVEGAPECWDISTQADRPAAYEGRQNFAITISGG
                     FGVFFILSGAALSVWSFRKLFFSPV"
     gene            complement(509..889)
                     /locus_tag="PLH57_00555"
     CDS             complement(509..889)
                     /locus_tag="PLH57_00555"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HRK01127.1"
                     /translation="MKLAFHLAERSTCVRLQVGTVVTSTDYRRVLAVGYNGNATGLHN
                     GCDRTEPGNCGCLHSEENAVINCDAPRQVEKIVFVTHLPCVQCAKRLINLGNVKRVYY
                     ANDYRIRDSIAVLQSVGIEIEKLE"
     gene            994..1803
                     /locus_tag="PLH57_00560"
     CDS             994..1803
                     /locus_tag="PLH57_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRK01128.1"
                     /translation="MASQPRRTTKRTATQILSWNVNGLRAVGRNGFFDWLADTSPDVL
                     CLQEIKCVVGDLEDAQVMPLGFRSYWHSAEKPGYSGVAIYTKHAPVRVIEGIGVPDID
                     REGRVLSLEFETCIVVNAYFPNSQREHERLPYKLHFCAELEKFLKKLRQSGKPVLLCG
                     DYNVAHREIDLRNPKTNVNNAGFLPAERAWMDAFLSAGWTDTFRHFVREGDHYTWWSY
                     RPGVRERNIGWRIDYFCVSSEAISKVKDAFIWPDVIGSDHCPVGVEWDGEL"
     gene            1800..2390
                     /gene="orn"
                     /locus_tag="PLH57_00565"
     CDS             1800..2390
                     /gene="orn"
                     /locus_tag="PLH57_00565"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HRK01129.1"
                     /translation="MTNQNGEKKTALPESNGDSQAMKKLFWIDLEMTGLDEKVDQILE
                     VAVLITDLDLKTLDSYHRVVFQAPEVLENMNDWCKKTHGKSGLSADVPGGMPLKKVEQ
                     ELIELANKHYPNPKDRIVLCGNSVGNDKRFIDAYWPDFAKKLHYRLVDVSSFKEIFKA
                     RYNIQYKKSDEGRHRAEDDIVQSVKELAHYLSFVKP"
     gene            2392..3075
                     /locus_tag="PLH57_00570"
     CDS             2392..3075
                     /locus_tag="PLH57_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01130.1"
                     /translation="MAIFTALFAATLSTNAALVEGAPKWATLAKINRVADRIERALEW
                     DIRRVRVRWFSNADQFAQAHNLKGDGTANILAVTHKQRGEILIGPLVTPDKFEGVLGH
                     EFAHVIIYQKYKTAIPPWLEEGLANQVAGRKTVEWKAVRIFLEANPEFDVRTLGHPFK
                     TETANLSVALQYSISTALMQTIASRCRVYDLLQMSVGYSVDTYLKNLCQIDDLNTAFR
                     RYVFKNAGA"
     gene            complement(3072..4343)
                     /locus_tag="PLH57_00575"
     CDS             complement(3072..4343)
                     /locus_tag="PLH57_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRK01131.1"
                     /translation="MQKSFFAVTAKGLEPALAEELRALGALNIEERPGGVAFAGNQET
                     LYKACLWLRTATRVLQEIRTFSARSPEMLYDQIRRLKWHELLTAEMTFAVDCVASGTR
                     VKRESLDEDFENAPPRPLASPSIAPTSPSFAHRRLQPHAVDNRLQPRFVALKIKDGIA
                     DEMRKRLNGVRPSVDAENPDVLIHAYLNDGKCVLSIDASGASMHERGYRKQDSGAPLR
                     ETLAAGLVLMTEWPKDTSQPFIDLMCGGGTLCIEAALIGANIAPGLLRTKRFGFETWR
                     DFDKALWNRLRSEAVAARSTTKLNIQGFDMNELAIEAARSNARRASVDKLVRFELGRI
                     ENLPSNRVPTTAGTVIVNPPYGERMGAADRLKPLYKRLGDAYKHQFKGWKGFIFSGNS
                     DATKSVGLRTSRRIPLFNGPIECRLLRYELY"
     gene            4412..5185
                     /locus_tag="PLH57_00580"
     CDS             4412..5185
                     /locus_tag="PLH57_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRK01132.1"
                     /translation="MSFATCVWIFFAVLVDGLAGLVGGFLPAAWVQRHLAALLAFASG
                     TLVSVALLDLLPEALEHISSLDSFTPIMHSGAEDVMVSVLIGFVTFYVIESFLGSHAS
                     GQSGHKHDTVGPLILVGDAVHNAADGVAIAAAFAVDVKVGIGTAIAVVVHELPQEVSD
                     YAILVSHGYSKRKALLSLFVVQMFAMVGAGVAIVAFRFAEVAAVYLMALSAGGFIYIA
                     AADLLPELHRRKSGAPILKLVSFGAGVAVIAITRSIFSH"
     gene            5213..6214
                     /locus_tag="PLH57_00585"
     CDS             5213..6214
                     /locus_tag="PLH57_00585"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRK01133.1"
                     /translation="MKRTIVLSLAICTLFTAPVLAEEETPPPPPEFAAKIQAVEGNPE
                     VRVISIAASDQRMAKQGDSVDEGEKIVTEAGQTVILQLAENTEIAIGPATSFTIEQVS
                     KEGPQQSILGLAYGVIRAFVSRQYGQDENFAVKTKNSVMGVRGTEFIAETSAITEETE
                     VHTLSGEVRFANGTTDLKDLRTAMAVPAGHASRLARGSASPSAARKFDRAKFQKMLKT
                     RMPAFERRVRAFNPGLGAHGRFQSLKKGTRIERVQNPNAGISKGNVRATMERKLQKRL
                     EKNRDQAARKRELRNQRREQMSKRDKNRRMGPKPGVPGMLPPPPPPPPPPAGGGVSY"
     gene            complement(6706..7227)
                     /locus_tag="PLH57_00590"
     CDS             complement(6706..7227)
                     /locus_tag="PLH57_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01134.1"
                     /translation="MTKHANMVCIHCHLAKPRTDKCELCEESVCASCVIRPTPERVQY
                     WSSTDRPKETECKWVCPRCFDETLEPIYERYDQALEAAHNTHYFPKAYRGAPPVLRKA
                     RQPVTVDNGVDREDVLTKLQLVAVQQGFNGLIHLEIESKKQRMVDGYSKMVWRGTALP
                     ADLDTEKLERGWY"
     gene            complement(7227..8210)
                     /locus_tag="PLH57_00595"
     CDS             complement(7227..8210)
                     /locus_tag="PLH57_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01135.1"
                     /translation="MKSIFKTPSFGPVGALFVLWALTAPLCLAAYGATINCREILVAY
                     SEPLARLLRADAQSAYLQIVENGRARQIELTSHLEKENRANDLTLPYLKIVHSEALPI
                     QLVLEAGDAPLDFFQATAKGRALRRNVELFERKKRNFLSAMQESIGDNGDRMREMKIT
                     RTTLLSLMDHWRKKNSDLADAMAFLIYEIRHRRGIPYGLGEFIDSVEATGIDEIVAEN
                     ILRGTYGETDRAAAIAEEVGIAQIRIAGTLLQARRIRDQRMGDEAARKEEEEKARISK
                     KLPPRAEQIHRAAIVSAANGSSKGKVQYYSDELIGSVTVSGEIPTRSQGNK"
     gene            8402..>8661
                     /locus_tag="PLH57_00600"
     CDS             8402..>8661
                     /locus_tag="PLH57_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01136.1"
                     /translation="MGIFARSLLLFGLVIVGASAGAMNSFAASGACDLWGYLRKAAVV
                     KRPQVQGSVPKIKSIDLPPWLADDYQVLILKDKNFPEISEPV"
BASE COUNT         2074 a         2113 c         2321 g         2153 t
ORIGIN      
        1 ggaaatttca gattttcaaa caggggagaa aaagagcttg cgaaatgacc acacgctcag
       61 tgcggcgccg gataagatga agaacacccc aaaaccaccc gaaatggtga tcgcgaaatt
      121 ttgacgcccc tcgtaagcag ccgggcgatc agcctgagtg ctgatgtccc agcactcagg
      181 ggcaccttca acatttagaa ttggtacgtg gtcacccggc tttttcatcc cgtacaagtg
      241 ttgaaaacct tcgcgttttc ccgtcacagg agtgccgtct ggccgagtga attctacggt
      301 ccacaccaac gcgcctcggc ggttcttttt ctctttgagt actttgccgt caagcaaact
      361 caaacgctcg ggcaccatag cgcgcttgca atgaatcatg tgcaatgcgt cgatgctcag
      421 caaaattcca aagccgaaca ccaaaaaaaa agatccgcaa aaccaaatcc acttcaatgg
      481 acggtcgcca tctggcgtac cgttagtcct attcgagttt ttcaatttca atacccacgg
      541 actgaagcac cgcgatcgaa tcgcgaatgc gatagtcgtt tgcgtagtag acgcgtttaa
      601 cgtttcctaa gttaattagt cgtttcgcac actgcacgca gggcaggtga gtgacaaata
      661 cgattttttc aacttgtctc ggtgcatcgc aattaatcac cgcgttttct tcagagtgca
      721 ggcagccgca gtttcccggt tcggtgcgat cgcagccgtt gtgcaaaccg gtggcgtttc
      781 cattgtagcc caccgcgagc acgcgccgat agtccgtact ggttaccacc gtgcccacct
      841 gcagccgcac gcaagtagag cgttcggcca agtgaaacgc gagtttcatg taaatgcttt
      901 cgaatttagg gcgtaaaccg gttgggcgta cgagtggttg attttggctt tcggttgcgt
      961 tcatagagtt ggagtattac aagttttggg actatggcaa gtcaaccacg gcggacaacg
     1021 aagcgaaccg ctacccaaat actctcctgg aacgtcaacg ggcttcgtgc ggtgggtcgc
     1081 aatggttttt tcgattggct cgccgatacc tcgcccgacg tgctctgttt gcaagagatc
     1141 aagtgtgtgg tgggggatct agaagatgcg caagtcatgc cgctcgggtt tcgaagttac
     1201 tggcatagtg ccgaaaaacc agggtacagc ggtgtggcaa tttacacgaa acatgctccg
     1261 gtgcgggtga tcgaaggcat tggggtgccc gacattgacc gcgaagggcg tgtgttgagc
     1321 ttggagtttg aaacctgcat tgtcgtgaac gcctattttc cgaattccca aagagaacat
     1381 gaacgactgc cctataaact gcatttttgc gccgaacttg agaagtttct aaagaaatta
     1441 cgccaatctg gtaaaccggt attgctctgt ggggactaca acgtagctca tcgcgaaatc
     1501 gatctacgca atccgaaaac caatgtaaac aacgcgggat ttttgcctgc agagcgcgcc
     1561 tggatggacg cgtttttgag tgcgggctgg accgacacgt ttcgccattt cgttcgggag
     1621 ggcgaccact atacttggtg gagttaccgc cctggtgtgc gcgagcgaaa tatcgggtgg
     1681 cgtattgatt atttctgcgt gagcagtgaa gctatttcca aagtaaaaga cgcatttatt
     1741 tggcccgacg ttatcggcag cgatcactgt ccggtaggcg tcgagtggga tggagaactg
     1801 tgaccaatca aaacggcgaa aaaaaaacag ccctgcccga atccaatggc gattcccagg
     1861 cgatgaaaaa acttttctgg atcgatctcg aaatgacggg actcgacgaa aaggtcgatc
     1921 aaattttgga agttgctgtg ctaattactg atttagattt gaaaacactc gatagctacc
     1981 accgagtcgt ttttcaggcc ccagaggtgc tcgaaaacat gaacgattgg tgtaaaaaaa
     2041 ctcatggcaa gagtggactt tcggcagacg tacctggagg aatgccgctt aaaaaggttg
     2101 aacaggagtt aattgagctc gcgaacaaac actatcccaa tcccaaagac cgaatcgtgc
     2161 tttgcggaaa tagcgtggga aacgacaagc gttttattga cgcttattgg ccggacttcg
     2221 ccaagaaact tcattatcgt ttggtcgatg tttcttcgtt taaggaaatt ttcaaggcgc
     2281 ggtataatat tcagtataaa aaatccgacg aaggccgaca ccgagcggaa gacgatattg
     2341 ttcaatctgt aaaagagttg gctcattacc tctcgtttgt aaaaccctag tatggccata
     2401 ttcactgcgc tttttgctgc aactttatcg accaatgccg cgcttgtcga gggagcgccc
     2461 aagtgggcta cacttgctaa aatcaatcgg gtggccgacc ggattgagcg ggcgctcgag
     2521 tgggacattc gcagagtaag ggttcgctgg ttttccaatg cagatcaatt cgcgcaagcc
     2581 cataacctca agggcgacgg tactgcaaat attttggcgg tcacccataa acaacgaggc
     2641 gaaatcctaa ttggcccctt ggtcaccccc gataaattcg aaggtgtatt ggggcacgag
     2701 ttcgcccacg tcattattta tcagaagtac aaaactgcta taccaccgtg gctcgaagaa
     2761 gggctcgcca accaggtcgc cggccgtaaa acggtggaat ggaaagccgt acggattttt
     2821 ttagaggcga accccgaatt tgatgtgcga acgctggggc accctttcaa aactgagacg
     2881 gcaaacttaa gcgtggcttt gcaatattca atttccacgg ccctcatgca aacgatcgcc
     2941 agtcgatgcc gcgtttatga ccttttgcaa atgagtgtcg gttatagcgt agatacgtat
     3001 ttaaaaaatc tctgtcagat tgatgatctc aacactgcgt ttcgcaggta cgtatttaaa
     3061 aacgctggcg cttaatacaa ttcgtagcgc aacaaacggc attcaatcgg gccgttaaaa
     3121 agaggaatgc gccgagaggt gcgtaggcct accgatttcg tggcatcaga gttgccgcta
     3181 aagatgaatc ccttccaacc tttgaactgg tgtttgtagg catcgcccag acgtttgtag
     3241 agtggcttta agcgatctgc agcccccatg cgttcgccgt agggtgggtt cacaatcacg
     3301 gtgcccgcag tagttggaac tcgatttgac ggtaaatttt caatgcgccc aagttcaaaa
     3361 cgtacaagct tgtcgaccga ggcgcgtctg gcgttggagc gggccgcttc aattgcgagc
     3421 tcgttcatat cgaatccttg gatattgagt ttggtggtcg atcgtgcggc tacggcttca
     3481 ctgcgcaggc gattccaaag tgccttgtcg aagtcgcgcc aggtttcgaa gccaaaccgt
     3541 ttggtgcgca gaagtccggg tgcgatattg gcgccgatga gtgcggcttc gatacaaagc
     3601 gttccaccgc cgcacataag atcaataaac ggctggctgg tgtccttggg ccactctgtc
     3661 atgaggacta gtccggcggc caaagtttcg cgcagcggtg cgcccgagtc ctgtttgcga
     3721 taacctcgct cgtgcatgct ggcgccactg gcatcaatac tcagtacgca cttaccgtcg
     3781 tttaaatagg catgaattaa aacatcgggg ttttcggcgt caacacttgg gcgtacgccg
     3841 tttaagcgtt ttcgcatttc atcggcaata ccgtctttaa ttttaagcgc cacaaagcgg
     3901 ggctgcaggc ggttgtccac cgcatgcggt tgcaaacggc gatgcgcgaa gctagggctg
     3961 gtgggcgcga tgctagggct cgccagaggc cgcggcggtg cattttcgaa gtcttcgtcc
     4021 agggattctc gttttacccg agttccactg gcgacgcagt ccacagcaaa agtcatttca
     4081 gcggttaaaa gctcgtgcca ttttaagcgg cgaatctgat cgtagagcat ttccggcgag
     4141 cgcgcagaaa aggtgcgaat ttcttgcaac actcgagtcg cggttcgaag ccaaaggcag
     4201 gctttgtaga gggtctcttg gttgcccgcg aaggctacgc cgcccgggcg ttcttcaatg
     4261 ttcagggcac cgagagcgcg aagctcttcg gctagtgccg gttcgagacc ttttgccgta
     4321 accgcaaaga atgatttctg cataggggga tgacataccg cattctgtat tttttgacaa
     4381 atatagtttc ctattataac acctgttttt tatgagcttt gccacctgtg tatggatatt
     4441 ctttgcggtt ttagtcgatg ggcttgcggg gctcgtcggg gggtttctcc ccgccgcctg
     4501 ggtgcagcgc catttggcgg cgcttttggc gtttgcttcg ggcacgctag tctcggtggc
     4561 gcttctcgac ctgcttccgg aggcgctcga gcacatttct agcctggatt cgttcactcc
     4621 gatcatgcac tcgggggccg aagatgtaat ggtttcggtc ctcataggtt tcgtgacttt
     4681 ttacgttatt gagagctttt tgggcagcca tgccagtggc cagtcgggtc ataaacacga
     4741 caccgtgggt ccactcatct tggtgggtga tgccgttcac aacgccgccg acggtgtggc
     4801 gattgccgcg gcttttgcag tggacgtcaa ggtcggcatt ggtaccgcga tcgcggtggt
     4861 ggtgcatgag ttgccccagg aagtgagcga ctacgcaatt ctagtttccc acggttactc
     4921 taaacgcaag gcacttttga gtttgttcgt ggttcaaatg tttgccatgg tgggagccgg
     4981 tgttgcgatc gttgcttttc gtttcgctga agtggctgca gtttatttaa tggcactctc
     5041 agccggtggg ttcatataca tcgcggcagc ggatctcctg ccagagttgc accgcagaaa
     5101 atcgggcgcc ccaattttaa agctcgtttc ttttggtgcg ggtgtcgcgg taattgcaat
     5161 aacgcgcagc attttttcgc actaacccta ttttcgcgtt accattgaga ttatgaaacg
     5221 cacgatcgta ctctcgctcg caatttgcac gctttttacg gccccggtcc ttgctgaaga
     5281 agaaactccg cctccgccac cggaattcgc cgctaaaatt caagcagtgg aaggcaatcc
     5341 agaagttcgg gtgatctcga ttgcagcgtc cgatcaacgc atggccaaac aaggggactc
     5401 ggtcgacgaa ggagaaaaaa tagtgactga ggcggggcag acggtcattc tgcaactggc
     5461 cgaaaacacc gaaatcgcca ttgggcccgc tacgagtttc acgattgagc aggtctccaa
     5521 agagggacct caacagtcga tcttaggatt ggcctacggt gtgattcgtg catttgtcag
     5581 ccgccaatat gggcaagacg aaaactttgc agtgaaaact aaaaattcgg tcatgggagt
     5641 gcgaggcaca gagttcatcg ccgaaacgag tgcaatcacc gaagaaacag aagttcatac
     5701 actctcgggg gaagtgcgtt ttgccaacgg cacgaccgat ctaaaagatt tacggaccgc
     5761 catggcggtg cccgctggac acgccagtcg cttggcacga gggagcgcaa gcccgagtgc
     5821 ggcacgcaag tttgaccgag ccaaatttca aaaaatgctt aaaactcgta tgccagcttt
     5881 tgaacggcga gtacgggcgt ttaatccagg gttgggcgcc cacgggcgtt ttcaaagcct
     5941 caaaaaaggt acccgcattg aacgagttca gaatccgaat gctggaattt caaaagggaa
     6001 tgtgcgggcc acgatggagc gcaagctcca gaagcgactt gaaaaaaacc gagatcaagc
     6061 agctcgcaaa cgggaactcc gaaatcaacg acgcgaacaa atgtctaaac gagacaaaaa
     6121 tcgcagaatg ggacccaaac ccggcgtgcc aggaatgctc cctccgcctc cgcctccgcc
     6181 cccaccaccg gctggcggcg gcgtgagtta ttaataagtt cccggacttc gtcctgcgta
     6241 gagaccaccg attcttttac aggcggtggg gcccgtgcat tgaatcacgc ttaagatacg
     6301 agttcggcta tcgcacaaga gcgcttcttt gcgggtaggt gtttggaacg gacaaaaact
     6361 cgctgacaat ggtcgagcat ttccggcagc gaacgatagc gcgtaagtct gaacgtccga
     6421 tgagataagt tcgatttggt taaattgtgg agccgagcca tttttgggtg ttgtgctcgt
     6481 ttcgacaaac aaaagatttg attgcttact ttcacttgtg gctgcgcttg tagcgacaat
     6541 ggctaagaca aaaaaaacaa aaagtggcca gcgttggggt gacatccgtg tcgccctcct
     6601 ttgcgatcat tatggctggg aagaaaaaaa aagaaaagac cggtgaaccg gatttcaaaa
     6661 ggcaaaaaat gcttctattt gaaccgaaaa gaggctcgcg ctacttcagt accagccgcg
     6721 ctctagcttt tcggtgtcga ggtcggctgg gagtgccgtt ccacgccaca ccattttcga
     6781 atagccgtcg accattcgtt gttttttact ttcaatttcc aggtgaatca acccgttgaa
     6841 gccttgttga actgctacca actgaagttt agtcagcaca tcttcgcgat caacgccatt
     6901 gtctacggta acgggctggc gtgctttgcg cagcaccggc ggcgcaccac ggtaggcttt
     6961 tggaaaatag tgagtattgt gagccgcctc aagggcctgg tcgtagcgct cgtaaatggg
     7021 ctctagcgtc tcgtcaaaac aacgagggca aacccatttg cattcggttt ctttggggcg
     7081 atcagtagag ctccagtact gtactcgttc aggcgtgggg cgaatcacgc aactcgcaca
     7141 gaccgattct tcgcaaagct cgcacttgtc ggtgcggggt ttggctaaat gacaatgaat
     7201 acaaaccata ttggcatgtt tggtcattat ttatttcctt gggagcgtgt gggtatttca
     7261 ccggaaacgg ttacgctgcc aattagttca tcggagtagt attgaacctt acctttggat
     7321 gatccatttg ccgcagatac aattgctgcg cgatgaattt gctcggcgcg agggggaagt
     7381 ttttttgaaa tacgagcctt ctcttcttct tcttttctgg ctgcttcgtc tcccatgcgt
     7441 tggtcgcgaa tgcgtcgagc ctgcagaagg gtaccggcaa tccgaatttg agcgatcccg
     7501 acctcttcgg cgatagctgc ggcgcggtcg gtttcgccgt aggtgccacg caatatgttt
     7561 tcggcaacaa tttcgtcaat acctgttgct tcgaccgagt cgatgaattc gcccagccca
     7621 tagggaatac ctcgtcgatg ccgaatctca taaattagaa atgccatagc atcagccagg
     7681 tctgaattct tcttgcgcca atggtccatg agcgagagaa gagtcgttcg ggtgattttc
     7741 atttcgcgca tgcggtcacc gttgtcgccg atcgactctt gcatcgcact taaaaaattt
     7801 cttttctttc tttcaaatag ctcgacattt cggcgcagtg ctcggccttt cgcggtggct
     7861 tgaaaaaaat cgaggggcgc gtcgccagcc tcaagaacga gttgaatcgg caaggcctcg
     7921 gagtgaacga tcttgagata aggtaaagta agatcgttgg cgcggttttc tttttcaaga
     7981 tggctcgtaa gttcaatctg ccgagctctt ccattttcga cgatctgtaa atatgcactc
     8041 tgcgcatcag cacgaagcaa acgggccaag ggttcactgt acgcgaccaa gatctctcga
     8101 caattgatag tggcgccata ggcagccagg caaagtggtg cggtgagcgc ccagagaaca
     8161 aacagggcgc caacaggccc gaatgaagga gttttaaata tcgatttcac gcggtgcgtt
     8221 gtatataatt tccgacgaat tagggcaact aaaatgtgcg cgaattcgtt gattatatga
     8281 ggtttttccc tgtttagcct ctaacgcctc gggattacgt ggtttttttg gttttatata
     8341 ttgattaaat tagtcaacta atgagacaat ggctaataga cgagtcttag ggggacgttt
     8401 cgtggggatc tttgcccgca gtctattgtt atttggtctt gtgatcgtgg gggcttctgc
     8461 cggtgcgatg aattcattcg ccgcaagtgg tgcctgcgac ttatggggct atctacgtaa
     8521 agcggcggtg gtgaaacgac ctcaggtgca aggatcggta cccaaaatta aatcgatcga
     8581 tttgccccca tggctcgccg atgattacca agttctaatt ctaaaagaca aaaatttccc
     8641 cgaaatttcg gaacccgtct t
//
LOCUS       DAOMXO010000013         8650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_4299_length_8650_cov_4.512391, whole genome shotgun sequence.
ACCESSION   DAOMXO010000013 DAOMXO010000000
VERSION     DAOMXO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4299_length_8650_cov_4.512391"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PLH57_00605"
     CDS             <1..854
                     /locus_tag="PLH57_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01137.1"
                     /translation="HPFMALDVNDLKEPAAIDSFIAHELHHLFQISYSWGLPTTIHEM
                     SANYFQEAFSPGQNHKAFSEVQAFQARPEWPVFLEDFESDFMYGAWLYFKFLELNYFE
                     SRPEFLRNFWEQFRQADRGTRKYEQEDLVQALTNVLPGSISHDEALLEFALWRTFLFP
                     GAREQSLEISERHFGGMKLPGPKFMHRIDARDDRVIGKIEQVYGTHYVEVANLTAFEV
                     ELTLNGSPDVRWVLQQVTFGDRMIAAITALPHYAGPAPSSLSYELKIRTIWGGRSLFD
                     RRLLSSK"
     gene            985..4611
                     /gene="smc"
                     /locus_tag="PLH57_00610"
     CDS             985..4611
                     /gene="smc"
                     /locus_tag="PLH57_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRK01138.1"
                     /translation="MRVKRLEIQGFKSFKDKTVIHFDDGVTGIVGPNGCGKSNIVDSF
                     FWVMGEQSTKHLRGSGQGDLIFNGSEKYSPSGMAEVTMVLSTGAKGAEIPEGMTIRDL
                     PAHLRFEEIAITRRLFRDGESEYLINGQTCRLKDIHELFMDTGAGPKAYSIIEQGQIS
                     RIVQQKPEERRTLIEEAAGIVKFKARKKESLRKIEATQQNLLRINDIVAEIERQLGSL
                     ERQATKARQYKKYKEELQGKELLSGRKKLFGLKKRSLEVAGRVQELEITDVERRNSLQ
                     VTELQIENLKLQMTEAQRLAEETQQQLQILQREISQDDTRIQLHRRNIQELENSNLTL
                     DEEREELISDIERLSAEHSALENEALELEALFKNADEMLREQQAQLDSAKLAADELGR
                     LVEQEKRDLMASLNRRNEISNQVHGLEAKVDSLSVQVNSLNLRIADREVDTELLTEKA
                     SAARELYQTSATQADALRVEVEGLASSIVDLDTRLGALRKEDSVAREEKAKTESKHKT
                     IQELVDNHEGFRADVQAILKDESFAGSITGVFADRLEAHPGYEYALEGVLREFLESLF
                     VENATKAREMIAVLKEQSVGRATFWPADLVAKRSISSPNSDLVSTLRSRGIEATALAE
                     VIRLDAPNSAELLKLFDRYWVVASLDDAVTAFEAAADLGLCFVSREGDVVDSFGRVAG
                     GSVKSLETGILARKAELQRLTELLTEWTGKCQLLSESLAAVESDLIEKKSIHATKSAQ
                     LREVDLAAKSAERDLASLESQLSSIQSELERQKNEREQLEAGRRDLWQKLDSIRQELS
                     SMEDSTRARESVIEEKSRAHNQSVIKVSEIQGAMIQMKVDFAAANEKHRNSKQNRERV
                     VSQLSKDKSRLEEVERMINRKLDEKDLVASELGSLETRMQELTVKAQELEQLLASRKN
                     ELEKTNHMMSDSFEKQREALRNVEEASSELSRLKLEQERSQLEYQMVYQNLFEKYGVE
                     ESAIAPQNDEDQRAFDELTPERETEMSEEVERLRERIRKLGEVNVMAVEEYDEQKKRH
                     DFLMTQREDLLKSIHDLEKAIERINKTSEERFRIAFEEINSRFQRIFPIVFGGGGARL
                     EMTNPADLNETGVEILAEPPGKKIGSIQLMSGGEKALTAVALIFSIFLIKPSPFCVLD
                     EVDAPLDDHNVGKFNMLLKEMAIRSQFIIITHNKRTMELNDKLYGVTMEEPGVSKMVS
                     IQLQ"
     gene            4740..6401
                     /locus_tag="PLH57_00615"
     CDS             4740..6401
                     /locus_tag="PLH57_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01139.1"
                     /translation="MSKLRVGPATEQYAQVASQRLETATAIKQGPPAPGTPLAVPWET
                     AHAQVNMALNWAQSMAPTRDGIYVVGPVSVKNPPVKPVSSSWGMRSFWSLVLFAGATV
                     FSGCFGSKIVPAGNTDSVVNTPADIGPSNNAVCNPFGNANHAGAGYAHGVVGKLQYLT
                     DDMPRYNNLNDFMNNAQAVDASIYFGKIDIPTRPFDRGFITLDGEAILNSSGTTLYEY
                     FSLRLESMLRLQDASAPKKYQFGLLADDGAILSADFGSGYETIINNDGTHPSKLMCST
                     SPVELDFTSALPIRIDYYQGPRYHIALVLVWREWSDDAAFDPNDPLCGKQGNGHFFKF
                     AENPPSPQPAWVNLLARGWSVVPAEVFELPSNIASNPCDAVESMTTTLTSISPNSEFT
                     RSPDITFEFESNRSGALFQCSIDGGDFSSCTSPKTYINVGTGAHTFTVRAIDDIGNFD
                     EIGASHSWTVDQSVPTIVVTASSVTSNSITVTWTTNEPATTSLAWGLGGDTSQIIPDD
                     GVYDTNHSVTLTGLTADTVYSYRMISTDQAGNTMTSSRRVVRTSP"
     gene            complement(6372..7874)
                     /locus_tag="PLH57_00620"
     CDS             complement(6372..7874)
                     /locus_tag="PLH57_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01140.1"
                     /translation="MVTITRVFVLVICVLAVRTHAYSISGGQLVEDFTSLDGADLTNT
                     TGLWNTVTSKAEAGRVADAGGGTHRPISFGDGSDGILDTSGGYSFDTDAKPNGYNFKR
                     VNITGGTITVTGSNPLIIRSLTTVNITPTIQVNGTAGSDGVANGSSTAPSGGTAVTCP
                     AVGGAGGTASAGAVGNGADGVIYTGATDASGGLGQVNGTNGDDGGATSALPGANFDNA
                     TAFICGGGGGGGGGHRNTVGFQYATGGAGGAGGGTVRITAVGSLTVGTIEAEGGNGGN
                     GITSDGANCSGAGAGGHGGAVWLQTLADIPLAIPAPSVTNGTGGTSACGGAASDGTVG
                     KIRLDSSPGNRPAHSNVADYDTDDVFPSRSYVIQSAAYDLGTLNANFKTAPTVTQTLN
                     SGTISLSYAGSVDGSVFSDYESDITKLSGKNYRYLKFKINITTAAIAGASPEVSRIVI
                     DFADSGLAKVDLKLTAGCGTIQNGESGWIFILLFLTAAYFRLRARTNHAT"
     gene            complement(7858..>8650)
                     /locus_tag="PLH57_00625"
     CDS             complement(7858..>8650)
                     /locus_tag="PLH57_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01141.1"
                     /translation="ILKHQTVKTRFILQTRQSPKERSMYFRVLAVDRGGKEGEFSPTK
                     HILIEPYQDPTLARLNRIRIGIAVGSIYHSRKLNSDSNPETATASGFVPALVDLTVRK
                     RKNDSTDWFMGLSIIAEQGEPTNKNVLVEKVALPLARITLGQERDISSERALTYGLYA
                     SNTHELSWKGRSLKSERKILLGAVLGIGQSQKRPLRSTFSASIAPFAMTGIGADISAK
                     AKMRFGPLNTNAHLSSHFFSEAELLGRISTKEISYGGAIKIGYYY"
BASE COUNT         2269 a         2029 c         2190 g         2162 t
ORIGIN      
        1 tacatccgtt tatggcgctc gacgttaatg atttgaagga acccgcagcg attgattcgt
       61 ttattgctca cgagcttcac catttgtttc agatttctta tagttggggg cttccgacca
      121 caattcacga aatgagtgca aactattttc aagaggcttt tagtccgggt caaaatcata
      181 aggctttttc tgaggtccag gcttttcaag cgcggccaga atggccagtc tttctagaag
      241 atttcgaatc ggactttatg tatggggcat ggctgtactt taagtttctc gaactaaatt
      301 atttcgagtc acgacctgaa tttctcagaa atttttggga gcaatttaga caggctgacc
      361 ggggtacgcg gaaatacgaa caagaagatc tggtgcaagc tttgaccaac gtgctgccgg
      421 gttcgatttc ccacgacgag gctttactcg agtttgcgct ttggcgtaca tttctttttc
      481 ccggagcgcg cgaacagtca cttgaaataa gtgaacgcca tttcggtggt atgaagctgc
      541 cgggtccgaa gttcatgcat cgcattgatg cccgagacga tagggttatc ggaaaaattg
      601 agcaagtcta tggcacccac tacgtggaag tcgctaacct caccgccttt gaggtcgaac
      661 tcaccctaaa cggctcgccc gatgttcgtt gggtgttgca acaggtgacg tttggtgacc
      721 gaatgatcgc ggccatcacg gcgcttccgc attacgcggg tcctgcccca agctcattgt
      781 cctacgagct gaaaattaga accatttggg gagggcgatc tttgtttgat cgccggctac
      841 ttagttcaaa atagttcagc gaatagttgc aatctcagtc tagaatcaat acatttttat
      901 caggatttag gttatgacct cgacgaccct tggtcgaatt gatttagttt caacgaccaa
      961 taaggcttct ttcaggagaa cagcgtgcgc gtaaaacgct tggaaattca aggatttaag
     1021 tcttttaaag ataaaaccgt tatccacttt gacgatggcg ttacgggaat cgtaggccca
     1081 aacggttgcg gtaaaagcaa tatcgttgat tcgtttttct gggtcatggg agagcaatcg
     1141 accaaacacc tcaggggtag tggtcagggc gatcttattt ttaatggaag tgaaaaatac
     1201 tctccgagtg gcatggcgga agtaaccatg gtgctctcga ccggcgcaaa aggtgccgaa
     1261 attcccgagg gcatgacgat tcgagatctg cctgctcacc ttagatttga agaaattgcg
     1321 attacccggc ggttattccg cgacggcgag agtgaatatt taattaacgg tcaaacatgc
     1381 aggttaaaag atattcacga actatttatg gacactggtg ccggtccaaa agcctactcg
     1441 atcatcgaac aagggcaaat ttcaagaatt gttcaacaga aaccggaaga acgtagaaca
     1501 ctcatcgaag aagctgctgg tatcgtaaag tttaaggctc gaaagaaaga gtctctgcgt
     1561 aagattgaag ccactcaaca aaatcttttg cgcatcaacg acatcgtggc cgaaatcgag
     1621 cgacagctgg gttctctcga gcggcaggct accaaagctc gtcagtataa aaagtacaaa
     1681 gaagagttgc agggtaaaga gttgctttcg ggccgcaaga aactcttcgg tttaaaaaaa
     1741 cgctcgctcg aagtcgccgg tagggtgcag gagcttgaga tcaccgacgt cgaacgtcgt
     1801 aacagcttac aggtaaccga acttcaaatt gaaaatctta aattgcagat gaccgaggct
     1861 cagcgtcttg cggaagagac ccaacagcag ctgcaaatac tgcaaaggga aatttcccaa
     1921 gatgacacgc gaatccaatt acaccgtcgt aatatacaag agctcgaaaa ttctaatctg
     1981 acactcgacg aagagcgcga agagttaata tcggatatcg aacgactgag cgctgaacat
     2041 agcgcgctgg aaaatgaggc tctagaacta gaggcccttt ttaagaacgc cgatgaaatg
     2101 ctgcgagagc agcaagctca gctagattcc gcaaaattgg cggccgacga attgggccga
     2161 ttggtcgagc aagaaaaacg cgatttaatg gcgagcttga atcgtcgaaa cgaaatttcg
     2221 aatcaagtgc atgggcttga agccaaagtt gattcgttgt cggtccaggt aaatagctta
     2281 aatcttcgaa tcgccgatcg cgaagttgac acagaattgc tgacggaaaa ggcttctgca
     2341 gctcgggagt tgtatcaaac ttctgcaacg caggccgatg ctttgcgggt cgaagttgag
     2401 ggtttggcga gttctatcgt cgatctagac actcgccttg gtgctttacg aaaagaagac
     2461 tcagttgccc gtgaagaaaa ggcaaaaaca gaatcaaaac acaaaacaat tcaggaactc
     2521 gtagataacc acgaaggatt tcgggcagac gttcaggcga ttctcaaaga tgagtctttc
     2581 gccggctcca ttaccggcgt ttttgccgat cgacttgagg ctcatcccgg gtatgaatac
     2641 gctctcgagg gtgtacttcg tgaatttctc gaaagtctat ttgtagaaaa cgcgaccaaa
     2701 gctcgagaaa tgatcgcggt attaaaagag cagtccgtag gtcgggccac tttttggccg
     2761 gctgatttgg tggctaaacg atctatttct tcgccaaatt ccgatctggt ttcaactctc
     2821 cgctcgcgcg gaattgaagc cacagcattg gccgaagtca ttcggctaga tgcgcccaat
     2881 tctgcagaat tattaaagtt gttcgatcgt tactgggtcg ttgcgtcgct tgacgacgct
     2941 gtgactgcgt ttgaagctgc ggcggatctt gggctttgct tcgttagtcg ggaaggggac
     3001 gtagtagact cctttggacg cgtggccggc ggaagtgtga agtctttaga aaccggcata
     3061 ttagcgcgta aagctgagct acagcgcctt actgagctac tgaccgagtg gaccggtaag
     3121 tgtcaattgc tttccgaaag tttagcggcc gttgaaagtg atctcataga gaagaagagt
     3181 attcatgcca caaaaagcgc gcaacttcga gaggtggact tggccgcaaa gtccgcggag
     3241 cgtgatctcg catcgttaga atcgcaattg agttcgattc agagtgagtt ggagcgtcag
     3301 aagaacgagc gcgaacagtt ggaagctggt cgtcgagatc tatggcagaa gctcgattcg
     3361 attcgccaag aactttctag catggaagat tccacccggg cgcgcgagtc tgtaattgaa
     3421 gaaaaatctc gagcccataa tcagtcggta attaaagttt cagaaattca aggcgcaatg
     3481 attcaaatga aagtcgactt tgcggctgcg aatgagaaac accgtaactc aaaacaaaat
     3541 cgggaacgag tcgtatcgca actttccaaa gacaaaagcc gtcttgagga agtcgaacgc
     3601 atgattaacc gcaagttgga cgaaaaagat ctagtggcca gtgagcttgg tagccttgaa
     3661 actcgcatgc aggagctcac cgtaaaggct caggaacttg aacagctgct agccagtcgc
     3721 aaaaatgaac tcgaaaagac taatcacatg atgtcggatt catttgaaaa gcagcgagaa
     3781 gctcttcgaa atgtcgaaga ggcctcgtcc gaactgtctc gcctgaaact ggaacaggaa
     3841 cgcagccagc tcgagtatca gatggtttat caaaatctgt ttgaaaaata cggtgtagaa
     3901 gagtcggcaa tcgctccgca aaacgatgaa gatcaacggg catttgacga attaacaccc
     3961 gagcgcgaga ccgaaatgag cgaggaagta gagcgtttgc gcgaacgcat tcgtaaactg
     4021 ggtgaagtta acgttatggc ggtcgaggaa tacgacgaac agaaaaaacg tcatgatttc
     4081 ttaatgaccc aacgcgaaga tcttttgaag tcgattcacg acctcgaaaa agcaatcgaa
     4141 cggattaaca aaacctcgga agaacgtttc cgaatcgctt tcgaagagat caattcacgt
     4201 tttcagcgaa ttttcccaat cgtatttggt ggcggtggcg ctcgtttaga gatgacgaat
     4261 cccgcggacc taaatgaaac tggcgttgag attctcgcgg aaccaccagg taagaaaata
     4321 ggctcgattc aacttatgtc cggtggcgaa aaagcgttaa ccgccgtagc attgatcttt
     4381 tcgatctttc taatcaagcc aagtccgttt tgtgtgctgg acgaggttga tgccccactc
     4441 gacgaccata acgtgggtaa gttcaatatg ttgctaaagg aaatggctat tcggagccag
     4501 ttcatcatta taactcacaa taaacgtact atggagctca acgacaagct ctatggggtt
     4561 accatggagg agccgggggt ttccaaaatg gtgagtattc agttgcagta aattttgctc
     4621 atattgttaa atattcttta ctttttttgt aaatagctga aattatttga gttttttcta
     4681 aacaaaagcc ccttgtttta atctggatcg cttaaaatag aggtaggagt aacacctcta
     4741 tgtcgaagct gcgggtagga cccgcgaccg aacaatacgc gcaagtggcg agtcagcggc
     4801 tcgaaactgc tacggcaata aagcaagggc cacccgctcc gggaaccccg cttgcggtgc
     4861 cgtgggaaac cgcccacgct caggtaaaca tggcgctcaa ttgggcacaa agcatggcac
     4921 ccactcggga cggcatctac gttgttggcc ctgtttcggt aaaaaatcca cccgtaaaac
     4981 cagtatctag tagttggggc atgcgtagtt tttggagcct agtattgttt gcaggtgcca
     5041 cagttttttc tggatgcttc ggatccaaaa ttgttccagc agggaacacc gattcggtgg
     5101 tgaatacccc ggcagacata ggtccttcaa acaatgctgt ttgtaacccg ttcggaaatg
     5161 ccaatcacgc tggcgccggt tacgcacacg gggtcgtagg gaaattacag tatctcaccg
     5221 acgatatgcc gagatacaac aaccttaatg attttatgaa taacgcccaa gcggtggatg
     5281 cgtcgattta ctttggcaag atcgacattc caactcgtcc atttgaccgc ggatttatta
     5341 ccttggacgg tgaagctatt ttaaacagca gtggaactac cttgtatgag tacttttctc
     5401 tacgacttga atctatgctg cgattacaag atgcctcggc cccaaaaaaa tatcagttcg
     5461 ggcttttggc agacgacgga gcgattctta gcgcagattt tggtagcggt tatgagacga
     5521 taattaacaa tgacggaacc cacccctcga agctaatgtg ctcgaccagt ccggttgagt
     5581 tggattttac gagcgctctg ccaatacgga tcgactatta tcaggggcct cgataccaca
     5641 tagcgctggt gctcgtttgg cgtgaatggt cggacgatgc ggctttcgat ccaaacgatc
     5701 cgttgtgcgg caagcaaggc aatggtcact ttttcaaatt tgcagaaaat ccgccgtcac
     5761 cgcagccggc ctgggtaaat ttactggctc gtggctggtc ggtagtgcct gccgaagtat
     5821 ttgagcttcc ttctaatatc gcatcaaacc cttgtgatgc agtcgagtcg atgaccacca
     5881 cacttacttc aattagcccg aattcagaat ttactcgttc gccagatatt acatttgaat
     5941 ttgaatcgaa tcgatcgggt gcattgtttc agtgtagtat cgatggcggc gactttagta
     6001 gttgtacgag cccaaaaacc tacattaacg ttggcaccgg ggcgcacact tttacagttc
     6061 gcgccattga tgacatcggt aactttgatg aaattggggc atcacatagc tggacggtcg
     6121 atcaatccgt tccaacgatc gtagtaaccg cttcgtcggt cactagtaat tcgatcaccg
     6181 ttacttggac taccaacgaa cccgccacaa ccagcttggc ctggggactc ggcggagaca
     6241 caagtcaaat tattcctgac gacggagttt acgataccaa tcatagcgta acgcttaccg
     6301 gacttaccgc ggatacagtt tattcctatc gaatgatcag tactgatcaa gctggcaaca
     6361 ctatgacttc gtcacgtcgc gtggttcgta cgagcccgta atcgaaaata agcggccgtc
     6421 agaaaaagta aaataaaaat ccatccggac tcgccatttt gaatagttcc gcacccggcg
     6481 gtaagcttca ggtcgacttt cgcgagaccg gagtccgcga agtcgatcac gattcgagac
     6541 acctctggag acgctccagc aatagcagca gtggtgatat taattttgaa ttttaagtaa
     6601 cgataattct taccactcag tttagtgata tcgctttcgt aatccgaaaa taccgatcca
     6661 tcaaccgatc cagcgtaaga caggctaatg gtgccactat tgagagtttg ggttactgtc
     6721 ggggcggttt taaagttcgc gtttagagtg ccgaggtcat aagccgcact ttgtatgacg
     6781 tagctccgag aagggaacac gtcatcggtg tcgtagtccg ctacgtttga atgggctgga
     6841 cgatttcccg gcgaagaatc caatcgaatt tttcccactg ttccgtcgct tgcggcgccg
     6901 ccgcaggcgc tggttccacc tgtcccgtta gtcacagatg gtgcaggaat agcgagaggg
     6961 atgtccgcca gagtttgtag ccatactgcg cctccgtggc cgccggcgcc cgcaccggaa
     7021 cagttggcac cgtcagaggt aattccgtta ccgccgttgc cgccttctgc ttcaatagtt
     7081 ccgacagtca gcgaaccaac agctgtgata cgaacggtgc ctcctcctgc gccacccgcc
     7141 ccgcccgtcg cgtattgaaa gccgaccgta tttcgatgcc cgccgccacc gccgccgccg
     7201 ccaccgcata tgaacgcggt ggcgttgtcg aagttggctc ctggcaaagc tgaagtcgct
     7261 ccaccatcgt ctccgttggt cccgtttact tggccaagac caccggaggc gtcggtagcg
     7321 ccggtgtaga taacaccgtc ggccccgttt cctactgcac ctgcgcttgc ggttccaccc
     7381 gcgcccccta ctgctggaca cgtgacagcg gtgccgccgc tgggtgcggt actcgagcca
     7441 tttgccacgc cgtcacttcc tgccgttcca tttacttgaa tcgtaggtgt aatgttgacg
     7501 gtggttaggg agcgaatgat caacggatta cttccagtaa ccgtgatggt gccgccggtg
     7561 atgtttactc gcttaaagtt gtaaccgttg ggtttagcat cagtgtcgaa actgtagccg
     7621 cccgaggtat cgagtatccc gtcgctgccg tcaccaaaac taattggtcg gtgagtacca
     7681 cctccggcgt cggctactcg cccagcttcg gctttcgagg taacagtgtt ccatagtccc
     7741 gtggtgttgg tgagatctgc accatcgagt gacgtaaagt cctctaccag ttgcccgccc
     7801 gatatcgagt aagcatgagt tctaaccgcg agtacgcaaa ttaccaaaac gaagactcta
     7861 gtaatagtaa ccaattttaa ttgctcctcc gtaagaaatt tctttggtgg atattcgccc
     7921 caaaagttca gcttccgaaa aaaaatgtga actcaaatga gcgttcgtgt tcagcgggcc
     7981 gaagcgcatt tttgctttgg cggaaatgtc agcgccaatt ccagtcattg cgaaaggtgc
     8041 gatcgaagca gaaaaagtac tacgaagagg ccttttttga gactgtccga ttccaagtac
     8101 agcccccaaa agaatcttgc gttcactttt tagacttcta cctttccagc tcaactcgtg
     8161 ggtgtttgag gcgtagagtc cataggtcag agctcgttcc gaactaatgt cgcgttcttg
     8221 tcccaaggta attctggcaa gtggcaatgc taccttttcg acaagtacgt tcttattcgt
     8281 tggctctcct tgttcggcaa tgatcgaaag acccatgaac caatccgtgg agtcgttttt
     8341 tcgtttccta acagtcagat caaccagcgc tggaacaaag ccggaggcag tagcggtttc
     8401 tggattggag tcgctattta gttttcgtga atggtagata gaaccgaccg ctattcctat
     8461 tcgaatgcga tttaaacggg ctagagtcgg gtcttgataa ggttcgatca aaatatgttt
     8521 ggtgggacta aattcgccct ctttgcctcc tctatccacg gcgagtacgc gaaagtacat
     8581 cgatcgttct ttgggagatt gtctggtttg taatataaac cgagttttta cagtctggtg
     8641 ttttagtata
//
LOCUS       DAOMXO010000014         8373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_4447_length_8373_cov_4.978000, whole genome shotgun sequence.
ACCESSION   DAOMXO010000014 DAOMXO010000000
VERSION     DAOMXO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4447_length_8373_cov_4.978000"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLH57_00630"
     CDS             complement(<1..442)
                     /locus_tag="PLH57_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01142.1"
                     /translation="MSRSVRVLDRVLFGLYAFVGLLVLTGGYYHRSEQFHFELAQIDP
                     FAWTILVLWVLRFQMSRKWLSSNESKFANATLQFIQRQPITLLSVGIGLYIAFHFWIS
                     SWRYDSFQASAYDLSYVLQSIAGTDRHGFLRASIATGGSYLGEHF"
     gene            complement(427..3111)
                     /gene="secA"
                     /locus_tag="PLH57_00635"
     CDS             complement(427..3111)
                     /gene="secA"
                     /locus_tag="PLH57_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRK01143.1"
                     /translation="MLNVIAKKLFGTQNERELALITPLVSKINSLEAATKALSNDQLK
                     AKTAEFKERVSRGETLDALLPEAFAVVREAGWRVLGQRHYDVQMIGGTVLHRGKIAEM
                     RTGEGKTLVSTLPSYLNALSGKGVHIVTVNDYLARRDSEWMGRLHNFLGLTVGVVVHG
                     VGDLERKKNYRCDITYGTNSEFGFDYLRDNMKFSLDDYVQRELNYAIVDEVDSIFIDE
                     ARTPLIISGPTDDSTDKYYAINRIIPKLDKSDYTVEEKSKSVALTDSGVEKIEKYLGV
                     NNLYEPHNIELVHHVNQALKAHVIFKRDVDYVVKDGEIIIVDEFTGRLMAGRRWSDGL
                     HQAVEAKEDVEIENENQTLATITYQNYFRMYSKLSGMTGTADTEAAEFKKIYNLDVVV
                     VPTNRPMVRKDHADVIYKSIQSKYNAVVNELKTLHEKGQPVLVGTISIEKSELLSSLL
                     KRAGVPHNVLNAKQHEREAEIVAQAGRRKMVTISTNMAGRGTDILLGGNPEFLAKQKV
                     DPAASPEEYAKVLEQFKVQCAKEREEVMALGGLFILGTERHESRRIDNQLRGRAGRQG
                     DPGESRFYLSLEDDLLRIFGSVNIGKYMEEDVPIEHRWITKAIENAQKKVEAHNFDIR
                     KHLLEYDDVMNQQRKVVYAMRREVLAGHDLKPRVLGMIDEIADAVAQDFFPDRRKGSP
                     AWDLAALNESATLTFRAPVSVGETDLPRILSAGDLSALIVKQVEAAYDEKEKQLGVAH
                     DGSGQPVMRNLEKMVTLTTLDHLWKDHLLAMDHLREGINLRGYGQKDPLLEYKKEGYN
                     YFQMMYGQISYDVVRKLFEIQVQREESLEEMAPKPQPMVFSHGGEPENAPRPQGSAPR
                     AQATSATPRATTPDGDKIGRNDPCPCGSGKKYKKCHGA"
     gene            complement(3182..4387)
                     /locus_tag="PLH57_00640"
     CDS             complement(3182..4387)
                     /locus_tag="PLH57_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01144.1"
                     /translation="MIRFFTNFLFGVLLLSHSALGAVNIQVVDSRNGSGCGNVLRGLA
                     PVDSEVIAELNRSKIPRLHVFDILYSLGGTPIELLAMFNGVPVIESDTVRGEASPNSI
                     YRIRVAATPERLRKLLEEAGSGVRWVGFEWNSSVVTRALGHQAALARVEQQSVSPHLN
                     SVITINVTIAGDAPIRARVAQRIAHLPVILVDKSASAIALPKREGLVISKRANALLSE
                     SGSDFVVLRAQVVSSKESPLSRDWLQGFGTATVTYIVESREHEKHPNTIDGTEWVLFS
                     GSRRETQEFLKDPLVRYVELQGEIRASDGFWNKGKLQRKTTAVSVSLGSTERIASTAF
                     YEQAASLGLKIHNRGQQSSYAGVEWTDIIITGPAASIQELVMGLDSSSSVREISAKQL
                     AEIPRDPHY"
     gene            complement(4465..5676)
                     /locus_tag="PLH57_00645"
     CDS             complement(4465..5676)
                     /locus_tag="PLH57_00645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRK01145.1"
                     /translation="MAANKFFTIMVVPEKTQKVRKIIIPHYVFHGMIIAIAFAAVLAV
                     VMVLDYANVMNQISENKKLKVENRQLRQSVQVFKNKMFTIENTLDRVKTFATKLRIIT
                     NIEDSPMSPSIPESMPTLPSQPALPPFAPSAPHSQAPGPAPPNVTQRIPTRPGNHLGS
                     PIEADTDLEDTLNPLADKIIDPMYAQESVPRYDDRRSERKNLLASLMSGAQEITMALS
                     DTEASLYVQSEFEKLDLAYDAVNSFALETEQETQFILEKLGEKRTLLASTPTRLPTLG
                     YVTSEYGVRISPIDGRRKMHEGIDIANRYGADIIAPADGLVVFSGVKPAYGKLVILDH
                     GNGIETRFAHNSKTTVKVGDRVKRGQRIANVGSTGHSTGPHCHYEVHAGGMPVDPCWY
                     ILDHPAICRSR"
     gene            complement(5686..6450)
                     /locus_tag="PLH57_00650"
     CDS             complement(5686..6450)
                     /locus_tag="PLH57_00650"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRK01146.1"
                     /translation="MEPPAMMQLKAAGLSHVGMKRNQNQDSFMLAPELGLFIVADGMG
                     GHKGGQIASLMAVTTIIDFLRDHVSKNGGIADARATLTQALEHANQAIQKKGQTEVDL
                     AGMGTTATALLFHGGKLFIAHVGDSRSYLVHPNRIWQITRDHSLVQEKLRAGLITREQ
                     MKSDQNKNVITRSVGFEPEVDVDLYEIDPHPGDVFVVCSDGLSGMISDSIIREVVEKR
                     VFSQNSVEKAAKELIEKANLQGGDDNVTAVVIQLHE"
     gene            complement(6497..7858)
                     /locus_tag="PLH57_00655"
     CDS             complement(6497..7858)
                     /locus_tag="PLH57_00655"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRK01147.1"
                     /translation="MQTLNMAFDKLHQFKTKGTIDAFEILFQRSSQLGIEVQGGKVDH
                     LSRATDQGMAFRVLKDQRMGFSYTFDLSENAVNVALRTAIDIAALMPQDPLLSLVTFR
                     DQKYTTIDSKSGHDVYDLQGIERNIEEKIELAREVERAARSVDQRITRVRKAGYDESI
                     GEIVIVDTDGTRLSHHGTTFSTEILCVAEDGQSAETGYESISSPLSKNLKATWVGEMG
                     GRNALELLHATPAPTRKCPAIFRNSVVAELIGFLSGSFSAENIDKNFSLLSLNKIGQQ
                     VFSKELNIYDDGLLPGGLATGAFDGEGMPQSRTTLLKAGVVESILADQYYARKMKIKA
                     TGNCSRGIKTPPSIGTTNLYLEKGPHSFEALLKQAHNGVVITEVMGLHTANPVTGEFS
                     VGASGLLIENGQITKPVKGFAIAGNLTDLLSKVTAVGNDHRFWGTTGASSFMVSELNI
                     GGS"
     gene            7931..>8373
                     /locus_tag="PLH57_00660"
     CDS             7931..>8373
                     /locus_tag="PLH57_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HRK01148.1"
                     /translation="MAREFDIVSLVGKHQSTENYKEFHWEGSFQEYVDLVKKNPLITR
                     NSFQRMYDMILSYGVESYVDFKKNVARYKFFTDPIDNGKDAIYGLDIPLMKLVHFFKS
                     AAYNYGTEKRVLLLHGPVGSSKSTIARLLKKGLERYSRTDEGKLYT"
BASE COUNT         1947 a         2017 c         2230 g         2179 t
ORIGIN      
        1 aaaaatgttc gcccagatac gatccaccag ttgcgattga ggcccgcaaa aaaccgtggc
       61 gatcagtgcc cgcaatactt tgcaggacgt aacttaaatc gtaggcgctc gcttgaaacg
      121 aatcataacg ccacgacgat atccaaaaat gaaaggcaat gtagagacca ataccaactg
      181 aaagtaaggt aatgggctga cgctgaataa attgtagggt ggcgttagca aacttagatt
      241 cattgctcga gagccacttg cgcgacattt gaaaacgcaa aacccacaga accagaatgg
      301 tccaagcaaa agggtcgatt tgggctaact caaaatgaaa ttgctcggag cggtggtagt
      361 aacccccggt gagcacaagc aggccgacga acgcgtacaa cccaaacaaa actcggtcta
      421 agactcttac gctccgtgac actttttata tttcttgccg ctaccgcaag ggcacgggtc
      481 gttgcgacca atcttgtcac cgtcgggtgt ggtcgcacgt ggagtcgctg aagtcgcctg
      541 cgcacgagga gccgacccct ggggccgtgg ggcgttttcg ggttcgccgc cgtggctaaa
      601 aaccatgggc tgtggcttcg gagccatttc ttctagactt tcttcacgct gaacttgaat
      661 ttcaaaaagt ttacgtacga cgtcgtagga aatctggccg tacatcattt gaaaatagtt
      721 ataaccttct tttttgtact ctagcagcgg gtctttttgc ccgtaaccgc gcaagttaat
      781 gccctcgcgc aagtggtcca tggcaagaag atgatctttc cacaaatgat ccagcgtagt
      841 gagagtgacc attttttcga gattgcgcat taccggttga ccagaaccgt cgtgggcaac
      901 gcctagttgt ttttcttttt cgtcgtaggc cgcttcaact tgttttacga tcagcgctga
      961 aagatcacct gcgctgagaa tgcgcggcaa atcggtttcg ccaacggaaa ccggggctcg
     1021 aaacgtgagt gtggcggatt cattaagcgc agccaagtcc caagccggag aaccttttcg
     1081 gcggtccgga aaaaaatctt gtgccactgc atcggcgatc tcatcaatca tgccgagcac
     1141 tcgtggtttt aaatcgtgac ccgccagtac ttcgcggcgc atggcgtaga ccaccttgcg
     1201 ctgttgattc atgacatcgt cgtattcgag taagtgtttt cgaatgtcaa agttgtgagc
     1261 ttccactttt ttctgagcgt tctcgatggc cttggtaatc caccggtgtt cgatgggtac
     1321 atcttcttcc atgtacttgc cgatgtttac tgaaccaaaa atacgaagca gatcgtcttc
     1381 gagcgaaaga taaaaacgtg attcgcccgg gtctccttgg cggccagcgc ggcctcgcaa
     1441 ctggttgtcg atgcggcggc tttcgtgacg ttcggttcct aaaataaaaa gtccgccaag
     1501 cgccatgact tcttcgcgtt ctttagcgca ctgaacttta aactgctcga gcacttttgc
     1561 gtattcctca gggctcgccg ctggatcgac tttttgttta gctaaaaact cggggttgcc
     1621 acctaataaa atgtctgtgc ctcgacccgc catgttggtg gagatagtca ccattttccg
     1681 gcggcccgct tgggctacga tctcggcttc acgctcgtgt tgtttggcgt taaggacgtt
     1741 gtgaggaaca ccggcgcgtt tgagcaacga agacagtagt tccgatttct caatggaaat
     1801 cgtaccgacc aacacaggct ggcccttctc gtgcagtgtt ttgagttcat tgaccactgc
     1861 attgtatttg ctctggatag acttatagat cacatcagcg tgatctttac gaaccatcgg
     1921 ccgattcgtt ggtacgacga ccacgtcgag gttatagatt tttttgaatt ccgctgcttc
     1981 ggtgtcggca gtacccgtca tgcccgagag tttggagtac atgcggaaat agttttggta
     2041 ggtgatcgtc gcgagagttt gattttcgtt ttcgatctcg acgtcttctt ttgcctcaac
     2101 cgcttggtgc aacccatcgc tccagcgacg acccgccatg agacggccgg tgaattcatc
     2161 gacaataata atttcaccgt ctttgaccac atagtcgacg tcgcgtttga aaatgacgtg
     2221 tgctttaagg gcttgattca cgtggtgcac gagctcaatg ttgtgaggtt cgtacaggtt
     2281 gttcacaccc aagtattttt cgattttttc tacacccgag tctgtgagcg caacactctt
     2341 tgatttttct tcaaccgtgt aatcactttt gtcgagtttg ggaataatgc ggttgatggc
     2401 gtaatacttg tcggtcgagt cgtcggtcgg accactgata atgagcggcg ttcgggcttc
     2461 gtcgataaat atggaatcca cttcgtcgac gatcgcataa ttgagttctc gttgaacgta
     2521 gtcgtctagc gaaaacttca tgttgtcgcg caggtaatcg aagccgaact cagagttggt
     2581 gccgtaagta atgtcgcagc gataattctt tttgcgttcc agatcgccta cgccatgcac
     2641 gaccactccg actgtgaggc ctaagaaatt atgcagacgc cccatccatt cggagtcgcg
     2701 gcgcgcgaga tagtcgttca ccgttacgat gtgcacgccc ttgcctgaga gcgcgttgag
     2761 ataagacggc aacgttgaaa ccagggtttt accttcaccc gttcgcattt cggcaatctt
     2821 gcctcgatgt agaactgtgc cgccaatcat ttgaacgtcg taatggcgtt ggcctaagac
     2881 gcgccaacca gcttcgcgaa ctaccgcgaa tgcctcgggt aagagggcgt cgagtgtttc
     2941 accacgagaa acacgctctt taaattctgc ggtttttgcc ttgagctggt cgtttgagag
     3001 cgctttggta gcggcttcga ggctattgat cttcgagacg agcggagtga tgagcgcgag
     3061 ttcgcgttcg ttttgggtgc cgaataactt tttcgcaatg acgtttaaca tgccgtatat
     3121 aaaaggggta acacacccat ttagagacga caagaaaacg ctttagcgac aatacggaac
     3181 gttaatagtg ggggtcccgt gggatttctg ccagttgctt agcggaaatc tcgcgaaccg
     3241 aggagctact atcgagcccc atgacgagtt cctgaattga ggcggccgga ccagtaatga
     3301 tgatgtcggt ccattccact ccggcgtagg agctctgttg gcctctgttg tggattttga
     3361 gtcccagcga cgccgcctgt tcataaaacg cggtcgaagc gatgcgttca gtggagccaa
     3421 ggctcactga aacggcagtg gtctttcgtt gaagtttgcc tttattccaa aaaccatcac
     3481 tggcgcgaat ttcaccctga agttctacgt agcgaacgag cgggtctttt aaaaattctt
     3541 gggtctcgcg gcgagaaccg ctaaagagca cccactcagt accgtcgatc gtattggggt
     3601 gtttttcgtg ttctcgagac tcaacaatat aagtaacagt agctgttcca aaaccttgga
     3661 gccagtcgcg cgatagaggg gactctttag aagacacaac ctgagcgcgc aacacgacga
     3721 agtcactacc actctcggag agcagagcat tggcccgctt cgagataacc agcccctcac
     3781 gcttaggcag cgctatggcg gaggcggatt tgtcgactag aatgaccgga aggtgagcga
     3841 tgcgttgagc gacgcgtgct cgaatggggg cgtcccccgc aatggtcacg ttgatcgtga
     3901 tcactgagtt taaatgaggg cttacgcttt gctgttcaac tcttgccaat gcggcctggt
     3961 ggcccagcgc tcgggtgaca acgcttgaat tccattcaaa acccacccag cgcacaccgc
     4021 tcccggcttc ttcgagcagt ttgcgtagtc gttctggcgt agcggctact cgaattcgat
     4081 aaatagaatt tggcgacgct tcgccgcgca cggtgtcact ctcgatcacc ggtacaccgt
     4141 tgaacatggc cagcaattca ataggagttc cgcccagact ataaaggata tcaaaaacgt
     4201 gaaggcgcgg gattttggag cggttgagtt ctgcgatcac ttcgctatct accggggcaa
     4261 ggcctcgtag cacattgccg caaccacttc cgttgcgcga gtctacgacc tgaatattca
     4321 cggcaccgag cgccgaatgg gaaagtagca ggactccgaa caggaaattc gtaaaaaatc
     4381 gaatcatgaa accacccctt ttgacgcgat gggtatataa tatttaatga atttgatcaa
     4441 gtttgggtgg aattcagaga atagttaacg cgaccggcaa atcgccgggt ggtcgagaat
     4501 gtaccagcag gggtcaaccg gcatgccgcc ggcgtgaact tcatagtggc agtggggccc
     4561 ggtggaatgg ccggtgctgc cgacgttggc gatgcgctga cctcgtttga cgcggtcacc
     4621 aactttgact gtggttttcg agttgtgagc aaaccgcgtt tcgattccgt tgccgtgatc
     4681 taaaatgacg agtttaccgt aggccggttt aactcctgaa aaaaccacta gaccgtcggc
     4741 aggggcaatg atgtcggctc cgtagcgatt ggcaatgtcg attccttcgt gcattttgcg
     4801 gcggccgtcg atgggcgaga tgcgaactcc gtactccgag gttacgtagc cgagcgtggg
     4861 caggcgagtg ggagtgctgg caagtaaggt tcgtttttcg ccgagctttt ctaaaataaa
     4921 ctgagtctct tgttcggttt cgagagcaaa cgaattgacg gcatcgtagg ccagatcgag
     4981 tttctcgaac tcgctttgaa cataaaggct agcctcagtg tcactcaaag ccatggtgat
     5041 ttcttgagcc cccgacatta atgaggctaa aagatttttt cgctcagatc ggcgatcatc
     5101 gtagcgtggg accgattctt gcgcgtacat gggatcgata attttgtcgg ccagtggatt
     5161 gagagtgtct tcgagatcgg tgtctgcttc gatgggagaa cctaagtggt tccccgggcg
     5221 ggtaggaatg cgttgagtca cattgggagg tgcaggaccc ggtgcctgac tgtgaggagc
     5281 tgacggggca aacggtggca acgcgggttg cgacggaagc gtcggcatcg actccggaat
     5341 gcttggcgac atcggcgagt cttcgatgtt ggtaatgatt cgaagtttgg tggcaaacgt
     5401 ttttacgcgg tccagagtgt tttcgatagt gaacattttg tttttaaaaa cttgaaccga
     5461 ttgccgaagt tggcggtttt cgactttaag ttttttattt tcgctaattt gattcatgac
     5521 gttggcgtag tcgagcacca tgacaacggc caagaccgcc gcaaatgcga tcgcgatgat
     5581 cattccgtga aagacatagt gcggaataat gatcttgcga actttctgag ttttttcggg
     5641 caccaccatg atggtaaaaa atttattcgc tgccatcgtt ttgttttact cgtgtaattg
     5701 aatgacgacg gcggtaacat tgtcatcgcc gccttgtagg tttgcttttt cgatcagctc
     5761 tttggcggct ttttccaccg agttttggct gaaaacacgt ttttcgacca cttcgcgaat
     5821 aattgagtcc gaaatcatgc cgctcagtcc gtcgctacat acgacgaaaa catcacccgg
     5881 gtgggggtcg atttcgtaga gatcgacatc gacctcgggc tcgaagccta ctgatcgcgt
     5941 aataacgttt ttgttttggt cgctcttcat ttgttcgcgg gtaattaagc ccgcgcgcaa
     6001 tttttcttgc acaagtgagt ggtcgcgcgt gatttgccaa atgcgatttg ggtgcaccaa
     6061 ataacttctg ctgtcgccca cgtgtgcgat aaaaagcttt ccaccgtgaa aaagtagggc
     6121 ggtggctgtg gtgcccattc cggctagatc cacttcggtc tgcccctttt tttgaatggc
     6181 ttggtttgcg tgctcgagcg cttgagtgag cgtcgcccgg gcatcggcga tgccgccgtt
     6241 tttagaaacg tggtcgcgca aaaagtcgat aatagtggtg accgccatga gtgaggcaat
     6301 ttggccaccc ttgtggcccc ccatgccgtc ggcaacaata aaaagtccga gctcgggggc
     6361 gagcatgaag ctgtcctggt tttggttgcg cttcattccg acgtgagaaa gtcctgctgc
     6421 tttgagttgc atcatagctg gtggttccat agtaaaccgc gaagtcggag gcgtaaactt
     6481 taaacattgt gaaggtttag gatccgccaa tgtttagctc agacaccata aaactagacg
     6541 ctcccgtggt gccccaaaaa cggtgatcgt tacctactgc agttactttt gataaaaggt
     6601 ctgtcaagtt gccagcaatg gcgaacccct tcaccggttt ggtgatttgg ccgttttcta
     6661 taagaaggcc actagcaccc accgaaaact caccggttac cggatttgcg gtgtgaaggc
     6721 ccatgacttc ggtgatcacg acgccgttgt gcgcctgttt aagaagcgct tcgaaactat
     6781 gaggaccttt ttccaaatac aaattcgtgg ttccgatcga aggaggtgtt ttaatgccac
     6841 gagaacaatt gccggtggcc ttgattttca ttttgcgagc gtaatactga tccgccaaaa
     6901 tggattctac gacccctgcc ttcaacagag tggtgcgtga ctgcggcatg ccctctccgt
     6961 cgaacgcacc ggtggctagg cccccgggta atagtccgtc atcatatata tttagttctt
     7021 ttgaaaaaac ttgttggccg attttattga gcgataagag cgaaaaattc ttgtcgatat
     7081 tttctgcgga aaaagaacca gagagaaaac caatgagctc ggccaccacc gaattgcgaa
     7141 atattgccgg acactttcga gtgggggccg gagtggcatg cagtagctcg agcgcgttgc
     7201 ggccacccat ttcgccaacc caggtggcct tgaggttttt gctgagtggg gaggaaatcg
     7261 attcatagcc ggtttccgca gattggccgt cttccgccac gcagagtatt tcggtcgaaa
     7321 aagttgtacc gtgatggctt aagcgtgttc catcagtgtc gacaatgacg atttcaccga
     7381 ttgattcatc gtacccagct tttcgtaccc gcgtgatgcg ttgatcgacc gatcgcgcgg
     7441 cgcgttcaac ttctcgggcg agttcaattt tttcttcaat attacgttcg atcccctgga
     7501 gatcatagac atcgtggccg ctttttgagt cgatcgtggt gtatttttga tcgcgaaagg
     7561 tgacgagact gaggagcggg tcctggggca tgagagctgc gatatcgatg gccgtgcgta
     7621 gtgcgacgtt cacggcgttt tcacttaagt cgaaggtgta ggaaaacccc attcgttggt
     7681 cttttagtac gcgaaacgcc atgccctggt cggttgcgcg ggaaagatga tctacttttc
     7741 caccctgcac ttcaatgccg agttgcgagc tgcgttgaaa aagaatctca aacgcgtcaa
     7801 tagtgccttt ggttttaaat tggtggagtt tatcgaacgc catattcaga gtttgcatat
     7861 gcaaggcagc gtaactttac attcgcactg atgtaaagat agaatggagt cgggatcata
     7921 gggggacacc atggcacgtg aattcgacat cgtgagttta gtcggcaaac atcaatcgac
     7981 tgaaaactac aaagagtttc actgggaagg ttcatttcaa gagtacgtag atctcgtgaa
     8041 aaagaatccg ctcatcacgc gcaattcgtt tcaacgaatg tacgacatga ttttatcgta
     8101 tggcgttgaa agttacgtcg atttcaaaaa gaacgtcgcg cggtataagt tcttcacgga
     8161 ccctatcgat aacggaaaag acgccatata cggcctcgat attccgctca tgaagcttgt
     8221 tcattttttt aagagcgcgg cttacaacta cggcaccgaa aaacgagtgc tgctgttgca
     8281 cgggccggtg ggcagttcga agtccaccat tgcgcgccta ttgaaaaaag gattggagcg
     8341 ctattcgcgc accgacgagg gtaaactata cac
//
LOCUS       DAOMXO010000015         8365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_4453_length_8365_cov_4.414681, whole genome shotgun sequence.
ACCESSION   DAOMXO010000015 DAOMXO010000000
VERSION     DAOMXO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4453_length_8365_cov_4.414681"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLH57_00665"
     CDS             complement(<1..206)
                     /locus_tag="PLH57_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01149.1"
                     /translation="MSRRRINTTAFLLVLTFAIHATLVGGCASKKTRDEDLDTGNAVE
                     NSYDVAAPPTVGPQPDEDYTPPEES"
     gene            complement(223..984)
                     /locus_tag="PLH57_00670"
     CDS             complement(223..984)
                     /locus_tag="PLH57_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRK01150.1"
                     /translation="MAKSEVKKTTRLDVLVVEQGLADSREKAQALILAGKILVGDHLY
                     TKPGIKIESSLKLRVRGEVDHYVSRGAHKLLGALDHFGIKLEGALCLDIGASTGGFTQ
                     VCLERGAAKVYAVDVGHNQLDWKIRSDKRVVNFEKVNFREAHITHGTLIPKNMDFICI
                     DTSFISLKLILPAAKEFLKKGGVLVALIKPQHEVGKENVGKGGIVQDQAIREAARDGI
                     SSFAQSIGFTQKGLIESPIKGTTGNTEYLIHLVLN"
     gene            complement(971..1228)
                     /gene="xseB"
                     /locus_tag="PLH57_00675"
     CDS             complement(971..1228)
                     /gene="xseB"
                     /locus_tag="PLH57_00675"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRK01151.1"
                     /translation="MQKKSEKKDGLEGSFTRLNEIVNRLEQNDCSLEESLKLYEEGVK
                     LAGAAHRVLNEAERKIEILSKSAETGFPISEPFGMAKDGEV"
     gene            1283..2326
                     /locus_tag="PLH57_00680"
     CDS             1283..2326
                     /locus_tag="PLH57_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRK01152.1"
                     /translation="MASILYLFLVVSLLISGGKLAFAKSEAFESGVLETEAEHFSDST
                     PHPKENAHFYDLTPDARDALLADKSNRISDLFSVPRGIRHMTGFWLEIYAKYSSLQTV
                     LYDREHPNFVYDVVDLRDLFKKGSSPVVIELTGKKRIQNKLNEIRSAIDQISKNSSVR
                     FKPGSAGAKLVRYWGRKPKIGWAKVRENLRTQTGQRDRVIQGITHAERFIPAMEAIFK
                     DFNLPVELTRLPLVESSFNLKAVSKADAVGVWQFLERSALEYMKVDEKNKIDERLSPI
                     KSTYGAAKMFVRNYRLLGDYALAIIAYNHGQRNLIKLRHKYRTENISALLRSENSPLG
                     YASRNYMQSFWRC"
     gene            2320..2628
                     /locus_tag="PLH57_00685"
     CDS             2320..2628
                     /locus_tag="PLH57_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01153.1"
                     /translation="MLIAEEYRDELFGLPLTREVEHVSIVKLKAPSSMFDVAAHYRLS
                     LQEIKIFNPDIFDLKRTLPVGTRVVIPRKVDQAFVEKEKSRSPSSEAPQIAGEVINLY
                     "
     gene            complement(2632..5016)
                     /locus_tag="PLH57_00690"
     CDS             complement(2632..5016)
                     /locus_tag="PLH57_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRK01154.1"
                     /translation="MYLKKAAGVSHHVFKGATYALSACGLICAAAVTVAAVQALRIDA
                     KTVKIGRNLVLSETAVKIADIGDSRFADSYIPKIEAAPVLVELNAANISKWQRTAPSY
                     RFSKEVFENAPDTQAAVEISGIQKNEISDNNLNDLVIVVQEYEPEEDFDRSGLMEVLT
                     SKEPTAPTEKLLRPIAKIKRPEVKIGKKIPFGKVSIQNPKGSNALNSQTRKPTVKTVA
                     IQSAVPLIAKNDPEKIQDLTTESKLEYSQLVAMFSDDGRQTLSDADPIPVQPIQLNEI
                     QAQIDPHSYPDALLALAKPTETNTDVDTQRELKAATEVEKPASVPVIQPIPILDSQLS
                     SQPLHEGRVVVTLPSAKPAPTAAPDLSKEEDTRTKEEDLTELASASSQLTARKVEPAT
                     IQGQQFQLRVVDAFDTARTTLSSVKAQVLGTAVFGESFSDGTIVFSDVTIQGVLPIVL
                     TKEGYLGRKIDLKQNYNEIELVSENSARMISEILGQPFNTDQGVVAGIVTDTESNPVA
                     GIQVALSSVSSYSNNAPAELKANYFSDAGVPNREYIVTSANGRFVFLNVPSGTYLMTM
                     RGLDNREFASRLFHVSSNEGIMRKFSIGQLKTVRGRVINAAANQPVAGAKIRLLGQPM
                     ESEVESKRTGGFAIESVYVDCSELNYLQVAKNGYYHNRIDFNCDAFDRPKNVYVLPAA
                     SIDGDALSADVSLNEHTGVVVGHISSKSSSKVQLWGPDEMNPQQTLRGRDYYYGRDGV
                     LRPDLDKTTTSGRFTIFDAPIGLSYLQTLSPDGEYTLSYWPIMISPSTVNVYLQ"
     gene            complement(5104..5520)
                     /gene="atpC"
                     /locus_tag="PLH57_00695"
     CDS             complement(5104..5520)
                     /gene="atpC"
                     /locus_tag="PLH57_00695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRK01155.1"
                     /translation="MLSLTIVTPERRVVGPVSVKSLTAPAGKGEVTILPGHARLLTTL
                     ETGVVSFERENGEKQFAAVSFGFVEVHGDKIVMLAETLELAQEIDVDRARRALEKAQE
                     KLKAKENFELDMVKWQRKTQRSQIRLQAANYLLPPH"
     gene            complement(5543..6964)
                     /gene="atpD"
                     /locus_tag="PLH57_00700"
     CDS             complement(5543..6964)
                     /gene="atpD"
                     /locus_tag="PLH57_00700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRK01156.1"
                     /translation="MSATSQHSVGKIKQVIGPIVDVNFESGRLPAIFNAVRVSNPALS
                     DKAGNLVLEVAQHLGENTVRCVAMDSTDGLVRGMDATDTGAQISVPVGRETLGRILNV
                     IGKPVDEAGPVTAKKYYPIHRSAPAFRDQSVTVQPFFTGIKVVDLLAPYARGGKIGLF
                     GGAGVGKTVLIMELINNIATHHGGYSVFAGVGERTREGNDLWMEMKESGVINKTSLVY
                     GQMNEPPGARARVALTALSIAEYFRDEENQDVLMFVDNMFRFTQAGSEVSALLGRIPS
                     AVGYQPTLATEMGELQERITSTNKGSITSIQAVYVPADDYTDPAPATTFAHLDATTNL
                     SRQIAELGIYPAVDPLASTSRILDPNVVGEVHYATARRVQSVLQRYKDLQDIIAILGM
                     DELSEEDKLVVSRARKIQRFLSQPFHVAEQFTGIKGKFVKIEDTIRGFNEICDGKHDD
                     LPEQAFYLVGTIEDAIEKAKTLN"
     gene            complement(6985..7857)
                     /gene="atpG"
                     /locus_tag="PLH57_00705"
     CDS             complement(6985..7857)
                     /gene="atpG"
                     /locus_tag="PLH57_00705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRK01157.1"
                     /translation="MASAKDLRKRISSVKNTQQITKAMKMVSAAKLRRAQENIVSLRP
                     YSREMSKVVGSLAQAVKASGEESHPLLEAKSEIKSCLVFVVTSDRGLCGALNANVIKT
                     AARFIKNEAPKYEKCDFAFIGKKGYEYFKNRKPGKYYQDFYAGFRMPKATQLAEELIA
                     LYLSGQYDHIRFIYSEFKSAISQSVMDESFLPVGTLGESSTTESEMTLYEPTVRDILD
                     GILPKHFATQICRIVYDALASEHAARMSAMENASKNAGEMIKKITLLYNKTRQAAITK
                     ELLEITSGAEAQKA"
     gene            complement(7861..>8365)
                     /locus_tag="PLH57_00710"
     CDS             complement(7861..>8365)
                     /locus_tag="PLH57_00710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886815.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRK01158.1"
                     /translation="TDGQIYLESDLFYSGIRPAVNVGLSVSRVGGAAQIKAMKQVAGT
                     LRLDLAQYREKAAFAQFGSDLDAATQKQLNRGARLVEVLKQGVYAPMAVERQIIMIFA
                     GTNGYLDHLPLNKLGRYEEELYRFLDAKHPDVCKDIVTTGKLDDATKTKLIKALKEFE
                     TVFGTGE"
BASE COUNT         2080 a         2002 c         1848 g         2435 t
ORIGIN      
        1 gactcctccg gcggggtgta gtcttcatcg ggttgcggtc caacggtggg cggcgctgct
       61 acatcgtagc tattttcaac cgcatttccg gtatctaaat cttcgtctcg cgtttttttt
      121 gacgcacacc cacccaccaa cgtcgcatgg atcgcaaagg taaggaccag taaaaacgca
      181 gtagtgttga ttcggcgtcg cgacatttta attaacgtgt tttcagttca atactaaatg
      241 aataaggtac tccgtgtttc cggtagttcc ctttattgga gattctatca agcccttctg
      301 tgtaaaacca atgctttgtg caaacgaact aatcccatca cgcgcggcct ctcttatggc
      361 ttgatcctgt acaattccac cttttcccac attttcctta cctacttcgt gctgcggttt
      421 aattagggca accagtacgc cccctttttt taagaattcc tttgcggccg gcagaattag
      481 cttgagagat ataaaggacg tatcgatgca aataaaatcc atgttttttg gaataagcgt
      541 gccatgagtt atgtgagcct ctcgaaagtt tactttttca aaattgacca ctcttttgtc
      601 agaacgaatt ttccaatcta gctgattgtg ccctacgtct actgcataaa cctttgcagc
      661 gccgcgttcg aggcagactt gggtaaaccc accggtactt gcgccgatgt cgagacatag
      721 cgcgccctcg agttttattc caaagtggtc taacgccccc agaagtttat gcgcgccgcg
      781 agacacgtaa tgatcaacct cgccccgaac tcgtagcttt aaggaagatt cgatttttat
      841 tcctggtttt gtgtagaggt ggtctccgac taaaatttta cctgccagga taagcgcttg
      901 cgccttttca cgactatctg caaggccctg ctctacgacc aaaacatcga gcctagttgt
      961 tttttttact tcagacttcg ccatcttttg ccatgccgaa cggctcagaa atggggaatc
     1021 cggtctcggc ggatttcgaa agaatttcga tttttcgttc agcctcattc aaaactcggt
     1081 gagcagctcc tgcgagtttt acgccctctt cataaagctt tagtgactct tctaaactgc
     1141 agtcgttttg ctcaaggcga tttacgattt cattcaatcg ggtaaaagag ccctctaacc
     1201 cgtctttctt ttccgatttt ttttgcatag taggcaaaac ctaccctctt actgctgtcc
     1261 ccgacaaccg ataaaagtat gaatggcgtc tatactctat ctttttctag tggtttctct
     1321 cctaataagc ggcggtaagt tggcgttcgc aaaatcggag gcgttcgaat ctggggtact
     1381 tgaaactgag gccgagcatt ttagcgattc cacgccccat cccaaggaaa acgcgcattt
     1441 ttacgacctc accccggacg cgcgggacgc gctacttgca gacaaatcta atcggatttc
     1501 agacttgttt tcagtacctc gcggaatccg acatatgacc ggtttttggt tagagattta
     1561 cgctaagtac tctagcctac aaacggttct ctatgatcgg gagcatccta atttcgtata
     1621 tgatgtggtg gatctccgag atttgtttaa aaaggggtca tcgccggttg taatcgagct
     1681 tactggaaaa aaacgaattc agaataagtt gaatgaaatt aggtccgcca ttgatcaaat
     1741 ttcaaaaaat agctccgtac ggtttaaacc aggaagtgcc ggagccaaat tggtgcgcta
     1801 ctggggccgt aagccgaaga ttggctgggc aaaggttcga gaaaatctga gaactcaaac
     1861 cggtcagaga gatcgcgtta ttcagggcat cactcatgca gagcgtttta ttcctgcaat
     1921 ggaggcaatt ttcaaggatt tcaatctacc ggtagagctc actcgtctgc ccctcgttga
     1981 gtcatccttt aatttgaagg cggtgtcaaa ggccgatgct gtcggtgttt ggcagttttt
     2041 agagcgctcc gcgcttgaat acatgaaggt ggatgaaaaa aataaaattg atgaacgcct
     2101 gtcgccaatt aaatctacct acggagctgc aaaaatgttt gtgcgtaatt accgactctt
     2161 gggtgactat gcactagcca tcatcgcgta caatcacggg cagagaaacc ttattaaact
     2221 gcgtcacaaa tatcgaactg agaatatttc agcgcttcta cgttccgaga attcacccct
     2281 tggttatgct agtcgcaatt acatgcagag tttctggcga tgttgattgc cgaagaatat
     2341 cgggatgaac tttttggact acctctcacc agagaggtcg agcatgtttc aattgtaaaa
     2401 ctaaaagcgc catccagcat gttcgatgtg gctgcccact accggctatc gctacaagaa
     2461 attaaaatat ttaaccctga tatttttgac ctaaaaagaa ctcttccggt cggcacccgc
     2521 gtagtaattc cccgcaaggt ggatcaggcc tttgtagaaa aagaaaaatc tcgcagccct
     2581 tcttctgagg ctccgcagat agctggcgaa gtgatcaatc tgtattaatt attattgaag
     2641 gtagacgtta acggtggatg gcgaaatcat aatcggccaa tatgaaagcg tgtattctcc
     2701 atctggggaa agtgtctgta ggtaggacag accaatgggc gcatcaaaga ttgtaaatct
     2761 tccagaagta gtagttttat ctaaatccgg gcgcagaact ccgtcgcgcc cataataata
     2821 atcgcgacca cgaagtgttt gctgtggatt catttcgtct gggccccaaa gctgaacctt
     2881 ggagcttgat ttactagaaa tatggccaac gacaacacca gtgtgttcgt tgagtgaaac
     2941 atctgcgctc aaagcgtcgc cgtcgataga ggccgcaggt aaaacataaa cgtttttggg
     3001 tctatcaaac gcatcgcaat taaaatcaat ccgattatga taatagccgt ttttcgctac
     3061 ttgtaggtag tttagttcag agcaatcaac atagacggac tcgattgcaa agccacccgt
     3121 gcgctttgat tcaacttctg attccatcgg ctgccccagc aagcgaatct tagctcccgc
     3181 cacgggctga ttagcagccg cattaatgac gcgacctcgg acggtcttta gctgacctat
     3241 tgaaaatttt cgcatgatgc cttcgttgga acttacgtga aataggcgtg atgcgaattc
     3301 tcggttatcg agtccccgca tagtcattaa ataggtaccc gagggaacat ttagaaacac
     3361 aaaccgtccg ttagctgagg ttacgatgta ttccctattt ggaacgccag catcggaaaa
     3421 gtagtttgct ttcaattctg ccggagcatt attggaatag gaagacacgg aactaagagc
     3481 tacttgaatg ccggctaccg gattgctctc cgtatctgta acgatgccag ccaccacgcc
     3541 ttgatccgtg ttaaaaggtt gaccgagaat ttccgaaatc atgcgggcac tgttttcaga
     3601 gactagttca atttcgttat aattttgttt caaatctatt ttgcgaccga gatagccttc
     3661 tttagttaaa acgattggaa gaaccccttg gatggtaaca tcgctaaaaa cgatcgtacc
     3721 gtccgaaaag ctttccccaa aaacagcggt acctaacact tgggctttaa cgcttgagag
     3781 ggtcgttcgc gcggtatcga aggcatcaac tactctcagt tggaattgtt gcccttgaat
     3841 cgtagctggc tcgaccttac gcgcagttaa ctgactacta gcgctggcca attcagttaa
     3901 atcttcctct tttgttcgag tgtcttcctc tttagataaa tcaggtgcgg cagtcggggc
     3961 tggtttagcg cttggcaagg ttaccacgac tcgaccctca tgcagtggtt ggctactcaa
     4021 ttgactatcc aagatcggaa tcggttgaat aactgggact gatgctggtt tttctacttc
     4081 ggtggcagct tttaattccc tctgagtatc cacatcagtg ttggtttcag ttggtttagc
     4141 caaggccagc aacgcatctg gataggaatg tgggtcgatc tgggcttgaa tttcattcaa
     4201 ctgaatcggc tgtaccggta ttgggtctgc atcagataat gtttgacgac cgtcgtcact
     4261 aaacattgct acaagttgtg aatactcaag tttactttct gttgttaaat cctgaatttt
     4321 ttcggggtca tttttcgcaa ttaaaggaac ggcactctgg atagccactg ttttcacagt
     4381 tggctttctt gtttggctat tcaaagcatt cgagcctttt ggattttgaa tactcacttt
     4441 accgaaaggt attttttttc ctatttttac ttctggtctt ttaatcttgg cgatggggcg
     4501 caaaagtttt tcggtgggcg ctgttggttc cttgctggtt aagacttcca ttaagccaga
     4561 ccggtcaaaa tcttcttccg gttcatattc ttgaacaaca attactagat catttaaatt
     4621 gttgtccgag atttcatttt tttgaattcc agaaatttca acagctgctt gggtgtctgg
     4681 tgcgttttca aagacttctt tcgagaatcg gtagctcggc gcagtccgct gccatttcga
     4741 aatattcgcc gcatttaatt caactaaaac cggggccgct tcaattttcg gaatataact
     4801 atccgcaaat ctagagtcgc caatatcagc aattttaact gccgtttcgg aaagtactaa
     4861 attacggcca attttaacgg ttttagcatc aattcgcagg gcttgtaccg cggctacagt
     4921 tactgccgca gcacagatca atccacacgc actaagtgca taagttgcgc ctttaaatac
     4981 gtgatgtgaa acacccgcag ctttttttag atacatcttt tcatctattg tagattctct
     5041 ggtgccaaat aaaatagact acaaattttc cgcggggtac tttggtctcg gcaaaacccg
     5101 cactcagtgc ggcggaagca ggtaatttgc cgcctgcaac cgtatctgag aacgttgcgt
     5161 tttccgttgc cacttaacca tgtctagttc aaagttttcc tttgctttga gcttttcctg
     5221 ggctttttca agggcccgcc ttgctctatc gacgtcaatt tcctgagcta attcgagcgt
     5281 ttcagcgagc atcacaattt tgtcaccgtg gacttcaaca aacccgaaag acacagcggc
     5341 gaattgtttt tcaccatttt cacgctcaaa cgacacaacc cccgtttcga gagtcgtgag
     5401 gagacgggcg tgccccggca aaatagtcac ttcgcctttt cccgcaggag ccgtgaggct
     5461 ttttacggaa acggggccaa ctactctacg ttctggagtc actatcgtta aagaaagcat
     5521 cgccacatct ctccgcgttg gtttagttca acgtctttgc tttttcgatc gcgtcttcga
     5581 tggtgccaac taggtaaaaa gcttgttccg gtagatcgtc atgtttaccg tcacaaatct
     5641 cgttaaaacc tcgaatcgta tcttcgattt tcacgaattt gcctttaatt cctgtaaact
     5701 gttcagcaac gtgaaatggc tgtgacaaga accgttgaat ttttcgcgca cggctaacaa
     5761 ccaacttatc ttcttcagaa agctcgtcca taccaagaat ggcgataatg tcctgcaagt
     5821 ccttataacg ttgtagaact gattgaactc gacgagcggt tgcatagtgc acttcaccaa
     5881 ctacgttcgg atccaagatt ctcgacgtcg acgccaacgg atccaccgcc gggtaaatcc
     5941 caagctctgc aatttgtcgc gacaagtttg tagttgcatc caagtgggca aatgttgtgg
     6001 ccggcgctgg gtctgtatag tcatccgccg gaacataaac agcttgaatc gaagtaatcg
     6061 aacccttgtt ggttgatgta attcgctcct gcaattcacc catttccgta gcaagtgtag
     6121 gctggtaacc caccgcgctt ggaattcgtc ccaagagagc ggacacttca gatcccgcct
     6181 gagtaaatcg gaacatgttg tcgacaaaca tcaaaacgtc ttgattttct tcgtcgcgaa
     6241 agtattcagc aatagaaagc gcagttaatg caactcgtgc acgcgctcct ggtggttcgt
     6301 tcatttgacc gtacaccaat gaggttttgt taattactcc ggattctttc atttccatcc
     6361 agagatcgtt tccttcgcgc gtacgctctc cgacgcccgc gaataccgag taaccaccat
     6421 ggtgggttgc aatattatta atcaattcca tgataagaac cgtttttcct acgcctgcac
     6481 caccgaacaa gccaattttt ccaccgcggg catatggcgc gagaaggtcc accactttaa
     6541 tcccggtaaa aaatggttgt actgttacgg attggtcccg aaaagctggc gcagaacggt
     6601 gaatcggata atattttttt gctgtaactg ggccagcctc gtcaaccggc ttaccaatta
     6661 cgttcaaaat tctacccaga gtctcacggc ccactggaac cgaaatttga gcgccggtgt
     6721 cagtagcgtc cataccacgc actagaccat cggtgctgtc catggctaca cagcgaacgg
     6781 tgttttcacc taaatgctgt gcgacttcta aaaccaggtt cccggctttg tcactcaacg
     6841 ccggattgct cacccgtact gcgttgaata ttgctggaag tcgaccggat tcaaaattaa
     6901 cgtcgacgat cggaccgatt acttgtttta tttttccgac actatgttgg gatgttgctg
     6961 acatactttc tggtctccct tttcctaagc tttttgggct tctgcgcccg aggtaatttc
     7021 aagcaactct ttcgtaatgg ctgcttgtct ggttttgtta tagagaagtg tgattttttt
     7081 gatcatttcg ccggcattct tagatgcgtt ttccatagcc gacattcgag cggcgtgctc
     7141 actggccaaa gcgtcataaa caattcggca aatctgagtg gcgaaatgtt tcggcaaaat
     7201 tccgtctaga atatctcgca cagttggttc gtagagagtc atttccgact cggtggttga
     7261 gctttcgcca agagtaccta ctggcaaaaa gctctcatcc ataaccgact gactaatggc
     7321 tgacttgaat tcgctgtaaa taaagcgaat atggtcgtac tgcccgctca agtataacgc
     7381 gatcaattct tctgccaatt gtgtggcttt tggcatgcga aagccggcgt aaaaatcttg
     7441 ataatactta cccggttttc ggtttttgaa gtattcatat cccttttttc cgatgaatgc
     7501 gaaatcgcac ttctcgtatt taggggcttc atttttgata aatcgtgccg ccgtttttat
     7561 gacgttggcg tttaaggcgc cgcaaagtcc gcgatcagat gtcaccacaa aaactaggca
     7621 gctttttatc tcgcttttag cctctaagag cggatgtgac tcctcgcccg aagcctttac
     7681 agcctgcgct aaagagccaa cgacttttga catttctcgc gaataaggac gcagagaaac
     7741 aatattctcc tgcgctcgac gcaacttcgc agccgaaacc attttcatgg ctttcgtaat
     7801 ctgttgcgta ttttttacgc tcgaaattcg ttttcgtagg tctttggcgc tagccatttt
     7861 ttactcaccc gttccaaaaa cagtctcaaa ttccttaagc gctttgatga gctttgtttt
     7921 ggtagcatca tcaagtttgc cggtggtcac gatatcctta caaacatctg gatgtttcgc
     7981 gtccaggaat cggtaaagtt cctcttcgta acgtcccaat ttattgagcg gcagatggtc
     8041 gagatagccg ttagttcctg cgaaaatcat aataatttgt cgctcaactg ccattggtgc
     8101 gtatacgcct tgcttgagca cttcaacaag ccgcgcgcct ctgttcagct gcttttgggt
     8161 agctgcatcg aggtcactgc cgaattgagc aaatgcagct ttttctcgat attgagcaag
     8221 gtcaagacgt aatgtaccgg caacttgttt catggcttta atttgggctg ctccaccaac
     8281 gcgagatact gaaagtccca cattcactgc ggggcgtatt ccgctataga acaaatctga
     8341 ctccaaataa atttgtccgt cagta
//
LOCUS       DAOMXO010000016         8050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_4685_length_8050_cov_4.622014, whole genome shotgun sequence.
ACCESSION   DAOMXO010000016 DAOMXO010000000
VERSION     DAOMXO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4685_length_8050_cov_4.622014"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLH57_00715"
     CDS             complement(<1..213)
                     /locus_tag="PLH57_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01159.1"
                     /translation="MKILTTLLLGLSLIGSANADESVTVYALPVTKAQVGMLGMRHPY
                     NYNSGMDRGTDLQLLIIQSGKLSKKAK"
     gene            355..1911
                     /locus_tag="PLH57_00720"
     CDS             355..1911
                     /locus_tag="PLH57_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRK01160.1"
                     /translation="MRVLAFALSLMFGPELLTPTTTAAAEISPTPKPQNQAMITLLHI
                     NDAYELAPIQGRGGLARIKGLSEQIRRHHEKPENVLLLHAGDFLAPSLDSQIFRGTQM
                     IDVMNRMGFDAVTLGNHEFEYGPETLLERMATAKFPIVTSNIHQINSFGFGNAHRFLL
                     IERNGIKIGIFAVTTPSTTQSSFPGPNVVFDDVIPAAKKAVRQLQDRGAQFVIALTHL
                     GIAEDRALARAVDGVHLILGGHDHSPMHIEENGVQIIKAGTDAQYLGRVDLVFTDGGA
                     VSSKQSSLIPITAETPSDPDLDALITNYVNEVQQKTHHPLAATEVDFEVDQDVVRTKE
                     TAVGNLLADAFRERFKADLAIVGGGTIRGNRLFKAGESISLSDIRSMIPWAGTLAKIK
                     ISGATLLRTLEHSINTIPSSSFPQVSKNVRLEFDPSRSPGNRIVTLTLDGIPVSADQQ
                     YTLAIDNYRINGGDGFGYLAQESEILIGDDAKVGEFDALVDAIRGKTIAPVVDGRVKC
                     VDILKSGSES"
     gene            complement(1934..3076)
                     /locus_tag="PLH57_00725"
     CDS             complement(1934..3076)
                     /locus_tag="PLH57_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI"
                     /protein_id="HRK01161.1"
                     /translation="MNKDQTIPKMSRELCSRLLSDLEKAKRPVLLATKCALDNARYDH
                     KVGYLQPGNKKVRTELNVSSVKKMSRAIFMLEILLRNLDTGSVNTKRELYYISKGEIK
                     HNPKLKPLDFGDQDESDATIDFICEMLEIYREEVNCYANDRGGQTYSQHLVVTETLPD
                     GEKAVIDLSKLGTSPFQPKNRPQNLKLTTKKKVDFALIVESEGTANTLVANGFTKRHN
                     AIVIGAQGVPSNAVRGWAKLIQDQLRTPMFFFGDLDAYTMQNIYRTLKAGSAASLIRN
                     SDYSAPDVHFLGVLPEDIKKYDLNDYPVKENDAQEVRALKKADDALKNDPFFQDKRNK
                     GCSEILRWLLKNKRRCEQQAYFTVDPKDPTMPEKIIVDKIKKGQYV"
     gene            complement(3085..5007)
                     /locus_tag="PLH57_00730"
     CDS             complement(3085..5007)
                     /locus_tag="PLH57_00730"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HRK01162.1"
                     /translation="MRGAIIAGSINRLVWSGKERVHFLAQSWGYPYSRGMAEEKKSKK
                     GQISSASTAEYFSKNLQQVGFSSLPKAVLTTLKEAIDNSLDACEDAGILPQIYIEVEK
                     VAAGTLKNADRIRVKVEDNGPGIDPDDLSKVFGEYLASSKFGRGRCSRGQQGIGISAA
                     TTWAQLTNAAGATVITKTAQMRKAVKALVEVDIKHNRGVLKNKETIDWDRPHGVSVEF
                     LIDGRIQLNGEGGLLTYLTGTALVNPHITLSYKLPELGVQKLERLTKEIPEIPQATEP
                     HPHTMKLGEFIAHSHLFGRVKTSAWLKKGFSRVSDASIAELKKAGLKPALFEKNVDAL
                     SEAEFRELFSAIQNTPLMAPSTRSVLAIGEASLSKAIQRLGEVDFFSVVSRKPTIADF
                     KPVQVEVAIARLLNRAVGDDAETPVQVLRFANRVPLQFDKAACAIVKAIESVNWRAYQ
                     LAQPRGGLPSGPYVMAVSVVSPFIKFKNASKETIDASEELVEEIRRALIQAGQRLSRH
                     IRREVKANDLEEKLRHIEQFCPILVDGLCRITNAPEARKKRAEDGLVKILGRDAREAQ
                     TELKEADAIVTAAIAKSNKNKKPDDDEGGDSDGSENGKQKKSKSGAREKAKPAAVRAS
                     SDDDDSDGGGQTSFNL"
     gene            5117..6295
                     /locus_tag="PLH57_00735"
     CDS             5117..6295
                     /locus_tag="PLH57_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01163.1"
                     /translation="MLSNDAVNISWNHPVLFSEPDFSAYEKKSTLLLRTGFPELAHAT
                     ENLNHNIHDPEAHAALLVALRPRLLSRGLNIMEPVWDASDPIPTLLARVRASRTRIDI
                     DRSEGADHFHGFVDDAEGWNTRIMLDTSAFIDRNSTRAWVQRRHLHLLELAPPFIQWT
                     QRTLSPSTIHELVHLAFAFQRSADLAGEIPLNALPPIFLVTVPKKNILKASRELGVYA
                     TERSPSHSTDEADAHLATSLVHLAIAEKSVEQLSQLSRLDLLGAHMHAKEAISALETA
                     RGILDHDHRVFKKALKELETFRGGTLVKTEDKVLDRRYITKSGIVLSFSRAQASSRFN
                     FPTFAGDTRHDSELARAMQKLLALQIAHYDLLLAKMKRLLRPAGVYLFDINQRMSELP
                     "
     gene            6404..7168
                     /locus_tag="PLH57_00740"
     CDS             6404..7168
                     /locus_tag="PLH57_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK01164.1"
                     /translation="MRVLLTGFLFFSLLSHFSAAFARTHDAIKQSGVLRVAVDGMTPP
                     FNYYKGKELSGFEIDLVNEMAKRLGVKVEWSVQTFNTLLVGLGQDRFDLIASSHAITP
                     ERQKVADFAEPHYCTGAVLVTKSGGPKTKSDLVGKVGVVPVGTVYYNALKNVPGIKEV
                     RTVPSETDGLQTLLNGRADVWVTDQFVAMDVVKKHQKESLTVGESIMDQQNAMVVAKG
                     NDSLRNAINVIERNMMKDGFYKKLSMSYFGRDIRCK"
     gene            7165..7899
                     /locus_tag="PLH57_00745"
     CDS             7165..7899
                     /locus_tag="PLH57_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019008213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRK01165.1"
                     /translation="MKQLIHLGFAFAAIAVIFLILNFALPLCPEPIGSRADLFITGAK
                     LTLVLTFYAGVIGLLLGTLAGVAKLQKNLLLRGVATFYTWLFRGTPLLVQILFIYFAL
                     PVVSPSFQFDEQTAAIIALALNVGAYNAEAIRGGLIAVPKGQTEAAVSLGLSPVQTLV
                     WITLPQAFRISIPPLVSNLVALLKDSSLASSIGLLELSLVGNRVSSETFSPVPVLLTV
                     AGIYLVITSVLTAAALPLEKKVRGEK"
     gene            7896..>8050
                     /locus_tag="PLH57_00750"
     CDS             7896..>8050
                     /locus_tag="PLH57_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK01166.1"
                     /translation="MSVLVTRDVHKTFGTQPVLNGVSFTADKSEVIAIIGPSGSGKST
                     FLRTLNGL"
BASE COUNT         1906 a         2140 c         1871 g         2133 t
ORIGIN      
        1 cttagctttt ttggacagct ttccggattg aatgattaac aactgcagat ccgttccacg
       61 atccattcct gagttgtagt tataggggtg acgcataccg agcatcccca cctgagcttt
      121 ggtcaccggt aaagcgtaca ctgtaacgct ctcatcggcg tttgcgctcc cgatcaaact
      181 taaacccaac aataacgtgg tgagaatttt catgaaaatg gttccctttc gaaaaaaata
      241 cagttccgag taacgcggcg cttaatatca aaatctcctg caacttgaaa ggcaaacgcc
      301 attctgataa aaaaagtcga ttttttaagg atcctcataa ccgacggagt ccgaatgcgc
      361 gtgttggctt ttgcacttag cttaatgttc ggtcccgagc ttttaacccc cacgacaact
      421 gcagcagccg aaatttcacc gactcccaaa cctcagaatc aagcaatgat caccctttta
      481 catataaacg acgcatatga actggctcca atccagggcc ggggtggact tgcccgaata
      541 aaagggctta gcgaacaaat tcgacgccac catgaaaaac cggagaacgt gctgttgctc
      601 catgccggcg attttttggc cccttcgctg gattctcaga tttttcgggg cacccaaatg
      661 attgacgtca tgaatcgtat gggtttcgac gcggtcacct tgggtaacca tgaattcgag
      721 tacggcccgg aaacactctt agaaagaatg gcgaccgcca agttcccaat tgttacttcg
      781 aatattcacc aaattaatag ttttggtttt ggcaacgctc atcgattctt attgattgaa
      841 cgaaatggca tcaaaattgg aattttcgca gtcaccacac ctagcaccac gcagtcttct
      901 tttcccggac cgaacgtggt gtttgacgac gttattccgg cagcaaaaaa agcggttcga
      961 cagttacagg accgaggcgc gcaatttgta atcgccctca ctcacctagg aatcgcagag
     1021 gaccgcgcac tagcccgcgc cgttgacggc gttcatctaa ttcttggtgg ccatgatcat
     1081 agccccatgc acatcgaaga aaacggcgtg caaataatta aagccggtac cgacgctcag
     1141 tacttgggac gagtagattt ggtttttacg gatggtggag ccgtgagtag caaacaatcc
     1201 tctttaatac ccataactgc tgagactccg tctgatcctg atcttgacgc cctcattacg
     1261 aattacgtga acgaggttca gcaaaaaact caccatccct tggcagccac agaagtggac
     1321 tttgaggtgg accaagatgt cgttcgcaca aaagaaaccg ctgtgggaaa tcttcttgct
     1381 gacgcttttc gtgaacgctt caaggccgac cttgcaatcg tcggaggtgg cacgattcgc
     1441 ggcaaccggc tttttaaggc gggagaatct atttcgcttt cggacattcg cagcatgatt
     1501 ccctgggccg gcacgttggc caagattaaa atatcgggcg cgactttgct gagaacactg
     1561 gaacactcga tcaacaccat tccgagttcg agttttcccc aggtttccaa gaacgtccga
     1621 cttgaatttg atccgtcccg ttcgcccggt aacaggatcg taactctcac gctcgacgga
     1681 attcccgtga gtgccgacca gcagtacact ttagcgatcg acaattaccg aattaatggc
     1741 ggtgacgggt ttggatacct cgcacaggaa agcgagattc taatcggcga cgacgcaaag
     1801 gtcggcgaat tcgatgcttt agtcgacgcc attcgcggaa agacaatcgc accagtggtc
     1861 gatggccgcg ttaaatgcgt cgatattcta aaatccggtt cagagagctg acccggcgta
     1921 agcgacgcct aaatcagacg tactgacctt tctttatctt atctacgatg attttttctg
     1981 gcatcgtcgg gtccttcgga tcaaccgtaa agtacgcctg ttgttcgcaa cgacgtttgt
     2041 ttttaagcaa ccaacgaaga atttcagaac accctttatt gcgtttatct tgaaaaaagg
     2101 ggtcattttt aagtgcgtcg tcggctttct ttagggcgcg cacctcttga gcgtcattct
     2161 cttttacggg atagtcgttt agatcatatt ttttaatgtc ttctggcaac acccctaaga
     2221 aatgaacatc gggggccgag tagtcggagt ttcgaataag actcgctgcg gaccccgcct
     2281 taagtgtgcg ataaatgttt tgcatggtgt acgcgtcaag gtcgccaaag aaaaacatcg
     2341 gcgtccgcaa ttggtcctga atgagtttag cccagccgcg cacggcgttt gacggcaccc
     2401 cttgcgcacc aatcacgata gcattgtgcc gtttggtgaa accattggcc acgagcgtat
     2461 tggcggtacc ttccgattcg acaataagag caaaatccac tttctttttt gtcgtcagtt
     2521 ttaagttttg cggtcgattt tttggctgaa agggacttgt tccgagctta gaaaggtcga
     2581 tcactgcctt ttcgccgtcc ggtaaagttt ccgtaacaac caaatgctgg gaataggttt
     2641 gcccgcctcg gtcgtttgcg tagcaattca cttcttcgcg ataaatctct aacatttcgc
     2701 agatgaagtc gatcgtagcg tcactttcgt cttgatcccc gaaatccagc ggtttgagtt
     2761 ttggattgtg cttaatctcg cctttcgaaa tatagtacaa ctcgcgtttg gtatttacgc
     2821 tcccggtatc gagatttcgt aaaagaatct ccagcatgaa aattgcgcgc gacatttttt
     2881 ttacagaact tacgttcagt tctgtacgaa cctttttgtt accgggctgc aaataaccga
     2941 ccttgtgatc gtatctcgcg ttgtccaatg cgcatttggt ggccagcagc actggacgtt
     3001 tggccttctc gagatcactc aacaagcggc tacaaagttc tcgcgacatt tttggtatcg
     3061 tttggtcttt attcatgtgt tcctctacaa attgaaacta gtttgaccgc cgccatcact
     3121 gtcgtcgtcg tcactgctag ctcgtacggc agccggctta gctttttcac gggcaccgct
     3181 tttagatttt ttctgttttc cgttttcgga gccgtcacta tccccgcctt cgtcgtcgtc
     3241 cggcttttta tttttgttac ttttggcgat cgctgcggtc acaatcgcat ctgcttcttt
     3301 taactcggtt tgtgcttccc gagcatcacg accaagaatt tttaccaacc catcctcagc
     3361 gcgttttttg cgagcttcag gagcattggt gatccgacaa agaccatcga ctaaaattgg
     3421 gcaaaactgt tcgatatgcc gcagtttttc ttcgagatca ttggctttga cttcgcgccg
     3481 aatatgccgt gaaagtcgtt gccccgcttg tataagcgct cttcgaattt cttctactag
     3541 ctcttcactc gcgtcgatgg tttctttgga tgcgttttta aatttaataa acggcgacac
     3601 caccgacacg gccatgacgt aggggcccga aggcaaccca ccgcgcggct gcgccagttg
     3661 atatgcgcgc caatttacac tttcaattgc tttaactatt gcacatgccg ctttatcgaa
     3721 ctgtagcggc acccgattag cgaaacgcaa aacctgaacc ggagtttcgg cgtcatcgcc
     3781 taccgcgcgg tttaacaatc gcgcaatcgc cacttcaacc tgaacgggtt taaagtccgc
     3841 gatggtgggc ttgcgagaaa ccaccgaaaa aaaatctact tcgcccaatc tttgaattgc
     3901 tttcgagagc gacgcctcgc cgattgcaag caccgatcga gtgctaggcg ccatgagcgg
     3961 agtattttga attgcactga aaagttctct aaactctgct tcactgagtg catcaacgtt
     4021 cttttcaaac aatgcgggct ttagcccagc tttctttaat tcggcaatcg atgcatcgct
     4081 gactcgcgaa aaaccttttt ttagccaagc cgaagttttc acccggccaa acaagtgaga
     4141 gtgcgcaatg aattcgccca acttcatggt gtgggggtgc ggttcggtgg cttgaggaat
     4201 ttctgggatc tctttggtca gacgttcaag tttttgcacg cccagttcag gaagtttata
     4261 gctaagcgtt atgtgggggt tcaccagagc tgtgcccgtg aggtatgtga gcaacccgcc
     4321 ctccccgttg agttgaatgc gcccgtcgat caagaactct accgacacac cgtgaggacg
     4381 gtcccaatct attgtttctt tgttttttag tacaccgcgg ttgtgtttaa tatccacttc
     4441 aaccaacgcc ttgacggctt ttcgcatctg agcggttttg gtgatcaccg tggcaccagc
     4501 ggcgttagtc aattgcgccc aggtcgttgc agctgaaata ccaattcctt gctgcccccg
     4561 agagcaacgc ccgcgcccaa acttgcttga ggccaagtac tcaccaaaaa ctttcgataa
     4621 gtcgtcagga tcgattcctg gtccgttgtc ttcgacctta acccgaatgc ggtcagcgtt
     4681 ctttaaagta ccggctgcaa ccttttcgac ctcaatgtaa atctgcggca gaatcccagc
     4741 atcttcacaa gcgtcgagcg agttatcgat ggcctcttta agtgtcgtta gcaccgcttt
     4801 cggaagggag ctaaagccca cttgctgaag gtttttggaa aaatactccg ccgtactagc
     4861 cgagctgatt tgcccttttt ttgatttttt ttcttctgcc atgccacgag aataggggta
     4921 accccacgat tgtgcaagga aatggactcg ctctttacct gaccacacca atctgttaat
     4981 agagcccgca atgattgcgc cccgcattcg caccgctttt tggtctattc agctattcct
     5041 cctcttgggg gcgccgagca cttttgccaa tagtgcgctg gagtcatgcc gcgacctact
     5101 tggaagctac cgccacatgc tctctaacga cgcggtaaac atcagttgga atcaccccgt
     5161 actctttagt gaacctgatt ttagcgccta cgaaaaaaaa tctaccctgt tgctccgaac
     5221 agggtttccc gaacttgcgc acgcgaccga aaatctcaac cacaatattc acgacccgga
     5281 ggctcacgct gctctacttg tggcgcttcg cccccggctt cttagtcgcg gcctcaacat
     5341 catggaaccg gtttgggacg caagcgaccc tattcccacg ctccttgctc gagtgcgagc
     5401 ctcccgcact cgaatagata ttgatcgcag cgaaggagca gatcattttc acggcttcgt
     5461 cgacgacgcc gaaggttgga acactagaat catgctcgat acctcggcgt ttatcgaccg
     5521 caactcgact cgcgcctggg tgcagcgccg ccacctccat ttgcttgagc tcgctcctcc
     5581 attcattcaa tggacccagc gcacgctcag cccttcgacg attcacgaac tcgttcacct
     5641 agcgtttgca ttccaaagaa gcgcagacct tgcgggcgaa atccccctca atgcactacc
     5701 gccaatattc ttagtcacag ttccaaagaa aaacattctg aaagcctcac gcgaactggg
     5761 cgtatatgcc accgaacgat ctccgtctca ttcgacggac gaggcagacg ctcacctcgc
     5821 gacttcgctg gtgcatcttg caattgccga aaagtcggtg gagcagctct cgcaactaag
     5881 tcgcttggac ctactcggtg cccacatgca tgcaaaagaa gccatctcgg ccttggaaac
     5941 tgcccgaggg attttggatc acgatcatcg agttttcaaa aaagcgctca aagaacttga
     6001 aaccttccgc gggggcactc tggtgaaaac tgaagataaa gtactcgacc gccgctacat
     6061 tacaaagtcc gggatcgtcc tttctttttc tcgggcccaa gcatcttcga gatttaattt
     6121 ccccacattc gcgggcgaca cccgccacga tagtgagctt gctcgcgcga tgcagaaact
     6181 tcttgcgtta cagatcgccc actacgatct ccttctggcg aagatgaaac gtttactacg
     6241 ccccgccggt gtctacctat tcgacattaa tcaacgaatg agcgagcttc cctgaaaccg
     6301 gggaccaaat taattgcaga aaatcttgaa attattcttg gacaacggcg cctggcggcc
     6361 taaattggtt tcgagtttcg acagggatca ataggagatt ctaatgcggg tattgttgac
     6421 tgggttttta ttttttagtt tgttgagtca tttttccgcg gcatttgctc gtacccacga
     6481 tgcaatcaaa caaagcggtg tgcttcgagt ggcggttgat ggcatgacgc ctccatttaa
     6541 ttattataaa ggcaaagagc tctctggctt tgaaattgac ctcgtcaatg aaatggccaa
     6601 acgcctaggc gtcaaggtcg aatggagtgt gcaaaccttc aacaccctac tggtcggact
     6661 tgggcaagac cgattcgatc tcatcgcctc gtcccacgcg atcacgcccg agcgccagaa
     6721 ggtcgccgac ttcgctgagc cccactattg tacgggtgct gtactagtaa ctaaatctgg
     6781 cggccctaaa accaaatctg acctcgtagg aaaagttggg gtcgtgccgg taggaaccgt
     6841 ttattacaac gccctcaaaa atgttcctgg cataaaggaa gtaagaactg ttcctagtga
     6901 gacggacggc cttcaaaccc tactcaatgg ccgggccgac gtttgggtaa ccgaccagtt
     6961 cgtcgcgatg gacgtggtta agaaacacca aaaagaatcg ctcacggtcg gagaaagcat
     7021 catggatcaa caaaacgcga tggtcgttgc caagggcaac gattctctac gaaacgcgat
     7081 caacgtgatc gagcgtaaca tgatgaaaga cgggttttac aaaaaactca gcatgtccta
     7141 tttcggtcgc gacatacgct gcaaatgaaa caactcatcc acctcggttt cgcgtttgcg
     7201 gccattgccg tcatttttct aattctgaat ttcgctctgc ccctttgccc tgaacctatt
     7261 gggtcccgcg ccgacttgtt tattactggc gcaaaactca cgcttgtcct cacattctac
     7321 gcgggcgtta tcggattatt gttaggaacg ctcgcaggcg tagccaagct tcaaaaaaat
     7381 cttctactgc gtggagtggc cactttttat acctggctat ttcgtggcac tccgctccta
     7441 gtacagattc tttttattta cttcgcattg ccagttgttt cgccatcgtt tcagtttgac
     7501 gaacaaactg cagcgatcat tgcattggcg cttaacgtgg gcgcttacaa tgccgaagcc
     7561 attcgcggtg gccttattgc cgtgcccaaa ggtcagaccg aagcggccgt tagtcttggc
     7621 ctctcgcccg ttcagacgct cgtgtggatt actctacctc aggcctttcg gatttcgatt
     7681 ccccctttag tgagcaatct agtggcactt ttaaaagaca gctcccttgc ctcgagtatc
     7741 gggttgctcg aactatcgtt ggtaggaaat cgcgtttcga gtgaaacgtt ttcaccagta
     7801 cctgtcttgc tcaccgtggc tggaatttat ctcgttatca ctagtgtgct taccgctgct
     7861 gcgttgccgc ttgagaaaaa agttcgaggt gaaaaatgag cgtactcgtc actcgcgatg
     7921 tgcacaaaac atttggcact caaccagtgc tcaatggcgt ttcatttact gccgacaaga
     7981 gcgaagtgat cgccatcatc ggaccctcgg gatctggtaa atccaccttt ctgcgcacgc
     8041 tcaacgggct
//
LOCUS       DAOMXO010000017         8028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_4710_length_8028_cov_5.335758, whole genome shotgun sequence.
ACCESSION   DAOMXO010000017 DAOMXO010000000
VERSION     DAOMXO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4710_length_8028_cov_5.335758"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLH57_00755"
     CDS             <1..398
                     /locus_tag="PLH57_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01167.1"
                     /translation="RPVHQHARRRLDSCAEHRAGIGRLIYKAREDLVRKLGLLRIRAG
                     ARLRGYHAYASKMTPDEYVLEVVKGKLNDPTLSFQLKRKFHVLRVVSNYLRHDPESMG
                     FAAVIEWINPDVATEADYAHLRESKFYRD"
     gene            419..1045
                     /locus_tag="PLH57_00760"
     CDS             419..1045
                     /locus_tag="PLH57_00760"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HRK01168.1"
                     /translation="MNHRPFCLGCRRAQKVCQCADVRRFEADPVFVILMHPVERRMKT
                     GTGRLAHLCVSNSILIEGANFANHPRVSELISDPRYFPVLLHAGAEATSLQNLDLPKD
                     RRLLIFIIDTKWSMVKTVLNQSPNLKALPKVCLVPSRPSGFHTRRQPKPNYLSSIEAI
                     HHVVEQLSPGRPEHAVLLEVFDKMVERQLGFQSHPTHRHRFAKPRKTL"
     gene            1110..1409
                     /locus_tag="PLH57_00765"
     CDS             1110..1409
                     /locus_tag="PLH57_00765"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRK01169.1"
                     /translation="MYEATEAFSSLSQETRLKIFRLLIEYGSDGLVPGKIASRLKIPN
                     NTLSFHLSHMSNAKLVTSKRNGRSIVYYANTDLIEELIEYMVANCCVRSKERTCK"
     gene            1406..1849
                     /locus_tag="PLH57_00770"
     CDS             1406..1849
                     /locus_tag="PLH57_00770"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HRK01170.1"
                     /translation="MKRFHIHVGVRDLEESVRFYSTLFGQQPSKRKDDYAKWMLDDPR
                     LNFAISTRIGGTGVDHLGIQVENPGELSEIAERLKNADLSVHDEGETTCCYSKSNKSW
                     VEDPSGVAWEAYQNLADAEIYREAPKTGEANEKTGCCVAEESSCC"
     gene            2031..5156
                     /locus_tag="PLH57_00775"
     CDS             2031..5156
                     /locus_tag="PLH57_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRK01171.1"
                     /translation="MLDALIRFSLRHRMLVVVTAAMVLVYGALVLRELPVDVFPDLNR
                     PTVTLMTEARGMAPEEVETLVTLPLETALGGLPGVDRVRSSSGVGLSIVYVEFDWGTD
                     VYRNRQLVAEKLLLVQERLPKNTTPSMTPIASIMGEIQLLGLVSPGGELSPIELRTLA
                     EWVIRPRLLAVPGVAQVVPLGGGLKQFQILISAPKLQRHRLSLEEVQHNLEHLSQNTT
                     GGFVDLEGREHLIRTIGTTRSLEEIQNSVVGLHFGKPVLVRDIATVQVGAQVKRGDAS
                     VNAAPGVVLSVQKQPGTNTVELTQRIDAALVELEKGFPKGVRLERDLFKQAHFIEAAI
                     RNVKEALRDGTILVVLVLLIFLANFRTTAITLTAIPLSFVLTAIVMKMFGITINTMTL
                     GGLAVAIGELVDDAIVDVENVYRRIRENRAQSNPSSLLKVVFDASSEVRNSIVLATVI
                     VVLVFVPLFSLGGIEGRLFIPLGVAYVVALAASLLVSLTVTPVLCSYFLTKDASIEKK
                     ETFFVTALKRLDRRILNWAFDHPKVVITFVAALFLAAAATVPFMGRDFLPKFNEGTAI
                     VNLLAQPGISLEQSNALGSRAEQLILEIPEVKSVSRRTGRAEMDEHAEGVHSSEIDVD
                     FKQTGRNREVVFQQIREKLAPLGASVAIGQPISHRLDHLLSGVRAQLAIKLFGADLGV
                     LRQKAAEIENALKEIHGLVDLHVEQQVLIPQVKIHLLRDEAAELGIVPGELATMLELA
                     LNGEVVAQIIDEQRIFNVFMRFDDESRSNLELMKKTIIKIMPDGSAIPLEQVADVFES
                     TGPNLINRENAQRRIVIQANSSTGDLDSIVEQVRTAIQAKVKLPDGMYIQYGGQFESQ
                     KRASQKILLLSLLSLAGIFLVLYAHFKSVFVSVQIMLNIPLALIGSLFFLFLTERIFS
                     VATLVGLVALCGIASRNGIMMLSHYLHLMKHEGETFTREMVIRGSLERLVPVLMTALT
                     AALALIPLAVSHGAPGREILHPVAIVIVGGLLSSTLLDLVVTPTVFFNYGRRSAEASL
                     ERTKKEKGEEL"
     gene            5153..5590
                     /locus_tag="PLH57_00780"
     CDS             5153..5590
                     /locus_tag="PLH57_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01172.1"
                     /translation="MKHWKILLVVTALTSGSLGFSHEGHDHSPGQVQAPNNGQLQKTK
                     DIYVEVAGTKEEVKIYFYDTKLQPLGHSGIKVRAELRFPERTKKNQSSHKSKAKKNVA
                     IELKDQGDYFSGPVVVGSAHRYLVDLDLVLNGRSEKLTFQVEP"
     gene            5590..6837
                     /locus_tag="PLH57_00785"
     CDS             5590..6837
                     /locus_tag="PLH57_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRK01173.1"
                     /translation="MLGNVLILVISSIAAAAGDRLVVRFEDLPRLLQFANGHVKGAGQ
                     KAEAADARTSFLLKSYLPTLKAEAGRERFETGPFKPQTQSYGSVEASVNLFRGGRDAL
                     ESEARNAQRELADGEAKQTYLQELSDARKLYWQLVYQREVLSLLRSALSQNEKSRATA
                     MKRIAGGLATQSDRLEFEISQIQFEQDIAKSELSIANEQRKLASLLGRPIDTVIETAT
                     FVPHDHDDSIGAESLQPERHPNVRVGRAIQTYFDAQSKQAGRWWLPSLEVYGTYALYT
                     LRERDHFARKDRFDTAVGARLNMELFDGLRSASESKAHAFEARAGELSAAQSARELAA
                     QFENAKQELLVTHQLIHDGERNVEKGQEYLRLTLAEYGRGSKNSPDVLSANQKYVDFK
                     RRYAELRRDYQLVRAELMALLGK"
     gene            complement(6847..7734)
                     /locus_tag="PLH57_00790"
     CDS             complement(6847..7734)
                     /locus_tag="PLH57_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRK01174.1"
                     /translation="MFRVVVAGAGPWGRILIKNILGAPDYELAGVVSRNSETPKLVPT
                     STQVAGDLKTITVPYDGLVVATPPEVHADLLRQGLAKRVPVFVEKPLALSLNETEQIH
                     AVISAANAAVMVDHIHLFNPAYEQLRSKALDEGVCSIESVGGGPGPFRSYSALWDYGP
                     HDLAVCFDLMGSPPLAVSAVQAHRANGGLSVQAVLHFSHDRTARVRFGNNFERKIRTT
                     KVLTRKGNTYLFDDIANKTQNAMVATPLQNSVVPTPLQNALAIFRDDHILREDRRWGT
                     GHALEIARALDGIERQLPN"
     gene            complement(7736..>8028)
                     /locus_tag="PLH57_00795"
     CDS             complement(7736..>8028)
                     /locus_tag="PLH57_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar epimerase"
                     /protein_id="HRK01175.1"
                     /translation="FLRVLALGESGEPYNIGNPTPEISMLQLCEKLTTILKRPVKFDL
                     RPYPDSYPGDEPMRRCPDISKARRGVGYEPTVSLDDGLTRFFRWAFDTYTKI"
BASE COUNT         2004 a         1906 c         2128 g         1990 t
ORIGIN      
        1 ttcgacccgt gcaccaacac gcacgtcggc gcctggattc ttgcgcagaa catcgggcgg
       61 gcattggccg gttgatctat aaggcacgcg aagacttggt acgaaaactg gggctactta
      121 gaattcgggc aggagcaaga ttacgaggat atcacgctta cgcatcgaag atgactcctg
      181 acgagtacgt gcttgaagtg gttaagggga aattgaacga tccaacacta agttttcagt
      241 tgaaacgaaa gtttcatgtg ttgagggtcg tctcgaatta tttgcgccac gatcctgaaa
      301 gcatggggtt cgctgcagtg attgaatgga tcaacccaga tgtcgcgacc gaagcggatt
      361 atgcgcattt gcgagagtcc aaattttacc gggattaaat ttgaatcgag ttttagctgt
      421 gaaccaccgc ccattttgtc ttggttgtcg tcgtgctcag aaagtgtgtc agtgtgctga
      481 cgtgcggcgc ttcgaagctg atccggtttt cgtgatcctc atgcacccgg ttgaacggcg
      541 aatgaaaacc ggtaccggcc gcttggctca tctctgtgtg agtaactcaa tcttaatcga
      601 aggcgcgaat ttcgcgaacc acccgcgagt gagtgaactc atcagcgatc cgcgatattt
      661 tccagtgttg ctccacgccg gggccgaggc gaccagcttg caaaacctcg acttgcccaa
      721 agatcgtcgg ctacttattt tcattattga taccaaatgg agcatggtta aaaccgtgct
      781 caatcagtcg cccaacttaa aagcactgcc caaagtttgt cttgtaccga gtcgtccttc
      841 ggggtttcac acgcgccggc agcctaagcc gaattacttg tctagcatcg aggcgattca
      901 tcacgttgtg gaacagttga gcccgggaag gccagagcac gctgtgttgt tggaggtgtt
      961 cgataaaatg gtggagcgcc aattgggctt tcaaagtcat cctactcatc ggcaccgatt
     1021 cgcaaaaccc agaaaaacgc tttaatttca aagggtattc accactggtt gacatgattt
     1081 gatatttcga ttattctaga aatatggaaa tgtatgaagc aaccgaggca ttctcttcgt
     1141 tgtcacaaga aacaaggctc aagatcttca ggctgctcat tgaatacggg agcgatggat
     1201 tggttcctgg aaaaatagct agtcggctga agattccgaa taacaccttg tcgtttcatc
     1261 tttcgcacat gagcaacgcg aagctggtga cgtcgaaacg aaacgggcgt tcgatcgtat
     1321 attacgccaa caccgacctc attgaggaac tcatcgaata catggtcgca aactgctgcg
     1381 ttcgcagcaa agaaaggaca tgcaaatgaa acggtttcac attcacgttg gagttcggga
     1441 tctcgaagag tcagttcggt tctattccac gctttttggg caacagccat cgaaacgcaa
     1501 ggacgactac gctaagtgga tgttagacga tccgcggcta aattttgcga tttccacccg
     1561 gattggtgga actggggtgg accacttggg catacaagta gagaaccctg gcgaactctc
     1621 cgaaatcgca gaacgactta aaaatgcaga tctcagtgtg cacgatgagg gagaaacaac
     1681 ctgttgctac tcgaaatcga acaagtcctg ggtcgaagac ccctcgggcg tcgcctggga
     1741 agcgtatcag aacttggcgg atgccgaaat ctaccgagaa gcgcccaaaa ctggcgaggc
     1801 taacgaaaag accgggtgtt gcgtagctga agaatctagt tgttgctaag gctagatggc
     1861 agaatctact gcaactcgca gttctggata aattcgatcg cgcggtcgtc ggtagtttgg
     1921 tgggtgacca ttatcagtta aaaaaaacca accataacgc gcgcttggta gcgctgcact
     1981 tttctttgtg agatattctg tttaactctg acggtttcca ggagggattc gtgctcgacg
     2041 cgctcattcg tttttcgctc cgccaccgca tgctggtcgt tgtgaccgcc gcaatggtgc
     2101 tagtttatgg cgccttagtg ttgcgagagc taccagtaga tgtttttcca gatctaaacc
     2161 gtcccacggt cacgctgatg accgaggcgc gagggatggc ccccgaagaa gtcgaaacgc
     2221 tagttactct gccgctcgag actgctttgg gaggtttacc tggagtcgat cgagttcgtt
     2281 cgtcctcggg cgtcgggtta tcgatcgtat acgtggaatt tgactggggt accgacgttt
     2341 accgtaaccg ccagttggta gctgaaaaac tgctgcttgt tcaggaacgg ttgccaaaaa
     2401 atactacgcc cagcatgacc cccattgcgt cgattatggg agaaattcag ttgctggggc
     2461 tcgtatcgcc tggcggtgaa ttgagcccaa tcgaactgcg caccttggcc gagtgggtga
     2521 ttcgcccacg actattggcc gttccggggg tagcgcaagt ggtgcccctc ggtggagggc
     2581 ttaagcagtt ccaaattctg atttcggctc ccaaacttca acggcatcgg ctttcactcg
     2641 aagaggtgca gcacaaccta gaacatctaa gtcaaaatac cacgggtggg tttgttgatc
     2701 tcgaaggccg tgaacatcta attcgcacca tcggcactac ccgttctttg gaggaaattc
     2761 aaaactctgt ggtgggtttg catttcggta aacccgtgct ggttcgagac attgcaactg
     2821 tccaggttgg tgcgcaagtc aaacgcggcg atgcaagtgt caacgccgca ccgggcgtag
     2881 tgctgagcgt gcagaaacag ccaggaacca acaccgttga gttaactcaa cgtatcgacg
     2941 cagccttggt cgaacttgaa aaagggtttc ctaagggcgt gcgcttagag cgtgatctat
     3001 ttaaacaggc ccactttatt gaagctgcaa tccgtaatgt caaagaagcg ctgcgagacg
     3061 gcactatctt ggtggtactg gttcttctga tttttctagc caattttcga accaccgcaa
     3121 tcacgcttac cgccatcccc ctttcgttcg tactgactgc gattgtgatg aaaatgtttg
     3181 gtattactat taataccatg acgctcggcg gccttgccgt ggctatcggg gaacttgtcg
     3241 acgacgcgat cgttgatgtt gaaaatgttt atcgccgcat acgcgagaat cgcgcgcagt
     3301 cgaacccaag ttcacttctg aaggtagtct tcgacgcatc gagcgaggtt cgtaactcca
     3361 tcgtactggc tacggtaatc gtcgtgttgg tgttcgttcc gctgtttagc ctgggcggca
     3421 tagaggggcg gctttttatt ccgctcgggg tagcatatgt agtggcccta gcagcgtcgt
     3481 tgctggtctc gctcacagtt acgccagtat tgtgttcgta ttttcttacc aaagacgcat
     3541 cgattgaaaa aaaagaaact ttctttgtga ccgccctaaa acggttggat cgccgaatac
     3601 ttaattgggc ctttgaccac ccaaaggttg tgattacctt tgtggcagct ctctttcttg
     3661 ctgccgcagc tacagtgcct ttcatgggcc gggactttct tccgaagttc aacgaaggca
     3721 cagcgatcgt aaatttgctg gcgcaaccgg ggatatcact agagcagtcg aacgcgcttg
     3781 gttcgcgagc agagcaatta atactcgaaa ttcccgaagt gaaatcagtg tctcgacgta
     3841 ctggacgcgc ggaaatggac gagcacgcag agggagtgca tagtagcgag atcgacgttg
     3901 attttaaaca aacgggcaga aaccgcgaag tcgtgtttca acaaattcgc gaaaaacttg
     3961 ctccacttgg ggccagcgtt gctattggcc agccaatttc tcatagactc gatcatttgc
     4021 tctccggcgt acgcgctcag cttgcaatta agctctttgg ggccgatctt ggagtgttgc
     4081 gccaaaaggc cgccgagatc gaaaacgctt tgaaggagat ccacggatta gtcgacttgc
     4141 acgtagaaca acaggtattg ataccacaag ttaaaataca tcttttacgg gacgaagcag
     4201 ccgaacttgg cattgttcct ggggaactag ccacaatgct tgaactcgca ctcaatggcg
     4261 aggtagtcgc tcagatcatc gatgagcaac gaatatttaa cgtatttatg cgatttgatg
     4321 acgagtctag atcaaatcta gagctcatga aaaagacgat tattaaaatc atgcccgacg
     4381 gttcggcgat tccactcgaa caggtagctg atgtttttga gtccaccggt ccgaatctca
     4441 ttaatcggga aaacgcacaa cgcagaatcg tgattcaagc caattcttca acaggtgatc
     4501 tcgattccat tgttgaacag gtgcgtaccg cgattcaggc caaagtgaag cttccggacg
     4561 gcatgtacat tcaatatggt ggtcagttcg agagtcaaaa aagagcgtcg caaaaaatac
     4621 ttcttcttag tttgttgtcg cttgcgggca tttttttggt tctttacgcc cacttcaaat
     4681 cagtgtttgt cagtgtgcag atcatgttga acatcccact tgctctaatc ggaagtttat
     4741 tttttctatt tttaaccgaa cgtatttttt cggtcgctac actcgtgggt ctcgtggcgt
     4801 tgtgtggcat tgccagccgt aacggcatca tgatgctttc gcattactta cacctaatga
     4861 aacacgaagg cgaaacgttt actcgtgaaa tggtgattcg tggttcgcta gaacgactgg
     4921 tgccggtgtt aatgacagcg ttgaccgcgg cacttgcgct cattccattg gcagtttcgc
     4981 acggagcgcc cggtcgagaa attctgcacc cggttgcgat agtgattgtt ggcggactac
     5041 tgagttcaac actactcgat cttgttgtta cgcctacggt attttttaac tatgggcgcc
     5101 gatcggccga agcgtcgctc gagcgcacga aaaaagaaaa aggagaagag ctatgaaaca
     5161 ctggaaaata ttgttggtgg tgacagcgct cactagtggg tcgctaggtt tttcgcacga
     5221 agggcacgat cattcaccag gacaggtaca agctccaaac aatggacagt tacaaaagac
     5281 caaggatatt tacgtagagg tcgctggtac taaagaagaa gttaaaattt atttctacga
     5341 tactaaactt caaccactcg ggcatagcgg tattaaagta cgtgcagaat tgcgatttcc
     5401 ggagcgaaca aaaaaaaatc aatcatccca caagtcaaag gcaaaaaaaa atgtggcaat
     5461 cgaactcaaa gatcagggtg attacttttc tggtcccgtg gttgtcggtt cggcgcatcg
     5521 atatttggta gacttggatc tcgtattgaa cggtaggtcc gagaagttaa ccttccaggt
     5581 agaaccgtaa tgttgggaaa cgttttaata ctggtaatct cttcgatcgc agcggcggcg
     5641 ggcgaccgtt tggtcgttcg cttcgaagat ctgcctcgat tgttgcagtt cgccaacgga
     5701 cacgttaagg gagccggtca aaaagctgaa gcagcagatg cgcgtacctc ctttttgctg
     5761 aagtcttacc ttcctacctt gaaagctgaa gctgggcggg agcgtttcga aaccggtcca
     5821 tttaagcccc agacgcagtc gtatggctcg gtcgaagcgt cggtgaacct ttttagaggg
     5881 gggcgagatg cacttgaaag tgaggctcga aatgcccaac gcgaacttgc ggacggtgag
     5941 gcgaaacaga cttaccttca ggaactctcg gacgctcgta aattatactg gcagttggtt
     6001 taccagcgtg aagtgttaag ccttttacgt tcggctttat cgcaaaatga aaaaagtcgc
     6061 gccaccgcaa tgaagcgaat tgccggtggt ctcgccacgc aaagtgatcg cttggagttt
     6121 gagatttccc agattcaatt cgagcaggat attgcgaagt ccgaactcag cattgcgaac
     6181 gagcagagaa aactggcctc cctacttggt cgtcccatcg atacggtgat cgaaaccgct
     6241 acctttgttc cgcacgatca cgacgattca atcggggcgg aaagtctgca accggaacga
     6301 caccccaatg ttagagtagg gcgtgcaatt caaacttatt tcgatgctca gagtaaacaa
     6361 gccgggcgat ggtggctgcc ttcgctcgag gtttacggca cttatgcatt gtacacccta
     6421 agggagcggg accatttcgc gcgtaaagac cgattcgaca cagcggtggg agcacgactg
     6481 aatatggaac ttttcgatgg actgcgctct gcttcagagt caaaagccca cgctttcgaa
     6541 gctcgagcag gtgagctcag tgcagcgcag tcggcccgcg aattggccgc tcagttcgag
     6601 aacgccaagc aggagctact agtcacgcat cagttgattc atgatggcga aagaaatgta
     6661 gaaaaagggc aggaatactt gcgattgacg ttggccgaat acggacgggg gagcaagaat
     6721 tcgccagacg ttttgagcgc aaatcaaaag tacgtggatt ttaaacgtcg ctacgcggag
     6781 ttgcggcggg attaccagtt ggtgcgcgcc gagttaatgg cgttgctcgg aaagtaaact
     6841 aggtcgttag ttcggcagtt gccgctctat accatctagc gcacgtgcaa tttctagtgc
     6901 atgtcccgtg ccccagcgcc gatcttcgcg aagaatatga tcatcgcgaa agatcgctaa
     6961 agcattttgc aacggagttg ggactaccga gttctgcaac ggagttgcga ccatcgcgtt
     7021 ctgagttttg ttggcaatgt cgtcgaagag gtaggtattg cctttgcggg tgagtacctt
     7081 ggttgtgcga atcttacgtt cgaagttatt tccaaaacgc actcgcgcgg tgcggtcgtg
     7141 agaaaaatgc aacactgctt ggactgaaag accaccgttg gctctatggg cctgcacagc
     7201 cgatacggcg agcggcggag agcccattaa atcgaagcac actgcaagat catgcgggcc
     7261 ataatcccag agcgctgagt acgaacggaa cggtcctggc ccacctccta cgctttcgat
     7321 cgaacaaacg ccttcgtcga gcgctttaga gcgcagttgc tcgtaggctg ggttaaataa
     7381 gtgaatgtgg tcgaccatga ccgccgcgtt ggccgcggaa atgaccgcat ggatttgctc
     7441 agtttcgttt aaacttagtg caaggggttt ttctacaaat actggaaccc gctttgctaa
     7501 accttgtctt aaaagatctg catgaacttc cggcggtgtg gcgactacca gaccatcgta
     7561 gggcactgtg atcgttttaa gatcacccgc aacttgggta gacgtaggca ccaactttgg
     7621 tgtttcagaa ttacgactga ccacaccggc gagttcgtaa tctggtgcgc caagaatatt
     7681 tttaatgaga atgcgtcccc atgggcccgc accggccaca acaacacgga acattctaga
     7741 tcttcgtata ggtgtcgaaa gcccagcgga aaaatcgcgt taaaccgtcg tcaagtgaga
     7801 cagtcggttc gtatcccacc ccgcggcggg ctttggaaat atcagggcac cggcgcatgg
     7861 gttcatcgcc tgggtaacta tctggataag gccttaagtc gaatttcaca ggccgcttca
     7921 aaatagtggt tagtttctcg caaagttgga gcatcgaaat ttcgggtgtt gggttcccaa
     7981 tattgtaggg ctcaccgctt tctccgagtg cgagtactcg taaaaaac
//
LOCUS       DAOMXO010000018         8022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_4714_length_8022_cov_4.915276, whole genome shotgun sequence.
ACCESSION   DAOMXO010000018 DAOMXO010000000
VERSION     DAOMXO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4714_length_8022_cov_4.915276"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLH57_00800"
     CDS             <1..698
                     /locus_tag="PLH57_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01176.1"
                     /translation="KEREQLNNEINERVGIWINDGAGKRLRKRLEKAGVKFKRKIDIN
                     GQLARAPSSADHYQSDVYNWYFEISDPENLAIAVVLPTGGAKANLSSVYFGLNVDGVG
                     SVQQVTATWLGLDPTSKNMPVTPIVGSAARMLHDKIASVSEESYIEARELDFRRSVFQ
                     VAMGYLVDPEVQDACAAVKGLQANASHGQAETRVATIVADDPLPREAPTSPSDADASV
                     RSLFDDSESQRAL"
     gene            757..1431
                     /locus_tag="PLH57_00805"
     CDS             757..1431
                     /locus_tag="PLH57_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01177.1"
                     /translation="MFGRLLITFLISLLCTGCPAFDEYAPFAPGGSYTMVASPKVELK
                     AIFFDGGIRLAPGFEYKPHWNESEGPSQHPEITLNYRSPEFELEFGGETVILSDDTTN
                     PGRQAQTLIRHSEAVVDEGCVERSAELLTLEITDREGDELRLVTEIYRTFSFARVQTG
                     ETTCSSWLREWSDALFAVSPVSLIEDWLERVFVASTILRREEAARLIILQLKFTEIGT
                     RAASAL"
     gene            1631..2044
                     /gene="flgB"
                     /locus_tag="PLH57_00810"
     CDS             1631..2044
                     /gene="flgB"
                     /locus_tag="PLH57_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRK01178.1"
                     /translation="MADYLFDSTIGGLQAALNMRLANQTVIASNIANADTPNYKSQKM
                     EFEDALRNALEISGRLQMEASSPEHFSKMDPGRIEPFIYDNPNGVVNLDGNSVDRNAE
                     QVAMAENQIQYDTATEALRRKIGLLKYAINEGGGR"
     gene            2044..2481
                     /gene="flgC"
                     /locus_tag="PLH57_00815"
     CDS             2044..2481
                     /gene="flgC"
                     /locus_tag="PLH57_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRK01179.1"
                     /translation="MDFFSSMRISASGMNAQQTRMNTISSNIANAETTETEAGGPYRR
                     KDPVFTAVADRENFGEILAGKLDEHAQGVIVTEVHEDEKPARLVYNPKHPQANADGYV
                     AMPNVNPVEEMANMIAATRSYEANATALNAAKSMAIKALDIGK"
     gene            2512..2862
                     /gene="fliE"
                     /locus_tag="PLH57_00820"
     CDS             2512..2862
                     /gene="fliE"
                     /locus_tag="PLH57_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRK01180.1"
                     /translation="MSMTIERIGQFVKNGDVESIMDQGRNIERPTNPSIGAEAPTPGA
                     HSFSEMLHKSFEKVNEIQNQADTAVKEMVAGRNKNIHETMLLMEKADMSFRMLMQVRN
                     KVLEAYREVMRMQV"
     gene            2889..4598
                     /gene="fliF"
                     /locus_tag="PLH57_00825"
     CDS             2889..4598
                     /gene="fliF"
                     /locus_tag="PLH57_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRK01181.1"
                     /translation="MFEFAKKVSTQLKDFFKTLSGGRKVGLITTAIAVGTALVALLYW
                     TGDKSYQVLQTNLATEDATATMRILREKKIPFKVEDDGKTIKIPPEAVYDLRLELASM
                     GLPQTGVVGYEVFDKQNFGTTSFVQRVNQKRALEGELMRTINNLKGVKRTRVHLAIPN
                     KSTFSEDQKAPKASVVLDIEPGIQVTDKQISGISHMIANAVEGMEIGDVTIVDANGKM
                     LSKNTNDPIARQTATLLEFQQKQESEYENRIVDILTRVVGEGRVIAKVALDMDFTQQS
                     EMQTQYDAEGAAVKSQQKENHVMDGSRPVPMGAPGAAANTPGVAPPTTPEVRSNTQKV
                     FETTNFAVPEKVVRSSKPVGSVKKMSVAVLLDGQYIKDPEKGAEAPAQFQKWSDEKLA
                     EFKTIVASSVGLDPKRGDIIEIKNMEFRRQDLEDADAQIAAYERRKMLGNVVQFAVIG
                     LIIAMFFVFVVRPFVKWLTDNTVENMESFLPKTIEELEKMQDEDEEIHEVEEAIPVIV
                     DKVDPEKVEGEMIKEKVVTLIEGNPHKAAMILHEWVHAPSKTAAAANEASAAGGKGAK
                     KPA"
     gene            4636..5667
                     /gene="fliG"
                     /locus_tag="PLH57_00830"
     CDS             4636..5667
                     /gene="fliG"
                     /locus_tag="PLH57_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRK01182.1"
                     /translation="MAETTEKVSQGYDDMPGLDKVALLLNILGTQVTAQIFKKMKDND
                     IKRIVASMGSLQKAPINVVRQVLEDFYAQISEEEEIIFGQGSGRDFILATLGEERART
                     VLGQLSVIEGSRTLEALELVDARTLSNFLINEHPQTIALILAHLDSNKKTDVLKRLPE
                     AIQTEVVLRIANLDFISPTLIAQVDEVLKQELATLGSIDTQQLGGVQPIADMLNVMDK
                     TTEQNIMARVEEKDPQLAEEIRKLMFVFEDIVYIDDRGMQQLLKEVPNDKLVVALKTA
                     PDEIKDKIFRNMSKRAGQLLKDDLEAMGPVRLSDVETAQQEIVNVAKRLEAEGKIMIS
                     RGGEADALV"
     gene            5691..6518
                     /locus_tag="PLH57_00835"
     CDS             5691..6518
                     /locus_tag="PLH57_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HRK01183.1"
                     /translation="MPGKLDFKIITSDEVGDNAAHEGHNIVTIELPKEQTIHEFDPSH
                     LRDDRNLTYSEHKKNFRELDDQKNLRFSLNRLTRGPLSVEKEEEARIEKEVQRRTKIE
                     IERLKKAELESSQKAGFEKGRLEAKAQVMEDAQPMLQQFDRLIRSLEGAKEEIYKANE
                     DFLMRMTFRLARMVILREIKEDHDYTRRLVTQILERLGTKENIKIFVGPEVFSAAEQL
                     KADLAQTLGQLKNISVELDAEFVNRGCRVETDFGEIDAKIEVQIQNIAKTLGASEVT"
     gene            6572..7882
                     /locus_tag="PLH57_00840"
     CDS             6572..7882
                     /locus_tag="PLH57_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HRK01184.1"
                     /translation="MILYRERGKLSKSVGLTFEGYLPGASIGSIVKIIPGTTHHSEDQ
                     VHSISAEVIGFKEKRVVLMPLDDASGLSNSSKIVLLEREATVKVGDFLLGRVLDGKGD
                     PIDDRGPLGEGIKGVEQRGIYGNPVNPLKRSRITQPLDLGVRAINGLITCGKGQRMGI
                     MAGSGVGKSVLLGMMARQTKADVNVIALIGERGREVREFLERDLGEEGLKRSVVVVAT
                     GEKSPLLRMRGAFLATTIAEYFCNRGADVLLLMDSVTRFAMAQREIGLSTGEPPASKG
                     YTPSVFSTLPKLLERAGTREGRGSITGLYTVLVEGDDMDEPIADATRSILDGHIVLSR
                     ELASRNHYPAIDVSSSVSRVMRDVIQSNHSQAAGEFRDLLATYKAHEDLINIGAYVKG
                     SNPRLDQAIAVHERMNAYLKQGVSEQSSLENAVADLSGIMRTVA"
BASE COUNT         2336 a         1721 c         2137 g         1828 t
ORIGIN      
        1 gaaaagagcg ggaacaactg aacaacgaaa ttaatgaacg tgtcggcata tggattaacg
       61 acggagcagg caaacgtcta agaaaacgcc tcgaaaaagc cggtgtgaag tttaagagga
      121 aaatagacat taacggccag ctcgctcgcg ccccaagttc ggctgatcac tatcagagcg
      181 acgtttacaa ttggtatttt gagatttcgg acccagagaa cctggcgatt gcggtggtac
      241 tgcccaccgg tggtgcgaag gccaatttaa gttcggttta ttttggatta aatgtagacg
      301 gagttggttc ggtacaacaa gtaaccgcaa catggcttgg cctggaccca acctctaaaa
      361 acatgccagt aacgcccatc gtgggatcgg cagcccgtat gcttcacgac aaaatcgcta
      421 gtgtcagcga agaaagttat atcgaagctc gggagttaga ttttcgccgg tctgtttttc
      481 aggttgccat gggttacctc gtggatcctg aagtacagga cgcgtgcgct gccgttaaag
      541 gccttcaggc caacgcttca cacggtcaag ccgaaacgag agttgctacc attgtggccg
      601 acgatcccct cccgagggaa gcccctacgt cgccgtctga tgccgatgcg tctgttaggt
      661 ccttgttcga cgacagtgaa tctcagcggg cgctgtagga gattccttta aactctgaac
      721 gttgatttga tctattgaat cggttagacg tgccggatgt ttggacggct gttgatcact
      781 tttctaattt ctttattgtg cacgggctgt cccgcgttcg atgaatacgc accatttgca
      841 ccgggcggta gctacaccat ggttgctagt ccaaaggtcg agcttaaggc catttttttc
      901 gacggtggaa ttcgattggc accagggttt gaatataaac cacattggaa tgagtccgag
      961 ggaccttcac agcaccccga aattaccttg aattatagaa gccccgagtt tgaactcgaa
     1021 ttcggaggag aaaccgtaat tcttagcgat gacactacga accctgggcg gcaggcccaa
     1081 accctgattc ggcattctga agccgtggta gacgagggtt gtgtagaacg ttcagccgag
     1141 cttctcacgc tggaaatcac cgatcgtgaa ggagacgagc taaggttagt caccgaaatc
     1201 taccgtacct ttagcttcgc ccgtgttcaa accggagaga cgacttgcag ttcctggctt
     1261 cgagagtgga gcgatgcgct gtttgccgtt agtccagtga gcttgattga agactggctg
     1321 gaacgagtgt ttgtggcttc cactattttg aggcgcgaag aggcagcacg cttgattatc
     1381 ttgcagttga aattcaccga aatcggcacc cgggcggcgt cggcgctttg acactcaaaa
     1441 atttggcacc ggaagagttc gtctgataca aagtcttcct cctcccgctc gctcgagtgg
     1501 aactttttac ccgaaatacg agaattatca tgaattctgg aagtgatttc ccagtgtttc
     1561 ggtgttttag aatgtcgctt agaaggtggc ctgacgtttg ctttatcttc tcttcggagg
     1621 attgtgtcag gtggcagact atttattcga ctcgacgatc ggcggtttgc aggctgcgct
     1681 taatatgcgt ttggctaatc aaacggtcat agcatctaat atcgcgaacg cggatactcc
     1741 gaactataag tctcagaaaa tggaattcga agacgcgcta cgtaacgcgc tcgaaatttc
     1801 cggtcgtctt cagatggagg cgagttcacc ggagcacttt tctaaaatgg accccggtcg
     1861 catcgaaccg tttatttatg acaatccaaa tggcgtggtg aatctagacg gcaactccgt
     1921 ggaccgcaac gccgaacaag tggcaatggc ggaaaaccaa attcaatatg acacagcgac
     1981 cgaggcgcta cggcgaaaaa tcggattgct gaaatacgct attaatgaag gtggaggcag
     2041 ataatggact tttttagcag catgcgcata agcgcttcag ggatgaatgc ccagcaaacg
     2101 cgaatgaata ccatttccag caatatcgct aatgctgaga ccactgaaac agaggccggg
     2161 ggaccatatc gtagaaaaga cccggtattt actgcggtgg ccgatcgcga aaactttggt
     2221 gaaattctgg caggaaaact agacgaacac gcgcaagggg taattgttac ggaggtccac
     2281 gaagacgaaa agcctgcccg tttggtttat aatccgaaac accctcaggc gaacgctgac
     2341 ggctacgtag cgatgcccaa tgtgaatcca gttgaggaga tggccaacat gatagcggcg
     2401 actcgcagct atgaagccaa tgcgacggcg ctgaatgcgg caaagagtat ggcgatcaaa
     2461 gcgttggata ttggaaagta gagtgaactc aatttttaag gggactcaaa tatgtcgatg
     2521 acaattgaac gaattggcca gtttgtaaaa aatggcgacg tagagtccat catggatcaa
     2581 gggcgcaata tcgagcgccc tacgaaccct agcattggtg cggaggcgcc aacacccggg
     2641 gcacacagct ttagcgaaat gctgcataaa agctttgaaa aggtaaacga gattcaaaat
     2701 caagcggaca ccgcggtaaa agaaatggtc gcgggacgca ataaaaatat ccatgaaacg
     2761 atgttgctca tggaaaaagc cgacatgagt ttccgaatgt taatgcaggt tcgaaataaa
     2821 gtacttgagg cgtatcgcga agtaatgcgt atgcaggttt aatttaggtt tttaagaaag
     2881 ggtgacgcgt gtttgagttc gcaaaaaagg tttctaccca attaaaggac tttttcaaaa
     2941 cgctctcggg cgggcgaaaa gtcggactaa tcacaacagc gatcgcggtg ggtaccgccc
     3001 tcgtagcgct actgtattgg acgggtgata aaagttacca ggttttgcaa actaatcttg
     3061 ctactgaaga tgcgacagct accatgcgta ttcttcggga aaagaaaatt ccatttaaag
     3121 tagaagacga tggcaaaaca attaaaattc ccccagaggc agtctatgac ctgcgattag
     3181 aactagccag catgggttta ccgcaaacag gcgtagtggg ttatgaggta ttcgataaac
     3241 aaaatttcgg gaccacgagt tttgtacagc gagtaaacca aaagcgcgcg ctcgaaggcg
     3301 agctcatgcg caccatcaat aatttaaaag gtgttaagcg tacccgggtg catctagcga
     3361 tcccgaataa gtcaactttt agtgaagacc aaaaagcccc aaaagcgagc gtcgttctag
     3421 atattgagcc aggaattcaa gtaacagaca aacagatttc cggaatttcg catatgatcg
     3481 cgaatgcggt cgaaggcatg gaaattggtg acgtcacgat cgttgatgct aatgggaaaa
     3541 tgctttcgaa aaataccaat gacccgattg ctcgccaaac tgcaacgtta ttggagtttc
     3601 agcagaaaca agagtctgag tatgaaaatc gcatcgtcga cattcttaca cgggtagttg
     3661 gtgaaggtcg cgttatcgcg aaagtcgcgc tcgatatgga ttttacgcaa caatccgaaa
     3721 tgcagactca gtacgatgcg gaaggtgccg cagttaaatc tcagcaaaaa gaaaatcatg
     3781 taatggacgg atcgcgcccg gtaccaatgg gggcgccagg tgccgcagcc aatacgccgg
     3841 gggttgctcc tccgaccaca ccagaggtgc gtagtaatac ccaaaaagtt ttcgaaacca
     3901 cgaactttgc agtgcctgaa aaagtggtga ggtcctctaa accagtgggt tcggtgaaga
     3961 aaatgtccgt ggcagtgttg ctggatgggc aatacataaa agacccagaa aaaggcgcag
     4021 aagccccagc acagttccaa aagtggagcg acgaaaaact ggcagagttt aagacgattg
     4081 tcgcgtctag cgtgggactc gatccgaaac gcggcgatat tattgaaata aaaaatatgg
     4141 agttccgacg tcaagatctt gaagacgccg acgcacagat cgcagcctac gaacggcgta
     4201 agatgttggg taacgtcgtt cagttcgccg ttatcggcct gatcattgcg atgttctttg
     4261 ttttcgtggt tcgtcctttt gtgaagtggc ttaccgataa tacggttgaa aacatggaaa
     4321 gttttctacc caaaactatt gaagagctag aaaaaatgca ggacgaggac gaagaaatcc
     4381 atgaagtaga agaggcgatt ccagtaatcg tggataaagt agatccagaa aaagttgaag
     4441 gcgaaatgat taaggaaaaa gttgttacct tgatcgaagg caatccacat aaggctgcga
     4501 tgatattgca tgaatgggta catgctcctt cgaagacggc cgctgcggca aatgaagcgt
     4561 cagccgccgg tggcaagggt gcaaagaaac cagcgtagta agtaagtgaa cgggacagtg
     4621 gactaaggag aggtcatggc cgaaactaca gaaaaagtca gtcagggata cgatgacatg
     4681 ccggggctcg ataaagttgc cctgctttta aatattctcg gtacccaagt caccgcacag
     4741 atttttaaga aaatgaaaga taacgatatc aaacgcatcg tagcgtcgat gggttcgttg
     4801 caaaaggcac ctattaacgt agttcgtcag gtgttagaag acttctacgc gcagatttcc
     4861 gaggaagagg aaattatatt cggtcagggt tccggacgag atttcatctt ggcgaccttg
     4921 ggcgaggaac gcgcgcgaac ggtattaggt cagttgtcag tgatcgaagg gtcgcgtact
     4981 cttgaagctc ttgagttagt cgacgcgcgt acgctttcaa actttcttat caacgagcac
     5041 cctcaaacta ttgcactaat cttggcgcac ttggattcca ataagaaaac cgacgtcttg
     5101 aagcgtttac cggaagccat tcagaccgag gtcgtgctgc gaatcgcaaa cctcgacttt
     5161 attagcccga cgctcattgc gcaggtggac gaggtactaa aacaagaact tgcaacactg
     5221 ggatcgatcg atacccaaca actgggtggc gtacaaccaa ttgccgatat gctcaacgtc
     5281 atggacaaaa ccaccgaaca aaacattatg gctcgagtgg aagaaaaaga tccacagttg
     5341 gccgaagaga tacgcaaact catgtttgtg ttcgaagata ttgtttatat cgacgaccgc
     5401 ggtatgcagc agttgcttaa agaggtaccg aacgacaaac tggtggttgc gctgaaaact
     5461 gcgcccgatg agattaaaga caaaatattt agaaacatgt caaaacgcgc cggtcagttg
     5521 ttgaaagacg atctggaggc catggggccc gtgcgtttga gtgacgtgga aactgcccaa
     5581 caagaaatcg taaatgtcgc gaaacgtcta gaagccgaag gtaagatcat gattagccgt
     5641 ggtggcgaag ccgacgcgtt agtgtgatcg aaattagaca aggagtctaa atgcccggaa
     5701 aattagattt taagatcatc acgtcggacg aggtcggtga taatgcggcc cacgaagggc
     5761 ataatattgt gacgatcgaa ctaccgaaag agcaaaccat tcatgagttt gatcccagtc
     5821 atctgagaga tgatcgcaat cttacgtatt cggaacataa aaaaaatttt cgggaattgg
     5881 acgatcaaaa aaatcttcgt tttagtctca atcgactcac tcgtggtccg ttgtcagtcg
     5941 aaaaagaaga agaagcccgc atcgaaaaag aggttcagcg acgaacgaaa atagaaatcg
     6001 aacggttaaa aaaagctgaa ctagagagtt cccaaaaagc cggctttgaa aaaggacgtc
     6061 ttgaagcaaa agcgcaagtg atggaagacg ctcaaccaat gctccagcaa ttcgatcgac
     6121 tcattcgaag tttagagggg gctaaggaag aaatatataa agcaaacgag gactttctaa
     6181 tgcgcatgac gtttcggttg gcgcgcatgg ttatattgcg cgagatcaaa gaagaccatg
     6241 actatactcg gcggctggtg acccaaattc tggaacgtct cggaactaaa gagaatatta
     6301 aaatatttgt ggggccggag gtattttctg ccgctgagca acttaaggct gatcttgcgc
     6361 aaactttagg acaactaaag aatatatcag tggagctaga tgcagaattt gtaaatcggg
     6421 gttgcagagt agaaacagat tttggcgaga tcgacgctaa aatcgaggta caaatacaaa
     6481 atatcgcgaa aactttgggg gcctcagaag tgacatgacg tcaacgcctt acaagatcga
     6541 tgaaaagacg tataacgatc gactagcgta gctgatactt tatcgcgagc gcggtaaact
     6601 cagtaagagc gttggtttga ccttcgaggg ttaccttccc ggggcatcta ttggtagcat
     6661 agtaaaaatt attcctggaa ccactcatca ttctgaagat caagttcact caataagtgc
     6721 agaggtcatt ggttttaagg aaaaaagagt ggtcctaatg ccgctcgatg acgcttctgg
     6781 cttgagtaac agtagtaaga tcgtactctt agaacgggaa gccaccgtaa aagttggcga
     6841 ctttctattg ggacgagtgc tcgatggtaa aggtgaccca atcgacgacc gcggaccgct
     6901 tggcgaagga atcaagggag tcgagcagcg cggaatctac gggaacccgg taaatccact
     6961 taagcgttca cgtataaccc agccattaga tttaggtgtt cgcgctatca acgggttgat
     7021 tacttgcggc aaagggcagc gcatgggaat tatggcgggc tccggcgttg gaaaaagcgt
     7081 actgcttggc atgatggctc ggcagactaa ggccgatgtt aacgttattg cgttgatcgg
     7141 agaacggggt cgcgaagttc gggaattttt agagcgcgac ctcggtgaag aaggactaaa
     7201 gcgctcggta gtagtggttg ctaccggtga gaaatccccg ctgctacgaa tgcgtggtgc
     7261 atttttggcc accactatcg cagagtattt ctgcaaccgt ggggccgacg tacttttgtt
     7321 aatggactcc gtgacacgat tcgcaatggc gcaacgggaa attggtctca gcactggcga
     7381 accgccagca agcaagggtt acacaccgtc tgtgttttca accttaccga agctattgga
     7441 gcgagcggga acgcgcgagg gccgcggaag tatcaccggg ctctacactg tgttggttga
     7501 gggcgacgac atggacgaac cgatagcaga tgccacgcgc tcgattctcg atggtcacat
     7561 agtgctgtcg cgagaactag catcgcggaa ccattatccg gcaatagatg tgagtagtag
     7621 cgtgagccgc gtcatgcgtg atgtgattca atcgaaccat tctcaggcgg cgggcgaatt
     7681 tcgcgaccta ttggcaacct ataaggcgca cgaagatctg attaatattg gtgcctacgt
     7741 caagggttca aatccgagac tagaccaagc aattgcagtt catgaacgca tgaacgctta
     7801 cctcaaacaa ggagttagcg agcagagtag tttagaaaac gcagtcgcgg atcttagtgg
     7861 aattatgagg actgtggcgt gaaaaaattt aaatttaaac tcgaagttgt actgcaggaa
     7921 cgaaagcgac aagaggatgt aaagctacgc gaactcatga ttgccgagag attacttcag
     7981 ggactagtgg atcagaaaaa agatctcgaa aaaaaattgg tt
//
LOCUS       DAOMXO010000019         7570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5077_length_7570_cov_6.168862, whole genome shotgun sequence.
ACCESSION   DAOMXO010000019 DAOMXO010000000
VERSION     DAOMXO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5077_length_7570_cov_6.168862"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="PLH57_00845"
     CDS             <1..1284
                     /locus_tag="PLH57_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01185.1"
                     /translation="DQYDTRRFILVDPLGNTVHPNTTAYWTMDGRLCDGQNGNPPYDP
                     LKPETCPFITNVRFAAYCNAGDTAGTRCSIARAFKFYYTVEMTTAKISGVAPLPRYDS
                     LINDPIYYTLGELLRMSIALPSDCDYANGYAITGFNADGTLQCGKVSAQVTPLAIHSF
                     TSSQPSCQFSVRKSGATSDVTMEFPSLLPPSQGFHYVSFGGVWMEQNTGTAMDLGKPE
                     SCTTNYDPAAYYYSIGTEHFQNSYMISQCNGSGNSSNLQCTAKNDGMFSMWLAALNTS
                     IPNDSMLRSAGMMANNMSHCNVCEIPGPLLVVHDFNEVHSDVAPNCPSGWDRLYTGRS
                     LGGFSLDANDNDAGYAAMTDLSSPGSCVRLPSGFTHHRTPFIECKHFSTGSGDSERIG
                     CSYDTGSDYSLWFTRSGGQAYQCSVCYKPAQMMVQ"
     gene            1319..3148
                     /locus_tag="PLH57_00850"
     CDS             1319..3148
                     /locus_tag="PLH57_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01186.1"
                     /translation="MSKNLTRYKGFSTLELVISAAVVSAIALGASSIISGLEKAQRNA
                     QAKKTRMEIVKFLAKNLSDPAAIMNSSEATTSDGNTNLKKCLYDDGGTADDCHQVPPG
                     MTPIGGGVIIQPPPGGGVLQGAFNFSIFPAAYGLAIGGTPPPIGGSGPIVIDPGTNPT
                     ATPSPAPTPTPTPTPVPLPVAPYFFELRDKHNNFVHSPGYMYWTINGVPCTGTNGYPA
                     YNAQSCPIRSSVGVIAACNDNINTTARCEIAKSFKFYYIVESNSATVGNTNTITQYSS
                     NASDPVIMTTEFLRKTAIYVSQNCDMNAGQIVSGVSGGGTVTCAKPPFQKTYIARHSH
                     SRSSPNCTFNVKRSGATTTVPTTFPQMFTDTRYSYLGSFMSPGVGTKIDLGKSGSCAK
                     VEADSDDRFARNQMTECSSDLSCTDQTWDDYTLWLIAGRDAFSGNNGSSASVTGVRHN
                     VSKCTECEIPGPVLVQHSMASHYAPECPTGYHRLWTGLSFGALSAKEETDQAGYSVRQ
                     DLSSPGSCFEMGEADVRPSQFLECDAESIVCRYRTNYDYAMWFSSINENTNAISFDRC
                     SVCYKEPTVTPPLNLIIVGVIATPTPTAAPIAPIGGSLILMGP"
     gene            3199..4761
                     /locus_tag="PLH57_00855"
     CDS             3199..4761
                     /locus_tag="PLH57_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01187.1"
                     /translation="MFREFLRKKSQKGMSTVQAAMVATLVASAAAATSSVMVHNKRQN
                     KIEQLRYSFNQVRTYGDMILLSRETLRVTSNFNPALVIGVDANRNFNRCFINDGDGML
                     DCAETTGLGYEGLRIRNLLNEFMIGGSFSPNRGYYEVNGKVCSSTTSPYCVTELTFFM
                     NPVCGTKTDGIVSCEQATSYDFSYRVRSLRPYYGVTLPEVVRTLNVTHAELNQTNLAG
                     IPNCDSANGYVPVGVQGDGSFRCATAPRNVNILAHHFLRNSGVMGCRFPGTMKNDGTF
                     YTKTFRDLTQTAPGLPVALPNGLRRSFGGMWINPRIGSAQDLGGSGSCINNSSFMSGF
                     YRECDASGCRAGSAGDFSLWLRKLDINMPQGTDLGSYYYDNLSECAICEIDGHVMVVH
                     DVDNSNVPPRCPAGYDRLYPGRSLASLSLDGGDTGEHWSGYSAAQDRASIGSCLAMHD
                     GTTSFPGFTGTMFDHARIPFAEGNHTSSTFRLGFRTGSDYAAYIGAHTPSTGETRPNY
                     CSVCWRAQPVEP"
     gene            complement(4792..5379)
                     /locus_tag="PLH57_00860"
     CDS             complement(4792..5379)
                     /locus_tag="PLH57_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRK01188.1"
                     /translation="MAQNFVISFLGLFVAMDIIGILPMYLGMTRTLDPRRRAKTVNQS
                     ILVAAVVAITFIFVGKFVFKVLGIAIWDFKVGGGIVLLVMSILDLIRGKRNDEHTAST
                     GVVPLAVPLITGPALITTVMLQVSLYGHLVVFLSLTANFVFAWFALFRSALITKVIGE
                     EGTDIVSKIAALFMTAIAFSMIRSGIFEAIAAAKM"
     gene            complement(5396..>7570)
                     /locus_tag="PLH57_00865"
     CDS             complement(5396..>7570)
                     /locus_tag="PLH57_00865"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRK01189.1"
                     /translation="QLYQGIYTMLYGKAGYGRPVIGFPRTVAACTAASLKSFWKKWYS
                     PRLMTLVVCGNVDPIKVEKLVARTWARGGETNNAAIRPRRRQVEWRSGAGNGFVRPKN
                     LLDVRPYDVSSVRWLAALPACSMRGADLPALDLATMILGHGDTSRLYDELFRKKGLVT
                     SVGCSLWAQSAPGALMLDVETSVEQAGGIQRAVWSEVERFCKEGPTKDEFDRARMSIE
                     TDRVYSVQSMDGYANRIGFLKNVTGNVRFDLEYLASIRELSLEDIRATAKRYLIDARD
                     NGELREFSLVPKSFDIKNITESTLVKKIDSKPNKRSSKKISQSAIERVTLGGQVELFL
                     IPRRDLPIVSVQVCAMGGLRAENPTNSGIGNILADVWEQGPAGMSSMDFSKFLENHGA
                     RMDAFSGRNSLGASATMLTSRVDDVLPLFCDLLNQPKFDAQEIERSKAVALEDIRGLA
                     DDGGRLCGRRFSELMFGDHPYALSMTGNKESVQNLDRKVIVDHYRKTFFSGKKVIAVS
                     GKFNSSDVVRVFEEKLETKKNENPGPDMKILHQSLSAARLVEEKKNREQSHVIVGYQG
                     TTIGDNARYDLRVLQTILGGQSGRLFREIRDKRGFCYTVSPMSFEGIEPGYFGVYIGC
                     DPSKRANAIDAIHTELDKVKTKKVSKAELNRAKEYIYGRNRMEMQMTASVALAVAFNA
                     LYGLPFDEHERLRENLSGVTEHTVQRLAARILTQPSVTSIVV"
BASE COUNT         1966 a         1947 c         1708 g         1949 t
ORIGIN      
        1 gatcagtacg acactcggag atttatattg gtagaccctt tgggaaacac cgtgcacccc
       61 aacacaactg cgtattggac tatggacggc agattgtgcg acggccaaaa cggcaacccg
      121 ccctacgacc ccctaaaacc cgagacgtgc ccgttcatca ctaacgttcg tttcgcagcc
      181 tactgcaacg cgggggacac ggctggtacg cgttgttcga tcgctagagc ttttaagttc
      241 tattacaccg tcgaaatgac caccgcgaaa atttcaggcg tggcgccatt accacggtat
      301 gactctctga ttaatgaccc aatttattat acgctcggtg aattattacg catgtcgatc
      361 gcgcttccga gcgattgtga ctacgcaaat ggttatgcga tcacaggttt caatgccgac
      421 ggcacacttc agtgcggcaa agtgagcgcc caagtcaccc ctcttgccat tcactcgttt
      481 actagctctc aaccatcgtg ccagttttct gttcgaaagt ctggagcgac gtcagacgtt
      541 acaatggaat ttccaagctt gttaccacca agccaagggt ttcattacgt gagttttggc
      601 ggcgtgtgga tggaacaaaa caccggcacc gccatggacc taggaaaacc ggagtcttgt
      661 accacaaact acgacccggc tgcctattac tactcgatcg gtactgagca ctttcaaaac
      721 tcatacatga tttctcagtg caacggatcg ggtaattctt cgaatcttca gtgtacggca
      781 aaaaacgacg gaatgttctc gatgtggcta gctgccctga atacttctat tccaaatgac
      841 agcatgcttc gctctgccgg catgatggcg aacaacatga gccactgtaa tgtgtgcgaa
      901 ataccgggcc ccctgcttgt ggtacatgat ttcaatgaag ttcacagcga cgtcgcacct
      961 aattgccctt ctgggtggga cagactttat accggccgaa gcctaggtgg cttttcgctc
     1021 gatgcaaacg acaacgatgc agggtatgct gccatgactg acctttccag cccgggcagc
     1081 tgtgtgcgcc ttccatcggg ttttactcat caccgaacac cattcattga atgtaaacat
     1141 ttcagtacgg gcagtgggga cagcgaacgc atcggctgct cctacgacac gggatcagac
     1201 tattcactct ggtttactcg ttctggcggc caagcttacc agtgctctgt ttgttacaaa
     1261 cctgcgcaaa tgatggtgca gtagggtgaa aaagatgaag aacgttggga gcaatattat
     1321 gagtaagaat ttaactcgat acaaagggtt ctcaaccctt gagctggtaa tcagcgcggc
     1381 agtggtttct gcgattgcac ttggtgcaag ttcgattatt tccggtctgg aaaaggccca
     1441 acgcaatgct caggctaaaa aaaccagaat ggagatcgta aaatttttgg ctaaaaacct
     1501 gagtgacccg gccgcgatta tgaattcatc tgaagccacc actagcgatg gcaataccaa
     1561 ccttaaaaag tgtctttacg acgatggtgg cactgcagac gactgtcacc aagtgccgcc
     1621 aggaatgact ccaatcggcg gtggagtaat tattcaacca cctccaggtg gcggagtcct
     1681 tcagggtgcg tttaattttt caatttttcc agcagcctac gggcttgcga ttggtggcac
     1741 accacctccc atcggaggct caggccccat tgtgattgat ccgggcacga atccaaccgc
     1801 cactccctca cccgcaccga ctccgactcc tacgcctaca ccggttccgc taccggtcgc
     1861 gccttatttt tttgaattac gagataaaca caataatttc gtacacagcc ccggatacat
     1921 gtattggacg atcaatggtg tgccctgtac tggcacaaat ggttacccag cctacaatgc
     1981 gcagagctgt ccgattcgaa gttccgttgg ggtcattgcc gcttgtaacg acaatataaa
     2041 caccaccgct cgctgcgaaa tcgcaaagag ttttaaattt tactatattg tcgagtcgaa
     2101 ttcagcaacg gtcggcaata ccaataccat tactcaatat agttcaaatg catctgatcc
     2161 cgtgatcatg acaacagagt ttttaagaaa aactgcaatc tacgtgtcgc aaaactgcga
     2221 catgaacgct ggccaaatag tcagcggagt atcgggcggg ggcaccgtga cgtgtgcaaa
     2281 gcctcctttt caaaaaacct atattgcacg ccactctcac agtcgctcaa gcccaaattg
     2341 taccttcaac gtaaaacgta gtggtgctac caccaccgta cccacgactt ttccacaaat
     2401 gtttacggac acccgctaca gttatcttgg ttcgttcatg tcaccgggag ttggaacaaa
     2461 aattgacctt ggtaagagtg gctcctgtgc aaaagtcgag gcagattcag atgatcgctt
     2521 cgctcgaaat caaatgacag aatgcagttc agatttaagc tgcactgacc aaacttggga
     2581 cgactatact ctctggttga tcgcggggcg cgatgctttt agcggcaaca acggatcatc
     2641 tgcctctgta accggtgtgc gacacaatgt aagtaaatgc actgaatgcg aaattccagg
     2701 tcccgtttta gtgcaacact caatggccag tcactacgca ccggaatgcc ccactggcta
     2761 ccatcgcctt tggaccggtc ttagttttgg agcccttagt gccaaagaag aaaccgatca
     2821 agcaggttat tcagtacggc aggacctctc aagccctggc agttgttttg agatgggcga
     2881 agcagacgtt cgtccctccc agtttttaga gtgcgatgct gaatcaatcg tctgccgtta
     2941 ccgaacaaac tatgattacg cgatgtggtt ttcgtccatt aatgaaaaca ccaacgccat
     3001 tagctttgat cgatgttcgg tgtgttacaa ggaaccaact gttacgccac cgcttaattt
     3061 gatcatcgta ggtgttatag caacgccgac accaacggct gcgcccattg ctccaatcgg
     3121 gggaagcctg atcttaatgg ggccgtagtt gaatttagac tttggaaagt aagtaataga
     3181 acgtttgagg agtaaattat gtttagagag tttctgagaa aaaaatctca aaaaggtatg
     3241 agcaccgtac aagctgccat ggtcgccaca ttggtagctt ctgctgcagc cgccacctct
     3301 agcgtcatgg ttcacaataa acgacaaaat aaaatcgaac aactgcgtta ctcctttaat
     3361 caagttcgca cctacggaga tatgatcttg ctctcacgcg aaactcttcg agtaacttca
     3421 aactttaatc ctgcacttgt tataggggtt gacgccaacc gcaatttcaa taggtgcttt
     3481 attaacgatg gcgatggaat gctagactgc gccgaaacca caggcctcgg ctatgaagga
     3541 cttcgaatta gaaatttact taacgaattc atgataggtg gaagtttttc acccaatcgc
     3601 gggtattacg aagtaaacgg aaaagtatgt tcttctacca ctagcccgta ttgcgtgact
     3661 gagctcactt tttttatgaa cccggtttgc ggtacgaaaa cagatggtat tgttagctgt
     3721 gaacaagcta cttcctatga ctttagctac cgagtgcgat cgctacgtcc gtattatggc
     3781 gtaacacttc ctgaggtcgt cagaactctc aacgtaacgc acgcggaact caatcaaacc
     3841 aatctcgccg gcataccgaa ctgcgattca gcaaatggtt acgtgccagt tggggtgcaa
     3901 ggcgatggat cttttcgatg cgcaaccgct cctcgaaacg tgaatatttt ggctcaccac
     3961 tttttgagaa attccggtgt gatggggtgt agatttccag gtacaatgaa aaacgatggc
     4021 accttttata ccaaaacgtt tcgcgattta acacaaactg cgcctggttt acctgttgct
     4081 ttacccaatg gcttgcgtcg cagtttcggc ggcatgtgga taaaccccag aattgggtcg
     4141 gcccaagacc tgggcgggtc ggggtcctgc ataaataatt ctagcttcat gagcggtttt
     4201 tatcgggaat gcgacgcatc gggttgcagg gctggatccg caggcgattt ctcactctgg
     4261 ttgcgaaaat tagacataaa catgcctcaa ggcaccgacc tgggttcata ttattatgac
     4321 aatctctcgg agtgcgcgat ttgtgaaatc gacggccacg taatggtagt tcatgatgtt
     4381 gacaattcaa acgttccgcc aaggtgccct gcagggtatg accgtcttta ccccggccga
     4441 agtctggcat cgctttcact cgacgggggt gataccggcg aacactggag cgggtacagc
     4501 gctgctcaag atcgcgcttc aatcggaagt tgcctggcga tgcacgacgg aacaaccagt
     4561 tttccgggtt ttacgggaac catgtttgac cacgcgcgaa ttcctttcgc agagggcaac
     4621 cacactagct ccacctttag acttgggttt cgcacgggct ctgactatgc ggcatacatc
     4681 ggtgcccaca ccccttcaac cggtgagacg cgaccaaact actgttcggt ctgctggcgc
     4741 gcgcaacccg tggagccata acccgcgact tcttttttaa cggacgagag tttacatttt
     4801 cgcggccgct attgcttcaa aaatacctga tcgaatcatc gaaaatgcaa ttgccgtcat
     4861 gaataacgcc gcaatttttg aaactatatc ggttccttct tcgccaatca cttttgttat
     4921 caatgcactt cgaaaaagcg caaaccaggc gaatacaaaa ttcgcagtta aactaagaaa
     4981 aacaactaaa tgaccgtaaa gactgacctg taacatgacg gttgtaatta gagccggtcc
     5041 agtaatgagt ggtacagcca agggtactac tccggtcgat gcggtgtgtt cgtcgtttct
     5101 ttttccccgg atcaaatcga gaatcgacat tacgagcaac acgattccac caccgacctt
     5161 aaaatcccaa atagcgatac ctagaacttt gaatacaaac tttcctacaa aaataaatgt
     5221 aatcgctacg actgccgcca ctaggattga ttgattaaca gtcttggccc ttcttcgtgg
     5281 atccaaagtt cgagtcatgc ctaaatacat tggcaagatt ccaattatat ccatcgccac
     5341 aaataatccc aaaaaagaaa ttacgaaatt ttgcgccata aagatcctcg agactctaga
     5401 cgacgatcga cgtcacgcta ggttgagtta aaattcttgc tgctaatcgc tgtacagtat
     5461 gctcagttac accgcttaga ttttctctta gccgctcatg ttcgtcaaag ggcaagccat
     5521 aaagagcgtt aaacgcaacg gcgagagcca cgcttgccgt catttgcatt tccatacgat
     5581 ttcggccgta gatgtattct ttcgcacggt tcaattctgc cttggaaact ttcttagttt
     5641 tcaccttatc tagttcggtg tgaatagcgt caatagcgtt ggcccttttg ctgggatcgc
     5701 atccgatata gacgccgaaa tatcctggtt caatgccctc aaacgacatg ggcgaaacag
     5761 tgtaacaaaa accgcgtttg tcccgaattt ctcgaaacag tcgaccgctc tgcccaccga
     5821 gaatagtttg caataccctc aggtcataac gtgcattatc gccaatagtg gtgccctgat
     5881 agccgacgat cacgtggctt tgttcccgat tcttcttttc ctcgaccaac ctagccgcac
     5941 tcagactctg atggagaatc ttcatgtcgg gtccgggatt ttcgtttttt ttggtttcta
     6001 atttttcttc gaagacccgc accacatctg acgaattaaa ttttccgcta acagcgatca
     6061 ctttctttcc ggaaaaaaag gtttttctgt aatgatcgac aattaccttt cgatctagat
     6121 tttgcactga ctctttattg ccagtcattg acaacgcgta cgggtgatct ccgaacatga
     6181 gttcgctaaa tctgcgccca caaagacggc ctccgtcatc agctaaaccg cgaatatctt
     6241 ctagcgctac tgccttggat cgttcgattt cctgggcgtc aaatttaggt tggtttaaca
     6301 aatcacaaaa cagtggaagc acatcatcta ctcgcgaagt gagcatagtt gcgcttgcgc
     6361 ccaaactgtt tctgccggaa aaagcatcca ttcgcgcgcc gtgattttcg agaaactttg
     6421 aaaagtccat tgaactcatt cccgccggac cttgttccca aacgtctgcc aggatatttc
     6481 ctattccaga attagttggg ttttcagcgc gcagcccccc catggcgcat acctgcacac
     6541 tgactattgg caaatctcga cgaggaatca aaaaaagctc aacctgaccg cctaacgtca
     6601 ctcgttcaat ggcggactga cttatttttt tgctggatcg tttatttggt tttgaatcga
     6661 ttttttttac taaagtagac tcggtgatat tttttatatc gaacgatttc ggtaccaacg
     6721 aaaattctcg cagctccccg ttgtcacgcg cgtcgatcaa atagcgtttt gcggtggctc
     6781 gtatatcttc gagagataat tcacgaatac tcgccaggta ctcgagatca aatcgcacgt
     6841 ttccagttac gtttttaaga aatccgattc ggttggcgta gccatccatt gactgaacag
     6901 aataaactcg gtcagtttca atgctcatac gggcgcggtc gaattcgtct tttgtgggac
     6961 cttccttaca aaaacgttcc acttccgacc acactgccct ttgaattcca cccgcttgct
     7021 caaccgaggt ttcgacgtcc aacattaacg ctccaggagc actttgcgcc caaagactgc
     7081 acccaaccga cgtaaccagg cccttttttc taaacaattc gtcgtagagc ctcgaagtat
     7141 cgccatgacc caaaatcatt gtcgccaaat cgagtgcggg cagatcagcg cccctcatag
     7201 aacaggcggg gagggcggcc aaccaacgca ctgaactgac gtcatagggg cgaacatcga
     7261 gtaagttttt tggtcgcaca aagccgttac cagccccact gcgccattca acttggcgac
     7321 gtctcgggcg tatggctgca ttattggtct caccgccacg agcccacgtt cgagcaacta
     7381 atttttcaac tttgatgggg tcgacgtttc cacagacaac cagggtcatc aaccgtggag
     7441 aataccattt tttccaaaag ctttttaaag atgctgcggt acaggctgca accgtccgtg
     7501 gaaacccaat tactggacga ccgtagcccg ccttaccgta gagcatggta taaattccct
     7561 gataaagctg
//
LOCUS       DAOMXO010000020         7451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5175_length_7451_cov_4.483234, whole genome shotgun sequence.
ACCESSION   DAOMXO010000020 DAOMXO010000000
VERSION     DAOMXO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5175_length_7451_cov_4.483234"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PLH57_00870"
     CDS             <1..1057
                     /locus_tag="PLH57_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRK01190.1"
                     /translation="ALGGRGIGGAIRGALLGMPLPLCSCSVMPTAIALRRRGASVSST
                     TSFTIATPETSIDALAVTAALLPGAYLVVRPLGALVLSVFVGVVSEWFSHNAKTEFQN
                     SVREFAAGMESDDLTDVCRVCGLQGRLAESPHRHNVGTQIKAVFSYAYGTFFKDIAPW
                     LFGGLVVAALIQAFFPADLFASHWLQENPWAQVIGAVLVGIPLYSCATATTPLVAVLL
                     LKGLSPGAAIAFLLSAPATNIANVLLLKREFGGRIVFAYYAALFTFCIAFGIGFDQIW
                     NWASQNWANPLWQEPMLAQVFSPERNAHSFSLVGTISSVLLLALFAKVLLLATLRKDS
                     TDHHHGPSQAESDCCDH"
     gene            1119..2882
                     /locus_tag="PLH57_00875"
     CDS             1119..2882
                     /locus_tag="PLH57_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional trypsin-like peptidase
                     domain-containing/SEL1-like repeat protein"
                     /protein_id="HRK01191.1"
                     /translation="MKILSLLFVASLGATMASASPTGGLFPYLYDQHGRKVIEDESNY
                     SDVYWTKLNTKTGKRYTGVGVLEFTFLDDSLPENLGDINAQGCSAAFIKPSKSKSSAP
                     AYVLTAGHCYVSLSGAVAEADQVIVDEPALGRVRFMRFSDAKSYIEVRTKRWVYATLK
                     GVDIAILELDATFGELEKQGVIPLEISESQPTNGEKLTLVGQPESSVPENHRVLHEST
                     CSFAGLVSDYRTVQLGSVKRSVYVDRGRTHQCSQSGGMSGGPLINSSGKIVGVVSYGG
                     AYLQDVVDLNSLNLAQYAGYNVAADSSAVPSCFDREGKVSLTWSRCKLEKPCAAEKFS
                     DNESCQKFQCKMGSGRACFEVAEIYLETGSTKKDEEKVLKQSELACEKGDARGCYAVA
                     RVYGEKNTKKDFAGAAKYFDKACEIGLPMGCYMRGAIAVDKLDGKTGESSVSAGVDYV
                     ETSCKLGSGLACGVIGDFYTTGTHKKKDVVLGLQYFARACAHSGIDEYCLKAAALLDA
                     ERRTIQKQMNGTALEKNNYTEEMINHYLVKGCRLLNRVACDVLMDREKNHLFDSSSAS
                     AVFDKSRKYRKYIDKVELNTK"
     gene            complement(2868..3653)
                     /locus_tag="PLH57_00880"
     CDS             complement(2868..3653)
                     /locus_tag="PLH57_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRK01192.1"
                     /translation="MSTLEWFDTHAHLNYEYPVSRDEYVRLADEQGVKTILTVGADPA
                     DFPSLEEIAHQFDHVFFSVGVHPHESSKYNDQVESDILRLAKDPKCLAIGEIGLDYYY
                     DHSPQDIQARVFERQLELAVRLQKPVIIHTRDAEQNTLAILREYVAKTSLKNRPGVIH
                     CFSGTAPFAQACLEMGFFISFSGIVTFKKAEDLRTIASTVPLDRILLETDAPYLAPVP
                     FRGKTNLSSYLPETARVIANARAIPLETLSKHTVSNGKRLFGI"
     gene            complement(3650..4783)
                     /locus_tag="PLH57_00885"
     CDS             complement(3650..4783)
                     /locus_tag="PLH57_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HRK01193.1"
                     /translation="MKFHKISALLLLVASIVSAYLIYNHYQVTSATGSFHSFCSVNAS
                     VDCDLVNASRYSEYFGIPVAVAGFAFAFFAMLLHFFAARDPYFRREALVLLLPMTAAA
                     VIASIANFYISVAILKTVCLFCLTHQAISLVVFVLTILAARDFGTLRPFNRSRVLGLA
                     GLGVFLFIVTAAVSAQFETAFPFDKAEFLADFRAQPVLSVPAGDSPRMGFQGDNPKLQ
                     LVEFADFECGFCGMTARHIHRLVKAYGDQIQVVFKNYPLSNECNPAMTMPMHRNSCLA
                     AKAAICAQESGKFVEMAEKLFENQKSIANVSVLHWASEIGLNRESFETCLASAKTTDR
                     LQQDVMLGKSLDIRSTPTFFVNGKKVEGIIDERRLRVLLKEFE"
     gene            complement(4780..5529)
                     /locus_tag="PLH57_00890"
     CDS             complement(4780..5529)
                     /locus_tag="PLH57_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRK01194.1"
                     /translation="MESIFEQLKLFLQSGTLLSSFGAILLAYLGGILSSFTPCIYPMI
                     PITLGLIGGSTERSVKDGWILSTFYVLGMACVYTVLGLAASLSGQIFGSMTNTTGWYL
                     TLGVILAVSSLWMLDVIKFDPNVWIQNVSRKFSKKTHTPRPDENDASLLGAFTLGASS
                     GFIAAPCTTPVLTTILAHIATERSVGMGMLLMFSFALGLGTILVAIGTFAGAMKVLPK
                     SGRWLSHIKVLSGVLILALGMYYVFKAGTLQ"
     gene            complement(5579..6268)
                     /locus_tag="PLH57_00895"
     CDS             complement(5579..6268)
                     /locus_tag="PLH57_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRK01195.1"
                     /translation="MRSNILLSDHFFGEENAPLSNGYFRLHQVHSAEVIEVTVDSAEE
                     STRTCKADALISRARPVAVQTGDCLPVLFEASLGSGNKKIAAASHAGWRGLRAGILKN
                     TLNKMIALGATDIRAAIGPAIGPCCFEVDQSTFDLFAEQPLNAPFLYERQPATSRGHA
                     SLQAKPSTNNRWIDLAGIAHAQLVNLGIAPSQVDLIAVCTYCSGLKLASYRRATHQGY
                     KAGRQWSGIAP"
     gene            complement(6258..7262)
                     /locus_tag="PLH57_00900"
     CDS             complement(6258..7262)
                     /locus_tag="PLH57_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRK01196.1"
                     /translation="MAQTIQLEVPADKSGMRLDTFLSESREELSRSQCQNLIVAGHVS
                     LNGSVITKTGSKVSIGDLIRVDVPTKTKLDLSPVDLSLEVVFEDNDLAVINKPAGLSV
                     HPSPTENGPTMVHGLLHQLSNLSGVGGVERPGIVHRIDKGTSGILVVSKNDRAHEILA
                     KYFHDHTIERTYTTLVFGDLSSKGGEGTIASDFGRHPNDRKKMTGKFIGKKSGKTRRA
                     ITHWKVQTVFRVGNRPLSLVECKLETGRTHQIRVHLSEFGFPVVGDPVYGDHQRRAAD
                     LSKIASDVGGVLNPLSHQLLHARTLGFKHPIHGTPLHFEAPLPNTFKNVLSVLEKYAV
                     "
     gene            complement(7270..>7451)
                     /locus_tag="PLH57_00905"
     CDS             complement(7270..>7451)
                     /locus_tag="PLH57_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRK01197.1"
                     /translation="SEFTKISKNYLQAVEDEDYDSLPAAVYTRGFVFQIAKSLKIPHD
                     KASSGYMTRYSSKRK"
BASE COUNT         1890 a         1725 c         1959 g         1877 t
ORIGIN      
        1 ggccttaggt ggccggggaa tcggtggcgc aatacgcggt gcgctgctcg gcatgcctct
       61 gcccctttgt tcctgcagtg taatgccaac agcaatcgcg ttgcggcgca ggggtgcttc
      121 ggtttcatca actacttctt ttactatcgc tactcctgaa accagcattg acgcgctcgc
      181 tgtgacagct gctttgttgc ccggggccta tttagtggtt cggcccttgg gtgcgttggt
      241 gctttcggta tttgttgggg tcgtgagtga gtggttttca cacaacgcta agacagagtt
      301 tcaaaatagt gttcgggaat ttgctgcagg aatggagtcc gacgacttaa ccgatgtgtg
      361 ccgagtttgt ggactgcagg ggcgtcttgc cgaaagtccg catcgacaca acgtggggac
      421 ccaaattaaa gccgtattta gttacgccta cggtacgttc ttcaaggaca tcgcaccctg
      481 gttatttggt gggctcgtgg tggcagcgtt gattcaggcc tttttccctg ccgatttatt
      541 tgccagccat tggttacaag agaacccttg ggcgcaggtg attggcgcag tgttggttgg
      601 cataccgctt tattcttgcg cgacggcgac gaccccgctg gtcgccgtct tgctcttgaa
      661 agggctctcg cctggggcag ctatcgcgtt tctcttgagt gctcctgcga ctaacatcgc
      721 caacgttctt ttgttaaaac gcgaattcgg tggtcgaatt gtgtttgctt actatgctgc
      781 tcttttcact ttttgtatcg ccttcggtat tggatttgat cagatctgga attgggcttc
      841 ccaaaactgg gcgaatccgc tttggcaaga acccatgttg gctcaggttt tttcgccgga
      901 gcgcaacgca catagtttct ctttggtggg caccatcagt agcgtgcttc ttttggcact
      961 cttcgcaaag gtcttgttgt tggccacgct tcggaaagat tcgacagatc accaccacgg
     1021 gccctcgcaa gctgaatccg actgctgcga tcattgacta tcgcttgatt ttgccgcgct
     1081 gaatcgatag gcctcctatt cgaagagggg gctctttcat gaaaatttta tcgctgctat
     1141 tcgtagcgtc gctcggcgcg acaatggctt ctgccagccc tacgggtggg ctgtttcctt
     1201 atctttacga ccagcacggc cgcaaagtga tcgaagatga atcgaactat tcggatgttt
     1261 actggaccaa actcaacacc aaaaccggaa aacgctatac cggggtaggg gtactagagt
     1321 ttacctttct cgatgattcg ttgcccgaga atctgggcga tattaacgcg cagggttgta
     1381 gtgcggcttt tattaaacct tccaagagca aatcgtcggc acccgcttat gttctaacgg
     1441 cgggacactg ttacgtttca ttgagcgggg ctgtggccga ggcggatcaa gtcatcgtag
     1501 acgaacccgc cttgggacga gtgcggttca tgcgcttttc ggatgcgaaa agctatatcg
     1561 aagtacgaac taaacggtgg gtttacgcca ctcttaaagg cgtagacatc gcgattcttg
     1621 aactagatgc gactttcgga gaacttgaaa aacaaggcgt cattccgcta gaaatttcag
     1681 agtcccaacc aaccaatggc gaaaaactga cgctcgtcgg tcaaccggag tcgagcgttc
     1741 ccgagaatca ccgagtattg catgagtcga cctgctcatt tgccggactg gtttcagact
     1801 atcgtacggt gcagttaggt agcgtgaagc gttcggttta cgttgatcga gggcgtactc
     1861 accagtgtag tcagtcgggc ggaatgagcg gcggtccgct gataaactcc tccggtaaaa
     1921 ttgtcggtgt agtgagttat ggtggagcgt atcttcaaga cgtggtggac cttaatagtt
     1981 taaatctcgc tcaatacgcc ggctacaacg tcgcggcaga ttcctctgcc gtgcctagtt
     2041 gtttcgaccg cgaaggcaaa gttagtctta cgtggtcgcg ctgtaagttg gaaaaaccat
     2101 gtgctgcgga aaagttttcg gacaatgaga gttgccaaaa attccagtgc aagatgggta
     2161 gcggtcgagc atgttttgaa gtggccgaga tttatcttga aactggcagc accaaaaaag
     2221 acgaagagaa agtgctaaaa caaagcgagc ttgcatgtga aaaaggcgat gcaagaggtt
     2281 gctacgccgt cgctcgcgtc tacggtgaaa aaaatactaa aaaagacttc gccggtgctg
     2341 ccaagtattt cgataaggcc tgcgaaatag ggcttcctat gggttgttac atgcgaggcg
     2401 ccattgcggt tgataagtta gatggaaaaa ccggcgaatc tagtgtttcg gccggtgtag
     2461 attatgtgga aacgagttgc aagctaggaa gcggtcttgc ttgtggtgtg atcggcgatt
     2521 tttacacgac cggcacccac aagaaaaaag atgttgtact cgggctacag tatttcgcgc
     2581 gagcttgcgc tcattctgga attgacgaat attgtctgaa ggccgctgcg cttttggacg
     2641 cggaacgaag aactattcag aaacaaatga atggaaccgc tctcgaaaaa aataactata
     2701 ctgaagagat gattaatcac tatttggtca aagggtgtcg tctgttgaat cgagtggcat
     2761 gcgatgtgtt aatggatcgc gaaaaaaacc atttattcga ttcgtcgtcg gcctcggctg
     2821 ttttcgataa atcgcgcaag tatcgcaagt atattgataa ggtcgaatta aataccaaat
     2881 agacgcttgc cgtttgagac cgtgtgttta gaaagcgttt cgagtggaat cgctcgcgcg
     2941 ttggcgatta ctcgagcggt ttccggcaaa taggacgata aattggtttt ccctcgaaac
     3001 ggcaccggag cgaggtatgg cgcgtccgtt tcgagtaaaa tgcggtcaag aggaacagtt
     3061 gacgcgatag tacgtaagtc ttcggctttt ttgaaggtaa cgattcccga gaatgaaata
     3121 aaaaaaccca tttccagaca tgcttgcgca aagggcgcgg tacctgaaaa acaatgaata
     3181 acaccaggtc gattttttaa agaggtcttg gctacatatt ctcgcaaaat ggcgagagta
     3241 ttttgctcag cgtctctggt gtgaataatc acaggttttt ggagtcgaac tgcaagttca
     3301 agctgtcgtt caaaaactct ggcctgaatg tcctgcggcg agtgatcata ataatagtcg
     3361 agtccgattt caccaatggc gagacactta ggatctttgg ctagtcttag aatatcgctt
     3421 tcaacttgat cgttgtattt ggacgattca tgagggtgca ctccgaccga aaagaaaacg
     3481 tggtcgaatt gatgagcgat ttcttcaagg ctggggaagt cggcgggatc agctccaacc
     3541 gttaaaatag tcttaacacc ttgctcatcg gccagtcgca cgtactcatc gcgcgaaaca
     3601 gggtattcgt agtttaagtg agcgtgagtg tcgaaccatt caagggtact cactcaaatt
     3661 ccttaagcaa gacacgcaag cgccgttcgt cgataatgcc ttctaccttt ttcccgttta
     3721 cgaaaaaagt aggagtcgat cgaatatcaa gactcttacc caacatgaca tcttgttgca
     3781 ggcgatccgt tgttttcgca gatgccaaac aggtctcgaa ggattcgcga ttaagtccaa
     3841 tttcggaagc ccaatgcagt acacttacgt tggcgatgct tttttgattt tcgaagagct
     3901 tctctgccat ttctacgaac ttgccgcttt cttgggcgca aatagcggct ttggccgcca
     3961 aacaggaatt tcggtgcatg ggcatggtca tagcgggatt gcattcgttg gaaaggggat
     4021 agtttttaaa gaccacttga atttgatcgc cgtaggcttt tacgagccgg tggatgtgcc
     4081 tagcggtcat gccacaaaaa ccacattcga aatcagcgaa ttctactagc tgaagttttg
     4141 gattatcgcc ctgaaacccc atgcgcggag aatcccccgc gggcacactc agaacaggtt
     4201 gagcgcgaaa atccgctagg aactcagcct tgtcgaacgg aaaagcggtt tcaaactgtg
     4261 ccgataccgc cgcggttacg atgaaaagaa aaacaccaag accagcgaga ccaagaacgc
     4321 gactgcgatt gaaagggcgt agagttccga aatcacgggc cgccaaaatc gtaagcacga
     4381 aaacaaccaa agagatagcc tggtgtgtaa gacaaaacaa gcaaaccgtt tttaagatag
     4441 cgacgctaat ataaaaattt gcaatcgaag cgatgaccgc agccgccgtc atcggaagta
     4501 aaagaacgag cgcctcgcgt ctgaaatacg gatcgcgagc tgcaaaaaaa tgaagcaaca
     4561 ttgcaaaaaa tgcaaaggcg aaaccggcaa cagccactgg aattccaaag tattcagaat
     4621 accgagaggc gttgactaag tcacagtcga ccgatgcgtt gactgaacaa aaactgtgaa
     4681 agctacccgt ggcgctagtc acttgatagt gattgtaaat gagatatgcc gacacgatgc
     4741 ttgcgaccag aagtagtaac gcactaatct tatgaaactt cattgcagag tccccgcttt
     4801 aaaaacgtaa tacataccga gtgccaaaat taaaacgccc gacaagactt tgatatggga
     4861 aagccaacgt cccgacttag gtaacacctt cattgcgccg gcgaaagttc cgatagccac
     4921 taaaatggtt cccaggccca gagcaaacga aaacatgagg agcattccca tgccgaccga
     4981 acgctcggtg gcaatgtggg ctaggattgt ggttaggacg ggagtggtgc aaggggccgc
     5041 gataaaccca ctcgaagcgc cgagagtgaa agctccaagg agactcgcgt cgttttcgtc
     5101 gggccgcgga gtgtgagttt ttttactgaa ctttcgcgaa acattttgaa tccagacgtt
     5161 gggatcaaac ttaatgacat cgagcatcca caaacttgaa actgcgagaa ttacgcctag
     5221 agtgagatac cagccggtag tattcgtcat acttccgaag atttgacccg agagactcgc
     5281 agcgaggcct agtaccgtgt aaacgcaggc cattcctaac acgtaaaagg tactcaaaat
     5341 ccaaccgtct ttgaccgaac gttcagtgga cccaccaatg agtccgagtg tgatcggaat
     5401 cattggataa atgcaggggg taaaactaga aagaattccg cccagatagg ctagtaagat
     5461 cgcgccaaac gaagaaagca gcgttccgga ttgcaggaat aattttagtt gttcgaagat
     5521 ggattccata gtggttccgg ttgttttagt gttggctaaa aactaccaca atcaggagct
     5581 aaggtgcaat tccggaccac tgacgcccgg ctttgtagcc ctggtgggtc gcgcgtcggt
     5641 aacttgcgag ttttaaaccc gaacagtagg tacaaaccgc aataagatcc acctgcgacg
     5701 gcgcaattcc gaggtttacg agctgagcat gtgcaatgcc tgccaaatca atccagcgat
     5761 tattggttga aggtttagcc tgaagcgaag catgccctcg tgaggttgca ggttggcgtt
     5821 cgtacaggaa gggcgcgttc agcggttgtt ctgcaaagag atcaaacgtt gattgatcca
     5881 cttcgaaaca gcaaggacca atggccggtc cgatggcggc acggatatcg gtggcgccca
     5941 gcgcgatcat tttatttaga gtgtttttaa gtattccagc tcgaagtcct cgccacccgg
     6001 cgtgggacgc ggcggcgatc tttttgttcc cacttcctaa tgacgcctca aacagcactg
     6061 gcaaacagtc gcctgtttgg accgccacgg gtcgcgcacg ggagatgagt gcatcggcct
     6121 tgcaggttcg cgtcgattcc tctgcggagt cgacggttac ttcaattact tcagccgaat
     6181 gaacctgatg caaacgaaaa tacccgtttg aaagcggggc gttttcttca ccaaaaaaat
     6241 ggtcggataa tagaatgtta gaccgcatat ttttctaaaa cacttagcac gtttttgaac
     6301 gtgttgggga gaggggcttc gaagtgcagg ggcgtgccgt ggatcggatg tttaaagccc
     6361 agcgtgcgag cgtgtaaaag ttgatggctg agtggattca acaccccgcc cacgtctgat
     6421 gcgattttgg atagatctgc tgcccgccgt tggtggtctc cgtaaaccgg atctcccact
     6481 accggaaatc cgaattccga aagatgcact cttatctggt gagtgcgccc tgtttcgagc
     6541 ttgcattcga cgagcgacag ggggcgatta cctactctga aaactgtttg taccttccag
     6601 tgggtgatag cgcgtcgggt ttttccagat ttttttccga taaatttgcc ggtcattttt
     6661 tttcgatcat tggggtggcg tccgaagtcg cttgcaatcg taccctcgcc gcccttactt
     6721 gaaaggtcac cgaacaccaa tgtcgtgtag gtcctctcga tcgtatgatc atgaaaatat
     6781 ttggcaagaa tttcgtgagc gcgatcgttt ttggacacca ccagaattcc ggacgtaccc
     6841 ttgtcgattc ggtgtactat tccgggccgt tctacgcctc caacgccgga aagattgctt
     6901 agctgatgca aaagtccgtg caccatggtg gggccgtttt ccgttggcga cgggtgaacc
     6961 gataacccag caggcttatt gatcaccgcg aggtcgttgt cttcgaacac cacttcgagc
     7021 gacagatcca ctggtgatag gtcgagtttg gttttagtag gcacatcaac ccgaattaaa
     7081 tcaccaatac ttacttttga tcctgtctta gtaatgacgc taccattcaa tgaaacgtgg
     7141 cccgcgacaa ttaagttttg gcactgcgaa cgagaaagct cttcgcgaga ctcggacaaa
     7201 aaagtatcga gccgcattcc cgatttatca gcgggaactt cgagttgaat tgtctgtgcc
     7261 atcgattcac tactttcgct tagaactata gcgggtcatg tagccggaag atgccttgtc
     7321 atgcgggatt tttaaagatt ttgcaatttg aaaaacaaaa cctcgggtgt aaaccgccgc
     7381 aggaagggaa tcgtagtctt cgtcttcgac ggcctgtagg tagtttttac taatctttgt
     7441 gaattctgaa a
//
LOCUS       DAOMXO010000021         7446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5179_length_7446_cov_4.848329, whole genome shotgun sequence.
ACCESSION   DAOMXO010000021 DAOMXO010000000
VERSION     DAOMXO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5179_length_7446_cov_4.848329"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLH57_00910"
     CDS             <1..1107
                     /locus_tag="PLH57_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRK01198.1"
                     /translation="TRFSSDMLESDFSKLRNKRSLETGAGFWGKKVDGVFERYLTPTL
                     VASADPTETSAIVRELRAAHETLGTRSPISEIRVVEDFLPKNQSEKIKVIEAIRKQLS
                     PRVLKAVSKKDRELIQEFIPDVVPAPLAVKDLPEALITHFRELDGTIGRLVHVYPRLQ
                     QGNFWDGKEVIRFAEVIRKAVADAKVGAIIAGQPPLSADMITSISKDGPKATLFAFAA
                     VLVLVLVIFPRVGVATQVIGALLLGVTWMVAVMVLRGYKINFLNFIALPITFGIGVDY
                     AVNVYSRYRQDGSRAIVSAIRHTGGAVVLCSLTTIIGYGSLLIAGSQAFVSFGELAVL
                     GELTCLVAAMIAVPSLLAVFEKRKKAKLQSNAAL"
     gene            1137..1601
                     /locus_tag="PLH57_00915"
     CDS             1137..1601
                     /locus_tag="PLH57_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01199.1"
                     /translation="MDQSARNRRSLSNFWINPRFQGRYVSWVAFSGAMLLVTNVTIFY
                     VFTSANYDLLASMLPISDTAREAMENGLVKISVLLVATGVLNLLFLIILAIALSHSAA
                     GAIYNLKQTFTAIRLGDRSARVKLRPGDDFHDVANEFNALMDRVFESKNEKS"
     gene            complement(1582..3114)
                     /locus_tag="PLH57_00920"
     CDS             complement(1582..3114)
                     /locus_tag="PLH57_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01200.1"
                     /translation="MRRFQALCLFMFLALTPAKARAEDSAPTQNADAQRGFSEPSAAE
                     KRNRQKKIEYMRLGKRVLVQWPKVPLAIQYRVELSNSQYFGRIVSEHNVTDTAFETEP
                     LRPGRYYYRVHAYSQHGLLKTLDVKDFLVEEPESIFDTFDSTGSAKKRLTPPVLMTPD
                     EGAVFPLESRIRLSWVPQENARSYRVRLWYVENTVPDSVVPWHSHQAHPIWTTEVQNP
                     WIVVRDVPYSSFIAMKAGLYRWDVSSIHERTGTPSTPAVGHFEISKYPADFIAETVLR
                     PFIRVDPFFVYQTRSNVTGLRTQDRYFATNGGVEATFWTSPHWGLNAQATVSNFTARF
                     SNSSGAPATKVVFFNTDVTMLYRTRFSPYPDEWRFTTGLGIGLHEFPQVDKVLSFNRS
                     VSVARPKSLGLAALLKLQHAISKSMAVVVQNRLLIPFTTLSLPAESRTDFSPNLTMYA
                     GIQNQKTCKVCFGLGIFGQYRSVAFEAKPDAVTRLVVFPNQPSFVRLISVGVEGSTTF
                     RF"
     gene            3100..4518
                     /locus_tag="PLH57_00925"
     CDS             3100..4518
                     /locus_tag="PLH57_00925"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRK01201.1"
                     /translation="MKSSHISRLIMGVNPVRIYGFLFFCAVTLINPLGSRRSEIWVDP
                     KLAVLVAIGVFQFFWIAMVVRRKKSEFWSLFEQVFNQHKWLWLTWIAALVVGTLSTIL
                     SPFPERSFWAHPELRDGLFFWAVLLLVAVGASFVARFNSQAVRSAEAGVVAGVALHAL
                     VMIVQVIDWRFDFTATSFVTNSVYSEMLASTVWKSQMPIGLYLHRAQASYMVGLTALC
                     LLIYLPRITASKKEKPVLVFIGLLICVLILGNSRGVLIPFALAFLLILWQKLQSFGVN
                     LRKQVWKRAAVLGLAVILFATTIGIVFQLKRPIVHESAGKINVISGGRWEYWQNSLKL
                     VRDRPVLGWGFNGYGVGAPTLERCARSAGNKVTEVLDYNFVCHMADGSVRVFHSFAAK
                     AHNWVLDQIVAFGLDGAIAWFLFIGSLFAALMRQGLAALALYAFIYLLFWYDTSMTTP
                     WLFLLAATRPSGAAIGAVDISK"
     gene            complement(4459..5691)
                     /locus_tag="PLH57_00930"
     CDS             complement(4459..5691)
                     /locus_tag="PLH57_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01202.1"
                     /translation="MGPDLEIHKPLQIRDCSYRGKPGKAVLIMFKNNGSAAAENARAE
                     LSSAGGAPETLALGSVPAKQGFGQPKQIGPCFDKDARISVKLIAQNDTNSMNNEAGAN
                     IVVGGKTSPVAIANPGAVMNTASPGIKNSIGISPIASPPTTVVDPTRGPNALSASGLS
                     VDLGVCLYHGLTGNISRCANKLSDAKVRLTNITNQPITFVNASGTPSSLHLVAGEALV
                     EPFFIVGPGHDKKVLNPGESDEFYYDARGAAGGVGTLSVAGNIYFKVGNGSQIFSKKI
                     SRSLNKVKQGYLDWYDVAGPIAFNVAPSQSQTKTVTIKNFGNTGSIVSTQVVPADPRI
                     VVLQSCTGPLSGNSQCTISIRFDQSANPTPYAGKLRVSMDQGHPYVSFSTTMDIEKDL
                     FGYINCPNGGTAGTGGCQ"
     gene            5747..5944
                     /locus_tag="PLH57_00935"
     CDS             5747..5944
                     /locus_tag="PLH57_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01203.1"
                     /translation="MAVGTPTEGAFTGLEGADAGASTVVGAVTDVVVEVEGAVRVFVA
                     VLVSVVDELPVGAIAVLETLV"
     gene            complement(6108..6506)
                     /locus_tag="PLH57_00940"
     CDS             complement(6108..6506)
                     /locus_tag="PLH57_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01204.1"
                     /translation="MRVWQSFQWPKFPSYLLPGSLMRLSLVAAFIWLTTPAFAIDCSR
                     CFDLLAEARGVEAETTRTNDLLESNKRALASINPRDVSKKVKISSNILVLSTRVETMA
                     NKRQILEQQLVQAACNGCPRPASVQNPNNN"
     gene            6576..>7446
                     /locus_tag="PLH57_00945"
     CDS             6576..>7446
                     /locus_tag="PLH57_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRK01205.1"
                     /translation="MTYFGLVIFLLSTSVLHASPTRGLASLELLTVEKAVAKALQRSP
                     TLLDRKLDQESREITYENAWKRFYLPNVSLGAAASSALSVGQMPGSATSVTGRTALDR
                     GTPASAIAVSVGEYTLYNFGRDKDIYELAKKNYERSGQTMKETERAVRFDTIRKFFSV
                     RTTQDLLDVAQRSVESAEAIYELVKSRVPLGKATSTDLSSAEVDLINSKNALIQAEIS
                     ARQTLWDLNRAMGEPIDRTYKIHADLKAKKLRMTLEEAVAMYKENSPAIKDAKLALES
                     AKLNLRYVEKSLLP"
BASE COUNT         1782 a         1676 c         1968 g         2020 t
ORIGIN      
        1 actcgattct cgagtgacat gttggagagt gatttttcta aacttcgaaa taagagatcg
       61 cttgaaactg gagcagggtt ttgggggaaa aaagtagacg gtgtttttga acgctatcta
      121 actcccactc ttgttgctag tgctgatccg actgaaacgt cggctattgt acgagagttg
      181 cgcgcagctc acgagacact agggacgcgg tcccccattt ccgagattcg ggtagttgaa
      241 gattttttac ctaagaatca gagtgaaaaa atcaaagtta tcgaagctat tcgcaagcag
      301 ctttcgccgc gcgtgttaaa ggccgtgagt aagaaagacc gtgagctgat ccaagagttc
      361 attcctgatg tagtacccgc gccgttagcg gtaaaagatc tacctgaagc attaattact
      421 cactttcgtg aattagatgg aaccataggt cgactggtgc acgtgtaccc gcgcttacag
      481 caaggtaatt tttgggatgg gaaagaggtc attcgatttg ctgaagtcat tcgaaaggct
      541 gttgccgacg ctaaagtggg tgcgattatc gcgggccagc ctccgttgtc agccgatatg
      601 attacttcta tttcgaaaga tggacccaag gcgaccttgt tcgcgtttgc cgccgtttta
      661 gttttggtat tggttatttt tcctcgagtc ggggtggcca cgcaagtgat tggggcgctg
      721 ttgcttggag ttacctggat ggtcgctgtc atggtgttgc gtggttacaa gattaatttt
      781 ctgaacttca ttgcgcttcc cattaccttt ggcatcggag tagactacgc ggtcaacgtg
      841 tacagtcgtt accgacaaga tgggtcccgc gcgattgtgt ctgcgattcg tcatacggga
      901 ggagccgtcg tgttgtgttc tctcaccacg atcatcggtt atggttcact gctaatcgct
      961 ggtagccagg cgttcgttag ttttggcgag ttagcagtat tgggcgaact aacctgtttg
     1021 gttgccgcaa tgatcgcagt tccgtcattg cttgcagttt ttgaaaaaag aaaaaaagcc
     1081 aaactgcaat ccaacgccgc gttgtaaaat aactaaaatc acctacaatc ttaggcatgg
     1141 atcaatctgc acgaaataga aggtcgcttt ccaatttttg gataaatccc cgttttcagg
     1201 ggcgatacgt ttcttgggtc gctttttccg gcgcgatgct actcgtaacc aacgttacga
     1261 tattttatgt gtttacttct gcgaactacg accttcttgc gagcatgctg cctatttcag
     1321 atactgctcg tgaggcgatg gaaaacggtc tcgtaaaaat tagtgtgctt ttggtagcca
     1381 ccggcgtgtt gaatcttttg tttttgatta tcctggcaat cgcgctttcc cacagcgccg
     1441 ctggcgcgat atacaaccta aaacagactt tcactgcaat tcgattgggc gatcgatcgg
     1501 cccgagttaa actgcgaccc ggtgatgatt ttcacgacgt agcgaacgag ttcaacgctt
     1561 tgatggaccg agttttcgaa tctaaaaacg aaaagtcgta gagccttcga caccaacgct
     1621 gattaaacgc acaaacgatg gttgattggg aaaaacgacc aatcgagtta ctgcatcggg
     1681 tttggcttca aaggcgaccg agcgatattg accaaaaatc ccaaggccga aacagacttt
     1741 gcaggtcttt tggttctgaa taccagcgta cattgttagg ttaggtgaaa aatctgttcg
     1801 gctttccgcc ggaagcgaca gcgttgtaaa aggaattagc agtcggtttt gtacaacaac
     1861 agccatactt tttgaaatgg cgtgctgaag cttgagtagt gcggctagac cgagtgactt
     1921 ggggcgcgcc actgacacgg aacgattaaa cgagagtact ttatcaactt gaggaaattc
     1981 gtgtaggcca attcccaaac ccgtcgtgaa gcgccattcg tccggataag gagaaaacct
     2041 ggtacgatag agcatggtga cgtcggtgtt aaaaaagacg actttggtgg caggggcccc
     2101 cgatgagttg ctaaaacggg cggtgaagtt tgaaaccgta gcttgcgcat ttaaacccca
     2161 atggggacta gtccaaaagg tggcctcgac tccaccattg gttgcgaaat aacgatcttg
     2221 cgtacgtaga cccgtgacgt tcgaacgagt ttggtaaaca aaaaagggat ctacgcgaat
     2281 aaatggacga agcactgttt cggcgatgaa atcggctgga tatttactta tttcgaagtg
     2341 gccaactgca ggggtgctgg gggtacccgt gcgctcatga atgctgctga catcccaccg
     2401 atacaggcca gccttcattg ctataaaaga cgagtagggc acatctcgaa caactatcca
     2461 gggattttgc acttcagtcg tccaaattgg atgagcctga tgcgagtgcc agggaactac
     2521 gctatcgggc acagtgtttt ctacgtacca caagcgtact cggtaactgc gggcattttc
     2581 ttgtggcacc catgatagac gaattcgaga ctccagcgga aatacggcgc cctcgtccgg
     2641 cgtcatgaga accggcggtg tgagtcgttt ttttgcgctg ccggtgctgt cgaacgtgtc
     2701 gaaaatcgat tcgggttctt caaccaagaa atcctttacg tcgagagtct tcaaaagtcc
     2761 gtgctgagag tacgcgtgca ctcgataata ataacgcccg ggtcgaagag gctcggtctc
     2821 gaacgcagtg tcagtgacat tatgctcgct aacgatgcga ccaaaatatt gagaattact
     2881 tagttctact ctgtattgaa tcgccaaggg cactttgggc cactggacca gcacccgctt
     2941 tccgaggcgc atgtattcaa tttttttttg gcgattacgt ttttccgcgg ctgacggttc
     3001 actgaaacca cgctgggcat cggcattttg agttggcgca ctgtcttcgg cgcgagcctt
     3061 tgcgggtgtg agcgccaaaa acataaaaag gcatagtgct tgaaatcgtc tcatatctct
     3121 cgtttaatta tgggggtgaa tccagtacga atctacggtt ttctgttctt ctgcgccgtg
     3181 acgctgatca acccgctggg gagtcgtcgc agtgagatct gggtcgatcc taagcttgca
     3241 gtattggtgg cgatcggagt gtttcagttt ttttggatcg caatggtggt gcgaagaaaa
     3301 aaaagcgaat tctggtctct tttcgagcag gtgttcaacc aacataaatg gctctggcta
     3361 acgtggattg ccgcgctcgt ggttgggact ctgagtacga tattgagccc atttccggag
     3421 cgatcgtttt gggcgcaccc ggagctgcgg gacggattgt ttttctgggc agtgctgttg
     3481 ttggtagcag ttggcgccag tttcgtagca cggttcaatt cccaggcagt tagatcggcc
     3541 gaggccggag tggttgccgg ggtggcactc catgcgctgg tcatgatcgt tcaggtcata
     3601 gactggcgct ttgatttcac ggccacaagt tttgtaacaa attccgttta ctccgaaatg
     3661 ttggccagta cggtttggaa aagccaaatg cccatcgggc tatacttaca ccgagcgcaa
     3721 gctagttaca tggtaggact cacggcgttg tgtttgctga tttatttgcc gcggattacg
     3781 gcatcgaaaa aagaaaaacc ggtcttggtg tttatcgggc ttttaatctg cgtgttaatt
     3841 ttggggaaca gccgaggggt tttgatccca tttgctttgg catttctgtt gatcctttgg
     3901 cagaagctgc aaagtttcgg agtcaacctg cgtaagcaag tgtggaagcg ggccgcggta
     3961 cttggcttag cggtaattct ttttgcgact acaattggta ttgttttcca attaaagcgc
     4021 ccgattgttc atgaaagtgc cggtaagatc aatgtgattt caggtggtcg gtgggagtac
     4081 tggcaaaatt cgttaaaact tgtacgcgac cgcccagtgt tggggtgggg gttcaatggt
     4141 tacggagtcg gggctcctac tcttgagcgt tgtgccagat cggctggtaa taaagtcact
     4201 gaagtactcg actataactt cgtttgccac atggccgatg gcagcgttcg agtatttcac
     4261 agtttcgcag ccaaagcgca caactgggtg ttagaccaaa tcgttgcgtt tgggttggac
     4321 ggcgctatcg cttggttttt atttattggg agtttattcg cagcattaat gcgccaggga
     4381 cttgcggccc tggcgctata tgcgtttatc tatttgttat tttggtacga tacgtccatg
     4441 acgacgccgt ggctattttt attggcagcc acccgtccca gcggtgccgc cattggggca
     4501 gttgatatat ccaaataaat ctttttcgat gtccatggta gtcgaaaagg aaacgtaagg
     4561 gtgtccttga tccatgctca cccttaattt accagcgtac ggcgttggat ttgcgctttg
     4621 atcaaatcga atagagattg tgcattgcga attgcctgaa agtggccccg tgcaggattg
     4681 aaggactact attctaggat ccgcagggac cacctgagta cttacgatac ttccagtatt
     4741 cccaaagttc ttaatcgtta cggttttcgt ctgtgattgg ctgggtgcta cgttgaacgc
     4801 tatggggcca gcaacgtcgt accaatctag gtatccctgt tttaccttgt tcaacgagcg
     4861 ggatattttt ttcgaaaata tctgactgcc gtttccaact ttaaaataaa tattgccggc
     4921 cacagaaaga gtacccaccc cccctgctgc gcctcgtgca tcatagtaga attcatctga
     4981 ttcgcccggg ttcagtacct ttttgtcgtg ccccggacca actataaaga acggttcgac
     5041 aagtgcctcc cctgccacca aatgaagcga cgagggcgtt ccggatgcat tcacaaaagt
     5101 tattggttgg ttggttatat tagttaaacg tactttcgca tcgctgagtt tattggcgca
     5161 tcgggagatg tttccggtaa gtccatgata taaacacact ccgagatcca cggacaggcc
     5221 gctcgccgac aacgcgttgg gtccgcgggt ggggtccact actgttgtcg ggggagatgc
     5281 tataggtgaa atgcctatgc tgtttttgat tccaggcgac gccgtattca tcacagcacc
     5341 tgggtttgcg atggcaactg gtgaagtttt tccgccaacg acaatgtttg caccagcttc
     5401 gttgttcata gagtttgtat cattttgcgc aatgagtttg accgaaatcc gtgcgtcttt
     5461 atcgaagcag gggccaattt gttttggttg tccaaaccct tgtttggctg gcaccgatcc
     5521 aagcgcgaga gtttctggag cgccaccagc gctcgatagt tcggcgcggg cattttcggc
     5581 tgctgcagag ccattatttt taaacatgat taatacggct ttgccgggtt ttcctcgata
     5641 gctgcaatcg cgaatttgta ggggtttatg aatttcgaga tcggggccca tttttactgc
     5701 gccaaacggg ctaattgcgg gttttacagc atttagcgtt ggagtaatgg ccgttggaac
     5761 accgactgag ggcgctttta cggggctaga gggggccgac gctggtgcga gcacagtggt
     5821 cggcgcggtt actgacgttg tagtcgaagt cgaaggggcc gtaagggttt tcgtggccgt
     5881 gttggtcagt gttgtagatg aactgcccgt tggcgcaatc gccgtgcttg aaactttggt
     5941 ttgagctaga actggttgtg tagaatacaa aaacagacta agcgtgaaaa acgccgtggc
     6001 gaatcgtaac atttagtggt cccctttgaa tggcaaaatt gtagctattt cagacccgaa
     6061 agcaaccgtg tcaaataaac tcattcgaaa gcggacccga gacctagcta gttgttattt
     6121 ggattttgga ccgatgcggg tcttggacag ccgttgcagg ccgcctggac tagctgttgt
     6181 tctagaatct gtctcttgtt ggccatggtt tcgaccctag tgcttaacac caggatatta
     6241 gaagaaattt ttaccttttt cgaaacgtct cgcgggttaa tcgacgcgag tgctcttttg
     6301 ttcgattcga gtaggtcgtt tgtacgagta gtttcggctt cgacccctct tgcctcggca
     6361 aggagatcaa aacaccgcga acagtcgatg gcaaaggcgg gtgtggtaag ccaaataaag
     6421 gccgcgacca gagatagccg cattaggctg cccggtaaca aataactcgg aaattttggc
     6481 cactgaaaac tttgccatac tcgcatgcgg cggagtgtcc cccttatact taaaggaata
     6541 tcggtcgttt tattttaaaa gttgaggaac cgtcgttgac ctatttcggg ttggttatct
     6601 tcctgctttc taccagtgtg ctccatgcct cgcccacccg tggtctggct tcgctcgaac
     6661 tgttaacagt cgaaaaagcc gtagctaaag ccttgcaacg tagccccaca ttgctcgacc
     6721 gcaaactcga tcaagagtcg cgagaaatta cttacgaaaa tgcttggaaa agattttacc
     6781 tgccaaacgt gtctttaggg gcagccgcga gttcagcact ttcagtgggt caaatgcccg
     6841 gtagcgcaac gagtgttacg ggtcgaaccg ctctagaccg aggaacacct gcatccgcca
     6901 tagcggtgag tgtaggggaa tacacgctct ataatttcgg tcgtgacaaa gatatctacg
     6961 aactcgcaaa aaaaaattat gaacgaagtg gccagaccat gaaggaaacc gagcgggccg
     7021 ttcgatttga tacgattcgt aaattttttt ccgtaaggac cacgcaagat ctcctagacg
     7081 tcgcacaacg ttccgtagag tctgcggaag cgatttatga gttggttaaa agtcgagtgc
     7141 cgttaggtaa agccactagc accgacttat cttcagcaga agtcgatcta attaattcta
     7201 aaaacgcctt aattcaggct gaaatctccg cacgacaaac tttgtgggac cttaaccgtg
     7261 cgatggggga acccatcgat cgaacctaca aaattcacgc tgatcttaag gccaagaagt
     7321 tgcgaatgac tctcgaagaa gcagttgcga tgtataaaga gaattcccca gcaattaaag
     7381 acgccaaact tgctctcgag agtgcaaagc taaatcttcg ttatgtcgaa aaaagtttac
     7441 tgccgc
//
LOCUS       DAOMXO010000022         7411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5210_length_7411_cov_4.420609, whole genome shotgun sequence.
ACCESSION   DAOMXO010000022 DAOMXO010000000
VERSION     DAOMXO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5210_length_7411_cov_4.420609"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLH57_00950"
     CDS             complement(<1..321)
                     /locus_tag="PLH57_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRK01206.1"
                     /translation="MASQLRSRFTALYSIALLPWSSILLELDYHQAIKPLKDGVKIEV
                     AVGHSCAEPHRKFTATVARSKEARSKGLSGRTKPLSANEAMLFVFVPAGNYYGVELTF
                     TNPIA"
     gene            complement(406..3666)
                     /locus_tag="PLH57_00955"
     CDS             complement(406..3666)
                     /locus_tag="PLH57_00955"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRK01207.1"
                     /translation="MNTNLGQDVERSKSSKGSKKSKGLKSKKVPLDLGAVKKQKGLSV
                     PRLFTRPDSHPFDEITWDIRSTAITNPDGSVVFKMDNAEVPTSWSQLATDIAVSKYFR
                     KAGVPGFGRETSVRQLVKRVAHTFRVAGEKFGGYFKTKEDAQAFEMELSHLLVHQKLA
                     FNSPVWFNCGLYQEYGIEGSGGNYFWNPQTDKIEVTKNAYEHPQCSACFIQSVGDDLM
                     SIFDLVKNEARIFKYGSGTGSNFSKIRGAMEKLSGGGYSSGLMSFLEVLDRGAGATKS
                     GGTTRRAAKMVILDIDHPEIEDFIQWKMREEKKVAALIAGGYSSDFNGDAYRTVSGQN
                     SNNSVRVSDEFMDAVEKDEEWSTRLRTSGEVHKTYKAKELFGKIAEAAWACADPGLQF
                     DSTINDWHTCAGTDRINGSNPCSEYMFLDDTACNLASVNLLKFLRDDGTFDIDGYRHA
                     CRISTIAQEIAVDFSSYPTEQIAKNSHDYRPLGLGYANLGTLLMVKGIPYDSDEGRAW
                     CGALTAIMTGTSYTTSSQIAKEKGPFPGYQNNRDSMMRVMRKHRRAADQISKKHCPKD
                     LHAAAREDWDNAVALGERYGYRNAQATVLAPTGTIGLLMDCDTTGIEPDFSLVKFKKL
                     AGGGYFKIVNQSIPAALKTLGYGEAEAAAIIEYAMGTLHLVNTPAINEDSLRAKGFTE
                     DDLKKVVNALPGVFELNQAFAQHVLGEDAYRRLGVTSDMAKSMGFSLLRHLGFSAEQI
                     DKANDVICGHMTVEGAPQLKPEHLAVFDCANKCGKHGERYIAPMGHVRMMGAAQPFLS
                     GAISKTVNIPNEATIEEVGNIYLESWKLGIKAIALYRDGCKLSQPLSSSSGKKKEEKT
                     DVAAAASTVVSPELTARRRLPRKRGGFTVEAKVAGNKVYLRTGEYEDGSLGEIFIDMH
                     KEGASFRSLLNCLAISVSLGLQHGVPLEEFVDVFTFTRFEPQGMVDHPNIKSSTSVVD
                     FIFRVLGMEYLGRTDFVHVKPEIGPAKTEQNSNEVEESDAQVSLFDAERDQRGTKSFG
                     VSVGATAENASGSALLSKHLSKMMGDAPFCNQCGHTTVRNGACYKCLNCGNSMGCS"
     gene            4015..4644
                     /locus_tag="PLH57_00960"
     CDS             4015..4644
                     /locus_tag="PLH57_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HRK01208.1"
                     /translation="MKNKKLELLFPTQIWRARLKFDRAFRKDLIRECQAFRELDDHGK
                     KWSAKHYLAGYTSYSSIANLHERSSSFLLLKTAIDREVKMYADSLEFDLKSHRGRTLR
                     MSAFWINIMGRFAHHSFHLHPLSAISGTYYLAVPAKSPGLKFEDPRIAAFMGSPPRKT
                     AATRNNQQYVEIRCDEGEVLLFESWLKHEVRANADGRERISVSFNYDWA"
     gene            complement(4641..4982)
                     /locus_tag="PLH57_00965"
     CDS             complement(4641..4982)
                     /locus_tag="PLH57_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01209.1"
                     /translation="MTFNFNVVDNVNDAHFVIPLTDKSVRSPYDALWSTAWDEQIVAH
                     ELGHMMGLNDEYDQFETTLGLSTSFLASKCSAASLMCESSPGGPLLYHYYLILRRMIC
                     DNKNGYEGLSY"
     gene            complement(5073..5474)
                     /locus_tag="PLH57_00970"
     CDS             complement(5073..5474)
                     /locus_tag="PLH57_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01210.1"
                     /translation="MRNGVFVFLTSFLLACCFTQNTWSAPNIRFDELAGSLGLPATNN
                     RCVAEKEIDPWIAKTNKRICSSFALHLRTDSRQNCRPHPTDVLKAAKFGLHKIRGTTT
                     YLSVSNRPYRYDLTLNKNYEVELTINLFFFT"
     gene            complement(5521..6438)
                     /locus_tag="PLH57_00975"
     CDS             complement(5521..6438)
                     /locus_tag="PLH57_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HRK01211.1"
                     /translation="MRDLGYTQDLFVMPFDHRGSFQAKLFGIKDRQPTAEETKMITSY
                     KKIIYDGFKKALADGVPRDKAGILVDEQFCSEFAKEAVAEGLMLAMPAEKSGQDEFDF
                     EYGHQYEEHIAAFNPTFVKVLVRQNPEGDDAANRRQMTRLKRLSEYCHRTQRKFMFEL
                     LVPATEAQLKACGGSVAQYDATLRPKVMIQAMAQIQDFGIEPDVWKLEGIESAEDSRA
                     VVHQAKIGGRQYVGVIVLGRGENADKVRQWLTVAAKTPGVIGFAVGRTVFWDALKGFK
                     EGKLSREKAVAAVADNYRGFCELWMRGKK"
     gene            6620..>7411
                     /locus_tag="PLH57_00980"
     CDS             6620..>7411
                     /locus_tag="PLH57_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01212.1"
                     /translation="MSGLKLGDTTLDSGRVLYLHSLKACVVAGFVEATGGRDPKISQE
                     ARVGNIERLHRQFERWRASHNPDILIIIGELRDGDFRPGQPERTLVKLWTSLGVRMLF
                     IAPKPARTTTHVLESLGCEVHQDLIWGRYRFECGINGIPIDSKFLCVSAEPRYCIKFA
                     SGMLRTRTLGVFLRGFGQILIPSLDSKSDMKSVLRSSLARYDVFAVGHQKILPLGKVA
                     DLRAKQGLITGIKGLKLTISGATAGRKKLKGGTTWKPKTGSFEQTN"
BASE COUNT         1796 a         1784 c         1834 g         1997 t
ORIGIN      
        1 ggcgattggg ttggtgaaag tcagctcgac accgtagtag ttgcccgccg ggacaaaaac
       61 aaacaacatg gcttcgttag ctgatagtgg tttggttcgc ccgcttagcc cttttgagcg
      121 agcttctttg ctgcgagcaa cagttgcagt gaacttgcga tgaggctcgg cgcagctatg
      181 accaacggcc acttcaattt ttacaccatc ttttagaggt ttaatggctt ggtggtaatc
      241 gagttctaat aaaatggacg accagggtaa tagggcgatt gaatataaag ctgtgaagcg
      301 ggatctaagt tgagatgcca tgctcccatt attacaaaaa aaaagaggga ggcaatcggc
      361 aggttttcac ccgcgccgat tgcctccctc taaagaagga cgtttttagc tacagcccat
      421 ggaattgccg cagtttaaac acttgtagca tgcgccgttt cgtacagtgg tgtgtccaca
      481 ctggttgcaa aacggagcgt cacccatcat ttttgataag tgcttgctaa gaagcgctga
      541 tcccgaagcg ttttcagcgg ttgcacctac ggaaacgcca aatgacttgg tgccccgttg
      601 atcgcgttcg gcatcgaata gcgacacttg agcgtcagac tcttcgactt cgtttgagtt
      661 ctgttcagtt ttcgccggtc caatttcggg ttttacgtgc acgaaatcgg ttcggccaag
      721 gtattccatg ccgagtacgc ggaaaataaa atccaccacc gaagttgaac tcttaatgtt
      781 cggatggtcg accattcctt ggggttcgaa tcgcgtaaaa gtgaacacgt cgacaaactc
      841 ttcgagtggc acgccgtgtt ggagtccgag actcaccgaa atcgccagac agttaagcag
      901 gcttctaaaa gatgcgcctt ctttgtgcat gtctatgaag atttcaccga gcgacccgtc
      961 ttcgtattcc cccgttcgca agtacacctt gttgcccgca actttggctt ctacggtaaa
     1021 gccgccacgt ttgcgaggca ggcgtcgtcg tgccgttaat tctggcgaaa ctaccgtcga
     1081 agcagctgcg gcgacatcgg tcttttcttc tttctttttg cctgacgaag aagagagcgg
     1141 ctgactcaat ttacaaccgt ctctgtatag agcgatcgct ttgattccaa gtttccagga
     1201 ctcgagatag atgttgccca cctcttcgat cgtggcttcg ttaggaatat taaccgtttt
     1261 cgatatcgca ccgcttaaaa acggttgagc tgcgcccatc attcggacgt ggcccatcgg
     1321 agctatgtag cgctctccgt gtttgccgca tttgttggca cagtcgaaca ccgcaagatg
     1381 ttctggtttg agttgaggag caccttccac ggtcatgtga ccacagatga catcgttggc
     1441 tttatcgatt tgctcggccg aaaacccgag gtgtcgaagt aagctaaagc ccatcgattt
     1501 agccatgtcg gaagttacgc ctaatcgacg ataagcgtct tctcccaaaa cgtgttgggc
     1561 gaaggcttga ttcagttcaa aaacgcctgg caacgcattt actactttct tgaggtcgtc
     1621 ttctgtgaag cccttggcgc gtagcgaatc ttcattgatc gccggggtgt tcaccaaatg
     1681 gagtgtaccc atagcgtatt caattatcgc tgccgcctcg gcttcgccgt aacccaaagt
     1741 tttaagtgct gctggaatgc tttggtttac aattttaaag taacctccgc ccgccagttt
     1801 tttgaacttc acaagcgaga aatctggttc gattcctgtt gtgtcgcaat ccatgagaag
     1861 gccgatggtc ccggtaggag caagcacagt ggcttgggcg ttccggtaac cgtagcgttc
     1921 gcccaaggcg acagcattat cccaatcttc acgcgcagcc gcgtggagat ctttcggaca
     1981 atgttttttg ctaatttggt ccgccgcgcg gcgatgtttg cgcatcacgc gcatcatcga
     2041 atcgcggttg ttctgatatc ctgggaacgg ccccttctct ttggcgatct gtgacgaggt
     2101 agtgtagctc gtaccagtca tgatggcggt aagagcgccg caccatgcgc gaccttcatc
     2161 gctgtcgtac ggaatgccct tcaccatgag taaagttcca aggttggcgt aacccaaacc
     2221 taggggacga tagtcgtggc tgtttttggc aatttgttca gttggataac tcgaaaagtc
     2281 gaccgcgatt tcctgcgcta tggtcgaaat gcggcaggca tgacggtagc catcaatgtc
     2341 gaaggtgccg tcgtcgcgta aaaatttcaa gagattcacc gaggctaagt tgcaggccgt
     2401 atcgtccaga aacatatact cgctgcaagg gttcgaacca ttgatgcgat cagttccagc
     2461 acaggtgtgc cagtcgttga tcgtgctgtc gaattgcaat ccgggatccg cacaagccca
     2521 ggccgcttcg gcaattttgc cgaacagttc ctttgcttta tatgttttat gaacttcccc
     2581 gctcgtgcga aggcgagtgg accactcttc gtctttttcg acggcgtcca tgaattcgtc
     2641 gcttacccgc accgaattgt tggagttttg cccgctaacg gttcggtagg cgtcaccgtt
     2701 aaaatcactg gagtaaccgc cagcaataag agctgcgacc ttcttttcct cgcgcatttt
     2761 ccattgaata aaatcttcaa tttcagggtg gtcgatgtcg agaatcacca tttttgcggc
     2821 ccgacgagta gtgccgccgg atttagtggc acccgcgccg cggtctagca cttcgagaaa
     2881 actcatgaga ccgctcgagt aaccgccacc cgagagtttt tccatggcac cgcgaatttt
     2941 ggaaaaatta ctacctgtac cggaaccgta tttaaaaata cgagcctcgt tttttaccag
     3001 gtcgaagatt gacatgaggt cgtcgccaac cgactgaata aaacacgctg aacactgagg
     3061 gtgttcgtaa gcgttcttag tcacttcaat tttgtcggtc tgagggttcc aaaaatagtt
     3121 tccgccgctg ccttcgattc catattcctg gtacagcccg cagttaaacc aaacgggtga
     3181 attgaaggcc agcttttggt gaacaagtaa gtgagagagc tccatttcga aggcctgagc
     3241 atcttctttt gttttaaagt aaccgccgaa tttctcgccg gccacgcgaa aggtatgcgc
     3301 cacgcgtttt acaagttggc gaacgctagt ttcgcgcccg aaaccaggca cgccagcctt
     3361 tcgaaaatat ttgctcacgg caatgtccgt ggcgagttgg ctccaggatg taggcacttc
     3421 ggcgttgtcc attttaaaca caacgctgcc gtccggattg gtaattgcag tggatcggat
     3481 atcccaagtt atttcgtcaa aggggtggga gtctggacgc gtaaacaaac gcggaacgct
     3541 caggcccttt tgttttttaa cggcaccaag atccaacggg actttctttg acttgagtcc
     3601 tttggatttt tttgagcctt ttgacgattt agaacgttca acatcctgtc caagattagt
     3661 gttcatagct tctgtttttc cctccgtgat aggaccacct agactactca ggtccggcat
     3721 acttgaaaag acgacttcaa aaaatgttat actgcgtcaa atgacgcgat cggtaatggc
     3781 gcccgaaatc acagttaaaa tcactgggat ttacttcctt ggccgtaaaa caccgacctt
     3841 gaaacaaagt tagcgccgaa gtggagggtg cgcaaggaaa acactaccta tagcggttaa
     3901 aaatattttt agcgctacat attgtgtttc tgtgccttag tgttctcacg ctatttttgg
     3961 ggctctccgg atgacgctaa gcattatttc ggtattttgt gctaggatgg ccccatgaaa
     4021 aataaaaaac tcgaactact ctttcccact caaatctggc gtgctcgcct caagttcgat
     4081 cgtgcttttc gtaaagatct cattcgtgaa tgccaagctt ttcgcgagct cgacgatcat
     4141 ggaaaaaagt ggtcagccaa acactatctc gccggttaca cgtcttattc gtctatcgcg
     4201 aatttacatg agaggtcttc aagtttttta ctccttaaga cggcgatcga tcgagaagta
     4261 aaaatgtacg ccgactcatt agagttcgat ctcaagtcgc atcgtggacg cacgcttcgc
     4321 atgagtgcat tttggattaa tattatgggg cgatttgctc accattcgtt tcatttgcat
     4381 ccgttgtcgg cgatcagcgg tacctactat ttagcggtgc ctgcgaagtc gccgggatta
     4441 aaatttgaag acccacgaat tgccgcattc atgggttcgc ctcctcgaaa aacggccgcc
     4501 actcgcaaca atcaacaata cgtagagatt cgctgtgacg aaggcgaagt actgcttttc
     4561 gaaagctggc ttaaacatga ggttagggct aatgccgatg gacgagagcg aattagcgtg
     4621 agtttcaatt acgattgggc ttagtacgaa aggccctcat acccgttttt gttgtcgcag
     4681 atcatccgac gaagaattaa ataatagtgg tacaacagcg ggcctcctgg tgaactctca
     4741 cacatgaggc tggctgcgga gcacttgctg gccagaaatg aagttgataa acctaacgtg
     4801 gtttcaaact gatcatactc gtcgttaagc cccatcatgt gccccagttc gtgagctacg
     4861 atctgttcgt cccatgccgt ggaccagagg gcgtcgtaag gggatcgaac acttttatca
     4921 gtgagcggta tgacaaagtg tgcgtcgttt acattatcga cgacattgaa attgaaagtc
     4981 atattgagtg ggcttttact ggtccatagg cgctctgcgt acccgatttt ggcctcaata
     5041 tttctgacga ggtcattttg ttggcgaatt gattaggtga aaaaaaataa attaatcgtg
     5101 agttcgacct catagttttt atttaacgtg agatcgtagc ggtagggccg attactcacc
     5161 gaaagatagg tcgtagtacc gcgaatctta tgaagtccaa atttggcagc ttttaaaaca
     5221 tcagtagggt gtgggcggca gttttggcgc gaatcggttc gaaggtgtaa tgcaaacgaa
     5281 gaacaaattc gtttatttgt ttttgctatc caagggtcta tttctttttc ggcgacgcaa
     5341 cgattgttgg tagccggtag tccgagcgag ccggctagct catcgaaacg aatattggga
     5401 gccgaccaag tattttgtgt aaaacagcag gccaaaagaa acgaagttaa aaaaacaaaa
     5461 acgccgttgc gcattcaaaa tcaatggcac aacggcgttt gaatcgaaat gcgctaggcg
     5521 ttacttcttg ccgcgcatcc agagttcgca aaaaccgcga tagttgtcgg ctacagcggc
     5581 gaccgccttt tcgcgcgaaa gtttgccctc tttaaaaccc ttgagcgcgt cccaaaaaac
     5641 agtacgacct acggcgaatc caattacgcc cggagtcttt gccgctactg tcaaccactg
     5701 gcgaactttg tcagcgtttt cgccacggcc taaaacgatg actcctacgt actgccggcc
     5761 accgattttt gcctggtgaa ccacagcgcg actgtcttct gcgctttcaa taccttcaag
     5821 tttccagaca tctggctcaa taccgaaatc ttggatctga gccattgcct gaatcatgac
     5881 ttttgggcga agagttgcgt cgtattgtgc cacgctgcca ccgcatgcct taagttgtgc
     5941 ctcagtcgct ggaacgagca attcaaacat aaatttacgt tgagtgcgat ggcagtactc
     6001 actcaaacgc ttgagacggg tcatttgtcg gcgattggcg gcgtcgtctc cctctggatt
     6061 ttggcgaacc aaaactttta caaaggtcgg gttgaacgcc gcgatgtgtt cctcgtactg
     6121 atggccatat tcaaagtcga attcgtcctg gccagatttt tctgctggca tggcgagcat
     6181 aagtccctcg gccactgctt ctttggcaaa ttctgaacaa aattgttcgt caaccaaaat
     6241 accggccttg tcacgaggga caccgtcggc cagagctttt ttgaatccgt cgtaaatgat
     6301 ctttttataa gaagtgatca tttttgtttc ttctgccgtg ggttgccgat ccttgattcc
     6361 aaaaagtttg gcctgaaacg atccacggtg atcgaacggc ataacgaaca agtcctgagt
     6421 gtatccaagg tcgcgcataa aaaacccttt ctatttaaac ggtttctaaa tcttttaaac
     6481 tatttcgtaa cgatttagag cgtcttcagc aaggaatttt cgtgaagcga tgcgtctcaa
     6541 ttcggcgctg gcgattgtta gactgaatta ttctcgattg actttctcac ctattacgac
     6601 gttttataga ggcatcgtta tgagtggatt gaagttgggg gatacaacgc ttgattcagg
     6661 gcgagtgctt tacttacact ccctgaaagc ctgcgttgtt gctggatttg ttgaagccac
     6721 cggtggtcga gatccaaaga tttcgcaaga agcccgtgtt ggcaatatcg aacgtcttca
     6781 taggcagttc gagcgctggc gagcatcgca caacccagat atcctcatca taatcggaga
     6841 attaagagat ggggactttc gtcccggaca gcccgagcga acattagtta agttgtggac
     6901 gtctttaggg gtgcgaatgt tatttatcgc tcctaagccg gcgcgaacaa ccacccatgt
     6961 gctcgagagt ctagggtgcg aagttcacca agatttaatc tggggccgct accggtttga
     7021 gtgcgggatc aacggcattc ctatcgactc aaaatttctc tgtgtttcgg cagagccgag
     7081 atattgcatt aagtttgcct ctggtatgtt acgcacccgg acactcggcg tatttttgcg
     7141 tgggttcggg caaattctta ttccgtccct tgattccaag tccgatatga agtctgtatt
     7201 gcgttcgagt cttgcgcggt atgatgtttt tgcggtaggt catcagaaga tacttccttt
     7261 aggtaaggtg gccgacttga gagcaaaaca ggggctcatc acgggtataa agggtctgaa
     7321 actcacaatt tcgggagcca ccgccggaag aaaaaaacta aaaggcggaa ccacatggaa
     7381 accaaagact ggctcgttcg agcaaaccaa c
//
LOCUS       DAOMXO010000023         7209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5410_length_7209_cov_5.276908, whole genome shotgun sequence.
ACCESSION   DAOMXO010000023 DAOMXO010000000
VERSION     DAOMXO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5410_length_7209_cov_5.276908"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLH57_00985"
     CDS             complement(<1..421)
                     /locus_tag="PLH57_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01213.1"
                     /translation="MNSTLNIYGRSALLAITLISGDLFAGDAMFVYRGEAKNQGKIVY
                     IERHRVFLDQAGKIERAETEYERSDGSKIASLSSDFRSSLTSPDHTIIDLRTGEVQGV
                     RRESEKIVLFYKEKDKPEETKTLTGEEIHERVLVGCQG"
     gene            complement(418..1374)
                     /locus_tag="PLH57_00990"
     CDS             complement(418..1374)
                     /locus_tag="PLH57_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRK01214.1"
                     /translation="MSQTFNVKAAANMSALTTHTLRAWERRYGVVTPRRSANGRRLYT
                     MEDVEKLRLLGQLVEAGHSIGQLAELSPAQLRSLEKSQHSAPDRSHEVDFLEKIRRQL
                     IKSVEQLDFDEMDRLILQARISTPLREFVLQVVAPLLTQVGYLVHTRKLDVAQEHAFS
                     AVLRNHLGELLSQVQKTNDWRSNTSSALPRFLFSTPEGDLHEFGILLAAILTGSRGFK
                     FRYLGANMPAVDLGRAVLAVKAQIVVLGAVEADETRLKSPLKEYITELGRELQKEKLW
                     NPMQIKIWIGGQCDFDLKKHPYSAVLFHTRTLLEFDRQLDFL"
     gene            1504..2838
                     /locus_tag="PLH57_00995"
     CDS             1504..2838
                     /locus_tag="PLH57_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRK01215.1"
                     /translation="MKIAVTGPTGFVGRALLAHLAERGHVCTALARDASVAATLAAKA
                     VSVEAVDSYLEEIDALVHLAGEPVANHRWDDQTKKRIRDSRVQGTRQLINALARIPVS
                     KRPKTLVVASAIGYYGDRGDDELNEASTLGTGFLADVTSAWENEALFAEKLGVRVVRV
                     RLGVVLGVGGGALQKMRPVILGSGKQWMSWVHMRDVVRAIEFAVTNEKVEGVFNLVAP
                     TPVTNAEFTRAFGAATGAAVILRTPAWVLKSALGEMATMLLDSARVLPLKLRHWGFDF
                     EFSKLREALRDIYPDPLTKKLEVHQFVPRSLEQVFSFFSKAENLETITPPWLNFRITK
                     TSTPVLGENTIIDYALKIHGVPVKWRSRIESWEPERQFVDTQIKGPYKKWHHTHLFKA
                     VSGGTLVSDIVLYRVPGGKLGQLFGGAFVSNDVEKIFKYRQARIGEMFGIKK"
     gene            complement(2855..3547)
                     /locus_tag="PLH57_01000"
     CDS             complement(2855..3547)
                     /locus_tag="PLH57_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRK01216.1"
                     /translation="MKPRILAIEDAPDIQLLIRGALGASYDVVISETIEQARGALANN
                     PFDLILLDLGLPDGDGMRFFAELKATGKLANLPVFIVTGSNSIQEKTIGFQLGIEDYI
                     VKPFDPLEMKLRVEARIKKGIDNAQSANSISIGNLNIDLASHHIRIQLENETRTVELS
                     SKEFGILTFLARVPNKIKSREQIIEAVWGNGIHLSDRTIDSHVSRLRKKLLGTNVRLE
                     AVTQVGYRIALS"
     gene            3669..4079
                     /locus_tag="PLH57_01005"
     CDS             3669..4079
                     /locus_tag="PLH57_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRK01217.1"
                     /translation="MSESSYIDRSALVQFDDLDQEGLPPVLPRLIALFLKNAPKRIAE
                     FTKHAKTLSIKGLKWEAHALKSSSQTLGARKLAEACLKIESLADKADSKKIRRLVTNL
                     KKTYVLSCRELMEIKVERSKRENSSEYTDLRTVA"
     gene            4080..>7209
                     /locus_tag="PLH57_01010"
     CDS             4080..>7209
                     /locus_tag="PLH57_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRK01218.1"
                     /translation="MRTRKKNIPSSWRGEAENSEIINSYEAGLPKELEMALFRERISN
                     ATKNYVLLSRLVLKVASVVVFLLALPVLFNAHLNGGLFGVWSPMQFNTALTFVLVSVG
                     LHYLSKNRHKFTQGSGALIAVTGGLTLFQYVVGIDLGIDELFRKAHSGVYSVFLGRMA
                     PNSAISFFFLGLSFVVLGSRNRGPKRVFAGGILAALPFAFALVTLAGYILGMSTYYGW
                     GSYALMALPTAVALLTASAAILAGTLLELRATFEGRVHVVPLCFTFSGFTVSIFLWQS
                     VLVHDAKTVERVTDLQIEVAGRKIEKSLEGTALALKRMATRIEYLGARDVGYLSVDAN
                     SYIRHLPGLTRIGLVDQNYRVTWSYPNEFRHQVVNFNQASEPRRKAAFDRAKSNPSEF
                     TLSQSVELRSGGTGFLMPIALRNKGKVNGFLYATVTAQKLFESVLDDNAYNIRIGDDR
                     GLFFTKELDHGLGDKRWLRKQKITIGDSVWQFEMAPSLAILRKEVSIFHVVILLVGIA
                     LSLLIGLFLDLFFVSREQRIKIAEKDASFRLMVNGIHDYAIVFLDPEGGVVTWNTGAE
                     TIFGYPADEIVGKNLLVLCDSTDKKNETIRLFLFKAALDRDSGGQEYEGLLCDKNGRA
                     FWGQIVLRRVSLEDGKHVGFAMIARDLTTEKKLEQERQEILTHLDIALEASALGIWSL
                     DIPTGKVWRSNGHDQIFGHARALVDWDMEKFFEHVIPEDRQKVRDHLQEAYKAESSQF
                     QFRIRRADDHSIRWISIECKTYPPGQEDRTQIIGTIKDITQSKLEMEERATFSQWQRA
                     ILQGANYSIISTDVEGVIQTFNRSAEVMLGYRADEVIGKQTPKIFHELSEVQNRALVL
                     SRELGVPIEAGFDSFVAKARSLDCVDQAEWTYVRKDGTKFPVNLSVTALRNEKREVTG
                     YLGIASDITEQRKVLTELQSTTKKLKAIFDASPVGILVLDPQFNVTIWNPACEKIFGW
                     SEREVLGKPQPFVIRESENESLANLESVKKSKNPVRFQVDRITKKGTKIKIESAAMSV
                     FDDEGNIVGIVSI"
BASE COUNT         1912 a         1601 c         1811 g         1885 t
ORIGIN      
        1 atccttggca gcccaccaaa acgcgttcgt ggatctcttc gcctgtaagt gttttggttt
       61 cttcgggttt gtctttttct ttgtaaaata aaactatttt ttcagattcg cggcgaacac
      121 cctgaacttc acctgtgcgt aaatcaataa ttgtgtgatc aggcgacgta agactcgagc
      181 ggaaatccga actgagagat gcaattttgc tgccgtcgga tcgctcgtac tcggtttccg
      241 cacgttctat tttgccagcc tgatccaaaa aaacgcggtg ccgttcgata tacacgatct
      301 tgccttggtt tttggcctcc ccacgataga cgaacattgc atcgccggca aagagatcgc
      361 cggaaatgag cgtaatcgct aatagtgcag atcgaccata gatgttcaac gtagaattca
      421 tagaaaatcc agttggcgat caaactctaa tagtgtgcgg gtgtgaaaca atacggcgct
      481 gtagggatgt ttttttaaat caaaatcaca ttgacctccg atccaaattt ttatttgcat
      541 tggattccag agtttttctt tttggagttc tcgcccaagt tcggtgatgt actctttgag
      601 tggacttttt aatcgcgttt cgtcagcttc gaccgcaccg agtacgacaa tttgggcttt
      661 taccgcgaga accgcccgtc caagatcgac tgctggcata tttgccccga gataccgaaa
      721 cttaaatccg cggcttccgg ttaaaattgc ggctagtaaa attccaaatt cgtgtagatc
      781 gccttccgga gtggaaaaca aaaatcgagg gagcgcagag gaagtattgc tgcgccagtc
      841 gtttgttttt tgcacctggg agagtaattc tccaaggtga ttgcgaagta ctgctgagaa
      901 tgcatgttct tgggcgacgt caagtttacg ggtatgtacc aggtaaccaa cctgagtgag
      961 caggggagcc accacttgaa gcacaaactc gcgaagcggg gtactaatgc gagcttgtaa
     1021 gattaatcgg tccatttcat cgaagtctaa ctgttcgact gacttaatta attgccggcg
     1081 aattttttcg agaaaatcga cttcgtgcga tcgatctggt gctgagtgtt gcgacttttc
     1141 gagcgaccgc aactgagcgg ggcttaattc ggctagctgc ccgattgaat gacccgcttc
     1201 gactaactgg ccgagtagtc gcagtttttc gacgtcctcc atggtgtaga gtctacgacc
     1261 gttagcagac cgtcgcggtg tgactacgcc gtagcgccgt tcccaggctc gaagcgtgtg
     1321 ggtggtgagt gctgacatgt tggcagccgc ttttacgttg aatgtttgac tcaagggttc
     1381 cctcgcaagt tagttcccat tctaggctat ttgtctaatg tttgtcaaat taatagcttt
     1441 atatatgttt gacatatatt agacaaaaga gctacaatag ggtttgtcgg aggcgttttg
     1501 tctatgaaaa ttgcggtaac cggacctacc ggttttgttg gtcgtgcact actcgcgcat
     1561 ctcgctgaac gaggacatgt ctgcaccgcg ttggcgcgcg acgcgtcggt tgctgccact
     1621 ctggccgcga aagcggtgtc ggtcgaagct gtcgatagtt atctagaaga gatcgacgca
     1681 ctagtgcacc tagcgggtga accagtcgct aaccatcgct gggacgatca aaccaaaaaa
     1741 cgaatacgcg attcacgagt gcagggcaca aggcagctaa tcaatgcgct tgctcgcata
     1801 ccggtttcga aaaggcccaa aacgttagtt gttgcgtctg ccattggtta ttatggcgat
     1861 cgaggcgacg acgaactcaa cgaagcaagt actcttggaa ccggatttct cgccgacgtg
     1921 accagcgcct gggaaaacga agctctgttt gccgaaaaac ttggggtgcg cgtagtgcga
     1981 gtgagactgg gtgtcgtgct tggtgtcggt ggcggagcac tgcagaaaat gcgccctgtg
     2041 attctggggt cgggaaaaca gtggatgagt tgggtgcaca tgcgcgacgt cgtgcgtgct
     2101 atcgagttcg ctgtcaccaa tgaaaaggta gagggcgtat ttaacctggt ggctccgacg
     2161 cctgtaacca acgcagaatt tacgcgcgcc ttcggcgcag ctacgggagc cgcagtcatc
     2221 ctgcgaacgc cagcgtgggt tttaaagagt gccttgggcg aaatggccac aatgctgcta
     2281 gatagtgcac gcgtgttgcc gttaaagctc cgacattggg ggtttgattt cgaattttct
     2341 aaactccgcg aagcattacg ggacatttat cccgaccctc taactaaaaa actagaggtg
     2401 catcagtttg ttccccgatc gctcgaacaa gttttttcat tctttagtaa agccgaaaat
     2461 ctagaaacta ttacaccccc atggctgaac ttcagaatca cgaaaacgtc gacccccgtt
     2521 ctcggagaaa acactataat agactatgcg ctaaaaattc acggagtacc tgttaagtgg
     2581 cgatcccgta ttgaaagttg ggaacctgag cgacagttcg tggacacaca aattaaaggc
     2641 ccgtacaaga agtggcacca cactcatttg tttaaagccg tgagcggcgg cacgctcgtg
     2701 tccgatatag tgctttaccg ggttcctggc ggaaagctcg gtcagctttt tggcggtgcc
     2761 ttcgtctcaa acgacgtgga aaaaattttc aaatatcgtc aagctaggat tggcgaaatg
     2821 ttcgggatca aaaagtgata tgtcgctgag atgtttagct aagagcaatg cgatagccga
     2881 cctgcgtgac ggcttccagt cgaacattgg tgccgagaag cttttttcgc aagcgactta
     2941 catgggagtc aatcgttcgg tccgacagat gtatgccgtt gccccaaaca gcctcgataa
     3001 tttgttctct gctttttatt ttgttgggta ctcgagccaa aaacgtaagg atgccaaatt
     3061 ctttactgct aagttcaacg gtacgtgttt cattttcaag ttgaatgcga atgtgatggc
     3121 tcgccaggtc gatattcaaa tttccgatcg atatggaatt ggccgattga gcgttatcga
     3181 tccccttttt aatgcgggcc tctacccgca gtttcatttc aaggggatcg aatggtttga
     3241 caatgtaatc ttcgattcct agctgaaatc caatggtctt ctcttgtatt gagttgcttc
     3301 cggtgactat aaaaactggt aggttagcca gctttccagt ggcttttagt tctgcaaaaa
     3361 agcgcatacc gtcgccgtcc ggtagaccaa gatcaagaag aataagatcg aagggattgt
     3421 tggcaagtgc tccgcgggcc tgttcaattg tttccgaaat tactacatcg taagaggcac
     3481 caagagcccc ccgtataagt agctgaatgt ctggagcatc ttcaatggcg agtatgcggg
     3541 gcttcacagc acacttttcg tcagaacctc tggatatctt taggagtctc agatgttgca
     3601 aaaatgcaac agaaacgcca tttggccggc atagggctca agtacaattt tggaaaagcg
     3661 aggtctctat gagtgaatca tcttacatag atcggtcggc tttggtgcag ttcgacgatc
     3721 tagatcagga aggactgcca ccagttctac cgcgtctgat cgccttattt ttaaagaatg
     3781 ctcccaagag aatcgccgaa tttacaaaac acgcaaaaac tctttccatt aagggtctta
     3841 aatgggaagc gcatgcacta aaatctagct cccaaaccct cggagcccgt aaactcgcag
     3901 aggcatgtct caaaatcgag agcctcgccg ataaagccga ttcaaagaaa attcgaaggc
     3961 tcgtaactaa cttaaagaaa acctacgtat tgagctgtcg ggaattaatg gaaattaaag
     4021 tggagcgcag caaaagggaa aatagttccg agtacaccga cctgaggaca gtagcttagg
     4081 tgagaacgag aaagaaaaat attcctagtt cctggcgcgg cgaagctgaa aatagcgaaa
     4141 taataaacag ttacgaagcg ggattgccga aagaattaga gatggctcta tttagagaga
     4201 gaatttctaa tgccacaaaa aactatgttt tactctcgcg cctagtttta aaagttgcga
     4261 gtgttgtggt ttttttattg gcactgccag tgttatttaa tgcacacctc aatggtggat
     4321 tgtttggcgt ttggtcaccc atgcaattca acacggcatt gacctttgtc ttagttagtg
     4381 tcgggctaca ttacctctca aaaaaccgtc acaagttcac tcagggtagt ggcgcattga
     4441 tagcagtcac tggtggcctg acactgtttc agtatgtcgt gggcattgat ctcggaatcg
     4501 atgaactttt tcgtaaagcg cacagtgggg tatactccgt atttttaggg cgcatggccc
     4561 caaactctgc gattagtttt ttctttttag gtctgagttt cgttgtgttg ggttctcgaa
     4621 ataggggtcc aaaaagggtc tttgcaggcg gaatactagc ggcgctgccc tttgctttcg
     4681 cgctcgttac tttggccggc tacatacttg gcatgagcac ctattacggt tggggcagtt
     4741 atgcgttaat ggccctgcct acggcggtgg cattgcttac agccagtgca gctatcttgg
     4801 cgggtacgct actagagttg agagccacat tcgagggacg cgttcatgtc gttccattgt
     4861 gttttacttt ttcgggattc acggtgtcga tttttttatg gcaaagcgtg ttagttcacg
     4921 atgcaaaaac cgtggaacga gtcacagatc ttcaaattga agtggctggt cgcaaaattg
     4981 aaaagtcact tgagggaact gcgcttgccc taaaacgcat ggccactcga atcgagtatt
     5041 tgggcgccag agacgttggg tatctatcgg ttgatgccaa ttcctatatt cggcacctac
     5101 cgggccttac acgcattgga ttagtggatc agaactatag agtcacatgg tcatatccaa
     5161 acgagtttcg gcatcaggtt gtaaacttca atcaagcctc ggaaccacga aggaaagctg
     5221 cgttcgatcg tgcaaaatcg aatcctagtg aatttacctt aagccaaagc gttgaacttc
     5281 gtagtggagg aaccggtttc ctaatgccaa ttgcgctgcg caataaaggc aaggtaaatg
     5341 ggtttcttta cgctaccgtc accgcccaaa aactctttga aagcgtgtta gacgacaacg
     5401 cctataatat tcgaattggg gatgaccgcg gcctgttttt cacaaaggaa ctagaccacg
     5461 gtctgggtga caaacggtgg ttgcgtaaac agaaaattac aattggtgac tccgtatggc
     5521 agttcgaaat ggcgccatcg cttgcgatcc tgcgcaaaga ggtgtcgatc tttcatgttg
     5581 taattttgtt agtcggaatt gcgctatcgc ttttaatagg gttgttttta gacctttttt
     5641 tcgtatcccg cgaacaaaga ataaaaattg cagagaaaga cgcttccttt cgactcatgg
     5701 tcaatgggat tcacgattat gccatagtct ttctcgaccc cgaaggcgga gtcgtgacct
     5761 ggaatactgg ggccgaaacg attttcggct accccgcgga tgagatcgtc ggtaaaaatc
     5821 tattggtact gtgcgatagt accgacaaaa aaaacgaaac cattcgactt ttcctgttta
     5881 aagcagcact agaccgagac agcggaggcc aggagtacga agggttactt tgcgacaaaa
     5941 atggacgcgc cttttggggc caaattgtct tacggcgcgt tagtttagaa gatggcaaac
     6001 acgttggttt cgccatgatc gcccgggatc taaccactga aaaaaaatta gaacaagaac
     6061 gacaggaaat actgactcat ttagacattg cgctcgaagc atccgcccta ggaatctgga
     6121 gtttggatat tccgacagga aaagtgtggc gcagtaatgg gcacgaccaa atcttcggcc
     6181 atgctcgagc tctagtagac tgggatatgg aaaaattttt cgagcacgtg attccggaag
     6241 atcgccaaaa agtgcgcgat catcttcaag aagcctataa agcagaatcc tcccagttcc
     6301 agtttcggat tcgacgggct gacgaccaca gcattcgttg gatatcaata gagtgcaaaa
     6361 cctatccgcc tggccaggaa gatcgcactc agattatcgg tacgattaaa gacatcactc
     6421 aatcgaaact agaaatggaa gaacgggcta cattctcaca gtggcaacgt gcaattcttc
     6481 agggcgctaa ctattcaatt atttctaccg acgttgaagg cgttattcaa accttcaacc
     6541 ggagtgctga agtcatgctg gggtaccggg ccgacgaagt aataggtaag cagactccaa
     6601 aaatatttca tgaattgtcg gaggttcaaa atcgtgcatt ggttcttagc cgagaattgg
     6661 gtgttccgat tgaagccggt tttgactcat ttgtagcaaa ggcccgatca ctagactgtg
     6721 tcgaccaagc agagtggact tacgttcgta aagatggaac taagtttccg gtgaatctat
     6781 cggttaccgc gctccgaaac gaaaaacgag aagtaacagg ttacctcgga attgcttcgg
     6841 acattactga gcaacgaaaa gtactaaccg agctgcaaag tactaccaaa aaactaaaag
     6901 caatatttga cgcgagtcct gtaggtatat tggtattaga tcctcaattt aacgtcacca
     6961 tttggaaccc cgcgtgcgag aagatattcg gctggagcga acgcgaggtg cttggtaaac
     7021 cgcagccatt tgtaattcga gaaagcgaaa atgaatcgct cgcaaatctc gagagcgtga
     7081 aaaaatcaaa aaatccggta cgcttccagg tagaccgcat cactaagaaa ggtactaaaa
     7141 taaagatcga atctgccgca atgtcggtat tcgatgatga aggaaatatc gtagggatcg
     7201 tttcgataa
//
LOCUS       DAOMXO010000024         7182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5437_length_7182_cov_4.775782, whole genome shotgun sequence.
ACCESSION   DAOMXO010000024 DAOMXO010000000
VERSION     DAOMXO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5437_length_7182_cov_4.775782"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLH57_01015"
     CDS             complement(<1..502)
                     /locus_tag="PLH57_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRK01219.1"
                     /translation="MEKFRSAPTRPALGKGLASLLPTAAVTPPPTPPTESSLGGGATE
                     PDSNRDRHPGISMAAIDEIVPNEFQPRKIFDDITIEELSQSIKENGLIQPLIVRKAQR
                     GYQLIAGERRWRAAKIAGLKYVPIVIRRTTDREALELALIENIQREELNCFDTALAYY
                     QLIQDFE"
     gene            complement(518..1336)
                     /locus_tag="PLH57_01020"
     CDS             complement(518..1336)
                     /locus_tag="PLH57_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRK01220.1"
                     /translation="MGRVIAVTNQKGGVGKTTTTVNLAAALAVAERKTLILDLDPQGN
                     ASSALGVSKEAFAEKNLYHAIIGLVPMSQIIQPTEIDSLFVAPSNNDLTGAEIELVSS
                     FARENKLKAALAEIRNQYDYIFIDCPPSLGLLTVNALTASDSFLVPLQCEYFALEGLS
                     QLLNTVGLIRQNLNPALQDEGIVLTMYDGRNNLANEVVREVRTHFGSKVFETIIPRNV
                     RLSECSSFGKPILLYDIDSKGSVAYLSLAKELIARTMSSQQRTTTSGSPEVNTN"
     gene            complement(1488..2165)
                     /locus_tag="PLH57_01025"
     CDS             complement(1488..2165)
                     /locus_tag="PLH57_01025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK01221.1"
                     /translation="MSELVILEGAGPSLDECKQALAYASVPPVDKLAKYMQLVLAINE
                     TNNLTGAKTPKEFAVKHIADTWLAIQALGRPSREVVDVGSGGGLPGIPIAVIVPEVKV
                     LLVERRQKKYRALEHMISKLGLESRVRAKCETLEAMTKLPEDAEFWFRGVLPGPKLAL
                     LLTSAFPRADIKTIVLMKGPGWGDEKLGIMNEKGVKQIWKERFSDAQEVEYELPHGAG
                     SRTLVIV"
     gene            complement(2162..4066)
                     /gene="mnmG"
                     /locus_tag="PLH57_01030"
     CDS             complement(2162..4066)
                     /gene="mnmG"
                     /locus_tag="PLH57_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRK01222.1"
                     /translation="MEQNFDVIIIGAGHAGCEAALAAARLGCRTLLTTLNNDRVGYMS
                     CNPAIGGLAKGQLVKEIDALGGEMGINTDKSGIQFKKLNSSRGPAVRSSRAQCDKYVY
                     AANMKEVVESTPNLTLMQREIDSILVKDHRVIGVRTNWGEEIFSPTVVITTGTFLGGL
                     MHVGFQNQVGGRAGDAASVGLSNWLSKYGFRMQRLKTGTPPRLERRSIDFSQMSPQGG
                     DTPPLPFSFYYKPSVFPVLPQIDCWITYTNEQTHEIIGENFDKSPMFTGLIRGVGPRY
                     CPSIEDKVKRFSDKDRHQIFLEPEGLQTKEIYANGLSTSLPLEVQKKFLRTIPGLENV
                     EISRPGYAVEYDCLDPTQLKRTLETKDLPGLFCAGQINGTSGYEEAAAQGLIAGINAA
                     RSAKGLGEFVFDRTEGYIGVLIDDLIIKGAEEPYRMFTSRAEYRLILREDNADLRLGE
                     RARSIGLLSEESYRTLVEKKTAIEKELVSLGSSYFNPTNSVNEQLREVNTAEIKDRIS
                     AKQLLKRPEIDYSRLRKVGYLPATNQIDALEQVEIQVKYEGYIQRDLEMLESYRKNEE
                     MKIPVTLNYRDIAGLSAEVVEKLSLIRPETLGQALRISGITPAAVAAVHIYVKSLRST
                     WNKSENRASL"
     gene            complement(4206..6008)
                     /locus_tag="PLH57_01035"
     CDS             complement(4206..6008)
                     /locus_tag="PLH57_01035"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRK01223.1"
                     /translation="MKSRKKLKNAVKRRAVKRAPKAGRSLGDLGLFAPKAGELGDAAL
                     RVLKARYLLRNSAGEVIESPDQMFRRVAKAVASCESAATRASWEEKFFKIMRSLKFIP
                     NSPTLMNAGKPQGQLAACFVLPIEDSISSVFSSLHNAAIIHKSGGGTGFNFGQIRPRG
                     DLVQSSGGRASGPVAFLKIFDLATGVIKQGGTRRGANMAILPIDHPDIEQFIASKTAG
                     GITNFNISVGISDAFLQCLKRNGKWNLINPQTKEVVAQLPAKSLWSAICENAWRTGDP
                     GVVFLDTIEKQNPTPDIGKLDSTNPCGEQPLLPYESCNLGSISLAPYLSDKDLDWRTL
                     EQDIETAVRFLDNVIDVNWYPLPEIKNITEKNRKIGLGIMGWADALLHWKIAYDSEDA
                     LRRATLVAQRFHRVAVSASERLAKERGAFENFQNSLWAKRKMAKRRNATVTTIAPTGT
                     ISIIAGVSSGIEPIFSYAHERQALDGQRFYFLHSEVFNLVETGVLDRKSIERILRTGT
                     LSKVPKLSSTQKRVFRTAFEIAPIWHVRMQAVFQRMSESAVSKTINLPENATPADIGK
                     IYLKAHELGCKGITVYRDQSKTAQVLTRGIRKKF"
     gene            6038..>7182
                     /locus_tag="PLH57_01040"
     CDS             6038..>7182
                     /locus_tag="PLH57_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRK01224.1"
                     /translation="MMEPILSRNPATGATLKQIDQTPVAGIAALVETARYAQKSWAKV
                     PIRERAKCLLNAREYILNNLDDIADLIHLENGKPKFEAIVNDIIPVLDLLSQYAKKAP
                     KLMADKRIPMKLMLHRASYLQFWPKGVIAIISPWNYPFSIPFGEVAFALVAGNAVVFK
                     PSEVTPQIGLKIKEIFDNSGLPKDLLQIVLGDGARGAALVASKIDKVFFTGSVLTGKR
                     VMASAAENLTPVVLELGGKDPMIVLPDADLDYATSAALWGGFSNSGQACASVERILVH
                     ESIHDDFVSKLKEKLAALKHGQDLGVATYEKQKATYAEQLAELSSSNAQIVCGGHFSK
                     DRTYLEPTIVTNPKNHTDRPLERTKIYNDETFGPVVAVTTYKTIEEA"
BASE COUNT         1766 a         1815 c         1559 g         2042 t
ORIGIN      
        1 attcaaagtc ctgaatcaat tgatagtaag ccaatgctgt atcaaaacag ttcagttcct
       61 ctcgttgtat attctcgata agagctaatt ccagcgcctc tctatcggtt gttcttcgaa
      121 ttactattgg aacgtatttc agtcccgcaa ttttcgcggc gcgccatctt cgctcgccgg
      181 ctattaactg gtaaccccgt tgcgcttttc tcacaatgag gggttggatc aggccgtttt
      241 ccttgatcga ttgtgaaagt tcttcgattg ttatgtcgtc gaaaattttt cgtggttgaa
      301 attcgttggg cacaatttca tcgattgccg ccatactaat tccgggatgt ctgtctcgat
      361 tggaatcggg ctccgttgca ccacctccaa gagatgattc tgtcggagga gttggcggcg
      421 gcgtaactgc agctgtcggc aggagagagg ctaaaccctt gcctaatgcg ggacgcgtgg
      481 gtgctgatct aaatttttcc attttaatac tccattatta attggtgttc acttccgggc
      541 taccagaagt tgtggttcgc tgttgggaag acattgttcg agcaataagt tctttcgcta
      601 agctcaaata agcgacactc ccctttgagt cgatatcgta tagaagaatt ggctttccga
      661 aactcgagca ctcactcaaa cgaacgtttc gaggaatgat ggtctcaaaa acttttgaac
      721 cgaagtgggt acgaacctct cggaccacct cattggcgag attatttcga ccgtcataca
      781 ttgtaagtac gatgccttcg tcttggagcg ccggattcaa attctgacga atcaaaccaa
      841 ccgtattgag taactgagaa agtccctcga gcgcgaagta ctcgcattgc aatggcacta
      901 agaaactatc cgatgcggtt agtgcgttga cggttaaaag tcccaaagaa ggcggacaat
      961 ctatgaagat atagtcgtat tgattgcgaa tctccgctaa tgcggccttc agtttatttt
     1021 cgcgcgcaaa agacgaaacg agttcaatct cggctcctgt tagatcgttg ttgctaggag
     1081 caacaaacag gctgtcgatt tccgtcggct ggataatttg cgacatcgga actagaccga
     1141 tgattgcatg gtaaagattt ttttccgcga aggcctcttt acttacacct agagcagacg
     1201 acgcgtttcc ttggggatca agatcaagaa taagtgtttt tcgttcggcc accgccagtg
     1261 cggccgccaa attcaccgtc gtcgtggttt ttcctactcc acctttttga tttgtaaccg
     1321 ctattacgcg tcccataagt tcctctaatc cgctccagtc aacgctctat gaatattcag
     1381 tgctgttttt tccgatcaac caatgactaa gcagcgacct tacacagttt aggctaatag
     1441 agcctcgaac tttcgcaaca aaaaaatgtt ccccgtggaa caacttctta aactattacc
     1501 aaagttctac tgccggcccc atggggaagc tcgtattcga cttcctgtgc gtcggagaat
     1561 cgttccttcc agatttgttt cacgcccttt tcattcatga ttcccagttt ttcatcgccc
     1621 caacccggcc ccttcattaa tacaatggtt ttaatgtcgg ctcgcggaaa ggctgatgtc
     1681 aaaagcaacg ccaatttcgg ccccggaagc acaccgcgaa accaaaattc tgcatcctcc
     1741 ggcagcttgg tcatcgcctc gagcgtctca cactttgcgc gcacccgcga ttcgagccca
     1801 agtttagaga tcatatgttc aagcgcccga tattttttct gcctgcgctc cactagaagc
     1861 actttcactt caggaactat gaccgcaatc ggaattccgg gcagcccacc gccgcttccg
     1921 acgtctacca cctctcgaga aggcctcccc agggcctgaa tagccaacca ggtgtctgca
     1981 atgtgcttaa cagcgaactc ttttggggtt tttgcaccgg tcagattatt tgtttcattt
     2041 atggcgagca ccaattgcat atattttgct aatttgtcga ctgggggaac gctcgcgtag
     2101 gccaatgctt gcttacattc gtctaaactg ggccccgcgc cctctagaat aaccaactca
     2161 ctcataaaga agccctgttt tctgatttgt tccacgtgga acgtagagat ttaacataaa
     2221 tatgtactgc cgcgacggct gccggagtta ttccggaaat tctaagcgct tgacccaggg
     2281 tttctggtcg aatcaagcta agtttttcga caacctctgc cgaaagaccg gcaatgtcgc
     2341 gatagttcag cgttaccgga atcttcattt cttcgttttt acgataactc tctagcattt
     2401 ctagatctcg ctgaatgtag ccttcgtact ttacttggat ttctacttgc tcgagcgcat
     2461 ctatttgatt agtcgcgggc aaataaccga ccttgcgtag tcgtgaataa tcgatttcag
     2521 gccgttttaa gagttgtttg gcgctaattc ggtctttaat ttccgcggtg tttacttcgc
     2581 gaagctgctc attcaccgaa ttcgttgggt taaagtaact cgacccgaga gacaccagtt
     2641 ccttttcgat cgctgtcttt ttttctacca aagttcgata ggattcctca ctaagcaacc
     2701 ctatcgatcg cgcacgctcg cccaatcgca agtcggcatt gtcttcccgg agtataagtc
     2761 ggtattcggc gcgactggta aacattcggt acggctcttc ggcgcccttt ataatgagat
     2821 cgtcgatcaa aacgcctata taaccctcgg tccgatcaaa aacgaattcc ccgagacctt
     2881 ttgcgcttcg agccgcgtta atccctgcaa taagtccttg cgctgcagcc tcttcatacc
     2941 cggacgttcc attgatttga cccgcacaaa acagccctgg aagatctttt gtttcaaggg
     3001 tgcgcttcaa ctgggtgggg tcgagacaat catactcgac cgcatagcct ggccggctaa
     3061 tttcaacatt ttctaaaccc ggaatagttc gtaagaattt cttttggact tctaggggca
     3121 gacttgtgct caacccgttt gcgtaaattt ctttggtctg cagtccttct ggctctaaaa
     3181 aaatctggtg ccgatcttta tcggaaaacc gcttaacctt gtcttcaatt gaggggcaat
     3241 aacgcggtcc aacccccctt attagccccg taaacatggg acttttatca aaattttccc
     3301 caatgatttc atgggtttgc tcattggtat aggtaatcca acaatctatc tgcggtaaca
     3361 ccgggaaaac actgggctta taatagaaag aaaagggcaa tggcggggta tcgcccccct
     3421 ggggtgacat ttgactaaag tctatcgaac ggcgttcaag cctcggtggt gttccggttt
     3481 ttaggcgttg cattcgaaat ccgtacttac ttagccaatt ggacaaccca accgaagcgg
     3541 cgtcgccggc cctgccgccg acctggttct gaaatcccac atgcataagg ccgcccaaaa
     3601 aagttccggt ggtaattact acggtgggcg aaaaaatctc ttctccccaa ttggtgcgca
     3661 cgccaatgac tcgatggtcc ttaacaagaa tagaatcgat ctcgcgctgc attaacgtaa
     3721 gattcggagt ggactcaacg acctccttca tgttggccgc ataaacatat ttgtcgcact
     3781 gggcacgact tgaccgaact gccggccccc ttgagctatt taactttttg aattgtatac
     3841 cacttttgtc ggtattgatc cccatctcac cgccgagtgc gtcaatctct ttgaccagct
     3901 gtccctttgc gagtccgccg atagcgggat tacatgacat atacccaacg cgatcgttgt
     3961 ttaaagtcgt taacaacgta cgacaaccta gtcgcgccgc agcaagtgct gcttcacacc
     4021 ctgcatgtcc ggcgcctatg atgatgacat cgaaattttg ttccacgtgg aacggtttaa
     4081 tatcacgtca tcaaaatcta gtctatttag atattgttga ttattttgat atattactga
     4141 tattttccga cttatttgat ctgactattc caatccgttt gattcgaatt caagtaacgc
     4201 gaaacttaaa acttctttcg tattccacgg gttaggacct gagcggtttt actttgatcg
     4261 cggtaaacgg ttataccttt gcaaccgagc tcatgggctt ttagatagat tttcccaata
     4321 tcggcgggcg ttgcgttctc gggcaaatta atagtcttcg aaacagcgct ctcactcatt
     4381 ctttgaaaca ccgcttgcat ccgcacgtgc caaattggag ctatctcgaa cgccgttcta
     4441 aagactcttt tttgggtgga actaagcttg gggaccttgg agagagtccc cgttcgcaat
     4501 attctttcaa tactttttct atccaacaca ccggtttcga caaggttgaa tacttcggag
     4561 tgcaaaaagt agaatcgctg cccatccagc gcttgccttt catgggcgta cgaaaaaatc
     4621 ggttcaattc cactactaac tccagcaata atactaatgg ttccagtggg cgctatagta
     4681 gtgacagtcg catttcgtct ttttgccatt tttctttttg cccagagaga gttttgaaag
     4741 ttttcaaatg ctcctctttc ttttgccaat ctctccgaag cactcaccgc tactctgtga
     4801 aatctttggg ctaccagtgt ggctcttcgc agagcatctt cactgtcgta ggcaattttc
     4861 caatgaagaa gggcatcggc ccaccccata ataccgagac cgatttttcg atttttttct
     4921 gtgatatttt taatttccgg caacggatac caattaacat caatcacatt gtctaaaaat
     4981 ctgactgcgg tttcaatgtc ttgttctaaa gttcgccagt ctaagtcctt gtcagaaagg
     5041 tatggcgcta agctaatact gccaaggttg caactttcat aaggcagcag tggctgttcg
     5101 ccgcacggat tcgtactgtc tagctttcca atgtctggcg ttggattttg tttttctatg
     5161 gtgtccagaa acactacgcc cggatccccg gttctccatg cgttttcgca gattgcagac
     5221 cacaggctct ttgcgggcaa ctgcgcgact acttctttcg tttgcggatt gatcaggttc
     5281 cattttccat ttcgttttag acactgtaaa aacgcatcag aaattcccac cgaaatatta
     5341 aagttcgtaa ttcccccagc ggttttcgaa gcgatgaatt gctcaatatc cgggtgatca
     5401 attggcagaa tggccatgtt tgcgcctcgc cttgtgccac cttgtttaat cactccagtg
     5461 gctaggtcga agattttaag aaacgccacg ggtccgctag ctcgtccgcc ggaggattgc
     5521 accagatccc ctcttggtct tatttgccca aagttgaaac cggttccacc gccggacttg
     5581 tgaataatcg ctgcattgtg gagtgaacta aaaacgctcg aaatactgtc ttctatcggt
     5641 aagacgaaac acgctgcaag ctgaccttgt ggttttcccg cattcattaa ggtgggtgaa
     5701 ttggggataa atttaagact ccgcataatt ttaaaaaatt tttcttccca gctcgcccgt
     5761 gtcgcggcgg attcacaaga cgctactgct tttgcaactc tacgaaacat ctgatcggga
     5821 ctttcaatca cttctcccgc agaattgcgt aacaaatacc gagccttgag cacccgcaat
     5881 gcggcatctc cgagttctcc tgctttgggt gcaaaaaggc caaggtctcc cagtgagcgc
     5941 ccagctttag gtgctcgctt tacagccctt ctttttaccg cgttctttaa tttctttctg
     6001 cttttcacga cgcgtttcat gtcattataa tcattcaatg atggaaccaa ttttatcacg
     6061 gaatcccgcc accggggcga cactaaaaca gatcgaccaa acacccgtgg caggaattgc
     6121 cgcgctcgtc gaaaccgcac gttacgcaca aaaatcttgg gcgaaagttc cgattaggga
     6181 aagagctaag tgcctactca atgcgcgcga atatattcta aacaacctcg acgacattgc
     6241 tgatttaatt catttagaaa atggcaagcc caaattcgaa gcaatcgtaa acgatatcat
     6301 accggtttta gatcttctca gtcagtacgc caaaaaagct cccaagctca tggctgacaa
     6361 acgcattcct atgaagctca tgctacaccg agctagctac ttgcagtttt ggccgaaagg
     6421 cgtgattgct atcatttcac cctggaatta tcctttttcc attcctttcg gtgaggtcgc
     6481 ttttgccttg gttgctggaa acgcagtggt atttaaacca agcgaagtta ctccgcagat
     6541 cggcttaaag ataaaagaaa ttttcgataa ctctggtcta ccaaaagacc ttcttcagat
     6601 cgttctagga gatggtgccc ggggtgccgc acttgtagca agtaagatag acaaagtatt
     6661 ttttacagga agcgttctca ctggaaagcg cgttatggct tctgctgccg aaaacttaac
     6721 gcccgtagtg ctcgaattag gcggcaaaga tcccatgatt gtcttgcctg atgccgactt
     6781 agactacgca accagcgccg cactctgggg tggcttttct aattctggtc aggcgtgcgc
     6841 ctcagtagaa cgcattttgg ttcacgaaag tatccatgat gattttgttt ctaaattgaa
     6901 agagaaactt gccgcattga aacacggtca ggacttgggt gtcgcgacct acgaaaaaca
     6961 aaaagctacc tacgctgaac aactcgcgga gctttccagt tcgaatgctc agattgtttg
     7021 tggcgggcac ttttcaaaag atcgcactta tttggaaccg acaatcgtaa ccaatccaaa
     7081 aaatcacacc gaccggcctt tggaacgcac aaaaatctac aacgacgaaa cctttggacc
     7141 agtggttgcc gtgactacct ataagaccat agaagaggct at
//
LOCUS       DAOMXO010000025         7160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5457_length_7160_cov_4.387051, whole genome shotgun sequence.
ACCESSION   DAOMXO010000025 DAOMXO010000000
VERSION     DAOMXO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5457_length_7160_cov_4.387051"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..1037
                     /locus_tag="PLH57_01045"
     CDS             402..1037
                     /locus_tag="PLH57_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01225.1"
                     /translation="MFRNTHCIESLLEQFFHLGTRLREISVNAAIASGHVESQSKIFS
                     EIAKQIGSTSTLIAQLAERSKTITSQVSTRVLACIVDSGQREHFSFALRSIRESYNHT
                     TVRHVCLELSKKISDQLHEAQLALSHLKPEQDGLRQVNNRLSSIVVALRVLANTAAHG
                     EGTFFLSIAESIEETSDVATKICGSLQETVALINAALSERCACMRGELNAA"
     gene            1027..1764
                     /locus_tag="PLH57_01050"
     CDS             1027..1764
                     /locus_tag="PLH57_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004407786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK01226.1"
                     /translation="MLRKVLFDDGNHQWVFLGRDPSKPESVIDTNEYLIINGDKAMML
                     DPGGSEIFPTVLSEVSKVIDVRKIESYLCSHQDPDIMSSLPLWMALSPDARIHVSWLW
                     GKFIAHFGNSYVNRFSEIPDEGKTIRLGDQDLQLVPAHYCHSSGNFNLYDPKAKILFS
                     GDIGSALLPGGHPTFVEDFDSHIQFMESFHVRWLPSNEAKDRWVRRVRKLDIKMICPQ
                     HGAVFKDENVGKFLSWLENLEVGKTKE"
     gene            1772..2206
                     /locus_tag="PLH57_01055"
     CDS             1772..2206
                     /locus_tag="PLH57_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRK01227.1"
                     /translation="MNERKKQTRLRSELEKTSLSMKQVMKGLNEIDTLIRNIKDIAAK
                     TDLLALNASIEAARAGQAGKGFAIVADEVGRLSEKVQQAVSAVDACCINLRNSASTVM
                     TGLEDNQNLLDKEPRDSPSNSDSNPNTDSENEPPRLQGSKAA"
     gene            2210..3841
                     /locus_tag="PLH57_01060"
     CDS             2210..3841
                     /locus_tag="PLH57_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRK01228.1"
                     /translation="MAENDDLLPEFLAEAADIIEKIHGHLEKLPGAQDRSELITAIFR
                     NVHTLKGSSSMFNFSRTKSIAHELETNLGKFKESPQTLNDPEIQYIRDEVQKIDELLK
                     AKDQLDLVPGTLNSPGEPSADTRADNKELLPAASKTPQATVNATEEYIRVTVNRVNET
                     MNIVSEVFLIRNQMVYLVDRFKSGNLDQRDFFQTWDMLDGALRRGVGELERSTMSMRM
                     MSVQGLFNRMARSVQSYSAKTNKKILFQTVGENTELDKKILDVLGEPLIHLIRNAMDH
                     GIESPSDRIRSNKPEIGTIILSATISGNEAVVEVKDDGKGIDAKKILASAQKKGLDVS
                     HVTDDQSAIELIFLPGFSTAETVSEVSGRGVGMDAVKTSVTSLSGKVTIHTEVGKGST
                     FSIRLPVSMSLAPVVLIDIKGLKYAVPTSDILETKQVPFSWVKRNSGFDYVNFRDKYV
                     KCIDLRSIMDAEEKSHEENMRSCSIVFFRTSNETCAARVSSFERNTEIVVKPLSGLSP
                     NIPWVYGVAVLPTGEAIFVLSLAKILEEFEVTNAA"
     gene            3831..4310
                     /locus_tag="PLH57_01065"
     CDS             3831..4310
                     /locus_tag="PLH57_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRK01229.1"
                     /translation="MQPDEILKNQLNYRHTLSQDGKYASELSTYLQITIANSYFVIPI
                     SEVGEVSEFLPIRPYPSEVAGHVGVINLRGKVIPVISMEQFGGRKESKPNSRIVILNT
                     DTTASNSEQLALIVDSVKKISAPSAESGNVITLDNNPVRVLSGGLILESWGIKPCSR"
     gene            4298..5242
                     /locus_tag="PLH57_01070"
     CDS             4298..5242
                     /locus_tag="PLH57_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRK01230.1"
                     /translation="MLALKNEYGQSIGEMLQFVTFELLREGDKTLVMALNVQKIKEVV
                     ELCDLSPMPSGCENVLGIYDLRGCPVPVVDVLSLISVGKKSSHLGKDARLLICELQNI
                     WVGIPVNRTGRIFTCPSSSFLPPPVGSKIETRKIITGLIRKDATYVPVLDLDSLLDSV
                     GLIEEHTSRVTPQATYPGKRVLIADDSKVILKKLNQLFSQMGFIVDSVENGAEALKKI
                     ESEPHYDLVFTDIEMPILDGIAMAREIKGIPGYADTPILFNSALSNPALISDIEKEKL
                     GRYQVKFDSDLIVRHLEIIFGQSLEGSLTQPNSSSNST"
     gene            complement(5234..>7160)
                     /locus_tag="PLH57_01075"
     CDS             complement(5234..>7160)
                     /locus_tag="PLH57_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01231.1"
                     /translation="GSASPIESRGALVQYFKSKNFVGVIFGLWTALVTTGCPFLSNVT
                     APQAKIEQLRGRAILTLSDSVPVKRVQVFELNGSPEKRGALVWSAESLISEGQFLPGS
                     SDVPVDPNANLSGFFNIGLPASDCEPSIPGECHPKRFWDYTEINIQDVLLDQHRYQVE
                     IEFSVTDEPTKVTFTHRAREDVFQFNWPETLKTRDYGVISDRWAPWRFVETPSGNYVF
                     AALRNSEHPDRPLGYTALDSQHVRNVIPLKPLTPEFFGVYSEDPATRNITGPMSRPII
                     LENGRPVVAVMSNDHRSNYGMRLNVYKGKPPASVSSTTTAVLENEWTAIGSIETVYDG
                     WATISTPALVLGRIAGLADQHDTSPLIVSGQDPASTSLDPKNPQAFDLWYFAKNETWK
                     SLGTAGIIDSGSPIMAIDLAITKKGTLYGCVQYALNAIHVMKYSPNERRWNTLVSTHA
                     GFGRLDSEHSRVCQLAVEGDTLTAVGYLASPRYTLWVQKISEGGEVRTITPDLAAIIK
                     SKPSWYIQEVDQPVASQTARVHHTRLIQGDLYIALDDFGGLFPLSDYWPYSRSVHLIK
                     FNGTEWVRVRSSEFPLLPDMPPPSPGSAPQNDRTATIGAFITKHGDSLLYSGARWISP
                     ADPQIAEFQSIMTWELR"
BASE COUNT         2071 a         1659 c         1611 g         1819 t
ORIGIN      
        1 aactagttac accccgaggc agggctgatt gccgacaaaa cacagagatc tttaagccag
       61 tgaagaacga aagcgagtag caggggctta gcttaaggag ctaacgtaac cggaaacaag
      121 attttgtttc cggctgttgc ttccaagaag ccagaatgtt tccggaaaca aatcaccctg
      181 ccattaactg cttgattgca ttgacgtcgt agtatgaatt ttctataaaa aacgttcgat
      241 atagggatgc ttgagtgcgc catttcaacg caattttttg gtctgggcaa gcctttctga
      301 atctgaatta aaatcgttac ttttatctca gttcgcgcaa aataatttcc gataaaaaga
      361 ccgtgataga cgaacactac acgggcgaat gccttaagga cttgtttcga aatacccatt
      421 gcattgaaag tctgctggag cagttctttc atttgggtac gagacttaga gaaatttcgg
      481 taaatgcagc gattgcgtct ggccacgtag aaagccaatc taaaatattt agtgaaatcg
      541 caaaacaaat tggttcgact tccaccctca tcgcccagct tgcagagcgc tcaaaaacta
      601 tcacatctca agtcagtact agagtcctag cttgtattgt agattctggt caacgagaac
      661 acttttcgtt cgcacttcgc tccattcgtg agtcatacaa ccacaccact gtgcgccacg
      721 tatgtcttga attaagcaaa aaaatttctg accaactaca tgaggctcag ctagcgcttt
      781 ctcacttaaa accggaacag gacggcctgc gacaagtgaa taatcgactt tcgagtatag
      841 tagtcgcact tcgggtacta gcaaacaccg cagcgcatgg agaaggtacg tttttcttgt
      901 cgatcgcaga gtcgatagaa gaaacatcgg atgtcgctac gaaaatatgc gggtcgctac
      961 aagaaacagt ggccttgatc aatgctgcct tatcagaaag atgtgcctgc atgagaggag
     1021 aactcaatgc tgcgtaaagt actgttcgac gacggaaatc atcaatgggt atttcttgga
     1081 agggatccgt caaagccaga aagcgtgatc gacaccaacg agtatctcat aatcaatgga
     1141 gacaaagcga tgatgcttga tccaggaggc tcggaaatat ttccaaccgt acttagtgag
     1201 gtctcgaaag tcatagatgt ccgtaaaatt gagtcttacc tttgtagcca tcaggaccct
     1261 gacattatgt cttcccttcc gctctggatg gcattgagtc cggacgcgcg tatacatgtt
     1321 tcctggctct ggggaaaatt catcgctcac ttcggaaata gctatgtgaa tcgcttttcc
     1381 gaaattccag acgaaggcaa aaccattcga ttgggtgacc aggatcttca attggtacct
     1441 gcgcactact gtcactcgtc tggcaatttc aacctatatg acccgaaagc caaaattctt
     1501 ttcagcggcg atatcggttc ggcactctta ccgggtggac accctacctt cgttgaggac
     1561 ttcgattcac atattcaatt tatggagagc tttcacgtac ggtggcttcc gtccaacgaa
     1621 gcaaaagatc gatgggtccg ccgagtaaga aaactggata ttaaaatgat ctgcccccaa
     1681 catggggcag tatttaaaga tgaaaacgtc ggaaaatttt tatcgtggct cgaaaatcta
     1741 gaagttggaa agactaagga atgagacata tatgaacgaa cgaaaaaaac aaactagact
     1801 ccgatcggaa ttagaaaaaa ccagcctttc tatgaagcag gtaatgaagg gcttaaatga
     1861 aatcgacact ctaattcgca acattaagga cattgccgcg aaaactgact tactggcact
     1921 aaacgcctcg attgaagctg cacgggccgg gcaagcgggc aaaggttttg ctattgtcgc
     1981 cgacgaggtt gggcgtttat cagaaaaagt acagcaggcg gtcagtgccg tagatgcatg
     2041 ttgcataaat ttacgtaaca gcgcgagcac agtaatgacg ggattagaag acaatcaaaa
     2101 ccttctggac aaggaacctc gagattcacc atcgaactcc gattcaaacc caaacacaga
     2161 ttcagaaaac gaacccccta ggttgcaggg ttcaaaggca gcttaaacaa tggcagaaaa
     2221 cgacgatcta ttaccggaat ttttagcgga agcagccgat attatagaaa aaattcacgg
     2281 ccatctggaa aaacttccag gggcccaaga tcgttcggaa ttaattaccg cgatatttcg
     2341 caatgtacat accctcaagg gtagtagttc gatgtttaac ttttctcgca ccaaatcaat
     2401 cgcgcacgaa ctcgaaacta atttaggaaa atttaaagaa tccccgcaga ctttgaatga
     2461 tcctgaaatt caatatattc gagacgaagt tcagaaaatc gacgaactac taaaagcaaa
     2521 agaccagctt gatcttgttc ccggaacatt aaactctccc ggagaaccct ctgccgatac
     2581 cagggccgac aataaagaac tacttcccgc tgcttcgaaa accccacaag ctacagtgaa
     2641 tgcgacggaa gagtatattc gagttaccgt aaatcgcgtc aatgaaacta tgaatattgt
     2701 ttccgaggta tttctcatcc gaaaccaaat ggtttacctg gtggatcgat ttaagtctgg
     2761 caacctagat caacgcgatt tctttcaaac atgggacatg ctcgacggag cgctgcgcag
     2821 aggggtcggc gaactcgaac gcagcacgat gagcatgcgc atgatgtcgg tccaaggtct
     2881 atttaatcgc atggcccgta gcgtacaaag ctacagcgcg aaaactaata aaaagattct
     2941 ctttcaaaca gttggagaga atactgaatt agataaaaaa atcttagatg tattaggcga
     3001 gcccttaatt cacctgattc gaaatgctat ggatcacggc atcgaatcac cctcggatcg
     3061 aatcagaagc aataaaccag aaattggaac tattattttg tctgccacaa tttcaggtaa
     3121 cgaagcagta gtggaagtca aagatgacgg taaaggcatc gatgcaaaaa aaattctggc
     3181 ctccgctcaa aaaaagggac tcgatgtctc gcacgtaaca gacgatcagt cggccattga
     3241 actaatattt ctaccgggtt tttcgaccgc tgaaaccgtt agtgaggttt ctggtcgtgg
     3301 cgttggaatg gacgctgtaa aaacctctgt cacatcgctc agcggcaaag taactatcca
     3361 taccgaagtt ggaaaaggct ccacattttc gattcgcttg cctgtaagca tgtcgctagc
     3421 accagtggta ttaatcgaca taaagggatt gaaatacgcg gtccccacta gcgatattct
     3481 tgaaaccaag caagtaccgt tttcgtgggt aaagcgaaat agcgggttcg attatgttaa
     3541 tttccgcgac aagtacgtaa aatgtatcga cctacgctcc atcatggatg cggaagaaaa
     3601 aagtcacgaa gaaaatatgc gttcctgctc gatcgtattt tttcgtactt ccaatgaaac
     3661 ctgcgccgct cgcgtttcta gtttcgaacg caacaccgaa atcgtcgtaa aacccctttc
     3721 tggactgtcc cctaatattc cgtgggttta tggcgtcgca gtactaccaa cgggagaagc
     3781 aattttcgta ctgtcgctgg cgaaaattct agaagaattt gaggtcacca atgcagcctg
     3841 atgaaatttt gaaaaatcaa cttaactatc gtcatacact ttctcaggat ggcaaatacg
     3901 cttccgagct cagtacctat ttgcaaatta cgatcgcgaa ctcctatttt gtaattccaa
     3961 tttctgaggt gggtgaagtt tctgaatttt tgccaattcg gccgtaccct tccgaggtag
     4021 cgggacatgt cggggtgatt aatttacgag gaaaagtcat acccgtaata tccatggagc
     4081 agttcggtgg ccgcaaagaa tcgaaaccaa atagccgcat tgtgattctt aataccgata
     4141 caacagcttc caattccgag cagttggctc ttattgtcga ttctgtaaaa aaaatatcgg
     4201 cacccagcgc cgaatctggc aacgtaatca cgctcgataa taatccagtg cgcgttcttt
     4261 ctggaggatt aattcttgaa agctggggga tcaaaccatg ctcgcgttaa aaaatgaata
     4321 tggccaatcg atcggcgaga tgttgcagtt tgtgactttc gagcttctcc gagagggcga
     4381 taagacgctg gtaatggcgc ttaatgttca aaaaattaaa gaagtcgtcg aattatgcga
     4441 tctgtcgcca atgccgagtg gatgtgaaaa tgtacttgga atctatgatt taagagggtg
     4501 cccggtacca gtggtagacg ttctctcgct tatttcagtt ggaaaaaaaa gttcccatct
     4561 tggcaaagac gcccgtcttc tcatctgtga attacagaat atttgggttg gaattccggt
     4621 caatcgcacg gggcgcatat ttacctgtcc cagctctagc tttttaccac ctccagtggg
     4681 atccaaaata gaaactagaa aaataattac cggattgatt cgtaaggatg ctacctatgt
     4741 gccagtcctc gatttagata gcttacttga ttcagtcgga cttatcgaag aacacacttc
     4801 tcgagttact ccacaagcca cttaccccgg aaaacgagtg ctcattgctg acgactcaaa
     4861 agtaatctta aaaaaattaa atcagttatt ttctcaaatg ggctttatcg tcgattcagt
     4921 agaaaacgga gcggaggctc tgaaaaaaat tgaatctgaa cctcactacg acctcgtttt
     4981 taccgatatc gaaatgccga tcctggacgg tatcgcaatg gcccgtgaga ttaaaggtat
     5041 tccaggttat gcggatacac cgatattatt taattccgca ctttcgaacc ccgcactcat
     5101 ttcagacatc gaaaaggaaa aattaggacg ctaccaagtt aaattcgatt cggatcttat
     5161 agtgcgacac cttgaaatta ttttcgggca atcattggaa ggttcgctaa cccagccgaa
     5221 ttcgtcctcg aactcaacgt agttcccatg tcatgatcga ttgaaattct gctatttgcg
     5281 ggtcggccgg cgaaatccag cgggctccac tgtataacaa agagtcccca tgtttcgtaa
     5341 taaaggcccc aatcgtcgcg gtacgatcat tctggggagc cgaacccgga ctaggcggtg
     5401 gcatatctgg cagtagtgga aattcactgg accgcacacg cacccactct gttccgttaa
     5461 attttataag gtgaacgctt cgactgtaag gccagtaatc ggataacgga aacaatcccc
     5521 cgaagtcatc caatgcgatg tagagatcgc cctgaatcaa tcgagtgtga tgaacccgtg
     5581 ccgtttgact ggcaacaggc tgatctactt cctgaatata ccagctaggt tttgatttaa
     5641 ttatcgctgc caagtctggc gtgatcgttc gcacttcacc gccttcagaa atcttttgta
     5701 cccaaagcgt ataacgcgga gacgccaaat agccgaccgc tgttagagtg tcaccttcta
     5761 ccgcgagctg acatacacgg gagtgttcac tgtcgagtcg cccaaaaccg gcatgagtag
     5821 aaaccaaggt attccatctt cgctcgttcg gactatattt cattacatga attgcattca
     5881 gggcatactg cacgcagccg tataatgtac ctttttttgt tatcgccaga tcaatcgcca
     5941 ttataggcga accggaatca atgataccgg ccgttccaag ggacttccat gtttcgttct
     6001 tcgcgaagta ccaaagatca aaggcctggg gatttttcgg atcaagacta gtggaagcag
     6061 ggtcctgccc cgacacaata agcggagacg tatcgtgttg atcagcaagt ccagcgatcc
     6121 gccccaaaac cagtgctggg gtagaaatgg ttgcccatcc gtcgtacacc gtttcaattg
     6181 agccgatcgc agtccattcg ttttccaaaa cggctgttgt ggtggacgag acgctagccg
     6241 gaggctttcc tttataaaca ttcaaccgca tgccgtagtt gctgcgatga tcgttcgaca
     6301 taacggccac taccggtcgg ccattttcca aaattattgg tcgggacatt gggcccgtaa
     6361 tatttcgcgt tgctggatct tcggaatata caccaaaaaa ttccggcgtt agaggtttga
     6421 ggggaatcac attccgtacg tgctgcgagt cgagcgccgt gtaaccaagt gggcgatccg
     6481 ggtgttcgga atttctcaaa gctgcaaata cgtaattgcc cgacggagtt tcgacaaaac
     6541 gccacggagc ccagcgatcg ctaattactc cgtagtcgcg ggtttttaat gtctctggcc
     6601 agttgaattg aaacacatct tcgcgggcgc ggtgagtaaa agttaccttt gtaggttcat
     6661 cagtaacgga aaattcgatc tccacttgat aacgatgttg gtccagcaga acgtcttgaa
     6721 tatttatttc agtgtagtcc caaaaccttt ttgggtgaca ttctcccgga atcgatggct
     6781 cacaatctga agccggtaat ccaatattaa agaaaccact taaatttgca ttcgggtcta
     6841 ctggtacgtc gcttgaacca ggaagaaact gaccttcgct tattagactt tcggcgctcc
     6901 aaacaagtgc tccacgtttt tcgggcgaac cattcaactc aaacacttgt acccgtttta
     6961 ccggcaccga atccgaaagc gtcaaaattg cccgcccacg cagttgctca atttttgctt
     7021 ggggtgcggt tacgttgcta aggaaggggc accccgtagt aaccagggcg gtccatagcc
     7081 caaatataac ccccacaaaa tttttcgatt taaagtattg caccaaagcc cccctcgatt
     7141 cgattgggga agcactacca
//
LOCUS       DAOMXO010000026         7148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5473_length_7148_cov_4.922459, whole genome shotgun sequence.
ACCESSION   DAOMXO010000026 DAOMXO010000000
VERSION     DAOMXO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5473_length_7148_cov_4.922459"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLH57_01080"
     CDS             complement(<1..511)
                     /locus_tag="PLH57_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01232.1"
                     /translation="MTAKVSLRFANLIFTLLVFCSLGQATAADHCKRVVRGGFAAEQR
                     ALGIGADSSSVLENLRDAQALAAAGDTVGAARAINKGLQSPSSATIVTPAILEGVKAA
                     ELVYLAAPASKTEKRSYRKILQCMAKSPARGQTLHTVVGGTGAIALLLASDWTTDLED
                     IGLLGVAGVA"
     gene            complement(529..861)
                     /gene="grxD"
                     /locus_tag="PLH57_01085"
     CDS             complement(529..861)
                     /gene="grxD"
                     /locus_tag="PLH57_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HRK01233.1"
                     /translation="MNTNPLVTKVEEITKTNPVVVFMKGTAQFPQCGFSARACQVLKA
                     SGVNDFTTIDVLSDDPLWDALEAFTQWPTVPQIFIGGEFIGGCDIISEMYERGDLATL
                     LKDKKVTA"
     gene            complement(858..1115)
                     /locus_tag="PLH57_01090"
     CDS             complement(858..1115)
                     /locus_tag="PLH57_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015162692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRK01234.1"
                     /translation="MNATQLKNRIETLAAGTQVVELVDLTGTQDHWQALIVSPAFEGK
                     TMIQQHRMVFELLKDEVASNEVHALTLRTFTPAQFEKGPRK"
     gene            1169..1900
                     /locus_tag="PLH57_01095"
     CDS             1169..1900
                     /locus_tag="PLH57_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK01235.1"
                     /translation="MKKMNAVHLKDLSFSFDSKKMGPPLLTVDSYSAALGTLNLLLGA
                     NGVGKSTLLRLIAGKHLVPNGGIQVLGRDPFSQPMGMADVCLVSGYFPLTMDLEVAEL
                     LAAAEKKPEYAGSGLKKELVDLLEVDPGWRMHRVSTGQRRRVDLLLALLADPKVLLLD
                     EVTSDLDILCRIDLLEWLADRAAKDRKTIFLATHILDGLEKWADQLLFLGFGKVLYQG
                     AIPKRDSLVRFAVKTMRKDIKAKKK"
     gene            complement(2011..3417)
                     /locus_tag="PLH57_01100"
     CDS             complement(2011..3417)
                     /locus_tag="PLH57_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRK01236.1"
                     /translation="MKTQKILKKVALTVTGIASISSAALADVNVQLFNPAMNRSFTLT
                     EDSAADTKAKSMFLGATYNYVNRPIVELTDDRKERLGDLLTSMSTLNITGGYHFNDVF
                     FLGLDIPVNMVKVPGEAQEFALGDSRLMGKLRLNDRKATVTFALVPELYIPTGNADLY
                     LSDGTVGAGAKVVAERAWGKLKVAANLGYRYTSDGRFRDINYGNRLSAGLGANLSLTR
                     LWALNAEGKGTLVLPRNGKQNPGEFYTGVRYQPRNEIALAGGLAIGAINGVGSVDYRG
                     IIGVQFLATPPEAPAPVKPVIVSAVQKPQPRVFWEPKQIRVTEEIKFHHNSAVLTDSG
                     KRLLDEVAAVIKQNVDHFKSITVEGHTNRLGSHPYNMKLSQERAASVREYLASRGVDM
                     NRLKVIGYGKTRPKMIAGLSKEAQLAADRRVEFKVEALSPMEVSAVKKRKEAELNKEL
                     NRASISVPAGSAAAPKAE"
     gene            complement(3444..4703)
                     /locus_tag="PLH57_01105"
     CDS             complement(3444..4703)
                     /locus_tag="PLH57_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01237.1"
                     /translation="MSMNAVRLLALFSLVVAVAAAGCGGNASVMRGKPGGGDGSSGGA
                     VQPSTEAGTDADESSDDDLIVEAPVGASGPQDGSPIDGTDGDDGDDANANNGKDGDDA
                     LANNGNGEDGANGTGKDGKDGIDGKDGKDGETRIVEVPAVQEPTIITVRGEKGDPGLT
                     ACNRGEVFPMPAMSAIPMSPSEMASFVTPLLETPSSEDIIKKIKKRNKKRSDDVPMSI
                     SKLSLGENSPGKKLVHDSQVLFKIPVTLPPRKYIVGASKVFINIRTIKTRGDGKKGDG
                     HDETEFFALLGHNPKDMIMSGYRYDAVSQWYWATAFNNPAFPETQNRAYSQMMVDSIA
                     RQTAGKRSSQPIEVTVAVDMNQIIAGSAYNDILDYLYRDVTDYTRAANVDVNIAVGDD
                     TKVLDAKIEMTFLKDTCAEAEDRGIQK"
     gene            4870..5202
                     /gene="cyaY"
                     /locus_tag="PLH57_01110"
     CDS             4870..5202
                     /gene="cyaY"
                     /locus_tag="PLH57_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HRK01238.1"
                     /translation="MLDEKNYRVLVKQAFDRVEQAFNDVDPDVVEVETSQGTLRVQVA
                     NAGACILSPQPSVRQIWLAVAAKGIAFHFNYNEATKEWDDDKGQGITLFQFLQNYVKE
                     AARVEISL"
     gene            complement(5138..5716)
                     /locus_tag="PLH57_01115"
     CDS             complement(5138..5716)
                     /locus_tag="PLH57_01115"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HRK01239.1"
                     /translation="MTKIENTLEILILQHPQEKSKDLATAPVLVQSLSRCQIRVGLSW
                     ANLAKAVGRPVDPKKWGVLFVGSQASSQKIKSIPQGLPLEGVIALDGNWSQAKALWWR
                     NAWLLKLHRIVLNPKKPSLYNKLRREPRKNCISTLESVVSVLEMFGSESDKKAASLLQ
                     QKFEEFVAEKLLKAKSPHEPLPSRSSEETEKA"
     gene            complement(5740..6669)
                     /locus_tag="PLH57_01120"
     CDS             complement(5740..6669)
                     /locus_tag="PLH57_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK01240.1"
                     /translation="MTLPLGPLAAFMASVTWAIGVSTYTRLSAKYPAYQVHLARIMIA
                     LPLFTIAVVINGGFSEFLKITDHNLVWGFVSALASFALGDVLFLWSTVALGAPAALAI
                     ASTYPFWSALAGVFLKGEALTGMKAFGLLLVVGGTVTVILSGYRRSNIDVKRELEKRI
                     TPAREKKLLEQYSVGLTFAFLTSLAWALNTYSGMQTAHGVSAPVANFIRMGMSLFLCP
                     LVGSLLTRARPRALPFKEFKRYGWVFAFEAFGGSMCYIYGLMHAPLAVASALSSLAPV
                     LAVPIGLLFGLEKFSFQKTLGIAMVVAGISLLV"
     gene            complement(6689..>7148)
                     /locus_tag="PLH57_01125"
     CDS             complement(6689..>7148)
                     /locus_tag="PLH57_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01241.1"
                     /translation="LRNKLGINGNLHINRSKTVPVGSLAVNGNGHNIALESRADLLSL
                     RGSRVPIVAVLDGEPGLLDNLGSVIGVPQETLAVRKHSKSGRPVDVYKYHHLDPESIA
                     EAAIGLLERAASEEIIVSSQVIAVANETETTSASSYGTVSYDDGDSETRH"
BASE COUNT         1749 a         1745 c         1791 g         1863 t
ORIGIN      
        1 ccgccacacc cgctactcca agcaaaccta tatcttcgag gtctgtagtc cagtcacttg
       61 ctaaaagtag cgcgatcgca ccagtaccac ccaccactgt gtgaagtgtt tgaccccgag
      121 caggtgactt cgccatgcat tgtagaattt tgcgatagct tcgcttttct gtttttgaag
      181 caggtgcggc caaataaaca agttcagcag cctttacacc ttcaagtatt gcaggcgtta
      241 ctatggtcgc cgacgacggg gattgcagac ctttgttgat cgctctggca gcaccgacgg
      301 tgtccccggc ggctgccaat gcttgtgcgt cgcgaagatt ttctaagaca ctgctggaat
      361 ctgccccgat tcctagggcc cgctgttccg cagcaaaacc cccacggacc actcttttgc
      421 aatggtcggc cgcagtagct tggcccaacg aacaaaagac caaaagcgtg aaaattaaat
      481 tcgcgaagcg aagcgaaact ttagctgtca tttaatacct aaaactcgtt aagccgtaac
      541 ttttttgtct ttcagtagcg tggccaaatc cccgcgctca tacatttctg aaatgatgtc
      601 acagccacca atgaattcac cgccaataaa aatctgaggc actgttggcc attgggtaaa
      661 tgcttcgaga gcgtcccata gcgggtcgtc cgataaaaca tcgatcgtcg taaagtcgtt
      721 taccccagaa gctttaagca cttggcaagc gcgcgccgaa aagccgcatt gtgggaattg
      781 tgcggtacct ttcataaata cgactacagg gttggttttg gtgatttctt ctactttggt
      841 gaccaatggg tttgtgttca tttgcgtggt cctttctcaa attgcgcagg tgtaaacgtg
      901 cgaagagtta gtgcgtgaac ttcgttcgag gctacttcat cttttaaaag ttcgaagacc
      961 atgcggtgtt gctgaatcat ggtcttgcct tcaaacgctg gtgaaacaat gagcgcctgc
     1021 cagtggtcct gggtaccggt aaggtcgacc aactccacta cttgggtgcc ggcggctagt
     1081 gtttcgattc tattttttag ctgtgttgca ttcatgtttt aattctctcg agttgagctt
     1141 cggataccac attgctagtc tggtttcgat gaagaaaatg aacgcggtgc atttaaagga
     1201 cctgagtttt tcctttgatt ccaagaagat ggggccgccc ttactcacgg tcgactctta
     1261 tagtgccgca ttgggcacgc tgaacctttt gcttggagct aacggcgtgg gcaaatcaac
     1321 gcttttgagg ctcattgccg gaaagcacct cgttccaaat gggggaattc aggtactggg
     1381 ccgagaccct ttttcgcagc ccatgggcat ggccgacgtt tgtctcgtgt cggggtattt
     1441 tcctcttacg atggatcttg aagtcgcgga gctcctcgcg gcggctgaga aaaagcccga
     1501 gtacgcaggc tcgggactaa aaaaagagct agtagattta ctcgaagtcg acccaggctg
     1561 gagaatgcac cgagtttcga cggggcaacg ccgccgcgtc gatctgctac tggccttgct
     1621 ggcagatccc aaagtgctat tgctcgacga agttacatca gatctcgata ttttgtgcag
     1681 aatcgatctt ttggaatggc ttgcagaccg agcagccaag gatcgaaaaa ctatttttct
     1741 tgcgactcac attctcgacg gcctcgaaaa atgggcagat cagctgttat tcttggggtt
     1801 cggaaaggtg ttataccaag gggcgattcc aaagcgagat tcccttgtgc gtttcgccgt
     1861 aaaaacaatg cgcaaagata tcaaggccaa gaaaaaataa aaaaaaactc ccgcaaaacc
     1921 atttgcggga gtttttcgtt tcagagtgcg acgctagttt ctgaacccag caggttcaac
     1981 aaccaacacc tcgctcaaaa aatgagccct ctactcagcc tttggagcag cggcggatcc
     2041 tgcaggaacc gatatgcttg ctctattcaa ttccttatta agttcggctt ctttgcgttt
     2101 tttcacagca gaaacttcca ttggagaaag tgcttcaact ttgaattcta cgcgacggtc
     2161 agcggcgagc tgagcttctt tcgaaagacc agcgatcatc ttcggccgtg ttttgccgta
     2221 gccgataacc ttcaagcggt tcatgtcgac accgcgagaa gcaaggtatt cacgtacgct
     2281 agcagctcgc tcctgggaga gtttcatgtt gtacgggtgc gaacccaagc ggttggtgtg
     2341 cccttctacc gtgattgact taaagtgatc tacgttttgc ttgatcactg cagccacttc
     2401 atcgagcagg cgtttgccag aatcggtgag tactgcgctg ttgtggtgga acttaatttc
     2461 ttcagtcacg cgaatctgtt taggttccca gaaaacgcga ggctgtggtt tttgtaccgc
     2521 cgatactatt actggtttta ctggagctgg agcttctggc ggtgttgcca aaaactgaac
     2581 accaataata ccgcgatagt ctacgctacc aacaccgttg atcgcaccga tggcgagacc
     2641 gccggccaac gcgatttcat tgcgtggttg gtaacgaacg cctgtgtaaa attcgccggg
     2701 gttttgcttg ccgttgcgag gcaataccag agtacctttg ccttcagcgt ttaacgccca
     2761 aagacgagtg agcgaaaggt tcgctcccaa gccggccgac agacggtttc cgtaattgat
     2821 gtcgcgaaaa cggccatccg acgtgtagcg atatccgagg tttgcagcca ctttgagttt
     2881 gccccatgcg cgttctgcta ctactttagc gccagcaccg acagttccgt ccgacaaata
     2941 aagatctgca ttaccagtag gaatatagag ttcgggaacc aaagcgaaag ttacggttgc
     3001 tttgcggtcg tttaatcgaa gtttacccat caagcggctg tcgcccaaag cgaattcttg
     3061 agcttcgcca ggtaccttca ccatgttcac gggaatgtcc aatccgagaa agaacacgtc
     3121 gttgaagtga taaccacccg taatattgag agtgctcatc gaagtaagta gatcaccaag
     3181 acgttctttt cgatcgtccg tgagttccac gatcgggcga tttacgtagt tgtaagtcgc
     3241 gccaaggaac atcgattttg ctttagtgtc cgccgccgag tcttcagtca acgtaaagct
     3301 tcggttcatt gcggggttga acaattgaac gttcacgtcc gcaagggccg cgctagagat
     3361 cgacgcgatt ccagttactg tcagggccac tttctttagg attttttgag ttttcatatt
     3421 tttttcacct tatttactac tacttatttt tgaattccgc ggtcttcagc ttctgcacag
     3481 gtgtctttca agaaagtcat ttcaatcttg gcatcaagta ctttagtgtc gtcgccaacg
     3541 gcgatgttca cgtcaacatt tgctgcacga gtgtaatcag ttacatcacg atacaaataa
     3601 tcaagaatgt cattgtaagc actgccagcg atgatttgat tcatgtctac tgccaccgtg
     3661 acttcgatag gttggctcga gcgcttgcct gcggtctggc gagcaatgct gtcgaccatc
     3721 atttgcgaat aagcacgatt ctgggtttca gggaaagctg ggttgttgaa cgcggtcgcc
     3781 cagtaccact gtgaaaccgc gtcgtagcgg taacccgaca tgatcatgtc tttagggtta
     3841 tggccgagaa gagcgaaaaa ttctgtttcg tcgtggccgt cacctttttt cccgtcgccc
     3901 cgagttttaa tagtgcgaat gttgatgaac actttacttg caccaacgat gtattttcgt
     3961 ggaggcaaag taactgggat tttgaacaat acttgggagt catgtaccaa ctttttgcca
     4021 ggcgagtttt cgccgagaga tagtttggaa atactcattg gaacatcatc ggagcgcttc
     4081 ttgttacgct tcttgatttt tttaatgatg tcttccgacg aaggagtttc aagaagaggt
     4141 gttacgaacg aagccatttc ggagggcgac attggtattg ccgacatcgc tggcatcggg
     4201 aacacttcac cgcggttaca agcagttaat cctggatcgc ctttttcacc acgtacggtg
     4261 atgatcgttg gttcctgaac tgcgggaact tcgacaattc gagtttcgcc gtctttaccg
     4321 tctttaccgt cgataccgtc tttgccgtct tttccagttc cgttagcgcc gtcttcgccg
     4381 ttaccgttat tggccaatgc gtcgtcgccg tctttaccgt tgttggcatt tgcgtcgtcg
     4441 ccgtcgtcgc catcagttcc gtctattgga gagccgtctt gaggaccgct agcaccaacc
     4501 ggagcttcaa ctatcaaatc gtcgtcggag ctttcgtctg cgtcggtccc agcttcagtg
     4561 cttggctgca cagcgccgcc ggagcttcca tcgccgccac ctggctttcc gcgcatcact
     4621 gaggcattgc cgccgcagcc agctgccgcc actgcgacca ctaaactgaa tagggctagt
     4681 aaacgaactg cattcatcga catgtcgatt ctccttaaaa gtatgaacag aaatttgagg
     4741 cacactatag agaaaacaac gcaatgagtc aataagattt atcaaaacat tcaggtatta
     4801 ttagttcaat aatattaatg ggttatattt tattgacgag ttaccccgag ctgtggtcct
     4861 ttgtttttca tgctagatga gaaaaattat cgggttttgg tgaaacaagc gttcgatcgc
     4921 gtagaacaag cctttaatga tgtggatccc gatgtggtgg aagtagagac ctcgcagggc
     4981 acactgcggg ttcaggtggc caacgcaggc gcctgtattt tgagtcccca gccttcggta
     5041 cggcaaatct ggttagccgt cgcagccaag ggcatagctt ttcattttaa ctataatgaa
     5101 gcgaccaaag agtgggacga tgacaaagga caaggtatta cgctttttca gtttcttcag
     5161 aactacgtga aggaagcggc tcgtgtggag atttcgcttt gagcaatttt tcggcgacaa
     5221 attcctcgaa tttttgttga agcaacgaag cggctttttt gtcggactct gatccaaaca
     5281 tttctaaaac cgaaaccaca gattcaagag ttgaaataca gtttttgcgc ggttcgcggc
     5341 gaagtttgtt gtagagcgaa ggcttcttgg gattcaaaac aatccgatgc agttttagca
     5401 gccaagcatt gcgccaccaa agggctttag cttgtgacca attgccgtcg agtgcgatta
     5461 cgccttcgag tggcaacccc tgcgggatag atttgatttt ttgagacgaa gcttgagagc
     5521 cgacgaagag tacgccccat tttttggggt ccaccgggcg ccccactgcc ttggcgagat
     5581 ttgcccaaga aagacctact cgaatttggc atcgagaaag cgattgaact aagaccggcg
     5641 cagtggccaa gtccttagat ttttcttgag gatgttgcag aatcaggatt tcgagcgtgt
     5701 tttcaatttt agtcatacgt taaatcggcc gcgccacggt tacaccaaaa gagaaatccc
     5761 tgcaacgacc atcgcgattc ccaaagtttt ttgaaaagaa aatttttcga gtccaaacaa
     5821 aaggccgatg gggactgcaa gtaccggagc gaggctagag agcgccgatg caacggccag
     5881 tggcgcgtgc atgagaccgt agatgtagca cattgagcct ccgaaagctt cgaacgcgaa
     5941 gacccaaccg tatcgtttga attccttgaa aggtaaagcg cgaggtcgcg ccctagtgag
     6001 gagtgaacct acgagtgggc acagaaacag cgacataccc atgcgtatga aattggcgac
     6061 tggtgccgaa actccgtgag cagtttgcat gccggagtag gtatttaagg cccaagcgag
     6121 ggacgttaaa aaagcgaacg tgagcccaac tgagtactgt tcgagtaatt ttttttcccg
     6181 cgccggagtg atgcgttttt caagctctcg tttgacgtca atgtttgatc gacggtatcc
     6241 cgacagaata acggtgacgg tacctccgac caccagtaac agcccgaaag ctttcatgcc
     6301 ggtaagagcc tcgcctttta aaaacacgcc tgcaagcgct gaccaaaaag gataagtcga
     6361 ggcgattgca agtgcagcag gagcgccgag ggccacagtc gaccaaagaa aaagtacgtc
     6421 gccgagtgca aaactcgcca acgcactgac gaagccccac accaaattgt ggtcggtaat
     6481 tttgagaaat tcactaaaac caccgtttat aacgaccgca atcgtgaaca acggcagagc
     6541 tatcatgatg cgtgcgagat gtacttggta ggcagggtac tttgcactta ggcgagtata
     6601 agtagaaacg ccgatcgccc aagtcaccga cgccatgaac gccgcaagag gaccaagagg
     6661 cagagtcatt gaaaggtctt tctagctatt aatgtctagt ttcagaatcc ccgtcgtcgt
     6721 acgaaacagt tccgtaagac gaagcagagg tggtctcagt ttcgttcgcc actgcgatga
     6781 cttgggaaga aacaataatt tcttcgctcg ccgcgcgttc aagcaatccg atggccgcct
     6841 cagcaatcga ttctggatcc aagtgatggt atttgtaaac atcgactggt cgccccgact
     6901 tgctgtgttt tcggacggcc agagtttctt gcggaacgcc aatgacgctt cccaagttgt
     6961 cgagcagccc aggttcacca tcgagcactg ccactatagg gactcgagac ccacgtagag
     7021 agagtaaatc agcccgcgac tctaatgcaa tgttgtgtcc gttaccatta actgccaggc
     7081 ttcctacggg taccgttttt gagcggttga tgtgcagatt gccattaatt ccaagtttgt
     7141 ttcttaaa
//
LOCUS       DAOMXO010000027         6895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5763_length_6895_cov_4.842251, whole genome shotgun sequence.
ACCESSION   DAOMXO010000027 DAOMXO010000000
VERSION     DAOMXO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5763_length_6895_cov_4.842251"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLH57_01130"
     CDS             <1..217
                     /locus_tag="PLH57_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK01242.1"
                     /translation="EKDFEEVFEVSFTTKSAEEGTGLGLAIARRFIRAYGGELSVEMS
                     VPNERTVFLIEMPTISEESHQEVTTDV"
     gene            210..1418
                     /locus_tag="PLH57_01135"
     CDS             210..1418
                     /locus_tag="PLH57_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK01243.1"
                     /translation="MSEVRKPNNLLLVVDDEKDIVQAYIDFLVIEGKSERKSSRSKGN
                     ANENLEQNTETYEILKAYNGPEALKVVEGELKNGRRIAGGFFDVKMDGGMDGLQTIKE
                     IWKRDPKVNCTIVTAYQDRSVQDINQLFGESFRDQWDYLNKPFTSGEILQKARQMCAS
                     WNRRSNLEDSLKMLQEMQAQLVLSERMSAIGQVARGVGHEFGNILQRILGKADLSLNE
                     TDPGKIKENLSLIIQAVDSASVVIRNLQSFSKNSDQKKAVSVAQVFTDTLMLINHDLK
                     KGNIETKVEKGESALVEGNPIELQQVFLNLIINAMHAMPKGGVLSVSIKNDNSRVRIT
                     VSDSGVGISPENLPKIFEYAFTTKGEKGSGLGLSISQKIIEDHRGTIKVASELGKGTT
                     FEITLPGLQR"
     gene            1415..2704
                     /locus_tag="PLH57_01140"
     CDS             1415..2704
                     /locus_tag="PLH57_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK01244.1"
                     /translation="MKQKSDSAEPESNTAFLVVDDDDQARKTIVDYLNAYGFKKIHEA
                     KNGNEALKKLENNKIDFIISDWEMPEPNGLDLLRQIKLDPRFSSIPFIMITSPTSQER
                     FKVEDAALSKVDGYIIKPFRSHILREKIENALVQKEFRERRGVLVVDDDNEVRSLVVT
                     YLRQMGYSPIFEAKDGDEGFAALQLHQKEIAFVVSDWEMPRLTGIDLLAKIRSDAALS
                     EMPFVMITSQTSIERLKVKQAIEGEVDHYLLKPFRSEDLRTKVTYVLNKAKLSIEVGQ
                     LLKTVEMHFKDGRVAEAEKVLHNILVLDPSSVRAHLGLAHAQIKLAPQKGLEKAMNYI
                     RRAITINPQYPQAHIDLAIAFENAMSLEKAIHALKEALKYCSFSPEIHYHLGRLLLRR
                     GRDAEGLAELNKAIELNPAFREAKDLIDAAKDKLGLK"
     gene            2707..3639
                     /locus_tag="PLH57_01145"
     CDS             2707..3639
                     /locus_tag="PLH57_01145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRK01245.1"
                     /translation="MDKARIVIADDDAENVKMLDELVRAEGHLTKTCGDGEAVLHLVR
                     SWSPHLLLLDVDMPKMSGLECAKKVRSQISSDYVGIMMVTAQSDLSTLQNCFDAGADD
                     YLLKPYRSDELRARMKNNLRIRGVHDSLRRMNRKLEEAATIDELTGLRNMRYLSKRLI
                     DEVEHAKKHRVPLSCIMFDMDHFKQVNDQHDHLFGSQVLHEIGQLVMTKLRASDVPAR
                     YGGDEFFILLPGTTLKSAVDVAEQLRKLFETHQFKSGTHATRVTASFGVSGTHHPNDF
                     EVLEARELIRSADSAMYSAKNAGRNRVETYAFIK"
     gene            3682..5934
                     /locus_tag="PLH57_01150"
     CDS             3682..5934
                     /locus_tag="PLH57_01150"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK01246.1"
                     /translation="MLDTLDPKEAAKRRKEKIWIVVAALLFIATTYVEIRLSSLSNRL
                     PFVNSIFFFGLINFNIVLLMILTFLVFRNVGKLFLERRRRILGSRLKTKLVAAFLGFT
                     IIPTIILFLISSFYINSSFDKWFSIKIQNTLQAALEITQTYYKNTSRVAQHLATQSAN
                     DIKEQFGNKLKHTQALTRFVERNREMSAVDGLEVYRDPIEERVVSSSQRDDAEFIPRL
                     PLDILNKVFQGEHVSIVQHIGRADLIRSISPLLSPDDKVYGAVAVTFVIPASLVNRVD
                     EISSVFKDYKDVNPLKYPVKSTYFMILIMMTALIIFASIWIGLYLARELTVPLERLVT
                     ATQKISVGDLDFQVLNPGNDEIAVLTGSFNNMLSDLKTHRFQLEATNQELAEKRGYVE
                     AILNNVTAGVLSADESMTVTTANREAGKLLHRDVKDLIGKKLDSIFSDENVPLVELYK
                     SALEGRIVQKQFALGKEGESLTTLLATATPFHTGVIFVIDDTSAVFKMQREAAWREVA
                     RRIAHEIKNPLTPIKLSAQRLQKKFSSHPSAEAKLLRECTDTIIKNVDSLRDMVNEFS
                     EFARFPTANPTSNNLNEIIRETIRLYEEAERSISFIFEPETRMPLVDVDREQLKRVLI
                     NLLDNAMHALSEGVGQKCALPKRVWVRTHYNEQLNIAVIDVEDNGPGMSDIVRSRLFE
                     PYFSTKTQGTGLGLAIVKRIVSDHHGFIRVYSVPDSGSKFTIELPIRFTTRAQFDEVP
                     LNDSLDSPRA"
     gene            5953..>6895
                     /locus_tag="PLH57_01155"
     CDS             5953..>6895
                     /locus_tag="PLH57_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRK01247.1"
                     /translation="MSRLILVVDDEQEIRHSLSAVFEDEGHRVVSASDSASALEVLTR
                     ESPDVIFLDIWMSVGRGEKQQEGLDLLAKIKAQYPESVVVMMSGHGSIETAVKATKLG
                     AYDFIEKPLSLEKVLVTLQNAFQLRDLSRENRNLRQRVARSKTMIGESAAMVALKAAI
                     RRVASTNSWVLITGENGTGKELVAHTIHAMSSRFEKPFIEINCAAIPEELIESELFGH
                     EKGSFTGATQLKRGKFDLANGGTLFLDEIADMSLKTQAKILRILQEQRFERVGGSQTI
                     EVDVRVIAATNKNLEEEIKANRFREDLYYRLNVVPFRV"
BASE COUNT         2033 a         1456 c         1664 g         1742 t
ORIGIN      
        1 ggaaaaagat tttgaagaag tgtttgaagt ttcttttact actaagagcg ctgaagaagg
       61 aactgggctg ggtttggcaa ttgcacgaag atttattcgg gcctacggcg gcgaattatc
      121 ggtcgaaatg agtgtgccca atgaacgcac ggttttcctt attgagatgc caacaataag
      181 tgaagaatca catcaagagg ttacgaccga tgtctgaagt aaggaaacct aacaatttat
      241 tactggtcgt ggacgacgaa aaggacatcg tacaagccta tatcgatttt ttggtgattg
      301 aaggaaaatc cgaacgcaag agctctcgca gcaaaggtaa cgccaatgaa aatctcgaac
      361 aaaataccga gacctacgag attttaaaag cctataacgg ccccgaagcc cttaaagtgg
      421 tagagggcga acttaaaaat gggcggcgaa ttgcgggcgg tttttttgac gtaaaaatgg
      481 atggtggaat ggacggtctg caaacgatta aagagatctg gaaacgcgat ccgaaggtaa
      541 actgtacgat cgttacggct taccaggata gatctgtgca ggatatcaac cagttgttcg
      601 gcgaaagctt tagagatcaa tgggactatt tgaataagcc ctttaccagc ggagaaattc
      661 ttcagaaggc acgacaaatg tgtgcgtcgt ggaatcgcag aagcaacctg gaagattcgc
      721 taaaaatgct gcaagagatg caagctcaat tggttttgag cgaaagaatg tccgctattg
      781 ggcaagtagc tcgaggagtg ggccacgaat tcggaaatat actgcagaga atacttggta
      841 aggcggacct tagtctaaac gaaactgatc ccggaaagat taaagaaaat ctctcgttaa
      901 taattcaagc ggtagacagt gcctctgtcg taattcgtaa tcttcagtct ttttccaaaa
      961 atagcgatca aaagaaagca gtaagcgtgg cacaggtgtt taccgacact ctcatgctta
     1021 ttaatcatga tttaaaaaag ggcaatattg aaaccaaagt tgaaaagggt gagtccgctc
     1081 tggtcgaggg taatccgatt gagttgcaac aagtgttttt aaatctaatt attaacgcga
     1141 tgcacgcgat gccaaagggc ggagtgttga gcgttagtat aaaaaacgac aattcccgag
     1201 ttcgcattac ggttagcgat tcgggcgtgg ggatttcacc agaaaacctc ccgaaaatat
     1261 ttgaatatgc gttcacaacc aagggcgaaa aaggcagcgg gctaggactg tcaatttctc
     1321 agaagattat cgaagaccat aggggtacaa taaaagtggc aagcgaactg gggaaaggta
     1381 caaccttcga aataactttg ccgggactcc aacgatgaaa caaaaatcag atagtgcaga
     1441 gccagaatct aacactgcgt ttttagtagt cgatgatgac gatcaagcgc gaaaaacgat
     1501 tgtcgactat ttaaatgcct acggtttcaa aaaaattcac gaagcaaaaa acggaaacga
     1561 agcgctcaag aagctggaaa ataataaaat cgattttatt atttcagatt gggaaatgcc
     1621 tgaaccgaat gggttagacc tattgcgcca gatcaaactc gatccgcggt ttagcagtat
     1681 tccgtttatc atgattacaa gccctacgag tcaggaacgc tttaaggttg aagatgccgc
     1741 gttgtcaaag gttgatggct acattattaa accgtttcgt tcacatatcc tacgagagaa
     1801 aatcgaaaac gcccttgtcc aaaaagagtt tcgcgaacgt cgcggggtct tagtggtaga
     1861 tgacgacaac gaagtcagat cgttagtggt aacctatctt agacaaatgg gttattcgcc
     1921 aatttttgaa gctaaggacg gtgacgaagg ctttgcggcg cttcaattac accaaaaaga
     1981 aatcgccttc gtcgtgagcg actgggaaat gcctcgcctt acgggaatcg atcttttggc
     2041 aaagattcgt tcggatgccg cactaagcga gatgccattt gtcatgatta cctctcagac
     2101 ctcgatcgaa cgcttaaaag taaaacaagc aatcgaagga gaagtggacc attatttgtt
     2161 aaaacccttt cggtccgaag atctgcgaac caaagtgact tatgtcttaa acaaagcaaa
     2221 gctgtcgatc gaagtagggc aactgttgaa aacagtagaa atgcacttca aagacggacg
     2281 agtggccgaa gctgagaaag ttcttcacaa cattttagta ttggatccct cgtcagttcg
     2341 ggcacacctc ggattagcgc acgctcaaat taaattggcg ccccaaaaag gtctagaaaa
     2401 ggcaatgaat tacattcgaa gagctataac aattaatcca caatatccgc aggcacacat
     2461 tgacctcgcg atagcctttg aaaatgccat gtcgttagaa aaggccattc acgcgttaaa
     2521 agaagctctg aaatattgta gttttagccc cgaaatacat taccacttgg gccgtctgct
     2581 gttgcggcgc ggtcgagacg ccgaaggtct tgcggaactg aataaggcga tcgaattaaa
     2641 tccagctttt cgcgaagcaa aagatctaat agatgccgcc aaagacaagt tgggactaaa
     2701 gtaaatatgg ataaagcgag aatcgtaatt gctgacgacg atgccgaaaa tgtgaaaatg
     2761 ttagacgaac ttgtacgcgc cgagggacat cttacaaaaa cttgcggtga cggtgaggct
     2821 gtactgcatt tagtgaggtc ttggtccccc catctgttac tactcgacgt tgatatgccg
     2881 aagatgtccg gtctagagtg cgccaagaaa gtacgctctc agattagcag cgactacgta
     2941 ggaatcatga tggtaactgc gcaatcagac ctttctacac ttcaaaattg cttcgacgcc
     3001 ggagccgatg attatctttt gaaaccgtat cgctccgacg agctacgagc gcgaatgaaa
     3061 aataatcttc gtattagagg cgtccacgac tcgctgcgac gcatgaatcg aaagttagaa
     3121 gaggcggcga cgattgatga gctcacggga cttcgaaaca tgcgttactt gtctaagcgt
     3181 ttaattgatg aggtcgaaca cgcaaagaag caccgtgtcc cgctgagctg tataatgttt
     3241 gacatggatc atttcaaaca agtgaatgac caacacgatc acttgttcgg cagtcaggtt
     3301 ttgcacgaaa tcgggcagct tgtgatgacg aagttgagag cgtccgacgt acctgcgagg
     3361 tacggtggtg acgagttctt cattctgctg ccgggcacca cgctaaagtc tgccgttgac
     3421 gtagcggagc agttacgaaa gctgtttgag acacatcaat tcaaatcggg tactcatgca
     3481 acacgagtta cggccagctt tggggtgtcg ggcacccacc atccaaatga tttcgaagtc
     3541 ttagaagcta gggaattgat acgttcagcg gattccgcga tgtatagtgc aaaaaatgcc
     3601 gggcgaaatc gggtagaaac ctacgccttc attaaatagt agcgatcgtg ctataggaga
     3661 caggggagaa ctcctatata aatgttggac acccttgacc ctaaagaagc cgccaagcgg
     3721 cgcaaagaga aaatctggat tgtcgttgcc gcgctgcttt tcatcgcaac gacctatgta
     3781 gagattcgcc tttcgagtct ttcgaatcgt cttcccttcg taaattcaat attctttttc
     3841 ggtcttatca atttcaatat cgtattgctg atgattctta cgtttcttgt atttagaaac
     3901 gtaggtaaac tttttttgga acgccggcgg cgcattctgg gaagccgcct taaaaccaag
     3961 cttgtagctg cttttttggg atttacgatt attcccacca taattctttt tctaatttct
     4021 tcgttctaca ttaattcgag tttcgacaaa tggtttagca ttaagattca aaacacttta
     4081 caggccgcgc tcgaaataac tcagacttac tataaaaata ctagtcgagt ggcccaacat
     4141 cttgcgactc agtctgcaaa cgacatcaaa gaacagttcg ggaataagtt aaaacacact
     4201 caggcactta cgcgctttgt agaacgcaat cgtgaaatgt cggcagtaga cggtttagaa
     4261 gtttatagag acccaatcga ggaacgagtg gtatcttcca gtcagcgcga cgatgctgaa
     4321 tttattccac gcttgccttt ggatatccta aataaagtat ttcaagggga acatgtaagt
     4381 atcgttcaac acattggaag ggcagattta attcgttcca tttcgccact tctatcaccg
     4441 gacgacaagg tgtacggtgc ggtggcggtt acctttgtaa tacccgccag tctggtgaat
     4501 cgcgttgacg aaatttcctc agtattcaag gattacaaag acgtaaatcc gctcaagtat
     4561 ccggttaaat ccacctattt tatgattctc attatgatga ccgcgctcat catcttcgca
     4621 agtatctgga tagggcttta tttggcgcgc gagttgacgg tcccacttga acggctggtt
     4681 actgctactc aaaaaatttc agtgggagat ttagactttc aggttttaaa tcccggtaat
     4741 gacgaaatcg ctgtgttaac tggatcgttc aacaatatgt tgtcggacct aaaaacgcat
     4801 cgttttcagc ttgaagctac caaccaggag ttggccgaaa aacgtggcta cgtcgaagcc
     4861 attctaaata atgtgacagc aggcgtgttg tcggccgatg aatccatgac ggtgacaact
     4921 gcgaatcgcg aggctggcaa actgttgcat cgcgacgtaa aggacttaat cggaaagaaa
     4981 ttggattcca ttttttctga tgaaaatgtg ccactcgttg aactttataa aagcgcgctc
     5041 gaggggcgta tagttcaaaa acagtttgct ctcggaaaag agggagagag tcttacaact
     5101 ttattagcga cagcgactcc ttttcacact ggagttattt tcgtcatcga cgatactagc
     5161 gcagttttta aaatgcagcg tgaggctgct tggcgagagg tcgcacgtcg cattgcgcac
     5221 gaaattaaaa atccactcac tcccattaaa ctttctgcac aacggttaca gaaaaagttc
     5281 tcttcgcatc caagcgcgga agctaaattg ctgcgcgagt gcactgacac aatcattaaa
     5341 aatgtggatt ccctacgaga catggtgaat gaattttcgg agtttgcccg ttttccaacg
     5401 gccaatccaa cttccaataa tttaaacgaa ataattagag aaactatacg tttgtacgaa
     5461 gaagccgaac gttccatttc atttatcttc gaacctgaga ccagaatgcc gttggtggat
     5521 gttgaccgtg agcagttaaa acgcgtgttg atcaatcttc tagataacgc aatgcacgct
     5581 ctttccgaag gagtgggtca aaagtgcgca cttccgaaga gagtatgggt tcgtactcat
     5641 tacaacgagc agttaaacat tgcggtcatc gatgttgaag acaacggtcc aggaatgtcc
     5701 gacatcgttc gaagtagact tttcgagcca tatttttcca ctaaaacaca aggtacggga
     5761 ctggggctcg caatcgttaa gcgtatcgtg agtgaccatc atggatttat tcgagtctac
     5821 tcggtgcccg attctggttc taaattcacg atcgaattac cgatccggtt taccacaagg
     5881 gcccagtttg acgaggtgcc actcaatgac tcgcttgact cacctcgcgc ataaggagat
     5941 gtttttccac aaatgagcag acttattctt gtagtagatg acgagcaaga aattagacac
     6001 agtctttcgg cggtcttcga agacgaaggc catcgcgtag tatcggcctc agatagcgcg
     6061 tctgcccttg aagttttgac gcgggagtct ccggacgtta tttttttaga catttggatg
     6121 agcgtcgggc gtggggaaaa acagcaagaa ggcctggatc ttttggcaaa gatcaaagcg
     6181 cagtatcccg agtcagtggt tgttatgatg tcggggcatg ggtccataga aaccgcggta
     6241 aaagccacca aattaggggc ttacgacttt attgaaaagc cattgtcctt agaaaaggtt
     6301 ctcgtaactc ttcaaaacgc ctttcaactg cgggatttgt cgcgagagaa tcgaaacttg
     6361 cgtcagcgag tcgcgcgttc caaaacaatg atcggtgaaa gtgccgctat ggtcgctcta
     6421 aaagcggcca ttcggagagt tgcctctacg aattcttggg tattgattac gggtgaaaat
     6481 gggaccggta aagaactggt cgctcacaca attcacgcca tgtcctcgcg tttcgaaaag
     6541 ccattcatag aaatcaactg tgcagcgatt ccagaggaac ttattgagtc cgaactattc
     6601 ggccacgaaa aggggtcatt tactggggcg actcagctca agcgcggtaa attcgatttg
     6661 gctaatggtg ggaccttgtt tctcgatgaa attgccgaca tgagtctcaa gacccaagcc
     6721 aagatactaa gaatactaca ggaacaacgg ttcgaaagag tgggtggctc ccaaactatt
     6781 gaagtcgatg ttcgggtaat cgcggcgaca aacaagaatc tcgaagaaga aataaaagcg
     6841 aatcgttttc gcgaggatct ttattatcgg ctcaacgtgg tcccttttcg cgtcc
//
LOCUS       DAOMXO010000028         6854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5818_length_6854_cov_4.496544, whole genome shotgun sequence.
ACCESSION   DAOMXO010000028 DAOMXO010000000
VERSION     DAOMXO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5818_length_6854_cov_4.496544"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PLH57_01160"
     CDS             complement(<1..859)
                     /locus_tag="PLH57_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01248.1"
                     /translation="MKKNQLQYSTVIGVATRLKHCFEALDVKMPDLEIERLSHFINIV
                     YGTRNRAFHDVGHALGVGTGCTAIGQLAALFHDVVYLQVDRSRLNELRSLFGVFDPGD
                     SLELTIPAQETLERDPWRHALVTLFGFKAGQKLGPFTGVNEFLSGWVAVQKLKNLVEA
                     KELIQILACIEATIPFRGEQDAPSASEKLLANLRAASDLLAVNLNVTELEESIGEAVR
                     VSNNDVMGFGLQEPEVFIYNSWALLYEGNPALQNDYYTLIKYREPLLRLESFLGSLNP
                     RKIFLTYKNI"
     gene            1041..2081
                     /locus_tag="PLH57_01165"
     CDS             1041..2081
                     /locus_tag="PLH57_01165"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HRK01249.1"
                     /translation="MKRKSKTKTRAKRKKHKLFNLNFKGHFKWKNPKFLTAVGAALVG
                     FAAGFGHSRAKRKKRQEAMEKVLWALPVLLIFGIVALWNSGAVRYPLTTMSAKLSTMF
                     AKAEKMDRGERIAFWSEAIYKNPALLSVLAPGPKISDTAPIFPNGYDCTTYVETVGAL
                     AKSNNGVDLVDHLISIRYKNGKIGYMTRNHFPEADWIPNNKRSGNLDDITHKVARRSG
                     LTLKYARKEIDKYAWFKKQKNVAASRAIASMSEGETASVKIPYIELESLKSAVTHLPQ
                     GTVINVVRQSLERKPVLITHQGFLVWKKGVPYFRHASSFKEINEVPLVAYVDSLKSMP
                     WRVLGLNFNQYE"
     gene            2139..3122
                     /locus_tag="PLH57_01170"
     CDS             2139..3122
                     /locus_tag="PLH57_01170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRK01250.1"
                     /translation="MDHFFSLTPDRILDSVEDAIGSKKTGVRATGRTFQLNSMENRVY
                     EIELDDESRVVGKFYRPGRWSKDCILEEHEFIRELAAGEVPAVAPIELSNQSTLFETS
                     DGIFFALFPKVRGQIVQELADTELAQIGRLLARLHNVGAAKQAKHRLSLNPKTYGQAS
                     LDFLKSGNFIDIQVLTAYTAAVNELLQKIEPMFQDLPIHRVHGDCHLGNVLWHHTPQG
                     AQAFFLDFDDMVTGPAVQDIWLVVRGRDETAQRQRQVLIESYQTLRSFDIQTLRLIEP
                     LRALRMIHYSAWIARRWNDPIFQRTFPEFGSYRYWQDQLADVHEQLRFIAN"
     gene            complement(3109..3993)
                     /locus_tag="PLH57_01175"
     CDS             complement(3109..3993)
                     /locus_tag="PLH57_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01251.1"
                     /translation="MKRKNFHSLFAFVGLFYGSVVWAGPFSFIFQPYSVGIELDGEFQ
                     GASPNPGQGYFPVPGLTNRQGGATYFGPLASFCVDGTTKAKKVPAVIEYFVKPQIIPS
                     GGGTPENTVYDLVISYGVRAWKVQKSLEKPYLHDWALKDAVLGKGVLAECGGRFVATV
                     RYGVQAIGTYRLRIVGDVDSIAARVEDLRFKHVDETGLASSKGRLDVLKELQDKIDQI
                     EPRIEEIGDDLNFLVRSWRETSLPHLYEIRSGDVDLRLSDELEFFIGSYISGDEVWGD
                     PVSAVVNSYREILPLNSR"
     gene            complement(3993..5312)
                     /locus_tag="PLH57_01180"
     CDS             complement(3993..5312)
                     /locus_tag="PLH57_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK01252.1"
                     /translation="MSKEAFLNKPKHTPEFRKRRAINWLVLGFTYSAMYMGRYNLSFA
                     NKSLSSDYGWDKTQIGAIITAALSIYGFSAIFNGPIADRIGGRRAMLIGASGTVIFNF
                     LFGLGAYLGFLGTGTFLLGYFATVWSLNSYFQSYSALALIKVNSGWFHISERGVFSAI
                     FGSMIQSGRALIFFIGGIVVTVLPWQWVFFIPSMIVAVMALMTYRYVQNGPENCGMPE
                     LDVQDASSGDTEKVTLRYVARKVFTNPVTITIAIAEFCTGFVRHGFEQWFPRYMIEAQ
                     HLTLESPVFQKGAMAVVVAGIAGAFFAGTISDYVFKGRRPPVAFLGYVVQVICLGVIW
                     KAPSLEWVIGAFIVNSFSISVVHSMLSGTASMDFGGKKAAATAAGMFDGMQYVGGAFV
                     GIGMGWLLDKFGWGVWGPSMIGFAAVGGLLMLILWNARPKVKGTGGH"
     gene            complement(5332..6000)
                     /locus_tag="PLH57_01185"
     CDS             complement(5332..6000)
                     /locus_tag="PLH57_01185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRK01253.1"
                     /translation="MQKTYGQNDPKVVDYVEKLLNPEDRVLVDVRRASVENGLPEIQV
                     GPLDALHLEVLTRAVGAKKIVEIGTLGGYSGIAFCRGMGATGKLYTFELQQRNADVAR
                     KNFERADVSQQVEIFVGPALNTLPQIEGFGPFDLVFIDADKSNYCNYLDWAHKNLRKG
                     GVVLADNTFAWGLVVDGAQVDEQDKNSVLGLRAFNRKIAASSSGFRVTMLPTGEGLTL
                     AVKN"
     gene            6119..>6854
                     /locus_tag="PLH57_01190"
     CDS             6119..>6854
                     /locus_tag="PLH57_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01254.1"
                     /translation="MGSIGLQRILISLLVFTANLASATSPSGGPNALPVLTSFPENTL
                     VLVDQTTRRHFYLKEHKVKKFGGLYDSRNFYWVESPEEEIRKQLAGPPNHYAYPGGDV
                     RFSEDRQTLEYRHLNSLEWYPSLLKFSQPNAFLFGATDFKGRIIARIEHPIDGKEYLF
                     ISKKRTPTNYQPMNTLFHWQSPIPGEIAEQVPNPHGEGFFTLLTNGALIYARPFPRWP
                     ESVQTKVVLPPGSVQSMVAAHGKLFAV"
BASE COUNT         1869 a         1789 c         1510 g         1686 t
ORIGIN      
        1 gaatgttttt ataggtcaaa aatatttttc tcggattaag gctgccaaga aaggactcta
       61 aacgaagcag cggttctcgg tacttgatca acgtataata gtcgttttgc agcgcaggat
      121 tgccttcgta gagtaatgcc caggagttgt agataaatac ctctggttct tgaagtccga
      181 accccatgac gtcattgttt gatacccgca cggcttcgcc gatgctttct tcgagttccg
      241 tcacatttag gttcactgca agtagatcag aagcagccct taggttggcg agtagctttt
      301 cggaagcact tggcgcgtct tgttcgcctc gaaagggaat ggtcgcttcg atgcaggcga
      361 gaatttgtat aagttctttt gcttctacca ggttctttag cttttgaact gcgacccagc
      421 cgctcagaaa ttcgttgacg cctgtgaagg ggccaagttt ttggcctgcc ttaaacccaa
      481 ataaggtaac aagtgcatgg cgccacgggt cgcgctcaag cgtttcttga gccggaatag
      541 ttagctccag tgaatcaccc ggatcgaaca caccaaagag ggagcgcagt tcattcagtc
      601 tgctgcgatc cacctgtaga tacactacgt cgtgaaaaag cgctgcgagt tggccgatcg
      661 cagtacaacc ggtaccgacc cccaatgcat gaccaacatc gtgaaacgcg cgattgcgag
      721 tcccgtaaac aatgttaata aaatgactta gacgctcgat ttctaaatcg ggcattttaa
      781 catcgagcgc ttcgaagcag tgtttaagtc tcgtcgccac tccgatgaca gttgaatact
      841 gcagttggtt ttttttcatg gggctcccgt tcctcatttt tcgggattaa tttgaggaat
      901 ctttattttt gagtgggtcc cggctgctaa gttggtctaa accaaattcc actaggcaga
      961 ctttgcccac tcaatactat agctattacc ttcgattctt tttactcaat ttttccaagg
     1021 tctttgccga tgtagagggc atgaaaagaa aatcaaaaac caaaacccgg gcaaaacgta
     1081 agaaacacaa attattcaat ttgaatttta agggtcattt caaatggaaa aaccctaagt
     1141 tccttaccgc agtaggtgcg gcattggtgg gatttgcggc cggctttggc cactctcgcg
     1201 ctaaacgaaa aaagcgccag gaagcgatgg aaaaggttct ctgggcactt cccgtactgc
     1261 ttatcttcgg aatcgttgca ctgtggaata gcggtgcagt tcgatatcca ctgaccacaa
     1321 tgtcagcaaa actttcaaca atgttcgcca aagctgaaaa aatggatcgt ggcgagcgaa
     1381 tcgcgttttg gagtgaggca atttacaaaa accctgcgtt actttcggta cttgcaccgg
     1441 gtccaaagat tagcgacacc gcaccaatat ttccaaacgg ctacgattgc acgacttacg
     1501 tcgaaacggt cggtgcgctc gcaaaaagca ataacggagt cgacttggtc gaccacctaa
     1561 tttccattcg atacaaaaac ggaaaaattg gctacatgac ccgcaatcac tttccagaag
     1621 ctgattggat ccccaacaat aaacgatctg gaaatctaga cgacatcacg cacaaggttg
     1681 cgcgccgatc cggcttaact ctcaaatatg ctcgcaagga aatcgacaag tatgcctggt
     1741 ttaaaaaaca gaaaaatgta gccgcttctc gagctattgc ttcgatgtca gaaggtgaaa
     1801 cggcttcagt taagattcct tatatcgaat tagaatctct aaaaagtgca gtgacccatt
     1861 tacctcaggg cacagttata aacgtagtgc gccaaagttt agagcgaaaa ccagtgctca
     1921 ttacccacca aggttttttg gtctggaaaa agggcgtccc ttattttcgc catgcttcga
     1981 gtttcaagga aatcaacgaa gtcccattgg tggcttacgt agacagcttg aagtcaatgc
     2041 cttggcgcgt ccttggcttg aacttcaatc aatacgaata aaatttcctc gaactcccga
     2101 catgcattct tgagttcacc gtgctagctt tggcaggcat ggatcatttt ttttctctta
     2161 ctccagatcg aatactggac tcggttgaag acgctatagg ttcaaaaaaa accggggtgc
     2221 gcgcaaccgg ccggaccttt cagctaaaca gcatggaaaa tcgggtttac gaaattgaac
     2281 tcgacgacga atctcgagta gtcggtaagt tttatcgacc gggtcgatgg tctaaagatt
     2341 gtatattgga agaacacgaa tttattcgcg aactcgcagc aggtgaagtt cccgccgtcg
     2401 ctcccatcga actttcgaac caatctactc ttttcgaaac ctcagacgga atattttttg
     2461 cgctatttcc aaaagtgcgc ggtcagatcg tacaagaatt ggccgacacc gaactcgctc
     2521 aaattgggcg tctactcgca cgacttcaca atgtcggcgc ggcaaaacaa gcaaaacatc
     2581 ggcttagctt aaatccaaag acctacggac aggcttctct cgatttccta aaaagtggca
     2641 attttatcga cattcaagtt ctcactgcct acacggccgc cgtaaacgag ctgcttcaaa
     2701 agatcgaacc catgtttcaa gaccttccca ttcatcgggt tcacggtgat tgccatttgg
     2761 gtaatgttct ttggcatcac accccacaag gggctcaagc tttttttcta gatttcgacg
     2821 acatggttac ggggcccgct gttcaagaca tttggttggt ggttcggggc cgcgacgaga
     2881 ccgcacaacg ccaacgacag gtattgatcg aaagttacca aacccttcga agtttcgata
     2941 ttcagactct tcgccttatc gaaccacttc gagcgttgcg catgattcac tactcagcat
     3001 ggatcgcccg acgctggaac gacccaatat ttcaacgcac ttttccggaa ttcggttcct
     3061 accgctattg gcaggaccag cttgccgatg tgcatgaaca acttcggttt atcgcgaatt
     3121 aagtggcaga atttcccgat aggaattcac gaccgccgat actgggtcac cccaaacttc
     3181 atcgccactt atataagatc cgataaaaaa ctctagttcg tcggaaagtc gcaagtcgac
     3241 atctccacta cggatttcat acaaatgcgg taagctggtt tcccgccaag agcgcaccaa
     3301 aaaattaaga tcgtcgccta tttcctcaat gcgcggctca atttggtcga ttttgtcctg
     3361 aagctccttc agaacatcta aacgaccttt gctcgaagca agcccagttt catcgacgtg
     3421 tttgaaacgc aaatcttcca ctcttgctgc gatggaatca acatctccta cgattcgcaa
     3481 tctgtaggtt ccaatcgcct gcacaccata tcgaacggtc gccacgaatc gcccgccaca
     3541 ttcggcgagc acgcccttcc caagtactgc gtcttttaag gcccaatcgt gcaggtacgg
     3601 tttctctaaa ctcttttgca ccttccaagc ccgaacgccg taactgatca ctaaatcata
     3661 aacggtattc tcgggcgttc ccccgcccga aggaatgatc tgaggtttta caaaatactc
     3721 gataaccgcg gggacttttt tcgctttagt agtcccgtca acgcagaagc tcgccagcgg
     3781 accgaaataa gtcgcaccac cctggcgatt ggtaagcccg ggaaccggaa aatatccttg
     3841 gccagggttg ggcgacgcac cctgaaattc tccgtcgagt tcaatgccta cggagtacgg
     3901 ttgaaatata aaactgaagg gtccggccca aaccaccgaa ccgtaaaaaa gaccgacaaa
     3961 tgcaaaaagc gagtgaaagt tttttcgttt catcagtgtc cccctgtgcc ttttactttg
     4021 ggccgagcgt tccacaagat caacattaac aatccgccta cggccgcgaa tccaatcata
     4081 cttggtcccc aaacgcccca accaaacttg tccaacagcc agcccattcc gattcctacg
     4141 aaagccccac ccacatactg cataccgtca aacataccag cagcagttgc agcggctttc
     4201 ttgccaccga agtccattga agcagtaccc gagagcatgg aatgcacaac gctaatcgaa
     4261 aaggagttca caataaaggc cccgataacc cactcaagac tcggtgcctt ccaaatgact
     4321 cccaaacaaa tcacctgtac aacataacct aaaaatgcca ctggcgggcg acgccccttg
     4381 aacacgtaat ctgaaatagt tcccgcaaaa aacgcaccgg cgatacctgc cacaacgaca
     4441 gccatggccc ccttttgaaa cactggggat tcaagcgtta gatgttgagc ttcgatcatg
     4501 tagcgaggaa accattgctc gaaaccgtga cgcacgaagc cagtgcaaaa ctctgcaatg
     4561 gcaattgtaa tcgtcaccgg attggtaaac actttgcgcg ccacgtacct taaagtaact
     4621 ttttcggtgt cgccactgga agcgtcttga acgtcaagtt ctggcattcc acaattttcc
     4681 ggcccgtttt gaacgtatcg ataggtcatc aaagccatga ccgcaacgat catcgaagga
     4741 atgaaaaaca cccattgcca gggcaatact gtaacgacga ttccgccaat aaagaaaata
     4801 agtgcacgcc cgctttggat cattgaacca aagatggcgg aaaatactcc acgctcgctg
     4861 atatgaaacc agcctgagtt caccttaatt agagccaagg cactgtagga ctggaagtaa
     4921 gaattaagcg accaaacggt ggcgaaatat cccaaaagga aagtaccagt accaagaaac
     4981 ccgaggtagg cacccaaacc gaacaagaaa ttgaaaatca ccgtacccga ggcgccgatc
     5041 aacattgctc gtcgaccgcc gatacggtct gcgataggtc cgttaaaaat ggcggaaaaa
     5101 ccataaattg aaagagcggc ggtgatgatt gcacctattt gagttttatc ccaaccgtag
     5161 tccgatgaca gcgacttgtt cgcaaacgac aggttgtagc gccccatgta cattgcggaa
     5221 taggtgaacc ccaagaccaa ccagttgatt gctcgtcgct tgcgaaactc cggggtatgt
     5281 ttgggcttat ttaaaaatgc ttcttttgac attgtgctac ctctaaaaaa tttagttctt
     5341 taccgctaac gtcagacctt cgccggtggg caacatagtt acccgaaaac cactcgaaga
     5401 cgctgcgatt ttacgattaa atgctctcaa accgagaaca gaatttttat cttgttcatc
     5461 tacctgggca ccatcgacta ccaaccccca agcaaaggtg ttgtcggcaa ggaccacgcc
     5521 cccttttcgg agatttttgt gggcccaatc taagtaattg cagtaatttg atttgtccgc
     5581 atcgataaac actaaatcga aggggccaaa accttcgatc tgcggcaagg tatttaaggc
     5641 cggacccacg aagatttcca cttgttgact aacgtcggcc cgttcaaagt tttttcgcgc
     5701 tacgtctgca tttcgttgtt gaagttcaaa agtgtataat ttcccagtgg cgcccatacc
     5761 gcgacaaaat gcgataccgg agtaaccgcc cagggttcca atctccacaa tttttttggc
     5821 accgactgcc cgagtaagga cttccaagtg gagagcatca agcggcccaa cctgaatctc
     5881 tggtaatccg ttttcgactg aagcacgcct gacatctacc aatacccgat cttcgggatt
     5941 caggagcttt tccacgtaat cgacgacttt gggatcgttt tgtccgtagg ttttctgcat
     6001 gcgcaccctc gctatttgtg aaccacaggg atgtcctgcc gcagcactaa agtcaagcat
     6061 cggattctaa cttgcgccgc attaatttac tgtgtaagac ccgccaacgg ggagcgcgat
     6121 ggggagcatt gggttacaga gaatattgat ctcattactg gtcttcacgg ctaatctcgc
     6181 gtcggcgact agcccctcag gcgggccaaa tgccctaccc gttttgacct cttttcccga
     6241 aaacacgctc gtcttggttg atcagaccac tcgacgccat ttttatttaa aagagcataa
     6301 agttaagaag tttggcggtc tctatgacag cagaaacttt tactgggtgg aatctccgga
     6361 agaagaaatt cgaaagcaac tggcgggccc cccaaaccat tacgcttacc cgggtgggga
     6421 tgtcagattc agtgaagacc gccagacctt agaataccga cacctcaatt ccttagagtg
     6481 gtacccctct ttattgaaat tttcccagcc aaacgccttt ttgttcggag caacggactt
     6541 taaaggtcgc attattgcac gtatcgaaca ccccattgat ggtaaagaat atctctttat
     6601 tagtaagaaa cggactccga caaactatca accaatgaac acgcttttcc attggcagag
     6661 tcccattccc ggagagatcg ccgaacaagt gcccaatccc cacggagaag gtttttttac
     6721 cctgctcacc aacggcgcgc ttatctacgc tcgcccattt ccccgctggc cggaaagcgt
     6781 ccagaccaaa gttgttcttc ctccgggctc cgtacaaagc atggtcgcag cccatggaaa
     6841 actctttgcc gttt
//
LOCUS       DAOMXO010000029         6783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5896_length_6783_cov_4.353300, whole genome shotgun sequence.
ACCESSION   DAOMXO010000029 DAOMXO010000000
VERSION     DAOMXO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5896_length_6783_cov_4.353300"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLH57_01195"
     CDS             complement(<1..344)
                     /locus_tag="PLH57_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRK01255.1"
                     /translation="MLQQDLFAGEPAALPTQERRSNLALGARLWALAEDHIYFGTSSW
                     KYPAWKNSIYFDDYQTKVAFDKNCLTEYAKTFPAVGGDFSFYQWPHSSMLREIATQCP
                     TKFRIVLKCTDL"
     gene            complement(344..1645)
                     /gene="hisS"
                     /locus_tag="PLH57_01200"
     CDS             complement(344..1645)
                     /gene="hisS"
                     /locus_tag="PLH57_01200"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRK01256.1"
                     /translation="MTQNISTQPYKGTRDFYPEDCRVRTWMFDKLRTTLGRFGYEEYD
                     GPMLEPFELYASKTSEEIVNEQLYWLVDRGDRKLAVRPEMTPTLARMVAARIQELPKP
                     IRWFSIPNLWRYERPQRGRLREHWQLNVDVFGGSRPNEDLEICQLVHAIFDGFGARSG
                     YEVRLNHRGLTNFIFSDLLKLPAEKHASVGRLLDAAPKMGDSKFLAALAEEQVTGKTA
                     QFLVDLLKPDAAKRLEKDLKNCAAWQELTALLQRLEALGLRSHFRFDPSVMRGFMYYT
                     GLVLEAFDLHPDNNRALFGGGRYDNLVGMFGGPALSGMGFGMGDVTLRNFLEVHQLVP
                     NLQAANGVLVAHLSEAQELDALRLADALRSASEQRVLTTLSAEKPKKIFAMAERLRVQ
                     YVVFVGDSEVTSQTFAMKNILTGDQTLGTVAELSFKLKTKS"
     gene            complement(1745..3253)
                     /locus_tag="PLH57_01205"
     CDS             complement(1745..3253)
                     /locus_tag="PLH57_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01257.1"
                     /translation="MAKLFSPPLLALILTAMFGRNTASAAIFYDQANVRCDEIIASFD
                     ELKRQEFTIKLIDELVFNEQGNATPAILQAITLELRRLVILGSPLEKTADYLAAILAV
                     PQITTPTRTAAHEILMNLPPNSRAEAETLVNLALQWIEHPRLSITTESGDFVRLVRRR
                     VLPGFSSFDGPPSRHQELRKKLFDIVVDSRIDIDTSWEAYAGWLELTVSNGILAPEFH
                     LDLWRLVEHLKTGKPHQKLPPNYLYKLFNHLLTYTGVDLERPRHFVAQVLELAPELVT
                     QIMPRLSRSQVLALIEWEHLPAKTLANMVHTLTEFEHLKAAVTRRTSYDSLILVGVWN
                     LGRSVVAGLTPTQRLALMARSLSKPALDFGSINLVLSTYSELSKPGELSAPVFEKPLA
                     TMTEFLLQRLTNDTESTTVQSDKSYIENMLTLLVNLWVKKGIKSPESIRILSSIARHP
                     QLPVRTAEALGHTLLAIEDLSIDTGPIIKALEQRDPGIVKRLIRQGIDPIAY"
     gene            3397..4482
                     /locus_tag="PLH57_01210"
     CDS             3397..4482
                     /locus_tag="PLH57_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRK01258.1"
                     /translation="MGAVNSSSDQTQSASKRLRGAIVGFGFISGKGHFPAYQARTDVE
                     IVAVADVCIARLEAAKKSNETIRTYNSIDELLAAEKELDFIDISTPPCDHFEIALKAL
                     RAGLHVLCEKPLTASLEQARELLLAARRYRRVVFPCHNYKHAPVVKTVDEIIASGEIG
                     RVHGLTLNTYRTTHAKGVSEWKTDWRRDKKYSGGGIAMDHGSHTFYLTFNWLKSLPTA
                     VTAKMVKISEAWDTEDNFSCVLTFPTGYASAHLSWTAGVRKVIYSIQGSQGAITIDDD
                     DVQVASQKGTRKFSIASHWGDASHVQWFNSMFDQFVDCIRNSDYVNAELKEAYHCIQI
                     IQRAYDSSNQSSLEQQLDTTFEFLNHQ"
     gene            4479..5162
                     /locus_tag="PLH57_01215"
     CDS             4479..5162
                     /locus_tag="PLH57_01215"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRK01259.1"
                     /translation="MKKLVRGIVEFRKNIQPEYRTKFAALALGQSPDTLFIACSDSRV
                     VPNLFASTDPGDLFVVRNVGNLIPCCDESGVSKADESEAAAIEFAVLNLGVSDIVVCG
                     HSECGAMRALVDGREKVPFPNLREWLRHGEPSKKRLKQKNLSVNHVNPLSQANVLEQI
                     EHLKTYPLVRERVAARRLRLHAWWFELSTANVYAYEDDQKQFVLIDEAEAARILGALG
                     SSGSDEAPK"
     gene            complement(5089..6225)
                     /locus_tag="PLH57_01220"
     CDS             complement(5089..6225)
                     /locus_tag="PLH57_01220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HRK01260.1"
                     /translation="MPGDFYPFHVVGRRVLAGESIYNLADVSPFKYTPAFALAMITIA
                     KLSKTLAWWIWCVGSVSLFCYSTLRLVGISSFEKLTPRRQFVIAGLAAVSAWHGIIEH
                     LSYGQADMLLFGLFALSALALTHSSNPQDEPKNNTQSLKAGFWLSLCWISKPPSLVLG
                     SYFLIQRQYRALGWAIVFTLLFLFLPIFYWGPSTYLSLFADWFAVLRDRQPIEFLTGN
                     LNQGFAASMARWTDRPESFGTIFKLSILAALAITGVIWRKYRSLPRSTTFAFSYLLYM
                     CVTPLSWRWITCAWLPVAFMVFSELPIKSRITAGAFLVYAVLTLFLQSLVAKQFGVTE
                     PDWLSGWGLYTVGTLLLFAFAIWALHRNQKTQARRESEQLPLRQ"
     gene            complement(6309..6707)
                     /locus_tag="PLH57_01225"
     CDS             complement(6309..6707)
                     /locus_tag="PLH57_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01261.1"
                     /translation="MLPEDYSPEGIYVATDGDLGLLRHNVQFEKNPRGYSIYVRVTNT
                     GLTATTPMKLRVLKQNKPNDPVNAGEASAYVVLRKDIPIPALDPGATTVLQLEWSKNV
                     YSGATPFRFYLDGKDALSRNNSLSVNIHVP"
BASE COUNT         1748 a         1594 c         1800 g         1641 t
ORIGIN      
        1 attaagtcgg tgcatttaag aactattcgg aacttcgttg gacattgagt ggcgatctct
       61 cgaagcattg aagaatgagg ccactggtaa aaagaaaagt cgccaccgac cgccgggaag
      121 gttttcgcat attccgtcaa acagtttttg tcgaacgcga cttttgtttg gtaatcgtca
      181 aaataaatcg aattcttcca agcggggtac ttccaactcg aagttccgaa atagatatga
      241 tcttccgcca gagcccatag gcgagcacca agggccagat tgctacgtcg ctcttgagtc
      301 ggtagtgccg cgggttcgcc tgcaaaaagg tcttgctgga gcattaggac tttgtcttga
      361 gcttgaaact tagttcggcg accgtcccaa gtgtttggtc cccagtcagg atgtttttca
      421 tcgcgaaggt ttgtgacgtt acttcggaat ctcccacgaa cactacgtat tgaacgcgca
      481 gccgttcggc cattgcaaaa attttcttgg gtttttctgc cgaaagcgtg gtgagtacgc
      541 gctgctccga agcactacga agtgcatcgg ctaggcgcag agcatcgagt tcctgagcct
      601 cggaaaggtg agcgaccaac accccattgg cggcttgaag attcggcacc aattggtgaa
      661 cctctaggaa atttctaagg gttacgtcgc ccatgccaaa acccataccg gagagcgcgg
      721 ggcctccgaa catgccgacc aaattgtcgt aacggccacc gccaaagagc gcgcgattgt
      781 tatcggggtg tagatcaaaa gcttcaagga cgagcccggt gtaatacatg aacccgcgca
      841 tgacagacgg atcaaagcga aaatgggaac gaagtcccag agcttcgagc cgttgtaaga
      901 gagctgtgag ttcttgccat gcagcgcagt tctttaaatc tttctctaac cgtttggcgg
      961 cgtctggttt taacaggtct accaaaaact gagcggtttt cccggtgact tgctcttcgg
     1021 ccagggccgc caaaaattta gagtcaccca ttttaggcgc agcatcgagc aggcgtccga
     1081 ccgatgcgtg tttttctgcc ggaagcttca gcagatcgga gaatataaag ttggtgagcc
     1141 ctcggtggtt gagtcgcact tcgtagccag accttgcacc gaaaccatcg aatattgcgt
     1201 gcacgagctg gcatatttct aggtcttcat ttggtcggct ccccccgaaa acgtctacgt
     1261 tgagctgcca gtgttcgcgc agtcgtccgc gctgagggcg ctcatatcgc cagaggtttg
     1321 gaatggaaaa ccatcgaata ggcttgggaa gttcttggat tcttgccgca accattctcg
     1381 caagggttgg tgtcatttcg gggcgtaccg caagtttgcg gtcgccgcgg tcgactagcc
     1441 agtagagttg ttcgttgacg atttcttcac tcgtcttgga agcgtagagc tcgaagggct
     1501 cgagcatggg cccgtcatat tcttcataac cgaaacgccc gagcgtagtg cgtagtttat
     1561 cgaacatcca agtgcgtacg cggcagtcct cggggtaaaa atcacgagtt cctttatagg
     1621 gctgggttga aatattctgt gtcatttgaa ccaaaagtat gtcggtgttc gagggaaatc
     1681 aatgcagcac gaagggaatg gttcattttt tagtagagcg tgcgggtttt gggggtacaa
     1741 aaatttagta agcgattgga tcaataccct gacgtattaa acgtttaaca ataccgggat
     1801 ctcgctgttc gagggcctta atgatggggc cagtgtcgat cgagagatct tcgatcgcaa
     1861 gtagcgtatg gcccagtgct tccgccgtac ggactggaag ttgggggtgt cgagcgatgc
     1921 tcgagagtat acgaatcgac tccggggatt taattccctt ttttacccat agattgacca
     1981 gcagggtgag catgttttct atgtaggact tgtccgattg aacagtggtg gattcagtgt
     2041 cgtttgttaa ccgctgaagt aagaactcag tcatcgtcgc gaggggtttt tcaaaaacag
     2101 gggcactaag ttcgccaggc tttgatagct cgctataggt cgataacacc agattgatag
     2161 agccaaaatc tagcgccggt ttgctcaagc ttcttgccat gagcgcgagg cgttgggtgg
     2221 gcgtaagacc tgccaccacc gatcttccga ggttccacac ccctactaaa attaaactgt
     2281 cgtaggatgt gcgccgagtg accgccgctt ttaggtgttc aaattccgtg agcgtgtgaa
     2341 ccatgtttgc cagtgtcttg gccggcaaat gctcccattc gatcagcgca agtacttggg
     2401 atcgacttaa tcgaggcatg atttgagtga caagttctgg agctagttcg agcacctgcg
     2461 caacaaaatg acggggtcgt tcgaggtcaa cgccggtgta cgtcaaaaga tgattaaaaa
     2521 gtttgtacaa ataattcggt ggcagtttct ggtgtggctt tcccgtcttc agatgttcga
     2581 ctaatcgcca gagatcaagg tgaaattcgg gagctaaaat tccgttcgaa actgttaatt
     2641 ccagccaacc agcgtaagcc tcccacgagg tgtcgatatc aatgcgcgag tcgaccacaa
     2701 tgtcgaacag cttttttcgc agttcttggt ggcgactcgg tgggccgtcg aaactcgaaa
     2761 accctggcaa cactctacgg cgaacaagcc gcacgaagtc tccggactcg gttgttatcg
     2821 atagccgcgg gtgttcaatc cattggaggg cgaggtttac gagcgtttcg gcctccgcgc
     2881 gactatttgg gggcaagttc attagtattt catgagctgc agtgcgagta ggggttgtga
     2941 tctggggaac tgctaaaatg gccgccagat agtccgcagt tttttcgagc ggtgagccca
     3001 aaataaccaa acggcgaagt tccaatgtta tggcttgtag aatggccggc gtagcgttgc
     3061 cttgttcatt aaaaacgagc tcatcgatca gttttatggt gaattcctgc cgttttaact
     3121 cgtcaaacga agcgataatt tcgtcgcaac gaacattcgc ttgatcataa aaaatggcag
     3181 ccgacgcggt attgcgccca aacattgcgg tcaaaatcag agctaacaag ggggggctga
     3241 atagcttggc cacggtccaa cacctcggtt ctggtggcta ttatctaaaa taacgcgcca
     3301 agtctataaa aacagactaa tttgcttcaa ttaatgttta atgagatgag agtttctaaa
     3361 aaaatatggg tgttagcccc tagggagaac aaaggcatgg gcgcagtgaa ttcgtcgagc
     3421 gatcaaacgc aaagcgctag caagaggctt cgaggagcca ttgtagggtt cggatttatt
     3481 tcaggaaaag gccattttcc tgcctatcag gcccgaacag atgtcgaaat cgtggccgtg
     3541 gccgacgttt gtatcgcgcg cttagaggcc gcaaaaaaaa gcaatgagac tattaggact
     3601 tataactcga tcgacgaact gttagcagcc gaaaaagagc tcgattttat cgatatttcg
     3661 acaccccctt gcgatcattt cgaaatcgct cttaaagcgc ttcgcgccgg tttgcacgtg
     3721 ctttgcgaaa agcctctgac cgctagtctt gagcaggctc gtgaactact tttagcggct
     3781 cgtcgttatc gtcgagtggt atttccatgc cacaattata aacacgctcc agtcgtgaaa
     3841 accgtcgatg agatcatcgc ttcgggtgaa attggccgcg tgcacggact gacactcaac
     3901 acctaccgaa cgacccatgc caagggagtt agtgaatgga aaactgactg gcgtcgtgac
     3961 aaaaagtact cgggcggcgg gatcgcgatg gatcacggga gccacacttt ttaccttacc
     4021 ttcaattggt taaaaagttt gccaaccgct gtgaccgcca agatggtgaa aatctcagaa
     4081 gcttgggaca ccgaagataa cttcagttgc gtgctgacgt ttccgaccgg ttacgcttcg
     4141 gcacatctgt cttggacagc gggcgtgcgc aaggtcatct attcaattca aggtagccaa
     4201 ggcgcgatta caatcgatga tgacgacgtt caggtggcct ctcaaaaagg tacacgtaag
     4261 ttttcgatcg cgtcacattg gggcgatgct agtcacgttc aatggttcaa ttcgatgttt
     4321 gatcaattcg tcgattgtat ccgaaattcc gactacgtaa acgccgagct caaagaggca
     4381 taccactgca ttcagatcat acaacgagcc tacgattcat caaatcaaag ctctttggag
     4441 cagcaactcg acactacgtt tgagtttctt aatcaccaat gaaaaagctt gtacgcggaa
     4501 tcgtagagtt tcgaaaaaat atacagccgg agtatcgcac caaattcgcg gcgctggcgt
     4561 tgggtcaatc acccgatacg ctttttatag cctgttccga tagccgggtg gtgccgaatc
     4621 ttttcgcttc aacagatccc ggggatttgt tcgtggtgcg gaacgtggga aatttaattc
     4681 catgttgtga tgaaagtggc gtttccaagg ccgatgagtc tgaagccgcg gcaatcgagt
     4741 ttgccgtgtt gaatttaggt gtgtctgaca ttgtggtttg tggccactcg gagtgtggtg
     4801 ccatgcgagc cctggtcgac ggccgcgaaa aggttccgtt tccaaatcta cgtgagtggt
     4861 tgcggcacgg cgagccttcg aaaaaacgtc taaaacaaaa gaacctttcg gttaatcatg
     4921 taaacccgct ttcacaagct aatgttttag aacaaatcga acatcttaaa acctatccgc
     4981 tggtgagaga acgcgtcgcg gctcgccgct tgagacttca tgcgtggtgg tttgagttaa
     5041 gtactgcaaa cgtttatgcc tatgaggacg atcaaaaaca gtttgttctc attgacgaag
     5101 cggaagctgc tcggattctc ggcgcgcttg ggtcttctgg ttccgatgaa gcgcccaaat
     5161 agcaaacgca aaaagtagta aagttccaac cgtgtatagg ccccagccgc tcagccagtc
     5221 gggttctgtg acgccgaatt gtttggcgac cagggactgc aaaaacaacg tgagcacggc
     5281 gtaaaccaag aacgcgccgg cggtaattcg cgattttatc ggaagttcgg agaacaccat
     5341 aaatgcgaca ggtagccacg cgcacgtgat ccaccgccaa gataaaggtg tgacacacat
     5401 gtaaagcaga tagctaaacg cgaaagtcgt tgatcgaggc agggaccggt actttcgcca
     5461 gatgacgcca gtgatcgcta gcgctgcaag aattgatagt ttaaaaatgg tgccgaagct
     5521 ctctggacgg tcggtccaac gagccatcga cgcggcaaaa ccctgattca gattgcccgt
     5581 cagaaattcg atgggttgcc ggtctcgaag cacggcgaac cagtcagcaa aaaggcttag
     5641 ataggtcgaa gggccccaat aaaaaatcgg gagaaataaa aatagaagcg taaaaacaat
     5701 ggcccaacca agcgcgcggt attgccgttg aattaaaaag tagcttccta agaccaacga
     5761 tggaggttta ctaatccagc agagagataa ccaaaagccg gctttgaggg actgggtatt
     5821 gttttttggt tcatcctggg gattcgagga atgagtcaac gcgagggcac tgagagcgaa
     5881 aagtccaaat aacaacatgt cggcttggcc gtaggataag tgttcgataa ttccgtgcca
     5941 ggcgctgacc gccgcgagac cagcaattac aaattgacgt cgcggtgtta atttttcgaa
     6001 actcgaaatt ccaactaatc gcagtgtgga atagcaaaac agagaaaccg aacccacaca
     6061 ccaaatccac caggcaagcg ttttagaaag cttagcgatt gtgatcatcg ccaaagcaaa
     6121 agctggggtg tatttaaacg gcgaaacgtc tgccaaattg tagatgctct cgcctgcaag
     6181 cactcgacga ccgactacgt gaaaaggata gaaatcgccc ggcactagtc cttccacgcg
     6241 aacgcttaaa ataccaaagc gaacgcagag ataaaccccg agcgctagaa gtgaaaaccg
     6301 tactacgttt agggaacgtg aatattgacg gataatgaat tatttcgact cagggcgtcc
     6361 tttccgtcaa gataaaaccg gaacggagta gctcctgaat acacgttttt ggaccattca
     6421 agctgaagaa ctgtggtggc ccctgggtct agtgcgggaa tcgggatgtc tttacgcaag
     6481 acgacatacg cagacgcttc gccagcattg accggatcgt tgggcttatt ttgttttaat
     6541 actctgagtt tcattggtgt tgtggcagtt aggccggtgt tagtgacgcg cacgtaaatc
     6601 gaataccccc tcgggttttt ttcgaactgc acgttgtgtc ttaaaagccc taagtctcca
     6661 tcggttgcaa cgtagatacc ctcgggactg tagtcctccg ggagcaccga gcctctagtt
     6721 tgcaccgaac gatgtagggc gcgccaggtt gatcaccacg tcgcagccgg cctggccgat
     6781 cag
//
LOCUS       DAOMXO010000030         6753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_5938_length_6753_cov_4.735294, whole genome shotgun sequence.
ACCESSION   DAOMXO010000030 DAOMXO010000000
VERSION     DAOMXO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5938_length_6753_cov_4.735294"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="PLH57_01230"
     CDS             complement(<1..1574)
                     /locus_tag="PLH57_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRK01262.1"
                     /translation="MSSKTQSFPTITQTFLNRVRSAPDKKAFFSKVGTEWKATTWKDY
                     HGLVREIALGIQQLGLSAGEKVALISTTRIEWAACDMAILANCGVTVPVYPSNTHDDV
                     EFILQHSESAIVFVEDAKQLDKVLHARSKLTKLKKIVIFDSVGAHDLEKNGVLTLSAL
                     RALGADKGDKDQFEKGLLAAKPTDVFTICYTSGTTGVPKGVVLTYDSLSSMLEDVGNR
                     LGRWVSDEDTVLSFLPASHIFGRIESMAAYHFGWTTYYAESIEKILLNLGEVGPTILF
                     AVPRIFEKAYNRIKANIDESSSTKRKLFAWASQVGRAYYGKIWEGKRPTSVEFAKYQV
                     AKKLVFSKVYARFGGRIKFCIAGGAPLPKEIGEFMRIVGISILEGYGLTETCAAVTVN
                     TPDEIKFGSIGKPLPEVAIKIADDGEILIKSRKVFKEYYKNPEATKEALDSNGWFHTG
                     DIGVIDGEGFVKITDRKKDIIVTSGGKNVAPQKIENLAKTHKYISQFVVIGDRRNYLS
                     ALVVLEKDEAVKFAKE"
     gene            complement(1646..2134)
                     /gene="smpB"
                     /locus_tag="PLH57_01235"
                     /pseudo
     CDS             complement(1646..2134)
                     /gene="smpB"
                     /locus_tag="PLH57_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003353509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
     gene            complement(2140..2793)
                     /locus_tag="PLH57_01240"
     CDS             complement(2140..2793)
                     /locus_tag="PLH57_01240"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRK01263.1"
                     /translation="MTMIKNALGSFRSNVITGALVLIPFAAVVWILLSLWAWIMGLSD
                     LVPTALHPRAWLGLESELANKGIDFAVTLLVLAILIAVVWGAGVVSRHYLGKQLLEGF
                     AKFLSRVPVLSTVYSTLQQLLETFGSAKAKNFRRVVAVEYPRKGLYTLAFVTGERNSG
                     PGGESGKFLTIYVPTTPNPTSGFYLTVSADEVRESKVSVEEALKEIISMGIVHKHGT"
     gene            complement(2808..4163)
                     /locus_tag="PLH57_01245"
     CDS             complement(2808..4163)
                     /locus_tag="PLH57_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01264.1"
                     /translation="MSTRFWWFGFCLISILTVHSKAFASTGETYGYGSRASALGNTML
                     GGIQDGYSTFYNPAANSANSGLRLSLGVAYAKPSFLEIKDIVVGNTAVAAETGDVLGS
                     VKNDDYLDHLGQTVGASLNFGEKWKLLTVGLTGSLPITRLAYLDTGEAFRPEYFGYRS
                     RTQRPQVYASMSVSPFQNFYMGTGIAISTNLAANTTLYATTNPGSVSYQRFSSTIKPG
                     AAPYFSLYGTPSKLIWGVTARLPNRYTLSIDTNASARLFGTQSGIPLVTNSASTLYYD
                     PLEVDLGMGLHIAPQTFATLEVDWFQYKKYESPTVKVVDKGSGAALLTSISNVPTMRD
                     IFVPKLGLEHRFEKFKARFGYAFRKSPVENNNGPGNLIDPDKHLFSLGAGIDLKEAGM
                     VAQNLILDLHAQYHWLVRKRVTKALGNEVGTLSQSKVGAPGYDIGGKIYSAGLSFSMA
                     F"
     gene            complement(4160..4996)
                     /gene="rsmA"
                     /locus_tag="PLH57_01250"
     CDS             complement(4160..4996)
                     /gene="rsmA"
                     /locus_tag="PLH57_01250"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRK01265.1"
                     /translation="MKSRQQVLGQNFLKHTPTIEKIITSVRHRLEQDRKRGIAPKAIL
                     EVGPGALALTKTLAKIAADEKLDFVIVERDRFLEEGIRTGLPQAEVHFFDAATEKLAE
                     LFDQLRSRGSTPILFVSNLPYSAASQILAAVCKRIELLSSAVVMVQKEMAQRIIAPEK
                     SEHRGSFSLFMQSYFAPKILFDVPPGAFSPPPKVMSTVLDLVPLEKTVLDRIEQPLEF
                     EKFCKKLFSQRRKMIRNSLNLPKTGPLPDAFAKLGINGTERAEVLDFETVLQLYLASG
                     GK"
     gene            complement(4998..6086)
                     /gene="tsaD"
                     /locus_tag="PLH57_01255"
     CDS             complement(4998..6086)
                     /gene="tsaD"
                     /locus_tag="PLH57_01255"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRK01266.1"
                     /translation="MSNKKLLAIETSCDECSASVVEQADPWVKPLSNVIFSQIELHRR
                     FGGVVPEVASRNHLDMLFPVIEEALIKAGVGFKDLDGVAVTQRPGLIGALLVGVSAAK
                     AIAYALDKPFIPVNHLEGHVSSLFLAHQGSFPALTREHLPLVICLVSGGHTQLYLMKE
                     LPPQNLGLKLLGESRDDAAGEAFDKGAKLLGLPYPGGRAIDEHAKKGNSKAFALPRPM
                     LGDNLEFSFSGLKTALFTELKNQGYEPSARISTKPAKLPTGKALDDFCAVLQESIIEA
                     LFRKTQLAIQKTGARAVAVVGGVSANSRLRSRFTHEISVPFISVPLEFCTDNAAMIGA
                     AGMYRLLNGSFLQGPQLLSATAYSNSEG"
     gene            complement(6108..>6753)
                     /locus_tag="PLH57_01260"
     CDS             complement(6108..>6753)
                     /locus_tag="PLH57_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRK01267.1"
                     /translation="SNPMDQLLLRSKKRIAELNAESDAEIFGDAVPAKNKHVRAEKKV
                     RNTTTPGQVLQTFAQCAQCHSKQHEFVSKTRHSSAYLTLMKVGQNRNLECLQCHTLGQ
                     GMPSGYSHVDRLVIGTDGSQLNVEKFASELPQMSVAELRKVKRALINVQCESCHSVAP
                     THARKSGGTNISPKPGAATCLACHTSDRAPGWYRDGKLDQKLFAEKLESVSCPR"
BASE COUNT         1735 a         1660 c         1537 g         1821 t
ORIGIN      
        1 tgttccttag caaatttaac tgcttcgtct ttttcgagca ctacgagagc actaagatag
       61 ttacgacggt cgccgatcac cacaaattga ctaatgtact tgtgggtttt ggcgaggttc
      121 tcaattttct gaggggcgac atttttacca ccgctagtta cgataatatc tttcttgcgg
      181 tcggtaattt ttacgaaccc ctcaccatcg atcacgccaa tgtcgccggt gtgaaaccag
      241 ccgttcgaat cgagggcttc tttggtagcc tctgggtttt tatagtactc tttgaaaact
      301 tttcggctct taatcagtat ttcaccatcg tctgcaatct taattgcaac ttcaggaagt
      361 ggttttccga ttgatccaaa ctttatttcg tcgggcgtgt taacggtgac cgctgcacaa
      421 gtttccgtta gtccgtaacc ctcgagaatc gaaatgccta caatgcgcat gaattctccg
      481 atttcctttg gcaaaggagc tccaccggca atgcaaaact tgatacggcc accaaaacga
      541 gcgtatacct ttgaaaaaac caatttcttg gctacttggt atttggcaaa ctctaccgac
      601 gttggtcgct tgccttccca aatttttccg tagtacgcac gacctacctg gctggcccaa
      661 gcaaacagtt ttcttttggt agatgacgat tcgtcaatgt tggctttgat tcgattgtag
      721 gccttttcga aaatgcgagg caccgcaaac aatatcgttg gaccgacctc accaagatta
      781 agaagaattt tttcgatact ttccgcgtaa taggttgtcc aaccaaagtg gtaggcagcc
      841 atgctctcga tacgtccaaa tatgtgcgat gccggcagaa aagataaaac cgtatcttcg
      901 tcgctcaccc accgccccag acggttgcct acgtcttcca acatgcttga aagactatcg
      961 taggtgagaa ccacgccctt tggcactccg gtggttcccg aagtataaca aatcgtaaac
     1021 acatcagtgg gtttagcggc taataggcct ttttcgaact gatccttgtc tcctttatcg
     1081 gcgccaaggg cccgcagagc tgaaagcgtt agaactccgt ttttttccaa atcgtgggca
     1141 cccactgaat cgaaaataac gatctttttc aattttgtta gttttgatct agcgtgcaaa
     1201 accttatcga gttgcttcgc gtcttcaaca aatacgattg cggactccga atgctggaga
     1261 ataaactcga catcgtcatg ggtattcgac ggataaacag gcaccgtcac accgcaattt
     1321 gcaagtatcg ccatatcgca ggctgcccat tcgattcgcg tagtcgaaat taacgctacc
     1381 ttttcacctg ccgaaagacc caattgttga ataccaagag caatttcacg caccagaccg
     1441 tggtaatctt tccaggtagt agctttccat tccgtaccga ccttactaaa gaaggccttc
     1501 ttatcagggg cgcttcggac tcgattcaaa aaagtttgag tgatcgtggg aaaggattgg
     1561 gtcttcgagc tcatttttct tgtcctttgt tgaattcaaa agaatctgat tccttgaaca
     1621 gtaaacaata ggaacaatac ttaggcaacg agcgtcgcga acgtttagat gccttgtcca
     1681 tttcacgctt cgcatcgcgt cgtttaatgt cttcgcgccg atcacctttg gttttgcctt
     1741 ttgctaaagc gagctcaacc tttacgcgac ctttgaccca aaatagccgt accggcacca
     1801 gtgtgaagcc tttttctacg actgacccta tgagctttcg aatttcatgt ttatgtaaca
     1861 acagctttct tttgcgacgc ggttcgtggt tgaagcgatt cccgtgcgaa tagggggcga
     1921 tttccatgtt atgtaaaaag gcctcttcgt gctcaatgtt ggcgtaggag tcgctcaaat
     1981 gaacgccgcc ggctcgaatg gattttactt cggtgccact taacaccagt cccgcttcaa
     2041 aacgttcatg aagggcgaaa tcgcgagctg cgtgtttatt gacacaaatt tgagctggta
     2101 cgactttttt ttctgtttgt ttgggcgaag gcatttagct catgtcccgt gtttgtgaac
     2161 aatgcccatg gaaattattt ctttgagcgc ctcttcgacc gatactttac tttcacggac
     2221 ttcgtcggca gataccgtga gataaaagcc tgaggtcggg tttggtgtcg taggtacgta
     2281 gattgttaaa aacttgccag attcgccccc cggcccactg ttgcgttcac ctgtaacaaa
     2341 agccaaagtg tagagtcctt ttcgagggta ttccactgcg actacgcggc ggaagttttt
     2401 tgctttggcg ctgccgaatg tttcgagcaa ttgttgtagc gtagaataaa ctgtgctgag
     2461 cactggaact ctcgaaagaa atttggcaaa tccttcgagt agttgtttac ctaaataatg
     2521 ccgcgacacc acacctgcac cccatacaac ggcaattaaa attgctaaca ctaaaagagt
     2581 caccgcaaag tcgattccct tgttagccaa ctcactttct agtcctaacc aagcgcgcgg
     2641 atgcaacgca gtgggcacta aatccgataa acccatgatc cacgcccaaa gagaaagcag
     2701 tatccaaact actgccgcga aaggaatgag tacgagtgcg ccggtaatta cgttgctacg
     2761 aaaagacccg agtgcatttt tgatcatggt cataggagta aattccctca aaaggccatc
     2821 gaaaatgaaa gtccggcgct ataaattttg ccacctatgt cgtaccctgg tgcgcccacc
     2881 tttgactgac taagcgttcc gacttcattt cctaaagcct tggtaacacg tttgcgtacc
     2941 aaccagtggt attgtgcgtg aaggtctaaa attaaattct gagcaaccat tccggcttct
     3001 tttagatcga taccagcgcc caaactgaag agatgcttgt cggggtcaat taagttgccc
     3061 ggaccattgt tattttcaac gggcgacttt ctaaaagcat agccaaatcg cgctttgaat
     3121 ttttcaaacc gatgttcaag gcccagtttc ggaacaaata tatctcgcat cgtgggcaca
     3181 ttagaaatcg aagtcaatag agctgcgccg gatcctttat ccaccacttt gaccgttggc
     3241 gactcgtact ttttgtattg aaaccaatca acttctagcg tggcaaaagt ctgcggggcg
     3301 atgtgtaagc ccattccgag gtccacttca agcggatcat aatacagagt agatgcacta
     3361 ttggtcacta agggtatacc actttgggta ccaaacaatc gggcactcgc attggtatcg
     3421 atgctcaaag tataacgatt gggtagacgc gccgtcactc cccaaatcaa ctttgatggc
     3481 gtgccataga gcgaaaaata aggcgcggcc cccggcttta tagttgagct gaagcgctga
     3541 taactgaccg aaccaggatt ggtcgtagcg tatagtgtcg tattcgcagc taggttggta
     3601 gatatcgcga tgccggtgcc catataaaaa ttttgaaacg gcgacacact catcgacgca
     3661 tatacttgtg gacgttgggt tcgagatcga tagccaaaat actcaggtct aaatgcttca
     3721 ccagtgtcta agtaagccag ccgagtaatt gggagcgaac cagtcaaacc gacggtcaat
     3781 agtttccatt tttcgccgaa atttaaacta gcgcctactg tttgcccaag atggtccaaa
     3841 taatcatcgt tttttacact gccgagtaca tccccagttt cggcggcgac cgcagtgttt
     3901 ccgaccacaa tatctttgat ttcgagaaaa ctcggtttgg cgtaggccac gccaaggctc
     3961 aaacgcagac ccgaattagc actattggcc gctggattat aaaaggtcga atacccgtct
     4021 tgaatgccac caagcatcgt attgccgagt gccgacgcgc gcgaaccgta accataggtt
     4081 tcaccggttg aagcaaacgc ttttgaatgc accgtcagaa tcgaaattag acagaaaccg
     4141 aaccaccaaa aacgcgtgct cattttcctc cagacgccaa ataaagctgc aaaacggttt
     4201 cgaaatcgag tacttccgct cgttccgtac cattgatgcc aagctttgca aaggcgtccg
     4261 gcagtggccc agttttgggt aaatttaatg aattccggat catttttctt cgctgactaa
     4321 agagtttttt acagaatttt tcgaactcta atggctgttc gattcgatcg agtacagttt
     4381 tttccagagg taccagatct aaaacggtac tcataacttt tggcgggggt gaaaacgcgc
     4441 cgggtggtac atcgaataaa attttagggg caaaataact ttgcataaaa agactgaaag
     4501 acccccgatg ttccgacttt tcgggggcaa ttattcgctg tgccatttcc ttttgcacca
     4561 ttacgacggc ggaactcaaa agttcgattc gtttgcaaac cgccgcaaga atttgtgacg
     4621 ctgctgaata aggcaaattg gatacaaata gaattggggt tgatcctctc gatctgagtt
     4681 gatcgaagag ctccgcaagc ttttcagtgg ctgcgtcaaa gaaatgaact tcggcctgtg
     4741 gcaaaccggt acgaatcccc tcttccaaaa atcgatcacg ttcgacaata acaaaatcta
     4801 acttttcatc ggccgcaatt ttagccaagg ttttcgttag tgccaacgct ccagggccta
     4861 cctctaaaat ggctttgggc gcaattccgc gcttgcggtc ttgctcgaga cgatggcgaa
     4921 ctgaagtaat aattttttca atcgtgggtg tgtgctttag aaagttctga cctaaaactt
     4981 gttggcgaga tttcacgtta accctcggaa ttagaatatg ccgtagccga cagcaattgg
     5041 ggcccttgca aaaagctacc gtttaaaagt cggtacatcc ctgctgcgcc aatcattgct
     5101 gcattgtccg tacaaaattc taatggcact gaaatgaatg gaactgaaat ttcgtgagta
     5161 aaacgactgc gcaacctact gttagccgaa acccctccga caacggccac cgcacgggcg
     5221 cccgtttttt gaatcgcgag ctgcgttttc ctgaatagcg cttcgatgat cgattcctgt
     5281 aaaaccgcac aaaaatcgtc tagagccttt ccagttggca gttttgcagg cttagtagaa
     5341 attcgagcgc taggttcata tccttgattc tttaattcgg taaataaggc ggtttttaac
     5401 ccgctaaaac tgaattccaa attgtccccc aacatgggac gcggaagagc aaacgccttt
     5461 gagttacctt tttttgcatg ttcgtcgatg gcgcggccac caggataagg aagccctaac
     5521 aacttagctc ctttatcgaa tgcttcgccc gctgcgtcgt cgcgcgattc gcccaaaagc
     5581 tttagcccaa gattttgcgg cggaagttct ttcattaagt atagttgggt gtgccccccg
     5641 ctcaccagac agataacaag gggaagatgt tctcgagtaa gtgccggaaa actaccttgg
     5701 tgcgctaaaa aaagcgaact tacatgcccc tccaagtgat tcaccggaat aaagggtttg
     5761 tccaatgcgt aggcaattgc ttttgctgca gagaccccta ccaaaagggc tccgatcaaa
     5821 cccgggcgct gtgtgactgc tacgccgtcg aggtctttaa atccgacacc ggctttaatt
     5881 aaggcttctt cgatgactgg aaacaacatg tccaaatgat ttcgggaagc aacctcgggc
     5941 accacgccgc caaaacgcct gtgtagttcg atctgcgaaa aaataacatt tgaaagcggt
     6001 ttaacccaag ggtcggcctg ttccaccact gaggctgaac attcgtcaca cgaagtttct
     6061 atagcgagaa gctttttgtt cgacatcaga ttttacatac cacagactca acgcggacag
     6121 gaaacgctct caagcttttc agcaaagagt ttctgatcca acttcccatc gcggtaccaa
     6181 ccgggggccc ggtccgaagt atgacaggct agacaggtag cggctcccgg cttcgggcta
     6241 atgttcgtac ccccggactt gcgggcatgg gtgggcgcca ctgaatgaca gctttcgcat
     6301 tgcacattta taagcgcccg ctttacttta cgaagctcgg ctaccgacat ttgcggtagt
     6361 tcgctggcaa atttttcaac atttaactga gaaccgtcgg tgccgatcac taggcgatca
     6421 acgtgggaat acccggaagg catcccctga ccaagcgtgt ggcactgtaa gcactctaag
     6481 ttcctatttt gacctacttt cataagagtg aggtaggcgc tcgagtgacg ggttttggac
     6541 acaaattcgt gttgtttgct atggcactgg gcacactgcg caaatgtttg gagcacctgc
     6601 cctggagtgg tcgtgtttcg tacttttttt tctgcccgca cgtgtttatt ttttgccggt
     6661 accgcgtcgc cgaaaatttc tgcatcactt tcggcgttga gttcggcaat tcgttttttg
     6721 gaacgcaaaa gcagttgatc cataggattc gat
//
LOCUS       DAOMXO010000031         6620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6113_length_6620_cov_4.796954, whole genome shotgun sequence.
ACCESSION   DAOMXO010000031 DAOMXO010000000
VERSION     DAOMXO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6113_length_6620_cov_4.796954"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PLH57_01265"
     CDS             complement(<1..1035)
                     /locus_tag="PLH57_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRK01268.1"
                     /translation="MQNHLVNERRARVLALKQRLADKVLVIDGAMGTAIQERNLTAED
                     FGGEDLDGCNENLVLTRPDVIQGVHEGYLKSGADIVETNTFGATPLVLAEYGLQHKAV
                     EINTAAVRLARAACEKYATTDWPRFVAGSIGPTTKALSVTGGATFEELLDTFYVQVKA
                     LYDAGVDYFLLETCQDMRNVKAGLLAIERLFTTISKEDRIPVAVSLTIEPMGTMLGGQ
                     NVEAALASLEHTDLLYIGLNCATGPEFMTDHIRTLSKYASMPVACVPNAGLPDENGKY
                     LETPGMVAAALKRFGDNTWINVVGGCCGTHSGHIAELRKLADSLKPRTPVKTRRSVLS
                     GVDPLEITDEM"
     gene            1111..2838
                     /locus_tag="PLH57_01270"
     CDS             1111..2838
                     /locus_tag="PLH57_01270"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRK01269.1"
                     /translation="MKISKYFYFTSKSVSQEDEAISYKLMLRAGMLLKHGAGLYSYLP
                     MAQKALEKVKRVIREELDRDGNVELLMPFVQPAALWKESGRWEDMGPEMARFKDRKKN
                     DYALSPTHEEVITDVFRSQAKSYKDLPISLYQIATKFRDEPRPRFGLLRGREFIMKDG
                     YSFHETQECLQKTYDAYFKAYCRIVERLGLKYRVVQADTGNMGGSMSHEFHVLAERGE
                     DEILFCDAIGLAANAEMAPLPKNAHFTKSNGTGGGVEKVGTPGATKLEDLERTLKVSM
                     VDMIKSVAYELVVPGVGESRPVVVFIRGDLPVSETKLKKYLDRGELRPLSVDTAKKFG
                     LTLGFMGPSNSPKTGVIYLFDHSLSQPGKFWVSGANETDFHFKNFQEGRDFQVHESKD
                     LALAKKGDRCDETNVYESARGIEIGHIFQLGKKYTEAMKVSLHGSDGKPMVPTMGCYG
                     IGVSRLMASAIEQNNDERGMIWPKAIAPYQVHLICSNMKNDAIRQAAEAFYGELGEMG
                     FEVLFDDRELQFGAKITDAELCGMPIIVVIGNAFASSGQFEVIIRQGLIKQQLGKEAT
                     VSLLKSTLL"
     gene            complement(2833..3855)
                     /locus_tag="PLH57_01275"
     CDS             complement(2833..3855)
                     /locus_tag="PLH57_01275"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRK01270.1"
                     /translation="MSTANLLSQEIALWLIPERALKRRWWPLALGLAYVLTIFVLGGL
                     ESKHVIIASLVLLDSYNSRTRTFLKYFTPFILTGMAYDSMRYYYWQGIEGNVHVSEPY
                     FLEKKLFGIESNGTVLTPNEYFERNNWKILDFFAGFAYITFVFEYLGTGFLLLFARQL
                     AVLRTFGWCFFTVNIMGFATYFIYPAAPPWYVAAYGLGPARTDVVASPAGAARFDQLM
                     GTHFFDAMYGMSVDVYGAIPSLHVSYPLLVAWAALSVKRFRTPAIVFFLLMCFSAVYL
                     NHHYIIDVILGILYSLTAFVIVRGIESWLQKRYARSESESDRQINSLKSDSTNRRYNA
                     ETASPS"
     gene            3854..4537
                     /locus_tag="PLH57_01280"
     CDS             3854..4537
                     /locus_tag="PLH57_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01271.1"
                     /translation="MISVLKPQRMLHLRLLQVVFSVVFFGFGPLALATEGASACFDFS
                     KKVADPLRTAPAWKSDRREDVSGRGKVTRHEPGKPKSGYVWGQARGVVRKPIRELLNL
                     LLDHNTTKSSKIREINFSKSSDENYFARHVITFEVTALIFFSAEWTEEWGYAITRGSG
                     MAPERVVISYQKTAGTSHIEHLCGSIVLEMIDANTTDVYAYEEVNANQRNDEDTESGL
                     VGTLLTLRK"
     gene            4543..5124
                     /locus_tag="PLH57_01285"
     CDS             4543..5124
                     /locus_tag="PLH57_01285"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRK01272.1"
                     /translation="MILTKGLQAVYAATIDRSKFREVVSRLVVFVLPLFYWGRQPWWW
                     GLLFVGAGILLRAWAAGYLHKDEEQAAAGPYLLVRHPLYLGSCLLALGLIITLHHWFV
                     TLAIGGVTALTYLHTIRHEEKNLLKRFGESYARYCNETGPLWPRVGGLKKFFAFKSKR
                     KFSFRQYLKNKEYECLLGVVVVLGVLYLGAGSK"
     gene            5121..5897
                     /locus_tag="PLH57_01290"
     CDS             5121..5897
                     /locus_tag="PLH57_01290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK01273.1"
                     /translation="MRLTVVVPTFNEADTIENSLREIERVLGPELSKDTELLIADDGS
                     DPMPELVERLKKEIGFARLELLRNSPPVGKGLSLARAFERAQGRTVGFLDADLSTPAT
                     YILEALKVLEANEADLYIGSRRAMGANVVREQFWLKDVLGDALRVGVNAVIFRLGRKY
                     SDTQCGFKFFKNQVAKVLYCNLVAYDGVTDVEILVRANLLGYRVCERGVEWCDTRESK
                     RSLKRILIGDLKAITRIMWHYGVFEKKQIKDLRRFEKTLI"
     gene            5905..>6620
                     /locus_tag="PLH57_01295"
     CDS             5905..>6620
                     /locus_tag="PLH57_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01274.1"
                     /translation="MFNLKKWSPQEVVLATAIVLIAAIYSYFFIYLPQYWTLRDDYGF
                     ALKWGANPDKVVDYGWAYLKGIMTHGRFQPMDVLNRVIHYGILPIDARYFRISFFISV
                     FISVVSFVGILRVFGCARDQILLAVLLLVSNHSLKEMLTTMTVSERIGAMYFMVALWA
                     YSANWKNSQWWALLIFPLSFFGKESFVVLAPVFAVVAFFQKNKKSNAGLIVALLLMIE
                     ALIFVIFIRNLPKLYTEGLS"
BASE COUNT         1811 a         1401 c         1637 g         1771 t
ORIGIN      
        1 catttcatct gtgatttcca gcggatcgac accagacaaa accgatctcc tggtttttac
       61 aggagtcctt ggcttcagtg agtcggccaa cttgcggagt tctgctatgt gaccagaatg
      121 agttccgcag cagccgccaa caacgtttat ccaagtgtta tcgccgaatc gcttaagggc
      181 ggcggctacc attcccggtg tttcaagata ttttccgttt tcgtcgggga gcccggcatt
      241 aggaacgcag gccactggca tactcgcata ctttgaaagc gtgcgaatgt ggtccgtcat
      301 gaattcgggc cccgttgcac agttgagacc aatataaagc agatcggtgt gctctaacga
      361 cgctaatgcg gcttctacgt tctggccgcc gagcattgtg cccatgggtt cgatagtaag
      421 agaaactgcg acaggtattc ggtcttcttt tgagattgtt gtaaacagtc gttctatcgc
      481 gagtagtcca gcttttacat ttcgcatgtc ctgacaggtt tcgagcaaaa agtaatcaac
      541 tcctgcgtcg tacaaggctt ttacctgcac gtaaaaagta tctaatagtt cttcaaaagt
      601 agctccgccg gtaaccgata gggctttcgt ggtaggtccg attgatccag cgacaaaacg
      661 tggccaatca gtagttgcat acttttcgca agcggctcgg gccaatcgga cggccgcggt
      721 attaatttca actgctttgt gttgtagacc gtattctgcc aatacaagcg gagttgcacc
      781 gaacgtgtta gtttcaacaa tgtcggcccc agacttcaaa taaccttcgt gcaccccttg
      841 aataacatct gggcgagtta aaacgagatt ttcgttacag ccgtcgaggt cttcaccacc
      901 gaaatcttcg gcggtcaaat tacgttcctg aatggccgtg cccatggcac cgtcgatgac
      961 tagtactttg tccgcaagtc gctgtttaag cgctagtaca cgagcccgac gctcattcac
     1021 caaatgattt tgcatacata aagaggcgta tcttgcttaa ctgaaaaact caatttaaaa
     1081 ggattatgcg aataatctaa gagaaaaatt atgaaaattt ccaaatattt ttattttact
     1141 tcgaagtcag tttcccaaga agacgaggcg atttcgtata agctaatgct gcgcgccgga
     1201 atgttactta aacacggtgc aggactttac agctatttgc cgatggcaca gaaggccctc
     1261 gaaaaagtga aacgcgttat tcgcgaggaa cttgaccggg atggtaacgt tgagttgctg
     1321 atgccgtttg ttcaaccagc ggccttgtgg aaagagtccg ggcgatggga ggacatggga
     1381 cctgaaatgg cgcgattcaa agatcgaaag aaaaacgatt atgcgctttc tcccacacac
     1441 gaggaagtaa ttactgacgt ttttagaagc caagcgaagt cctataaaga cctcccaatt
     1501 tctttatatc aaattgcaac gaaatttaga gacgaaccac gaccccgatt tggattactt
     1561 cgcggtcgcg aattcattat gaaagacggc tatagttttc atgagactca agaatgttta
     1621 cagaaaacct acgatgcata tttcaaagcc tattgtagaa ttgtcgaacg ccttgggctg
     1681 aaataccgag tggttcaagc ggacaccgga aacatgggtg gctcgatgag ccacgaattt
     1741 catgtattag cggaacgagg agaggacgaa atattatttt gtgatgcgat tggacttgcc
     1801 gcgaatgcgg agatggcacc acttccaaaa aatgcgcatt ttactaagtc gaacggcact
     1861 ggtggagggg tcgaaaaggt tggtacgccc ggtgcgacga aattagaaga tttagagcga
     1921 accctcaagg tttcgatggt cgatatgatt aagtctgtgg cctatgagct ggtagtgccc
     1981 ggcgtgggag agtctcgtcc tgtggtggta tttattcgtg gagatttgcc ggtatccgag
     2041 actaaattga aaaaatattt agatcgcggt gaactgcggc ccctctcggt tgataccgcg
     2101 aaaaaattcg gactgacgct gggttttatg gggccgtcta attcacccaa aacaggggta
     2161 atctatcttt tcgatcactc gttgtcgcag ccaggtaaat tctgggtgtc gggtgccaac
     2221 gaaacagact ttcactttaa aaattttcag gaaggtagag actttcaagt acacgaaagc
     2281 aaagacctcg cgcttgccaa aaaaggcgat cgttgcgacg aaaccaatgt ttacgaaagt
     2341 gctcgtggca ttgagatcgg acatattttt cagttgggga aaaaatatac cgaagcgatg
     2401 aaagtatcgt tgcacggctc tgacggcaaa ccaatggttc cgaccatggg ttgttacggg
     2461 attggagtgt cgcgactgat ggcgtccgct atcgagcaaa acaatgatga gcgcggaatg
     2521 atctggccta aggcgatagc tccgtaccaa gtgcacctca tttgtagcaa tatgaagaat
     2581 gacgcaattc gtcaggcagc agaagctttc tacggtgaat tgggtgagat ggggttcgaa
     2641 gtattgttcg atgatcgtga actgcagttt ggcgcgaaaa ttacggacgc cgaattgtgt
     2701 ggcatgccaa ttattgtggt tattggcaat gcgtttgctt ccagcggaca attcgaagtg
     2761 atcattcgtc agggattaat caaacaacag ctcggcaaag aagcgaccgt ctcgctcctc
     2821 aaatcgaccc ttttatgagg gagaggcagt ttctgcatta tatctacgat tagttgagtc
     2881 cgatttaaga gaattaattt gcctatcgga ctctgactcc gaacgagcgt accttttttg
     2941 cagccagctt tcgataccgc gtactatgac gaaggccgtc aatgagtaaa gtatgcccag
     3001 tataacatca ataatgtaat ggtgattcaa atagacggca ctaaagcaca tcaaaaggaa
     3061 aaatacgatg gcgggagtcc ggaatctttt aaccgacaac gccgcccacg cgactaacag
     3121 tgggtaactt acgtgcagtg acggaatggc accatacaca tcgacgctca taccgtacat
     3181 ggcatcaaaa aagtgggtac ccattaattg gtcgaaacga gcagctcctg ccgggctggc
     3241 caccacatct gtacgagcag gaccaaggcc ataggcagca acataccagg gcggtgcggc
     3301 ggggtatata aagtaggtcg caaaacccat tatgttaaca gtaaaaaagc accacccaaa
     3361 ggtacgaagg acggccagct gtcgggcaaa taatagtaga aagccggttc cgagatattc
     3421 aaaaacgaag gtgatataag caaatcctgc gaaaaaatcg agaatcttcc agttgttacg
     3481 ttcaaagtat tcgtttgggg ttaaaacagt tccatttgat tcaatgccga atagtttttt
     3541 ctctagaaaa tagggctctg aaacgtgcac gtttccttcg attccctgcc agtaataata
     3601 gcgcatcgaa tcgtaagcca ttcccgtcaa aatgaaaggg gtgaaatatt ttagaaacgt
     3661 acgagttctg gaattatagg aatccaacag taccaatgat gcgattatta catgttttga
     3721 ttctaggcca cctaagacaa atatcgtcaa aacataggcg agtcccaatg ctaaaggcca
     3781 ccatctacgc tttaaagcgc gttcaggaat gagccaaaga gcgatttctt gcgaaaggag
     3841 atttgcggta ctcatgattt cagtgttaaa accgcaacgt atgttgcacc tacgtttgtt
     3901 gcaagttgtt tttagtgttg ttttttttgg cttcggaccc ttagcgctcg ctactgaggg
     3961 ggcttccgcg tgttttgatt tttcaaaaaa ggtggccgat cccctgcgaa ccgcccccgc
     4021 ctggaagtcg gatcgtaggg aagatgtgag cggccgcggt aaagtcactc ggcatgagcc
     4081 gggaaaaccc aaaagcgggt atgtatgggg ccaggcgcgg ggagttgttc gaaaaccaat
     4141 acgcgagctc ctgaatcttc tgcttgatca caacaccaca aaaagtagta aaattcggga
     4201 aatcaatttt tcgaagtcgt ctgatgaaaa ctacttcgct cggcatgtaa ttacttttga
     4261 agtgacagca ctcattttct tttccgcaga atggaccgag gaatggggtt atgcgattac
     4321 gcgcggatca ggcatggctc cggaacgcgt ggtgatttct taccaaaaga ccgctggcac
     4381 cagccacatc gagcatttat gtggaagcat tgttttagaa atgatcgacg cgaacactac
     4441 tgacgtttat gcatacgaag aagtgaatgc aaatcagaga aacgacgaag acactgaaag
     4501 cggcttagtc ggtacgttat taactttgcg aaagtgaaaa aaatgatttt gactaaagga
     4561 ctccaagccg tctacgcggc gacaatcgac cgatcaaaat ttcgagaggt tgtttcgcgt
     4621 ctagtagtat ttgtactgcc acttttttac tggggtcggc agccatggtg gtggggtcta
     4681 ttattcgtag gtgccggtat attgctgaga gcgtgggctg ccggatattt gcacaaagat
     4741 gaggagcagg cagcggctgg gccatatctc ttggtgcgcc accctttgta tttggggtcc
     4801 tgcctgcttg cactcggttt gattattact ttgcatcatt ggttcgttac attagcgatt
     4861 ggcggagtga cggctcttac atacctacac actattcggc atgaagaaaa aaatctcctc
     4921 aagagattcg gggagtctta cgctcgatac tgcaatgaaa ccggtcctct ttggcctcgc
     4981 gtcggtgggc ttaaaaaatt ctttgctttt aaaagcaaga ggaagttttc gtttcgtcag
     5041 tatttaaaaa acaaagagta cgaatgtttg ttgggagtag tagttgttct cggagttctt
     5101 tatttgggtg ctggatcgaa atgagactga cggtcgtagt tcctaccttt aatgaagctg
     5161 acactattga aaactcgtta cgcgaaatcg aacgtgtctt gggtccagaa ttgtcgaagg
     5221 ataccgagtt attgattgcc gacgatggtt ccgacccaat gccagagttg gtagaacgtc
     5281 taaaaaaaga aatcggtttt gccagattag aattacttcg gaactcgccg cctgtcggaa
     5341 aggggctctc cttagctcgg gcatttgagc gagcccaggg gcgaacggtt ggttttcttg
     5401 atgcagatct ttcgacccct gccacataca tactcgaagc tctaaaagtc ctcgaagcga
     5461 atgaggccga tctttatatc ggatcacgaa gagcgatggg tgcaaatgta gtgcgcgagc
     5521 aattctggct gaaggacgtt ctcggtgatg cgcttcgagt tggcgtaaac gcagtcatat
     5581 ttagactagg ccgaaaatac agtgacactc aatgcggttt taaattcttt aagaaccaag
     5641 tcgcgaaggt attgtattgc aacctcgttg cgtatgatgg ggttacagat gtcgaaattt
     5701 tggtaagagc taatctacta ggatatcgtg tgtgtgaacg aggcgtagaa tggtgtgata
     5761 cccgagaaag taagcggtct cttaaacgaa tactaatcgg ggatcttaaa gccataactc
     5821 gaataatgtg gcattacgga gtattcgaaa aaaaacaaat caaagacctt cgtcgttttg
     5881 aaaaaaccct tatataataa cgatatgttt aatttaaaaa agtggtcccc tcaggaagta
     5941 gttctcgcta cagcgatcgt tcttatcgca gcgatctaca gctatttctt tatttatttg
     6001 ccgcagtatt ggaccttacg cgacgattat ggctttgcac tcaaatgggg ggctaatccc
     6061 gacaaagttg tcgactatgg ttgggcctac ctaaaaggca taatgactca cggtcgtttt
     6121 caaccgatgg acgtcttgaa ccgcgtaata cattacggga tcctgccaat tgatgccagg
     6181 tactttcgaa tttccttttt tataagcgta ttcatatcgg tcgtaagttt tgtaggcatt
     6241 ttaagggtat ttggttgcgc tcgtgatcaa atactattgg cggtcttgct gttagtctcc
     6301 aatcattctc ttaaagaaat gctcacgacc atgactgtgt cggaacgtat cggcgctatg
     6361 tattttatgg ttgcgttatg ggcctattcc gccaattgga aaaattctca atggtgggca
     6421 ttgctaattt ttccactatc cttcttcgga aaagaaagct tcgtagtatt ggccccagtt
     6481 tttgccgttg ttgccttttt tcaaaaaaat aagaaaagta atgcgggttt gatagtcgcg
     6541 cttcttttga tgattgaagc cctaattttc gtaatattta ttcgaaattt gccaaaatta
     6601 tataccgaag gactatcgta
//
LOCUS       DAOMXO010000032         6579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6158_length_6579_cov_3.978234, whole genome shotgun sequence.
ACCESSION   DAOMXO010000032 DAOMXO010000000
VERSION     DAOMXO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6158_length_6579_cov_3.978234"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLH57_01300"
     CDS             complement(<1..306)
                     /locus_tag="PLH57_01300"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRK01275.1"
                     /translation="MNRAIELRVLGEGRILSTHRLTGNQITLGNTSLAEVPLQFPTVS
                     RFHAEIVVVENQVEVRDLGSRNGTYLGANKLEPHRSTAWPSAASLRIGPYELRWQPEP
                     "
     gene            complement(303..1754)
                     /locus_tag="PLH57_01305"
     CDS             complement(303..1754)
                     /locus_tag="PLH57_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01276.1"
                     /translation="MRFTRLSFGAIGALVLNFTLFAPPIFGTDDTIEISDTLEADPDA
                     SPITMGFVPQILLRVGERYPLEQTGQASADSLRISPQSVASIKNTASGPVILAHAPGR
                     AQLLVEANDGAPAKIDVVVREKLHKSTAPGDATLVGAIRGAKLRSLGGQKILEGVIEQ
                     RESYRNLLLLLRTKPKITVLATTTTALRESLALQAKQLLEKNGLDRVDITHAGNRFFI
                     DGQVATPEQVDQAYELASSVIPNIENHLQIPMRITPSITVRVFLLELTRSAHEVLGLR
                     WPGLVAGAGVVSPSSIAFGPKWSADLLHLARRGEAKVLAEPLLALKSGSAAELAAGGE
                     IPLRVTGRYENKVVWKHYGLKLKVHIAGIAGNVIRAKIETSTSSLDEASAIDGIPGIR
                     RNSLSTEIDAVENKPILLTGLFHASQSKDIEKLPILGDIPLLGELFKSRRFQNRESEL
                     LVAFLPRFGVEHRKTPLDSERGLNFDTKWRILD"
     gene            2034..3215
                     /gene="metK"
                     /locus_tag="PLH57_01310"
     CDS             2034..3215
                     /gene="metK"
                     /locus_tag="PLH57_01310"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRK01277.1"
                     /translation="MSANHLQSRYLFTSESVTEGHPDKLCDKVSDAVLDAFLKQDPKA
                     RVAVETLSATGLIVVAGQVTASGHPIDVQDIVRGAIREIGYDSSDKGFDYKTCAVLTQ
                     LDKQSPDISQGVTEGEGLHKEQGAGDQGLMFGYAIDETPEHMPLSIDLSHKLALRLTE
                     TRKSGLLPFLRPDGKTQVTVEYEADRPKRIDAIVVSSQHAESATYEQIREAIVEEVIK
                     KTVPKDLLDAKTKYFINPTGRFVLGGPMGDTGLTGRKIIVDTYGGHGAHGGGAFSGKD
                     PTKVDRSACYMARYAAKNVVAAGLARRCLVQVAYAIGVAEPVSLMVHTFGTESCAREK
                     IETAVRETFNFKPAGIIKTLRLERPIYAKTAAYGHFGRRDPEFTWENTDQVDALRSLC
                     R"
     gene            3673..4779
                     /locus_tag="PLH57_01315"
     CDS             3673..4779
                     /locus_tag="PLH57_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRK01278.1"
                     /translation="MGDDIQKSGERIPLDHLSACGMIGRSPAIQKVFEDIQKVADSDS
                     TVLISGESGTGKELVARAIHMLSSRRNASLVPVNCGAIPGELLESELFGHVKGAFTGA
                     ISNRQGRFELAQGGSIFLDEIGDMPSLLQVKLLRVLQERQFEPVGGTKAIVADVRIIA
                     ATNRDLEEAVKERSFREDLFYRLNVIPIHIPTLRERKADVPVLIEHFARKFCIEKKRN
                     ICFDAPEVMDLLLRYDWPGNVRELENLVERLVVFCDGTVQLKDLPPKVFERVELAASR
                     PGVQGSFGFATHTAQMLGAGNLALETGGSVPRFMLPDDGVDLRDLINQFENDLLMQAL
                     SRTRGNKNKASELLKMNRTTLVEKLKKKNLQFEV"
     gene            complement(4799..5278)
                     /locus_tag="PLH57_01320"
     CDS             complement(4799..5278)
                     /locus_tag="PLH57_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01279.1"
                     /translation="MRIALAFLGLIFISLAAALVHAAELRGTAYPCRVQLIGSGRSTG
                     NGLGVFGAVRVEFWTMPDCNGGYCGTAYFMSNDAWLMESATGATYTLNKEELLSQYQA
                     LVTTANAGGRVWWAMETSTQVPFIYELRVSTEEGAAFCKNREYTESTLGTAERVMGK"
     gene            complement(5463..6410)
                     /locus_tag="PLH57_01325"
     CDS             complement(5463..6410)
                     /locus_tag="PLH57_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01280.1"
                     /translation="MSILRYLLFFTALSACPLVRTGEAIELINPDFSNCDVVLRASEP
                     GVDPRLDSAHQAAARREAVRAIIDSLVESTLTQKLNSFTSPVQKNIWTKIRGRSSLSH
                     EEFTKNIGAMGSYSPLTQEIFIDELYYRSTFEAPLLVHESQHLIDHFKNRLEDHPAFN
                     FAIDMAATFRSATSTWDYEQSAHGAEYDFVKAVEQQLANHPYLSRNDLLLLLAHEVPI
                     PEQIFKPYQALLPFMVIEQNLFLINIANPAIDIVYDHENSHEAMSFVRDHLGRVIRNS
                     PWGALFFESKSAFIASQIGPRPTGGRLRANIKRLSKVLK"
BASE COUNT         1639 a         1522 c         1736 g         1682 t
ORIGIN      
        1 gggttctggt tgccatcgta attcgtaggg accaatacgt agtgaagcag ccgatggcca
       61 agccgtggac cgatggggtt ccaatttgtt ggcccctaaa taagttccat tgcgcgatcc
      121 aaggtcccgt acctcaacct ggttttcgac cactactatt tctgcgtgaa aacgtgacac
      181 cgtgggaaac tgaagcggta cttctgcgag cgaagtattg cccaatgtta tttgattccc
      241 ggttaaacgg tgggtgctaa gaatccgacc ttcgcctagc acgcgaagtt caatggcccg
      301 gttcaatcga gtattctcca tttagtatcg aagttcaaac cccgttccga gtcgagcgga
      361 gttttgcggt gttcaacgcc aaaacgtgga aggaatgcga ccagtagttc gctttcgcga
      421 ttctggaatc tgcgtgattt aaaaagctcc cccaatagcg gaatgtcccc taaaattggg
      481 agcttttcga tatccttgct ctgagaggcg tgaaaaaggc ccgtgagcaa tatgggtttg
      541 ttttccaccg cgtcgatttc agtgcttagt gaatttcgtc gtatgcccgg aataccatcg
      601 atggccgagg cttcatcgag cgaactagtg gatgtttcaa tcttggcccg aatcacattt
      661 cccgcgattc cggcgatatg cactttgagt ttcagtccat aatgcttcca gacaacctta
      721 ttttcgtagc ggccggttac tcgcagcgga atctcgccac cagcggcgag ctctgctgcg
      781 gaaccgcttt taagcgcaag taaaggttca gctaatactt tggcctctcc ccggcgggcc
      841 aggtgcaaga ggtcagcgct ccatttcggt ccgaacgcga tcgaagaggg ggatacgacg
      901 cccgcgccgg ccacgaggcc gggccagcga aggcccagta cttcgtgagc gctccgggtt
      961 agttcaagca aaaacacccg tactgtaatt gacggagtga ttcgcatggg gatctggagg
     1021 tgattttcaa tgttggggat caccgaagaa gctagttcgt aggcttgatc gacttgctcg
     1081 ggggtggcca cttgaccatc gataaaaaaa cggtttccgg cgtgagtgat gtccacgcga
     1141 tcgagaccgt ttttttcaag tagttgtttg gcctgtagag ccaagctttc acggagcgcg
     1201 gtggtagtgg tcgcgaggac cgtgatttta ggtttggtcc gcaaaagtag cagaaggttg
     1261 cgatagcttt cccgttgttc aattacgccc tcgagtattt tttgaccccc caaactcctg
     1321 agttttgcgc ctcgaatggc tccgaccaac gtagcatcgc ctggagcggt agatttatgt
     1381 aacttttcgc gaaccaccac gtcgatcttg gcgggcgcgc cgtcgtttgc ttctactaga
     1441 agctgagccc ggcctggggc gtgggcaaga atcacagggc ccgaagcggt gttttttata
     1501 gaagcgacgc tttgaggtga aattcggagg gagtccgccg aggcctggcc ggtctgttcc
     1561 aaagggtatc gctctccgac tcgtagcaga atttggggga cgaaacccat ggttattgga
     1621 gaagcgtccg gatcggcttc tagggtgtcg ctgatttcga tagtgtcatc ggtcccgaat
     1681 atcggggggg cgaataatgt gaaattgagt actagggctc cgatcgcgcc aaaagacaat
     1741 ctagtaaaac gcatagtttt gacttcaaac ctccaggtgc gcctttagca aggcccgcgc
     1801 caacacctga ttgacaccaa acactcccct acagtatggt gacttttttt aaggttttaa
     1861 tgaaggagaa ccaaccccat ggcatcacgc acaggtagaa cgcgtttaca gcgaaagacc
     1921 cgaaaggcgc gaaagggaaa ggcacgaaag cgccaggcgc gcaacaaggg cacaacccca
     1981 aagttttcgg ttcatctcaa ctaactagaa gttcacatca aaaaggtaaa ttcatgagcg
     2041 caaatcatct tcaaagtcgt tatcttttca ccagtgaatc tgtcaccgaa ggccatccag
     2101 ataaactttg cgataaggtt tcagacgcag tgcttgatgc ctttttgaaa caagatccaa
     2161 aagcgcgagt cgcagtcgaa acgctttcgg cgaccggatt gatagtggtc gccggccaag
     2221 tgacggcttc tggccaccca attgatgttc aggatattgt acgtggcgca attcgtgaaa
     2281 ttggttacga ctcatcggac aaggggttcg actataaaac ttgcgctgtt ttaacacagc
     2341 tcgataaaca gtcgcccgac atttcgcaag gtgtaacaga gggcgagggg cttcataaag
     2401 agcaaggagc cggcgaccaa gggctaatgt ttggctacgc gattgatgaa actccagagc
     2461 acatgccact ttcgattgat ctttcccaca aacttgcgtt gcgtttaacc gagactcgca
     2521 aatccggttt gctcccattt ttgcgtcccg atggcaaaac tcaggtgacg gttgaatatg
     2581 aagcagatcg tccgaaacgc attgatgcga tcgtagtttc gagtcagcac gctgagtcgg
     2641 caacttacga gcagattcgc gaagcgatcg tcgaagaagt tattaaaaag acagttccca
     2701 aagatctact cgatgcgaaa acaaaatatt ttattaatcc taccggtcgt tttgttctcg
     2761 gtgggcctat gggcgacact ggtttgacgg gacgaaaaat tattgtcgac acttacggcg
     2821 gacacggcgc ccacggtggt ggagcgttta gtggtaaaga tccaacgaag gtagatcgct
     2881 ctgcttgtta tatggcgcgt tatgcagcca agaacgtggt tgccgctgga ttggcccgtc
     2941 gctgtttagt tcaagtggct tatgcgatcg gtgtggctga gccggtttca ttgatggtgc
     3001 acactttcgg tacagaatct tgtgcccggg aaaaaattga gaccgcagta cgtgagactt
     3061 ttaatttcaa gccggccggc atcattaaga cgcttcgttt ggagcgtccg atttatgcaa
     3121 aaacggcagc ctacggtcac tttggtcgtc gtgatcctga gtttacttgg gaaaacacag
     3181 atcaggttga tgcgttgcgc tcactctgtc gctaaaacgc gtgtacacga ttctattttt
     3241 ggttcgatat cttccctttt gggcgatacc cgccgcgctc atgctttttg agctcggcgt
     3301 gtatcattac aaccgtcgca atcgaatcgg cctattcgga agcttcggtc tcttcgctgt
     3361 cttggtcgtc gcgtcaatat tctggcttgt tttcgagggg tactggcgcg gtggccccgc
     3421 agcaaaaagg tttttagaat actacggctt ttaggcctgt tgtcggcatt aaaccagaaa
     3481 ctgaagtgat ctaagtaggt tagaaggttt tcctgttaaa ttattaggat tgtaggagtt
     3541 tttttcctct cgttcccctg acaataatca aaatttttga cgttggtaaa ttgacacgag
     3601 ttggtttcct gttagcatca aaagacaggg cggcaatcgc cctttgatct tctaggacgg
     3661 aggaccaagc gagtggggga tgacattcaa aaatcagggg aaagaatccc tttagaccat
     3721 ctttccgcat gcggaatgat cggtcgtagc ccagcgattc aaaaggtatt cgaagacatt
     3781 caaaaagtag ccgactcaga cagtacagtt cttatatctg gcgagtcggg cacgggaaaa
     3841 gagttggtcg cacgcgcgat acacatgctc tcatctaggc gcaatgcgtc gcttgtcccg
     3901 gttaactgcg gagcgattcc gggtgaattg cttgagagtg aactgtttgg ccacgtcaaa
     3961 ggcgctttta cgggcgccat ttccaatcgc cagggacgct ttgagttggc gcagggcggc
     4021 agcatctttt tggatgaaat cggagacatg ccatcgcttt tgcaagtgaa gttgctgcgt
     4081 gttttgcagg aacgccagtt cgagccagtg ggtggcacta aagcaatcgt cgcagatgtt
     4141 cggatcatag cagcgaccaa tcgtgatctg gaagaagcgg ttaaagagag aagctttcga
     4201 gaagatcttt tttatcgcct aaacgtgata ccgattcata ttcccacgtt acgtgaacgc
     4261 aaagcggacg taccggtttt gattgaacat tttgcccgta aattttgtat cgagaaaaaa
     4321 cgaaatattt gtttcgacgc tcccgaagtc atggatctgt tgttgcgcta cgattggccc
     4381 ggcaatgtgc gcgaattgga gaacctagtc gaacgccttg ttgttttttg tgatggaacc
     4441 gtgcagctga aagacctgcc ccctaaagta tttgaacgtg ttgaattggc cgccagccgc
     4501 cctggcgttc agggtagttt tggttttgcg acacatactg ctcaaatgct tggcgcagga
     4561 aatctcgcgc tcgaaaccgg cggtagtgtg ccgcgtttca tgttgcccga cgacggggta
     4621 gacttgcgcg acctcatcaa tcaattcgaa aacgatctgc tcatgcaagc actctctcgc
     4681 actcgcggta ataaaaacaa ggcaagcgaa cttctcaaaa tgaatcgcac cacccttgtg
     4741 gaaaaactaa aaaagaagaa tctgcagttc gaagtgtgag tcggtgacca agcctaggtc
     4801 atttacccat tacgcgttcg gcagttccta gagttgattc agtatattct cggtttttgc
     4861 aaaaagcggc accctcttcg gttgaaaccc gcaattcgta aataaaagga acctgagtcg
     4921 aagtttccat tgcccaccat actcgcccgc ctgcattggc ggtggtgact agagcttgat
     4981 actgagaaag gagttcctct ttattgagcg tgtaggtagc tcccgtcgca ctttccatta
     5041 accaagcgtc gttagacatg aagtacgcgg ttccacagta ccctccattg caatcaggca
     5101 tggtccaaaa ttccactcga acggcgccaa acacgccgag tccatttccg gtggaacgac
     5161 cggatccgat caattgtacg cgacaggggt aggccgtgcc tcggagctct gcggcgtgaa
     5221 ccaaagcggc cgccagtgaa ataaagatta gtcccaaaaa agcgagtgca atacgcatta
     5281 aaaaccccca aagttgtaat tttgcgatgt acggctcgaa gctaatctga cgagtagagt
     5341 tcgtaaatga attttagcgt gaactgccac gttcgaagtg cgcgtgtagc tactgctagg
     5401 ccaagatttt tagacgaaac atgaggtgaa ctcgccgttg gctcttcgga taaaatcgaa
     5461 atttatttca gaactttaga caggcgtttt atgttggctc taagcctgcc cccggtcggg
     5521 cgtggtccaa tttgacttgc gataaaagct gattttgatt cgaagaataa agcaccccac
     5581 ggactattgc gaatcacacg acctaagtgg tctcgaacaa agctcatggc ttcgtgggag
     5641 ttttcgtgat cgtaaactat atcaatcgct gggtttgcaa tatttatcag aaataagttc
     5701 tgctcgatca ccatgaaagg tagcagtgct tgatagggtt taaaaatctg ttcgggaatt
     5761 ggcacttcgt gggcaagtag aagtaacaga tcgttacgac tgagataggg gtggtttgca
     5821 agttgttgct caacggcttt tacgaaatcg tattccgcgc cgtgcgcaga ttgttcgtag
     5881 tcccaagtag aagtggcgct tcgaaaagta gcggccatgt cgattgcgaa attaaaggct
     5941 ggatggtcct ccaggcgatt tttgaaatgg tcgatcaagt gctggctttc atgtactaaa
     6001 agcggtgctt cgaacgtcga acgataataa agttcatcga tgaatatttc ctgagtcagg
     6061 ggcgagtaac ttcccatggc cccaatgttc ttcgtaaatt cctcatgaga aagagacgag
     6121 cgcccacgga ttttggtcca aatgttcttt tgaacggggc tggtaaagga atttaatttc
     6181 tgggtcagag tggactctac caaagagtca ataatggcgc gcaccgcttc gcgacgagcg
     6241 gctgcttgat gggcggagtc cagccgggga tctacaccgg gttcactcgc gcgaagaacc
     6301 acgtcacagt tagaaaaatc gggattgatt aactcaatag cctcaccggt tcgaaccagt
     6361 gggcatgcgg aaagcgcagt gaaaaagagc aaatatcgaa gaattgacac tgtgcgtttt
     6421 tactaaagaa gacaataaaa gtcaacatta ggtaatggtt agaaaaaatt aacgatcgag
     6481 ggggttaccg ctagtttgtt ttttcagctt tttcggcttt ttcgcgggaa gtttcgacgc
     6541 tcttttttat gaagtatttc aggaccttgt tgcgattgg
//
LOCUS       DAOMXO010000033         6557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6190_length_6557_cov_4.887112, whole genome shotgun sequence.
ACCESSION   DAOMXO010000033 DAOMXO010000000
VERSION     DAOMXO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6190_length_6557_cov_4.887112"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2131)
                     /locus_tag="PLH57_01330"
     CDS             complement(<1..2131)
                     /locus_tag="PLH57_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRK01281.1"
                     /translation="MNAAEQKSLVDVLRGRARETPDRLAFNFLLDGESLEAPVTYAEL
                     DARARAIGAVLQKKGLTGKPVVLLYAPGPEYIAGFFGCLYAGAIAVPAYPPNPSQIRL
                     TVKKLQGIMKDAGCAAVLATSDIVLRLKPLFLLSPSMWRLKWVPTDRVEDSAGDRWVE
                     TELTRDHLAFLQYTSGSTGEPKGVMLSHGNLLSNLESIRIGFGYTPDSRGMVWLPPYH
                     DMGLIGGILCPIFTGFPVVLMSPIDFLTKPLRWLQAISKYKATVSGGPNFAYALCVRK
                     ISAEDRASLDLSSWKVAFNGAEPIRNEVLESFSQTFKGQGFKKEAFFPCYGLAESALF
                     VTGIPPGRGAVSVVVDSAELEKNKVVLRPDQSTPGDVSSKRLVGCGMSAVAQKMLIVN
                     PDTCEICTDGSIGEVWVSSASVAKGYWKQEERSEKTFRARVKNQHGLGAFLRTGDLGF
                     ITASGELYVTGRLKDLIIIRGRNHYPQDIERTVEQATHSVRPGCSAAFAIDVGGDEKL
                     VVVSEVERRFSPTAKRSIVGEGIKNEPERLKELDSEAVFQAIRKAVGHNHEIQIHEIV
                     LVRAGSVPKTSSGKIRRSTTKQAYLSKELHTVATDLFGTGTPKRPLNAVNDSNKNSEA
                     ESSNALPRVLHENQPGIEKELREKLAAAMNAPVDSLDVTQPMSSIGLDSLHLVEIGTY
                     IQGRYGIDLSFEKFFGDTSIAALANELA"
     gene            complement(2133..4466)
                     /locus_tag="PLH57_01335"
     CDS             complement(2133..4466)
                     /locus_tag="PLH57_01335"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRK01282.1"
                     /translation="MMMGVFIRWLIAHARLVLVLVGLVTLGLAAGIPRLRLESNIENN
                     LPSTHSYIRAFETIKDKFGISDVIIVAFRKKEGSVLERDFLLMLEKITSELGTIPGAS
                     SANILSLTARKVKDIRGVADGLDVKPLSDFTGSVRELDEAIKRNKVYERVLITDDRRA
                     SAIVMDYKPEGDPHYQQNEKHIREIVERHLLPNIEYGMNGAPIIMSALEKYSEKIALY
                     FLIAIVVIGLVHFEAFRSFQGLVLPLVTALLSVVWSTGALCWLGFNTLDPITAPTPIL
                     ILAIAAGHAVQVLKRFYEEFDVVKDNKLAVQRSLEKVGPVMVLAGSIATISFLTLLLF
                     DIPSIRNFGLYSALGVMAALAIELTFIPALRSLMPAPKAIRHKDHALLDRVMTWLSDR
                     LVLKKNFKLVLTTTLVLCIFCLAFTFRIKVENSPKIMLPEDERVLVEDEWIGRTVAGV
                     ATIWFLLEGKEDDLKRPDVLIAADQFMQFLESDPDVSKTMGLPYFVKKMNQGMNEGRN
                     EKFHIPMNQDLIAQYLFLYSMSGDPGDFDSYVTPQYDKAVITAFLKNSSTAYTEKLIA
                     KANEQAAKLFPADIKFSVSGTQANIAAINRVIVNGKLGNILQIVFSVFVLTGLIFRSP
                     VAGAFAIAPIVIAVIVNFGIMGAAGIRLDVATAIVSALAIGIGADYAVYFISRLREEL
                     LRSGDYSEAGWIRSTRHTLVTTGKGIFFVSSAVALGYLTLCLSPFKPHFFMGLLVGTA
                     MVVSSAAAVVILPIFALLFKPRFLIRTERSVEHKEAA"
     gene            complement(4561..5403)
                     /locus_tag="PLH57_01340"
     CDS             complement(4561..5403)
                     /locus_tag="PLH57_01340"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRK01283.1"
                     /translation="MTSMYRILWSVLLSFGCLVSYRASAVENTSSVAMSGTQIMEHNF
                     KATRLKQSRVTATMELISSKGEKRVRQQRGVSKLKPNGLDSRGLTVFDLPNDIKNTAV
                     LIDEDQADGDKVWIYLPALKKNRRLPGSGKTENFVGSDFTYGDIIVPKVAEYKHTLSG
                     QEPCPDDSKIKCYVVDSVPASSDILETRGVSAKRQWVRADNYMVVRYETKDRAGRPWK
                     SFLGKNPKLVDPKEGKYLLFDVEITDIQSKHRTRLILNKVDVVSPVSDDLLTTMQLER
                     GLPQ"
     gene            complement(5400..6125)
                     /locus_tag="PLH57_01345"
     CDS             complement(5400..6125)
                     /locus_tag="PLH57_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRK01284.1"
                     /translation="MLKNCRARTKPEIRMVKLKDHLKFVDKYYSTLSVDERSRADRFT
                     IKNVRERFIVSRALLRTLIAKKIGIKTNAIVFAYGPHGKPQLDAVMTGISLKEPFHFN
                     VSHSKSMVLFCFGLLPVGADIEFVRKLNNPDRIAQRIISKRKLKIFRSLPLKMRHEVL
                     LQIWTRKESIVKALGCGLTIPVKNFEVWTGSKADPLSFARRVEIPDERFGDLIIESLA
                     LKLPKTYVGAVSFICGNKQKDNP"
     gene            6360..>6557
                     /locus_tag="PLH57_01350"
     CDS             6360..>6557
                     /locus_tag="PLH57_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01285.1"
                     /translation="MQSKKFLSVFAFLVMPFVGHASVHEVSCLDLIFSSTHDAKRVDQ
                     NDVQTNIEKILERRLGVPNSHG"
BASE COUNT         1770 a         1600 c         1412 g         1775 t
ORIGIN      
        1 aagcaagctc gttggctaag gctgcaatac tggtgtcgcc aaagaacttt tcaaacgaca
       61 agtcgattcc gtaacgacct tgaatgtagg ttccaatttc caccaaatgc agtgagtcta
      121 acccaatcga agacatcggc tgagttacat cgagagaatc gaccggagcg ttcattgcag
      181 ccgccagttt ttcgcgcagt tccttttcga tacctggctg attttcatga agaactcgcg
      241 gcagggcgtt cgaactttcg gcctcactat ttttattcga atcattaaca gcgttcagag
      301 gtctcttagg tgttcctgtt ccaaacaggt cagtcgccac cgtgtgaagc tccttggata
      361 agtacgcttg cttggtggtg cttcgtcgaa tttttccgct cgaagttttt ggcacactac
      421 ctgcgcgcac caatacaatt tcatgaattt gtatctcgtg attgtggcct accgctttgc
      481 ggatggcctg aaacactgct tcggaatcaa gttcttttaa tcgttctggc tcgtttttaa
      541 ttccctctcc cacaatagaa cgcttagccg taggggagaa gcggcgctca acttcagaaa
      601 cgaccactaa tttttcgtcg ccacctacat cgattgcaaa cgcggcactg caaccaggcc
      661 gaactgagtg ggtcgcttgc tctacggtcc gttcgatgtc ttggggatag tgattacgtc
      721 cgcgaataat tataagatct tttaatcgtc cagtgacgta tagctcgccg cttgccgtga
      781 taaaacccaa atcaccggtt ctaagaaacg cacctaaacc gtgttgattt tttactcggg
      841 cccgaaaagt tttctccgag cgctcctctt gtttccagta gcctttcgct acgctagcgc
      901 tagaaaccca cacttcgcct attgatccat cagtgcatat ttcacaagtg tcgggattca
      961 cgatcagcat tttctgcgcc accgcggaca ttccacagcc gaccaaccgc ttacttgaaa
     1021 cgtcgcccgg agtactttga tcaggccgta acacaacttt atttttttct aactcggccg
     1081 agtccaccac aaccgaaaca gctccgcgcc caggtggaat acctgttacg aaaagtgccg
     1141 attcagctaa gccataacag ggaaaaaaag cttctttttt aaaaccctga cctttaaacg
     1201 tctgggaaaa cgactcgagc acttcgttac gaatgggctc ggcaccatta aatgccactt
     1261 tccatgaact gaggtccagg ctcgcccgat cttccgctga aatttttcgt acacagagcg
     1321 cataggcaaa attaggaccg ccactaaccg ttgctttgta ttttgagatt gcttgcaacc
     1381 aacgcagcgg ctttgttaaa aaatcaatcg gtgacattaa aaccactggg aatccagtaa
     1441 atatcggaca aagaataccg ccaatcaaac ccatatcatg gtacggcggc aaccacacca
     1501 ttcctcgact atccggagta taaccaaagc caattctgat cgactctaga ttcgaaagca
     1561 aatttccgtg cgacaacata acgcctttcg gctcgccggt ggatccggag gtatattgaa
     1621 gaaacgctag gtgatcgcgc gtaagttcgg tttcgaccca gcgatctcca gcagagtctt
     1681 caacgcgatc ggtgggaacc cacttaaggc gccacatcga aggcgatagc aagaaaaggg
     1741 gttttaaacg aagcacaatg tccgaggttg caagaactgc ggcgcaacct gcgtccttca
     1801 taattccttg aagttttttt acagtaaggc gaatttgcga aggattgggt ggataggccg
     1861 gcactgctat cgcacctgca taaagacaac caaaaaatcc cgcgatgtat tcgggccccg
     1921 gtgcataaag taaaacaact ggttttccag taagaccttt cttttgcaaa accgcaccta
     1981 tagcacgcgc tcgagcatcg agttccgcat acgttacggg cgcctcaaga gactccccgt
     2041 ctagtaaaaa attaaatgct aatcggtctg gagtttcgcg ggcccggcct cgcaaaacat
     2101 cgactaagct tttctgttcg gcggcattca tcttaagcgg cctccttatg ttcaactgat
     2161 ctctcggtac gaattaaaaa tctgggttta aataacaaag caaaaatcgg caaaattact
     2221 accgccgctg cgctcgaaac aaccatggcg gttccaacta acaggcccat aaagaaatgt
     2281 ggcttaaatg gagataagca aagagtaaga taaccaagtg cgaccgcaga ggacacgaag
     2341 aagattcctt taccagtggt caccagcgtg tggcgcgtgg agcgaatcca accggcctca
     2401 ctatagtcgc ccgatcgcaa cagctcttct cttaaccttg aaataaaata aaccgcgtaa
     2461 tcggcaccga taccaatggc caaggcagag acaatggcgg ttgcgacatc taaacgtatg
     2521 ccagcggcgc ccatgatgcc aaagttaacg attacggcga tcacaatggg ggcaatcgca
     2581 aaggcccccg caacggggga tctaaaaatg agtccagtta aaacaaatac agaaaaaacg
     2641 atttgtaata tattaccaag ttttccattt acgatcactc gattaattgc agcgatattg
     2701 gcctgggttc cgctaacgct aaatttgata tctgcgggaa aaagttttgc ggcctgctca
     2761 ttggcttttg caattaattt ttcagtatac gcggtggaac tatttttgag gaatgcagtt
     2821 ataacagcct tgtcatattg aggagtaacg taagaatcga aatctccagg atcgccgctc
     2881 atactgtaca aaaagaggta ttgggcaatt aaatcttggt tcattggtat atgaaatttt
     2941 tcatttcggc cttcgttcat gccttgattc atttttttta cgaagtacgg cagtcccatt
     3001 gttttactta catccggatc agactctaga aattgcatga actgatctgc agcaatcaaa
     3061 acatctggac gtttcaaatc atcttctttg ccttcgagga gaaaccaaat cgtagcaaca
     3121 ccggcgacgg ttcgcccgat ccattcatct tcaaccaata cgcgctcgtc ctcgggcagc
     3181 ataattttag gagagttttc cactttgatt ctaaaggtaa atgctaaaca gaaaatgcaa
     3241 agcaccaggg tggtagttaa aactaattta aaatttttct ttagtaccag gcggtcactt
     3301 aaccaggtca ttacgcgatc gaggagagca tggtctttgt ggcgaatcgc tttgggcgcc
     3361 ggcattagac ttcggagcgc gggaataaaa gtcaattcaa tcgcaagggc tgccattacg
     3421 cccaatgcac tatagagacc gaaattacga attgaaggaa tatcaaacaa taaaagtgtc
     3481 aaaaaactaa tcgtagcgat tgatcccgcc agcaccatga cgggacctac cttttccaac
     3541 gaacgctgca ccgcgagttt attgtctttc actacgtcaa actcttcgta aaaccttttt
     3601 aaaacttgga cggcgtgacc cgcggctatc gctagaatca aaattggtgt cggtgcggtg
     3661 atcgggtcaa gtgtattgaa ccccaaccaa caaagtgcac ctgtggacca gacgactgaa
     3721 agcagtgcag ttacaagcgg caacactaac ccttgaaaac tccgaaatgc ttcgaagtgg
     3781 acaagcccaa taacaacgat ggctatcaaa aaatagagcg caattttttc actatatttt
     3841 tcgagcgccg acattattat tggcgcgcca ttcatgccgt actctatatt cggcagtagg
     3901 tgtcgttcga caatttcacg aatgtgtttt tcgttctgtt gatagtgcgg atcgccttcg
     3961 ggtttgtaat ccataacgat tgcgctcgcg cgccgatcgt cggtaataag aactctctca
     4021 taaaccttat tgcgtttaat cgcttcgtct agttcccgta ctgaacccgt aaaatcactt
     4081 aacggtttta cgtccaaccc gtcggccact ccccgaatgt cttttacctt acgggccgtt
     4141 agcgaaagta tgttcgcact ggaggctcca ggaatagttc cgagttcact ggtaattttc
     4201 tccaacatta ataagaaatc gcgttcgagt acgctgcctt ctttttttcg aaaagcgaca
     4261 ataattacgt cgctaattcc gaatttgtct ttgattgttt caaacgctct tatgtacgag
     4321 tgggtcgagg gcaaattgtt ttctatattg ctctcaagtc gaaggcgcgg aattccagcg
     4381 gctagtccca aggtaactag acctaccaac actaatacga gacgtgcgtg ggctatgagc
     4441 caccgtataa acacacccat catcatatct tttcggtcgg atattgattt ttcttgagag
     4501 gcgaatgaaa ttcgatagga atttcaataa tttacctaga cacaacggtc taaactgagc
     4561 ttactgaggc agccctcgct cgagctgcat tgtcgtcagc aaatcatcgg aaactggcga
     4621 caccacgtcg actttattaa gaattaatcg tgttcgatgc ttggattgta tgtccgttat
     4681 ttcaacatcg aacaagagat acttcccttc tttcgggtcc accaatttgg gattttttcc
     4741 caaaaaagat ttccacggtc ggccggctcg gtctttggtt tcataacgaa cgaccatgta
     4801 gttgtcggcg cgaacccatt gccgcttggc acttactccc cgggtctcga ggatatcgct
     4861 actcgcggga actgaatcca ccacgtagca tttaattttg gaatcgtcgg gacacggctc
     4921 ctggccagat aacgtatgtt tgtattcggc aactttcgga acaatgatat cgccgtaggt
     4981 gaagtcgctt cccacaaaat tttccgtttt gccgcttcca ggaagcctcc gattcttctt
     5041 taatgccggt agatatatcc aaactttgtc gccatcggcc tggtcttcgt caattaaaac
     5101 cgcagtattt ttaatatcgt tgggtaaatc aaatacggtt agcccgcggc tatcaaggcc
     5161 gttgggtttt aactttgaaa cgcctcgctg ttggcgcacg cgtttttcgc ccttactcga
     5221 aattagctcc atggttgcag tcacacgaga ttgctttaga cgagtggcct taaagttgtg
     5281 ttccattatt tgggtcccac tcattgccac tgaggaagta ttttctaccg cactcgctcg
     5341 ataggaaacg agacatccaa agctaagcag aactgaccaa agaattctgt acattgacgt
     5401 catgggttat ccttttgttt gttgccacaa ataaaactga ctgcgccaac ataggttttg
     5461 ggtaatttca gcgctaaact ttcaataatt aaatcgccaa aacgctcatc aggaatttct
     5521 acacgacgag caaatgaaag gggatcagcc ttcgagccag tccatacttc gaaatttttt
     5581 actggtatcg taaggccaca gccgagcgct ttaacgatag attcttttcg agtccaaatt
     5641 tgaagcaaaa cctcgtggcg cattttcaaa ggtagcgatc gaaaaatttt taattttctt
     5701 ttcgaaataa tgcgttgggc gatacggtcc gggttattta attttcggac aaattcgata
     5761 tcagctccaa ccggcagcag gccgaaacaa aataacacca tgcttttcga atgagacaca
     5821 ttaaagtgaa acggttcttt gagactgatt ccggtcatta ccgcatctag ctggggtttt
     5881 ccgtggggtc cgtatgcaaa aacaatcgca ttcgttttaa taccgatttt ttttgcaatt
     5941 aaagttctca ataaggctcg agacacaata aaccgctctc gcacgttctt gatcgtaaaa
     6001 cgatcagccc gacttctttc gtctaccgat aaagtggaat aatatttgtc tacgaacttc
     6061 agatgatctt tgagtttcac catgcggatt tctggtttgg tgcgtgcgcg acaatttttc
     6121 aacatgactc cattttaggc tcgatcaatt caaaggggaa tccaatctta atgacgttgg
     6181 ttcattgtga tgggttgcgt caatgaaaaa tttacccaca accttagtct atctgctttt
     6241 gcctctgctt cttactcgcg gaacagagcc cctctcgagc catcgacgcc ttgcgcgaaa
     6301 gaagacaaat ccgctgattt tagttttacg ctaaggaacg accgtgctac tcgggcgcaa
     6361 tgcaatcaaa aaaatttctg tcggttttcg cgtttttggt tatgccattt gtcggccatg
     6421 cttctgtcca cgaagtttct tgtcttgacc taatttttag ctccacccac gacgccaaac
     6481 gtgtcgacca aaacgatgtt caaactaata tagaaaaaat tctcgaacgc cgcttgggag
     6541 ttccaaactc ccacggt
//
LOCUS       DAOMXO010000034         6528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6224_length_6528_cov_4.707400, whole genome shotgun sequence.
ACCESSION   DAOMXO010000034 DAOMXO010000000
VERSION     DAOMXO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6224_length_6528_cov_4.707400"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLH57_01355"
     CDS             complement(<1..897)
                     /locus_tag="PLH57_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRK01286.1"
                     /translation="MILRYENLNYEQISGLNRGQTIVFIPCGPLEQHGPHLPLGVDAF
                     SAQYFAEQSAERMKTSPKYSQWSFILYPTIYLGADVFSHLGSLEIRPRTLRAVLSDVV
                     GTLAKNGFKNIVLIGAHGGPRHMVVLEEVAARARWRWRAKVICASSKIITDVLRGRFA
                     ERIEAHMKSQGDAMTDQERSAVKKDFHGGFFETSLMLLARPELVNQSYKTLSPAVLEN
                     VWKMRKHSLKTISPGLGYLGFPSLARPAFAKSAVAVLLDETIPWVERYFAGEKVEKKF
                     RSFLYYVPFLRTDFSLILVMLVY"
     gene            complement(910..1623)
                     /locus_tag="PLH57_01360"
     CDS             complement(910..1623)
                     /locus_tag="PLH57_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRK01287.1"
                     /translation="MSVSERLAEVRTRIEHACVRAGRKVEDIKLLAVSKFQTAGLIEE
                     AYQAGLRDFAENYVQSLEDRRSTLAHLSDARWHLIGHLQSNKVKSALQIANVFHALDS
                     EKLFDMISKRLKETPTWAPWPVFIEVNIDAEPQKAGISPEMAFSLVERVLGDRHLSLL
                     GFMCVPRADGSLGQLRESFVRMRLLRDKVQEDLKNKGINQPLLLNMGMSGDFEVAIEE
                     GADWVRLGTILFGERAPRT"
     gene            complement(1598..2221)
                     /locus_tag="PLH57_01365"
     CDS             complement(1598..2221)
                     /locus_tag="PLH57_01365"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRK01288.1"
                     /translation="MQLILASGSPRRQQMLRDLSLDFEVDIPRIIEKRLRGEPADIYV
                     KRLALDKARTVSEKRGLFGRSSLDWCVLAADTVVIQDRQVFEKPKNHADAVRMLKKLS
                     GRTHDVLTGYCWLGAFRGKCREVLASVRTKVTFAPRNSEFWEWYVSTGEPMDKAGAYA
                     AQGIGVSFIEKVSGSYANVVGLPLSQAIDTFEKNFGASVHECFRKAR"
     gene            complement(2226..3227)
                     /locus_tag="PLH57_01370"
     CDS             complement(2226..3227)
                     /locus_tag="PLH57_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01289.1"
                     /translation="MMRIILLSEKPEDRAFFEPVARLANLPFVNAGTAESLCASLLQD
                     PEALLVVDVSTEQIYKKFETVVAEKVGLYSDTVNVNRMFFVAGEDLQTLNYVAESDLF
                     GHLIMRPGSREVSAAPDQRTIAEDQARTSRLFSVAASDRGFGVERYFGPNVKIQEIMV
                     TKASQKKTIVESLKMKLLEVGFHSRAATSIATAADEIIMNAIFDAPTDDTGRVMYAQT
                     ARSTEMELSERARVSFKIMFDGTVLGLSVLDQYGSLDKKKLLGHLAKSYQNDQFKVKA
                     SVAGAGLGIANVFNTCGGMVFCCEMGVKTEVMLFYRKAKSYKEFKDQFRFLSTFMYL"
     gene            complement(3224..3691)
                     /locus_tag="PLH57_01375"
     CDS             complement(3224..3691)
                     /locus_tag="PLH57_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01290.1"
                     /translation="MIQVHKEVKGDILAIKIVGSIEENVDFAGIIGNITVPNVYVYLK
                     EVPRMNSVGVKSWVKYFQSIAAKGVNLKFLECSTAIVEQINLISNFTCGAKIESIYVP
                     FCCTSCGTELLGLFKSDDIKKINLAIPDLKCSKCGGKATFDDIPEEYFGFLMR"
     gene            3756..4649
                     /locus_tag="PLH57_01380"
     CDS             3756..4649
                     /locus_tag="PLH57_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01291.1"
                     /translation="MIKLDRTRQKLTTLAVLLHVVTFGFSAHAALVATSPSKTADFDP
                     SSLSKEEQSDYLRFPNLIRFARSISTDTLIATPPGNQHLIAAKEVFQPSQTVWNDFFA
                     AAVADKATNLRAFAIIAREVKRRGITLYGPGKDFERAVSDNKIDLGLSLPATKIGLGI
                     WAPNPANTDPEYQLSLSVIYSERYIHQFPDEVLPANLKIGFGDQVEYEIDGQKQSGFI
                     LTADVFNGPNGIGFKNVKGVGGQKRGLLGFFQKVFFFLPDAVHSMTINEKENTMITEA
                     LIDTVVKDFETREVYRIRKQL"
     gene            4722..5543
                     /locus_tag="PLH57_01385"
     CDS             4722..5543
                     /locus_tag="PLH57_01385"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRK01292.1"
                     /translation="MQSKQALLYTNNRIFNNAHKHKIAVDILERYTGSEASMFRKQVI
                     LTNFDYYVETFKKMSGTVATKGSAMNAAHNARLDVSIVDFSIGSPNAALIIEVLSVIE
                     PNAVLFLGMCGGLHRSLKVGDFILPMAAIRDEGASRHFMPSQVPALPTFKVQKFISQM
                     IVEKGLDYRTGVVHTTDYRFWEFNQRFKETLYEERALAIEMECATLFVAGFASKVPIG
                     ALLLVSDLPLRRGGIKTKKSAKAVFRKFTDVHLDLGIKSMSQIAGRGEHIRHYKW"
     gene            5557..>6528
                     /locus_tag="PLH57_01390"
     CDS             5557..>6528
                     /locus_tag="PLH57_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014245756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRK01293.1"
                     /translation="MFKKMFDWLSNDLAIDLGTANTLVFAKERGIIINEPSVVAVHKN
                     ARGQTKVLAVGKEAKEMLGRTPGSIVAIRPMKDGVIADFEVTQSMLKYFINKSNDRHS
                     FLRPRIIICIPFGITQVEKRAVKESAQSAGAREVYLIEEPMAAAIGAGLPITEPSGNM
                     IVDIGGGTTEVAVISLGGIVYSKSVRVAGDKFDEAIVNYIKRKYSLLIGERTAEQIKL
                     AIGNAYPFEEEKTYEVKGRDLIAGAPKTIEVNSEEIRDALADPVSAVIDAIKTALERT
                     PPELAADIVDNGIILAGGGALLANLDILIKEKTGLPVALAEDPLTCVV"
BASE COUNT         1799 a         1589 c         1457 g         1683 t
ORIGIN      
        1 gtatacgagc atcacaagaa taagtgaaaa atcggttctt aaaaaaggaa cataatagag
       61 aaaagaccga aatttctttt ccactttctc tccggcaaaa taacgttcta cccagggaat
      121 cgtttcatct aacaacactg caacggcact ttttgcaaac gcaggcctcg ctaagctcgg
      181 aaaccccaga taccccaagc caggagaaat cgtcttcaaa ctgtgttttc gcattttcca
      241 tacattttca agcacagcgg gcgaaagcgt tttgtaactt tgattcacaa gttcgggccg
      301 cgcaagaagc atgagtgagg tttcgaaaaa tccgccgtga aaatcttttt ttaccgcgga
      361 tcgctcctgg tcggtcatcg catccccttg ggatttcata tgagcctcga tacgttcagc
      421 aaaacgaccg cgcaatacgt cggttataat tttcgatgac gcacaaatca ctttggcccg
      481 ccaccgccac cgcgctcggg cagctacttc ttcgagtact accatgtgac gaggcccacc
      541 atgcgcgcca atcaaaacta tatttttgaa cccgttttta gccaaagttc ccacgacgtc
      601 gcttaggact gcgcgtaagg ttcgcggtcg aatttcaagc gatcctaaat gagaaaatac
      661 gtcggctccc aaataaatgg tgggataaag tataaaactc cactgcgaat acttgggcga
      721 agttttcatg cgttccgcag attgttctgc aaaatattgg gcagaaaagg catcaacacc
      781 taacggcaga tgcggtccgt gttgctccaa aggaccacat ggaatgaaaa caatggtctg
      841 ccctcgatta aggccactaa tttgttcgta attaagattt tcgtaacgaa ggatcattgc
      901 tagatcattc taagtccgag gcgcgcgttc tccaaacaga attgttccta atcgcaccca
      961 gtcagcgcct tcttcgatgg cgacctcaaa atcaccactc atgcccatgt ttaaaagcag
     1021 tggttggtta atgcctttat tctttaggtc ttcctgcact ttgtctcgca acaatctcat
     1081 tcgcacgaat gattcccgaa gctggcccag acttccgtct gctcggggaa cgcacatgaa
     1141 ccccaacaac gaaaggtggc ggtcgcctaa aactcgctca actaaactaa aggccatctc
     1201 gggggaaatt cccgctttct ggggctccgc atcgatattc acttctataa aaacaggcca
     1261 aggtgcccag gtgggggtct cctttaatct cttcgaaatc atatcgaata atttttcaga
     1321 atcgagcgca tgaaaaacat tcgcaatctg caacgctgac ttcactttat tggactgcaa
     1381 atggccaatt aagtgccagc gagcatcgct taagtgggct agagtagagc gtctgtcttc
     1441 taaagactgg acataatttt cagcgaaatc gcgaagacct gcttgatatg cttcttcgat
     1501 taatccggcg gtctgaaact tgctcaccgc cagcaatttg atgtcttcga cttttcggcc
     1561 tgcccgaaca caagcgtgtt caattcgagt acgtacctca gcgagccttt cggaaacact
     1621 catgaaccga tgctccaaaa ttcttttcaa atgtgtcaat agcctgagag aggggtaatc
     1681 ccacgacgtt ggcataagag ccgctcacct tttcgataaa cgacactccg atcccctgtg
     1741 ccgcgtaggc ccctgccttg tccattggtt caccagtgga aacgtaccat tcccaaaact
     1801 cgctatttcg aggcgcgaag gtcacctttg ttcgaactga ggcaagcact tcacggcact
     1861 ttcctcgaaa ggctccaagc caacaatacc cggtcaatac gtcatgagtt cgacccgata
     1921 gtttctttaa cattcgcact gcatcagcgt gattcttggg cttctcgaaa acttggcgat
     1981 cttgaataac cacggtgtcg gcggcgagga cacaccaatc tagagaacta cgtccgaaca
     2041 aacctcgttt ttcagaaacg gttcgggctt tatcgagtgc taagcgtttt acgtaaatat
     2101 cagcaggttc gccacgaagg cgtttttcaa taattcgcgg aatatcgact tcaaaatcga
     2161 gcgataaatc acgtaacatt tgctggcggc gcggggagcc agaggccagg atcaattgca
     2221 tgggctcaca aatacatgaa cgtcgataaa aagcgaaatt gatctttgaa ttctttgtaa
     2281 gattttgcct tgcgataaaa cagcataact tcggtcttga cgcccatttc acagcagaac
     2341 accatgccgc cgcaggtatt aaatacattg gcaataccta accccgcacc cgcgacagag
     2401 gctttaacct tgaattgatc gttctgataa gatttggcca aatgccctaa tagtttcttt
     2461 ttatcgagtg aaccatattg atcgagcacg gacagtccga gaaccgtgcc gtcaaacatg
     2521 atcttaaagc taactctcgc gcgttcactt aattccattt cagtgcttcg ggcggtttgc
     2581 gcatacatga ctcgaccagt atcgtctgtc ggggcatcaa aaatcgcgtt cataataatt
     2641 tcatcggcag cagttgcaat cgaggtcgcg gctcgtgagt gaaaccctac ttcgagtagt
     2701 ttcatcttga gcgactcaac tatcgttttt ttttgggacg ccttcgtgac cattatttct
     2761 tgtattttta catttggtcc gaagtagcgt tctaccccga aaccccggtc actagcggcc
     2821 acggagaaca agcgagacgt acgcgcctgg tcctcagcga tcgtacgctg gtccggcgcg
     2881 gctgaaactt cgcgcgagcc gggacgcatg attaaatgtc caaaaagatc gctttccgcc
     2941 acgtaattga gcgtctgcaa atcttcgccg gctacaaaaa acatacgatt tacattaacc
     3001 gtatcgctgt agagcccaac tttctcggcg accactgttt caaatttttt ataaatttgt
     3061 tctgtagaaa catcgacaac aagtaaagct tcggggtctt ggagcaacga tgcacaaagg
     3121 gactcggcgg tccctgcgtt cacaaaagga agatttgcca gtcgcgcgac cggctcaaaa
     3181 aaagcacgat cttcaggttt ttccgaaagc aaaatgattc tcatcatcgc atcaagaaac
     3241 caaaatattc ttctggtata tcatcaaagg tggctttgcc tccgcacttt gaacacttaa
     3301 gatcgggaat ggcgagattg atctttttta tatcgtccga cttaaagagc cccaaaagtt
     3361 cggtgccgca actggtacag caaaacggaa catatataga ttcaattttc gcaccacagg
     3421 taaagttcga gatcagattg atttgttcca cgatggccgt cgaacattcc aggaacttta
     3481 aattaacgcc ttttgctgcg atggattgaa agtattttac ccacgatttt acccccaccg
     3541 aattcatgcg gggtacttct ttgaggtaaa catagacgtt tggcaccgta atgttgccaa
     3601 tgattccagc gaaatcgacg ttttcctcaa tgctaccgac aatcttgata gctagaatat
     3661 cgcccttaac ttctttgtgt acctgaatca attcgcacct cttgagtaaa agaatctctc
     3721 tctatatagt ttgtcctaac tcaaagggga aatcgatgat taaactagac cgaaccaggc
     3781 agaaattgac gaccttggca gttttgctgc atgtagttac tttcgggttt tcagcgcatg
     3841 ccgcgcttgt tgcgacttcc ccatctaaaa cagccgactt cgacccgagt tccctttcca
     3901 aagaagaaca gtcggattat ttgcgatttc caaatctcat tcggtttgcc cgctcgattt
     3961 cgaccgacac tttgattgca actcctccgg gaaaccagca cttgatagcc gctaaagagg
     4021 tgtttcaacc atctcagacg gtttggaatg atttttttgc tgcggcggtg gctgataaag
     4081 ccactaacct gcgcgctttc gcgattatag ccagggaggt taaacgtcgc ggaataactc
     4141 tttacggtcc aggtaaagac ttcgaacggg ccgtttctga caataaaatc gatttgggtc
     4201 taagccttcc ggccacaaag attgggctcg gaatttgggc gcccaacccc gcaaacactg
     4261 acccagagta tcaactgagc ctttccgtta tctactcgga acgttacatt caccagttcc
     4321 cagacgaagt tttgccggca aatttgaaaa tcggatttgg ggaccaagtc gaatatgaaa
     4381 tcgatggcca aaagcaaagt ggcttcatac tgactgcaga cgtatttaat ggacccaacg
     4441 gtatcgggtt taagaacgta aagggtgtcg gcggccaaaa gcgaggtctt ttgggtttct
     4501 tccaaaaagt tttctttttc ctacctgatg ctgtgcacag catgaccatt aatgaaaaag
     4561 aaaacaccat gatcacagag gcgctcatcg acacggtcgt aaaagacttt gaaacacgcg
     4621 aagtttaccg aatacgcaaa caactataaa aaaaacaggt taggacctcc ctagctttag
     4681 cttgtctagg gctccgcgtt ggtgctagat tgctcacaaa tatgcaatca aaacaggcgc
     4741 ttctgtacac gaataaccgt atcttcaaca atgctcacaa gcataagatt gcggtagata
     4801 ttctagagcg ctacaccggc tccgaagcaa gcatgtttcg taaacaggtg atccttacca
     4861 atttcgatta ctacgtagaa acattcaaaa aaatgtccgg cacggtagca acaaaaggta
     4921 gcgcaatgaa cgctgctcat aacgcccgtc tcgacgtttc aattgttgat tttagcattg
     4981 gcagtcccaa cgctgcactt attatcgaag tattgagtgt tatcgaaccc aacgcagtat
     5041 tatttcttgg aatgtgcgga ggtcttcatc gatcgcttaa agtcggtgat tttattttac
     5101 cgatggccgc gatccgggat gaaggtgcgt cacgccactt catgccttcc caagttccgg
     5161 cacttccaac gtttaaagtt caaaaattca tcagtcagat gatcgtggaa aaaggactag
     5221 attatcgcac cggcgtggtt cacacgaccg attatcgttt ctgggaattc aatcaacgat
     5281 tcaaagaaac tctttacgaa gaacgtgcgc tcgccattga aatggaatgc gcgactcttt
     5341 tcgtagcggg attcgcttca aaggtcccca ttggagcgct tttactggtg agtgacctgc
     5401 cgctccgacg cggtggaatt aaaacaaaaa aatcggctaa agcagtattt aggaaattta
     5461 ctgatgtaca cctggatctc ggcattaaat ccatgagtca aattgctgga cgcggtgaac
     5521 acatacgtca ctacaagtgg taggcggaga gagtttatgt ttaaaaaaat gttcgactgg
     5581 ttatcaaatg atctcgcaat agatcttggc actgcaaata cgttggtgtt cgccaaagag
     5641 cgcggcatta tcataaacga accgtcagtg gtcgctgtgc ataaaaatgc tcgcggtcag
     5701 accaaagttt tagccgtcgg caaagaagct aaagaaatgt tggggcgtac gccaggaagt
     5761 attgttgcga ttcggcccat gaaggacgga gttattgctg acttcgaagt cactcagagc
     5821 atgctgaagt acttcatcaa taagtccaat gacaggcatt catttttacg cccgcgcatt
     5881 attatctgca ttccatttgg gattacccag gtagaaaaac gggcagtaaa agaatccgcg
     5941 caatccgcgg gggcccgcga agtatatcta attgaagaac caatggcggc ggctattggg
     6001 gctggtcttc caatcaccga accatctggc aatatgatcg ttgatatcgg cggcggcaca
     6061 actgaagtgg ccgtgataag tttgggcgga attgtttatt ctaaatcagt gcgtgtcgct
     6121 ggggataaat ttgatgaggc cattgtaaat tatattaaac gtaagtactc gcttctcatc
     6181 ggagaacgca ctgcagaaca aataaaactg gccataggta acgcatatcc gttcgaggaa
     6241 gaaaaaacct atgaagtgaa aggtcgcgac ttaattgcag gggcgccaaa aaccatcgaa
     6301 gttaattcgg aagaaatccg tgacgctcta gccgatccag tctccgccgt aattgacgcc
     6361 attaaaactg cccttgagcg cactccaccg gagcttgccg ctgacattgt ggacaatgga
     6421 attattctcg caggtggcgg cgctttgttg gctaatctcg acattctcat caaggaaaaa
     6481 actggattac cagtggcgct cgccgaagat cctcttactt gtgttgtg
//
LOCUS       DAOMXO010000035         6527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6227_length_6527_cov_5.322002, whole genome shotgun sequence.
ACCESSION   DAOMXO010000035 DAOMXO010000000
VERSION     DAOMXO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6227_length_6527_cov_5.322002"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLH57_01395"
     CDS             <1..207
                     /locus_tag="PLH57_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01294.1"
                     /translation="DLRDKAGGWTVSPTPVFMTELSESDVVLRTLMPVRNAWARYFLL
                     RYDEAGVNSVTVGNRTSKVGLPIR"
     gene            complement(224..1843)
                     /gene="nadB"
                     /locus_tag="PLH57_01400"
     CDS             complement(224..1843)
                     /gene="nadB"
                     /locus_tag="PLH57_01400"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRK01295.1"
                     /translation="MKFDSDVLVIGSGIAGLSYALKAARFGTVHVVTKNRQDEGATRY
                     AQGGIASVFSKSDSFASHINDTEVAGAGLCHRNIVELTVQEGPARVQELVDLGVAFDK
                     DTTSGEYDLTIEGGHEHRRVFHSRDFTGEAIETALVAAVSKHPNIRLFENHAAVDLLT
                     NVRPQKLRRSDGMRCQGAYVLNSTSGAIHVFSARVTVLATGGAGKVYLYTSNPDVATG
                     DGIAMAYRAGARVANLEFMQFHPTCMYHPRAKSFLISEALRGEGGELIDASGAAFMQK
                     YHSMGSCAPRDIVARAIDSEIKRQGADCVYLDITPKRSGHTAAALKERFPNIYAKCRE
                     YGIDLTTEPIPVVPAAHYMCGGVLTDEHGESTIENLFAIGETACTGLHGANRLASNSL
                     LEGAVFAHRAALRTEERLRRMKPKLDLKIYEALPDWDSGKAVPMEERINIAHTWRELR
                     TTMWNYVGIVRTNRRLEKARTRLDYISHEVQEYYWSYLLNPELIELRNLVTVADLIVR
                     CALARKESRGLHANLDTPYLDDTYFKRDTIV"
     gene            1904..3097
                     /gene="alr"
                     /locus_tag="PLH57_01405"
     CDS             1904..3097
                     /gene="alr"
                     /locus_tag="PLH57_01405"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRK01296.1"
                     /translation="MHLGSNVDFTAGITNLAESGPYSPPRRVEARVSVPRFVKNTERL
                     VQLSRKNRPHFLGLIPMVKCDAYGHGAAPLVQALAEKNLRSVIGLGVATLDEALAIRD
                     AGISLPVFVFSDCTPFSEALYRIALEKDFIPILNSLADVQILARVAKKERNSKLKFHL
                     KFNTGMHRLGIPANDSREVARVLWSARLRPNGVCTHLAQASDLDSKISQTQWARFRSV
                     LNTFADVAMIHAESSDAIFSAAKGPWRTVCNVTRPGIGLYGYSASSKTSVSGIEPVMT
                     VRARVITAEVLGKGESIGYGATFRATKKIARSVLAMGYGDGFRRILSNRSVVIDGKIR
                     RVLGRVSMDVTSVEGNFRPGTWVTLLGGSARSAHYMAEQAETNVYELFTALSPRVRRT
                     LRGVK"
     gene            3094..3891
                     /locus_tag="PLH57_01410"
     CDS             3094..3891
                     /locus_tag="PLH57_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK01297.1"
                     /translation="MTLVLWIESIGAAVLRFIEQTGHISKFFAEAVRWLPRSPFRFKL
                     FLEQFLFVANKSLFIVCLTAVFSGMVFAIQIYFGFQVINADALVGPSVAIGFVRELGP
                     VFTGIVVTGRAGAAMAAQLGTMRVSEQIDAMDVMGVNSIHYLVVPRIVASFIALPLLC
                     TLFIFVGNVGSYFTGVYLLNIDPIIYFSHLQEFVEPRDMAQGLIKASAFGLIFSTIGT
                     FKGYFTQGGAEAVGRSTNEAVVITLVLILVSDYFLTLVIRAMLYQGV"
     gene            3894..4703
                     /locus_tag="PLH57_01415"
     CDS             3894..4703
                     /locus_tag="PLH57_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK01298.1"
                     /translation="MASAESAIVIRDVRKSFSRGRYHVFRGLNLNVPKGKITFLLGPS
                     GVGKSVLLKHILGLLKPDSGEVLVGGQAIPYENSRKLAEMRKRFGMLFQNSALFDDMT
                     VGQNVEFPLVEHRKQMTREQRRSKVVEKLLAVGLKPDQTIDKFPNELSGGMRKRVGLA
                     RAIVLEPEFMLYDEPTTGLDPVTRSMVDDLIIETNQKFKLTTIVISHDIPSALYSADY
                     IAFLNDGQIAFFGTPDEFRGCTHPVARGFLESEARHRKAVYTQGEGKGAPT"
     gene            4700..5998
                     /locus_tag="PLH57_01420"
     CDS             4700..5998
                     /locus_tag="PLH57_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRK01299.1"
                     /translation="MTGELKAGLFVLAAIAALVYMTTRLTQNEYSFRGVKTYHAYVSD
                     AAGLLSKTKVKMSGLDVGQLDTVELIERKARVTFQIAADIKVKKDASVAVKSIGFLGD
                     KYLELYPGSEKAPDAEEGATLAESATGGGLEQLTAKTAEVMGNLKEITEMLREALKGD
                     NEFGDGSSRLDRILDNMEQFSAGLASVDRLGDLADRLTEVAENVREVTSKVNRGDGTL
                     GRLINDPETIEKINSTLSGVNKFINKADRMSVLFDARTGVLATTGGSFTKVSLMVQPT
                     FDKYYLLGINTRPQGKTVVTRTETTQSPDAAGAAPSIVEEKETEEDGITFDAQFAKRW
                     GDATFRVGLFESTGGIAADYAFLEDRVRPYAEIYRFKKGQDPQLNVGVTATAYKPFYL
                     WMGGDHILNSRDRNFFVGGGVRFSDQDIKSLVIAGAGAAK"
     gene            5995..6504
                     /locus_tag="PLH57_01425"
     CDS             5995..6504
                     /locus_tag="PLH57_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01300.1"
                     /translation="MTDEKQWLVRKRRGGLESSIKVLGPFWSAQIREMVLNSALELED
                     EVCVENGYWIGLHEREEIKSMLGVDPVARTPKTTDQENTQPDIEATDEITMAGNSPTH
                     VPTIRADDIQIRERGFQRLTAPVVPKMKVVNPQFLGVEKSRYWGILLVLFTLTAIVGI
                     LWLIRVLDI"
BASE COUNT         1541 a         1543 c         1804 g         1639 t
ORIGIN      
        1 gatctacgtg ataaggcggg gggatggacc gtgtcgccga ctcccgtttt tatgacggaa
       61 ctctccgaga gtgacgtggt actgcgaact ctgatgccgg tgcgcaacgc ctgggctcga
      121 tattttttac tgcgctacga cgaagcgggt gtgaattcgg tgactgtggg caatcgcact
      181 tcaaaggtcg gtttgccgat ccgttgaagc gaccctggaa agatcacacg atcgtatcac
      241 gcttaaaata ggtgtcatcg aggtagggtg tgtcgaggtt ggcgtgtaac ccacggcttt
      301 ccttgcgtgc aagtgcgcag cgaacaataa ggtcggccac cgtgacgagg tttcgcaact
      361 caatgagttc ggggttcaag agataactcc aataatattc ctgcacttcg tgagaaatat
      421 agtcgagtcg agtgcgagct ttttcgagcc ggcgattcgt gcgtacgatg ccaacgtaat
      481 tccacatagt cgtgcgcaat tcgcgccatg tgtgggcgat gttaatgcgc tcttccatgg
      541 ggaccgcttt ccccgaatcc caatcgggca gcgcttcgta gattttcaaa tccaatttgg
      601 gtttcatgcg tcgcaaccgc tcttcggtgc ggagtgctgc gcgatgagcg aagaccgcgc
      661 cttctaagag ggaattgctg gcgagacgat tggcaccgtg taggccagtg caagcagtct
      721 cgccgatcgc aaaaaggttt tcgatagtgg actcgccgtg ttcgtcagtg agaactccgc
      781 cgcacatgta gtgagccgct gggaccaccg gaataggttc agttgttaaa tcgataccgt
      841 attctctgca tttggcataa atgttgggaa aacgctcttt taatgcagct gcggtgtgac
      901 cagagcgttt tggcgtgatg tcgagataga cgcaatcggc gccttgacgc ttaatttccg
      961 aatcgatcgc gcgagcaacg atgtcgcgag gagcgcagga ccccatggag tgatactttt
     1021 gcatgaaggc cgccccgcta gcatcgatca attcgcctcc ctcgccgcgg agtgcctcac
     1081 tgattaagaa agacttagct cgtgggtgat acatacaagt cggatgaaac tgcatgaact
     1141 cgaggttcgc tactcgagcc ccggctcgat aagccatggc gattccgtct ccagtcgcga
     1201 cgtccgggtt cgacgtatat aaatacactt tgccggctcc gcccgttgcg agcacggtga
     1261 cccgcgccga aaaaacgtga atggcgccac tggtgctatt gagcacgtag gccccctgac
     1321 aacgcatgcc gtcagatcgt cgaagttttt gggggcgtac gttggtcagc aagtctactg
     1381 cagcgtgatt ttcaaaaagt cgaatgtttg gatgtttact gaccgctgcc accaaagcgg
     1441 tttcgatcgc ttctccggtg aaatcgcgag agtggaatac acgtcggtgt tcatgtccgc
     1501 cctcgatcgt gagatcgtat tcaccgctag tagtgtcctt gtcgaacgcg actcctaaat
     1561 ccactagttc ttgtacacgc gcgggcccct cttgcacggt cagttccaca atgtttcggt
     1621 gacacaatcc agcaccggca acttcggtgt cgttaatatg actggcaaac gaatcactct
     1681 tcgaaaaaac cgacgcaatt ccgccttgcg cgtaacgcgt cgccccttcg tcctggcgat
     1741 tttttgtgac gacgtgaacg gtaccaaacc gggccgcttt gagagcgtag ctaagtcccg
     1801 caattccgga accgatcacg agaacgtcgc tgtcgaattt catcgtggcg atttaacaca
     1861 agacagtagc tcggacgatt aaaatcgggc aaactagagt agaatgcacc tgggatcgaa
     1921 cgtcgatttt actgccggaa ttacgaacct cgcggagtct ggcccttatt ccccccctcg
     1981 ccgcgtggag gcgagggtat cggttcctcg tttcgtcaaa aatactgagc gattagtgca
     2041 gttaagtcgt aaaaaccggc cacacttctt gggcttgatc ccgatggtta aatgcgacgc
     2101 ctatggtcac ggcgcggcac ctttggttca agcattagct gaaaaaaacc tgcgttcggt
     2161 gattggtctg ggcgtcgcga ctcttgacga ggccttagcg attcgagacg ccggcatatc
     2221 tttacccgtt ttcgtgtttt cagattgcac ccctttttcc gaggcgcttt atcgcattgc
     2281 gctcgaaaag gattttattc caattttaaa ctctctggcc gacgttcaaa ttttagcccg
     2341 ggttgcaaaa aaggaacgaa actccaagtt aaagtttcat cttaagttta atactggcat
     2401 gcaccgtctc ggaattccgg caaacgacag tcgcgaagtg gcgcgagtgt tatggtcggc
     2461 ccgtttgcgg cccaacggag tttgcactca tctcgcgcag gcctcggatt tagattccaa
     2521 gatatcgcaa acccagtggg cccgctttag atctgttttg aatacgtttg cggacgtagc
     2581 tatgattcat gccgaaagta gcgacgcaat ctttagcgcg gccaagggac cgtggcgcac
     2641 agtgtgtaac gtcacgcgtc ccggtatcgg attgtatggt tacagcgctt caagtaaaac
     2701 ttcagtttca gggattgagc cagtaatgac ggtgcgggct cgcgttatta ccgccgaggt
     2761 gctcggcaag ggtgagagta ttggttatgg cgcgactttt agagctacga aaaaaatagc
     2821 ccgtagcgtg ctggccatgg gttatggcga tgggtttcgc cggatcttgt cgaatcgaag
     2881 cgtagtgatc gacggtaaaa ttcgcagagt attagggcgg gtgagtatgg acgtgacgtc
     2941 ggtcgagggg aactttcgcc cgggcacttg ggtcacgctt ctaggcggta gtgcgaggtc
     3001 tgctcattac atggccgagc aagctgaaac aaacgtttat gaattattca ccgcgctttc
     3061 gccgcgtgtg cgtcgaactt tgagaggtgt gaagtgacgc tggtactatg gatcgaatcg
     3121 atcggtgcgg cagttctcag gttcatcgaa caaacgggtc atatttccaa gttttttgct
     3181 gaagccgtgc gatggttgcc gcgatcacca tttcgcttca aattattttt agagcaattt
     3241 ttatttgtag ccaataaatc gctgttcata gtttgtttga ccgcggtttt cagcggtatg
     3301 gtgttcgcaa ttcaaattta tttcggcttt caagtgatta acgctgacgc gttggtggga
     3361 ccgtcggtag cgattggatt tgtccgagaa ttagggcccg tttttaccgg gatagtggtt
     3421 actggtcgtg ctggtgcagc catggcagcg caactcggga caatgcgcgt gagcgaacag
     3481 attgacgcaa tggatgtaat gggcgtgaac tccatacact accttgttgt gccgcgaatc
     3541 gtggcaagtt ttattgccct tccgcttctt tgcacgctct ttatttttgt aggcaacgtt
     3601 ggaagttatt ttacgggcgt atatttgctg aacattgatc caattattta tttctctcac
     3661 cttcaagagt tcgttgaacc acgagacatg gcacagggac ttattaaagc gtcggcgttc
     3721 gggttgattt tttcgaccat cgggaccttc aagggttact ttacgcaggg gggcgcggag
     3781 gctgtcggaa ggtcgaccaa cgaagccgtg gtgatcacct tggtgttgat tctcgtgagc
     3841 gactacttct taacactcgt catacgagcc atgctttatc agggggtttg atcgtggcgt
     3901 ctgctgaatc ggccattgta attcgcgatg ttcgaaaaag tttttcgcga ggccgttatc
     3961 atgtttttcg cgggctcaac ttaaatgtgc ctaaaggaaa gattacattc ttactcggac
     4021 cctccggggt aggaaaatct gtcctactaa aacacatttt agggctcctt aagcctgatt
     4081 caggagaggt tttggttggc gggcaggcga tcccgtatga aaatagtagg aagttggcag
     4141 aaatgagaaa gcggttcgga atgttgttcc agaattccgc actgtttgac gacatgacgg
     4201 tcgggcagaa cgtggagttt ccgttggtcg aacatcggaa acaaatgacg cgcgaacaga
     4261 ggcgaagcaa agtggtggaa aaattattgg ccgtaggact aaaaccagat cagacgatcg
     4321 ataagttccc caatgaactt tcaggcggca tgcgtaagcg tgttggtctc gcgcgagcaa
     4381 ttgttctgga acctgagttt atgctctatg atgaaccgac tacaggactg gatcccgtca
     4441 cgcgatcaat ggtcgatgat ctgattatcg aaacgaatca aaagttcaaa cttactacga
     4501 ttgtgatcag ccacgacatt ccatcagcat tgtattcggc cgattacatc gcatttctca
     4561 acgacggaca gatcgcattt tttggaacgc cagatgagtt tcggggctgt acgcacccgg
     4621 tcgcgcgagg atttctagaa agtgaagctc gccatcgtaa ggccgtttat acgcagggtg
     4681 aaggaaaggg ggccccgaca tgacgggcga actgaaagcc ggactgtttg tactcgcagc
     4741 gattgcggcc ttggtgtaca tgaccacgcg actcacccaa aatgagtatt cgtttcgagg
     4801 ggtgaaaacc tatcacgctt acgtgagtga cgcggccggt ttgctgtcga aaaccaaagt
     4861 gaaaatgtct gggctcgacg tgggccaact agacactgtc gaactaatcg aacgaaaggc
     4921 tcgcgtaaca tttcagatag cggccgacat caaggtaaaa aaagatgctt cagtcgctgt
     4981 gaagtcgatc ggatttttgg gcgacaagta tttagagctt tatccagggt ccgagaaggc
     5041 acccgatgct gaagaaggag ccaccctagc tgaaagcgcc actggtggcg gacttgagca
     5101 actgacagcg aaaacggctg aggtcatggg taatctcaaa gaaattaccg aaatgttgcg
     5161 ggaagcctta aagggcgaca atgagtttgg tgatggcagc tcgcggctcg atcgcattct
     5221 cgataatatg gagcaatttt ccgccggact tgcgtccgtc gaccgcctcg gggatttggc
     5281 cgatcgtttg accgaagttg cggaaaacgt ccgcgaagtt acttcgaagg taaatcgtgg
     5341 agatggcact cttgggcgat tgatcaacga ccctgagacg attgaaaaaa ttaattccac
     5401 gctctcgggt gtgaacaagt tcatcaataa ggcggatcgc atgtcggtgc tcttcgacgc
     5461 acgcactggt gtgctcgcaa ccacgggcgg ctctttcacc aaggtctctt tgatggtgca
     5521 gccgactttt gataaatact accttttagg aattaacact cggcctcaag gtaaaaccgt
     5581 agtgacccgt actgaaacta cgcaaagtcc cgatgccgct ggcgctgccc cttcgattgt
     5641 cgaggaaaaa gaaaccgaag aagatggcat cacgttcgat gctcagtttg ccaaacgctg
     5701 gggcgatgca acctttcgtg ttggtctatt tgagagcaca ggtggcatag cagctgatta
     5761 tgcctttctg gaggaccgcg tgcggcccta tgccgaaata taccgtttca agaaaggtca
     5821 agaccctcag ctcaacgttg gcgtcaccgc aacggcctac aaaccatttt atttgtggat
     5881 gggcggcgac catattttaa attcgcggga tcgtaatttc ttcgtcggcg gcggcgtgag
     5941 gttttcggac caagatatta agagtctggt tattgcgggc gcgggagcag cgaagtgaca
     6001 gacgaaaaac aatggcttgt tcggaaacgt cgaggtggtc ttgaaagctc catcaaggtt
     6061 ttgggtccgt tttggagtgc ccagattcgt gaaatggtgc tcaatagtgc gctggaactt
     6121 gaagacgaag tctgcgttga gaacgggtat tggattggtc tgcatgagcg ggaagaaatt
     6181 aagagcatgc tcggggtaga cccggtggct cgcactccca aaactacgga tcaagagaac
     6241 actcaacccg atatcgaagc caccgacgaa attaccatgg caggtaatag tcccactcac
     6301 gtcccgacca ttcgcgccga cgatatacaa attcgcgaga gaggttttca acgcttgacc
     6361 gcacccgtgg tgccaaaaat gaaagtggtc aatcctcagt ttttaggggt ggaaaaaagc
     6421 cgttactggg gaatattgct ggtgttattt actcttaccg ccatcgttgg aatactttgg
     6481 ctgattcgcg ttctagatat ttaaatgaaa ttcctccctg gggcttt
//
LOCUS       DAOMXO010000036         6513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6248_length_6513_cov_5.622019, whole genome shotgun sequence.
ACCESSION   DAOMXO010000036 DAOMXO010000000
VERSION     DAOMXO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6248_length_6513_cov_5.622019"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLH57_01430"
     CDS             <1..286
                     /locus_tag="PLH57_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRK01301.1"
                     /translation="NPSWFAFPMHCAEGLEREKITTYLEQHLVGTRLLFGGNILKQPA
                     YKKSSFRVVGDLKNTDAIMRDTFWIGTHPAISDKHITYMLEQLEQAVRSF"
     gene            complement(289..1245)
                     /locus_tag="PLH57_01435"
     CDS             complement(289..1245)
                     /locus_tag="PLH57_01435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK01302.1"
                     /translation="MGSNPTLSAIQILRVSMDIVSLVYNRENDVKNLLDSIQIQSEMP
                     KNVWLIDNGSNRPLTIDSSKYSFPVHVVRIENNIGIAGYNLGFKKSQAKYVMAVDSDV
                     TLDRNAIKVFKKAIQDYPDLVVGGARILDATTQTTMFDNPIHAEPELPGGGQQMVQFN
                     GCCFLVLREAFLGLGGFDQRLFIYVNEWDFTLRVFNRFRMDQIRYYSEAVAYHKTAPS
                     SDRAKLYDALIRRNEFWVRWKYYPLGSALNYSARFYVGSIKRMILGGPKDFKLFGGYL
                     LSGTQGLTWALRERQELPSAVFEQLMKMRDTCEQPENLVRNI"
     gene            complement(1218..1307)
                     /locus_tag="PLH57_01440"
     tRNA            complement(1218..1307)
                     /locus_tag="PLH57_01440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1271..1273),aa:Ser,seq:gga)
     gene            1410..2300
                     /locus_tag="PLH57_01445"
     CDS             1410..2300
                     /locus_tag="PLH57_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRK01303.1"
                     /translation="MSLSPHFGVRFAPSPTGRFHVGNLRTAWISHSLARVRQQPWIVR
                     FEDIDTPRVLPGAMDQQLSDLATLGMVPDTIVVQSKLLNRHKDVFSLAVNSGQVYPCT
                     CSRAEVQRALANLASAPHEIEPLYDGTCRATRWDPVQILKRPENRQIAWRFRSPPDSG
                     HQDFIVARTTGGISTVAPSYHFACAIDDYDGNYGLLVRAWDLAPARLIQKAIYDWLSS
                     KEIPRVYPEIFHTSLVTDNDGRRLEKRTKGVTLEELLASGHDVGGLILLFERSFAVVG
                     PLSDGERHQQITLSSLGIVN"
     gene            2348..2935
                     /locus_tag="PLH57_01450"
     CDS             2348..2935
                     /locus_tag="PLH57_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01304.1"
                     /translation="MNQSLRKKTVGLFLVLINLLCMHSSAQQKDKTILGMISQRNAQS
                     DQQANLTQGFFEVGGSLGFHNTFKNGDDYSHLILSFVSEYFVIDRLSLGGQFSHYSTR
                     GFNETAIGPSGKYYFFQKDSFAPFGGLAVLYNNSNDFRYYFSTKATFGFKYFVTPAVG
                     IVSSLSWFHLFGGNRFDSRATLNRVSLEMGLSVHL"
     gene            complement(2886..4205)
                     /locus_tag="PLH57_01455"
     CDS             complement(2886..4205)
                     /locus_tag="PLH57_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRK01305.1"
                     /translation="MKRNFLAWSLHTFWTLAEHASTRILDAIVTIVLIRIFSTEQFGL
                     FSIYQSWTGIALLFLPSLELALYRDYNRLKNQGSLARELSVYRQFNYIKMFVALMFAF
                     AISLIPNDMNWTVRFAIVIFAFALPLSQAFYGFLREILRFELRTKWVALIGAVQRLVL
                     IGAIIGVARLRPGDASAISGTAILIYVLFGWVWGVPLRGAFKVSKENFGKYLQRMKAI
                     LSSTVLWIHLNGVMAQFIQTLDTFFLGNLGVALETVGVYSITLKSANFFQVLPVALVN
                     PFGVYLGRNAGNASFLEEAKLLKKMILGFVTLCGSLYLFGHFFALEILNAVGRGKISV
                     ENVDLALTLFRWQLAGVLLLCVTHPLTTYIGARCSLKAISKEIFLPWLIVAAIIYGVA
                     AKFGGALWSAYANVPTYLILVVLLGRFYRRQVTGEPTSPFQGKPGLM"
     gene            4230..5933
                     /locus_tag="PLH57_01460"
     CDS             4230..5933
                     /locus_tag="PLH57_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01306.1"
                     /translation="MTKFKLFHSLNQNKIEFLCLAALTMAMVPLLLTGHYFCSDWLNH
                     LWPVGYISNYFRSHLWIPDVVSTNNFIGLPYHVFYGRWLYIFGGLLGALFQPEVGLRL
                     VLFFSFFFQAASVFLAFRSLGHQRDTSAFATVVVSISTYSLTNLYHRAALPEYVATAS
                     IITTIALTVLLANTRQTPHKIFYFFSLVLALVWATGNHPITTLYFGLVASLLFLVLQI
                     FAPSLIKNNFRILLILSFAGAIAFAINFSSYYAIIRYKSDLHISTTSILNFIESVDTI
                     VTRLFPIPFDKRSLINGTKIDTPYLETQINGALLLLVLLGIRTKTLKPFKFACLGLAG
                     IFLWLSVSNTGWNFIPSSLTIVQFPYRLVTYVNLFLFLALLGTNLEKHRRTIAPLMVY
                     ILIGLIIKLVHVGAVKTDKSKVGVGNDKSYLTWINDTGPLKNYTTTGPWSQIDNADLK
                     TAKTTNLTITVNSPHFGEVPSALAAESGWHLTNVSAFPWNHIYVNGERAKDLLRNDGR
                     IAVHISQSKSRLDLAVTPDKTWYRLNIISYLFLIAALTGFVGSFILLLRSMLRTKGLW
                     A"
     gene            complement(5884..>6513)
                     /locus_tag="PLH57_01465"
     CDS             complement(5884..>6513)
                     /locus_tag="PLH57_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01307.1"
                     /translation="KSFGPGVIFILLPLSQKKIWNTLSQAQRALVAIGAATVVIFTLF
                     INMWGPFDSWFYLHVIIPFGWAFIVAGLVRSKFLVTRVGFVFLALTIVYQVISVVNGS
                     KNYYDYFERSRQVADYACHEWRKNPHIKLVSNCHEGSQQLSRYLLGENKCASDFKVDY
                     LRTTESEFDFFANTSKNTLVLYSRYCDNWVSLGFRPKGLSFGAWTVVAR"
BASE COUNT         1818 a         1579 c         1297 g         1819 t
ORIGIN      
        1 caatccgagt tggtttgcct ttccaatgca ttgtgctgaa ggtttagagc gcgaaaaaat
       61 cacgacctat ttggaacaac accttgtggg cacgcgtttg ctttttggcg gcaacatact
      121 gaaacaaccc gcatacaaaa aatcttcgtt tcgtgtcgta ggcgacttaa aaaataccga
      181 cgccatcatg cgagatacct tttggattgg aacccatcca gcgattagcg acaaacacat
      241 tacttatatg cttgaacaac tcgaacaggc ggttcgtagt ttttagcttt aaatatttcg
      301 gactaaattt tcaggctgtt cacatgtgtc gcgcatcttc attagctgct cgaaaaccgc
      361 actcggaagt tcctgtctct ctcgaagagc ccaggtcaat ccctgggttc cgcttaagag
      421 gtacccgccg aatagtttga agtccttggg gccacccaaa atcatacgtt taatggatcc
      481 gacataaaat ctagccgagt agttaagcgc tgaacccaat gggtaatatt tccaacgaac
      541 ccagaattca tttcttcgaa ttagagcgtc gtacaacttt gctcggtcac tcgaaggggc
      601 ggtcttgtga taggcaacag cttcagagta gtatcgaatt tgatccattc gaaaacgatt
      661 aaagactcgt aaagtaaagt cccattcatt cacataaata aagagccgct gatcgaaccc
      721 ccccaggcct aaaaatgcct cgcgaagtac cagaaaacaa caaccgttaa attgcaccat
      781 ttgttgcccg ccgccgggca gctcgggctc tgcatgaata gggttatcga acatcgtagt
      841 ttgggtagtt gcatctaata ttctagcacc accaaccact agatctggat aatcctgaat
      901 cgctttttta aacactttta tcgcatttcg atcaagcgtt acgtcactat ctacagccat
      961 tacatatttt gcttggctct ttttaaatcc cagattgtaa cccgcgatgc cgatattgtt
     1021 ttctattcgg acaacatgta ccggaaagga atacttcgaa ctatcgatcg taagtggcct
     1081 attgctgcca ttgtcgatta gccaaacatt tttgggcatt tcgctttgaa tttgaatcga
     1141 atcgagcaaa ttttttacat cattttctcg attgtaaact agactcacga tgtccataga
     1201 aactcgtaga atttgaatgg cggagagagt gggattcgaa cccacgttac gtgttaccgt
     1261 aaacacgctt tccaagcgtg cgccttcagc cactcggcca tctctccgca aaaaggcaaa
     1321 accaacgctt aacttttgac ccaaccagaa gcaagtgggt tctcaacaaa aatctataaa
     1381 acagccggtc cgttagttag tatcgatcta tgagcctgtc ccctcatttt ggcgtccgat
     1441 tcgccccttc gcctaccgga aggtttcatg ttggcaacct acgaaccgcc tggatttccc
     1501 actctttggc ccgagttagg cagcaaccgt ggatcgttcg gttcgaagac atagatactc
     1561 cgagagtttt acctggagct atggatcaac aactatcaga tctcgcaacg ttgggtatgg
     1621 tgcccgatac catcgtcgtt caaagcaagc ttttaaaccg acataaggac gtattttcct
     1681 tggccgttaa ctcggggcaa gtttacccct gcacttgttc acgcgcagag gtacagcggg
     1741 ctctggccaa tcttgcctct gcaccgcacg aaattgaacc cctctatgat ggtacctgtc
     1801 gggctacaag atgggatcca gtacaaatat taaaaagacc tgagaaccgc caaatcgctt
     1861 ggagatttcg aagtccaccg gattcggggc atcaagactt catcgtagcc agaacgaccg
     1921 gaggcatctc taccgtagcg ccgtcctacc atttcgcgtg cgctatcgac gattacgacg
     1981 gtaattacgg actcctcgta cgggcctggg acctggctcc ggcccggctg atacaaaaag
     2041 caatctatga ttggctctcg tcgaaggaaa taccccgggt ttaccctgaa atttttcaca
     2101 cttctttggt cactgataat gacggtcgac gccttgaaaa aaggacgaaa ggggtcacgc
     2161 tcgaagaact attggccagt ggacacgacg taggcggtct tatcctgctt ttcgaacgtt
     2221 cttttgcggt ggtgggcccg ttgtccgacg gcgaacgtca tcagcaaatc accctcagca
     2281 gcctcggtat cgtaaactaa tcgctttgct tgacaccgat catagttcaa atcataactg
     2341 gaccttaatg aaccaatctc tacgcaaaaa aactgttggc ctatttttgg tactcattaa
     2401 ccttctgtgc atgcactctt cagcgcagca aaaagataaa acaattttag gaatgatctc
     2461 ccaacgaaat gcccaatccg atcaacaggc caacttaact cagggttttt tcgaggtcgg
     2521 cggcagtctc ggatttcata acaccttcaa aaacggcgac gattattccc acctaattct
     2581 gtcttttgta tctgagtact tcgtaattga tcgactttcc ctgggcggac aatttagcca
     2641 ctattcgaca cgtggcttta acgaaacggc cattggtccc tctggcaagt attacttttt
     2701 tcagaaggat tccttcgctc ctttcggagg acttgcagtc ctttacaaca actcaaacga
     2761 ttttcgttat tacttcagta cgaaagcaac ttttggtttt aagtattttg tcacacccgc
     2821 ggttggaatt gtaagcagtc tatcttggtt tcatctcttt ggtggcaatc gctttgattc
     2881 gcgcgctaca ttaaaccggg tttcccttga aatgggcttg tcggttcacc tgtaacctgt
     2941 cgtcgataaa atcgcccgag taacaccacc agtattagat aagttggaac attggcgtaa
     3001 gcgctccaaa gtgctccacc gaatttcgcg gctactccgt aaataatcgc agctacgatt
     3061 agccagggca ggaatatttc tttcgatatg gctttaagcg aacagcgtgc acctatatag
     3121 gtggttaatg gatgggttac acacagaagc agtacgcccg ccaattgcca gcgaaatagt
     3181 gttagcgcta aatccacatt ttcgacgcta atttttccgc gacctacggc atttaaaatt
     3241 tctagggcga agaaatgccc aaaaaggtac aaactaccgc acagggttac aaaacctagg
     3301 atcatttttt ttaaaagttt tgcctcttct aaaaaagatg cgtttccagc gttgcgcccc
     3361 agatacactc cgaacggatt gaccaatgct accgggagaa cctggaaaaa attcgcgctc
     3421 tttaaagtaa tggaataaac acccacagtt tccaatgcaa caccgagatt ccctaggaaa
     3481 aaagtatcaa gtgtttgaat aaactgagcc attactccat ttagatgtat ccacagtacc
     3541 gtggaagaaa gaatggcttt catccgctgc aaatattttc cgaaattttc ttttgaaact
     3601 ttaaatgcgc ctcttagcgg cactccccag acccacccga atagtacgta gattaatatc
     3661 gcggttcccg atatggcgct tgcgtctccc ggccgaaggc gtgcgacacc aattatagct
     3721 ccaataagca ccaatctctg cactgcgcca attaaggcta cccacttcgt tcttagttcg
     3781 aatctaagta tctcccgcaa aaaaccgtaa aacgcctgtg agagcggcaa ggcaaaagca
     3841 aaaatcacaa ttgcaaatcg tactgtccaa ttcatgtcat tcggaattaa ggaaatagcg
     3901 aatgcaaaca tcaacgctac aaacatctta atataattaa actgacgata aaccgagagc
     3961 tcccgcgcca atgaaccctg gttcttgagc ctattgtagt cacgatagag cgccaactct
     4021 aacgagggca aaaacaagag tgcaatacca gtccaacttt gataaattga aaaaagacca
     4081 aactgttcag tggaaaaaat ccgaatgagc acaatcgtta caattgcgtc caatattcga
     4141 gtgctcgcgt gctccgctag cgtccaaaag gtatgcagcg accaagccaa aaagtttctc
     4201 ttcattctta atttgagact cgcacaggca tgactaaatt caaactgttt cactcactta
     4261 atcagaataa aattgaattt ctctgcctcg ctgcccttac tatggccatg gttcccctgc
     4321 tgctcaccgg tcattacttt tgttcggact ggctaaacca cctgtggccc gtcggttaca
     4381 tttcgaatta ctttagatca catctgtgga tccccgatgt agtttcaact aataatttta
     4441 tcggactacc gtaccacgtt ttttatggac gatggctcta cattttcggt ggactactcg
     4501 gtgcgctttt ccaaccggaa gtcggacttc gacttgtttt atttttttcg ttttttttcc
     4561 aggccgcgtc tgtatttctc gcatttcgca gcttaggaca ccaacgtgac accagcgctt
     4621 ttgcgacggt tgtcgtgagc atcagcacct actcgctcac aaatctatac caccgagcag
     4681 ctttaccaga gtatgttgca accgcgtcaa taattactac gatcgctctt acggttctat
     4741 tggccaatac tcgtcaaaca cctcacaaga ttttttactt tttttcctta gttttggcct
     4801 tggtttgggc aaccggaaac catcctatta ctacactgta ttttggatta gttgcatccc
     4861 tactattttt agtacttcaa atttttgctc ccagtctaat caaaaacaac tttcgaatcc
     4921 tgctgatttt atcttttgcg ggggcaatag catttgctat taatttttca tcatattacg
     4981 caataattcg ctacaaatca gatcttcata tttctactac ttcgatactt aattttattg
     5041 agtcagtaga tacgatcgtc actcgtttgt ttccgatacc gtttgataaa cggtctttaa
     5101 ttaatggcac taaaattgat acaccctatc ttgaaactca aattaacggc gcactactgt
     5161 tgttagtcct ccttggaatt agaaccaaaa ccttaaaacc atttaaattt gcgtgtcttg
     5221 ggctggctgg catttttttg tggttatcgg tgagtaatac aggctggaat tttataccga
     5281 gcagtttgac catagtacag tttccctatc gcctagttac gtatgtgaat ctttttctct
     5341 ttttggctct cttaggaaca aacctagaaa aacataggag gaccattgct cctcttatgg
     5401 tttatattct gataggtctg attattaaat tagttcatgt aggtgctgta aaaactgaca
     5461 aatccaaggt gggagtaggc aatgataaat cttacttaac ttggatcaac gataccggcc
     5521 ctttaaaaaa ttacaccacc actggccctt ggtctcaaat agacaatgca gatttaaaaa
     5581 ccgcgaaaac aacgaatcta acgataacgg taaacagccc tcattttggt gaggttccat
     5641 ctgccctggc cgcagaatca gggtggcatt tgactaatgt ttcagccttt ccttggaatc
     5701 acatttacgt caatggagaa cgagcaaagg accttttaag aaatgatgga agaattgcag
     5761 ttcatatttc ccaatctaag tcacggttgg atctagcggt aacccccgac aaaacctggt
     5821 accgattgaa tataatatcg tatctttttt taattgcagc actaacaggg tttgtcggca
     5881 gctttatctt gctactacgg tccatgctcc gaacgaaagg cctttgggcc taaaacccaa
     5941 actcacccag ttgtcacaat agcgactgta taaaaccaaa gtgtttttgg atgtatttgc
     6001 aaaaaaatca aactcactct cggtggtgcg cagatagtcg accttgaaat cagaagcgca
     6061 tttattttcg cctaatagat aacgactgag ttgctgcgag ccttcgtgac agttggaaac
     6121 caatttaatg tggggatttt ttcgccattc gtgacatgca taatcagcaa cctgcctaga
     6181 acgttcgaag taatcgtaat agtttttact accattcacg acgctgatca cctggtaaac
     6241 gatcgttagt gccaaaaaca caaatccaac gcgagtaact aaaaacttcg acctcaccag
     6301 gccagctact ataaaggccc aaccgaatgg aataattaca tgaagataga accacgaatc
     6361 gaacggtccc cacatattaa taaacaacgt aaaaattact acggtagccg caccaatcgc
     6421 cacaagagct ctctgtgcct gcgacagcgt gttccagatt tttttttgac ttaacggtag
     6481 caatataaaa attacgccgg gaccaaagct ttt
//
LOCUS       DAOMXO010000037         6485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6288_length_6485_cov_4.630793, whole genome shotgun sequence.
ACCESSION   DAOMXO010000037 DAOMXO010000000
VERSION     DAOMXO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6288_length_6485_cov_4.630793"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLH57_01470"
     CDS             complement(<1..280)
                     /locus_tag="PLH57_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRK01308.1"
                     /translation="MTSAVKPDRNSTISVAVVGSPNSGKTTLFNSLTGSRQKTGNYAG
                     VTVEKKEGLLTLTDGRTVQLIDLPGTYSLEPRTPDEELTTQILAGVRTD"
     gene            complement(277..531)
                     /locus_tag="PLH57_01475"
     CDS             complement(277..531)
                     /locus_tag="PLH57_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRK01309.1"
                     /translation="MTALPLLTISDLRPGEQCRIKNLSRIDDRKFARRLEEMGLTCGA
                     TLELSHLAPLGGTIAVRVRSTLIALRTEDAACIEVERTNS"
     gene            complement(528..857)
                     /locus_tag="PLH57_01480"
     CDS             complement(528..857)
                     /locus_tag="PLH57_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRK01310.1"
                     /translation="MRLQNIQSKELAKMRSQRVFLLLAETFQAMGDSSRIQIVWALSR
                     GEQSVTALAQMLDISQPAVSHHLRTLRNLKLVHVRRQGKTSFYSLDDQHIENLLIEGL
                     KHVEDLI"
     gene            992..2302
                     /locus_tag="PLH57_01485"
     CDS             992..2302
                     /locus_tag="PLH57_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01311.1"
                     /translation="MNSNSYSVLCSSIKTGIGLAHGRCAMIRYGIFALVFWTFLTGTA
                     RASLDPLPFLAVDAETAQAIEADIDRMLLEQKENLDITYMTKKWLSKDDPTMTQERFE
                     AAVAWIANDPYGRQVVIDVMKRARVKEVAGIMPYVTVQELQARQWMIDASDENRTIEA
                     MGRTQYSYTVPTSLTPDLERFMAIESLRLASEPNLTMEEQEQLYQPLLLEMIEKLTKS
                     DGLIRDLFFARTNTDLSLVYGMEVPRILVKPNVSMRTAVIAFAHEFVHFAYATFRERL
                     TLIDYDNEEDAAFAFVDGQGEEVDAHIFEFGLNSRMYKNPYAIQKEIRDLFDSDAVFV
                     GDRRKFAKFILEEKSYQKSFEREYFNALLTSYKFEYKALKKLEGLYLIRETEEEQYQK
                     KGNDRKANAALASKVRIRKTIEQYEERLKKYEDKLGIVRKKKSA"
     gene            complement(2385..3431)
                     /locus_tag="PLH57_01490"
     CDS             complement(2385..3431)
                     /locus_tag="PLH57_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01312.1"
                     /translation="MMLTSFSLNRWLRSVIFLSGMCSLISCSSLSPAKRGTHLAINGT
                     KFDRSVELKHFFKGNLHTHSQESDGDAPLNTVVEWYKRNGYDFLSMTEHDKLVDTSSH
                     STSKFITLPGDEITGRSYRVPIHVNAICGKSQLKGIRENRQPGAILQDQINTARADGA
                     LTIVNHPNFGWAFGAKILRKVGDFDFLEVTSGHPLVNENGGEKGISHEALWDDYLTHR
                     GSVWGVGVDDSHHYSDFDIATKANPGRAWVQVAAHRLTATEVCSALQRGDFYFSRGPV
                     ITRLNVKGPTFTLVVSNWNSSGTVEFLGESGRTLNTTRTMPAVYQINGSEGYVRARIT
                     LNGARAWTQPYFIQ"
     gene            3464..4549
                     /locus_tag="PLH57_01495"
     CDS             3464..4549
                     /locus_tag="PLH57_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK01313.1"
                     /translation="MKDFFFQIIRSDEGCRSYVLADSGTKRGLVWDPSLAGLESTLEV
                     VAEQGLTHLILADSHTHADHFSATAELAKRLRDVAKVEIVMGEKTESERPGRRLRDGD
                     RLDMGPKMQAVVLATPGHTEDSICLYIEVGGEKLVLTGDSLLIGSSGRTDFPGASAEA
                     LYHSLHEKIMKLPGTTWLFPGHDYSGYLFSRLEVEAKQNSQLAMDKSEFLLFKQNEVL
                     DCTPVVPKIVDFNRASSPSAPMHIPAAAICARSEPVPGVRSIGTDELRSLIGEHSKKS
                     LFIDVREPDEVNKARIPGLQNIPLSLVWTKLPEIAASESAYFSCQSGRRSGMVTATLA
                     RLGFSNAINVAGGIQGWMARGYHVEAS"
     gene            complement(4546..5190)
                     /locus_tag="PLH57_01500"
     CDS             complement(4546..5190)
                     /locus_tag="PLH57_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01314.1"
                     /translation="MYTINLIGYYGGLLNIHLTHYKSRGDRAFFVIGLVLTGLLSTGL
                     LRQVHARGEKKSSTLSVLGKAYIDMYEKTQALGINPPQSERERVRKESMAPYFKARTE
                     ANNEVVKKYSLLADKLQKNMTDRAENFQKMKRNELSGGAKSKEPTERAYIEDVIGGKM
                     ISVPTKSRAPSSTTTTTTRVNEAGASVGEATGADSVDFGTPSQNGAGPEEPNFN"
     gene            5240..>6485
                     /locus_tag="PLH57_01505"
     CDS             5240..>6485
                     /locus_tag="PLH57_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01315.1"
                     /translation="MPNNIRLLFGVMGLFLSTIVPAQEIQNALDNGPSREDQLDRAAG
                     MPTKSELDYRTLTEAKLSCRNKGSQSFLGSKKCGNVGSELGLKGEIQKRFKDSMKAYA
                     KNYYKGVIYTDLSAPDGAPRNIEVPVCNLSVGEGEGAANQNYKGDSCLDLARLTVDTS
                     YTSASAKVTANLLNGRVKEFAWWRGLYLQVYFNSAGKVMREFEEQGKVTLTRLPDGSV
                     PTGPIARDALRLQIGRDRVIREIASIKGSDQEELGYARVAETKCEARDDKITSHACRL
                     SMVNEHEVAQWTYLMMAEANIRTQVEVDRQFENMETLLEGFQLRLEPICKNQYDADCT
                     LCRDSTRTRRTNRCYQEQMPNMMKQELARYPTDNLLPIPYEELKREVGDQASSSKKDA
                     GSSSPFQFGDVSNLLALSLLGFR"
BASE COUNT         1691 a         1439 c         1694 g         1661 t
ORIGIN      
        1 cgtcggtacg aactccggcc agaatctgag tggtgagttc ttcgtcgggg gtgcgaggct
       61 caaggctgta agtgccaggt agatcgatca actgtacggt acgcccgtcg gttaacgtga
      121 gtagcccttc ttttttttca acagttacac ctgcgtagtt gccagttttc tgacggcttc
      181 ccgtgagact gttgaaaaga gttgtcttgc cggaattggg agatccaacg accgcgacgg
      241 agatcgttga gttgcgatcc ggttttactg ctgaagtcat gagttggtcc tttcaacctc
      301 aatgcatgcg gcgtcttcgg ttcgaagagc aatcaaagta gatcgtacac gaacggcgat
      361 cgtgccgccc aacggagcga ggtgggaaag ctcaagtgtg gcgccacagg tgagacccat
      421 ttcttcgagt cgacgggcaa atttgcgatc atcaatgcgc gaaagatttt taattcgaca
      481 ctgttcccct ggacggagat cgctgatcgt gagcaacggc aaagcggtca tatcaagtcc
      541 tctacgtgtt ttaatccctc aataagcagg ttctcgatgt gttggtcatc cagtgaataa
      601 aaactggtct tgccttggcg gcgcacgtga acgagcttta aatttcgaag cgtacgcaga
      661 tgatgtgaaa ccgcgggctg gctaatgtcg agcatttgcg ccaaagcagt gacgctttgt
      721 tcaccgcgcg agagcgccca gactatttgt atgcgcgagg aatcacccat ggcctgaaac
      781 gtttcggcta agagcaagaa aacccgctgc gagcgcattt tggccagctc tttggattga
      841 atgttctgta aacgcataag catgcgttga tgtaacaggg tcgtcggggt tttgcaaacg
      901 gggtggggaa tggggggtag ggcacctcgg aattaaagat ttgctgacaa gtgttgaata
      961 attagacacc tttgggctga ggtaggttgt aatgaattca aatagttatt ccgtgctctg
     1021 ctcatcaata aaaaccggca tcggtcttgc tcatggcagg tgcgccatga ttcgatatgg
     1081 aatttttgca ttagtatttt ggaccttttt aactggcact gcacgggcta gccttgaccc
     1141 cctacccttc ctggcagtgg atgccgaaac cgcccaagca atcgaagccg atattgatcg
     1201 gatgctttta gagcaaaaag aaaatctcga catcacttac atgaccaaaa aatggcttag
     1261 taaagatgat cctacgatga cgcaagaaag gttcgaggca gcggtggctt ggattgctaa
     1321 cgatccttac ggccgtcaag tagtgatcga tgtgatgaag cgcgcacgcg taaaagaagt
     1381 ggccgggatt atgccctacg taactgtgca agaactgcag gcacgccaat ggatgattga
     1441 tgcctcagac gaaaatcgaa ccattgaagc aatgggccgg actcaatatt catatacggt
     1501 acccacaagt cttacgccgg atctggaacg cttcatggca attgagagtt tgcggctcgc
     1561 ttcggagccc aaccttacaa tggaagagca ggaacaactc tatcaaccgc tgctgttgga
     1621 aatgatcgag aaactaacga aatctgacgg attaattaga gatttatttt tcgcacgtac
     1681 aaatacagat ctttcgttag tatacggcat ggaagtaccg cgcattctag tgaagcccaa
     1741 tgtttcgatg cgaacggcgg tcatagcttt cgcccacgaa ttcgttcatt ttgcctatgc
     1801 tacatttcga gaaagattaa ctttaattga ctacgacaac gaggaagatg ctgcttttgc
     1861 atttgttgat ggtcagggcg aagaagttga tgctcatatt tttgaatttg gcttgaactc
     1921 gcgtatgtat aagaatcctt acgcaattca aaaagaaatt cgggatctat tcgactcaga
     1981 tgcggttttc gttggagatc gtcgaaaatt cgcaaaattt attcttgaag aaaaaagtta
     2041 tcaaaaaagc ttcgaacgtg aatattttaa cgccttactg acgagctata agttcgaata
     2101 caaggctttg aaaaaactcg agggcctcta tttgattcgc gaaactgaag aagagcaata
     2161 tcagaaaaaa ggcaacgacc gaaaagccaa tgcggccctc gcgtcgaagg ttagaattcg
     2221 aaaaacaatt gaacagtacg aagaaaggct aaagaagtat gaagataaac ttggcatcgt
     2281 ccgcaagaaa aagtcggcct aaattttttc tcagctggga agacgcactt tggctcggcg
     2341 ctgtgcttgt cacgatcgtg gcaggcgcta cgattcacta aagatcactg aatgaagtag
     2401 ggttgagtcc acgctcgagc gccatttaga gtgattcggg ctcgtacgta accctcgctg
     2461 ccattgatct gataaactgc cggcatggtt cgggtcgtgt tcaaagtgcg gcccgattct
     2521 cctagaaact caacagtacc actagagttc caattagata ctacgagtgt gaaagtagga
     2581 cccttcacat tcaacctcgt aatgaccggg ccacgggaaa agtagaaatc gccacgttgg
     2641 agtgccgagc aaacctctgt agcagtcaac ctatgggcag ccacctggac ccaggcccga
     2701 ccgggattgg ctttagtggc aatatcaaaa tcgctgtagt ggtgtgaatc gtctacccct
     2761 actccccata cagagccgcg gtgggtgaga tagtcgtccc aaagcgcttc gtgagatatt
     2821 cctttttctc caccgttttc gttgacgagg gggtgcccac tggtgacttc cagaaaatca
     2881 aagtcgccga ctttacgcag aatttttgca ccaaatgccc agccaaagtt agggtgatta
     2941 acgatcgtca acgcgccgtc ggctcgggcg gtattgattt ggtcttgcag tatggctccg
     3001 ggctggcgat tttcccgaat ccctttgagt tgagattttc cacagatagc atttacatga
     3061 atgggaactc gataagagcg accggtgatt tcgtcgccgg gaagtgtaat aaatttcgaa
     3121 gtggaatgag agctggtgtc gacaagtttg tcgtgttcag tcatcgataa aaagtcgtaa
     3181 ccgttgcgtt tataccactc gacaaccgta ttgagtggag cgtcgccatc actttcttgg
     3241 ctgtgggtgt gtaggttgcc tttgaaaaaa tgttttagtt ctacggaccg gtcgaattta
     3301 gtaccgttaa tcgcgaggtg cgtaccgcgc ttggcggggg agagcgaaga acaagaaatt
     3361 agtgagcaca tacccgaaag gaagattacg gacctcaacc accgattaag cgaaaaactt
     3421 gtcaacatca tggcgtgcgg tataccccaa atataggtga tttatgaaag acttcttttt
     3481 tcaaataata cgttctgacg aggggtgtag atcctacgtt ttggccgata gcggcactaa
     3541 acgtgggctt gtttgggacc catcactagc tgggctcgaa agcaccctcg aagttgtcgc
     3601 ggaacagggg ctaacgcatt taattctcgc ggattcccac actcatgcgg accatttttc
     3661 cgcaaccgct gagctcgcca aacggcttcg agatgtagct aaggtggaaa tcgtaatggg
     3721 cgagaaaacg gagtctgaac ggcccgggcg tcgtctgcgt gatggtgacc gactagacat
     3781 gggtccaaaa atgcaggcgg tagtgctggc tacgccagga cataccgaag acagtatttg
     3841 tttatacatt gaggttggcg gagaaaagtt agttttaaca ggcgacagct tacttattgg
     3901 ttccagtgga cgcaccgatt ttccgggggc ttctgccgaa gcgctttatc attcgttgca
     3961 cgaaaaaata atgaaattgc ccggaactac ttggttgttt cccgggcacg attattcagg
     4021 atatctcttt agtcgcctgg aagttgaggc aaaacaaaat tcccaactcg ccatggataa
     4081 aagcgaattt cttttgttta aacagaacga agtgttggac tgtacgcctg tagtgccgaa
     4141 gatagtcgat ttcaatcggg catcttcgcc cagtgcgccg atgcatattc ctgcagccgc
     4201 tatttgtgcg cgatcggaac cggtgccagg cgtaaggtca attggaaccg acgaattgcg
     4261 ttctttgatc ggtgagcact caaagaaaag tctctttatc gacgtgcgcg agcccgacga
     4321 ggtcaataaa gctagaatcc ctggactaca gaacattccg ctttcattag tatggaccaa
     4381 gttaccagag attgcggcta gtgagagtgc ttacttttcc tgccaaagtg gtagacggag
     4441 tggaatggta acggcaacgc ttgctcgttt aggtttcagc aacgctataa atgtagcagg
     4501 cggaattcaa ggttggatgg cgcgcggtta tcacgtcgag gcgtcttaat tgaagttagg
     4561 ttcttcgggg ccggccccat tttgagaggg agttccgaag tcgacggagt ctgctcccgt
     4621 ggcttcgccg acactggcgc cggcttcatt gacccgcgtg gtggtcgtag tggtgctgct
     4681 tggcgcgcga gatttggtag gtactgaaat catttttcca ccgattacgt cttcaatgta
     4741 tgcgcgttcg gtcggctcct ttgatttcgc tccgcccgag agttcatttc ttttcatttt
     4801 ttgaaaattt tccgcgcggt cagtcatgtt cttttgaagt ttatctgcaa gaagactgta
     4861 ctttttcacc acttcattat tcgcttcggt gcgagcttta aaatacgggg ccatggattc
     4921 cttgcgaacc cgttctcgct cagattgagg gggattaatc cctaaggcct gggttttttc
     4981 gtacatgtcg atgtaggctt ttccgagtac acttaatgtc gaagattttt tttcgccgcg
     5041 agcgtggacc tggcgtagta gacccgtcga aagtaatccc gtcaggacca agccaatcac
     5101 gaaaaacgca cgatcgcctc gcgatttgta atgagttaaa tggatattca gaagaccccc
     5161 ataatacccg atcaaattaa ttgtatacat acctgactaa ttttgctaga atttttattg
     5221 tgtgaggggg ataggcaaaa tgcctaataa tattagattg ttatttgggg tcatgggcct
     5281 ctttttgagc accattgtgc ccgcccaaga gattcaaaac gcactcgata atggtccgtc
     5341 tcgtgaggat cagctagatc gcgctgccgg aatgcccact aaatctgagt tggattatcg
     5401 cacgctcact gaagccaagt tgagttgccg caacaaaggt tcacagagct tcttgggttc
     5461 aaaaaagtgt gggaatgtcg gctcggaact tggcttgaag ggcgaaatac agaagcgctt
     5521 taaagactcc atgaaggcct atgctaaaaa ttattacaaa ggtgtcattt atacggattt
     5581 aagtgcgccc gacggcgcgc cccgaaacat tgaagttccg gtgtgtaatc tgtcggtcgg
     5641 tgaaggtgaa ggggctgcaa atcagaacta taagggtgac tcctgtttgg accttgcgcg
     5701 cctgacagtc gatacgagct acacttcggc ctccgcgaaa gttactgcaa atctattgaa
     5761 tgggcgggtt aaagaattcg cctggtggcg tgggctttat cttcaagttt actttaactc
     5821 cgctggcaaa gtgatgcggg agttcgaaga acaaggcaaa gtaaccttaa cgcgcttgcc
     5881 ggacggaagt gttccgaccg gaccgattgc tcgggacgct ctaaggttgc aaataggccg
     5941 cgatcgggtg attcgagaaa ttgcttcgat caaagggagc gatcaagaag agttgggtta
     6001 cgctcgcgtg gcagaaacca aatgtgaagc gcgtgacgat aaaataactt ctcacgcctg
     6061 tagattgtcg atggtcaatg aacacgaagt cgcgcagtgg acatatctca tgatggcaga
     6121 agcgaacatt cggactcagg tggaagtaga ccgccagttc gaaaatatgg aaactctttt
     6181 agaggggttt cagttacggc tagagccaat ttgcaagaac cagtatgacg ccgactgcac
     6241 cttgtgtcgc gacagcacga gaacacgaag aaccaatcgc tgttatcaag aacaaatgcc
     6301 taatatgatg aagcaggaat tggctcggta tcctaccgac aatttacttc caattcccta
     6361 cgaagagcta aaacgagaag tcggtgatca agcgagttct tcgaaaaaag acgccggttc
     6421 gtctagtcca tttcaatttg gagacgttag caacctatta gccttaagtt tgcttggctt
     6481 tcgtt
//
LOCUS       DAOMXO010000038         6479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6296_length_6479_cov_5.372198, whole genome shotgun sequence.
ACCESSION   DAOMXO010000038 DAOMXO010000000
VERSION     DAOMXO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6296_length_6479_cov_5.372198"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLH57_01510"
     CDS             complement(<1..285)
                     /locus_tag="PLH57_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01316.1"
                     /translation="MICRLSMRYILVFSLLALIVGAACTPRRDIGNTLRVAQREKIKT
                     LDPAHANDFFSGTAVRRTYEGLYQYHYLKRPYEVVPNIAAAMPAISADGKT"
     gene            complement(353..1105)
                     /locus_tag="PLH57_01515"
     CDS             complement(353..1105)
                     /locus_tag="PLH57_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01317.1"
                     /translation="MIKVLLLGFCSLLVTACAHRSAEFSPRKAVESIAATSAAVKTVR
                     GKAWISVKTPEGAVAFPANVAIDRTRAGHSLLRLEAVDLVGTTHAMMLLNETGRFLWV
                     DFDRRSYFEVTKTWYGLPVREFPGLLLGLPQLPSDGRVSAHGDLGFQVSTGGSAASSG
                     NVLIEMQWVDPGPRLFAKSVSSPGLGYSVEYSAYQDTKDFYLPAKVGLSGKNIQIQLD
                     WREQIWNEPIDATVFSSAALERALKKFRKVRQ"
     gene            complement(1147..3003)
                     /locus_tag="PLH57_01520"
     CDS             complement(1147..3003)
                     /locus_tag="PLH57_01520"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK01318.1"
                     /translation="MVSGSKNKKFFAVVATLWSVVIAALLAACSTVSRSPNITEIDPA
                     TSSGIDSPSPEKLENESGAKTTSFPSAHIDPSLSGNQAATHFALGQAYSLEGNSEKAI
                     EEFKLALVYDPKSAVVHTRLAGEYVKRGLLTFAIEECKAALELDRTHVDARLLLAGLY
                     SSTKLIKEALEQYDLVLKHDKNNTEAYVFKGSLLLEEGRAKEAITVLRKLVDKDPESF
                     YGYYYLGRAYQHEEKDTQAIQYYRKALEIRPNFSQAALTLGVLLEEKKREKEAIAVYL
                     DLYEQAGDLQAVARLAQLHIDREDYDQALKFLLLLDQADDENLNVRVKIGLIYVERKK
                     YDQAIEVFKSILEKNPEAEKVRYYLASVYEEHKREDDALREFKYLAEASESESSLVVD
                     SSLHVAQLLKGKGDHEGALRWMDRRIARAPKILQYHLFKASLLEDGKQIDAAVKALEN
                     TRSEFGDDERVLYYLGSLYDKQGRQNDAIELMQKILAINPNNVHALNYVGYTYATRGQ
                     KLDEAERLVFTALKLKPNDGYIVDSYAYILMVRGKLPQAIKEFERAHKLRPDEGIIVE
                     HLGDAYFKHNLKRKALQKYRDAVKLYTDPTDQEKVLQKVRSIESSSRLPAQE"
     gene            3344..3685
                     /locus_tag="PLH57_01525"
     CDS             3344..3685
                     /locus_tag="PLH57_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRK01319.1"
                     /translation="MKEVKVIKRYQNRKLYDTNQSCYVTLDEIAQMIKNGDDLRVIDN
                     KSKLDITAVTLTQLLYESERKAKNAVPVEMLKEIIRGGDGSFSGYIRSNLSTQLRKFD
                     SDTAESTTLSQ"
     gene            3719..4342
                     /locus_tag="PLH57_01530"
     CDS             3719..4342
                     /locus_tag="PLH57_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01320.1"
                     /translation="MKLFIEKNLRSLYVVLALVFVSFVSQSALAQQGGGSGPAIGSHT
                     AGFGLGQVMLLGDFGQRLDDSIGFQFLYEYQASELFGLYVTIEKSSHSNANETEKLSL
                     FGVIPHLRANMAYVDQLTLFGFTGFGLMKASQTQNNVEGSVLTVVFDVGGGFDLALSE
                     RFVFGTTFAFHNVFGKTDSVTITTNDPDGFSIGGTYLSLMLNVKYRF"
     gene            4372..5373
                     /gene="ribD"
                     /locus_tag="PLH57_01535"
     CDS             4372..5373
                     /gene="ribD"
                     /locus_tag="PLH57_01535"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRK01321.1"
                     /translation="MNVPAHSRFATDLEAMALAVEQAYQGFGRTAPNPPVGACLIAND
                     GRVLGLGSHRGAGQAHAERTAIDQAQQLFGTNAARGATLYVTLEPCNHTGRTPPCTEA
                     IAQAGIARVVFANKDPNPTVPGKGAEALAARGIQMIHQPNDAAERLLRPFRQWITRRT
                     PWVVHKIAFRRTHENKLSMIPESQNTFARAESLRLAHELRRQSEAILTSVPTILADLP
                     KFTVRHTWDHDDLSFKRKIMIVSSRATGLTVETLHTVLPVTWLSETRAAGFEVLLSPN
                     LEHALSTLGQLNVHQVLVEAGPRLSEHLASTRTAHENVVISVGEERDFVLRTLANKS"
     gene            5384..6004
                     /locus_tag="PLH57_01540"
     CDS             5384..6004
                     /locus_tag="PLH57_01540"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRK01322.1"
                     /translation="MFTGIITATGRVTKTETARADGSHTLERTLTLATPYADAVLGES
                     IAVNGVCLTVVQKVDSSRGTELCFFVSSETCDRSNLGSLSEGDEVNLERALRATDRLS
                     GHIVQGHVDGLARVTRIERQNESVLLGVSIPAVLQKYLVEKGSITLDGISLTINALAE
                     GEAVLTIIPHTWNHTTLKTKKVGRDLNLEVDVIAKHVEKLTNAYHR"
     gene            5988..>6479
                     /gene="ribB"
                     /locus_tag="PLH57_01545"
     CDS             5988..>6479
                     /gene="ribB"
                     /locus_tag="PLH57_01545"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRK01323.1"
                     /translation="MHTIAEAIEAVRTGQFIILVDDEDRENEGDLVIAAEHITPDAIN
                     FMAKHARGLICLALAPEIVDQLELPLMTRRNHSPYGTAFTVSIEARAGVSTGISAADR
                     AHTIKVAAHADARPDDLIAPGHVFPLRAQKGGVLVRAGQTEGSVDLARLAGLRTAAVI
                     CEIM"
BASE COUNT         1575 a         1681 c         1584 g         1639 t
ORIGIN      
        1 ggtttttcca tctgctgaaa tcgcgggcat cgccgcggca atgttgggca ctacttcata
       61 tggacgcttc aaatagtggt actgatacaa gccctcataa gtacgtcgaa ccgcggttcc
      121 agagaaaaaa tcattcgcgt gcgcggggtc caaagtttta attttctcgc gctgtgcgac
      181 cctcaaggta ttgccaatat cgcggcgcgg agtgcacgct gcaccaacga tcaacgccag
      241 taacgaaaaa accaaaatat aacgcatcga taaacggcaa atcaaagctt gaatcctcta
      301 gcaaaaaggt gcccgcatca attcgggttg tcaaacaatg cgccgcgagt ttttactgac
      361 gtacttttcg gaattttttg agcgcacgct ctagcgcagc cgacgaaaaa accgtcgcgt
      421 caattggttc gttccaaatt tgttcgcgcc aatcgagttg aatttgaata tttttaccgc
      481 tcaaccccac ttttgcgggc aaatagaaat ctttggtatc ttgataggcc gaatattcaa
      541 ccgaataacc gagccccgga gaagaaacgg acttcgcgaa aagtcgtggc cccgggtcaa
      601 cccactgcat ctcgatcaga acatttccag aactcgcggc cgatccaccg gtactgacct
      661 gaaatccaag gtcgccatgc gcacttacac ggccgtcaga aggcaactgg ggtaggccca
      721 acaatagccc gggaaactct cgaactggaa gtccgtacca ggtttttgtc acttcaaaat
      781 aggaccttcg atcaaagtcg acccacaaaa agcggcccgt ttcgttgagc aacatcatgg
      841 catgagtggt ccctaccaag tcgacagctt ccaaacgcaa cagactatgc ccagctctgg
      901 tgcggtcaat ggcgacattg gccggaaacg cgactgcgcc ctcaggggtt tttacgctta
      961 tccatgcctt gccacgtacc gtctttaccg ccgccgaagt agcggcgatc gactccaccg
     1021 ccttgcgggg actaaattcc gcggaacggt gagcgcaagc tgttaccaat agcgaacaga
     1081 atcccaacag tagtactttt atcatgtatt caaaaccaaa tgtggcggtt tacggattga
     1141 agaggttcac tcttgcgcgg gcaacctaga actagactca atgctccgca ctttctgcaa
     1201 gaccttttct tgatccgttg ggtcggtgta gagctttaca gcgtcgcgat atttttgcaa
     1261 cgctttccgt ttgagattgt gtttgaaata ggcgtcgccc agatgctcta cgattattcc
     1321 ttcatcaggg cgcaatttat gggctctttc aaattcctta atcgcctggg gtaactttcc
     1381 ccttaccatt agaatgtaag cgtaactatc tacaatgtat ccgtcgttag gtttaagttt
     1441 taacgcggtg aaaaccaatc gttccgcctc gtcgagtttt tgaccgcgag tcgcgtaggt
     1501 gtatcccacg tagttgaggg cgtgcacatt attaggattg atcgctaaaa ttttctgcat
     1561 gagttcgatg gcgtcgtttt ggcgcccttg cttgtcgtag agtgagccga ggtaatacaa
     1621 cacgcgctcg tcatcgccga actccgatct tgtgttttca agcgccttga ctgcagcgtc
     1681 gatctgtttg ccgtcttcca gtaaggatgc tttgaagaga tggtattgta ggattttcgg
     1741 tgcgcgcgca atgcgccggt ccatccatcg caaagcccct tcatggtcgc ccttgccctt
     1801 tagcaattgg gctacatgta gcgaagaatc cacgaccaaa ctgctttcag actctgaagc
     1861 ttcggccagg tacttgaact ctctcaatgc gtcatcttcg cgcttgtgct cttcgtacac
     1921 cgaggccaga taatatcgaa ccttttcggc ttcaggattt ttttcgagaa ttgacttaaa
     1981 cacttcgatt gcttgatcgt atttcttgcg ttcgacgtag attaatccga ttttcactct
     2041 tacatttaaa ttttcgtcgt cagcctgatc gagaagtaat aaaaatttta gcgcttggtc
     2101 gtaatcttcc cggtctatgt gcaactgcgc caggcgagcg acagcttgta gatcccccgc
     2161 ctgctcgtac aggtccagat acacagcgat agcttctttc tcgcgttttt tttcttctaa
     2221 caaaacgccg agagttaatg cggcctgcga aaaatttggg cgaatctcaa gagctttgcg
     2281 atagtactga attgcctgcg tatctttttc ttcatgctga taggcgcggc ccaagtagta
     2341 gtacccgtaa aaggactccg gatccttatc gaccaatttt cgcaacacag tgatggcttc
     2401 tttggcccga ccttcttcga gtaagagcga acccttgaaa acgtatgctt cagtgttgtt
     2461 cttgtcgtgc tttagaacta ggtcgtactg ttcgagcgct tcttttatga gtttggtcga
     2521 cgaatataat ccagcgagca ataaccgcgc gtctacatgg gtgcgatcaa gctcgagtgc
     2581 ggccttgcac tcttcgatcg cgaacgtcaa taaaccccgt tttacatact cccccgcgag
     2641 tcgagtgtga accactgccg atttcgggtc ataaaccaag gctaacttaa attcttcaat
     2701 cgctttttca ctgttcccct caagcgaata agcttggccc aacgcaaaat gagtggccgc
     2761 ttgattgccg ctcagcgatg ggtcgatgtg ggcagaagga aaagatgttg tttttgcacc
     2821 agactcgttc tcgagttttt cgggactggg ggaatcaata ccggaacttg ttgccgggtc
     2881 aatttcggtg atgttcggcg aacgcgaaac ggtggagcaa gcggctaaaa gcgccgcaat
     2941 tacaacgctc cacagcgtcg cgactactgc aaaaaacttc ttatttttcg agccgctcac
     3001 catgacattc ctatctgttt cagacccttc gccgccctgc gccctattca gttgacaacg
     3061 tttggcgtca atgaatataa gttttcacta gggggttgat gcatctagcc ttccccattt
     3121 gctagatgtt tcgacaggtt tttatctcca ctttagtaac ggtcgccgtt ttacgttcgc
     3181 cgttggggcg aactctggtc gacgatttat tttgaatcgc gaacccggca aaaacgtccg
     3241 tccggcacgt gattttgtgg ctagtgtggt gccctggggg tagttcgtag ttaattaaga
     3301 gcgaaaaatg ggaactcagt ttgtttgagg gggagtatca atcatgaaag aagttaaagt
     3361 cattaaacgt taccaaaatc gtaagttgta cgacacgaat cagagctgtt atgtgacgct
     3421 cgacgaaatc gcacaaatga tcaaaaacgg cgacgatctg cgcgtcatcg ataacaaaag
     3481 taaactagat atcactgcgg tgactctgac ccagcttttg tatgaatcgg aaagaaaagc
     3541 aaaaaatgca gttccggttg aaatgctcaa agaaatcatt cgcggtggcg atggttcttt
     3601 tagtgggtac attaggagca atctgtcgac tcagttgaga aaattcgaca gtgatacggc
     3661 agaaagcacg acgctcagcc aataatataa aaaagattca gaaccaaagt cagacaacgt
     3721 gaaactcttc atagaaaaaa atttgcggtc gctttacgta gtactggctt tggtttttgt
     3781 atcttttgtt tctcaaagcg cgcttgccca gcagggcggc ggctcggggc cagccattgg
     3841 ctctcacact gctggctttg gtttaggcca agtcatgttg cttggcgact tcggacagcg
     3901 tcttgatgac tcgattggtt ttcagttctt gtacgaatat caggcaagcg agctatttgg
     3961 actctacgtc accatcgaaa agagcagcca tagcaacgcg aatgaaacag aaaaactttc
     4021 actttttggt gtgattcccc atttgcgggc caacatggct tacgttgatc aactcacact
     4081 ttttggcttt accggttttg gtcttatgaa ggcgagccaa actcagaaca acgtcgaagg
     4141 ctccgttttg actgtggtat tcgacgtagg gggcggattc gatttggctc tttccgaacg
     4201 cttcgtcttc ggaacgacgt ttgcctttca caacgtattt ggaaaaaccg acagcgttac
     4261 gattaccacc aatgatccag atggatttag catcggtggc acctaccttt cgctgatgct
     4321 caacgtaaaa tatcgatttt aaatagaaga tcgtccgagt acagtcgcgc catgaatgta
     4381 ccggcacatt ctcgatttgc cacagatctc gaggccatgg ctctggcggt ggaacaagcc
     4441 taccaggggt ttgggcgcac cgctccgaac ccgcctgtag gcgcgtgctt aattgctaac
     4501 gacggccgtg tgcttgggct gggcagccac cgtggcgctg ggcaagccca tgccgaacga
     4561 acggccattg atcaagcgca gcaactcttc ggtaccaacg ccgcccgcgg tgccacactc
     4621 tatgtaacgc tcgaaccctg taaccacacc ggccgcaccc ccccctgcac cgaggccatt
     4681 gcccaagctg gaattgcgcg cgtggttttc gcaaataagg accccaaccc cactgttccg
     4741 ggcaaaggcg ccgaggccct ggccgcccgt ggcattcaaa tgatccacca gccaaacgac
     4801 gctgcagaac gtttacttcg cccctttcgc cagtggatta ctcggcgaac tccgtgggtg
     4861 gtgcataaaa ttgcctttcg ccgcactcac gaaaacaaac tctctatgat tccagaatcg
     4921 caaaacacat ttgctcgcgc tgaaagcttg cgactcgctc acgaactgcg acgtcaatcc
     4981 gaagcaatac tcaccagcgt acccactatt ctggccgatt tgccaaagtt tacagtgcgt
     5041 cacacttggg accacgacga tctctcgttt aagcgtaaga ttatgattgt ctcttcgcga
     5101 gctaccgggc tcacagtcga aacgctgcac accgtgctcc ctgtcacatg gctctcggaa
     5161 acacgtgctg cagggtttga ggtgctctta tcacccaatt tagagcacgc tctttctact
     5221 cttggacagt taaacgtgca ccaggtgttg gtagaggccg ggccacgcct cagcgagcac
     5281 ctagcctcaa cccgtaccgc gcatgaaaac gtcgtcatat cggtaggcga ggaacgtgat
     5341 tttgtgctaa gaacgttggc gaacaaatcc taggagtttt tgggtgttta caggaatcat
     5401 cacggccacc ggccgagtca ccaaaactga gaccgcacgc gcagacggta gccacacgtt
     5461 ggaacgcaca ctcacgctgg caactccata cgccgacgcg gttctgggcg aaagcatcgc
     5521 ggtgaacggc gtatgtctta cagtggtgca aaaagttgac tcctcccgag gtactgagct
     5581 ttgttttttt gttagctccg aaacttgcga tcgctcgaac ttggggtcct tgtcggaagg
     5641 cgatgaagtc aatcttgaac gcgcgttacg cgcaactgat cgcctgtcag gccacattgt
     5701 tcagggccac gtcgatggac ttgctcgagt cacacgaatt gaacgccaga atgagtcggt
     5761 attactcggc gtttcaatac ccgctgtttt acaaaagtat ctggtcgaga aaggttcgat
     5821 tacgctcgat ggcattagcc ttacgatcaa cgcccttgcc gagggtgagg cggttttaac
     5881 tattattccg catacttgga atcacaccac cctaaaaaca aagaaggttg gtcgggattt
     5941 aaatctagag gtggatgtga tagcaaagca cgtggagaaa ctcacaaatg cataccatcg
     6001 ctgaagcgat cgaagccgtt cgtacgggtc agtttattat tcttgtcgat gacgaagacc
     6061 gtgaaaacga aggcgatctg gtaatcgcag ccgaacatat tactcccgac gccataaact
     6121 tcatggccaa acacgcacgc ggacttattt gtttggcgtt ggctccagaa atcgtcgacc
     6181 aactcgaact cccgctcatg acccgtcgga accattcacc ctacggaacc gcgttcactg
     6241 tatctatcga ggctcgcgct ggcgtgagca caggaatttc tgcggcggat cgtgcgcaca
     6301 ctataaaagt ggcggcgcat gccgatgcgc gcccagacga tttaattgct ccgggtcatg
     6361 tttttccatt acgcgctcaa aaaggcggcg tgctcgttcg tgccggccaa accgagggta
     6421 gcgtggatct agcacgactg gctggcctgc gaacggccgc tgtgatctgc gaaatcatg
//
LOCUS       DAOMXO010000039         6471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6305_length_6471_cov_4.366272, whole genome shotgun sequence.
ACCESSION   DAOMXO010000039 DAOMXO010000000
VERSION     DAOMXO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6305_length_6471_cov_4.366272"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1669
                     /locus_tag="PLH57_01550"
     CDS             146..1669
                     /locus_tag="PLH57_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK01324.1"
                     /translation="MYSAVLTCTLVGFEAHAVVCEVHLANQLPSVTLVGLPSSITQES
                     RDRVRSAIVNSGLEWPARKVTINLSPAMLPKWGSHFELAMALGILLAGRVPKKVFAVG
                     ELSLSGQVKACGWSGLILPVLRDVRTVVAHPVDIAEIQNQMSANDVAKLRWISAENLN
                     QAIEAFSAAPAVDLSDADLSVDPPAVQVTSPDYALLKQVTGEPLAVLAALCALKERFH
                     LLLAGSHGVGKSMLARALTLALPPLSETELQSRKTLSAMFGELPFKNNGRARPVISLQ
                     SGATKQSLEGSLLDSGRVLPGELTRAHCGVLIVDEFLELRRDVIESLRQPIEEKCVRM
                     SRAKLKTVLPADFQLIATTNLCPCGYLGHARRRCRCGGPSAVRYLTKLSGPMLDRFDA
                     IILMGDPRVESELSGPARDLLGWLTNPVHWPGLFERGPDARSLRNEPAFLWQDLGEAD
                     PGWSPRVREKQLRLARVIATLAGEHRGLPPTVYFELANALRADLDWEVRRDGAIARV"
     gene            1739..2485
                     /locus_tag="PLH57_01555"
     CDS             1739..2485
                     /locus_tag="PLH57_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01325.1"
                     /translation="MNLRFVARVLVVLTLACSFAACGKEKKADKGYMKECVLPADQTN
                     AFQGAWRQRPIKIAFRTGHWDDAQIGAIVAAANTWNEFFKSSKGGPIFDTGGGAYRST
                     ANLTAPNCSSTDGVVVYKRQAAGSWPAELGPSAAGATGVVSCGFGNGANGYPILAAGM
                     MEFNFVNYFREGTTALLDLQTVAVHELGHLLGLDHACGPIKNGKPNASCPKYLSAVMY
                     PSAFYDSATRVGQIKRNLDTNDQGRANCLY"
     gene            complement(2508..3968)
                     /locus_tag="PLH57_01560"
     CDS             complement(2508..3968)
                     /locus_tag="PLH57_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRK01326.1"
                     /translation="MARPAHRPESFRAGQIYDSIYGFIRLTKTEEEIINSAFFQRLRW
                     IKQLGFASYIFDGAEHTRFAHAVGVLHCADQMVRAIGRGVPDAQLYSTKTTDANSMFH
                     KSIRIAAMLHDIGTFPFSHSIEGSYIKYGESLRQRGELPGKQLPNNHEHLGSYLIKNT
                     DFKGGITRILKESGLDFTSLSKFIKGDSGNLVANQILHSDIDADRLDYLVRDAHHTGI
                     KYGHIDKDYILYHLTTAFTDGKKEILAVRENAVHAVEDFLIARFAWYSQVIRNSSSAK
                     FDILAAHIASYLLKNNLIYHYHELLELAAKDPERFFGFNDVYFMSRIQDLYWSKRIPD
                     PIVNEQMRMLIYRVPPKTIRLKESEHRILETGTGKNSPREEAVKKLNAKIQEIQHLIT
                     KHGKGREWIIADVPSKDVIFTKDYASLMGTRKDRSQLYTERDPVKIVSKHGKISLLVE
                     RENTLVSKLSRSINFIPNVYGNDAAYALLKARKIVE"
     gene            4074..5285
                     /locus_tag="PLH57_01565"
     CDS             4074..5285
                     /locus_tag="PLH57_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01327.1"
                     /translation="MFLKFNSTSSSVLSLGVLAALVSILSGCGRELKIRVAPSEEVRH
                     SAASDLVRQVEIRYRNAAGEERFRESVSVTGLGQPLDIKNESIQDLDWSKDGRWEANA
                     FNGDGALMLHASTQADPEEDEQIDLVLARASGPQAPANLNSVFLKSFRTTGFSVDTKH
                     SVEKLRSPVCYELPAIYLDAGSGALARGVFFETSVAGTAVRVAMSKDGERRVLPVHSS
                     GDSYLDLADEIEKIGSTRRRNLYIFTDEYEGCVTLSAGLSNLLDPTLTYKFLYEAGRN
                     VGMGKGMAGEIVVLNPNPIEVAVAVNCSIYSNAQDPNGIFIGPLGRDHYVYKEECQRT
                     NANHSFTRSGVIAKRDTFRVPVCGYGNLYGESQGHSGLVHTGFTLQCSARLEVDGLIG
                     SSPTVFLTAPK"
     gene            5301..5771
                     /gene="rimI"
                     /locus_tag="PLH57_01570"
     CDS             5301..5771
                     /gene="rimI"
                     /locus_tag="PLH57_01570"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRK01328.1"
                     /translation="MESNFSIRPATEDDLPAILEIENSVYPIPWTAEQFKAEIEKPFA
                     NVLVLTDDDTDQIVAGYVVFWMMFDECHILNVAVHPNWRGLGIGGKLTRIAVDFALKK
                     ELKRVFLEVRKSNEAAVKLYQKVGFFIDHIKPRFYDNGEDAYFMVMYLTRPNHF"
     gene            5891..>6471
                     /locus_tag="PLH57_01575"
     CDS             5891..>6471
                     /locus_tag="PLH57_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRK01329.1"
                     /translation="MYRALKNGLILSSFFAVVFVLKPADQAAERAWLASLSDPKEALL
                     AKQHSKRSKKAFGAKEVRRFLERQIKQASPEQLDSITQVILEVSKSYHFHPALILSLI
                     RVESRFNAWAISPKGAIGLMQLMPETGRWLADRMGARWDGPAMLLDPEINIRFGTYYL
                     AYLRERYNGDPKFYLSAYNVGPGRMDGWATTGY"
BASE COUNT         1548 a         1512 c         1711 g         1700 t
ORIGIN      
        1 aaaaacactc ggtatttcat agattccatc ggatttcgga aaataaaact taatagcacg
       61 aaattaataa ggaaaatcta tagtttacga aattattccg gaaaattttg ccgctttggc
      121 atactcggtg aacctgccgc agaacatgta ttcagctgtc ttaacgtgta ctttagtcgg
      181 gtttgaagcg catgccgtag tgtgtgaggt gcatctagcc aatcagttac caagcgtcac
      241 cttggtcggg ctgccgagct caatcaccca ggaaagtcgc gatcgggtgc gatcggctat
      301 cgtaaacagt gggctcgagt ggccggcccg aaaggttaca attaatcttt cgcccgcgat
      361 gttaccgaag tggggcagtc actttgaact cgcaatggca ctcggaatat tactcgcagg
      421 gcgagtgccg aaaaaagtat tcgcggtggg cgaactctca ctcagtgggc aggtcaaagc
      481 ctgtggttgg tcgggtttga tcttgcccgt gttgcgggat gttcgaactg tagttgctca
      541 ccccgtagat attgcggaaa ttcagaatca aatgtcggca aacgatgtgg cgaagttacg
      601 ttggatttcg gccgagaatt tgaatcaagc gatcgaagct ttttcggctg ccccggcagt
      661 tgatttatct gacgctgatc tttcggttga tcccccggca gtccaagtga cctcgcctga
      721 ttatgcacta ttaaagcagg tcacaggcga gcccctcgcg gttctcgccg cactttgtgc
      781 gcttaaagaa cgatttcatt tgttgttggc tggctcccac ggagttggaa aatcaatgct
      841 tgcgcgcgcg ttgacactgg cactccctcc gctctcagag accgaactgc aatccagaaa
      901 aactctttcc gcaatgtttg gtgaactgcc atttaagaac aacgggcgtg cgcgcccggt
      961 gattagttta cagtcaggcg ctaccaaaca gtcactcgaa ggttcgctac tcgattccgg
     1021 gcgggtgctc ccgggggaac tcacacgtgc tcattgcggt gttttgattg tcgatgagtt
     1081 tttggaattg cggcgagatg tgatcgagtc gctgcgccaa cccattgaag aaaagtgcgt
     1141 gcgcatgagt cgcgcgaaat taaagactgt gttacctgcg gattttcaat tgattgcgac
     1201 aacgaatctt tgcccatgcg gatacctcgg gcacgcaagg aggcgttgcc ggtgcggcgg
     1261 tcccagcgct gtgcggtacc tcaccaaact ttctgggccc atgctcgacc gattcgacgc
     1321 gatcatttta atgggtgacc cccgcgtcga atctgaattg agcgggcccg cccgtgattt
     1381 acttgggtgg cttacaaacc ctgttcattg gcctggcctg ttcgagcggg gccctgatgc
     1441 gcggtcgctc cggaacgaac cggcttttct gtggcaagat ctgggcgagg cagaccctgg
     1501 ttggtcgccg cgggtgcgcg aaaaacaact tcgcctcgcg cgtgtgattg ccacgctggc
     1561 tggcgagcat cgggggcttc caccaactgt gtacttcgaa ctagcaaacg ccctacgtgc
     1621 cgatctcgac tgggaggtac ggcgcgacgg ggccatcgct cgtgtataga gcaataattg
     1681 ccgattaaag actgaccaaa agtattccga actcattagt atgttaatgg atggttcctt
     1741 gaatctgcgc tttgtcgcgc gagttctagt ggtattgact ctcgcgtgta gcttcgcagc
     1801 gtgcggcaaa gaaaaaaaag ctgacaaggg ctatatgaag gaatgcgtat tgccggccga
     1861 ccagacaaac gcatttcaag gtgcgtggag acagcgtccc attaaaattg cctttcgcac
     1921 cggtcactgg gatgacgcac aaattggtgc gatcgtagca gctgctaaca cctggaatga
     1981 gttttttaag tcttcaaagg gtggtcccat tttcgacaca ggtggggggg cgtatcgatc
     2041 aactgccaac cttacggccc ccaactgctc gtcgacggac ggagtggtgg tttataaacg
     2101 tcaagcagca ggttcgtggc ccgcggaact ggggccatcg gctgcgggtg ccaccggcgt
     2161 tgtgagttgc ggttttggca atggtgccaa cgggtatcca attttagcgg ccggtatgat
     2221 ggaatttaat ttcgtaaatt attttcgtga aggcaccact gcgctgctcg atttacaaac
     2281 cgtagcagtg cacgagttgg ggcatttgct tgggctcgat cacgcgtgcg ggcctattaa
     2341 aaatggaaaa ccaaacgcgt cttgtccaaa gtatctttcc gccgttatgt accctagcgc
     2401 attctatgat tccgctaccc gtgtcggaca aatcaaacga aacctcgata ccaatgatca
     2461 gggacgagcg aattgtcttt attgagcttt cccaacaagt ggtgaaatta ctcgacaatt
     2521 ttacgcgctt tcaacaaagc gtaagctgcg tcgtttccgt atacattggg tataaaatta
     2581 atcgagcgag aaagtttact taccaacgta ttttctcgtt ccactagcaa agaaatttta
     2641 ccgtgttttg aaactatctt aaccgggtca cgttcggtgt aaagctgact gcgatcttta
     2701 cgcgtgccca tgagactggc gtaatctttg gtaaaaatga cgtctttcga cggaacatcg
     2761 gcaatgatcc attcccgccc tttgccatgt tttgtaatta agtgctgaat ttcttggatc
     2821 ttagcattta atttttttac ggcctcttcg cgcggggagt tttttccagt gccagtttcg
     2881 agaatgcggt gttcgctttc ttttaggcga atggtctttg gaggaacacg atatatcagc
     2941 atgcgcattt gttcgttgac gatcggatct ggaattcgtt tggaccagta gaggtcctga
     3001 attcgcgaca taaaatacac gtcgttaaaa ccaaaaaacc gttcagggtc cttggccgct
     3061 agctctaaaa gttcgtgata gtggtaaatt aaattatttt ttaagagata tgacgcaata
     3121 tgcgctgcta aaatgtcgaa ttttgcggaa ctcgagttac gaattacctg ggagtaccaa
     3181 gcgaatcttg cgatcaagaa gtcttccacc gcatgtacgg cgttttcgcg tactgcgaga
     3241 atttcttttt ttccatctgt gaacgcagtg gtcaaatgat agagaatgta atctttatcg
     3301 atgtgaccat acttgatgcc agtgtggtga gcgtcgcgaa ctaagtaatc gagccgatcg
     3361 gcatcgatgt cggaatgcag aatttgattc gctacgagat tgcccgaatc acctttgata
     3421 aatttggaga gcgacgtaaa atctaagcca ctttccttta aaatacgggt gattcccccc
     3481 ttgaagtcag tatttttaat caaataagag cccaggtgct cgtgattatt tggcaactgt
     3541 ttgccgggga gctcacctcg ttggcgcagt gactctccat atttgatgta agaaccttcg
     3601 atcgaatgag aaaagggaaa cgtgccgatg tcgtgtaaca ttgctgcaat gcgaatgctt
     3661 ttgtgaaaca tcgaattggc atcggtggtt ttggtgctgt agagttgagc atcgggtacc
     3721 ccacgcccga tggcacgcac catttggtcg gcgcagtgaa gtactccgac tgcgtgagca
     3781 aaacgggtgt gttcggcacc gtcaaaaata taggaagcaa aacccagctg cttaatccag
     3841 cgaaggcgct gaaaaaaagc tgaattgata atttcttctt ctgttttggt taaacgaata
     3901 aatccgtaga tgctatcgta aatttggccg gctctaaagc tttcgggtcg atgtgctggt
     3961 cttgccatga gttccactct ccggttccca ttctcgcaga aattttgggc ttcgtcacct
     4021 ggcacgctcg ttgctaagtg catgctgaga ataattaatg tcgaggaaat tttatgtttt
     4081 tgaagttcaa ttcaacgagt agttcagtgc tgagtttggg tgttttagca gctctcgttt
     4141 cgatactcag cggctgcggg cgggagctca agattcgagt agctccgagc gaagaggttc
     4201 gccacagcgc cgcttcggat ttggtgcgac aagtggagat acgctaccgg aatgcggctg
     4261 gggaggagcg ttttcgagaa tcagtctcgg tcaccggact tggtcagcca ttagacatta
     4321 aaaacgaatc tatccaggat ctggactggt caaaagatgg gcgctgggaa gccaacgctt
     4381 tcaacgggga cggcgcattg atgttgcacg catctaccca ggcagaccca gaggaagacg
     4441 aacagatcga cctagtgttg gctcgggctt cgggtccaca agcgccggca aacctaaatt
     4501 cggtcttttt gaagtcgttt cgtacgaccg gctttagcgt ggacacaaaa catagtgtcg
     4561 aaaagttgcg aagcccagtc tgttatgaat tgcccgcaat atatttagac gcaggatctg
     4621 gggcactggc tcgtggtgta tttttcgaaa caagcgttgc cggcacagcg gtgcgggtgg
     4681 ccatgagcaa ggacggtgag cggcgggtac tccctgttca ttcgagcggt gacagttatc
     4741 ttgatctagc tgatgaaatt gaaaaaatcg gatcaaccag gcgtagaaat ctttatatat
     4801 ttaccgacga atacgaaggt tgcgtaacct tgagtgccgg attatcaaat ctgcttgacc
     4861 cgactctgac gtacaagttt ctttacgagg caggccgaaa cgtcggcatg gggaagggca
     4921 tggccggtga aattgtcgtt ctcaacccga accccatcga ggtcgcggtc gcggtcaatt
     4981 gttctatcta ttcgaacgct caagatccaa acggcatttt catagggcct ctcggtcgtg
     5041 atcattacgt atataaagag gagtgccaaa gaaccaatgc caatcattcg ttcacccgct
     5101 ctggcgtcat cgccaaacgc gatacatttc gagtgccggt gtgcgggtac gggaacctat
     5161 acggggaaag tcaggggcat tcagggcttg ttcataccgg ttttaccctt cagtgcagcg
     5221 caagactcga agtcgacggg ttgatcggct cgtcgccaac tgtgtttcta acggcaccaa
     5281 agtaagttac agttctaggc atggaatcca atttttcgat acgtcctgcg accgaagacg
     5341 atctacccgc gatcctcgaa atcgaaaata gtgtttaccc aattccttgg accgccgaac
     5401 agtttaaggc cgaaatcgaa aaaccgttcg cgaatgtttt ggttctcacc gacgacgaca
     5461 ccgaccagat tgtggccggc tacgtcgttt tctggatgat gttcgacgaa tgtcacattc
     5521 tcaacgtcgc ggttcatccg aattggcggg ggctcggaat cggaggtaaa ctcacccgaa
     5581 tagcagtaga tttcgcttta aaaaaggaac tcaagcgggt gtttttggaa gttcgaaagt
     5641 ccaacgaggc ggccgtgaag ctctatcaga aggtcggatt tttcattgat cacatcaaac
     5701 cacgcttcta cgacaacggg gaagatgcct atttcatggt gatgtaccta acccgaccaa
     5761 atcattttta attcaatagt atcagtggtt aaagtccttt attccgctgc tgtagcgagt
     5821 ttccggttgg cgctcgacgc tcccgcgttt gacttttttg ggccgctttg tgtaagttgc
     5881 gcgctttact atgtatcgag ctttaaaaaa cggacttatt ctttcaagtt tttttgccgt
     5941 ggttttcgtt ttgaaacccg ctgatcaggc ggcagaacgc gcttggctgg cgtcgctttc
     6001 ggaccctaaa gaagcgctgc tcgcaaaaca acattctaag cggtcgaaaa aggcctttgg
     6061 cgccaaagag gttcgtcgat ttttagagcg ccaaattaaa caggcttcgc ccgagcaatt
     6121 ggattcgatt acccaagtca tattagaggt ctcgaagtcg taccattttc atccggcgct
     6181 cattctttct ttaattcgag tagaaagtcg gttcaacgcc tgggccatta gtccaaaggg
     6241 tgccattggt ttgatgcaat tgatgccgga aacaggacgc tggttggccg atcgcatggg
     6301 cgctcgctgg gacggtccag ctatgttgtt ggatcccgag ataaacattc gatttgggac
     6361 ttactacctc gcctatctgc gtgagcgata taacggtgac ccaaagtttt atctctcggc
     6421 gtacaacgtc ggtccgggtc ggatggacgg ttgggcaacg accgggtata a
//
LOCUS       DAOMXO010000040         6243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6620_length_6243_cov_4.287007, whole genome shotgun sequence.
ACCESSION   DAOMXO010000040 DAOMXO010000000
VERSION     DAOMXO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6620_length_6243_cov_4.287007"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PLH57_01580"
     CDS             <1..850
                     /locus_tag="PLH57_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK01330.1"
                     /translation="LEESGDYALARNIYSALLKIGAKLAEAHAGLASTFEKEGLFEQS
                     IRHYREAIAYASELSFYQSLAALQIRTGDDKEAAKTLIHALGLGNIDEATRFELHKSL
                     GNCFTRSGDYGKAEHHYARAYEYQPKSDTLQVNVGSLALQKNDISAALEHFQKAIELN
                     PNNDKAICGVGMVHLSNGDIERAYDYFLAAVRSQPSNITALFNLAKCALDLRKYSDAA
                     EALENYMKNSAPNVGILFSYAGILFHKGDIANASKEVERILKINASHAGAIELRDIIA
                     RRNKNE"
     gene            953..2599
                     /locus_tag="PLH57_01585"
     CDS             953..2599
                     /locus_tag="PLH57_01585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRK01331.1"
                     /translation="MKKILIIQLARFGDILQTLPSIQGLKSKYPECEITLLVRSTFRD
                     AALLSPFVDHLIEFPTREILGPYFSEPSDDNKAVTLAHLIRFLANDFMRQKYDLCLNL
                     TFSKSSSLLTQMITATEKRGLQRSRDHEVAVKDPWSQYFLAQVSNRNLNILHLNDLFA
                     RICGVEDYSYPVQLGATSMPRDVEAPEAKKKYLGIQIGASVAGKTLDVATWKTICEYL
                     KKSFGSHTLVFFGSSDEKHSIHAIAPNESIVLAGKLRLQENVRWIRACEHIVTPDTAM
                     VHLASLAGTNVINISLGGVRAQETGPYGEGHSVLIPKSPKSSDYAKAIQAIIAGDAVG
                     TSFFHYKSTLRKTSFGSVRSELQNFNFQIDETRNFFEQSFYLLAEFRCAGRIDDLPIP
                     VLRDSAHEPGSNAKSQVDRLLHAFDAMCALKKIGKMGQQFAVDLILHSRDKRSMKKLT
                     RDLEELDRLMNELHGSSEYVRPLIDWVRVTRDFVDCSQNAQERLFELAGAQEGAYREL
                     EQNVDILEQLLQTAVNAATKRVTKTSKSTQVDTKRGLDAE"
     gene            2589..3194
                     /locus_tag="PLH57_01590"
     CDS             2589..3194
                     /locus_tag="PLH57_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01332.1"
                     /translation="MPSKHFIMNGEEREFPVGSARFSEFLSSLSQDLKSSEQVISLIR
                     IDGQEISDEQEGFFRNKTMQEIGQVEIFTSSPTELAHETLITLDQYIVKVIETLNKAA
                     DGYKSKNYLAADAFFVRAIDSLDLFVQTIGGIKLALRIGLHTKIAVVEASLVSIMNDL
                     LTAKRENNYIYLAELLEKDLVENLGEWKQDVFPILKNWKVS"
     gene            3196..4074
                     /locus_tag="PLH57_01595"
     CDS             3196..4074
                     /locus_tag="PLH57_01595"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRK01333.1"
                     /translation="MAKIRCVGIIIKQKVSEANELGLEVAAYLKKRKIKVAFASESKE
                     VCDRALGVRCTSKEALINISDMIVVLGGDGTFVSVARLMKKKSVPLLGVNMGQLGFLT
                     EFKKTELFEGLSAALRGKLPISERTMLECTLVRRGKVLAREPVVNDVVVSKGAIARIF
                     DMEVSVNGKLVTNIKADGLIVASPTGSTAYSLAAGGPIIEPSVPAIIIAPICPHSLTL
                     RPLVLGDLSKLSVLPRFKSGSVVLTLDGQKSFELQPNDLIRVTKYGKHPLKILRWPKR
                     DYYALLREKLKYGYRD"
     gene            4114..5811
                     /gene="recN"
                     /locus_tag="PLH57_01600"
     CDS             4114..5811
                     /gene="recN"
                     /locus_tag="PLH57_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRK01334.1"
                     /translation="MLRLLRIRNFAIIDGLELEFHGGLNVITGETGAGKSIVLEALGL
                     VLGNRAETELIRNGCNEAVVEALFDLAKHPSVTARLEKQGIDLQKNDFELLVKRTIQR
                     NGKNRIYLNDDLITLGQLADLCENLVDLCSQHEHQSLAKPIFQLELLDRYGGLGELRN
                     EVRESYSALRSLESELISLKSKINNNESQKDFIRYQINEIDAVAPVENEDMLLQDEHR
                     RLRNIGTIHEGCRNILDLTRNNQESQNGMGAFDQVGRIRQRLLKLAEQDAGLLELLPL
                     TEQIQEELAQLANAIEAYSERIDGDPERLHLLEDRLTKINQLKRKYGSTISDILETRN
                     RLSEKLEETANIEVLIHEKQAKFDLIRADYIKTASILSKKRQNVARTLKASVTKELTE
                     LRMPDTSFEIDFSRVDPESIGSVDGVDRIQFLFSANAGVEPRPLAKVASGGELSRLML
                     ALRRTLGDRGGIGVYVFDEIDAGIGGQTGTLVGRKLKDVASHNQVLCITHLPQVAAFA
                     NAHYVVEKTTISAVTMSIVRHLDDKNRVEELARMLGGAQVTAKSRAHAQELIRDSSV"
     gene            complement(5788..>6243)
                     /locus_tag="PLH57_01605"
     CDS             complement(5788..>6243)
                     /locus_tag="PLH57_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01335.1"
                     /translation="NGRKILFSHGDKINAKDYGYQLFRFGLRNLVSQTILEVAPGSLI
                     DRIGSSMSRASRGYHPEPDEKVVQLFRNYACEQISKGYDFVVLGHSHYMDDMHFCVED
                     HKGQYVNVGYPRKHCKYLELTADAPTFKFLNWDDVVIPFRSSSNRTVSD"
BASE COUNT         1849 a         1414 c         1382 g         1598 t
ORIGIN      
        1 acttgaggaa tctggcgact atgctcttgc gcgaaatatt tattccgcac tcttaaaaat
       61 aggcgcaaaa cttgcggaag ctcacgcggg cctcgctagt acattcgaaa aggaaggtct
      121 tttcgaacaa tcgattcgtc actatcgcga ggcaattgcc tacgcatctg aactttcgtt
      181 ctatcagtcg cttgcggcgc ttcaaattcg cacgggtgat gacaaagaag ccgcgaaaac
      241 actcatacac gccctgggtc ttggcaacat cgacgaggcc acgcgctttg aactgcataa
      301 atcacttgga aactgtttca ctaggtcggg cgactacggc aaagcagagc accactacgc
      361 tcgtgcctat gaataccaac caaagtccga cactttgcag gtaaacgtag gcagcctcgc
      421 actccagaaa aatgatatct cggccgcgct tgaacacttt caaaaagcca tcgaacttaa
      481 ccccaacaac gataaggcca tttgcggtgt tggtatggtg catttgagta atggggacat
      541 tgaacgggcc tatgattatt ttctcgccgc cgttaggagc cagccaagca acattaccgc
      601 gctattcaac ctcgcaaaat gtgctctaga tcttcggaaa tacagtgatg cagcggaagc
      661 cttagaaaac tatatgaaaa attccgcacc caacgtcgga attcttttta gttatgctgg
      721 cattttgttc cacaaaggcg acatagccaa cgcctcaaag gaagttgaac gaatactaaa
      781 aatcaacgct agtcacgccg gagctattga gcttcgtgac attatcgcgc gcaggaataa
      841 aaatgaataa gaacgacaac tcaaaaaaag aactcgaaga aatgctaacg tcgatcgata
      901 aaacgttact cctcaaacta ctcgacgcat tgatcgagga gcaaaactcg aaatgaaaaa
      961 aatcttaata attcagctgg cgagattcgg ggacattttg caaaccctgc cgtctattca
     1021 aggccttaaa tccaaatatc ctgaatgtga aattactttg ctggttaggt ctacctttcg
     1081 agacgccgcc cttcttagtc cattcgtaga tcacttgata gaatttccca cgagggaaat
     1141 cctgggaccc tatttttccg aaccgagtga cgataacaaa gcagtcacgc tggcgcattt
     1201 aattcgtttt cttgccaacg atttcatgcg gcaaaaatac gatctttgcc tcaatctaac
     1261 tttttcaaaa tcaagttcat tgctcaccca aatgattacc gctactgaaa aacgaggact
     1321 tcaaagatct cgcgaccacg aggtagccgt aaaagatccg tggagccagt attttctcgc
     1381 ccaggtttca aaccgaaatc tcaacattct tcatctgaat gatctattcg cacgtatttg
     1441 tggcgtggaa gattattcat atcctgtgca attgggggct accagcatgc ctcgcgatgt
     1501 cgaggcgcca gaggcaaaaa agaaatatct cggcattcag atcggtgcga gtgttgctgg
     1561 aaaaacactg gacgttgcta cttggaaaac aatctgcgaa tacctaaaaa agagcttcgg
     1621 tagtcataca ctagtttttt tcggttcatc ggacgaaaaa catagcattc acgctattgc
     1681 gccaaatgag tctattgttt tagcgggtaa actacgactt caggaaaatg tacgctggat
     1741 tagggcatgt gaacatatag taaccccaga cacagcgatg gtgcacctcg cgtcgcttgc
     1801 aggaactaat gtaataaaca tttcccttgg tggtgttcgc gctcaagaaa ccggaccgta
     1861 cggcgaaggt cattcagtac taattcctaa gagccctaaa tcttctgatt acgcaaaagc
     1921 gatacaggca atcattgcgg gtgatgcggt tggaacttct ttttttcatt ataaatccac
     1981 gctgcgcaaa acttcattcg gaagtgttcg ttcggaactt cagaacttca actttcaaat
     2041 tgatgaaact cgcaattttt ttgaacaaag cttctatctt ttagctgaat ttcggtgtgc
     2101 tgggagaatt gacgacctac ccataccggt acttagagac tccgcgcacg agcccggatc
     2161 taacgccaag tcccaggtag atcgactgtt gcacgcattt gacgccatgt gtgcacttaa
     2221 gaaaattgga aaaatgggtc agcagttcgc ggttgatcta attctccatt ctcgcgacaa
     2281 gcggtctatg aagaaattaa ctcgcgattt agaggaactc gatcgcctca tgaacgagct
     2341 tcatgggtcg agcgagtacg ttcggccact gatcgattgg gttcgtgtga ctcgggattt
     2401 cgtagactgc tcacaaaacg cccaagagcg tttattcgag ctggcggggg cacaagaagg
     2461 agcctatcgt gaactcgaac aaaacgtcga cattctcgaa cagctattac aaactgcagt
     2521 aaatgcagcc acaaaaagag tcacgaagac aagtaaatca acgcaggtcg ataccaagag
     2581 gggactagat gccgagtaaa cattttataa tgaatggtga ggaacgcgag ttccctgtag
     2641 gttctgcacg attttctgaa tttttaagct cacttagcca agacctcaaa tcatccgaac
     2701 aagttatttc cctaattcgc attgacggtc aggaaatcag tgatgaacaa gagggttttt
     2761 ttcgaaataa aacaatgcag gaaattggcc aagtcgagat ttttacttca agtccaactg
     2821 aattggcgca tgaaactctt atcacgctag atcaatatat tgttaaagtg atcgaaactc
     2881 tgaataaagc tgccgacggg tataaatcta aaaactatct cgctgccgat gccttctttg
     2941 tgcgagcaat cgactcgctc gacctttttg ttcagactat cggcggaata aaacttgctc
     3001 tccgaattgg actacacacc aaaatagcag tcgttgaggc gagtctcgtc agtatcatga
     3061 acgacttatt gactgctaaa cgcgaaaata attacatcta tctcgccgaa ctgctggaaa
     3121 aagaccttgt cgaaaatttg ggcgaatgga agcaggatgt ctttccaatt ctaaaaaact
     3181 ggaaggtatc ttgatttggc aaaaatccgc tgcgttggaa tcattattaa gcagaaggta
     3241 agtgaagcca acgagctcgg acttgaagtc gcagcttatt taaaaaaaag aaaaataaaa
     3301 gtcgcctttg cgtctgaatc taaggaagtt tgtgaccgag cccttggagt tcgttgtaca
     3361 agcaaagaag ccctcataaa catttcggat atgattgttg tgctgggcgg cgacggaacc
     3421 ttcgtcagcg ttgctcggct catgaaaaaa aagagcgttc ctttgttagg ggttaacatg
     3481 gggcagctcg ggtttttaac cgagttcaag aaaaccgaac tattcgaagg cctttctgct
     3541 gcgcttcgcg gtaaattacc tattagcgaa cgaaccatgc ttgaatgtac tttggtgcga
     3601 cgcggaaagg tattggctcg agaaccagtt gttaacgatg tggtagtcag caagggcgct
     3661 atagcccgca ttttcgatat ggaagtctca gtcaacggaa agctggtaac aaacattaag
     3721 gccgatggcc ttatcgtggc atctcccacg gggtccacag cgtattcatt ggctgcgggt
     3781 gggcccatta ttgaaccgtc ggttccagca attattattg cccctatttg ccctcattct
     3841 ctgaccctca gacctctggt cttaggcgat ctttcgaagc tttcggtcct accacgtttt
     3901 aaaagtggca gtgtcgtttt gacgctcgat ggtcaaaaaa gctttgaact tcagcccaac
     3961 gatctaattc gcgtcacaaa atacggaaaa catccgctaa aaatcctacg gtggccaaaa
     4021 cgcgattatt acgcgctgct acgcgaaaag ctaaaatacg gttaccgaga ttgattggtt
     4081 ttggtagttt gagcccagga atgggatcaa acgatgctac gtctacttag aattcgcaat
     4141 ttcgcaataa ttgatggact cgagctcgaa tttcacggag ggctcaacgt aattacgggt
     4201 gaaaccggtg ctggtaaatc catcgttttg gaggctctcg gtcttgtact tggcaatcgc
     4261 gccgaaacgg aattaattcg caatgggtgc aacgaagcag tcgtcgaggc cttatttgac
     4321 ctggccaaac acccctccgt gacggctcgt ttggaaaagc agggaatcga cctgcaaaaa
     4381 aatgattttg aactattggt aaagcgaacc attcaacgca atggaaaaaa tcgaatctac
     4441 ttaaacgatg atttaattac tttgggtcaa cttgccgacc tgtgtgaaaa tctagttgat
     4501 ctttgttcgc aacacgaaca ccagagccta gcgaaaccta tttttcaact agaacttctc
     4561 gatcgctacg gaggactcgg tgaacttaga aatgaagtta gagaatccta ctctgcattg
     4621 cgttctctcg agtcagaact gatttcatta aagtcgaaga tcaacaataa cgagtcacag
     4681 aaggatttta ttcgatatca aattaacgag attgacgctg ttgctccagt agaaaacgaa
     4741 gatatgcttt tacaggacga gcatcgaaga cttcgtaata ttggaaccat tcacgagggc
     4801 tgccgaaaca ttctcgatct gactcgaaac aatcaggaat cccaaaacgg aatgggcgca
     4861 tttgatcagg tcggtcgcat tcgtcaacgg cttcttaaac tagcggaaca agacgccggt
     4921 ctgttggaac tactcccact aacggaacaa attcaagagg aactcgctca attagcaaat
     4981 gccatcgaag cttactcgga acgaatcgac ggcgaccccg aaagactgca cttactcgaa
     5041 gatcgactga ctaaaattaa tcaactcaag cgcaaatatg gttccacaat ttctgacatt
     5101 ctcgaaactc gaaaccgtct ttcagagaaa cttgaagaaa ctgcaaatat tgaagtattg
     5161 attcatgaga aacaagcaaa gtttgacctg attagggcag attatatcaa aactgcatcg
     5221 attctttcga aaaaaaggca aaacgttgct cgtactctaa aagccagcgt caccaaagaa
     5281 ctcaccgaat tgcgaatgcc agacactagc tttgaaattg acttttctag agttgacccc
     5341 gagtcaatcg gttctgtcga tggagttgat cgtatacaat ttcttttttc ggcaaatgcc
     5401 ggagtggaac cgcgtcctct cgctaaggtg gcgtcggggg gggaattgtc ccgtctcatg
     5461 ttagctctta gaagaacttt gggtgatcgt ggcggaattg gcgtgtacgt atttgacgaa
     5521 attgacgccg gaattggcgg acaaacagga actcttgttg gaaggaagtt aaaagacgtg
     5581 gcgagccaca atcaggttct ttgcattact cacttgccgc aagtagcagc ctttgcaaat
     5641 gctcattacg tggtcgaaaa aacaacgata tcggcagtaa ctatgagcat tgtacggcac
     5701 cttgatgaca aaaatcgcgt cgaagaactt gcacgaatgc tgggcggagc ccaagttacg
     5761 gctaaatcac gtgctcatgc acaagaatta atccgagaca gttctgtttg aactcgatct
     5821 aaatggaatt acaacatcat cccaattcaa aaacttaaaa gtcggagcat ctgccgtgag
     5881 ttctaaatac ttgcaatgct tccttggata cccaacattt acgtactgac ccttgtgatc
     5941 ttccacacaa aaatgcatgt catccatata atgggaatgc cctaatacta cgaaatcgta
     6001 tcccttcgaa atttgttcac atgcgtaatt tctaaatagc tgaactactt tttcatctgg
     6061 ttccggatga tagccacggc ttgcccgcga catagaactt ccgatgcgat ctattaaaga
     6121 tcctggtgcc acctcaagga ttgtctggga aaccagattc cgtaatccaa aacgaaaaag
     6181 ttgataacca taatccttag cattgatttt atctccatgt gaaaaaagaa tttttcttcc
     6241 gtt
//
LOCUS       DAOMXO010000041         6138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6783_length_6138_cov_4.792372, whole genome shotgun sequence.
ACCESSION   DAOMXO010000041 DAOMXO010000000
VERSION     DAOMXO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6783_length_6138_cov_4.792372"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PLH57_01610"
     CDS             complement(<1..121)
                     /locus_tag="PLH57_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRK01336.1"
                     /translation="MAGESNISLEKVRNIGISAHIDSGKTTLTERILFYTKKIH"
     gene            complement(187..1515)
                     /locus_tag="PLH57_01615"
     CDS             complement(187..1515)
                     /locus_tag="PLH57_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01337.1"
                     /translation="MALIPARATASGSKSEVKSEVDFNWTEVEGATHYVVTIAKDPTF
                     QKVITNIDTAYTKARSQPLPPGAYYWTVDAFNDLYHLHRLAPRPIVVGSPYQITGESL
                     VKLPAPQTLSPPDQALYPPLAKIRLAWAQVPGAKKYLFRLWDEDHRVYHTQIKKESHL
                     RWETTVDRTWIVVHDAPYSSFIYLDPGDYRWEVAGVNEDNHQMGEFASGRFEVNKKYF
                     FKPRVHELSLFGITSPRYFVRFGSLGSNANIKVMTIPFGGGGAWNWWGSRVSGLRLTA
                     SVVSVIRRHTMRKIALEGRFRYKLSNVPKAWHFEFGIGPSFLQLPHIDVNSITRDETV
                     DLLSTLGPLAELALVYQFESPYTNTLGFRTGLPMLLIRGQGEGGRIVRGVDMRVFNEL
                     RWHTSANGFLSGSLELFYSEVQFRPNGVSEGRSIARYIGPSIRLGLGLVF"
     gene            1674..1937
                     /locus_tag="PLH57_01620"
     CDS             1674..1937
                     /locus_tag="PLH57_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRK01338.1"
                     /translation="MFGLSGEHILILAGILLVFGPRKLPELGSSVGKALKNFKEGLNG
                     PASSGKLEARTVEARNSEASATVDPVATAQKATATAATDRPNA"
     gene            1972..3198
                     /locus_tag="PLH57_01625"
     CDS             1972..3198
                     /locus_tag="PLH57_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRK01339.1"
                     /translation="MHLEILLALALAIFLGRVMALVSERFSLPGVIGEISAGLLLGNL
                     ILVMPAFSLSRVYSESEFLHGLSELGVIVLLFSVGLESSISDLKKVGFRATLVAIIGV
                     VVPLLATLAAWKFGIVEDRLPSGEVSYLVPLFIGAAMTATSVGITARILSDVGKLRSA
                     TGQLILNAAVIDDVLGLLILGVVGALATHMVGAEASLSGGANQSMGWLIATIVGKALA
                     FFAVVLFLGIAVFGRLRGYVRKIPQGIVTLGLVTCFSFAWLAQLFGLAAIVGAFFAGL
                     LLDDATDEVESYFEPLVKFLSPLFFILIGASVKIDSFTSLGWFSFFEVGLFVLIAIFG
                     KMACSLVAGKGVDRVAVGLGMAPRGEVGLIFAAAGKTLGVFSDEVFGVLVFVVLLSTL
                     IPSVLLDRWFRRPLAR"
     gene            3188..4240
                     /locus_tag="PLH57_01630"
     CDS             3188..4240
                     /locus_tag="PLH57_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRK01340.1"
                     /translation="MRVDRLSANRVFGLLERNMRKAPRWNALERADAIFEETCRNFVE
                     LLGAKRVAVYLDDPLTKSGDKIEEVRLVCVAAVGAEAGSRVPVGGGPLGLCYRQGRMV
                     RGGAILYVPIVMGRSTVGVVEAKLKTTIAEDTRSKTANARGRLAQTLCSYFSAALQNV
                     LDAQKKLERERRDALTGLASGQELDLFLRARVGKATASRPVSVIFLDLDFFKSVNDTW
                     GHLVGSQLLADIGESLRRTLVQFGRNYKKNFESLVARYGGDEFVVVLMGPNVDDSLGL
                     AETIRARIASKKGLSRPITASLGLAWTGSGVRRRDYRALLKAADEAMYEAKRLGRNRV
                     VVSGKGDRHEFTQKFT"
     gene            4215..5627
                     /locus_tag="PLH57_01635"
     CDS             4215..5627
                     /locus_tag="PLH57_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRK01341.1"
                     /translation="MSSPKSSLETALKQYEKTKNQHLSDLKELVRIPSVSFDGFDPKN
                     VQRSAEAVAALLKKRGLENVEIIKLADDVHPYVYADWLHAGPNAPTLLLYAHHDVQPP
                     GRMEIWKSAPFEPVERDGRLYGRGTADDKAGVIVHSAAIASYLETAGKLPVNVKLVIE
                     GEEEIGSTHLKEFLAKYKQKLKADVMVLTDTQNYDTGLPALTVSLRGLVVFEVEVKAH
                     ERSMHSGMWGGPVADPVIALSRMIATMTDAEGRLTVPGIWDEVRPVTKEERAEMDRLP
                     FTIQEFRYQAGLLPSTRLLPGKDGTVHPLLQMWREPSLSVNAVEASSRKAPANIINNS
                     AWAQLGVRIVADMDPVRTARLVEEHLKRVAPWGVEVSVKTDIGGKAWGTRATGPVFDA
                     AMRSLEKGYGKRPVLMGCGGTIPFVEPFSDALGGAPAILIGVEDPYTQAHSENESMPL
                     ADFEAAVRSAIYFYDELGNR"
     gene            complement(5666..5959)
                     /locus_tag="PLH57_01640"
     CDS             complement(5666..5959)
                     /locus_tag="PLH57_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01342.1"
                     /translation="MGKKKKFDLSHLVHEGYLKDGDSISFVSDPSKTAKIMKQPNGEY
                     KLKVGKETVTTLHAYSMELLGQEPPNHASCWFKAPNGKTLYELWHADDYVEAA"
BASE COUNT         1388 a         1417 c         1763 g         1570 t
ORIGIN      
        1 cgtgaatctt ctttgtatag aaaagaattc gctcggtgag tgtagtttta cctgagtcga
       61 tgtgtgcaga aattccaata ttgcgcacct tctcaagaga gatgttcgat tcgccagcca
      121 tgaaaaagct ccttaattaa tggattgaac ggctacgttt agcgaattct cgattgacga
      181 tcaaggttaa aaaaccagac cgagccccaa gcgaatggac gggccaatat agcgggcaat
      241 cgaacgcccc tcggacactc cgttgggtcg aaactgcacc tcgctgtaga agagttcgag
      301 cgagccggaa agaaaaccgt ttgcgctagt gtgccagcgc agttcattga aaaccctcat
      361 atccacccca cgcacgatgc gcccaccttc gccttggcct cgaatcaaca acatgggcag
      421 tccggtgcgg aatcctagag tattagtgta gggagattcg aattggtaca ccagcgcgag
      481 ctcagccagc ggtcccagag tagataacaa gtctaccgtt tcgtcgcggg tgatggagtt
      541 gacgtcgata tgaggtaatt ggagaaaaga tggtccgatt ccgaactcga aatgccaagc
      601 tttcggcaca ttagagagtt tgtagcgaaa tcgtccttca agagcgattt tgcgcatggt
      661 gtgtcgacga atgacgctca ccacactggc cgtcaaacgc aatccggaca ctctggatcc
      721 ccaccagttc cacgcaccgc cgcccccgaa aggaatggtc atcactttga tgtttgcgtt
      781 tgaacctagc gaaccaaacc gtacgaaata gcgggggcta gtaatgccaa atagactcaa
      841 ctcgtgaacc cttggtttaa agaaatactt cttgttcacc tcgaatcgac cagaagcgaa
      901 ttcgcccatc tggtgattgt cttcattgac tcctgcgact tcccaacgat agtcgcccgg
      961 atccaaatat atgaacgacg agtacggggc gtcgtgcacc acgatccaag ttcggtccac
     1021 ggtggtttcc caccttaagt gagactcttt tttgatctgg gtgtggtata cgcggtggtc
     1081 ttcgtcccag aggcgaaata ggtatttctt ggcgccggga acctgggccc aagctaatcg
     1141 aattttggca agtgggggat acagcgcttg atcgggcggg gagagcgtct gaggggccgg
     1201 cagtttgacg agcgattcac ctgtgatttg gtatggcgac cctacgacga tggggcgggg
     1261 cgctaaacga tgaaggtggt acaggtcgtt gaatgcatcg actgtccaat aataagcccc
     1321 cgggggcagt ggctgggagc gcgcttttgt gtaagccgta tctatattgg taatgacttt
     1381 ttgaaaggtg gggtctttgg cgatcgttac gacgtagtgg gtggccccct ccacttcggt
     1441 ccaattgaaa tcgacctcac ttttgacctc ggatttagag cccgaggcag tggcccgcgc
     1501 cgggattaag gccacgaaac acccaagaac gaaaaaatgg aaaatgcgac ccgcctgcct
     1561 catagactct attattcagg agaaactcgc cgagagcaat tccttgattg acgtggctgc
     1621 tcggattata agaaatggac tgctcgtttt aatggtaagt tgtggaggtt ccgatgtttg
     1681 gattaagcgg cgaacatatt ttaattttgg ccgggatttt gctggttttt ggaccgcgaa
     1741 agttgccgga actgggttca agcgttggca aagcgcttaa aaactttaaa gaaggtctca
     1801 acggtccggc gagctctggg aagctggaag cgcgaaccgt ggaagctcga aatagcgagg
     1861 cgagcgcgac agtggaccca gtggcgacag ctcaaaaagc aaccgccacc gcagccactg
     1921 atcgtccgaa cgcctagttt acattttatt gagcgcgcct gagagggaac gatgcatctt
     1981 gaaatacttc tggctttagc attggctata tttctaggcc gcgtgatggc gctcgtgtcc
     2041 gagcgttttt cgttacccgg tgtgattggc gaaatcagcg ccggtctttt gttgggtaac
     2101 ttaattttgg tgatgcccgc gttttcgctt tctcgggttt attcggagag cgaatttctt
     2161 cacggtttat cggagttggg tgtcattgtt ttactttttt cggtaggcct tgaaagttct
     2221 attagcgact taaaaaaagt cggatttcgt gcgaccttgg tcgcaatcat cggtgtagtg
     2281 gttccgttgc ttgcgactct cgcggcctgg aaatttggta tcgtggaaga ccgtctgcct
     2341 tcgggcgaag tgtcttatct tgttccgctt tttatcgggg ccgcgatgac cgccaccagc
     2401 gttggaatta cagccaggat tctttcagat gtaggcaagt tgcgttctgc taccggccag
     2461 ttgatcttga atgctgcggt cattgacgac gtactcggat tgttgatctt gggtgtggtc
     2521 ggagcgctgg cgacgcacat ggtaggggcc gaggcgagct tgagcggagg agctaaccag
     2581 tcgatgggct ggctcatcgc caccattgtg ggcaaggcgc ttgcgttctt tgcggtcgtg
     2641 ttattcttgg gtattgcagt atttggacgc cttcgtgggt acgttcgaaa aattccgcag
     2701 ggaatagtga cgcttggact agtgacctgt tttagttttg cgtggcttgc acagttgttt
     2761 ggtttagctg cgattgttgg tgcttttttt gcgggtttac ttttggacga cgccacggac
     2821 gaagtggaat cctattttga accattggtg aagtttttat ccccattatt tttcatactc
     2881 attggcgcga gcgttaaaat tgattccttt actagtttgg ggtggttttc gttttttgaa
     2941 gttggactat ttgttctaat cgccattttc ggaaaaatgg catgttcact ggtggcgggc
     3001 aagggcgtcg atcgtgtggc ggttggactt ggaatggcac ctcggggtga ggtgggtttg
     3061 atttttgctg ctgctggaaa aacgcttggt gtttttagcg acgaagtgtt cggagttctg
     3121 gtattcgtcg tactcttgag cactttgatt ccgagcgtgt tactggatcg ctggttccgg
     3181 agaccccttg cgcgttgatc gtttatcagc caatcgagtg tttggtttgc tcgaacggaa
     3241 catgcgaaaa gcaccacggt ggaacgcact cgagcgagcc gatgcaattt tcgaagaaac
     3301 ttgtcgcaat ttcgtggagc ttcttggggc caaacgggta gcggtttatc tcgatgaccc
     3361 tttgactaag tccggtgata aaattgaaga ggtgcggttg gtttgcgtgg ccgcagtggg
     3421 tgcggaagct gggtcgcgtg ttcccgtagg tggtggacca ctggggttgt gttaccggca
     3481 gggacgcatg gtgcgcggcg gggcgatttt gtatgtgcca attgtaatgg ggcgatccac
     3541 ggtgggagta gttgaagcca agctcaagac cacgattgcc gaagacaccc gctccaaaac
     3601 cgccaatgcg cgtggtagat tagcgcaaac tctttgtagc tacttttcag cggcacttca
     3661 aaacgtgctc gacgcccaga aaaaactaga gcgcgaaaga cgcgacgcac tcaccggatt
     3721 ggccagtggg caagaactcg atttgttttt gcgggcccgc gtgggcaagg ccacagcgag
     3781 tcggcctgta tcggtcatat ttcttgacct cgattttttt aaatcagtca acgacacctg
     3841 ggggcatctg gtcggaagtc agttgttggc cgacataggc gagtccttgc gccgcacttt
     3901 ggttcagttt ggacgtaatt ataaaaagaa tttcgaatcg ttggtggctc gctacggcgg
     3961 ggacgagttt gttgtggttc ttatgggccc caacgttgac gattcactcg gattggccga
     4021 gaccattcga gcgcgtattg cctcaaaaaa aggtctctcc cgcccgatta ccgcaagtct
     4081 tgggcttgca tggaccggct ctggtgtgcg aagacgagat tatcgcgcct tgttaaaagc
     4141 ggctgacgaa gccatgtacg aggccaaacg tttgggccgc aaccgcgtgg tcgtcagcgg
     4201 caaaggagat cgtcatgagt tcacccaaaa gttcacttga aaccgctctc aaacagtatg
     4261 aaaaaacaaa gaaccaacac ctaagtgatt taaaagaatt ggtacgtata cccagcgtga
     4321 gttttgatgg gttcgatcct aaaaacgtgc agcgctcggc cgaggcggta gcggcacttc
     4381 taaaaaaacg cggtctcgaa aacgtagaga ttattaagtt ggccgacgac gttcacccgt
     4441 acgtgtacgc cgattggttg catgcaggac cgaacgcacc aacgttgctg ctttacgcgc
     4501 atcatgatgt gcagcctccg ggccgaatgg aaatatggaa atccgctccg tttgaaccgg
     4561 tggagcgaga tgggcgattg tatggacgcg ggactgccga cgacaaagcg ggtgtgattg
     4621 tgcattcggc tgcgatcgct tcctatcttg aaaccgctgg taaattgcca gtgaacgtaa
     4681 aactcgttat cgagggcgaa gaggaaatcg gttctacgca cctaaaagaa tttttggcga
     4741 agtataaaca gaaattaaaa gcagatgtaa tggtactcac cgatactcag aactacgata
     4801 ctgggttgcc ggcgcttact gtttcgttgc ggggactggt cgttttcgaa gtagaagtta
     4861 aagcgcacga acgctcaatg cattctggca tgtggggtgg tccggtggcc gaccccgtta
     4921 ttgcgctcag tcgcatgatc gccaccatga ccgacgctga agggcgattg actgtaccag
     4981 gaatttggga cgaagtacgc ccggtcacca aagaagagcg agcagaaatg gatcgattgc
     5041 cgtttacgat tcaggagttt cgctatcagg cgggcttgct accaagtacg cggttgcttc
     5101 caggtaaaga tggtactgta cacccgttgc tgcaaatgtg gcgcgagccg tcgctttcgg
     5161 tcaacgcggt tgaggcaagc tcacgaaagg cgcccgccaa tatcattaac aactcggcgt
     5221 gggctcagtt gggcgtgcgt atcgtggcgg acatggaccc cgtgcgaacg gcgcgtttgg
     5281 tagaggagca cctgaaacga gtggccccgt ggggagtaga agtttcagtt aaaaccgata
     5341 tcggcggaaa agcttggggt acgagggcta ccggccctgt gttcgacgct gcaatgcgat
     5401 cgcttgaaaa gggttacggc aagcgacccg tgcttatggg ttgcggcggc acgattccgt
     5461 ttgtagagcc gttttcagat gcgctcggtg gggcaccagc cattttgatt ggcgtcgaag
     5521 acccttacac tcaagcgcat tccgaaaacg aaagcatgcc actagcagat ttcgaggcgg
     5581 cggttcgcag cgcgatttat ttttatgacg aattgggcaa ccgctaacgg gcgcttgaat
     5641 agctagcccg cgggcttttg aaccactatg ctgcttcgac gtaatcgtcg gcgtgccaga
     5701 gttcgtagag cgtttttccg ttcggtgctt taaaccaaca gctggcgtgg tttggtggtt
     5761 cttgtcccag tagttccatt gaataagcat gtaatgtggt gacagtttct ttacccacct
     5821 tgagtttgta ctcgccatta ggctgcttca tgattttcgc ggtcttggaa gggtcggaaa
     5881 caaagctaat cgaatccccg tcttttaaat acccttcgtg aacgaggtgc gacaaatcga
     5941 atttcttttt ctttcccatt ttgatttccc ctttcgttgg atcacggact taaaagtatg
     6001 gccgctccgc cttcgcgttg agtggagcga tagaacgaac ttacgctcga gtcgtttcgc
     6061 ggcatattgg attgcgaaag acaatcggcg taagcggaat cagagatcga aacactgtgc
     6121 ccattggcgt cgcgacaa
//
LOCUS       DAOMXO010000042         6065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6886_length_6065_cov_5.112313, whole genome shotgun sequence.
ACCESSION   DAOMXO010000042 DAOMXO010000000
VERSION     DAOMXO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6886_length_6065_cov_5.112313"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLH57_01645"
     CDS             <1..192
                     /locus_tag="PLH57_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01343.1"
                     /translation="KFRDQEAADQVKARTLEVLRVKDPEFGDQTVRKIYEGILDAWMA
                     PQLAPGILPESLTAHENLK"
     gene            189..1292
                     /locus_tag="PLH57_01650"
     CDS             189..1292
                     /locus_tag="PLH57_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRK01344.1"
                     /translation="MSAFKNWIDRKLLTRMGEAIGDVYPTFDRKRLLAIAPKLKSLEL
                     KARVRLVRDCLHELLPSQASAAIDVLLKSTRGGLLDGFDLWPYTEFIQTYGLNEPQKS
                     LDALKELTVRFTGEFAVRPFLLRHPEITLAYLDRCTTSPNVHVRRWVSEGSRPRLPWG
                     ERLGPFIANPDPGLRLLEKLKYDSELYVRKSVANHLNDVSKDHPNRVLTLLAGWNKTV
                     PPIHRSKIAWVTRHALRTLIKNGNVNALALIGVRAEAAVKVAELRLNKKTFELGEVLE
                     FSFNLRSQAKIPQKLVIDYAVHHVKANGKTSPKVFKLKTFVIDPQESRELRKKHAIKA
                     ITTRRYYPGAHKIDLIINGQVRGEVRWNLVGRT"
     gene            complement(1289..2053)
                     /locus_tag="PLH57_01655"
     CDS             complement(1289..2053)
                     /locus_tag="PLH57_01655"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRK01345.1"
                     /translation="MIPRLGVNIDHVATLRQLRGTPYPDLLEAARLCERGGAEQITIH
                     LREDRRHIQDADLFALRKSVRVPLNLEMSLEPKMHKIARKAAPDWICLVPEKRQEVTT
                     EGGLDVVKQRRRIKNAIKLFAKSKTKISLFVEPEAAIMKLCKELGAHAVELHTGRYCL
                     AAQKGFGARSTKVEAAELNRIELAGHAAAQLGLHVNAGHGLDYQNIKPILAIRRENEH
                     PLIEEYNIGHVIVCRAAIVGIEQATREMANLVRSEL"
     gene            complement(2050..3315)
                     /locus_tag="PLH57_01660"
     CDS             complement(2050..3315)
                     /locus_tag="PLH57_01660"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HRK01346.1"
                     /translation="MKLPGVFVIPEGVQVQSVVIDDHLDGREVDLVSALGFGPKLAVV
                     SDVDTHRAMAEQVEYALSDKFKIQSVVLPHQPHADDVTLNKLLDVIPAGIDGVIAVGS
                     GTINDLCKMVAFRRGCPQAVFGTAPSMNGYTSINAAITVNGLKKSLHAAEPRGVFLDL
                     SVLAAAPKRLIRSGLGDSLCRPTAQADWLLAHSLFEQTPYNELPFQLLAGDEKDLFSH
                     SRALVGGDLYAMKHLARTLVLSGFGMTLCGGSYPASQGEHLISHYIDMVGGPGLPNTF
                     HGEQIGVTTLAMARIQEKILNSETPPTVRPTRVTRESVIDHFGDPLGHSCWLEFEHKY
                     LDSSKAAALNSKLQNHWPRIRDRIRAVTIPAATLKSVLVSAGAPTEPNDLGWSPALFD
                     NALKHAREIRNRYTFLDFAADTTQEADRV"
     gene            complement(3312..4850)
                     /locus_tag="PLH57_01665"
     CDS             complement(3312..4850)
                     /locus_tag="PLH57_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRK01347.1"
                     /translation="MLAAKTTLNSGARNTSSKSVRHVIGIDVGTQSLKAVVIDERMAI
                     VASASESYDVEFPQPGWAEQNPLVWESAISKVITAVLKQSGLQAENIAALGIASQLDG
                     CIPVDRNGRPLSNCLIWMDRRATTQSERVDPKLVRTKNGITADAGHMAAKISWFQENN
                     PQTSVHCFHQPVSYLVARLTGEHVMDHAIASTTMLYSLEKRDYDSDLLKAFSIDRETL
                     PRIDQAAARAGTLTLDAASWTGLSEGLPVAVGTGDDFSSVLGAGLTEPGQMICVLGTA
                     EVVGALDTAPKNDALGLVETHPLTKDIFFIENPGWLSGGSLTWFQEIFGIDRVEMLDT
                     LAAAAPAGSEGVIFLPALSGAMAPEWNANARGCFYGLTAAHHRGHMARAVLEGCAFAM
                     NDVLQRLRALHVSIDSILLTGGGAKSVLWAQIRADITGLPVRISEVKDTSPLGAALCA
                     AVASGLQPDLAAGSKLLRQVATEIKPNLALREVYDQAYSRYLSLFDALKPLFSPSSNE
                     ETHS"
     gene            complement(4837..5502)
                     /locus_tag="PLH57_01670"
     CDS             complement(4837..5502)
                     /locus_tag="PLH57_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01348.1"
                     /translation="MDFIFMLTRSDQTVSDCLDVLNDIESLQLKHLGFKDIGVPPTTL
                     LELNRRIKATGAISYMEVVSTSKEACLRSASVAREIGVDRLLGGTYVEEVLGILKGSA
                     TTYFPFPGRPFDHPTKLGGSPQIVEDDCRRFEQMGCAGVDLLAYRATEAAPLDLVRAA
                     RRALKGQLIVAGSVTTAQQIRDLKDAGADAFTIGSAVFDGSYSKLKGSLRSQLRDVLD
                     ACR"
     gene            5646..>6065
                     /locus_tag="PLH57_01675"
     CDS             5646..>6065
                     /locus_tag="PLH57_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRK01349.1"
                     /translation="MSAELKKTLGPVMLWGLGVGYVISGMYFGWNLGLPLGGPYGFLV
                     ATLLVTLMYITFVLSYAELACALPKAGGAFVYGNRAFGPGLGLLSGMAQCVEFVFALP
                     AVAAAIGAYLNLFFPQVPALAIAMGAYFFFTALNIYGV"
BASE COUNT         1470 a         1460 c         1597 g         1538 t
ORIGIN      
        1 aaatttcgcg atcaagaggc ggccgaccaa gtgaaagccc ggacgctgga ggttttgaga
       61 gtaaaagatc cagagtttgg cgatcagact gtgcgtaaaa tttatgaagg tattttagat
      121 gcatggatgg cgcctcagtt ggctccggga atattacctg aatctttgac cgcacacgaa
      181 aatctaaagt gagtgcattt aagaattgga tcgaccgaaa attacttact cgcatgggcg
      241 aagcgatcgg agatgtttac cctacttttg atcgaaagcg actcttggca attgcgccca
      301 aattgaaatc tttagagctg aaagcgcggg ttcgtcttgt gcgagattgc ttacatgaac
      361 tattgccttc ccaggccagc gccgccattg atgttctttt aaagtctact cgtggtggtc
      421 tattagatgg ctttgatctt tggccctaca ccgagtttat tcagacctat gggttgaacg
      481 aacctcagaa atcgctcgat gcgctcaaag aattaacggt gcgctttact ggggaatttg
      541 cggtgcgtcc gtttttgctt cgacatccgg aaattacttt agcttacctg gaccggtgca
      601 ctaccagtcc aaatgtacat gttcgaaggt gggtgtcgga aggttcgcgc ccgcggctgc
      661 cgtggggtga acggctcgga ccgtttatcg ccaatccgga cccgggactg cgtttgttag
      721 aaaaactcaa atacgactca gaactctatg tgcgcaaaag cgttgccaac catctcaacg
      781 atgtttcgaa agatcaccca aaccgagtac tcactttact ggcgggttgg aataaaacag
      841 ttccccctat tcatcgttcg aaaattgcct gggtcactcg ccacgctctt cgtactctta
      901 ttaaaaacgg aaatgtgaat gctctagcgt tgatcggtgt acgggccgaa gctgcggtaa
      961 aagtcgcaga gcttcgattg aataagaaaa cctttgaact aggtgaagtg ctagagttca
     1021 gttttaattt gcgatctcaa gcgaagattc cacaaaaact cgtgatcgac tacgcagtcc
     1081 accatgtaaa ggcaaacggc aagactagtc ccaaagtatt taagttaaaa actttcgtta
     1141 tcgaccctca ggaatcgcga gagctgcgaa aaaaacacgc aataaaagca atcaccacga
     1201 ggcgttatta tcctggcgct cataaaatcg acttgatcat caacggacaa gtgcgaggtg
     1261 aagttcgttg gaacttagtg ggtcggactt aaagttcgct tcgtacgagg ttcgccattt
     1321 cgcgggtcgc ctgttcgata cccacgatcg ctgctctgca aactatgacg tgaccgatat
     1381 tgtattcctc aatcaacggg tgttcgttct cgcgccgaat ggctaatata ggtttgatgt
     1441 tttgataatc aagcccgtgg cccgcgttta cgtgtaaacc aagttgagcg gctgcgtgac
     1501 cagcgagttc aatacgattg agctcggcag cttcaacttt agtggaacga gctccgaaac
     1561 ccttttgagc ggcaagacag tatcgtccgg tatgcagttc gaccgcgtgc gctcccaact
     1621 ctttgcagag cttcatgatt gcggcttctg gttcgacgaa aagactaatt ttggttttgg
     1681 atttggcaaa aagtttaatg gcgtttttga tgcgacgacg ttgtttgact acgtcgagcc
     1741 cgccttcggt agtaacttct tgacgttttt cagggaccaa gcagatccag tctggggcag
     1801 ccttgcgggc tattttatgc attttgggtt cgagcgacat ttcgagattg agaggtacgc
     1861 gcacgctttt gcggagggca aacaggtcag catcttgaat gtgacggcga tcttcacgca
     1921 ggtgaatcgt aatttgttcg gctccaccgc gttcgcaaag tctcgctgct tcgagcaggt
     1981 ctgggtaagg cgtgccgcga agctgtcgca gagtggccac gtgatcgatg tttactccca
     2041 aacgaggtat catacgcgat cagcctcctg ggttgtgtcg gccgcgaaat caagaaaagt
     2101 gtagcgattg cgaatctcgc gggcatgctt gagcgcgttg tcaaacagcg cgggcgacca
     2161 accaagatca ttcggctcgg tcggagcacc ggcactcact aaaacagact ttagagtggc
     2221 tgcaggaata gtaactgctc ggatgcggtc gcgaatgcgt ggccagtggt tctgcagctt
     2281 gctattcagt gcggcggcct tagagctatc gagatactta tgctcaaact ccaaccaaca
     2341 cgaatggccg agcggatccc caaagtggtc gatgacggat tcccgcgtaa cgcgagtcgg
     2401 tcgtacagtg ggcggggttt cagagttgag aattttttct tgaattcggg ccattgccag
     2461 cgtggtgacg ccgatctgtt caccgtggaa agtgttcggt aggccggggc cgccgaccat
     2521 gtcgatgtag tgactgatta aatgttcgcc ttgactggcg gggtaactgc caccacaaag
     2581 agtcattccg aatccagata acactagagt gcgtgccagg tgcttcatgg cgtaaagatc
     2641 gccgccaact agagctcgtg aatgtgaaaa aagatctttt tcatcaccag cgagcaattg
     2701 aaacggtagt tcattgtaag gggtttgttc aaataaggaa tgagcaagca gccagtccgc
     2761 ttgggccgtg ggtcggcaaa ggctatcgcc aagtccagag cgaatgagtc gtttaggggc
     2821 tgcagccaac acgcttaagt ctaaaaatac accgcgcggt tcggcggcat gcagcgattt
     2881 ttttaaacca tttacagtga tcgctgcatt gatggatgtg tagccattca tagaaggagc
     2941 ggtgccaaac actgcctgcg gacaaccgcg acgaaatgcg accattttac aaagatcgtt
     3001 tatggtgccg gaccctacag caattacgcc gtcgattccg gcgggaatca cgtcgagtaa
     3061 tttatttaac gttacgtcgt cggcgtgagg ctgatgtgga agtacaacac tttggatttt
     3121 aaatttatcg ctgagcgcgt attcaacttg ctcagccatg gcgcgatgag tgtcgacgtc
     3181 actaaccact gcgagtttcg gcccaaaacc gagagccgaa acgagatcca cttctcgacc
     3241 gtcgaggtgg tcgtcaatga cgaccgattg tacttgaacg ccttcaggga tcacaaagac
     3301 accaggaagt ttcatgagtg agtctcctcg ttggaagagg gcgaaaataa tggtttcagt
     3361 gcgtcgaaca aactaaggta acgtgagtac gcctgatcgt agacctcgcg caaggccaaa
     3421 ttgggtttga tctcggtcgc aacttgtctg agcaatttgg agcccgccgc cagatctggt
     3481 tgtagtccac ttgccactgc tgcacacagt gcggctccta atggagaagt gtctttcact
     3541 tcagaaattc gcacaggaag tccagtgata tcggcccgaa tctgggccca caatacactt
     3601 tttgctccgc cacctgtgag taatatagaa tcgattgaaa cgtgaagtgc tctcaggcgc
     3661 tgcaacacgt cgttcatggc gaaggcgcac ccttcgagca cggctcgtgc catgtgacca
     3721 cgatgatggg ccgctgtaag gccgtaaaaa caacctcgtg catttgcgtt ccattcggga
     3781 gccattgctc cactaagagc aggtagaaat ataactccct ctgatcccgc tggcgcggcg
     3841 gcggccaatg tgtcgagcat ttctacgcga tcgattccga agatttcctg aaaccaagtg
     3901 agagaaccgc cagagagcca gccggggttt tcgataaaaa agatatcttt ggtgagtggg
     3961 tgagtttcca ctaatccaag ggcatcgttc ttaggagcgg tgtcaagcgc gccgaccact
     4021 tccgcagtgc cgagcacgca gatcatttgc ccgggttctg tgagtcccgc acctagaacg
     4081 ctcgagaagt catcgcccgt accgacagcc acaggcaggc cttccgaaag tccggtccaa
     4141 gaggctgcat cgagagtcaa agtgcctgcc ctagcggcag cctgatcaat gcgtggcaat
     4201 gtttcgcgat caatcgaaaa agctttaagc aggtctgaat cataatcgcg tttttcgagt
     4261 gaatacaaca tggtggtgga agcaatggcg tgatccatga cgtgttcccc ggtgagtcgt
     4321 gccaccaagt acgaaaccgg ttgatgaaaa cagtgtaccg atgtttgtgg gttattttcc
     4381 tgaaaccagg aaatcttcgc agccatgtgg cctgcgtcgg cagtgatacc gtttttggtt
     4441 cgcactagtt tggggtcgac acgctcgctc tgggtagttg cacgtcggtc catccaaatt
     4501 aagcaatttg aaagcgggcg cccgttgcgg tctaccggaa tgcagccatc cagctggctc
     4561 gctattccga gtgccgcaat attttcggct tgtagaccgg attgttttaa aaccgcagta
     4621 atgactttgc tgatagcgga ttcccagact agaggatttt gttccgccca acctggttgc
     4681 ggaaactcaa catcgtaaga ttcgctagcg ctcgccacga tggccattcg ttcgtcaatc
     4741 acaacagcct ttaagctttg agtgcccaca tcgatgccaa tgacatgacg aacagatttt
     4801 gaacttgtat tccgagcgcc ggaattcaac gtagttttag cggcaagcat caagtacgtc
     4861 cctcaactgc gaacggagtg atcctttgag ctttgaatag gagccgtcaa aaaccgcaga
     4921 cccaatcgta aatgcgtctg cacctgcgtc tttgagatcg cgaatttgtt gtgccgtagt
     4981 cacgctgcca gcgacgataa gttgcccttt aagtgcacga cgcgcagctc gtacgagatc
     5041 aagcggtgct gcttcggtgg cccggtaggc gagtaagtcc acgccggcac aacccatttg
     5101 ttcgaagcgt cgacaatcgt cttcaacgat ttggggggat ccacccagtt tggttgggtg
     5161 atcaaacggt cgccctggaa atggaaaata agtggtcgcg gaccctttaa gaatgccaag
     5221 aacttcttct acataggtgc cgcctaataa acgatcgacg ccaatttcgc gagctacact
     5281 tgcagagcgc aagcaagctt ctttgcttgt gcttacgact tccatgtaac taatagctcc
     5341 ggtagctttg attcggcgat taagttctaa aagggtggtg ggcggaacgc cgatgtcttt
     5401 aaatcctaag tgtttcagct gtagcgactc gatatcgttt aatacgtcta aacagtctga
     5461 tacagtttga tccgatcgcg tgagcatgaa gataaagtcc atgatgttcc ccttgaattt
     5521 aattaaaaaa ttcccctaaa tttccagccc aagatttccc acagcgctcg ggcgatggca
     5581 atattctttg cattttcgag tacgcaaaag tagtgaataa atctcaacat tcaggggggc
     5641 gttccatgtc tgcagaactc aagaaaactc taggtccagt catgctctgg ggcctaggtg
     5701 tcggttacgt gatttcaggc atgtacttcg gttggaatct aggactaccg cttggcgggc
     5761 cttatgggtt tctagttgcg acactacttg tcaccttaat gtatattacc tttgttttga
     5821 gttacgccga acttgcatgt gcacttccga aagcgggtgg ggcttttgtt tacggcaacc
     5881 gagcatttgg tcccggctta ggtttgctct caggtatggc tcagtgcgta gaattcgtat
     5941 ttgcgcttcc cgccgtagca gccgcgatcg gcgcgtattt gaatttgttt tttccgcaag
     6001 tgccggcact ggcgattgcg atgggggcct atttcttttt cactgcctta aatatttatg
     6061 gagtt
//
LOCUS       DAOMXO010000043         6018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6965_length_6018_cov_4.978702, whole genome shotgun sequence.
ACCESSION   DAOMXO010000043 DAOMXO010000000
VERSION     DAOMXO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6965_length_6018_cov_4.978702"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..715)
                     /locus_tag="PLH57_01680"
     CDS             complement(173..715)
                     /locus_tag="PLH57_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01350.1"
                     /translation="MSKGWLVVWIGFTGTFAAVSPARANLNAGIEALTMGSALGSEVR
                     DRIEVVTGTTSDCVYDMESGRITVSETIAASETEVVARCLAPAVAQMKYESIEDQWLE
                     HSGVRQAWVLDRGSVRTKELSNKVVTEPTARATWVVLASREMLLQGTKEKKGDPSRGI
                     ASIPIAKSDSKIRELLGLVN"
     gene            complement(734..1255)
                     /locus_tag="PLH57_01685"
     CDS             complement(734..1255)
                     /locus_tag="PLH57_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01351.1"
                     /translation="MIHMWKWALCLGIVSMMSMASYGRAVKNKRGSKVETPEFRAESI
                     VPGETPESKLLVLVGQPMRVLRERGRVLYFYDVGLRGASMTATVSVRKGVVEWMTYFC
                     DEPVKEVLSKYEGVKEGTVALSGVDKRSLRGGLRQLTVDEVGRGFVYSPRAEKVRVCL
                     RWPTGKPFSEVGF"
     gene            1450..1665
                     /locus_tag="PLH57_01690"
     CDS             1450..1665
                     /locus_tag="PLH57_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01352.1"
                     /translation="MAHSSPVTGIIEENLVIIDFGKHEGKTVSIIAEEDPAFYQVLVL
                     QKDNFAIRRHRDKSFRLYRNPLFQIEV"
     gene            complement(1690..4311)
                     /locus_tag="PLH57_01695"
     CDS             complement(1690..4311)
                     /locus_tag="PLH57_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01353.1"
                     /translation="MKSVYFLALGIGVAPLALAQTAPAPNPGQQASIDQAAVTAGELS
                     VCEGALHQIGPSQATIQKAGATMMGSSQSFGGANFNACAMQGLQQTTGSAANLTPDQM
                     KAKLVVDSTLNAAQVANDFFGAKNSGAVCSLTADPYEDAHVANKYLGASCDSFRGPAT
                     RDGKEGTYSEAQLMDAIRAVSEEQGKARCEIKRVAAARQSLECFQSNYEKVRASMLHF
                     RDDLDDKRECVGSYITEVEGLAEKQKLRATELDERVRQFSQATARAQEMLDKIQEKEP
                     QLNEAIPTLRQERARLYSALRSDVISKFHDCVNGDAAYNSPLRRSRCLERSPSGDGRK
                     GKFERVETRTCLVNRYNEYSGGNSKENIDKDQAGDKSSLFSLDLENIDSSLMGELELN
                     KPNLSDSVDVLHAIEDASGALQKQDARLRRLEEEYGSFGKAYASQFRWCMANVKNASQ
                     KEYNSETGTLAARRMDYESKVREVASDFRATAKAMRDTVKSFEKTLGVDLTLHLNSHH
                     CGSAVQNPGKSEGPATLDQRKECINHLTDLVETVKSGSNLPDSNGGLIMPIPGVIDTA
                     KSPECRGLGAGGCVCTGLEDCADKYGKVSSAITQNVELMKGKGHYVDPSCTTMLCVKK
                     PGAELAKEKVNASISKAIQAQGRAYAGIAQGNQAALAQLNALLSKIPGAGRLGGFDPT
                     AQSAFSCAEKDEKGICAVPDDLSAAVGAIAGMPVLSNEAFETRISALANADSKPKEDQ
                     RAIGEVLGRLATLKVSCANQKKKEGVVDELDALRGDLAEAGNACEEYAEMHSPDLARI
                     ELAEIFENTLKGVHDICDKNPGALQVARKDCAQFKKEIGNTKRECGRYITSREEARRM
                     GYGGVRNLADDNSKSAY"
     gene            complement(4432..5790)
                     /locus_tag="PLH57_01700"
     CDS             complement(4432..5790)
                     /locus_tag="PLH57_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRK01354.1"
                     /translation="MSAHFGYGGADYLAALTWARTDALLRAFDRAVDATVNAIAQNLL
                     AAQQGTIKEKTVAGIVPSSSTKTPISIDAGSRDIKMSQPVAKYRDGFWRKLWLSIKGT
                     ATLPYRIWRYYQYDQDYRGADSGRIELKRASSVVLKDRNRFWAHHRLRLPEAWARHGV
                     GDRRVIVAVLDSGMDYNHSELNRTLYWDGLRESAGWDFISGDARAFDDHAHGTEVASI
                     VGGGWSKSMGVAPGVTLLPVKVFSPYGQTSSGSIVAAFEYAIASGARIIVCGWATPVR
                     SAALRSAIEVAKAAGVLVVAAAGDDGTNLDDEPRYPAAYSREFDNVVSVAAYGLRSIP
                     KTNISEVVLWRSKNQKIASSYGPRTVDLAAPGEDLFVARPRGKTRTQSHSGLAAGVVA
                     GVAALATSVCPTVSASELKVALLDDASPSEPDLDWQVSGARALLDLRFLNRLAGACKK
                     GK"
     gene            complement(5839..>6018)
                     /locus_tag="PLH57_01705"
     CDS             complement(5839..>6018)
                     /locus_tag="PLH57_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01355.1"
                     /translation="PLTREPGWFGPTFRDTGGLRTGLEIGHEINFEVVLAGARVKRLP
                     YAAHFGTDLVFSTDK"
BASE COUNT         1349 a         1722 c         1427 g         1520 t
ORIGIN      
        1 gtatcaattc cctggccgga gctcgaatcg gaagcacttt taaatttgga aaactcgatc
       61 ttgagtcggc tttcggtagc ggcaccggtt cgaaccaaac cgacaacatg caacggatag
      121 gttttgactc aaatactttt gtgttggtga gcgcgactta tcacttagat cgctagttaa
      181 cgagtccgag caactctcgg atcttggagt cggatttagc aattggaata gatgcgattc
      241 ctcttgaggg atcgcctttt ttttcttttg ttccttgcag aagcatttcc cggctcgcaa
      301 gcaccaccca cgtggcgcga gcagtgggct cggtcacaac tttatttgac agttccttcg
      361 tacgaacgct tccacgatcc agtacccagg cttgacgcac gcccgaatgt tccaaccact
      421 gatcttcgat tgattcgtat ttcatttggg caacagctgg ggccaagcac cgcgccacaa
      481 cttcggtctc gctcgcggcg atggtttctg aaacggtgat tcgcccggac tccatgtcgt
      541 aaacgcaatc ggaagtggtc cccgtaacca cctcgatgcg atctcgcacc tcacttccaa
      601 gcgctgatcc catggtgagg gcttctattc cagcgttcaa gttcgcgcgg gctgggctaa
      661 ccgccgcgaa tgttccagta aatccaatcc aaaccacgag ccatcctttg ctcatatttt
      721 tttctcctaa aacttaaaag cctacctcag aaaagggttt accagtgggc caacgcaaac
      781 acactcgcac cttttcggcg cgaggtgagt aaacaaaacc tcgtcccact tcatctaccg
      841 tcagttgtct caaaccgcct cttaacgagc gtttatcaac cccggacaac gccacggtgc
      901 cctctttgac gccttcatat ttgctgagca cttctttaac tggctcgtca cagaaataag
      961 tcatccattc gactacgccc tttcgtaccg atacggtcgc agtcatcgac gcccctcgaa
     1021 ggccgacgtc ataaaaataa agcactcgcc cacgttcccg cagcacgcgc atgggttgcc
     1081 ccaccaggac aagcagtttt gattcgggtg tttcgccggg aacaatcgat tcagcacgga
     1141 actccggtgt ttcaaccttt gaaccgcgct tattttttac ggctctgccg taacttgcca
     1201 tgctcatcat actcactatt cccaaacaca atgcccattt ccacatgtga atcatccttc
     1261 gttgcttcag cactgacgca cgattagttt aacgaaaagt gaggggtcgg tattgatttt
     1321 tttttcctga tcgatacgat ttggctgagg agttttttct cggttttttt tgccgggttc
     1381 cttggcatca atcgctgtcg cagtacatga agtacacact caaacgaacc acggaggatt
     1441 cgcacgcgca tggctcattc cagcccggtt acgggaatca tcgaagaaaa cttagtcatc
     1501 attgattttg gaaagcacga gggaaaaacg gtttcaatca tcgccgaaga agacccggca
     1561 ttttatcagg tactcgtttt gcaaaaagac aattttgcaa ttcgcaggca tcgcgacaaa
     1621 agctttcggt tgtaccgaaa tcccttattt cagattgaag tctgaaaaac agcgatattc
     1681 gactgccctt tagtaggcgc ttttactgtt gtcgtcggct agattgcgaa ctccgccata
     1741 acccatgcgt cgggcttctt cgcgcgacgt gatataacgc ccgcattcgc gcttcgtatt
     1801 gcctatttct tttttaaact gcgcgcagtc tttgcgagcg acttgtagcg ctccaggatt
     1861 tttatcacaa atgtcgtgaa cgccctttaa cgtattttcg aaaatctccg ccaactcaat
     1921 gcgcgcgaga tcgggagagt gcatttccgc atactcctcg caagcattac ccgcctcggc
     1981 taaatcacct cgaagtgcgt cgagttcgtc gaccacccct tcttttttct tttggttcgc
     2041 gcaactcact tttaacgtcg ccaggcggcc gagaacttcc ccaatggcac gctgatcttc
     2101 tttaggttta ctatccgcat tggctagcgc ggaaatacgg gtctcaaaag cctcattgct
     2161 aagtaccggc atgccggcga tcgcacccac tgcggcgctg aggtcgtctg gtaccgcgca
     2221 gatgcctttt tcatcttttt ctgcacaaga aaacgcgctt tgggcggtcg gatcaaagcc
     2281 gcctaagcgg ccagctcccg gaattttcga taatagcgcg ttcaattgag ccagcgcagc
     2341 ttgattgccc tgagcaattc ccgcgtaggc cctgccttgg gcttgaatgg ctttacttat
     2401 agacgcattc actttttctt tggccaattc cgctccgggt tttttcacgc agagcatcgt
     2461 agtacacgac gggtcaacgt agtggccttt gcccttcatg agttcgacgt tctgggtaat
     2521 cgccgaactt actttgccgt atttatccgc gcagtcctcg agtccagtgc acacgcaccc
     2581 acccgcgcct aaaccgcggc actccggcga ctttgcagtg tcgatcacgc ccggaattgg
     2641 catgatcaga ccaccattcg aatcaggcag attactgcct gacttcacgg tttcgactag
     2701 atcagtgagg tgattgatac attctttacg ttgatcaagg gtagcgggac cttcggattt
     2761 tcctgggttt tgcaccgctg atccgcagtg gtgactattt aagtggagcg taagatcaac
     2821 acctaaggtt ttctcaaagc ttttcacagt gtcgcgcatc gctttggcag tggctcgaaa
     2881 atcagaagcc acttcgcgta cttttgattc atagtccatt cgacgggcag cgagcgtacc
     2941 ggtttcgcta ttgtattcct tttgactggc atttttcaca ttggccatgc accaacgaaa
     3001 ttgactggcg taggctttgc cgaaagatcc gtattcttcc tccagccgtc taagacgagc
     3061 gtcttgcttt tgcagtgcgc ccgaggcgtc ttcgatcgca tgcaacacgt cgactgaatc
     3121 cgaaaggttc ggtttattca actcgagttc acccattaac gacgaatcaa tattctctag
     3181 atcgagacta aagagcgatg acttatcgcc agcctgatct ttgtcgatgt tctctttgga
     3241 attcccacct gaatattcat tgtacctatt caccagacag gttcgcgttt caactcgttc
     3301 gaacttgccc tttctgccgt cgccactggg gctgcgctca agacagcggc tgcgacgtag
     3361 ggggctgttg taggcagcgt cgccgttcac acaatcatga aatttgctga tcacgtcgga
     3421 acgcaacgcc gaatacaacc tagcccgttc ttggcgcaaa gtcggaatgg cctcgtttaa
     3481 ctgtggctct ttttcttgga ttttatccaa catttcctgg gcccgggcgg tagcctgtga
     3541 aaactgtcgt acacgttcgt cgagctcggt tgcccgcagt ttctgttttt cggccaaacc
     3601 ttcgacttca gtaatgtaag aacctacgca ctcgcgctta tcatcgagat cgtctcgaaa
     3661 gtgcaacatt gaagcccgca ctttttcata gttcgattga aaacactcga gcgactggcg
     3721 cgcggccgcc actcgtttta tctcgcagcg agctttgccc tgttcttcag aaaccgctcg
     3781 aatcgcatcc atcaactgag cttcactgta tgtgccctct ttaccgtctc tggtagccgg
     3841 acctctaaaa ctgtcgcagc ttgctcctag gtatttgttc gcaacatggg cgtcttcgta
     3901 gggatcggcc gtgagcgaac aaacagcgcc agagttttta gccccgaaaa aatcgtttgc
     3961 cacctgcgcg gcgtttaagg tgctatcgac cacgagtttg gctttcatct gatcaggcgt
     4021 cagattggca gcgctaccag tggtttgttg aagtccctgc atggcacaag cattgaaatt
     4081 ggcaccacca aacgactgag acgaacccat catcgtggcg ccggcctttt gaattgttgc
     4141 ctgggaaggg ccgatctgat gcagcgcgcc ttcgcaaact gacagctcac ccgccgtcac
     4201 ggcggcctga tcaatactag cttgctgtcc tgggtttgga gccggcgccg tttgcgccaa
     4261 ggccaacggg gccacgccaa tgcccagagc cagaaaataa acacttttca ttcaagatcc
     4321 cccacaaaaa ccggtccact catcattata gcgagtttga aaatcaacac aatatcagat
     4381 taaaataatc atgaactact cgctccaata cgctgttttt attgtgtttt tttattttcc
     4441 ctttttacaa gccccggcaa ggcggttcaa aaatcgcaga tctagcagag cccgagcccc
     4501 gctcacttgc cagtcgagat cgggctccga cgggctggcg tcgtcgagca aggcgacctt
     4561 tagttcggaa gcactcaccg ttgggcacac gctggtagca agtgctgcca ctcctgccac
     4621 cacacccgca gccaaacctg agtgactttg agtccgggtt ttccctcgag ggcgggcgac
     4681 aaagagatct tccccggggg ctgctagatc gacggttcga ggcccgtagc ttgaggcaat
     4741 tttttgattt ttacttcgcc acaagaccac ttccgaaata ttggttttag gaattgagcg
     4801 cagcccgtag gccgctacac tcacgacgtt gtcaaactct ctcgagtacg cagcggggta
     4861 acgtggttca tcgtctaagt tcgtaccgtc gtcgccagcc gcagcgacga ccaacacgcc
     4921 agcagctttc gcaacttcaa tggcacttcg caacgccgca gaacgtacgg gcgtagccca
     4981 accacataca atgatgcggg caccgctggc gatcgcatat tcgaaggccg ccacgatgga
     5041 gccagaactg gtctgcccat agggactgaa cacctttaca ggcagcagcg tcactccggg
     5101 agctaccccc atggacttcg accacccacc gcccactatc gacgccactt cagtgccatg
     5161 ggcatggtcg tcgaacgcac gagcatcgcc cgagatgaag tcccaacccg cgctctctcg
     5221 cagtccgtcc caataaagcg ttcggtttaa ttcagaatga ttgtagtcca ttccactgtc
     5281 gagcacggcg acgatcacgc gtcgatcccc aaccccatgg cgcgcccacg cttcgggtaa
     5341 ccgcaaccgg tggtgggccc agaatcgatt ccgatctttt aataccactg aactcgctcg
     5401 cttgagttcg atgcgacccg agtccgcacc acgataatct tggtcgtact ggtagtaccg
     5461 ccagatacga tacggcagag tggcggttcc cttgatcgac aaccataatt tgcgccaaaa
     5521 cccgtcgcga tacttggcca ctggttggga catcttaatg tcccgcgagc ccgcatcaat
     5581 cgatattggc gtcttggtgc ttgagcttgg cacaataccc gcaacagttt tttcttttat
     5641 ggttccttgt tgagcagcaa gcaggttttg cgcaattgcg ttgaccgtgg cgtcgacagc
     5701 gcgatcaaac gctcgcaaaa gggcatcggt gcgagcccag gtgagcgctg ctaaatagtc
     5761 cgctccccca taaccaaagt gagccgacac gtcgaagtaa aaacccgaag ttctcgcatt
     5821 gatggcctgt tcgactactc atttgtcggt actaaaaacc agatccgtac caaagtgcgc
     5881 cgcgtacggc aggcgtttta ctctcgcgcc ggcaagcaca acttcgaaat ttatctcgtg
     5941 gccgatttca agcccggtac gcaggccgcc cgtatcgcga aaagtaggac caaaccaacc
     6001 tggctcacgg gtgagcgg
//
LOCUS       DAOMXO010000044         6017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_6967_length_6017_cov_4.892318, whole genome shotgun sequence.
ACCESSION   DAOMXO010000044 DAOMXO010000000
VERSION     DAOMXO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6967_length_6017_cov_4.892318"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..963
                     /gene="mazG"
                     /locus_tag="PLH57_01710"
     CDS             1..963
                     /gene="mazG"
                     /locus_tag="PLH57_01710"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRK01356.1"
                     /translation="MTGQEFMLKMLHDKKMKDTRTTIFEGIDAQRTAMIEAMTRQLEL
                     AIGLVTRLRGPNGCPWDREQNPLSLRQYLIEEAYETLEVIDRYKPNADSKKIAAETAK
                     SEWVKVDGTFEKGDQVKLREELGDLLLQIILHAQLAWERGDFHLGDVGEFMAKKLVSR
                     HPHVFGDQVAENSDAVLANWEVLKKKEGRKSATEGLPKHLPSLQRAARLGDKVSRLGF
                     DWKDWTGAWAKVKEEVSELELAMQGKVNSEIEHEIGDLFFSLCNLARHLKIAPEDAHR
                     KAIGRFEDRFMKVEKLCEERGIQMEKATLEELDRLWDEVKMSEG"
     gene            complement(1035..2180)
                     /locus_tag="PLH57_01715"
     CDS             complement(1035..2180)
                     /locus_tag="PLH57_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01357.1"
                     /translation="MKHSQKITILACAVAALATTPAFGSLNEIMKTYRGLRSLGMGGV
                     AFTTGMYDEALFANPAMASEHPESRIEILSPTFEANTNLISNFSAVNDIKGSSGASAI
                     SKIADEGIAGENMHFRFTGLFGYYSPKLFGENTGFSFGLLTNLQANIMLRADADVDSQ
                     AYLDAGPNFGVSHLLMNGKLALGANLRAIYRLASNKTIRATEFLGGTKLSLSSIGGQG
                     MGIDGDLGALYKMMDVHSFFKNLWFALSLNNVAQSTYRVAAKDLVSSVKTSPPRNNRT
                     INTGVRTDLPDAWILSGNIFAIEVQDIGSLIYHSSFWKRLHIGGETKLTRVVSFRGGL
                     NQGYLAAGFGFDLPVLNVEVATYGEEMGTNTGIHEDRRIALRLGVEI"
     gene            complement(2238..2993)
                     /locus_tag="PLH57_01720"
     CDS             complement(2238..2993)
                     /locus_tag="PLH57_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01358.1"
                     /translation="MSNRTFSILAVLFVTISSFSMMSCNATGGIDTPETATEKLSEVK
                     ALLDEAKCEDAIQKLNDINQPDAEIARLRGWANLCRAGATIGRVGKSLYSFDTTLNNL
                     TVVGRLANEMVPTNDTKLSYIDEAINAFGQMGTSTDRSLVIAIAQMVKAAGYIAKYST
                     NGQRVFKTDIAIESCQQVSCSAATDITCPARMSNTDANVVGNALIAANNEIGSNSTLG
                     NLGTLASQLGGIASASTGAINRCYIISQMLPAE"
     gene            3049..3579
                     /gene="ybeY"
                     /locus_tag="PLH57_01725"
     CDS             3049..3579
                     /gene="ybeY"
                     /locus_tag="PLH57_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRK01359.1"
                     /translation="MKKPKKKSNKLDVVFLHPESFGRTKAARALKKSLVQMLNACRDL
                     KLISSGTTVVSISLVDDREMKKLNRAYRKKNALTDVLSFEQPIGDLSENPKGVRFLGD
                     IVFCRSVVRTQARTYGHSVEIELKILLAHGLLHLLGMDHERSSRALKKMLGFERQVLL
                     AAGVRQSPGLLDRAKL"
     gene            3589..4738
                     /gene="prfB"
                     /locus_tag="PLH57_01730"
     CDS             join(3589..3672,3674..4738)
                     /gene="prfB"
                     /locus_tag="PLH57_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157679995.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRK01360.1"
                     /translation="MSQIGQSSEIREGLKAIGEKLGFLRGSLDIANKERRLNEIADLS
                     TQAEFWADNRKAQSILKEKGRLENELGTFKKSYRDYEDLNALFELAEESGDESLYADA
                     RAQLTLLFGSVENIELQKMLSGEQDGLNAIVSINAGAGGTESQDWAQMLLRMYLRWAQ
                     QSGYKTEVVDIIEGEQAGIKSATVIISGVNAFGFLKSEIGIHRLVRISPFDSNARRHP
                     SFASVFVTPEVDDNFEVEIRDVDLRVDVYRAGGKGGQGVNTTDSAVRLTHFPTGIVVQ
                     CQNERSQIKNKAMAMKILKSRIYERHMEEERKKAAEVEAGKKDIAWGSQIRSYVLHPY
                     KMVKDHRTGFVSSQAEKVLDGDLDGHIKSYLRCSLTGEWARGGDSDID"
     gene            4781..5743
                     /locus_tag="PLH57_01735"
     CDS             4781..5743
                     /locus_tag="PLH57_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRK01361.1"
                     /translation="MSIGREIYVGTVGYNYRDWIGSFYPKGASSRRQLQLYSKQFNVC
                     ELTQFTYQMPEIDRMQNFVEGLGSSRTKFFVRIHNALTHGTDIGLALTVARHFRRALE
                     PLVETGRLAGFVATFPYAFKNSDASRMYLEQLAISLACPDLPLFVDFRHSTWVTPKSF
                     QWMAKRNLSFVCVDEPELPGLMPPLVTATQDSVLIRLHGRNATGWWSGNLVTRYDYLY
                     SKDELSGLLTRYLPLLEQGKQAHFLFANHWQAQSIRNGLEWRSMLAEYEMGESPTRAG
                     IADEDVLRAPVALPLEDVKSRVSLALSDLQISMDLPPSVDPESG"
     gene            5818..5894
                     /locus_tag="PLH57_01740"
     tRNA            5818..5894
                     /locus_tag="PLH57_01740"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5852..5854,aa:Asp,seq:gtc)
BASE COUNT         1575 a         1361 c         1585 g         1496 t
ORIGIN      
        1 gtgacaggac aagaatttat gctaaaaatg ctgcatgaca aaaaaatgaa agacactcgt
       61 acaacgatct ttgagggaat tgacgcacag cgaacggcaa tgattgaagc catgacgcgt
      121 cagttggaat tagccatcgg gcttgtgact cgtttgcgtg ggcccaatgg ttgcccttgg
      181 gatcgagaac aaaatccact ctcgctacgt cagtatctga tcgaagaggc ttacgaaacg
      241 cttgaggtga tcgatcgcta caaaccaaac gccgattcaa agaaaattgc tgctgaaacg
      301 gctaagagcg agtgggtgaa agtcgacggc acctttgaaa agggtgacca ggttaagttg
      361 cgcgaggagt tgggagattt gttgctacaa atcattttgc acgcccagct tgcctgggag
      421 cgaggggact ttcacttggg cgacgtcggt gagttcatgg ccaagaaatt ggtatcgcgc
      481 cacccgcacg tgttcggaga tcaggttgcc gaaaactctg atgcggtatt agcgaactgg
      541 gaagttctca agaaaaaaga agggcgtaaa agtgcgaccg aaggtttgcc gaaacatctg
      601 ccgtcgcttc agcgcgctgc ccgcttggga gataaagtga gtcgacttgg attcgactgg
      661 aaagattgga ccggggcttg ggcaaaggta aaagaggaag tgtctgaact cgagctagcc
      721 atgcaaggca aggtgaattc ggaaattgaa cacgaaatcg gcgatctgtt cttttcgctt
      781 tgcaatctgg cgcgacacct aaaaatagcc ccggaagatg cccatcgtaa ggcaataggg
      841 cgcttcgaag atcgatttat gaaggtcgaa aaactttgtg aagagcgagg aattcaaatg
      901 gaaaaggcta cgctcgagga attagaccgc ctgtgggatg aagtaaaaat gagtgaaggg
      961 taaaaaaaaa gcccggttgc gaagcgccga ctctaaaaaa gagacgcgct tcacaaccgg
     1021 gctaggaaag tcggctagat ttccacccca agtcggagtg caatacgacg atcttcatga
     1081 ataccggtgt tggtacccat ttcttcgccg taagtagcaa cttcgacatt gagaaccggc
     1141 aggtcaaaac cgaagcctgc ggccaagtaa ccttgattta aaccacctcg gaacgaaacc
     1201 acccgagtaa gtttggtttc gccaccaatg tggaggcgtt tccaaaacga agagtgataa
     1261 atcaacgagc cgatatcttg aacttcgatc gcaaagatgt tgccagatag aatccaagca
     1321 tcaggcaggt cggtgcgaac gccggtgttg atggttcgat tattgcgcgg aggactggtt
     1381 ttaactgagc tgactaggtc cttcgcagcg acacggtaag tgctctgtgc cacattgttc
     1441 aacgacaaag cgaaccagag gtttttgaag aaactgtgca cgtccatcat tttgtaaagg
     1501 gcacccaagt cgccgtcgat acccataccc tggccaccga tgctgctcag agaaagtttg
     1561 gtgccgccta aaaactcggt tgctcgtatc gttttgttgc ttgctaaacg gtaaatggcg
     1621 cgaagattgg ctcccagggc aagtttacca ttcattagta agtgggacac tccgaagttc
     1681 ggaccggcat ctagataggc ctgggaatca acatcggcat ctgcgcgaag catgatgtta
     1741 gcttgaagat tcgttaataa accaaacgaa aacccagtat tttcaccgaa gagcttcggt
     1801 gagtagtagc cgaaaaggcc agtaaaacga aagtgcatgt tttcaccagc aattccttca
     1861 tcggcgattt ttgaaatggc cgaggctccg gaactacctt tgatgtcatt gacggcacta
     1921 aaattcgaaa tcaaattagt attggcttcg aatgttgggc tcaagatttc aatgcgcgac
     1981 tcaggatgtt cgcttgccat agctggattc gcgaatagag cttcgtcgta catgccggtt
     2041 gtaaatgcca cgccacccat accgagactt ctaaggccgc ggtaggtttt catgatttcg
     2101 tttagacttc caaaggctgg agtggttgcg agggccgcaa ccgcacaagc taagattgtt
     2161 attttctgac tatgtttcat tttgttcacc taattgattg tagagttcct gttatatgtt
     2221 aagcccggag ccttggctta ttcggctgga agcatctggg atataatgta acatcggttg
     2281 attgcgcccg tagaggcaga ggcaatccca ccgagttggc tagctaaggt acccaaattt
     2341 ccaagagtag aattgcttcc gatttcatta ttagcggcaa tgagtgcgtt tcctactacg
     2401 tttgcatcag tgttcgacat tcgcgctggg caggtaatat cggtagcggc cgagcagctc
     2461 acttgttggc agctttcaat tgcaatatcg gttttaaaca cccgttggcc gttggtgctg
     2521 tactttgcaa tataaccagc cgcctttacc atttgcgcaa tggcgataac caaagatcta
     2581 tcggtggatg ttcccatctg accaaaagca ttgattgcct catctatata cgaaagtttg
     2641 gtgtcgttgg ttggaaccat ttcgtttgca agtctaccaa caaccgtcag gttgttaaga
     2701 gtcgtgtcaa atgaatagag gctcttgccg acgcgcccga tcgtagcacc agcgcgacag
     2761 agattagccc agccgcgtag gcgcgcgatt tccgcatcag gttgattgat atcgtttagc
     2821 ttttgaatcg cgtcttcaca cttggcttcg tctagaagtg ctttgacttc agaaagtttt
     2881 tctgtcgcgg tttctggtgt gtcgattccg cctgtggcat tacagctcat cattgaaaaa
     2941 gaagaaattg ttacaaaaag tactgctaat attgagaacg ttctgttgct cattttgaac
     3001 ccccgtgcaa agattctcag taatattctt agtttggaca agtgaaattt gaagaaacct
     3061 aaaaaaaaaa gtaataaact tgacgtagtt tttcttcacc ccgagtcctt tggtcgtact
     3121 aaggcagctc gcgctttaaa aaagtcgtta gtacaaatgt tgaatgcctg ccgagaccta
     3181 aaattgataa gctctgggac cactgtggtt tcgatcagtc tggtcgatga tcgagaaatg
     3241 aaaaaattga atcgtgcata tcgaaaaaaa aacgcgctta ctgacgtttt aagtttcgaa
     3301 caaccgatcg gggatttgtc cgaaaatcct aaaggagtaa ggtttttggg ggatatcgtg
     3361 ttttgccgat ctgtggttcg aacccaggcc cgaacctacg ggcactcggt agagattgag
     3421 ttaaaaatat tgctagccca tgggcttttg cacctcttgg gaatggatca tgagcgatcc
     3481 agtcgcgcct taaaaaaaat gctcggattt gagcgccaag ttcttttagc cgctggagtg
     3541 cgtcaatccc ctggactcct cgaccgggcc aagttatgaa cgagatctat gagtcaaatt
     3601 ggtcaaagtt cagagatccg tgagggtctg aaagctatcg gcgaaaaact cggtttccta
     3661 cgaggttcgc tttgacatcg ctaacaaaga gcgtcgatta aacgaaatcg cggacctttc
     3721 tacccaggca gagttttggg ctgataatcg caaggcccag tccattctta aagaaaaagg
     3781 ccggctcgaa aacgaacttg gcacctttaa aaaaagctat cgtgactatg aagacttaaa
     3841 tgcgttgttc gagttagcag aagagtcggg cgacgaatca ttgtacgccg acgctcgagc
     3901 acagttgacg ctactttttg ggtcggtcga gaatatagag ctccagaaaa tgctctccgg
     3961 cgagcaagat ggtttgaacg ccattgttag tattaatgcg ggtgccgggg gaaccgaatc
     4021 ccaggactgg gctcagatgc tattgcgcat gtatcttcgt tgggcccaac aaagtggcta
     4081 caagaccgag gtggtagata tcattgaagg cgaacaggcg ggcattaaga gtgcaaccgt
     4141 gattatcagc ggggtgaacg cgttcggatt tttaaagtca gagattggaa ttcaccgcct
     4201 tgtaagaatt tcgccatttg attcaaacgc aagacgccac ccctcttttg cctcggtctt
     4261 cgtgacgccc gaggtcgacg ataattttga agttgaaatt cgcgacgtcg atttacgtgt
     4321 cgacgtgtat cgcgccggcg gcaagggtgg ccaaggcgtg aacactacag actcggcggt
     4381 gcgcctgacg cactttccga ctggcatcgt agtgcagtgt cagaacgagc gatcgcaaat
     4441 caaaaataaa gcgatggcga tgaaaatttt aaaatcgcgc atttatgaac gccacatgga
     4501 agaagaacga aaaaaggctg ccgaagtcga agctggaaaa aaagatatcg cttggggcag
     4561 tcaaattcgg tcctatgtgt tgcatcccta caaaatggtc aaagaccatc gaactgggtt
     4621 tgtttcgtcg caggcggaga aggttttaga cggagatctc gacggtcata ttaaatctta
     4681 cttgcgctgt tcgctcacgg gtgagtgggc gcgtggtggc gactccgaca ttgactaaat
     4741 ccaaaaaccc cttaacctga taagaggggg caaattacgc gtgtcgattg gccgagaaat
     4801 atatgttgga actgtaggtt acaattatcg cgactggatc ggatcgtttt accccaaagg
     4861 cgcgtcgtcg cgccgccagc tacagctcta cagtaagcag ttcaatgtgt gtgaacttac
     4921 ccagtttacc taccaaatgc ccgaaatcga ccgcatgcaa aacttcgtgg aagggttggg
     4981 ttcaagtcga accaagtttt ttgtacgcat tcacaatgca ctcactcacg gcaccgatat
     5041 cggattagcg ctcactgttg cgcgccattt tcgccgtgct cttgaacccc tggtcgagac
     5101 tggtcgctta gccgggtttg tggcgacttt tccttatgcg tttaagaata gcgacgcgtc
     5161 acgaatgtat ttggaacaat tggccatttc ccttgcgtgt ccggatctgc cgctgtttgt
     5221 ggattttcgc cattcgactt gggtaacgcc gaagtctttt cagtggatgg ccaaacggaa
     5281 cctgtcgttt gtgtgtgtag acgaaccgga gcttccgggg cttatgccgc cgctagtaac
     5341 ggcgacccaa gattcggtct tgataagatt gcacgggcgc aatgcgacgg ggtggtggtc
     5401 gggtaatctg gtcacccgtt acgactattt gtattcgaaa gacgaactta gcggactatt
     5461 aactcggtac ctgccgcttt tagagcaggg taaacaggct cattttttgt tcgcaaatca
     5521 ctggcaggct cagtccattc gtaatgggct ggagtggcgg tcgatgctcg cggaatatga
     5581 aatgggggag agtcccactc gcgcaggcat tgccgacgaa gacgtgttga gggcccccgt
     5641 ggcgttgccg ttagaggacg tgaagagccg cgtcagtctg gccttgtcag atcttcagat
     5701 atccatggac ctacccccca gcgttgaccc agaatcgggt tgacaggatc gcccggcaag
     5761 actaaaacca gacacctaat tgtttgctgc ggccaacagt cctagaagcc ctttagcggg
     5821 ggtgtagttg agttggttac aacgccgccc tgtcacggcg gaggccgcgg gttcgagtcc
     5881 cgtcactccc gccattttac ttctaaccac ttgatttcac tgacgaaaaa atagacaact
     5941 cctcctgtgg gggacattgg gggacaggtg ccctgtttcc agcaatgtag accacttttg
     6001 aagacataga atttgct
//
LOCUS       DAOMXO010000045         5947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7068_length_5947_cov_5.103021, whole genome shotgun sequence.
ACCESSION   DAOMXO010000045 DAOMXO010000000
VERSION     DAOMXO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7068_length_5947_cov_5.103021"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..3011
                     /locus_tag="PLH57_01745"
     CDS             189..3011
                     /locus_tag="PLH57_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01362.1"
                     /translation="MHTNVRTIGGSCIIPACGTGQIVDFTTCSCRSATTGGGGTTQPG
                     YEDQLTPFLRVGFYVGTYYCNSSSGSDQNLCLDELVPAAPPPEPQLATGPNVHKYSTL
                     SPFNVHQDVGYLRWDLAIDASQYGAGEALSKGTPYFGTFATLIGNHSFEYRLKPNTYK
                     SSNNIQWQDCVEHFSGSSTSQVASFNRCDLRTGSVSTTAPAAASIPLTATTNSSGLAL
                     IERLSMSGTSANRNLIDEDAGSTTSTEYGNPIALHGFGLSKYNVDDGNAATFDTAGPG
                     LFSNLGYYKYSWGPFAVENSSGNSLSSTGTITSSTSAVTGLHFLPVSVVESSALAPDS
                     EDGTVESRTCSHASDPCAVTAVHRTTGGNIAIQNFSPKYGNNHSSSSSTLPGGITAVG
                     TPQIIEDTSQLSTSTTSSTTPARLRVFARATDGNIYMNYTNNRRWQSWTSLGRPWMCS
                     AEAGAAPLDCSDPANDTENACCAKLINTAPYRTFSAFVSDYTAVPELSPNDGISIAGE
                     PVVLSYMDTVSGIATDVVQGYIMILVRVSHLQSSDGALGTYHNAVFYNYARSTNGEVS
                     SGSTLDFDNLSNWSGWMPVKDNDGNIFRIQGDPVGTILEVVAGTVRAHIFGTLLDSDN
                     GADPGSGTAVLHPPIGPSSGETTAAAPATQRWYNYGNVIIRTAYTISAAVPATGTAND
                     FVNAPNWDDIESDSRTNGTSAIIGNLSLHTRNSSIIQIYAEHLLTATHSDVNNTSPQS
                     FLKRVAVYRYSAAGAEDPSGGGVDRVQIFDNITFVGAPQPAYVGPNTNDHDEALDGVA
                     IAANTILGTDRVFLVGRAICPSTDGGTSYPCDRAIPSVAYMRFDETLTMNDSDAVAYF
                     NGVAYDRDSNTYNTSAIRVSPIVATSDVVPIYSNSLDNDGADVPVYIFVRDGNGRLAV
                     GRFLRYNAGTSGAAIWNFVVTGGYVN"
     gene            complement(2989..3483)
                     /locus_tag="PLH57_01750"
     CDS             complement(2989..3483)
                     /locus_tag="PLH57_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRK01363.1"
                     /translation="MDSALVKIFGLSQFLILGLIVGTVVVVFVKQWLRDKALEKLHDE
                     QNNTQTDALRKNSAVKRVGVPESGQSRSDNTEHHQPREKGRPQLASAFPSWTDATPAH
                     EILEVAIDADAGAIESAYKRQLKKYHPDRFGGWGVEYAKRAHQIILLLQKSRDRMLNS
                     RNHR"
     gene            3585..4562
                     /locus_tag="PLH57_01755"
     CDS             3585..4562
                     /locus_tag="PLH57_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRK01364.1"
                     /translation="MFLFVLAALHVSMTYWSQPPARPKSEFGNLTFNARKTSPAPWIP
                     EFFKNWRRHFIQGTQKSDSTGLVAGIFLGATDRISNEHYALFQQAGLLHTLAASGSNC
                     WIVAMLLHGFFAFLLQPLSLRFFPSRLTLRIKKDLLHWSFLAGGLIFFLWSDQSPPIA
                     RALILIVTRFLFFVFDHRAPFWRILLVHYSLSLIIAPTLWFNVSFQLTYSCIAGMVAA
                     HKIKAGLFPNQHGSLLDYVVTTLGASIGATPITFIVFGEINFTGLFTNAIIGPLISLT
                     IMPLALIGMLLAHTPLVGVPIWLAAQFCKFGVVLLSTTLDFVPALQYFH"
     gene            4565..5182
                     /locus_tag="PLH57_01760"
     CDS             4565..5182
                     /locus_tag="PLH57_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRK01365.1"
                     /translation="MAKNLEPEIVFENNDFLVINKPAGLDSQNSKESRFSVVEWMRNR
                     YGYAGLVHRLDLNTSGLMACAKNEKAAKKLTDSLIAGKMRRSYIAVVIGKLPNDQGQI
                     THELDDKTSLTHYKVAERFANSTLVEVDLETGRKHQIRRHFALEGHPLLGDHLYKKAG
                     SHLLFNRPALHAHSLVIDGQRYQALLPADIVKLVDRLRAVKSKTP"
     gene            complement(5136..>5947)
                     /locus_tag="PLH57_01765"
     CDS             complement(5136..>5947)
                     /locus_tag="PLH57_01765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK01366.1"
                     /translation="NGQWFLFVISRKGLTKSELIKIAENFPHGEVAIVAGAAKANTSA
                     KSEPVEPVAFAEKEELVAIEGETHYALEAEAVLNPGLFLDQSKNRNFLKMNCLKGQTL
                     LNLFCYTSSFSVVAAKQGVVTTSVDVSARYLKWSQKNFALNELQTGGHRFLKADARDF
                     VRREAAKKRKFDWVIVDPPTFSRADGKVFQIEKHLEPLLCDVLKSWTEKGGVLVSTND
                     ASWDEADFEGCLRRATNENHRLRRIEIPTEFGTHYPLKGFWILPRASGRQA"
BASE COUNT         1429 a         1600 c         1367 g         1551 t
ORIGIN      
        1 agtgcctcgg cgtcgccgct gcgaatcagc gtcacggcca tcgccgccgc gtcgccgcgt
       61 caagctccag cagttcgtcg agtggcgtgt gttcaatttt tactgataat tctagagact
      121 actccacgtg cctttcgtgc gtaagttcat atggagcgag cgcttctgat aatacgatca
      181 tcgagtgcgt gcacactaac gttcgcacaa tcggaggttc ttgcattatt cccgcttgcg
      241 gaactgggca aattgtcgac ttcacgactt gctcgtgccg aagcgcaact actgggggcg
      301 gtggaacgac ccaaccaggt tacgaagatc agctgactcc gttcttgcga gttggctttt
      361 atgttggaac ttactactgc aattcttcga gcgggtccga tcaaaacctt tgcctagacg
      421 aactagttcc cgcagcgcct cctcctgaac cccagctggc cacgggcccc aatgtacaca
      481 aatatagtac gctttcgccg ttcaacgtgc atcaggatgt aggctatctt cgctgggatc
      541 tcgcgatcga tgcttctcaa tatggtgccg gtgaagcgct ttcaaaggga acgccatact
      601 ttggtacatt cgctacgctc attggcaatc actcgtttga gtatcgtcta aagcccaata
      661 cgtataaatc ttcaaacaat attcaatggc aagattgcgt agaacatttt agtgggtctt
      721 cgacatcaca agttgcgagt tttaaccgct gtgatcttcg aactgggagc gtttcaacca
      781 cggctcccgc tgccgcatcg attccactga ctgctacgac aaactcgtcg gggcttgccc
      841 tgattgaaag actttcaatg agcggcacat cggccaatcg taatttgata gacgaagacg
      901 ccggatcaac aaccagcaca gagtacggga atccgatcgc gctgcacgga tttggacttt
      961 caaaatataa tgtagacgat ggcaatgctg caacgttcga tacggctggc cccggcctct
     1021 ttagtaatct aggttattac aaatactctt ggggcccttt cgctgtcgaa aactcgagtg
     1081 gaaactctct ttcgtctacc ggaacaatta ccagttcgac ttcagcggtc acgggcctac
     1141 attttctacc cgtaagtgtc gttgagagca gcgccctcgc gcccgactct gaggatggaa
     1201 cggtagaatc acgcacctgc tcacacgcaa gcgatccctg cgcagtcacc gcagttcacc
     1261 gaaccacggg aggaaacatt gcaatacaaa atttttctcc caaatacggc aataatcact
     1321 cttcgtcatc gagcacgttg cccggcggaa ttactgcggt tggaactccg caaattattg
     1381 aagatacttc gcagctaagt accagcacca cttcaagcac aactcctgcg cggcttcggg
     1441 tatttgcacg cgcgaccgac ggcaacatat acatgaacta caccaacaac cgccgctggc
     1501 aaagctggac aagccttgga agaccctgga tgtgcagcgc cgaagcgggc gcagcgccat
     1561 tagactgcag cgacccggcc aatgacaccg aaaacgcttg ctgtgcaaaa ttgatcaaca
     1621 ctgctcctta ccgcaccttt tcagcctttg tgtctgatta cacagcggtg cccgaactca
     1681 gcccaaacga cggcatcagt atcgcagggg aaccggtagt gctgtcttac atggatactg
     1741 tatcgggaat cgcgaccgat gtagtgcaag ggtacatcat gatccttgtg cgtgtttctc
     1801 atttacagtc gtcagacgga gcgctgggaa cgtaccataa tgctgtgttt tataattacg
     1861 ctcgatctac caacggggaa gtaagctcgg gtagcacgct tgatttcgac aatctcagca
     1921 attggtctgg ctggatgccc gtaaaagata atgatggcaa tatctttaga attcagggcg
     1981 atccagtcgg tactattcta gaagtagtag ccgggaccgt gcgggcgcat atatttggaa
     2041 ccctgctgga cagcgataat ggagctgacc ccggaagtgg aacagctgtg ctgcatccac
     2101 cgatcggtcc aagttcaggc gagaccaccg cggcggcacc agcaacccaa cgctggtata
     2161 actacggaaa cgttatcatt cgtactgcgt acacaattag cgccgcagta cccgcgactg
     2221 gcaccgcaaa tgatttcgta aatgccccta attgggatga tattgaatca gactctcgaa
     2281 ctaatggtac ttccgcaatt attggcaacc tgagtctaca cactcgcaac tcgagcatca
     2341 ttcaaattta tgcggagcat cttttaaccg ctactcactc ggacgttaac aatacgtcac
     2401 ctcagtcttt tttgaaaaga gtcgccgttt accgctatag tgcggccgga gctgaggacc
     2461 cttccggagg cggggtcgac agagtgcaaa tttttgacaa cattacattc gttggcgcgc
     2521 cgcaacccgc ctatgtgggc cccaatacaa acgaccatga cgaagcccta gacggtgtcg
     2581 ccattgcggc gaatacaatc ctaggtactg accgggtttt cttggtagga cgggccattt
     2641 gtccgtcgac tgacggaggc acttcatacc cctgcgaccg agcgatacca tctgtcgcct
     2701 acatgagatt tgacgaaact ctaactatga acgactctga tgccgtcgca tactttaatg
     2761 gcgtcgccta cgaccgagac tcaaatacct ataatacttc cgccatacgt gtatcaccta
     2821 ttgtggcaac cagtgacgtg gtacctattt atagtaatag cctcgacaac gatggcgctg
     2881 acgttccagt ttacatattt gtacgcgatg gaaacggtcg cttagccgtt gggagatttc
     2941 ttcgttacaa tgcgggaacc tccggagccg cgatctggaa ctttgtagtt accggtggtt
     3001 acgtgaatta agcattcgat cgcgcgattt ttggagtagc agaataatct ggtgagcacg
     3061 tttggcgtat tccacacccc agccaccaaa tctatccgga tgatactttt tcaactgcct
     3121 tttgtacgcg ctttcgatcg ctcccgcatc ggcgtcgata gccacttcaa gtatttcgtg
     3181 ggcgggcgta gcatcggtcc agctaggaaa ggcgctcgcc aactgagggc gacctttttc
     3241 gcgaggctgg tgatgctcgg tattgtcaga ccgactctgc ccggactcgg gtacccctac
     3301 ccgttttaca gcactgtttt ttcttaacgc atcggtctgg gtattgtttt gttcatcgtg
     3361 cagtttttcc aaggccttgt cgcgcagcca ttgttttacg aacaccacga cgaccgtgcc
     3421 aacaatgagc cctagaatta aaaactgact cagcccaaaa atttttacga gcgcactgtc
     3481 cattggctcg attttagcat ttcgttttta agatgtcgca agccagcaac aattctttta
     3541 gccgggcgcg tattgcgtgg ctggcacgtt tctaccgttg gcctttgttt ttgttcgttt
     3601 tagctgctct gcacgtaagc atgacctatt ggtcgcaacc cccagctcgt ccgaagtcgg
     3661 agttcggaaa ccttacgttc aacgcccgga aaacctcccc tgcgccatgg attccagaat
     3721 ttttcaaaaa ctggcgccgc cattttatcc agggcaccca aaaaagcgac tccacgggcc
     3781 tagtcgctgg aatatttcta ggcgctacgg accgcatttc aaacgaacac tatgcactat
     3841 ttcaacaagc gggccttcta cacacccttg ctgctagtgg ttccaactgt tggattgtgg
     3901 ccatgttgct tcatggtttt ttcgcatttc tgctgcaacc cttgagtcta cgtttttttc
     3961 cttcgcgcct cacgttgcgc ataaagaaag acctcttgca ttggagcttt ctcgcaggtg
     4021 ggctaatatt ttttctttgg tctgatcaaa gcccacctat tgcccgggcc ttaattctta
     4081 tagttacacg atttctgttt tttgttttcg accatcgggc gccgttttgg cgcatattac
     4141 tggtgcacta ttcgctctca ctaatcattg ctccaaccct ttggtttaac gtaagctttc
     4201 aactaaccta ctcttgtatt gctggaatgg tcgctgccca caaaataaaa gccggcttgt
     4261 tccctaacca gcacgggagc ttgctcgatt acgtggtcac cacgctagga gctagtattg
     4321 gtgccacacc gattaccttt attgttttcg gcgaaataaa ctttaccggt ctttttacca
     4381 acgcgatcat tggaccgctg atttcgctga caatcatgcc gctagccctg atcggcatgc
     4441 tactggcaca cacacccttg gttggggtac caatctggct cgcagctcag ttctgtaaat
     4501 ttggcgtagt actgctctcg accacactgg acttcgttcc cgcactccag tattttcatt
     4561 aagcttggca aaaaatttag aacccgaaat tgttttcgaa aataatgact ttctagtcat
     4621 taacaaaccc gctggacttg attcacagaa ctcaaaagaa tctcgtttca gtgtggtcga
     4681 atggatgcgc aaccgctacg gctatgcggg cctagtgcac cgattggacc taaacacttc
     4741 gggcttgatg gcgtgtgcaa aaaacgaaaa agcagcaaaa aaactgaccg actcgctaat
     4801 agcgggcaaa atgcgtcgtt cgtatattgc agtggtgatc ggcaaactgc ccaacgatca
     4861 aggtcagatc acccatgagc tcgacgataa gacttcgctc actcattaca aagttgccga
     4921 acgttttgcc aattcaacac ttgtagaggt cgacctcgaa actgggcgta aacaccaaat
     4981 cagacgccat tttgcgctcg aaggtcaccc gctccttggt gatcacctat acaaaaaagc
     5041 cggttctcat ttattattta atcgtccagc gcttcatgct cactcgcttg tgattgatgg
     5101 gcagcgctac caagccttat tgccggccga tattgttaag cttgtcgacc gcttgcgcgc
     5161 ggtaaaatcc aaaacccctt gagcggataa tgcgttccga attccgttgg aatttcgatt
     5221 cgccgcagtc gatgattttc gttggttgcg cggcgcaagc agccttcgaa gtccgcttcg
     5281 tcccaggaag cgtcattggt agacaccagt accccccctt tttcagtcca acttttcaaa
     5341 acgtcgcaca acagaggttc aagatgtttt tcgatctgaa acaccttacc atcggcgcgt
     5401 gaaaacgtcg ggggatccac aatcacccaa tcaaattttc gctttttcgc agcttcgcgg
     5461 cgcacaaagt ctcgtgcgtc ggcctttaga aatcgatgtc cgcctgtttg cagctcgttg
     5521 agcgcaaaat ttttctgcga ccattttaaa tagcgtgcac tcacatctac gctagtggtg
     5581 acaacaccct gcttcgctgc aacaactgaa aatgaagacg tataacaaaa taaatttaaa
     5641 agcgtctgac ctttgagaca gttcattttt aaaaaatttc tatttttcga ctgatccaaa
     5701 aaaagacctg ggtttaaaac tgcttcagcc tctagcgcat agtgggtttc accttcaatt
     5761 gctaccagtt cctctttttc tgcaaacgct accggttcaa ccggctcgga tttcgccgaa
     5821 gtattcgcct tggctgcccc ggcgacgatc gctacttcgc cgtggggaaa attctctgcg
     5881 atcttaatta attcactctt tgtaagaccc tttcgcgaaa tcacaaaaag aaaccactgc
     5941 ccgtttt
//
LOCUS       DAOMXO010000046         5884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7171_length_5884_cov_4.892606, whole genome shotgun sequence.
ACCESSION   DAOMXO010000046 DAOMXO010000000
VERSION     DAOMXO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7171_length_5884_cov_4.892606"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLH57_01770"
     CDS             <1..437
                     /locus_tag="PLH57_01770"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HRK01367.1"
                     /translation="ALAARHCADVCAGVNGAGFRGAEQMWYWLEVPLWLALGVFAQLL
                     IRFFSCVWVFWTERGWAISHLYYQFYQFATRPDSLYPWFLRYGIMTILPFAFIGSVPA
                     RALLSGLSLQEYGLLVGALGGFLWINIFLWRRGLRRYQSASS"
     gene            466..3186
                     /gene="polA"
                     /locus_tag="PLH57_01775"
     CDS             466..3186
                     /gene="polA"
                     /locus_tag="PLH57_01775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRK01368.1"
                     /translation="MSSKRETLFLVDASSFIFRAYYAIRRLTTSQGLPTNATFGFVTM
                     LLKVLEKYKPEYVTCVFDTPAPSFRKEMYSEYKANRGAPPDDLLPQFEHIHKAVEVLG
                     IHKIAEPGYEADDIIGTFARKFRDCDVVIVSTDKDLMQLVDERVSMLDTMKDIKYTPK
                     EVEEKLGVRPEQVQDYLGLIGDSSDNIPGVAGIGPKGAVELLREYGSLEGVLQGATSM
                     KAGKKRDALLEHEAIARLSKELATVKCDLDLVAANSIGAVSLSKLRAPAKVGAEFVQW
                     AKSLEFNALVAKYGGLLSEQEKNSSGESFSGVVSVGTAVPKTQIVGNRADWQKLIKSC
                     EAQPTIAFDTETRGDSVTELEIVGLSLAYSKESAFYVPLRHADHASDLDVATVLSEFQ
                     QLVRGKRVVAQNLKYDYKVLLAEGAGDLSENTTEMFDTMLAHYVLEPEQKHGLDALAS
                     RYLATTIGDFKETLGDRENFSLVPVKDAAQYAGLDAWSCFCLEDILRQDLTNRGLLEL
                     FKTIEMPVAPVLAHMEWNGVAVDKDILKTLSKEYGSELAKLETQIFELVGSEFNLNSP
                     KQLSEILFTKLKLPVIQKTKTGFSTDVTVLEKLAPLHPVPNLLLQYREYTKLLNTYIE
                     VLPNLIENDGRVHAHFNQAVTATGRLSSSDPNLQNIPIKTDRGRRIRQAFVAREGCVF
                     VGADYSQVELRLVAHLSGDEALIKAFRAGADVHRATAAEIFGVKPEQVSDEQRAAAKA
                     INFGLIYGKTPFGLSQELGISRANAAQYIAAYFERYSGVKEFMDQSLERARKHGEAVT
                     LFGRKRRLPDIQNKNGAVRANAERMAMNTPVQGTAADLIKIAMAKVHDRIRKTAARLV
                     LQVHDELVLEVPESQASEVESIVRDAMENSVKLDVPLIANIGTGKNWYNL"
     gene            complement(3202..3879)
                     /locus_tag="PLH57_01780"
     CDS             complement(3202..3879)
                     /locus_tag="PLH57_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01369.1"
                     /translation="MLTTDNLGPYCHAWAENFQKEHQASLSQIETLSPIAWYQGLYQF
                     FHDLKNSLLMSPENELKELLPAVEVCNALFSSLAEGKLDLAISDKKILRSVLERPDFS
                     LEDIKTTVEEVASNAVALKNLAIPFAKNFQWDSFATARAQSALRYGYRIFERTDVVSL
                     SKLENHDKMIDLYLKNLGGFSIVRKMEPKTIGGTVKNKIDDMRLECIQLAAVPGAAQP
                     SAPWRAI"
     gene            complement(3873..4235)
                     /locus_tag="PLH57_01785"
     CDS             complement(3873..4235)
                     /locus_tag="PLH57_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01370.1"
                     /translation="MNTTLRDWLALSLLDDAEHTLRRQVSPQLLDELVRLSVKSKSNA
                     ARPVPKPALPSLSQGSPNAHNPLFGSKPTLRELMNQYNGRSTAKKLLKNPDQQNFSFD
                     RIESIRLPSIPGKILRPC"
     gene            complement(4232..4672)
                     /locus_tag="PLH57_01790"
     CDS             complement(4232..4672)
                     /locus_tag="PLH57_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HRK01371.1"
                     /translation="MQDLGDNQTRIRLEDLLVSESASTTPTLLVCGIQEHERPLKGAA
                     GEIDWRLKGFLSRFLIRGHITGKTGEVTYVPVIVRGTTRHILLVGTHENASARENQTL
                     FGKINATMKNLNLSKVALSVRSFPKTSPSEIAQHFSNVQVEIAQ"
     gene            complement(4679..5110)
                     /gene="nusB"
                     /locus_tag="PLH57_01795"
     CDS             complement(4679..5110)
                     /gene="nusB"
                     /locus_tag="PLH57_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRK01372.1"
                     /translation="MSNSRRASRETAFQFLYGLLPLPAPGGKTSDCPFSRAEFSLFCS
                     SFEKVEDDFAWELVEGTGKNLPLIDQEIHANSTNWRIERMPLVDLTVLRMGAFEILFH
                     PDIPKTVAINEAVDLAKKYGAEDSHAFVNGLLDKLEKKSVK"
     gene            complement(5107..5574)
                     /gene="nrdR"
                     /locus_tag="PLH57_01800"
     CDS             complement(5107..5574)
                     /gene="nrdR"
                     /locus_tag="PLH57_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRK01373.1"
                     /translation="MRCPYCSTVENKVIESRINQTGDITRRRRECLHCQGRFTTYEKV
                     ENVMPSIVKKDGRREPFSRDKIFSGIQKACQKRPITTEQIEKIVTQIESKIASFNVHE
                     LPARTLGHLVMAALHDLDKVAYVRFASVYREFKDVEEFVAELKQESPQQEGTL"
     gene            complement(5583..>5884)
                     /gene="glyA"
                     /locus_tag="PLH57_01805"
     CDS             complement(5583..>5884)
                     /gene="glyA"
                     /locus_tag="PLH57_01805"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006917962.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRK01374.1"
                     /translation="LIDLTNKDLTGKDAEAALGRAHITVNKNTVPFETRSPFITSGVR
                     IGVPAVTTRGMKQQHIRQIVGWIDAALKASQNEERLHRIGEEVKDFCKSFPLFAQ"
BASE COUNT         1465 a         1297 c         1583 g         1539 t
ORIGIN      
        1 ttgcgctggc tgcccgacac tgcgccgacg tctgtgcagg tgtcaatgga gccggatttc
       61 gcggagcaga gcaaatgtgg tactggctgg aagtgccact ttggctggcc ttaggtgtgt
      121 ttgcgcaact gttgattcgg ttcttctcat gtgtatgggt gttttggacg gaacgagggt
      181 gggcgatttc tcatctgtat tatcaattct atcaattcgc cacgcggcca gactcgttgt
      241 atccctggtt tttgcgttat gggatcatga ctattctgcc gttcgcattc attggaagtg
      301 tgcctgcacg cgcgttgttg tcaggtcttt cactacaaga gtacggcctt ttggtcggag
      361 ctcttggtgg atttttgtgg atcaatatat ttctgtggcg acgcggactg cgccgctatc
      421 aaagtgccag cagttagtgc gggaccggtg aaaggaaatc aagtgatgtc gtccaagcga
      481 gagactctgt ttttagtcga cgcgagctct tttatttttc gggcttatta tgcgatacga
      541 cggctcacga ccagtcaggg tttaccgacc aacgctacat tcgggttcgt aacgatgctc
      601 ttgaaggttt tggagaagta taaaccagag tacgtgacct gtgtgtttga tacgccggct
      661 ccgagttttc gaaaggaaat gtattccgag tacaaagcca atcgaggagc accaccggac
      721 gacctgctac cgcagttcga acacattcat aaagcagttg aagtactggg aattcacaaa
      781 attgcggaac caggttatga ggccgacgat attatcggaa cgtttgcacg taagttccgc
      841 gattgcgacg tagtgattgt gagcaccgat aaggatctca tgcagttggt cgacgagcgc
      901 gtgtcgatgc tcgatacgat gaaggacata aagtacacgc ctaaggaagt cgaagaaaag
      961 ttaggggtcc gacctgaaca ggtgcaagac tatttgggtc tgatcggcga cagctcggac
     1021 aacattcccg gagtagcggg aattggtcca aagggcgctg ttgagctttt acgggaatac
     1081 ggctcattag aaggagtact tcaaggtgcg acctcgatga aggccggaaa aaaacgagac
     1141 gcgcttttag agcacgaggc gattgcgcgc ctatcgaagg aactggccac cgtaaagtgt
     1201 gatcttgatc tggtggctgc caatagcatc ggagcggttt cgctctctaa actgcgagct
     1261 cctgcgaaag ttggagctga gtttgttcaa tgggccaaaa gtcttgaatt caacgcgcta
     1321 gtggcgaagt acggcgggct acttagtgaa caagaaaaaa actcttctgg agaatcattt
     1381 tctggtgtgg tctcggtagg gacagcggtt ccgaaaacgc aaattgttgg gaatcgggcg
     1441 gattggcaaa aattaataaa atcctgtgaa gctcaaccaa ctatagcgtt tgataccgaa
     1501 acgcgaggag atagtgttac agaacttgaa atcgtaggat taagtttggc gtattctaaa
     1561 gagagtgcct tctatgttcc gctgaggcac gctgaccatg cgtcagacct cgatgttgca
     1621 acagtgttgt cggagtttca gcagttagtg cgagggaagc gggtcgtcgc acaaaatctg
     1681 aagtatgact ataaagttct attggcagag ggtgccggag acctatctga aaataccacg
     1741 gaaatgttcg acacgatgct cgctcattac gtactcgagc cagagcaaaa acacggtctg
     1801 gatgcgcttg cgtctcgtta cctggctacg acaatcggag attttaagga aacgctgggt
     1861 gatcgcgaaa atttttcatt agttcccgta aaagacgccg cgcaatacgc ggggctcgac
     1921 gcttggtcct gtttttgttt agaagatatt ctaagacagg atctgaccaa tcgtgggctc
     1981 ttggaactgt ttaaaaccat tgaaatgccg gttgccccag tgcttgccca tatggagtgg
     2041 aatggggtgg cggttgacaa ggatatactc aaaacacttt ccaaagaata tggtagcgaa
     2101 ttagctaaac ttgaaacgca aatttttgaa ctcgtcggga gcgagtttaa tttaaattcc
     2161 ccgaaacaac tctcagaaat acttttcaca aaacttaagc tgccggtaat tcagaaaacg
     2221 aaaacggggt tttcaacgga cgtaacggtt ctcgagaaac tggctccgct gcatccggtg
     2281 ccaaacctat tgttgcagta ccgagagtat acaaaactac tgaacaccta tatcgaagtc
     2341 ttgccgaact tgattgaaaa cgatggcaga gtacatgccc acttcaatca ggccgttaca
     2401 gctaccgggc gtctttccag ttctgaccct aatctccaga atatcccaat taagacggac
     2461 cgaggaagac gaattcgtca ggcgttcgtg gctcgtgaag ggtgtgtgtt cgttggcgcg
     2521 gactactcgc aggtcgaact gcgactggtt gctcatttaa gtggagacga agccttgata
     2581 aaggctttta gggcgggtgc cgatgtgcac cgagccaccg cagcggaaat atttggcgtg
     2641 aaacccgagc aggtgagcga cgagcaaaga gcagcagcga aggccataaa ttttggtttg
     2701 atttacggta aaactccgtt cggactcagt caggagttgg gaatttctcg ggcaaacgct
     2761 gcccaatata ttgcggccta ttttgagcgc tatagtggag tgaaagaatt catggatcaa
     2821 tcgctcgagc gcgcgcgaaa acacggagaa gcggtgacat tgttcgggcg aaagcgtcga
     2881 ctgcccgaca ttcagaacaa aaacggtgcg gtgcgcgcga atgccgagcg aatggcaatg
     2941 aatacgccgg ttcagggcac ggcggctgat ctcattaaaa tagccatggc caaggtgcac
     3001 gatagaatcc gaaaaactgc tgcacgactc gtattacagg ttcacgatga actggtgctg
     3061 gaagtgccgg aaagccaagc gagcgaagtc gaaagtatcg tgcgagatgc aatggaaaac
     3121 tccgtcaagc ttgacgtgcc tttgatcgcc aatattggga ccggaaaaaa ttggtacaat
     3181 ctataacggt gatggatgtt gctaaattgc tcgccaagga gcggagggtt gcgcagcccc
     3241 agggacagca gctagttgaa tgcactcaag tcgcatgtcg tcgatcttgt tctttaccgt
     3301 accgccaatg gttttaggct ccatttttcg tacaatgctg aagccaccca gattctttag
     3361 ataaagatca atcattttgt catggttctc tagtttcgaa agactcacta cgtctgtgcg
     3421 ttcaaatatc ctgtaaccat aacgaagcgc cgactgagct ctggccgtag cgaacgagtc
     3481 ccattggaaa ttttttgcaa aaggaatagc taaatttttc aaggctacgg cgttcgaggc
     3541 cacttcttcg accgtggttt taatgtcttc tagcgagaag tctgggcgtt ccagaaccga
     3601 acgcagtatt tttttatcag atattgcgag gtctagtttg ccttcagcta gagacgaaaa
     3661 tagtgcgttg cagacttcta ctgccggtaa aagttccttt agttcgtttt caggtgacat
     3721 gaggagcgaa ttctttaagt cgtgaaaaaa ctggtaaaga ccttgatacc aggcgatcgg
     3781 actaagcgtt tcgatttgag agagactggc ttgatgttcc ttttggaagt tttcggccca
     3841 ggcgtggcaa tagggtccaa gattgtcggt ggttaacatg gtcgaagaat ttttcctgga
     3901 atcgagggca ggcgaattga ttcgattcga tcgaacgaaa agttctgttg gtctggattc
     3961 tttagtaatt tctttgcagt cgagcgcccg ttgtactgat tcattagttc tcgtagcgtc
     4021 ggtttagaac caaacagcgg attatgggcg ttgggcgagc cctgtgaaag agacggcaat
     4081 gcgggctttg gtacaggcct cgcagcgttg gatttacttt ttactgacaa tcttaccagt
     4141 tcatccagaa gttgggggct gacctgtcgg cgcaacgtgt gttccgcatc gtctaacaaa
     4201 ctcaaagcaa gccagtcgcg aagcgtcgtg ttcattgtgc gatctccact tgaacgttcg
     4261 aaaaatgctg tgcgatttct gagggggaag tcttgggaaa actgcgaacg cttagagcca
     4321 ctttggagag atttaaattt ttcatggtgg cgtttatttt accaaatagc gtttggtttt
     4381 ctcgcgcact ggcgttttca tgggttccga ctaatagaat atgtctagtg gttcctctta
     4441 caataaccgg aacataggtt acttcgccgg tcttaccggt gatgtgacca cgaattaaaa
     4501 agcgcgacag aaaacctttt aggcgccaat cgatttcgcc ggctgcgcct ttaagggggc
     4561 gctcgtgttc ctgaatgcca cacaccagca gcgtgggagt ggtggaagcg ctctctgata
     4621 cgagaaggtc ttcgagtcga attcgagtct ggttgtcacc gaggtcctgc atcgagatct
     4681 attttacaga tttcttctcg agcttgtcca ggagtccgtt gacgaatgcg tgagaatctt
     4741 ctgccccgta ctttttggca agatcgacgg cctcgttgat agcgacagtt tttggaatgt
     4801 ccggatgaaa taatatctcg aaggcgccca tccgtagcac ggttaggtca acaagcggca
     4861 tacgctcaat acgccaatta gtggagttgg cgtggatctc ttgatcgata aggggaagat
     4921 ttttcccggt accctcgacc agctcccagg cgaaatcgtc ttcgactttt tcgaaactag
     4981 aacagaaaag tgaaaactcg gcccgactaa acggacagtc agatgttttg ccgccaggcg
     5041 caggaagtgg gagtagcccg tagagaaatt gaaatgcagt ttctcgagat gctcggcgtg
     5101 aattactcat aatgtgcctt cttgttgcgg cgattcttgt ttaagttccg caacgaactc
     5161 ttcaacgtct ttaaactcgc gataaacact ggcgaaccga acgtatgcta ccttgtcgag
     5221 atcgtgtagg gcggccatca cgaggtgccc tagcgtgcgt gctgggagct cgtgaacatt
     5281 gaaactggca atcttcgatt cgatttgtgt tacgattttt tcgatttgct cggtagtgat
     5341 aggtcttttt tggcaggcct tttgaatgcc tgaaaaaatt ttgtctcgtg aaaacggttc
     5401 acgccgcccg tcttttttaa cgatcgaggg cataacgttt tcaacttttt cataggtggt
     5461 aaatcgtccc tggcaatgca aacattcgcg tctgcgccgc gtaatatcac ccgtttggtt
     5521 gattcgggac tcgatcacct tgttctcgac cgtcgagcag tagggacaac gcatagacca
     5581 tcctattgtg caaagagcgg gaaggattta cagaaatcct ttacctcttc gccaattcgg
     5641 tggagccgct cttcgttttg tgaggccttc aaagcagcgt ctatccagcc gacgatttgg
     5701 cgaatgtgct gctgcttcat accacgcgtc gttactgcag gcacgccaat tcgcacgccg
     5761 ctagtgatga aaggtgaccg agtttcaaac ggaacggtgt ttttgttgac ggtaatatgg
     5821 gctcttccga gagcagcctc tgcgtccttg ccggttaagt ccttattagt caggtctatt
     5881 aaaa
//
LOCUS       DAOMXO010000047         5884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7172_length_5884_cov_4.755533, whole genome shotgun sequence.
ACCESSION   DAOMXO010000047 DAOMXO010000000
VERSION     DAOMXO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7172_length_5884_cov_4.755533"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /locus_tag="PLH57_01810"
     CDS             <1..1654
                     /locus_tag="PLH57_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRK01375.1"
                     /translation="TWGCAAVFLFLTLPLAFERPSGGRSAERQGFTAGIIRMYCHNPS
                     HSILKGPQIHFGNNPFNLEYTGFEWLQGVLARGLGGTECEGAVEKIGKSVSIWFSAAS
                     LLVLGALSFALAQLVGIATPFALIGSAVSVFLLACDELWLRYATYTMVENRMLFFALV
                     ALYGSVRTEAGSKAGMARYVWPLIAGSAWAMTGVHKPQGFAFFAVFWTVFLAIRWRIQ
                     GIAPSVRGILALGTGSLVVVGWLAWGHVLGQASDLPWILHTGPRATRWYFGNWSERFE
                     PKFFTSNLLKWAQQTGIFLVLPALAVLSIFFRRPVKAWALKFAQVAVPYGIALFVFVF
                     LFWAVYIVHEYYALPFDVGRALATGIALALLAEVVLGTIDKHHRLLALALAVLVTAVC
                     GTAYRKGVPPYLAYAARINDPRAPDYLHGWNEQVFPERHPFVVMTVRSRGSELLYLYK
                     AKARGFVWCTHNPIHAPRKYWKDQGVRWVAWGEPSPDPATPGLYQWTVRSIEQELELA
                     RKNNWSSDVDDHWAGRTMAQWAAHASSNGKDPCLDNPDPRSW"
     gene            1726..2817
                     /locus_tag="PLH57_01815"
     CDS             1726..2817
                     /locus_tag="PLH57_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01376.1"
                     /translation="MKHSQPIAKLLLLLLPVLPVFLGVDACHDPTIYNNIDDLSALFE
                     EESRLYGELDNVRVTSTQTNNQQWLPLAEAFVEWSDHLKVLSEKSEELVRNYNALSIE
                     RKHGYKARILALEPLYENFFRRPIRWTLPYEPWTYRITWENSFAIQRAEAMRPHFGVL
                     YNRANDTPSLNWSINMVQDSNPSGLATAVRNAWKARPALLICTGSSSLACGLKSQIAT
                     YFSSHLNRQLQIQNDDWIFSETFQPNLIFEKLTPNPADPYLVYGEPVAIRVADTQQYL
                     FASETAPGDFTVSTQTLVGGGVWPWQWELHPIWERNSNRTVQAVVGFGLYNRVTQMYL
                     VDCPRSAKTIAWYQGSLDSRNMTDLYQCQ"
     gene            complement(2819..3541)
                     /locus_tag="PLH57_01820"
     CDS             complement(2819..3541)
                     /locus_tag="PLH57_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01377.1"
                     /translation="MRVLLLAVSLAALGFYFFLRPNRPFEDSKQLAINKSVELTPGAQ
                     PNVNPVTESKPSVTTISHEEPMRSPEVEPISKTINDSNEVTDLNEVPQFLNRAALKES
                     KPQVDTYDVVRDDRFLRDFRGIFEGKATSTSDNVRFAPMYLEMNIEVVNERQQGRIYV
                     EMARPGQGLLLNLPIRISELRRKAGALWITVESAAGSPRSDTVYYQVYTLRELDAVAG
                     NVYRLRRGELRNTETFVLQRKN"
     gene            complement(3554..5296)
                     /locus_tag="PLH57_01825"
     CDS             complement(3554..5296)
                     /locus_tag="PLH57_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRK01378.1"
                     /translation="MSDNKQISFMRSLCMGEIEEELIIPFPQLNENELGTVKDILPTI
                     DNWLKDRGPDFRKWDRQAELPPEFIEEMKQMGLFGVIIPEEHGGLGLGATAYSRVLQQ
                     VARHDGSVAITAGAHSSIGMRGLLLFGTPEQKNKYMPKLASGEMIAAFCLTEPGAGSD
                     AASIKTKAVREGKEWVLNGEKLWITNGGIAQFFTVFARTEKEEGGQMTAFIVTRDMPG
                     VSVGPHEDKMGLRASSTTTVFFENVRLSDEHILGEPGKGFKVAMKVLNSGRTGLGGGC
                     VGAMKHVITEATKQAKERTQFGKPIAEYGLVKQKIGHMIVECYASEAAVNMVAGLVDQ
                     GYEDYAVEAAISKVFATECLWRTADEGLQIAGGNGYMCEFPYERIVRDCRVNRIFEGT
                     NDILRLFIALTAMNDVGKQLKEISKSLDGIFDDPIKGFGVLSDYARRRLSAATGVANE
                     KGTFTKIHPALKDYSTVFEEGVRDLSAAADRILRKHGKNIIGKQFATKRLADIMIDLF
                     VLACTLSRVNSSVAAKGIANCTKEIEILTVFSGQVRRRTKGNFGKIDNNDDELIKSLA
                     DHAYEIEGYGWDTI"
     gene            complement(5433..>5884)
                     /locus_tag="PLH57_01830"
     CDS             complement(5433..>5884)
                     /locus_tag="PLH57_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01379.1"
                     /translation="MAKARGFDGESSRVKRVVVMTLLETEKAPQGSEKREEQTYVLEL
                     FPAPPQERKKGRGRFDGDRKGGRGKGKGGRGGKRGERGGRGGDRSERRAQNKDGRPPR
                     QPRPQTPSKPLVIPKPRALPTDAPAASTSESAVSTAASSESSGSSAS"
BASE COUNT         1353 a         1563 c         1401 g         1567 t
ORIGIN      
        1 gacttggggt tgtgctgcgg tttttctttt tcttactctc cccttagctt ttgaacggcc
       61 ttctggcgga agaagcgctg agcgccaggg ttttaccgcc ggaatcatac gaatgtattg
      121 ccacaatccc tctcattcga ttttaaaggg accgcaaatt cacttcggaa acaatccatt
      181 caatctcgag tacacgggtt ttgaatggct gcagggagtg cttgctcgtg gtctgggcgg
      241 aaccgaatgc gaaggcgcgg ttgaaaaaat cggaaaatct gtgagcattt ggttttcggc
      301 agcaagccta ctggtgttgg gcgcattgtc ttttgcgctc gcccagctgg tagggattgc
      361 aactccattc gcattgattg ggagcgcggt ttcggtattt ttactcgcgt gtgatgagct
      421 ctggcttcgc tatgcaacgt atacaatggt cgaaaatcga atgttgttct tcgctctggt
      481 cgcactctat gggagtgtgc ggaccgaagc ggggtctaaa gctggaatgg ctcgctacgt
      541 gtggcctctg atagccggat cggcttgggc gatgaccgga gtacacaaac cgcagggctt
      601 cgcatttttc gctgtttttt ggacagtctt tcttgccatt cgctggcgaa ttcagggaat
      661 tgcaccaagt gttcgcggca tattggcgct gggaaccgga agtctggtcg tagtggggtg
      721 gcttgcctgg ggccacgtac tcggtcaagc ttcggaccta ccttggatat tgcatactgg
      781 gcccagagcg actcgttggt attttgggaa ctggagtgaa cgttttgagc caaagttttt
      841 cacttccaat ttgctcaaat gggcccagca aacgggcatt tttctcgtat tacccgccct
      901 cgctgtactt tcgatttttt ttcgtcgacc cgtaaaagct tgggctctta aattcgccca
      961 agtcgccgtg ccctatggca ttgcgctctt tgtgttcgtg tttctattct gggccgttta
     1021 catcgtacac gaatactacg ctctaccctt cgacgtaggg cgtgcactgg cgacaggtat
     1081 cgcactcgct ctcctcgcag aagtcgtttt aggtaccatt gataagcatc accgactgtt
     1141 ggcgcttgct ctcgcagtat tagtaactgc cgtgtgcggc accgcttatc gcaaaggagt
     1201 ccctccttac ctagcctacg ccgcgcgcat caatgacccc agggcccctg attatttaca
     1261 tggttggaat gaacaagtgt ttccagaacg ccacccgttt gtcgtcatga cagttcgctc
     1321 gcgtggcagt gagctattat acctatataa agccaaagct cgcggctttg tctggtgcac
     1381 tcacaacccg attcacgcgc ctcgcaaata ttggaaagat caaggcgtgc gttgggtcgc
     1441 ctggggcgaa ccttcgcccg accctgctac tcccggactc taccagtgga ccgtacgatc
     1501 aatcgaacaa gaattagaac tggctcgaaa aaacaattgg tcaagcgacg tggacgatca
     1561 ttgggccggt cgtaccatgg ctcagtgggc cgcccatgct agcagcaacg gtaaagatcc
     1621 atgccttgat aatccggacc cccgaagttg gtgagagtta atttccccga ctgcttgcaa
     1681 aagtgaatca agtcacaata gtttcgcaaa ccagggggac atctgatgaa acactctcaa
     1741 cccatcgcca aacttctact tttactcctg ccagttcttc cggttttctt aggagtcgac
     1801 gcctgccatg atccgaccat ttacaacaac atcgatgatc tatctgcgct gttcgaagaa
     1861 gaatcccgct tatacggtga actagacaac gttcgcgtaa cttcaaccca gacgaacaac
     1921 caacagtggc ttccgctcgc agaggccttc gttgaatggt ccgatcatct aaaagtgctt
     1981 tcggaaaaat ccgaagagct tgttcgaaac tacaacgcac tttcgattga acgaaaacac
     2041 ggctacaaag ctcgcatttt agcgctcgaa ccactctacg aaaatttttt tcggcgacct
     2101 attcgatgga ccttgccgta cgaaccttgg acttatcgga tcacttggga aaatagtttc
     2161 gcaattcagc gagcggaagc catgcgccct catttcggtg tcttgtacaa tcgtgccaac
     2221 gacaccccgt cgttgaactg gtcgatcaac atggttcaag actccaaccc gagcggtctc
     2281 gccaccgccg ttcggaacgc atggaaggcc agacccgcgc ttctcatttg cactggaagt
     2341 tcttcgctag cgtgtggatt gaaatctcag atcgctactt attttagttc tcacttgaat
     2401 cgccagttac aaattcaaaa cgacgactgg atcttttcgg agacctttca accaaattta
     2461 atttttgaaa aattaacgcc aaaccccgcg gatccttatt tggtgtacgg ggaaccagtc
     2521 gccattcgtg tcgctgatac tcaacaatat ttgttcgcct cagaaactgc accaggtgat
     2581 tttaccgttt ctacccaaac cttagttggc ggtggcgttt ggccatggca gtgggaattg
     2641 cacccgattt gggaacgcaa ttcgaatcga actgtacagg cggtcgtcgg tttcggtcta
     2701 tataaccgcg taacccaaat gtatttggtg gactgtccta gatctgcgaa aacaattgct
     2761 tggtaccaag gctcactcga cagtcgaaat atgacagact tataccagtg tcagtagcct
     2821 aattttttcg ctgcaatacg aaggtttcgg tatttcgcaa ctctccgcgt cggagtctat
     2881 aaacattgcc ggcaacggca tctaattcgc gcagcgtgta gacctgataa taaacggtgt
     2941 cgcttcgggg cgaaccggca gccgactcaa cggtaatcca caaggcgccg gcttttctgc
     3001 gtaactccga aattcgaatc ggaagattga gtaacaaacc ctgtcctggt cgcgccattt
     3061 ccacatagat tctgccttgc tggcgctcgt tcactacttc gatattcatt tctaaataca
     3121 tgggcgcgaa tcgaacattg tcggaggtcg aagttgcctt accctcgaaa attccccgaa
     3181 agtcccgtaa aaatcgatcg tcccgtacga cgtcatacgt gtcgacctga ggttttgatt
     3241 cctttagagc agcccgattc aaaaactggg gtacctcatt taagtcagtt acttcattcg
     3301 aatcgtttat tgtcttagag atcggctcga cttcgggaga gcgcatcggc tcttcatggg
     3361 atatcgtggt gactgatggt ttactttccg ttaccggatt cacattaggt tgggcacccg
     3421 gagtcagttc gacgcttttg tttatcgcaa gctgtttgga atcttcaaat gggcgattgg
     3481 gtctaagaaa aaaatagaac ccaagcgccg ccaaactcac ggccaaaagt aagaccctca
     3541 ttgagagtgt cctttaaatc gtgtcccagc cgtagccctc gatttcgtag gcatggtcag
     3601 caagcgactt aataagttcg tcgtcattat tgtcgatttt tccaaaatta cccttcgtgc
     3661 gacgccgaac ttgtcctgaa aaaaccgtaa gtatttcgat ttccttggta cagttcgcga
     3721 ttccttttgc ggccaccgaa ctgttcaccc gacttagcgt acaggccaat acaaagaggt
     3781 ctatcatgat gtcggccagg cgtttcgtag caaattgttt accaataata ttcttgccgt
     3841 gtttgcgtaa aattcgatct gcagcggccg acaaatctcg cacgccttct tcgaaaaccg
     3901 tactgtagtc cttaagcgcc ggatgaatct tggtaaaggt ccctttttca ttggccacac
     3961 cggttgcggc acttaagcga cgtcgggcgt agtcggagag aacgccgaat cccttgatag
     4021 gatcatcgaa aatgccgtcg agagatttcg atatttcttt aagctgttta ccaacgtcgt
     4081 tcatcgccgt tagcgcgata aaaagcctca agatgtcatt ggtaccttcg aagattcggt
     4141 tcactcggca atcgcgcacg atgcgttcgt agggaaactc gcacatgtaa ccgttaccgc
     4201 ctgcaatttg caaaccttcg tcagctgtgc gccacaagca ctctgtcgcg aatactttgc
     4261 tgatagcggc ttcgactgcg tagtcttcgt agccctgatc gacgaggccc gcgaccatgt
     4321 ttaccgcagc ttcggacgcg tagcactcaa caatcatatg accgattttt tgtttcacca
     4381 aaccatactc agcaattggt tttccgaatt gagtacgctc tttggcctgt ttagttgcct
     4441 cggtgattac gtgtttcatg gcccctacgc agcctccgcc caaacccgtt ctcccgctat
     4501 tgagcacttt cattgcgacc ttgaaccctt ttccgggttc acccaaaata tgttcatccg
     4561 aaagccgcac gttttcaaaa aacacggtag ttgtggaact tgcgcgaaga cccattttat
     4621 cttcgtgagg cccaacactc acgcccggca tatcgcgcgt tacgataaac gcggtcattt
     4681 gcccgccctc ttctttttca gtacgcgcaa acacggtaaa aaattgagca attccaccgt
     4741 tggtaatcca aagtttttcg ccgtttagta cccactcttt accttcgcgc actgcttttg
     4801 ttttgatcga cgcagcgtcg gaaccagccc ctggttcggt caaacaaaag gccgcgatca
     4861 tttcaccact ggcgagttta ggcatgtatt tattcttttg ctcgggagtt ccaaagagca
     4921 gcagacctcg cataccaatc gaactatgcg cgcccgcggt aattgcgacc gaaccatcgt
     4981 gtcgcgccac ctgctgcaag acccgagaat aggcggttgc acccagtccg agtccaccat
     5041 gctcctcggg aataatcacg ccgaagagac ccatttgttt catttcttcg atgaactccg
     5101 ggggaagctc agcctggcga tcccattttc gaaaatcggg gccgcgatct ttaagccagt
     5161 tatcaattgt gggaagaatg tctttaaccg ttccgagttc gttttcgttg agttgaggga
     5221 acgggataat gagttcctct tcaatctcac ccatacacaa cgatcgcatg aaactgatct
     5281 gtttattgtc tgacatgctg ttcccccaag taagaaaatg actaactaca gggtaacaca
     5341 aataagactc gccacaacca ggttggtgac tggaaaacaa tccttcaatg ccgcaatgcg
     5401 atagactagg gccttaaatg gaccctcggt actcagctgg cgcttgagcc agaagactcg
     5461 gaactggctg cagtggaaac tgcagactcg ctcgtcgatg cagccggtgc atccgtcggt
     5521 agtgctctag gttttggaat cacaagcggc ttgcttggag tctgaggtcg gggttgtctt
     5581 ggagggcgac catctttatt ttgggctcgg cgctctgaac ggtcaccacc gcgaccaccg
     5641 cgctccccac gcttgccacc acgaccacct tttcctttgc cacgaccacc tttgcgatca
     5701 ccgtcaaacc gaccgcgacc tttttttcgt tcttgtggtg gagccgggaa aagttccagt
     5761 acgtaggtct gctcttcgcg tttttcagaa ccctgtggcg ccttttcggt ttcaagaagc
     5821 gtcattacaa ccacgcgttt tactcgagaa ctctcgccgt caaaaccgcg agcctttgcc
     5881 attt
//
LOCUS       DAOMXO010000048         5842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7258_length_5842_cov_4.755141, whole genome shotgun sequence.
ACCESSION   DAOMXO010000048 DAOMXO010000000
VERSION     DAOMXO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7258_length_5842_cov_4.755141"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /gene="nuoL"
                     /locus_tag="PLH57_01835"
     CDS             complement(<1..1312)
                     /gene="nuoL"
                     /locus_tag="PLH57_01835"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRK01380.1"
                     /translation="MLALILALPLVGFLINGVFGRRFSAKIAGSIASAAILGAFVISV
                     SKFFELYSMDESSRVIEQVVFQWITVGDFSLPFVLRLDPLSSIMTLVVTGVGFLIHVY
                     SIGYMDHDRTPPKFFAYLNLFSFAMLALVLGGNLPIMFLGWEGVGLCSYLLIGYWYED
                     DAKASAGKKAFIVNRIGDLGFLVGIFTLFGLFGTLDFVAIKDVAAQNATTVSIATLTF
                     ATVCLFIGAMGKSAQFPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMIARMNFLFAL
                     CPTTLMIVSGVGAFTALFAATIALAQNDIKKVLAYSTVSQLGYMFMAVGVGAYVAGVF
                     HLVTHAFFKALLFLGSGSVIHGMHEEQDIMKMGGLKKYMPKTFLTFAIGTIAIAGIPP
                     FAGFFSKDEILWNAFSSHHGLAGTALWGVGALTAFLTAFYMTRLT"
     gene            complement(1316..1615)
                     /gene="nuoK"
                     /locus_tag="PLH57_01840"
     CDS             complement(1316..1615)
                     /gene="nuoK"
                     /locus_tag="PLH57_01840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRK01381.1"
                     /translation="MTTTSTLLVASGFFFIGLFGVLVRRNILIVLMSIELMLNAVNLT
                     FAAFSRELEQAAGQMAVFFVITVAAAESAVGLGLLITLFRNLKTVNTDQIQALKE"
     gene            complement(1628..2149)
                     /locus_tag="PLH57_01845"
     CDS             complement(1628..2149)
                     /locus_tag="PLH57_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRK01382.1"
                     /translation="MAATDVFFYIFSAIGLLFALKVVMGKNPISSAFSLVLVFFCMAA
                     NFVLLEAHFIAAMQILVYAGALMVLFVFVIMLLNADVKSLDVGKKPLHYALGGAVCLA
                     LMALVVYALVNGTSFSEKAGLSAEKIDSIGGNAQVVSLMMFSDYILPFELTSILLLVG
                     IVGSVALAKRRTE"
     gene            complement(2149..2598)
                     /gene="nuoI"
                     /locus_tag="PLH57_01850"
     CDS             complement(2149..2598)
                     /gene="nuoI"
                     /locus_tag="PLH57_01850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRK01383.1"
                     /translation="MLVKPKTGLLNKFYFFEITTGLWITMKNFFFGKKVTIQYPEEKR
                     NYSKRFRGLHYIKTVNRVENCTACMLCPTVCPAECIHIEAGERPDKEKYPVIFDIDIL
                     RCCYCGFCEEACPKDAIKLSTIYEMAEHHREKGVYTKASLLVQRGEK"
     gene            complement(2611..3693)
                     /gene="nuoH"
                     /locus_tag="PLH57_01855"
     CDS             complement(2611..3693)
                     /gene="nuoH"
                     /locus_tag="PLH57_01855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRK01384.1"
                     /translation="MGVKTGLVVLVMLQVVPIMVWIERRGSALMQNRLGPNRVGPLGL
                     LQSLADVVKFLFKEDFVPKHVNKFYYLLAPAILLIPAFMTFAVVPFGGSIQIGDRLVH
                     LQIADLNVGIIYILSIASLGVYGIIMAGWSSNNKYSLLGSLRSSAQMISYELPLGLSL
                     VGVLMVFESVNLREIAAAQATPWLFGLPKWGIFVQPVGFLIFMTASFAETNRLPFDLP
                     EGESEIVAGYHLEYGSMKFALFFMAEYMNMVTASALMSTLYFGGYQMLPGMSYLVGPL
                     ASFIGSFDFSFLSLVTPTDCARVLLEVASFTAKVGFFMWFFVWVRWTLPRFRFDQLMD
                     IGWKVLLPLSLANVAVSALLIYFGVI"
     gene            complement(3720..5135)
                     /locus_tag="PLH57_01860"
     CDS             complement(3720..5135)
                     /locus_tag="PLH57_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRK01385.1"
                     /translation="MPKCIIDSKEYEFKAGETVIQIADRNGVDIPRFCYHPALTVVAQ
                     CRMCAVEVDKMGKLQTACSLPATDGMVVHTKSEKVKQAQASVMEFLLINHPLDCPVCD
                     EAGECQLQDYSFSYGAPDSRYDEMRRTYMDLDMGSVIKKNMNRCIHCTRCIRFCDEVG
                     GYREMVALKRGNSTEIVTVDGAPLQTQYAGNLADICPTGSLTLKDFRFKKRAWFLKKT
                     PSVCEGCSQGCNIEVHQENNVVYRTHPRENMDINKWWICDEGRFLFHYVHSEERVLNP
                     SVRDGTSALEKMDWYDAVEKVSPKIKGKALVLVGSDLTQEEIQAIQQFAASKLQTTAV
                     YHYGTENILKAADDTEEDKILRRTSKTSNLHGAEKLGLKPWKSADHNSGEFDCALVFR
                     GGRAMVLNQLRKCVKGAIVGVGVFNSGAVDSYDAVLPGLSFVEKSGTIVNWQGKEQRL
                     NQSIAPLGESKSITDILQLLR"
     gene            complement(5120..>5842)
                     /locus_tag="PLH57_01865"
     CDS             complement(5120..>5842)
                     /locus_tag="PLH57_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRK01386.1"
                     /translation="KRGEPRVKPPFPAIKGAFGGPTIVNNVETLAAIPWIINNGGEAY
                     AKMGKFPNSGGTRLYCVSGHVNKPGLYEFPCGAVTLRELIYEHCGGIRDNRKLKAVIP
                     GGSSAPVLAAHEIDIPLDVEPMAKAGTMLGSCGVIVIDDSWCAVKIIKRVTHFYAHES
                     CGQCTPCREGTRWMEDVLHRIEDGHGKMSDLDLLLNVADNINGKTLCALGDAAAGPVL
                     SFVKKFRHEFEAHIKGGKCLNA"
BASE COUNT         1729 a         1486 c         1202 g         1425 t
ORIGIN      
        1 aagtaaggcg cgtcatgtag aatgctgtta aaaaggcagt cagtgcacca acgccccaga
       61 gcgcagttcc agctaatcca tggtgagagg aaaatgcatt ccacagaatt tcgtctttgc
      121 taaaaaatcc ggcaaacggg ggaattcctg caattgcgat agtaccaatc gcaaaagtca
      181 gaaatgtttt cggcatgtat ttttttagcc cacccatttt catgatgtct tgttcttcgt
      241 gcattccgtg aatcacggag ccggagccaa gaaataaaag cgctttaaaa aaggcatggg
      301 ttactaaatg aaacactccc gccacatatg cacctacccc gaccgccatg aacatgtagc
      361 ccaattggct gacagtcgag taggcgagaa cctttttaat gtcattttgc gcgagtgcga
      421 tcgtcgcagc aaaaagcgcg gtaaatgccc cgaccccact aacaatcatt agggtagtgg
      481 ggcagagcgc gaagagaaag ttcatgcgtg ctatcatata gacgcccgcg gttaccattg
      541 tggcagcatg aattaacgca ctgaccggag tcggacccgc cattgcatcg ggcaaccaaa
      601 catagagcgg aaactgagct gacttgccca tggcacctat aaaaagacac acagtggcga
      661 aggttaacgt tgcaatagaa actgtagtcg cgttttgggc agcaacatct tttatcgcca
      721 caaagtcgag agttccgaaa agaccaaata acgtaaatat accgaccaaa aaccctaaat
      781 caccaattcg gtttacgatg aaggcttttt tccctgctga cgcctttgca tcgtcttcgt
      841 accaatagcc aataagaaga taagaacaga gacccacgcc ctcccaacct aaaaacatga
      901 ttggtaaatt tccgcccaat acgagcgcaa gcatggcgaa gctgaataaa tttaaataag
      961 caaaaaactt tggcggagtg cggtcgtgat ccatgtatcc gatcgaataa acatgaatca
     1021 aaaatcccac ccctgtcaca actaaggtca tgatcgacga aagcggatca agcctcaaaa
     1081 caaaaggcaa agagaaatct cccacagtga tccattgaaa caccacctgt tcgataactc
     1141 tcgagctttc atccattgaa taaagctcaa aaaactttga taccgaaatt acgaaggcac
     1201 caaggatagc tgcggaagcg attgagccag ctatcttcgc agaaaaccgc cgaccaaaaa
     1261 cgccgttaat tagaaaccct accaacggca atgctaagat caacgccaac atcgtttact
     1321 ccttcaacgc ctgaatttgg tccgtattca cggtcttaag atttctaaaa agtgtgatca
     1381 aaaggcctag cccaacggcc gactctgccg cagcaactgt aatcacaaaa aataccgcca
     1441 tctgaccggc cgcctgttcg agctctctcg aaaaggcagc aaaggtaagg ttcacggcat
     1501 tcagcattag ctcaatggac attaaaacta ttagaatgtt tcttcttaca agaactccga
     1561 aaagcccgat aaaaaagaac ccacttgcta ctaataaagt gcttgtcgtc gtcacagtct
     1621 acactcccta ttcggtgcgg cgttttgcaa gcgccaccga gccaactatg ccaacgagca
     1681 acaatattga ggtgagctcg aacggcagta tataatcaga aaacatcatt aacgacacga
     1741 cctgggcgtt cccacctatt gaatcgattt tttccgcaga aagaccggct ttttcggaaa
     1801 aagacgttcc gttgactaac gcgtaaacca ccaaggccat caaggcgagg caaacggcgc
     1861 cccccaatgc gtagtgcaaa ggtttttttc cgacatccag cgatttaacg tcggcgttta
     1921 acaacataat cacgaagaca aaaagaacca tgagcgcgcc ggcgtagacc aaaatttgca
     1981 ttgcggcgat aaaatgagcc tctaacaata cgaaattggc cgccatacaa aaaaagacca
     2041 gcaccaagct aaaggctgaa ctaatgggat ttttacccat tacaaccttc aaggcgaaca
     2101 aaagcccaat ggcagaaaat atataaaaga atacgtcggt cgcggccatt atttctcacc
     2161 ccgctgaact aatagagaag ctttggtgta aacgcccttc tctctatgat gctctgccat
     2221 ttcgtaaatc gtgctaagtt taatggcgtc ctttggacaa gcctcttcac aaaaaccgca
     2281 ataacagcat cgtaaaatat cgatatcaaa tattactgga tacttttctt tgtcgggacg
     2341 ttcaccggct tcgatgtgaa tacactctgc cgggcaaacc gtagggcaca acatgcaagc
     2401 ggtacaattc tccaccctat taacagtttt aatatagtga agaccgcgaa aacgtttcga
     2461 ataattccgc ttttcttccg gatattgaat cgtaactttt tttccgaaga aaaaattctt
     2521 catcgtgatc cacaggcctg tcgtgatctc aaaaaagtag aatttattta aaaggcccgt
     2581 tttgggttta accaacatat aaatatgtcc tcaaataaca ccaaaataaa ttaacaaagc
     2641 ggaaacagcc acattcgcca gcgacaaagg aagcaatacc ttccatccaa tatccatcaa
     2701 ctggtcaaag cgaaaccgag gcaacgtcca ccgtacccaa acaaagaacc acatgaagaa
     2761 gccaactttt gctgtgaaag aagccacctc gagaagtact cgagcgcagt ccgtgggcgt
     2821 taccaatgat aaaaagctaa aatcaaacga tccaataaac gatgcaagag gtccgaccaa
     2881 ataggacatc cccggaagca tttggtagcc accaaaatag agggtgctca taagagccga
     2941 agcggtgacc atgttcatgt attccgccat gaagaataga gcaaatttca tgcttccgta
     3001 ttctaggtgg tagcccgcaa cgatttcact ttcaccctcg ggtaaatcga acgggagtcg
     3061 gttggtctct gcgaaagaag ctgtcataaa aataagaaat ccaacgggct gaacaaaaat
     3121 gccccacttc ggtagtccaa acaaccatgg ggtcgcctga gctgcagcga tttcccgcaa
     3181 atttacggac tcgaaaacca tgagtacgcc gactaaactt aaacccaatg gcaactcata
     3241 actaatcatt tgtgccgaac tgcgtaggct gcctaaaagg gaatatttat tatttgaaga
     3301 ccagccagcc attataatgc cgtagacacc caacgaagcg atcgaaagaa tataaataat
     3361 tccgacgttg agatcagcta tttgcaaatg tacgaggcga tcaccaattt gaattgaacc
     3421 gccaaacggc acgactgcga acgtcataaa cgcaggaatc aacaaaatcg caggcgccaa
     3481 caaataataa aatttgttga cgtgtttagg aacaaaatcc tctttaaaaa gaaacttcac
     3541 cacgtcggcg agcgactgta atagacccag gggaccaact cggtttggtc cgagtcgatt
     3601 ttgcatcaaa gccgatccgc gtctttcgat ccaaaccatg atcggcacaa cctgaagcat
     3661 gaccaagact acgaggccgg tcttaactcc cattgcgatc cactcaaatc tatccacgtt
     3721 tacctcaata gttgcaaaat atcagtgatc gacttcgact caccgagcgg cgcgattgac
     3781 tgattcaggc gttgttcttt tccctgccaa ttaacgatgg ttccgctttt ttctacaaaa
     3841 gacagaccgg gcaacacggc gtcgtaactg tcaacagctc ctgaattaaa cacacctacg
     3901 ccgacgattg cgccctttac gcattttcga agctgattca agaccatcgc cctgccacct
     3961 cgaaacacta acgcacaatc aaattctccg ctgttatggt cggctgattt ccagggtttt
     4021 agacccagct tttctgcccc gtgcaggtta ctcgttttac tggtgcgcct taggatttta
     4081 tcttcttcag tgtcatcggc ggcctttaaa atattttcgg ttccgtagtg ataaactgcg
     4141 gtcgtttgta acttagaggc ggcaaattgt tgaattgctt gaatctcttc ttgcgtgaga
     4201 tccgagccca ctaaaacgag tgctttgcct tttatttttg gagaaacttt ctcgactgcg
     4261 tcgtaccagt ccattttttc caacgccgac gtaccgtctc gtactgaggg atttaatacg
     4321 cgctcttccg agtgtacgta gtgaaataga aatcggcctt catcgcagat ccaccactta
     4381 ttaatatcca tgttttcccg cgggtgagtg cgatacacga cattattttc ttgatgaact
     4441 tcgatgttac aaccttgaga acagccttcg catacactgg gggttttctt taaaaaccag
     4501 gctcgttttt taaatcgaaa atctttaagt gtaagcgaac ccgtcgggca gatatccgct
     4561 aaattaccgg catattgcgt ttgtagcgga gccccatcaa cagtaacaat ctctgtagaa
     4621 ttaccccgct ttaaagctac catttcacga tatccgccga cttcgtcgca aaatcgaata
     4681 caacgagtgc agtgaatgca gcggttcata ttcttcttaa tgaccgaccc catatcaagg
     4741 tccatgtacg tacgccgcat ttcatcgtat cgagaatcgg gtgctccgta cgaaaagcta
     4801 taatcttgaa gctgacactc tccggcctca tcacaaactg ggcagtcgag cgggtgattg
     4861 ataagtaaaa actccattac tgaggcctga gcctgcttaa ccttttcaga cttagtatga
     4921 acaaccatgc cgtcggttgc gggcaatgaa caggctgttt gaagttttcc cattttatca
     4981 acttcgactg cgcacattct gcactgggcc actacggtga gcgccgggtg ataacaaaat
     5041 ctcggaatgt cgacaccatt tcgatcggca atttgaataa ccgtttcacc ggccttaaac
     5101 tcatattctt tgctgtcaat tatgcattta ggcattttcc acccttaatg tgcgcttcaa
     5161 attcatgccg gaattttttc acaaaactta atactggtcc ggcagcagcg tctcccagtg
     5221 cacaaagcgt ttttccgtta atattgtccg ctacgtttaa aagcagatcg aggtcagaca
     5281 ttttcccatg tccatcttcg atacggtgca atacatcttc catccaacgc gtgccctcac
     5341 ggcacggcgt gcactgacca caggattcgt gtgcataaaa gtgggtcact cgtttgataa
     5401 tttttaccgc acaccatgaa tcatcgataa caattacacc gcacgacccg agcatggtgc
     5461 ccgctttagc cattggctct acgtctagtg gaatgtcgat ttcgtgtgcg gccaatactg
     5521 gcgccgacga tccgcccgga attactgctt tgagttttcg gttatctcga atgccaccac
     5581 aatgttcgta gatcagctct cgtaaagtga ctgcgccaca agggaattcg tagagccccg
     5641 gtttgtttac gtgtccactc acacagtaaa gccgggtccc accgctattg gggaatttac
     5701 ccatttttgc gtaggcctcg cccccattgt taatgatcca aggaatcgcc gccaatgttt
     5761 caacattgtt aacaatagtt ggaccaccaa acgcaccttt aatagccgga aatggcggtt
     5821 ttactctcgg ctcccccctt tt
//
LOCUS       DAOMXO010000049         5782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7360_length_5782_cov_4.522787, whole genome shotgun sequence.
ACCESSION   DAOMXO010000049 DAOMXO010000000
VERSION     DAOMXO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7360_length_5782_cov_4.522787"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="PLH57_01870"
     CDS             complement(<1..1508)
                     /locus_tag="PLH57_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRK01387.1"
                     /translation="MWHVKTAIVTFLISSILMAADLKVEPSATEISEDDSVSVQFEIS
                     QEGNEGESGDIFFDADDFEELNVMRSQGMQSLWINGNFQTKRTLTVTVVLHPKRTGEL
                     KIQNIRTTVGNKKLTAPDQTISVLDRDAYAKKFGNGGSLPGYGSAIGRRHPAPRGSNI
                     GARESGGNLLVRTEPSKTKVYKGEQILLTYALYSQVTVASVGAERYPNAPGFLKEEIE
                     MPILKRLQFEPAVLGGKAFKRAVLAQYAIYPVKTGKLTIDPLTTKVNVRSMRGIFDQD
                     DEDIFGMNQFFKALQGQVVTRSSDRVDVEVLPLPTEGQPVDFSGLVGDFTIAISVDKT
                     ALKVGEALNVKVKVEGDGHAAILEKLPISWPEQFELYEDKARTLFHKTGRTERTFDFM
                     LIPKTKGQFEVPAIKISMFNPTSARYETRQTKPISIEVIEGSGGLVFHPTKKEQNTGP
                     VLDVRGWMSPDRNRRSVVPWILGTLVFVGGLGLFLWSLLPFILRRNYEIARA"
     gene            complement(1511..3277)
                     /locus_tag="PLH57_01875"
     CDS             complement(1511..3277)
                     /locus_tag="PLH57_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRK01388.1"
                     /translation="MPANLWLMLPWLVCAFLWLMFEQRWKKRIEKFAAPEALRALIRD
                     TRGQSQDLSRARLRQMVVRWLSGALVIVALARPQLGSRVETVKTQGLDVVFALDVSRS
                     MLVEDVVPSRLKKAKHIIRSFLDRLGGDRVGVVVFAGSAYPAIPLTSDFDFIVKTLEG
                     IDDRAVPNQGSDLALGLSQAMRLINNGGVNEKNDNNTGEASGRLVVVLSDGEEHEGNE
                     SKTADRLAKDSIKVYSIGVGSERGGPIPIRDDNGSLRGYKKDESGNIVQSKLGKANLE
                     AVAKKTGGQYYTASTNEGEIDDILSNVSPGGSGSYRQVVVYRELFQYPIGMAVALLLI
                     QMLAAVLRYSSKNLSPTLGMLLVALSVFGAKNVQASSLREYSETQKGVDSYSKKKYSD
                     AIDRFTRAQAASPESDLQHLNLGGSLYQAGDLDGAIREFDALAKSRDANFAAKGAFNL
                     GQTFEKKKDREKAMQAYQNGLNTLARAEKPDPETLLRLKQALESAQQRQNQQSQEGNE
                     GESDPKKSENGESQDSEKKDSKGKVDEKSVGKKQKFKGERLSEMDAKQLLERLQDQEK
                     KTQQRLMRGKSEKPNNAVGKDW"
     gene            complement(3286..4281)
                     /locus_tag="PLH57_01880"
     CDS             complement(3286..4281)
                     /locus_tag="PLH57_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRK01389.1"
                     /translation="MIFRDKEVLLIAIVLVYVLARASRGTAPTKLLVSSARFWNVHVG
                     KRLSHRLIQAIIWTMGILFLLFALARPQVPHSNIKRTVEGIDIMMVLDLSASMRVEDF
                     RDRNRLEMAKPILKEFIRGRASDRIGFEIFSGEAVTVVPPTLDYELLLNAIDGVEIGD
                     LKDGTAIGDALATAVSRLKESTAKTRVVILVTDGDSNIGQVDPLTAGELAKGFGIKVY
                     SIAMGRDGRVAMPFVTKDFLGRSVKTYQYYESSINPELLKQISALTGGKFYRVEGDEK
                     SFRQVFQEIDRLEKTAVEKTEHVQYEEKFARYAWIGLIALLFFILSEATIFRVFP"
     gene            complement(4278..5132)
                     /locus_tag="PLH57_01885"
     CDS             complement(4278..5132)
                     /locus_tag="PLH57_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01390.1"
                     /translation="MEIEKATTTVKVLAAPFGKPTAGDITELVVKIPEAFLGNETAVI
                     EPEFAESLALGPVKLGENIRVLTWSRREDTVVLQLTTYVPSKIDIPSVAFRAEGKVIF
                     VSEPQTIEYGTVGQSEKQLEIYHPQSAALPQWVWLGLSFATLIFVVGCIIFIARWYRR
                     KQQKLRDLAAIPRVLTPHEALLEAVNSLNKKNYLINRRFKAHYFALSDAAKKFLADKL
                     RIDAEEKTTRELHRELQRVLLDHSLADDWRGIFEELDIVKFTDQQPVDLQASDIANRI
                     LKLAERTS"
     gene            complement(5145..>5782)
                     /locus_tag="PLH57_01890"
     CDS             complement(5145..>5782)
                     /locus_tag="PLH57_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRK01391.1"
                     /translation="LVVDVSGSGLFSTVAKNKTEILAETCAMLAFAAVRNNDRVGLIL
                     FSDRIEKHVPPKKGRSHAMRLVTEILYYTPVGRKTNISSALEHCANVMKHTAVVIVAS
                     DFMDSGYENALKRVAKRHDVIAMQARDRRDFEVPPIGEIELIDPETGESFLANTASYQ
                     FQKEFKDRVTQFNKQTDDIFARTGVDRVEIWTHEDTFMKIANYFKSRSKRI"
BASE COUNT         1431 a         1474 c         1182 g         1695 t
ORIGIN      
        1 tttgctcggg cgatttcgta gtttctccgt aaaataaagg gcaaaagact ccagagaaaa
       61 aggccgagcc caccgacaaa aaccaaagta ccaaggatcc aaggtacaac ggaccggcgg
      121 ttacgatctg gcgacatcca cccacgaacg tccaggacag gtcctgtatt ttgttccttt
      181 ttggtcggat gaaaaaccag cccccccgaa ccctcgataa cctctatgga aattggttta
      241 gtttgccgcg tttcgtaacg agctgaagtt ggattgaaca tcgatatttt aatcgcaggc
      301 acttcaaatt gccccttggt cttgggaatc aacatgaaat caaaagtacg ctccgtacgg
      361 ccagttttat gaaacagagt tcgcgcctta tcctcataca attcgaattg ctcgggccaa
      421 ctaatcggaa gtttttctaa aattgccgcg tgcccatctc cctcgacctt caccttaacg
      481 ttcagggctt ctcccacttt caaagcggtt ttatctacac ttatcgcaat cgtaaaatcg
      541 ccgactaacc ctgaaaaatc cacgggctgc ccctcggtgg gcaacggcaa gacttcaacg
      601 tccacacggt cactcgaacg ggtcacgacc tgcccttgca acgctttaaa aaattggttc
      661 attccaaaga tgtcttcgtc gtcttgatcg aaaatacccc gcatcgaacg tacattcact
      721 ttggttgtca gtggatcgat ggtcaactta ccagtcttaa ccgggtagat ggcgtattga
      781 gccaacacag cgcgcttaaa ggcctttcca ccgagcactg cgggctcaaa ctggagtcgc
      841 tttaatattg gcatttctat ttcttctttt aaaaatcctg gagcattcgg gtatcgttcc
      901 gcaccaactg aagcgacagt tacttgcgag taaagcgcat aggttaatag gatttgctcg
      961 cccttatata ctttggtctt acttggttcg gtgcgaacca ataaattgcc gccactctct
     1021 cgtgcgccaa tgttagaacc tcttggagct gggtggcgtc gccctatcgc agaaccgtaa
     1081 cccggaagcg aacctccgtt accgaatttt ttggcatagg catctcgatc taatacagaa
     1141 atggtctgat cgggagccgt aagtttttta ttcccgactg tagtgcgaat attttgaatt
     1201 tttaattcac cagtccgttt ggggtgcaat actactgtga ctgtcaacgt gcgtttcgtt
     1261 tgaaaatttc catttatcca aagcgattgc atgccttggg aacgcattac attcaattct
     1321 tcgaagtcgt ctgcgtcaaa aaaaatatcg cctgactcac cttcgttacc ttcttgggaa
     1381 atttcaaatt gaaccgaaac actgtcatct tcggaaattt cagtggcact cggttcaact
     1441 tttaaatccg ccgccatcaa tatcgagcta attaaaaaag ttacgatcgc tgttttaacg
     1501 tgccacatat ttaccaatct tttccaaccg cgttgttcgg tttttcgctt ttaccccgca
     1561 tcaaacgttg ttgagttttc ttttcctgat cctgaagtct ttctaatagt tgttttgcgt
     1621 ccatctccga aagacgctct cctttgaact tttgtttttt tccgacactt ttttcatcta
     1681 cttttccctt cgaatccttt ttttctgagt cctgagattc gccgttctca gattttttcg
     1741 gatccgattc gccttcgttg ccttcttgcg actgctggtt ttgacgttgt tgtgctgact
     1801 ctaacgcctg tttaagccgc aaaagtgtct ccggatcagg tttttccgcc ctagccagcg
     1861 tattcaaacc attttgatag gcttgcatag ctttttctcg atcctttttc ttttcaaagg
     1921 tctgtcccaa attaaacgca cccttcgccg cgaaattagc atctctactt tttgctaatg
     1981 cgtcgaactc tctaatggct ccatcaagat cgcccgcttg ataaagtgat ccgccgagat
     2041 tcagatgttg taagtcgctc tcagggcttg ccgcttgcgc tcgcgtaaaa cgatcgatcg
     2101 cgtcgctgta tttttttttg gaatacgaat cgacgccctt ttgcgtttcc gaatattctc
     2161 gcaacgaact tgcctggaca ttttttgcgc caaacacact taatgcgacc aaaagcatcc
     2221 ctaatgtggg actcagattt tttgaagaat atctcaaaac cgccgcgagc atttgaatca
     2281 aaagcagcgc cactgccatt cctatcgggt actggaatag ttcgcgatac acaactacct
     2341 gtcgataact accgctgccc cccggtgaaa cattgctgag tatgtcgtca atctcacctt
     2401 catttgtcga tgcagtgtaa tactgaccgc cggttttctt tgcaactgct tcaagattcg
     2461 cttttccgag tttagattgg acaatattac cactttcgtc ctttttgtaa ccgcgaagag
     2521 agccgttatc atcgcgaatt ggaatgggcc caccgcgctc actacctacg ccgatggaat
     2581 atactttgat cgaatcttta gctagtctgt cggcggtttt actctcattt ccttcgtgtt
     2641 cttccccatc gcttaagacg accacgagcc gtccgctagc ttcaccggtg ttgttatcgt
     2701 ttttttcgtt tactccaccg ttgtttatta accgcattgc ttgagagagc ccgagtgcta
     2761 gatcactacc ttgattcgga accgctcggt catcgatacc ttcgagagtc ttaacaataa
     2821 aatcaaaatc actggttagg ggaatggcgg ggtatgctga gccagcaaag acaacgactc
     2881 ctacgcgatc acctccgaga cgatccaaaa aacttcgaat tatatgtttg gcttttttta
     2941 gacgactcgg gacgacatct tccacgagca tacttcttga aacatctagt gcaaatacca
     3001 cgtcaagtcc ctgggttttc actgtctcaa cccgactgcc aagctgcgga cgagccagcg
     3061 ccacgataac cagggctcca ctcaaccacc gcaccaccat ttgccgcaat ctcgcccgcg
     3121 acagatcttg cgactggccc ctggtgtccc taatgagcgc ccgtaatgcc tcgggggccg
     3181 caaacttttc aattctcttc ttccaccttt gttcgaacat tagccaaaga aacgcgcata
     3241 ccaaccaagg gagcattagc cacaaattcg ctggcattaa gaatctcatg gaaacacccg
     3301 gaaaatagtt gcttcactta gaataaaaaa taataacgcg attaaaccaa tccaagcgta
     3361 tcgcgcaaat ttttcttcgt attgtacgtg ctctgttttt tcgactgcag tcttttctag
     3421 acgatcaatt tcctgaaaaa cctgtctaaa tgacttttcg tcaccctcca ctcgatagaa
     3481 tttcccaccc gttagggctg atatctgttt tagcaactcc ggattaatcg agctctcgta
     3541 atactgatag gtttttacgg atcgacccaa aaaatctttg gttacaaatg gcatagccac
     3601 gcgcccgtct cggcccatag caatgctgta gacttttatt ccgaatcctt tagctagttc
     3661 gccggcagtc aatgggtcga cctgtccaat attactatcg ccgtcggtaa cgagaattac
     3721 aactcgggtc ttcgcggtgc tctcttttaa acggctgacc gcggttgcca acgcgtcgcc
     3781 aatcgcagtt ccatccttta gatccccaat ctccacaccg tcgattgcgt tcagtagtaa
     3841 ttcataatct agtgttggcg gtactacggt cacggcctca cccgaaaaga tttcgaaacc
     3901 aatacgatca cttgcccgac cgcgaataaa ttccttcaaa attggcttag ccatttcgag
     3961 tcgattgcga tcccgaaaat cctctacccg catactggcg gaaagatcga gtaccatcat
     4021 gatatcgatt ccttcgaccg tacgctttat gttcgagtgt ggtacctgcg ggcgagccag
     4081 tgcaaatagt aaaaatagta tgcccatcgt ccaaataatt gcttgaatca atcgatgaga
     4141 aagtcgtttg cctacatgga cattccaaaa tcgagcagaa gaaactagca actttgtagg
     4201 cgctgtgccc ctagaagcac gtgccagtac gtatacaagc acgatcgcga tcaacaatac
     4261 ttctttgtcg cgaaatatca tgaagtcctc tcggcaagct ttagtatacg attggcgatg
     4321 tcacttgcct gtaaatcgac gggttgctga tcagtaaatt tcacaatatc taactcttcg
     4381 aaaataccgc gccaatcatc ggccaagctg tgatcaagca gcactctttg caattcgcgg
     4441 tgcagttccc gggtagtttt ttcttccgcg tcgattctaa gtttgtccgc caggaatttt
     4501 tttgcagcgt cacttaacgc aaaataatgc gctttaaagc ggcgattaat taagtaattc
     4561 tttttgttta aagaattaac tgcctccaga agtgcttcgt ggggggttaa cactcgtggt
     4621 atggcggcaa gatctctgag tttttgctgt tttcgtcggt accacctcgc aatgaagata
     4681 atacatccaa caacgaaaat tagtgtcgca aatgaaagac cgagccacac ccactgaggc
     4741 agggcggcgc tttgtggatg ataaatttcc aattgttttt cgctttgacc aactgttccg
     4801 tattctatgg tttgcggttc tgaaacaaat attacttttc cttcggcccg aaacgccacc
     4861 gagggtatgt caattttact tggcacatag gtcgtaagtt gtagaacaac ggtatcttcg
     4921 cgacgactcc aggttaatac ccgaatattt tcacctaatt ttacaggccc gagagccaaa
     4981 gattcggcaa attccggttc gatcaccgcc gtttcattgc ctagaaaggc ttcggggatc
     5041 tttacaacta attccgtaat atcgccggcc gttggttttc cgaagggagc ggccagtact
     5101 tttacagtag ttgtggcttt ttcaatttcc acttagtgcc ctcttcaaat tctttttgat
     5161 ctagacttaa aatagtttgc gattttcatg aaggtgtctt cgtgagtcca aatttctact
     5221 cgatctactc cagtgcgtgc aaaaatatcg tcagtttgtt tattgaattg agtcacgcga
     5281 tctttaaatt ctttttgaaa ctgataggaa gcggtgttgg ccaagaaact ttccccagtt
     5341 tccgggtcta tcaactcgat ttcgccaatg gggggtacct cgaaatctcg ccgatctctg
     5401 gcttgcattg cgataacatc gtgtcgtttt gctacccgct tgagcgcatt ttcgtatccg
     5461 ctatccataa aatcgctggc tacaatgact acggcggtgt gtttcatcac gttcgcgcag
     5521 tgttcaagtg cgctcgatat attagttttt cggccaaccg gtgtataata aagaatttca
     5581 gtcaccaatc gcatcgcatg ggaacggcct tttttgggcg gcacatgttt ttcaattcga
     5641 tcagaaaata gaattaaacc tacacgatcg ttatttcgca cagccgcgaa tgccaacata
     5701 gcgcaagttt cggctaatat ttctgtttta tttttggcaa cagtggaaaa aagtccactt
     5761 ccgctaacat ccaccactag at
//
LOCUS       DAOMXO010000050         5724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7452_length_5724_cov_4.506262, whole genome shotgun sequence.
ACCESSION   DAOMXO010000050 DAOMXO010000000
VERSION     DAOMXO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7452_length_5724_cov_4.506262"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="PLH57_01895"
     CDS             complement(<1..1415)
                     /locus_tag="PLH57_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01392.1"
                     /translation="MSVDSVSSAQREIELAKRLLKEREEDHAREVDKMRQGQDDQVRQ
                     LRESQQEAIQRLNEKSTKFHERQREDFTKQMGKVSDQFEKTLSEERRTNYDRTGRIID
                     DATRQRTIENETRANQIRNLLDANRTTSDTEREQAAGRIKDLNDKYNADREYFARFTD
                     QKLGEMRDANREALAKTTAEARAKIDANTSSSSEFNNREKMGAELRFKRLAEQTKTER
                     DMLKNAGTLREDQLKDDKMLLERKLGADGNRALNQYRERMSENLAKTINDNNVLSAQR
                     EQENAARLARQENDHQAKHQQAVNQFRKNEAEMKYANEQLRNRNDLQRELDDRRHKQD
                     TFLNSEAIRRQEEGARESLKNRFSGRLDEVENKHAGRFREYQNEMQQKMAEMELRGSV
                     ERNTNEQKKAVEFAAQDSKHNQEKLNINKAFEDKILAAEELRQRQLEDLTKNSQETLR
                     EVQKTKDRQIASTNRENQTRL"
     gene            complement(1575..2642)
                     /locus_tag="PLH57_01900"
     CDS             complement(1575..2642)
                     /locus_tag="PLH57_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRK01393.1"
                     /translation="MSVKNTRTTVLGLSLVAAIGIIVACTGQGTAKDKPKYVFKDGSG
                     PNGAILTIDGKPFDQEQLMGDNKGDYAEALKRIYDLNLAGTGRLLLEVGYSEEAKKAG
                     MSVDEYVETKVLKSDIKISDSEYNAFVKEKNIPKEQLNAQLKERIMAYMKGQKRMEQR
                     DALILSLSKKHKVELYFKKPNVRTDIEVGDAPFLGSKDAKVKVVAFSDFECPFCSRAA
                     NIVTQLKKIYGNKVQVAFKHYPLPMHPKAKPASEASMCINEQSSDKFWKFHDKLFENQ
                     DKLDDENLKKYAKEVGANMEKFTECFNSKKYAAHVENDLRYGSQKGVSSTPTFFINGI
                     EVKGAQPIERFRELIDEELAG"
     gene            complement(2753..3922)
                     /locus_tag="PLH57_01905"
     CDS             complement(2753..3922)
                     /locus_tag="PLH57_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010845855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRK01394.1"
                     /translation="MIHFKSPFLLLLIVSVSASATSPSTVALRARDLGIQFDGTPGKQ
                     NSITDVNGTLVGHTTLTIDEGSNAIRTGVTAVLPAGHTQSKVPASYFSLNGNGEMTGT
                     HWISESGFLEGPVLLTNTFSVGLVRDAVRKWGESRFPNNDPDLDDAFMLPVVAETWDG
                     FLSNIKAHAVTEGHVFSALDSANAGPPLEGGVGGGTGMVCFEFKCGIGTSSRVIENYT
                     VGVLVQANFGDRDELLVRGVPVGEQISDLLPDLEQPLNESLAEHSPLARKKDGSIIVI
                     VATDAPLLPSQLNRIAKRATVGLARTGSIAHNSSGDLFLAYSTAVPRTDAEGNELWSA
                     IPNSAMDPLFRATADATEEAILNALVAGRTTIGKDYVRVHGIPHERLLSLLKDRK"
     gene            4015..4560
                     /locus_tag="PLH57_01910"
     CDS             4015..4560
                     /locus_tag="PLH57_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01395.1"
                     /translation="MNQSGRNWKKTAYRAASVVFIAVFGACSTFHQPGKESVKIYSRI
                     YLTDYNTAWQAALEALKPFDRTVQNRQAGLLQTTWIENTAQKNFIDSFGDRPTYMKAK
                     YRLRMSLSPGHYNGRPSVKVSIQKEQMVQRDALEGWNEAVSDSVEENTMLYRVGRIIF
                     SKVKLKKMEEQRIKDVLDQGV"
     gene            complement(4532..5278)
                     /locus_tag="PLH57_01915"
     CDS             complement(4532..5278)
                     /locus_tag="PLH57_01915"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRK01396.1"
                     /translation="MKMTKFNFGAFLGASLMFLASAHASVPKPNKLNSLRSKDLDTAT
                     SLFSSTVQKNRSVSAWIGALHSKFGPKTSDILHEILESNSSSESMRWASLIGVARLNG
                     KKSLETLRTYMSHSSWLLRDAAIKSAVALNARELAPHIESRLQDEALVIRTSAVDAIG
                     QLKLKTSSKKLLAALFDPSNTHGGKALWIHQHILKVLREFRYEAALPKLGEYAKKYPK
                     DIKLRTEVQKTIRALQAKAPNIKRPDPKRL"
     gene            complement(5286..>5724)
                     /locus_tag="PLH57_01920"
     CDS             complement(5286..>5724)
                     /locus_tag="PLH57_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01397.1"
                     /translation="PNTGAPAVSATAPAVSATAPAASAAPAASATAPAASVAPAASAA
                     GNASPIAAAEQAVAENKANDQFEQLVDKVEESLATPAGASAVDPAAVAAAKTAPPASD
                     TAAPPTPAAQATDIKPSAAPVAAAKPDKSDTDLLSEFERMLSG"
BASE COUNT         1223 a         1312 c         1510 g         1679 t
ORIGIN      
        1 aaaagtctgg tctgattttc gcgattagtc gatgcgattt ggcgatcttt cgttttttgt
       61 acttcgcgga gagtttcttg gctattttta gtaagatctt cgagctggcg ttgccttaat
      121 tcttccgctg ctagaatctt gtcttcgaaa gctttgttga tgtttagttt ttcttggttg
      181 tgtttagaat cttgcgccgc aaactctact gcttttttct gttcgttggt attacgctca
      241 acagaacctc tcagttccat ttccgccatt ttttgttgca tttcattctg atattcgcgg
      301 aatcttccgg cgtgtttgtt ttcgacttca tccagtcgac cagagaaacg attttttaag
      361 ctctcccgag cgccttcttc ttgtcgacga atagcttctg agtttaagaa cgtgtcctgt
      421 ttgtgtcggc gatcatcaag ttcccgttgc agatcgttac ggtttcgcag ttgttcgtta
      481 gcatacttca tttcggcttc atttttgcga aactgattca cagcctgctg gtgttttgct
      541 tggtggtcgt tctcttgacg agccaaacgg gcagcgtttt cttgttcccg ttgggcgctg
      601 agaacattgt tgtcgttgat cgttttcgct agattctcgg acatccgttc gcggtattga
      661 tttagcgcac ggttgccgtc ggctccaagt ttacgctcaa gcaacatctt gtcgtcttta
      721 agctgatctt ctctaagagt gccggcgttt ttcagcatgt cacgctcagt tttggtttgt
      781 tcagccagac gtttgaagcg gagctcggcg cccattttct ctcgattatt aaactcagaa
      841 ctactactcg tgttggcatc gatcttggcg cgggcttctg ctgttgtctt agcaagcgct
      901 tcgcggttgg cgtcgcgcat ttcgcctagc ttttggtcgg taaagcgggc gaagtattcg
      961 cgatctgcat tgtatttgtc gtttagatct ttgattcgac cagccgcctg ctcacgttca
     1021 gtgtcggaag tagtgcggtt agcatctaat aggttacgaa tttgattggc ccgagtttcg
     1081 ttttcgatcg ttcgttggcg agtagcatcg tcgatgatcc taccagtgcg atcgtagttt
     1141 gtgcgacgct cttcggacaa ggttttttcg aattgatcag acaccttacc catttgttta
     1201 gtgaagtctt cgcgttgacg ttcgtgaaat tttgtagatt tctcattgag tcgttgaatg
     1261 gcttcttgtt gggattctcg aagttggcgc acttggtcgt cttgaccttg gcgcattttg
     1321 tcgacttcgc gcgcgtggtc ctcttcacgc tctttgagta agcgtttagc tagttcgatt
     1381 tcgcgctggg ctgaagaaac gctgtctact gacatgaggc gatctccctc cttggagatg
     1441 gcgtatcatg ttgattttcg cagaaagaaa atgactaaag aagtcggtaa aaaatagtcg
     1501 gcaattcgcg gcaaaaaaaa aacggcgcga ttttagtcgc gccgttttga atacaggcat
     1561 atttcggaaa aggcttatcc ggcgagttct tcgtcgatca gttcgcggaa gcgttcaatt
     1621 ggttgcgctc ctttgacttc gatgccgtta atgaagaacg tcggagtgct gctcacgcct
     1681 ttttgacttc cgtaacgaag gtcattttca acgtgggcgg cgtacttctt agagttgaag
     1741 cactcggtaa atttttccat gttggcgcca acctcttttg cgtacttctt gaggttttcg
     1801 tcatccagtt tgtcttggtt ctcgaaaagt ttgtcatgaa acttccaaaa cttatcagag
     1861 ctttgttcgt taatacacat ggaggcttcc gaagctggtt ttgctttagg gtgcatagga
     1921 agtggatagt gtttgaatgc cacttgaacc ttgttaccgt aaatcttttt gagttgagtt
     1981 acgatattcg ctgcacgtga acaaaacggg cattcgaagt ccgaaaacgc gactactttt
     2041 actttcgcgt ctttcgagcc gaggaatgga gcgtcgccaa cttcgatgtc ggttcgaacg
     2101 ttcggtttct taaaatacaa ttcaacttta tgttttttgg aaaggcttag gatcaaagca
     2161 tctcgctgct ccatgcgctt ctgacccttc atatacgcca tgatgcgctc tttgagttga
     2221 gcattgagtt gttctttcgg aatgttcttt tctttcacga acgcattgta ttctgaatcc
     2281 gaaattttga tatcagactt taaaaccttg gtttcgacgt attcgtcgac ggacattcca
     2341 gcttttttag cttcttcact gtaacccact tcgagaagca gtcggcctgt gcccgcgagg
     2401 ttgagatcat aaatgcgttt gagggcttcc gcgtagtcgc ccttgttatc gcccatgagc
     2461 tgttcttggt cgaagggttt gccgtcaatc gttaggatgg cgccgttagg acccgatcca
     2521 tctttgaaga cgtatttggg tttatctttt gcggttcctt ggccggtaca tgcgacgata
     2581 atcccgatgg ccgcaacaag gctaagtcca agcaccgtag ttcgagtatt cttgacggac
     2641 attaatgcct ccttctatct ccattgacat gaaaaatttt ggctacgatt gcaaatggta
     2701 gacccccggt aggttattgg caatgaaatt tcaggtcact agacttggcg cattattttc
     2761 ggtctttaag aagtgaaagg agtcgttcgt gagggattcc gtgcacgcgc acgtagtctt
     2821 ttccaattgt cgtgcgacca gcgacgagag cgtttagaat ggcctcttcg gtcgcgtcgg
     2881 cggtggcgcg aaacagggga tccatcgcgg agttcggaat ggcgctccaa agttcattgc
     2941 cttcagcgtc ggtacggggc accgctgtcg agtaggcgag aaacaaatca cccgaggaat
     3001 tgtgagcgat actcccagtg cgtgccagcc caacggtggc acgtttggct attcggttga
     3061 gttgcgacgg caaaagcggc gcgtccgttg cgacaatcac gataatggag ccatcttttt
     3121 ttcgtgctag tggtgagtgt tcggccaaag attcgtttaa aggctgttcg aggtccggca
     3181 aaaggtcgga aatctgttcg ccgacgggga cgccgcgtac gagcaattcg tcgcggtcac
     3241 cgaagtttgc ttgcacgagc actccaaccg tgtagttttc aatcactcgc gaggaagttc
     3301 cgatgccgca tttgaactcg aaacacacca tgcccgttcc gccacctacg ccaccctcaa
     3361 gcggggggcc ggcatttgct gaatccaggg cggaaaaaac gtgtccttca gtgacggcgt
     3421 gggctttgat gttgcttaaa aatccgtccc aggtttcagc caccaccggc aacataaatg
     3481 catcgtcaag atcggggtcg ttgttcggaa atcgcgattc cccccatttg cgcacggcgt
     3541 ctcgaacaag acctacgctg aaagtattcg tcaagagtac cggtccctcg agaaatcctg
     3601 attccgagat ccagtgggta cccgtcattt cgccgtttcc gtttagcgaa aaataggatg
     3661 ccggtacttt gctctgggtg tggccggcgg gaagaactgc ggtgacaccg gtgcgaatgg
     3721 cgtttgagcc ttcgtctatt gtgagggtgg tgtgccccac aagggtgcca tttacgtccg
     3781 ttatagaatt ttgtttccct ggagtgccgt caaattgaat tccgagatct ctggctcgca
     3841 gcgccacggt ggacggactg gtcgctgagg ccgagacgga cacgattaaa agtagcagga
     3901 acggactttt aaaatggatc aagagtttct cccggttagg tttttttggg acataactca
     3961 gggttcgtgc gaaaatctac gaggaagatt tgaggaaatt ttggggtgat tgcattgaat
     4021 caatcgggta gaaactggaa aaaaaccgct taccgcgcag cgtcagttgt ttttatagcg
     4081 gtgtttggag cttgcagtac ctttcatcag cccggcaaag agtccgtgaa gatatattcg
     4141 cgaatttatt tgaccgacta caatactgct tggcaggcgg cccttgaagc actgaagccg
     4201 ttcgatcgca cggtacaaaa ccggcaggca ggtttactgc aaactacttg gatcgaaaac
     4261 accgcccaga agaacttcat cgattctttc ggagatcgac cgacctatat gaaggcaaag
     4321 tatcgacttc gcatgagtct ttcccctggt cactacaatg gtcgtccttc tgtaaaggtt
     4381 tctattcaaa aagaacaaat ggttcagcgc gacgccttgg aaggttggaa tgaggcggtt
     4441 tcggattctg tagaggaaaa tacgatgctc tatagagtgg ggcgcatcat attttccaag
     4501 gtgaaattaa aaaagatgga agaacagcgt attaaagacg ttttggatca gggcgtttga
     4561 tattgggcgc tttggcctga agtgctcgaa tagttttttg cacttctgtg cggagtttta
     4621 tgtctttagg atatttcttg gcgtactcgc ctaatttagg aagagcagct tcgtagcgga
     4681 actcacgcaa cacttttaat atgtgctggt gtatccataa cgccttgcca ccgtgggtgt
     4741 tggacgggtc aaagagcgcg gccaataatt ttttagaaga ggtttttaat tttagctgtc
     4801 caatggcgtc gaccgcactt gttcgaataa caagggcctc atcctgaagc cgcgattcga
     4861 tatggggagc gagctcccga gcatttagcg cgactgcgct ttttatagca gcgtcgcgta
     4921 acagccagct cgagtgcgac atgtaagttc ttagtgtctc gagagatttt tttccgttga
     4981 ggcgagctac accgatgagg gaggcccacc tcatagactc cgaggagcta tttgattcta
     5041 aaatttcgtg caatatatcg ctagttttag gcccgaactt ggagtgtagg gctccgatcc
     5101 aggcgctgac ggagcgattt ttttgtacgg tagaagagaa caagctggtt gcagtgtcga
     5161 ggtctttgct gcgtagcgaa ttcagtttat ttggtttcgg aacagacgcg tgggcggaag
     5221 ccaaaaacat taagcttgct cccaaaaaag caccaaaatt gaatttagtc attttcattc
     5281 ggacttcacc ccgagagcat gcgttcgaat tcacttaaca ggtctgtgtc acttttgtcc
     5341 ggcttcgctg cggcaacagg ggcagcgctc ggtttaatgt cggtcgcttg cgctgctggt
     5401 gtcggcggag ccgcagtgtc tgaagcaggt ggagctgttt ttgcagcagc aaccgccgcc
     5461 ggatctacgg cactagcgcc cgctggagta gccaaactct cttcaacttt gtcgactaac
     5521 tgttcaaatt ggtcattggc tttgttctcc gccactgctt gttctgccgc agctataggc
     5581 gaggcattcc cagcagcact ggcagctgga gcgacactcg cggctggagc ggtagcgctg
     5641 gcggctggag cagcactcgc ggctggagcg gtagcgctga cggctggagc ggtagcgctg
     5701 acggctggag cgccagtgtt tggc
//
LOCUS       DAOMXO010000051         5707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7477_length_5707_cov_5.007608, whole genome shotgun sequence.
ACCESSION   DAOMXO010000051 DAOMXO010000000
VERSION     DAOMXO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7477_length_5707_cov_5.007608"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /locus_tag="PLH57_01925"
     CDS             <1..1867
                     /locus_tag="PLH57_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01398.1"
                     /translation="ASKVASNSSSATTTASANFITISKVVRSNLNPDTTIELIGSNGQ
                     LATYCPTADACQCQFDFTESGGVPAQRFQALSYVEVNMARCLYTQVSSNAGYYDVSIF
                     INSANANSNKTRVFMNSISPSLDPSVAANYVETSRYMCRDVIRKSRSATNFYGQNLQD
                     PKAWLLSQAYNFYTTSLGLDYGAHAVAIGESVENTPGFECPPVPNDSTFDASIRYEVY
                     SVDPLSFSDYGGSATNDNTIYPPDDKVGIDPDCPTGTEADCEKFLVNRHDFALAKFQD
                     GTFKQPVCTLHTPANMVNPSLSCTTTGISAPVTLAKTTASSVGKDIIGFAALPNANQE
                     CPNTSVVNLPAGKKWAKLWRFRASLEKRTMESASNSGQIGYLYCTTQNQECSEPTADQ
                     NSACWDADPQGSGTTPGIIGPRPMVPSGAGGTYFGNCSTSGVIGDNWAPGGGYLNAGN
                     ACSSAAVQAVNGGGSCCYDYGATGAGRVAANYTQGGNYCTPGLTGLWDLPAIGGENVW
                     LMGSNSTACIEADTDAAGRLFFNGIIGPDPYRRSSIDIDASSTFDYLYVVTPASVRME
                     DMTDPNSTSARKFKPYRKDRNNNNIHYGISLGSENTATQFERLVKFPLCVLQDSE"
     gene            1879..3780
                     /locus_tag="PLH57_01930"
     CDS             1879..3780
                     /locus_tag="PLH57_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01399.1"
                     /translation="MKTKMNNTHILFAALFAVATLSFGALSGCVNTGGDTSSIVRKNA
                     ASTSGTSSSSAGPTFNIYNATFEAVGPSGASGATGAPATHLNLYLNAGATATLNSVCV
                     VQSGGGADTGKACSCRYSWSETNSSDSTTITRTADAEPDQITSFQMRCALPPAWTNSE
                     ILDNTQVRVSIIPNSAAGSTQSFATNTVLVTKTTVTSQGDFRDIEGRSYKNIYHYVCY
                     DRAQKNQGIFRNLQNVSNGTTTNTVPVANDFVLGAGAFTGQSYYYDFYIRNSELGSPN
                     SSANGFVCPTTNIAGVQSFYPMDTTFALALNSSKDFPVQVNSLTVVPIGTSQSIIGTI
                     GYAAKPQSDGSCPSFADSNGQIRRTFRLRKYSAAYPLRYNADGDPLDQSQQINTLYVM
                     DRPVNKATVDPLKPITRLGPKPCPFSFRTAQFGQRCMSDASLTGWSIDGDQIDGNPKC
                     PVFPPVPNEVQKTDGTVVVRPYQAFVPRYLEDTTFEGCAFQSSTPVDPEIVLSTDSTI
                     FPAPVGPYDFWCAKHYPPADPNPLNRAPGECSLADSAAAVKTDTTYACQLSYNPINAV
                     RKTPQAGCCQVCSGTDCSAVGGGTTPAGRNAAFSPPQDVGNPAQGIKQLSRGTPNVSA
                     SSCFDPSED"
     gene            complement(3802..4266)
                     /locus_tag="PLH57_01935"
     CDS             complement(3802..4266)
                     /locus_tag="PLH57_01935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRK01400.1"
                     /translation="MSTPSKLHRFLNLAVLAMLALSLAFVACTKKAAETQNSQEATNM
                     SAMTPLKIEDSKVGDGTEAKAGNMVTVHYTGWLTDGKKFDSSLDRNNPFSFKLGAGQV
                     IRGWDEGVAGMKIGGKRKLTIPPEMGYGAAGAGGVIPPNATLVFEVELLGVK"
     gene            4345..5697
                     /locus_tag="PLH57_01940"
     CDS             4345..5697
                     /locus_tag="PLH57_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01401.1"
                     /translation="MKVRGIGVTFAGALLLVTLTPGVVLAESCKELVGRLTDNDLSQR
                     RELTNLTRELETLTQKSIDSTDEKPIRGLLRKLEAIAVSPALNETTRVKAVEVLRNTA
                     VSLRSVSGSDLQELLFRFLLVGLHTGARNQVSILERTHEANLDALASLALPPRWASRF
                     VLSRVLPTEDRMALYLHFSAAPQIRWQLFEQAAREPYESEIPLLEYVAIDTGFANLAE
                     ALDDHRRVAAAIKVLDQGSTFGEWTSSDKGLVADLPNAYFFSNFQASITRRLGEVAQS
                     GQYTALRTLFRLAIHPIDDEMTELAQKEIMRLFDSGLKDSHLNEHLPSILEYVEAPLF
                     IAWLVRRNLNEPLLLDLLKKIETIEVLTFKEALERHREFAFSNRPQARKVENQNFSGQ
                     AVFVSYDLRMVPIRSALNELQYFRDLPPGALLDRVARVNASLERARVEREKELVKITP
                     "
BASE COUNT         1399 a         1508 c         1445 g         1355 t
ORIGIN      
        1 ggcttctaaa gtggcctcga atagttcgag tgcgaccaca actgcgagcg cgaattttat
       61 tacaatctca aaagtggtcc ggtcgaactt gaaccccgat actaccattg agctcattgg
      121 ttcgaatggt cagttggcca cttactgccc aacggccgat gcttgccagt gccaatttga
      181 ttttaccgaa tctggtggag ttcctgccca acggtttcag gcactttctt atgtagaagt
      241 gaacatggcc cgttgtctct acactcaagt ttcatcgaac gcgggttact acgatgtttc
      301 gattttcatc aattcggcca acgcgaattc taataaaacg cgagtgttca tgaattcaat
      361 aagtccatca ctggatccga gcgtggcggc aaattacgtt gaaaccagcc gctacatgtg
      421 tcgcgacgtg attcgcaaat cacggtcggc tactaatttt tacggtcaaa atctacaaga
      481 tccaaaagcc tggctcttga gtcaggccta caatttttac acgacgtcac tggggctcga
      541 ttacggcgcc catgcggtgg cgataggaga gagtgtagaa aacacgccgg gttttgagtg
      601 cccgcccgtg ccgaacgatt caacgttcga tgcttcgatt cgttacgaag tttatagcgt
      661 agatccactt tcgttttcag attacggcgg tagcgcaaca aacgacaaca ccatttaccc
      721 gcccgacgac aaagtgggaa tcgaccccga ctgtccgact ggtactgagg cggattgcga
      781 aaagttttta gtaaatcgtc acgatttcgc actcgcgaaa tttcaggacg gcacgttcaa
      841 acaaccagtg tgcacgttgc acacgccggc aaacatggtg aacccgtcgc tctcttgcac
      901 taccaccgga atttctgcgc cggtgacgct tgcgaaaaca acggcctcat cggtgggtaa
      961 agacattatc ggttttgcgg cactgccgaa tgcgaatcaa gaatgtccta acaccagcgt
     1021 ggtcaattta cctgctggca agaaatgggc aaaactctgg cgctttcgtg caagtttaga
     1081 aaaacgcacc atggaatcgg cgagtaattc aggacaaatt ggttatctct actgcacgac
     1141 ccagaatcag gagtgttcgg aaccaacggc ggaccaaaac tcggcttgtt gggatgcaga
     1201 tcctcaagga tcaggaacca caccgggaat cataggacca cggccaatgg taccgtcggg
     1261 cgctggaggc acctatttcg gcaactgcag tacgagtggc gtgattggcg ataattgggc
     1321 acctggaggt ggctacttaa atgctggaaa tgcttgttcc tcagccgcag tgcaggccgt
     1381 aaatggcggg gggtcttgtt gttacgacta cggtgctact ggcgccggaa gagtggcggc
     1441 gaattacacc caaggcggaa attactgtac gcctggcctt acgggattat gggacttgcc
     1501 agcaatcggc ggcgaaaatg tgtggctcat gggtagcaat agcaccgctt gtatcgaggc
     1561 cgacaccgac gcggccgggc gtttgttttt caacggcatt attgggcccg acccgtatcg
     1621 cagatcgtcg atcgacatcg atgccagttc aacgtttgat tatctatacg tagttacacc
     1681 cgctagcgtg cgcatggaag acatgaccga tcccaattcc accagtgcgc gtaaattcaa
     1741 gccgtaccgc aaagatcgca acaacaataa tattcactac ggaatttcat tgggttctga
     1801 aaataccgcc acgcagtttg aacgactggt gaagtttcca ctatgcgtat tacaggacag
     1861 cgaatgagga ggggcgagat gaaaaccaaa atgaataaca cccacatcct cttcgccgcg
     1921 ttattcgcgg tggccacgct ttcgtttggc gcgctttcgg ggtgcgtgaa taccggcggc
     1981 gatacgtctt cgattgtacg aaaaaacgct gcgagtactt caggcacgag cagcagcagc
     2041 gccgggccga cgtttaatat ctacaacgcc actttcgaag cagtcgggcc gagcggtgca
     2101 agtggcgcca ccggtgcacc ggcaactcat ttaaacctct atttaaatgc gggtgccact
     2161 gccaccctta actcagtttg cgtagtgcag tcgggcggag gcgccgacac gggtaaagcc
     2221 tgtagctgcc gctacagctg gtcggaaacc aattcgagtg attccactac tattactcgc
     2281 accgccgacg ccgagcccga ccaaatcacg agttttcaaa tgcgctgcgc gctcccgcca
     2341 gcttggacca atagcgaaat tctcgacaac actcaggtgc gcgtgagtat tattccaaat
     2401 tcagcggctg gcagtactca aagttttgcg actaacacgg tacttgtcac caaaactacc
     2461 gtgacgtcgc agggtgactt tcgcgacatc gaagggcgct cttacaagaa catttatcac
     2521 tacgtttgtt acgatcgtgc gcaaaagaac cagggcattt ttcgcaatct tcaaaacgtg
     2581 agcaatggca ccactactaa cacggtaccg gtggccaacg acttcgtatt gggcgcgggg
     2641 gcgttcactg gccaaagcta ctactacgat ttttatattc gaaactccga actgggcagt
     2701 ccgaactctt ctgccaatgg ttttgtgtgc ccgaccacca acatagctgg agttcaaagt
     2761 ttttacccga tggacaccac ctttgcgctg gcgctcaatt cgagcaaaga ttttcccgtg
     2821 caagtaaact cgctcacggt tgttccgatc ggaacaagtc agagtattat tggaaccatc
     2881 ggctatgccg ccaaaccgca gtctgatggc tcgtgtccaa gtttcgccga tagcaacggc
     2941 cagattcgcc gcacgtttcg gctgcgaaaa tattcagcag cttatccttt gcgctacaac
     3001 gccgacggcg acccgctcga tcagtcacaa caaatcaaca cgctctacgt aatggaccgt
     3061 ccggtcaaca aagcaaccgt cgacccgtta aaaccaatca cgcgattggg gcccaaacca
     3121 tgtccgttta gttttcgaac tgcgcagttt ggccagcgct gcatgagcga cgcctcgctc
     3181 acagggtgga gcatcgatgg cgatcagatc gacggcaatc caaagtgccc ggtgtttccg
     3241 ccggtgccaa atgaagttca gaaaacagac ggcaccgttg tggtgcgtcc ttaccaggcg
     3301 ttcgtgccac gctacttaga agacacaacg ttcgaggggt gcgcatttca gtcttccacg
     3361 ccagtcgatc cagaaatcgt gctttcgact gattcgacga tcttccctgc gccggttggc
     3421 ccttacgact tttggtgtgc caaacattat ccaccagccg acccgaaccc actgaaccgt
     3481 gcgcccgggg agtgttcgct agcagattca gcggctgccg tgaaaaccga tacaacgtac
     3541 gcgtgccaac taagctacaa cccaatcaat gcggttcgaa aaacacctca agctggatgt
     3601 tgccaggtgt gttcgggaac cgattgttct gccgtgggtg gcggtacaac tcctgctggc
     3661 cgaaacgcgg cgttctcgcc cccgcaagac gtgggcaacc ccgcgcaagg catcaaacaa
     3721 ctctcgcgtg gcacacccaa cgtgagtgcg agttcttgtt ttgatccaag cgaagattaa
     3781 ttcaaatttt tgtgtgaagt cttatttcac gcccaagagt tcgacttcaa acacgagcgt
     3841 cgcgttaggc ggaatcacgc caccggcacc ggctgcgccg tagcccattt ctggtggaat
     3901 ggtgagctta cgtttgccgc cgatcttcat gccggccact ccctcatccc aaccgcgaat
     3961 cacttgaccc gcgccaagtt tgaacgagaa cggattatta cgatcgagcg aagagtcgaa
     4021 ctttttgccg tcggttaacc agcccgtata atgcacggta accatgttgc cggccttcgc
     4081 ctcggtgccg tcgcctacct tggaatcttc aatcttaagg ggagtcattg cgctcatgtt
     4141 agtagcctcc tgggagtttt gagtttcggc cgcctttttg gtgcaggcca cgaatgccag
     4201 gcttagcgca agcatggcga gaacggcaag gttcaaaaaa cgatgtaatt tcgacggtgt
     4261 actcattgcg tttccctttc ctacgtttgt gaaaagtata ccgatcattg atttttattg
     4321 tcaacattag tacaagccac agccatgaag gtaaggggaa ttggggttac tttcgccggg
     4381 gcacttcttt tggttacgct cacaccgggg gtggtccttg cggaaagctg taaagaacta
     4441 gtagggcgat taaccgataa cgatttatcg cagcgtcgcg aacttaccaa cttaactcgc
     4501 gaactagaaa cgctcacaca aaaatccata gactctaccg acgaaaaacc aatccgaggt
     4561 ttacttcgaa agctagaagc cattgcagtt tcaccagcgt tgaacgaaac cacgcgggtg
     4621 aaggccgtag aagtcttgcg caacacggct gtttcgttac gctcagtaag tggttccgat
     4681 cttcaagagc tactttttcg ctttctgttg gtgggacttc acacaggagc acgaaatcag
     4741 gtatcgattc ttgaacgcac tcacgaggcc aatttagacg cgctcgcttc actagcatta
     4801 ccgccacgtt gggcctcgcg ttttgtactc tctcgcgtgc ttccgacaga agatcgaatg
     4861 gcgttgtatt tacatttttc tgctgctccc caaattcgtt ggcagctctt cgaacaagcg
     4921 gctcgggaac cctacgaaag cgaaattcct ctgcttgagt acgttgccat tgacacgggt
     4981 tttgcgaatc ttgccgaagc actcgacgat catcgacggg tagccgcggc gattaaagta
     5041 ttggaccaag gatcgacgtt tggagagtgg acgtcgagtg ataaaggttt ggtcgcagat
     5101 ctgccgaacg cgtacttttt ttcgaatttt caagcttcga taacccgccg cctaggggag
     5161 gtcgcccaat cggggcagta caccgccctg cggacgttgt ttcgacttgc gatacacccc
     5221 attgatgacg agatgactga actagcccaa aaagaaatca tgcgcttgtt cgacagcggc
     5281 cttaaagatt cgcacctcaa cgaacacttg ccatcaattt tggaatacgt cgaagcgccg
     5341 ctttttattg cttggctcgt gcgccgtaac ctaaatgaac cgctgctctt agatttacta
     5401 aagaaaatcg aaacgattga agtgctcact tttaaagaag cactagagcg acatcgagag
     5461 tttgcgttta gtaatcgacc tcaggctcga aaagttgaga atcagaactt ttcagggcag
     5521 gcggttttcg taagttacga tcttcgaatg gtaccgattc gaagtgcttt aaacgagttg
     5581 cagtattttc gtgatttgcc gcccggtgcg cttcttgatc gcgtggcacg agtcaatgcc
     5641 tctcttgagc gggctcgagt cgagcgcgaa aaagagcttg taaaaatcac gccctaattt
     5701 ttttaag
//
LOCUS       DAOMXO010000052         5686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7502_length_5686_cov_4.106908, whole genome shotgun sequence.
ACCESSION   DAOMXO010000052 DAOMXO010000000
VERSION     DAOMXO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7502_length_5686_cov_4.106908"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PLH57_01945"
     CDS             <1..1183
                     /locus_tag="PLH57_01945"
                     /inference="COORDINATES: protein motif:HMM:NF025805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pre-toxin TG domain-containing protein"
                     /protein_id="HRK01402.1"
                     /translation="KTAYEKSAQSIQLFSADSIRLKTSLGNLNARGDSSAKAIANLCE
                     NQNKIAEDLKLSQAEEIADFEEKLEKAISSAPQGKDGLLAGFDTQKFKTKATTAEGSE
                     IRYLWNRQAVIKSWSNDSLFKFNKTLLTVSEDLILAYDSIAAQAKIATEQQIQARATI
                     HSMQNYAAGIFSFSLGFVPGVGTAKDIAEIATGVDIITGFKLSNDERALVALGMLLPV
                     AGSAAKTAVKAVAPDLLTELAKKSGSIWDKVVTRAVELNKTGVWVKRSADELNAEWAQ
                     FGAEPAWMAGSYVYEVVLKQPVRLWRVTERTEDAAGKWYMKHHPTNFSKSELADSLAL
                     PGGVDGIKNIVEIEIPAGTQLLGGIAGPNKWGKGGGKQWYLNFNGDIPEDWVKGVSKW
                     E"
     gene            1174..1560
                     /locus_tag="PLH57_01950"
     CDS             1174..1560
                     /locus_tag="PLH57_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01403.1"
                     /translation="MGINIFYAKENRVLKKNPDTETEIELIVFNDRIKQVLPTDHGLI
                     VILGGKGSTKPGNIFLINASSGTIVWQSEPCPGLSIDGKDKKEIYASARVEDGKLIAR
                     AYDGLEHEVDWQTGKHLKVTGLRRFG"
     gene            1810..2184
                     /locus_tag="PLH57_01955"
     CDS             1810..2184
                     /locus_tag="PLH57_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01404.1"
                     /translation="MGIIQFYGSDNKVLKKTDESIVELIILSDRVSQVLETDRGLIVI
                     LGGLGRTKPGNMYLIDVGSGSIIWQAEAAPGISYDQKERKCVYGKARVEEDKLVVWAE
                     DGLEHEVDWHTGKHLKVLRLVK"
     gene            complement(2455..2970)
                     /locus_tag="PLH57_01960"
     CDS             complement(2455..2970)
                     /locus_tag="PLH57_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRK01405.1"
                     /translation="MDWESKILSVTKSYNCRMRETKSTKAGTWRQVPMSNKLIEILRT
                     QYKATGQEQHVFERAWQWERGLQAKVLRTFCYIHGLPSIKFHTLRACFATQMLRQGVE
                     AAKVMKICGWKELKTMQHYVRLAGIKVCGATEALEIFDRSKERSNPLQNHGASLELDT
                     SQAHDFATTQV"
     gene            3137..3430
                     /locus_tag="PLH57_01965"
     CDS             3137..3430
                     /locus_tag="PLH57_01965"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRK01406.1"
                     /translation="MTYTYKVIYNMTMGEMFRFGGIIIRVWSNDHDPPHVEAFWPSMK
                     RPEAHAKFALDTLECIACDGFSARDVRQIQEELRKRHEKLWEGWRQIHGQEED"
     gene            3411..3659
                     /locus_tag="PLH57_01970"
     CDS             3411..3659
                     /locus_tag="PLH57_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017297539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HRK01407.1"
                     /translation="MAKKKINYGKKDLLGPDEFDPKTAKERITIWLDEEILDIFRERA
                     KAEGTKYQSLVNQALREAARKPSLVERIERLEKKLSNG"
     gene            complement(4058..4717)
                     /locus_tag="PLH57_01975"
     CDS             complement(4058..4717)
                     /locus_tag="PLH57_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01408.1"
                     /translation="MKKSLWQAREVRDKALEEAYEYFDQKIRLLPKLPTGKVNPTARG
                     LEDNDVDAFRHAYVSGVFTQVFGENTADVFGRLNELDPTSLYSNSQNPGSENMDLWNN
                     SIGRKYGKKTNSRKVLLRKIHEALKKGELITDPDDTRKYRGAKGDPGKSSKPVVAIGK
                     SKSGRNERFYDTSKQIEFSREEFIAKIEEGAYPGYTVKNIRGTPTPVSNPDDRRSNNH
                     S"
     gene            complement(4714..5031)
                     /locus_tag="PLH57_01980"
     CDS             complement(4714..5031)
                     /locus_tag="PLH57_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01409.1"
                     /translation="MKWLSRALVLIVSFLSANLIAEVGEPKPYYVKLPIGFAAALAWD
                     FPLWISTFKVATKWRWPLALAILLTGIATIIAMCFYNLIIASGVAAIHLYLIVNLITA
                     KNT"
     gene            complement(5556..5645)
                     /locus_tag="PLH57_01985"
     tRNA            complement(5556..5645)
                     /locus_tag="PLH57_01985"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5609..5611),aa:Ser,seq:cga)
BASE COUNT         1520 a         1271 c         1379 g         1516 t
ORIGIN      
        1 aaaaaccgca tacgaaaaat cagcccagtc gattcaactt tttagtgccg attcgattcg
       61 tttaaaaaca agtcttggca atttaaacgc ccgaggtgac tcgagcgcaa aagccattgc
      121 taatctctgc gaaaatcaaa acaagattgc agaagatcta aaactttccc aagccgaaga
      181 aattgctgat ttcgaagaga agctggaaaa agctattagc tcagctccac aggggaaaga
      241 tggtttgctt gctggttttg atactcagaa gttcaaaaca aaggcaacca ctgcagaggg
      301 ttctgaaata cgttatcttt ggaaccggca ggccgttatt aaatcgtggt ctaacgactc
      361 gttatttaaa ttcaacaaaa cattactcac ggttagcgaa gatttaattt tagcgtacga
      421 ctcaattgcc gcgcaggcga aaattgcaac tgaacaacaa attcaagcgc gcgctacaat
      481 tcattccatg caaaactacg ctgcgggaat attttctttt agcttaggtt ttgttccagg
      541 tgtgggtacg gctaaagaca tcgccgaaat tgctacaggt gttgatatta tcactggatt
      601 taaactttca aacgacgagc gggcgcttgt tgcgttaggc atgttgcttc ccgttgcggg
      661 aagtgcagca aaaactgcag taaaagcggt tgccccagac cttctgactg agttagcaaa
      721 aaagagcggt tcaatttggg ataaagtagt cactcgcgca gtcgagctaa ataaaactgg
      781 agtttgggtt aaacgcagcg cagatgagct caatgccgag tgggcacaat tcggcgcaga
      841 gcccgcgtgg atggccggaa gctatgttta cgaggtagta ttaaaacaac cggttaggtt
      901 gtggcgggtt accgaacgca cggaagacgc cgctggaaaa tggtatatga aacatcaccc
      961 cactaatttt tcaaagtccg aactagcaga tagtttggca cttcctggtg gcgtcgacgg
     1021 aataaagaat attgtagaaa tcgaaattcc tgcgggaact cagcttttag gtggtattgc
     1081 tggaccaaat aaatggggca aaggaggcgg gaagcagtgg tatttgaatt ttaatggaga
     1141 cattcctgag gactgggtga agggagtatc aaaatgggaa taaatatatt ttacgcaaaa
     1201 gaaaataggg ttcttaaaaa aaatcccgat actgaaaccg aaattgaatt aatagttttc
     1261 aacgatcgaa tcaagcaggt tcttccaaca gatcacggtt tgatagtaat tctcggtggc
     1321 aaaggaagta caaaacctgg aaatatcttt ttaataaatg catcgagtgg aacaatagtt
     1381 tggcaatctg aaccatgccc cggactaagc attgacggta aagataaaaa agaaatttat
     1441 gccagcgccc gggtagaaga tggaaagtta attgctcgtg cttacgacgg cctcgaacac
     1501 gaagtcgact ggcaaacagg aaaacatcta aaagtaacgg gtttgcggcg ttttggataa
     1561 agagtttaat atctgaaaca ctcatccaac taatttcaca aaggatgagc tggtcgatag
     1621 tctagcttta ccgaatggca tcgcaggaac aaaatatatc gctgaagttg aaattccggc
     1681 tagtacgcgc ctgtttggag gaatcgctgg tccgaataag tggggcaagg gcggtggaaa
     1741 acagtggcag gttcaattgg acggagaagg agatttacct gttgagtgga ttaaaaagga
     1801 acggatagaa tgggaataat tcaattttac ggatctgata ataaagttct taaaaaaact
     1861 gacgagtcaa tcgttgaact aattattctt tccgatcgag tttcccaagt tttagaaacc
     1921 gatcgtggct taattgtaat tttgggcgga ctgggccgaa ctaaacctgg gaatatgtat
     1981 ttgatcgacg ttggttccgg atcgattata tggcaagccg aagccgcccc gggaataagt
     2041 tacgatcaaa aagagcgaaa atgtgtatac ggtaaagccc gagtagaaga agacaagcta
     2101 gttgtttggg cagaggacgg cctcgaacac gaagtcgatt ggcacactgg aaagcattta
     2161 aaagttttga gattggttaa ataaaacagc aactggcgct acaatgttaa gggatcagga
     2221 cattgatggg accggatggc ggagagggtg agattcgcct atctgtcttc gcgctgttcg
     2281 cgcttaagca gacaggcccg cctcactcgc tgcccactgg cgtgggcacc cactcgctcc
     2341 ggcgcttctc atcccaccca aaggtgaaaa attacttcgt aatttttata ttagataccg
     2401 ccctaccgcc tggcggcgga tatggcggat tcggcgtttg gcgctgattc cgtattaaac
     2461 ttgagtggtt gcaaaatcgt gtgcctggga tgtgtccaat tctagtgatg ccccgtgatt
     2521 ttggagcggg ttcgatcttt ctttcgagcg atcgaaaatc tccaaagcct cggttgcgcc
     2581 acagacctta atgccagcaa gtcttacgta atgttgcatc gttttgagtt ccttccaccc
     2641 acaaatcttc atcaccttgg ccgcctcgac accctgccgt agcatctggg tagcaaagca
     2701 agctctgagc gtatggaatt tgatggatgg aagaccatga atataacaaa aggtccgaag
     2761 cactttcgct tgaaggcctc tttcccattg ccacgctctt tcgaagacat gctgctcctg
     2821 gccggtggct ttatactggg tgcgcagaat ctctatgagc ttgttactca tgggaacctg
     2881 acgccaagtt ccagccttcg tcgatttcgt ctctcgcatc cggcagttgt aggatttcgt
     2941 gaccgagaga attttgctct cccaatccac gtccttccac cagcggttaa ccacgtcaaa
     3001 ttttcaaaga acaatgcagt catctttacc ggagaatctc ctggaatcgc aatggggatg
     3061 cttttggaag tctcatccat tgtgaatccc ttattttgtt gattaaaatg ctcttatttt
     3121 caggtggatg cgcagcttga cttatacata taaagtcata tataatatga ccatgggcga
     3181 aatgtttcgc ttcggcggca tcatcatccg agtttggtcc aacgatcacg acccacccca
     3241 tgtcgaagcg ttctggccaa gtatgaaaag accagaggct cacgccaagt ttgcgcttga
     3301 tacgctagaa tgcatcgctt gcgatgggtt ttcggcgaga gacgttcgac aaatccaaga
     3361 ggagcttcgt aaacgtcacg aaaaactttg ggaaggatgg aggcaaatcc atggccaaga
     3421 agaagattaa ttacggaaaa aaagaccttt taggtccaga tgaatttgat cctaaaaccg
     3481 ccaaagagcg aatcaccatt tggctcgatg aagagatcct agacatcttc cgcgagcgcg
     3541 caaaggccga aggcacgaag tatcaatcgc tagtgaacca agctctacgt gaagccgcac
     3601 gcaaaccatc gcttgtggaa cgcatcgaac ggttagagaa aaagcttagc aacggataaa
     3661 cctcggtaaa aatagatccg gctgtgcatg attccatttt cttgctcgga actctgggac
     3721 tgattttacc gtttgtattt ttttcttctg atccctgtat ttctttattc tacttcgatt
     3781 ctatttcctg cctttgactt tcgggagcgg aggcaagtct ttgtcttgat tcagaatcgg
     3841 aagcagcagt cccgctcacc tcaagtacgg ctttcctgct cacctcctgg gcagttcgcg
     3901 ggccacctcg cgatgattcg gtgagtatgg attttaagtc cacccaatcg cgaagtaatc
     3961 ctggcggttg atccggcgtg aacacccacg cggtcgctgg atcgcccaga acttcattga
     4021 gaatggcttg actattgagt gctaaggcgc agacctttca agaatggtta ttcgatcttc
     4081 ggtcatctgg atttgaaaca ggcgttggcg taccgcgaat atttttcacg gtatatcctg
     4141 gataagcgcc ttcttcgatt tttgcgataa actcttcgcg gctaaattct atctgcttag
     4201 atgtatcata gaagcgttca ttgcgtccgg atttactttt accgatagct accacgggct
     4261 ttgagctttt tccgggatca cctttagctc ctctgtattt tcgagtgtca tcgggatcgg
     4321 tgatgagttc tccttttttc aaagcctcat ggattttcct tagaagcact ttgcggctgt
     4381 tggttttctt tccatatttg cgtccgatgg agttgttcca caaatccatg ttttccgacc
     4441 cgggattttg cgaattcgag taaagactcg ttggatcaag ctcgttgagt cgtccgaaaa
     4501 catccgccgt attttctcca aagacttgtg taaacactcc gctgacatat gcgtgtcgaa
     4561 aggcatcgac atcgttgtct tcaaggcccc tagcagtcgg attcactttt ccggttggta
     4621 gcttcggcaa aagtcgaatt ttttgatcga agtactcgta agcttcttcc aatgccttgt
     4681 ctctgacttc cctggcttgc cataacgact tcttcatgtg tttttcgcgg ttattaaatt
     4741 tacgattaag tagagatgaa ttgccgcgac gccactggca atgatcaagt tatagaaaca
     4801 catggctatg atagtcgcga tgccagtcaa aagaattgca agcgcaagcg gccaacgcca
     4861 tttcgtggcc accttgaaag tacttatcca aagaggaaag tcccacgcta gcgcggcagc
     4921 gaacccgatc ggcaacttga catagtaagg tttgggttca ccgacttcag cgataaggtt
     4981 tgcgcttaga aaacttacga tgagaaccag cgctcttgag agccacttca ttgtttaaaa
     5041 gtatattcgg gttagcgctg ggtcaatcca agtcgcggaa tttccacagt ttagttcttg
     5101 attctagcgc ggtatgacgt gtgcccaccg cgtgtccaaa gtgtgcccaa ccggtgtgac
     5161 aatcggtgat gatctgagac ggccctcacc gggtagcaag ggttcgttaa catagctgct
     5221 taaaacccta aatcaggggt tcgcattctg gaaatgtttc cggttatacg tgtaaacagt
     5281 ttttaggggt gcagtatagt ttctaggtgg ttactgtgtt tgcgtttttt gctcttttcg
     5341 ccgttcgagg atgatcccgg cgattttgaa tggcggagag ggtgggattc gcctatctgt
     5401 cttcgcgctg ttcgcgccta agcagacagg cccgcctcac tcgctgccca ctggcgtggg
     5461 cacccgctcg ctccggcgct tctcatccca cccaagggtg aaaaattact tcgtaatttt
     5521 tatattagat accgccctac cgcctgcggc ggatatggcg gagagggtgg gattcgaacc
     5581 cacgttaccc gttaaggcaa acacgctttc gaggcgtgcg gtttcaacca ctcacccacc
     5641 tctccgttta ctgactattc aactcttaaa gaacaattca aacttc
//
LOCUS       DAOMXO010000053         5683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7513_length_5683_cov_4.776119, whole genome shotgun sequence.
ACCESSION   DAOMXO010000053 DAOMXO010000000
VERSION     DAOMXO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7513_length_5683_cov_4.776119"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1589
                     /gene="hutH"
                     /locus_tag="PLH57_01990"
     CDS             72..1589
                     /gene="hutH"
                     /locus_tag="PLH57_01990"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRK01410.1"
                     /translation="MREIQLDGQSLTLEVLAEVAYASHDNVRVSLSKKARDRMDRSRK
                     VIQSMLRGDDAIYGVNTGFGLLSQVRVDKNKLDQLQINLLRSHAAGVGQPLSIPEVRA
                     AILLRANTLAQGSSGVSTQLVEALLNLLNRGVHPWIPEQGSVGACGDLAPLAHLSLVL
                     IGEGEAFFRGKLISGAAALKAAKLKPYKLQPKEGLSLINGTQIMTAIGALTFLRAEAL
                     AQLSDVAGAMTIEALCGSKVPFDEDLHKTRPHRGQMEVAKNMLRLLEDSEIMASHANC
                     TRVQDPYSLRCIPQVHGVARDTLRSVRKVLEVEINSVTDNPLVFVSGKKSKIVNGGNF
                     HGEYVGNAMDQLAIVVSELANISEQRIEKLINPNFSGLPPFLVDGEGLNSGLMIVQVA
                     AASLVSENKILSHPATVDSIPTSADKEDHVSMGTIAARKARSVVENSWNVIAMEFLAN
                     SQALEFRKPLAPAKKLKKTLAAIRTRVPVIKADRAFHKDVEAIADLMRTGHLSTR"
     gene            1592..3250
                     /gene="hutU"
                     /locus_tag="PLH57_01995"
     CDS             1592..3250
                     /gene="hutU"
                     /locus_tag="PLH57_01995"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010171036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRK01411.1"
                     /translation="MGRILRANHGVELECKGWVQEAALRMLMNNLDPDVAEKPEDLVV
                     YGGIGKAARNWPSFNKIVSELKRLGNNETLLIQSGKPVAVFPTHTDAPRVILSNSMLV
                     PRWATWDHFRELDAKGLMMYGQMTAGSWIYIGTQGIVQGTYETFASAGRKHWNSDLAG
                     KFVVSGGLGGMGGAQPLAAAMAGAVFLGVDLQEDRIKRRLDTRYLDVHAKSLDEALNL
                     ARDACAKRKALSIGLTGNIIDVLKKMLTQKVKIDLLTDQTSAHDELNGYFPEGLTLSE
                     ALSLRKQDPETYRRRSLESMITHVKLMQEFQKLGTHVFDYGNNLRGQAQAAGHKDAFT
                     FPGFVPAYIRPLFCEGAGPFRWVALSGDPEDIRVTDEALLNLFPENKSMARWLSMAEE
                     RIQFQGLPARICWLKYGEREKAGVLFNQLVAQKKVKAPIVIGRDHLDCGSVASPNRET
                     ESMKDGSDAVADWPILNAMINSINGASWVSVHHGGGVGMGYSIHAGMVIVADGTQEAE
                     RRIVRVLNSDPGMGIVRHCDAGYEEANRAYKPADKNKIRIPFLE"
     gene            3255..4493
                     /gene="hutI"
                     /locus_tag="PLH57_02000"
     CDS             3255..4493
                     /gene="hutI"
                     /locus_tag="PLH57_02000"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRK01412.1"
                     /translation="MAKKIFFHSLNELITNAGVYKKAGVGVQERDLGIIENGALLWDS
                     AKGILWLGKTKDAPKALVRGSKQIDCRGHFLSPALTDCHTHLVFAGSRHEEFASRVAG
                     LSYQEISAKGGGIRSTVMATRKASEKELFNVALERIAVMKSHGVGVLEIKSGYGLDWV
                     TERKQLRVIQQLRKKSDLRIFSTFLGAHAFPPEAITEKQRNAYVDTVVNEMLPAVRRE
                     KLADACDVFFDDGYFTEDQARRILQAAIFQKLHIKLHADELANTGGAKLAAEMSALSA
                     DHLLKADVDGISRMAKNGVVAVLLPGTAFYLGLKYPDVAMMRKQGVCFALATDYNPGS
                     SPTTNLPFMMSLGCLQMGMTLPEAFAAATYGGANALGLHDSEGALLIGKKPKIALFNQ
                     PSYKALISQFAGVGSCRLLL"
     gene            complement(4490..5170)
                     /locus_tag="PLH57_02005"
     CDS             complement(4490..5170)
                     /locus_tag="PLH57_02005"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRK01413.1"
                     /translation="MTLPIFIHASNRIRMAFLCGIAFFLGYTIPNHFHLFEPNLLPLT
                     SWDTAVPFMPWTVFIYTSEYILFVSAYFLFSNEINRNKYIWAYFGVLFTGMLFFVFWP
                     TTYPRGEFPIPPDTHWFTAKVFLTLRTIDNPSNCFPSMHVACCYLTAFAYLPKNESRL
                     KFWIYFIWSTLVGLSTLPTKQHYVVDVVGGILLAVAGYYVFFKKVQYLPAAEYFRRLS
                     FVRRHNQS"
     gene            complement(5173..>5683)
                     /locus_tag="PLH57_02010"
     CDS             complement(5173..>5683)
                     /locus_tag="PLH57_02010"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRK01414.1"
                     /translation="GVTLSVARHFRLKNPISLENPKSADQRQVPNVSFVRFHDQQAVS
                     LEAFKGKVVLINFWASWCSACLVEMPSINRLYETLKNEGFEVIALNVDENPNDVVPNL
                     AKKLELKFPVYLEPKNTLTDFFSVVAIPYSVVLGKDQRVLWAESGERDWSAPAIVEEL
                     RKALKTDSK"
BASE COUNT         1595 a         1254 c         1442 g         1392 t
ORIGIN      
        1 gtcggcgata tttggaagcg actcatacga aaaaatcata gcgaaaatgc ggaaagaagt
       61 gactaaataa aatgcgtgaa attcaactcg acggccagtc tttaacactc gaggtactag
      121 cggaagtggc ctacgcttcg cacgataatg tgcgagtatc actttccaaa aaggcgcgcg
      181 atcgcatgga ccgttctcgc aaggttatcc agtcaatgtt gcgtggcgac gacgctatct
      241 acggagtgaa cactggtttc ggattattga gtcaagtacg tgtcgataaa aataaacttg
      301 atcaactgca aattaatcta ctgagatcac acgcagccgg agtcggacaa ccgttgagta
      361 tacccgaagt tcgtgctgcg attttattgc gagcgaatac cttagctcaa gggagttccg
      421 gagtatctac gcagttggta gaagcgcttt tgaatttact caatcgtggg gttcacccct
      481 ggattccgga acagggcagc gtgggtgcct gtggagatct agcgccctta gctcatttgt
      541 cgttggtatt gattggcgaa ggggaagcct tcttccgcgg aaagctgatc agtggagctg
      601 cggctctgaa ggcagcgaaa ttaaaaccct ataaacttca acctaaagaa ggtctttcgt
      661 taattaacgg gacgcaaatt atgacggcca ttggtgcact cacttttttg agggccgaag
      721 cactcgccca actaagcgat gtagccggtg ccatgactat cgaagcgctt tgcggatcaa
      781 aagtaccgtt cgacgaggat ttacataaaa cgcggccgca ccgaggtcag atggaagttg
      841 cgaaaaacat gttacgtttg ctcgaagatt ccgagatcat ggcgagtcac gcaaactgta
      901 cgcgagtaca ggacccctat agcttacgat gtattccaca ggtgcacgga gtagcgcgag
      961 acacccttcg ttcggtacga aaagtactag aagtcgaaat taacagtgta accgataatc
     1021 cactcgtatt cgtttccgga aaaaaatcga aaatagttaa tggtggcaac tttcacgggg
     1081 aatatgtggg aaatgccatg gaccaacttg ccattgtggt gtcggagttg gccaacatct
     1141 ctgagcagcg cattgaaaaa cttatcaatc cgaatttttc aggattgcct ccatttttag
     1201 tggacggcga aggtttgaac tccgggttaa tgatcgttca agtagccgcg gcttcgttgg
     1261 ttagtgagaa taaaattctt tcacatcctg ccacggtaga ttcaattcca acttctgctg
     1321 ataaggaaga tcacgtaagt atgggcacaa tcgccgctcg caaggcccga tccgttgtag
     1381 aaaattcttg gaatgtgatt gcgatggaat ttttagcgaa ttcccaggcg ttggagtttc
     1441 gaaaacccct cgccccagca aaaaaattaa aaaaaacatt ggccgcaata cgcacgcggg
     1501 ttccggtaat taaagcggat cgcgcattcc ataaagatgt cgaggctatt gctgatctaa
     1561 tgcgaaccgg ccatttgtcg acgaggtaac tatgggtcga attttgcggg caaatcacgg
     1621 agtcgaacta gaatgtaagg gttgggtaca agaagcggct cttcgcatgc ttatgaacaa
     1681 cctcgacccc gatgtggcag aaaaacccga agatcttgtt gtttatggtg ggattggaaa
     1741 ggccgcgcgt aactggccaa gcttcaataa aatagtttcc gagctcaaac gtctcggcaa
     1801 caatgaaacg ctgttgattc aaagtggcaa accggtagca gtgtttccga ctcataccga
     1861 tgcgccacga gtaattttaa gtaattcaat gcttgtaccg cggtgggcga cttgggatca
     1921 ctttcgtgaa ctagatgcca aaggcctaat gatgtacgga caaatgaccg cgggtagttg
     1981 gatttacatt ggtacccagg gaattgttca aggcacctat gagactttcg cttctgccgg
     2041 ccgcaaacat tggaatagtg atctcgcggg aaaattcgtt gtatccgggg gcctcggagg
     2101 catgggcggc gcgcagccat tagcagctgc catggcgggc gccgtatttc tgggggtgga
     2161 tttacaggaa gatcgaatta aacgacgact agatactcgg tatctcgatg ttcacgccaa
     2221 aagcctcgac gaggcactta atctggcccg cgatgcctgc gcgaaaagaa aagcgctctc
     2281 gattggactt accggaaaca ttattgatgt cctaaaaaaa atgctcacgc aaaaggtaaa
     2341 aatagatctc ttaaccgatc aaacgtcagc ccacgacgaa ctaaacggtt attttccaga
     2401 agggcttacg ctctcggagg ccctaagctt aaggaagcaa gatcctgaaa cctaccggcg
     2461 tcgtagttta gaatccatga taactcacgt gaagctgatg caggagtttc aaaaacttgg
     2521 aactcacgta ttcgactatg gcaacaattt acgcggacag gcccaagctg caggacataa
     2581 agacgcattc acctttcccg ggttcgtgcc cgcttacatt cgcccgcttt tttgtgaagg
     2641 cgccggtcca tttcgttggg tagcgttgtc tggtgatccc gaagatattc gagttaccga
     2701 cgaagccctt ttgaatttat ttccagaaaa taagtccatg gcgcgctggc tttcgatggc
     2761 agaggagcgc attcagtttc aagggttacc ggcgagaatt tgttggctca agtacggtga
     2821 gcgtgagaag gctggcgtgt tattcaatca attggtcgca caaaagaaag tcaaagcacc
     2881 gatcgtaatt gggcgagacc atttagattg tggatcggtg gcttcaccaa accgtgaaac
     2941 agaatctatg aaagacggaa gtgacgccgt ggcagactgg ccgatattaa atgcgatgat
     3001 caattcaata aacggcgctt cttgggtgag cgtgcatcat ggcggtggcg tcggaatggg
     3061 atattcgatt catgcgggaa tggtgatcgt agccgacgga actcaagaag ccgaaaggcg
     3121 tatcgtgcgg gtcttaaatt ccgatccggg aatgggaatt gtacggcact gcgacgccgg
     3181 ttacgaagaa gccaatcgag cctataaacc agcagataaa aacaaaattc gaatcccctt
     3241 tttggagtaa atacttggca aaaaagattt tctttcactc actaaatgaa cttattacta
     3301 atgccggcgt ttataaaaaa gcaggtgtag gggttcagga gcgagatctc ggaataatcg
     3361 aaaacggcgc gctcctgtgg gattccgcca aaggcatcct ctggcttgga aaaaccaaag
     3421 acgccccgaa ggctctcgtg agaggcagca aacaaataga ctgccgcgga cattttcttt
     3481 cccctgccct gacggactgt catacccact tggtttttgc gggttctcga cacgaagagt
     3541 ttgccagtag agtggcgggc ctcagctatc aagagatatc cgccaaaggt ggcggcattc
     3601 gtagtacggt aatggccact cgaaaagcga gtgaaaagga actttttaac gtagccctcg
     3661 agcgtatcgc ggtcatgaaa tctcacgggg ttggggtttt agaaattaaa tctggatacg
     3721 gtctcgactg ggtaaccgaa cgtaaacagt tgcgagtcat acaacaatta agaaaaaaat
     3781 cagatcttcg gatattttct acctttcttg gtgcccatgc atttccaccc gaagcaatta
     3841 ccgaaaaaca acgtaacgca tatgttgata cggtagtgaa cgaaatgctc cccgcggttc
     3901 gtagagaaaa actagccgat gcgtgtgacg tatttttcga tgatggatat tttaccgaag
     3961 accaggctcg aagaattttg caggcggcta tttttcagaa gctgcacatt aagctacacg
     4021 ccgacgaatt agccaataca ggtggtgcga aattggcagc cgagatgtca gcactttcgg
     4081 ctgatcattt gttaaaagca gacgtcgatg gcatcagccg catggccaaa aatggggtgg
     4141 tagcagtgtt gttgccgggg accgcgtttt atcttgggtt gaagtatcct gacgtagcaa
     4201 tgatgcgaaa acagggagta tgttttgcgc tcgccacgga ttacaacccc ggaagttcgc
     4261 ccacgacaaa cctgccattt atgatgtcgc tcggttgctt gcagatggga atgactttgc
     4321 ccgaggcatt tgctgcggct acctatggtg gcgccaatgc tttaggactt cacgacagtg
     4381 agggagccct gctaatcggg aaaaaaccta aaatcgctct ttttaatcag ccaagttaca
     4441 aggctttgat ttcccagttt gccggcgtgg gctcttgccg tctgttgctt taactctgat
     4501 tgtggcggcg aacaaaactt aatcgtcgaa aatattctgc tgccggaaga tattgcactt
     4561 tcttaaaaaa gacataatag cccgctactg cgagaagtat tccgcctacg acatcaacaa
     4621 catagtgttg cttggttggg agcgttgaaa ggccgaccag agttgaccaa ataaaataga
     4681 tccaaaattt taaccgcgac tcatttttag gcagatacgc aaatgcggtc aagtaacaac
     4741 acgccacgtg catcgacgga aaacagttgc ttggattatc gattgtacgc aacgttaaaa
     4801 aaacctttgc tgtgaaccag tgtgtatcgg gtggaatagg aaattcaccg cgcggataag
     4861 tggtcggcca gaaaacaaaa aagagcattc ccgtaaagag cacgccgaaa taggcccaaa
     4921 tgtatttgtt tcgattgatt tcatttgaga acaagaaata ggcgcttacg aaaagaatat
     4981 attcactggt gtaaataaaa acggtccacg gcatgaatgg aacggctgta tcccacgaag
     5041 tgagtggtaa taagttgggc tcaaacaaat gaaagtgatt tggaatggtg tagccaagga
     5101 aaaaagcgat tccacacaaa aaagccattc taatgcgatt gcttgcgtga ataaaaattg
     5161 gaagagtcat ggttacttag agtccgtttt gagcgctttc cttaattctt caacaattgc
     5221 gggagcagac caatcgcgct cgccgctttc tgcccaaagc actcgctggt cctttccgag
     5281 aacaaccgaa tagggaattg ccactacgct aaaaaaatca gttaatgtgt tttttggttc
     5341 caaatacact gggaacttca gctccaactt tttggctagg ttcggcacca cgtcattggg
     5401 attttcgtcg acattgagtg caatgacttc aaaaccttcg ttcttgaggg tttcgtagag
     5461 acgattgatg ctgggcattt caaccaaaca agcgctgcac caacttgccc aaaagttaat
     5521 taagacaacc ttgcccttaa acgcctcaag cgatacggct tgttgatcat gaaagcgcac
     5581 aaacgacacg ttgggcactt gacgctggtc agcagatttt gggttttcca atgatattgg
     5641 attttttaag cggaaatgtc gcgcgacgct tagtgttacg cca
//
LOCUS       DAOMXO010000054         5629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7625_length_5629_cov_6.094726, whole genome shotgun sequence.
ACCESSION   DAOMXO010000054 DAOMXO010000000
VERSION     DAOMXO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7625_length_5629_cov_6.094726"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..215
                     /locus_tag="PLH57_02015"
                     /pseudo
     CDS             1..215
                     /locus_tag="PLH57_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997945.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
     gene            373..2064
                     /locus_tag="PLH57_02020"
     CDS             373..2064
                     /locus_tag="PLH57_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRK01415.1"
                     /translation="MRGSFAPKRRVLGFSLIEMLIGMGTLAIVASAVGSLSFYYFRNQ
                     KANQLKNTYGDIVRRVSSTLKNPSAIRKSARNSAYEGNTNLLNCLYDEGSADNNNAND
                     CVQSAIPFPPATVLLTDPALSNQLSSLATRQLQIRLVDSVNQLIHGPGKTYWTAAGVP
                     CDGADGRPTPSSPASFCPIESGVFYQAACNPTNNLSQRATCEIATHFRFFYTVKIREG
                     ANVPGGALLRPYNSHLESTLDPIVYSTPDLVRLKIANARQCNTDNGEVMTGIDSSGDL
                     ICSKIETQKHLLARHSMRNSVPNCNFSVLRSGTTVPVAKSFPSAFASGVGGYSFAGAW
                     ISAQSGAVPDLGKPGSCLVVSNLSDIMSRVNGVARTDFFAEMDDDTPFRAWGTGNDFS
                     MWMRATNVTPPSVPLADFPNTAPYVSRCNVCEIDGPVIAVHNFNLATPDAPPACPAGW
                     DRLYTGRALAAMTLHASDGDSGFNHGTNLSSPGSCLPIAGTIDPIPFFECKHYTSDGD
                     WWGPGTPPQCQHRTPGDFALWYARDNARAFQCSVCYKPSRIEPQPLVWQAIVIGN"
     gene            2099..3802
                     /locus_tag="PLH57_02025"
     CDS             2099..3802
                     /locus_tag="PLH57_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01416.1"
                     /translation="MFNVAFRSPKKATQGLSLLELVISVGLAGVIALVASGILANLQR
                     AANHDKIRRTQLEIARLLSNTLKDSEAVVRTAQGAFTGNRNIRRCLYQSQWENTADCL
                     QRPYMRVIETNIVPIDVVQMSPQPVNFTTSAVYHFELRNASNQYVHPPGVARWTIDGV
                     LCDGRDGRPVYTYASRDACPIRSELAYVAACNDNLASEASCYRATDIKFVYWVSVASS
                     SLFGGGANFPTVDSTSMDPILQSVEKITRTEIVATGSCNLENGQVLAGVTTDRNLRCV
                     QVQKKKTLLARHSFSSSVPNCSFQVRMRNGNTFTNTQQTFPNAQPRLDGYSFAGAFTD
                     IRVGTNIDLGGAGSCVDVAATRWDQFTDGITIEIDDELEVRRNSSGDFTLWLANLSNP
                     PDGVSYGEDNENVVGARKISRCAVCEIPGEVSVVHNINRSNNTVPNCPDGWGQLYAGF
                     SFGSFTAHSDGFNAGYTANQDMASPGSCLQMEFPYHTPFIECHHTSDSSDRRCLHNTS
                     GDYAQFFGGRPMSTSESGRWSRCSVCYKPQKITQQPNDQLDSVSTQPLTTQPLTNVLG
                     G"
     gene            3810..5513
                     /locus_tag="PLH57_02030"
     CDS             3810..5513
                     /locus_tag="PLH57_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01417.1"
                     /translation="MSNGIKRRINLQGGFTALQSIIIAAAVALAVASGGQVVLQQIRQ
                     QKSENLKIGQKAVAERVRHYISSPEGLRVTSNKLWDILSFNTPSARATRTLKGCLTDD
                     GGTLDCLELAPNEWVTHLYVLNPLHELVHPLSGRTIMRQIGEYQFWKIDGTPCLDATG
                     ATRNCPIASRVLFRPVCHTENDSLFSCEVATHLVFRYEVTNPISPHTGDANGFRTAPI
                     SAEFVLTTEEINYVTPPTNPGCDTANGYVPTGITANGNFICGRPPVNTNLIARHYISG
                     TQAAPGCTFKNVVRPDGSRYNVSFKNVDSSLPARVTLPYAGNPTTRRSFAGLWGTYSF
                     GTQIDLGGHGSCINNSRFAAGFYTECSRSGSCQSPTLYDRTMWLRDVDRRLPESATIN
                     EATIPNLQTVLSECAVCEIEGHISVVHNFNKTDHRHLAPQCPAGYDRLYEGRSVAAFS
                     GAGRGNFQGYTVRHDLSSISSCHPYNAETVPFVDWLTDSQAESWKLYTHGRMPFIEHY
                     SSSNTFGHLSIAQYTAYFGRQIEQTTNNWTTRTTAPFKCSVCWKPPGVSENILSTNIG
                     N"
BASE COUNT         1444 a         1494 c         1331 g         1360 t
ORIGIN      
        1 ctggcggact ctctggagca actcccagtg aagccgttag ctggggcaaa gtagaccccg
       61 atcaattgcc cgacaccgtg gtctgctacg gcgacactac gatttcgctg ccgctcgtta
      121 ccgcctacgc gcttgaccgc tgcaaaccac gaaaactcaa aaggctgtac gataaacgtg
      181 aagcactctt tgaaaaaatg cagaaagctt acctaaaacg cattgaatga cacacccaag
      241 tgggggaccg attcaaaagt gtgccaaacc cgttaagaaa atttaattac caactaaccc
      301 cctgaaattt ttgggctgtt gacgctttca attcccgata gaactgctaa acttcttgat
      361 tagggggcca atatgagggg gagtttcgca ccaaaacgtc gagttttggg tttttcgctg
      421 atcgaaatgc ttataggcat gggcacactg gctattgttg cttcagcggt tggttcgtta
      481 agcttctatt attttcgaaa ccaaaaggcc aatcagctta aaaacactta cggcgatatt
      541 gttcgccgag tctcctctac actaaaaaat ccatcagcga ttcgaaagtc tgctcgtaac
      601 agcgcctacg aaggaaacac caatttacta aactgtctct atgacgaagg ttcagccgac
      661 aacaataatg caaacgactg tgtgcaatcg gccattccgt ttccgcccgc aactgttttg
      721 ttaacggatc cagcgcttag caaccaactt tcttcgctcg ccactcgcca acttcagatc
      781 cggttggttg attcggtcaa tcaattgatt catggtccgg gaaaaactta ctggaccgca
      841 gccggcgtgc cctgcgacgg cgccgacggc cgaccaacac caagctcccc agccagtttt
      901 tgcccgatcg agtcgggggt cttttatcaa gcggcttgca acccaaccaa taatctttcg
      961 caaagagcga cctgtgagat cgctactcac tttcgctttt tctatactgt gaaaattcgc
     1021 gagggagcaa acgtccctgg cggcgcgctt ttgcggccat acaacagcca tcttgaatct
     1081 acattagacc cgattgtcta ttctacgccc gacctcgtgc gactaaagat agcgaacgca
     1141 cgacaatgca atacggataa tggcgaagtc atgaccggta tcgacagcag tggcgatttg
     1201 atttgctcga agattgagac tcagaaacat ttgttggctc gccactccat gagaaatagc
     1261 gttccgaatt gtaatttcag cgttctgcgt tcaggaacca ccgtaccggt tgctaaatca
     1321 tttccgagtg cctttgcttc tggtgttggt ggttacagtt tcgcgggtgc ttggattagc
     1381 gcccaaagcg gtgcagttcc agatttggga aaaccaggct cgtgcttagt cgtttcgaat
     1441 ctcagcgaca ttatgtcccg agtaaacgga gtcgctcgaa ccgatttttt tgctgaaatg
     1501 gatgacgaca ctccgtttcg ggcttggggc actggaaacg atttttcaat gtggatgcga
     1561 gccaccaacg taactccgcc tagcgtgccg ctagcagatt ttccgaatac cgctccatac
     1621 gtaagtcgat gcaacgtctg tgaaatcgac ggtccagtga ttgccgtaca taacttcaac
     1681 ctagcaacac ccgacgcacc gcctgcttgt cctgccggat gggaccgtct ctacacaggg
     1741 cgcgctctgg ctgcgatgac gttacacgca agtgatggcg acagcggctt caatcacggt
     1801 acaaacttaa gtagtccagg cagttgcctg ccaatcgcgg gaacgattga cccgattccg
     1861 ttttttgaat gcaaacacta cacaagtgat ggcgactggt gggggccagg cacgccaccg
     1921 cagtgtcagc accgtacgcc gggggatttt gcactctggt acgcacgtga caacgctcgc
     1981 gcatttcaat gttctgtttg ttacaaacca tcgcgaattg agccacagcc acttgtttgg
     2041 caggcgatcg tgattggtaa ctagatttta ttagaagggg ttcacggggt aacaaggtat
     2101 gtttaatgta gccttccgct caccaaaaaa agcgacccaa ggtctctcac tacttgagct
     2161 cgtcatatcg gtaggtcttg ccggcgtaat agcgctggtg gcctctggaa tattggcaaa
     2221 cctgcaaaga gctgccaacc acgacaaaat tcgtcgcact caattagaaa ttgcgcgcct
     2281 tctttccaac acactcaaag attccgaagc agttgtaaga acagctcaag gtgcgttcac
     2341 tggaaaccgc aacattcgtc gctgtttgta tcaatcgcag tgggaaaata cggctgactg
     2401 tttacaaagg ccttacatgc gagtcatcga aaccaacatc gttccgatcg acgttgtgca
     2461 aatgtcaccc caacccgtaa acttcacgac ctcggccgtt tatcatttcg agctacgaaa
     2521 tgcttcgaac caatacgtac accccccggg tgtcgcacgc tggacgatcg atggtgttct
     2581 gtgcgacggc cgagacgggc gacctgtcta cacctatgcg tcgcgggacg catgccctat
     2641 tcgctctgaa ctcgcctatg tcgccgcatg caacgacaac cttgcgagtg aggcttcgtg
     2701 ttatcgggca accgacatta aattcgttta ttgggtgtcg gtcgcaagtt cttcgctctt
     2761 cggcgggggc gcaaattttc caacggtgga ctctacatca atggacccaa ttcttcagtc
     2821 ggttgaaaaa atcactcgaa ccgaaatcgt cgccaccgga tcgtgtaatc tagaaaatgg
     2881 gcaagtactg gccggcgtca cgacagaccg caatttacgt tgcgtgcaag tgcaaaaaaa
     2941 gaaaacgcta cttgcccgcc actcatttag ctcatcggta ccaaactgct cctttcaagt
     3001 tcgaatgaga aacggcaata cgtttaccaa tactcaacaa acgttcccca atgctcagcc
     3061 gagattagat ggttacagtt tcgccggcgc attcaccgac attcgagtag gcactaacat
     3121 cgatttgggt ggtgccggat cttgcgtaga cgtcgcggca actcgctggg accaattcac
     3181 tgacgggatc acgatcgaaa tcgacgatga attggaagtt cgccgcaatt cgagcggtga
     3241 ttttacgctc tggctggcta atctcagcaa tcctccagac ggagtgagct acggtgaaga
     3301 caacgaaaat gtagtcggtg ctagaaagat aagccggtgt gccgtttgtg aaattccagg
     3361 cgaagtgtcg gtagtgcaca acataaatag atctaacaat acggtgccaa attgcccgga
     3421 cggttggggc caattgtatg ctggctttag tttcggctcg ttcactgccc actctgacgg
     3481 gtttaatgct ggttacaccg cgaatcagga catggctagt cctggcagct gccttcaaat
     3541 ggagttcccg tatcacactc cattcatcga atgtcatcac acgagtgatt ccagtgatcg
     3601 tcgctgctta cacaatactt cgggtgacta cgctcagttt tttggtggac gccccatgtc
     3661 gacgtccgag tctggtcggt ggtcgcgttg ttcggtctgc tacaaaccgc agaaaattac
     3721 ccagcaacca aatgatcaac tcgatagcgt ctcaacacaa cctctcacaa cacagcctct
     3781 aacgaatgta ttgggtggat agggaattta tgagtaacgg aattaaaaga agaatcaatt
     3841 tacagggcgg gttcacggca ttgcagtcaa tcataattgc cgcagccgta gcgctcgcag
     3901 tagcgagcgg aggccaagta gttcttcaac aaattcgcca acaaaaaagt gagaacttaa
     3961 aaatcggcca aaaagcggtc gcagagcgag ttcggcacta tatttcttcg cccgaaggtt
     4021 tacgggttac ctcgaataaa ctctgggaca tcttaagttt taacacacca agcgctcgcg
     4081 ccaccagaac actcaagggc tgtttaacgg acgacggtgg aacgctcgac tgcctagaac
     4141 tcgcaccaaa cgaatgggta acccaccttt acgtactcaa tcctcttcat gagctagtgc
     4201 acccactctc tggtcgaacc atcatgcgcc aaattggcga ataccaattt tggaaaatcg
     4261 acggaacccc ctgccttgac gccacgggtg ccactcggaa ctgcccaatt gcttcacgtg
     4321 ttttgtttcg gccggtctgc cacaccgaaa atgattctct tttttcctgt gaagttgcca
     4381 ctcacctagt ttttcgctac gaggtaacaa atcctatttc tccgcatact ggcgacgcaa
     4441 acggctttcg tacggcgccc atttcagcgg aattcgtact tacgacagaa gaaattaact
     4501 acgtaacacc acctacgaac ccgggctgtg acaccgcgaa cggttacgta ccgactggaa
     4561 ttacagcaaa cggcaatttc atttgcggcc gcccgcctgt gaacactaac ctaatcgcac
     4621 gccactatat ttcgggcacc caggccgctc ccggatgcac ctttaaaaac gtggtgcgcc
     4681 ccgacggatc acgatacaac gtaagtttta agaacgtaga ctcgtcgttg cccgcaagag
     4741 tgaccttgcc ttatgccggc aatcccacca ctcgtcgtag ttttgctggg ctctggggta
     4801 cttatagttt cggaacacaa attgatctag gcggacacgg ttcgtgtatt aacaacagtc
     4861 gctttgctgc tggtttttac accgaatgca gtcgatcagg tagttgccag tctcctacac
     4921 tgtacgaccg caccatgtgg ttgcgcgacg tggaccgcag actaccggag tcggccacta
     4981 tcaacgaagc gaccattcca aacctacaaa cagtactcag tgaatgtgcc gtgtgtgaaa
     5041 ttgaaggcca catatccgtg gtgcacaact ttaacaaaac agatcaccgg catttggcac
     5101 cgcagtgccc ggcgggctac gatcgtttat acgaaggtcg ctcagtggcg gccttttcgg
     5161 gtgctggtag aggcaatttt caagggtaca ccgttcgaca tgatctcagc tccatttcaa
     5221 gctgccaccc ttataacgcc gaaactgtgc cctttgtgga ttggttaacc gacagtcagg
     5281 ccgaaagctg gaagctatac acccatggac gcatgccttt catcgaacac tattcgagct
     5341 ccaacacctt tggtcatttg agcatcgcac agtacacggc gtactttggt cgccagatcg
     5401 aacaaaccac taacaactgg accactcgta ctactgcacc gttcaaatgc tctgtgtgct
     5461 ggaaaccgcc tggagtttca gaaaatatcc ttagcaccaa tatcggaaat taataaataa
     5521 tttcaaatag ttaaagccaa atttattact taatcaaaaa gatcaactaa tggtataata
     5581 tccggtgagg ggtgttttta tgtttaagca aaggggtcgc ttcgcctcc
//
LOCUS       DAOMXO010000055         5618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7649_length_5618_cov_5.247528, whole genome shotgun sequence.
ACCESSION   DAOMXO010000055 DAOMXO010000000
VERSION     DAOMXO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7649_length_5618_cov_5.247528"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLH57_02035"
     CDS             complement(<1..455)
                     /locus_tag="PLH57_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRK01418.1"
                     /translation="MPENNLKRQLELSRFERALEVVNSMVESGVFLNTAELGRLNQIL
                     TGTQNDPWRVEPAICKLPSGREATFAILADPIKKAREILRAAKDRADGGEAIEAAADV
                     YAQFVLTHVFKDGNRRTAVVAAYFLLKAHGHSVSAVGLHELGLGDLRID"
     gene            complement(492..779)
                     /gene="spoVG"
                     /locus_tag="PLH57_02040"
     CDS             complement(492..779)
                     /gene="spoVG"
                     /locus_tag="PLH57_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HRK01419.1"
                     /translation="MEITEIKIFPVNEEKLKAYVTIILDNCFVVRDLKVINGNTGLFV
                     AMPSKKRKDGVYKDIAHPLNTETREKMEAQILAAYDSELKAGGGRRPSDED"
     gene            complement(878..3466)
                     /gene="clpB"
                     /locus_tag="PLH57_02045"
     CDS             complement(878..3466)
                     /gene="clpB"
                     /locus_tag="PLH57_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRK01420.1"
                     /translation="MAAKFTAKTEEALVDAQHLAIRRNHQEIQPEHLLVALLKQPEGI
                     VPSIVRTAGADPEFIEKEISAALGKFPSVTGAATEGKVYPSGAFNKLLVLAEDEATKL
                     KDEYVSVEHFLLGFLGNRNAKDSDAYKAFFKADVNYEKVQTILKKIRGGARVQDENPE
                     AKMNALEKYGRDLTAVAAKGKLDPVVGRDEEIRRVIQVLSRRTKNNPILIGEPGVGKT
                     AIAEGLAQRVMKGDVPEGLKNKRVIALDLGALIAGAKFRGEFEERLKAVLKAVNDSAG
                     SVILFIDEIHTLVGAGASEGAMDAANLLKPALARGELKCVGATTLNEFKRIEKDAALE
                     RRFQPVFVKEPTVEDTVSILRGLREKYELHHGVTIKDGALVSAANLSNRYIADRFLPD
                     KAIDLIDEAASRVRTALDSVPEEIDVLDRKILQAEIERQALKKETDARSKDRLEKIET
                     ELRDAKEKSKTLRAQWQKEKQEVQGLGELKSEIEQKRFEMEQAEKRGDLEKAAELKYG
                     VLLDLQKKLDERTAKAKERKILHQEVDERHIAEVVSKWTGVPVSRMLESEVQKLLQME
                     KRMHERVVGQDAAIEAVSDAIRRSRAGLQDPRRPIGSFLFLGPTGVGKTETAKALAQF
                     MFDDEHAMVRIDMSEYMEKHSVARLVGAPPGYVGYEEGGQLTEAVRRRPYSVVLLDEI
                     EKAHGDVFNIFLQILDDGRVTDGQGRTVDFTNTVIIMTSNIGSREILSEKQPRKREEI
                     VMQVLRNHFRPEFLNRIDDTVIFNSLDETQITEIVRIQTAKLAELLAAKELRFSISDE
                     AVKYFSKTGYDPDYGARPLKRLIQKELQNVVAKKLLEGAFLPGDTVSVGLKGGSLYVE
                     KNAASQ"
     gene            complement(3512..5071)
                     /locus_tag="PLH57_02050"
     CDS             complement(3512..5071)
                     /locus_tag="PLH57_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRK01421.1"
                     /translation="MNKTMKIRAILGLSVLAIWGLTKELHAASADNVVTPVNQVFIQI
                     SKKMIPSVVNIFTTSTVKAPHWGDRGRTPGQPGDELWRRFFEEFLGEPFGGGVMPGPR
                     GDGNRVMPQRKAMSLGSGFVIEKNSVGGLILTNNHVVEGADEIKVKFTEDVEEKESEA
                     EIIGRDRELDIALLKVKTRRTLQPVVLGDSDKLEVGEWIAAVGNPFGHGHSVSHGIVS
                     AKERSLPGTFGNYLQVDAPINPGNSGGPLVNLSGEVVGINNAIDARGPGIGFAIPVNM
                     LKKVLEQLKTKGRVDRGYIGVNVQEFNSEIAKALKLDEDRRGPIVSDVVGGKPAARAG
                     IKKSDVITEVAGKRVRTIGELLNTITNIPIGEKADVTVLRSDDGKVSEKKFKVDVEGR
                     PDFRADAKGRHDNKGPDRPGKAEVAGITVEQIDSEIAGELGLPADYQGVVIKEIKPDS
                     PGIEAGLERGDVIVDIKEAGKETRPVKKVEDFVRMLKGKKRFVLGIRTTDPDGEEVFE
                     VRPLNLTGKKD"
     gene            complement(5085..5330)
                     /locus_tag="PLH57_02055"
     CDS             complement(5085..5330)
                     /locus_tag="PLH57_02055"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HRK01422.1"
                     /translation="MKLDFTTFVLSISSAVFMGLGLTPRPDSKEKKVDLELARHNIDL
                     LELIKEKTKGNLSSDEQKLIDNLLYETRMRFVEAQKK"
     gene            complement(5327..>5618)
                     /locus_tag="PLH57_02060"
     CDS             complement(5327..>5618)
                     /locus_tag="PLH57_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRK01423.1"
                     /translation="QTLVARSKDFEADRAGAEFCMQPEWMANALRKIQNMSTGHATFG
                     TPGAAHLYTVNPYSSSALLGVLDRHAPVELRVQKLLALKYGKYRSIQSGVQL"
BASE COUNT         1300 a         1489 c         1173 g         1656 t
ORIGIN      
        1 ctatcgattc taaggtcacc gagaccaagt tcgtgaagcc caacggctga aacgctatga
       61 ccatgggctt tgagtaaaaa ataggccgcg acaacagcgg tacgtcgatt tccgtcttta
      121 aagacgtggg taagaacgaa ctgggcataa acatcggcgg cagcttcaat cgcttctccg
      181 ccgtctgctc gatctttggc tgccctcaaa atttctcgag ccttttttat cggatcggca
      241 agaattgcaa acgtggcttc gcgtcccgaa ggcagcttac atatggcggg ttctacgcgc
      301 caaggatcat tttgagtgcc ggttaatatt tggttcaatc gtcctagttc cgcggtattt
      361 agaaacacgc cggactcgac catactattg actacctcga gcgcgcgctc gaagcgggaa
      421 agttctaatt gtcgttttag attattctcg ggcatagccg gaaactagca taaggacggg
      481 ggggcgaaaa tttaatcttc gtcggacggg cgtcggccgc cacccgcctt cagctcggaa
      541 tcataagccg ccaatatttg cgcttccatt ttttcccgag tttcggtgtt tagtgggtgt
      601 gcgatatctt tataaacgcc gtcttttcgc ttttttgaag gcatcgcaac gaatagcccg
      661 gtattaccat tgatgacctt gagatcccgg acgacaaaac aattgtcgag aataatggtt
      721 acgtaggcct taagcttttc ctcattaacc ggaaaaattt ttatttcggt gatctccatg
      781 ttgcctctcc tggtcactgc acctacaaaa gaaataccct actatttcca ttgtaaaccc
      841 agactagaac acgacgcaac aaagcggctt aaatcagcta ctgcgatgcg gcattttttt
      901 ccacatatag cgagccgccc tttaaaccta ccgaaaccgt atcacctggc agaaaggcgc
      961 cttccaatag tttctttgcc accacatttt gcaactcttt ctgaataagc ctcttcagag
     1021 ggcgagcacc atagtcgggg tcgtacccag tcttcgaaaa atatttgact gcctcatccg
     1081 agatactaaa tcgaagctcc tttgcagcta acaactcagc cagtttcgcg gtctgaatcc
     1141 gtacaatttc ggtaatttgg gtctcatcta aactgttgaa aattaccgta tcgtcgatgc
     1201 gatttaaaaa ttcgggtcga aagtggtttc ttaagacctg catcacaatt tcttcgcgtt
     1261 ttcttggctg tttctctgat agaatctcgc gactgccgat gttactcgtc ataattatca
     1321 cagtgttggt aaagtccacc gttcttccct gtccgtcggt gactcgacca tcgtcgagaa
     1381 tctgtaaaaa aatattaaac acatcaccgt gggctttttc gatttcatcc aataaaacca
     1441 ccgagtaagg gcgacgtcgt acggcttccg taagttgtcc gccttcttcg tagcccacat
     1501 aacctggagg tgcgccaacc aagcgcgcta ccgaatgttt ttccatatat tcggacatat
     1561 caattcgtac cattgcgtgt tcatcatcga acatgaactg agcgagtgct tttgctgtct
     1621 cagtttttcc aacgccggtt ggtccaagaa acaaaaagga accgatcggc ctgcgcggat
     1681 cctgcaaacc agcacgagat ctgcgaatgg cgtcggagac agcctcaatc gctgcgtctt
     1741 gcccgacaac tctctcgtgc attcgttttt ccatttgcaa aagtttctga acttcacttt
     1801 cgagcattct cgacactggt acgccggtcc atttggaaac gacttcggcg atgtgtcgtt
     1861 catctacctc ttggtgaaga atcttacgtt cttttgcctt cgcagttcgt tcgtcgagtt
     1921 tcttttgaag atcaagcaaa acaccgtact taagctctgc ggctttttcc agatcgccac
     1981 gtttttcagc ctgctccatt tcaaagcgct tctgttcaat ttcgcttttt aattcaccca
     2041 atccttgtac ttcttgtttt tctttttgcc attgcgcccg aagggttttc gatttttctt
     2101 ttgcgtcacg cagttcggtt tcaatttttt ctaaacgatc ttttgatctt gcatcggtct
     2161 cttttttaag ggcctgacgt tcgatttcag cctgtaatat ttttcgatcg agtacatcga
     2221 tttcttctgg taccgaatcc aaagccgttc ttacgcgact agcggcttcg tctataaggt
     2281 cgattgcctt gtcaggcaaa aaccgatcgg caatgtaacg gttggagaga tttgcagcag
     2341 aaaccaatgc gccatctttt atggttacgc catgatgtaa ttcgtatttc tctcgtaggc
     2401 cacgtaagat tgaaaccgta tcttcgaccg taggttcctt tacaaagact ggttggaatc
     2461 gtcgttctag ggccgcgtct ttttcgattc gcttgaattc gttcaaggtg gtggcgccaa
     2521 cgcactttag ttcgccgcgg gccaaagctg gtttcaggag gttcgcggca tccatcgcgc
     2581 cttcacttgc tccggctcct acgagtgtgt gaatttcgtc tatgaaaagt atcacgctac
     2641 ccgcggagtc gttcaccgcc tttaaaactg cctttagccg ttcctcaaat tcaccacgaa
     2701 acttggctcc tgcaatgagt gcacctaaat ctagcgcaat gactcgcttg ttttttagtc
     2761 cttcgggtac gtcgcctttc atcactcgtt gggccaaacc ttctgcaatt gcggtttttc
     2821 cgacgcctgg ctcgccaata agaatggggt tattttttgt gcgtcgagat aacacctgaa
     2881 ttacccgcct aatctcttcg tcgcgtccca ccactgggtc taactttcct ttggcggcaa
     2941 ccgccgttaa atcgcggccg tacttttcta gagcgttcat cttggcttca ggattttcgt
     3001 cttgcacgcg ggctccccct ctaatttttt ttaggattgt ttgcactttt tcgtaattta
     3061 cgtcagcctt aaaaaaggcc ttataggcat cggagtcctt cgcgttgcga ttgcccaaaa
     3121 aacccaacag aaagtgctct acgctaacat actcgtcttt gagtttcgta gcttcgtctt
     3181 ctgccaaaac cagcagtttg ttgaaagcgc cggaaggata gaccttccct tcggttgctg
     3241 ctcccgtaac actgggaaat tttccgagtg cagcgcttat ttctttttca atgaattccg
     3301 gatccgcacc ggcagtgcga acaatagacg gtactatacc ttctggttgt ttcagcagag
     3361 caacgagcaa atgctcgggt tgaatttctt ggtggtttcg tcgaatggcc aaatgctggg
     3421 catcgactaa cgcttcttcg gtttttgctg taaattttgc agccatgtgc tttctcctca
     3481 aactggagga agcgaagccg caacctctca gctaatcttt tttgcccgtg aggtttaacg
     3541 gcctaacctc aaatacttcc tcaccgtcag ggtctgtcgt tcgaataccc aaaacaaatc
     3601 gtttcttacc cttcagcatt cgtacaaaat cttcgacctt tttcaccggg cgggtttcct
     3661 taccagcttc cttaatgtca acaatcacat caccgcgctc aagacccgct tcaattcctg
     3721 gactatcggg cttaatctct ttaatcacaa cgccttgata gtcagcgggc agacccaatt
     3781 cacctgcaat ttcagagtcg atctgttcga cagttattcc agcaacctct gcttttccgg
     3841 gacggtcagg acccttatta tcgtgtctac ccttcgcatc tgccctgaaa tcgggtcttc
     3901 cttctacatc gactttgaac ttcttttccg acacctttcc gtcgtcgctt ctcaaaacag
     3961 tcacatcggc cttttcgccg attggaatgt tcgttatcgt gttcagcagt tcaccaatgg
     4021 tccgaactcg tttacctgcg acttccgtaa taacgtcgga ctttttaatg cctgcccgag
     4081 cagccggttt accaccaacc acatcactaa cgatggggcc tctccggtcc tcgtcaagtt
     4141 ttagggcctt agcaatttca ctattaaatt cctgaacatt tacgccaata tagccccgat
     4201 caacccgccc ctttgttttg agctgttcga gcactttctt gagcatattc acgggtatag
     4261 cgaacccaat gccagggcct cttgcatcga tcgcattatt aatgccgacc acttcaccgc
     4321 ttaaattcac caacggcccg ccggaattgc ccggattaat tggcgcatcg acttgcagat
     4381 aattgccaaa agtacccggt agcgaccgct cttttgcact gacgattccg tgagaaacag
     4441 aatggccatg gccaaaagga tttcctaccg cggcaatcca ctcgcccact tccaatttgt
     4501 ctgagtctcc aagtaccacc ggctgtaggg ttcttcgggt ttttactttc aatagagcaa
     4561 tatcgagttc gcgatctcga cctatgattt ctgcttcact ctctttttct tcgacgtcct
     4621 ctgtgaattt aacctttatt tcgtcggccc cttcgacgac gtggttgttg gtaagaatca
     4681 aaccgccgac gctgtttttt tcaattacaa acccagaacc aagggacatt gcttttctct
     4741 gaggcattac gcggtttcca tcaccccgag gaccgggcat gacaccacct ccgaaaggtt
     4801 cccctaaaaa ctcttcaaaa aatcgtctcc ataattcgtc gcccggctgt ccgggtgttc
     4861 gccctcgatc accccagtgc ggcgccttta cggttgaggt tgtaaaaata ttcaccaccg
     4921 acggaatcat tttttttgaa atttgaataa acacttgatt gacgggagta acaacattgt
     4981 cagcgctcgc agcgtgtaac tctttcgtta agccccagat agcgagaacg gacaaaccta
     5041 ggatcgccct gatcttcatt gtcttattca tgcgtactcc gtatttactt tttttgtgct
     5101 tccacaaaac gcatccgcgt ttcgtataaa agattgtcta tcaatttttg ttcgtcagaa
     5161 cttaggttgc ctttggtttt ctctttaatg agctccaata aatcaatatt gtgccgcgcc
     5221 aactctagat caactttctt ttcttttgag tccgggcgag gcgtaaggcc caatcccata
     5281 aaaacggctg aagagattga caatacaaac gtggtaaaat cgagcttcat agctgcactc
     5341 cagattgaat acttctatac tttccgtatt tcagcgccaa tagtttttgt acccttaatt
     5401 ccaccggagc gtggcgatca agaaccccaa gcagcgcgga agacgaataa gggttcacag
     5461 tatatagatg agccgctccg ggagtaccaa aggtcgcatg accagtactc atattctgaa
     5521 tttttcgaag cgcattggcc atccattcag gttgcataca aaattccgct ccagcacgat
     5581 cggcttcaaa atccttcgat ctagctacaa gtgtttga
//
LOCUS       DAOMXO010000056         5608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7668_length_5608_cov_5.056726, whole genome shotgun sequence.
ACCESSION   DAOMXO010000056 DAOMXO010000000
VERSION     DAOMXO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7668_length_5608_cov_5.056726"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLH57_02065"
     CDS             complement(<1..294)
                     /locus_tag="PLH57_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRK01424.1"
                     /translation="MLISQTLSPIALRNGSKTAFRYLKFNVTYKEFWETANRYSYYLQ
                     KEWGHGLRVGLWMSSCPHIAYSFIALANTKSCSVPLNPWGSPEENLFKIKNAGI"
     gene            306..1052
                     /locus_tag="PLH57_02070"
     CDS             306..1052
                     /locus_tag="PLH57_02070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK01425.1"
                     /translation="MSVPFTTVILFFNKSALTLRCLKSVYQAGLSENSILLVDNGSTP
                     ETASSIHSSTKAPVLRLENNAGFAKGMNLGLRHCFSDLATQWVLALSNDTELQPSFEV
                     DFCNFLSEITNIEAPTILGPAVYHLQSKTLAYTHGRFSKELAGFSALSHQVAQEQRVP
                     IVPPFYYPAAATLWNRRAFEALGGFNERFYCYWEDVDLSYRCAQSGVRLLPAPGLHVY
                     HQGKGTTSGKKHYVSAFHQGEQTFRRLWDL"
     gene            1087..1983
                     /gene="hslO"
                     /locus_tag="PLH57_02075"
     CDS             1087..1983
                     /gene="hslO"
                     /locus_tag="PLH57_02075"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRK01426.1"
                     /translation="MDKWIKCIAKGGNVMATAITAKTLIDEAQKRHALAHPTNKSHPS
                     PVQALGESLIAGLLLSSNCKAGERISLSVKGEGFLKHVLVDALPDGSVRGFLISRAAE
                     VPPVDSTLGPWQKGLLSVVRLKLGEKEPYVGTVPIVTGHLAKDLTFYLLQSEQIPAAV
                     GLAVNFDRSGKVESAGGFLVQVVPGASKKEIARIEQNINKLQSLAREIAKNSDPTVLL
                     GQIFDDMTFIILDEKPLRFECSCSKERIVRALMLLGKDELRDMMSQDHGAKVHCDFCS
                     KLFDISESELDQIIKQLGAKAE"
     gene            complement(1970..2539)
                     /locus_tag="PLH57_02080"
     CDS             complement(1970..2539)
                     /locus_tag="PLH57_02080"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRK01427.1"
                     /translation="MAKVYTKVGDQGQTALLGGEKVSKADARIEAYGTIDELNSFIGH
                     ARAATGSDSKASPLDSWLEQLQHWFFDLGSLLAAGVAERERFTLIPITAKHTLWVEEK
                     IDQMTESLPELRQFILPAGSELATRLHLARTVARRAERAMVQYQASGHVLPEHAVPFI
                     NRVSDFLFVAARYANGSEPETVWKKSIPP"
     gene            2670..3467
                     /locus_tag="PLH57_02085"
     CDS             2670..3467
                     /locus_tag="PLH57_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01428.1"
                     /translation="MILRILGILKLGCLIAGPSAQAYQLISHEQMAHSALIHLKNYQP
                     YFQETAKWVRDATPDQFRALRAVLLRSSIEADLRPDLWGENILLGPANGGHSGSASVL
                     FTSVFHFIVIRENPDGTVIPGEFWKHHDGYSWRHSSQTGAFDSLLSLPTSRIRGDLSH
                     SLGGTTPAHVRLPFADIPAYKKNFRLSDSAFRRMFHSSSNRAIDAVFPPADVPAELAF
                     NHVMRSPRATRTTYEQWWSPIAVMSYGGKKTTPRRFTEMEERVIPAN"
     gene            3509..4036
                     /locus_tag="PLH57_02090"
     CDS             3509..4036
                     /locus_tag="PLH57_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01429.1"
                     /translation="MPHHTIGTLDFCHHELEEEMEKRVCGSQKTPDQSPFFKGTYEVP
                     TKNCQHLYDPDLVARMLKTYDFFRLDQPYGLSARLRALARTTHQWRFGTVNPDAPNAS
                     IDQALDFVATQLPNGQVYSGNRCRDLFKHRAVQEQIRTQFNLGVAASIMVIELAARNY
                     ENGTGKQRALRSTNH"
     gene            3993..4388
                     /gene="queF"
                     /locus_tag="PLH57_02095"
     CDS             3993..4388
                     /gene="queF"
                     /locus_tag="PLH57_02095"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRK01430.1"
                     /translation="MVQENKELYGARIIEEAQLERFENRTKVRRYQVEFSCPEFTCLC
                     PRSGFPDFATINIKYVPDQWCVELKSLKLYINKYRNMNVFHEDVVNIILEDLVKLLDP
                     HEIEVVGDFNVRGNIKTIIRADHRKSNDR"
     gene            complement(4392..>5608)
                     /locus_tag="PLH57_02100"
     CDS             complement(4392..>5608)
                     /locus_tag="PLH57_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRK01431.1"
                     /translation="SAPCRQINRALEVAKLGDLISVGPGDFDSFSLTGPLAGPEEKEL
                     VIRGHGNQTVIHRHGDEVAAAISLSQVSNIWIEELTLQIDPAGRRTGGPRGHSSSGAA
                     VEIVESSGVWLHGVSINGASSSGIRVTRSSKIDVERCFIGGAGTLSSGIRMKDAMLVR
                     VADSVFEGGEYGIQATAHELEDPLANTSITIERNRFLDQSQAAVYLGGVRHSSLVNNV
                     VIGAGQFGMRLVPGLGSGERAEAEYLIPAGIKIYHNTIVAGVTSEAALAVELGGIPSE
                     EIKVSNNILLSENTDLIGTLYWDGARDIRYVNSDYNLIVNVRAGTKTEKMAASEFARE
                     YRREKRSVTQLAGRSVASQFADYPGRNFELAPNSAAENRGNPVAPVRNDHLRRFRGNS
                     DQVDIGAYERKN"
BASE COUNT         1415 a         1494 c         1282 g         1417 t
ORIGIN      
        1 aattccggcg tttttaattt taaataaatt ttcttccggc gaaccccatg gattgagcgg
       61 aaccgaacag gacttcgtat tggcgagggc tatgaacgaa tacgcaatgt gtggacaact
      121 cgacatccac aaccccactc gcagaccgtg gccccattct ttttgcaagt aatatgaata
      181 acgattcgcg gtttcccaaa attccttgta ggtaacgttg aatttaagat agcgaaacgc
      241 tgttttagat ccatttcgaa gtgcaattgg agaaagtgtt tgcgagatta gcattccttt
      301 aaattatgag tgttcccttc acaactgtca ttctattttt caacaaatcc gcgctcacgt
      361 tgcggtgttt aaagagcgtt tatcaagcgg gattatctga aaattctatt ctgctcgttg
      421 acaatggttc gacacccgag accgccagta gtattcactc ttcaaccaag gctcccgttt
      481 tgcgcctcga aaacaatgcg ggtttcgcca agggcatgaa cctcggccta cgacattgtt
      541 tttcagattt ggcgactcaa tgggtattgg cgctctcaaa tgacactgaa ctccagccca
      601 gtttcgaagt cgatttctgc aactttttat cagagattac gaatatagag gcccccacaa
      661 ttctgggccc tgccgtttac catctccaaa gtaaaacctt ggcctacact catggccgtt
      721 tttccaaaga gctggccgga ttttctgcct tgtcgcacca agttgcccag gaacagcgag
      781 tgccgattgt tccgcccttt tattaccccg ctgccgccac tctctggaac cggcgcgcgt
      841 ttgaagcgct gggcggattt aacgaacgat tttactgcta ctgggaagac gtcgaccttt
      901 cctaccgctg tgcccaaagc ggggttcgcc ttctgcccgc acctggtttg cacgtctatc
      961 accaaggaaa aggaaccact tctggtaaaa agcactacgt ttccgcattt catcaaggcg
     1021 aacaaacatt tcgacgcctc tgggaccttt gacttgcagc tctacctcgc ataaaaagac
     1081 caaccaatgg acaagtggat taaatgtatc gccaagggcg gaaacgtcat ggcaactgcg
     1141 attacagcaa aaaccctgat agacgaggct cagaagcgcc acgccttggc gcacccaacc
     1201 aataagtctc atccatcgcc ggtacaggca cttggcgagt ctcttatcgc ggggctttta
     1261 ctttcttcga attgcaaggc cggcgaacgc attagtcttt cggtcaaggg cgagggtttt
     1321 cttaaacatg ttctggtcga cgccctgccc gacggaagcg taagaggttt tttaatctca
     1381 cgggccgccg aagtaccgcc cgtagatagt acgcttggtc cctggcaaaa gggacttctt
     1441 agtgtcgtac gcttaaagct tggagaaaaa gaaccgtacg ttggaactgt gccaatcgtc
     1501 actgggcatt tggcaaaaga cttaactttc tatctacttc agtcggaaca aattcctgca
     1561 gccgtgggcc ttgcagtgaa ctttgatcgc agcggtaagg tcgaatctgc cggaggtttt
     1621 ttagttcaag tcgtaccggg cgcttcaaaa aaagaaattg ctcgaattga acaaaacatc
     1681 aataaattac aatctcttgc tagagagatc gccaagaact ctgaccctac ggtcttacta
     1741 gggcaaattt tcgacgacat gacgtttatc atcctagatg aaaaacccct acgatttgag
     1801 tgtagttgtt cgaaggaacg catagttcgc gctttaatgt tattggggaa agacgaacta
     1861 cgagacatga tgtcgcaaga tcacggcgcg aaagtgcatt gcgatttttg ttcgaaacta
     1921 ttcgatattt cggaatctga acttgatcaa atcatcaaac aactgggtgc taaggcggaa
     1981 tagatttttt ccacaccgtt tcaggttccg aaccattggc ataccgcgcg gctacaaata
     2041 agaaatctga aaccctattg ataaacggca ccgcatgctc gggcaacacg tgaccgctgg
     2101 cttgatattg aaccattgcg cgttctgcgc gccgagccac tgtgcgcgca agatgcaacc
     2161 gagtagccag ttccgaacct gcgggcaaaa taaactggcg aagttctggc aaactctcag
     2221 tcatctgatc aattttttct tctacccata gggtgtgttt cgcagtgatc ggaattaacg
     2281 taaatcgttc acgctctgcc acacccgcgg ctagcaagct gcctaaatcg aaaaaccaat
     2341 gttgcagctg ctcaagccac gaatcgagcg gtgacgcctt tgagtcggac cccgtcgcag
     2401 cacgggcatg cccaataaaa gaatttaact cgtcaatcgt gccataagct tcgatccgag
     2461 cgtcggcctt cgaaaccttt tctccgccca gcaacgccgt ctgaccctga tctcctactt
     2521 tagtataaac tttcgccatt taagggtcat ttgaatggcg acggtacgtc tcgtcaactt
     2581 caaaaaaaat gcaggggttg ctcgcctcaa aaaacttggc tagagctatt tgctgcgtgg
     2641 ggacggtgtc aggtttgcga aattcgcatt tgattttgcg tattctcggg attctgaagc
     2701 ttggctgctt gattgcaggg ccaagtgccc aggcttatca acttatttct catgaacaaa
     2761 tggcgcattc agcgctaatt cacttaaaaa attatcaacc gtactttcaa gaaactgcaa
     2821 agtgggttcg agacgcgact cccgatcagt tccgcgcact aagagcagtg cttttgcgaa
     2881 gttccattga agcggacctg cgccccgacc tctggggcga aaatattctt ttgggtcccg
     2941 ctaacggcgg acattcgggt tccgcttccg ttttatttac cagcgttttt cacttcatcg
     3001 tcattcgaga aaaccccgac ggcaccgtga ttcccggtga attttggaag caccacgacg
     3061 gctatagctg gcgccacagt agtcaaacag gagcttttga ctccctactc tcgttaccga
     3121 cttccagaat tagaggagac ctctcccaca gcctgggggg cacaaccccc gcgcatgtgc
     3181 gcctgccgtt tgccgacatt ccagcttaca aaaagaactt ccgtttgagt gacagtgcgt
     3241 ttcgtcgaat gtttcacagc tcgtcaaatc gcgctattga cgctgtattt cctccagctg
     3301 acgtaccggc tgaactggca ttcaatcacg taatgaggtc tccccgagcc acccgcacca
     3361 cctacgagca gtggtggtca ccaatcgcag tcatgagtta cggaggtaaa aagacaacac
     3421 cacgccggtt cacggaaatg gaagaaaggg tcatcccagc aaactagggg ccctgggcct
     3481 tacgctgcac cttgcgcaag acctggccat gcctcaccac acgatcggca cgctcgattt
     3541 ttgccaccac gagcttgaag aagaaatgga aaaaagggtc tgcgggtccc aaaaaacccc
     3601 cgaccagagc ccctttttca aaggtactta cgaagttcca accaaaaact gccaacatct
     3661 ttacgatccg gacttagtcg ctcgcatgtt gaaaacctac gatttttttc gactcgacca
     3721 accctatgga ctctcagcaa gactgcgggc gctggcccgc accactcatc agtggcgatt
     3781 tggcacagtc aacccggacg caccgaacgc cagcattgat caagccctgg actttgtcgc
     3841 cacgcaactc cccaatgggc aggtgtattc ggggaatcgc tgtagagatc tttttaaaca
     3901 tcgggcggtt caggaacaga ttcgcaccca atttaatcta ggtgttgccg cgtcaattat
     3961 ggtaatcgag ctggcggcga ggaactatga aaatggtaca ggaaaacaaa gagctctacg
     4021 gagcacgaat cattgaagaa gcccaactag agcgtttcga aaatagaacc aaggtacggc
     4081 gctaccaggt cgagtttagt tgtccggagt ttacttgtct ctgcccccgc tcagggtttc
     4141 cggactttgc taccataaac ataaagtatg tacccgatca gtggtgcgta gagctcaaaa
     4201 gtcttaagct ctacatcaat aaatatcgca acatgaatgt ttttcacgaa gatgtcgtaa
     4261 acataatatt ggaagatctc gttaaactgc tagatcctca cgaaatcgaa gtagttggcg
     4321 atttcaatgt tcgcggaaac ataaaaacta tcattcgcgc agatcaccga aaatcaaacg
     4381 atcgctagtt tttaattttt acgctcgtaa gcgccaatat caacttggtc cgaatttcct
     4441 cgaaatcgac gtagatggtc atttcgaacc ggcgccaccg gattacctcg gttctctgcg
     4501 gctgaatttg gcgctaattc gaaatttcgg cctggataat ccgcgaattg gctcgcaaca
     4561 cttcgcccgg cgagttgggt gactgagcgt ttttccctgc gatactctcg ggcaaattca
     4621 cttgccgcca ttttttcagt tttggttcca gctcgtacat tgacgattaa gttgtagtca
     4681 ctgttcacgt atcgaatatc tcgcgctccg tcccagtaaa gggttcctat taaatccgtg
     4741 ttctcactta aaagaatgtt gttagacacc tttatttcct cgctgggaat accccccagc
     4801 tccacggcca atgccgcttc actcgtaact ccggcaacga tcgtgttgtg ataaatctta
     4861 atccctgcgg gtatcaaata ttctgcctcc gcccgttcgc cactacccaa tccgggtacc
     4921 aaacgcatac cgaattgccc tgcgcctatt acaacgttat tcacaaggct tgaatggcga
     4981 acgccgccta aatacacggc ggcttgagat tgatctaaaa acctatttcg ctctatcgtg
     5041 attgaagtat ttgctagcgg gtcctctagc tcatgtgcag ttgcctgaat tccgtattca
     5101 ccgccttcaa acacactgtc ggccactcgc accaacattg cgtctttcat gcgaattccg
     5161 ctcgataaag tgcccgctcc acctatgaag cagcgttcaa cgtcgatttt actggacctc
     5221 gtcacacgaa taccgctgct cgatgccccg ttgatcgaga ctccgtgcag ccaaacccca
     5281 ctggattcta cgatttcaac agccgctccc gacgacgagt gaccgcgcgg cccccctgtg
     5341 cgtcgccctg ccggatcgat ctgtaaggta agttcctcga tccaaatatt gctgacttga
     5401 gaaagagaaa ttgcggcggc cacttcgtcg ccatgtcggt gaataactgt ttggttccca
     5461 tgaccgcgaa tgacgagttc tttttcttct ggtccagcta gcgggcccgt taacgaaaaa
     5521 ctgtcgaaat cgcctggacc gactgaaatc agatcgccca gtttggccac ctcgagcgcc
     5581 cgattaattt ggcggcaggg tgctgaat
//
LOCUS       DAOMXO010000057         5605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7674_length_5605_cov_4.787207, whole genome shotgun sequence.
ACCESSION   DAOMXO010000057 DAOMXO010000000
VERSION     DAOMXO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7674_length_5605_cov_4.787207"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2073
                     /locus_tag="PLH57_02105"
     CDS             <1..2073
                     /locus_tag="PLH57_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HRK01432.1"
                     /translation="PVRSNILAICAYVMFVGWTCGSSAAEVPLTIIHTNDLHSHYRPE
                     KGPFNIGGVSRMATILDRIRDQNPNTILLDGGDFSEGHIYYNLGAGRASVQMMNALGY
                     DAIVLGNHDWYNGPRVLGDVIEKMPPEFSFLGANLKIPDGLAADEPHLVRTIKPYDIF
                     VRGGVKIGVIGIVNFELIYDEFVKPAQPDVGFPYVRRYAKELKEEHKVDLVVVLSHNN
                     LAYNQVIAGMRYVDVVVHSHDHDRYSRPIAVERNGKTALMVEAGAWGRFVGKLDLLVD
                     TEKKTWRLKNYDLIQVDATAHDHPTISNLVTTLELELESKYGNIFSDQVIDLGMDLRR
                     EGNFGESVITNVMADAYRAYTGADVSFESPKFASGELYSGPVSTADLMNVIAFIYNPV
                     RDRTWTLKTGEMTGRALEEVITYMAFGMQKFEMRGGSISGIRAIYDAGPKTPDRSFGE
                     TALSLGALKSLEINGEPVKSNKYYKVALPEGILETITFMHNDSSAKKRFEIRNLEDTG
                     AEQWQVLSWYLQQNAPLNPSKFSRSGRFIAIQPDLALFYEDIQVRRKNHEAAVRVSVR
                     NVGLTDAPAGRRLSVAYEKTPDNFVDDPNMQIVLEEFELPAIAAGAAASLTVNVTLPL
                     EFTEKNVRLYIYMDNTSNDAVHSNNRAWVMSMAQVEKVEKNEEELESVPVAPPAKSKK
                     KIRKKSKR"
     gene            complement(2089..3453)
                     /locus_tag="PLH57_02110"
     CDS             complement(2089..3453)
                     /locus_tag="PLH57_02110"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRK01433.1"
                     /translation="MQSAVTNHFRFSLKTLGCKANVYDSLVLERELMELGGVRDELDP
                     QVFLVNSCTVTAEADKQSRRELILARRQHPNTVTIMTGCSAEVANSNEKTSYVDHLVP
                     NHAKSKLRTIVAHSLGLELPVAASEQTSEVYWGQLPEFAGRTRAFLKVQEGCNDFCTY
                     CIIPYARGKSRSVPLEQVLTEINRLQVQGVREVVITGTNIADWGKEFSSSIEDLVDEI
                     LRRTAIPRIRLSSLDPSEISERLLTLLANEPRLMPHVHLSLQSPVSRVLRAMKRDYRS
                     ENVVQALEAIARAGDSRGEPIFVGMDIIAGFPSESVEEHCEGLELLKSLPWMRMHVFP
                     YSEREGTPALKIPGSVAPADRKARAAELMVLSDRRHEQFARSAIGHTTSQVLIETTHR
                     GSELESNFNYVVGHAPNYSRVLARVASNDNIEKFRNQFISVSIEDVIPKPHRDWTLTG
                     TAIL"
     gene            complement(3458..4639)
                     /gene="mnmA"
                     /locus_tag="PLH57_02115"
     CDS             complement(3458..4639)
                     /gene="mnmA"
                     /locus_tag="PLH57_02115"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRK01434.1"
                     /translation="MALPSAHNEKLKKRARVLMAMSGGVDSAVAAALLKTQGYEVIGV
                     HMQLWNQADAAQKKSGGSCCSIVDSNDARRVCDLLEIPFYVINARDQFKNDVIDYFVH
                     EYLQARTPNPCVLCNNKLKFSYLIKKADELKCEYVATGHYAKVVHSADGGETSLYRAT
                     DRSKDQSYFLFGLKQPALSRTLMPLGDLLKTNVRKMANTFGLAVADKPDSQEICFIDE
                     GGYKEFVENNSTDRYRPGGPIVTSEGHILGRHLGLFRYTIGQRKGLGLDRPEHQDFYV
                     IGFDIKQNALIVGPEEFLMKSALVATNCNWIGAVDFIKGIRATAKIRSRHEEAECVIT
                     LLQNDSVRVDFDQPQRAITAGQAVVFYQGDLVLGGGWIEGLVEPVTTRMRARFSAGAT
                     K"
     gene            complement(4691..>5605)
                     /locus_tag="PLH57_02120"
     CDS             complement(4691..>5605)
                     /locus_tag="PLH57_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01435.1"
                     /translation="KLRDQAFFPLVKKLGRVPSKSNALARRISGPGLASQHAYQIAPD
                     LALVAMLAQMERADLQYFREGVEREIEEPARALERFTAVFKAVFGENNGGQILAGSTP
                     AFLVRNSVSERKKDLEDLTLERDLMLEKMTRMPTSVYLAEADLNVLLAKAEQLATMFY
                     QDAMDGSWSERAIADFWSRRNLEKGDWMGVAKYELTAVFGPQILTPLERVDQTFTIAV
                     DSPTDVSNLVAGLYFGIHPVDGPQLPQPLPPTVATATTEGDLNEVPSRVRGNLLCREI
                     LMDPIARYEEATKSAQIQRIRHIIGKKE"
BASE COUNT         1415 a         1336 c         1452 g         1402 t
ORIGIN      
        1 cctgtgcgtt cgaatatctt ggcgatttgc gcttacgtga tgtttgttgg ctggacctgt
       61 ggcagttctg ccgccgaagt tccgttgacg attattcata ccaacgatct tcattctcac
      121 tatcgccccg aaaaaggccc cttcaatatt ggtggggtgt cgagaatggc gaccattctc
      181 gatcgaattc gcgatcagaa tcctaacact atattgttgg acggaggcga tttttccgaa
      241 ggccacattt actacaatct cggagcgggc cgagcctctg tccaaatgat gaatgcgctt
      301 ggttacgacg ccatcgtttt gggcaatcac gattggtata atggtccgcg cgtgttgggt
      361 gatgtaattg aaaaaatgcc accggaattt tcctttctag gagccaattt gaagattccc
      421 gacggattgg ccgccgatga accacattta gtaagaacca tcaagcccta cgacatcttc
      481 gtgcgtggcg gcgtgaaaat cggtgtaatt ggaatcgtaa acttcgaact tatttacgac
      541 gagttcgtaa agccagcgca gccagacgta ggatttcctt acgtacgtcg ctatgccaaa
      601 gaacttaaag aagaacacaa ggttgatttg gtggttgttc tctcgcacaa caacttagcc
      661 tacaaccagg tgattgcggg tatgaggtac gtcgatgtcg tagtgcattc ccacgatcac
      721 gatcggtatt cacgcccgat tgcagttgag cgcaatggca aaacagctct gatggtggag
      781 gctggagcgt gggggcgatt tgtcggaaaa ctcgatttat tagtagatac tgaaaagaaa
      841 acttggcgcc taaaaaatta cgatttgatc caagtcgatg cgacagcgca cgatcatccc
      901 acgatttcga atctcgtgac gactttggag cttgagctag agagtaagta cggaaatatt
      961 ttttcagacc aagtaattga tcttggaatg gatctgcggc gcgaaggaaa ttttggggag
     1021 tcggtgatta ctaacgttat ggccgacgcc tatagggctt acactggggc tgatgtcagt
     1081 tttgagtcac ccaagttcgc aagtggtgag ttgtactctg gacccgtgtc gactgcagat
     1141 ttaatgaacg tgatcgcgtt catttataat ccagtacgtg atcgaacgtg gacattaaaa
     1201 accggtgaaa tgaccggacg agccttagaa gaagttatta cgtatatggc ctttggtatg
     1261 cagaaatttg aaatgcgggg cgggagtatt tcaggaatac gggccattta cgatgcggga
     1321 cctaagaccc ccgatcgtag tttcggggag actgctttgt cgttgggggc actcaagtcg
     1381 cttgaaatca acggtgaacc tgtgaagtcg aataagtatt ataaagtcgc gttgcccgag
     1441 ggaatacttg aaacaatcac ctttatgcac aacgattctt cggcaaaaaa gcgtttcgaa
     1501 attcgaaacc tcgaagatac gggagctgag cagtggcagg tgctctcatg gtatctccaa
     1561 caaaatgcgc cgcttaatcc tagcaagttt agtcgcagtg ggcggtttat cgcgattcaa
     1621 cccgatttag cgctcttcta cgaagatatt caggtgcggc gaaagaatca cgaggcggcg
     1681 gtgcgagtga gtgtacgcaa cgtaggattg accgatgccc cggcgggtcg tcgcttgtcg
     1741 gtggcttacg agaaaacgcc agataacttc gtagacgatc ctaacatgca gatcgtgttg
     1801 gaggagttcg aattaccagc catcgcagct ggtgccgcag cctcgttgac ggtcaatgtt
     1861 acacttccgc tcgaatttac cgaaaaaaat gttcgtttat atatctatat ggacaacacc
     1921 tccaacgacg cggttcatag caataaccgc gcttgggtaa tgtccatggc tcaagtggaa
     1981 aaagtggaaa aaaatgaaga agagctagag agcgtacctg tcgctccgcc ggcaaagagt
     2041 aagaaaaaaa ttagaaaaaa aagcaaaagg taaaggcggg ttggccgttt agagaatcgc
     2101 ggtacccgta agagtccaat ctcggtgagg tttcggaatt acgtcttcga tactcacgga
     2161 aatgaactga ttgcgaaatt tttcaatgtt gtcgttagat gccactcgtg cgagcacccg
     2221 cgaatagtta ggcgcgtgtc ctacaacgta attgaagtta gattcgagtt ctgaaccgcg
     2281 atgtgtggtt tcaatcaata cctgcgaggt agtgtggcca atcgccgatc tggcaaattg
     2341 ttcgtgacgg cgatcggaaa gcaccatcaa ctcggcggca cgggccttgc ggtcagctgg
     2401 cgctaccgaa ccaggaattt tcaaggccgg agtgccctcg cgttcgctgt agggaaacac
     2461 gtgcatgcgc atccacggta aagatttaag tagctccaga ccctcgcagt gctcctcgac
     2521 actttcagat gggaacccag caataatatc catgccgacg aaaatgggtt cgccgcgcga
     2581 atcaccagcc cgcgcaatag cctcgagcgc ttgtacaacg ttctccgagc gataatcccg
     2641 tttcattgcc cgaagtacgc gactcaccgg actttgaagt gataaatgaa cgtgtggcat
     2701 caatcttggt tcattagcta gtaacgtcaa aagccgttcc gagatttcac taggatcaag
     2761 cgacgaaagt cttattcgcg gtatggcagt acgacggaga atttcatcga ctaaatcctc
     2821 aatggacgaa gaaaactctt ttccccaatc cgcaatgttg gttccagtga tcaccacttc
     2881 acgaacgcct tgaacttgca aacggttgat ctcggttaac acttgttcaa gggggacgga
     2941 gcggcttttg ccgcgagcgt aaggtatgat gcaataagtg cagaaatcgt tacaaccttc
     3001 ttgcacttta agaaacgctc gggtgcgtcc agcgaattcc ggaagttggc cccagtacac
     3061 ctcagaggtt tgttcggaag cggcaacagg gagctcaagc cccaaggaat gagcgactat
     3121 cgtacgaagt ttgcttttcg cgtgattagg cacgagatgg tccacgtaag aagtcttttc
     3181 gttggagttt gcgacctcgg ccgaacatcc ggtcatgata gtaaccgtat tcgggtgttg
     3241 gcgtcgcgca aggatgagtt ctcggcgcga ctgcttgtca gcctcagcgg tcaccgtaca
     3301 gctgttgact aaaaaaacct gcggatcaag ttcgtcacgc acaccgccga gctccattag
     3361 ttcgcgctct aaaaccagtg agtcataaac attggccttg cagcccaaag tcttgagaga
     3421 aaatcggaaa tgattcgtta cagcagactg catggattta ttttgtcgca ccggccgaaa
     3481 aacgtgctcg cattcgagtt gtcactggtt caacgaggcc ttcgatccag ccgccgccta
     3541 gtacaaggtc gccttgatag aagacaacag cctgaccagc agtgatcgcg cgttgaggtt
     3601 gatcaaaatc aacacgaaca gaatcgtttt gaaggagcgt aatcacgcac tcggcctctt
     3661 catggcgaga gcggatctta gcagtggccc gaattccttt aatgaaatct actgcaccga
     3721 tccagttgca attggtcgcg acaagagcgg atttcatgag aaattcctct ggccctacga
     3781 tcaaagcatt ctgtttaatg tcgaaaccaa taacataaaa gtcttggtgt tccggccgat
     3841 ctagacctaa ccccttgcgt tgaccaatag tgtagcgaaa caaacccaag tgtcggccga
     3901 gaatgtgacc ttcggacgtg actatcggtc caccgggacg gtagcgatca gtagaattgt
     3961 tttctacgaa ctccttgtaa ccgccttcgt cgatgaaaca aatttcctgg ctatctggtt
     4021 tatccgccac cgctaaaccg aaagtgttcg ccatctttcg cacattggtt tttagaaggt
     4081 caccaagagg catgagcgtt cggctcaacg ccggctgttt taatccgaat aaaaagtaac
     4141 tttggtcctt cgaacggtct gtcgcgcgat acagcgaagt ctctccccca tcggcactgt
     4201 gaaccacttt ggcgtaatgg ccggttgcta catattcgca cttgagttca tcggcttttt
     4261 ttatgaggta gctaaattta agtttgttgt tgcacaacac gcaggggttg ggggtgcggg
     4321 cttgcaagta ctcgtgtaca aaataatcga tcacgtcgtt cttaaactga tcgcgtgcat
     4381 tgataacgta aaacgggatt tctagaagat cacaaacacg gcgagcgtcg tttgaatcca
     4441 caatcgaaca gcaagaaccg ccagatttct tttgagcagc gtcagcctgg ttccatagct
     4501 gcatgtgcac gccgatgact tcgtaaccct gagtcttaag cagagcggcg gccactgcgc
     4561 tatctacgcc gccactcata gccatgagga cgcgagcgcg tttcttgagt ttctcgttat
     4621 gggctgaagg taatgccata ctcccgagtg tatagcatac tccgtgcgcc gataagacgc
     4681 ttttttagag ttactctttc ttacctataa tgtgccgaat cctttgaatt tgtgcggact
     4741 tagtggcttc ctcatagcgg gcgatggggt ccattagtat ttcccggcat agcaaattgc
     4801 cacgaacccg gctgggaacc tcattaaggt cgccttcggt ggtggccgtg gcgaccgttg
     4861 gtgggagcgg ttgcggcaac tgcggcccat cgacagggtg aatgccaaag tagagacctg
     4921 cgactagatt cgatacatcc gttggtgaat cgacagcgat ggtaaacgtt tggtctaccc
     4981 tctccagtgg cgttagaatc tgcggtccga agaccgccgt cagttcgtac tttgctacgc
     5041 ccatccaatc acctttttcg aggtttcgtc gagaccagaa atcggcaatg gcgcgttcgg
     5101 accaggatcc gtccatcgcg tcctggtaaa acatggtcgc aagctgttca gccttggcga
     5161 ggaggacatt taaatcagct tcggcaaggt acaccgaagt gggcatgcga gtcatttttt
     5221 ccaacatcaa atcacgttcc aaagtgaggt cttctaggtc ctttttgcgt tcactcacag
     5281 agttgcgaac caagaatgca ggcgttgatc cagctagaat ctgtcctcca ttgttttcac
     5341 caaacactgc tttgaaaacc gcagtaaaac gctcgagggc tcgtgcgggc tcttcgattt
     5401 ccctttctac gccttcgcga aaatactgga gatcggccct ttccatttgg gcgagcatgg
     5461 ctacgagagc tagatcggga gcgatctgat atgcgtgttg agacgcaagt ccggggcccg
     5521 agatgcggcg agccagtgcg ttcgattttg atggcactct gcccagtttt tttactagtg
     5581 ggaaaaatgc ctgatcacgt agttt
//
LOCUS       DAOMXO010000058         5567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7728_length_5567_cov_4.883890, whole genome shotgun sequence.
ACCESSION   DAOMXO010000058 DAOMXO010000000
VERSION     DAOMXO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7728_length_5567_cov_4.883890"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PLH57_02125"
     CDS             <1..1037
                     /locus_tag="PLH57_02125"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRK01436.1"
                     /translation="IQNGARVILMSHLGRPEGVDSAFSLAPAADHLSELLGTDVLLTD
                     DCVGDGIEMIVRHLKSTQVILLENLRFHEGEEKNDPAFARQLASLAEVYINDAFGSSH
                     REHASIYGVPQIMPIKGCGFLIKKELQFLNRLLNAPEHPYVAVLGGSKVSDKIKTIEN
                     LFFEVDVLCVGGAMAHAFRLAGDPKHVLPPNAKQPKSQDIAYASQLLEKARRHEVKLI
                     LPIDDPEGVPPGFDIGPQTIELFTKEIRRAKTVFWNGPVGMFEDERYAKGSLAVAQAM
                     AEVKGLKVVGGGDTVSALNLAGVADQMTHVSTGGGATLEFLEGNGLPGITILDTIGKK
                     SDARISLVPE"
     gene            1034..1831
                     /gene="tpiA"
                     /locus_tag="PLH57_02130"
     CDS             1034..1831
                     /gene="tpiA"
                     /locus_tag="PLH57_02130"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRK01437.1"
                     /translation="MKSRKPILAGNWKMNLGPAEAGLYFQQLETLLSNDPTRSLDSER
                     VIFPPAYNLGSEIQWVAGKCQVQLGAQNIHWESTGAFTAELSAKALKDLGLNWALVGH
                     SERRQLFGETNQTAGKRLLKGLSSGLRVLFCMGETLGERESGRMETVWAEQLTSYVEA
                     LAKHVASDQANDFSSRHAPVAYEPVWAIGTGKTATTEQAEEAHRWIRNWLSEKLGATF
                     AEALPVLYGGSVTPDNAAALLKQPNIDGVLVGGASLKPESFAKILLA"
     gene            1873..2217
                     /gene="secG"
                     /locus_tag="PLH57_02135"
     CDS             1873..2217
                     /gene="secG"
                     /locus_tag="PLH57_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRK01438.1"
                     /translation="MGTFLIIFHVITCIVLVFVVLIQAGKEGGLGAMSGGSQTVFGSS
                     GGANFFTKFTAVTAALFMATSITLTVIKSGKKTSVFDGSESSAPIETTTSPATNAPAG
                     AAPSAPAGETTK"
     gene            2223..3158
                     /locus_tag="PLH57_02140"
     CDS             2223..3158
                     /locus_tag="PLH57_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01439.1"
                     /translation="MLQLQYHSLVELNFPNREWMWLRKPVLPSEQTTGAPDFYLLGVK
                     CDQRSFVWTRNGAALTDTNQGASITVPVLFFKPVDDELTAYLTYVKTAFQAVQPLEVE
                     WVRVLKTLKEQFQVDLAKGIPALLAATLANAGLEAHLGVLANRAKYTFLTSLELEFLY
                     EKKWDPSVFELFESISVELREAALKALQTWSISAANAKDLLQSLLFLSRKTSAETAIK
                     ILSSTYKTGDDLRIAIFHSAQPELARLSRERIERLRALSVPPRTSVFGDPSFESDQLK
                     ISHNPKTIGDFEAFKQWVSDPTTTERLRQLLEIYQ"
     gene            3162..4268
                     /locus_tag="PLH57_02145"
     CDS             3162..4268
                     /locus_tag="PLH57_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01440.1"
                     /translation="MIAKFERITASSEDILTTPAARRILKNLRRYSMLPDNFESPEII
                     SADRLSQELAFRATSQPAPYNTAVALGKKVLHLTTQPGTSVKQCPGTNNMLCCHYHII
                     NMVSNCPFDCSYCYLQTYLNQTLTTFFVNEDDIFEQVRNICKRPPVSLLRIGTGELSD
                     SLALDHLTEFSGRLANIMGEYQNVRLELKTKSKNVEHLLSIPKKDHVIISWSMNPPII
                     VEREEHGTARFDERIRAAAMAAGAGFRVGFHFDPLIYFDGWKDHYSAALEKLLAEVPH
                     RSIDWISLGALRFQPQMRAIALKRRPETQIFQEESVTGADGKVRYLKEIRSEMFGFIN
                     KKIKDLAPSIYTYLCMETKPVWEKALGRLPDRGF"
     gene            complement(4275..5192)
                     /locus_tag="PLH57_02150"
     CDS             complement(4275..5192)
                     /locus_tag="PLH57_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK01441.1"
                     /translation="MSSPGGITNVGFTQKRAAAEVVVCSALWGFGFIATRWAMDSFDA
                     FFLTGLRFLLAALLLFILAVSVPRWRKSLSLDEFFLAFLPGLFLSATLVLQTVGLRYT
                     TVTNSGFITVLYVVFVPVLEAVFFSRKTKLPHIGAILLALGGAGLLCQAWNLHNLNIG
                     DFLTLLCALAAAVQIVLVERASTKFQSPFLFNFYQSFWVGIIPFAVGIAMDSKNLTHA
                     PFAAHSLSWAGLAFLVVGSTMIAFLIQMRAQRVLPSATASMLFLLESPFAAVFGYFIF
                     NERMNGLQWTGAALIMIAAFVSARFGIKK"
BASE COUNT         1490 a         1435 c         1328 g         1314 t
ORIGIN      
        1 cgattcaaaa cggagcgcga gtgatcttaa tgtctcatct tggtcgccca gagggggttg
       61 actccgcttt ttcattggca ccagcagccg atcatttaag tgaattgcta ggcaccgatg
      121 ttctgctcac cgacgattgc gtcggcgacg gaatcgaaat gattgttcgt cacttaaagt
      181 ctacccaagt aatcctgctc gaaaacttgc ggtttcacga aggcgaagaa aaaaatgacc
      241 ctgcctttgc tcggcagctc gcgtcactcg cggaagttta cattaatgac gcatttggta
      301 gctcccatcg cgaacacgcg tcgatttacg gagttccgca gatcatgccc atcaaaggct
      361 gcggctttct tattaaaaag gaactccagt tcttaaaccg cttactcaat gctccagaac
      421 acccctacgt ggcggttctc ggcggttcta aagttagcga taaaatcaaa accatcgaaa
      481 atctattttt tgaagtcgac gttctctgtg tcggtggcgc aatggcccat gcgtttcgtt
      541 tggccgggga cccgaaacac gtactaccgc ccaacgcaaa acaaccaaag tcgcaggaca
      601 tagcctacgc ttctcaactg ctcgagaaag ctcgacgcca cgaagttaaa ttgattttgc
      661 caatagatga tcccgaaggc gttcccccag ggttcgatat cggacctcaa actatcgagt
      721 tattcactaa ggaaattcgt agggcgaaga ccgtattttg gaacggcccg gttggcatgt
      781 tcgaagacga acgctatgca aaaggtagcc tcgcggtggc ccaagctatg gccgaagtga
      841 aaggtttaaa agtggtcggt ggcggagata ccgtgtccgc tctaaacttg gctggtgtgg
      901 ccgatcaaat gacccacgta tcgacgggcg gcggtgctac cctggaattt ctagaaggaa
      961 acggattgcc gggcatcacg attctcgaca caatcggcaa aaagagcgat gcccgcatca
     1021 gtttagtacc cgaatgaaat cgcgaaaacc catattggct ggtaactgga aaatgaacct
     1081 cggcccggcc gaggcagggc tctactttca gcagctggaa acacttttgt cgaatgatcc
     1141 cactcgctca ctagacagcg aacgagtgat ctttccacca gcttataacc tgggtagtga
     1201 gatccaatgg gtcgcgggta aatgccaggt acaacttgga gctcagaaca tccactggga
     1261 atctaccggc gcctttactg cagaactgtc cgccaaagcc ttaaaagatc tcggcctgaa
     1321 ctgggcgctg gtgggccatt cggagcgccg ccagctgttc ggggagacca accagactgc
     1381 tggcaagcgc cttcttaaag gactaagtag cgggctgcgg gtgttgtttt gtatgggcga
     1441 aacattgggc gagcgtgaat caggtcgcat ggaaactgtc tgggccgagc aactcaccag
     1501 ctacgtcgaa gccttggcca aacacgtggc cagtgaccaa gcaaacgact tttctagtcg
     1561 ccatgctcca gtcgcctatg agcctgtctg ggcaattgga acgggcaaaa ctgcgacaac
     1621 tgagcaggcc gaagaagccc accgctggat tcgcaactgg ctgtctgaaa agcttggggc
     1681 cacctttgct gaagccttgc cggtcctcta cggtggcagt gtgaccccag acaacgcggc
     1741 agcgctactc aaacaaccaa atattgacgg tgttttggta gggggggcca gcttgaaacc
     1801 tgaaagtttt gcaaaaatcc tgctcgcctg atacatcctc actcatcatt tattttcaat
     1861 ggagcacact tcgtgggtac gtttctcatc atttttcacg tcatcacctg tatcgtttta
     1921 gtattcgttg ttctcattca agcggggaaa gaaggcgggc tcggcgccat gtcaggcggt
     1981 agccaaaccg tattcggaag ttcaggtgga gctaactttt ttacaaagtt tactgcggta
     2041 accgcagctc tgttcatggc tacttcgatt acactcaccg ttattaaatc gggtaaaaaa
     2101 acctctgtgt ttgacggctc agaatctagt gcccctattg agacgactac aagcccagct
     2161 actaatgctc ccgctggcgc tgcgcctagt gcgcccgctg gggaaacaac caagtaataa
     2221 acatgcttca actgcagtac cattcactcg tcgaacttaa ttttccgaac cgcgagtgga
     2281 tgtggctgcg aaagcctgtg ctgccttccg agcaaactac cggtgcgccg gatttttatt
     2341 tactcggcgt aaaatgtgac caacgttcct ttgtctggac tcgaaacgga gccgctctta
     2401 ctgacaccaa ccagggcgct tcgattactg tgccggtttt attttttaag ccggtagacg
     2461 acgaactcac tgcctacctg acctatgtaa aaaccgcatt ccaggcggtt cagccgctcg
     2521 aagtcgaatg ggtccgagtg ctaaaaacgc tcaaagagca atttcaggtc gacttagcga
     2581 aaggcattcc agcattgctt gcagcgacgc ttgccaacgc cggattggaa gctcatttgg
     2641 gagtcctggc caatcgcgcg aaatatactt tcttaacgag tcttgaactt gagttcttgt
     2701 acgaaaaaaa atgggacccc agtgtattcg aactattcga aagtatttct gttgagcttc
     2761 gtgaagctgc cctaaaggct cttcaaacct ggtctatttc tgcggcgaat gcgaaagatc
     2821 tgttgcaatc gctgttattt ctatcgcgta aaactagtgc agaaactgcg attaaaattc
     2881 tttcgtccac ctataaaacc ggagacgact tgagaattgc gatttttcat tcagcacagc
     2941 ccgaacttgc acgcctttcg cgcgaacgaa tcgagcgttt gcgagctctg agcgtgccac
     3001 cacgtacttc ggtgtttggt gacccgagtt tcgagagcga tcaacttaaa atttcgcaca
     3061 acccaaaaac catcggcgac tttgaagcat ttaagcaatg ggtttcagac cctaccacga
     3121 ctgagcgtct gcgacagctg ttggagattt atcagtaagc catgattgca aaatttgaac
     3181 gcattacagc gtcttccgaa gacatactca ctactccagc cgctcggcgc attttgaaaa
     3241 atttgcggcg ttattcaatg cttccggaca attttgaaag tcccgaaatt atttcagccg
     3301 atcgtctaag ccaagaactg gcgtttcgcg cgacctcgca acccgcgccc tacaataccg
     3361 cggtcgcact tggtaagaaa gtgctacacc ttaccactca gcccggcact tcagttaagc
     3421 agtgccctgg aaccaacaac atgttgtgct gccactacca catcatcaac atggtctcga
     3481 actgtccgtt cgactgttcc tattgttatt tgcaaactta tttgaatcag acgctcacca
     3541 ctttcttcgt aaatgaggat gatatcttcg aacaggtacg taacatctgc aaacgcccac
     3601 cggtttcgct attacgcatt ggcaccggcg agttgtccga cagtttggcg ctcgaccatt
     3661 taactgaatt ctccggacga ctggccaaca ttatgggcga atatcaaaac gtgcgtttgg
     3721 aactcaaaac caagtcaaag aatgttgaac acctgttgag cattccgaaa aaagaccatg
     3781 taatcatttc ttggagcatg aaccccccaa taatcgtaga gcgcgaagag cacggcacag
     3841 ctcgatttga tgaacgcatt cgcgctgctg caatggcggc aggagccggt tttcgagtcg
     3901 ggtttcattt cgatccactc atttacttcg atggctggaa agatcactac tccgcagcct
     3961 tagaaaaact cctggctgaa gttccccatc gttctattga ttggatcagc ctcggagcgt
     4021 tacgctttca accccaaatg cgcgcgatcg ccttaaaacg ccgccctgag actcagattt
     4081 ttcaggaaga atcggtgacc ggcgctgacg gcaaagtgcg ctatctcaaa gaaatccgta
     4141 gcgagatgtt cggctttata aataaaaaaa ttaaagatct tgctccaagt atttacacct
     4201 atttgtgtat ggaaactaaa cccgtttggg aaaaggcctt aggacgcttg cccgaccgcg
     4261 gtttttaaca attcttattt ttttatccca aatcgtgccg atacaaatgc cgcaatcata
     4321 ataagcgcag cgccggtcca ctgcaggccg ttcattcgtt cgttgaatat gaaataacca
     4381 aaaaccgcgg caaagggcga ttcgagcaaa aataacatgc tcgcagtggc agatggcaac
     4441 acacgttggg ctctcatttg aattagaaac gcaatcatcg tggaacccac caccaaaaaa
     4501 gccaaccctg cccaagacaa gctatgtgcg gcgaatggag cgtgggttag gttttttgag
     4561 tccatcgcga ttcccactgc gaagggaatt atacccaccc aaaaagactg gtaaaaatta
     4621 aacaaaaatg gcgattgaaa ctttgtactc gcacgttcga ccaatacgat ttgcactgcg
     4681 gccgctaagg cgcacaggag agtcaaaaaa tcgcctatat taaggttgtg tagattccag
     4741 gcctgacaaa gtagaccggc cccaccaagg gccaggagta ttgcgccgat atgaggtaat
     4801 ttggtcttac gtgaaaaaaa aactgcttcg aggaccggga cgaacactac gtacagcacc
     4861 gttataaagc cactattggt aacagtggtg tagcgcagac ctacggtctg taacactaag
     4921 gtggcactca aaaaaagccc gggcaaaaat gccaaaaaaa actcgtcgag cgaaagcgat
     4981 ttacgccaac gtggaaccga aacggcgagt ataaaaagta gtagcgcagc aagcaaaaat
     5041 cgtaggcccg ttaaaaaaaa agcgtcgaag ctgtccatcg cccaacgggt cgctatgaaa
     5101 ccaaaacccc ataaagcgga acaaaccaca acttctgccg ctgcacgctt ctgagtgaac
     5161 cctacattcg ttattccgcc cggcgatgac atggattggt attgtagtcg cgaaaccacg
     5221 gatccccaag ccaatacatg ggccgcggtc gaacttttac tgcctagcgg gatgtctata
     5281 atctagacac ctgactcgaa attgcgagag ctcgcctcat tgcgcatgtt tacactcgga
     5341 gtccacccct ggcatctgca ttgcacactc ttagctcgag agactaaatg cagaggagcc
     5401 cgcatatgac tatcttcacc cgttcgtttg cgcgtatgac atcgatgact cttgttttaa
     5461 ctgccatttt ggccctgact ggttcacaac cggcccatgc cgaaaagaaa cgcaaaaaaa
     5521 aggatcgcaa ggctccagtc gtagtcgttg aagagaaagt gagagtt
//
LOCUS       DAOMXO010000059         5564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7732_length_5564_cov_4.855872, whole genome shotgun sequence.
ACCESSION   DAOMXO010000059 DAOMXO010000000
VERSION     DAOMXO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7732_length_5564_cov_4.855872"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="PLH57_02155"
     CDS             <1..1254
                     /locus_tag="PLH57_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK01442.1"
                     /translation="AIKFSTKGDINVRVFEKPTSENGVMIRIEVEDHGIGIPPQALGR
                     MFQAFSQADASTTRRFGGTGLGLSICKRLVQMMGGQIDVISTEGRGSTFWFEVPLRIG
                     SKIKKEKKKRSVEKLTAGKKPYRVLVAEDNPINQKVAVLFLNKLGIHADTVANGNEAI
                     DALRNVPYDLVLMDCQMPELDGYEATRIIRSSTTMAFREIPIIAMTANAVSGDKERCL
                     AVGMNDYVGKPVGLEELSKALKPWLKKGRKKNLIAKKANTIQGENVTVSDAVTTEPVA
                     SVMKEQVAPPPPVSAEVVPLRSSSGSIDWSVIEDLRKLDEGTNSDIAAELSELFAKTV
                     PEKLKKMGECLSRGDRETLRREAHSLKSSGGNIGARKFSKLCEELEHTAEKATVPELS
                     GLFKAISDEFSVVSRELSVLSKKAA"
     gene            1263..1964
                     /locus_tag="PLH57_02160"
     CDS             1263..1964
                     /locus_tag="PLH57_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK01443.1"
                     /translation="MASLKEKHILIIDDAADVRLLARKVFETDGAIVLDADGCDSGLK
                     IAREKNPQLIILDLEMPGRSGFDFLTDRKKDPVLLEIPVLILSGKNDRQSISKAIALG
                     ASDYMLKPFRAPLLLQKTRKTLRLVSFLVKKLKPDAHIPATLSLNGDIVGIHEGGCQL
                     EASVKLGSGDRVKLVGRFIEDLGLHDVPTTVMPIPPTYANQGRYLNHIGFVGMNEERA
                     KKVRKLLKDVVKVKK"
     gene            1961..2653
                     /locus_tag="PLH57_02165"
     CDS             1961..2653
                     /locus_tag="PLH57_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK01444.1"
                     /translation="MRLKPTVLIIEDDEGFRKIMELVLARFGVNTKAVADPEEFLKAA
                     DSLNPNLYLIDLNLDGSSGFDLIKTLRERKKLTGSIVVVSGSKESEAISHALELGAND
                     YILKPLDRVLLATKLSQFIQTDEIKEHSAKQTEPTDGRVEVRLSFDGQITSVDEIGIE
                     FTSATLSPKGTVLKLNSDFFKLAGLPVTECLVTVASTWLVPDTGRYGAYAEFDGVSAE
                     FQQALRNWLSRP"
     gene            2711..3067
                     /locus_tag="PLH57_02170"
     CDS             2711..3067
                     /locus_tag="PLH57_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HRK01445.1"
                     /translation="MSQCPKCNSENTYHDGTLMICPECSYEWNPATVAAAGTGANEAD
                     DTSVRDSNGNILNNGDAVTVIKDLKIKGASSGIKSGTKIKGIRLLDDAVDGHNIACKI
                     DGIGSINLKSEFVRKA"
     gene            3316..>5564
                     /locus_tag="PLH57_02175"
     CDS             3316..>5564
                     /locus_tag="PLH57_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRK01446.1"
                     /translation="MYSSATDPGISVFCCTGCETVYQLLQSTGLTAYYDLRDRAGGET
                     KRVSETIPDLRYLDDPTFREKFEKQLDGLKRPEMDFFVEGVHCAACIWLIEKLPDLVP
                     GVESCRLDFGRSVATIRLKDRGLFSEAAQKLFWLGYRPHAIADDNEAAELKKKENRAW
                     LTKIGVAGACTGNIMLLAAALYSGVEEPYANIFRWLSGLLFAPVLFYSASSFFWNSVS
                     ALRSRSLSIDVPVFFAIVFGSAVSVWNLQTAGAHIYFDSLAMLIFLLLASRYTLRRIQ
                     QKNLGGSHLYHFIAPSRAKRFNAANGECSFVNADVLVAGDILIVEDGEVFPVDGRVIE
                     GASHVNLALLTGESNLQKIGPGDSVFAGTVNQGTALKVHTVKVGKQTRFGQILAQIEA
                     GEMTKTPIVMAADRVAKWFLAAVCLIAVGVFWLTPGFDAALSRSLALIIVTCPCALAL
                     ATPLAMSLALKNASKNGVLIHNAEVIERLSKVRNVLFDKTGTITQGKYQVLDWATDWG
                     AFASVASVVRALEQKSKHPVALALVEYLDSRSPAGIDGSATIALVEQFEERLGIGVSG
                     YVGDQYCEVRALPEQAEQVEASLQTTKQPVTRVGVFMNKKLVARIALGDGVRPGAADA
                     IRALQSTGHRAFLVSGDSVETCTVVGRSVGLPQGQIFGRLSPEQKADLAGKLNPCALV
                     GDGANDALGLKVASVGIAVHGAVDISLRVAGVYVSKPGLDYVSGLFRLAHGTMVLIRR
                     NFAFSLAYNV"
BASE COUNT         1454 a         1234 c         1481 g         1395 t
ORIGIN      
        1 gcgataaagt tttctaccaa aggcgacatt aacgtgcgtg tttttgaaaa gccaacttca
       61 gaaaacggtg tgatgatccg cattgaggtt gaggatcacg gcattggtat tccgccgcaa
      121 gctctgggtc gaatgttcca agctttctcc caagcagacg ccagtactac ccgccgcttt
      181 ggcggcacgg gcctgggatt gtctatttgt aaacgactcg tgcaaatgat gggcggtcaa
      241 atcgacgtta taagtacgga agggcgcggt tcgacgtttt ggttcgaggt gccactgcga
      301 atcggctcaa aaattaagaa ggaaaagaaa aaaagaagtg tcgaaaaact tacggccggt
      361 aaaaaacctt atcgggtgct cgtcgcagaa gataatccaa tcaatcaaaa agtcgcagtt
      421 ctgtttctca acaaactcgg aattcatgcg gacactgtag caaacggcaa cgaagctatc
      481 gatgctctac gtaacgtgcc ctacgacctc gttttgatgg actgtcaaat gccagaactc
      541 gatggctatg aggccactcg tattatacgt tcaagtacca ctatggcgtt tcgcgaaatt
      601 cccattatag cgatgacggc caacgcagtt agtggcgata aagaaagatg tctggccgtc
      661 ggtatgaatg attatgttgg aaaacccgtg ggtcttgaag aactgtcgaa ggcgctgaag
      721 ccttggttaa aaaagggacg aaagaaaaac ctcatcgcga aaaaggccaa taccattcag
      781 ggtgaaaacg ttacggtgtc agatgcggtt acgactgaac cggtggcgag cgtgatgaag
      841 gaacaagtag cccctccgcc gcccgtgagt gccgaggtgg tgcccctgcg ttcctcgtcg
      901 gggtccatcg attggagtgt catcgaagat ctgagaaaac tagatgaagg cacgaacagt
      961 gatattgccg ctgaactgag cgagttattt gccaaaaccg taccagagaa attaaagaag
     1021 atgggtgagt gcctcagtcg tggggatcga gaaacgttaa ggcgagaagc tcattcgcta
     1081 aaatctagcg gcggtaatat cggtgcgcgc aagttttcca agttgtgcga agaacttgag
     1141 cacactgctg agaaggcgac ggttccagaa ttatcagggc tatttaaggc cattagcgat
     1201 gagtttagtg tcgttagtcg agaattaagt gttttaagta agaaagcagc ttaggtgttg
     1261 aaatggcatc tttaaaagaa aaacatatac tcattatcga cgacgcagcg gacgtgcgtt
     1321 tacttgctcg caaagtgttt gaaactgacg gggcaatagt gctggatgcc gacggttgtg
     1381 actcgggact taagattgct cgtgaaaaaa atccgcaact gatcattctt gatttggaaa
     1441 tgccggggcg aagtggcttt gactttttga ctgaccgaaa aaaggacccg gttctgttag
     1501 aaattcccgt acttatactt agtggtaaga acgatcggca gtccattagt aaggccatcg
     1561 cgctaggggc cagcgattat atgttgaagc cttttcgggc gccacttctg ttgcaaaaaa
     1621 cccgaaaaac acttcgttta gtatcttttc tagtaaaaaa gttaaaaccc gatgcccata
     1681 ttcccgcaac cctttcactt aatggcgaca tagtcggcat tcacgaaggt gggtgtcagt
     1741 tagaggcctc agtaaagctt ggatcgggtg atcgcgttaa attagttggt cgatttatcg
     1801 aagatctcgg cttgcacgac gtaccaacaa ctgtaatgcc gattcctccg acctacgcga
     1861 atcaggggcg atatttgaat cacatcggat tcgtaggaat gaacgaagaa cgggcaaaaa
     1921 aggtacgaaa actcttgaaa gacgtagtga aggttaaaaa atgagactta aacctacggt
     1981 acttataatc gaagatgacg aaggttttcg aaaaatcatg gagttagtcc ttgcgcgatt
     2041 cggtgtgaat accaaggcgg tggccgatcc cgaagaattt ttaaaagcag ccgattcctt
     2101 gaatccaaat ctgtacttga ttgacttgaa tttagacggg tccagtggat tcgatttaat
     2161 aaaaacgctc cgcgagcgga agaagctgac cggctctata gtcgtagtgt caggctccaa
     2221 agaaagtgaa gcaatatctc acgccctgga gcttggagct aacgattata tactgaagcc
     2281 gcttgaccgc gttctgcttg caacaaaact ttcacagttt attcagaccg acgaaataaa
     2341 agaacatagc gccaaacaaa cggagcctac cgacggtaga gttgaggtgc gtttgagttt
     2401 tgatggccaa attacctcag tggacgaaat cgggattgaa ttcactagcg cgacactttc
     2461 acctaaaggg acggttctta agctaaatag tgatttcttt aaattggcag ggctgccggt
     2521 taccgaatgt ttagtcacag tggcgtcgac ttggttggtt cccgatactg ggcgctacgg
     2581 cgcatacgca gagtttgatg gcgtcagtgc tgagtttcaa caggcgctgc gtaactggct
     2641 gtctcggcca taatcttcgt ggcccagtct gcggtttatc tacgctgcct tctgtggtac
     2701 ggtcaaaccc atgtcacaat gtccgaagtg caattcagaa aacacctatc acgacgggac
     2761 gctcatgatt tgtcccgaat gctcctacga gtggaatcca gcgacagtgg ccgcggcggg
     2821 gactggcgcc aatgaagccg acgacacctc ggtgcgagat tccaacggaa acattctaaa
     2881 caacggcgat gccgtgaccg tgataaagga tctaaaaata aagggtgcgt cgtccggtat
     2941 taaatccggc accaagatca agggcataag actgttggac gatgcagtag atggtcacaa
     3001 catcgcctgc aaaattgacg gtattggttc gataaatctt aagtccgagt tcgtaagaaa
     3061 agcttaagta aattagtttg tcgcactcag ttcgctacaa aaagaagcac tgagacaaaa
     3121 tgtctcgcgg cgtgctgttt caccccggaa aaaaatcaaa atttaacttt attcccaaaa
     3181 aagcgctgtc ctcggcggca cgatggttgc atattagaaa gaaaaaaggt gagcatcgtg
     3241 aatttccagt tatcgttaaa tcgatcaaat cacgttgatc gcgagcctgt cgcttgcgct
     3301 cactgtagga aaccggtgta ttcttcagcg accgatcccg ggatttcggt gttttgttgc
     3361 accggttgcg aaacagtcta ccaattgctg cagtctacgg gtctcaccgc ttactatgat
     3421 ctgcgagaca gggccggtgg cgaaaccaag cgcgtctctg aaaccattcc tgatctacgg
     3481 tacctcgacg accctacgtt tagagagaaa tttgaaaaac agctcgacgg tttgaagcgc
     3541 ccggaaatgg atttctttgt tgaaggcgta cattgtgcgg cctgcatttg gttgatcgaa
     3601 aaactaccag accttgtgcc cggagtggaa tcttgtcgcc ttgattttgg tcggtctgtc
     3661 gcaactattc gccttaaaga ccgaggctta tttagtgaag ccgctcagaa gttattctgg
     3721 ttagggtatc gaccccacgc cattgccgat gataacgaag ccgcggagct gaaaaaaaag
     3781 gaaaatcgcg cctggttaac taaaattgga gtcgctggcg cgtgtaccgg taacatcatg
     3841 cttctggccg ccgcgcttta ttcaggggta gaagaaccct acgctaatat ctttcgatgg
     3901 ctttctggtt tgttgttcgc tcctgttttg ttttattcag ctagttcctt tttttggaat
     3961 tcggtgtcgg cactgcggtc tcgcagcctc tcgattgacg taccagtatt ttttgccatt
     4021 gtttttgggt cagccgtgag tgtgtggaac ctacaaacgg cgggcgctca tatttatttt
     4081 gattcgcttg cgatgttaat ttttctattg ctcgcgagtc gttacacgct ccgtagaatt
     4141 cagcagaaaa acctgggggg ctcacaccta taccatttta tagcgccgtc gcgggccaaa
     4201 cggttcaatg ctgcaaacgg cgagtgtagt ttcgtaaatg cagacgtgct ggtcgctggc
     4261 gatattttaa tagtagaaga cggagaggtg ttccccgtcg acggtcgagt gatcgaaggg
     4321 gcgtctcatg tgaacctcgc cttgcttacc ggggaatcga atcttcaaaa gattggcccc
     4381 ggcgacagcg tttttgcggg cacagtaaac caggggactg cgttaaaagt tcatacggta
     4441 aaagtcggta aacaaacgcg atttggccaa atactagccc aaattgaagc gggcgaaatg
     4501 acaaaaacgc ccattgtgat ggctgccgac cgagtggcca aatggtttct agcggcagtg
     4561 tgtctgatag ctgtaggagt gttttggtta actccgggat ttgatgcggc acttagtcgg
     4621 tccttggcgc tgatcattgt aacatgcccc tgcgcattgg cacttgctac gccactcgca
     4681 atgagtttag cgctcaagaa cgcctcgaaa aatggcgttc ttattcataa cgcggaagta
     4741 attgaacggc tttcgaaggt gagaaatgtt ttattcgata agaccggcac tattacccaa
     4801 ggaaaatacc aggttcttga ttgggcgacc gactgggggg ctttcgcttc ggtggcgagc
     4861 gtggtgcgtg cgctcgaaca aaagtcaaaa caccccgtgg cgttggcgct agtagaatat
     4921 ctcgattccc gttcgccggc gggcattgat gggagcgcaa ctattgctct ggtcgagcaa
     4981 ttcgaagaac gtttaggaat cggtgtttcc ggttacgtag gcgatcagta ctgtgaggta
     5041 cgggcgctac cggaacaagc ggaacaagtc gaagcgagtc ttcaaactac taagcaacct
     5101 gtgactcgcg ttggtgtttt catgaataaa aaactagttg ctcgaattgc gttgggagac
     5161 ggcgttcgac ctggggccgc cgatgccatc cgcgcgcttc aatcaactgg ccaccgagca
     5221 tttctggttt cgggtgatag cgtagagacc tgcacagttg ttggacgcag cgtgggttta
     5281 cctcagggcc aaatattcgg gcgtttatcc cccgaacaga aagcagatct cgcaggtaaa
     5341 ttgaatccgt gtgctttggt cggcgacgga gctaacgatg ccttgggcct aaaggtcgcc
     5401 agcgttggca ttgcggttca cggggccgtc gatattagcc tgcgagttgc gggagtctat
     5461 gtgtcgaaac ctggattgga ttacgtttcg ggcttgtttc ggctagctca tggcacgatg
     5521 gttttgatac gaagaaattt cgccttttcc ctggcttaca acgt
//
LOCUS       DAOMXO010000060         5525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7800_length_5525_cov_4.533090, whole genome shotgun sequence.
ACCESSION   DAOMXO010000060 DAOMXO010000000
VERSION     DAOMXO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7800_length_5525_cov_4.533090"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1001
                     /locus_tag="PLH57_02180"
     CDS             150..1001
                     /locus_tag="PLH57_02180"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008913628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRK01447.1"
                     /translation="MQNRHLEAARFIEHHSSVKPRLGIVLGSGLGRFADLVRNAVQIP
                     YTDIPHFTGTTVEGHEGRLVLGTIAPNPTVPAVPVAVLQGRLHIYEGHTMDRVVFPVR
                     TLCLLGVDTIVLTNSAGAVNPAFRESDIMMITDHLNLMGDNPLIGPHLKDFGPRFPDL
                     TEAYHPECQQALRDAARNESITLHEGVYAALTGPTYETPAEVRMLRVLGADAVGMSTV
                     PEAIAANHLGVRVAALSVLTNLAAGISPHRLSHNEVMKNADQACSKLVKLLSRAIPNL
                     PVKRNAQ"
     gene            998..1453
                     /gene="cdd"
                     /locus_tag="PLH57_02185"
     CDS             998..1453
                     /gene="cdd"
                     /locus_tag="PLH57_02185"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRK01448.1"
                     /translation="MTTKKMKKKRSSNTDIRIQDVQEILFDAAKNVMRQAYAPYSKFR
                     VGAALIDETGTIHVGTNVENASYGGTICAERAAICSMIAGGGRKLRAIAIVTEKGTPP
                     CGLCRQILSEFAGPEVPVIIAGKKGVLDLYSVGELLPESFGPKNLLKHS"
     gene            1489..1995
                     /gene="rplU"
                     /locus_tag="PLH57_02190"
     CDS             1489..1995
                     /gene="rplU"
                     /locus_tag="PLH57_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRK01449.1"
                     /translation="MYAIIETGGKQYRVAPGEVHTFEHINGNVGDSITFDKVLLVGTQ
                     GGDTSVGTPYVNQATAIGEIVQQTRGEKLLTIKYRRRKGYRRTIGHRQELTRVLVTKL
                     ANGAGSETQYDSAKRNELLIKASVPFAAAKTEKPRKAKLAAAPTSEKAAKTATKKAPA
                     KKASKKEA"
     gene            2013..2273
                     /gene="rpmA"
                     /locus_tag="PLH57_02195"
     CDS             2013..2273
                     /gene="rpmA"
                     /locus_tag="PLH57_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRK01450.1"
                     /translation="MAHKKAGGSSKNGRDSNPKMRGVKRYGGQVVNPGEVLVRQVGTK
                     FHPGRNVRLASDYTLYSAIGGVVKFEHHDKKRLQVSVYPLAS"
     gene            2356..3399
                     /gene="obgE"
                     /locus_tag="PLH57_02200"
     CDS             2356..3399
                     /gene="obgE"
                     /locus_tag="PLH57_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRK01451.1"
                     /translation="MAKFIDEASITIQSGNGGPGCVAFHREKYVPKGGPSGGDGGKGG
                     DVWIETNRNLATLLDYRYRLKYEAKNGKPGQGGMKTGSDGEDVTLKIPVGTIVRDLDT
                     GEVLEDFTEHGTRYLLCKGGRGGKGNSHFATSTHQAPRFAQPGEEGVVRKIKLELKLL
                     ADVGIIGYPNAGKSTLISKISAARPKIADYPFTTLVPNLGVVRVGKEIGSPTFVVADI
                     PGLIEGASLGRGLGTKFLKHIERTKFLLHVIDGAKLLKEFGTDDPKAFAQAVVRDKEI
                     IDAELYTFSPELGEKAQMLVLNKCDLFTDEFLKTVQKHLTKLKFTDILMISGVAHQNL
                     DDLVERLARRLGL"
     gene            3396..4130
                     /locus_tag="PLH57_02205"
     CDS             3396..4130
                     /locus_tag="PLH57_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HRK01452.1"
                     /translation="MNPIESPRAVAIFGGRFDPVHSGHVTLAEICRKHLNFATLEIVP
                     TKNAPHKVAAVATDDERVELLKLAFKDFSGARINDWELKKDHTCYTTDLLDHYAAQGA
                     EQPFFILGEDSLYDLPKWYQFPKLLFKARWVAVRRGTFPNRIDRQAHLLESLEKTGCQ
                     INCQETPVGVRWTISNLPNQPVNSTTIDLFSAEIPDIRSQDIRKGLYDHGGHWLDSHP
                     VVPPAVAQYLKTHHLYVKKQGLTNGI"
     gene            4120..4497
                     /gene="rsfS"
                     /locus_tag="PLH57_02210"
     CDS             4120..4497
                     /gene="rsfS"
                     /locus_tag="PLH57_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010619956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRK01453.1"
                     /translation="MESRELALACARAAIDKKAEHSLVFDLNGVADFTDYFLITSGQS
                     ERQVQAIAQSILDHTRSFGDTRPRVEGLDSGRWVLIDFGDVVVHVFHHYIREFYDLET
                     LWSDARRVNIPKEFYTEPAHAPS"
     gene            4506..4958
                     /locus_tag="PLH57_02215"
     CDS             4506..4958
                     /locus_tag="PLH57_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRK01454.1"
                     /translation="MRIRFLVLGKFKNKALEELFNYYIKLATQFAKVESIALGVTSRT
                     EQDETVSRWLEKNAHRSYVTVLDERGKEFDSQGFAKKMEQIRGGSHSDWTIICGGAWG
                     FSDSLRSKAHLEWALAKMTLPHELALVVSAEQVFRGLSILANHPYHHQ"
     gene            4970..5449
                     /gene="greB"
                     /locus_tag="PLH57_02220"
     CDS             4970..5449
                     /gene="greB"
                     /locus_tag="PLH57_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HRK01455.1"
                     /translation="MGSDTKNYITPGGFTKLKDELKQLLDVERPTVVRTVSWAASNGD
                     RSENGDYIYGKRRLREIDRRIRFLSKRIESAEVIDPAKQSGERVVFGALVEVETSDGT
                     VKRYQIVGIDESEPAVGRLSWISPIAKALLGKKVGDLVLYSTPKGEEELAILKISFS"
BASE COUNT         1496 a         1467 c         1304 g         1258 t
ORIGIN      
        1 acgcctaaaa ctctatccaa cttcaaagag caatttcaaa acgctttcga aatcacaact
       61 cggcacgtga cggctccgaa actaatcaaa aaagtcattg attatcaccg accttagcag
      121 gacgatctta ttcggccctg aaaagatcta tgcaaaaccg acacttagaa gcagcacgct
      181 tcatcgaaca tcacagttcc gtcaaacccc gtctcggaat cgttctgggc tcggggcttg
      241 ggcgttttgc cgaccttgta cgcaatgcag ttcaaatccc ctacaccgac attccccact
      301 ttacgggcac gaccgttgag ggccacgagg gccggttggt tttaggcacc attgctccta
      361 accctacagt cccagcggtg ccagtggccg ttcttcaagg ccgcttgcac atctacgagg
      421 gccacaccat ggatcgcgtg gtgtttccgg ttcgaacact ttgtctattg ggcgtcgaca
      481 ccatcgttct gacgaactcg gctggtgccg taaatcctgc atttcgcgaa tctgacatca
      541 tgatgatcac cgatcatttg aacctcatgg gagataaccc gctgattggc ccccatctca
      601 aagacttcgg accacgtttt ccggacctca ccgaggccta ccatccggag tgtcagcagg
      661 cgctccgcga cgccgcccga aacgagtcca taactctcca cgaaggggtt tacgcggctc
      721 tcactggccc tacctatgaa actccggcgg aagtacgaat gctccgtgtc cttggagctg
      781 acgccgtcgg catgtcgacc gtccccgagg cgattgccgc caatcactta ggtgttcggg
      841 ttgccgccct ttcggttctt acaaaccttg cggctggaat ctcaccccat cgactttccc
      901 ataacgaagt catgaaaaac gcggatcagg cctgttccaa actcgttaag ctcctaagcc
      961 gagccatacc gaacctaccc gtaaagagga acgcccaatg acaaccaaaa aaatgaagaa
     1021 gaaaagaagc tccaacaccg acatccgaat tcaagacgtt caggaaatcc tcttcgacgc
     1081 cgccaagaac gtcatgcgcc aggcctatgc cccttactct aagtttcgcg ttggggccgc
     1141 tctcatagac gaaactggca ccatccacgt cggcacaaat gtcgaaaacg catcgtacgg
     1201 tggcacaatc tgcgcggaac gcgcggcaat atgctcgatg atcgcgggtg gagggcgtaa
     1261 gcttcgcgcc attgcgattg tcaccgaaaa gggcaccccc ccctgtgggc tgtgtcgcca
     1321 gattctgtcg gaattcgcgg gtcccgaagt ccccgtcatc atcgcaggaa aaaaaggggt
     1381 cctagacctt tattcggtcg gcgaactact gcccgaatcc tttggtccga aaaacctttt
     1441 aaaacattct tgacctacag acacggaaca cgttaagaaa ccccacctat gtacgcaatc
     1501 attgaaactg gtggaaaaca atatcgtgtc gctcctggtg aagttcacac ttttgagcac
     1561 attaacggca atgttggtga ttcgatcacc ttcgacaagg ttctcttggt aggaactcaa
     1621 ggcggtgaca cttcagtggg cactccttac gtaaaccaag caaccgcgat cggagagatc
     1681 gtgcaacaaa ctcgtggcga aaaacttctc acgatcaagt atcgtcgtcg gaaaggctat
     1741 cgtcgcacga ttggtcaccg ccaagaactg actcgcgtac tcgttacgaa attggcaaac
     1801 ggcgccggtt ccgaaactca gtacgattct gcaaagcgca acgaactcct cattaaagct
     1861 agcgttcctt ttgctgctgc taaaactgaa aagccacgca aagcaaaact tgctgctgct
     1921 ccaacgagtg aaaaagctgc gaaaacagca actaaaaaag ctccggctaa aaaagcctca
     1981 aaaaaagaag cttaaaaaaa ggatagatcg tcatggcaca taaaaaagcg ggtggtagtt
     2041 ccaaaaacgg tcgcgactcc aaccccaaaa tgcgcggcgt aaagcgctac ggcggccaag
     2101 tggtaaaccc aggtgaagtt ctagtacgcc aagttggtac caagtttcac cctggtcgca
     2161 acgttcgcct agctagcgat tacaccttgt attcggctat tggtggtgtt gttaagtttg
     2221 aacatcacga taagaaaaga ctgcaagtct cggtttaccc gctggcttcc taagtcttag
     2281 cctttttact ttttaaaaaa aattaaccca ggtcgcgcag aaatagtgta taagcgcgcc
     2341 tgggtttttt gttttatggc aaagtttatt gacgaagcaa gtatcactat tcagtcgggc
     2401 aatggcggcc ccggctgtgt ggcgtttcac cgcgagaagt acgtgcctaa aggtgggcct
     2461 tccggcggcg acggcggcaa aggcggcgac gtctggattg aaactaaccg caatctagct
     2521 acccttttag actatcggta ccgcttaaag tacgaagcaa aaaacggcaa acccggccaa
     2581 ggcggaatga aaaccggctc cgatggcgaa gacgtcactc tcaaaattcc ggttggcact
     2641 atcgtgcgag atctggacac cggcgaagta ctcgaagatt tcacagaaca cggcactcgc
     2701 tatctcctgt gcaaaggggg acgcggcgga aaaggcaatt ctcattttgc cacttcaact
     2761 catcaagctc cacgttttgc acagcccggc gaagaaggcg tggtccgaaa aataaaactc
     2821 gaactaaaac tactcgctga cgttggtatt attggatacc cgaatgctgg caagtcgacc
     2881 ctgatttcca aaatttctgc agcgaggcca aaaattgcag actacccgtt taccacttta
     2941 gtgcccaacc ttggagttgt gcgtgtgggt aaagaaattg gctcccctac ttttgttgta
     3001 gctgatattc ccggactaat tgaaggagcc agcctcggac gcggtcttgg cactaagttt
     3061 ctcaaacaca tcgaacgtac gaagtttctc ctccacgtca ttgacggtgc caaactcctt
     3121 aaagaatttg gaacagacga tccaaaggct tttgctcaag cggtagttcg tgacaaagaa
     3181 attatcgacg ctgaacttta tacgttttcg ccggaattag gcgaaaaggc ccaaatgctc
     3241 gtgctaaata agtgtgattt atttaccgac gagtttctta aaactgttca aaaacacctc
     3301 actaaattaa agttcaccga catactgatg atttcgggag tagcacacca aaatttagat
     3361 gaccttgtcg agcgcctcgc ccgccgcctg gggttatgaa cccaatcgaa tcgccccgcg
     3421 cagtagcgat tttcggagga cgcttcgatc cagttcattc ggggcacgtg acacttgcgg
     3481 agatttgtcg caaacattta aatttcgcca ctctcgaaat agtgccgacc aaaaatgctc
     3541 cgcacaaagt cgcagcagtc gcgaccgatg acgaacgtgt agaattattg aagcttgcct
     3601 ttaaagattt ttccggggct cggatcaatg actgggaact taaaaaagat cacacttgtt
     3661 acacaaccga cttgcttgat cactacgcgg cccaaggcgc cgaacaacct ttttttattc
     3721 taggcgaaga ctcactctac gacttaccga agtggtacca gttccccaag ttactcttca
     3781 aagctcgctg ggtggccgtg cgtcgtggta cctttcccaa tagaatcgac cgtcaagccc
     3841 accttctcga gagtcttgaa aagactggat gtcaaattaa ctgccaggaa accccagtcg
     3901 gagttcgttg gaccatcagc aatctgccga accaaccagt aaattcaacc acgatcgatc
     3961 tatttagcgc cgagattccc gacattcgtt cgcaagatat aagaaagggg ctttatgatc
     4021 atggggggca ttggcttgat tctcacccag tggttccccc cgcggttgcc cagtatttga
     4081 aaacccatca cctgtatgtt aaaaaacaag gactaaccaa tggaatctag agaactcgca
     4141 ctcgcctgtg cccgcgcggc gatcgataaa aaagcggagc acagtctggt gttcgaccta
     4201 aacggcgtag ccgacttcac ggactatttt ttaatcacct cgggtcagtc cgaacggcag
     4261 gtccaagcca ttgcacagtc gatactagac cacacaagat cgttcgggga caccagaccc
     4321 cgtgtagaag gattagattc tggacgttgg gtgttgattg attttggcga cgtagttgtt
     4381 cacgtttttc accactacat ccgtgagttt tacgacttag aaacgctctg gagtgatgca
     4441 cgacgcgtta acatacctaa ggaattttac accgaacccg cgcacgcgcc gtcctgatta
     4501 gcgacatgcg tattcgtttc ttggttctgg gaaaatttaa aaataaagcc ctcgaagaac
     4561 tcttcaatta ctatataaaa ctcgctacac aattcgccaa agtcgaatcc atcgcattag
     4621 gtgttacttc tcgcacagaa caagacgaaa cagtttcgcg gtggctcgaa aaaaatgccc
     4681 accgttccta cgttaccgtg ctcgacgaac gcggcaaaga attcgatagc caagggtttg
     4741 cgaaaaagat ggagcaaatc cgcggtggct cccacagcga ttggaccatt atttgtggtg
     4801 gggcctgggg tttttcggac tcacttagat ctaaggccca cttggaatgg gcactggcga
     4861 aaatgactct cccccatgaa ttagcactgg tggtaagcgc tgaacaagtt tttcgcggcc
     4921 tttccatttt ggcgaaccac ccgtaccatc accagtgaga aactaaataa tgggatccga
     4981 cactaaaaac tacatcaccc ccggtgggtt caccaaactc aaagacgaac tcaaacaatt
     5041 actcgacgtc gaacgcccaa cagtagtgcg taccgtcagt tgggccgcgt ccaacggaga
     5101 tcgctccgaa aatggggact acatttatgg caaacgacga ctgcgggaaa tcgatcgtcg
     5161 cattcgcttc ctaagcaaac gaattgaaag cgcggaagtt attgatcccg ccaagcaaag
     5221 cggagaacgt gtagtttttg gcgcgcttgt cgaagtagaa acaagtgatg ggactgtgaa
     5281 acgttatcaa attgtgggaa tcgatgagtc tgaaccagcg gtcggtcgat tgagctggat
     5341 ctcgccgatc gcaaaagctt tgctgggaaa aaaagtcggc gacctagtcc tgtacagtac
     5401 cccaaaaggc gaagaagagc tggcaattct aaaaattagc ttttcgtaat ttcatcacaa
     5461 ccgggctcca acatgtgcgc gtgatctgcg gcggcttttg gccagtactc tcgcgtcaca
     5521 agctc
//
LOCUS       DAOMXO010000061         5437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7965_length_5437_cov_6.372910, whole genome shotgun sequence.
ACCESSION   DAOMXO010000061 DAOMXO010000000
VERSION     DAOMXO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7965_length_5437_cov_6.372910"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLH57_02225"
     CDS             complement(<1..233)
                     /locus_tag="PLH57_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01456.1"
                     /translation="MEKSKTSQTSASPTVDFIWDTERVVHSGDGYFHEMVEDFRAAFF
                     TIDIEVYIFDPDDVGKIIVRELISAAARGVRVRV"
     gene            292..1065
                     /locus_tag="PLH57_02230"
     CDS             292..1065
                     /locus_tag="PLH57_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK01457.1"
                     /translation="MNNSRWVFITGASSGIGRACAELAFKKGYSVFATARRQTNLETL
                     KRSLKKGRKNRFEFAKLDVRSAKEVESFAKKHRELLSKTQILINNAGLARGLDSFAEP
                     LAGDWTEKVNAMIDTNVKGLLWVTRALLPHLIKNRGHIVNIGSIAGQWVYPRGNVYCA
                     SKFAVRALNEALRQDLLGTGVRVTEVAPGMVETEFSLVRLGDAQSAKNVYRGMHPLTA
                     ADIAESVVWCIGRPKHVNVQSLTLYPIDQASPTLVHRHT"
     gene            1221..>5437
                     /locus_tag="PLH57_02235"
     CDS             1221..>5437
                     /locus_tag="PLH57_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRK01458.1"
                     /translation="MLNRTMGNIELVLDALRRHVLKFAHVLSIVLLSTAIYGCNAKII
                     GEGGGGALGGGGGGSAGGSSGGGSTPVGPTVTNVTSSTANGTYSATDSVSISITFSEI
                     VNVVGTPTLVLETGATDRTVNYSSGTGTTTLVFTYTVQPGDTSADLDYVGTGSLAAGT
                     SIRNAAGTDATLTLPSPGAANSLSANKAIVIDNTGPTVTSVSSSTANGTYTTGGTVNI
                     TVTFSEAVTVTGTPTIALETGATDRTVNYSSGTGTTTLVFAYTVQAGDASGDLDYLST
                     TALSAGTSIRSAIGNDATLTLPTPGAANSLGANKAIVIDTGAPSVTSVSATTANGSYN
                     AGDTVDVTVTFSEVVTVTGTPTIVLETGATDRTVNYSSGTGSATLTFTYTVQAGDTSS
                     DLDYVGTGSLAAGTTIRDGVGNDATLTLPSPGAANSLGANKAIVIDTTAPTVSSVSST
                     TNNGTYAAGSTVDVTVTFSESVTVAGTPTIVLETGATDRTVNYSSGSGGTTLTFTYTV
                     QAGDTSSDLDYVATTSLAAGTSIRDAAGNDATLTLASPGAANSLGANKAIVIDNTGPT
                     VSSVNSPTAAGTYKAGDTIDVVVNFNETVTVVGTPTIVLETGATDRTVNYSSGSGSTA
                     LTFTYTVQAGDTSSDLDYVATTSLAAGTSIRDSVGNDATLTLASPGAANSLGANEALV
                     IDTTGPTVSSVNSPTAAGSYNAGDTIDVVVNFNENVTVVGTPTIVLETGATDRTVNYS
                     SGSGGTALTFTYTVQAGDTSSDLDYVATTSLAAGTSIRDAAGNDATLTLASPGAANSL
                     GANEALVIDTTAPTVSSVNSPTAAGSYNAGDTIDVVVNFNENVTVVGTPTIVLETGTT
                     DRTVNYSSGSGGTALTFTYTVQAGDTSSDLDYVATTSLAAGTSIRDAAGNDATLTLAS
                     PGAANSLGANEALVIDTTAPTVTGVASNSAAATYGLTDVIDVRVTFSENVTVVGTPTI
                     ILETGATDRTVNYVSGTGTTELLFNYTVQAGDSSADLDYVGTTSLTAGTSIRDAAGND
                     ADRTLASPGAAGSLGANEAIVIVTPTAVPSDFPGFQIAYGDFDRDGDIDVVIPSLSAN
                     EVIPYLNNGSGTLSAGTAVTVGTKPVAVLAADINRDGKLDLVVSNWDADTVGTLIGVG
                     DGTFGAMTSVAVGDKPAGLAALDINKDGDLDIVVANYGADNVQLLSNSGLGILTAGAT
                     NATGTGPTSVVAGDWDRDGDQDIAVTNFGSGTVTVLATALGLLGADESITVGTNPFGI
                     VVGDFNADGKPDLAVANYGDNDVSVSLNGGTSFGAKTDTAVGTGPIGIAAVRWNAGGA
                     LDLVISNSGSDNYSVLSGVGDGTFGAAANTALGSTDPSGVAIGDFNRNGEPDVVFMYS
                     TGATATALASGSGTTGDGTFTVTQTLS"
BASE COUNT         1278 a         1542 c         1369 g         1248 t
ORIGIN      
        1 acccgtactc tcactcctcg tgccgcagca gaaatcagtt ctcgcacaat aatttttcct
       61 acgtcgtctg ggtcgaaaat ataaacttcg atatcgatcg taaaaaatgc tgcacgaaag
      121 tcttcgacca tttcatggaa atagccatca cccgagtgaa cgactcgttc ggtatcccaa
      181 ataaaatcaa ctgtgggcga agcactggtt tgcgatgttt tggacttttc catgttctca
      241 atactataaa taccctcttt ctttcgccgg gggtacggat taaatagaag catgaacaat
      301 tcaagatggg tttttattac tggcgcgagc agtggtatcg ggcgcgcatg cgccgaactg
      361 gcgttcaaaa aaggttattc agtattcgct actgcgcgtc gccagacgaa cttagaaacg
      421 ctcaaacggt ctctgaaaaa aggtcgaaaa aaccgcttcg aattcgctaa actcgacgtg
      481 cgaagcgcca aggaagttga atccttcgca aaaaaacatc gtgaattact ctctaaaact
      541 cagatactta tcaacaacgc tggtctcgct cggggactcg actcctttgc cgaaccacta
      601 gccggtgact ggaccgaaaa agtaaacgcc atgatcgata ccaacgtaaa aggcttgctt
      661 tgggtcacgc gcgccctctt gccacatctc attaaaaacc gagggcacat cgttaatata
      721 ggttcgatcg cgggtcagtg ggtgtatccg cgaggcaacg tttattgtgc cagcaaattt
      781 gcggtaagag cactcaacga agctctgcga caagatctct tgggcaccgg agtaagagtg
      841 acggaagttg ctcccggcat ggttgaaacc gagttcagtc tggtgcggct gggcgatgcc
      901 caaagcgcaa agaacgtcta tcgcggcatg cacccgctta ctgccgccga tatcgcggag
      961 tcggtagttt ggtgtatcgg gcgtcccaaa cacgtcaacg tccagtcgtt gaccctttat
     1021 ccgatcgacc aagcctcgcc caccctggta caccgacaca cctagtcgac cggccgtgcc
     1081 aattgttaag aactgcatgc aattcgatca ttgatcgtcg taaatatcta attttattat
     1141 attttatatt agaccatttt tgtcgtcaga attgttttcc ggcaaaaacg acgtacacta
     1201 ttgggtatag aggggtccgt atgttgaacc gaacaatggg gaacattgaa ttagtactgg
     1261 atgcgcttag acgccatgtc ttaaagttcg cgcacgtact cagtatcgtt ctcctgagca
     1321 cggcgatcta cggatgtaat gcgaaaatca ttggtgaagg cggcggtggc gcactcggtg
     1381 gcggcggcgg tggttcagca ggcggcagca gcggtggcgg ttccacgcca gttggcccta
     1441 cagtcaccaa cgtcacttca tcgaccgcta acggaacgta ttcagcaacc gactccgtga
     1501 gcatctccat cacattttcg gaaatcgtaa acgtagtggg cacacctaca ttagtgcttg
     1561 aaactggtgc aactgaccgt accgtaaact actcttcggg aacaggcacc actactcttg
     1621 tttttaccta cactgtgcaa ccgggcgata cctcggcaga tttagactac gttggcactg
     1681 gctcacttgc agccggtaca agtattcgaa acgcagctgg aaccgatgca acgctcacgt
     1741 taccaagtcc tggtgcggcc aactcgctta gcgcaaacaa agctatcgtg atcgacaaca
     1801 cgggcccgac ggttactagt gttagttctt ctactgccaa cggcacctac accacgggcg
     1861 gaaccgtaaa catcacagtc actttttccg aggccgtaac cgtaaccggt acaccaacga
     1921 tcgcacttga aacaggtgca acagacagaa ccgtgaacta ctcctcggga actggcacca
     1981 cgacattggt gttcgcttat acggtgcaag ccggcgatgc gtcgggtgat cttgactacc
     2041 tttcaaccac tgctctgagc gccggcacaa gtattcgcag tgctattggc aacgacgcca
     2101 cactcacact gcctactcca ggcgccgcaa actcacttgg cgcgaacaaa gccattgtaa
     2161 tcgatactgg tgctccatca gtcacgagcg ttagtgcaac gactgctaac gggtcctaca
     2221 acgctggcga tacggttgac gtaaccgtaa ctttttctga ggtcgtaacg gtcactggca
     2281 ctccaacgat cgtattagaa accggagcca ctgatcgcac ggtgaattat tcttctggca
     2341 ctggttccgc tacgttgaca tttacctaca cggttcaagc cggcgatact tcttccgacc
     2401 tggactacgt aggaactggt tcgcttgcgg ctggtactac gattcgtgac ggagtcggca
     2461 acgacgcaac tctaacgctt ccttcgccag gcgcggcaaa ctctctggga gcgaataaag
     2521 ctatcgtcat cgacaccact gcaccgacgg tttctagcgt aagttctacg accaacaacg
     2581 gtacctacgc agcaggcagt acggttgatg tcaccgtaac attttctgag tcggtcactg
     2641 tggcaggaac accaactatc gttcttgaaa cgggcgccac cgaccgcacc gtaaattatt
     2701 cttcgggtag cggcggcacc actctgacgt tcacctacac tgtacaagcc ggcgatactt
     2761 cgtcagatct agattatgta gccaccactt cactcgcagc tggcactagc attcgcgatg
     2821 ccgcgggcaa cgatgcgact ctaacgcttg ccagccctgg tgcggccaac tcgttagggg
     2881 caaacaaagc aatcgtcatc gataacaccg gccctaccgt ttcgagtgtt aattctccaa
     2941 cggcagctgg cacctataag gccggcgaca ctatcgacgt agttgttaac ttcaacgaaa
     3001 cggtaacggt tgtcggtacc ccaactatcg ttttagaaac tggcgccacc gaccgcaccg
     3061 taaattattc ttcgggtagc ggaagcaccg cactcacttt tacctacaca gtgcaagccg
     3121 gtgatacttc ctcggacctt gattacgtgg ccaccacttc gctcgcagcc ggcactagca
     3181 ttcgcgactc ggtgggtaac gacgctacct taacactcgc cagccctggg gccgctaact
     3241 cgctcggtgc taacgaagca ctcgtgatcg acaccactgg tccaacagtt tcgagtgtaa
     3301 actcgccaac tgcagccggg tcgtacaacg ctggcgacac tatcgatgtc gtcgtcaact
     3361 tcaatgaaaa cgtgaccgta gttggtactc caaccatcgt attagaaact ggcgctaccg
     3421 accgcacagt gaactactcc tcgggtagcg gcggtaccgc actcactttt acctacacgg
     3481 tgcaagccgg cgatacttcg tcagaccttg attacgtggc caccacttcg ctcgcagccg
     3541 gcactagcat tcgcgacgct gctggcaacg acgctacgtt aacgctcgcc agccccgggg
     3601 ctgctaactc gctcggtgct aacgaagcac tcgtgattga caccactgct ccaacagttt
     3661 cgagcgtgaa ttcgccaact gctgctgggt cgtacaacgc tggtgacact atcgatgtcg
     3721 tcgtcaactt caatgaaaac gtaaccgtag ttggtactcc aaccatcgta ttagaaaccg
     3781 gcactaccga ccgcacggtg aactactcct cgggtagcgg cggcaccgca ctcactttta
     3841 cctacacggt gcaagccggt gatacttcgt cggaccttga ttacgtggcc accacttcgc
     3901 tcgcagccgg cactagcatt cgcgatgctg ctggcaacga cgctacttta acgcttgcca
     3961 gccccggggc tgctaactcg ctcggtgcta acgaagcact tgtgatcgac accaccgcgc
     4021 cgactgttac gggcgtagcg tcgaactctg cagctgcgac ttatggtttg accgacgtta
     4081 tcgacgttcg cgtaacattt tctgaaaacg tcaccgttgt tggaactcca actataatat
     4141 tagaaactgg cgccactgat cgcacggtaa actacgtttc cggtaccggc accacagagt
     4201 tgctctttaa ctacacggta caagccggag attcctcggc cgaccttgac tacgttggca
     4261 ctacctcgct taccgccggc accagtattc gcgatgccgc gggcaatgat gccgaccgaa
     4321 cccttgcttc accgggcgca gctgggtcac ttggggccaa tgaggccatc gtgattgtaa
     4381 cgcctactgc tgttccgtcg gatttccctg ggttccaaat cgcctacggt gactttgacc
     4441 gtgacggtga tatcgacgtg gtcattccga gtctttcagc caacgaagtc atcccatatc
     4501 taaacaatgg ttctggtacg ctcagtgctg gtactgcggt tacagtcggt acaaaacctg
     4561 tcgccgtgct cgccgctgac attaaccgcg atgggaaact tgacctcgtt gtttcaaact
     4621 gggatgccga cacagttggc acactgatcg gcgtaggtga cggtacgttc ggcgccatga
     4681 cctccgtggc agtgggcgac aaaccagctg gtcttgccgc gctcgatatc aataaagacg
     4741 gcgatctcga catcgtggtt gcgaactatg gcgccgacaa tgtgcagctg ctttcaaaca
     4801 gtggtttggg aattttaacc gcaggcgcta ctaatgcgac tggcactggt cctacgagcg
     4861 ttgttgccgg cgactgggac cgcgacggtg atcaagacat tgccgtcacg aacttcggtt
     4921 cggggacagt gaccgtgctc gctaccgcac tcggattact gggggcagat gaatcgatta
     4981 ctgttggcac gaatccgttt ggtatcgtgg ttggcgattt taatgctgac ggtaaacccg
     5041 acctcgcggt tgcaaactat ggcgacaacg acgtgagtgt atccctcaat gggggtactt
     5101 cgttcggcgc gaaaactgac accgcagttg gcaccggccc aatcggcatt gcagcggttc
     5161 gctggaatgc aggtggcgcg ctcgatctcg tgatctctaa cagcggtagc gacaactact
     5221 cggttctgag cggtgttggc gacggcacct ttggcgcggc agcaaatact gcattgggca
     5281 gcaccgaccc ttcgggcgtt gccattggcg attttaatcg aaatggcgaa cccgacgtag
     5341 ttttcatgta cagcaccggt gccactgcca cggcccttgc cagtggttcg ggcactaccg
     5401 gcgacggaac ttttaccgta actcaaacgc tttcgtt
//
LOCUS       DAOMXO010000062         5434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_7970_length_5434_cov_4.901283, whole genome shotgun sequence.
ACCESSION   DAOMXO010000062 DAOMXO010000000
VERSION     DAOMXO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7970_length_5434_cov_4.901283"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PLH57_02240"
     CDS             <1..1000
                     /locus_tag="PLH57_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRK01459.1"
                     /translation="FERSWSKEPFMAGKYFPVDIKMFRNSAKDITFDVYLKLAEDNYA
                     HVFSRSSGVDYKRLAQYISKGITHLFVREEDDLLFREYMKKSPDKMLFDPEVPDAKKV
                     SLLLNMAEQNLNELFNVVAIGNEVAASSTKVVKSFITMMGQNPKTLATLLRTASHGEY
                     LFYHSISVAVISMYLAKATGQANPRMLEICGMGGFLHDIGLTMLPKEVVDSPTDLTPA
                     QWREMRSHPKLGVKMIEATPNIPDEVRYIVFQHHEAPDGSGYPGGLRGPVIFYPAKIV
                     GLADSFCALITKRPFREAYTIEQALGILQHEVGKYDRDLVKVLVGLFGDKKKAAAA"
     gene            complement(1023..1607)
                     /gene="def"
                     /locus_tag="PLH57_02245"
     CDS             complement(1023..1607)
                     /gene="def"
                     /locus_tag="PLH57_02245"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRK01460.1"
                     /translation="MTESEVPMAILKVARLGHPVLRKTAKEVSKAEIKTAEFQTFLDD
                     MVDTMREYEGIGLAAPQVHRSQRVSVIELPEEGSARYPNQPPTGLQFFINPKVTILTQ
                     DLGRYWEGCLSVPGMRGLVERPSKVKVDYLNREGKTAEFVADGFIATVVQHEFDHLDG
                     VLYVDRLVSSKNFAFDQEFERYWLPDAEDGELPE"
     gene            1640..2146
                     /locus_tag="PLH57_02250"
     CDS             1640..2146
                     /locus_tag="PLH57_02250"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HRK01461.1"
                     /translation="MAGEFFVKGEKIVLKSNGLWLADGIEITHDQTKALFYRAIHWKP
                     KNFNGPDTYYLTVGYETIAIEVEDTPFFVVAVDCGHQKWTATLTDQETIAIEAEMLEY
                     RGGSLYVNISKTHGDRINAGYPWARFLSAPYHALLSHLEEDAEYYFIAIPGAKNRRAN
                     LLPKGSAH"
     gene            complement(2082..2687)
                     /locus_tag="PLH57_02255"
     CDS             complement(2082..2687)
                     /locus_tag="PLH57_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRK01462.1"
                     /translation="MNKIIFVTLAMVATLFSLADSARAKNKSRRVFGILERSVVLDFP
                     LPYGYVPKLTLSFESKNDFDPMWSKSLTADNWNDFWFDWASKRLLFPTASNKLEIETK
                     VETLSVLGTLRIPKTFDFILTALKDRLEFIEVRKAAIQSLKDWGGFVSARILADQLKL
                     EKDPEIKLALHDALNQIGGRPNERNLSVTSSPDGSSRLEWQ"
     gene            complement(2750..3772)
                     /gene="dusB"
                     /locus_tag="PLH57_02260"
     CDS             complement(2750..3772)
                     /gene="dusB"
                     /locus_tag="PLH57_02260"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRK01463.1"
                     /translation="MLKLGERTINFPFVLAPMAGITNAPFRRLMREMGAGVVVSELIS
                     AAGIEYGGRKTHELCKYFDQERPVGLQIFGETPEHLEKAALYLERLGADFVDINMGCP
                     VPKIVSKGGGAAMLRDPSKLYATLSRLKNAIRIPLTIKIRTGWDACSLNAVEVVHAAA
                     EAGVSWVAIHGRTRAQGYEGSADWDFIAEVKNKSPIPIIGNGDILTAEGAIHRLRTSG
                     CDGVMIGRGALRNPFIFVEIQNLLGKNNTTEHTYWSLIERHLELLRECYVDFYLGVQL
                     RKFLTWYSAGMEGASALRKTLYSFENSDQGTAEVLRVAREFFDRPGLRKQSSYLNEAF
                     LQGGHG"
     gene            3969..4379
                     /locus_tag="PLH57_02265"
     CDS             3969..4379
                     /locus_tag="PLH57_02265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRK01464.1"
                     /translation="MKFETHTYPLTILERHLDSFGHVNNAAYLEIFEEARWDLITRKN
                     YGLDKVQQTKIGPTILEVNLRFHREVKLRQNIVIHTRTENYEGKVGVIIQEMKDESGQ
                     LHCTATLKMGLFDLQARKLIPATPEWLHAVGLIS"
     gene            complement(4385..>5434)
                     /locus_tag="PLH57_02270"
     CDS             complement(4385..>5434)
                     /locus_tag="PLH57_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HRK01465.1"
                     /translation="LVAQIELIQFRTLINSSLLLQGADRFYYSFYKTNQRNGVEHLDQ
                     KILRHLDSYRASQGRSGRGFLPIFDAAELLGEMRIFKSPEELDRLRRAGDITARVHTE
                     VMARTKAGLYEFQIEALLEYLARDAGCERHGYPSIVAGGKNATILHYVENAEKLNDGE
                     LLLIDAGFECDYHTADITRTYPINGKMSAPQQEIYQVVLNVQKECIRLAKPGATLAAI
                     HAFAIDALTDAMIALKFLSGDRKKLIETLAYKRYYPHGTGHLLGMDVHDAGLYQQNGA
                     PRLLEPGMVFTIEPGFYVLHDDAQVPAHYRGIGVRIEDDVVITPEGCEVLTAKAPKEI
                     ADMASVIGTKPWLTV"
BASE COUNT         1462 a         1331 c         1246 g         1395 t
ORIGIN      
        1 atttgagagg agttggtcca aggaaccatt tatggcagga aaatatttcc cagtcgacat
       61 caagatgttt cgaaattcgg cgaaagacat tactttcgac gtctacctta aactcgccga
      121 agacaattac gctcacgtgt tttcgcgcag ttcgggcgtg gactacaaac gattagcaca
      181 gtacatatct aagggaatca cccacctgtt tgttcgcgaa gaagatgatc tactttttcg
      241 tgaatacatg aaaaaatcgc ccgacaagat gctgttcgac cctgaagtgc cggacgcaaa
      301 aaaagtctcg ctacttctca atatggcgga acaaaatctg aatgaacttt ttaacgtagt
      361 agctatcggt aacgaagtcg ccgccagcag taccaaagtt gtaaaaagct ttattacaat
      421 gatgggtcaa aatccgaaaa ccctcgcgac actattgcga accgcaagtc acggcgagta
      481 tcttttttat cattcgattt cggtagccgt aattagcatg tacctggcga aggccactgg
      541 ccaagcgaat cctcgtatgc ttgaaatctg cgggatgggt ggttttttgc acgacatcgg
      601 tctcaccatg ctaccaaaag aagtagtgga ttctccgacc gaccttacac cggctcaatg
      661 gcgtgaaatg cgttcgcatc caaagttggg cgtaaaaatg atcgaggcta ctccaaacat
      721 tcctgatgaa gttcgttaca ttgtatttca acatcacgag gcaccggatg gatcgggcta
      781 ccccggcgga ttgcgcggac cagttatttt ttatcccgca aaaatagtag ggctcgccga
      841 tagtttttgt gcgctaatta ccaaaagacc atttcgggag gcctatacta tcgagcaagc
      901 attgggaatt ttacaacacg aagtcggcaa gtatgatcgc gatctggtaa aggtgctggt
      961 cggtctattt ggcgataaga aaaaagccgc ggccgcgtaa atcaaccaat tcccaaatca
     1021 atttattccg gaagttcacc gtcttccgcg tcgggcaacc agtaacgttc aaattcttga
     1081 tcgaaagcaa agtttttaga actgaccagc ctgtcgacgt ataacacgcc atcgaggtgg
     1141 tcgaactcgt gctgaactac tgtagcaata aagccatcgg ccacgaattc ggcggttttc
     1201 ccttcgcgat ttaaataatc tactttcact ttactcggcc gctccacaag gcctctcatg
     1261 ccgggtacgc ttaaacagcc ctcccaataa cgtccaagat cttgggtaag aattgtaact
     1321 tttggattaa tgaaaaactg gaggccagtt ggcggttggt tcggatagcg ggcgctacct
     1381 tcttctggca attcaatgac cgatacgcgc tgagatcgat gtacttgggg ggccgcaagc
     1441 ccgattccct cgtactccct catggtgtcg accatgtcgt cgagaaaagt ttgaaactct
     1501 gcagttttga tttcagcctt ggaaacctct ttcgcggtct tgcgcaagac tgggtgccct
     1561 aaacgggcta ctttaagaat cgccatcggc acttcgcttt ctgtcatttc tacattagaa
     1621 gattccagag ggagatgcaa tggcaggtga gttcttcgtc aaaggcgaaa aaattgtttt
     1681 aaaatccaac ggtctttggt tggccgatgg gatcgaaatc acccacgatc aaacaaaagc
     1741 ccttttttat agggctattc actggaaacc taaaaacttc aatggtcccg atacttacta
     1801 cctgacggta ggttacgaaa ccattgctat cgaagtagaa gatactccat tttttgttgt
     1861 ggccgtggat tgtggtcacc aaaagtggac tgccacgctc actgatcaag agaccatcgc
     1921 aatcgaggct gaaatgctag agtatcgagg aggatcgctg tacgttaata tctctaaaac
     1981 ccacggagat cgaataaacg cgggctatcc ttgggcacga ttcttaagtg ccccatacca
     2041 cgcattgctt tcccatctcg aagaggatgc cgagtattat tttattgcca ttccaggcgc
     2101 gaagaaccgt cgggcgaact tgttaccgaa aggttccgct cattaggacg accgccgatc
     2161 tggttcagcg catcgtgaag tgctaatttg atttcggggt ctttctctag ctttaattgg
     2221 tcggccaaaa tacgcgccga tacgaaacca ccccaatctt taagtgactg aatcgcggct
     2281 tttcgcactt caataaactc cagtcgatcc ttcaacgcag ttaaaataaa atcaaaagtt
     2341 ttgggaattc gtaacgtacc gagcacgctt aacgtttcaa ccttggtttc tatttccaat
     2401 ttattcgacg cggtcggaaa cagtagtcgc tttgaggccc aatcgaacca aaagtcgttc
     2461 caattatcag cagtcagaga cttagaccac attgggtcaa agtcgttctt actttcgaaa
     2521 cttaaagtca gtttcggcac gtatccatag ggcaacggaa aatctagaac cactgaacgt
     2581 tcaagaattc caaaaaccct acgagactta tttttggctc gcgcggaatc agcgagactg
     2641 aataacgtcg ctaccattgc caaagttacg aagataattt tattcatggt ggaagttcta
     2701 ttgtattcgc aactaaaaga aaagtcgagt tcgcgaacgg tagctacggc tagccgtggc
     2761 caccctgtag aaaagcttca tttaaataac tcgattgttt tcgtaaaccg ggtcgatcga
     2821 aaaactcccg agcaacacga agcacttcgg ccgtaccttg atcgctattt tcaaacgaat
     2881 acaacgtctt tcgcaaggct gacgcgcctt ccatgccggc agaataccaa gtcaaaaatt
     2941 ttcgtaattg cacacccaaa taaaaatcga cgtaacactc acgtaggagt tcaaggtggc
     3001 gttcgatcag tgaccaatag gtgtgttcgg ttgtattatt ttttcctaag agattttgaa
     3061 tctccacaaa aataaacgga ttgcgaagag ctccacgacc aatcatcact ccgtcacagc
     3121 cgctggtgcg cagtcgatga attgcgcctt ctgctgtgag gatgtcgcca ttgccaataa
     3181 ttggaattgg ggatttattt ttcacctcgg ctatgaaatc ccaatcggcc gagccctcat
     3241 aaccttgcgc tcgagttcgt ccatgaatgg ccacccaaga aacgcctgcc tctgcggcgg
     3301 catgcactac ttcaactgcg ttgagcgagc aagcgtccca accggtgcga attttaatcg
     3361 taagtggaat gcgaatggcg ttttttagcc gactgagggt agcgtacagc ttactggggt
     3421 cgcgcaacat ggcggctcct ccacccttac ttacaatttt gggaaccggg catcccatat
     3481 taatatctac gaagtcggct ccgaggcgtt ccaagtaaag cgcggctttt tcaaggtgct
     3541 cgggggtttc gccgaaaatt tgcaacccaa ctgggcgttc ctggtcgaag tatttgcaca
     3601 gctcgtgagt tttgcgtccg ccatattcaa ttccggcggc ggaaatgagc tcgctgacca
     3661 ccacaccggc tcccatttcg cgcattaatc gtcgaaatgg ggcgttagta ataccggcca
     3721 ttggagccag aacgaacgga aaatttatgg ttctttcgcc tagtttcagc actttaaatg
     3781 atccttgccg aaacgttcgg cgcccaggat atagcatgtt ttagcgcaaa tagtgaccga
     3841 aaatctgggt ttaacccagg gcgacggtat cattgggaaa ttgcacctaa ccgtgcctgg
     3901 gagggttcaa ttagagcagc ggggcccctc ttggcgaatg ggcaagagca tgcaaaactc
     3961 catctattat gaaatttgaa acccacacct accccctcac tattcttgaa cggcacctcg
     4021 attcgtttgg tcacgtgaac aatgcagcct atttagaaat ttttgaagag gcccggtggg
     4081 atttgatcac ccgtaaaaac tacggattag acaaggttca gcagactaaa attggcccca
     4141 cgattcttga agtgaatttg cgctttcatc gcgaagtaaa gttacgccaa aacattgtca
     4201 tacacactcg caccgaaaac tatgaaggca aggtcggcgt gattatacaa gagatgaaag
     4261 acgaatcggg acagctacat tgtaccgcca ctctcaaaat gggcttattc gaccttcagg
     4321 cccgcaaact gattcccgct acgccagaat ggctgcacgc ggttgggtta atttcctaac
     4381 caaatcacac cgtcaaccac ggtttggtgc caattactga ggccatatca gcaatttctt
     4441 ttggcgcttt cgcggtcaac acttcgcagc cttcgggcgt aattacaacg tcgtcttcaa
     4501 tgcgcacccc aatgccacga taatgagcgg gaacttgtgc atcgtcatga agcacataaa
     4561 aaccaggttc gatcgtaaac accatgcctg gctccagcaa acgtggagcc ccattttgct
     4621 gatatagtcc agcatcgtgc acgtccattc ccagtaaatg accggtgccg tgcgggtagt
     4681 aacgcttgta ggctaaagtc tcgatgagtt tcttacgatc gccagaaaga aacttcaatg
     4741 cgatcatcgc gtctgtcaag gcgtcgatcg caaatgcgtg aattgcggca agtgtagccc
     4801 ctggctttgc tagacgtatg cattcttttt gaacatttaa tacgacttga taaatttcct
     4861 gctgtggcgc cgacattttt ccgttgattg gataggtgcg tgtgatgtct gcagtatggt
     4921 agtcacattc gaaaccggca tcaattaaaa gcaattcgcc gtcattaagt ttctcggcgt
     4981 tttctacgta atgcaaaatt gtggcatttt tccccccagc cacaatcgag ggataaccgt
     5041 gccgctcgca acccgcgtcg cgcgcaagat attcgagcag cgcttcgatt tgaaactcgt
     5101 acaatcctgc cttagtgcgc gccatgactt cggtgtggac tcgcgcagta atatcgccgg
     5161 cacgacgtag acgatccaat tcttctggcg atttaaaaat gcgcatttca ccaagcaatt
     5221 cagcggcatc aaagattggc aaaaaacccc ggccagagcg gccctgactc gctctatatg
     5281 aatctaaatg cctaagtatt ttttgatcca aatgttctac gccattgcgt tgattggttt
     5341 tgtaaaaaga gtaataaaat cggtctgcac cttgtaacaa tagggagctg ttgatcaaag
     5401 tccgaaattg aataagctcg atctgcgcca caag
//
LOCUS       DAOMXO010000063         5399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8048_length_5399_cov_5.283496, whole genome shotgun sequence.
ACCESSION   DAOMXO010000063 DAOMXO010000000
VERSION     DAOMXO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8048_length_5399_cov_5.283496"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..505
                     /locus_tag="PLH57_02275"
     CDS             53..505
                     /locus_tag="PLH57_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRK01466.1"
                     /translation="MADPIGTAYYRRLPGIDVAGKTGTSQVVRMAPGKVEQKCESMPF
                     KFRDNALFAAFAPPDDPVIAVAVVAEHACHGSSGASPIAMEVIKTYLQKYMPERYSDE
                     AIKARLKQGNLARSIQIKSTLESEEDGVRLNDFVEPHKEPLPDPEGTH"
     gene            505..1605
                     /gene="rodA"
                     /locus_tag="PLH57_02280"
     CDS             505..1605
                     /gene="rodA"
                     /locus_tag="PLH57_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRK01467.1"
                     /translation="MEEERFRQFDWSLILISVVIFIVGVLNLYAATFAEYSGKGLRLY
                     QTQLLWFGIGSTAAIGLLLVHYKMLSRAAYIVYFLNLFLLVLVLVIGKQSLGARRWIG
                     FGGLYIQPSEFMKLSAVLAMAKYFETDHTTAGYRLRDLWLPGMLIAVPVGLIIMQPDL
                     GTAMILLLTFSSMMLFIKVKKKSLVILALVGLITAPVIYKYGLKQYQRQRIISFLNPM
                     SDPKGSGYNSIQSMIAIGSGQFMGKGFKKGTQSQLNFLPEHHTDFIFSVFSEEHGFIG
                     ALVLLALYLLFFMTALNIAYVSHDKFGTLLALGVLFIFFWHVVFNIGMVCGLLPIVGV
                     PLPFMSYGGSSLITSILGIAILTNISNKRFMF"
     gene            1619..1963
                     /locus_tag="PLH57_02285"
     CDS             1619..1963
                     /locus_tag="PLH57_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01468.1"
                     /translation="MNRKIKAQWILGKVLRRLVMVFIVLTFFACKGDDQLKGQVSDLT
                     AKLEEANKKISDQDGALKSATGEIKELKELVAKLGNAVVDLRKANEPKKKVAVKTSKK
                     TPSKKPSHRRGH"
     gene            2034..3608
                     /locus_tag="PLH57_02290"
     CDS             2034..3608
                     /locus_tag="PLH57_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01469.1"
                     /translation="MHYRRVPKGLFFFFLYLNFLVFSGCGKEQDEKATNSSSNVTIDQ
                     INSARPVPPLEQLSQVEPSPAPGSKVLGTPSRFEKLLAVLGEFGIDDESSYRHLVREF
                     SELTTRREFEARLKYIDPQNELTRYAIWDDHQLSLYRDRDSKRIKDFSIRFAPEDHSR
                     ELRTVNDLTTKIAALRENSVDLPLKGLRVAIDAGHMHSGEWDSITGKYVKMNDKFVSE
                     GFITFATAQAFSRELRNLGAETLLIRDSADAVTKESFSEFDLTPYAHAELLAATDRAW
                     FDSILSIFDIGADLFSAFENSEDRKRIYSESRRVSVFTHRVDLAARIERALEFKPDIT
                     VIIHYDAWSWGQNPPDFNESRTYIMGNVMPGEAASREDRALILRELLNPVRWDLSLDM
                     SKHMVQSLSKRVDVPVKNSPTDFGSIRVEDGVYARNLGLTRRIYHGLLTYFECMMYEN
                     PKEFEMMSTHDSRYGDYPARVDAVAEGLTRGVLNFVGHQSVVYGEIPESTPLPSPTVA
                     PAPEQPEATPEAAPIL"
     gene            3619..4572
                     /locus_tag="PLH57_02295"
     CDS             3619..4572
                     /locus_tag="PLH57_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRK01470.1"
                     /translation="MQSKPWFSYNDYLQKRFGEKVYKVTVSAGFTCPTRDGKRGSDGC
                     AFCDEHGSGSFFSAGQGTAAIRDQIVQAIPAIRQRFNANKFLAYFQSFTNTYAPVKYL
                     QEVYDGAIGIPDVVGMAVGTRPDCIAEPVLNLLDRYAVGGQYVSLELGLQSLRDDALN
                     FYTRGHTAEEGVDGIRRALKHKNLAVCVHLMFGAPGDNLQTAIDAAQTMNDLGVHGVK
                     LHQLMILKNTGLATRYAREPWPLFTLEQYNELACAFLENLDPNIYVERTHALATHPED
                     LVAPDWASKRWEPFNDLVRRLTKAAETRPSQFGSRDLSRIQ"
     gene            complement(4487..>5399)
                     /locus_tag="PLH57_02300"
     CDS             complement(4487..>5399)
                     /locus_tag="PLH57_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01471.1"
                     /translation="LQNENNFQILPNPQVTKISFSTDGQDFWMFEQSSGNLRRYSNIP
                     LTESAGATVNSKKSDFEYNVPSPVPSVRSIVGLGNGSAALEFENYIRVVGEDIRLTVA
                     LDPLRRHLHARNLGGLVAGSLYYVHRLRGGALLSLTFERAGTFIFRRSRREKRWVVHS
                     WFGRPDVGPTGAIGVRHIIESPVGRPVEDRIFARPSTPVRAGFPRIGAPRPIVGQAPL
                     AGGWLDNETIWRVDVAGNFEALRQYDGQYKLLARFKIETWVPDSSAIFRKRDLVSEVI
                     IEYDSGHVNRIDLDAFRQLLSAGEQDH"
BASE COUNT         1392 a         1352 c         1251 g         1404 t
ORIGIN      
        1 ggtataagtg ccgctgggca ggttgttgat gttgtagaag aagttgttgg tggtggccga
       61 tccaattgga acagcttatt atcgccgtct gccgggtatc gacgtggctg gcaagacggg
      121 tacctcgcaa gttgttcgta tggctcctgg taaagtagaa caaaagtgtg aatcgatgcc
      181 ctttaagttt cgcgataacg cgttgtttgc ggctttcgct ccccccgatg acccagttat
      241 cgcggtcgca gtcgttgccg aacacgcctg tcacggtagc agcggcgctt cgccaatcgc
      301 gatggaagtc atcaaaactt acctgcaaaa atacatgcct gagcgctata gcgacgaagc
      361 gatcaaagct cgcttaaaac aaggtaatct cgcacgttca attcaaatta aatcaacgct
      421 ggagagcgaa gaagacggcg tccgcctcaa cgatttcgtc gaacctcata aagaaccgct
      481 ccccgaccca gaaggaactc actaatggaa gaagaacggt ttcggcagtt tgattggtcg
      541 ctcattctta tttcggtggt tatttttatt gtaggcgttc tgaatctcta tgcggcgacg
      601 ttcgcggaat atagcggcaa gggtcttaga ctatatcaaa ctcaactctt gtggttcggc
      661 ataggaagta cggcagcaat tggcttgttg ctagtgcatt acaaaatgct ttctcgcgca
      721 gcttacattg tttattttct aaacctattc ttacttgttt tagttttggt gattggcaaa
      781 caaagcctcg gggctcggcg ttggatcggt ttcggaggac tttatattca accgtcggag
      841 ttcatgaaac tctccgcagt actcgcaatg gccaaatatt tcgaaacaga ccatacaacc
      901 gcgggctacc gcttgcgcga cctttggctc cctggaatgt tgatcgcagt tcccgtggga
      961 ctgatcatca tgcaaccaga cttaggtacc gcgatgatct tgctgcttac tttttcgagc
     1021 atgatgcttt tcattaaagt taagaaaaaa agcctcgtga tattggcgct cgtcggactt
     1081 atcactgcac cagtaattta taagtacggc ctcaaacaat accaacggca gcgaataatt
     1141 tcgtttttaa atccaatgtc agaccccaaa ggttcgggct acaacagtat ccaatccatg
     1201 attgcaattg gaagtggcca gttcatggga aaagggttca aaaaaggaac tcagtcgcag
     1261 ctcaattttc tccctgaaca tcacactgat ttcatttttt cagtctttag cgaggaacac
     1321 ggttttatcg gagcattggt tttgctcgcc ttgtatttgc tgtttttcat gaccgcatta
     1381 aatatcgcct atgtgtcaca tgataagttt ggtacacttc tagcgttggg ggtgctgttt
     1441 atcttctttt ggcacgtggt cttcaacatc ggaatggttt gcggtttatt gccgatagtc
     1501 ggagttccac ttccatttat gagttacgga gggtcgtcac tgatcacctc catattagga
     1561 atcgccatac ttacgaatat tagtaataaa cgatttatgt tttaaggaaa gaacatcgat
     1621 gaaccgaaaa attaaggccc aatggattct cggaaaagtc cttcgccgcc tcgtgatggt
     1681 attcattgta ctcacgtttt tcgcttgtaa aggagacgac caactaaagg gccaagtctc
     1741 tgatttaaca gcgaaactag aagaagctaa caaaaaaatc agcgatcaag acggcgccct
     1801 aaagtcggct actggtgaga tcaaagaact taaagagctg gtcgcgaagc tcggcaatgc
     1861 agtggtggac ctacggaaag ccaatgaacc taaaaagaag gttgccgtca aaacctccaa
     1921 aaaaacccct tcaaagaagc caagccacag gcgtgggcat taggtcaatg ctccgaattc
     1981 gcggctttat ttaacctttt tcgtcggttt tttttatgat atgaccgcgc cctatgcatt
     2041 atcgtagggt gccgaagggc ctgttttttt tcttcctcta cctcaacttt ttggtgtttt
     2101 ccggctgtgg caaagaacaa gatgaaaagg ccactaacag ttcatcaaat gttacgattg
     2161 atcagatcaa tagcgctcgc ccagttcccc cgctcgaaca gttgtctcag gtggaacctt
     2221 ctccggcccc tggttccaag gtactcggaa cgccttctcg cttcgaaaaa ttattagctg
     2281 tcttaggtga attcggaatc gacgacgaaa gttcctatcg ccatctcgtt cgtgagtttt
     2341 cggaactaac gacccgtcgc gaattcgaag ctcgattgaa gtatatcgat cctcaaaatg
     2401 aactcactcg ttatgccatc tgggacgatc accaactatc tctttaccgc gaccgtgact
     2461 ccaaacgcat aaaagatttt tcaattcgtt ttgccccaga agaccactcc cgggaactgc
     2521 gtacggtaaa tgatctcact accaaaattg cagcattacg cgaaaactcg gtggatttac
     2581 cgcttaaagg cctgcgcgtc gcaatcgacg caggccacat gcatagcggt gaatgggaca
     2641 gcatcaccgg caagtacgtc aaaatgaatg acaaatttgt aagcgaaggt tttattacgt
     2701 tcgccaccgc tcaagcgttt tcccgcgaac ttcgtaatct tggcgcggag actttactca
     2761 ttcgagattc ggccgatgcc gtaaccaaag aaagctttag tgaatttgat ctcacgccct
     2821 acgctcatgc cgaattgctt gcagctaccg atcgagcctg gtttgattca attctctcga
     2881 tctttgatat aggcgctgat cttttttcgg cgtttgaaaa ttctgaagat cgaaaaagga
     2941 tttatagtga atctcgccgc gtaagtgtct ttactcatcg agtggattta gccgccagaa
     3001 ttgagcgcgc acttgagttc aaacctgata ttacggtcat tatacattac gatgcttggt
     3061 cttggggcca aaacccccct gactttaacg aaagtcgcac ttacatcatg ggcaatgtta
     3121 tgcccggcga agcggcatcc cgtgaagatc gcgccctgat tctccgagaa ctgttgaatc
     3181 ccgtgcgttg ggatctttcg ctcgacatgt ctaaacacat ggttcagtcg ctctccaaac
     3241 gagtggatgt acccgttaaa aacagcccga ctgacttcgg atcaattcgt gtagaagacg
     3301 gtgtgtatgc tcgcaacctt ggacttacgc gacgcatcta ccacggcctt ctcacctact
     3361 tcgaatgcat gatgtacgaa aatccaaaag aatttgagat gatgagcacc cacgacagcc
     3421 gctacggcga ttatccggcc agagtagacg cagtagcgga gggcttaact cgcggagtgc
     3481 tgaatttcgt agggcaccaa tccgtggtgt atggtgaaat tcccgaatca acaccacttc
     3541 cctcacctac tgtggcgccc gctccagaac aaccggaagc gactcctgaa gccgctccaa
     3601 ttttataggt gcaaacgggt gcaatctaaa ccgtggtttt cttacaacga ctatctccaa
     3661 aagcgattcg gcgaaaaagt ttataaggtc acagtgtctg ccgggttcac ttgtcccact
     3721 cgtgacggca aacgcggttc agacgggtgt gcgttctgcg atgaacacgg gtcgggttcc
     3781 tttttttccg ctggccaggg caccgctgca attcgcgatc aaatcgtgca agctattcca
     3841 gctattcgac agcgctttaa cgctaataaa tttttggcct actttcagtc cttcaccaac
     3901 acctacgcgc cagtaaaata tctccaagaa gtatatgacg gggcaatagg cattcctgac
     3961 gtagtcggca tggcggttgg caccagacct gattgtattg ccgaaccggt tctaaacttg
     4021 ctcgaccgat acgccgttgg cgggcaatac gtaagtcttg agttaggcct tcaaagtctg
     4081 cgtgatgatg ctttgaactt ctatactcgg ggccatacgg ctgaagaagg tgttgatggc
     4141 attcgtcgcg cgctcaaaca caaaaatctc gcggtctgcg tgcacttgat gttcggcgca
     4201 cctggtgaca acttgcaaac agctatagac gccgcccaga cgatgaacga tctcggcgtg
     4261 catggtgtaa agctacatca attgatgatt ctcaaaaata ccggtctggc cacacgttac
     4321 gcccgtgaac cttggccgct gtttactctt gagcagtaca atgaacttgc ttgtgctttt
     4381 ttagaaaatt tagacccgaa catatacgtc gaacgaaccc atgctttagc cacgcacccc
     4441 gaagatctcg tcgctccaga ctgggcgtcc aaacgctggg aaccattcaa tgatcttgtt
     4501 cgccggctga caaaagctgc cgaaacgcgt ccaagtcaat tcggttcacg tgacctgagt
     4561 cgtattcaat aatcacttcc gaaacgagat ctcgctttct gaatattgca ctggagtcag
     4621 gcacccaagt ctcaatttta aagcgtgcta ataatttata ctgtccatca tactgacgaa
     4681 gagcttcaaa atttcctgca acatcaaccc gccaaatcgt ttcattgtcg agccagccgc
     4741 ctgccaaggg tgcttggccg actattggac gaggcgcgcc aattcgcgga aaccccgctc
     4801 gaacgggcgt cgatggtcgc gcaaaaattc gatcctcgac cggacggcct acgggcgact
     4861 caatgatatg acgcactccg atcgctccgg taggaccaac gtctggccga ccaaaccagg
     4921 aatgtactac ccaacgtttc tctcgccgag atcttctaaa aataaaggtt ccggcccttt
     4981 caaacgtcag cgacaaaagc gccccacctc tcaagcgatg aacatagtac aaactgcccg
     5041 cgactaaccc gccaaggttt ctggcgtgaa gatgcctgcg aagtgggtct agcgctacag
     5101 ttagacgaat gtcctcgcct acaacacgaa tatagttttc gaactccaga gcagcggacc
     5161 cgtttcctaa ccctacaatg gaacgtaccg agggcaccgg ggaaggtacg ttatactcga
     5221 agtcactttt tttagaatta actgtagccc ctgccgattc cgttaacgga atatttgagt
     5281 aacgacgtaa gttgccactg ctttgttcga acatccaaaa gtcctgcccg tcggttgaaa
     5341 acgaaatttt cgtaacttgg ggattcggca aaatttgaaa attattctca ttctgcagt
//
LOCUS       DAOMXO010000064         5360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8124_length_5360_cov_4.406032, whole genome shotgun sequence.
ACCESSION   DAOMXO010000064 DAOMXO010000000
VERSION     DAOMXO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8124_length_5360_cov_4.406032"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PLH57_02305"
     CDS             complement(<1..1042)
                     /locus_tag="PLH57_02305"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRK01472.1"
                     /translation="MNDAINFYRGKQVLVTGGAGFLGSNLATRFVQNEIAVTVVDNFH
                     PDYGANPFNLEGLGSNLNLIRGDITDRALMKELCGRADVIYHIAAQCSHVDSMIDPWL
                     DLKFNCEGTLAVLEGAKASAKKPVVVYAGTRAIIGAPLESPAGESTLPNPVDVYGVNK
                     LAAEWYGAVYARVHGIPYVSLRLTNCFGERHQMKNGKYGILNWFMSLALQGKTIKIYG
                     TGAQLRDYLYVSDAIDSFVAAGRFGEKLRNGESFPEVQLSGEKVPFAIFNVASGKPKA
                     FAECAKRVATIGGGSVEFVPWPKDREAIETGDFVATITAAERYLGWRPKVEFEAGLQN
                     TFAYYRQHLSRYL"
     gene            1072..2097
                     /locus_tag="PLH57_02310"
     CDS             1072..2097
                     /locus_tag="PLH57_02310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRK01473.1"
                     /translation="MRHKILIIKLAALGDIASTSRAVAEVFGALPKDSEIHWVLDAGY
                     HKLAQSMTSILGCDGIQFHAFNTERLFRGSVPNKLVATAGLLSKCKAIGPSAIVLLHR
                     DFRYKLALRAVFRVPIFGLHLSIENEYDMYFDALSRLRSHYFSAHTPITNISEFQQFK
                     KGHVGILVGGAANTKGTFFEKRWPHLKNFIEKLVLKQNFKISLFGGKEDVSLANEILA
                     SETLRNANCSVENFVGLLDLDQIPNALQSLQYFVSIDSGLGHIAAAVMTAPEQKVISL
                     FGPTDPRIWAPKPRTPNQMNIVYRGISCSPCYKNSGLFSPCPYTGENFQRCMKEISPD
                     HILDLIT"
     gene            complement(2080..3165)
                     /locus_tag="PLH57_02315"
     CDS             complement(2080..3165)
                     /locus_tag="PLH57_02315"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRK01474.1"
                     /translation="MNTLNLPNLAPIGVSILKAAQAVDATVLITGATGTGKSHLAEQI
                     HHADTRRGGRKLIKVNLATLSENLIESELFGHERGAFTGADFRRVGKLESADGGTVFL
                     DEIGEMPVRLQSKLLDFIQYKKITPVGSNRELNLNVRILAATNRNLEAAVKAGSFRED
                     LFHRLNVFRVHLSDLANTPQVALDMARELILENLRRLNKPDMKISRDLQSVIRHYPWP
                     GNIRELRNALEFAVAMEPTAELQLTSMPVPIQTWVREGGLGRKEELPVLMERPMPATQ
                     GAGFYASSSILELPVNLNFKESKAQFEKRYIEYILRACNGQINLTSRRAGLNKVSLTE
                     KIKIYGIDWRRIRFESTKAADVSRNQI"
     gene            3436..4821
                     /locus_tag="PLH57_02320"
     CDS             3436..4821
                     /locus_tag="PLH57_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRK01475.1"
                     /translation="MLARKKKILLVDDDRNIHLANKYILKDYYECISAYDGDEARIIL
                     QSQSVDLILLDIHMRKYEEGLEFIPIFREIDPDIDIVISSSNKELELAARAVKAGASA
                     YLIKENALEQLLITVESVLSKRELIRQNQHLFKDRKRSLEKASIIGESKAMKKLLQDI
                     EKIRRVQANVVITAETGCGKELVARHIGVVDGAPFVTVDSSTITHSMAESMLFGHEKG
                     AFTGAVAQSKGLFEEANGGVIYFDEISNMPLDIQTKLLRVVQEKEIMRLGSTKIIPLS
                     FRVICATNRDLEKMVGEGKFKEDLFQRLNVVTLSIPPLRDRLEDFSLLVESFFKKHGD
                     EASPKRFSDSATELLLKYSWPGNVRELSNLIANMCALIHGQEEVEVEDLPERLRIQTT
                     HMLRIAPPAQEPTNIDINEDFYEYMRRLEGKALHGLYQSFSGNISAMSKKLKMSRSHL
                     YSKLYAHGIHK"
     gene            4938..5168
                     /locus_tag="PLH57_02325"
     CDS             4938..5168
                     /locus_tag="PLH57_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01476.1"
                     /translation="MANQEHSKKKTDTNRTLTTVARRQELEFEADVLFQRLGNKWYAF
                     SVVEDDCLMTEVSEEEVQKTQKRMMKRGARAA"
BASE COUNT         1488 a         1294 c         1157 g         1421 t
ORIGIN      
        1 acaggtatcg tgaaagatgt tggcgataat aggcaaaagt attttgtaag cccgcctcaa
       61 actcaacttt tggtcgccag ccgagatatc gctcagcagc agttatggtt gctacaaaat
      121 ctccggtttc gatagcttct cgatcttttg gccacggaac aaactctacg gacccaccac
      181 caatggtggc cacacgcttg gcgcattcgg caaacgcttt tggtttgcct gatgcaacgt
      241 taaagatagc aaacggaact ttctctcccg aaagctgcac ctctgggaag gactccccgt
      301 ttcgcaattt ctccccaaat ctcccggcgg cgacaaagga atctatcgcg tcagacacgt
      361 aaaggtaatc tcgtaattgc gcaccagtcc cgtaaatttt aatagtttta ccctggagag
      421 ccagactcat aaaccaattc aaaataccgt atttaccatt tttcatctga tggcgctcgc
      481 caaaacagtt cgtcaaacgg agtgaaacgt acggaatacc atgaactcgt gcgtaaaccg
      541 ctccgtacca ctcggcagcg agtttattaa cgccgtaaac atcgacaggg tttggaagag
      601 tactttctcc agcaggtgat tctagcggcg cgccgatgat cgcgcgagtt cccgcgtaaa
      661 ccacaacggg cttcttagca ctggcctttg cgccttccag aaccgccaaa gttccttcac
      721 aattgaactt taggtcaagc caggggtcaa tcatactgtc gacatgagag cattgcgccg
      781 ctatgtgata tatcacatcg gcccgcccac agagctcctt cataagagcg cgatccgtga
      841 tatcgccacg aattaggttt aaatttgacc cgagcccctc tagattgaag gggttcgcgc
      901 cgtagtcggg gtgaaaatta tctaccactg ttacggcgat ctcgttttga acaaaacgcg
      961 tcgccagatt gctgcccaag aatccggccc cgcccgttac aaggacctgc tttccacgat
     1021 aaaagttgat tgcgtcgttc atttattatt tctataattt tttttgatta aatgcgccat
     1081 aaaatactca ttatcaagct tgcggcacta ggagatattg catctacctc gcgggctgtc
     1141 gccgaagtgt ttggtgctct acctaaagat tccgagattc actgggtcct cgatgcgggc
     1201 tatcacaaat tagctcagtc catgacttcg atacttggtt gtgacggtat tcaatttcat
     1261 gccttcaaca ctgaacgact atttcgcggt tctgtaccga ataaattggt ggctaccgcc
     1321 ggacttttgt cgaaatgcaa agccatcggg ccttccgcaa tagttttact ccaccgggac
     1381 tttcgttaca aactagcttt gcgcgccgtc tttcgagttc ctatatttgg attacatctg
     1441 tcgatcgaaa acgaatacga catgtatttc gatgcattgt cgcgtctacg atcacactat
     1501 ttttctgcgc acactccgat tacaaacatc tcggaatttc aacaattcaa aaagggtcac
     1561 gtaggaatac tagttggcgg tgcagcgaac actaagggaa ctttttttga aaagcgttgg
     1621 ccccacctaa aaaacttcat cgaaaaatta gtactcaagc agaatttcaa aatatcgctt
     1681 tttggtggca aagaagatgt tagtttggcc aatgaaattc tcgcttctga gacgctgcgc
     1741 aatgctaatt gttcggtcga aaattttgtt ggattacttg atttggatca gatcccaaac
     1801 gccctgcaaa gccttcaata ttttgtctct attgattccg gccttggtca tattgcagcc
     1861 gcagttatga ccgcacctga gcagaaagta atctctttgt ttgggccaac cgaccctcgg
     1921 atttgggcgc ctaaaccgcg cacacccaat caaatgaata tcgtttatcg cggcatttcc
     1981 tgttccccgt gttacaaaaa cagcggactt ttcagccctt gcccctacac aggcgagaac
     2041 tttcaacgct gcatgaagga aatctcacct gatcatatac tagatttgat tacgtgacac
     2101 atcggctgct ttagtagatt cgaagcgtat tcggcgccag tcgatgccat aaatttttat
     2161 tttctcagtt aacgatactt tatttaggcc cgcccgacgg ctggtaagat tgatttgtcc
     2221 gttgcaagcc cgcaaaatat actcaatata ccgtttttca aactgggcct tcgattcttt
     2281 aaagttaaga tttactggta actcaagaat gctcgacgaa gcgtaaaatc ccgcaccctg
     2341 ggtcgctggc atcggtcttt ccattaaaac cggcaattct tccttgcgcc caagaccacc
     2401 ctctctcacc caagtttgta ttggcacggg catagatgtt agttgcagtt cggctgttgg
     2461 ttccatggct accgcaaatt ccaaagcatt acgaagttca cgaatgttac ccggccaagg
     2521 atagtggcga ataacggact gtaggtctcg cgatattttc atgtcaggct tattcaaccg
     2581 tcgtaggttt tcaagaatca actctcttgc catgtcgagt gccacctgcg gggtgtttgc
     2641 taaatcgctt aggtgaactc gaaatacatt taatctatga aagaggtcct cgcgaaagga
     2701 accggcttta accgccgcct cgagattacg atttgtagcg gccaatattc ggacgtttaa
     2761 atttaattct cgattagagc caaccggagt aatttttttg tactgtataa aatccaaaag
     2821 cttagactga agacgtactg gcatttcacc aatttcatcc aaaaatactg tgccaccatc
     2881 tgcactttcc agttttccaa ctcgccgaaa gtcagccccc gtaaatgcac ctcgctcatg
     2941 cccgaagagt tccgattcaa ttaaattttc gctcagagtg gcgaggttta cctttatcaa
     3001 cttacggcca ccgcggcgcg tatcggcatg gtgaatctgc tcagccaggt gtgacttgcc
     3061 ggttccagtg gcaccagtaa ttaaaacggt ggcatcaacc gcttgtgcgg ccttcaatat
     3121 gctaactccg attggcgcca aattcggtaa atttaatgta ttcataaaat cgaactcctc
     3181 tactttcttg acgtgccgct tcagcaaatg tggtgccacg aacttttcac ctaaaaaggg
     3241 aggggctcgg tttcgctttt tccggaattc ggccccgatt gtacgcgatc gcatagtgta
     3301 ttgttttccg tacagtgtct gtattttatt cagagccctt gaggtgcctg aatatattgt
     3361 cctttaaaaa caggcactta tatttttacc cctagggcac ggactttgct ctttaacacc
     3421 ttgaggtaaa aaccaatgtt ggctcgtaaa aagaaaattc tattggtcga tgacgatcga
     3481 aatattcatt tagctaataa atacattctt aaagattact acgaatgtat tagcgcttac
     3541 gacggcgatg aagcgcgcat cattttgcag tcgcaatcgg tcgacctcat cttactcgac
     3601 attcacatgc gaaaatatga agagggtctc gagtttattc ctatcttcag agaaattgac
     3661 cccgatatcg acatagttat ttcaagttcg aataaagaac tcgaattagc cgctcgcgcc
     3721 gtcaaagcgg gggctagcgc ctatctcatc aaagaaaacg ccttagagca gcttcttatc
     3781 acggttgagt cggttttaag caaacgcgaa ctcattcgtc agaaccagca cctttttaag
     3841 gatcgtaaac gttcgctaga aaaggcttcg attattggcg agtcaaaggc catgaaaaaa
     3901 ctgctccaag atatagaaaa aatccgccgc gttcaggcaa acgtcgtgat caccgccgaa
     3961 accggttgtg gcaaagaatt agtagcccgg catataggcg tggtcgacgg agcgcctttt
     4021 gtgaccgtcg acagttccac cattacccat tccatggcag agagtatgtt atttggtcac
     4081 gaaaagggtg cgtttacagg cgctgttgct caatctaaag gtctttttga agaggcaaat
     4141 ggcggcgtga tttattttga cgaaatatca aacatgcccc ttgatattca aactaaactg
     4201 ctgcgagtag ttcaggaaaa ggaaattatg cgattggggt ccaccaaaat aatccctctc
     4261 tcatttcgag tcatttgcgc cacaaatcgt gacctcgaaa aaatggtggg tgaaggaaaa
     4321 tttaaagaag acctattcca gcgcctcaat gtagtaacgc tttctattcc accgctaaga
     4381 gatcgtctcg aagacttttc tttattagtc gagagttttt tcaagaaaca cggggatgaa
     4441 gcgagtccta aacgattttc agattctgct actgaactgc tattaaagta ctcctggcca
     4501 ggaaacgtgc gggaactttc taacctaata gcaaatatgt gtgcgcttat tcatgggcaa
     4561 gaagaagtgg aagtggaaga cctgccggaa cggttgcgca ttcaaacgac acatatgctc
     4621 agaattgctc ccccagcaca agaacctact aatatcgaca tcaatgaaga cttctacgag
     4681 tacatgcgcc gactcgaagg aaaagccctt cacggactat atcaatcatt ttcgggcaac
     4741 attagtgcga tgagtaaaaa actaaagatg agccgcagtc acctttactc taagctctac
     4801 gcccacggca tacacaaata agcactgcct atcggaaaaa attgcccact ttggattttt
     4861 ggcaatatga cgatgtgtaa tttgtaggtc gccccagttt gcgaccgctt tgttacagcc
     4921 aaatccagga gggaaccgtg gcaaaccaag aacactctaa gaaaaaaacc gacacgaacc
     4981 gcacactgac taccgttgcg cgtcgacagg agctcgaatt cgaggcggac gtactatttc
     5041 aacgccttgg aaataaatgg tacgctttca gcgttgttga agacgactgc ctgatgactg
     5101 aggtttcaga agaagaagtt caaaaaaccc aaaaaagaat gatgaagcgc ggcgctcgcg
     5161 cagcttgaaa agtttgtata aatgccttgg agaccacaac cacgaaaaaa aggcatgaag
     5221 tccaccgaag gaacacgagc gcccgaaaag gacgctcgtg tttcgtcgag tgacgatata
     5281 aaaaaacaaa tcgctatttt atctgaaaaa attaattcca agctaaaaac cgaaacatcg
     5341 gtaaaaaagg ctgcccttat
//
LOCUS       DAOMXO010000065         5358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8129_length_5358_cov_4.300962, whole genome shotgun sequence.
ACCESSION   DAOMXO010000065 DAOMXO010000000
VERSION     DAOMXO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8129_length_5358_cov_4.300962"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1274
                     /locus_tag="PLH57_02330"
     CDS             132..1274
                     /locus_tag="PLH57_02330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRK01477.1"
                     /translation="MNSRRIVHSCTSESFSGLERYVLDLALEQYRTGLNVELFCREGT
                     ELFESAHERGLPIWSLNSKTKKGPAAWAEIRREWHRRLSAGDMVLHMHAGGEPWFHLP
                     WLLTRPNTLKKTILQYHIWINHSKKDPLHWAVFRGIDEVWCSSETARVHLSSLLPVPR
                     DHFRVVFYGRDVARLSDPSQITWRREIRERFSIPENEILGVCVSRIEPIKGIAELFDA
                     FVNYASRHKTGTLLLVGDTSPNDESAERLGSELKSRHTRLPENIRSRLLMPGYLGEIE
                     KVLAASDFYVVPAYEECNSLSMIDSAVMGKAILGTNSGGTPSVVRPGETGELVPPKDA
                     AAMERGLEKLFLDSDLRTRCGLGAKALAPTFDRRRIFQEIQRAYEQ"
     gene            complement(1278..4037)
                     /locus_tag="PLH57_02335"
     CDS             complement(1278..4037)
                     /locus_tag="PLH57_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRK01478.1"
                     /translation="MQKIFGRLAVVSVLFFLSISLSSLESFASADSTQPPNQLWIGEL
                     NQSTLSISSESLTLGTEFLFRSSFIPQFDLASSQGLLSRIVYFRRDGNRVLMMESTQG
                     HLASQSLPASMILASLPILTENENRVIVDFNQGMAKVFLAGGWHVSDWIGRHYDPGTR
                     SQAALVEHSFIKDSFTREDTIQTHQIVQLNIGDTYFPSFEARYYLSPYQPNSNFYSKE
                     NLGFRNFGFFETPPQLEPGTGRNNVFITRFSLPENSENKIKYFISANTPPEYVEAVRE
                     GILYWNKAFGKDVLQAELAPMGMTAPDAKMNMVQWIDWDRAGMAYADALSDPRTGEIL
                     NTQVYLTSVFAFSSRQQLRKILRDVRGLLEQTSDKSGDSSVFENQSTLSQLKNRRLAD
                     NGHGKHKHLCHMKVNHQLINALEEIAEHETDDAALLRLSQDYVRDVVAHEVGHTLGLR
                     HNFLGKTALNVSLEERDQSFMNYLTKGELPAESLVVTSSVMDYLNLKDSVISGTRIVR
                     NLPAGEHDRLALQWGYAPSKEKEPVIDQKTVPLFGTDSHLLSHYDVDLFTSGAQPIVD
                     AVREQKRLLAGVANDILERFIAAKAPHHPRDAVDLELLALSTFSHLYNTAVNMLRAVP
                     WMQNDTRALAVERRFPYVSQVNFEAVRVAQWQWVADQIEKAGGVEAAFFPHNARLREG
                     KTILDAETLLKQLDHRLEILRKNGFVGADGKLHRFTENEETLIRDRARSYIDHLNIAL
                     TDLMVDVFLNYKIDYEAVSKNGYLSTDDLSVTFEKKLAEMAEAILTLEAKHTPDGKGV
                     LIGEHKLSNGHTPKILVHRYVYPFSLRLKAAKLLSEDRGHSQAWSLDGRRNILRKLVD
                     SLSRSVGADWNEVKVNDLTPPVRDWFLEQSKLIQTIQTGFGCDDIIKSGSHKDKNEEK
                     QTGLK"
     gene            4195..>5358
                     /locus_tag="PLH57_02340"
     CDS             4195..>5358
                     /locus_tag="PLH57_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01479.1"
                     /translation="MKLKRPWMVLFVFASLWSGGIRSQVDAATIESVTLLAPEQFKNG
                     RATFSDSNLLESERVVLIGVHAKQNSSSGYSYEIRAEGFSGERANLRENDEVDEPDSP
                     DEEGEIILPEVKRHSDFWSDAWSAMTEEKNLNPGVGDPSFQYGRTECKPPYNEGQECE
                     FSILDYEDNFKKETASVRLVTDSFVVFVENRSRVSISNKELRKLIAGFEKRRFAETEK
                     VFGKSTDLDDNGKIFILFSGHLYNSSAREGVMGYVAPWDFIKYRGYPHVGGEIFYLGT
                     PDVYVDAGFSRKNLFKYQYPSTLVHELKHMIAMGRRTSTKKPFEELWLEEPSAVAAEE
                     LVNLGTRLRGPQSSSRSQLKKPQDFAIRYSRRPENADENYGMYGMNFLFLWRQA"
BASE COUNT         1380 a         1205 c         1342 g         1431 t
ORIGIN      
        1 aaactctgtc tgctgttgaa tgtttacgaa ggccgcctca atgttgacct cggaataaag
       61 ttgccaacgc caggttcaac tcggccggtt cctacgaccg aactcgaaaa accagaatgg
      121 accggtcgcc cttgaattcc agacgcatcg tacattcttg tacttccgaa agtttttcag
      181 ggctcgaacg atatgtgctt gatctcgcgt tggaacaata tcgaaccggt ttgaatgtag
      241 aactattttg tcgtgaagga acagagctat ttgaaagtgc ccacgaacgt ggtttgccaa
      301 tttggtcgct taattcgaaa accaaaaagg gtccagcggc ttgggccgaa attcgaaggg
      361 agtggcatcg cagattgtcg gcgggagata tggttctaca catgcatgcc ggcggtgagc
      421 cgtggtttca cctcccgtgg ttattaacgc gtccaaacac cttaaaaaaa accattttgc
      481 agtatcacat ttggatcaat cactcgaaga aagacccgtt acattgggct gtgttccgcg
      541 gaatcgatga agtctggtgt tcttcggaaa ccgcgcgtgt tcatttgagt tcgctgcttc
      601 ctgtgccccg cgatcacttc cgggtggtat tttatggtcg tgacgtcgcg agactttcag
      661 acccttccca aataacttgg cggcgagaaa ttcgtgaacg tttctcgatt cccgaaaacg
      721 aaattcttgg tgtctgtgtc tctcgtatcg aacccatcaa aggcattgcg gagctttttg
      781 atgccttcgt gaactatgcc agtcgtcata aaaccggtac cttattactc gtaggtgaca
      841 cttcgcccaa cgacgaaagc gctgagcgac taggttccga actaaagtcg cgccacacgc
      901 gacttcccga aaatattcga agtcgacttc tgatgcctgg ttacctgggt gaaatcgaaa
      961 aagtcttagc ggcgagtgat ttttatgtcg tgcccgccta tgaggaatgc aattctctct
     1021 caatgataga ttcggcagta atggggaaag ccatattggg taccaattcc ggaggtactc
     1081 ccagtgtcgt tcgtcccggt gaaacaggtg agcttgtgcc acctaaagac gcagcggcta
     1141 tggagcgagg tttggaaaaa ctatttctcg attcagacct acgcacccgc tgcggccttg
     1201 gagcaaaggc tctggcccct actttcgatc gccgccgtat ttttcaagaa atacagcggg
     1261 cctacgaaca ataggctcta cttgagacct gtctgtttct cttcgttttt atctttatga
     1321 gatcccgact tgatgatgtc gtcgcagcca aaacctgttt ggattgtttg gatcagtttt
     1381 gattgttcta gaaaccaatc tcgtacgggt ggtgtcaaat cgttgacttt cacttcgttc
     1441 caatcagctc cgaccgatcg agaaagggag tccaccagtt ttctcaatat atttcggcga
     1501 ccatccaagg accaagcttg gctatgaccc cggtcttcgc tcaaaagttt tgcggccttt
     1561 aagcgcagcg agaaagggta cacgtagcga tgaactaaaa ttttcggggt gtggccgttg
     1621 gagagtttat gttcgccaat gagcacgcct ttgccgtcag gagtgtgttt tgcttcaagg
     1681 gtcagaattg cttctgccat ttccgctaat ttcttttcga atgtaacgga caagtcgtcg
     1741 gtcgagaggt agccattttt cgaaactgct tcgtagtcga ttttataatt tagaaaaacg
     1801 tctaccatca ggtcagtgag agctatgttt aggtggtcga tataacttcg agctcgatct
     1861 cgaatgagtg tttcttcatt ttcggtgaag cggtgtaatt tgccgtcagc acctacgaag
     1921 ccattttttc tcaaaatctc tagtcgatga tcgagttgct ttaaaagagt ctcggcatcg
     1981 agaatagttt ttccttccct gagtcgagcg ttgtgcggaa agaaggcagc ttctacgcca
     2041 ccggcttttt cgatttgatc agctacccat tgccactggg ccacgcgtac tgcctcgaaa
     2101 ttcacctgcg atacgtaagg aaaacgtcgt tcaaccgcaa gcgctcgcgt gtcgttctgc
     2161 atccatggga ccgcgcgcaa catgttaacg gctgtattgt acaaatgaga aaaggtactg
     2221 agtgcaagta attcaagatc aaccgcatcg cgtgggtggt gcggtgcttt cgcggcgatg
     2281 aatcgctcta aaatatcgtt cgcgacgcct gccaacagcc tcttttgttc acgaactgca
     2341 tctacaatgg gctgagcgcc cgaagtgaac aagtccacgt cgtagtggga aagtaaatgg
     2401 gaatcggttc caaaaagcgg caccgtcttt tggtcaatta caggttcctt ttccttagaa
     2461 ggagcgtaac cccactggag tgccaggcgg tcatgttcac ccgctggaag attacgtaca
     2521 atgcgtgtgc ccgaaatcac ggagtctttt aaattcaagt aatccatcac agaacttgtt
     2581 accaccagcg actcagcagg tagctcgccc ttcgttaaat aattcataaa cgattggtct
     2641 ctctcctcga gcgaaacgtt gagggcggtt ttgccgagaa aattgtgtcg caacccgagc
     2701 gtgtgaccga cttcatgcgc caccacgtcg cggacatagt cttgggacaa tcgtagtaaa
     2761 gccgcgtcgt cagtttcgtg ttctgcgatc tcctcgagtg cattgatcag ctggtgattg
     2821 accttcatgt ggcaaaggtg tttgtgtttg ccgtggccat tgtccgcaag cctgcgattt
     2881 ttcaattgtg atagagttga ttgattttca aaaacagatg aatccccgct cttgtccgaa
     2941 gtttgctcta gtaagccgcg aacatcccgc aaaatttttc gcagttgctg acggctagaa
     3001 aaagcaaaaa cgctcgttag gtagacttgg gtattaagaa tttcacccgt gcgagggtcc
     3061 gagagagcgt cagcgtatgc cattcctgcg cgatcccaat cgatccattg aaccatgttc
     3121 attttggcat cgggagcggt cattcccatt ggagccaatt ccgcctgaag aacgtctttt
     3181 ccaaatgctt tgttccaata caaaatacct tctcgaacag cctcgacata ttccggcggt
     3241 gtattggcgc taataaaata cttaattttg ttttcggaat tttccggaag cgaaaatcgc
     3301 gtaataaaca cgttattacg acccgtgcca ggttctaatt gcggcggcgt ttcgaaaaag
     3361 ccgaaatttc taaaaccgag attttctttt gagtagaaat tagagtttgg ttgataaggc
     3421 gaaaggtagt agcgcgcttc aaagctagga aaataggtgt cgccgatatt cagttgaaca
     3481 atctggtgag tttgaatggt gtcttcacgt gtgaaggagt ccttaatgaa gctgtgttcg
     3541 actaatgcgg cttgtgatct ggtgcccgga tcataatgtc ggccaatcca atcactgaca
     3601 tgccagccgc ctgccagaaa aactttcgcc atgccctggt tgaagtcgac gataacgcga
     3661 ttctcatttt cagttagaat tggaagtgag gcgagaatca tcgacgcagg tagcgactga
     3721 gaagcaagat gaccttgggt ggattccatc attagtacgc ggttgccgtc gcgtcgaaaa
     3781 taaacgatgc gactgagcaa tccttggctc gacgccaaat caaattgcgg aataaaagac
     3841 gatctaaata gaaattcagt tccgagagtg agcgattctg aagatatcga caatgtcgat
     3901 tgattcaatt ctccaatcca aagctgatta gggggttggg tggagtccgc gctggcaaaa
     3961 gactcaagtg aactcaaaga aattgaaaga aaaaataaaa ccgacaccac cgctaagcgg
     4021 ccaaagattt tttgcattaa ttacccccaa aaagtcgaac taaggttcgt cggtatcaat
     4081 cagaaatgcg agcgtcaagt tagccgaatg acgtgttgcg tcttcgaaga agtagttttt
     4141 cgagtttacg gtgaacgaaa atggagtaac ctagaaggtt acgaggagat ttttatgaaa
     4201 ttaaagcgac catggatggt gcttttcgta tttgcttctt tgtggagtgg tggaattcgt
     4261 agtcaagttg acgcggctac aatagagagc gttactttgc ttgcgcccga gcagtttaaa
     4321 aatgggcgag cgacattcag cgactcgaat ctgctggaaa gcgaacgagt tgttctaatt
     4381 ggcgtgcatg ccaaacaaaa ctcgagttct ggttattctt atgaaatacg cgccgaaggt
     4441 ttttccggag agcgcgcaaa cttgcgcgaa aacgacgaag tagatgaacc cgatagcccc
     4501 gacgaggagg gggaaataat tcttccggag gtgaagcgtc attcggactt ttggtctgac
     4561 gcttggtctg ccatgaccga agaaaaaaat cttaaccccg gagtcggcga cccttcattt
     4621 cagtacggac gcactgaatg taagcctccg tacaatgagg ggcaggagtg tgagttttcg
     4681 attcttgatt acgaagataa tttcaagaaa gaaaccgcca gtgttcgatt agtcactgat
     4741 tccttcgtgg tgttcgtgga aaaccgaagt cgcgtatcaa tttccaataa ggagctacgc
     4801 aaactgatag ccggttttga aaagcgacgt ttcgcagaga ctgaaaaggt atttgggaaa
     4861 tcgaccgacc ttgacgacaa cggtaaaatt tttattttgt tttcgggtca tctctataat
     4921 tcgagcgcgc gagaaggggt tatgggctac gtcgctccgt gggattttat taaataccga
     4981 ggttatcctc acgtaggagg tgaaattttt tatttaggaa ctccggacgt gtacgtagac
     5041 gccgggtttt cacgcaagaa tctttttaaa tatcagtacc cctctacttt agtgcacgaa
     5101 ttgaaacaca tgattgccat ggggcgccgg acgtctacaa aaaagccatt tgaggaactc
     5161 tggctggaag aaccttcggc ggtggctgcc gaggaactgg taaatctggg aacgcgactt
     5221 cgggggccgc aaagtagttc taggtcccag ttaaagaagc cccaggattt tgcaatccgt
     5281 tattcaaggc gtcccgaaaa tgccgatgaa aattacggta tgtacggaat gaacttttta
     5341 tttctgtggc gacaagct
//
LOCUS       DAOMXO010000066         5336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8179_length_5336_cov_3.988639, whole genome shotgun sequence.
ACCESSION   DAOMXO010000066 DAOMXO010000000
VERSION     DAOMXO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_5336_cov_3.988639"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..2058)
                     /locus_tag="PLH57_02345"
     CDS             complement(25..2058)
                     /locus_tag="PLH57_02345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRK01480.1"
                     /translation="MKFTFLAVLASLLVAAPVVSRANESQQGPFRKIKLSEAPWTADH
                     EKFLRTVLDVFASHPMTQRFLDDQFMGDIRDGLDAEVTHKLQSLPSYGVIRAEAERYL
                     GELIKKTTLVYSGAFAKASGLQHADIMAMVERVSEKLDFSPAARKDAVVFVANTPEIN
                     AYTFSTGKMIRFVVLQGLIQSFNHSAEVLAKNAGESKTREQIAADALEAVIGHELGHI
                     RGEHVLNQMMLVSVLQASIEEQIADMEQQARPETAEVVAALRASMKATLSEQLARAWH
                     HSHSGSVKMSEPFHARLKQGDMGIDLFDASQKLSTFIRKRVKKDLLRSSSQSLFPLEA
                     VEVPQANAASPEAINNMIEQIKAYLEKYGRSCEVTSDRLGQISTSTTAAENADLLLVG
                     AASREAALKVAEEISIQVSEDPTLMEQLLNGTHPLPIYRIVQYRFFENDPIHKIYTTP
                     YLKALHLYLAVTKYMEEVLQRSQDNNKLELPESFLIASTLGKYQTLETLLAQMIVEEL
                     WKDVVDFVSGSNTRGVETIEETLKYLGYREDRQRFVARSPLNAGIASRTGFPKGMFGM
                     LAARLEALDSTAQQLNATTEVSQESTESGTEADATASLIVERVERIKRALALVLSYAP
                     VSRVTQGRAMLSHELGGSGPRRKDNTKGTGPSAAKSPVDDAGAGGCKIVLGED"
     gene            2174..2863
                     /locus_tag="PLH57_02350"
     CDS             2174..2863
                     /locus_tag="PLH57_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01481.1"
                     /translation="MRTNKPSYLIIFSILASLPVLTGVNGCPTSTDSHSLNPAGEFYV
                     EGTSEVTLSTQYRSETLNTQTVLPSYWPSVDAIRAATTMNVRVAERLTDQTVVTLNQL
                     SGEIVRIPLDKTGRGSGLLVSQKERVSDGCFLITSEQVHVQFNEAGDEFELSYSTNWY
                     YDSVVTEHVPCPLYLENYAANLMVIGSPDQYANLRDRLFVSTQALSLERMPELLNLKL
                     THHYVGSRVSE"
     gene            2863..3918
                     /locus_tag="PLH57_02355"
     CDS             2863..3918
                     /locus_tag="PLH57_02355"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRK01482.1"
                     /translation="MLKWVNRGVSKRSLILSGLLLSLVVLGNHSYALSPTYKQAAASQ
                     LIQHLRPELRGVLGQLRNRGILGYCESLLAGTSDPEHRRATLALEALDERFWMAAAAH
                     VPKGEALPEQAIFRLDHHGATYVATNYLIHADYLAGELARAARLPNANSRIPRKRIDL
                     GLALWNSFRDALVTNPRMWRWKLLQRQPSGALLVQYETPWRKPVPSAVALLERPDGSR
                     LALHRTADDREYPNFWSLPGGKVDGIENGFDTVQREVFEETGVLVEHFVRVGRQESSL
                     PSRNGYRYDIEMFYARVPQDIKIRHSTEHDELRWVTPHEVRALHPIGPVTKKLLLGPR
                     PPSTMSSQLNTARHQSE"
     gene            complement(3836..4219)
                     /locus_tag="PLH57_02360"
     CDS             complement(3836..4219)
                     /locus_tag="PLH57_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRK01483.1"
                     /translation="MRWILVIGSIFGFLSVALGAFGAHALRTRLEPRMLEVFETAVRY
                     QMFHALALVAVGLAAATGKTKELYLMGPAVCWVLGVLVFGGSLYALVLTGIKVFGAIT
                     PIGGVLYLAGWTWLTVVAVRAAISS"
     gene            complement(4219..>5336)
                     /locus_tag="PLH57_02365"
     CDS             complement(4219..>5336)
                     /locus_tag="PLH57_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01484.1"
                     /translation="GLVGDLKNPKIYGAGLLSSLSESFHCFDRAVKKLPFDLNCIQQH
                     YDITKPQPQLFVTPNFEALGVVLEQFARTMAFKTGGITGLQKAQRSRAVCTVELDSGV
                     QIGGKLVETLCARDGSPAYLRFEGPCQLAIREKELKGHGVARHPQGFGCPVGPIESER
                     KLSTGKTEITFVSGVKILGVPKPGARGFVKTFTDCKVQWGDRILFEPAWGEYDWVRGN
                     TIKSVFGHAPDRSSYVASMGGYTQRPSKPKTNLTKENAELNVLYAEVRAFRESVLRKK
                     ASLANLPEATGVRLDRVVEKLDRKYPRDWLLRLELLEIAGYLDAKASNGHRVQSWIGN
                     INKRLDEIARGSVEKAEMIARGRELLADASALSKGAA"
BASE COUNT         1299 a         1486 c         1265 g         1286 t
ORIGIN      
        1 gttattgtcc tggaaagcct aagcttaatc ttctcccaaa acgattttgc atccgccagc
       61 ccctgcgtcg tcaaccggac tttttgcggc acttgggcca gtacccttgg tattgtcctt
      121 acgtctaggt ccactgccgc ctagttcgtg gcttaacatc gctcgcccct gagtgactcg
      181 cgatacgggg gcgtaactga gaaccaacgc cagggcgcgt ttaatacgtt cgacccgctc
      241 gacaatcaaa ctagcggtcg catccgcttc ggtgccggat tcggtcgatt cctgagatac
      301 ttccgtcgtc gcattgagtt gctgcgcggt tgagtctaac gcctcaaggc gcgccgctaa
      361 cataccaaac atgccctttg gaaagccggt acgactagcg attcccgcat tcaacggtga
      421 gcgcgctacg aatcgctgac gatcttcccg atagcccaaa tattttaaag tctcttcgat
      481 ggtctcaacg ccgcgagtgt tgctccccga aacaaaatcg accacatctt tccaaagctc
      541 ctcgacgatc atctgagcca atagggtttc gagcgtctga tatttgccca gcgtcgaagc
      601 aattaaaaac gactcaggta actctaactt attgttgtct tgcgaacgct gcagaacttc
      661 ttccatgtac ttcgtgaccg caagatacaa gtgcaatgct ttaagatatg gggtcgtata
      721 aatcttatga atcgggtcgt tttcgaaaaa acgatactgc acgattcgat aaatcggaag
      781 cggatgggtt ccattgagca actgctccat cagtgtggga tcttcagaca cctgaatcga
      841 aatttcttcg gccactttca aagcggcttc gcgactagcc gcgcctacca agagaaggtc
      901 cgcattttct gctgccgtcg tcgaagttga gatctgacca aggcgatcag aggtcacctc
      961 gcaagagcgt ccgtattttt cgaggtaagc cttgatttgc tcgatcatgt tgttgatcgc
     1021 ctcgggcgag gccgcgttag cctgcggcac ttctacggcc tctagtggaa acaaactttg
     1081 ggaactagag cgcagcaagt cttttttaac tctcttgcga ataaacgttg aaagtttctg
     1141 actagcgtcg aagagatcga ttcccatgtc gccctgtttt aaacgagcat ggaaaggttc
     1201 actcatttta acggaaccag agtgggaatg gtgccaagcg cgggccagct gttccgaaag
     1261 agtcgctttc atcgatgcgc gcaaagcagc caccacctcc gcggtttccg gacgtgcctg
     1321 ctgttccatg tcggcgattt gttcttcaat cgaagcttgc agcacgctga cgagcatcat
     1381 ttgattgagt acgtgttcgc cacgaatgtg cccgagttcg tgtccgatca ccgcctccag
     1441 tgcgtcggcc gcaatctgct cgcgagtttt gctttcacca gcgtttttcg ccaacacttc
     1501 ggccgaatgg ttgaaggatt gaattaatcc ctggagaact acaaagcgga tcatcttccc
     1561 ggtgctaaag gtataagcat taatctccgg ggtattggcc acaaatacga ctgcgtcctt
     1621 gcgagctgcc ggcgaaaagt cgagtttttc ggaaacacgc tcaaccatgg ccatgatgtc
     1681 agcgtgttga agccccgagg ccttcgcaaa ggcacccgaa tacaccaatg tggttttttt
     1741 gatgagttca cctaaataac gttcggcctc ggcccgaatc actccgtagc tggggagaga
     1801 ctgaagcttg tgcgtcactt cagcgtccag gccatcacga atgtctccca tgaactggtc
     1861 gtccaaaaaa cgctgggtca ttggatgcga cgcaaatacg tcgagcacgg tcctcaagaa
     1921 cttctcgtga tcggcagtcc agggggcctc ggagagctta attttgcgaa acggtccctg
     1981 ctgagactcg ttggctcggg aaacaaccgg agctgccact agtagagacg ccaaaactgc
     2041 caaaaatgtg aatttcatga gttccctttg ttaaaaatgt cctctggttc cctagcaccc
     2101 cgagaatact tgaacaagaa cttctaaaat ggtagcggtg cgtcaactat tccatgaaag
     2161 gaagtaccaa aatatgcgca ccaacaaacc gtcctatttg atcatttttt cgattttagc
     2221 ttcactaccc gtattgaccg gcgtgaacgg gtgtccgaca agcaccgata gccactccct
     2281 caatcctgcc ggtgagtttt acgtcgaagg tacttccgaa gtgaccctta gcacccaata
     2341 ccgtagcgaa acactcaata ctcaaacggt attaccgagc tactggccca gcgtcgatgc
     2401 gattagagcc gctaccacca tgaatgtacg agtggccgaa cgtttgacag atcaaacagt
     2461 tgttaccctg aatcagctct cgggcgaaat cgttcgcatt cctctagata aaaccggccg
     2521 cggcagcgga ctccttgttt cccaaaaaga gcgagtttcc gacggctgtt tcttgatcac
     2581 tagcgaacaa gttcacgtgc aatttaacga agccggcgat gagttcgaac tttcgtattc
     2641 tacgaattgg tactacgaca gcgtcgtaac cgagcacgtc ccgtgccctc tttatctcga
     2701 aaactacgct gctaacttaa tggtcattgg gtcccccgac cagtacgcca atctacggga
     2761 ccgcttattt gtttctaccc aggctcttag tcttgaaagg atgccagaac tcctcaactt
     2821 gaagttaaca catcactacg ttggttctcg tgttagcgaa taatgttgaa gtgggtcaat
     2881 agaggcgttt cgaagaggtc actgatactc agcggtttgc tgttgagtct tgtggtcttg
     2941 ggaaatcact cgtatgccct ttctccaacc tacaaacaag cggcggcctc ccaactaatt
     3001 caacatttgc gccccgagct gcggggagtt ttaggacaac tgcgcaaccg gggaattctc
     3061 gggtattgcg agagcctctt ggcgggaacc tcggacccgg aacatcgccg cgcgacgctc
     3121 gcattagagg ccttagacga acggttctgg atggctgctg cagctcacgt acccaagggt
     3181 gaagcgctgc ccgagcaggc gatttttcgc ctggaccacc atggggccac ttacgttgct
     3241 accaattacc ttattcacgc cgattacctc gcgggagagt tggctcgcgc tgcgcgcctg
     3301 ccaaatgcga atagtcgcat tccacgaaaa cgaatagatc tgggtttagc gctttggaat
     3361 tcttttcgtg atgcactggt tacaaacccc cgcatgtggc gctggaaact tttgcaacgc
     3421 caacccagtg gagccctatt ggtccaatac gagacgccct ggagaaaacc cgttccttcg
     3481 gcggtggccc ttcttgagcg ccccgacggt tcgcgccttg cacttcatcg tacagccgat
     3541 gaccgagaat acccgaactt ttggtcactg ccgggcggaa aagtagacgg aattgaaaac
     3601 ggattcgaca ccgttcaacg agaggtgttt gaagaaaccg gagtcctagt cgaacatttt
     3661 gtacgagtcg gaagacaaga atcgtctctg ccgagtcgca acggataccg ctacgacatc
     3721 gaaatgttct acgcccgcgt gcctcaagat attaaaattc gccattcaac ggagcacgac
     3781 gaattgcgct gggtcacacc ccacgaggtt cgcgcacttc atccgatcgg tcccgtcacg
     3841 aagaaattgc tgctcggacc gcgaccaccg tcaaccatgt ccagccagct aaatacagca
     3901 cgccaccaat cggagtaatg gcaccgaaaa ctttaatccc ggtgagcacc aacgcgtaaa
     3961 gactgccacc aaacaccagc acgcccaaaa cccagcatac ggctggcccc atcaagtaca
     4021 attctttagt tttacccgtg gccgcggcaa gaccaacagc gactaacgcc aaagcgtgaa
     4081 acatttggta ccgtaccgcc gtttcgaaca cttccaacat tctaggctca agccgcgttc
     4141 gcagagcgtg tgctccaaag gccccgaggg ctacagacaa aaatccaaaa atactcccga
     4201 tcaccaatat ccaacgcatt acgccgcgcc cttagacagc gcgcttgcgt ctgcaagcag
     4261 ttctcgaccc cgcgcaatca tttctgcttt ttctaccgac ccgcgggcga tttcgtcgag
     4321 tcgtttgtta atgttaccga tccaagactg cacccgatgt ccgttagagg ctttcgcgtc
     4381 tagataccct gcaatctcca gaagttccaa ccgcaacaac caatctcggg gatatttgcg
     4441 atcaagtttt tctactactc ggtcgagtct cacgcccgta gcttcaggaa ggtttgccag
     4501 gcttgctttc tttcttaata ccgactcccg aaatgctcta acttcagcgt acaaaacatt
     4561 aagctctgcg ttttccttcg taagattagt tttgggtttc gacggacgct gggtataacc
     4621 tcccatcgac gcaacgtagg acgaacgatc cggtgcgtga ccgaatacgc ttttaatcgt
     4681 attgcctcgc acccagtcgt attcgcccca agccggctca aacagtatgc ggtcgcccca
     4741 ctgcacttta cagtcagtga aggtcttcac gaaaccgcga gcccctggct ttggtactcc
     4801 gagaattttt actcctgaaa caaaagtaat ttcggttttt cccgtagata gtttgcgttc
     4861 actttcgata ggtccaaccg ggcaaccaaa cccttgaggg tgccgggcga cgccgtggcc
     4921 tttaagttct ttttcacgaa tagcaagttg gcaggggcct tcaaaacgca aataggctgg
     4981 cgacccatcg cgcgcgcaaa gggtttctac taattttccg ccgatttgca cgcctgaatc
     5041 aagctctact gtacacacgg cccgcgaacg ttgagctttc tgcaaacctg taattccacc
     5101 cgttttaaaa gccatagtgc gagcgaactg ctccaacacg actcccagcg cctcgaaatt
     5161 aggtgttacg aacaactgcg gttgcggttt ggtaatatcg taatgttgct gaatacaatt
     5221 aaggtcaaac ggaagctttt ttaccgctcg atcgaaacaa tgaaaagact ccgaaagcga
     5281 agacaacaac cccgccccat aaatttttgg attttttaaa tcacctacta aaccgt
//
LOCUS       DAOMXO010000067         5307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8232_length_5307_cov_4.860434, whole genome shotgun sequence.
ACCESSION   DAOMXO010000067 DAOMXO010000000
VERSION     DAOMXO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8232_length_5307_cov_4.860434"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..838
                     /locus_tag="PLH57_02370"
     CDS             125..838
                     /locus_tag="PLH57_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01485.1"
                     /translation="MTGRRVWALGLALSVALLGIFFFESESSSPPATAEVAFSTHNAQ
                     RAPAAETLAPTEATQFETSGWAPVVVPAPDNARLLAEPNASSDSQVAALHTLLQSAIF
                     ESADLETLAHIARTTSQNKTRYLAIEAIARAADARAEAILIGLFEDLDDPTYSQETRS
                     QILSSIEPSDASSSGSRFLLTLFFRADLPDSLKEQALQALVAVSISDESAIEVLVQQL
                     PQTWAERFILATRAIRGQS"
     gene            835..2451
                     /locus_tag="PLH57_02375"
     CDS             835..2451
                     /locus_tag="PLH57_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01486.1"
                     /translation="MTTIKRYLRAATGLCFGVFSIFMTVPAKAEHSVVDYGEAVYCIR
                     GDGAQCTPQMIASPAHRKTLLLPTGYTASEREVFRQDFYRLVSNLALAGTSIYAGDYA
                     DRMVYVGFWIASPPLESGQATFSARIVPHPIRSSALTMDQSKVHKLIESLRKLEPQLF
                     TPWAAIVIYNTDRTDVTANASPPSFIQKPYGLARVTRGDILESPYIPVHELAHAALNF
                     VDEYTEPGLENMSIRSIDRLTPLAVLSGGWSGWVRAVRNLFGVYSYRISEVLAANGND
                     NVDVTKHPTRVATPGFRAETYEYEGGMFFGRGTYHDAGQNLMNDDRANQGADDGFAYA
                     HSPAQQRVIRTAFRFPYRAGRPNDRIRAAGPRGSWRAEWGNTTQLMLFDADKHHRFQP
                     TVRYDVQIGWYDRDWKTCWGGGVVPYPCYENKWVTFQKSQYPSARNIDLNHTALFKVA
                     KLFQKLACHLGIDSIKPNSASQGKYDLCSQDLDSVTASFLPTMVFPMPYQDVSIPAKQ
                     WLTKYHWRFRTYNGSYHSGWTGWSSFTRAL"
     gene            2541..3671
                     /locus_tag="PLH57_02380"
                     /pseudo
     CDS             2541..3671
                     /locus_tag="PLH57_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(3686..4303)
                     /locus_tag="PLH57_02385"
     CDS             complement(3686..4303)
                     /locus_tag="PLH57_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HRK01487.1"
                     /translation="MTPNWDPFWVDSSIVNPPRPMGTREGIGDRLRAAAFAELQAIHG
                     FRWAADRFTQEVSAELRLDWLRLAQEEEKHMNWLLARLEGLGIPLRERPVSDFLWRSF
                     LTCTSARAFCHYIAGAEERGRDAGLRFAKRMQQFDPISAEIFGQIAEEEKSHIELAYR
                     YFPLSTRPFDLANQTPMRAAATAVSPTDSPIQAPNDSSFALPKHR"
     gene            4441..4938
                     /locus_tag="PLH57_02390"
     CDS             4441..4938
                     /locus_tag="PLH57_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01488.1"
                     /translation="MFDSIRLKSFLVFALVHAGFITLGVSLHKLSGTTPANPAMTWAI
                     IGLAVAYFGFVLGSAFLYLPFVPWIKRYHFLANWREWIWAELPTLLALIPLILALGKV
                     AQNAWAELKRHHRRDELDLQKFAKIVSHFADQANGVLQEEEPSASRANKPRKPKSTVR
                     HRKAA"
     gene            5042..>5307
                     /locus_tag="PLH57_02395"
     CDS             5042..>5307
                     /locus_tag="PLH57_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01489.1"
                     /translation="MGIAFVSPDLLNAGDCHDTAEPTVVLCKLTVPSHAQPTWIYLRN
                     DALVENNSRELAPILHFHGIHTSEQSLAGFEFERHLAALSVDTVL"
BASE COUNT         1138 a         1460 c         1306 g         1403 t
ORIGIN      
        1 catatcctgc aaacccggta aaagaaccga tgtagcggtg tttttcccac gggtgaagag
       61 ctatcgggag tttcgaaaga gttttcagtt tacagctttt ttttaaaggc acacaatata
      121 agctgtgaca ggacgtcgcg tttgggccct tgggctcgca ctatcggttg ctctccttgg
      181 tattttcttc ttcgaatcag agtcttcgtc cccccctgca accgcggagg tggcgttttc
      241 tactcacaac gcgcaacgag cgccggcggc cgaaactctt gcgccgactg aggccacgca
      301 atttgaaacc agtggttggg ctccagtggt tgttcccgcg cccgacaacg cccgtctgct
      361 tgccgagcct aacgcttcta gtgattccca agtcgcggct cttcacacct tactacagag
      421 tgcgattttc gagtctgccg atctggaaac acttgcccac attgcccgca ctacgagtca
      481 gaataaaact cggtacctgg cgatagaagc cattgctcgc gccgcagatg ctcgcgcaga
      541 agcaatacta attggccttt tcgaagacct cgacgaccca acttactctc aggaaacgcg
      601 ctctcaaata ctaagcagca ttgagccaag cgatgctagt tccagcggaa gtcgtttttt
      661 acttactctt tttttccggg ccgacttacc agattcactc aaggaacaag cccttcaggc
      721 actcgtcgca gtttcaattt ctgacgaatc cgcgatcgaa gtcttggtcc agcaacttcc
      781 ccagacttgg gccgaacgct ttattctggc gactcgggcg attcgaggac aatcatgaca
      841 actatcaaac gttatttgcg cgccgctacg ggcctttgtt tcggtgtatt ttcgattttt
      901 atgaccgttc cggcaaaggc tgaacactcg gtggttgatt acggtgaagc cgtgtactgc
      961 attcgaggcg atggcgctca gtgtactcct caaatgatcg ccagtccggc gcatcgtaaa
     1021 actctgttac ttccgacggg ctataccgca agcgaacgtg aggtttttcg ccaagatttt
     1081 taccggcttg ttagcaacct tgcccttgcc ggcacttcga tctacgcggg cgactatgcc
     1141 gatcgcatgg tatatgtggg tttttggatt gcaagccctc cactggaaag tggccaagct
     1201 actttttcag ctcgcattgt tccccacccg attcgaagca gcgccttaac aatggaccaa
     1261 agcaaggtcc acaagttaat cgaaagtctc agaaaactgg aaccgcagtt attcactcct
     1321 tgggccgcaa tcgtcattta caacaccgat cgaacggacg tgacggccaa cgcctctccg
     1381 ccctctttta tccaaaaacc ctacggcctc gcacgcgtca cacggggaga cattttagag
     1441 agtccctata taccggttca cgagctcgca catgccgctc tgaattttgt ggacgagtac
     1501 accgaacctg gacttgagaa catgtcgatt cgctcgattg accgtttaac tccgctcgcc
     1561 gtgttatcgg gcggctggtc gggctgggta cgagctgtgc gaaatctctt tggggtgtac
     1621 tcctaccgaa tttcagaggt actcgcggcc aacggcaacg acaatgtcga cgtcaccaaa
     1681 caccccactc gggtcgcgac cccggggttt cgcgccgaaa cctatgagta cgaaggcgga
     1741 atgttttttg gccgcggcac ttaccacgac gcgggtcaaa atttaatgaa cgacgaccga
     1801 gcaaaccaag gagccgacga cggattcgcc tacgctcatt cgccagctca gcaacgggtt
     1861 attcgtacgg cctttcgatt tccttaccgg gccgggcgcc ccaatgatcg cattcgagct
     1921 gccggacctc gcggtagttg gagagcggaa tggggcaata cgacacaact catgttgttc
     1981 gacgctgata aacaccaccg ttttcaacct accgttcgtt acgacgtaca aatcggttgg
     2041 tacgaccgcg actggaaaac ttgttggggc ggcggcgtag tgccctaccc gtgttacgaa
     2101 aataaatggg tcacttttca gaaaagccaa tatccttcgg ctcgcaatat agatttaaat
     2161 cacactgcac ttttcaaggt tgcaaagcta tttcaaaaac tggcctgcca ccttggaatc
     2221 gactccatca aacccaactc tgcgagccag gggaaatacg atctctgttc acaagatctc
     2281 gactcggtca ctgccagttt tctgcccacc atggtgtttc cgatgcctta ccaggacgta
     2341 tccattccag ccaaacagtg gctcaccaaa taccactgga ggtttcgcac ctacaacggc
     2401 tcataccatt ccggctggac tggttggagc tccttcacga gagcattata atagtgtaag
     2461 gttcgtccca caccatgttc agtcgtagat ttttccttga aacacttcgt actctgcgtc
     2521 tcgctgcgcc gatttccggc atgttcctgg ccaccatgct catgcaaata gttgatacct
     2581 ttttcgttgg taaattaggg gccgatgcgt tggggggact atcgataggg gtggctgttt
     2641 tttctttttt catggttacc gccatgggtt tattgttggg gctagattat ttagtgtcga
     2701 aggcctacgg cgagcgtgat ctaaaggaat gccgcaatct tctggtgcaa gggcttattc
     2761 ttgcgacgac tgtttcgctg cccttgatcg ggcttatgca tttggcgcca ctgttttttc
     2821 gccccttcgg actatcggat gtcattagcg caaatgccac cgcgttttta attccacttt
     2881 cttggagcct gcttcccttt ctactcacca atgcttttcg aatgtatttg cagggccagg
     2941 gcaacgcggc ggcggctttc tacgtagtag tgttggcgaa tgttctaaac tttatactaa
     3001 atccggtact catcttcggg gcctttgggt gggctggcat gggtatagcc ggttctggtg
     3061 ttgccacatt tattggtcgt tggttttcgc tttttttaat cgtgggtctt acttactttc
     3121 acactcgctt gccgctctct gcttttgccg ttaccagagc ccagaccatt gccactcaga
     3181 aaaaattact gaagttgggg ctacccgcgg cggggcagct tttgttggaa gttggggtgt
     3241 tcgtggctac gaccttttcg attggtctca ttaatgctca acacctcgcg gcccaccaaa
     3301 tagttcttca catcgcgagt tttacgttca tgatcccgct cggcatttcg gccgcagggg
     3361 ccgtgcgcgt cgggcaggcc gttggacgtc gccactttta ccgagttgcg accgtgggca
     3421 atggagcgct gtttttaagt tttatgatca tggccagctt tggcttagcc ctcagtctct
     3481 tcggcgattc ggtattacgg atattttccc acgaggaacc agtgattgcg attgggaaaa
     3541 aactattgat agtcgctgct ctttttcaga tctccgacgg atttcaggtc accgctgcgg
     3601 gcgtattgcg tggcattggc aataccaagg cgtccctttg ggctaacttc gtagggcact
     3661 ggtggttcgg tttccctctt ggactttacc tgtgttttgg cagtgcaaag ctcgaatcgt
     3721 ttggcgcctg gattgggcta tcagttgggc taaccgcagt ggctgcggcc ctcatggggg
     3781 tttggttggc caaatcgaac ggtcgcgtac tcaaaggaaa ataacggtag gccaactcaa
     3841 tgtgcgattt ttcttcttca gcaatctgcc cgaagatttc agcacttatt ggatcaaatt
     3901 gttgcatgcg tttagcaaat cgtaaccccg cgtctcgccc ccgttcttcg gctcccgcaa
     3961 tgtagtggca aaatgcgcga gcgctggtgc aagtaagaaa gcttcgccac agaaaatcac
     4021 tcaccggtcg ttcgcgcagt ggaatgccga gcccttcgag ccgagccagc aaccaattca
     4081 tgtgtttttc ttcttcttgc gccaaacgta gccaatccaa gcgtagttcc gcgctgactt
     4141 cctgggtaaa tcgatcggcc gcccatcgaa acccgtgaat ggcctgcagt tccgcgaacg
     4201 ctgcggcacg caaccgatcg cctataccct cgcgcgttcc catggggcgc ggaggattta
     4261 cgatgcttga atcgacccaa aatgggtccc aatttggtgt catgcggaga ggggtatcat
     4321 atggcgtacg caaaatgtta ggcagttttt tcctactctt ttggtttttg aagcgactcc
     4381 gaccccttac ccctttcgta gcgagctcta gcgaccgata aaatcactgg aggctcgctt
     4441 atgttcgact caattcgcct aaaaagtttt ttagtgtttg ctctggtcca cgcaggattc
     4501 attaccctag gggtttcgct acataaactc tctggaacca ctcccgcgaa cccagccatg
     4561 acatgggcga ttatcggact ggcggtcgcg tactttggct ttgttctggg ctcggcgttc
     4621 ttatacttac cgttcgtccc ttggatcaaa cggtaccact ttcttgcgaa ttggcgtgaa
     4681 tggatttggg ccgagctccc aactcttctg gccttgatcc cactaattct tgcgctcggt
     4741 aaagtggcgc aaaacgcatg ggccgaacta aaacgccacc atcggcgcga cgaactagac
     4801 ctacaaaaat ttgcaaaaat cgtgtcccat ttcgcggacc aagcaaacgg agtgttacaa
     4861 gaagaagaac cttctgcttc gcgagccaat aaacctcgca aaccaaaatc gaccgtccga
     4921 caccgcaagg cggcctaacc ggctttctct gcgcggctcg ctccccggtg ccttgacagt
     4981 tcttttcgac tctcctaccc ctaaatgaat gcacgttctg cgaatctttt tggttctcat
     5041 attgggtatc gcattcgttt cccccgattt gctaaacgct ggtgattgcc acgacactgc
     5101 tgaacccacg gttgtgctct gcaaattgac cgtacccagt cacgcgcagc ccacctggat
     5161 ttacttgcgc aacgacgccc ttgttgaaaa caactcccgc gaattggctc cgatactaca
     5221 ctttcacgga attcacacca gtgagcagag tcttgcagga tttgaatttg agcggcacct
     5281 agcggcgctt tctgttgaca ctgtact
//
LOCUS       DAOMXO010000068         5286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8273_length_5286_cov_4.736379, whole genome shotgun sequence.
ACCESSION   DAOMXO010000068 DAOMXO010000000
VERSION     DAOMXO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8273_length_5286_cov_4.736379"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLH57_02400"
     CDS             <1..652
                     /locus_tag="PLH57_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK01490.1"
                     /translation="ISYQTQLGPFARFCQIACRKEEAGEQFVFVIDEVNRGNVSRIFG
                     ELMFLLEYRDQELPLLYSRDPFRIPRNLTIIATMNSADRSIALIDYALRRRFRFIEFQ
                     PRTKVLQSWYKLAKANKEHDQCLAFFEFLNCTLSDPKLKVGHSYFLDATAKVTGLTKE
                     KLKHIWEVSVWPLLQEYFVSMPSRLPEYSFETLWKKASADRNENLPTLGGDQEDVA"
     gene            652..1842
                     /locus_tag="PLH57_02405"
     CDS             652..1842
                     /locus_tag="PLH57_02405"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01491.1"
                     /translation="MEHISISEHAVSKPIRLTQMQFDALAVFEKQLSVISAGKEGFFR
                     IKASSWVGDVCLPGLLVSIAPKVGPLKVLRMVVEGDGEANPQIVESPLRPDSDNIWDF
                     LGTTLISEFYKLMGEGLKFDYEVLQENTAYLRGQMQFMQDFLKNFPVRTGIYNRFSEF
                     TPNNDWNKAILWGLTTLTNFTSAEVSVRLTAAADYLAEVEIVANCPDFEMPDAASSYR
                     RILYLVSLVKRFKPVIAGSVGFRGNGIFVDMNDVFEGFVRARLRRVLGKSSFQIPSKS
                     AVAKQLCKNVGLEPDVVITETPGSILAIGDCKYKYDWDFKNSDVFQILAYLEGYRPVT
                     KGYVFVPISAPGLETSSITLPRNRTLIKIGLPAVQLLQDELWQAVAQFLYVELKGKAL
                     PKAG"
     gene            2985..3281
                     /locus_tag="PLH57_02410"
     CDS             2985..3281
                     /locus_tag="PLH57_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01492.1"
                     /translation="MKSRFLVVACTLAAVSISGCLSPSSLDPNSSAGSNAINSKTLSS
                     KIAASTLDEELKAELSALITRLEADPNYQITSEDLDLLKESALLEDGDLAALGT"
     gene            3327..>5286
                     /locus_tag="PLH57_02415"
     CDS             3327..>5286
                     /locus_tag="PLH57_02415"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01493.1"
                     /translation="MVARWKYVLGSIFSLAGLLGIATTALANSNHNERERGFEVRLVP
                     ANGFAQQSGRTTIINGEVWYKGEKDKNHHGGKTLSKLLQDKKVKFRAFFPDMTTEVTE
                     QVVIDENAHDFRYTTPAFSAQGLNTFIVILSDLSPRSKRLLNAQAKIERRIVEFERHL
                     SQLIAQNAKPKKIELLSSQIAKLRVIASRITAKLALEPETLAETRLPLMVDNHTAGQG
                     YATSVQGKYRFTVVSTPGAATTGESAQLTASVTNMRNAPDSDEDENDSQWIVEYRWQG
                     MHVHDSGPVAIDGGQTVSYTYTAEGLAPGNWDANKFHILLYHFDVNRNRYGGRFGKLG
                     LQVGVAADTIAPQWSVTSPALVEHSYFKTMPLVDATVSDAFGRINPNTLEAKLAGTLL
                     NGQNVDLNALSHLTVQTMGSGQSVKLSGNLNPLLEGDYSFSLRASDYAGLSALPNPYQ
                     VSFSIDTTAPSLALGISDHILTSGNSLTLPITVTDRSPTVVRVSRNGTEVYADDAPVA
                     NVNVPLNEGINLIEVSAVDAAGNTATTVQLLDVVRDSTAPLLSNLSPLDGARINALSF
                     IASGVSNEPLAVISVNGQALTLAADKKSFSGNVFLTSEGNSQLVWQATDPAGNTTTVT
                     NAIEAILRVLNAGLVTIEPTANGQELMIK"
BASE COUNT         1405 a         1213 c         1246 g         1422 t
ORIGIN      
        1 aattagttac caaacgcagt tgggaccctt tgcgcgcttc tgtcaaatag catgtagaaa
       61 agaagaagcg ggtgagcaat ttgtgttcgt gattgatgaa gtcaaccgag gaaatgtaag
      121 tcgaattttt ggcgaactta tgtttctgct tgaatatcgc gatcaagaac ttcctttgct
      181 ctactcacga gatccatttc gcattccgcg aaatcttaca attatcgcca ctatgaatag
      241 tgcggacagg tctattgcgc taattgacta tgcgctgcga cgccgatttc gctttattga
      301 gtttcagcca cgcactaagg tgcttcagtc ctggtacaag ctagccaaag ccaataaaga
      361 acacgaccaa tgtctcgcat tttttgaatt ccttaattgc actctttccg atcctaaact
      421 taaggtgggt cacagctact ttctagacgc gaccgctaaa gtgacaggat taacaaaaga
      481 gaagttgaaa cacatctggg aagtttcggt atggccactg cttcaagagt acttcgtgag
      541 catgccctca cgattaccgg agtactcttt cgaaactctt tggaaaaaag cgagcgctga
      601 tcgcaacgag aatctcccca cgcttggagg cgatcaggag gatgtggcgt agtggaacat
      661 atctcgatca gcgagcacgc tgtatcaaag ccaattaggc ttactcaaat gcagttcgat
      721 gcattggctg tattcgaaaa acaactttcg gtcatttcgg cgggtaaaga ggggtttttc
      781 cgtatcaaag cctcgtcatg ggtcggagat gtttgtttgc cgggtttgct ggtatcgata
      841 gcgcccaaag ttggaccact taaggttctt cggatggtgg ttgaaggtga cggagaggcc
      901 aatcctcaaa tcgtggaatc accgcttcgc cccgattctg acaacatttg ggacttttta
      961 ggcacgacac ttataagtga gttttacaaa ctgatgggcg aaggtcttaa attcgactac
     1021 gaagtattac aggagaatac ggcatatctc agaggccaga tgcaattcat gcaagacttt
     1081 ctaaaaaact ttcccgttag aaccggtatt tataatcgtt tttccgaatt tacgcctaat
     1141 aatgattgga ataaggctat tctctggggt ttaaccacac tcacaaattt tacaagtgca
     1201 gaagtttcag tgcgcctaac agctgcagcc gactatctgg ctgaggtgga gatcgttgca
     1261 aactgtcctg attttgaaat gcctgatgca gctagtagct atagaaggat tttatactta
     1321 gtttcgcttg ttaagagatt taagccggta attgctggtt cggtcggatt tcgcggtaac
     1381 gggattttcg tagacatgaa cgatgtgttc gaaggatttg tccgagcaag gcttagaagg
     1441 gttttaggta aatctagctt tcaaattcct tcgaaatcag ccgttgcaaa acaattatgc
     1501 aaaaatgtag gtcttgagcc tgatgtagtg atcacggaaa ccccgggcag tattttggcc
     1561 attggagact gcaaatataa atacgactgg gacttcaaaa actccgatgt atttcagatt
     1621 ctggcctatc tcgaagggta tcgcccggta accaaaggat atgtctttgt gccgatctcc
     1681 gctcctggtc ttgaaacctc ctctatcaca ttgcctagga ataggacttt gattaaaatt
     1741 gggcttcccg ctgtgcaatt gttacaagac gaattgtggc aagctgttgc tcagttttta
     1801 tatgtggaac tcaagggtaa agcacttccg aaagcgggct agtaaaagtt tgaagcatat
     1861 tgcggaataa gtgcggctgc agccaaccgt gtcgtttagc ttataattga aaatagctgg
     1921 atttcgaggt ttagtcattt tctatgccag agctgccaga agtagcgtta tatgctcgcg
     1981 atcttaattt tgccgttcat ggccaaaacc tcgtagcttg taggtttcca aataaaaaga
     2041 actggggcaa tataattatt ccacgtcctg tcgcgcaaac tattaaatcc ctgataggtt
     2101 taagatttaa tttttcatcg ataggtaaag ccctttactt agaagcgagt caacctaaca
     2161 aatcagtagt cgagtttcgc ctcggaatga ccggacgatt ctttcgatcc ccattcgaca
     2221 ataggtggcg cgctcattgt tttattgagc ttcattttga acggtgctcc atctattact
     2281 gtgatcaccg tcgattcggg cgtgtattta gggatacaga agcgcaaggg ggtattggtg
     2341 gcttttctag caaacgaggt ctttggtttg atcccaatcc gattccgcct actgggtaca
     2401 aatcatcacc gcgaatttcc tggttgataa atacagggca taggactggc gttggcaact
     2461 acatggcaaa tgaggctctt ggacgtctaa atctttctcc ttttcagcca tgctcatcag
     2521 aggaggaagc tgtggccatt cttagggagt gtcagaaagt cgcaagatcg agttttcgat
     2581 tcgggggaaa ttcatttagc tcaggttatt tccgacttga tgggacggag gggcgctttt
     2641 cggggcaatg taagttttat ggccatcctc gagtcccccg atatctattt cgtggacgtc
     2701 cggtcttcac attccaccat cccaagtaag ccaggcaggt acagcaattg gcaatgaagt
     2761 gttgccaagg tctcataagg cagcgcttta ttaagatcat cactatacgg gtcttcatca
     2821 gcacggttgt aagaataacg acgtaaaagc aatttcataa aaacttaact tttcgatata
     2881 agttgctgtt atttaatagt tttaatacat gatcattttg tttttaatta tgccaaatta
     2941 gcttatacta gatgctagtt cacagttttt cgggggttag tttcgtgaag tctagatttc
     3001 ttgtagtcgc atgcactttg gcagcagtta gtatttcggg ttgcctatct ccttcaagtt
     3061 tggacccaaa ctcaagtgcc ggtagcaacg ccattaactc caaaactctt tcatcaaaaa
     3121 tcgcagcttc aacgcttgat gaagagctga aagccgagct ttcagcgctc atcactcgcc
     3181 ttgaggccga tccaaattat caaatcacct ccgaagacct tgatctcctc aaggagtccg
     3241 cgctcctgga agacggagat cttgcagcac tcggcactta gggcaaattt cgcgcacaac
     3301 agtttctgga agtagagggg ttaccgatgg tagcacgttg gaagtacgtc ttgggttcta
     3361 ttttttcgct cgcaggcttg cttggtattg caaccacggc tttggcgaac tcaaaccaca
     3421 atgaacgcga acgtggtttt gaagtgcgct tagtgcccgc caatggcttt gcccaacaat
     3481 cgggccgcac taccattatt aatggtgaag tttggtacaa aggcgaaaag gacaaaaatc
     3541 accacggtgg aaaaacctta tcgaaactgc tgcaagacaa gaaagtaaaa tttcgggcgt
     3601 ttttcccgga catgacgacg gaagtcaccg aacaagtggt gatcgatgaa aacgctcatg
     3661 actttcgtta caccactcca gcatttagcg ctcaaggcct caacactttc atcgtgattt
     3721 taagtgatct ctcaccgcgc tcaaagcgcc tgcttaatgc ccaggccaaa atcgagcgac
     3781 gcattgtgga atttgaacgg cacttgagcc aactcattgc ccaaaatgct aagcctaaaa
     3841 aaattgaact actaagttca cagattgcga aactacgtgt gatcgctagc cgcatcaccg
     3901 caaaattggc gcttgaacca gaaaccctgg ctgaaacccg tttgccgcta atggtagata
     3961 accatacggc aggtcagggc tatgccacga gcgttcaagg taagtaccgt tttacggtag
     4021 tgagcacccc gggcgctgct acgactggtg aaagtgccca gctcacggcg agcgttacga
     4081 atatgcgtaa tgcgccagat agtgatgagg acgaaaacga tagccaatgg attgtcgaat
     4141 accgctggca aggcatgcac gttcacgact caggtccagt agcaatcgat ggtgggcaaa
     4201 cggtttctta cacttatacg gctgagggtc tagctcctgg taactgggac gctaataaat
     4261 ttcatattct gctttatcac ttcgacgtta atcgtaaccg ctacggcggc cgttttggca
     4321 aactgggtct gcaagtgggt gtggcggctg acaccatagc tcctcaatgg agcgtcacaa
     4381 gtccagctct tgtggaacac agttacttta aaaccatgcc gctcgttgat gccaccgtga
     4441 gcgatgcgtt tggtcgcatt aatccaaata ctcttgaagc aaaactggca ggcacacttt
     4501 taaacggcca aaacgtggac cttaacgcgc tttcgcacct aaccgttcaa accatgggct
     4561 ctggtcaaag cgtaaaactt agcggcaatc ttaatccgct tcttgaaggc gattattcgt
     4621 tttccctacg cgcatcggat tatgccgggc tttcggcact gcccaatccc tatcaagtga
     4681 gtttctcgat cgatactacc gcaccaagct tagcgcttgg aataagtgat cacattctca
     4741 ccagcggcaa ctcgctcacg ctgccgatca ctgtgaccga tcgttcacct acggtggttc
     4801 gagtttcacg aaacggcaca gaagtttatg ccgacgacgc accggttgcc aacgtcaacg
     4861 ttccgctaaa tgagggaata aatctcattg aagtgtcggc agtcgatgct gccggtaaca
     4921 cagctaccac agtgcagtta ctcgatgtgg tacgagacag cacggcgccc cttctttcaa
     4981 acttaagccc gcttgacggc gcacgaataa atgctctctc atttattgca agcggcgtat
     5041 cgaacgagcc gcttgcagtt atttcagtaa atggccaagc cttaacgcta gcggctgata
     5101 aaaaatcatt tagtggcaac gtgtttttaa cttccgaggg aaattcgcag ctcgtctggc
     5161 aagctactga ccccgccggt aacaccacca cggttacaaa cgccatcgaa gcgatcttac
     5221 gggtgctaaa cgctggttta gtaaccattg agccaacggc aaacggccag gagcttatga
     5281 ttaaag
//
LOCUS       DAOMXO010000069         5266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8318_length_5266_cov_5.080215, whole genome shotgun sequence.
ACCESSION   DAOMXO010000069 DAOMXO010000000
VERSION     DAOMXO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8318_length_5266_cov_5.080215"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PLH57_02420"
     CDS             complement(<1..457)
                     /locus_tag="PLH57_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01494.1"
                     /translation="MLLHPSGGLLYHLRALRYRKPLWKPFSTQLEPWLSEAFRGCSEL
                     LVVGPSAGYCLSSRFLKQFNKIYFIEPDPIARLLFTGRSLKWPHGATAKSIASTHAIL
                     DPSPMRFLKILDSMPRAGVLFSNILGQLSLYLDDFDEASKRPWVLNPGAI"
     gene            complement(458..1858)
                     /locus_tag="PLH57_02425"
     CDS             complement(458..1858)
                     /locus_tag="PLH57_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRK01495.1"
                     /translation="MFDIAKLVQDNRSRGLDLQATYINPAFAKVLKTIGFDRTWTRGQ
                     GAYLWDDKEDRYLDLLSGYGVFGVGRNHPTVRDALKTAMDADLPSLIKMDAPVLSGLL
                     ARELLKKAAHTECERVFFANSGAEAVEAAMKFSRAYTGRNEFLYLNQSYHGLTLGALS
                     VNGCASFRNGFGELYDARALRMGDLAALEEALATKRYAGFIFEPVQGKGVYFPNDDYY
                     QQAANLCKRHGTLFIADEVQSGMGRTGKFFAHEHWNLKPDLITISKSLSGGYVPISAV
                     LLSKHVHQKTFSSMDRSVVHSSTFSQNDFAAAAGLATLHVLEAENLIQNAERMGQLFL
                     DLCRPMVDRFEMLKEVRGKGLMVGFEFGAPKSLSLNIGWKLVHTADKSLFAQLIVLPL
                     FTKHKILTQVAGHGIDIIKLLPTLTVGEDDVRWFARAFEDVMIELHKFPGSIWDVGLQ
                     LAKGAIGGKPKPEVTI"
     gene            complement(1913..2986)
                     /locus_tag="PLH57_02430"
     CDS             complement(1913..2986)
                     /locus_tag="PLH57_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRK01496.1"
                     /translation="MSNALKKSTGSMTPESTVAANEKLIAAIDKYYANTSQIKSSHCA
                     VPIELALSEEVPDVSLFLATTDSKNHPAYKIFREEGLYLSTVEVQALIDNGVKNVYIL
                     RSDVPKFTQYVERVLVEMPTTSPMVDQKKVTLLRDSAIGVMTEIFASPTPEAIEKGVK
                     VVNGFVYLLMKDPKAYDILLSLSSHDHYTLQHSVGVATNAIILGKKVGVNDEAALIEL
                     GIGGLLHDIGKTKVPTEIINKKGPLDESEWAVMKQHSLFGYEILKDNPNVGMRAKLAV
                     LQHHEETNGTGYPVGLSGPQIDLFAKVTTIADIYNALTTDRSYSTARSPFDAFKLIRD
                     KLSHKIDAELFDSMVKIYGGKAP"
     gene            3378..3725
                     /locus_tag="PLH57_02435"
     CDS             3378..3725
                     /locus_tag="PLH57_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01497.1"
                     /translation="MANRDESQKVAFVYSNIYQLYKKAQAAADTQTKDLPDAKVFRTD
                     ELTPAKVSSFKPLEFKSATAPSAASPSPHGARQKDLVNPFDDLKTNISKLQDLHSRLR
                     FMLKELEDLVEKK"
     gene            complement(3735..>5266)
                     /locus_tag="PLH57_02440"
     CDS             complement(3735..>5266)
                     /locus_tag="PLH57_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01498.1"
                     /translation="DGIGGGDSDTNAIRRRALEEQKARELDRIEDRYKKQVKETQKNR
                     QKALEQVYQATEGEVESKRDDAQFKVDRAKQAADERVRAFEQDALRMTEEAKRQFEHK
                     AKALEKASLQMDNERRYLAKQHSDTMAHIQKEREERYNDLSSKHAREALRIENEYKRR
                     NMTAEQRHASEIAQKEREIKDKKAAIQAEGNRTLDFLQRDKANQAASIENEVNHTRQQ
                     GRAQIEEQRINQATQKMLLDSDHAASIDEQNRRYNLEINRTSMRGKRMLDETREKQMV
                     ETFMAEKDGKEKVRNAQTQAAVKLATIENEGKLKEHLAKQAFSHQQQIVEQNNQQSLN
                     DARVQSEFMRKQAAENLNLQSKQLKEKSEKTLGDQTNQYATDIMFNRKIGQKQLDATV
                     LANAKKVASYSSRAADPFYRVHNLNVSVSDGDQFVTLKVPASPHDEGQVRVTHQPGRL
                     TVSTTRRFEGNAESDDGRKVTTNSYQSYSESVPITGALNMKDLTKTFDGSNVIFKIPR
                     V"
BASE COUNT         1204 a         1223 c         1360 g         1479 t
ORIGIN      
        1 cgatcgcccc tggattcaaa acccacggtc gtttcgaagc ttcgtcgaag tcgtcgagat
       61 aaagcgaaag ttggccgagt atgttcgaaa ataaaactcc ggctcggggc attgaatcga
      121 gaattttcaa gaaccgcata ggtgacgggt cgagaattgc gtgtgtcgat gcgattgatt
      181 tggcagtagc tccgtggggc cactttaggg aacgcccagt aaacaaaagg cgggcaattg
      241 gatcgggttc aataaaatag attttgttaa actgttttaa aaaacgcgac gaaaggcagt
      301 atcccgcact agggccaacc actaagagct cactgcaccc acgaaaagct tccgagagcc
      361 acggttcaag ttgggtggaa aatggcttcc acaggggctt gcgatacctt aatgcacgaa
      421 gatgataaag aagtccgccg gacggatgaa gcagcattta gattgtcacc tctggttttg
      481 gtttcccacc aatggctcct ttagcaagct ggaggccgac gtcccaaata ctaccaggaa
      541 acttatgaag ttcgatcatc acatcttcaa aggcacgggc aaaccagcga acgtcgtctt
      601 cgccaacagt cagagttgga agcaatttga taatatcaat tccatggccc gccacttggg
      661 tgagaatttt gtgtttggta aatagtggca acacaatgag ttgagcgaat aagcttttgt
      721 cagccgtgtg taccagtttc cagccaatgt tgagcgaaag tgatttcggt gcgccgaatt
      781 cgaaacccac catgaggccc ttacctcgaa cttctttcaa catctcgaac cgatcgacca
      841 tcgggcggca tagatcaagg aacaactgcc ccatgcgttc tgcattttga attaggtttt
      901 cagcttctaa tacgtgcaat gttgcgagac ccgctgctgc ggcgaaatcg ttttgactaa
      961 acgtcgaaga gtgcaccaca ctgcgatcca tgcttgagaa agttttctga tgaacgtgtt
     1021 tagacaacaa aacagccgaa atcggaacgt agccgccgct caaactctta gaaatcgtaa
     1081 ttagatctgg ttttaaattc caatgttcgt gagcaaagaa tttaccggta cgacccatac
     1141 cgctctgcac ttcatcggca ataaagaggg tgccatgtcg tttgcagaga tttgcggcct
     1201 gctggtagta atcgtcattc gggaaataga cgcctttgcc ctggacgggt tcgaagatga
     1261 atcctgcgta ccgtttagtg gctagtgctt cttcgagcgc cgcgagatcg cccatgcgca
     1321 acgcgcgcgc gtcgtagagt tcgccgaagc cattgcgaaa acttgcgcat ccattcaccg
     1381 atagcgcgcc gagagtgagg ccatggtaag actggttcaa atacagaaat tcgtttcggc
     1441 cagtgtaggc acgggaaaat ttcatggcag cttccacggc ttcggcgccc gagttggcga
     1501 aaaacacgcg ctcgcattcg gtgtgagcgg ctttctttaa aagttctcgc gcaagcaaac
     1561 ccgataagac gggagcatcc attttaatga ggcttggaag gtcggcgtcc atcgcggttt
     1621 tgagagcatc gcgcacggta gggtgattgc ggccaactcc aaaaacgccg taccccgaaa
     1681 gaaggtctaa gtagcggtct tccttgtcat cccacaggta ggcgccttga cctctagtcc
     1741 aggtccgatc gaacccgatc gttttgagga cctttgcaaa ggcggggtta atataggtgg
     1801 cttgcaggtc gaggccacga gaccgattgt cctgaacgag ttttgcgatg tcgaacatga
     1861 gcgccctctt tatgacagag agcttacgaa atttgttcga ttttggaaac tgttacggag
     1921 ccttgccacc gtagattttg accatcgagt caaacaattc ggcgtcgatt ttgtgagaca
     1981 gcttgtcgcg aatcagtttg aaggcatcga aaggtgaacg ggcagtggaa taagaccggt
     2041 cagtcgtcag tgcgttataa atgtccgcaa tagtggtgac cttggcaaat aggtcgattt
     2101 ggggtcctga tagcccgacg ggatagccag ttccattggt ctcttcatga tgttgtagga
     2161 ccgcaagttt ggcacgcata ccaacgttag gattgtcttt tagaatttcg taaccgaata
     2221 gagagtgttg tttcatgacg gcccactccg attcgtcgag gggtcccttt ttattgatga
     2281 tctcggttgg aactttggtt ttaccgatgt catgcagtag cccaccgatg ccgagttcaa
     2341 ttaaggcggc ttcgtcgttt acgccgactt ttttcccgag aataatggca ttggttgcta
     2401 cgcctacgga atgctgaagg gtgtagtggt cgtgactcga aagagataac aaaatgtcat
     2461 aggctttggg gtctttcatt aaaaggtaaa caaatccgtt tacgactttt acgccttttt
     2521 cgattgcctc cggagtgggt gatgcaaaaa tttcggtcat cacgccgatg gctgagtcac
     2581 gaagaagtgt gacttttttc tgatcaacca tgggcgaagt agtgggcatt tccactagca
     2641 cacgctctac atattgggta aatttcggaa catcgcttcg caggatgtag acgtttttca
     2701 ctccattatc gatgagcgct tgaacctcga ccgtactgag atacaggcct tcctcgcgaa
     2761 aaattttgta ggccgggtgg ttcttgctgt cggtggtggc aagaaagagg gaaacatcgg
     2821 gcacttcttc tgacaatgcg agttcaatgg gcactgcgca gtgcgaactc ttgatctgac
     2881 tagtattggc gtaatattta tcgatcgcgg caataagttt ttcattggca gcaacagtgg
     2941 attcgggcgt catggaaccg gtcgatttct taagtgcgtt gctcattgcc gcaggtctcc
     3001 ttacagtttg ggtgtaggtc ggcacgcgcc aacttccagt ggatctactt agtgatatcg
     3061 acagatcaga gcggtacctt gatcggaaat gtttaccttt cccactagga gcccggctgg
     3121 tccgggctca ttgtcgttaa aaaggggcgc agtgtcacct cgacctaggg gtgtctaaag
     3181 tttagacatt ttcgctaggc aattcttggc gtttcccctg ggttttctta ggggttttct
     3241 cccccattcg tggattcaat cgtggcatcg cgcttgcttt agtataagag cgtgaggctg
     3301 gacgtcaccc accagtcggc acaatgtgga agcgaaagta gtgggtgcat gagtgagagc
     3361 gccagaggag gcagcaaatg gctaaccgtg atgagtccca gaaagtcgca ttcgtctatt
     3421 caaacatcta tcaactctac aaaaaggccc aggctgccgc agacacgcag accaaagatt
     3481 tacccgatgc taaagtattt cggaccgacg agttaacccc ggctaaagtt agtagcttta
     3541 agccattaga atttaaaagc gctactgcgc cgtcggccgc gagtccttcg ccgcatggtg
     3601 cacgccaaaa agacttggta aacccgtttg atgatttaaa aactaacatt tcaaaactgc
     3661 aagaccttca ctcgcgtctg cgattcatgt tgaaggaact cgaagatctc gttgaaaaaa
     3721 agtagttgtg cccgttaaac gcgcggaatc ttgaagatca cgtttgatcc gtcaaacgtt
     3781 ttggtaagat ctttcatgtt cagtgcaccg gtaattggca ctgattcgga atagctttgg
     3841 taggagttag ttgttacttt gcgcccgtcg tctgattcgg cgttcccttc gaaacgacgc
     3901 gtggtgctca cggtgagtct tcctggctga tgagtgactc gcacttggcc ttcgtcgtgt
     3961 ggggaagccg gtactttgag agtaacaaac tgatcaccat cggaaaccga aacattcaga
     4021 ttatgcacgc gatagaacgg gtcggctgcg cgcgatgaat agctcgccac ttttttagcg
     4081 ttggctaaaa ccgttgcatc gagctgcttt tgaccgattt tacgattaaa cataatatcg
     4141 gtggcgtatt gattggtttg gtctccgagg gttttttcgc tcttttcttt gagctgtttg
     4201 ctttgcaagt ttaaattttc agccgcttgt ttccgcataa attccgattg cacgcgagca
     4261 tcgtttagcg actgttggtt gttttgttct acgatctgtt gctggtgact gaaggcctgt
     4321 ttagcaaggt gttcttttaa tttaccctcg ttctcaatag ttgccagttt taccgctgct
     4381 tgggtttgag cattacgaac cttttctttg ccatcttttt cggccatgaa ggtttcgacc
     4441 atctgtttct cgcgcgtttc atcgagcatg cgtttaccgc gcattgaagt acgattaatt
     4501 tctagattgt aacgacgatt ctgttcgtcg atcgacgctg catggtcact atcgagcaac
     4561 attttttgag ttgcctgatt gattctttgt tcttcaattt gagcgcggcc ttgttgccga
     4621 gtgtgattga cttcgttttc aatgcttgcg gcctgattgg ctttgtctcg ctgtaagaaa
     4681 tctagtgttc ggttgccttc ggcctgaata gctgcttttt tgtctttaat ttcccgttct
     4741 ttttgggcga tctcagaggc gtgcctttgc tccgccgtca tattgcggcg tttgtactcg
     4801 ttttcaatgc gcagcgcttc gcgtgcgtgt ttagacgaaa ggtcgttgta ccgttcttcg
     4861 cgctcttttt ggatgtgggc catggtatcg ctgtgctgtt tagcgaggta acggcgttcg
     4921 ttatccattt gtaaggaagc cttttcgaga gcttttgctt tgtgttcaaa ttggcgttta
     4981 gcttcttcag tcattcgaag agcgtcttgc tcgaaagcgc gaacgcgttc gtcggccgct
     5041 tgtttagcgc ggtccacctt aaactgcgcg tcgtcacgct tagactcgac ctcgccctct
     5101 gtcgcttggt acacctgctc aagcgccttt tgacgatttt tttgggtctc tttaacttgt
     5161 tttttgtagc ggtcttcaat gcggtcgagt tcgcgggctt tctgctcttc tagcgcacgt
     5221 ctacgaatcg cattcgtatc ggagtcgccg ccaccaattc cgtcta
//
LOCUS       DAOMXO010000070         5245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8366_length_5245_cov_4.880925, whole genome shotgun sequence.
ACCESSION   DAOMXO010000070 DAOMXO010000000
VERSION     DAOMXO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8366_length_5245_cov_4.880925"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1197
                     /locus_tag="PLH57_02445"
     CDS             112..1197
                     /locus_tag="PLH57_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRK01499.1"
                     /translation="MKSKNLLSTLVLGLFALSVAGGCSKKSGSDETSKTTETAPTARV
                     LQIHTWSNYISPAVIEAFEKKTGAKVSLNFFSSNEELLAKLQAGARGYDIIMPSSYMI
                     QALKELNLIQPLGSLDWPELAKLSKRFQNPSFDPKHEFTIPFAWGTTGIAVNRAKIKN
                     AIDSWDWIFAHPELKGQVTMLDDAPAVVGAALKYLGYPYNEASPEAFAKVKALLMKQK
                     KWLKAYSAEVLPLLESGEVALAQAYSGDVLQARRKNPNIEYVLPKEGAELFVDTMAIP
                     VGAPVPELAKEFMRFTLQIENAATQVKHLFYSPVVDLTGVAGTEELLKNPAINPTPEL
                     DKRLEIMIESAERSEAIQRLWTELKSS"
     gene            1200..2621
                     /locus_tag="PLH57_02450"
     CDS             1200..2621
                     /locus_tag="PLH57_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01500.1"
                     /translation="MTKSNSSDRTSFRIAIALVVVWTLVHLWLASQRSMWQDEIARYD
                     QMRMPVWDAFKSLFSEPSPFAPGELVLGFISKFLFGSWTSFEFWGRLPVVLWGSATLA
                     LAVRQRDPLLVGLLIFSTSLTSFTTQFRPYGALIFGGALAFRMVAIQTPLARWERFLV
                     WFSILFGHLYGICFVGLACFLKRRWWPTVTAALYVVGLLIIYRSVHDGSIVVWKDTHT
                     WNFDFREAAQHTLRAVFNPYRTAFLLLPIAAIGFISTWRRNKTCGLHATIILLVMSIG
                     PVIANYLGKYYFTPRQIVSLIFPALVFSSLGIKNLIHFSRRFKFHKIIKVAISSLVIL
                     VMLRAWTLFVINGRPPFADQPLHRHKHIAERLLKENSDLVVLVDPGGTGYHYFIRALG
                     PPSIDRIVRLGEFEFRSRVWTHGFELFHFDQSDFAWRDLGELAHSEVFRDYLKAHQSR
                     IDGLIYNSEKFHLETSIPSFRAW"
     gene            2837..3370
                     /locus_tag="PLH57_02455"
     CDS             2837..3370
                     /locus_tag="PLH57_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01501.1"
                     /translation="MKKKNFIGLALLLLAVAGLTSGCGGASEEETLPNNGVHGAPNVP
                     VPPQPNGYPNGGGGYQVPAGNYCYWTPGNNQPSCGACPLGYQQSSPGYCTSSGGGNLG
                     CPSGYTVSQWGTCVPQGFNCPVGTNFSWQYGGCVASSPISSGYYSCSRSGYFYVCRYT
                     YNPPYPNHYFNGWGWVR"
     gene            complement(3426..4265)
                     /locus_tag="PLH57_02460"
     CDS             complement(3426..4265)
                     /locus_tag="PLH57_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01502.1"
                     /translation="MEPIPRSRHPYFERLLEFRAHAWDQSSGDIPIGRWAEFFPKSTK
                     KIVYEIGCSNAQFLCEIAKANPHVGFVGLDWKFKVLYRGAQRIAAEQLKNVGLIRGLV
                     QNSNHFFSENEVDEIWIFFPDPWAKVAQLKNRIMNETQLVQLAKLLKPGGRIYFKTDH
                     PGYYQWVHALFGQPLPRVGHYAPLADAQVKAGARSRRIKQVKVRLPAGSLRGDIPPES
                     VLLKRVFEFEYAATLPAEEFGSSSCFTSHKTLFEKVFEKEGLPGYGIQLLRNSESANR
                     IHD"
     gene            complement(4414..>5245)
                     /locus_tag="PLH57_02465"
     CDS             complement(4414..>5245)
                     /locus_tag="PLH57_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRK01503.1"
                     /translation="GFRIATNTQSIAAQRTLGLTNDAQKRSLEKLSAGERIVRAGDDA
                     AGLAISEKLKAEIRSMRQANRNANDGISLVQTAEGGLNEIQNILIRLRELSMQAATDT
                     IGDTERSFTDKEVQNLVNEVQRIADVTQFNGRYLLNGSGELLEFQVGTHNKPEQDRLT
                     YDTSITDVTTGKLGIAGMTVGNKESAQANLDVLDKAINMVSENRSEFGALQNRLQSTI
                     NNLNVADENLSAANSRIRDVDFANESSELTKRNILAQAGTAMLAQANSNGLLALKLLG
                     "
BASE COUNT         1245 a         1308 c         1245 g         1447 t
ORIGIN      
        1 agattcggtg tgactcccga aatcaatgcg atttcttcgg cggtactttt cttaactctc
       61 gctgtggtcg ttcttgtttt caagctgcga aaagagtaaa agttcggtca tatgaaaagc
      121 aaaaatcttc tttcgacatt ggtattgggc ttgttcgcac tttcagtagc cggtggctgt
      181 agcaagaaat caggctcaga cgaaacttcc aaaacaacgg agactgcccc taccgctcgt
      241 gtgctccaga ttcacacttg gtccaactac attagtcccg ctgtaatcga ggctttcgaa
      301 aaaaagactg gcgccaaggt ctcgcttaat tttttctcct caaatgaaga gctcttagca
      361 aaattgcagg cgggcgcccg tgggtatgac ataatcatgc cttcgagcta catgatccag
      421 gctctcaaag aacttaactt aattcagccg ctgggttctc ttgattggcc agaacttgca
      481 aagctttcga aacgatttca aaatccaagt ttcgacccta aacacgaatt cacgattcct
      541 tttgcgtggg gcaccaccgg aattgcagtg aatcgagcga aaattaaaaa cgcgatagat
      601 agctgggatt ggatattcgc ccacccggag ctcaaagggc aggtaacaat gttagacgac
      661 gctcccgctg tggttggggc ggctctaaag tatctcggtt atccctacaa cgaggctagt
      721 cccgaagctt ttgcaaaagt taaggcgttg ttgatgaaac aaaaaaaatg gctcaaagcc
      781 tattccgcag aagttcttcc gttgctcgag tcaggggaag tagccttggc gcaagcctat
      841 tcgggtgacg ttttgcaagc tcgccgtaaa aatccaaata tcgagtatgt acttccaaaa
      901 gaaggcgctg aactctttgt cgacactatg gcgatccccg taggtgcgcc tgtgcctgag
      961 ttagctaagg agttcatgcg gttcacgctt cagattgaaa atgctgcgac acaagtgaag
     1021 catcttttct attcgcctgt ggtagatctc actggtgtgg ccggaaccga agaacttctt
     1081 aaaaatccag ccatcaaccc gacacccgaa ctcgacaaac gactcgaaat catgatcgag
     1141 agcgccgagc gcagtgaagc tattcagcga ctttggacag aactaaaatc ttcgtaacga
     1201 tgaccaagag caactcgtcc gatcgcacgt cttttcggat cgcaattgcc ttggtagtag
     1261 tttggaccct ggttcacttg tggctcgcct ctcaacgatc aatgtggcaa gacgagatcg
     1321 ctcgttacga ccagatgaga atgcccgttt gggatgcctt caagtcccta ttttcagaac
     1381 ctagcccttt cgcccccggc gaactcgttt taggtttcat ttctaaattt ttatttggtt
     1441 cgtggacttc atttgagttt tggggacgat tacctgtcgt gctttggggt tcggctaccc
     1501 ttgcccttgc cgttcgccaa cgggaccctt tattagtcgg actactaatt ttctctacct
     1561 cactaactag ttttactacg cagtttcggc cgtacggtgc attaatattt ggcggcgccc
     1621 tagcttttcg aatggtggca attcaaacgc cgctcgctcg ctgggaacga ttcctagttt
     1681 ggttttctat tctattcgga catttgtacg gaatatgttt cgtaggatta gcgtgctttt
     1741 taaagcggcg ttggtggcca accgtcactg cggcgctata tgtggttgga ttactgatca
     1801 tttatcgtag cgtacatgat ggttctatcg tggtctggaa agacacccac acctggaatt
     1861 ttgattttcg cgaggccgcc caacacacgt tgagagcagt gttcaatccc tatcgcacgg
     1921 cttttttgct tctaccaatt gcagctattg gatttatttc gacttggaga cgaaataaaa
     1981 cttgcggact tcatgccacg attattctat tggtcatgag cattggtccg gtaattgcga
     2041 actatttagg aaagtattat tttactccac gccagatcgt ttcactgata ttcccagcgt
     2101 tagtattttc gtctttagga attaaaaacc tgattcattt ttcgcgtcgg tttaagttcc
     2161 ataaaattat taaagtcgcg attagcagcc tcgttatttt agttatgcta cgagcttgga
     2221 ctcttttcgt gattaacggc cgccctcctt ttgccgatca gcctcttcac cgtcacaaac
     2281 acattgccga acgtttatta aaagaaaatt cagacttggt agttttggtt gatccgggag
     2341 gaacgggata tcactatttt atacgggcac tcggccctcc ctcgatcgac cgaattgtac
     2401 gcttagggga attcgaattt cgatcccgag tatggactca tggttttgag cttttccact
     2461 tcgaccagtc agacttcgct tggcgcgatt tgggcgaatt ggcccactcc gaggtattta
     2521 gagactatct taaagcacat cagagtagaa tcgacggctt aatctataac tctgaaaagt
     2581 ttcacctaga gacatcgatc ccctcatttc gggcgtggta actcgaacac ctgtataaaa
     2641 gttcgtcact cttttttcgt caccttaggt gagggctcct ctcaagcaaa aacccccgtt
     2701 cggctaggtc cttgaactca ctcgcaaaat ttcattggta gcactttggc actattgctg
     2761 cacataggcc cgacgaacac gttgaacggc agagataaag cagaacgtag aactgtaagt
     2821 gtttggagtg atatttatga agaagaaaaa ttttattggt ctagcgcttt tgttgctcgc
     2881 agtggccggc ctcacgtcag gctgcggcgg agcgagcgaa gaagaaacgc ttccgaataa
     2941 cggcgtacac ggcgcaccga acgtgccagt accaccgcaa cctaacggtt acccgaatgg
     3001 cggcggcggc tatcaagttc cagctggtaa ctactgttac tggacacccg gaaacaatca
     3061 accaagctgt ggcgcttgcc ccctcggtta ccaacaatca agccctggct attgtacttc
     3121 tagcggtggc ggtaacctcg gttgcccatc cggctacaca gtatctcagt ggggcacttg
     3181 cgtacctcaa ggattcaact gtccggttgg cactaacttt agctggcaat acggcggttg
     3241 cgtagcaagt tccccaattt cgagtggcta ctactcgtgc tcgagatccg gttacttcta
     3301 cgtgtgtcgc tacacgtaca accctccgta cccgaaccac tacttcaacg gttggggttg
     3361 ggttcgctaa ccagatttga acaagttgtc cctgtccccc tactcctctc tctcaggcga
     3421 aggcgtcaat cgtgaatgcg attggcgctt tcgctgtttc tgagcaactg aatgccgtaa
     3481 ccagggagtc cttctttttc gaataccttt tcgaacagcg ttttatgaga agtgaaacag
     3541 ctgctacttc cgaactcttc agctggtaaa gtagcggcgt actcaaattc gaaaactcgc
     3601 tttaaaagca cgctctcggg aggaatatcc cctcgtaatg accccgccgg taaccgaacc
     3661 ttcacttgct tgatacggcg gctgcgggct ccggccttta cctgggcgtc ggccaagggg
     3721 gcgtagtgcc caacccgggg caatggctgc ccaaagagcg cgtgcaccca ctggtagtaa
     3781 cctggatgat cagttttaaa atagatgcgc ccaccgggct ttagaagttt cgctagctgc
     3841 acgagctgag tctcattcat gatgcggttt ttaagctgcg cgaccttggc ccaggggtcc
     3901 gggaaaaaga tccagatttc atcgacttca ttttcagaaa aaaagtggtt cgaattttga
     3961 actaagccgc gaatgagccc aacgttttta agttgctcgg ccgcaattct ctgagcccct
     4021 cgatagagga ctttgaattt ccagtccagg cccacaaaac caacatgggg gttggccttc
     4081 gcgatttcgc agagaaactg agcattagag cagccaatct cataaactat cttcttggtg
     4141 gattttggaa agaattcggc ccaacgcccg ataggaatgt cgcccgaact ctggtcccac
     4201 gcgtgcgcgc gaaactccag taggcgttcg aaataggggt gccgtgatct tggtatcggt
     4261 tccatacgcc gccttatagc gccaaaaact ctcgggtgcc aggtttcccc ggcacccttg
     4321 atgtttctgt tataaggacg agaagcgtat gagttgggac gaccaatacg ctgctactac
     4381 acgaactact ctgctaaaaa ctcttcctga tattcagccg agaagcttca gtgcgagcag
     4441 accattgcta ttggcttggg ctagcattgc ggtgccagct tgagccaaga tattgcgctt
     4501 ggtcagctcg gaagattcat tcgcgaagtc tacgtcgcga attcggctat tggcagctga
     4561 gaggttctca tctgctacgt tcaagttatt aatggtagac tgcaatcggt tttgaagagc
     4621 gccaaactcc gatcggtttt cactcaccat attgatcgct ttgtcgagca catcgaggtt
     4681 cgcttgagcg gactctttgt tcccaaccgt catgcctgcg ataccgagtt tccccgtcgt
     4741 tacgtcagta atcgacgtat cgtaggtcaa tcgatcttgt tctggtttat tgtgagtacc
     4801 gacttggaac tcaagcaatt cgccactgcc attcaataag taacgaccat tgaattgagt
     4861 tacatctgca atacgttgca cttcgttaac aaggttttga acttccttgt ctgtgaagct
     4921 gcgctctgta tcgccgatcg tgtctgtggc tgcttgcatt gagagttcgc gcaaacggat
     4981 taagatattt tgaatttcgt tcaatccgcc ttcggcagtt tgcacgagag aaataccatc
     5041 gtttgcgttt cggtttgctt gacgcatact gcggatttca gcttttaatt tttctgaaat
     5101 cgcgagtcct gcggcgtcat cgccagcgcg aacgatacgt tcacctgccg acaacttttc
     5161 gagcgagcgt ttttgcgcat cgttcgtcag tcctaatgta cgctgagctg ctatactctg
     5221 cgtatttgtt gctatacgaa aaccc
//
LOCUS       DAOMXO010000071         5232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8405_length_5232_cov_4.935870, whole genome shotgun sequence.
ACCESSION   DAOMXO010000071 DAOMXO010000000
VERSION     DAOMXO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8405_length_5232_cov_4.935870"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            536..1378
                     /locus_tag="PLH57_02470"
     CDS             536..1378
                     /locus_tag="PLH57_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRK01504.1"
                     /translation="MERNNCPVIAVTNQKGGVGKTTTAINLAQALALQDLRVLLVDLD
                     PQGNATQGVGINLETIQGSVAELIRDRALPVERVIYKGDGLDLIPATPLLSRVEREMV
                     SMTNAELRLAQRLQPLRSQYAIIVIDTPPTFGPLMNSALNAASQLIVPVDSSYFALMG
                     IKELLAEVDEIQRGTNPRLSVLGYLLTLVDPTNIAGQTWDTLVLNFGETVFETKIKRS
                     VKLRESPALGKTIFHHAPESSGARDYLGLAQEVMERLGIGEVANLGKDTRPALSVVTG
                     VAHE"
     gene            1371..1994
                     /locus_tag="PLH57_02475"
     CDS             1371..1994
                     /locus_tag="PLH57_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01505.1"
                     /translation="MSKSDLKAAMQSLQVNKPLRLPAKAPSFTPPEAAPLVEKPTQVE
                     NSTLVDSHTEAVKSTVAQPTTQAAIAPEVQKQTEVELPTTVESPSLAQETAVVEKQTS
                     VGNKTEVASSTSPRRATEQDSSVLLEERAIEGLEKGYTRLPNTTLMRLASGDFTKNEI
                     KLALLIARFRFRFSASALRFQSQFSNAVAGFVEPLYLKRFLGFVRKD"
     gene            2045..2764
                     /locus_tag="PLH57_02480"
     CDS             2045..2764
                     /locus_tag="PLH57_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01506.1"
                     /translation="MPPEWDQLINSNRDGNTTLVEKAPEVTKTTLVKKMIPAEVQKST
                     EAGVGNQTYFKDKENIENKLSLSLLPEILSKYFAELKPAKKRESELNYFQNLQNEFPD
                     VDIADCLAFVQKHGVGRGENVQPCHSPMAYLSKAIAEIMPEIEKLRKARYERIAREKR
                     ETESKQMRAAVEEREAAEWTIREEAFTRAFSSEEKQTEVIHKYLQTLPFKPNDRIARM
                     VAIDSWWEELNAREPRSTNSN"
     gene            complement(2761..3417)
                     /locus_tag="PLH57_02485"
     CDS             complement(2761..3417)
                     /locus_tag="PLH57_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01507.1"
                     /translation="MDTTLTTWIKKNAYPNGQIKTAARYQGKSLHGVAVGYFASGGVR
                     FVEHFKKGKLHGNCRHWYENREPHCKGNYVEGRREGFWQWWHSNGKPSNCAVFRKGQK
                     NGWGASFHENGITSGIIHWVDNQADGIAVGWFDNGELEYVSNYKEGVLHGFSLEQDSH
                     GRVVSYNIYENGRLVLGSVPNALEVEKLIKTAIRGVRSNLQISLVQFGRSKLRSRSAA
                     "
     gene            3565..3933
                     /locus_tag="PLH57_02490"
     CDS             3565..3933
                     /locus_tag="PLH57_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRK01508.1"
                     /translation="MKRQKKKKDPPRDDYYNLGARVRFIRESRELSQKSLAKTAGISQ
                     STVAQIETGRMDPSVNTLQKIAVALDTDIATIFASKDIHVFDLARLKSKYGDVEKLTP
                     HLYTALGKIIQYAKDIGFLK"
     gene            3930..4241
                     /locus_tag="PLH57_02495"
     CDS             3930..4241
                     /locus_tag="PLH57_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRK01509.1"
                     /translation="MMNNWGIPLEIETLVRSRDRECVYCHIEFNKAGTGSGASWEHII
                     NDIKITTAENIALCCRSCNSSKGAKSLRVWFSKDYCVKRNINESTVADIVRVHISKYG
                     Y"
     gene            complement(4248..4943)
                     /locus_tag="PLH57_02500"
     CDS             complement(4248..4943)
                     /locus_tag="PLH57_02500"
                     /inference="COORDINATES: protein motif:HMM:NF037895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01510.1"
                     /translation="MAKQRQSRHLVVQYIERVRSEIFEVYHREIKKFAKGRNGIYTLY
                     SGNKLYYVGLASSLTGRINGHLRDRHKGEWDTFSMYLTADPTYLKEIESLILRIVDLK
                     GNRVRGKLKNALDLRRELLKELKYSQKTQLDFLMGRTDYKRLFMATKHIGENQTELIR
                     YGLGSTNICAKYKGKTYQARIDRNGWVLFKSKKFSSLSAAGIHILNRSCNGWTFWMAK
                     SRTGKWEKLDAWR"
     gene            complement(4947..>5232)
                     /locus_tag="PLH57_02505"
     CDS             complement(4947..>5232)
                     /locus_tag="PLH57_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HRK01511.1"
                     /translation="DNNGTVVDPYKLLPPIFSDLDVAEMDLLITSDSIADGGGAMTAY
                     ARMQFTEMSEIECDRVTKALLKYCELDTFAMVLIFEYWKTQIKAFTEKVA"
BASE COUNT         1425 a         1283 c         1155 g         1369 t
ORIGIN      
        1 cggggggaat tgaacagccg gaagccccgg ggtacccgct tgcgggtcgg ggctgaggcc
       61 gggctgcagg ccaagtgcga gcagcacttg gaccggaagc cgactccccg cgggtccacc
      121 aatcttggaa aggctcccac agagaaattt gtgggagcct tttaatttcg ggttgttagg
      181 attcaagcag ttacgagttc acagcagctt cgacatttcc cggtcagtcg cgcgatttcg
      241 ggaatctcct acaaacttcc tatttttttc ctactttgcg gcccgctttc acccgtgtgc
      301 accacggaga aaaggcgggc ctatgtaaac cacattcaaa tcactgaaat aaaaaaagcc
      361 cagagcgatt gctccaggct tttaaacttt gtcgtcatca gctcgcagat cttggcgggt
      421 cgtgaagctg attctttcaa actcaaccgt gaccgcgctc gaagcgcggt ttctggtcgg
      481 tacttctcca ttcttacgga gtcgtgccgt tgctgtcaac tcgaatggag aaatcatgga
      541 acgcaataac tgccctgtca tcgctgttac caaccaaaaa ggcggtgttg gaaagacgac
      601 caccgcaatc aacctcgccc aagccctagc gcttcaagac ctgcgtgttt tactggtcga
      661 tctcgaccct caaggtaacg caactcaagg cgtagggatc aacctcgaaa caattcaggg
      721 ctccgtagct gaactaatcc gcgatcgagc gcttcccgtt gaacgcgtga tttacaaagg
      781 cgatggactt gatctcatcc ccgcaacgcc cctactttct cgtgttgaac gcgaaatggt
      841 ttcgatgacg aatgccgaat tacgcctcgc acaacgactt caaccgttgc gttcgcagta
      901 tgcaattatt gtcatcgaca ccccaccgac gttcggccca ctaatgaact ctgctctgaa
      961 tgcggcatcc cagctcatag tgccagtgga ttccagctac ttcgcactta tgggcattaa
     1021 ggaactgttg gcggaagtgg acgaaattca acgcggcacc aacccgagac tttcggtgtt
     1081 gggctatttg ctcacgcttg tcgatccgac gaatatcgcc ggtcaaacct gggatacgct
     1141 tgttttaaac ttcggtgaaa cagtcttcga gaccaagatt aaacgctccg taaaacttcg
     1201 agaatctcct gcacttggca aaacgatctt tcatcatgct cccgaaagct ctggtgctcg
     1261 cgactatcta gggcttgctc aagaagtaat ggaaaggctt gggatcggtg aagtcgctaa
     1321 ccttggcaaa gacacgcgcc cagccctctc tgttgtaacg ggggtggcac atgagtaagt
     1381 cagatctaaa agcagcaatg caatcgctcc aagtcaataa acctctgcgt ttacctgcga
     1441 aagctccgtc gtttacaccg ccagaggcag cacccctggt cgaaaaacct acccaggtgg
     1501 aaaattcgac cttggttgat tctcacaccg aggccgtaaa atcgaccgtg gcacaaccaa
     1561 caacccaggc agcaatcgca cccgaggtcc aaaaacaaac cgaggttgaa ttacctacca
     1621 cggtcgaaag tccttccttg gctcaagaaa ctgccgtggt cgaaaaacaa acctcggtcg
     1681 gaaacaagac cgaggtcgcc tcatcaacca gcccccgtcg agcaacagag caggacagct
     1741 cggtgctact cgaagagcgt gcaatcgagg gattggaaaa aggctacact cgactaccaa
     1801 atacaactct gatgcgtctc gcaagcggtg actttaccaa gaacgagatc aagctcgcgc
     1861 tcctcattgc gcgctttcga tttcgtttca gcgcaagcgc gctccgcttt caaagtcagt
     1921 tctcgaacgc cgtagcgggc ttcgtggagc cgctgtactt gaagcgcttt ctgggctttg
     1981 tcagaaagga ctaatcgaga aaattcaggg tgatgaaaat cgcccgaatc aactgggcct
     2041 tgttttgccg ccagaatggg atcagctcat caattcgaat cgagatggca atacaaccct
     2101 ggttgaaaaa gcgcccgagg taacaaaaac gaccttggtt aaaaaaatga tccccgccga
     2161 ggttcaaaaa tcaaccgagg cgggggtcgg aaatcagacc tactttaaag ataaagaaaa
     2221 tatagaaaac aaactctctc tttccctgct tccagaaatt ttgagtaaat attttgctga
     2281 actaaagccc gcaaaaaagc gagagagtga attgaattat tttcagaacc ttcaaaacga
     2341 atttcccgat gtagacatcg ctgattgcct cgcatttgtt cagaaacacg gtgttggtcg
     2401 cggtgaaaat gtacagcctt gccactcgcc gatggcctac ctatcaaagg ctatcgctga
     2461 gataatgcct gaaatcgaaa agctgcggaa agcgcgttat gaacgtatcg cacgtgagaa
     2521 acgagaaaca gaatccaaac aaatgagagc tgcagtggag gagagagagg cagccgaatg
     2581 gacgatccgt gaagaagcct tcacaagagc gttttcgagt gaggaaaaac aaacggaagt
     2641 aattcacaag tatttgcaaa cgcttccgtt caagcccaac gatcgaatcg cacgaatggt
     2701 ggcgatcgat tcctggtggg aagaacttaa cgcccgagag cccagaagca cgaactcaaa
     2761 ttaagccgcg cttctacttc gaagtttcga ccgaccaaac tgaaccaaag agatttggag
     2821 gttgctcctt actccccgaa tcgcagtttt aatcagtttt tcaacttcga gtgcatttgg
     2881 aacagagcct aataccaatc ggccgttttc atatatgttg taggatacaa cacgcccatg
     2941 ggagtcttgc tcaagggaaa agccatgcaa tacaccctct ttgtaatttg aaacgtattc
     3001 gagttcgcca ttatcaaacc aacctacggc tattccgtcg gcttggttgt cgacccaatg
     3061 tatgattcca gaggttattc cattctcatg aaacgacgct ccccacccat ttttttgccc
     3121 ttttctaaat actgcgcagt ttgatggttt gccgttggag tgccaccact gccaaaaacc
     3181 ttcccggcgt ccttcgacat aattgccttt gcaatgaggc tctctatttt cataccaatg
     3241 gcggcaattc ccatggagtt ttcccttttt gaagtgttct acaaatcgca cacctcccga
     3301 agcaaaatat cctactgcca ctccgtgaag cgatttccct tggtatctgg ccgctgtttt
     3361 gatctggcca tttgggtagg cattcttttt gatccaggtc gttaatgtcg tatccatatg
     3421 aaactcctat agatttactt aattgtaatg ccttgcgtta atttagtcaa gtattattac
     3481 taatatttga tattgttaac gaattctttg atatactcat actacttatt aagtagtgat
     3541 aataatcata ggatatcaac caaaatgaag cgacaaaaga agaaaaaaga cccgccacga
     3601 gacgactatt acaacctcgg ggctagggtt agatttatac gcgagtctcg cgaactatcg
     3661 caaaagtcgc tcgctaaaac tgcgggcatt tcgcagtcta cagttgccca gatcgaaact
     3721 ggacgaatgg acccaagtgt aaatactctt caaaaaattg cggtagctct tgataccgac
     3781 attgctacga ttttcgcgag taaagatatt cacgtattcg acctcgctcg ccttaaatcg
     3841 aaatacggcg atgtcgaaaa actaacgcct catttgtaca cggcattggg aaaaataatt
     3901 caatacgcaa aagatatagg ttttttgaaa taatgaataa ctggggtatc ccgctagaaa
     3961 ttgaaacttt agttcgaagc cgagatcgcg aatgcgtcta ctgccatatc gaattcaaca
     4021 aagccggcac aggtagcggt gcttcatggg aacatattat aaacgatata aaaattacga
     4081 ctgctgaaaa tattgctctt tgctgtaggt catgtaattc gagtaaggga gcaaaaagtt
     4141 tgcgagtttg gttttctaaa gactattgtg tgaaacgcaa tataaatgag agcactgtag
     4201 cagatattgt gcgggtccac atttcgaaat atgggtatta aacttcccta tcgccacgca
     4261 tcgagctttt cccatttccc ggttctgctt tttgccatcc aaaatgtcca accattacaa
     4321 cttcgattta gaatgtgaat tcctgctgcg cttagtgaag aaaacttttt actcttaaac
     4381 aaaacccatc cattgcggtc aatgcgagct tgataagttt tccctttgta tttagcacag
     4441 atattcgttg acccgaggcc atatctaatg agctccgttt gattttcgcc gatgtgcttt
     4501 gttgccatga ataagcgctt gtaatctgta cgccccatta agaaatcgag ttgtgttttc
     4561 tggctatatt tcaactcttt aagaagctcg cgccgcaaat ctagtgcgtt ttttagtttg
     4621 cccctaactc tgttgccttt tagatctact atcctcagga taagagattc aatttccttt
     4681 aaataggttg gatcagcggt gagatacata ctaaatgtgt cccactctcc cttgtgacgg
     4741 tctcgaaggt gaccattgat tcttccggtt aagctcgatg caaggcccac atagtatagt
     4801 ttgttgcctg agtagagagt ataaatgccg tttcggccct tggcgaattt ctttatttcc
     4861 cgatgatata cttcaaatat ctcacttcga acccgttcta tgtactgaac gacgaggtgt
     4921 cgcgactgtc tttgctttgc cattttttac gccactttct ctgtaaatgc ttttatctgt
     4981 gttttccaat actcaaaaat tagcaccatt gcgaacgtat ctagttcaca atatttcaaa
     5041 agtgctttcg ttactctgtc acactcaatt tctgacattt cggtgaattg cattcttgcg
     5101 tatgcggtca ttgcgccgcc gccatctgca atgctgtcag aggtaatgag taaatccatt
     5161 tctgctacat caagatcaga aaaaatcggt ggtaatagtt tatacgggtc tactacagtg
     5221 ccgttgttat cc
//
LOCUS       DAOMXO010000072         5216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8446_length_5216_cov_4.235032, whole genome shotgun sequence.
ACCESSION   DAOMXO010000072 DAOMXO010000000
VERSION     DAOMXO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8446_length_5216_cov_4.235032"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLH57_02510"
     CDS             complement(<1..408)
                     /locus_tag="PLH57_02510"
                     /inference="COORDINATES: protein motif:HMM:NF020118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HRK01512.1"
                     /translation="MNLSDLSRVIEQVGKDRGIKKEIIIEALEQAVLLAAQKKFGEGA
                     QLESHFNNDSGEIELFQFKQVVESDDDIADPDLEIALDEARKLDSDVSVGDELGIKLD
                     NPAFGRIDAQTAKQVIFQKVRDAERDIIFGEFIH"
     gene            complement(558..995)
                     /locus_tag="PLH57_02515"
     CDS             complement(558..995)
                     /locus_tag="PLH57_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01513.1"
                     /translation="MTEIKANSKAALEQLEESVRAIIEPLGYDVVALEQTSGPARSGR
                     TLVLYIDFARNADAHTAEYIGLDDCAKVNAAVDELFETTALLDGTYTLEVSSPGVERP
                     LRRAMDYARFSGQKARLHTFRPLEADEIGNEGYWKKKQKQKTS"
     gene            complement(1165..3468)
                     /locus_tag="PLH57_02520"
     CDS             complement(1165..3468)
                     /locus_tag="PLH57_02520"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRK01514.1"
                     /translation="MVRFVRAQRAIILFLLLTFMHSVLAGASERTVSGGKWMPHQMDA
                     HAEKLKELGLEIDTKELSNLDLGLLRAIVSLGFCSGSFISPNGLIITNHHCAQALLSF
                     MSQIDRERGIQSDYVTNGFHAKTLAEERSAGPAQKIYVTQKIVEVSEAILRDIDFIQD
                     PHEKRMTIERRMAEMVKAAELNRPGIRVEVKEFYRSSQFFLIEQREIQDIRVVYAPPK
                     MVGFFGGDADNWHYPRHTGDWAILRAYVAPDGSSAPYDEKNVPFQPAQFLKIAPNGLS
                     PNDLVMVAGYPGITNRLLTTAEVTRQMNESMPDTITYFRMIIETYDNLSKQGEDLRIK
                     VESSNFGAANVLKKNVEQHEALTREGFLKKKLDQERDFQSWIAAEPNRMLKYGWASKV
                     LESIYEKYKNELKDALANRLFARSPLLASALTIVRMADERAKPDEEREPAYKQRNWPR
                     IIQSEIAKQGTIDKRIDTAIFNAVMAYTDKTVPLNERPKYVEQLFAQWHADPKFAEQL
                     YANTALDNQETRVKLLETATLEELEQSSDKAIQLALRILPIIKAQDRLARSYAGEASK
                     PMRRLMEALQMYHQIKGDPLAPDANSTLRLTYGIAKGYFSPTRQRYMPPFTNARGILD
                     KNRPGSAEFEVPHQLVRALNIAYFDPERKIDTGSGFGPLGDPMFGDLPVNFLATVDTT
                     GGNSGSAAMNKRGELIGLLFDGNEDSLAVDFRFDDKGDRSILLHIAYALWYMSKVDKA
                     YNLLEEMTIPAANCRDYLLEGSAKPSK"
     gene            3634..4611
                     /locus_tag="PLH57_02525"
     CDS             3634..4611
                     /locus_tag="PLH57_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01515.1"
                     /translation="MKFLHLLRTALVLSAFSVYSLQPTWAAEIKPDCNQVVQTSGVRG
                     KLSRWWKLLKAPFGKSRSEDRRFSIDVLNAEISDLPSEVQKRILVDLLPQLRPYSGED
                     LVEVLQKVRDETRGYFYLNLRRGITKKIDSVTQEKLRMLLSREDRRAAVEILNDRWMV
                     DDLDLPKVELTEANLEPQGDGRIVLWKLWDQLEADKLGLYDARELLLLLEQVPVGHAR
                     GFNDHGLPRGVERRTGRLPYDQFLAIMGDFAQKTGVRLELKSIPMDLLVTAVILDRLA
                     HLPNASDVQKQRAFEMAVLIFRESRHWEKISQPEQWNWLVFFRRLGHID"
     gene            complement(4608..>5216)
                     /locus_tag="PLH57_02530"
     CDS             complement(4608..>5216)
                     /locus_tag="PLH57_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRK01516.1"
                     /translation="KRAAFAAETYWGKPVPSFGDPAARLFLLGLAPAAHGANRTGRMF
                     TGDNSGLWLYRALYKAGFASQPESRNAADGLQLRDAWISATVHCAPPENKPTLKEIEN
                     CAPNLMAEFEYLSRVRVILALGAIAYKEAIKTLRAGPLGIDPKTKIPKFSHGVCTQLP
                     HGRWLLGSYHPSQQNTFTKRLTEPMFDEIFKTARRLLDGQAL"
BASE COUNT         1238 a         1265 c         1336 g         1377 t
ORIGIN      
        1 gtgaatgaac tccccgaaaa tgatgtcgcg ctctgcatcg cgcacttttt gaaaaattac
       61 ttgtttagcg gtttgcgcat caatgcgtcc aaaagccgga ttatccagct tgattccgag
      121 ttcgtcacca acgcttacgt ccgagtcgag ctttcgcgct tcatcgagtg cgatttctaa
      181 atctgggtcc gcaatgtcgt cgtcagactc tacgacttgt ttaaactgaa ataattcgat
      241 ttctccgctg tcattgttga agtgcgattc aagctgggcg ccttcgccga acttcttctg
      301 cgctgctagc aatacggcct gttcaagcgc ttcgatgata atctcttttt tgataccgcg
      361 gtctttacca acttgttcaa tcacccgact gagatctgat aagttcatcg tttttccctt
      421 ttaacctttt ctaaaccctt gaggtgccac gtattcgagg tgagccttcg aaatcatttg
      481 cagcggtaca gccacgaggg ttccgtctat ttccatttgg accttgtttt ggtcttcact
      541 tagacccttc aaaatcccta cgaagttttt tgtttctgtt tcttcttcca gtatccttca
      601 ttgccgattt catccgcttc cagcgggcga aacgtatgca gccgggcctt ttggccgcta
      661 aaacgggcat aatccattgc gcgtctaagc gggcgttcta caccggggct tgaaacctca
      721 agtgtataag tcccatcgag caacgccgta gtttcaaaca gttcatcgac tgcggcgttt
      781 actttggcac agtcgtcgag accaatgtac tcggcggtgt gcgcgtcagc gttacgagca
      841 aaatcaatgt agagcaccaa ggtgcgaccc gaacgagccg ggccagaggt ttgctcaagc
      901 gcgaccacgt cgtatccaag gggttcaatg atcgcccgca cactctcttc gagctgctct
      961 agcgctgcct tagaattcgc tttgatctcg gtcatttcaa ctcacgacaa aaaaaaagcg
     1021 ggcataagag cgacggtcct tgcccgccac tccctggccc acttctaaaa gtagtccaac
     1081 tgttggcgtt tctttagcac ggcgcttgtg cgaaaggcca gcaaaaaaga ctgaaaagtc
     1141 gcaggactag ccctggaaat tgctttattt cgagggcttg gcactgcctt ccaggagata
     1201 atcccggcaa ttggccgccg gaatcgtcat ttcttcgagc aggttatagg ctttgtcgac
     1261 tttggacatg taccagagcg cgtaagcgat gtggagcagg attgatcggt cgcctttgtc
     1321 atcgaatcga aagtccactg ccagcgaatc ttcgttgccg tcgaaaagca aaccaatgag
     1381 ctcgccccgc ttattcatgg cagccgatcc tgagttaccc ccagtggtgt cgacggttgc
     1441 cagaaaattc accggcaaat cgccaaacat agggtcgccc aatgggccaa atccactgcc
     1501 ggtgtcgatt ttccgttccg gatcgaaata cgcaatattg agagcgcgaa cgagttgatg
     1561 cggtacttcg aattcggctg atcccgggcg gtttttgtct aatataccgc gggcgttggt
     1621 gaaaggaggc atgtatcgct ggcgggtcgg actgaaatac ccttttgcga tgccgtaggt
     1681 tagccgcaga gtagagttcg cgtcgggtgc cagggggtcc ccttttattt ggtgatacat
     1741 ttggagtgct tccatgaggc ggcgcatcgg tttcgaggct tcgccggcat aggaacgagc
     1801 caagcgatct tgagccttga tgatcggcaa tattcgcaac gccaattgaa tcgccttgtc
     1861 tgaggattgc tccagttctt ccaaggtggc ggtttcaagc aacttcacgc gggtctcttg
     1921 attgtcgagc gccgtgtttg cataaagctg ttcggcgaac tttgggtcgg catgccattg
     1981 ggcgaaaagt tgttccacgt atttgggccg ctcgttgagg gggacggttt tgtctgtgta
     2041 ggccatgact gcattaaaaa tggcagtatc gatacgttta tcaatggtac cttgtttggc
     2101 aatttcactt tgtatgatgc gaggccagtt gcgttgttta taggccggct cgcgttcttc
     2161 gtctggtttg gctctttcgt cggccattcg gacaatggtc agggccgagg ccagaagagg
     2221 cgaccgggca aagagccgat tggccaacgc gtctttgagt tcgtttttat atttttcgta
     2281 aatgctctcc agcaccttcg atgcccagcc gtatttcagc attcggttcg gttcggcggc
     2341 gatccaactt tgaaaatcac gttcctggtc taatttcttc ttcagaaagc cctcgcgggt
     2401 aagagcttcg tgttgctcta cgttcttttt taaaacgttc gcagcaccaa aattcgaact
     2461 ttctacctta atacgcagat cctcaccctg tttggagaga ttgtcgtagg tttcaataat
     2521 cattcgaaag tatgtaatag tgtccggcat tgactcattc atttggcgag tcacttcggc
     2581 agtcgttaaa agtcgattgg ttattcccgg ataacccgcc accatgacga gatcgttagg
     2641 acttaaacca ttgggagcaa tctttaagaa ttgcgctggc tgaaagggga catttttttc
     2701 gtcgtaaggg gctgaagaac cgtcgggcgc gacataggcc cgcaaaatgg cccaatcgcc
     2761 agtgtggcga gggtaatgcc aattgtcagc gtcaccacca aaaaaaccga ccatcttcgg
     2821 aggggcgtac accacgcgaa tgtcttgaat ttcgcgctgc tcaattaaga agaattgtga
     2881 agagcgatag aattctttta cctcaacgcg aatgccgggg cgatttagtt cggcggcttt
     2941 aaccatttcc gccatacgtc gttcaatggt catccgtttt tcgtgtgggt cttgaataaa
     3001 atcgatgtca cgaagaatgg cctcagaaac ttccacaatt ttttgagtaa cgtaaatctt
     3061 ctgcgccggt ccggccgagc gttcttcggc aagagttttt gcatgaaacc cattggttac
     3121 atagtcggat tgaatgccgc gctcacggtc tatttgagac atgaaggaga gtagcgcctg
     3181 tgcacagtga tggttggtga tgatgagacc gttgggggaa ataaaagatc cggaacaaaa
     3241 acccaggcta acaatggccc gcaacaaacc gagatctaag ttcgaaagtt ctttagtgtc
     3301 gatttcaaga cctagttcct ttagtttctc ggcatgggcg tccatttgat gaggcatcca
     3361 tttgccaccg gaaaccgtac gttcgctggc cccggccaag acgctgtgca tgaacgtaag
     3421 caaaagaaac aagataatag cgcgttgagc acgcacgaat cgaaccatga aaaaccccct
     3481 gtaaaatcga aaacccctaa attcggacag aggcgttatc tgctcctaga gtctgaaatt
     3541 gtcaatgggc gtcaagcgac gatgggcttg gatatttgac cttttggaaa agttccccca
     3601 gagtgttcgg tggggaaatg ggggagtgat cgattgaaat ttctacacct attgcgaacc
     3661 gctctggttt tgagcgcgtt cagtgtctat tcgctgcagc cgacttgggc ggccgaaata
     3721 aaacctgatt gcaaccaagt cgtgcaaacc tctggtgtca gaggcaaact ttcccgttgg
     3781 tggaaattat taaaagcccc tttcggaaaa tcgcgatccg aggatcgcag attttcgatt
     3841 gatgtgctga atgcagaaat ttcggatttg ccctcggagg tacaaaaaag aattctcgtc
     3901 gatttgttac ctcaacttcg gccctactcc ggtgaagacc ttgtagaggt attgcagaaa
     3961 gtgcgcgatg aaacgcgagg ttatttttat ttaaatctac gccgcggaat taccaaaaaa
     4021 atcgacagtg tcacccaaga gaaactacgt atgcttttaa gtcgtgaaga ccgtcgtgct
     4081 gcggtggaaa ttctcaacga ccgttggatg gtggacgact tagatttgcc gaaagttgag
     4141 ttaactgaag caaatctcga accccaaggt gatgggcgaa tcgttctttg gaaactgtgg
     4201 gaccaactag aagcagacaa acttggatta tacgatgctc gcgaactttt gctgctcctc
     4261 gaacaagtgc cagtcgggca tgcacgaggg tttaatgatc acgggttacc ccgcggtgtc
     4321 gagcgacgca ctggtcgact accttacgac caatttttag caatcatggg tgatttcgcg
     4381 caaaaaacgg gggttaggct agaactcaaa tccattccga tggatcttct agttaccgcg
     4441 gtaattttag accgcctggc ccatcttcca aatgctagcg acgttcaaaa acagcgagcg
     4501 ttcgaaatgg cggttctaat ttttcgcgaa tcgcgccatt gggagaaaat ctcccagccc
     4561 gagcaatgga attggttagt attctttcgg cgacttgggc atatcgatta aagagcttgt
     4621 ccgtctaaaa gtcgacgcgc agttttaaaa atttcgtcga acatcggttc agttagtctt
     4681 ttggtgaaag tattttgctg gctcgggtgg taggaaccta gcagccagcg cccgtggggc
     4741 aactgggtgc aaacaccgtg ggaaaatttt ggaatttttg tttttggatc tattcccaaa
     4801 ggccctgccc gtagagtttt gatcgcctct ttgtaggcaa tagctccaag cgccaaaatc
     4861 acgcgcaccc gggagagata ttcgaactca gccataagat tgggcgcgca gttttcaatt
     4921 tcttttaagg tcggtttatt ctctggagga gcgcagtgaa cggtggccga gatccacgca
     4981 tcacgaagct gcaaaccatc cgcggcgttt cgcgactcag gttgactggc gaatcccgcc
     5041 ttgtaaagag ctcggtaaag ccaaagcccg gagttgtcac cggtgaacat tcgacctgtg
     5101 cgattcgctc cgtgagccgc cggcgcaaga cctaacagaa acagccgagc ggcggggtct
     5161 ccgaaactcg gaactggttt tccccaatag gtttctgctg cgaaagctgc acgttt
//
LOCUS       DAOMXO010000073         5156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8590_length_5156_cov_4.592433, whole genome shotgun sequence.
ACCESSION   DAOMXO010000073 DAOMXO010000000
VERSION     DAOMXO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8590_length_5156_cov_4.592433"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1342
                     /gene="ftsA"
                     /locus_tag="PLH57_02535"
     CDS             95..1342
                     /gene="ftsA"
                     /locus_tag="PLH57_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRK01517.1"
                     /translation="MARTPKKDFIVGLDIGTTKICAIVGEVTESGVDIVGIGSHPSTG
                     LRKGVVINIESTVESIRKAIDEAELMAGCKITSVYAGIAGGHIKSFNSHGIVAVRNKE
                     ITQEDVNRVLDAARAVAIPMDREVLHVVPQEYIIDDQDGIKEPIGMNGVRLESKVHIV
                     TGAISSAQNIVKSCNKAGLNVAEIVLEPIASAEAVLTQDERELGVVLVDIGGGTSDIA
                     LFSQGSLVHTGVLAVGGNHITNDIAVGLRTPQNEAEKLKVRYGCAMSSLVKSEDTIEV
                     QSVGGRKPRTVPRKLLAEIIEPRVEEIFSLVQREVQKSGYQELMASGIVITGGSTILE
                     GMPELAEFIFDMPVKRALPMGVGGLKDVVNSPKYSTGVGLLKYAMRKQMGSKGKFPIR
                     MSSTINAYEKVRASMSGWIKDLF"
     gene            1394..2449
                     /gene="ftsZ"
                     /locus_tag="PLH57_02540"
                     /pseudo
     CDS             1394..2449
                     /gene="ftsZ"
                     /locus_tag="PLH57_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
     gene            2929..3570
                     /locus_tag="PLH57_02545"
     CDS             2929..3570
                     /locus_tag="PLH57_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01518.1"
                     /translation="MKSIVLVLVFSLFAFAKVERSAWSYEIQTVSPVLEMVDADLRRM
                     YPDLKRYEIREITSAALSNKKVQQKLKKISGVDFGKDLDSTNLSRTLYVAFDSDDRFI
                     GIAFGMAHRLPSGIIQLFLATKGSSVGPLKIDSLFFQRSASPNVAILKDPGYLSQFKQ
                     FSTHRVPERAWVRPPLHVERKRLDKEVLEDHHAFVRAVRKTLGWLQVIRGLNG"
     gene            complement(3560..>5156)
                     /locus_tag="PLH57_02550"
     CDS             complement(3560..>5156)
                     /locus_tag="PLH57_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRK01519.1"
                     /translation="KLVVFIAIDQLRADYLTRFREFLLPPVQKNGLPGGFRYFTDKGA
                     FYPTAETGHGTNVTAPGHATLSTGALPYTHGIVANRWFDSETNQVVYGLEDHNSKVLT
                     QTDAPKEKGRSPKNLLSTTISDEIKTAFGGKSIVGTVAIKDRAAILMGGHRADYALWF
                     DDKIRGLTTSSYYSKEMKLPQWVEQWNKTNWIDKNVPLAWERMLPRGAYAFSTQAPTK
                     NVYQNYKGLGQEFPHQIEKGGVAFQGTPWGSAYVLEAALEMASKTGMGKDEITDFLGV
                     GISSFDIAGHAFGANSEQMQDFFIRQDRLLASFLKKLQHTVPGGLASITFVLTADHGV
                     TEIPSIAQSVGMPFKAMAPEDFYAKAESFLEKEFGLNRKNKPVAYISENNLYLNTKEL
                     VVNKLDPEVVAQKLARWLATQPCFGMAYTKWELLAGKHANLHFSKRLATSTHAVRSGD
                     VVYSTTGICPQYEHYGVQGAEHGHSYTHDASVPIMIVGKSFKPGRYFQKATLADIAPT
                     IAASVGVSPTTGSEGRVLAEIIR"
BASE COUNT         1338 a         1206 c         1371 g         1241 t
ORIGIN      
        1 gaccgatccg gtggtcggcc tgctgatggg gcagacggcg gagaacctgg cctgcttttc
       61 aaaagcgaat ttcattgtct tagggaaggg acacatggcg cgtacaccca aaaaagattt
      121 cattgtcggt ctcgatattg gcaccaccaa gatctgtgcg atcgttggag aagttacgga
      181 gtccggcgta gacatcgttg gaatcggaag ccatccgtcc actggtctgc gcaaaggtgt
      241 ggtgattaat atcgaaagca ccgtagagag tattcgtaag gcgattgacg aggccgaact
      301 catggcgggc tgcaaaatta cctcggtata cgctggaatt gctggtgggc acattaagag
      361 ttttaatagc cacggcattg tcgcagtacg aaataaagag attacccaag aagacgtaaa
      421 tcgcgtttta gacgctgcaa gagcagttgc gataccgatg gatcgggaag tactgcacgt
      481 cgtgccacag gaatacataa ttgacgacca agacggaata aaagagccga taggaatgaa
      541 cggtgttcgt ctggaatcga aagtacatat cgtcactggt gcaatttcca gcgctcaaaa
      601 cattgtaaag tcctgcaata aagcaggtct caacgtggcc gaaattgtgc tcgaacccat
      661 cgcatcggct gaagccgtac ttacccaaga cgagcgggaa ttgggtgtgg ttttggtgga
      721 tatcggcggt ggcacctccg atattgccct attttcccag gggtcactgg ttcatactgg
      781 cgtattagct gtgggcggta atcatatcac caacgatatt gccgttggac tgcgaacgcc
      841 tcaaaacgaa gctgaaaaat taaaggtacg gtacggctgt gccatgtcgt cgctggtgaa
      901 atctgaagac acaatcgagg ttcagagcgt aggcggccgt aaaccgcgaa cagttccacg
      961 caagttgttg gctgaaataa tcgaacctcg ggtcgaagaa attttttcgc tagttcagcg
     1021 agaggttcaa aaaagcgggt accaggaact aatggccagt ggaattgtga tcactggtgg
     1081 cagcaccatt ctagagggta tgccggaact tgctgagttt atctttgaca tgccagtcaa
     1141 acgcgctctc ccaatgggag tcggggggtt aaaggacgtg gtgaatagtc ccaaatactc
     1201 taccggcgta gggctcttaa aatacgcaat gcgcaaacaa atgggcagta aaggaaagtt
     1261 tccgattcgg atgagcagta cgattaatgc atatgaaaaa gtgcgggcat ccatgtcggg
     1321 ttggatcaaa gacctgtttt aatcagaaca acgccgagcg gagtctcggt tcaattaatc
     1381 ggaggaacaa cggatgttcg acttcactga agagcaaggt actcaaggag caaaaattaa
     1441 ggtcatcggc gtcggcggcg gcggtggaaa cgcggttaat acgatgattc gcatggggct
     1501 ttcgggcgtg gaattcatcg cggccaacac cgatcgccaa gccctcgaag cttctcttgc
     1561 ttcggtgaaa gttcagcttg gcaacgagtt aacaaaagga ctgggcgctg gaagcaatcc
     1621 agaagttggt cgtcgcgctg cgatggaaga ctacgccaaa atttcggaac tcctagctgg
     1681 tgccgacatg gtgtttgtga ccgcgggaat gggaggtggc actggaaccg gcgccgctcc
     1741 gattatagca aaaattgcga aagaagtagg ggccctaaca gtaggggtgg ttactaaacc
     1801 ctttcagttt gaaggtcgca agcgcatgaa gcaagccatg gaaggattaa acaatcttcg
     1861 cgagagcgta gatagcttga tcactattcc aaacaatcgt ttgctaacga ttgccggcgc
     1921 taccttaaca atcattgata cttttaagaa ggctgatgaa gtattgttga atgcggtcca
     1981 aggtatatcg gatctgatca accacactgg gcttatcaac tctgacttcg cggacgtgaa
     2041 aacgattatg tcgaacaaag gtttggcgct catggggatc ggcgttgcga gcggagaaaa
     2101 tcgtgcaatc gacgcagcga cttcggcgat ttcatcaccg cttctcgaag atgtttcgat
     2161 acatggcgct accggcataa tcattaacat caccggcggt tccaatatga aaattcatga
     2221 agttaacgaa gcaacttcac ttattatgga agctgccgat gaagacgctg aaatcatttt
     2281 cggtaccgtt atcgatgaga gcatgaatga ttcggtgaag gtcactgtga ttgcaaccgg
     2341 tctaggggtt accgaaagta aggtggcagc cgcaatcact ccggagcaaa aagcccaagc
     2401 cgttcaggaa atgaaagcag cgcaagctga agtttcagag cagccacccg tctcagaacc
     2461 aacggtttcg gcccctgtag aggcgagcga agtggtagag cccaaccaag cgaatcagaa
     2521 cattgaatta aatctttcgg ctgaaatcgc taacgaggta gcagctcgaa atcaggccgc
     2581 tcccatgcag gcaacacctc cgccagctcc gcagcgcgct atgtcaaaca cggttgctcc
     2641 gagtcaggtg ggtggcgtga atgaaaaact catggcttac gcgaaaatgg ctgcgccatc
     2701 ttcgtactgc gatgatggac tcgacgaagc ggccgacatg gatgcaggcc cgctgagcga
     2761 tccaatgccg gcaactggaa ctcccatgcc cggcgcaatg acgccaaaac acgccgaaat
     2821 tgcgcgtgca aaagcaattg cgcaaaagct gggtctaacg aaccttaccg acgacgagta
     2881 tgacatacct acgtttattc gtcgtcagca agaggcaggc cctgacgttt gaaaagcatt
     2941 gttctagttt tagttttttc gctctttgca ttcgcgaagg tcgagcgatc ggcctggtcc
     3001 tacgagattc aaacggtgtc acccgttttg gaaatggtcg acgccgatct tcgccgaatg
     3061 taccccgatt taaaaagata cgaaattcga gaaatcacta gcgcagccct ttcaaacaaa
     3121 aaagttcagc aaaaacttaa aaaaatatcc ggcgttgatt tcggcaaaga cttagattcg
     3181 accaatcttt cccgcacgct ttatgtggca ttcgattccg acgatcggtt tataggaata
     3241 gcgtttggca tggcccatcg attgccttcc ggcattattc agctattcct ggccacgaaa
     3301 gggtcgagtg tcgggccact aaaaatcgat tctcttttct ttcagcgatc tgcttcccct
     3361 aatgtcgcta tccttaaaga ccccggctac ttgtcgcagt tcaagcagtt ttctactcat
     3421 cgagttcccg agcgcgcgtg ggtgcgtccg ccacttcacg tagagcgcaa acgtctagac
     3481 aaagaagttt tggaagatca tcacgccttt gtccgagcgg tccgaaaaac tctagggtgg
     3541 ctgcaagtca ttcgtgggct caacggataa tttcagctaa taccctgcct tcggatccag
     3601 tcgttggtga gactcctacc gaggcagcga tggtcggggc gatgtccgcg agcgtagcct
     3661 tttgaaaata gcggccaggt ttaaacgact tgccgacaat catgattggt accgaagcgt
     3721 cgtgagtgta actatgtccg tgttcagcgc cttgaacccc gtaatgttcg tattgtgggc
     3781 aaattcctgt tgtcgaataa accacgtcac ccgaacgcac cgcgtgtgtg ctagttgcca
     3841 agcgtttcga aaaatgtaaa ttggcgtgtt tacctgccaa taattcccac ttggtgtagg
     3901 ccatgccaaa acagggctgc gtcgcgagcc agcgagccaa cttttgtgct acgacttcgg
     3961 ggtctaattt attgaccacg agttccttag tgttgagata gaggttgttt tcgctaatgt
     4021 atgcgactgg tttatttttc cgattaagac cgaattcttt ttctaaaaag ctctcagcct
     4081 tggcataaaa atcttccggc gccattgctt taaacggcat gccgacagat tgagcgatag
     4141 aaggaatttc ggtaacaccg tgatcggcag tcagcacgaa agtaatcgag gcgaggccgc
     4201 ccggcacagt gtgctgaagc ttttttaaga aactcgccag taggcgatcc tgtctaataa
     4261 agaaatcctg catttgttcg gagtttgctc cgaatgcgtg gccagcaata tcgaaagatg
     4321 aaattccaac ccctaagaaa tcggtaattt catctttacc cattccggtt ttcgaggcca
     4381 tttcaagcgc ggcttcgagc acataggccg aaccccaggg agttccctga aaggctacgc
     4441 cgcctttttc aatttggtgt ggaaactcct gaccgagtcc tttgtaattt tgataaacgt
     4501 ttttggtcgg agcttgcgtt gaaaaggcgt aggctcctcg tggcagcatg cgctcccagg
     4561 ctagaggtac gtttttatcg atccaattag ttttgttcca ttgctccacc cactgcggca
     4621 gtttcatttc tttggagtag taggaactgg tagttaagcc tcgaatcttg tcgtcaaacc
     4681 aaagtgcgta atccgcgcgg tgcccgccca tgagaatggc ggcgcggtcc ttgatcgcca
     4741 cggtgccgac aattgatttt ccaccgaagg cggtcttaat ttcgtcagat atggtggttg
     4801 agagtaaatt tttgggtgat cggccctttt ccttgggggc gtcggtttgc gtgagcacct
     4861 tagaattgtg atcttccaac ccataaacca cttgattggt ttcggaatca aaccaccgat
     4921 tagcgacgat tccgtgggtg taaggaagcg ctccggtaga aagagtggca tgacccggcg
     4981 cggtaacgtt ggtgccgtgc ccagtttcag cagtcgggta aaaggcgcct ttgtcagtga
     5041 agtagcgaaa tccacccggg agcccatttt tttgtactgg tggaagtaag aattcgcgaa
     5101 aacgggttag gtaatctgcg cgcaactgat caattgcgat gaaaacgact agtttc
//
LOCUS       DAOMXO010000074         5123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8672_length_5123_cov_4.598658, whole genome shotgun sequence.
ACCESSION   DAOMXO010000074 DAOMXO010000000
VERSION     DAOMXO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8672_length_5123_cov_4.598658"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLH57_02555"
     CDS             complement(<1..620)
                     /locus_tag="PLH57_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRK01520.1"
                     /translation="MSGTRRVRTHTCGELTSKHIGSTVTLVGWAHKRRDHGGVIFIDL
                     RDRYGVTQTVVDPTNAAALKEAEKVRNEYVVFMRGSVRARPSGMTNSKLPTGEIELYV
                     EQCEILSESKPLPFPIEDGSEVSETLRLKYRYLDLRRPSLQNNLITRHRVGQLVRNYL
                     DENGFLEIETPILYKSTPEGARDFVVPSRVNPGTFYALPQSPQTLK"
     gene            complement(542..1777)
                     /locus_tag="PLH57_02560"
     CDS             complement(542..1777)
                     /locus_tag="PLH57_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01521.1"
                     /translation="MASKKNQPRLDTDRWPSHWDFCIIGNGVAALWMAHHAWRTKRSV
                     LWIGSDDAYTSARSLCSHAWHWGLSESTANDFRELMSLEKETPNFELAYYDARSSKRF
                     RSLSEVKLEWGEHEVAYFESLLPTEPAMDLWSWNNQIQGFHSTSELPSGPSTVELFSD
                     PVFVRLHRWPVQAMETKDGRLMRVAIGGGDLEPVWVSASRFVLTDYDENFASLVQDSN
                     DAEVLAGASKGRTIRPGFGLRLRHKNYSNPLSQTVIIPLTVSPEKGKASHVVGRFVEG
                     AQGLESVWFGFLTDEEVEDNNEILKKIKHAKRAIDRALPGFLDSIEREAVTFEPRMFA
                     KEFARDSGRSKEILSGKAKQGSSENSAAKPVLGAWLLSDQFGLEPLVKGFLKCLAPDV
                     SERTHVENSLPNTLEVPSP"
     gene            complement(1866..3479)
                     /locus_tag="PLH57_02565"
     CDS             complement(1866..3479)
                     /locus_tag="PLH57_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRK01522.1"
                     /translation="MRLTPLIYSKFILSVLGALSLGVLSGCASGPRTNNDAIGHVIRE
                     SDRPSSAERDARAASVPGQPLEVWGQKIKNTTFDYPVIVNPRVEHWISYFTGKGRRYF
                     TKYLERGRYFIPEIAKVLRASNMPQDLVYLAMIESGFNNSARSHAKAVGPWQFIRGTG
                     RRYGLAIDYWLDERRDTRKSTMAAIKYLRELYEEFGSWELAAAGYNAGEGKVRRAIAK
                     YKTSDFWQIARHRFFRPETRDYVPKIMAAAIISKNPEQFGFEDPFARDGKSRNPTILL
                     DADVAEADEVTPKELEEPIAAAEETADDPAEPPAVNDVEVDTEVASEDGDESDDVEHA
                     AVSSGPNPVMTPSVYMVANPNEQILEFEVKGPADLFAISKASGLPYSSLKLLNPEVLR
                     WLTPPYAKTYRIKLPASVKSRFLATYNDASFDRRVAYTQYKVQRGDSLKSIARRFKTE
                     TDAIREVNSPGTGLAVGKTINLPIPTGYKRVIASMYEEKPIPSRKRRRRAKRVRKADS
                     SRAEIRHHSRVQPKLRRIAETSVQRTSVQ"
     gene            complement(3580..4863)
                     /locus_tag="PLH57_02570"
     CDS             complement(3580..4863)
                     /locus_tag="PLH57_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01523.1"
                     /translation="MGDRSSSENHQSKNKLGTLGPFAPFALVVFALLPGLFHAGAAVF
                     QYLGPSASAAVLSAPTGRDSGEHRYQDRYNIEDHRLLLVLGLDLLVRAERVVHKEFGT
                     FTNALGRVPDLLGSSTLNYRVEVAKASPSQLLIRATGQGFRTMFDTKSVAGDRLFIDQ
                     NFEIKSNFPLPDPPKEYLRTLAKTVLFRLAREKAVTEPKPDILKIWEGVYGGYFRYER
                     RPRAQSEGEFAWVAVGLAGSTGGETITLDESDQLIPPNLFQWVQRYRATHWIEQEVRA
                     KLELVYLGERMSHDHTGGYVFESGDLSAYWNSLSDLASSDASYFLRGISKDSELGYRA
                     EIAEKASGRFWTINGYGQLSENAETAVIIQQFDAARRKIASHGGLQADLGTQQSSVSG
                     GNAGTPFIVSDPESGKNLMIEPVSPTEVNRGREKF"
BASE COUNT         1236 a         1377 c         1210 g         1300 t
ORIGIN      
        1 tgcttcaaag tttggggtga ctgaggaagc gcgtaaaacg tgcccggatt tacccgggat
       61 ggaaccacga agtctcgtgc tccttcagga gtgcttttat agagaattgg cgtctcgatt
      121 tcgagaaacc cattttcgtc taagtaattg cgcaccaact ggccaacgcg atgacgagtg
      181 atcagattgt tctgcaatga gggtctgcgc aaatcaagat aacgatactt caaacgcaaa
      241 gtttcagaca cttcgcttcc gtcttcgatc ggaaaaggta gtggcttact ttcacttaaa
      301 atttcacact gctcaacgta aagttcaatt tctccagttg gtagtttgga attggtcatg
      361 ccggatggac gcgctcgcac cgagccacgc atgaacacga cgtattcgtt acgaactttt
      421 tcagcctctt tgagggccgc agcgttagta ggatctacga cggtctgggt aacaccataa
      481 cgatcgcgaa gatcaataaa tatcactccg ccgtggtctc gtcttttatg agcccatcca
      541 actaaggtga cggtacttcc aatgtgtttg gaagtgagtt ctccacatgt gtgcgttcgg
      601 acacgtcggg tgccagacat tttaaaaacc ctttcactaa cggctccagg ccgaattgat
      661 ctgacagcag ccaagcacct agaacgggct ttgcggccga gttctctgaa ctaccctgct
      721 ttgcttttcc actcaatatc tctttcgatc gacctgaatc tcgggcgaat tccttggcga
      781 acattcgcgg ctcaaaggtc acggcttctc gttcaatgct gtccaaaaat ccgggcaatg
      841 cgcggtcaat cgcccgcttt gcatgtttga ttttctttaa aatctcgtta ttgtcttcga
      901 cttcttcgtc ggtcaaaaaa ccgaaccaga cactttccag cccttgcgcg ccctctacga
      961 atcgaccaac cacatgggag gctttgcctt tttcggggct caccgtgagc ggaataatga
     1021 cggtttggga aagcggattt gaataatttt tgtggcgcaa tcgcagacca aatcctggac
     1081 gaattgtgcg ccctttactc gcgccggcca atacctcggc atcattcgag tcttgcacca
     1141 gcgaagcaaa gttttcgtcg taatcggtca gcacaaagcg gctcgccgac acccaaactg
     1201 gttcgagatc tcccccacca atagcgaccc gcattaggcg cccgtctttg gtctccatcg
     1261 cttgcactgg ccagcgatgc agccgaacga aaaccgggtc cgaaaatagc tccacggtcg
     1321 aggggccaga aggcagttcg ctcgtactgt gaaaaccctg gatctgatta ttccaactcc
     1381 aaaggtccat agccggctca gtaggtaaca acgactcaaa ataagcgacc tcgtgctcgc
     1441 cccactcgag ctttacctct gagagcgaac gaaaacgttt cgaagaccgc gcgtcgtaat
     1501 aggctaattc gaaattcgga gtttctttct cgagcgacat taattctcga aagtcgttgg
     1561 ccgtcgattc cgagagtccc cagtgccagg catgagaaca gagcgagcgc gcagaagtgt
     1621 aagcatcgtc gctgccaatc cacagaacgc tacgctttgt acgccaagca tggtgcgcca
     1681 tccaaagggc ggcgacaccg ttgccgatga tgcagaaatc ccaatgggag ggccaacgat
     1741 cggtatctag acgaggctga ttttttttgg atgccatgaa gattccctaa tagtctctga
     1801 cgcccatgac tttaagcgat ccaatgtgga ttggtcaact gaacgaaata ggatctatga
     1861 ggatattatt gaaccgacgt tcgctgaacc gacgtctcag cgattcgccg cagcttcggc
     1921 tgtacccgtg aatggtgccg tatttcggcc cgactgctat ccgccttacg cactcgcttg
     1981 gcccttcggc gacgtttccg cgaaggaatg ggtttttctt cgtacatact ggcaataact
     2041 cgtttatagc cggtcggaat aggtaaatta atggtcttgc cgaccgccaa tccagtgccc
     2101 ggactgttca cttcgcgaat ggcatcggtt tcggttttaa atcgccgcgc gatcgatttc
     2161 agcgaatcgc cccgttgaac cttatactgg gtgtacgcca ctcttcgatc aaagctcgca
     2221 tcattgtagg tagccaaaaa tcttgatttt actgaagctg gcaatttgat ccggtaggtt
     2281 ttcgcgtaag gcggcgtgag ccagcgtagc acttcggggt tgagcaattt gaggctagaa
     2341 taaggcaatc cagaggcctt tgaaattgca aatagatcgg ctgggccttt aacttcgaat
     2401 tccaaaattt gttcattcgg attagcgacc atataaaccg aaggggtcat caccggattc
     2461 ggtccgctgg agaccgccgc gtgttctacg tcgtcgctct cgtcgccgtc ttcgctagct
     2521 acttcagtgt cgacttctac atcgttcaca gcaggcggtt cggccggatc atccgccgtt
     2581 tcttccgccg cagcgatagg ttcctcaagt tccttgggag ttacctcgtc tgcttccgct
     2641 acatcggcat ctaacagaat tgtcggattt cgcgatttcc catctcgcgc aaaaggatct
     2701 tcgaacccaa actgttccgg atttttcgaa attattgccg cagccatgat tttcggcaca
     2761 tagtcccggg tttcaggcct gaaaaaacga tggcgtgcga tttgccagaa atccgaggtt
     2821 ttgtatttgg cgatcgcccg ccgcactttg ccttccccag cattgtaccc agcagccgcc
     2881 aattcccagc tgccaaattc ctcatagagt tctcttaaat atttgatagc cgccatggtg
     2941 cttttacgag tgtcacgacg ctcgtcgagc caataatcaa tagccaagcc gtaacggcga
     3001 cccgtaccgc gtataaattg ccaaggacca accgcttttg catgcgaacg cgcggagttg
     3061 ttaaacccac tttcaatcat cgcaagatac actaagtctt gcggcatatt ggacgctctt
     3121 agtactttgg caatttcggg aataaaataa cgacctcgtt cgaggtactt ggtaaagtac
     3181 cgacgccctt tgcctgtaaa ataggaaatc cagtgttcaa ccctgggatt cacaataacc
     3241 ggatagtcga aggtggtgtt cttaattttt tgcccccaca cctcgagcgg ttggccaggc
     3301 actgaagcag cacgagcgtc gcgctccgca gacgaaggac gatctgattc gcgaataaca
     3361 tgacctattg cgtcgttatt agttcgaggc ccagaggcac acccgctcag aacccctaac
     3421 gaaagggctc ccaggacact caaaataaac ttgctgtaga tcaaaggtgt taatcgcatt
     3481 gtgttggttt tatccttcca tccatggaaa aagagcccga ctctgtttta cctcgctcgg
     3541 ccaaggtcta aacagtttaa ggaaacttag tcgaacctgt caaaattttt cacgtccgcg
     3601 gttcacttcg gtaggcgaaa ctggttcaat cattagattt tttccagatt cggggtctga
     3661 cacgataaac ggagtgccgg cattgcctcc ggatacggag ctctgctgcg tgcccagatc
     3721 ggcctgcaga ccaccgtgac tcgctatttt tcgtcgggcc gcgtcgaatt gctggataat
     3781 aactgcggtc tccgcatttt ccgaaagttg cccgtagccg ttgatagtcc aaaatcgacc
     3841 gctggccttt tcggcaattt ctgcccgata acctaattcc gagtcttttg aaattcctct
     3901 taaaaaatag gatgcgtcac ttgatgccag gtctgacaaa gaattccagt acgccgataa
     3961 atcgcctgat tcaaaaacgt aaccaccagt gtggtcgtga ctcattcgtt cgccgagata
     4021 cacgagctct agttttgccc gaacttcctg ctctatccaa tgtgtggcac gatagcgctg
     4081 cacccattgg aacagattcg gcggtataag ctggtcgctt tcgtcgagcg taatagtttc
     4141 tccgccagtt gagccagcca atccaactgc cacccacgca aattcaccct cactttgcgc
     4201 acgagggcgc cgctcgtaac gaaagtaacc tccatacaca ccttcccaaa ttttcaagat
     4261 gtctggtttg ggctcggtca ctgccttttc ccgcgcaagt ctaaacagca ctgtcttggc
     4321 caaagtgcga agatactctt ttggggggtc cggcaacgga aagttactct ttatctcaaa
     4381 gttttgatca ataaaaaggc gatcaccggc aacagactta gtgtcgaaca ttgtacgaaa
     4441 cccctggccc gtcgcccgaa tcaacaactg gcttggagat gctttggcca cttcgacgcg
     4501 ataattgagc gtactcgatc ccagaaggtc gggcacccga cctaacgcat ttgtgaaggt
     4561 tccgaactct ttgtgcacaa cacgctcggc tcgcacaagc aagtccaacc ccagaaccaa
     4621 caaaagtcgg tggtcttcaa tattgtagcg gtcttgataa cggtgctcgc ccgagtctcg
     4681 gcccgttgga gcgctcagta ccgccgccga agcactcggc cccaaatact ggaacaccgc
     4741 tgcgcccgcg tggaacagcc cgggcagaag cgcaaaaact acaagcgcaa aaggggcaaa
     4801 tggccctaat gtaccgagct tattcttgga ttggtgattt tcggatgagg aacggtcccc
     4861 catgcaacac tactcttccc ttgctgactc agaacccgca ccaaaaagtg atgcaaatac
     4921 tagacctttt tagttaacaa tgttcacttt tctttctctt tgattttaaa gacctttttt
     4981 aagattcggc gatttccccg gggatattat ttcgaattta ccgcctaagt tttggccagt
     5041 gacaagttct tagacaggag caagaagttt tcgctgaatt tgcactctgt ggccaaaatc
     5101 gatatatccc cgaagcgttt tta
//
LOCUS       DAOMXO010000075         5121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8678_length_5121_cov_4.326096, whole genome shotgun sequence.
ACCESSION   DAOMXO010000075 DAOMXO010000000
VERSION     DAOMXO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8678_length_5121_cov_4.326096"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLH57_02575"
     CDS             complement(<1..357)
                     /locus_tag="PLH57_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01524.1"
                     /translation="MKKNSVVSRGSSILLIWVLLFQLSLGTALTQAAGNDGTPTSDGV
                     PIIVDGSEVDSQNELSAAAASELSAIAQQMAEVADFHSRIDGAAPGSAAPTPIDNWLL
                     NDQGMRVFETVEIEKGH"
     gene            complement(538..2652)
                     /locus_tag="PLH57_02580"
     CDS             complement(538..2652)
                     /locus_tag="PLH57_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01525.1"
                     /translation="MKQPKFFYYAFVCAILSIMTNLDHAFGTDQNAESVKSTLQRIGK
                     FEVKQLVSPGFELLPLERKLFAMEIAKVIEAGHDIAWIQANESSLAIREVMHGLWKHR
                     EKFESLDRAALAEYLFLLHANHGNYDHRKNKKFVPEGLTLDRALNLSRVADAQNSANG
                     VSSKLEEQMLALEREIFAADYRPAFHADGKGQDIVADSAANFYGPGITKAEIQGLPPE
                     IGGHFLSYPVRGKSGNIELLEHRIGGRFDEYLRLVEHHLKNALQWALPEERPVIEAYR
                     VALRSGDPQDLAKADGLWALYKPRDIDFQFNFIEVYSDPLNSRGEWEGFMILLNQEPA
                     EVARVEKMRRHAAAFEAEMPVDEEFKKQGDFTPPKAEGAYLLYSGGANGERPFAGVNL
                     PNTTINQKIHEQFGSKSYTADNVMADLGEAPKEFSPEQMNVFYAPEYHELLKTVNTKL
                     ERTLQVAAHEIWGHGSGRSREGVRDTDLRDYYSALEEGRAEIASLYHMLGTLIRDTDI
                     LPQSWSDQQLRDFQTATILIFFTAHLRTYERLAPDAEAIRQAHQWGRQVMMNRLIAEG
                     ALEVDSDPALPAPWIRKIDLEKARQALGGLWKQIQRAKSTGDFELAAKLFDTEGGYQE
                     PHRKLRERIAKAIKDLNLPTEKAYLNPGFEAVRDSSGKVTDVKLVYEAPGDHAMERFV
                     DRQVRRATTLREALNCNNLVVE"
     gene            2770..3387
                     /locus_tag="PLH57_02585"
     CDS             2770..3387
                     /locus_tag="PLH57_02585"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK01526.1"
                     /translation="MRHYFILIAFLLAPLPAALAAPEAADSGLSAAQTTVQTIADYAR
                     NLDDKALKEKAKRRIEELVDFSELSVNALGEHASSMTAQQRAKVQNLLKTILTGTIYP
                     QAGKFFSDVNIAYSGESQAKADGRELVKVTSTVSKNNKRSKVEYWLKQTPTGMRVVDL
                     AIEGERWVENVQMQFDELIRQKGVNGLVARMQKRANELRQNQSKN"
     gene            3400..4761
                     /locus_tag="PLH57_02590"
     CDS             3400..4761
                     /locus_tag="PLH57_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRK01527.1"
                     /translation="MKKFVVFFCLPLTLAAAADLSQPQVSTVDVSRTVTLNEALKLGL
                     QNNLSILAERTVLEIAGARQSQARAANYPKVLGTAILAPINSVEGNAINAYENGKWGP
                     WLTSTLQILQPVYTWGKISSYKEAAAQGLEVAKAQTRKSESELVYEIKELYYGAVLAE
                     QLVDFLQGGKNDVDELVKKATEDQAKKRPDISRRDFYRLKIFAAEASYRLEEAKKLRE
                     LARHALSLKLGFHPDTETLPSESALSPIELPIPEESLLRARLATERPELRQLHHGIAA
                     KRALLEAEKANKFPMLFLGGQLELAYSNVRQKQNSPFAFDPYNRSQIGVGIGVQWNFD
                     FATTLANEQMGRAEISELERKEEYAQVGFVMELKKSLADLREMKTRVEESREAFQVGK
                     RWLVSETMGYSAGLGEVKNVIDAYLARAKTAKDHWEAIYRLNMAWAGLDKTVGSNLTP
                     NAL"
     gene            4821..>5121
                     /locus_tag="PLH57_02595"
     CDS             4821..>5121
                     /locus_tag="PLH57_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HRK01528.1"
                     /translation="MAPTSKSNAKVKPAEGKLGILMPGLGAVATTMIAGVEAIKQDIA
                     KPIGSLAWMGKMRLGKRNENRMENIRDVVSFSRPDQVVFGGWDIFKDNAYESALNA"
BASE COUNT         1333 a         1339 c         1242 g         1207 t
ORIGIN      
        1 atggcctttt tcaatttcga cggtttcgaa tactcgcata ccctggtcat tgagaagcca
       61 attatctatc ggagtaggag ccgcggaacc gggagccgca ccatcaattc gactgtgaaa
      121 atctgccact tcggccattt gctgcgcgat cgcactcagt tcgctggcgg cggcggcact
      181 cagttcgttc tgagagtcaa cttccgagcc atcgacaatt attggcacac catcggacgt
      241 cggcgtccca tcgttgccag cagcttgggt taacgcagtt cctagcgaaa gttgaaacag
      301 caaaacccag atcaaaagaa tcgaagaccc tctcgaaact acagaatttt tcttcattat
      361 tcgaaatacc cctgtctata ccctgcggac ataatttgtt ctttcgaaca gcattaggaa
      421 aaacggagtt accagaggac gacaaaatgc gtcaagcggg ggtcctcttt ttttgttgta
      481 tttgatcgaa aagcgcgaga ggggaggaag tttggccctt ccacccctat ctttaggcta
      541 ttccaccact agattattgc agtttagcgc ttcccgaagg gtggtggcgc gccgaacctg
      601 ccgatcaaca aatcgttcca tcgcgtgatc ccctggggcc tcgtacacca acttcacgtc
      661 ggtcactttg cccgaagagt ctctgacggc ctcgaaacca gggttgaggt aggctttttc
      721 cgtaggcagg tttaagtctt tgatggcctt ggcgattcgc tctcggagct tgcggtgggg
      781 ttcttgatac cccccttcgg tgtcgaagag cttggcggct agctcaaaat cgccagtcga
      841 cttggctcgc tgaatctgtt tccacaaccc accgagcgcc tgacgagcct tttcgaggtc
      901 aatttttcga atccagggcg ccgggagcgc cgggtccgaa tcaacctcca gtgccccttc
      961 tgcgatcaaa cggttcatca tcacttggcg gccccactga tgtgcttggc gaatggcctc
     1021 agcgtcggga gccaaacgtt cgtaggttcg caaatgcgcc gtaaaaaaga ttaagatcgt
     1081 cgctgtttga aaatcacgca actgttgatc ggaccagctt tggggcaaaa tgtccgtgtc
     1141 gcgaatcaag gtgccaagca tgtgatagag agaagctatt tccgctcggc cctcttccag
     1201 agccgaatag taatctcgca aatcggtgtc gcgcacgcct tcacgggaac gccctgaacc
     1261 atgcccccaa atttcgtgcg cagctacttg aagcgtgcgc tcaagcttag tgttcacggt
     1321 ttttaggagt tcgtggtatt cgggcgcgta aaacacgttc atctgttcag ggctaaactc
     1381 ctttggagcc tcacccagat ccgccatcac gttgtcggca gtgtaggatt ttgatccgaa
     1441 ctgttcatga atcttttgat tgatggttgt gttcggaagg ttcacaccag caaacggacg
     1501 ttcaccgttg gcccccccgg agtagagaag ataggcgcct tccgctttgg gtggcgtaaa
     1561 gtcaccctgc tttttgaatt cctcgtcgac tggcatttcg gcctcgaaag cagcagcgtg
     1621 tctgcgcatt ttctctacgc gagcaacttc ggcgggctct tgattgagaa gaatcataaa
     1681 accctcccac tcgccccgcg agttgagcgg atcgctatag acctcgataa aattaaactg
     1741 aaagtcgata tcgcgaggtt tataaagcgc ccacaaacca tcggccttag ctaagtcttg
     1801 cgggtcgccg cttcgaaggg caacacgata ggcttcaatt acggggcgct cttcaggcag
     1861 agcccactgc aaagcgtttt ttaaatggtg ttccaccagg cgcaaatatt cgtcgaatct
     1921 tccgccaatc cggtgttcaa gcagttcaat atttccagat ttaccacgca ccgggtagct
     1981 tagaaaatgg ccgccaatct ctggtggcaa cccttggatc tcggccttgg taataccggg
     2041 gccataaaaa tttgcagccg aatcagcaac aatatcttgc cctttgccgt cggcgtgaaa
     2101 cgccgggcga taatccgccg cgaagatttc gcgttcaagc gctaacattt gttcttcgag
     2161 ttttgagctc acaccgttgg ccgaattttg ggcgtccgcc acacgtgaaa gattaagcgc
     2221 acggtctaaa gttaaccctt ctggaacaaa tttcttattt tttcgatgat cgtagttacc
     2281 gtgattggcg tgtaataaaa acaaatactc cgccagggca gctcgatcca aactctcgaa
     2341 cttctcgcga tgtttccaca atccatgcat cacctcacgg atcgcgagcg acgattcgtt
     2401 tgcctgaatc cacgcaatgt cgtggccggc ctcgataact ttggcaattt ccattgcgaa
     2461 cagcttgcgc tcaagcggca ggagttcaaa gccgggggac accagctgtt tcacttcgaa
     2521 cttcccaatc cgttgcaacg tacttttaac agattctgcg ttttgatcgg ttccgaaagc
     2581 atgatcgaga tttgtcataa tcgaaaggat agcgcacacg aatgcataat agaaaaattt
     2641 tggttgtttc atgatttccc cctaagaact taaccaaact gaactaacca actggtaggg
     2701 attggtcaat gcttgacgtt cgtgtgctca cttgcactcc gactcgaaaa ggcgtatttc
     2761 taggggccta tgcgacatta ttttatattg atagcctttc ttttagctcc gcttcctgcc
     2821 gcccttgctg ccccagaagc agcggattcg ggcttaagcg ccgcacaaac cacggttcaa
     2881 accatcgcgg attatgcccg caatctcgac gataaagctc taaaagaaaa agctaaacga
     2941 aggatcgaag agttagtcga tttttcggaa ctatccgtca atgctttggg tgaacacgca
     3001 agttcaatga ccgctcagca acgtgccaaa gttcaaaacc tactcaaaac cattctcacg
     3061 ggcaccattt acccccaggc cgggaagttt ttttcggacg tcaacatagc ctactctggg
     3121 gagagccaag caaaagccga cggaagagaa ctcgtgaagg tgacctcgac tgtttcaaaa
     3181 aacaacaagc gatcgaaggt tgagtactgg ctcaaacaaa ctcctaccgg aatgcgcgtg
     3241 gtagaccttg cgatcgaagg cgaacgctgg gttgaaaatg tacaaatgca attcgacgaa
     3301 ctcattcgcc aaaaaggcgt aaacggtttg gtcgctagaa tgcagaagcg ggcaaacgaa
     3361 cttcgccaaa atcaatctaa aaactgattt ctatttgttg tgaagaagtt tgtagtattt
     3421 ttctgtttgc cgcttactct agccgccgcg gcggacttga gccagcctca agtctcgact
     3481 gtcgatgtca gccgcactgt cacgctgaat gaggcgttaa agctaggact acaaaacaat
     3541 ctttccatac tcgccgaacg tacagtgctc gagatcgccg gagctcggca gtctcaagcc
     3601 cgagcggcca actaccctaa agtgctcggc actgcgattt tggcacccat caattccgtc
     3661 gaaggcaacg ccatcaacgc ctacgaaaat ggcaaatggg gcccctggct caccagcacg
     3721 ttacaaatac ttcagccggt ctacacctgg ggcaaaatta gttcttacaa agaagctgcg
     3781 gcccaaggcc tcgaagttgc caaagcccaa actcgaaaaa gcgaatcgga actggtctat
     3841 gaaatcaaag aactttatta cggcgcagta ctggccgaac agttggtcga ctttctccaa
     3901 gggggaaaaa atgatgtcga cgaactcgta aaaaaagcaa ccgaagacca ggccaagaaa
     3961 cgacccgata tttcgcgccg agacttttat cgactgaaaa tttttgctgc cgaagcgtct
     4021 tatcgactcg aggaggccaa aaaactacgg gagcttgcac gccacgcact ttctttaaaa
     4081 cttggttttc accctgatac cgaaacatta ccgagcgaat cggcgctctc accaattgaa
     4141 ttgccaatcc cagaggagtc tttactccga gctcgcttgg caacagaacg ccccgaactt
     4201 cggcagctcc accacggcat tgccgcaaaa cgcgcactgc tcgaggctga aaaagccaat
     4261 aaattcccaa tgcttttctt gggtggccaa ctagaactgg cttattcaaa cgttcgacag
     4321 aagcaaaact ctccgtttgc gtttgaccct tacaatcgct cgcaaatcgg agtcggtatc
     4381 ggagttcaat ggaatttcga tttcgccacc acgcttgcca acgaacaaat ggggcgtgca
     4441 gaaatttcag aacttgaacg caaggaagag tacgcacaag ttggttttgt aatggaactt
     4501 aaaaaaagtc tcgccgatct acgagagatg aaaacccggg tcgaggaaag tagagaagcg
     4561 tttcaggtgg gtaaacggtg gctcgtttca gaaacaatgg gttatagtgc ggggctcggc
     4621 gaggtaaaaa acgtgatcga cgcttatctc gctcgggcca aaactgccaa ggaccactgg
     4681 gaagcgatct accgcctaaa catggcctgg gcgggcctcg acaaaaccgt aggatctaat
     4741 ttgacgccca acgccttata gacaaatcca gtcggacttg gtatctgggc cctttatatt
     4801 ctttgaaaga ggcactaacc atggcaccca cgtccaaatc caacgcaaaa gtgaaaccag
     4861 cagaaggaaa gcttggcatc ctaatgcccg ggctcggagc ggtcgcaacc acgatgatcg
     4921 ccggtgtaga agcaatcaaa caagatattg cgaagccaat tggctcactt gcttggatgg
     4981 ggaaaatgcg gcttggcaaa cgcaatgaaa accgaatgga aaacattcgc gacgttgtat
     5041 cattttctcg accagaccag gtggtttttg gtggatggga cattttcaag gacaacgcct
     5101 acgaatcggc acttaatgca a
//
LOCUS       DAOMXO010000076         5098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8731_length_5098_cov_4.574658, whole genome shotgun sequence.
ACCESSION   DAOMXO010000076 DAOMXO010000000
VERSION     DAOMXO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8731_length_5098_cov_4.574658"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLH57_02600"
     CDS             complement(<1..759)
                     /locus_tag="PLH57_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRK01529.1"
                     /translation="MKKLGLLGVVALMSASGAAHAVDTSMIKFEGNARLKYQSDNRVD
                     YQNNRRYTLLRVRPGMTVTPNSKTSLVIVPQFAKALGEPTHISSSTTANTEVSTSGSS
                     SLGAHGEFGVYEAYVGWQPLGWSEFYFGRQSLKYGDGLVIGQGNWGNHGRSFDALRAR
                     AKYGLGWSDLLFSKIKEYNTSDSSTIERGDKDLWALYSSWDLGQYFKNVDPYVFYQRD
                     RSSFNSAVKSDRYIADHLWTVGARLRSDVEKFDYR"
     gene            complement(786..2333)
                     /locus_tag="PLH57_02605"
     CDS             complement(786..2333)
                     /locus_tag="PLH57_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRK01530.1"
                     /translation="MKVTLVQPPALVAVDNYSTITLPPLGIAYIAGYLRSHHHEVTVV
                     DAVGAAVRKLEPWPKRKKRLIQGLSFEEILERIPSDTQVIGVTCMFTHAWPMVRDLLA
                     ALKERFPKAWLVCGGEHITSMHPTVLEQTPVDFCVLGEGEYTFRELIDLLGAGNLEQA
                     RTIQGVAYKSQAGQVVKNERRPRIKEVDNIPWPAWDLMDPMAYNEAEIYQGPQSNGKT
                     IPMLATRGCPYRCTFCSSPNMWTQLWKPRDPIDVVNEIESYMKKYGAADFQFQDLTAI
                     VKRDWILTFCKEILRRELKITWTLPAGTRSEVVDDECASYLMRSGCQHITFAPESGSP
                     RILDRIQKRVDLNSLEDSAKACLRNGMKVCLFIIVGFPFEEKSDIRATFKWLRHMARI
                     GVHEVATATFVPLPGTQLFGETSAISKIEVDDEYCHWMTAATSMLTVRSWNPRFSDRQ
                     ILFYKLYAMAQFYAVSYFFYPSRAFRLIRNFILHKQETKVDRVLREMVNKTAIVLKTK
                     LLSKKAS"
     gene            complement(2330..3502)
                     /locus_tag="PLH57_02610"
     CDS             complement(2330..3502)
                     /locus_tag="PLH57_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK01531.1"
                     /translation="MSSSSPIELSIVMPCLNEAETLEACIKKAKNFLERHQIAGEIVV
                     GDNGSTDGSQQIAERCGARVVSVPVRGYGAALYHATQAARGSYVIMGDSDDSYDFSGL
                     MPFVEKLRENYDLVMGNRFSGGIKKGAMPWKNRYIGNPALSGIGRLLYKIPVRDFHCG
                     LRGYSKSAFQKMDLHTTGMEFASEMVIKASLKGMRIVEVPTTLQPDGRSRAPHLRPWR
                     DGWRHLRFMLLFSPRWLFYYPGLALILSGLLLGSLLVTGPLDIGGIRLDIHALFYSAI
                     AVIIGFQSVSFYALAQISAVHKGLLPASKRMQKFLDHFVIEKGLVLGLLLFLAGVVGL
                     LVGVEQWGHSEFANIEPRQMMRIVIPSGLAATLGFNMILASFFMGVLQLPTRREPK"
     gene            3593..5041
                     /locus_tag="PLH57_02615"
     CDS             3593..5041
                     /locus_tag="PLH57_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01532.1"
                     /translation="MAAIKLFLRNMLQSRSRIFAAVCLFGFAAQVLIAFCFQSRAWVD
                     EIWVTFNPAYSWIYRVGELDVDWVQGVRSWLAPLLVGSILRVFHALGLTDGQVVLPLM
                     RILLATVTFAAKGYFILTVARKLGLRSLPWLAGLFVFLTPEFLRYSTFGELAALALPL
                     LLVGLVWLDQGSPSKLMAGLCLIALSCALRYPFVVILGWIGLMFLFTRQYRKFQWAFV
                     AGLGFVLFDLLLNSAVNGQWTSPFLHYAYADLIDGKPARDGVTPFYFAFELLWRFMTE
                     PLFVLFFLAVAFSPKKLRPYWGATLSLLLLHTLFGHKEYRYFYPIAVLGAALAGLVLQ
                     YWFETTKWQYRRQVLVLILTLFLSFAAWRGSKKTRWNDYQTPSVLETWVGAQTDVRGL
                     LVYGWNGIYSGGHYTFHRPLPYSYAIDRDHLRRKRLDPHDYNYVVTSRSENPPCKERR
                     IPMRTIFSPVEDEGAVYRCTLDEIHTFLSEEH"
BASE COUNT         1297 a         1315 c         1159 g         1327 t
ORIGIN      
        1 acgatagtcg aatttctcca cgtccgacct gagacgggct ccgaccgtcc acagatgatc
       61 cgcaatatag cggtcacttt tgaccgcgct attaaaagac gatcgatcgc gttggtaaaa
      121 cacataggga tcaacatttt tgaaatactg tcctaaatcc caagaactgt acaaagccca
      181 gagatctttg tcgccacgct cgatggtcga agaatcagac gtgttgtatt ctttgatttt
      241 cgaaaacaat aagtcactcc aacccaaacc atactttgcc cgcgctctca atgcatcgaa
      301 actacgacca tgattgcccc agttcccttg gccgatcacc agcccgtcac cgtatttgag
      361 tgattggcga ccaaaataaa actcactcca acccaagggt tgccaaccga cataggcctc
      421 gtaaacaccg aattcaccgt gggcaccaag cgaactactg ccgcttgttg acacttctgt
      481 gttggcagtt gttgaagaac taatatgagt aggttcccct aaggcctttg caaactgcgg
      541 aacaatcaca agcgaagttt ttgaattagg cgtgacagtc atgccaggac gaacgcgcaa
      601 tagagtataa cgacggttgt tctggtaatc gactcgattg tcggactgat acttcagccg
      661 ggcattccct tcaaacttga tcatcgaagt atcaactgcg tgagccgccc cagaggcgct
      721 catcaacgct acgactccaa gtagtcctaa ctttttcatt cgaaatcccc ccaacgttgt
      781 ttttactatg acgctttttt tgacagtaac tttgttttta agacaattgc tgttttgttc
      841 accatttcgc gcaaaacgcg gtctactttg gtttcctgtt tgtgcaaaat aaaattgcga
      901 atcaatcgaa atgcccggct cggataaaag aaatacgata cagcgtaaaa ctgagccatg
      961 gcgtagagct tataaaaaag tatctggcga tcgctaaaac gaggattcca cgaacgtacc
     1021 gtcaacatgc tggtagccgc agtcatccag tggcagtact cgtcatcgac ttcgattttt
     1081 gaaatcgcgc tagtctcacc aaaaagctgc gttccaggga gtggcacaaa agtagcagtc
     1141 gccacttcat gaactccaat acgcgccatg tggcgcaacc acttgaaagt ggcacgaatg
     1201 tccgattttt cctcgaaggg aaatccgacg ataataaata ggcagacttt catgccgttg
     1261 cgtaaacaag ccttggcaga atcttcgaga ctgtttaaat cgactcgttt ctgaatgcga
     1321 tcgagtattc ttgggctacc cgactccgga gcgaaagtaa tatgttggca tccggaacgc
     1381 atgaggtaac tcgcgcattc gtcgtcgaca acttcagacc gcgtaccggc aggcaaagtc
     1441 caagtgatct ttaattcgcg tcgtaatatt tctttgcaaa aagttagtat ccaatcgcgt
     1501 tttacgattg cagtaagatc ctgaaactga aagtcggcag caccgtactt tttcatgtac
     1561 gactcgatct cgttaactac gtcgatcgga tcacgtggct tccacagttg ggtccacatg
     1621 ttgggcgagg aacaaaaggt acagcggtac gggcatccgc gagtggccaa catcggaata
     1681 gtttttccgt tgctttgggg cccctgataa atctcagctt cgttgtaggc catggggtcc
     1741 atcaaatccc aagctggcca aggaatgtta tctacttctt taatgcgtgg acggcgttca
     1801 ttcttaacaa cttgccccgc ttgactcttg taagccacgc cttgaattgt gcgcgcctgc
     1861 tcaagattgc cggcgcccag caaatcgatt aattctcgaa aggtatactc accctcaccc
     1921 agaacacaga aatcaacagg cgtctgctcg agcacggttg gatgcatcga cgtgatgtgt
     1981 tcgcctccgc aaacaagcca agccttagga aagcgttctt tgagcgcagc caacaaatca
     2041 cggaccattg gccaggcatg cgtgaacata caggtcacac caatcacctg agtgtcactg
     2101 ggaatgcgtt ccaaaatttc ttcaaaactc aaaccttgaa taaggcgttt cttacgtttg
     2161 ggccatggtt ccagttttcg gacggccgca ccgaccgcat cgaccacggt aacctcgtgg
     2221 tggtgacttc gcagatagcc cgcaatgtag gcaatcccta gcgggggcaa cgtaatggtg
     2281 gagtaattat cgaccgcaac taacgccggc ggttgaacaa gggtgacttt cattttggct
     2341 cccttcgcgt cggcagctgc aagacgccca tgaaaaaact cgcaagaatc atattaaaac
     2401 ccaacgtggc ggccaaccct gacggaatca ctattcgcat catttggcgc ggttcaatat
     2461 tagcaaactc ggaatggccc cactgctcaa caccgaccag gagccctacg actccagcca
     2521 agaacaacaa caagcctagg accaaacctt tttcgatcac aaagtgatct aaaaatttct
     2581 gcattcgctt cgaggccggt aacaaacctt tgtgcactgc tgaaatttgg gctaacgcat
     2641 aaaagctaac cgattgaaaa ccgattatca ctgcaatcgc ggaataaaac agcgcgtgaa
     2701 tatctagtcg aatcccacca atgtccagcg ggccagtgac aagcaaactt cccaacagca
     2761 agcccgataa aatgagcgcc aatcccggat aataaaataa ccaacggggc gaaaacagca
     2821 acataaagcg caggtggcgc cagccgtctc gccaagggcg caaatgaggt gctcgcgagc
     2881 gcccatctgg ctgcaaggtg gtcggcactt ccactatgcg catccctttg agcgaagctt
     2941 taatgaccat ttcagaggcg aattccattc cagtggtatg taagtccatt ttttgaaagg
     3001 cgctttttga atacccacgt agtccacagt gaaaatcgcg aactggaatc ttataaagta
     3061 accgcccgat tccactcaaa gcaggatttc cgatgtaccg atttttccaa ggcattgccc
     3121 cttttttaat gcctccggaa aatcgattcc ccataactag atcgtaattt tcgcgcagtt
     3181 tttcgacaaa cggcattagc cccgaaaaat cgtaactatc gtcggagtct cccatgatga
     3241 cgtagctgcc gcgagccgct tgggtggcat gataaagtgc agcgccgtag cccctgaccg
     3301 gaacgcttac tacacgagct ccgcatcgtt cagcgatttg ttgcgaccca tcggtcgatc
     3361 cattgtcgcc aacaacaatt tcgccggcga tctggtgtcg ctccaaaaaa ttcttagctt
     3421 ttttaatgca cgcctcgagc gtttcggctt cattgaggca gggcattaca atcgagagtt
     3481 cgattggcga agatgatgac ataaagacag gggttagcat cgatcaattg attcatcaac
     3541 tccgatatga tgcaatgggc ccatctctca ttaaacatgc ggaaaatgac ttgtggcagc
     3601 aattaaatta tttctgagaa atatgcttca atcacgcagt cgcatctttg cagcggtgtg
     3661 cctttttgga ttcgcagcgc aagttttgat cgcgttttgt tttcaaagcc gcgcctgggt
     3721 cgacgagatt tgggtaacat tcaatccagc ctactcttgg atttaccgcg tcggggaact
     3781 cgatgtcgac tgggttcaag gcgttcgaag ctggttagcc ccacttttgg tcggatccat
     3841 cctgcgtgta ttccatgctc tggggctcac agacggtcag gtggttttgc cacttatgcg
     3901 catcttgctt gccactgtga cctttgcggc taagggttat ttcatcttga ccgtggcacg
     3961 aaaactgggt ttgcgttctc tgccttggtt ggcgggcctt ttcgtttttc ttacgccaga
     4021 atttttacgt tactcaacat ttggcgaact tgcggcgctg gcgcttccgc tactgctcgt
     4081 aggactcgtt tggctcgacc aaggaagccc cagtaaattg atggcgggat tgtgtttaat
     4141 cgcactaagc tgtgcgttac gatacccctt cgtcgtaatt ttgggttgga ttggcctaat
     4201 gtttttattc acccgccaat accgaaaatt tcagtgggct ttcgtcgcgg ggttaggttt
     4261 cgtattattt gatttgctat tgaactctgc ggtcaacggc cagtggacca gcccgttttt
     4321 gcactacgcc tacgctgatt tgatagatgg taagcccgcc cgcgatggag tcacaccgtt
     4381 ttactttgcc ttcgaacttc tctggcgatt catgactgaa ccgctattcg ttttattttt
     4441 tttagcagtc gcattttcac caaaaaaatt acgtccgtat tggggagcca cactttcact
     4501 tttattgctc cacactctat tcggccacaa agaataccgt tatttctacc ccatcgcggt
     4561 tctgggagcg gctctagcgg gcctcgtgtt acaatactgg tttgaaacaa cgaaatggca
     4621 gtatcgccga caagttcttg ttttaatttt aacgctattt ttatcgttcg ccgcctggcg
     4681 tggatctaaa aaaactcgct ggaatgatta tcaaactccc tcagtactcg aaacctgggt
     4741 gggcgcccaa actgacgttc gcggtttact tgtttacgga tggaatggaa tttattcggg
     4801 cggccattac acctttcacc gccccttgcc ctattcctac gccatagacc gtgatcatct
     4861 gcgacgaaaa agacttgatc cgcacgacta caattacgtt gttactagtc gatctgaaaa
     4921 tcctccgtgt aaagaacgcc gcattccgat gcggactatt ttttcgcccg tcgaagatga
     4981 aggtgccgtt tatcgctgca ctctcgatga gattcacacc tttttgagcg aggaacatta
     5041 attggaagac tccaatcaag aacaccgcaa actttggttc gccactcaac tttggact
//
LOCUS       DAOMXO010000077         5085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8757_length_5085_cov_3.731809, whole genome shotgun sequence.
ACCESSION   DAOMXO010000077 DAOMXO010000000
VERSION     DAOMXO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8757_length_5085_cov_3.731809"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLH57_02620"
     CDS             <1..307
                     /locus_tag="PLH57_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRK01533.1"
                     /translation="VEILTRNFRKRFGEIDIVAKEGATICFVEVRSSKRKSGYVRYSV
                     GFQKQFRLRNVIAAYLRSAKKPMSGLSGSSVRFDVAWVCGLEVEYWKNVELAVQSKT"
     gene            268..858
                     /locus_tag="PLH57_02625"
     CDS             268..858
                     /locus_tag="PLH57_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01534.1"
                     /translation="MEKCRARGTIQDVIRKTSFVFALTLLLTVLFAAQPSHALDRRLK
                     LMFKTAGYGAAAGALIGGAVTALGVGDIRNVFMGASSGMYAGILLAAYIVATPPNRDA
                     ERRQNPYSPRRRVGPNDWENEGWEEEDFNKTLDPYVPERSSLDQGPDSSGEHPNDRPV
                     AGSVGRAGGVQLSRAESTALHQSGVSIWMPLVTLGF"
     gene            complement(855..1199)
                     /locus_tag="PLH57_02630"
     CDS             complement(855..1199)
                     /locus_tag="PLH57_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRK01535.1"
                     /translation="MAMKTQVRNTGGKTVISIEGKLDFESSDTFRQWLLGFSRTATSG
                     PVVFDLGALQFVGSSGITQFIESLKDYNRRATIKPVYSNVSSEFRRMMAVYDDSGSFQ
                     FWDTLASPLLDN"
     gene            1232..2164
                     /gene="rsmI"
                     /locus_tag="PLH57_02635"
     CDS             1232..2164
                     /gene="rsmI"
                     /locus_tag="PLH57_02635"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRK01536.1"
                     /translation="MKKKLSEQENVGTLYLVGTPIGNLEDMTYRGVRVLSEVDKIYCE
                     DTRRTSQLLRHYKIEAKAGLESFHDHSSPKILRKIEALFLENQSVAYVTDAGLPGVSD
                     PGFVLVRAALRVGAPVTVVPGPSAASLVFAASNLPSPKYLFHGFFPRSHGEVERTLEL
                     LRQLPVVHIFYEAPGRIMALLEVLKRNVPQAQVVLGRELTKMHEEWIRGECAEVYETL
                     VERQEAEGKIRGEFAVAVHVQALHKLLREEKNAANSTESEPLVQLELTEAQKKEISEK
                     LRSGQSSKDVAKELAKKFRLPKRMIYEFIIRHLT"
     gene            2339..3616
                     /locus_tag="PLH57_02640"
     CDS             2339..3616
                     /locus_tag="PLH57_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRK01537.1"
                     /translation="MKARTLSRKNAAAAAAAIKKSSGLSETPSHDWNLNPSNLEDAIE
                     TIRTLEREIARTIAITQKQVEQLEGLANFSAILNSTLDTDTIRFKSMEAVCNLLNCET
                     GSLLLVDPSTKELYWETALGEGGERLKHSMRLAIDDTSIAGHVAATCKTLVVNDVKLN
                     PYHLKKADSTAGFETKNMVCSPVVSKGELIGVLQAINRIDGNFTNEDAAFLEALSFQV
                     AIAVENANLDSRLKNTFIQTAAAMAEAIEAKDRYTGGHTKRVVQYSLAIGQYVKLSPI
                     EREHLKLAAILHDIGKIGIDDKILKKQYALNDEEYDVMKKHPAIGYEILSQVSGLKEI
                     VDGARYHHERPDGKGYPYGLKGSEIPKVASIISVADTYDAMVSTRPYRKGLDPKVAFE
                     EIVKYRGTQFDAEVVDAFRTAFESGTLKKKIQE"
     gene            3706..4578
                     /gene="accD"
                     /locus_tag="PLH57_02645"
     CDS             3706..4578
                     /gene="accD"
                     /locus_tag="PLH57_02645"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRK01538.1"
                     /translation="MSHWFSQGKSPKIKEKPSEKVVRVPDGFWVKCSSCGEILQADAL
                     KENLGVCPECSFHFRMGAWDRIALLCGDEFEEFDSKLVSIDPLDFTDSRSYAERLESA
                     QKSTQLSDAIIAGKGSIHDVDTILGVFEFRFMGGSMGAVVGEKVSRCFERALDEKLPC
                     VLITASGGARMQEGILSLMQMAKTSALAGKMKKEGLAYLSVLTDPTTGGVAASFAMLG
                     DVILAEPGALIGFAGPRVIESTMRQSLPPGFQRAEFLVKHGFLDAVVSRKDLSQTIAR
                     LLGFLHRRTWVKGS"
     gene            4596..>5085
                     /locus_tag="PLH57_02650"
     CDS             4596..>5085
                     /locus_tag="PLH57_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01539.1"
                     /translation="MALGWVLTASALHAQDAGSRTRLSFSGKTLHLSCQKSIRDLNTS
                     RLECYGNVYLRRPGELLTADYAWIDLDTEQMHAEGNVVYFTPTNVIYGAAMDFNFLNE
                     TGVIRDGRIESEKYQLLGDVIERRGEGSFRAQDGEYTTCRDCPASWKLAGRTVDLEVE
                     QYA"
BASE COUNT         1330 a         1172 c         1395 g         1188 t
ORIGIN      
        1 ggtggaaatt ctcacccgta actttcgtaa acgattcggt gaaatagaca tagttgcaaa
       61 agagggtgcg acgatttgtt tcgtcgaagt gcgctcaagc aaacgcaagt ctgggtatgt
      121 tcgttacagc gtcggttttc aaaaacagtt tcggcttcgc aacgtgattg cagcgtactt
      181 gcggtcggca aaaaaaccaa tgtcgggcct ctcgggcagt tccgttcgct tcgatgtggc
      241 ttgggtttgt ggtttggagg tcgaatattg gaaaaatgta gagctcgcgg tacaatccaa
      301 gacgtgattc gaaaaactag ttttgttttc gctctaaccc tattgttaac cgtgctgttt
      361 gcagcgcagc ccagccatgc gctggaccga cgtttaaaac ttatgtttaa gacggctggc
      421 tacggcgcgg cggctggggc cctcatcgga ggggcggtaa ctgcgttagg cgtgggcgat
      481 attcgcaacg tattcatggg ggcttcgagc ggaatgtacg ctggcatatt gctcgccgcc
      541 tacatagtgg ccacgcctcc taatcgcgat gcggagcggc gacaaaaccc ttattctccg
      601 aggcggcgcg tggggccaaa cgattgggaa aatgaaggtt gggaggaaga ggattttaac
      661 aaaacccttg atccctatgt accggaacgt tcctcgctcg atcaggggcc agacagctcg
      721 ggagagcatc caaacgatcg tcctgtggcc ggctccgtgg gaagagcagg tggagtgcag
      781 ttgtcgcggg ccgaatcgac ggcgctgcac cagtcggggg tttcgatttg gatgccgctt
      841 gtaacgctgg gcttttagtt gtcgagtagt gggctagcca aggtgtccca gaactgaaaa
      901 cttccggaat catcatacac tgccatcatg cggcgaaact cgctactgac gttggagtag
      961 acaggtttaa tcgtggcacg gcgattgtag tcttttagcg actcgatgaa ttgggtaatt
     1021 ccgctggagc ctacgaactg aagcgcgccg aggtcgaaaa ccacggggcc cgaggtggcc
     1081 gtgcgggaga atcccagtaa ccactgccga aaagtgtcgc ttgactcgaa gtcgagtttc
     1141 ccttcgattg agattaccgt ttttccacca gtgtttcgaa cttgagtttt cattgccatg
     1201 gcgagccccc ttggcctaat gatagtaaac gatgaaaaaa aaattaagcg agcaagaaaa
     1261 cgtaggcacg ctttacctag tcggtacacc gatcgggaac ctcgaagata tgacctatcg
     1321 cggggtgagg gtgctctcgg aagtagacaa aatctattgc gaagatacgc gacgaacgag
     1381 ccaactactt cgccattata agatcgaggc caaagcaggg ttggagagtt ttcacgatca
     1441 ttcttcgccc aaaattctta gaaaaattga ggcactcttt ctagaaaatc aaagtgtcgc
     1501 ttacgtgacc gacgcagggt tgcccggagt gagcgacccc gggtttgtgc tcgtgcgtgc
     1561 tgcgttgcga gtaggagctc cggtaaccgt ggtgcccggt ccgagtgccg cgagtttagt
     1621 gtttgccgcg agcaatttgc cgtcgcccaa gtacttgttt cacggctttt tcccgcgttc
     1681 acacggtgaa gtggaacgaa cacttgaact tttacgccag ttaccggttg ttcacatttt
     1741 ctacgaagcg ccgggccgga tcatggcttt acttgaagta ctaaagcgca atgtgccgca
     1801 agcccaggtc gtgctgggtc gtgaacttac gaaaatgcat gaagagtgga ttcgtggaga
     1861 gtgtgccgaa gtttatgaaa ctctcgtcga acgccaggag gccgaaggca aaattcgtgg
     1921 tgaattcgcc gtggcggtac atgttcaagc attgcataag ctgctacgag aggaaaaaaa
     1981 cgccgcgaat tcaacagagt ccgagccact tgttcagcta gaactcacag aagcccagaa
     2041 aaaagagata tctgaaaaac tgcgctcagg gcagtcctcc aaggacgtcg ctaaagagct
     2101 ggcaaaaaaa ttccgccttc ccaaacgaat gatttacgaa tttatcatcc gtcacttgac
     2161 ttgagccccg tgtcccttaa gctagaaagt gtggttgtgg agctctagaa tccatggatg
     2221 ggttcgaaac gggtgaaaag gattttcgca aaaagttccg cagaggccag attcgggggc
     2281 ccaaggatgg gatcctgaat ctggcttcag tattttaagc agaaaaaaac gaaacactat
     2341 gaaagcacgg acgctctcta ggaaaaacgc cgcggctgcg gcggcagcaa tcaaaaagag
     2401 ttctggtctt agtgagacgc catctcacga ctggaatcta aatccctcta atttagaaga
     2461 tgcgattgaa acaattcgta ctttagagcg cgaaatcgcg cgaacgatcg ctattaccca
     2521 aaagcaagtc gagcaactag aagggctcgc caatttcagc gcgattttga attctaccct
     2581 cgataccgac acgattcgtt ttaaatcaat ggaggcagtt tgcaatttac tcaattgtga
     2641 aactggctcg ctgttgttgg tggatcccag cactaaagag ctatattggg aaaccgcatt
     2701 gggcgaaggc ggggaacgtc tcaaacattc aatgcgtctg gcgatcgatg atacgagtat
     2761 tgcggggcac gtggcggcta cctgtaaaac gctcgtcgtt aacgacgtaa aacttaatcc
     2821 atatcacctt aaaaaagctg attccacggc ggggttcgaa actaaaaaca tggtatgttc
     2881 cccggtggtc tccaaggggg agttgatagg tgtacttcag gcgatcaatc gaattgacgg
     2941 caattttacc aacgaagacg ccgctttcct ggaagccctg tcgtttcagg tcgcaatcgc
     3001 agtggaaaac gctaatttag atagtcggtt aaaaaacact ttcattcaaa cggccgcggc
     3061 gatggcagag gcaatcgagg ccaaagatcg gtatactggg gggcacacca aacgagtggt
     3121 gcagtactcg ctagcgatcg ggcaatacgt aaagctttcg ccgatcgagc gtgaacattt
     3181 aaaattagcc gcgattttac acgacattgg caaaatcgga atcgatgaca aaattctaaa
     3241 aaaacaatac gccctgaatg atgaagaata cgatgtgatg aaaaaacacc cggcaattgg
     3301 ctacgaaata ttgagccagg tttcggggtt gaaagaaatt gttgacggag cgcgctatca
     3361 tcacgagcga cccgacggca aaggctatcc ctatggccta aaagggagcg aaataccaaa
     3421 agttgcgagt attataagtg tcgctgacac ctacgatgcg atggtgagta cccgtcccta
     3481 ccgaaaaggg ctggacccca aagtcgcctt cgaggaaatt gtaaaatacc gaggcaccca
     3541 gttcgatgct gaagtggtcg acgcctttag aaccgctttt gagagcggga ccctcaagaa
     3601 aaaaatacag gaataacaaa ggtttttagc ccatccggag tttgacgacc cccccagttc
     3661 gagggtacgc tcgactcatt attttaccgg agaggcacga attacgtgag tcattggttt
     3721 agccaaggta agtccccaaa aattaaagaa aagccgtcgg aaaaagtcgt gcgagtccca
     3781 gacgggttct gggtcaagtg ctcgagttgc ggcgaaattc ttcaggcaga cgcgctcaag
     3841 gaaaatctcg gcgtatgtcc agagtgttcc tttcattttc gcatgggagc ttgggaccgg
     3901 atcgcgctct tgtgtggaga tgagtttgag gaattcgata gtaaactggt ctcgatcgat
     3961 ccgttggatt ttaccgatag tcggtcttac gccgagcgtt tagaaagcgc acagaaatca
     4021 acccaactct ccgacgcgat tattgccggc aaagggagca ttcacgacgt tgacaccatt
     4081 cttggtgtat ttgaatttcg cttcatgggc gggagtatgg gggcggttgt tggcgaaaaa
     4141 gtctcgcgat gtttcgaacg agctctcgat gaaaagctgc cctgtgtttt aattaccgcg
     4201 agcggtgggg cgcgaatgca agaagggatt ctatccttaa tgcaaatggc aaaaacttca
     4261 gctctggcag gtaagatgaa aaaagaaggt ttagcctatc tttcggtgtt gaccgatcct
     4321 accactggtg gcgtggcggc tagctttgcc atgttgggcg acgtaattct cgccgaaccg
     4381 ggggcgttga tcgggttcgc cgggccacgg gttatagaat ccaccatgcg tcaaagtctg
     4441 ccacctggat ttcagcgcgc ggaatttttg gtcaagcacg gatttttaga tgcagtcgtc
     4501 agtagaaaag atttaagtca aaccatcgca agacttctcg gatttcttca tcggcgaaca
     4561 tgggtaaaag gctcttagct ctagcatggc ctttaatggc cctcggttgg gttttaaccg
     4621 cgagcgcgct tcacgcccaa gatgccggtt cacgtactcg tctttcgttt tccgggaaaa
     4681 cactacattt aagctgtcaa aaaagtatac gtgatctcaa cacgagtcga ctcgagtgtt
     4741 acgggaacgt ttatttaaga cggccaggcg agttgttaac ggccgattat gcgtggatcg
     4801 atctcgacac tgaacaaatg cacgccgaag gaaacgtagt ttattttact ccgaccaacg
     4861 tgatctacgg cgccgcgatg gactttaatt ttctcaatga aacgggcgtc attcgcgacg
     4921 gtcgaattga gagtgaaaag taccaactcc tcggtgatgt aatcgaaagg cgcggtgagg
     4981 ggagctttcg cgctcaagac ggagaataca cgacctgtcg cgactgcccg gcgagttgga
     5041 agttggcagg tcgcaccgtg gacttagaag tagaacagta cgccc
//
LOCUS       DAOMXO010000078         5055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8832_length_5055_cov_4.631000, whole genome shotgun sequence.
ACCESSION   DAOMXO010000078 DAOMXO010000000
VERSION     DAOMXO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8832_length_5055_cov_4.631000"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLH57_02655"
     CDS             complement(<1..793)
                     /locus_tag="PLH57_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HRK01540.1"
                     /translation="MIKVSLEDVERARTVLRKLLKPTPLLFNPWLSEKFRCEVYLKHE
                     NLQPIGSFKIRGATYKISNLAPRKRKQGVFAASAGNHAQGVAWGASRFRIRSTIVMPS
                     SAPLMKIEQTQALGAKILLKGDNYDEAYEEANRLAKKTGAPFVHPFDDPDVIAGQGTV
                     GLELMDQIPDVDVVIGSMGGGGLMAGVGVVMKALRPQVWMVGCQASGARSLLESVKKN
                     RVVNTGKVETFADGIKTLHARPNLIHLLRPLLNQLDHVDDESIATA"
     gene            complement(790..1605)
                     /locus_tag="PLH57_02660"
     CDS             complement(790..1605)
                     /locus_tag="PLH57_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRK01541.1"
                     /translation="MGNKLIITCAITGAETTRDQNPNLPITPDEQATAAYDCWKAGAS
                     IIHLHVRDDSGKPSQSVDRFREVIEKIRKKCPVIVQVSTGGAVGEKIERRAEPLTLKP
                     EMASLNTGSINFGDEVFLNLPSDVEFLAKRMSELSIKPEIEVYDYGMFEYSLKLVKKG
                     IIKQPAHFQFVLGTGHGISGEPRNLCRMADLVPEHCTWTAAGVGRYQLPVAAQAIVMG
                     GHVRVGLEDNIYFSKGVLAKSNAQLVQRIANLAEILQRSVATVDETKEILGLK"
     gene            1719..3149
                     /locus_tag="PLH57_02665"
     CDS             1719..3149
                     /locus_tag="PLH57_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01542.1"
                     /translation="MPFSKRLESVVIYSVNFVALYFCVPGFLPKEVRNYFIQLFTTDR
                     GHGLLFMFASSIPLLLAAARIKRRGYTRTIVGACFCLLTIAMTFDLLADAILGHVVSN
                     WLLRALWVFLFIFGIAAREDLLSSFKLRLRDIKRSTFLARIVLFLGLFFLLRQSFAFF
                     YNRGGPFADEINFWHTAATDFLSGGFATVIQKHSYTLAVPWLASLPVSLFGGSHSEHV
                     YAYSTAIVFGSIFLVVELARSPRALLAGLGVLLLCFAIQRDLFSLYGASLYGEGLASL
                     LCATVLLEFLRLYYLKRPTPKQIFAFSLIAAFAAVSKPPLAYLVWMLPVVLATRSRSW
                     PSRALLLFSGILFVPWRLWLALLNKSTYYQSFDLLNALDRRVAFEIPIKMISGLLDHG
                     TPQSAYTIALIVAVVLAILKKSTELLRVYALLIFVYWGFVFALYATLWQHMEQFSAGR
                     YISHAAIACVLMIPLAFSKTINEETK"
     gene            3146..3955
                     /locus_tag="PLH57_02670"
     CDS             3146..3955
                     /locus_tag="PLH57_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK01543.1"
                     /translation="MKTLNVIIPYFNEAQRMEPMIVAMAQEIAANDIFDEAGLPLRFI
                     WVDDGSTDNGRMILETLITKHLPNDSRLGAFQVHHEFVDLGKNSGKASAMHAGIQRAK
                     LITTKNGESAIVSFWDADGELLADGLFSLIYEVYSEKADIAFGSRFNKRNPQVLNFRH
                     YLGNKSLTVLSNWFSDLNLSDVHCCARAIRIELLAQIPLKSKGFDFEAEFVGLIGRIS
                     SPMLKLTEVPLTYLPRTREEGKKIGIRHVLPQIWQAIRCRYFVKNIELRNF"
     gene            complement(3957..4811)
                     /locus_tag="PLH57_02675"
     CDS             complement(3957..4811)
                     /locus_tag="PLH57_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRK01544.1"
                     /translation="MQAGSPEDISGYEQKSSWDVAVRYQDLKKFGHIPDKEKQRLKAR
                     AIQGVIAQAQAVVGPVWRPQVRKTIDISELRSAGVDGELNLSETLEAAYGSGDKSLLA
                     ANSLRFDAALEKNTTVALVMDASLSMTGEKIALLAVAAAVVALSVPSEHLVLMGFDSK
                     VRWIKKYGNKMSLEKIVEGVLELPAGGFTNLEIALEEISAVLNAPKLRRANVILITDG
                     KYTEGRDPTSLARRFKRLNVLKIGRDQAGRGLLKDLVRLGNGSFFEARKMNDLPKTMY
                     AALRTLLR"
BASE COUNT         1195 a         1215 c         1120 g         1525 t
ORIGIN      
        1 ttgcagttgc gatcgattca tcgtccacgt gatcgagctg attcaaaaga ggacgcagca
       61 aatggattag attcggacga gcgtgcagtg ttttaattcc gtcagcgaag gtttcaactt
      121 ttccagtatt aaccactcga ttttttttga cggactctaa aagtgatcgt gctcccgacg
      181 cttggcagcc gaccatccat acctgtggac gcaacgcttt cataaccacg ccaacccctg
      241 ccataaggcc gccgcccccc atcgatccaa taactacgtc gacatcaggg atttgatcca
      301 tgagctctaa acccacggtt ccctggcctg ctattacgtc cggatcatcg aacggatgaa
      361 caaatggtgc gcccgttttc ttggcgagcc tatttgcttc ttcataggcc tcatcgtagt
      421 tgtctccttt gagcaatatt tttgctccca gcgcctgagt ttgttcaatt ttcatgagtg
      481 gggccgacga tggcattaca atggtcgaac gaattcgaaa tcgactggcg ccccaagcta
      541 caccctgagc gtgatttccg gcacttgccg caaatactcc ctgtttgcgt tttcgaggag
      601 caagattaga tatcttgtag gtcgcacctc gaattttaaa agaaccgatt ggttgcaagt
      661 tctcgtgctt gaggtaaact tcacaacgaa atttttctga taaccatggg ttgaagagca
      721 agggtgtagg cttcaacagc tttctcaaaa ccgtgcgtgc ccgctctaca tcttcaagcg
      781 aaactttaat catttaagtc ctaatatttc ttttgtttca tctactgtcg cgacagatcg
      841 ttgcaaaatt tctgctaaat ttgcgatccg ctgcaccagt tgagcgttgc ttttggccaa
      901 cacaccttta ctgaaataaa tattgtcttc caagccaact cgtacgtgcc cacccataac
      961 aatcgcctgt gccgcaactg gaagttgata tcgcccgacg cctgcggccg tccaggtaca
     1021 atgttctgga actagatccg ccattcgaca gagattgcgt ggctcaccgc tgataccgtg
     1081 accagttccc aacacgaact gaaaatgcgc gggttgcttg ataattccct tttttaccag
     1141 tttgagggaa tattcgaaca tcccgtaatc gtaaacttca atttcaggct taatagagag
     1201 ctcgctcatg cgtttggcga gaaattccac gtcgctaggg agattcaaaa aaacttcatc
     1261 tccgaaattt atggaaccgg tattaagact ggccatttcg ggtttaagcg taagtggttc
     1321 ggcgcgccgt tcaatttttt caccgacggc accaccggtg gaaacctgaa caatgactgg
     1381 gcattttttc ctgatttttt ctattacctc tcgaaaacga tccaccgact gactcggttt
     1441 cccggaatcg tctcgtacgt gtaaatgaat gatggaagcg cctgctttcc agcaatcgta
     1501 tgctgctgtg gcctgttcat cgggggtgat agggagattt ggattttggt cgcgagtcgt
     1561 ttccgcgccc gtaatagcgc aggtaatgat cagtttattg cccatattta caagacatat
     1621 cagcaccaga aatagccgta aactatgaag aaaccctgcg ctacaattgt gcgcttaaat
     1681 tcgcagtcat tttgaataga gtttgttagt ggtgttctat gccgtttagc aagcgactcg
     1741 aatcagtagt aatttactcg gttaattttg tagcccttta cttctgtgta cccggttttt
     1801 tacccaaaga agttcggaac tatttcattc agctttttac caccgaccgt ggtcacggat
     1861 tgttgtttat gtttgccagc tcgattcccc tgcttttggc ggccgctcga atcaaacgac
     1921 ggggctatac ccgcacgatc gtaggcgcct gcttctgcct tctcacaatt gccatgacgt
     1981 ttgacctgtt agccgacgca attttaggtc acgttgtgtc caattggctg ttgcgcgcac
     2041 tgtgggtctt cctatttatt ttcgggatag ccgcgcgcga ggacctgcta agttctttca
     2101 agctccgatt acgtgacatt aagaggtcca cctttctcgc acggatagtg ctttttttgg
     2161 gtttattttt tttactgcgt cagtcgtttg ctttttttta taataggggt gggccgttcg
     2221 ctgatgaaat taacttttgg cacactgcag ctaccgactt cctgtcgggg ggattcgcca
     2281 ctgtaattca aaaacatagc tacacattag cggtgccgtg gttggcgtca ttacccgtta
     2341 gtctctttgg tggttcacac tcggaacacg tatacgccta ttcgactgct atcgtttttg
     2401 gatcgatttt tttagtagtc gaacttgctc gcagtccgcg cgctcttctt gccggattag
     2461 gagtcttgct gctttgtttt gcgattcagc gagatttatt ttcactctat ggggcctcgc
     2521 tttacggcga gggtttagct agcctattgt gcgcaacggt tttattggag tttttgagac
     2581 tttattattt aaagcgtccg acaccgaaac aaatttttgc cttcagttta attgcagcat
     2641 ttgccgccgt ttcgaagcca ccactggcct acctagtctg gatgttgccc gtagttttag
     2701 ctacccgctc ccggagctgg ccatcacgag cattgttact tttttcggga atcctattcg
     2761 ttccgtggag actttggttg gcgctactga acaagagcac atattatcaa tcctttgatc
     2821 tattaaatgc tctcgatcgt cgggtcgctt ttgaaattcc tattaaaatg atttcgggtt
     2881 tacttgacca tggaacaccg caatcagcct acactattgc gttaattgtg gctgtagttc
     2941 ttgcgatcct gaaaaaaagt actgaactct tgcgagtgta tgcacttttg attttcgtat
     3001 attggggctt tgttttcgcg ctgtatgcga cactttggca gcatatggaa cagtttagcg
     3061 ccggcagata tatatcacac gccgccatcg cttgtgtttt aatgatcccg ctagcttttt
     3121 caaagacaat taatgaagag acgaaatgaa aacccttaat gtgattatcc cgtatttcaa
     3181 tgaagcccag agaatggagc ccatgattgt cgcaatggcc caagaaattg cggccaacga
     3241 catttttgac gaggctggtt tgccattgcg tttcatatgg gtcgacgacg gatctaccga
     3301 caatgggcgg atgatcctcg aaacattaat tactaaacac ctacctaatg attcgcgtct
     3361 cggggcattc caagtccatc atgaatttgt agacttgggt aaaaactccg gaaaagcttc
     3421 agcgatgcac gccggaattc aacgcgccaa attgataaca acgaaaaatg gagagagcgc
     3481 tatcgtatct ttctgggatg cggacggcga actgttagct gacgggctat tttccttaat
     3541 ttatgaggtc tactcggaaa aggcggacat tgcgtttggt tcgcgcttta acaaacgcaa
     3601 tcctcaggtc ttgaactttc ggcactatct cggaaataaa tcattaaccg ttctttcaaa
     3661 ctggttttca gacttaaatc tttccgatgt acattgctgc gcacgggcga ttcgcattga
     3721 attactcgcc cagattccat tgaaatcaaa gggctttgat tttgaagcgg agtttgtggg
     3781 gttaatcgga cgaatctcat caccaatgct aaaacttacc gaagtaccat taacttactt
     3841 gccacgcacg cgtgaagaag gaaaaaaaat tggtatacgc cacgtacttc cccaaatatg
     3901 gcaagcaata cggtgtcgct attttgtgaa aaacatcgag cttcgaaatt tttaatttaa
     3961 cgcagtaacg ttcgcaaagc tgcgtacatg gttttaggta aatcgttcat ttttcgagct
     4021 tcgaaaaatg aaccatttcc caggcgtacc agatctttta aaagtccccg ccctgcctga
     4081 tcgcgaccaa ttttcagtac atttagtctt ttaaaccttc tggccaaaga agtgggatct
     4141 cggccctcgg tgtatttgcc gtcggtgatg agtattacat ttgcccgtct taacttcggt
     4201 gcgtttaata ctgcgctaat ttcttcaagt gcaatttcaa ggttggtaaa accgcctgcc
     4261 ggaagctcca acaccccttc gacgattttt tccaacgaca ttttattccc gtactttttt
     4321 atccaacgta ccttggagtc aaaacccatt aatacgagat gctcggacgg tacgcttagt
     4381 gcgacgaccg ctgcggctac ggcgagaagg gctattttct caccggtcat gcttaagctt
     4441 gcgtccatta ctaatgcgac cgttgtattt ttctcaagtg cggcatcaaa acgcagacta
     4501 ttggctgcca gaagactttt gtcaccgctt ccataggcgg cctctagggt ttcactgagg
     4561 ttgagttccc cgtcgacgcc cgcgctacgc aattcactta tatcgattgt ttttcgcacc
     4621 tggggtcgcc acactggccc cacgactgcc tgagcttgag caattactcc ttgaatagct
     4681 ctagctttaa gccgctgctt ttctttgtct ggaatatgtc caaatttctt tagatcctgg
     4741 taccgaactg ctacatccca agacgatttc tgctcatacc ccgaaatgtc ttcgggagag
     4801 cccgcttgca atagctcggg agctacaaag atctctagcg tgtttctttt gtcatcaggt
     4861 cgagacgaat ttactaggac ttcatctatt tttaatgggt cattgaagga ttcaggggaa
     4921 tgttggagtt tttttttagt tcttcgaaca gttcacgtaa cactgtatcg cactccactt
     4981 cgaattcttt ttgattctcg gaggcgctta attcgatccg cgtaggaaaa gcgactttta
     5041 ttgcactcca taaat
//
LOCUS       DAOMXO010000079         5036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_8871_length_5036_cov_5.381650, whole genome shotgun sequence.
ACCESSION   DAOMXO010000079 DAOMXO010000000
VERSION     DAOMXO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8871_length_5036_cov_5.381650"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLH57_02680"
     CDS             <1..623
                     /locus_tag="PLH57_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01545.1"
                     /translation="DYSMLVDAETNKKLGLVEVPDSSKTHSPCLWFAVSEQIYDCTQG
                     YLRRGAVDQESLTMIASQNGIEHARITGLIGADARSWYVEVKGCIRVEGCAVDHQVGY
                     VRVNKRTGAATEFFATTAIESQDMAWFEEQLGASGRLSVIAIPEAMGTIAVYETKNAE
                     SYEPVLSKVVDPWLTLWMRRNAWGPHLGIADRASKYVLRNSESRDR"
     gene            complement(644..2161)
                     /locus_tag="PLH57_02685"
     CDS             complement(644..2161)
                     /locus_tag="PLH57_02685"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRK01546.1"
                     /translation="MLKFSLALGIFVLTLVFSGCGGGVSIGAGNNAQNAINDFLASLP
                     KVVWGAQIGSVTTPSGGDSSNLDGCMAVTGDDAGNIYCAGETHGSLGEATAGGPDAYL
                     AKFNATGQLEWLTQLGAVTKKTGRSNAGAETVKGITLDTAGNIYITGATNGSMGEGNG
                     GGYDIFVAKFTSTGALSNIFQLGDATRLFFGLSNAGDDTGYGIKVDASQNIFIGGSTT
                     GSMGETNAGQTDVVLVKLNSAGVLQWIKQLGTTTVVAPGGYSNSSYDVCTGLAMNAAG
                     DLYCGGYTASAMADNPGGLYDMLIVKWSSAGVVQMIKQLGRTNTFGANDNLGQDICRS
                     IAVDADGYVYCGGSTTGNFGEARANSDGSWDIAVVKLSSVGNIEWATQLGANTKLLFG
                     NNAGSDNCYALTLDSGGNVYCAGATSGALGETNAGSLDLVVVRLNSAGVLKSGIQLGA
                     NTRLSGQDNSSMEFCSSVYATGLDSVYCAGQTSGSQFEPNGGNNDAILTKLSLGK"
     gene            complement(2299..2373)
                     /locus_tag="PLH57_02690"
     tRNA            complement(2299..2373)
                     /locus_tag="PLH57_02690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2339..2341),aa:Val,seq:gac)
     gene            2465..3136
                     /locus_tag="PLH57_02695"
     CDS             2465..3136
                     /locus_tag="PLH57_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01547.1"
                     /translation="MSMDPTRASKSCALSYQEALARQTAASGPGWLLFTPPPVLTVTR
                     RSSPGELRVSSNLLNEKNIELLTVDRGGRTTYHGPGQIVGFPLGTLADFVGDSRGVRK
                     FIEKLVSALEWFVESEWRRSGDRRTIDRKKAAQCPGVWARTETGELQKIASIGLAFHR
                     GRMAHGFALNVDETSTAPFQWIHPCGEPDTQMSCVYPRAAREIPAVSARLRLTLASAF
                     EKAIG"
     gene            3210..3908
                     /locus_tag="PLH57_02700"
     CDS             3210..3908
                     /locus_tag="PLH57_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRK01548.1"
                     /translation="MSLLGLEGFVYLMRWLHYFFGVIWIGLLYYFNFVQGSFFAETDA
                     NTKSAAIQKLVPRALWWFRWGAMGTFVTGLTILAIRGHQGGFQMFASSWGVSILTGVA
                     MGTIMWANVWFVIWPSQQIVIKSAQETAAGRAALPEAPGAALRAGVASRTNTMLSIPM
                     LFFMGAASHLPLAVTTASLWPLVFVIAAVLGAIEFNALKGKTGPMTTVKGVIGCGFGV
                     CAGLYIIIEILTKI"
     gene            3971..4999
                     /locus_tag="PLH57_02705"
     CDS             3971..4999
                     /locus_tag="PLH57_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01549.1"
                     /translation="MRGSFYHFNLVKAVLGVVTLWATLFCAIRAEAAKDYPGNDAYLP
                     SSWTCRTLLNERLDALTRNPRIEPALRMRWQNSPDTFALVEAYANKAVVEAIQFGAQL
                     FERPYERIASDRIVVSWNAPMAARFGPDLRHLELFTAEENNYWFDRFEAKAYRVPRGG
                     RGALAHRIGYRALPTPESKKVFIFEVRIKKIDRHVAYRLFRDFAYSLHDQVLVPRPTL
                     YKDEFFGDLAENRIWIVLHARGKELRRQFAYWGDLLSELPNIINVQYHSMTVEHLENL
                     VRERVMMGDENSGIPSGDATNDHRTDSEVRFSLLELDETPQARKESADRMRTISEYEI
                     RVSLIELD"
BASE COUNT         1191 a         1273 c         1357 g         1215 t
ORIGIN      
        1 cagattattc gatgttggta gacgccgaga cgaataaaaa gcttggtctc gtcgaagtgc
       61 ccgattcttc taaaacgcat agtccctgtt tgtggtttgc agtatccgaa cagatttatg
      121 attgcactca aggatatcta cggcgcggag ccgtcgatca agagtcgctc acaatgattg
      181 cttcgcaaaa cggcattgaa catgcccgga tcactggttt gatcggagcc gacgctcgtt
      241 cttggtacgt ggaagtcaaa ggatgtattc gtgttgaagg ttgcgccgtg gatcatcaag
      301 tgggttatgt tcgagtgaac aagcgaactg gagcggccac tgaatttttc gccaccactg
      361 cgattgaaag ccaagatatg gcatggttcg aggagcagct cggagcaagc ggtcgattga
      421 gtgttatcgc aatacccgaa gccatgggta cgattgccgt atacgaaact aaaaatgcgg
      481 agtcgtatga gcctgttttg agcaaagtgg tggacccgtg gttaactctt tggatgcgtc
      541 gcaatgcctg gggaccgcat ctggggatcg ccgaccgcgc aagtaagtac gtattgcgaa
      601 attcagaaag ccgcgatcgc taacaaagcc ccagcgtctg cgtttacttt cctaaactta
      661 gtttcgttaa aatcgcgtcg ttgtttccgc cattgggttc gaattggctt ccgctcgtct
      721 ggccagcaca atacactgaa tctaaaccgg tggcataaac gctactacag aactccatgc
      781 tagagttgtc ctggcccgaa agacgggtgt tcgcgcccag ttgaattcca gatttgagta
      841 ccccagccga atttaggcga acgacaacaa ggtctaggga tcccgcattt gtttcgccca
      901 aagcgcccga agtggcgccc gcacagtaca cattgccacc ggaatcaagc gtcagtgcgt
      961 agcagttgtc gctgccagcg ttgttgccaa aaagaagttt agtgtttgca cctaactgtg
     1021 tggcccactc gatgttgccg actgaactta acttcacaac ggcgatgtcc caagacccgt
     1081 cgctgttggc acgagcttct ccgaagtttc cagtggtgga gccgccacaa taaacataac
     1141 cgtccgcatc cactgcgatg ctacggcaaa tgtcctggcc aagattgtca ttggctccaa
     1201 aagtattggt gcgcccgagt tgcttgatca tctgaaccac gccagccgaa ctccatttga
     1261 cgattaacat gtcatagagt cctcccggat tgtcggccat cgcacttgcg gtgtagccac
     1321 cgcagtagag atctccggcg gcgttcatgg caagacctgt gcaaacgtcg tagcttgagt
     1381 tcgaatatcc gcccggtgcg accacggtgg ttgtaccgag ttgtttgatc cattgaagaa
     1441 cgccggctga gttcaacttc acgagcacga cgtcggtttg ccccgcattg gtttcaccca
     1501 tcgatcccgt ggtggagcca ccgatgaaga tgttttgact agcgtcgacc ttaatgccgt
     1561 aacccgtgtc gtcacccgcg ttgcttagtc caaagaacaa gcgcgtagcg tcaccgagtt
     1621 gaaaaatatt tgataacgcg ccagttgagg taaatttagc gacgaaaatg tcatagccgc
     1681 cgccgttgcc ttcgcccatc gaaccattgg tggcgccagt gatgtaaatg ttcccagcgg
     1741 tatcaagagt aattcccttc accgtttctg cgccagcgtt actgcgaccc gtttttttcg
     1801 tcacagctcc gagttgggtc aaccattcga gttgtccagt ggcgttaaac tttgcgagat
     1861 aggcgtcagg tcccccggct gtagcttctc ccagactccc atgggtttcg ccggcgcaat
     1921 aaatgtttcc cgcatcgtcg cccgtgacag ccatgcaccc gtcaaggttg ctgctgtcgc
     1981 caccactagg tgtggtgacg gaccctattt gagcgcccca gacgactttc ggtaaagagg
     2041 ccaaaaaatc gttgatggca ttctgggcgt tgttgccggc gcctatgctg acaccgccgc
     2101 cgcagccgga aaagaccagc gtcaatacga aaattccaag agctaggcta aatttcaaca
     2161 tggggagcct ccagaaaaat aaggccgtga ttgaaccaga gggactcagg cactccgtcc
     2221 cctcactaga aaagtagcaa ctcggagatt gaaacaaaag tccccgtcga aaaaaattag
     2281 gttttaactg aaaaaaaatg gttggctcga ctgggattga accagcgacc cctaccatgt
     2341 caaggtagtg ctctcccgct gagctacgag ccaacgttag ggaatagaac tttctcttct
     2401 tagcccaggc cgaaccggga ttcaagcttc cgaatttacg ccccggggga gccgtgttaa
     2461 ttcgatgagc atggacccaa cccgcgcgtc gaaaagttgc gctttaagtt atcaggaagc
     2521 tctagcccgg caaaccgctg ctagcgggcc cgggtggctg ctgtttacgc cacctccagt
     2581 gctaaccgtc acgcgaagat ctagccccgg tgaactgcga gtgagcagta acttattgaa
     2641 cgaaaaaaat atcgagttac ttacagtgga ccgcgggggg cgcaccacct accatgggcc
     2701 gggtcaaatc gtagggtttc ccctaggaac actcgccgac ttcgtgggtg attcgcgagg
     2761 agttcgaaaa tttattgaaa agctcgtgtc tgcgctggag tggttcgtgg aatcagaatg
     2821 gcgacgtagt ggggaccgac gcaccattga tcgcaaaaag gcggcgcagt gtcccggcgt
     2881 gtgggctcgc accgaaacag gggaactcca gaaaatagct tcgataggac tggcgtttca
     2941 ccgcggacga atggctcacg gatttgcgct caatgttgac gaaacctcga cggcgccatt
     3001 tcagtggatt cacccttgcg gcgagcccga cacccagatg tcgtgtgttt acccgcgtgc
     3061 cgctcgcgag ataccggcag taagcgcgcg gttaaggtta acactagcga gcgcattcga
     3121 aaaagctatc ggttgacaat tgcggtccag ggtacgaaac tcagagcaca ttaaattttt
     3181 aaaattgacc ttattggagg cacataaaga tgagtctact tggacttgaa ggttttgttt
     3241 atctcatgcg ctggttgcat tacttcttcg gcgtaatctg gatcgggcta ttgtattact
     3301 ttaactttgt gcaaggcagt tttttcgccg aaactgatgc caacactaaa tcggctgcga
     3361 tacagaaatt ggtgcctcgg gcactgtggt ggtttcgctg gggcgcgatg gggactttcg
     3421 ttacgggtct cacgattctt gcgattcgcg gtcaccaagg cgggtttcaa atgtttgcct
     3481 cttcctgggg cgtttcaatt ctaacgggcg ttgccatggg cacgattatg tgggcgaacg
     3541 tttggttcgt gatctggcca agtcagcaga tcgttattaa gtcggcgcaa gaaacagctg
     3601 ccggtcgggc tgcgctgccc gaggctccgg gcgctgctct gcgcgctgga gttgcctcgc
     3661 gaacgaatac gatgctctcg attcccatgt tatttttcat gggagccgct agccacttgc
     3721 cactcgcagt gacaaccgct tcgttgtggc cacttgtatt tgtgatcgcg gccgttctgg
     3781 gtgcgatcga gttcaacgca ctcaagggta aaaccggacc aatgactacc gtaaaaggtg
     3841 tgattggttg cggatttggc gtgtgtgcgg gtctctacat catcatcgag attttaacca
     3901 aaatatagtt ggtctcattc ttcgcaatcc aagaattccg tgatacgcga acgcgtatag
     3961 gggttcttgg atgcggggga gtttttacca cttcaattta gtgaaggcgg tgcttggtgt
     4021 tgtcacactg tgggcgacac tattttgcgc tatacgagcc gaggccgcca aggattaccc
     4081 aggaaacgac gcttacctgc cgagcagttg gacgtgtcgc actttactca acgaacgact
     4141 cgatgctcta actcgcaatc ctcgaattga acccgcgtta cgaatgcgtt ggcagaactc
     4201 cccggacacc tttgcgttag tcgaggccta tgcaaacaaa gcagttgttg aagccattca
     4261 gttcggagct cagcttttcg aacgtcccta tgaacgcatc gcttcggacc gaatcgtggt
     4321 ttcttggaac gctccgatgg cggcgaggtt tggcccagat ttacgccatc ttgagttgtt
     4381 tacggcggaa gagaacaatt actggttcga tagattcgaa gcgaaagcgt accgagtgcc
     4441 gcgcggtgga cgaggggcgc ttgcgcatcg aatcgggtat cgcgctttac cgacgccgga
     4501 atctaaaaaa gtttttatct tcgaagtgcg tatcaagaaa atcgaccgac atgtggccta
     4561 ccgattgttt cgagacttcg cctatagttt acatgaccaa gttttggttc cgcgccccac
     4621 gctctacaaa gatgagtttt ttggagacct agcggaaaac cgcatttgga tcgtgttgca
     4681 tgcccgaggt aaggaactac gacggcaatt cgcatactgg ggtgatctat tgagcgagtt
     4741 gcccaacatt attaatgttc agtatcactc catgacggtc gaacatctag agaacttggt
     4801 ccgagagcgc gtgatgatgg gtgacgaaaa ttcaggtatc ccaagtgggg acgcgacgaa
     4861 cgatcaccga accgattctg aggtgcgatt ttctctcttg gaactagacg aaacgcccca
     4921 ggcacgaaaa gaaagcgcag atcggatgcg aacgatcagc gaatacgaaa tccgagtgag
     4981 cctaattgag ctcgattaga tgatatattc gtatatccag tggctttgat tcaaac
//
LOCUS       DAOMXO010000080         4974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9025_length_4974_cov_5.590974, whole genome shotgun sequence.
ACCESSION   DAOMXO010000080 DAOMXO010000000
VERSION     DAOMXO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9025_length_4974_cov_5.590974"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLH57_02710"
     CDS             <1..955
                     /locus_tag="PLH57_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRK01550.1"
                     /translation="NRIVSRIVEIANKAITPYSGNYDFYERERNIRREQLAAAHQKQQ
                     DMLAKEEEFIARFAARASHAAQVQSRVKKLEKIDRIEIPAEEKSIQFTFPTPPRSGND
                     VVQISDLAKSWTLEDGREKPVFSGITGTIRRLDRIAVVGVNGAGKSTFLKVITGQTEA
                     TKGTAVVGASVKTGYFSQHSLEILDPNRTAFEEVFSRLPTPTIGYARNLLGAFLFSGD
                     EADKKVSVLSGGEKSRLILATILANPVNFLVLDEPTNHLDIKSREVLLEAIKNFEGTV
                     VLVSHDRHFLRQVANRVFEIDHGELRIYEGNYDYYQSKRPH"
     gene            1038..1113
                     /locus_tag="PLH57_02715"
     tRNA            1038..1113
                     /locus_tag="PLH57_02715"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1072..1074,aa:Glu,seq:ttc)
     gene            1278..2345
                     /locus_tag="PLH57_02720"
     CDS             1278..2345
                     /locus_tag="PLH57_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HRK01551.1"
                     /translation="MNHAELFKPIKVGSLELGNRIIMAPLTRCRAGLEHVPSEMMATY
                     YAQRASAGLIIAEATMIQEHNSAFIAEPGIYNSEQIAGWRKVTEAVHKEGGKIFLQIW
                     HGGRACHPEMNDHFETVAPSAIAIESEVVTLNGKRPHVTPRELTDAEIPTYVGYFKRA
                     ATYAKEAGFDGVEVHGANGYLIDQFLRDGSNRRSGPYGGSLQNRARFLFEVLSEVISV
                     WGSSRVGLRLSPLNSYNSMKDSDPTGLTQYLSRELNSFNLAYLHLMRSDFFGVQSAPI
                     MEVARHEFKNVLIGNMGYSPQEAAMAIAENKVDAIAFGHHYVSNPNLVQKIREGRALV
                     EPDPSTFYTPGAKGYIDYPEN"
     gene            2587..4224
                     /locus_tag="PLH57_02725"
     CDS             2587..4224
                     /locus_tag="PLH57_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01552.1"
                     /translation="MTVAGASKVLIFLLSITIFSSCSRDAFESQGGFNIYLKEEAPDL
                     YSSLAFLQSGIDNVPSTITDFSCFTVNVTGDGIQPFWDRSDNNTTSDCTDTDNFNGRG
                     QGIIARPVARGSTISLDVQSGSSRIVDVYGIYPAPIECGGSLANGSTAYAYLVGSQTT
                     DILDRTSVTIPISYAGGASKFSCIERNGPAGVAPSACSYSSTNPTYTVGTAITNNTIT
                     CSGGAPTSYSISPSINTATGLTFSTTTGTISGTPTIAAASTAYTITATNLYGSTTAVI
                     NAAALMNCTRNVATTFNAAANTLPATVTVSYSMVGGGGGGANGAAGTNGTTQSGTFTI
                     PAGQTLDVQVGGGGGGGGTGEGGGGGGGSSAIIVNGAAVAVANGGAGANLGLGGGSGG
                     GGTNAGGSGGTAGNNLGPGGAGNGGATGAGGDCGAGGATYSGGGGGAGYFGGGGGGGD
                     QNVANTGGVGGNGVNGGAAGGGGGGGGGGYGSGGGSATNAGGSNGAAGGGGATGANSW
                     AADVTLPAAAGQGGGGPSLGGNAGLVIMTYSHTSCPL"
     gene            complement(4210..4719)
                     /locus_tag="PLH57_02730"
     CDS             complement(4210..4719)
                     /locus_tag="PLH57_02730"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HRK01553.1"
                     /translation="MALPKIQADTRAWGLDHGTWSVLKHMYPKADIPVIQLSLNMKAP
                     PEYHVLLGKELSRLREKGVLILGSGNLVHNLKRIRWESDAKPYDWALEFNSWLISKLS
                     VGEYDEILNDFHRTEAGKLSIPTLDHYYPLHYILGASDSADALNIEYEEIQNGSISML
                     SFSYNSQRA"
     gene            complement(4674..>4974)
                     /locus_tag="PLH57_02735"
     CDS             complement(4674..>4974)
                     /locus_tag="PLH57_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01554.1"
                     /translation="APGNDVFRTKNVNLERSNLRSSGEAFEQKFLELKNLVMTGATLP
                     EIKHENFEKEFKNIQQSVGLIMRFNIQRRVVLSSLSLFARILRSRKFRPTHGPGV"
BASE COUNT         1291 a         1211 c         1289 g         1183 t
ORIGIN      
        1 gaaccgcatc gtctcacgaa tcgtggaaat cgctaataaa gcaatcacgc cttacagcgg
       61 caactacgac ttctatgagc gcgagcgcaa tattcgccgg gagcaattgg ccgcggctca
      121 tcaaaaacaa caagacatgc tggcaaaaga agaagaattt attgcacgat tcgcggcacg
      181 cgcttcccac gccgctcaag ttcaatcacg ggtaaaaaaa ctcgaaaaaa ttgaccgaat
      241 cgaaattcct gcagaagaaa agagtattca atttacgttc cccactccgc ctcgcagtgg
      301 aaacgacgtg gtacaaattt ctgacctcgc gaaatcttgg acactcgaag acggccgcga
      361 aaaaccagtg ttcagtggca ttacgggcac catacggaga ctcgaccgca ttgccgtcgt
      421 gggagtgaat ggcgccggaa agtctacatt tttaaaagtg atcaccggcc agacagaagc
      481 gacgaagggg actgcggtgg tgggcgcctc ggttaagacc gggtatttca gccagcattc
      541 gcttgagata ttagacccca atcgcacagc tttcgaagag gtgttttccc ggttgccgac
      601 tcccaccatt gggtatgccc gcaatctact gggagcgttt ttattcagcg gtgacgaagc
      661 tgacaaaaaa gtgtcggtgc tctcgggggg cgaaaaaagt cgtttgattc tagccactat
      721 tttggccaac cctgtaaact tcctggttct agacgaacct acgaaccatt tagatattaa
      781 atcgcgggaa gtattactgg aggcgattaa gaactttgaa ggcaccgtag tactggtaag
      841 ccatgaccgc cacttcttga ggcaagttgc caatcgggta tttgagatcg accacggtga
      901 gcttcgcatt tacgaaggca attacgacta ttatcagagc aaacgccccc actaaaccaa
      961 tctctcgaac gaatggtatt gacggtccgg ggtgccttca ctataagtgg tggttctttt
     1021 aacggcccgt atgatgcgtc cccatcgtct agaggcctag gacattgccc tttcacggca
     1081 gtaaccgggg ttcgaatccc cgtggggacg ccaaactaaa aagcctgact ggcaaagctg
     1141 gtcgggcttt ttagtttggt gtctatacaa cgaaaacacg ccgaaggcgt tttgtacctt
     1201 acacggggat gagaaagccg gagcgagcgg aagccgaatc cccaaataga ccaaatagaa
     1261 tagacgaggg agatcatatg aatcatgcag aactgtttaa acccatcaaa gtaggctcat
     1321 tggaactcgg aaatcgcatt atcatggctc cgctcactcg ttgtcgtgca ggactggagc
     1381 atgttccaag tgaaatgatg gctacttact acgcacaaag ggccagcgca ggactaatca
     1441 tcgctgaagc tacaatgatt caagagcaca actctgcttt tattgcggag ccaggtattt
     1501 acaactctga acaaattgca ggatggcgta aggtcacgga agctgttcat aaagaaggcg
     1561 gaaaaatatt tcttcagatt tggcatggcg gacgcgcttg ccatcctgag atgaatgatc
     1621 attttgaaac ggttgctcct tcagctattg cgatcgaatc agaagttgta actctcaatg
     1681 gaaaaagacc ccacgttact ccgcgtgagt taactgatgc agagatccct acctatgtag
     1741 gatattttaa acgagcagca acctatgcca aagaagcagg atttgacggt gtagaagttc
     1801 atggtgcaaa cggttacctg attgatcagt tcttacgaga tggctccaat cgacgaagtg
     1861 gtccctatgg tggaagtttg caaaatcgcg cacggttctt atttgaagtt ctgtccgaag
     1921 tgatctcggt ttggggatcg agccgagtcg ggcttagact ttctccactc aacagctaca
     1981 acagcatgaa agattctgat cccacaggtt tgactcagta tctctctcgt gagctcaatt
     2041 catttaactt agcgtatctc catttaatga gatcagattt ttttggagtt caatctgctc
     2101 ctattatgga agtggcaagg catgagttta aaaatgtgct gatcggtaat atgggctact
     2161 ctcctcaaga ggccgcaatg gcaattgctg aaaataaagt ggatgcaatt gcttttggac
     2221 atcattacgt gagtaatcca aacttggtgc aaaagattcg cgaaggtcgc gcattggtag
     2281 agcctgatcc atccaccttt tatacgccgg gagccaaagg gtacatcgat tatcctgaaa
     2341 actaacaaca caccgtcgcg cagcgattcg ggctttttta tatttgtgcc cacaatggga
     2401 tgagacgcct tgtgagcgaa gttttgggta ggggggcccc gcatagcggg gaacaaaacc
     2461 aatccccgtg gggacgccaa aaaaaagcgg ctcgttgatc gcaaggtcag cccagttcga
     2521 aataaaaaaa cataatttaa ccgacaatct tgaaatacca ataccgagag tcttacactt
     2581 gtcgccatga cggttgcagg ggcgagcaag gttttaattt ttcttttgag tattacaatt
     2641 ttctcgtctt gctcgcgcga tgcattcgaa tcacagggtg gctttaacat ttacctcaaa
     2701 gaagaagcgc ctgacctgta ctcaagtctc gcttttttgc agtcgggaat agacaacgta
     2761 ccctctacta tcactgattt ttcctgcttc accgtaaatg tgactggtga cggaattcag
     2821 ccgttctggg atcgtagcga caacaacacc acttcggact gcaccgacac cgacaatttc
     2881 aacggccgag gacagggcat cattgcgaga cccgttgcta gaggttcgac tatttcatta
     2941 gacgtacaaa gcgggtcctc acgcatcgtt gatgtgtatg gcatttaccc tgcgccaatc
     3001 gaatgcggtg gatccctcgc aaatggatct acggcttacg cctacttagt cggctcacaa
     3061 accactgaca ttttggaccg cacttcggtt acgattccaa tttcctacgc aggtggggct
     3121 tcgaaatttt catgcatcga acgcaacggt ccggcgggag tagcccccag tgcgtgcagc
     3181 tactctagca ccaatccgac atacaccgtg ggtacggcca tcacaaataa cacgatcact
     3241 tgttccggcg gagcgccgac cagttacagc attagcccca gcattaatac tgcaactgga
     3301 ctcactttca gcacgaccac cggcaccatt tcaggaactc ccaccatcgc ggccgctagc
     3361 actgcgtata cgataaccgc aacgaaccta tatggttcca caactgcggt gataaatgct
     3421 gctgcactca tgaactgcac gcgcaacgtc gcgacaacat tcaacgcagc agccaatact
     3481 ttgccggcaa ccgtgactgt ttcatactca atggtcggcg gcggcggtgg cggtgccaac
     3541 ggagcggcag gtaccaacgg aaccacccag tccgggacat ttactattcc tgcaggccaa
     3601 acactcgacg ttcaagtcgg cggcggcggt ggtggcggtg gcacaggtga aggcggcggc
     3661 ggtggtggcg gaagcagtgc gattatagtg aacggagctg ccgttgcagt ggcaaacggt
     3721 ggggccggtg ccaatctcgg tttaggcggg ggcagcggtg gtggtggcac caatgcgggc
     3781 ggttcaggcg gtacagcggg aaataattta gggcccggag gcgcaggtaa cggtggtgct
     3841 actggagctg gcggcgactg tggcgcaggt ggcgcgactt attccggcgg cggtggtggt
     3901 gcgggttact ttggtggcgg cggcggtggc ggagatcaga acgtcgccaa cactggagga
     3961 gtcggtggaa acggcgtaaa tggcggagca gccggcggtg gcggcggtgg tggcggtggt
     4021 ggctacggta gtggcggtgg atctgcgaca aacgctggcg gcagcaatgg cgccgctggt
     4081 ggtggtggtg caacaggagc aaatagctgg gctgccgatg taaccttacc cgctgcagct
     4141 ggtcagggcg gcggaggccc gagcctcggc ggcaatgcgg gactagtcat catgacctac
     4201 agtcacacgt catgcccgct gtgaattata gctgaaactt agcatcgata tagagccgtt
     4261 ttgaatttct tcatattcta tattcaatgc atcagcagag tcagatgcac ccaatatata
     4321 atgtaacgga tagtagtggt ccaaagttgg aatactcagc tttcctgctt cagtccgatg
     4381 aaagtcattt aagatctcgt cgtattctcc cacagacagt ttactaatta gccacgaatt
     4441 aaattctaac gcccagtcgt aaggcttggc atccgactcc cagcgaattc ttttcaaatt
     4501 atgtacaaga ttgccgctac ccaaaatgag cactcctttt tcgcgcaggc gcgaaagctc
     4561 tttacccaga agcacgtgat attcaggagg ggctttcatg tttaaactga gttgaatcac
     4621 cggaatatcg gccttcggat acatgtgttt caacacggac caagttccat gatctaaacc
     4681 ccaggcccgt gtgtcggcct gaattttcgg gagcgcaata ttctcgcgaa taaactgagc
     4741 gaactgagga ctacccggcg ctggatattg aatctcataa tcaagccaac cgattgttgg
     4801 atatttttaa attccttttc aaaattttca tgcttaattt ctggaagtgt tgccccagtc
     4861 ataactaggt tttttaattc taggaatttt tgctcaaagg cctccccgct agagcgaaga
     4921 ttgctgcgct ctaaatttac attcttggtt ctaaaaacat cattaccagg tgca
//
LOCUS       DAOMXO010000081         4973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9030_length_4973_cov_4.352582, whole genome shotgun sequence.
ACCESSION   DAOMXO010000081 DAOMXO010000000
VERSION     DAOMXO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9030_length_4973_cov_4.352582"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1524)
                     /locus_tag="PLH57_02740"
     CDS             complement(94..1524)
                     /locus_tag="PLH57_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRK01555.1"
                     /translation="MELTRRSIIEPFKIKMVEPLHLHSLEAREKFLKDAGYNLFLVPS
                     QGVTFDFLTDSGTTAMSANQWGAMMVADESYAGSRSFQKFEAAVGEVTGYNYVIPTHQ
                     GRAAERLLFGLLVKPGDQVPSNNHFDTTRANIEQIGAQAVDLVIPEGRDPKSRHPFKG
                     NIDLELLEEFCKKHRARIPIGMLTITNNTGGGQPVSFENIRGAAAIYRKYNIPFILDA
                     CRFAENAAFIRNRERGMSERSIRSIAREVFDTADGCMMSAKKDGMVNIGGFIALKKQD
                     WVTDLRNNLILTEGFPTYGGLAARDLEALAVGLFEALNEDYLEYRLAVAKYMGDGLTD
                     LGIPIVQPPGGHAVYIDAKAFLPHIPPSQFPGQALVVELYRREGVRSCEIGSVMFGRT
                     ESEKFVPAAMELVRLAFPRRVYTQSHFDYLLEGFELLAKEKQKCRGLQMTYSPPFLRH
                     FTAQFKPANRRAVERKWVACAPEKSN"
     gene            1619..2536
                     /locus_tag="PLH57_02745"
     CDS             1619..2536
                     /locus_tag="PLH57_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRK01556.1"
                     /translation="MAGNFRWIGKSLDGESVTLKRFRGTFNCAEYATPAWLEQNREVV
                     RTAAVLDLETTGLNARTDQIIEIGVRKFKFNRATGEMLEAGASYNEFHDPGVPISPEL
                     TRLTGITNEMVAGKKIDWAAVDHFISDCSIILAHNAAFDRPFTDARSSISSEIPWGCS
                     FKQVAWDEKGFPSRKLEVLSIYHGFFSDSHRAIHDADATLHLLTFSDPTTCKPYLGEL
                     LNNARKPMSRVFALGSPFETKDLLRSRGYQWFTPGPGTTFPEKCWVRTLPKDHLAEEI
                     AWLESAVYKGTFRGAVNDIPITDNFKSIG"
     gene            2496..3293
                     /locus_tag="PLH57_02750"
     CDS             2496..3293
                     /locus_tag="PLH57_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRK01557.1"
                     /translation="MTSQLPTISSPSANRGELLGLFYSPYTERARWALDHHEVPYKYS
                     EYLMMLGDPLLHWKARSLKSNVTVPFFIHGTRRLGDSYAIAQYADQIGTGSKLFSPND
                     LAAIDQWNRASEATLGAARAVVVHRLRENSEARLDHLPKLLPEPFNGALRSAAGPIAN
                     LAIRYLQKSFQIKDSTAEHLTELRKRFLALRQALGGRPYLLEKFSYADITSSVCVQAL
                     EPVEEPYLSLGSAAREAWRIGELAREFSDLIEWRNSLYRKHRTRSAC"
     gene            3374..4348
                     /locus_tag="PLH57_02755"
     CDS             3374..4348
                     /locus_tag="PLH57_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01558.1"
                     /translation="MSSFYSFIKKIAIFLVVGHLIGAAIHAASKRPPQLRKLASSNEC
                     FLQSADSSDAPPRALDCSMTAELTQQYQMAVQPIIDSYSQHGEVDKETLRSAESVLSC
                     LSQCRPCSEMGFTIAKTLDYGWALQTSAEVTSQCTINLPDLKKKHHKVVSGYGSEDTR
                     SAASVNPIRGRLSYSPSGNWKCHLTSGESKKVSREGSKRRLYFYSRGVFVQDDTVSPS
                     KKSATAMRARWVHFTRYTEAAPIELKAIDKNTLRFIWPKQAGLEPFLEINHEGYPVSS
                     NFVDSNTWLTSQCQTDYRRGKYYPRLSVLDDSITEICEDGDLPPCNSR"
     gene            complement(4352..>4973)
                     /locus_tag="PLH57_02760"
     CDS             complement(4352..>4973)
                     /locus_tag="PLH57_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01559.1"
                     /translation="AIIEKQVRKNIDSKYFIDSDARLKEQGVPYLRGETVDVRFYPAA
                     KSIGVSACAMDINDLLKAYLKGDTKPLETSFWWKGFSQHQEFRPLCIRVDLPVLDFDP
                     TKVDLKSEFKKRLESAISEVSAWTPPPAYYDSLEKYLDHVLPKVCRPKSVESTEVSIT
                     PEPVETQAAGETPVDEATPNQGEQPTQDSSETGETSDNSSGDDVDL"
BASE COUNT         1266 a         1307 c         1138 g         1262 t
ORIGIN      
        1 aaaataaccc cgccagtaca acccagcggc cgttccgaaa aatcatgccc ggctttgtag
       61 catagagtgt ctaacaaaag ctatgcggtc cgactaattc gatttttctg gagcgcaggc
      121 cacccacttt cgctccaccg cacgacgatt tgccggtttg aactgtgcgg taaagtgccg
      181 caaaaacggg gggctatagg tcatttgcag accccgacac ttttgttttt ccttggcgag
      241 tagttcaaac ccttcaagca aataatcaaa atggctctgg gtgtacaccc gtctcggaaa
      301 ggctaaccgg accaattcca tggcggcagg tacgaatttt tcactttcgg tgcgcccaaa
      361 cattacggaa ccgatttcac acgaacgcac cccttcgcgc cggtagagct caactaccaa
      421 agcctgcccc ggaaattggc ttggcggaat gtgcggcaga aaagcttttg catcaatata
      481 cactgcgtgg ccgcccggtg gttgaacaat tggaattcca agatctgtca aaccatcgcc
      541 catgtattta gccaccgcca aacgatattc taaataatct tcgttgagcg cttcaaacaa
      601 accaaccgca agtgcttcca aatcgcgtgc cgctagacca ccataagtag gaaacccctc
      661 agttaaaatc aggttatttc gcaggtcagt cacccaatct tgtttcttaa gcgcgataaa
      721 accgccaata ttcaccatgc catctttctt ggcgctcatc atacaaccat ctgcggtgtc
      781 gaacacctca cgcgcaatcg atcgaataga cctctccgac attccccgtt cacgatttcg
      841 aatgaaagct gcattttcag cgaaacggca ggcgtctaaa ataaagggga tattatattt
      901 tcgataaatt gcagcagcac cccgaatgtt ttcgaacgaa acgggttgtc caccaccggt
      961 attattggtg attgtcagca ttccaattgg gattctagcg cgatgctttt tacaaaattc
     1021 ttcgagcaat tctaagtcga tattaccttt aaacggatgg cggcttttgg gatcccgacc
     1081 ttctggaatc accagatcga cggcttgggc gccgatctgt tcgatattcg ccctggtcgt
     1141 atcgaagtga ttgttcgacg gtacttggtc gccaggttta actagcaaac caaacaagag
     1201 gcgctccgcg gctcgccctt gatgggtggg aatcacgtaa ttataaccgg tcacttcgcc
     1261 cactgctgct tcgaactttt gaaaactccg cgaaccagcg tatgattcgt cggccaccat
     1321 catagccccc cactgattgg cgctcatggc agtggttccc gagtctgtta gaaaatcaaa
     1381 cgtgactccc tggctcggca ctagaaataa attataacca gcgtctttta aaaatttttc
     1441 gcgcgcctct agcgaatgca aatggagcgg ctccaccatt ttgattttga acggttcaat
     1501 aatactgcga cgagttaatt ccatttctcc cccacacagc tcatatgaaa atgcttgttc
     1561 ttgtcataca ccaacccgtc tggcattgtc gtgacgcaga gaggcaggtg tcgtaacagt
     1621 ggcaggcaac tttaggtgga tcggtaaatc gctcgacggt gaatcagtta ctctgaagcg
     1681 ttttcgaggt accttcaatt gcgcggaata cgccacccct gcttggttgg agcaaaaccg
     1741 tgaagtcgtg cgcacggcag ccgtacttga tttagaaact accgggctaa acgcgcgaac
     1801 cgatcagatt attgaaatcg gtgttcgaaa atttaagttc aatcgcgcaa ctggcgaaat
     1861 gctcgaagcg ggcgcaagct acaatgaatt tcacgatcca ggagttccca tatcccccga
     1921 acttacgcga cttactggca ttaccaatga aatggttgcg ggaaaaaaaa tcgattgggc
     1981 cgcagtcgat cactttattt cggattgttc aattattctt gcgcacaacg cagcctttga
     2041 tcgtccgttt accgatgcac gttcttcaat cagctccgaa attccctggg gctgctcttt
     2101 caaacaagtg gcctgggacg aaaaaggatt tccaagccga aagctggaag tgctcagcat
     2161 ttaccacggt tttttttcgg actcccaccg agctattcat gacgccgacg ctaccttgca
     2221 tttgctcacg ttttcagacc cgacgacctg caaaccatat ctcggcgaac ttttaaataa
     2281 tgctcgtaaa cccatgagtc gagtatttgc tcttggttcc ccctttgaaa caaaggatct
     2341 attgcgatcg cgcggttatc agtggtttac accgggcccc ggtaccacgt ttcccgaaaa
     2401 atgttgggtc cgaacactgc ctaaggatca tttggccgaa gagatcgctt ggctcgagtc
     2461 tgccgtttac aaaggaactt tcagaggagc tgtaaatgac atcccaatta ccgacaattt
     2521 caagtccatc ggctaaccgc ggcgagttgc tcggactctt ttattcgccc tacaccgaac
     2581 gcgcccgttg ggcgctcgat caccatgagg tgccttataa atattccgag tatttgatga
     2641 tgctgggcga tccacttttg cactggaaag cacgatcgct taagtcaaac gtaacggttc
     2701 cattctttat tcatggcacg cggcgtctcg gtgattccta tgcgatcgca cagtacgcgg
     2761 atcagattgg aactggctca aaattgttct cgccaaacga tcttgctgcc atcgaccaat
     2821 ggaatcgcgc ctctgaagcg acactaggtg ccgcaagagc agtggttgtt catcgcctgc
     2881 gcgagaactc tgaggcgaga cttgatcatt tgcccaaatt gctgccagaa ccctttaacg
     2941 gagcgctgcg atctgcagct ggaccgattg ccaatcttgc tattcgttat ttgcaaaaaa
     3001 gttttcaaat taaagattcg acagcggaac atctcacaga actgcggaaa cgttttctcg
     3061 ctctacgcca agcactcggc ggtaggcctt acctactaga aaaattttcc tacgccgata
     3121 tcacttcaag cgtttgcgta caggccctgg aaccggtgga agaaccctac ctgtcgctag
     3181 ggtccgctgc acgcgaagcg tggcgcattg gggaactcgc gcgagagttt tctgatttga
     3241 tcgaatggcg aaactcgttg tatcgcaaac atcgcacgcg ctcggcgtgt taagtaggtt
     3301 cactcgaaaa cggcaggttt ttcttcattc ttttacacaa ctttcgtaaa aaaatgaggt
     3361 attcttaata acgatgagtt cgttttattc tttcataaaa aaaatcgcta ttttcctggt
     3421 tgttggccac ctaatcggag cggcaataca cgctgcctcc aagcgccccc cacagctgcg
     3481 aaaactcgca agctccaatg agtgttttct gcaatcagcc gattcttccg atgccccgcc
     3541 acgagctctc gactgcagca tgacggccga attgacgcag caataccaaa tggccgttca
     3601 accaattatt gattcttact ctcagcacgg cgaggtcgat aaagaaactc tccgaagtgc
     3661 ggaatctgta ctatcatgcc tttctcaatg cagaccttgc tctgaaatgg gatttacgat
     3721 cgctaagacg ctcgactacg gatgggcgct ccagacctca gcggaggtaa cttctcaatg
     3781 caccatcaat cttccagatc taaaaaagaa gcaccacaaa gtcgtatcag gatatgggtc
     3841 cgaagatact cggtccgccg catctgtgaa cccaatcagg ggccgtcttt cctattcgcc
     3901 aagtgggaac tggaaatgcc acctgacgag cggtgaatca aaaaaggttt cccgtgaggg
     3961 ttccaaacgg cgactctatt tttattcacg cggagttttc gtgcaggacg acactgtaag
     4021 tccgtcaaag aagtccgcca ctgcgatgcg agcgcgctgg gtgcatttca cgcgttatac
     4081 cgaagcggcg cccattgaac ttaaagcgat cgataaaaac accttgcggt ttatctggcc
     4141 caaacaagca ggtttagagc cgttcttaga aataaatcac gaagggtacc ctgtgagttc
     4201 caacttcgtc gactccaaca cgtggctgac gagtcaatgt caaacagact accgcagggg
     4261 caagtactac ccgcgactca gtgtccttga cgattcgatc actgaaatat gtgaagatgg
     4321 cgatttgcca ccgtgtaata gccgctagca attataggtc gacgtcatca ccggaactat
     4381 tgtcagaagt ttcaccggtt tcactactgt cctgcgtcgg ctgttcgcct tggttcggag
     4441 tagcctcatc gactggtgtt tccccggcgg cctgagtttc cacaggctcg ggcgtaatgg
     4501 atacttccgt actttcgaca gactttggtc tacagacctt cggcaaaaca tgatccaaat
     4561 atttttcgag tgagtcgtaa taagctggtg gcggcgtcca ggcggaaacc tcgctgattg
     4621 cgctttccaa gcgtttttta aactcgctct ttaaatcaac cttagtcgga tcgaagtcta
     4681 atacggggag gtcaactctg atacacaacg gcctgaattc ttgatgctgt gagaagcctt
     4741 tccaccagaa cgaagtttcg aggggcttgg tgtcaccttt caaataggct ttcaacaaat
     4801 cattgatatc catagcgcac gcggacaccc cgatagactt ggcagccgga taaaaacgaa
     4861 catcgaccgt ttcgccacga agatatggca cgccctgctc ttttaatcgt gcatcgctat
     4921 cgataaagta tttcgaatca atattttttc taacttgttt ttcaatgatc gca
//
LOCUS       DAOMXO010000082         4956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9085_length_4956_cov_5.345032, whole genome shotgun sequence.
ACCESSION   DAOMXO010000082 DAOMXO010000000
VERSION     DAOMXO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9085_length_4956_cov_5.345032"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLH57_02765"
     CDS             <1..200
                     /locus_tag="PLH57_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01560.1"
                     /translation="TLSDRYHIFVYERDASKLLSSVPAFDSFICAFVALQYSLGRVVD
                     FKHELNQKEILKTGWIELPNF"
     gene            210..1133
                     /locus_tag="PLH57_02770"
     CDS             210..1133
                     /locus_tag="PLH57_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK01561.1"
                     /translation="MASFVIKRILLAIPTLIVLMTLTFAIMKAVPGGPFDSEKALPDD
                     VRANLEAKYHLDKPLVEQYFLYLGNLVRGDLGPSYRYLGTRSVGEVIAETLPISMILG
                     FWALLFAIGIGIPLGVASAYFRHSWIDISAMFMAIAGVSLPNYLVASALILIFSMYFQ
                     WLPPALWDDWTSAVLPTITLGLRPMALIARMTRASMLDVLNADYVRMAYAKGAGTLRV
                     LFVHALKNALVPILTILGPITAAIITGSFVVEVIFAIPGMGRHFVSAVVDRDYTLILG
                     VTLTYGIIVIIANLLVDMLYAWADPRIRVKA"
     gene            1130..1966
                     /locus_tag="PLH57_02775"
     CDS             1130..1966
                     /locus_tag="PLH57_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK01562.1"
                     /translation="MKAEFAKKFLRNRAGVAGLLFIIFVCTCATFAPWIATHSYEEQN
                     IDQRLSSPNAEYWMGSDSLGRDLYSRIVYGARTSMAVGIITALVAVFIGTFYGALSGY
                     FGGWVDAFLMRVVDIFYIFPSLLLAILIMVVWGQGLVGIIAALSIVGWVAQARLVRGQ
                     VLQVKEMLHVENARALGVPPIRLLWRHVLPLIWGPVIVQITFQIPANIMAESFLSFIG
                     LGIQPPLSSWGTLANEGWRGMRSYPHLIIFPGAVLFLTMLAFQFFGDGLRDWLDPYQT
                     NK"
     gene            complement(1956..3509)
                     /locus_tag="PLH57_02780"
     CDS             complement(1956..3509)
                     /locus_tag="PLH57_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRK01563.1"
                     /translation="MRTKTSILFASIFFLLSIFVHAENTTKTFRMRLVADPQTFDWNI
                     ATTPMETYLLMNIMEGLVEFDGGMKLRAALAKSWTVSEDQRTYTFKIRPGVKWSDGKF
                     LTAHHFADSWERLLNPLTAAPYAYMLFDIQGAEEYNARKHTDFSKVGVRAIDAATLEV
                     RLKRPVGYFLQMLTFWVTFPIRKDLVAQHGTAWTKPGKIVVLGPFVPTQYQQGNLISL
                     TRNDLYYRGRPKLDEVHLKIVNEDSTALNLFKTGELDCVLPINFLELGDLIKSPSYKN
                     VPYFRTSFIGFNTQKYPTNLPNVRQAIAMAIDKKNVGKVMHQSKIPANSLVPPSMFPA
                     GQNSGLEFDPNKARKVFKESGLEPSTLPPLELLTYSSDENALLGQYIQDQLKKHLGLK
                     VTLNMPEYKTFRTQLDLQTAAMHYRPWGADYSDPDTFFGVFTSWSGNNRTGWKNERYD
                     EIVKIAASIQNGTERTKLYKEALEILLRKDAAIVPLHYDTLAFLLNPKIKGFVLNPLN
                     YVYFRDITY"
     gene            complement(3543..>4956)
                     /locus_tag="PLH57_02785"
     CDS             complement(3543..>4956)
                     /locus_tag="PLH57_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRK01564.1"
                     /translation="EIKLAAETALGRPVEDAVITVPAYFNDSQRQATRAAGRLAGLNV
                     LRILNEPTAASLAYGLHEKRNGLVAVYDLGGGTFDVSILKLRDGLFEVLSTAGDTQLG
                     GDDIDIAIARHLGLEADSGKALIIAENLKIALTTQKSATFEGYALSREQLETIVVPIL
                     EKTRTAVEQSLIDAKIDRTELTDVVLVGGPTRMPVVQQFAAQLFGRQPNCNLNPDEVV
                     ALGAAVQADILAGNNRDFLLLDVVPLSLGIETMGGTMNKLIPRNTRIPAMAKEQFTTY
                     AEGQTKVAINVYQGERDLVTFNRKLGQFVLAGIPPLPAGLARVEVTFLVDADGILSVS
                     AKELYSEVEQSIEVRPSYGLTDEQVEQMLAAYLDNKDEDLSVAKLVEARNDARTVLTA
                     TEKALPKVADLLDKNERINIETLTANLRGVIEKEDLTAIKNAHKALSDGTRHLAELLI
                     QATLDKNFKNKNVDGFLKKG"
BASE COUNT         1202 a         1085 c         1193 g         1476 t
ORIGIN      
        1 aaactctttc ggatcgatac catatatttg tttacgaacg cgatgcatct aaattgcttt
       61 caagtgtacc ggcgttcgac tcgtttatct gtgcttttgt tgctcttcaa tatagcctag
      121 gaagagtggt cgactttaaa cacgagctca accaaaaaga aattctgaag acgggttgga
      181 tcgaattacc gaatttttga tgaggcgcaa tggcttcgtt tgtaatcaaa cgcattctct
      241 tagcaatacc tacgcttatt gttttgatga ctctgacgtt tgccatcatg aaggcagtcc
      301 ctggtgggcc gtttgattcc gaaaaagcgt tacccgacga tgttcgggct aatctagaag
      361 cgaagtacca tttagataaa ccactagttg aacagtattt tttgtatcta ggaaatctag
      421 ttcgtggcga cctgggaccg agttataggt acttgggcac taggagcgtc ggcgaagtaa
      481 ttgccgaaac actgcccatc tcaatgatct tggggttctg ggcactgtta tttgctatcg
      541 gtattggtat tccgctagga gttgcatcag cctattttcg acatagctgg atcgatattt
      601 cagccatgtt catggcaata gcaggtgtct cattgccgaa ctacttggta gcgagtgcgc
      661 taatcttaat tttcagtatg tatttccaat ggctaccacc ggcgctgtgg gacgactgga
      721 cttctgcagt attgccgacg atcacgctag ggcttcgacc aatggctttg atagcgcgaa
      781 tgacgcgagc gtctatgctc gacgtattaa atgccgatta tgtgcgaatg gcatacgcaa
      841 aaggcgcggg caccttgcgg gtattgtttg tgcacgcctt aaaaaacgca ttggttccga
      901 ttctgactat attgggcccg ataacagctg cgattatcac tggttcattt gtggttgaag
      961 tcattttcgc catacccggc atgggtcgcc atttcgtaag tgctgtggtg gatcgagatt
     1021 atactttaat tttgggagtg acgctgactt acggaattat tgtaataata gctaatctgc
     1081 tcgtagatat gctttacgct tgggcagacc cgagaattcg ggtgaaggca tgaaggctga
     1141 attcgctaaa aaatttctaa gaaatcgtgc gggtgttgca ggtctattgt ttattatttt
     1201 cgtgtgcacc tgcgctactt ttgctccttg gattgccact cattcgtacg aagaacaaaa
     1261 tattgaccaa cgtttgagtt caccgaatgc cgaatattgg atgggtagtg attcgctcgg
     1321 gcgtgacctc tacagtcgca tcgtctatgg tgcgcgcacg agtatggccg tcggtatcat
     1381 aaccgcgtta gtggcggtgt ttattggtac cttttatggc gcactttccg gttatttcgg
     1441 aggttgggtg gacgcgtttc tcatgcgcgt cgtcgacata ttttatattt ttccatcctt
     1501 gttattagcg attttaatca tggtggtgtg gggccaagga ttagtaggaa tcattgccgc
     1561 actcagcatc gtaggctggg tcgcccaagc gcggttggtt cgagggcagg ttctacaggt
     1621 aaaggaaatg cttcacgtcg aaaatgctcg cgcactcggt gttcccccaa ttagattgct
     1681 gtggcgacac gtattacctc ttatttgggg tccggtgatt gttcaaatca ccttccagat
     1741 tcctgcgaat ataatggccg aaagtttttt aagtttcatc ggacttggaa ttcaaccgcc
     1801 actttctagc tggggcacat tagctaacga aggttggcga ggaatgcggt cttatccaca
     1861 cttaatcatt tttccaggtg cggttttatt tcttacgatg ctcgcatttc aattctttgg
     1921 agacggtttg cgagactggc ttgatcctta tcaaactaat aagtgatatc tctaaaatac
     1981 acgtaattta gcggattcag gacaaaccct ttgatcttcg gatttagtaa aaatgccaat
     2041 gtgtcgtaat gaagcggtac gatcgctgca tctttacgca aaagaatttc aagagcttct
     2101 ttgtacaact tcgtacgttc ggtaccattt tgaatgcttg cggcgatttt cacgatttca
     2161 tcataacgtt cgtttttcca gccggtgcgg ttgtttccgg accacgaagt aaaaacgcca
     2221 aaaaaagtat cggggtctga gtagtcggct ccccaggggc ggtagtgcat tgctgcggtt
     2281 tgcaaatcga gttgcgttcg aaaggtttta tattccggca tattcaacgt tacttttaga
     2341 cccagatgct tttttaattg gtcctgaata tattgcccaa gtagggcgtt ttcgtcgctg
     2401 gagtaggtta gtagttctag tggcggaaga gtactaggtt ccaagccaga ttctttaaag
     2461 acctttcgtg ctttatttgg gtcaaactct aacccggaat tctggccagc gggaaacatc
     2521 gaaggaggaa ccaaggaatt tgctggaatt ttcgactggt gcataacctt gcctacgttc
     2581 tttttatcta ttgccatggc tattgcctgt cgaacgtttg gcaaattagt aggatacttc
     2641 tgggtattaa accctataaa gcttgtgcga aaatagggaa cgtttttgta ggatggtgat
     2701 tttattaaat cgccaagttc cagaaaatta atcggaagca cgcagtctag ttcgccagtt
     2761 ttaaaaaggt tcaatgcagt ggaatcttcg ttaacaattt taagatgcac ttcgtctagt
     2821 ttgggacgac cgcgatagta cagatcgttg cgtgttaacg aaattaaatt accctgttgg
     2881 tattgagtag gaacgaaagg tcccaatacc actatttttc caggcttagt ccaagcggtg
     2941 ccatgttggg cgacaaggtc ttttcgaatg ggaaatgtga cccaaaatgt aagcatttgt
     3001 agaaaataac cgacgggcct ttttaatcgt acttctagag tagcggcgtc aatagcccgc
     3061 actccaactt ttgaaaaatc agtgtgttta cgggcattgt attcttctgc cccttgtata
     3121 tcgaaaagca tataggcgta gggcgccgcg gtaaggggat tcaataaacg ctcccaggag
     3181 tctgcgaaat ggtgtgctgt aagaaatttc ccatcggacc attttacacc gggtcgaatt
     3241 ttaaaagtgt aagtgcgttg atcttccgaa acggtccagg acttcgctag ggcagccctt
     3301 aatttcatac ccccatcgaa ctctaccaac ccttccatga tattcatcaa gaggtaagtt
     3361 tccatgggcg tagttgcaat attccaatcg aaagtttgag ggtcggctac taaacgcatg
     3421 cgaaaagtct tggtggtgtt ttcagcatga acgaaaattg aaagcagaaa aaatatactt
     3481 gcgaacagta tactggtttt ggttctcacg ggtccccctt cgatcgtctt aagaatagca
     3541 gttcagcctt tttttaagaa gccgtctaca ttcttgttct tgaaattttt atctaaggtg
     3601 gcctgaatca ggagctctgc taggtgacgg gtgccgtcgc tcaaagcctt gtgagcattt
     3661 ttaattgctg tcaggtcttc tttctcgata acaccacgca gattagcggt taaagtttcg
     3721 atgtttattc tttcattttt gtccaaaagg tcagcgactt ttggaagagc tttttcggta
     3781 gcagttaaga cggtcctcgc gtcattacga gcttctacca atttggcgac agaaaggtct
     3841 tcgtctttgt tatccaagta ggctgccagc atctgttcga cctgttcgtc tgtaagcccg
     3901 taacttggcc gcacttcaat gctttgctct acttcgctat aaagttcctt tgcagaaaca
     3961 ctcaatattc cgtcggcatc taccagaaag gttacctcga cgcgagcaag tccagcaggt
     4021 aagggcggta ttcccgctaa aacgaattgc cccaattttc gattgaaggt tacgaggtct
     4081 cgttcacctt gataaacatt gatggcaact ttggtttggc cttctgcgta agtggtgaac
     4141 tgctccttgg ccatggcggg aatgcgcgtg tttcgcggaa taagtttatt cattgttccg
     4201 cccatggttt ctattccgag gctaagtgga accacatcga gcaacaaaaa gtctcgatta
     4261 tttccagcga gaatatcggc ctgtaccgcg gctcccaacg ctaccacttc gtctggattt
     4321 agattgcaat tgggctgtct cccaaaaagc tgggccgcaa attgctgaac tacaggcatg
     4381 cgagttgggc cacccactaa caccacgtcc gttagttccg ttcggtcaat tttcgcgtct
     4441 atgagcgatt gctcgaccgc ggtccgtgtt ttttctagaa taggtacaac gatagtttcg
     4501 agttgttccc gggaaagagc ataaccttcg aaagtcgcac tcttctgtgt ggtcaatgcg
     4561 atcttaagat tttcggcgat aattagagct ttaccggagt ctgcttccag cccgaggtgc
     4621 cgcgcaatcg caatatcaat gtcgtcgccg cccagttgag tatctccggc ggtagacaaa
     4681 acttcgaaca ggccgtctcg caatttcaaa atcgatacgt caaaagtgcc gcccccaaga
     4741 tcatagactg ccacgagacc atttctcttt tcgtggagcc cgtaggcgag tgaagcggca
     4801 gtcggttcat ttaagatacg tagaacattt aatcccgcca acctgcccgc cgcgcgagta
     4861 gcttgccgct gactgtcgtt aaaataagcg ggaacagtga ttacagcatc ttctaccggg
     4921 cgacctagtg cggtttcagc ggctaatttt atttct
//
LOCUS       DAOMXO010000083         4914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9202_length_4914_cov_6.838238, whole genome shotgun sequence.
ACCESSION   DAOMXO010000083 DAOMXO010000000
VERSION     DAOMXO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9202_length_4914_cov_6.838238"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4774)
                     /locus_tag="PLH57_02790"
     CDS             complement(<1..4774)
                     /locus_tag="PLH57_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRK01565.1"
                     /translation="MFVSRKSGYVFALIASIALFNTGCSFTEVADEIQNNSQEVVRLV
                     EPFFGRNSGNLVADPGFETGVSGFEADQETTAVIWLPDAAAPDRLQLAGAHSIQIRTQ
                     IWGDSIWWAKQVEEAHLQHASRLEISAHFRSDLASASKLLLCGYINYADGSATSRCEE
                     IQANLGDKGKVTTMLELDGTKTLERVRIRLTQEGGAPVQFTVDSAQVVLYGISEVPVP
                     TPVPTPEPTPAPTPDPTPDPTPVPTPVPTPDPTPTPAPTPAPTPAPTPTPTPAPTPAP
                     TPVPTPAPTPVPTPTPTPEPVTSLYVDPTGDDANLCTQSAPCRQVRRALVFAAPGSTI
                     YVANGDYLGFSISNLTGTAEQPIVVRGTGNAVNIVPSTDRGAIYDRDNIIVEWSEHLV
                     FENLRSSNAVRSGMRVSTSRFIQVRNSTFANNGKWGFFTDFADDVVFENNTVSGTVEQ
                     HGVYFSNSNQRPTARGNTIFANGQSAIQINADRFFAPPTGWTPEVFGITRGATIENNT
                     LYGNGARGGAAINLDGVQNSVVRNNVLYGNSAGGITNFQIDGAEGPRDMLVVHNTILH
                     QNSTRAAIQFSNSTGLNNHTNNLVYHPNGVGLELVSDSDRLFIRSNNNILDNVYASNR
                     TITLAQWQGESAQDGASIATNGLSLNSIFVNPTIYNYRLVENSPAVDNGMTLSSGVAD
                     SDFDDVQRPRGNAYDIGAFESEFTKPAPTPVPTPSPTPDPTPTPTPSPAPTPGPTPDP
                     TPTPDPTPAPTPAPSATPVPSATPAPTATPAPTATPSPTPAPSDVIQVTVNLTPHAET
                     IGQSTLVSFGVPLPPGVLYDTSQIVVRDETGIEIPVFIKSLGNWTEMPPESMLCNGLD
                     PARAPGVRSVFVQFTRSFTSTATIPYVVELNANPSTARLAEANVRTGYRLVTEGSFQS
                     ADNVQEPLVYATLPSRWLACSLIAPNEQPLSSDYLGTETVNQLNNWFTSFTNPATTPY
                     RTGYEPWLYDRSQTFFNNYIRSGSITHLREAIRNTQFFFDRFSNAGSFSMRAGDPKYS
                     YNENQYSHYLLTGDSQITSKIVSSVAAHTQINRNSFFTERNLAMGLHAQVTRYDFAAS
                     TGLNPTNVATYRSAIATTIGEVEWMINNPNFRYSYGPNALRTGCINYYVEDDSTTLGF
                     SPWMSSLLAQALARAYVSTGDTRIPTIMTDLGHCLVDYGSVTSGAYLLPLYTASAVSA
                     TPQWPNGDTDGDSQHALDVGYAVALAAYLTPDSAAKTKFTNAANQYLTAHRGTAALGG
                     WSSANTRRYSWFYKAGPAMDWLITGKIDSPVITSGGPSPSPTPVPSATPAPTATPAPT
                     PTPTATPAPTPGPTPAPTPVPTATPLPTATPAPTPTPAPGTSGNLVADPGFENGIDGI
                     GDFGTGVTGIHSQTNPISGTASLVISIAETRYGAAAWWTHRANGSVTAESYDISARLR
                     SNVQSTSNFAFCAFIYYLNSGSPAQQCVEVSGALGDKGTATVHLVLDPTRAIDLVRVR
                     FVQNGSNRTEFAVDELSAVLNGMSGTLPSPSPTPAPTPVPTPAPSLPPGATPTPTPVP
                     SATPVPTPNPGGSQGMNIPLSHPRLWFNAERLDRARTWI"
BASE COUNT         1148 a         1075 c         1439 g         1252 t
ORIGIN      
        1 gaatccaagt acgagcgcga tccaagcgtt cggcgttgaa ccacaatcta ggatgcgaaa
       61 gtggaatgtt catgccttga gaaccaccag gatttggtgt tggaactggt gtcgcacttg
      121 gtacaggagt tggagtcgga gtggcgcctg gcggtaaact cggtgctggc gtcggaactg
      181 gagttggcgc tggcgttgga gaaggtgatg gcaaggttcc actcatgcca ttcaacactg
      241 cgcttagttc gtcgactgcg aattcagtgc gattactgcc attttggaca aagcgaactc
      301 gaactaaatc aatagcacgc gtaggatcaa gcacaaggtg cacggtcgcg gttcctttgt
      361 ctccgagtgc accagaaact tcgacacatt gctgagcggg tgaaccagag ttcaaataat
      421 atatgaacgc gcagaacgcg aagttcgaag tgctctgaac gttcgatcga aggcgagcgg
      481 agatatcgta agactcagca gtcactgagc cgtttgcacg atgagtccac caagcggcgg
      541 ctccgtatcg agtttccgca attgaaatca ctaagctggc ggtgccgctg attggatttg
      601 tttgggaatg aataccagtt actccggtac caaaatcacc gataccatcg attccgtttt
      661 cgaacccagg gtcggctact aagttgccag aagtgccagg ggccggagtg ggcgtaggtg
      721 ccggagtagc agttggcaat ggagtagctg tcggtactgg agtcggtgcc ggtgttggac
      781 ctggtgttgg agccggcgtt gccgtagggg ttggtgtcgg agccggtgtg gccgtaggag
      841 ccggagttgc gctcggcact ggtgttggtg aaggacttgg tccgccacta gtaatgactg
      901 gagaatctat tttaccagta atcaaccaat ccattgcagg gccagctttg taaaaccagc
      961 tatagcgacg ggtgtttgca gaactccagc caccgagagc cgcagttccc cgatgtgcag
     1021 ttaggtattg atttgctgca ttggtgaatt tggtttttgc tgctgaatcg ggcgtgagat
     1081 atgcggcaag tgctacggcg taaccaacat cgagcgcgtg ttggctgtca ccatcggtgt
     1141 caccgtttgg ccattgtgga gtagcgctta ctgcagatgc agtatagagc ggcaacagat
     1201 atgcgccact ggttacacta ccataatcca ccaaacagtg accgagatca gtcatgattg
     1261 taggaatacg ggtatcgccc gtcgacacat aagcgcgggc caatgcctgc gccaaaagac
     1321 tgctcatcca aggtgaaaat ccgagagtag tagaatcatc ttcgacgtag taattaatgc
     1381 aaccagtgcg aagggcatta ggtccgtaac tgtatctaaa attcggattg ttaatcatcc
     1441 actcgacttc acctatagtg gttgctatcg ccgaacggta agtagctacg ttagttgggt
     1501 ttagaccggt tgaggccgcg aagtcgtaac gcgtaacttg cgcgtgtaag cccattgcca
     1561 gatttcgttc cgtaaaaaag ctgtttcgat taatttgggt gtgagcggcg accgaagaca
     1621 caattttgct agtgatttgt gagtcaccgg ttaacaagta atgggagtat tgattttcgt
     1681 tgtaggagta tttcggatca cccgctcgca tcgaaaacga accagcgttg ctaaatcgat
     1741 caaagaagaa ttgagtgtta cggatcgctt cacgcagatg cgtgatgctg ccagatcgaa
     1801 tgtagttgtt gaaaaaggtt tgggaacgat catagagcca aggttcataa ccagtgcgat
     1861 acggagttgt tgcggggtta gtgaaactag tgaaccagtt atttagctga ttcacggttt
     1921 ctgtaccgag gtaatcactg ctaagtggct gttcgttagg ggcgatcaac gaacaagcaa
     1981 gccaacgaga cggcaatgtt gcgtagacaa gcggttcttg aacgttgtcg gccgactgaa
     2041 agctgccttc ggttaccaat cggtagcccg ttcgaacatt cgcttcagct aaccgtgcag
     2101 tgcttgggtt cgcgtttagt tcgacgacat atggtatcgt agcagtgcta gtgaaactac
     2161 gagtgaattg aacgaacaca ctacgcactc ctggagctct cgctggatca agtccgttgc
     2221 aaagcataga ttcaggcggc atttcggtcc agttgcctag cgattttatg aataccggaa
     2281 tttcaatgcc agtttcatcg cgcacgacga tttgactagt gtcgtagaga acgccaggag
     2341 gcaatggtac accgaagcta acaagcgtcg attgtccgat ggtttcagca tgcggcgtaa
     2401 gattgactgt gacctggatt acgtcgctag gagctggcgt tgggctcggc gtggccgttg
     2461 gagccggagt agctgttggt gctggagtcg cactcggaac cggcgtagcg cttggtgctg
     2521 gagtaggtgc cggcgtagga tctggtgtcg gcgttggatc gggagtcgga ccaggcgttg
     2581 gagctggcga cggagttggt gtcggagtcg gatctggtgt cggactaggt gtaggtaccg
     2641 gagttggtgc tggttttgta aattcgcttt cgaatgcacc aatgtcataa gcgtttccgc
     2701 gaggacgttg aacgtcgtcg aagtcagaat ctgcgacacc gctagacagc gtcatgccgt
     2761 tatcgaccgc ggggctgttt tcaacaagac gatagttgta aattgttggg ttcacgaaaa
     2821 ttgaattaag agagagtccg tttgtggcga tcgaagcgcc gtcttgtgca ctttcaccct
     2881 gccactgcgc gagagtgatt gtgcgattcg acgcgtatac gttgtcgaga atgttattgt
     2941 tcgaacgtat aaataagcga tctgaatcgg aaactagttc gagtccgaca ccattaggat
     3001 gataaactaa gttgtttgtg tgattgttta agccagttga attagaaaac tgaatggccg
     3061 cacgagtcga gttttgatgt agaatcgtgt tgtgcactac cagcatgtca cgaggtcctt
     3121 cagctccgtc gatttggaag ttcgtgattc cgccggctga gttgccatac aatacgttgt
     3181 ttctaactac cgagttttgt acgccatcga gattaatggc cgcaccgcct cgggcaccgt
     3241 ttccgtagag tgtgttattt tcaatcgttg cgccgcgagt gatgccaaaa acttctggcg
     3301 tccatccggt cggaggggcg aaaaaacggt cagcgttaat ttgaattgcc gactgtccgt
     3361 tagcgaaaat tgtgttgccg cgggccgtag gtctttgatt ggagtttgaa aagtaaactc
     3421 cgtgttgttc tacggtaccc gatacggtgt tattttcgaa aactacatcg tcggcgaagt
     3481 cagtaaaaaa gccccattta ccattgttgg caaaagtcga attccgaact tgaatgaagc
     3541 gactagtgct tacgcgcatt cctgagcgaa cggcgttgct tgatctgaga ttttcgaaaa
     3601 cgagatgctc tgaccattca acaataatgt tgtcgcgatc gtaaatggca ccacgatcgg
     3661 tagagggcac aatgttgact gcgttaccag taccacgaac aacgattggt tgttcggcgg
     3721 tgcctgttaa attggatata ctgaatccca gatagtcgcc gttggccacg taaattgtgc
     3781 tgcctggtgc tgcgaacacg agagctcttc gaacctgacg acaaggcgca ctttgggtac
     3841 agaggtttgc atcgtcaccg gtggggtcaa cgtaaagcga agtgacaggt tccggtgtgg
     3901 gtgttggcgt aggaactggt gtaggtgccg gcgttggaac tggcgtagga gccggagttg
     3961 gagccggagt tggagtcggt gttggtgctg gagtcggcgc cggtgtcgga gcgggcgtcg
     4021 gagtaggatc tggagtaggt accggagtcg gaactggagt tggatctgga gttggatctg
     4081 gagtcggtgc cggagtaggt tctggtgttg gtactggggt tggaactggt acttcactta
     4141 tgccatataa aacgacctga gcactatcga ccgtaaactg tacaggggcg ccgccttctt
     4201 gagttaaacg aatccgcact cgttcgagcg ttttggttcc gtcgagttcg agcatggtag
     4261 ttaccttgcc cttgtcgcca aggttggctt gaatttcttc gcaccggctc gtggcactcc
     4321 cgtcggcgta gtttatgtaa ccacataata gaagttttga ggcgctcgca agatcagagc
     4381 gaaaatgagc tgatatttct agtctagaag cgtgttgaag gtgagcttct tctacttgct
     4441 tggcccacca aatgctgtca ccccatattt gagtgcgaat ttgaatactg tgggctcccg
     4501 ctagttgcag gcggtctggt gcagctgcgt ccggcaacca gatcacggca gtagtttctt
     4561 gatcggcctc gaagcccgat accccagttt caaaacccgg gtcggcgaca aggttgccgc
     4621 tgtttcggcc aaaaaatggt tctacgagtc tgacaacttc ttgggagtta ttctgaatct
     4681 cgtcggccac ttcggtgaag gaacaaccag tgttgaacaa cgctatgctc gcgataagtg
     4741 cgaagacata gccggactta cgagaaacaa acatggaggc ccccctcgtt gccgcgttca
     4801 agcgacaaga atcgaaactg cagttgattt gtttatttct cggtcaatac tgttaagact
     4861 tgaggcaaaa aatttcgagt aggggttttg acgtaggtat gttttgccgg tttt
//
LOCUS       DAOMXO010000084         4906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9222_length_4906_cov_4.407957, whole genome shotgun sequence.
ACCESSION   DAOMXO010000084 DAOMXO010000000
VERSION     DAOMXO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9222_length_4906_cov_4.407957"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLH57_02795"
     CDS             complement(<1..428)
                     /locus_tag="PLH57_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01566.1"
                     /translation="MRTKLFLLLFLFSFPLNAFEKILLHFDQLEAFVVRETLGEKSEW
                     LTLTPFSSDAYPAPGQAVPLLADTANVEVAVNANFYREDQPGFLKPIGLVVKNGEILS
                     EPHPNWPSAGWSKKGWIWDQVSIQATFELIDSRGTVLKTKL"
     gene            complement(418..1215)
                     /locus_tag="PLH57_02800"
     CDS             complement(418..1215)
                     /locus_tag="PLH57_02800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK01567.1"
                     /translation="MTTYNAPRHLALALAGFQIQTENNFELHIADDGSTSETSDLVEQ
                     SMQRMPFPVIYSWQQDLGYRKTKILNEAIRKAKSDYFIFIDGDCIPHRDFVKNHLKYR
                     QAGYYLAGRRVELGPHYSARITPDQVLKGALNRPNLGLLLSCLRDDSAYWHRSLPIYN
                     STLRHLLKMDHVDDLKGCNFSVDRESLFKVNGFDEDYEGYAREDTDLEVRLQYLGLKI
                     KSLKGLAIQYHVWHDRLPESEGNKLKLRELGGSRVAACRNGIVKGAN"
     gene            complement(1258..2160)
                     /locus_tag="PLH57_02805"
     CDS             complement(1258..2160)
                     /locus_tag="PLH57_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01568.1"
                     /translation="MLISAFTIVRNGVRFDYPFLESIRSVLPICEELIINVGDSDDST
                     EQALHQMIAGLSEIDQKKIQIFNTHWPLNDPEKRKGGQILAEQTNYALSKCSGQWCLY
                     LQADEVLQEDDLSIISQAAEAHLNDDRVDAFVFEYVHFYADYTTIQTSRSSYRREIRM
                     VRRSSGAVSVGDAQSFRLPDGTKPRAVLLKAKIYHYGWVKNQDVMRLKTGFMDTLYHQ
                     NADAALPATGPNYLFKSFVGLKKFTGRHPGVMKTRVEAAGNFDFSRAPRVFQIKDVWK
                     IVSGWIERATGWRPFEYKSYRLIR"
     gene            complement(2162..3232)
                     /locus_tag="PLH57_02810"
     CDS             complement(2162..3232)
                     /locus_tag="PLH57_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRK01569.1"
                     /translation="MKNTHRRILAFKTRAIGDTVLLTGILRVLRAHRPNWELHALVSS
                     EAGPLLEGLPFVDRVIGVTEPKSKTDRLAFWLKMLRRLRSIRYDQVLNFHASFRTALV
                     SQLLRTELCVANDHSLASKNRLSDLPVPGRGRVKSIIDRDLDVLRSIGISATVDDALP
                     ELKPTTYEIEEAKKLLGRDKSGGTYVFLGVGGSRGVKRWPAEHFLVLMEKLSVQSNHC
                     FVLATVRSDEEWLSSFFKMLKDKPDLQKRILHFKNLPIRMAMAVLSQCDRYIGNDSGL
                     KHIAAAVGVPTHTFFGPEAPLEWHPYPTSKHTYSFIEDLACRTETGKHWCPIETCERH
                     GHKCLKGITPDEVATLLKGSSK"
     gene            3368..4495
                     /locus_tag="PLH57_02815"
     CDS             3368..4495
                     /locus_tag="PLH57_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01570.1"
                     /translation="MESMLPIVDELVINVGKSEDGTEDLMRQLQAANPSKIKLHFNTW
                     DDQQTKDGLVLSVQTNLALAHCRGRWALYLQGDEALHESELRAIREAVEKADGLAVND
                     QPEAFELRYLHFYGGYSLIQQPWNWYPTEIRIIRRDSGAQSFGDAQTFRRSNGDKLST
                     QLLDAHVFHYGHARAPETMARKNHYFHRFWHGDGHGKKTNEAYPIDLRNLVWYWGTHP
                     AVYAERIRLGVAWSPTPKSVIAPHWETVSVVYSKKDTKQVLLAETLARELRHRGHSVK
                     LICSTFSWLKSVYTTWISRHRNALIDLNTTHRGILWFPCWAGDALFAYSLRIAHAPDG
                     RLSKFRKTFFNSVHWGHHENKNGFAVPEAKWPEQLLKYLGL"
     gene            4574..4861
                     /locus_tag="PLH57_02820"
     CDS             4574..4861
                     /locus_tag="PLH57_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01571.1"
                     /translation="MNSSAKKLFVGALMSGLIAGTAALAENAPAAAKKGKAVASKKAK
                     NCACGKKSKDCKCDKNGCGKEGCGKKDHDHSDHKDSCGKNGCESKSEDKEE"
BASE COUNT         1336 a         1172 c         1124 g         1274 t
ORIGIN      
        1 agttttgttt tcaaaacagt tccgcgactg tctattaatt caaatgttgc ttgaatgctg
       61 acttgatccc aaatccaacc ttttttcgac caacccgccg acggccaatt ggggtgtggc
      121 tcagaaagta tttcaccatt ttttacgacc agaccaatcg gtttcaaaaa acctggctgg
      181 tcttcacgat aaaaattggc attcactgct acttcaacat tcgctgtatc cgctagcagc
      241 ggtactgcct gaccaggcgc agggtacgcg tccgaagaaa aaggagtgag cgtcaaccat
      301 tcagattttt ccccaagcgt ctcccgaaca acaaacgcct cgagttggtc aaagtgaagt
      361 agaatttttt caaatgcgtt aagaggaaaa gaaaaaagaa acagaagcaa aaataattta
      421 gttcgcacct ttgacgatcc cgttacgaca ggcggcaaca cgcgacccgc ccagctctct
      481 taatttaagt ttgttccctt cactctccgg aagtcgatca tgccaaacat gatactgtat
      541 cgctaaaccc ttgagtgatt taatttttaa accaaggtac tgcagacgca cttcaagatc
      601 ggtatcttcg cgtgcatatc cctcgtagtc ttcgtcgaat ccgttcactt taaagagtga
      661 ttcacgatct acagagaaat tacagccctt caggtcatct acgtgatcca ttttgagcaa
      721 atgacgcaaa gtcgaattgt atatagggag cgaacgatgc cagtatgcag agtcatcacg
      781 caggcaactg agtagcagac caagattcgg gcgatttaat gctcccttta aaacttggtc
      841 gggcgtaatg cgggccgagt aatgtggccc cagctctacg cgacgtccgg ccagatagta
      901 gcccgcctgg cgatatttaa gatgattctt aacgaagtcg cggtggggaa tgcagtctcc
      961 gtcgatgaag ataaaatagt cggatttcgc cttacgaatg gcctcattta aaattttggt
     1021 tttgcggtat cctaggtctt gctgccaaga gtaaattacc ggaaacggca tgcgttgcat
     1081 gctttgttcc acaagatcag aagtttctga agtactgccg tcgtcggcaa tgtgtaattc
     1141 aaaattgttc tcagtttgaa tttgaaaccc cgccaacgcc agcgccaaat gcctaggcgc
     1201 gttgtaagtg gtcatgatta cgctcgcgag aggtttttca ccttttttgt acatagttca
     1261 ccgaattaac cgataacttt tatattcgaa cggacgccag ccagtggctc gctcaatcca
     1321 gccagaaacg attttccaga cgtcttttat ctgaaatacc ctaggggcgc gagaaaagtc
     1381 aaagttcccg gcagcctcta cccgagtttt catgacgcca ggatgccgac ccgtaaattt
     1441 tttcaagcct acgaaacttt taaaaagata attcggcccc gtcgcaggga gcgcagcgtc
     1501 ggcgttttgg tgataaagcg tgtccataaa gccagttttg agccgcataa catcttgatt
     1561 ttttacccag ccgtaatgat aaatcttcgc tttcagtaat acggctctcg gcttagttcc
     1621 gtccggtaat cgaaaactct gggcatctcc caccgaaact gcgcccgaag agcgtcggac
     1681 catgcgaatt tcgcggcgat atgacgagcg tgatgtttga atcgtagtat agtcggcgta
     1741 aaaatgaacg tactcgaaaa caaaagcgtc tacccggtca tcatttaggt gcgcttccgc
     1801 agcttgacta attattgaaa gatcatcttc ctgcaacact tcgtcagctt ggaggtacaa
     1861 acaccattgg ccgctacact ttgaaagagc ataattggtt tgctccgcta aaatttgacc
     1921 accctttctt ttttcggggt cgttcaatgg ccagtgggta ttgaagattt gaattttttt
     1981 ctgatctatt tcgctaagtc ccgcgatcat ttgatgaagc gcctgttcag tagagtcgtc
     2041 ggagtcgcct acgttgatta tcaactcttc gcatatcggc aagacactcc ggattgattc
     2101 taaaaaagga tagtcgaatc gaacgccatt gcgaacaata gtgaatgcac ttatcaacat
     2161 gttactttga cgaccccttt aatagagtcg ccacttcatc gggggtaatg ccttttaaac
     2221 atttgtgtcc gtgtcgctcg caggtttcaa tggggcacca gtgcttgcct gtttcagtgc
     2281 gacaagcaag gtcttcaata aatgagtatg tatgtttaga tgtagggtat gggtgccact
     2341 ccagtggcgc ctcaggtcca aagaaagtgt gggtcggaac accaaccgcc gcagcaatgt
     2401 gtttaagtcc tgagtcgttt ccaatgtacc gatcgcactg cgaaagcact gccatcgcca
     2461 ttcgaatcgg gagattttta aagtgcagaa tgcgtttttg aagatcaggc ttatctttaa
     2521 gcattttaaa aaaactagaa agccactctt catccgaacg gacggttgct agaacaaaac
     2581 aatggttaga ttggaccgat agtttttcca tcaaaactaa aaagtgctcg gcaggccagc
     2641 gttttacccc tcgacttccg ccaactccta aaaatacgta agtccctcca cttttatcgc
     2701 gtcctaaaag tttttttgct tcttcgattt cgtaggtcgt cggctttaat tctggtaggg
     2761 cgtcgtcgac tgtagcggag attccgattg agcgaagcac gtcgagatcg cggtcgatga
     2821 tcgacttcac ccggcctcgg ccaggaactg gaagatccga caatcggttt ttggaagcca
     2881 gcgagtggtc gttcgccaca cacaactccg ttcgtagcaa ctgcgaaacc aatgcagttc
     2941 gaaaagacgc atgaaaattt agaacctgat cgtaacgaat gcttcgaagg cgccgcagca
     3001 ttttcaacca aaatgcgagt cgatccgttt ttgatttcgg ttcggttacg ccaatcacgc
     3061 ggtctacaaa aggcaaacct tccaataagg ggcccgcctc cgaactcacc agggcgtgca
     3121 attcccaatt ggggcgatgg gcacgcaaaa cccgtaatat tcctgtaaga agcacggtgt
     3181 ctccgatggc ccgagttttg aacgctaaaa ttcgacgatg tgtgtttttc atgttgtgtg
     3241 tttcataaat gaaagacttt ccaacacccg cgttccgcgt tagatggtgg ataattctac
     3301 tatgctttta agcggcttca cctttattcg aaatgccata aaattcgact ttccgatacg
     3361 ggaatgtgtg gaatccatgc tacctatcgt cgacgaattg gtcatcaacg ttggaaagtc
     3421 tgaagacgga actgaagatt taatgcgtca actccaggcc gcaaacccta gcaagattaa
     3481 actgcacttc aatacctggg acgaccaaca aacgaaagat ggacttgttc ttagcgtgca
     3541 gaccaatctt gcgctcgcac attgccgagg tcgctgggca ctctatttac aaggagatga
     3601 ggcgctgcac gaatccgaac tccgagcaat tcgagaagca gtagaaaagg ccgatggcct
     3661 cgcagttaac gatcagcctg aagctttcga actccgctat ttacattttt acggcgggta
     3721 ctccttaata caacaaccat ggaactggta tccgaccgaa attcgaatca ttcgacgaga
     3781 ttcgggggct caaagttttg gagacgccca aacttttcga cgcagcaacg gggataaact
     3841 ttcaacccag ctgctcgatg ctcacgtttt tcactacggt catgcccgtg cccccgagac
     3901 tatggcccgt aaaaatcatt actttcaccg cttttggcat ggcgatgggc atggtaaaaa
     3961 gaccaacgaa gcttatccga tagaccttcg aaatctcgtt tggtactggg gtacccaccc
     4021 tgccgtgtac gccgaacgaa taagattggg tgttgcctgg agcccgactc caaaatcggt
     4081 cattgctccc cactgggaga cggtgtcagt ggtttactcc aaaaaagata ccaaacaggt
     4141 tcttctagcc gagacactag cgcgagaact ccgtcatcgc ggtcattcgg tgaaactgat
     4201 ttgctcgacc ttctcttggc taaaatcggt ttatacaacc tggatatcta ggcaccgcaa
     4261 tgcgttaatc gatcttaata ccacgcaccg cggaatttta tggtttccct gttgggcagg
     4321 cgacgctttg ttcgcatatt cgttgagaat tgcccacgcg ccagatggtc gcctctctaa
     4381 gtttcgcaaa acttttttta attcggtgca ttggggacac cacgaaaata aaaatggatt
     4441 cgcagttccc gaagctaaat ggcccgaaca gcttttgaaa tatcttggct tgtagttctt
     4501 ttttggcaaa atggccaaat ctccaatact cggagaatta cgttcgaaac ctgttactaa
     4561 aaaaggaaaa aaaatgaact catcagcaaa aaaattgttt gtgggcgctc taatgagcgg
     4621 tctcattgcg ggaactgctg cgcttgcgga aaatgccccg gcagctgcca aaaaaggcaa
     4681 agccgttgct tcaaaaaaag caaaaaactg cgcctgtggt aaaaaatcca aggactgcaa
     4741 atgtgacaaa aacggatgtg gtaaagaggg ctgtggaaaa aaagaccacg atcactccga
     4801 ccacaaagat agttgtggaa aaaacggttg tgaatcgaaa tctgaagaca aagaagagta
     4861 gataatgagg gggctggctt ttcgtaaaag ccagcccttt tatttt
//
LOCUS       DAOMXO010000085         4854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9342_length_4854_cov_4.872682, whole genome shotgun sequence.
ACCESSION   DAOMXO010000085 DAOMXO010000000
VERSION     DAOMXO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9342_length_4854_cov_4.872682"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLH57_02825"
     CDS             complement(<1..644)
                     /locus_tag="PLH57_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HRK01572.1"
                     /translation="MKDYSNVIKKADLKKLRPYGDIMDDGVVQVSFTLPVEASPEARE
                     AATQLAGKMGLTEVKIATMEIAAPGFSFFVIYGKLAHTVDFTSIKVIKVESPKLSMKE
                     VDELIKEKLGRKLVILGACTGYDAHTVGIDAIINMKGFAGDYGLERYQWLDARNLGAQ
                     VLNEDLLKKAVETNADVILVSKVVTQHNIHIKDMEDLIKKAEKMGLRDKILFIAG"
     gene            complement(641..2206)
                     /locus_tag="PLH57_02830"
     CDS             complement(641..2206)
                     /locus_tag="PLH57_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HRK01573.1"
                     /translation="MAHLELNQQTIIECRKLANQIVAKVKTVIDVHTSVSIERAALRL
                     IGVEGAIQKNGLNYPLVNEIVDQLRSDRVIAKGALYWFANGLLASGLETNELALKVGA
                     GEIKLSQLDRASLTDIRKKAQELCKAALNRLKTKREERADLRKSQGDPFDTTWNGTPL
                     KYVIVATGNIFEDAVQAKAAVEAGADAIAVIRSTAQSLLDYMPEGATTEGFGGTYATQ
                     ENFKIIRKALDEAGEKAGRYIRLTNYSSGLCMSEIAAAAAIERLDFLLNDAMYGILFR
                     DINMKRTLIDQNFSRMIIAYSDITIHTGEDNYLTTADAVENGHQVLASQFINERFALH
                     AHMREDQMGLGHAAEMDPWHPNVLLLEIARAQMTRDIFPKSPIKFMPPTRHKVGDIFF
                     SHLMDAMFNFVGVLTNQGIQLLGMPTEAMHTPLLQDRWMSIKNANYIFGAAKDLGKEI
                     TFKRDGTISKWANKVLDDTLVLLRKIDRDGLFKAIEDRAFANVSRSETGGKGLDGVVQ
                     KDKEYTNPFFELL"
     gene            complement(2193..3236)
                     /locus_tag="PLH57_02835"
     CDS             complement(2193..3236)
                     /locus_tag="PLH57_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HRK01574.1"
                     /translation="MGGNCFGTHRSKEPVGSLPQQATVLDASLPIYENELLIEVESLN
                     IDSASFHQIKSVCGSSLPSMESHILNLVRSQGKHQNPVTGSGGMLIGTVADVGPKFEF
                     KDGNLKKGDRIATLVSLTLTPLHIEKIKKIHLNLDRVDISGHAVLFTTGIYSKLPSDI
                     PESLALAILDVCGAPAQTAELCKPGDTVVVIGGAGKSGVLCLYEAAKAVGPKGKVIAL
                     DYSTEAVNGIRSLSFVKNAFAVDARDALAVYEIVNKATDGKMADLVINVANIPDTEMS
                     CVLASKQGGVAYYFSMATSFTKATLGAEGVGADVELIMGNGYRPGHADLALNILRQAP
                     EIRKMYETKYGAS"
     gene            complement(3263..4318)
                     /locus_tag="PLH57_02840"
     CDS             complement(3263..4318)
                     /locus_tag="PLH57_02840"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRK01575.1"
                     /translation="MNLFERISNDGYEQIVFCHDKDVGLKAIIAIHDSTLGPGTGGCR
                     MYPYATEDQALEDVLRLSRGMTYKASISGLKLGGAKAVIIGDPKKDKTPELLKRFGQY
                     VETLKGRYITAKDVGINGDDLALVRRETNHVLGIEGVAGSSGDPSPLTAWGTYNGMKA
                     CAAHVWGTSSLKGKRIAVQGLGYVSFSLIGYLVKEGASVVGADVDPQAVARAQKEYGI
                     DIVAPNEILAQDCDIVSPNALGAILNNLSIRELKCKVVAGAANNQLATDEDGAELMRR
                     GIVYAPDYAINAGGLMNIYHELDGYNAERAYDHVGGIYKTIEDILVRAKKENQPTQRI
                     ADRMAEERIQNAKKKRS"
     gene            4446..4652
                     /locus_tag="PLH57_02845"
     CDS             4446..4652
                     /locus_tag="PLH57_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRK01576.1"
                     /translation="MALGSVKWFNDAKGYGFIQQDGSERDIFVHYTAIHGDGFKTLAE
                     GQKVEFELVEGPKGPQATKVQKVQ"
BASE COUNT         1229 a         1192 c         1001 g         1432 t
ORIGIN      
        1 ccggcaataa ataaaatttt atcccgaagt cccatttttt cagctttttt tatgagatct
       61 tccatgtctt tgatgtgaat attatgttgg gtcactactt tagaaacaag aattacatct
      121 gcattcgttt cgacagcttt ttttaggaga tcttcgttta gaacttgtgc tcccaagttc
      181 cgtgcgtcga gccattggta tcgttcgagc ccgtaatctc cggcaaaacc tttcatgtta
      241 attatagcgt cgatacctac agtatgggca tcatatcccg tgcaagcgcc gagaataact
      301 aattttcgac caagcttctc tttaatcaac tcgtcgactt ctttcatcga tagttttggt
      361 gattccactt taataacctt gatagacgtg aaatccacag tgtgagctaa tttcccgtat
      421 attacaaaaa acgaaaaccc tggtgctgca atttccattg tagcgatttt tacttcagta
      481 agtcccattt ttcccgcaag ctgcgtggcg gcttcacgag cttctggcga agcttcaacg
      541 ggcaatgtaa aggacacctg tacgactccg tcgtccataa tatcgccgta gggacgtaat
      601 tttttcaaat cggctttttt aataacgttg gaataatctt tcatagaagt tcgaagaatg
      661 gattagtata ttctttgtcc ttttgaacaa cgccgtcgag cccttttcca ccagtttcac
      721 tccgcgatac gttcgcgaaa gctcgatctt caatggcctt gaataaaccg tctcgatcaa
      781 tttttcgaag taaaactaac gtgtcatcta atactttgtt agcccactta gatattgtgc
      841 cgtcgcgctt aaaagttatt tctttaccaa gatctttggc cgcaccgaaa atgtaatttg
      901 catttttgat gctcatccat cgatcttgta aaagtggcgt gtgcattgct tcggtcggca
      961 tgcctaaaag ttgaatccct tgattagtta aaacgccaac aaagttaaac atcgcatcca
     1021 taagatgact aaagaaaata tctcctactt tatggcgagt tgggggcata aatttaatag
     1081 gcgattttgg aaaaatatca cgcgtcatct gggctcttgc gatttccaat agaagtacgt
     1141 tcgggtgcca tgggtccatt tcggccgcat gtcctaatcc catctggtct tcgcgcatgt
     1201 gagcatgaag agcaaaacgc tcgttgataa actgcgaggc caacacttgg tgaccatttt
     1261 ctacagcgtc ggcggtggtt agataattgt cttccccggt atgaattgta atatctgaat
     1321 acgcgataat catacgcgaa aagttttgat caattaatgt tcgtttcata ttaatgtcac
     1381 gaaacaaaat gccgtacatc gcgtcgttaa gtagaaaatc gagccgttcg atcgctgctg
     1441 ccgctgctat ttcagacata catagaccag aagaataatt ggtcaatcga atgtagcgac
     1501 cagctttttc tccggcctca tcaagcgctt tgcgtattat cttaaagttt tcttgagtcg
     1561 cgtaggtccc gccaaatccc tctgtcgtag caccttcggg catatagtcg agtaaacttt
     1621 gagcagtaga tctaattacg gcaatggcat ctgcccccgc ctctacggcg gctttagcct
     1681 gaactgcgtc ttcgaaaata ttaccggtcg caacaataac gtattttagc ggtgtaccgt
     1741 tccaagtagt atcaaacgga tcaccctgcg acttacgcaa gtcggcgcgt tcttctctct
     1801 tagtcttcag gcgattcaga gctgctttac atagttcttg ggcttttttc cgaatgtccg
     1861 tcaggctcgc tcggtccagc tgacttagtt taatttcccc tgcgccaacc ttaagggcaa
     1921 gttcgttagt ttcgagtcct gacgctagaa gaccgttcgc aaaccaatac aacgcgccct
     1981 tggcaattac tcgatcgctg cggagctgat ctacaatttc gttcactagt ggatagttta
     2041 agccgttttt ttgaattgcg ccttcgactc ctatcaacct aagtgccgcg cgttcaatac
     2101 ttacactggt atgcacgtca atgactgttt taacttttgc gacaatttga tttgccagct
     2161 tacgacattc gataatggtc tgttgattca gttcaagatg cgccatactt agtctcgtac
     2221 atttttctaa tttcaggcgc ttgtcttaat atatttagcg ccaagtccgc gtgtccgggg
     2281 cgatatccgt tacccataat tagttcaacg tccgctccaa caccttcggc tcctagggta
     2341 gctttcgtaa agctggtcgc catactaaaa taataagcca ctcccccttg cttggaggcc
     2401 aacacacacg acatctccgt atcaggaatg tttgcaacgt ttattactaa atcagccatt
     2461 ttaccgtcag tcgctttatt cactatttcg taaactgcaa gcgcatcacg ggcatctacc
     2521 gcaaatgcat tttttacaaa tgaaagtgag cgaataccgt ttactgcctc cgtgctgtaa
     2581 tcgagcgcga tcacctttcc ttttggtcca acggcctttg cggcttcgta cagacacaat
     2641 accccggatt ttccagcacc accaatcacc acaaccgtat cgccgggttt gcaaagttca
     2701 gcggtctgag cgggcgcacc acatacatct aaaattgcta atgctaaaga ctcaggaata
     2761 tccgatggta atttcgaata aataccggtc gtaaacaaga ctgcgtggcc cgaaatgtct
     2821 actcggtcca aattcaaatg aatttttttt atcttttcga tgtgcagcgg cgtcaatgta
     2881 agtgaaacta aggtcgcaat tcggtcgccc ttttttaaat ttccatcctt gaattcgaac
     2941 ttaggtccaa cgtcagccac cgttcctatg agcattccac ccgatccggt tactggattt
     3001 tgatgtttgc cctgactgcg gaccaaattt aaaatatgcg attccatgct tggcagacta
     3061 gaaccacaaa ccgacttaat ttggtgaaaa ctcgcggaat cgatatttaa cgattctact
     3121 tcgattagaa gttcattttc ataaatgggc agtgaagcat cgagcaccgt tgcctgctgt
     3181 ggcaaagacc caacgggttc tttcgaacgg tgggtaccaa aacagttgcc ccccattcga
     3241 tacttctgaa cggttcccaa aactaggacc tctttttctt agcgttctga attcgctctt
     3301 ccgccatgcg gtcggcaata cgttgagttg gttgattttc tttcttcgct ctaaccaaaa
     3361 tatcttcgat ggttttgtaa atacccccaa catgatcata ggcccgttcg gcattgtagc
     3421 cgtcgagttc gtggtagata ttcatgagtc cgccagcatt gatggcatag tcgggtgcgt
     3481 acacgattcc gcgccgcatt aattccgcac cgtcttcatc ggtggccaat tggttgttag
     3541 cagctccggc aactacttta cacttcagtt cgcgaatcga aagattattg agaatagcac
     3601 ccagcgcgtt aggagaaaca atgtcgcaat cctgagccaa aatttcatta ggtgctacga
     3661 tgtctattcc gtattccttc tgcgctcttg ccacagcctg agggtcaacg tcggcaccta
     3721 caactgaggc gccctcttta accaaataac caatgagcga aaaactaaca taacccagtc
     3781 cttgaactgc aatacgttta cctttgagcg aagaagtacc ccaaacgtgt gcggcacagg
     3841 ctttcattcc gttgtaggtt ccccaagcag tgagcggtga tgggtcgcca cttgaacctg
     3901 caacgccttc aatccccagt acgtgattgg tctcgcgacg caccagtgcc aagtcgtcac
     3961 cattgatgcc cacgtctttt gcggtgatat agcgaccctt gagggtttcg acatactgcc
     4021 caaatctttt caaaagttcg ggagttttat ctttttttgg atcaccaata atgacggctt
     4081 tcgcaccgcc aagcttcaag ccgctaatcg acgccttgta agtcatgccg cgagaaagtc
     4141 tcaaaacgtc ctctaaagct tgatcttcag tcgcgtaggg atacattctg cagccgccgg
     4201 tgccgggccc aagggttgaa tcgtgaatag cgataattgc ttttaggccc acgtctttgt
     4261 cgtggcaaaa tacaatttgt tcatatccat cgttgctaat tcgctcaaaa agattcatct
     4321 cggtatgtct cctctttctt tactcaaacg ttacgcagat ctatagcgca tttgatattg
     4381 acactcaact aacgagaacg ataaacctac gatatagatc attattttta gggggattac
     4441 gtattatggc acttggatct gtaaagtggt ttaacgacgc taaaggctac ggttttattc
     4501 aacaggacgg tagcgagcga gacatttttg ttcactacac tgctattcac ggggacggct
     4561 tcaaaaccct tgctgaaggc cagaaggtag agtttgagct tgttgaaggt ccgaaaggtc
     4621 ctcaagcaac caaagttcaa aaagttcagt aaatcacagc tctttgttcg taacaaagcc
     4681 gccaccccac gtggcggctt tgtctttttt aaaacttgaa aaacccaatt cattacgcct
     4741 gtctcgtagt aattttggct cttgcggcgc tggttacggc gcttcctcac ttcgaactta
     4801 agtccaatcg gtacatgccc ttctataact tacaggctga cgcctttttg cacg
//
LOCUS       DAOMXO010000086         4844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9374_length_4844_cov_6.002088, whole genome shotgun sequence.
ACCESSION   DAOMXO010000086 DAOMXO010000000
VERSION     DAOMXO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9374_length_4844_cov_6.002088"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLH57_02850"
     CDS             <1..424
                     /locus_tag="PLH57_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01577.1"
                     /translation="PPFLNKELAIGVSQQGLWNWDVVEVFLQPNVSSSTYYEFVVSPL
                     GQHFELEVFEPRRRVNPLFRSGARISAVMGTGGWRSSIKVDLRKIGWAPLTGRLKWNC
                     FAILGQPDSAPGRSYWSLNLPEQTEPDFHLPQYFTALD"
     gene            complement(408..1655)
                     /locus_tag="PLH57_02855"
     CDS             complement(408..1655)
                     /locus_tag="PLH57_02855"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRK01578.1"
                     /translation="MPQPLLEFCSEFGRAYLASGGPTSRLEEYVTELGRVYEHPTEVF
                     ATPTGVFVSLVDKDGMHRTTLSRVKQVSMNLGRLCWLEGIFEDITTKKITLSQGLRVL
                     GNKKVRNATYPLWVATLAAFVGGFALSLPNYGRVRAALASGLIAMVTWWISGPGLNRR
                     IQSSIFRDFIGCTAALALAATAQFFIPGPFEAFAIGALIVLVPGLALTSAISELADQN
                     LVSGTAKLMQAILTLLALGISYLLFTDLVSGLGLHSFPMISAERRPLSLGASAVGAAF
                     GVTCFGILFGVPRKALPGATATGLLGWTVFHQFDNTKLLVTAPFLASTAVGFVSLVLS
                     NRFKVPSQTYSVPGILAMLPGMLALASFHTLALGNETVGIELGFRVATTAAAIVFGLF
                     AARIPFTLWQVTRIRLKKVSPRL"
     gene            1730..2017
                     /locus_tag="PLH57_02860"
     CDS             1730..2017
                     /locus_tag="PLH57_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRK01579.1"
                     /translation="MMSEWYSLEADAKHISKERARAQKLKASQWWRTKLNQGVCHYCE
                     AQFKPADLTMDHVVPVARGGKSQPGNVVPACRACNASKKLHTPVDLLLGGR"
     gene            2021..2716
                     /locus_tag="PLH57_02865"
     CDS             2021..2716
                     /locus_tag="PLH57_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01580.1"
                     /translation="MERRAFFQELVERAGKTGMFARFFSNEKTIDQAALEARLMRDED
                     YYESVKSRFVMRSGEAVTLNGEPFGRWVSDLVVMERPDPMGETENSSLVIVCGHEHEG
                     LSVFEWSRGQHAISSYVNARISEKTPVAVWDDKSEELGAFGITHIVYEPKSLNEPGYV
                     HALERDGHVIELNPHTGVFFRRRNLSFHLAAIPWLRWPNGDKCLVRVTESKKFAVVAF
                     VNADGQSFVSVVT"
     gene            complement(2689..3810)
                     /locus_tag="PLH57_02870"
     CDS             complement(2689..3810)
                     /locus_tag="PLH57_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01581.1"
                     /translation="MIRILLLLGLSTSLSAAQNREPSYTTAGRKVDSAYADYRSELET
                     RIKQVHAACRTYNRVLCKPLTPTPVRPAPKGYQVLPSITVNLPRAEPPKHPRLEKTFY
                     SWPRTLAFIERERAIMDEIHQQLGSLSKLSKPDARVVLKQIVAGALKLRQAHTVIDEH
                     VKYNWFWQSRVDSQQSSFTKAAALLARAEKSPRDVDLARMFDVQKPQYIKRIGSTFSV
                     PVVTDISDPAFIAVFKKAVEEEWRTEHYQVKLDIRTFSPKLPPGFDPSQNLDAHLQLF
                     PSNAAVLTTGALRSFERLGSAIVLGPERLTPRELAHKFGHILGFPDFYLRAATDLGSS
                     GYEIIEAVIETDDLMAEPQRGRVRASHFEQLQVTTETKL"
     gene            complement(3810..>4844)
                     /locus_tag="PLH57_02875"
     CDS             complement(3810..>4844)
                     /locus_tag="PLH57_02875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRK01582.1"
                     /translation="TADEGFRTELTARFLFVATGSRPSETDVLKADHEHILTTDDIVL
                     MRELPDTLTIVGGGVVACEFASILSKFGVRIHLLEHSNQVLSAMDPEIVQLLMQRFSK
                     EGIRIHLHSKVAKVEVREDGEQAVKLHLDVQNKTETLLTDKVLLAIGRKPNSDDLGLE
                     TIGVQTTPRGHILVDDRLETHCRGLYAGGDVVGGQMLAHKAWYDAGIAVGAMCGSEAK
                     TDYNTVPGAIFTIPEMASVGLQPDQARQKGINVAIGKFFYAENSQAMCIGETEGLVKA
                     VVDRDTGRVVGCTMIGHDASNLISEVALAMSAGLTANDIAETIHPHPTLSEMVWESFL
                     DTQGLSVHKQ"
BASE COUNT         1188 a         1174 c         1357 g         1125 t
ORIGIN      
        1 gccacctttt ctcaacaaag aactcgccat aggagtttct cagcaggggc tttggaattg
       61 ggacgtggtg gaagtgtttt tgcaacccaa tgtgtcttct tcaacctatt atgagttcgt
      121 cgtgagtcca ctgggacaac atttcgaact cgaagttttc gaacctcgaa ggcgcgtgaa
      181 cccgctgttt cgatctggcg caaggataag cgcggtaatg gggacaggtg gctggaggtc
      241 gagcatcaaa gtcgatcttc gaaagattgg ttgggcgcct cttactgggc gcctaaagtg
      301 gaactgtttt gcgattcttg gacaacccga ctcggcaccg gggcgcagct actggagttt
      361 aaacctaccc gaacaaactg agcccgattt tcatttaccg cagtacttca cagccttgga
      421 ctgacttttt ttagtcgaat tcgagtaact tgccacagcg tgaatgggat gcgggcggcg
      481 aaaagtccga aaactatcgc ggcagctgtg gtcgcaactc gaaagccaag ctcgatgccc
      541 acggtttcgt tgccaagtgc gagagtatga aagcttgcca gagccaacat tcccggcagc
      601 atggcaagaa taccgggaac agagtaggtt tgactgggaa ctttaaagcg attgctgaga
      661 acgagactca cgaaccccac ggcagtagag gccaaaaatg gagcggtcac caggagtttg
      721 gtgttgtcga attggtggaa aacagtccat ccgagtagtc cggtggcggt cgctcccgga
      781 agcgctttgc ggggcacccc gaaaagaata ccaaaacacg ttacaccaaa ggccgcaccg
      841 acggcggacg cccccaacga cagcggtcgc cgctcagcac tgatcatagg aaaagagtga
      901 agtcctagtc cactcacgag gtccgtgaaa aggagatagg aaatccccag cgccaatagt
      961 gtcaatatag cctgcatgag tttagcagtg cccgaaacta agttctgatc agcgagttcc
     1021 gaaattgccg aagtaagggc cagtcccgga accagcacaa tgagagcccc gatcgcgaaa
     1081 gcttcaaagg gcccgggaat gaagaactga gcagtggccg caagcgcgag ggctgcagta
     1141 cagccgataa agtcgcgaaa aatactactc tgaatgcgcc gatttaggcc ggggccggaa
     1201 atccaccaag tgaccatggc gatcaagcca ctggcgaggg cggcacggac tcgcccgtaa
     1261 ttgggcaggc tcaacgcgaa tcctccgacg aaggcggcaa gggtggcgac ccaaagcggg
     1321 taggtggcgt tgcgaacctt tttgttaccc agaacccgca acccctggga tagggtaatt
     1381 tttttggtgg tgatatcttc gaaaatacct tcgagccaac aaagtcttcc caggttcatc
     1441 gacacttgtt tgactcgcga gagcgtcgta cggtgcatgc cgtctttatc gacaagagaa
     1501 acaaacactc cggtcggggt cgcgaaaacc tctgtggggt gttcgtaaac acggccaagt
     1561 tcggtgacgt actcttctaa acgcgacgtg ggaccgccgc tggcaagata ggcgcgaccg
     1621 aattctgaac aaaattccaa taagggttgt ggcataggtg ctccgctacc caaacttaat
     1681 ctcgaaagac tcgctccggc aacccctctt gtgttataag ccggtcagaa tgatgtcaga
     1741 atggtattcg ctcgaagccg acgcaaaaca catctcgaaa gaacgtgcgc gagctcaaaa
     1801 attaaaagca tcccagtggt ggcgcaccaa attgaatcag ggcgtttgtc attattgcga
     1861 agctcagttt aaacctgccg atctaacaat ggatcacgtc gtaccagttg cgcgtggtgg
     1921 aaaatcccaa cccggaaacg tagtgcccgc gtgccgagcc tgtaatgctt cgaagaaact
     1981 tcatactcct gtcgatctgt tgttgggtgg gcgctgaaca atggaacgac gcgctttttt
     2041 ccaggaacta gtggagcggg ccgggaaaac cggtatgttt gcccgttttt tttccaatga
     2101 aaaaacaatc gatcaagcgg ctcttgaagc tcgcctgatg cgtgatgaag attattacga
     2161 atcagtaaaa tcgcgattcg tcatgcgttc tggcgaagcc gtgactttaa acggagagcc
     2221 gtttgggcgt tgggtgagcg acctggtggt gatggaacgc cccgacccta tgggcgagac
     2281 tgaaaattcg tcactggtaa tcgtatgtgg acacgagcat gaggggttga gtgtatttga
     2341 gtggagtcga ggacaacacg cgatttcgtc ttacgtgaac gcgcgaattt ctgaaaaaac
     2401 tccagtcgca gtgtgggatg acaaaagcga agaactcggt gcattcggaa ttactcacat
     2461 tgtttatgag cctaaatctt taaacgaacc ggggtacgtg cacgcgttag agcgagatgg
     2521 acacgtgatt gaattgaatc cccatactgg ggtttttttt cgcagacgaa atttgagttt
     2581 ccacttagca gcgattccgt ggttgcgttg gcccaacgga gacaagtgcc tggtgagagt
     2641 caccgagagt aaaaaatttg ccgtcgtagc gtttgtgaat gccgacggtc agagttttgt
     2701 ttcagttgtg acttgaagtt gctcaaagtg agaagccctt acgcgaccac gttgaggttc
     2761 tgccatgagg tcgtcagtct cgatcaccgc ttcgatgatt tcgtatccgc ttgatccaag
     2821 atctgtggcg gctcgaagat aaaaatctgg aaagccaaga atatgaccaa atttatgggc
     2881 gagctcgcga ggcgtgagcc gctcaggccc tagtacgatt gcgcttccca agcgttcaaa
     2941 agaacgcaaa gccccggtag tgagaaccgc agcgttggaa ggaaataatt ggaggtgggc
     3001 gtcgaggttt tgactcggat cgaagcccgg gggcaatttc ggactgaatg ttcgtatatc
     3061 cagtttcact tggtagtgct ccgtgcgcca ttcttcttcg actgcttttt tgaaaactgc
     3121 gataaacgct ggatccgaaa tatcggttac aactggtacc gaaaaagttg aaccgatgcg
     3181 cttgatgtat tgtggttttt ggacgtcaaa cattcgagca aggtcaacat ctctcggaga
     3241 tttttcagcc cgggccaaca gtgcggcggc cttcgtgaac gagctttgtt gcgagtcgac
     3301 gcgactttgc caaaaccaat tgtatttaac atgttcgtcg ataacggtgt gcgcttggcg
     3361 taacttgagg gcgcccgcta cgatttgttt gagtacaacg cgagcgtcgg gcttagagag
     3421 tttggaaagt gaaccaagtt gttggtgaat ttcgtccatt atagcgcgtt cgcgctcgat
     3481 gaaggctaac gtgcgaggcc aactgtaaaa agttttctca agtcgtggat gtttcggagg
     3541 ctcggcgcgc ggcaaattaa cagtgattga cggcaacacc tgatagcctt tcggggcggg
     3601 ccgcacaggc gttggggtga gaggtttgca cagaacgcgg ttataggtac ggcatgcagc
     3661 gtgaacctgt ttaatgcgcg tttcaagttc gctgcgatag tcggcatagg ccgaatcgac
     3721 ttttcggccc gcggtcgtat aagatggctc gcggttttga gccgcagata gtgaggtgga
     3781 aagacccagc aatagcagga tgcgaatcat cactgtttgt gaacagacag gccctgagta
     3841 tcgagaaatg actcccaaac catttcgctc agcgtagggt gagggtgaat agtttccgca
     3901 atatcgttgg cggtaagtcc ggccgacatt gccagggcga cttccgagat caagttgctc
     3961 gcgtcatgac cgatcatggt gcaaccaacg actcgccctg tgtcgcgatc cacaacggct
     4021 ttaactagcc cctctgtttc gccaatacac atggcttgag aattttcagc gtagaaaaac
     4081 tttccgatcg cgacattaat tcccttttgg cgcgcctggt cgggttgtag gcccaccgag
     4141 gccatttcgg gaatcgtgaa aatggcgcca gggacggtgt tgtagtcggt cttggcttcg
     4201 ctgccacaca ttgctcccac tgcaattccg gcatcgtacc acgccttgtg ggcaagcatt
     4261 tgaccgccga caacatctcc gccggcatag aggcctctgc agtgagtttc gagacggtcg
     4321 tctactaata tgtgcccgcg gggagtggtt tgcacgccaa tggtttcgag tccgagatcg
     4381 tcggaattcg gtttgcggcc aattgcgagc aacactttat cagtaagcag tgtttctgtt
     4441 ttgttttgta cgtcgagatg cagtttgaca gcctgttcac cgtcttcgcg gacctccacc
     4501 ttcgccactt tagagtgcag gtggatgcga atgccttctt tagaaaagcg ctgcatgagc
     4561 agttgcacaa tttctggatc catcgccgat aacacttggt tcgagtgttc aagcaaatga
     4621 atgcgcacgc cgaatttgct taaaatgctg gcaaattcgc aggccacaac accgccgccc
     4681 acgatggtca gggtgtccgg caactctcgc atgagcacga tgtcgtcggt cgtgaggata
     4741 tgctcgtgat cggcctttaa aacatcggtt tcagaagggc gagacccagt ggcgacgaat
     4801 aaaaaccttg ccgtgagctc agttcgaaaa ccttcgtcgg cagt
//
LOCUS       DAOMXO010000087         4842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9377_length_4842_cov_5.031335, whole genome shotgun sequence.
ACCESSION   DAOMXO010000087 DAOMXO010000000
VERSION     DAOMXO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9377_length_4842_cov_5.031335"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLH57_02880"
     CDS             complement(<1..360)
                     /locus_tag="PLH57_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01583.1"
                     /translation="MNMTTSTTLAAGTGTSFVIRGCQICDNTNLKSAMFLGYLPPVNT
                     MPKIGERPKEEPSYPAELLFCPTCELAQLSLVVDPKILFPPEYPYTSGTTKILRENFA
                     ELYQETNTLFPLKADDLV"
     gene            complement(357..1349)
                     /locus_tag="PLH57_02885"
     CDS             complement(357..1349)
                     /locus_tag="PLH57_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK01584.1"
                     /translation="MSKSKSIFVTGGTGFIGSAIVRRLVREGHRVKVLDDDSRGNVSR
                     LEDIKGQFEFVRGDVRDGASVLAASKGADSVFHLAFVNGTEFFYSKPELVLDVGVKGM
                     VHVLDACIKNNIPELVLASSSEVYQVPPTVPTDESAPLVIPDVHNPRYSYAGGKLISE
                     LMAINYGRKFFERVLIFRPHNVYGPNMGWEHVLPQFIMRFKKLLKEHEASSEPIPFQI
                     QGTGRETRAFNFIDDFVDGIVTMHDKGKHLEIYHIGTEEEISIRDVAKAVGGFFGKEI
                     NIIEGPLQKGGTPRRCPNISKMRALGYAPRFSFREGLPLIARWYDANYKLEGEK"
     gene            complement(1346..2344)
                     /locus_tag="PLH57_02890"
     CDS             complement(1346..2344)
                     /locus_tag="PLH57_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRK01585.1"
                     /translation="MNDRRLRIGVVGRGFGKAVHVPAFAADPRCEVVFNCSNDWKSQF
                     DLHDFDAISIATPADIQPEITLAALERGKHVFCEKPLATDLTTARRLLESAKQAKVAH
                     MIDFEFQEIPAWIEAKRLIRNEELGAIRHVSVQWNIEIYAIRHGLKDSWKIRSEQGGG
                     ALNAFGSHAFYNIEWLLGPITSVAATLHPTSDDEYVSANLTLKHGAPVALQLSTHAFL
                     GNGHRITVYGENGTLVLNNQSNDYIRGFELWSAGRTDSALRQVLVPELDATNPALSVK
                     DGRVWAVHQLARRFIDWALMGAPQTPNFENAYRVQVLIDAAKRSSINRNQEIGIES"
     gene            complement(2344..3576)
                     /locus_tag="PLH57_02895"
     CDS             complement(2344..3576)
                     /locus_tag="PLH57_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRK01586.1"
                     /translation="MTDKNAKSFTTPRDLPTDICVVGGAGHIGLPLAILLASKGKRVV
                     AYDLNTAVLEEVRQGKMPFKEEGAQELLTEVLKTGLLGLSNNPASVQDAKTIIVTIGT
                     PVDEFHNPSLAAIKKCFLDLSPYLSEEQLVVLRSTVSPGVTEWIDSYLKSIGKNLKVA
                     FCPERVVQGLAIKELQQFPQIISATSKEAENEAARLFEELCPEVVRLTPTEAEFAKLF
                     ANAYRYIQFAMANQFYMIANNAGVDYYRVLDGLKKNYPRARDIPKAGFAAGPCLFKDT
                     MQLVAFSNNQFSLGHSAVLINEGLVLYIADQIAAKHPLADMTIGLLGMAFKSETDDVR
                     SSLSYKLKKVLDFKAKSVLCTDPYVETDKTLLPVDEVIRRSDLLVLCTPHEQYKSLDL
                     GDKPLVDVWGFRKTGTLV"
     gene            3844..>4842
                     /locus_tag="PLH57_02900"
     CDS             3844..>4842
                     /locus_tag="PLH57_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01587.1"
                     /translation="MRLQALASKILLLVVGVLFGIQVTFALKYSIDIPCNDTWSLFDP
                     GRIQRAFDLGWLLGFHNEHRIFTTKLAAWILYRINDLNLVHLLAVGHLYFGAAVVVTL
                     LLLRNLIKAPLGWLAVFGTALLMTPLNWENHVVPFHMQFHASMFFSLLYLHLVFSVGD
                     RNHWTWSRVLSAGFCGFLGAFSFAGGVIQMIAAFGVLLITVGSSAGWRAPKVPKTAWG
                     LIVLTFSVIALYFVGYQKPGHHPDPILPLSLQFWRFYAELLSLGFGLREISVERSTMV
                     LFISHAPAVFLLVKTRFLTKANSDERKIIAASAGLVLIIASVASARFGFGLVQAKQV"
BASE COUNT         1164 a         1133 c         1233 g         1312 t
ORIGIN      
        1 aacgaggtcg tcggctttaa gcgggaacaa agtatttgtt tcttggtaaa gttcggcgaa
       61 gttttcacga agaattttag tggtgccgct agtgtaggga tattccggtg ggaacaaaat
      121 ttttggatcg actacgagac tgagctgcgc caactcgcag gtaggacaga acaataattc
      181 tgcagggtaa gaaggttctt cttttgggcg ctcgccgatt ttaggcatgg tgttaactgg
      241 tggcaagtag cctaggaaca tagccgattt taaattcgta ttgtcgcata tttgacaacc
      301 gcgaatgaca aaacttgtac cggtaccagc agcgagagtt gttgaagtag tcatgttcat
      361 ttttcacctt ccagtttgta attcgcgtcg taccagcgag caatcaaagg cagaccctcg
      421 cggaacgaaa aacggggcgc atagcccagc gctcgcattt ttgaaatgtt aggacaacga
      481 cgtggagtgc cgcctttttg aaggggccct tcaataatat taatttcttt gccaaagaaa
      541 ccgcccacgg cctttgccac atcacgaata gaaatctctt cttcagtccc aatatggtaa
      601 atttctaggt gtttgccttt atcgtgcatg gtgacgattc cgtcgacgaa atcatcaata
      661 aaattaaatg ctcgagtttc gcgacctgtt ccttgaattt gaaaggggat cggctcactg
      721 gaagcttcgt gttctttcag gagctttttg aaacgcatga taaattgcgg aagcacgtgt
      781 tcccagccca tgttggggcc gtaaacgttg tggggcctga aaatgagaac tctttcaaaa
      841 aatttacgac cgtaattgat ggccattagt tcgcttatga gtttgccgcc agcgtaagaa
      901 taacgagggt tatgaacatc gggaataacc aacggcgcgg actcgtcggt cggtacggtg
      961 ggtggaactt gatagacttc agaactagag gccaaaacta gctcgggaat gttattttta
     1021 atacaagcgt caagcacatg caccatgcct tttacgccta cgtcgagaac gagttccggt
     1081 ttggaataaa aaaactcggt gccattcacg aaggctagat gaaatacgct gtctgcacct
     1141 ttcgaagcag ctagcaccga agcgccatct cgcacgtcgc cacgcacaaa ttcgaactgg
     1201 cccttaatgt cttcaagtcg cgaaacattg ccccgcgaat cgtcgtcgag gactttaact
     1261 ctgtgacctt cgcgcaccag acgacgcacg atagccgaac caataaaacc ggttccccca
     1321 gtaacaaaaa tggacttgga tttactcatg attcaatgcc tatttcttgg tttctattga
     1381 tactcgatct ttttgcagca tcaatcaaaa cctgcacgcg atacgcattt tcgaagttcg
     1441 gtgtttgggg cgctcccatc agcgcccagt cgataaagcg tctcgctaac tgatgaacag
     1501 cccatactcg gccatcctta actgaaaggg cggggttggt tgcgtcaagt tctggtacca
     1561 aaacctgacg cagggcggag tcggttctgc cggccgacca cagttcaaat ccccgaatgt
     1621 aatcatttga ttggttattt agaacaaggg tgccgttttc cccgtaaaca gtgattcggt
     1681 ggccattgcc taaaaatgcg tgggtgctaa gttggagagc aacgggcgcc ccgtgtttca
     1741 gggtaagatt ggcggaaacg tattcgtcgt ctgacgtagg gtgaagtgtc gctgctacac
     1801 tcgtaatcgg acctaagagc cactcaatgt tatagaaggc gtgcgatcca aaggcattca
     1861 gcgcaccgcc gccctgttcg gaccgaatct tccagctgtc ttttaggccg tgtcgaatag
     1921 cgtagatctc tatattccac tgaacactta cgtggcgaat ggcaccgagc tcctcattgc
     1981 gaatgagacg cttagcttcg atccaggctg gaatttcctg aaattcgaaa tcgatcatat
     2041 gggcaacctt cgcctgtttt gcagactcga gcaaacgccg ggccgtcgtt agatcggtag
     2101 ctagcggttt ttcgcaaaaa acgtgtttgc cgcgttccag ggcggccaaa gtgatttctg
     2161 gttgaatatc tgccggagtt gcaatgctaa tggcatcgaa gtcatgaaga tcgaactgag
     2221 atttccaatc gttcgaacag ttgaatacga cttcacaacg ggggtctgct gcaaacgctg
     2281 gtacgtgtac agctttgccg aaaccacgac ctactactcc gatacgaagc ctgcgatcgt
     2341 tcatcaaacc agagttccag ttttacgaaa tccccaaacg tcgaccaggg gtttatcgcc
     2401 tagatcaagc gacttgtact gctcatgtgg tgtgcataaa acgagcaagt ctgagcgacg
     2461 aatgacttcg tcaaccggca agagagtttt gtcagtttcg acatatgggt cggtacagag
     2521 tacgctctta gctttgaaat ccaaaacttt cttgagctta taggatagcg aagaccgaac
     2581 gtcgtctgtt tctgatttaa atgccattcc gagtagtcca atcgtcatgt cggcgagagg
     2641 gtgcttagcg gcgatttggt cggcaatgta aaggacgagt ccttcgttta ttagaaccgc
     2701 agaatgacca aggctgaatt gattgttcga aaatgccacg agctgcatgg tgtctttaaa
     2761 tagacacgga ccagcggcaa aacctgcttt cggaatgtcg cgcgcacgtg ggtagttttt
     2821 ctttaacccg tcgaggactc ggtaatagtc cacacctgcg ttgtttgcga tcatgtaaaa
     2881 ctggttggcc atggcgaatt gtatgtaacg ataggcattc gcaaataact tcgcgaactc
     2941 tgcttcggtc ggagttaggc gaacaacctc aggacaaagt tcttcgaaaa gtcgtgcggc
     3001 ttcgttttcc gcctctttcg aagttgcact aataatctga ggaaactgtt gcagttcttt
     3061 tatcgcgaga ccctgcacca ctcgttcagg gcaaaaggcc accttcaaat ttttgccaat
     3121 agattttagg tagctgtcga tccactctgt cactcccggt gacaccgtgg aacgaagtac
     3181 cacgagttgt tcctctgaaa ggtaagggga aagatctaag aaacattttt ttatcgcagc
     3241 aagcgaaggg ttgtgaaatt cgtcgaccgg cgtgccgatc gtaacaataa tagttttggc
     3301 atcctgaacc gaagctggat tatttgaaag acccaaaagt ccggttttta aaacctcagt
     3361 caacagttct tgagcgccct cttctttgaa gggcattttt ccttgccgta cttcttcgag
     3421 cactgcggtg ttaaggtcgt aggcaacaac gcgttttcct ttactagcca acaagatcgc
     3481 aagcggcaga ccaatgtgac cagcgccacc gactacacag atgtcggttg gtagatcgcg
     3541 aggcgtggta aaagatttag cgtttttgtc cgtcatatag ttgggctcac aatgtgggtt
     3601 gaaaatcgcc tggaacataa caagcgaggg cgagcaattc aaggattgct gtgcagtcgg
     3661 ttgttcctca gggtgcggcg ccaactttat gggtgagcgg ccggagtcta tagccaaatt
     3721 tgcgaccacc aggcgtcttg cctttgagtc gctcagggac gccccgtcac cccgtaaagt
     3781 tggctccgat ctccagagcg gttgtcgctt gccaaagaat cgcctttcgt acatgaccaa
     3841 aagatgcggt tacaagcgtt ggcgtcaaaa atacttcttt tggtcgtagg agtgctgttt
     3901 ggcattcaag taacctttgc tctcaagtat tcgatcgata taccctgtaa tgacacatgg
     3961 agtcttttcg accctggccg cattcagagg gctttcgacc taggctggtt gcttggcttt
     4021 cacaatgaac atcgcatttt tactaccaag ttggcggctt ggatcttgta ccgaatcaac
     4081 gatttaaacc tggtgcacct attggccgtg gggcacctgt attttggggc agctgtggtc
     4141 gtgacgttgc tgcttctccg taacttgatc aaggcacctt tgggttggct cgcagtgttc
     4201 ggcacagctc tgctcatgac cccattgaac tgggaaaatc atgttgtgcc gtttcatatg
     4261 cagtttcatg cctcgatgtt tttttcgctt ttgtatctgc atttggtttt tagcgttgga
     4321 gaccgaaacc actggacttg gtcgcgcgtt ttgtcggccg gcttttgcgg gttccttggc
     4381 gcgttttcgt ttgcaggcgg cgtgattcag atgatcgcag ctttcggagt tttgctcatt
     4441 accgtaggga gcagtgctgg gtggcgggcg cctaaagtgc cgaaaaccgc atggggattg
     4501 atcgtactta ctttttcggt gatcgcgctt tatttcgtag gctatcaaaa acccggacat
     4561 cacccggacc cgattctccc tttgagttta cagttttggc ggttttacgc agaacttttg
     4621 agtttaggtt tcgggctacg cgagatttcg gtcgaacgat cgacgatggt tctttttatt
     4681 tcccatgcgc cggctgtttt tctgttagtg aagacgcgtt tcttaactaa agcaaattca
     4741 gatgaacgaa aaataattgc cgcctcggcc ggattggtgc tgattattgc gagtgtcgcc
     4801 agcgcgcgct ttggctttgg gctcgtgcag gccaaacaag tg
//
LOCUS       DAOMXO010000088         4837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9389_length_4837_cov_5.124216, whole genome shotgun sequence.
ACCESSION   DAOMXO010000088 DAOMXO010000000
VERSION     DAOMXO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9389_length_4837_cov_5.124216"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLH57_02905"
     CDS             complement(<1..687)
                     /locus_tag="PLH57_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRK01588.1"
                     /translation="MNYENFIIERIGEVVVLRINRPKALNALNRALLLEWEKILADTE
                     LRASKVLVITGSGEKAFIAGADISEMHGMSGQNAAEFGKLGQRVSMMLEEFPCPVIAA
                     VNGFALGGGCEMAISCDMVVASDTAVFGLPEVSLGLIPGFGGTVRLARWVGLAKAKEL
                     IFTGRKITAAEALSIGLTNKVVAREKLIDEAVVLGNEIIRNSVTAVRSAKASIRLSGE
                     SATAKDGLDIE"
     gene            complement(692..1558)
                     /locus_tag="PLH57_02910"
     CDS             complement(692..1558)
                     /locus_tag="PLH57_02910"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRK01589.1"
                     /translation="MTTKSKIQVIGVVGAGQMGSGIAQVGAASGLGIVLQDVANESLT
                     RARARIENSLGKLVEKSKISAEEAKAALGRIQFTQHLEELSRADLVVEAVVEKPELKF
                     EIFRKVDSICKEEAILASNTSSISITAIAAHTRRPDRVIGMHFMNPVPIMKLVEVIRG
                     LRTSDATFSSVKQVGEQMGKTCVESKDFPGFVVNRILMPMINEACYALYEGIAKAEDI
                     DNAMKLGTNQPMGPLALADFIGLDTCLAILNVLHEGLGDPKYRPCPLLKQYVSAGLVG
                     KKVGHGFYKYES"
     gene            complement(1595..2989)
                     /gene="mnmE"
                     /locus_tag="PLH57_02915"
     CDS             complement(1595..2989)
                     /gene="mnmE"
                     /locus_tag="PLH57_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRK01590.1"
                     /translation="MAGSYFSDDTIIAVATSLAADAGVGIIRVSGKNAKAVALKLDPN
                     FKSEPARYLHRIQICKMATGEVLDDGLSVYFRAGASFTGEEVVELQLHGGRHVLQAVL
                     QEALSTGLCRLALPGEFSFQAVRNGKLSLSGASALQKMISARSDLEVKVARNTLSNDR
                     MADIRKMGDIVRDLLTKVELSIDFVDQDVEVTTSEFILDKANEVITSCDRIIKKVNSA
                     RRVAQGFEVVLLGEPNAGKSTLFNAMLAEDRAIVSPIAGTTRDIITEELLVGPYRLRL
                     ADTAGIRGTSEVIEATGVERALMLASAAEIVLHIYDGTSVSVDALKELCVLPKKNNSI
                     VVVTKADLMTPENTARIRSELVGIEPVVFVSAKSGTGLLELFEAIRTVLDANHGDAKS
                     GFLATEYQLDMLVRFQKAVLEACTLVKTRGLRDPELVSAELRIAAQALENVIGETTPD
                     TVLTKIFSEFCIGK"
     gene            complement(3011..4588)
                     /gene="yidC"
                     /locus_tag="PLH57_02920"
     CDS             complement(3011..4588)
                     /gene="yidC"
                     /locus_tag="PLH57_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRK01591.1"
                     /translation="MENKRMFLVVALCAGIFLLWQQLFVKPHVGAPLQNVVSTATNEN
                     VKGVASNTTSTTPNSTISEEKVVTLGGAGMKVKIATRGGVITQIESNRGETVRAVDLN
                     SLIGGKKELEILSPSQEWMYLASLPFKVQSSEKSGDDTRKIVLSYSDEKVAVTRTFVL
                     NPDAHTVDNDTTFQFRNTYPNYLFVNISAARDVAKEYSENERREAVFVNDDGLSTWAL
                     SDMSEVKEDLAGGYWAGISSRYFLKAILDNGSATKPQFQARPTDSGEVAASLVYRLTG
                     PSLNLPVRYYYGVKEIDSLKKVGNKLDLAVDFGWFTVIAYPMLQALKWLHTIFGNFGV
                     AIILLTIAIKMILYPLTYKSMKGMKEMQRIQPQIKRLQEKYKDDRQRMNQETLKLMQS
                     NGYNPMSGCMPMLLQMPVFIALYNVLYGAFDLYRQPFFGWITDLAARDPYFVTPIVLT
                     LLMFLQQKITPTAAPMDPAQQKMMQLMPLIFGAMMAFLPAGLTLYMLVNSVVSIAQQV
                     VILRILNAKEQTPVVVS"
     gene            complement(4578..>4837)
                     /gene="yidD"
                     /locus_tag="PLH57_02925"
     CDS             complement(4578..>4837)
                     /gene="yidD"
                     /locus_tag="PLH57_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRK01592.1"
                     /translation="RSMKDIVSLPVLVLIRFYQVVFSPFFSVIFGANCRYEVTCSQFA
                     EKAVRRNGAIRALPQILLRLLSCNPWFTFRGEVKREGIYGK"
BASE COUNT         1270 a         1271 c          954 g         1342 t
ORIGIN      
        1 ctcaatgtct aacccatctt ttgccgtcgc gctctcgccc gacaacctaa tgctcgcctt
       61 agccgacctt accgctgtga ctgagttcct aataatttca tttcctaaca ccacggcttc
      121 atcaattaat ttttctctgg ccaccacttt attagtcaaa cctatcgaca aggcttcagc
      181 cgctgtaatt tttcttccgg taaaaattag ttccttcgct ttagcgagtc caacccatct
      241 cgctaatcgc actgttccac caaaaccggg aattaaaccg aggctcactt ctggcaatcc
      301 aaatactgcg gtatccgacg caactaccat gtcgcagcta atcgccattt cgcacccgcc
      361 ccctagtgca aagccattta ccgcggcaat gactggacag ggaaactcct ctagcatcat
      421 ggaaactcgt tgacctagtt ttccgaattc cgcggcgttt tgcccggaca ttccatgcat
      481 ttctgagata tccgcacccg caatgaaagc cttttcgccg gatccagtga ttactaggac
      541 tttcgaagcc cgcagttccg tatctgccag aattttttcc cactctaata gcagcgcgcg
      601 atttaatgca ttaagtgctt ttggccgatt aattcttaga acaacaactt cgccaattcg
      661 ttcaataata aagttttcgt agttcaaaca atcagctttc gtatttatag aagccgtggc
      721 caactttttt acctactagc cccgccgaca catattgttt caaaagtggg cagggtcgat
      781 acttagggtc gccgagccct tcgtgtaata cattgagaat cgcgagacag gtatcgagtc
      841 caataaaatc agccaatgca agcggaccca taggctgatt ggtgccaagc ttcatcgcat
      901 tatcgatgtc ttcggctttt gcgatacctt cgtaaagcgc ataacaagct tcgttaatca
      961 tcggcattaa tattctgtta acaacgaaac ctggaaaatc tttagattct acacaagttt
     1021 ttcccatttg ttccccgact tgtttgacac ttgaaaatgt cgcgtcggac gtacgaagcc
     1081 cgcgaataac ctctacaagc ttcataattg gaacgggatt cataaaatgc atacctatca
     1141 ctcgatctgg gcgccgtgta tgcgcggcaa ttgctgtaat cgaaatagac gaggtattag
     1201 acgctaagat cgcctcttct ttacagattg aatcgacttt tctaaatatt tcgaacttca
     1261 actctggttt ttcaacaaca gcctcaacca ctaagtcagc ccgcgaaagc tcttctaaat
     1321 gctgtgtaaa ctgaatgcgc cccagggccg ccttagcctc ttcggcgcta atcttcgatt
     1381 tttcgactag ttttccaaga ctgttttcaa ttcgagctcg agctctagtt agggactcat
     1441 ttgcaacgtc ctgcagaacg atacctaagc cacttgcagc accaacttgt gcaatacccg
     1501 aacccatttg acccgcgcca acaacgccga tcacttgtat tttagatttt gttgtcatgg
     1561 gcgaactgta gcgccgggag cgcggcattt caacctattt gccgatgcaa aactcactga
     1621 aaatctttgt caaaaccgta tcgggagtgg tctcgccgat cacgttttcc aatgcctgcg
     1681 cggcaatgcg taattcggca cttacaagtt ctggatccct aaggcctcga gtttttacga
     1741 gtgtgcaagc ttccaataca gctttctgaa accgcacaag catatctaat tgatactctg
     1801 tggctaggaa gcccgacttc gcatctccat gattcgcatc taacaccgtg cgaatagctt
     1861 caaacaactc taaaagaccc gtaccgcttt tcgcgctgac aaatactacc ggctcaatac
     1921 ccaccaattc acttctaatt cgggcggtgt tctcgggcgt cattaaatcg gccttggtaa
     1981 caacgacaat ggagttattt ttcttgggaa gaacacaaag ttcttttagg gcatccaccg
     2041 acacacttgt tccgtcgtaa atatgcagca ctatttccgc agcacttgcc agcattagcg
     2101 ctcgctcaac acctgtagct tcaattacct cgctagtacc tcggataccc gcagtgtctg
     2161 ccaacctcag ccggtagggc ccaactagca gttcttcagt aataatgtct cgagtagttc
     2221 ctgcaatggg cgaaacaatc gctcgatcct ctgcaagcat tgcattaaac aaagtagatt
     2281 tccccgcatt tggttcccca agaagcacaa cttcaaatcc ttgcgccacc cttctcgcgg
     2341 agttcacctt ttttatgatc cggtcgcagc tagtaattac ttcgttagcc ttatctagaa
     2401 taaactccga agtggtcact tcgacatcct gatctacaaa atcaattgag agttccactt
     2461 tcgttaacaa atccctaacg atgtctccca tttttcgaat gtctgccatt cggtcattcg
     2521 aaagcgtgtt gcgagcaact ttaacttcca aatcggacct ggcgcttatc atcttttgca
     2581 aagcgctggc gccgcttaag gaaagtttgc cgtttctaac tgcctgaaag gaaaactctc
     2641 ccggcagggc taatcgacat aaccctgttg aaagcgcttc ctgcaagacg gcctgaagca
     2701 cgtgtcgacc gccatggagt tgcagctcca caacctcttc cccagtaaat gaagccccag
     2761 ctcgaaaata aacggaaagt ccgtcgtcaa gcacttctcc agttgccatt ttacaaattt
     2821 gaatgcggtg caaatagcgc gcgggttctg atttaaaatt tggatccaac tttaaagcga
     2881 ccgctttggc atttttaccg gaaacacgaa taatccccac tccggcgtca gcggccagac
     2941 tcgttgcaac tgcaattatg gtatcatccg aaaaatagct tcctgccata gttacttctt
     3001 aaatttcgta ctacgaaacc acgaccggcg tttgttcctt cgcgttaagg attcgtagta
     3061 taaccacctg ttgagcaata cttacgacac tgttaaccaa catatacagc gtaagtccgg
     3121 cgggtaaaaa cgccatcata gccccgaaaa tcaacggcat aagttgcatc attttttgtt
     3181 gggctgggtc catgggtgca gctgtcggtg taattttttg ttgcaaaaac attaacaaag
     3241 ttaacacgat cggagtcaca aagtatggat cgcgggcagc caaatctgtg atccaaccaa
     3301 agaatggctg gcgatataaa tcgaacgctc cataaagaac attgtagagc gctatgaata
     3361 ccggcatttg taacaacatc ggcatacagc cactcattgg gttgtaccca ttagactgca
     3421 tcagcttcag ggtttcctga ttcattcgct gccgatcgtc tttgtatttt tcttggaggc
     3481 gtttgatttg cggttgaatt cgctgcatct ctttcatgcc cttcatggac ttgtaagtca
     3541 gaggatacaa aatcattttt atagctatag ttaacaaaat gatcgcaaca ccgaagtttc
     3601 caaaaatcgt gtgcaaccat ttcagggcct gaagcattgg gtatgcaatt acggtaaacc
     3661 aaccaaagtc cactgcaaga tccaacttgt tccctacttt tttcagggaa tcgatttctt
     3721 ttacaccgta ataataccgt actggtaaat ttaaactggg gcccgtcaag cgatatacca
     3781 agctagccgc cacttcgccg ctatctgtgg gtcgtgcctg aaactgtggt tttgtcgccg
     3841 atccgttgtc gagtattgcc tttagaaaat aacgcgaaga aattccggcc caatatccgc
     3901 ctgccaaatc ttccttaact tcgctcatgt ctgaaagcgc ccacgtcgac aggccatcat
     3961 catttacgaa tacggcttct cttcgttcat tttcgctgta ttcttttgcc acgtctcgcg
     4021 cggcactaat atttacaaaa agataattgg gataggtatt tcgaaactga aaagtagtgt
     4081 cgttatcaac ggtgtgtgcg tcgggattaa gcacgaatgt tcgcgttact gctacttttt
     4141 cgtcggaata actcagaact atttttcggg tatcgtcgcc gcttttttcc gacgattgta
     4201 ccttaaaagg cagtgaagca agatacatcc attcctgcga cggactcaaa atttcgagtt
     4261 ctttctttcc tccaattaac gagttcaaat caactgcacg cactgtctcg ccgcgattcg
     4321 attcaatttg tgtaatgact ccgccgcgag tggcaatttt tactttcatt cccgcgccgc
     4381 caagggtgac gaccttttct tcacttattg tcgaattagg cgtcgtactt gtggtattgc
     4441 tcgcaactcc ctttacattt tcattagtag ctgtcgaaac cacattctgt agtggtgcac
     4501 caacgtgcgg ctttacgaaa agttgctgcc aaagcagaaa aattcctgcg cacaaagcga
     4561 caaccaaaaa cattctctta ttttccataa attccctctc gcttgacttc gccccgaaac
     4621 gtaaaccagg ggttgcagga caataacctc aacaaaatct gtggcaacgc gcggatcgcg
     4681 ccatttcttc gtacggcttt ttccgcaaac tgactgcacg ttacttcgta tcgacaatta
     4741 gctccaaaaa tgaccgaaaa aaaaggcgaa aacaccactt ggtagaatcg aattagaact
     4801 aaaacgggca aagacactat atctttcata ctccgcc
//
LOCUS       DAOMXO010000089         4828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9405_length_4828_cov_5.232977, whole genome shotgun sequence.
ACCESSION   DAOMXO010000089 DAOMXO010000000
VERSION     DAOMXO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9405_length_4828_cov_5.232977"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PLH57_02930"
     CDS             <1..743
                     /locus_tag="PLH57_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK01593.1"
                     /translation="VTQVGDYIFRVCFIDPFQGSVMAKFAHGTLKVKKAAILRDVKND
                     YSVGLANFFTETFKKLGGEVVVDQSYSAGDVDFKSQLTSIKTKEPEAIFVPGYYTEVG
                     LIARQARELGIKAPLLGGDGWDSAKLTEIGGKALEGSYFSNHYSPDDTSEHVQKFIAA
                     YKAKFGGVPDGLAAMGYDAAMVLADAMKRAKTLEKDDIREALANTKDFQGVTGRITID
                     HDRNAVKPAVVLEVKSGGFAYKETINPS"
     gene            743..1645
                     /locus_tag="PLH57_02935"
     CDS             743..1645
                     /locus_tag="PLH57_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRK01594.1"
                     /translation="MTEFLQHIINGLGLGAIYALIALGYTMVFGILQLINFAHGDVYM
                     LGAFIGYYAGRYLGLANVPGLGSLGAALALAMLGAAVAGYLIERFAYRPLRKSPRINV
                     LITAVGVSLLLEFGGQIIFGADPKFFPTLYVPGEPFMIGTLQMNRLQLLVLAISLVLM
                     TGLRTIIFKTKTGRAMRAVSFNHDNAALMGIPVDRIISVTFMLGSALAGAAGILVGLT
                     YPKIDPLMGVMFGLKAFVAAVVGGIGNVTGAVIGALLMGVAESLVIAYWASTYRDALA
                     FGILILILLFKPAGLLGTHRQEKV"
     gene            1660..2658
                     /locus_tag="PLH57_02940"
     CDS             1660..2658
                     /locus_tag="PLH57_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRK01595.1"
                     /translation="MNWKNNSTLRAGVVMILGILFFFGLGAIIPTLFNAYVFTIVMYA
                     GINVTAAVSLNLVTGITGQFSMGHAGFMSVGAYAAAFLTTVILPTAEIPPALVPLVFA
                     GSLFFGGIVAAILGLVVGLPSLRLRGDYLAIVTLGFGEIIRVVILNVETIGGARGLPG
                     IPQWTSFGWVYSVAFLAVLTVRRLLASSHGRAFLAVREDEIAAEAMGVNTTRYKVLAF
                     IIGAFFAGVSGGLFGHFLTYLNPATFDFNRSFEIIIMIVLGGLGSTTGAVAAALFLTF
                     LREALRPLQEITQLDFRMIIYSGLLIVLMLTRPNGFLGTKELTDFGFFRRFKGLRQ"
     gene            2655..3437
                     /locus_tag="PLH57_02945"
     CDS             2655..3437
                     /locus_tag="PLH57_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK01596.1"
                     /translation="MTGLQAKNLTMQFGGIKAVTDLSFELPTGSITALIGPNGAGKTT
                     VFNMLTGVYHPTKGSVEFRKEQISGLRPFEIAKKGVARTFQNIRLFKNLSVLDNVRVG
                     LDIHSQFGLVHAFLQSSRLVESEKTRVDDALKLLAIFDLQSRAQDLAKNLPYGAQRRL
                     EIARALATGAQVLLLDEPAAGMNNQETQELMHTIERIRKDFGLTILLIEHDMKLVMGI
                     SQKVIVLDYGVKIAEGLPKEVQKDPKVIEAYLGKAAAKEHTP"
     gene            3434..4183
                     /locus_tag="PLH57_02950"
     CDS             3434..4183
                     /locus_tag="PLH57_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009728584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK01597.1"
                     /translation="MNSGTSNLSALSQPILEFRNVNVHYGAIHALKGVSLKVETGKIV
                     TLVGANGAGKSTTLRAISGLLKPSSGDVLFRGQSIAGQRTDRIVALGISQAPEGRGIF
                     ANMTVAENLELGAYLRKDKEEIENDRAHVFELFPRVKERLWQSAGTLSGGEQQMLAIG
                     RALMARPSLLLLDEPSLGLAPQIIERIFEIIVKVNRSGTTVLLVEQNALLAMEIAHYA
                     LVLETGKVVAEGPSVELSRSPEIRKAYLGED"
     gene            4207..>4828
                     /locus_tag="PLH57_02955"
     CDS             4207..>4828
                     /locus_tag="PLH57_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01598.1"
                     /translation="MNRRTVLWALITAFVLAGGAPAVLAQEAELSEPDYPALKKELPS
                     PTKSIETYEDARNERIKQTGIPDWAVSAGTELAPFGKADFRIPNPTASSSRFRDGNPE
                     MTGILVSLERFLIRDYGMLSVGVDGGAYVSKAKEPFDKMKPAIWSFAPTAHYELVFMH
                     RQILVPSVRFNYQFLRYSYTYHSQTVKGIKRYPRIDIGMLLYLNFLE"
BASE COUNT         1125 a         1192 c         1244 g         1267 t
ORIGIN      
        1 aagtcactca agtgggagac tacatttttc gggtttgctt catcgaccct tttcaaggtt
       61 ccgtcatggc gaaattcgca cacggaactt taaaggttaa gaaggctgcc attcttcgcg
      121 acgttaaaaa tgattactcc gtcggattgg cgaacttttt cactgaaacc ttcaagaaat
      181 tgggtggaga agtggtggtc gatcaaagtt actcggcagg tgacgtagat tttaaaagtc
      241 agctgacatc tataaagacc aaggaaccag aggccatttt tgttcccgga tactacaccg
      301 aagtagggtt gatcgcgcgc caagcgcgtg agcttggaat taaagctccg ctgctcggtg
      361 gcgatggctg ggactccgcc aaacttactg aaatcggtgg caaagctttg gaaggcagct
      421 acttttcgaa tcactactca ccagatgaca cgagcgaaca cgtacaaaag tttatcgccg
      481 cttacaaagc caaattcggt ggcgtgcccg acgggttagc tgccatgggc tacgatgccg
      541 ccatggtcct tgccgatgcc atgaagcgcg ccaaaacact tgaaaaagat gacattcgcg
      601 aagcgcttgc caacaccaaa gattttcagg gtgtcacggg gcgtatcacg attgatcacg
      661 accgcaacgc agtgaaaccc gctgtcgtgc tcgaagtaaa atctggcggc ttcgcctata
      721 aagaaacgat taatcccagc taatgactga atttcttcag catatcatca atggtctagg
      781 cctcggtgcg atctatgctc tgatcgcgct tggttacacc atggtgtttg gaatattgca
      841 gctcatcaac ttcgcgcatg gcgacgttta tatgttgggt gcttttattg ggtattacgc
      901 gggtcggtat ttaggactgg cgaatgtgcc cggtttagga tcgctcggtg ctgcgctcgc
      961 actcgccatg ctcggcgccg cggtcgcagg atatttaatc gaacgtttcg cctatcgacc
     1021 actgcgtaaa tcccctcgca taaatgtttt aatcaccgcg gtaggtgtgt cgctacttct
     1081 agagtttggc ggccaaataa tttttggcgc ggatccaaaa tttttcccca ctctctacgt
     1141 gccgggcgaa ccttttatga ttggaactct gcagatgaac cgtctgcaac tattggttct
     1201 cgccatcagt ttggtgctca tgaccgggtt gcgtacgatt atctttaaga ccaaaaccgg
     1261 acgagcgatg cgagccgtct cgtttaatca cgacaatgcc gctctcatgg gtattccggt
     1321 ggatcgcatt ataagtgtga ccttcatgct agggtccgcg cttgccggtg cggctgggat
     1381 tctcgtaggg ctgacctatc caaaaattga tccgctcatg ggtgtgatgt tcggcctcaa
     1441 agcctttgta gccgcagtgg tcggaggtat aggcaacgtc acgggtgcag tgattggagc
     1501 gttgttgatg ggcgtagccg agagtctggt gatcgcctac tgggccagta cttatcgtga
     1561 tgcgctcgcg ttcggcatac ttattctcat tttgttgttt aaacccgccg gccttctggg
     1621 cactcatcgg caggaaaagg tgtagaggaa cttcgtcgcg tgaactggaa aaacaactca
     1681 acactccgcg ctggcgttgt catgattctt ggcattctat ttttctttgg gttgggcgct
     1741 attataccga cactatttaa tgcctacgtt ttcacaattg taatgtacgc cggcatcaat
     1801 gttacggccg cggtgtcgct caatttggtt accggaatta ccggccaatt ttcgatgggg
     1861 cacgcggggt tcatgtcggt cggtgcttat gccgcggctt ttttaacgac cgtaatattg
     1921 cctaccgctg aaattccgcc cgccctggtt cctttggttt ttgctggaag tctgtttttc
     1981 gggggcatcg tggctgcgat tttaggactc gtcgtaggtc tgccttcatt gcgccttcgc
     2041 ggcgactatt tagccatcgt cacgttaggc tttggcgaaa tcattcgagt ggtcatactg
     2101 aatgttgaaa ccataggcgg cgccagagga ctacccggaa ttccgcagtg gaccagcttc
     2161 ggctgggtgt actcggtcgc atttcttgcg gtccttaccg tacgacgact tttagcatcg
     2221 tcccacggcc gtgctttttt agctgtgcga gaagacgaaa tcgcagctga agcaatgggc
     2281 gtgaacacca cccgctataa ggtgctcgct ttcattatag gtgcattctt tgcgggtgtt
     2341 tccggcgggc tatttggcca ctttttgacc tatttgaatc cggccacctt tgattttaat
     2401 cgcagtttcg aaatcatcat catgatcgtg ctcggcggtt tgggttcgac taccggtgcg
     2461 gtcgccgctg ctctgttttt gacatttttg cgtgaggcct tgcgtccact tcaggaaatc
     2521 actcaactcg actttcgcat gatcatttat tcgggtttat tgatcgtcct catgctcacg
     2581 cgtccgaacg ggtttttggg taccaaagag cttactgatt ttggtttttt tcgtcgcttt
     2641 aagggacttc gccaatgaca ggacttcagg caaaaaacct tacaatgcaa tttggcggta
     2701 ttaaagccgt taccgacctc tcgtttgaat tacctacggg gagtatcacg gcattgatcg
     2761 gtcctaatgg cgctggaaaa accacggtgt tcaatatgct caccggcgtt tatcacccta
     2821 caaaaggcag cgttgagttc cgtaaagagc agatctcggg attgcgccca tttgaaatag
     2881 ctaaaaaagg ggtagcgcgg acgtttcaaa acattcgtct attcaagaat ctttcggtgc
     2941 tcgacaacgt acgagtgggg cttgatatcc attctcaatt tggattagtt cacgcgttct
     3001 tacagtcttc tcgattggtc gagtcggaaa aaacacgagt cgatgatgcc ttgaaactgt
     3061 tggcgatttt tgacctacag tctcgggcgc aggacctcgc caagaacctg ccctacggcg
     3121 cacaacgtcg tttggaaatt gctcgcgcgc tagcgactgg cgctcaagtg ttgttactag
     3181 acgaacctgc cgccggcatg aacaatcaag aaacccaaga actcatgcac acgattgagc
     3241 gaatccggaa ggacttcggc cttacgatct tgttgattga acatgacatg aaacttgtta
     3301 tgggaattag tcaaaaagtg atcgtgctcg actacggcgt taaaatagcc gaaggacttc
     3361 caaaagaggt ccaaaaagat ccaaaagtaa ttgaagccta ccttggtaag gccgcagcga
     3421 aggagcacac tccatgaata gcggcacctc taacctttca gcgttgagcc agccgattct
     3481 agagtttcgc aatgttaacg tgcactacgg tgcaatccat gcgctcaaag gtgtttcgct
     3541 aaaggttgaa accggtaaga ttgtcacgct cgttggggcc aacggcgccg ggaaaagcac
     3601 cacgctgcgg gcgatttcgg gattgttaaa accatcttct ggtgacgttt tatttcgagg
     3661 tcagtcgatc gccggccaac ggaccgatcg tatcgtggcc cttggaatta gtcaggcccc
     3721 ggaaggccgg ggcattttcg ccaacatgac ggtagccgag aatcttgagc tgggcgcgta
     3781 tttaagaaaa gacaaagaag aaattgaaaa cgaccgggct catgtcttcg aactgttccc
     3841 tagagttaaa gagcggttgt ggcagagcgc agggactctt tccggtggtg aacaacaaat
     3901 gcttgccatt gggcgagcac tcatggctcg gcctagttta cttttgctcg acgaaccctc
     3961 gctggggctt gcgccccaaa tcattgagcg cattttcgag atcattgtta aagtaaaccg
     4021 cagtgggacg acggtcctat tggtcgaaca aaacgccttg ctcgcaatgg aaattgccca
     4081 ttatgccctc gtacttgaga ccggcaaagt cgtggccgag ggccccagtg tcgaactcag
     4141 tcgttctccc gaaattcgaa aagcctacct tggcgaggat taaggttcga gctacactga
     4201 aactcaatga atcgacgcac ggttttgtgg gcactgatta cagctttcgt actcgcgggt
     4261 ggcgcgccag cggttctcgc gcaagaggcc gaactctcgg aaccagatta tccggcgctt
     4321 aaaaaagagc tcccatcgcc caccaaatcg attgaaacct acgaagacgc tcgaaacgaa
     4381 cgcatcaaac aaaccggaat acctgattgg gcggtttccg ctggtacgga gctcgcgcct
     4441 tttggcaaag cggattttcg cattccgaac ccaactgcga gcagctcccg ttttcgcgat
     4501 ggcaatcctg aaatgaccgg aattctagtt tctctagaac gttttctgat tcgcgattac
     4561 ggcatgctct cggtaggagt cgacggtggc gcctacgtta gtaaagccaa agagcccttc
     4621 gacaagatga agcctgcgat atggtctttc gcaccgacag cgcactacga actagtgttc
     4681 atgcatcgac aaattctagt acccagcgtt cggtttaatt atcaattttt gcgctacagc
     4741 tacacctacc attcacaaac cgtgaaaggc attaaacgtt acccgcgcat tgatattggt
     4801 atgctgttgt acttaaattt tctggaac
//
LOCUS       DAOMXO010000090         4785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9507_length_4785_cov_4.593869, whole genome shotgun sequence.
ACCESSION   DAOMXO010000090 DAOMXO010000000
VERSION     DAOMXO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9507_length_4785_cov_4.593869"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLH57_02960"
     CDS             <1..304
                     /locus_tag="PLH57_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01599.1"
                     /translation="FNRSPVPNDAEVLLKTLLAIKYDLDASIINQHIASALPSTGGRL
                     DSAIQVVAMALANQQQKSAPLDRHELRELPPASVQSTSAFDLILLKPIYQGFEGEN"
     gene            349..855
                     /gene="greA"
                     /locus_tag="PLH57_02965"
     CDS             349..855
                     /gene="greA"
                     /locus_tag="PLH57_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRK01600.1"
                     /translation="MSENDSVKPVLSSAVPMTIEGKRMLEDELKRLRSVERPSVIKAI
                     EEARALGDLSENAEYSAAKERQSFIEGRIQELNAKLASAQVIDPTTISTDKVVFGATV
                     VLSDQDSGKEVTYKIVGTDESDIKQNKLSVSAPLARALIGKKVGDEVTVNAPKGSLLY
                     DVVEIRYE"
     gene            803..2998
                     /gene="recG"
                     /locus_tag="PLH57_02970"
     CDS             803..2998
                     /gene="recG"
                     /locus_tag="PLH57_02970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRK01601.1"
                     /translation="MLQKDRFSTTSSKSGTSKSAAAGLHLRSPVSALKGVGPKIAHSL
                     EAKGIRTIEDLVLYLPRDYQDRSQLKPISQLIAGERALVRGTIRKHRFVYLRGNSRGF
                     TQPLLEAQLDDGSGRVIMLKWFRYNRKFFETKFAEQPSILVYGQIRSFGHNFEIIHPD
                     IDWAGDDSGANAERIVPVYSEIEGVHQKLLRRIIASAVAIGVPKLEEEIPQSILTTQD
                     LLPLKDAVLFLHHPPKNFPTDQLKTFASPAQRRLVFEELFKFEWIIGQKRMNIRKEPT
                     VGFPTNPALGLLGEMERQIPFQLTGDQKVAISKIYDDLAQKKPMNRLLQGDVGCGKTL
                     VALASALPPLAAGAQTVIMAPTEILAEQHFKNALNTVGPILNKNGYSCALLTGSTPKS
                     QRDPLLANLANGKINVLIGTHAVFESPVIFQNLGLVIIDEQHRFGVDQRLKLREKGKN
                     PPHTLSMTATPIPRTLALTAYGDLDITTIKEMPKGRPEIITKVLRPNQRELLYEALRL
                     ELKAGRQAYVIYPLVEESEKLDLQNAVDGAEHLANGPLAGFRLGLLHGRMKANDKATV
                     MEQFKKGIVQALVSTTVVEVGVDVPNATVLAIENAERFGLSQLHQLRGRIGRGTELSH
                     CFLITSTNAGPSYERLCAMEETRDGFKLAELDLEIRGPGEFLGTKQSGELQFRYASLV
                     RDKDTLVEARQAAFSLLALDPELRLPENNGLRNYMLRAGHLSQSRYDTA"
     gene            3004..3894
                     /locus_tag="PLH57_02975"
     CDS             3004..3894
                     /locus_tag="PLH57_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRK01602.1"
                     /translation="MTRNGFIKSFDGTRIYFEVQGSGPPLLFCYGLVCSKLHWSYQMD
                     YFKQRYTVIWADYRGHHLSEMPKKTEALTVENMGRDLVCLLDELGIKKTAVLGHSMGV
                     NVVLELFRTAPDRIASIVLANGSARSPLESLFRTNIMQTIVPKIYALYKRAPKLLNFI
                     WSLQGKTRWTSAVVGMLGFNPHLARKEDIETYVRMVTEMDMIVTLQLLSDYERYDATA
                     WLDRINVPTLIIAGENDIIIPREAQEVLHQLIPESRYFLVRNGSHCPQMDIPDLINLT
                     IDRFFGEVYPSPTSITSSTQ"
     gene            complement(3855..>4785)
                     /locus_tag="PLH57_02980"
     CDS             complement(3855..>4785)
                     /locus_tag="PLH57_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRK01603.1"
                     /translation="VASLECLENIPCRACADACPENAISILDLADQPTLLEDKCTGCG
                     ICVAVCPAGAAAMIRENTADQKVRLFLPDHTKDIWKPQTAVQLLNRRGDSLTLARVVA
                     SQAYVNAPHRVLEIEVSNVHLWEGRAFRPMQNQHEGDLSAALADPQSLKRSWVTLNGS
                     KRLCPTGVPLTVALWQLGQKRFEDAFFCEDGSCRMCRVRVDGVDRLACQMIVAEGQTI
                     EFNSQYSGESWVCPCSRVKAEELDLNKSEGLPQKLTAEITGFSLGTCKGKWCLNECSQ
                     FGEDQKPRPAFQGFESSPWRDYWVDDVIEVGEG"
BASE COUNT         1333 a         1191 c         1078 g         1183 t
ORIGIN      
        1 ttttaatcgt tctcctgtac cgaacgacgc agaagttttg ttaaaaacac tattggcaat
       61 taaatacgat ttggatgcat ccatcatcaa tcaacatata gcgtcggcgc taccaagcac
      121 aggcgggcgt ttggactccg caattcaagt cgtagccatg gccctagcaa atcaacagca
      181 gaaatccgcc ccgttggacc ggcacgaact gcgggaactg ccaccggctt cggttcagtc
      241 aacgtcagca ttcgatttaa ttttgctcaa gccgatttac caaggctttg agggtgaaaa
      301 ctaataacag gtaccgtttt tgtttttttg tgataggaaa tattcgatat gagtgaaaac
      361 gattcggtaa aaccagtttt gtcctcggca gtacccatga ccatcgaagg caagcgaatg
      421 ctagaagatg agctcaaacg cttgcgttcc gtagagcgcc cgtcggtcat taaggccatc
      481 gaggaagcac gcgcgctggg tgatctttcg gaaaacgccg aatactcggc agccaaagaa
      541 cgtcagtcct tcattgaagg tcgcattcag gagcttaatg ccaagctcgc ttcagctcag
      601 gtaatagacc ccacaaccat ttccactgac aaagtggttt tcggtgcgac cgtggtactt
      661 tctgatcaag attctggcaa agaagtgacc tataaaatcg taggtacaga cgaatcagac
      721 atcaaacaaa acaaactttc agtgagtgcg ccgctcgcgc gagcacttat tggaaaaaaa
      781 gttggggacg aagtcacagt caatgctcca aaaggatcgc ttctctacga cgtcgtcgaa
      841 atccggtacg agtaaatcgg ccgcggccgg tctccacctg cgatctcccg tttctgcgct
      901 taaaggcgtt ggtcctaaga tcgcccattc actagaagct aagggcattc gcactatcga
      961 agacctcgtg ctttatcttc cacgagatta ccaagatcgc tcacaactaa aaccaatttc
     1021 tcaactcatt gccggagaac gggcacttgt tcgcggcact attcgaaaac atcgattcgt
     1081 ttatcttcga ggaaattccc gaggctttac ccagcccttg ctggaagctc aacttgatga
     1141 tggcagcgga cgcgtgatca tgctcaaatg gtttcgatat aatcggaaat tttttgaaac
     1201 caaatttgcc gaacagcctt ctatcttggt ttacgggcaa attcgcagtt tcggccacaa
     1261 tttcgaaatc attcacccgg acatcgattg ggctggcgac gattctggtg ccaacgcgga
     1321 acgtattgtg ccggtttaca gcgaaatcga aggggtgcac caaaagctct taagacgaat
     1381 tattgcgtct gcagttgcaa taggagttcc aaaacttgag gaagaaatac ctcaatctat
     1441 tctaactacg caggacttgt taccacttaa agacgcagtt ttatttttac atcacccacc
     1501 taaaaatttc ccaaccgatc aattgaaaac ttttgcaagt ccagcccaac gcaggttggt
     1561 gttcgaagag ctttttaagt ttgagtggat tattgggcaa aagcgtatga atatccgcaa
     1621 agagccgact gtcggatttc ctaccaatcc tgcgctggga cttttgggag aaatggaacg
     1681 tcaaataccg tttcaattaa ccggcgacca gaaagttgcg atatctaaaa tttatgatga
     1741 tctcgctcaa aaaaaaccaa tgaatcgcct tttgcaaggc gacgtcggct gtggaaaaac
     1801 attggtggca cttgcctccg ctttgcctcc attggctgcc ggcgctcaaa ccgtaatcat
     1861 ggctccaact gaaattttag cggaacaaca tttcaaaaac gcactcaata ctgtgggtcc
     1921 gattttaaat aaaaatggtt acagttgcgc actgttaacg ggatccacac caaaatctca
     1981 gcgagatccg ctattggcga atctcgcaaa cggcaaaata aacgtgctaa tcggaacgca
     2041 tgcggttttc gaaagcccgg tcatattcca gaatctcgga ctcgtgataa tagacgaaca
     2101 acatcgtttc ggtgtcgacc agcgactaaa attgcgagaa aaggggaaaa atccacccca
     2161 tacgctttca atgacggcca ccccaattcc gcgaactttg gcgctgaccg catacggtga
     2221 tctcgacatc accacaatca aggaaatgcc aaagggcagg cccgaaataa ttaccaaggt
     2281 acttaggcca aatcaacgcg agcttttgta tgaggctctc agattggaac ttaaagctgg
     2341 tcgccaggca tatgtaattt atcctttggt agaagaatcc gagaagcttg atcttcaaaa
     2401 tgcagttgat ggcgcagaac atttggcaaa cggtcctctc gcaggttttc gcctcggact
     2461 cctccacggt cgaatgaaag caaatgacaa agcaacagta atggaacaat tcaaaaaagg
     2521 aatagtgcag gcactggttt ctactacagt cgtcgaagtc ggggtcgatg ttccaaacgc
     2581 caccgtactc gccatagaaa atgccgaacg atttggactt tcgcaacttc atcagcttcg
     2641 cgggcgcatc ggacgaggta ccgagttatc ccattgtttc ctaattacat cgaccaatgc
     2701 aggtccgtct tacgaacgac tgtgcgctat ggaagaaacg cgagatggat ttaaactagc
     2761 agaactggac ttggaaatac ggggtccggg agagttttta ggtaccaaac aaagcggcga
     2821 actacaattt cgctacgcca gccttgtgcg agacaaagat accctggttg aagcccgcca
     2881 agcagctttc tcacttctgg cgcttgaccc cgagcttcgc ctaccggaaa ataacggact
     2941 gaggaactac atgctgcgag ccgggcatct tagtcaaagt cgctacgaca cggcataaag
     3001 aaaatgacac gaaacggatt cattaaatct ttcgacggca ctcgcatata cttcgaagta
     3061 caaggtagcg ggcccccgct gctattttgc tatggtcttg tttgctccaa actgcactgg
     3121 agctaccaaa tggactactt taagcaacgc tataccgtca tttgggctga ttaccgaggt
     3181 catcatctct ctgaaatgcc aaaaaaaact gaagcactaa ccgtagaaaa tatgggtcgc
     3241 gatctagtct gtttacttga tgaacttgga ataaaaaaaa ccgcggtatt gggccactcg
     3301 atgggtgtaa acgtagtgct tgaattgttt cgcacagcgc cagatcgcat tgcatcgatc
     3361 gtgctcgcca atggaagtgc ccgaagccca ctggagtcgc tctttcgcac caacatcatg
     3421 cagacgatag ttcctaaaat ttacgctcta tataaaagag ctccgaagct tttgaacttt
     3481 atttggtctc tgcaaggtaa gacgcgttgg acatcggccg tggtaggaat gttgggtttt
     3541 aacccgcatt tagcgcgcaa ggaagatatt gaaacctacg ttcgcatggt gaccgagatg
     3601 gacatgatcg tgacgctaca actgttgagt gactacgaac gttatgatgc tacagcttgg
     3661 ctcgatcgaa taaatgtccc tactttgata atcgctggtg agaatgacat tatcattccc
     3721 cgcgaagccc aagaggtact tcaccagctc atacctgaga gtcgctattt tctggttaga
     3781 aatggtagtc actgcccgca aatggacatt cccgacctaa taaaccttac tatcgatcgt
     3841 ttttttggag aggtttaccc ttcgcctact tcgatcacat catcgaccca atagtcgcgc
     3901 caaggagaac tttcaaaacc ttgaaatgcg ggccgaggtt tttgatcttc tccaaattga
     3961 gagcactcgt ttaggcacca tttaccttta caggtaccca atgaaaaacc agtaatctcg
     4021 gcagttagtt tttgtggaag tccctcactt ttgttcaagt ccagctcttc agcttttacg
     4081 cgactacacg gacaaaccca actttcgcca gaatattggg aattaaattc tatagtttgc
     4141 ccctctgcga cgatcatctg acaggccaat cgatcgacac catccactcg cacgcgacac
     4201 atacggcaag aaccatcttc gcagaaaaaa gcgtcttcaa atcttttttg gcctagctgc
     4261 cacaaagcga cagttagcgg caccccggta gggcacaatc gtttgctgcc atttaacgta
     4321 acccaggatc tttttagaga ttgcgggtcc gcgagtgccg cactcaaatc accttcgtgt
     4381 tggttctgca ttggacgaaa cgctcgcccc tcccaaagat gcacgttgga aacttcaatt
     4441 tccaatactc ggtgcggagc gttcacataa gcctgactag caaccacacg cgcgagagta
     4501 agagaatctc ctctacgatt taacagttga accgcggttt ggggtttcca tatatccttg
     4561 gtgtgatctg gcaaaaagag ccgaactttt tgatctgccg tgttttctcg aatcatcgca
     4621 gcagccccag ccgggcagac agctacgcat attccgcaac cagtgcattt gtcctctaac
     4681 aatgtgggtt gatcagccag gtcaagaata ctaatcgcat tttcgggaca tgcatccgcg
     4741 caagcgcgac atggaatatt ttccaagcac tccaaactcg ctact
//
LOCUS       DAOMXO010000091         4784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9509_length_4784_cov_4.741806, whole genome shotgun sequence.
ACCESSION   DAOMXO010000091 DAOMXO010000000
VERSION     DAOMXO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9509_length_4784_cov_4.741806"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLH57_02985"
     CDS             <1..631
                     /locus_tag="PLH57_02985"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRK01604.1"
                     /translation="VNNPAAWAHSQKMVMEAMDEAGLDYYEGLGEAAFYGPKIDIQFR
                     TVTGREETASTCQLDFAMPERLDLAYKGADNGLHRPYIIHRAPLGTHERFVAFLIEHY
                     GGAFPTWLSPVQVMIVPVSDVFTEYSQKLVDELRSDYVRVELDATNDTLNKRLRNAVT
                     RKIPNILVIGEREQANSTVTLRRYGIQEQKTMAAAEFRNWLAETIRNRS"
     gene            644..1288
                     /locus_tag="PLH57_02990"
     CDS             644..1288
                     /locus_tag="PLH57_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK01605.1"
                     /translation="MPIYTFRRKLRELTQTFNVYHYQQYMSLIVAPTRKRAFDELKLT
                     NETKLLEVGVGNGSSLEFFPAGIQITGIDFSATLIEQARIRAKELQMSRARFYVMNAQ
                     NLRPLESNSFDAVVSLSLLSVVPEPEKAFSEMVRVCRPGGKIAVTSHFLHKSGWRKSV
                     DQILSPAVESILGYHVTLPEELIVARKDLRVLVREDRRIGPYVMNSVFVVEKLN"
     gene            complement(1280..1747)
                     /locus_tag="PLH57_02995"
     CDS             complement(1280..1747)
                     /locus_tag="PLH57_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01606.1"
                     /translation="MSSATKSSKTARSSKFAQTWLLEPIECDPTFETRRMFGGLAVYL
                     GGRMVLVLMESDPSDLEYRGKKFDFPIWSGLLIPTDRTHHDSLLRDFSDLRVHPVLGK
                     WLYLVMQDDYEKTAQEIVDAIGKGDSRFGIEPGTRARKRKKVIRVKSVRKRLV"
     gene            complement(1807..2166)
                     /locus_tag="PLH57_03000"
     CDS             complement(1807..2166)
                     /locus_tag="PLH57_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK01607.1"
                     /translation="MQLKIYYLDDESDLCEIFKDNFESDSVVIQTFTDPELAFLATKN
                     SPPHLIFLDFRLPGTTGDQIASRIGPSVPKVLVTGELDIKPESSFVKIFSKPFEFSEM
                     AEFLKDFAAQANAREKK"
     gene            complement(2156..3964)
                     /locus_tag="PLH57_03005"
     CDS             complement(2156..3964)
                     /locus_tag="PLH57_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK01608.1"
                     /translation="MFQNRAHLKFVAPKYPLVPTLVIRAVLLGALIGTVILITMNNRY
                     FQVGMNEVDQLADIHLNIVKNWDTIASAAKLGSTIGHVAFENRYIEAEAQIHENLKTL
                     GSLSKEAHTPLKAELATVTKIVADLQNQISKLDYQAFDLTRTNGGEAARKILFGVNYE
                     KLQNEMLTAQNRLEKVVNSYQRHTMKDIATRYNRQMVLLVLALFAEVVSTVIILLLNK
                     KWGLKVQQINKSLEDVVQQRTKDLVDAQAIAKIGSWSLEPDTRGLSWSSELYSIFEIA
                     NPQPKDSLFQQYRSRIHPEDVLLLEQLLENAAKNSEDFTFEHRICFGDDQIKYVRTIG
                     KVVKSVNGRPSLINGTCQDITEEVKNKNALDLEKNKSLRSSKLASLGEMSAGIAHEIN
                     NPLAIIAGASNALPKFLNDPEKIKEKLVTIDRAVRRIAKIVKSLQKFARSSERQSYKQ
                     YSLNNIIKEALILTESKSKRHSTPVTVDCRTEGQILCDEIEIEQILINLINNGIDAVG
                     ALTEKWVRITLFEQNNYVILQVRDSGSGLPREVKKNLFQPFFTTKQVGKGTGLGLSIV
                     KGILDEHQASIDILENDANTCFEIRFNKAEVVQNAA"
     gene            complement(3964..4497)
                     /locus_tag="PLH57_03010"
     CDS             complement(3964..4497)
                     /locus_tag="PLH57_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01609.1"
                     /translation="MSNLFLLLAFIGSAAWAQEVPKARIDEAMKELVQMASDPRLVEL
                     VVSYNTNPPSEYVSMTNEAWSKLSSRDPLVKKLTQNEAAKLIRSQIKSYVSEAFLSDR
                     KGGKVALLSKTSRWSHSGSPKHETPMSGKNWYGKPELDKSSGVNQVQISAPVLYRGKP
                     VGSLVLGLSIVKLLGNE"
BASE COUNT         1255 a         1159 c          996 g         1374 t
ORIGIN      
        1 tgttaataat ccggcggcct gggcccactc ccaaaaaatg gtgatggaag ccatggacga
       61 agcgggcctc gattactacg agggtctggg tgaagctgcg ttttacggac cgaaaatcga
      121 tattcagttt cgcaccgtta cggggcgcga ggagacagcc agtacctgtc agctcgactt
      181 tgcaatgccg gaaagactcg atctcgctta caaaggcgcc gataatggat tgcatcgccc
      241 ttacattatc caccgcgctc ccctcggcac tcacgagcgg tttgttgcat ttttaatcga
      301 acactacggc ggagcttttc ctacttggct ttcgccggtt caggtaatga ttgttccggt
      361 gtcagatgtg tttaccgagt acagccaaaa actggttgat gaactacgca gtgactacgt
      421 tcgggttgag ttggatgcta ctaacgacac cctcaataag cggctccgca acgcagttac
      481 tcgaaaaatt ccaaacatcc tagtaatcgg tgaacgagaa caagccaatt cgactgtgac
      541 tcttcgtcgg tacggcattc aggaacaaaa aactatggct gccgcagagt tccgaaactg
      601 gttggccgaa actattcgga accgcagcta gaaggtagca aaaatgccaa tttatacatt
      661 tcgccgaaaa ctccgcgaac taactcaaac tttcaacgtg tatcactacc aacagtacat
      721 gagtttgatt gttgctccaa cgcgcaagcg cgccttcgat gaacttaagt taaccaatga
      781 aaccaaactt ctcgaagtcg gagtgggcaa tggcagctca cttgaattct tcccggctgg
      841 cattcaaatt acgggtatcg acttctcggc aacacttatt gaacaggctc gcattcgggc
      901 taaggaactg caaatgtccc gagcgcgttt ctatgtaatg aatgcacaga atctacgccc
      961 gctcgaatca aactcctttg atgcggtcgt atctctttca ctactatcgg tagttcctga
     1021 accagaaaaa gcattctctg aaatggtccg cgtctgtcga cctggcggca aaatcgctgt
     1081 aacgagtcac tttcttcaca aaagcggctg gcgcaaatca gtggatcaaa tcttgtcccc
     1141 agcggtcgaa tcaattctcg gataccacgt aactctgccc gaagaactaa tagtagcccg
     1201 aaaagaccta cgggtgctcg tgcgtgaaga tcggcgcatt ggaccctatg tcatgaactc
     1261 ggtatttgtt gtagaaaagc taaactagcc gttttcgtac ggactttacg cgaataactt
     1321 tttttctttt gcgagcacgc gtcccgggtt cgatcccaaa gcgcgagtca ccttttccga
     1381 ttgcgtctac aatttcttgc gcagtttttt cgtaatcgtc ttgcatcacc aaatacagcc
     1441 acttgcctaa tactggatgc actcgcaggt cgctaaaatc tctaagaagc gaatcgtgat
     1501 gtgttcggtc ggtaggaatg agtagtcccg accaaatcgg aaaatcaaat ttcttgccgc
     1561 gatactccaa atctgacggg tcgctttcca tcagcacaag caccattcgg ccacccaaat
     1621 acacggcaag cccaccgaac attcgtcgag tctcgaaagt cgggtcgcat tcaatcggtt
     1681 caagtaacca ggtctgagca aattttgaac tgcgtgcagt cttactagac ttcgttgccg
     1741 acgacataga ttcatctata ccacatggcg atcaacgatt cagcgttggc gtacactttt
     1801 ctagtactat tttttttcgc gcgcgttagc ttgcgcagca aaatctttta gaaactctgc
     1861 catttctgaa aattcaaatg gcttactaaa aattttcaca aacgaggact cgggttttat
     1921 atctaattcc ccagtgacta acaccttcgg tacagatgga ccgatgcgcg atgcaatctg
     1981 atcgcctgtg gtaccaggaa gtcgaaagtc caaaaatatc aaatgcggcg gactattctt
     2041 tgtcgccaag aatgccaatt ctgggtcggt aaaggtctgg atcaccactg aatctgattc
     2101 aaagttatct ttaaatattt cacaaagatc tgattcgtcg tctaaataat atatcttaag
     2161 ctgcattttg aactacctca gctttgttaa agcgaatttc gaaacaagta ttggcatcat
     2221 tttctagaat atcgatcgaa gcctggtgct cgtcaaggat cccctttacg atagaaagtc
     2281 caagtcctgt ccctttgccg acttgtttgg tggtaaaaaa tggttggaac aaattctttt
     2341 ttacttcgcg tggcaaacct gaaccggaat ctctgacttg aagaatgacg taattattct
     2401 gttcaaacaa ggtgattcgc acccattttt cagtcagtgc acctactgcg tcaatgccgt
     2461 tattaattaa attaattagt atttgttcaa tttcgatttc atcacaaaga atctgaccct
     2521 cagtccgaca atctacggta acgggagtag aatgtcgttt tgatttggac tccgtgagta
     2581 tcaacgcctc ttttattatg ttattaagag agtactgttt ataactttgt ctctcggaac
     2641 tacgcgcaaa cttttgcaag ctctttacga tttttgcaat tcggcgcact gctctatcga
     2701 tcgtgacgag tttttcttta attttttcgg ggtcgtttaa aaactttggt agcgcgttcg
     2761 atgcacctgc aataattgct aacgggttat taatttcgtg agcaatgcca gcggacattt
     2821 caccaagcga tgccaacttc gatgaccgca ggcttttatt tttttcgaga tcaagtgcgt
     2881 ttttattttt tacctcttcg gtaatgtcct gacaggttcc attgatcagc gacggccgac
     2941 cattaacact cttaactacc tttcctatgg ttctaacgta ttttatttgg tcgtcgccaa
     3001 aacaaatacg gtgctcgaag gtaaaatctt cgctattttt ggccgcgttt tctaaaagtt
     3061 gttcgagcaa tagtacatct tctggatgta ttcgagaacg gtattgttga aacaaactgt
     3121 ctttgggctg gggatttgca atctcaaata tagaataaag ctcactcgac caactaagac
     3181 cgcgtgtatc tggctccaaa gaccagctcc caatttttgc gatcgcttgt gcgtcaacta
     3241 aatcttttgt tcgctgctga acgacatctt ccaaactctt attgatttgc tgtactttta
     3301 atccccactt tttattcaac agtaaaataa tcacggtcga cactacttcc gcgaataacg
     3361 ctaaaactag caagaccatt tgtcgattgt aacgggtcgc tatgtccttc atcgtatgac
     3421 gttggtagga attcactact ttctctaagc gattttgagc agttaacatt tcattttgca
     3481 gcttttcata atttacgccg aaaagaattt ttctagcagc ctccccaccg ttggtacgag
     3541 taagatcgaa ggcttggtaa tcaagtttcg aaatttgatt ttgcagatcc gcgactattt
     3601 tcgtaaccgt ggccaattcg gctttaagag gcgtgtgtgc ctctttgcta agactcccta
     3661 gggtttttaa attttcatgg atttgagctt ctgcctcgat gtaacgattt tcgaatgcca
     3721 cgtggccaat agtacttccg agctttgcag cgctcgcgat ggtatcccag tttttaacga
     3781 tattcaggtg aatatcagct aattgatcca cctcgttcat gccaacctgg aagtatctat
     3841 tgttcatggt aataagaatc accgtaccaa taagagcccc aagcagcacc gcgcgaataa
     3901 cgagagtcgg caccagcgga tatttcggcg ctacgaattt cagatgggcg cggttttgaa
     3961 acactattca tttcctaaaa gtttaacaat tgataggccg agcaccagcg atccgacggg
     4021 tttgcctcga tacagaacag gggcagaaat ctgaacctga ttaacccccg aagacttatc
     4081 tagttccggt ttgccatacc aatttttgcc cgacatgggc gtttcatgct tgggactacc
     4141 actgtggctc catctcgacg ttttacttag caacgccact tttccgccct ttcgatctga
     4201 gagaaaagcc tcggaaacat aagatttaat ctgactgcga atcagtttcg ctgcttcgtt
     4261 ctgggtaagc ttttttacca atggatcgcg actcgacaac ttcgaccacg cttcattggt
     4321 catagatacg tattccgaag gcggattggt attgtacgaa accaccaatt caaccaacct
     4381 gggatcggac gccatctgca caagttcttt cattgcttca tctattcggg cttttggaac
     4441 ttcttgggcc cacgccgccg acccgatgaa cgcaagtaac aaaaacaagt tcgacatcac
     4501 actcacccct cccctccttg attattcggt attttcgcaa ttctctttaa aatttgagct
     4561 catcggccca aaaagaaaaa cgaccgatgc cccacacaac tgtcaaaaga ttagacagtt
     4621 tttgtgagta aaatccaata tttccaacgc ctccccccga attctcctgg cttgtgattt
     4681 gcaggaacat aggtctcgag gagaagagta tgacaacgtt ttttcgcatc gccactcaat
     4741 ggcgcgtaat tcaggtaacc ctttttctat tcaccctttt atta
//
LOCUS       DAOMXO010000092         4784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9510_length_4784_cov_4.088179, whole genome shotgun sequence.
ACCESSION   DAOMXO010000092 DAOMXO010000000
VERSION     DAOMXO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9510_length_4784_cov_4.088179"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PLH57_03015"
     CDS             <1..792
                     /locus_tag="PLH57_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01610.1"
                     /translation="EVRNKKTLVLLFLSLTVAAISWGCGGGGDPAGFDPATNCSPDSP
                     TFDPISCNGGGTGLPEGAGNFNGGRDGLAAIRNEYLGSEYNLKEQDKKVPKLAATAQG
                     VDGGFGAGESGGNSNNNGAGGAMSLPKVSFAKSGGGSMNGGTGSDDRLTANENKRSVA
                     SDEEESKTLSKSDGFGDAAKGGGGSASGEGSIFGSLFGSKGLGGGAGAGAGESEGAGD
                     LDAYLKLVGNKSLFEIVNRRYDRWSTELEISRNPAQYESGRGAAF"
     gene            991..1914
                     /locus_tag="PLH57_03020"
     CDS             991..1914
                     /locus_tag="PLH57_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRK01611.1"
                     /translation="MTWNVQFLAGKGYVFFFDVLDGSGPDTRPDRSAVSATLAEVIRV
                     VREESPDFVLLQEVDEGAKRTDYQDQIALIYQGLREVYPCYATTYYWRSKFVPHPKIW
                     GAVGMKLATLSRYHIKSARRVQLPVMPGLWWKQQFELKRAVLDVEIASESKFPLHVLN
                     THLDAFAQGTDTMRQQVERIAGMLATYDYEGIPWILGGDFNLLPNREAFDELPEFRKS
                     YYNSETELNTLIQKYQSVPDTRSASNRRLEWRTHFPNDPRVKAPDSIIDYLFVSPKVE
                     ILRRRVRQDDTFKISDHFPVIGELLIKEKQK"
     gene            complement(1877..3589)
                     /locus_tag="PLH57_03025"
     CDS             complement(1877..3589)
                     /locus_tag="PLH57_03025"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HRK01612.1"
                     /translation="MPYGGEQLLREFGEMAYAVKRLHWIFLVVAGAVAIHGLVPALVE
                     HKLVAGHDTAAYAIYATEFHENIRSGNWFPIWAADLRFGHGEPHLAFRPPLQLFLTEF
                     IFTVLRDPITAVNWTMVVILLGALAAMYAFLRDHTTRTVAALGASVYGLSPYLLRDVY
                     IRGALSEATAFVVLPLLALTSLRLIRRPSRTNFIFYALSWALLASCHAAILLIATPFF
                     VAGILIYYKSFGLRKHSAWQKLLAGFLFGLGSSSFYWLSALLEQKHVASPLIGVDFEA
                     YIWHFKQLTQILFSSFTSIKNKTLAYNVGEPILFALLLFPFVAWRNHVSRLLALGMVI
                     VFYFMHQTSAIWYELLPWLQSMTYPWRFLTMATFLGALLFSCLINLQAKWIEKKVAIT
                     FLIFLSLTAFGFIHAKAPALYTFTGHTFSPSSLASTGTGWAKPYTVPASTGFMPTSKP
                     ESWLDFVSGRGEAQCKRPSPTRIFCEVMAIQPAIIRFNVLDYPGWTVVRENTQIEKIT
                     EPGFGRILVELPHGKQTLELILKNTGARWFTKTLSILSFLGIFVLVFFRKATFASLLS
                     KARR"
     gene            complement(3549..>4784)
                     /locus_tag="PLH57_03030"
     CDS             complement(3549..>4784)
                     /locus_tag="PLH57_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01613.1"
                     /translation="WFLISFGPATWGTFLTSLPFATNMHWHRAFLMFQITLAACTGIA
                     FVSFLSALNKVFTEKINPPQPQYWTAGIAATLVSLVFAGPLVERHRYFYKTNAHWLSE
                     SKKSWTDNLASQDLLRFAQSKTGTRFYAGTHPNVLPGSWGNDLRVTTYIPVYGLFAQH
                     NVESVGPIYHHQNHTEDASFRYLWKSKAHNELFNIGYLVANDKQTMPPEFKNTNKFGS
                     ASVYTTGIQENYFAFAKIFPENAFLPDAALPCADNAGLVRATLEFLFSRLPETGQFPA
                     FQLQRRCRTSNELDQFLKDLKKSSDVPPKGQFTAAGRATGKSSYNYWAEGQNETATAL
                     VFKMNFHPLWSLKINGVEVEKRLVSPALIAVPIPAGHFRIEAEYQPGRLRLIFATLTL
                     AFGGVLIALRRRTALAGIW"
BASE COUNT         1336 a         1024 c         1233 g         1191 t
ORIGIN      
        1 gaagttcgga ataaaaagac gttggtgctt ttatttcttt cgcttacggt cgctgcgatc
       61 tcttggggtt gtggtggtgg tggggacccg gctggctttg atccggctac aaactgttca
      121 cctgattcgc cgacctttga tccgattagc tgtaacgggg gaggaaccgg actaccggag
      181 ggggctggaa actttaacgg cggccgcgac ggtttagctg cgattcgaaa cgaataccta
      241 ggaagtgagt acaatctaaa ggagcaagat aaaaaagtac ccaagttagc tgcgactgcc
      301 caaggcgtcg atggtggttt tggtgccggg gaaagtggcg gcaattcgaa caacaatgga
      361 gccggcgggg ccatgtcgtt accgaaggta agttttgcca agtccggtgg cggttcgatg
      421 aacggtggta ctggtagcga tgaccgcctg actgctaacg aaaacaaaag aagcgtagct
      481 tccgacgaag aagaatcaaa aacgcttagc aaatcagatg ggtttggcga cgccgcaaaa
      541 ggtggcggag ggagcgcttc tggcgagggc tctatatttg gaagcctctt tgggtctaaa
      601 ggacttgggg gcggcgccgg agccggtgct ggtgagtccg aaggagcggg agacttagac
      661 gcctatctca aattggtcgg gaacaagtcg ctttttgaaa tcgtaaaccg gcgctacgat
      721 cgttggagca cggagcttga aattagtcgc aatccagctc agtatgaatc tggtcggggt
      781 gcagcttttt aaactgatac ggccctctct cttggtgacg tgcgtataaa aaaaacactt
      841 agaattgtag cggggctgtt tcttctactt acagttcttt tcactgtatt tgcatttagg
      901 gttaatgctc gattcggagc tgtcgaaaca gaaccggtaa attgctctac gaaagcccca
      961 ctctttaagt ttccccaaga aatcaaagtg atgacatgga acgtacagtt cctcgcgggc
     1021 aagggttacg tctttttttt tgatgttcta gacggctcgg ggccagacac ccgaccagat
     1081 cgatccgccg tctctgcaac cctggccgag gtgattcgcg ttgttcgcga agaaagtccc
     1141 gactttgttc tgttgcaaga agtagatgag ggcgcaaaaa gaacggatta ccaagatcag
     1201 atcgcactta tctaccaggg gttgagggaa gtgtacccct gttacgcgac cacctattat
     1261 tggagatcaa agtttgttcc acatccaaaa atttggggtg cggtaggtat gaaattagcg
     1321 acgttgtcga ggtaccacat taaaagcgcg cgacgtgttc aacttcctgt catgccaggg
     1381 ttgtggtgga aacagcagtt cgaactaaaa cgcgcggtgc tcgacgtgga aatcgcaagc
     1441 gaatctaagt ttccacttca tgttttaaac actcatctag acgcctttgc tcagggcacc
     1501 gatacaatga gacagcaagt tgaacgaatt gctggaatgc tagcaaccta cgactacgaa
     1561 ggaattcctt ggatattagg cggtgatttt aatctactac caaatcgtga agccttcgat
     1621 gagttgccag agtttcgaaa atcctattac aattctgaaa ccgagttaaa tacgttaata
     1681 caaaaatacc agtcggtacc cgatactaga tcggcatcaa atcgacgact agaatggcga
     1741 actcattttc caaatgatcc acgagtgaag gctccagatt cgataatcga ctatctattt
     1801 gtttctccaa aagtcgaaat actccggcgg cgtgttcgtc aggatgatac gtttaaaatt
     1861 tctgatcact ttccggttat cggcgagctt ttgataaaag agaagcaaaa gtagctttcc
     1921 ggaagaaaac caaaacaaat attcctaaga aagacaaaat agaaagcgtc ttggtaaacc
     1981 aacgagcacc ggtgttcttg agaataagct ctagcgtttg tttaccgtgg gggagttcga
     2041 ccaggatgcg accgaacccc ggttcagtaa ttttttcgat ttgtgtattt tcgcgcacta
     2101 cagtccaccc cggatagtct aaaacattaa agcgaattat cgccggttga atggccataa
     2161 cttcgcaaaa aatccgtgtg ggcgatggtc gtttacattg agcctctccc cgaccgctaa
     2221 caaagtctaa ccaagattcc ggtttggagg ttggcataaa accggtacta gctggaacag
     2281 tgtagggctt agcccatccg gttcccgtgc tagccaaaga actcggcgaa aacgtgtgac
     2341 cggtaaaagt atataacgcg ggtgctttgg cgtgaataaa accaaatgcc gtcaggctca
     2401 aaaaaattaa aaatgttatc gcgacctttt tttcaatcca tttggcttgc aaatttatta
     2461 ggcaggaaaa caaaagggca cctaaaaagg tggccatggt taagaatctc caagggtagg
     2521 tcatgctttg aagccagggg agtagttcat accaaattgc agaagtttga tgcatgaaat
     2581 aaaaaacaat caccattccg agtgccagaa gacgagaaac gtgatttcgc catgccacga
     2641 atggaaataa taatagcgca aaaagtattg gttctccgac attgtaagcc agggttttgt
     2701 tttttatgga agtaaacgaa gagaataaaa tttgagtgag ttgcttaaag tgccaaatat
     2761 aggcttcgaa atcgacgcca attagcggtg aggcaacatg tttttgctct aatagagcgc
     2821 ttaaccagta gaatgaagaa cttccgagac cgaaaagaaa accggcaagc aacttttgcc
     2881 acgctgaatg ttttcgtaat ccaaaacttt tgtagtaaat gagaattcca gccacaaaaa
     2941 acggagttgc gattaatagg atcgcggcat ggcaactagc gagcagggcc cagctaagag
     3001 cataaaaaat aaaattagtt cgcgaaggtc tgcgaatcaa tcgaaggctt gtaagggcta
     3061 atagcggcaa aacaacgaaa gccgtagcct cgctcagcgc gccgcgaatg tatacatcac
     3121 gaagtaaata cgggcttagc ccataaacgc tggcaccgag agccgctacg gttcgagtgg
     3181 tgtggtcgcg caagaatgca tacatggctg ctagtgcacc caaaagaatt acgaccatag
     3241 tccaattaac cgctgtgatt ggatctctta aaactgtaaa aataaactcg gttaggaaga
     3301 gctgcaaggg tgggcgaaac gcaaggtgtg gttctccgtg gccgaacctt agatcggctg
     3361 cccaaatcgg aaaccagttt ccgctgcgaa tattttcgtg aaactctgtc gcgtaaattg
     3421 cgtaggcagc ggtatcgtgg ccggccacga gtttgtgttc cacgagtgct ggaactaagc
     3481 cgtgaatagc cacagccccg gcaactacca gaaaaatcca atgcagacgc ttcaccgcgt
     3541 acgccatttc accaaattcc cgcaagagct gttcgccgcc gtaaggcaat gagcactccg
     3601 ccgaaggcta acgtgagtgt ggcaaaaatt agccgtagac gcccgggttg gtattcggct
     3661 tctattcgaa agtgtccggc gggaatggga acggcaatca acgctggtga aacgagtctt
     3721 ttttcgacct ctacgccgtt tattttcagc gaccatagtg gatgaaagtt cattttgaac
     3781 actaatgcag tggcagtttc gttttgacct tccgcccaat agttgtagga gcttttgccg
     3841 gtagcccgac cagcagcagt aaattgcccc ttcggaggta cgtcagaact cttctttaga
     3901 tctttgagaa attgatcgag ttcgtttgaa gtgcggcacc gtctttgaag ttggaaggcc
     3961 gggaattggc cggtttccgg cagtcttgag aataaaaatt cgagagtggc ccgaaccagg
     4021 ccggcattgt cggcgcaagg aagagctgca tcgggtagaa atgcgttttc cggaaatatt
     4081 tttgcaaacg cgaaatagtt ttcttggatt ccagttgtat aaaccgacgc actgccgaat
     4141 ttgttcgtgt ttttgaattc cggcggcata gtttgtttgt catttgctac gaggtagcca
     4201 atgttgaaga gttcgttgtg cgccttactt ttccaaagat aacgaaatga cgcatcttct
     4261 gtatggtttt gatggtgata aattgggccg acagattcaa cgttgtgttg tgcgaaaagt
     4321 ccgtaaactg gaatataagt tgtcacacgt aaatcgttac cccaagagcc aggtaaaacg
     4381 ttgggatgag ttcccgcgta gaaacgggtt cccgttttgc tttgagcgaa acgtaataaa
     4441 tcctgacttg ctaaattgtc tgtccaagat tttttacttt ctgaaagcca gtgagcgttg
     4501 gttttataaa aatacctgtg gcgttcaacc agcggccctg caaaaacaag gctcacgaga
     4561 gtagccgcga ttccggcggt ccagtattgt ggttgaggcg gattaatttt ttctgtgaaa
     4621 actttgttta atgcggaaag aaaagaaaca aacgctattc ccgtgcatgc agcgagtgta
     4681 atttgaaaca ttaaaaatgc tcggtgccaa tgcatgttgg ttgcgaacgg taaacttgta
     4741 aggaaggtac cccaggtcgc gggtccgaaa ctgataagaa acca
//
LOCUS       DAOMXO010000093         4710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9728_length_4710_cov_5.664876, whole genome shotgun sequence.
ACCESSION   DAOMXO010000093 DAOMXO010000000
VERSION     DAOMXO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9728_length_4710_cov_5.664876"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="PLH57_03035"
     CDS             complement(<1..1138)
                     /locus_tag="PLH57_03035"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRK01614.1"
                     /translation="MATESKAILIIEDEASMVEMLEMALKKWGYRVRVARNLHDGLAA
                     VERGRVDLVLSDIKLPDGSGIDILGRVNTLPERPPVIFMTAFGSAESAVQAMKLGATN
                     YISKPFKLEALRELLQESLKKHHEVGDTGATGSVVNKIEEPLTATSGAKRGEFSRSNI
                     IGQSKALRELFQLVDRVAKTKTNILVTGESGTGKELVARAIHAGGPLKNTPFVAVNCG
                     AIPETLIESELFGHKRGSFTGAVADKEGLFQVANGGTIFLDEVGELPMSMQVKLLRVL
                     QDRTLRPVGGTEDIKVDVRVISATNRDLEQEIARGKFREDLYYRLNVIHIKSPPLRSR
                     KEDIPLLVDFFIKKFGLSIGKTIKTISAAAMNALMHYDFPGNVRE"
     gene            complement(1154..2731)
                     /locus_tag="PLH57_03040"
     CDS             complement(1154..2731)
                     /locus_tag="PLH57_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK01615.1"
                     /translation="MRAYRVYAEYLQHGVPNLPQPQLTADKDIRLYMFLRLLMLFSVL
                     TTLIWLQIIGVIERNVLNRSYILIGATFALTSALILFYEKAAKFRYFLTSQILYDVFF
                     TTALIFYSNPFESIYTVFYMFNVIFAAILFRGRGAIVTAILCSALYALLNSVAPQVNS
                     SEKVYSVLTVVTALLAVSMLSSRLFDELKMSQQKIGRLEALNDEIIDSLDSGLIAVDR
                     HEKIERINRTAAQLFEMQSVDSAYGKHLTSIFPILSTNDPNHITEFQINERQRRVVIS
                     KVELPESHKMYLIRDLTDVLELEEKLRRQERLAAVGQLAAGIAHEVRNPVASISGAAQ
                     LLSNTKDSEISSEERVRLIELIIREGDRIDRLIVQLLRFAKPSEHPRERFRLDEIIES
                     AREAILARTDLVQDSKITVSGETKIEIIGYRDQWYEVITNLLTNALQAGAKKIEIIAK
                     SQHKSAEVVISDDGKGIDSKVRSRIFDPFFTTKAQGTGLGLAHVHRVVREHDGQIDVE
                     SQLGRGTRMKIRVPLAA"
     gene            complement(2731..3939)
                     /locus_tag="PLH57_03045"
     CDS             complement(2731..3939)
                     /locus_tag="PLH57_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRK01616.1"
                     /translation="MPQFTYVAVNKEGQKIKGTVDSPNEGEVRIMLRSQQLRPIRISK
                     ASALEFDLAKIGGFGGKVKRADVLLFTRQLSVLLSAGIPLVQGLDIISGQASNNELKQ
                     ILGALREKISGGSFLWQAMGAYKDTFPDIFINMVKAGESSGSLDTILKRLIKYLDDAE
                     KLKKLVKGAMVYPIVVTAVGFIVLIVMMIFVIPKFEELLKSQNQELPGVTKFVIDASH
                     FFQKNLLFIVGGGVAVFYFFRSYLKTEEGKKFFDLIVLKAPLFGELVLKVSVARFART
                     MQTLLASGINLLDAIDICRTAVGNSTVEENLVKVRAEVENGKTLSSVMGRLPIFPSMV
                     VQMVTVGEGTGNMDKMLERVADYYEEDVENLVSGMTKMIEPLILVFLGGMVGGMLIAM
                     YLPIFTMAGN"
     gene            complement(3940..>4710)
                     /locus_tag="PLH57_03050"
     CDS             complement(3940..>4710)
                     /locus_tag="PLH57_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRK01617.1"
                     /translation="VMPRIALIATDPDCQRDTILKRPNGLVLVTGPTGSGKSTTLAAM
                     LDLLNQTESGHLITIEDPIEFIHPHKSCIVNQREVGTDTKTFKDALKRVLRQDPDFVL
                     VGELRDIETIEMALTAAETGHLVFGTLHTNNAVQTIYRIVNVFTPHQQPQVRQLLAFV
                     LQGIVSQQLIPKSNGTGRVLSMETMFPNSAIRNLIREDKLHQIYSTMQAGQNESGMMT
                     MNQSLVRLVQQGALTKEHAIELSQFPEELAKMLGMVRV"
BASE COUNT         1242 a         1131 c          946 g         1391 t
ORIGIN      
        1 attcacgcac attgccgggg aaatcgtaat gcattaacgc attcatagct gcagcagaaa
       61 ttgttttaat cgttttgcca atcgacagac cgaatttttt tatgaaaaaa tctactaaaa
      121 gcggaatatc ttcttttctc gaacgaagtg gcggcgattt aatatgaatc acattcaatc
      181 gataatatag atcttcccga aacttcccgc gggctatttc ttgttcgagg tctcggttcg
      241 ttgcgctaat gactctaacg tctactttaa tgtcttcggt gccgcccact gggcgcagcg
      301 tgcggtcctg caacactcgt aggagcttta cttgcataga cattggcaat tcaccaactt
      361 catctagaaa aatcgtgcca ccgttggcga cttgaaataa accttctttg tctgcgaccg
      421 ctccagtaaa cgaacccctt ttatgcccaa agagctcact ttctattaag gtttccggta
      481 tggcaccgca attgactgcc acaaatggag tgtttttcag cggcccaccc gcatgaatcg
      541 ctcgcgccac cagttctttg ccggtaccac tttcgccggt aactaatata tttgttttcg
      601 ttttagcgac tcggtctacg agttgaaaca actcccttag tgcttttgat tgcccgataa
      661 tattcgacct tgaaaactcc ccgcgtttcg caccagaggt tgcggtcagt ggttcttcaa
      721 tcttgttgac cacactaccc gtggctccag tatcgccaac ttcatggtgt ttttttagcg
      781 attcctgcaa caactcccta agagcctcta acttaaacgg cttcgagatg taattcgtgg
      841 cacccaactt cattgcctgc acggccgatt ccgcgcttcc gaaagcggtc ataaagatca
      901 ccggcggacg ctcgggcagg gtattcactc tgcctaatat atcgattccc gacccgtcag
      961 gaagctttat atcactcaac actaaatcca cgcgaccccg ttccacggcg gctaaaccat
     1021 cgtgaagatt acgcgcaaca cgcacgcggt agccccattt ttttagggcc atttcgagca
     1081 tttcaaccat actcgcttca tcttcgatga ttaaaatcgc cttgctttca gttgccatat
     1141 ttacaaactt tctttaggcc gctaacggca ctcttatttt cattcgggtt ccgcgaccta
     1201 attggctttc gacgtctatt tgaccatcgt gttcccgcac gactctatgt acgtgagcga
     1261 gccccagccc cgtgccctgt gcttttgttg taaaaaatgg gtcaaaaatt cgagatctta
     1321 ctttcgagtc gatgcccttg ccgtcgtcgc taatcaccac ttcagcactt ttgtgctgag
     1381 acttcgcaat aatctctatt ttttttgcac cggcctgcag tgcattagta agtaagtttg
     1441 taatgacttc ataccattga tcgcgatacc ctatgatttc aatttttgtc tcgcccgaca
     1501 cagtgatttt ggaatcttgc accaaatcgg ttcgcgctaa tatggcttcg cgtgcacttt
     1561 caataatttc atccagtcga aatcgttcgc gcggatgttc gcttggtttt gcgaaccgta
     1621 aaagttgaac aatcaatcga tcgattctgt cgccttcgcg aatgataagc tcgatcagcc
     1681 gcactcgctc ttcgcttgaa atctcggaat ctttggtatt tgaaagtaat tgcgctgctc
     1741 ctgaaattga agctaccggg ttacgtactt catgagcaat acccgctgcg agttgcccaa
     1801 ctgccgctaa acgctcttgt cgccgtaact tttcttcaag ttcgagaaca tcagtaagat
     1861 cacgaattag gtacatttta tgggactccg gaagctccac cttagatatc accacgcgtc
     1921 gctgccgttc attaatttga aattcggtaa tgtgatttgg atcgttcgta gaaagaattg
     1981 gaaatatcga agtgaggtgt ttgccatagg ccgaatccac cgattgcatt tcgaagagct
     2041 gtgcggcggt tcggttaatt ctttcaattt tttcgtgccg gtcaacagcg atcaatccgc
     2101 tatcgaggga atcgataatc tcgtcgttaa gagcctcaag ccgaccaatt ttttgctgag
     2161 acatttttaa ttcatcaaat aatctgctgg ataacattga gacggctaat aaagcagtta
     2221 ctacggtcaa taccgaatac actttttccg aactatttac ctggggggcc acgctattaa
     2281 gtagagcgta aagcgcgctg cataagatcg cagttacaat cgcgcctcga ccacgaaata
     2341 agatcgcagc aaatatcaca ttaaacatat aaaaaacggt gtaaatactt tcgaatggat
     2401 ttgaatagaa aatcaatgcg gtggtaaaaa acacgtcgta aagtatctgg ctagtcaaaa
     2461 aatagcgaaa tttcgcagct ttttcataga aaagaattaa tgcgctagtt agggcaaagg
     2521 tagccccaat tagaatgtaa ctgcgattta gaacatttct ttcgataacg ccgataattt
     2581 gcaaccaaat cagagtagtc aaaactgaga aaagcatcaa gagcctgaga aacatgtaca
     2641 ggcgaatgtc tttatcggcc gtcaattgcg gttggggcaa attcggtacg ccgtgctgca
     2701 gatattcggc gtaaacacgg tatgccctca ttaattgccc gccattgtaa agattggcag
     2761 atacatcgcg atcaacattc ccccaaccat gcccccgagg aacacgagaa tcagtggttc
     2821 aatcatttta gtcataccgg aaactaaatt ttctacgtcc tcttcgtaat agtctgcaac
     2881 gcgctcaagc attttatcca tgttacctgt gccttcaccg acggtaacca tttgcacaac
     2941 catgcttgga aatattggca gtcggcccat aactgacgac agcgttttgc cattctcgac
     3001 ttccgctctg actttaacta aattttcttc tacggtcgaa tttcctacag ccgttctaca
     3061 tatatcgatc gcatctaata aatttatgcc actcgccaaa agagtctgca tggttcttgc
     3121 aaatcttgct accgatactt taagtaccag ctctccaaac agaggagctt ttaatacgat
     3181 tagatcaaaa aatttttttc cttcttctgt ttttaaataa cttctaaaaa aataaaaaac
     3241 agcgactcca ccaccaacaa taaagagaag atttttttga aaaaaatggc ttgcgtcgat
     3301 aacgaattta gtgacacctg gcaattcttg attctgtgat tttaaaagtt cttcgaattt
     3361 tggaattaca aatatcatca ttactatcaa aacaataaaa cctactgctg tcacaacaat
     3421 ggggtaaacc atcgctcctt ttactaattt tttcagtttt tctgcgtcgt caaggtattt
     3481 gataagtcgt tttaaaattg tatcgagact accagaagac tccccagcct ttaccatgtt
     3541 tataaatata tctggaaaag tgtctttata ggctcccatt gcttgccaga gaaatgaacc
     3601 accagatatt ttttctcgca gtgctcccaa aatttgtttc aattcattat tactggcttg
     3661 tcccgaaatt atgtccaatc cttgaaccaa cggtatacct gcagacagaa gcactgataa
     3721 ttgtcgggta aataagagta cgtccgctcg tttgaccttt cccccaaagc caccaatttt
     3781 tgcgagatca aattctaaag ctgatgcttt cgaaattcgg attgggcgta gttgttggga
     3841 cctaagcatt atgcgcactt ccccttcatt aggagaatcc actgtccctt ttattttttg
     3901 cccttctttg tttactgcga cgtaagtaaa ctgaggcatc tacaccctca ccatacctag
     3961 cattttggcg agctcttccg ggaactggct aagttcaatg gcatgttctt tagttagagc
     4021 accttgctgc accagcctca ctagcgattg gttcatggtc atcatgcccg actcattttg
     4081 accggcctgc atggtcgaat agatttggtg taacttatct tctcgaataa gattgcgaat
     4141 cgcactgttg ggaaacatgg tttccattga aagcacgcga ccagtcccat tcgactttgg
     4201 aatcaactgc tgggacacaa tgccttgaag tacaaatgcc agtagctgcc taacttgcgg
     4261 ttgttggtgc ggcgtaaata cgttcactat acgataaata gtctgtaccg cattgttggt
     4321 gtgaagcgta ccgaacacca agtgaccagt ttctgcagca gtaagggcca tttcgattgt
     4381 ttcgatatcg cgtaactcac caactaaaac gaagtcaggg tcttggcgca aaacccgttt
     4441 caacgcatct ttgaaagttt tagtgtcggt tccgacttct cgttgattta cgatacaaga
     4501 tttatgcggg tgaataaatt cgattggatc ttctatagtt attaagtgcc cagactcggt
     4561 ttgattaagg aggtcgagca tcgcggccaa agtggtactt tttccgctgc ctgtcggacc
     4621 cgtgactaat accaatccgt ttggacgctt tagtatggtg tctctctggc agtctgggtc
     4681 tgtggcaatc aaagcgattc gtggcattac
//
LOCUS       DAOMXO010000094         4695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9774_length_4695_cov_4.698276, whole genome shotgun sequence.
ACCESSION   DAOMXO010000094 DAOMXO010000000
VERSION     DAOMXO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9774_length_4695_cov_4.698276"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLH57_03055"
     CDS             <1..261
                     /locus_tag="PLH57_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01618.1"
                     /translation="HWSWCARQALNSGIFDCLVLGGGTRDALELRRLQLLAEKSKCPV
                     FLLSNEPTQAGAWPIQMQFSVQRMFDGSLSVRKLSLKKHSLG"
     gene            268..1632
                     /locus_tag="PLH57_03060"
     CDS             268..1632
                     /locus_tag="PLH57_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01619.1"
                     /translation="MVLCFFLEQKTNTSSKRLRLFAEACYRYTPRIAVRENEAVFLEI
                     GKSLHLFQGQSLPLRLQALADKFQIKGRVVSGHCVLASWIRALVGQSEKRVETLAIDI
                     LPICDDVLEQKPEDRDERERKILNFVGYLKKLGIKNVGDFFKIATTSLGSRFGQIAGE
                     LQQELLKAAGRSQAPDFWPIFRLSERIQEQQDLSDPSARRGIEGLEPLLFMMRAMVER
                     LVTRVRARSEKIASLRVEFEQEWSLKKSEKKRRIDLLFSVAQSASIGILTILKNRFER
                     EFNSRPLESEILSVEVTVLESVPGPVAQAHLFDRDQEETEKMETFYVRVQERLGCDHI
                     FFAETSENYLPEKSWKKTGPIEKNNFLNSETSLVRPTRLLKRAVPVLRRGDLFFLKNK
                     TAHHWKIIDVNGPERISGYWWETSAQEPKMVCRDYYRFVTQSGEHLWVFNEGGSFFLH
                     GFFD"
     gene            1638..4679
                     /locus_tag="PLH57_03065"
     CDS             1638..4679
                     /locus_tag="PLH57_03065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HRK01620.1"
                     /translation="MRYSELLCKTNYSFLRGASHPEECVAQAAVQGLYAIGICDRNGV
                     YGIPKAYQESKKHPKLKLLVGAELLCEKRAPVQFLARTRAAYGLLCRVITTAHAGKEK
                     GQAVLNWSELLALCDAFSAGAGDLLVFSDTEEGAGDLKDIFGANFYLPLFRGLDGKDK
                     YRTERAKRIVARFGIQIVATNDVHYHVQSRSVLQDVVAGIRDSVPLDSAGKKLFSNAE
                     RYLKTPAQMAKLFSDMPDAVERTAEIADQFSFSMAELRFRYPSEWIPAGQTAQHYLTT
                     LVWTGACSRYHGNIPVEVERQIAHELQLVEQLQFADYFLTIWDIVEFARGRKILCQGR
                     GSAANSILCYCLGITAIDPVRMNLLFERFISVERGEPPDIDVDFEHERREEVLQYVYE
                     KYGRHRAAMVAAVITYRTRSSLREVSKTLGLDLDRVKSEEDDFPKKQLARQLAQELKG
                     FPRHLSIHSGGFTLSQDPIIETVPVEPARMEGRTIVQWDKYDLDIVGLLKVDLLSLGM
                     LSAIQKTCELVGLEYSKIPAEDPATYQMIQRADTVGVFQIESRAQMNMLGRLQPESFY
                     DLVIEVAIVRPGPIVGRMVHPYLRRRRGLENPKSPDPRLEPILGRTLGVPLFQEQVMK
                     MAIVMAGFTPGEADELRRAIGAWRSSGSIEKMGQKLRDGFLKNGLPSEFVDRVFEQIK
                     GFAEYGFPESHAASFALIAYASSYLKCHYPAEFLCSLLNSLPMGFYSEHTLVEDAKRH
                     GVKVLPIDVNGSDWDCRIESGELRLGFRLVKGMARKVFDRLFNERSERPFAGIGDFIA
                     RAKPSLRILESLALGGVFEPFKVSEREALWEALAYDCLLRAELGQSQGQQLSLFSQSG
                     LSGLLREQSAPQLVGLDDFERVSAQFHSFRLSLGEHPMPVLRRFLRQVPRAVTADAKK
                     GRHGQRFRVAGMVIVRQRPPTAKGTVFSALEDETGLLDLIFHKAVHEKYQKTLSSNAF
                     LVASGELQKDGYSVSLLVSKIEPLMPPETEQTLNFKTHDWH"
BASE COUNT         1178 a          982 c         1260 g         1275 t
ORIGIN      
        1 cactggagct ggtgtgcaag gcaggcgcta aatagcggca tttttgattg tttggtttta
       61 ggggggggca ctcgcgacgc gctagaactg cggcggttgc agctcttagc tgaaaaatcg
      121 aaatgcccag tttttctttt gtcgaatgaa cccacacagg cgggtgcttg gccgattcaa
      181 atgcagtttt cagttcaacg aatgtttgac ggatcactca gcgttcgtaa gttgagttta
      241 aaaaaacatt ccctagggtg agtcgccatg gtgctttgtt tttttctcga acaaaagacg
      301 aatacgtcta gtaaacgctt acgtttattt gctgaggcct gttatcgtta cacgccgcgc
      361 attgcggttc gtgaaaatga agccgttttt ttagagattg gaaaatctct tcatcttttt
      421 cagggccaaa gtctgcctct tcgattacaa gcgcttgcgg acaagttcca aataaaggga
      481 cgtgttgtaa gtggacactg tgtgttggca agttggattc gagcgttggt gggtcagagt
      541 gaaaaaagag tagagacgct ggcgatcgat atacttccta tttgtgatga cgtcttagag
      601 cagaaaccag aagatcgcga cgagagagaa agaaaaatat taaatttcgt cggatatctt
      661 aaaaagctcg gcattaagaa tgtaggggat ttttttaaga tagctaccac ctctttggga
      721 tcacgttttg gtcagatagc aggtgagtta cagcaagaac ttttaaaggc cgctggtcgt
      781 agtcaggcgc cagatttttg gccgattttc cgattgagtg aacgcattca agaacagcaa
      841 gacttatcag accctagtgc aagacgtgga atagagggct tggagcccct gttgttcatg
      901 atgcgagcca tggtggaacg gttagtcacg cgcgtacgtg cacgatctga aaaaatagcc
      961 tcgcttcgag ttgaatttga acaggagtgg tctcttaaga aaagtgaaaa aaaacgtcga
     1021 atagatttgt tattttccgt ggctcaaagc gcttcgattg gcattttaac catacttaaa
     1081 aatcgatttg aacgcgaatt taacagtagg ccactggaat ctgaaatttt gagtgtcgaa
     1141 gtcacggtct tagaatcagt gccaggtccc gtagcgcagg cacatctttt tgatcgtgat
     1201 caggaagaaa ctgaaaaaat ggaaacgttc tacgttcgtg tacaggaacg tttggggtgc
     1261 gatcatattt tttttgccga aacttcggag aattatttgc cggaaaaatc ttggaaaaaa
     1321 actggtccca ttgagaaaaa taattttctc aattccgaaa cttcactggt acgtcccacc
     1381 cgactgctca aaagggccgt tcctgtttta cgacgaggcg atcttttttt tcttaagaac
     1441 aaaaccgcgc atcattggaa aattatcgac gtcaatggac cggagcgcat tagtggttat
     1501 tggtgggaga cctcagcaca ggaacctaaa atggtttgcc gggattacta tcgtttcgtg
     1561 actcaatccg gagaacacct ctgggttttt aacgaaggtg gaagcttttt tctgcacgga
     1621 ttttttgatt gagtgccatg cgctattctg agcttctttg taaaaccaat tactcttttt
     1681 taagaggggc gtcgcatcct gaagagtgtg ttgctcaggc agcagtacag gggctttatg
     1741 cgatcggcat atgcgatcgc aacggtgtgt acggaatacc gaaggcctac caggagtcca
     1801 aaaaacatcc caaacttaaa ttgctggtgg gagcggagtt gctttgtgag aaacgggcgc
     1861 cagtacagtt tttggcccgc actcgcgcag cctacgggct gctctgtcgt gtgatcacca
     1921 cggcccatgc tggtaaagaa aaaggacagg ctgttctgaa ctggtcagag ttgttggcgc
     1981 tttgtgacgc gttcagcgct ggtgcaggtg atttactggt tttttccgat accgaagagg
     2041 gggctggcga tctaaaagat atttttggtg ccaattttta tttgccactt tttcgtggtt
     2101 tggatgggaa agataaatat aggactgaac gagcgaaacg gattgttgcg agatttggca
     2161 ttcaaattgt ggccactaac gacgtgcatt accatgtgca gtcccgttcg gtattacaag
     2221 acgttgtcgc aggaatacgg gacagtgtac ctctcgattc agccgggaaa aaattattct
     2281 ccaatgctga gcgctattta aaaacgcccg cccaaatggc caaattattc tcagacatgc
     2341 cagatgcggt cgagcgcact gcagaaatcg cagatcagtt ttctttttcg atggctgaat
     2401 tgcgttttag gtatccatca gaatggattc cggcggggca gaccgcgcaa cattatttaa
     2461 cgactctcgt ttggacgggg gcgtgctcgc gttatcacgg aaatattcca gtcgaggttg
     2521 aacgtcagat cgcacacgaa cttcagttgg tcgagcagtt gcagtttgcc gactattttt
     2581 tgacgatctg ggacatcgta gagttcgcac gcggtaggaa aattctctgc caaggtcggg
     2641 gatctgccgc caattcgatt ctttgttatt gtctgggaat cacagcgata gacccggtga
     2701 gaatgaatct gctgtttgag cgatttattt cggtcgagcg cggagaaccg ccagacattg
     2761 acgtcgactt tgagcacgaa cgtcgtgaag aagtgcttca gtatgtatat gaaaagtatg
     2821 gtcgtcaccg cgctgctatg gtggccgccg ttattactta tcgaacccgg tcgtcattgc
     2881 gcgaggtttc taagaccttg ggactcgatt tagatcgtgt gaaatccgaa gaagatgatt
     2941 ttccaaaaaa acaattagca cggcaactag ctcaggaatt gaaggggttt ccgagacacc
     3001 tttctattca ctctggtggt ttcactttat ctcaagatcc gatcattgaa acagtaccgg
     3061 ttgaaccggc tcgaatggag gggcgtacca tagtgcagtg ggacaagtac gacctcgaca
     3121 ttgttgggtt gttgaaggtc gatctcttaa gtcttgggat gttgagcgcc attcaaaaaa
     3181 cttgtgaact agtgggacta gaatattcca agattcccgc tgaagacccg gcaacctacc
     3241 aaatgattca acgggcggat acggtgggcg tatttcaaat cgagtcacga gcgcaaatga
     3301 acatgctcgg acgcctgcaa cccgagagtt tttacgacct cgtgatcgag gtggcgattg
     3361 tgcggccggg ccccattgtg ggccgcatgg tgcaccctta tttacggcgt cgtcgaggac
     3421 ttgaaaaccc aaaaagtcca gatcctaggc tcgaaccaat tcttggtcgt actctggggg
     3481 tgccgctttt tcaggaacaa gtcatgaaaa tggctatcgt catggcggga tttactcccg
     3541 gcgaggctga tgagctacgg cgcgcgatcg gtgcctggcg ttcgagtggg tcgattgaaa
     3601 aaatgggcca aaagctgcga gatggttttc ttaaaaacgg gttgccctct gaatttgtgg
     3661 atcgtgtatt tgagcaaata aaaggttttg ctgagtatgg attcccggag tctcacgccg
     3721 cttcgttcgc attaattgct tatgcgtcgt cgtaccttaa atgtcattat cccgccgagt
     3781 tcctgtgctc cctgttgaac tccctgccaa tggggtttta ttccgagcac acgttggtgg
     3841 aagatgccaa aagacatggc gtaaaggtat tgccgatcga cgttaacggt tcagactggg
     3901 attgccgcat tgaaagcggc gaactgcgtt taggatttcg tttagtaaag ggaatggcgc
     3961 gcaaagtgtt cgatcgactt tttaatgaac gaagtgaacg gccgttcgca ggaattggtg
     4021 atttcattgc gcgggccaag ccatctttgc gaattttaga aagtttggcg ttgggtggcg
     4081 tttttgaacc atttaaagtg tccgaacgcg aagccctatg ggaggcgttg gcctacgatt
     4141 gcttgctacg agctgagctt ggccagagtc aggggcagca acttagtctg tttagtcaga
     4201 gcgggctttc gggtttgcta cgcgaacaga gtgcgcccca gttggttggg cttgatgact
     4261 ttgaacgtgt gagtgcccag tttcattctt ttcgattgtc gttgggtgaa catccaatgc
     4321 cagtattgcg acgttttctt cgtcaggttc cacgcgcggt tacggccgac gccaaaaagg
     4381 ggcgacatgg tcagaggttt cgagttgctg gtatggtgat tgtgcgtcag cgcccgccta
     4441 ccgctaaggg cacggtcttt agtgccctcg aagatgaaac gggtcttctg gacttaattt
     4501 ttcacaaagc agtccatgaa aaatatcaaa aaacgctgag ttctaacgca tttttagtgg
     4561 cttcgggcga actgcagaaa gacggttatt ccgtaagtct gttggtgagt aaaatagaac
     4621 ctctaatgcc accagagact gagcaaaccc tgaattttaa aacacatgac tggcattaaa
     4681 cggcgagccg agata
//
LOCUS       DAOMXO010000095         4618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9983_length_4618_cov_4.373877, whole genome shotgun sequence.
ACCESSION   DAOMXO010000095 DAOMXO010000000
VERSION     DAOMXO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9983_length_4618_cov_4.373877"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PLH57_03070"
     CDS             <1..1164
                     /locus_tag="PLH57_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01621.1"
                     /translation="DPCNMAKGFEKALFPELVSEISYGMAAALCTADCAFTLAGSSGD
                     ACKTATLLVGAVDLAAAAWFAAESDEMLKKLGGLATQATVVVGAGKAGAGWRTTACLT
                     GLTNAVQAMARHKARSDFRESIRDLHAQKWCLMAGGLNLNSLTASQEFVAKSAGATGG
                     INGDVSMSGDGYLSPENAARETDKIFSRMTAQQQSFFDGSFNRDGINGKELMANVLSG
                     KAKNGYEAALHALGAGKLGDKGALAAKEISGLANHLAATRKKELAEADRKYNPNSTAA
                     MFASAGGGTRAPAKEANPLAGFGDLLTQMMPGGQKKADLDRSGTAGGVNFGTRGPAAA
                     ALAQREGLHTSDQSLFDVVGSRYQAISRRFMSEGDQPEYAPSAGLPENPYLTR"
     gene            1255..2481
                     /locus_tag="PLH57_03075"
     CDS             1255..2481
                     /locus_tag="PLH57_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01622.1"
                     /translation="MLKQISILTTVAMLAPALAFAASSLIQDERWDTLVDPSIDYVAT
                     YTPIEWVVGGAYRKSPKIPAGIKIDRSNNNLALGYHEGRLYFAWRTAPIHFASEKALM
                     HIVSSRDFGQSWVHEHTIRLGRDVREPFFISYKGDLYFYYLELGKNFRKFEPRQTWVI
                     RRQGEKQWSRPKPWGKPGEIAWEFKVRRDQVWVSSYTGNHYSTNDSKMEVELKVSSDG
                     QTWTPHDLPVYRGGVSEVGFEFDEENNLWAVTRNEDGDSSGFGSHVAFADIDRYWRWQ
                     IPAKSNPRRYDSPRMFRHGDDLYLIARRDVGGVFGNGWQNKLVRWYKYWRRPKRTALY
                     RIDRQKREVVHLFDFPSAGDTSFPSILRTGPHSYLIANYTSPLKFPGRSWMWGQVSPQ
                     GTQIYFTTLHFAPKNK"
     gene            2551..3045
                     /locus_tag="PLH57_03080"
     CDS             2551..3045
                     /locus_tag="PLH57_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01623.1"
                     /translation="MRLLTLSGLFLVAWTLLLDSPAKADFPLEDKLVLADAPSYPPRG
                     SREKVYRQGHLTITQYAWTEAVDITTDMVRMLVRVKDDNTNREYTFHPDIVQNNIPAE
                     TQFQFVGVEWTSRGTVLAPVVYYKNERGETYQLSDIRFFEKPRSAEPQPPQPIRDRTC
                     NETI"
     gene            3108..3183
                     /locus_tag="PLH57_03085"
     tRNA            3108..3183
                     /locus_tag="PLH57_03085"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3141..3143,aa:Ala,seq:ggc)
     gene            complement(3569..3886)
                     /locus_tag="PLH57_03090"
     CDS             complement(3569..3886)
                     /locus_tag="PLH57_03090"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK01624.1"
                     /translation="MKKTKGVSLETVIERQIKKDKDFRILFDERRFYLQVAHLITNLR
                     AKSGLSQSEVAKAAGVSQPLIARLEKGDQRRAPTFDTIYRILKTLGYQLEISVTRDHR
                     KAA"
     gene            complement(3883..4215)
                     /locus_tag="PLH57_03095"
     CDS             complement(3883..4215)
                     /locus_tag="PLH57_03095"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRK01625.1"
                     /translation="MKISFFESRSGRSYFEDFLDDLHRNDRAAILAVFADIQEHGLNA
                     IGCQFRQLEGKLWEIKIRTAGGGWRFFYVMLAPDHMHVLHSYKKQGQKAPKHELEVAR
                     KRLKETSR"
BASE COUNT         1122 a         1258 c         1161 g         1077 t
ORIGIN      
        1 gacccctgca acatggcaaa aggttttgaa aaagcgctct ttccggaact tgtttctgaa
       61 atcagctacg gcatggccgc agcactgtgc accgctgact gcgcgtttac gcttgccggc
      121 agcagtggag acgcctgcaa aaccgctact ctcctggtag gcgcggtgga tttggccgcc
      181 gctgcctggt tcgccgcaga aagcgatgag atgctcaaaa aattaggcgg cctcgccacc
      241 caagctactg tcgtagtagg tgcaggaaag gccggcgccg gctggaggac caccgcctgc
      301 ctcactggac tcaccaacgc ggttcaagct atggcgcgcc ataaggctcg ctccgatttt
      361 cgggaaagca ttcgagatct tcacgcccaa aagtggtgtt tgatggcggg cggacttaat
      421 ttgaatagtc ttactgctag tcaagagttc gtcgccaaat cagccggtgc aactggcggc
      481 ataaacggcg acgtttcgat gagtggcgac ggttatcttt cgcccgaaaa cgccgcgagg
      541 gaaaccgata aaattttttc acgcatgacg gctcagcaac aatctttttt cgatggttcg
      601 tttaatcgcg acggaatcaa cgggaaagaa ctcatggcca atgtgctgtc cggtaaggca
      661 aagaatggct acgaagctgc cctgcacgca cttggtgcag gcaaactggg cgacaaaggc
      721 gctttggctg ctaaagaaat ttccgggctg gccaatcatc tagcagcgac tcgcaaaaag
      781 gaactggccg aggctgaccg aaagtacaac cccaactcta ccgctgcgat gtttgcttcc
      841 gctggcggtg gcacccgcgc tccagccaaa gaagccaatc cacttgctgg tttcggcgat
      901 ttgcttacgc agatgatgcc aggcgggcaa aagaaagccg acctcgaccg ctcgggtacg
      961 gctggtggcg tgaacttcgg cacccgcggc ccagccgcag cagctctcgc ccaacgagag
     1021 gggctccaca cttcggacca gtcgcttttt gacgttgtgg gttctcggta tcaggcaatt
     1081 tcacggcgtt tcatgagcga aggcgatcaa cctgagtacg cacccagtgc agggcttcct
     1141 gaaaacccct acctcactcg ataaagcgcg ggtttcattt tagtttatgc cccctctcta
     1201 ggcagcaatg acctactctt ttcagccgtg cgttttagcc tcagactaga atgtatgttg
     1261 aaacaaatct ctatcctaac cacagtagca atgcttgcgc cggcgctagc cttcgctgcc
     1321 agtagcctga tccaagacga acgctgggac acacttgttg acccttcgat cgactacgtg
     1381 gcgacctaca cgccaatcga atgggtggtc gggggtgcct accgcaagtc tccgaagatt
     1441 cccgccggta tcaagattga tcggtctaac aacaaccttg ccctcgggta ccatgagggg
     1501 cgattgtatt tcgcctggcg tacagcccct atacatttcg catcagaaaa ggccctcatg
     1561 cacatagtat cgtcgcgcga ctttggccag agctgggttc acgagcacac cattcgactc
     1621 gggcgcgacg tacgtgaacc attttttatt tcgtacaaag gtgatttgta cttctactac
     1681 ctagagctcg gaaaaaactt tcgtaaattt gaacctcgcc aaacctgggt cattcgtcgc
     1741 caaggcgaaa aacaatggtc tcgccccaaa ccctggggca aacctggcga aatcgcctgg
     1801 gagtttaaag ttcgccgcga ccaagtttgg gtgtcgtcct acacgggcaa tcactacagt
     1861 accaacgaca gtaaaatgga agtagagctt aaagtctcta gcgacgggca gacctggact
     1921 ccgcacgatt taccggtgta ccgggggggc gtatccgaag taggctttga gttcgacgaa
     1981 gaaaacaacc tttgggcagt gactcgcaac gaagacggcg actcgtcggg gttcggttcg
     2041 cacgtagcct tcgccgatat cgaccgctac tggcgctggc agatacctgc gaagtccaat
     2101 cctcggcgct acgattcacc tcggatgttt cgccacggcg acgatttata tttgatcgca
     2161 cgtcgcgatg tggggggcgt tttcggcaat ggttggcaaa ataaactcgt gcgctggtac
     2221 aagtactggc ggcgccccaa acgcacggcc ctttatagga tagatcgcca aaaacgcgaa
     2281 gtggttcacc ttttcgattt tccctcggcg ggcgacacca gttttccatc aatcttacga
     2341 accggccctc atagctacct aatcgccaat tacacttcgc cgctaaagtt ccctgggcgt
     2401 agctggatgt ggggccaagt aagcccgcag ggtacccaga tctattttac gacccttcat
     2461 ttcgcgccaa aaaataagta attcgccgct tgcattaacc gactgtttta gttaaaaggt
     2521 cgccacttag caaaaaaaag gtggtgcacg atgcgtttgc tcacgctctc tggcctgttc
     2581 cttgttgctt ggactcttct tctcgactcc cctgcaaaag ctgattttcc attggaagac
     2641 aagctcgtct tggctgacgc gccgtcttat ccgccacgcg gctcgcgtga aaaggtgtat
     2701 cgccaaggcc acttgacgat cactcagtac gcctggaccg aagcggtaga tatcacgaca
     2761 gacatggttc gcatgttggt gcgagtgaaa gacgacaaca ccaatcgcga atacactttt
     2821 caccccgaca ttgtgcaaaa caacattccc gccgaaacac aatttcaatt tgttggcgta
     2881 gaatggacga gccgcggaac cgttttagca cccgtcgtgt attacaaaaa cgagcgaggg
     2941 gaaacttacc agctgtctga tatccggttt tttgagaaac cacgttctgc ggaaccccaa
     3001 ccgccacaac ccattcgtga tcgtacttgc aacgaaacga tctagttgcg tttttgaaaa
     3061 gctggcgtta acttgcggtt tctttgacaa ttcgttcgtt aatgaatggg gctgtagctc
     3121 agctgggaga gcaccacaat ggcattgtgg gggtcgtcgg ttcaatcccg atcagctcca
     3181 ccaattccgc cgcaggcgga ttggtggttt gtaaaaagga ggcaggatgc cgaccttcct
     3241 taactgagta gcgagcgcag gaaagcacga gccatcagct ccaccacttc tcgcgcgagg
     3301 cgcgaaagtg gtgctataaa agtgctggca ggatgccagc caaccatggc tgagtagcga
     3361 atgccaggag gccgaagcca tcagctccac catttttcga gttcggattt gatcgttttc
     3421 agagcaagta tcaaaaatca aaagcgaaag gttaagcggg tttagcgaga ccgcttccaa
     3481 gagaacatca cagggtcacc cccgaaatca cgggggtggt cttagttttg tcttaccaca
     3541 cacgagtagg ctaaaaacca tactcgactt acgctgcttt tcgatgatcg cgtgtcacac
     3601 tgatctcgag ttgatacccg agcgtcttta gaatccgata aatcgtatca aaggtcggag
     3661 cacggcgttg atcacctttt tctagtcgtg caatgagggg ttggcttact cccgcagctt
     3721 tagccacctc actttgggaa agaccagact ttgctcgaag attggtgatg aggtgagcta
     3781 cttgtaaata aaacctgcgc tcatcgaaaa gaattcgaaa atccttatcc ttcttaatct
     3841 gtctttcaat cacagtctca agcgagacgc ctttcgtttt tttcatcttg atgtctcctt
     3901 caatcgcttt ctcgcgacct caagctcgtg cttcggggcc ttttggcctt gtttcttata
     3961 cgaatgcagc acatgcatat gatccggggc cagcataacg taaaagaacc gccagccgcc
     4021 tccggctgtc cgaatcttaa tctcccaaag tttgccctcg agctgcctaa actggcagcc
     4081 gattgcattt agtccgtgct cctggatatc agcaaatacc gccaagatgg ctgcccggtc
     4141 attccggtgc agatcatcca gaaaatcttc aaagtagctg cgtcccgacc gggactcaaa
     4201 gaagctgatt ttcaccaaac tattataacc aaatggttat gataccgcaa gatccgtttt
     4261 tgctttgtct taattctgtc ttactgagga gttaagtctt cgaaatttaa gagggtgcgc
     4321 cgaatccacc acttctcgcg cgaggcgcga aagtggtgct ataaaagtgc tggcaggatg
     4381 ccagccaact atggctgagt agcgaaggcc aggaggtcga agccatcagc tccaccaatg
     4441 tttaattgtt agcgccatta tccaacgaga cgctctctct tcctacccac cagcaacaac
     4501 ttcaccggca aggcaccgtt tctcagcgcg actttttgat tcaccgaaag tcccaaccaa
     4561 gcatcaggta tttcggtagg taacagcaaa gccacacgcc aaccgcgaaa acgatttt
//
LOCUS       DAOMXO010000096         4616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_9991_length_4616_cov_4.639114, whole genome shotgun sequence.
ACCESSION   DAOMXO010000096 DAOMXO010000000
VERSION     DAOMXO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9991_length_4616_cov_4.639114"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..4070
                     /locus_tag="PLH57_03100"
     CDS             252..4070
                     /locus_tag="PLH57_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK01626.1"
                     /translation="MSQLFYPLLCAFNLITFATLTNLVAPASQVFAAQSGETLEAPFH
                     KPGHFSIGANKTFTIHIHGDELAVEGFKLVHFHKGNEAGYVLTIGLPENSDGDIDVYR
                     VTNFAPADAILKYELFSIGQATDPSIGAMFFARSSDNPLSSFAFSLQKLTDGKLVAKS
                     LPSSDISFKSLGKLPIDSYAFIPTPQFVDPREIVLNAGLMVVHSPLRNTFYPVPVRIS
                     RHGDYLSVDLGKLTFFGELWGPGETRSVDLLARTTRIRSIGEKTFELQWLDQNDQTRS
                     VTLWQNHYPESKQATHNSPYSPRTGEVAWETGAQAILRFLNSFPVYGLEARAVKSSAP
                     LRPYSFAGLSDPFGSWVRQGRWEHVVGSFSLPNIFQNLVSMVSDFINPSESDSYDSAH
                     KSGSRRNELLVLSIPNQLIDLNPNTSSELIAQLIEAHSKLDAHHPFESEIVYIRIEDI
                     TEFNLPSIKSLLNSKLGATLSLRLSENSLPEAIVVIIELVPNATQTKLDLNHLTKLID
                     HLEEWPKTLREHGILTPLRIALVSDDRTLPSNTLEQSLAFTEALRRVRGTTPQSAAEP
                     RDRALGQIRLSELSSTVFEYFRKKLNQTPQLVDVWPLLSRNSRLQHLSELIETSVALR
                     LSAAEIDEILIRAEHLLPRLEQRLDAYNGISSVFLEALKFAKRNSNNDSAILFAEAAR
                     ILAASLGGFTEPRFEVEDLKRIEALGRKLNASSTTASPINFIGRERQIEAVKQNLLSA
                     ARGGFKDKATNVIVFFGESGTGKTELAEAIARETEAERRYIDLRNYDAMGENSLQKAA
                     IALQASSRAQKILILDELDKSKSETVRNTLAATLGNRDGKDLEINLKGTTVIMILNID
                     VRNESYRQMLDAYKRNEPVKPYFVEVIRNALLYGDDSNDTQLEFRDADSFVKRFVNDA
                     IFMPPISADIGQQEQLIDAKVRAYEREKGVRIVVPNKTLQFLIARAVENSDGNFRSVE
                     RAIPSFFRAAEAEYVIRNPNDEALTASKYYQLKPVESREGDSHLTLELAPLRLDDRIG
                     VVTMLRDQQHRFYDRLIRHFREKEELFRTAKNQTPVKAQSDVLNDIANTYAGVQSELK
                     ALRSQVERTLIARPTPAISEFQAGEGNILFGSYFKLFPAALGLFDGDELQATELVGHE
                     IDKGIITLSKLIATPDIAHAGTRAWYDTYLEKSGIDRNAPLPIRVSRVYIRKLGEVFA
                     HDSWYLIDRMVDEGKKYSEEIRKRADQSNTRIELPAALAQCNELIKTRQSIAAAIEAQ
                     ISKLPKGKSQ"
     gene            4067..>4616
                     /locus_tag="PLH57_03105"
     CDS             4067..>4616
                     /locus_tag="PLH57_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01627.1"
                     /translation="MRWFLFSCWLVLLAPVGFAPRAAANEEHVAIAESQFRPIGRLKV
                     ESDGTVVLVLSESERVRLQNFSVKADPSISEKQPRWSLCIAHPSAERSTTQHPNPLFY
                     YHSIEELQETRVYDLGLYGSPDNQNPTAARLFFLHSGDREVFAIALDPNTGQGVVKMK
                     IPFASHGSLKMSQPLSGYAVLPT"
BASE COUNT         1207 a         1224 c         1042 g         1143 t
ORIGIN      
        1 gtgcccaatc gtaggacgtc gtcgcagtcg ttgtcggcag cccaaagggg cgcaccaaac
       61 agcgcgaggc tgattgccag ataactcttc acggccaacc ccgtgaactt cttgaaattc
      121 atggcgagcc cttaccaatt ccgacagttt aaatcaagat ataagagcta taggaaatgg
      181 gtctaattca ttgttttttc agactaatta aattgatcat ttttatttga tcgtgttaga
      241 cacggcgcga tatgtcgcag ctgttttatc cccttttatg cgcctttaac ctaattactt
      301 ttgcgacttt aactaacctt gttgcgcctg cttctcaggt gttcgctgcc caaagcggcg
      361 aaactctgga agcgcccttt cacaagcctg gccatttttc tatcggcgca aacaaaactt
      421 ttacgattca catccacggc gatgaactcg ccgtagaagg gttcaaacta gttcactttc
      481 ataagggaaa cgaagccgga tacgtactaa ctatcggact cccggaaaac agcgacggcg
      541 atatcgacgt ttaccgagtg actaacttcg cgccggccga cgccatacta aagtatgagc
      601 tcttttcaat tggtcaggcc accgaccctt cgatcggagc gatgtttttt gcgcgctcca
      661 gcgataatcc gttaagtagt tttgcttttt ccctacaaaa actgactgat ggtaaactag
      721 tcgcgaaatc gctaccttca tcggacattt cgtttaaaag cctcggcaaa ctaccaatcg
      781 actcgtacgc ttttattccg actccgcaat ttgttgatcc acgagaaatc gtactcaatg
      841 ctgggttgat ggtggttcac tcgccgctgc ggaacacctt ttacccagtt cctgttcgca
      901 tttcccggca cggtgattat ttgtcggttg atctcggaaa gctcactttc tttggcgaac
      961 tttggggccc cggtgaaacg cgttcagttg atttgctcgc ccgcacgact cgaattcgct
     1021 cgattggcga aaagacgttt gagcttcaat ggctcgacca gaacgatcaa acacgttccg
     1081 taaccttatg gcaaaaccat taccccgaat caaagcaagc aacccacaac agcccttatt
     1141 cgccgcgaac tggggaagta gcttgggaaa ctggggctca agcaatcctt aggtttctaa
     1201 atagttttcc cgtttatggt ctcgaggctc gcgcggtgaa gtcctcagct cctctaagac
     1261 cctattcatt tgcgggtctt tcggacccct tcgggtcctg ggtacgccaa ggccgctggg
     1321 aacatgtcgt tggaagtttc agtttgccaa atatttttca aaatctggtt agcatggtta
     1381 gcgattttat caacccgagc gagtccgaca gctacgactc tgcacataag agcggttcca
     1441 ggcgtaatga acttttagtg ctatcgatcc ccaatcagtt gatagacttg aacccaaata
     1501 ctagttcaga attgattgct caactgatag aggcccactc aaaactcgac gctcaccacc
     1561 ccttcgaatc tgaaattgta tacatccgta tcgaagacat tacggaattc aatcttccta
     1621 gtatcaaaag tcttctaaat tctaaactag gagcaacttt gtcgctgcga ctctccgaga
     1681 actctttgcc agaagccatc gtggtgatca tcgagttggt cccaaacgcg actcagacta
     1741 agcttgactt gaaccattta accaaactca tcgaccacct cgaagaatgg cccaaaacac
     1801 ttcgggagca cggaatttta accccgctgc gtatcgcgct agtcagcgat gaccgcacgc
     1861 tacccagcaa caccctggag cagtcactcg ccttcactga ggccctcagg cgagttcgag
     1921 gcaccacgcc acaaagcgcc gccgagcctc gcgatcgagc gctcggtcaa atccgcttga
     1981 gtgaactctc gagtactgtt ttcgaatatt ttcgaaaaaa attaaatcaa acacctcaat
     2041 tggtagatgt ttggcctctg ctttctcgca attcgcgact tcagcattta tccgagctaa
     2101 tcgagacttc ggtggctttg cggttgtccg ctgcagaaat tgacgaaatt ctgattcgtg
     2161 ccgaacacct cttgccgaga ctggaacaac gtctcgacgc ctataatggt atttcttcgg
     2221 tctttctaga ggcgcttaaa tttgccaaac gcaacagcaa caacgattcc gctatcttgt
     2281 ttgccgaagc cgcccgcata ctcgctgctt ctctgggagg gtttaccgaa ccgcgattcg
     2341 aagtcgaaga ccttaaacga atcgaagcgc tgggtcgcaa actcaatgct tcgtcgacca
     2401 ccgcgtcgcc aattaatttt attggtcgcg aacgtcagat cgaagcagta aaacaaaact
     2461 tactctccgc agcacgtggg ggttttaaag acaaagccac caacgtgatt gtatttttcg
     2521 gcgagtctgg aactggtaaa actgaattgg ccgaagcaat cgcccgagaa accgaagctg
     2581 aacgccgcta cattgacctg cgaaactacg atgcaatggg tgaaaacagc ctgcaaaaag
     2641 cagcaatcgc actacaggcc agtagccgcg ctcaaaaaat actaattctc gacgaactag
     2701 ataaatcgaa aagcgagaca gttcgcaaca cccttgctgc cactcttggc aatcgcgacg
     2761 gcaaggacct cgaaataaat ctcaaaggca ctacggttat catgattttg aacatcgatg
     2821 ttcgtaacga gagttaccgc caaatgctcg atgcctacaa acgcaacgaa ccggtcaaac
     2881 cttattttgt ggaagtgatt cgaaatgcgc tgctttacgg tgacgacagc aacgataccc
     2941 aactagagtt tcgcgatgcc gatagtttcg taaaacgatt cgtaaatgat gctatattca
     3001 tgccgccgat ttccgccgat attggccaac aggagcagtt gatcgatgca aaggtccgag
     3061 cctacgagcg tgaaaagggc gtgagaattg tggtgcccaa taaaactctt cagttcctga
     3121 ttgcgcgagc cgtggagaac tctgatggta attttcgctc tgtcgaacgg gcaattccgt
     3181 cattttttcg ggccgccgag gcggaatatg taattcgcaa tcctaacgat gaagccctca
     3241 ctgcttcgaa gtactatcag ctgaaaccgg tggaatctcg cgaaggagat tcccacttaa
     3301 ccctggaact ggctccactt cgactagacg atcgcatcgg cgttgtgact atgttgcggg
     3361 atcaacagca tcgattttat gatcgtctta ttcgccactt tcgcgaaaaa gaagaactgt
     3421 ttcgaacggc taagaatcaa actcctgtta aagcgcaatc tgacgttcta aacgacatcg
     3481 ccaacaccta tgcgggagtc caatcagaat taaaagcact tcgaagccaa gtagagcgta
     3541 cgcttatagc ccgccccacg ccggctattt ccgaatttca agccggggaa ggaaatatac
     3601 tttttggatc ctacttcaaa ctgttccccg cagcgctcgg gcttttcgac ggtgacgaac
     3661 ttcaagctac cgaactcgtc ggtcacgaaa tcgataaagg aataatcacg cttagcaagt
     3721 taatcgcgac ccctgatatt gctcacgcag gcactcgagc ctggtacgat acttaccttg
     3781 aaaaaagtgg catcgatcga aacgcccctc tccccattcg agtttccaga gtttatattc
     3841 gcaaattggg cgaagtgttt gcccatgaca gttggtacct aatcgaccga atggtagacg
     3901 aaggtaaaaa gtactccgaa gaaatacgaa aacgggctga ccagtcaaac acacggattg
     3961 aactaccggc cgcacttgcg cagtgtaacg aactcattaa aactcgccag agcatcgcgg
     4021 ctgcaattga ggctcagatt tcgaaactac caaaggggaa aagccaatga ggtggtttct
     4081 tttctcgtgc tggcttgtcc tccttgcccc cgtggggttt gctcctcggg ccgctgcaaa
     4141 cgaagaacac gttgcaatcg ctgagtctca attccggccg atcggccgtc tgaaagttga
     4201 atccgatgga acggtggtcc tcgttttaag tgaatcggag cgcgtccgac ttcaaaattt
     4261 ttcggttaag gcggatccct cgatttcaga aaaacagcca cgttggtcgc tttgcattgc
     4321 gcacccctca gctgagcgct ccacaacaca gcacccgaac cctttgtttt actatcattc
     4381 gattgaggaa ctgcaggaaa ctcgagtgta tgacttaggc ctgtacggtt cacccgacaa
     4441 tcaaaatcca actgctgcac gcctgttttt cctgcactca ggtgaccgag aagtatttgc
     4501 cattgcactc gatccgaaca ctgggcaggg cgtcgtaaaa atgaaaattc cttttgcctc
     4561 ccatgggtcg ctgaaaatga gtcaaccact ttccggctac gccgtactcc cgaccc
//
LOCUS       DAOMXO010000097         4612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10002_length_4612_cov_4.702655, whole genome shotgun sequence.
ACCESSION   DAOMXO010000097 DAOMXO010000000
VERSION     DAOMXO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10002_length_4612_cov_4.702655"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PLH57_03110"
     CDS             complement(<1..1399)
                     /locus_tag="PLH57_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRK01628.1"
                     /translation="MNGGISMEKTQHSKSASSDTGSTFRVVDYNTTFPGVFGLHFDLP
                     GQKVNKLDDKVMGELDAQIQKLATRSDIKALVIFSDKEGIFIAGADIQMIQGVHDKET
                     GTKLAASGQTVIAKLEKLKFPVIAAVNGACMGGGTELALCCKMIVTSDSPSTKIALPE
                     VNLGILPGFGGTVRLPERIGLQAAMDYILSGKTMDGQKAVKVGLADACVPAQDFKNRV
                     LKIAADVAAGKFRSQKRAVTGTAKLQKTLLEMNPIGRAVLFSQAKKMILSRTRGKYPA
                     PLKILELLNKQAGASRDKKLKLESIAFGELAVTDVSKRLIELFFATEGIKKQSGVPGF
                     KLDPKDKVTRMGLLGAGVMGGGIAQLMAHRNIPVRMKDISYAGLSAGMAASSKIFAGL
                     VKKRKLTKREADIRLGLISPTLDYSGFGAVDLVIEAVLEDMNVKKKVIKDVEPLLAKN
                     TIFATNTSSLSVTEMA"
     gene            complement(1416..2729)
                     /locus_tag="PLH57_03115"
     CDS             complement(1416..2729)
                     /locus_tag="PLH57_03115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRK01629.1"
                     /translation="MSSTEFFNQGPEVWIVDGLRTPFAKSGTALKDMKAVTLGVHTLR
                     DLLYKMSLDPKTVDEVVIGNTGNPEDAANISRIIALESGIPQNVPAYTVHRNCASALE
                     SVTQGVIKIKSGLADIVVVGGTESMSNMPLIYSKPMTNLFGQLMTAKTPAAKLNALTK
                     FRPGFLKPIIAIQEGLTDAVCGLNMGQTAELLAKENGITRDDQDRFAVRSHEKAVAAT
                     ESGRFKKEISPIPLPPKYDKFLEEDVGPRKGQSLEALGKLKPYFDRANGTVTVGNACP
                     ITDGACMLILMRADKAKALGYRPLGKVRSFAWAGLEPERMGLGPAYSSPKALDAAGLQ
                     MKDMGLVELNEAFAAQVIAVQKMSASKKFAQEKLGRSEAVGEIRDEILNVNGGAIALG
                     HPVGTTGARLVLTLAKEMQIRDVQFGLATLCIGGGQGGSLIVEKV"
     gene            complement(2869..4071)
                     /locus_tag="PLH57_03120"
     CDS             complement(2869..4071)
                     /locus_tag="PLH57_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HRK01630.1"
                     /translation="MKRLGFAAIFIVFLGVTFYGIKDVRGELVFEDELAMEGSTNEVA
                     PAAPVVSEPVAAPRIEERRQITRSEAMRRHRLREELKNEDLLTQKLEELRLKDEMKRT
                     DQIMVSGVEKSNAAESSTLVEQRVGSAATDPALASPATGTTPIAGPGASASSVDGTMV
                     AVDAPKSEDDEALEGRVSIMPRVGLSSITGSMYDVESKFATGITIGVDATDHIGFQAG
                     YTFAKYSIGAGNTLMYPNSFNTGYLQKLNMNDNVIDLGVKGNLLGRKSRVRPFVGAGL
                     AYRKGYVNYDDRTLDYIKRVNPYGASDVEISGFSGYLEAGLEFRITKAISLVGMFRYY
                     NIFTSSQTNPLNPYAFIGSNGYNGGLYYGGLGYNGYGYNQDSKSSAGDALAKNNFYQL
                     SAGVSISF"
BASE COUNT         1145 a         1229 c         1004 g         1234 t
ORIGIN      
        1 tagccatttc ggtaacggat aacgaagacg tgttggttgc aaaaatcgta ttcttggcca
       61 acaagggctc tacgtctttt attactttct ttttcacgtt catatcttca agaacagctt
      121 caatcacaag gtcaacagct ccgaacccag aatagtcgag cgtcggacta atcagaccta
      181 gtcgaatatc ggcctctcgc ttggtgagtt ttcgtttttt caccaagcca gcgaaaatct
      241 tcgaactcgc cgccattccg gccgacaacc cggcataact aatatctttc atacgaactg
      301 gaatgtttcg atgggccata agctgtgcga tgccgccgcc catgactccc gcaccgagca
      361 atcccatgcg tgtaactttg tctttaggat ccagcttaaa acctggcaca ccgctttgct
      421 ttttaatgcc ttcggtcgca aaaaacagct ctatcaatcg tttcgagacg tccgtaactg
      481 cgagttcgcc gaatgcaatt gattctaatt taagtttttt gtcccgcgat gctcccgcct
      541 gtttattgag tagttctagg attttcagcg gcgccggata cttacctcga gtacgcgaga
      601 gtatcatttt ttttgcttgg ctaaacaaca ctgctcgccc aattgggttc atctcgagca
      661 atgttttttg tagttttgca gtaccagtga ctgcccgttt ttgggagcgg aacttaccgg
      721 ccgcaacgtc cgccgcgatc ttcaaaacac ggtttttaaa atcttgagcg ggaacacagg
      781 cgtcggctag tccaaccttg acagcctttt gcccatccat tgttttaccc gaaaggatgt
      841 agtccatcgc agcttgtaaa ccaatgcgtt ctggcaagcg cacggtaccg ccgaagccgg
      901 gcaaaattcc taaattcact tcagggagtg caatcttagt agagggggaa tcggaggtca
      961 caatcatttt gcaacacaac gcgagttctg tacccccgcc catgcacgct ccgttaacgg
     1021 ccgcaataac cggaaatttt aacttttcaa gttttgcgat caccgtttgc ccacttgcag
     1081 ccagtttagt gcccgtttct ttgtcgtgca ccccttgaat catttgaatg tcggcgcccg
     1141 cgataaaaat tccctcttta tccgaaaaaa tgaccagagc tttgatatcg gatcgagtgg
     1201 cgagcttctg aatttgtgca tcgagctcac ccatgacttt atcgtctagc ttgtttactt
     1261 tttgaccggg taaatcgaaa tgcaaaccaa acacgcccgg aaaagtggtg ttgtagtcca
     1321 ctacgcgaaa agtagagccc gtatccgagc tcgccgactt agaatgctgt gttttttcca
     1381 tgctaatacc gccgttcatc gaacccacct tttgattaaa ctttttctac gattaaactg
     1441 ccaccttggc caccgccgat gcacaaagtc gccaatccaa actgcacgtc tcggatttgc
     1501 atttctttgg ccagcgtgag cacgagccgc gcgcccgtag tgccgacagg gtgccccaag
     1561 gcgatcgcac caccgttcac gttgagtatc tcgtcgcgaa tctcgcccac ggcttcgcta
     1621 cgacccagtt tttcctgagc gaattttttg gatgctgaca ttttttggac cgcaataacc
     1681 tgagcagcaa atgcttcgtt gagttctacc aaacccatat ctttcatttg gaggcctgcc
     1741 gcatcgagtg cttttggcga tgaatacgca ggccccaaac ccattcgttc tggttcaaga
     1801 ccagcccacg caaatgagcg aaccttgccg agcggccgat atcccaaagc cttagctttg
     1861 tcggctcgca tcagaataag catgcacgcc ccatccgtaa tcggacacgc gttacccacc
     1921 gttaccgtgc cgttggcgcg gtcgaaatac ggttttaact tcccgagagc ttcaagagat
     1981 tgtccttttc gcggcccgac gtcttcttct agaaatttgt cgtactttgg cggaagagga
     2041 atcggagaga tttctttttt aaaacgtccg ctctcggtag ctgccacggc cttttcgtga
     2101 cttcgcactg cgaatctatc ttggtcgtca cgtgtgatac cgttttcttt tgccaatagt
     2161 tcggctgttt gtcccatatt gagtccacaa acggcgtccg taagcccctc ttggattgca
     2221 ataattggtt ttagaaatcc tggacgaaac ttagtaagag catttaattt ggcggccggc
     2281 gtttttgcag tcatgagctg gccaaaaaga ttggtcatgg gtttagaata aatgagaggc
     2341 atgttgctca tactctcggt accacccacc acgacgatgt cggccaaacc cgatttgatc
     2401 ttgattactc cttgggtcac cgattccagc gccgatgcac aattgcgatg cacggtatag
     2461 gccggaacat tttgtggaat cccagactct agtgcgatga tgcgcgaaat attcgcggcg
     2521 tcttcgggat tccctgtatt accgatcacc acttcgtcaa ccgttttggg atcaagcgac
     2581 attttataga gcagatcccg cagcgtatgg acgccgagcg tcactgcttt catatctttc
     2641 aacgcggtgc ctgatttggc aaacggcgta cgcaatccat caacgatcca aacttcaggt
     2701 ccctggttga aaaactcagt tgaagacatt gggagctcct ctcgtattgt cggtaagcga
     2761 gaggttagat cagttctttt aaaagtgcaa cgagagtaca aacagatgcg gccgacaagg
     2821 tgccccttgt cagccgcatc tttgcatcga atgaaacgaa gtcgaacttt agaagctaat
     2881 cgatacgcca gccgacaact gataaaagtt gtttttggct aacgcgtcgc cagcagaaga
     2941 tttcgaatct tggttgtagc cgtagccatt gtaaccaaga ccaccgtaat acaatccacc
     3001 gttatagccg ttggaaccga taaacgcgta tgggttcaaa ggattcgtct ggctgctcgt
     3061 gaagatgttg taatatcgaa acatccctac aagcgaaatt gctttggtaa ttcgaaactc
     3121 taagcccgcc tcaagataac ctgaaaaacc ggaaatctcg acgtccgacg ctccataggg
     3181 attcactcgt ttgatatagt ccaaagtacg gtcatcgtaa ttcacatacc ctttgcggta
     3241 agcgagacca gctcccacga aaggacgaac acgtgacttg cgaccgagaa ggttgccctt
     3301 cacaccaagg tcaatcacgt tgtcgttcat gttgagcttc tgcaaataac cagtattaaa
     3361 actattcgga tacatgagcg tgttgccagc accgatggag tacttggcaa acgtataccc
     3421 ggcttgaaag ccgatatggt cggttgcatc aacgccgata gttatgccgg tggcaaactt
     3481 cgactcaacg tcatacatcg aacccgtgat gctcgaaaga ccaactcgag gcatgatgct
     3541 tacgcgacct tccaaggctt catcgtcttc cgacttcgga gcatctactg ctaccattgt
     3601 accgtctacg cttgaagccg aagctcctgg accagctatt ggagtagttc cagttgctgg
     3661 actcgccaac gctgggtctg tcgccgcaga tccaactcgt tgttcaacca acgtcgaaga
     3721 ctcagccgcg ttcgatttct ccacgcccga aaccattatt tggtccgtac gcttcatctc
     3781 gtctttcaaa cgcaattctt ccaatttctg ggtaagcaaa tcttcgttct ttaactcttc
     3841 tcgcaagcgg tgccgacgca tcgcttccga tcgagtgatc tgtctgcgct cttcaattcg
     3901 cggagccgct actggttcgg aaaccacggg ggcagcagga gctacctcat ttgtggaacc
     3961 ttccattgcc aattcgtcct cgaaaaccaa ttcgcccctt acgtctttga tcccgtaaaa
     4021 ggtcacacct aaaaaaacta taaatatggc cgcaaagcct aaacgtttca tttcgaactc
     4081 cttcttttcg tccaagggcc cctagagcaa ttcccatgcc acgaagtcaa ctatccatag
     4141 agacacttcg aagtccgata aaagtggtca acttggccct acctaaagcc cgattccgag
     4201 cgggaaccta atttttttca tttaatatcc tttagttacg acgaattgtt taaaactcag
     4261 acagccctga aagaattccc tgtctaagga tttgacacct gacgagcgcc tgatgactta
     4321 aatcttctca aaggaagttt tcggatttta acgcgccccg ttttttttct aaaataatca
     4381 ttgcgttgga acatgcagcg gcatataacg cgtcctcggc attgcacttg caataccata
     4441 gggcaacaga cggattggat aatctaacta ccccctatac ccccttaagt ccccttttat
     4501 ccaattcgtc tgaacccctt tttctcccta aaaaaaaaat caaaaaaatg aactccgata
     4561 acgaagtaat cactcgatag tttgctttcg tgcgggcgaa gtattcgaaa at
//
LOCUS       DAOMXO010000098         4610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10011_length_4610_cov_5.015148, whole genome shotgun sequence.
ACCESSION   DAOMXO010000098 DAOMXO010000000
VERSION     DAOMXO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10011_length_4610_cov_5.015148"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLH57_03125"
     CDS             <1..457
                     /locus_tag="PLH57_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRK01631.1"
                     /translation="LKKNTGVLQENVTKLKSELEVAQKSVEDIQTQISAIEATRASES
                     EKIGGESKEFDTELGRLSGLRAQAVVGIDVRHLSQYDRVRSGRVGGVGIVPAVSGNCK
                     GCNMRIPPQIYNELQRGNELHFCPSCRRILLYKEAADTQKSSGSSAGSH"
     gene            510..985
                     /gene="rnpB"
                     /locus_tag="PLH57_03130"
     ncRNA           510..985
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLH57_03130"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1124..4168
                     /locus_tag="PLH57_03135"
     CDS             1124..4168
                     /locus_tag="PLH57_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01632.1"
                     /translation="MTGCSPIFPCSRPRPSKCNVAFAAGLHFALVLAFVVSCTIPSFA
                     QDSGKNLSNNSFSHLTFERPVIRSIQEDQLQRLETHRVSATIQFSIPTSHYDKDKDPL
                     SYLMYRLPDYFPAHNIPAHRRVHSTFLHLDDLSLEELREVAWFLSNIPASVVERFGLN
                     RRFKIYDGNEKPKFRRVGVESVFLALEPPREEIEFQVDFYRAVQFCASATSDEVSPRM
                     RAAAKYIVDKHLSHFPEPEKKASAFSDTVPHYSLVQLRRKSLDKGSSKPSQTESLHAA
                     VSEDEFNRAASKVGEIIESANQRYRELNQGRSIERFMSLETNPLEVVVAPRSRGEEPL
                     QAFALKMNSRSEWMPSSVLRFFVPASDVQENSDFVHTEIVEFYTDKLLADGEFSKALS
                     NALVPWRSDINAWSEPRESLTPANLDGFLSEISDERLRSLYLRMHENREISPSQLPAV
                     VEVNGRRVFVTNQVKGTIAFDKGNEQLSMARELITKADHDPKSPFSVSFGYSLYASRG
                     DRVYSWSGDTDIAIGILVHIPENLVLTTEQRDEYVAQALEKEFIEVLLRKAEGGEIAI
                     IELRAGSARKHGSGGNPINDIDDAYFFSEIDLKRGFTIDPATKNKIYLREVLRENDWI
                     KGKFEYVSSGEQFSIQLNVGSDGTTELGYRVVVPGQRDAIKGIPPLTRGGKMLDAESM
                     AVFTALHSGYMVHAVQKGSMAARAGSEVHSAAFAKERFEPIWNAKGAKKTHSLEVFFS
                     QGGTGFDEIGAVRVIEILKFQNALPEELGELIRQRGIYAVLDRLKNTLRSAFNQPTLG
                     LLSSVRRTVNDLREYNERGHEITEKMWRQRQAAVKEVLPKLKSVYQQHVSQDQISQMP
                     EFEKAQLRFENVMDRLGRLPFEQVKKELIKPAYYRVFRRFESQLTELDRRFILAELSG
                     ADRVFASTLIRYFPFIYLEYIQSRKQTSDQDFLLEVQLLGVEPTQENIARLRDVKKVF
                     TLSPASLERLEMELFGVGFREDNSGEINLEKFAADCNDVIDS"
     gene            4185..>4610
                     /locus_tag="PLH57_03140"
     CDS             4185..>4610
                     /locus_tag="PLH57_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01633.1"
                     /translation="MNQRLVAWILVISFISSPFGGLGYAADSKVESNLRSTCNAVLAL
                     TVDIPLTSGREIIAFSKQLGKFAVSPVQTTKDFVDVAITPFGEARHSPLYYVSIVGPI
                     VDAARDSYRRRGALIDAPLGGIPVATAGGFIAAWTIQGLH"
BASE COUNT         1248 a         1012 c         1151 g         1199 t
ORIGIN      
        1 gctcaagaaa aacaccggtg ttttgcaaga aaacgtaact aagcttaaat ccgagttaga
       61 agtggcccaa aaaagtgtgg aagatattca aactcaaatt tctgcaatcg aagcaacccg
      121 ggcctcggag tccgaaaaaa tcggcggaga gagcaaggaa ttcgacactg aattgggacg
      181 attgagcggt ttgcgcgcgc aagctgtcgt aggaatcgat gttcgacatc tttctcaata
      241 tgaccgtgta cgaagcggtc gtgttggtgg agtcggaatc gttccagcgg tttcaggcaa
      301 ctgcaagggc tgcaatatgc gaattcctcc ccaaatttat aatgaattgc aacggggcaa
      361 cgaactacat ttttgtccaa gctgccgtcg tattttgctg tataaagagg cggctgatac
      421 ccagaaaagt tcgggttcct ccgcgggcag tcactagtct tacgatttaa tcacctagca
      481 ttaacaacat cgctctttga aaagttttcc aaacgcgacg gatgaccgct ggtttaacga
      541 ctggaggaaa gtccgggcac tatggaatag agtgctggct aacggccagg cgcagctccg
      601 gtgacggagg ggcgacggaa agtgcaacag aaaataaact accgctgatt cgggttttcc
      661 tgagtcgcgg tgagggtgaa aaggtggggt aagagcccac cggatccctg gtaacagggg
      721 tcgcatggca aaccccactc ggtgcaagat caaatagggg aacggtattt gtttcagagt
      781 ccgtaagcaa cttgagaaag cataagcctc tgaaaacata ggttattccg ccgagcaaat
      841 ggtcgagtaa tcggtctttt gccgcgttcc cgggtaggat cgctagagct tatcggtaac
      901 ggtaggccca gaggaatggt catctcggcg ctctttattt atggagtcgc taggacagaa
      961 cccggcttat agtcgcgttt ggaaattttc aaaaaatcac accctaacac aatccaaaca
     1021 aattaacatt tggtcgaccg cctgcgccgt gttatatggc ccgcgcaatc aataaaaaca
     1081 gctttgttgt ttaggaacct tcctaaagac gaggacgaat aagatgaccg gctgctcacc
     1141 gatctttcct tgttcccgcc cccggccctc aaagtgtaac gttgcttttg ctgcgggact
     1201 acactttgcg ttggtattgg cgtttgttgt ttcgtgcacg attccctctt ttgctcaaga
     1261 ttccggaaaa aacttaagta acaactcttt cagtcatttg acatttgaac gtccagtaat
     1321 tcgttcaatt caagaagacc agctgcagcg ccttgagacc catcgtgtta gtgccactat
     1381 tcagtttagt attccaacgt cgcattacga taaagataag gacccccttt cttacttgat
     1441 gtatcgactg cctgactact ttcctgcgca caacattccg gcccatcgac gcgtacatag
     1501 tacgtttctt catctcgacg atttaagtct agaagaactc cgtgaggtgg catggtttct
     1561 atcgaacata ccggcttcgg tcgtggaacg tttcggattg aatcgtcgat tcaaaatata
     1621 tgacggtaat gagaaaccaa aatttcgccg tgtaggtgtt gaaagcgtgt ttttagcgct
     1681 cgaaccgccc agagaagaaa tagaattcca agttgatttt tatagggcgg ttcaattctg
     1741 cgcttcagct accagtgacg aagttagccc gcgcatgaga gctgctgcta agtacattgt
     1801 cgacaaacac ctttctcatt ttccggaacc ggaaaaaaag gcaagcgcat tttcagatac
     1861 agttccacac tactctttgg ttcaattgag aagaaaatcg cttgataagg gctccagtaa
     1921 gccttcgcag acagagtcac ttcacgcggc cgtctcagaa gatgagttca accgcgctgc
     1981 tagtaaagtt ggcgaaatta tcgaatccgc aaatcaacgc tacagagaac tcaatcaagg
     2041 tcgttcgatc gaacgattta tgagtcttga aacgaacccg ctcgaagtag tagttgcgcc
     2101 gcggtctcgt ggcgaagaac cgcttcaggc atttgctttg aaaatgaatt cgcgatctga
     2161 gtggatgccg agcagtgtac ttcggttttt tgttcctgcg agtgatgtgc aagaaaatag
     2221 cgactttgta cacaccgaga tagttgaatt ttacactgat aaattgctcg ccgatggcga
     2281 attttcgaaa gctttatcga acgcgctcgt tccatggcgg tctgacatta atgcttggtc
     2341 cgagccgcgt gaaagtctca ctccggcaaa cctcgatggc tttttaagtg agattagtga
     2401 cgaacgttta agatctctct atttgcgcat gcacgagaat cgggaaattt caccgtccca
     2461 gttacccgcg gttgtcgagg taaatggacg gcgagtgttc gtaaccaatc aggttaaggg
     2521 caccatcgcc tttgataaag gtaacgaaca gctaagcatg gcgagagagt taattactaa
     2581 agcggatcac gatccgaaga gcccattttc ggtttcgttt ggatactcgc tctatgcttc
     2641 tcgtggagac cgggtttata gttggtcggg cgacactgac attgcgattg gtatattagt
     2701 tcatattcca gaaaatctcg tgctcacgac tgaacaacgt gacgaatatg tagcccaggc
     2761 acttgaaaaa gagttcattg aagtactttt acgaaaagct gaaggtggcg agatcgcgat
     2821 tatcgaattg cgagctggtt ctgcccgtaa acatggttcg ggcggaaacc cgattaacga
     2881 tatcgatgat gcctactttt ttagtgaaat tgatttaaag cgcggcttta caattgatcc
     2941 cgcaaccaaa aacaaaattt atctgcgaga agttctacga gaaaacgact ggatcaaagg
     3001 caagttcgaa tatgttagtt ccggcgagca gttttcgatt cagttaaacg tcggttctga
     3061 tgggactaca gaacttggtt atagagttgt agtaccaggc cagcgcgatg cgattaaggg
     3121 aatccctcct ctgactcgcg gtggaaaaat gttagacgca gagtctatgg ccgttttcac
     3181 ggccttgcat tccgggtaca tggtccatgc ggtgcaaaaa ggaagcatgg cggctagagc
     3241 aggttcggaa gttcatagcg ctgcgtttgc caaggaacga tttgaaccaa tttggaacgc
     3301 aaagggcgca aaaaaaaccc acagtttaga agtctttttt agccaaggtg gcactggttt
     3361 tgacgaaatt ggtgctgttc gtgtcatcga gatactcaag tttcaaaatg cactgcccga
     3421 agaactaggc gagttgatta gacagcgcgg aatatacgct gtgttggacc gccttaaaaa
     3481 cactcttcgt tcggcattta atcagccaac gcttggcctt ctgagttcgg tgcgtcgaac
     3541 ggtaaacgat cttcgagaat acaacgaacg gggccatgaa attactgaaa aaatgtggcg
     3601 ccagcggcaa gcggcggtaa aggaagttct gccgaagcta aagtccgtct atcagcaaca
     3661 cgtttcgcag gatcaaatca gccaaatgcc tgagttcgag aaagcccaat tgcgtttcga
     3721 aaacgtaatg gatcgtttag gaagattgcc atttgagcag gtaaaaaagg aacttattaa
     3781 acccgcttat taccgagttt ttcggcgatt tgaatctcaa ttaacagaat tagataggcg
     3841 tttcatttta gctgaactct cgggagccga tcgagttttc gctagtacac ttattcgata
     3901 ctttccgttt atttatttgg aatacattca gagtcgaaaa caaacgtccg atcaggactt
     3961 tttattagaa gtgcagctac ttggtgtgga accgactcaa gaaaatatcg ctcgtttgcg
     4021 tgatgtaaaa aaagtgttca ctttgagtcc tgcgagtctc gagcggttag aaatggaatt
     4081 gtttggtgtg ggatttcgtg aagataactc tggcgaaatc aatttagaaa aatttgcagc
     4141 cgactgtaac gacgtcattg atagctaaag taaggtggta ttctttgaat caacgtttgg
     4201 tggcctggat cctggtgatc agctttatat cgagtccatt tggtggactt ggatatgccg
     4261 ctgacagcaa agtagagtca aatttgcgat ccacctgcaa cgcagtttta gcactcacag
     4321 tcgacattcc tcttacctct ggtcgcgaaa taattgcttt ctccaaacag cttggaaaat
     4381 ttgcagtgag tccagtccaa acgacgaaag acttcgtcga cgtggcaatt actcctttcg
     4441 gggaggcgcg ccattcgccg ctttattatg tgtcgatcgt agggccaatc gtcgatgcag
     4501 cgagagatag ctatcgcagg agaggagctt tgatcgacgc gcctttggga ggtatacccg
     4561 ttgctacagc cggggggttt atagccgcct ggaccattca agggctacac
//
LOCUS       DAOMXO010000099         4589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10072_length_4589_cov_6.058888, whole genome shotgun sequence.
ACCESSION   DAOMXO010000099 DAOMXO010000000
VERSION     DAOMXO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10072_length_4589_cov_6.058888"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="PLH57_03145"
     CDS             <1..1479
                     /locus_tag="PLH57_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRK01634.1"
                     /translation="ENRTLFFGLWWKELDNENLTEGLKKLNLGATTNGSKFPSMVGER
                     VRLPNESIVGSETSKNVLGPHNDPRLRSIKKETLGRESGTEKFETASADTRSALDWLS
                     DLNAKDSLEVSEPNVMQAVTPANSAPSAVVVSNVGVTSAGTEHESRVPGGRKDPTSSR
                     AAPVNLAKSTRPESQVAPMPSAEGKIPVENGVVPKWESPVQVLSVEGKGDDSAQRAIP
                     ARNPGVSLANSEDYVNSRVERQAKKGSAKVLGERVEAQLKSVDSGREANNAPSVERKE
                     FEMLIPSVREWSHPIESKNSSAGETKALGSLVTAQSTPGTVDLSSPALMAMRVGDLAS
                     RGGGSIRIRVMPEHLGEVTISVSNHQGKLKVALDADNAEAQALLSRHAPELKGILEAS
                     RFQVDRIDVNRETGLTASSSWLASSPVSTSVGSSVSSVSSGQWTGLSNSGNQGGGQGS
                     GFDQSRQDGFMRWDDYNSQWQDQMNGREERARKLWQYAQANA"
     gene            1558..2709
                     /locus_tag="PLH57_03150"
     CDS             1558..2709
                     /locus_tag="PLH57_03150"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HRK01635.1"
                     /translation="MINTLKPASQVGHDDQRGEVLERIAKEYGHPEQKDREPVKKLGK
                     DEFFKIMVTQIQHQDPTKPYQNEQMASQMAQFSALEQMMNVNSNLEKLSESQQPLHHL
                     GAAGLIGKYVTADSSRFVHKEGKYSDLRFDLPADAKKVRLMILNERGETVREIEYGEM
                     KKGTQTVAWNGKRGNNTNAPNGNYMIQVTALNERNQSIPVQTLNTNVVHGVAYEGKDT
                     VLLIGDIRKPQKILLKHVSKIVDRSQAQGASAQHGVAGAPDIDLGAIQQALQNSAQAA
                     ATPGENSGENTEASNDADEGERAVEDYVPGRDAAYAPMNMKMIQAMSQKPQIEQPLSN
                     QEIAAQLEKLNDLTAANPNAAKIQGGARPDLPSNPSLPPSNDGGIVGNW"
     gene            2736..3155
                     /locus_tag="PLH57_03155"
     CDS             2736..3155
                     /locus_tag="PLH57_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HRK01636.1"
                     /translation="MKVSNTFLIPNVSGGPGNIVNRENTQAPDPIGTKGIESNESFSQ
                     ILGNQLEKPIAEPLRFSAHASARIQSRKLNLGADEMRKLGEAVDRAAAKGLDDTLILT
                     KDAAFIVSVKNRTVVTAMDINSMQGNVFTNIEGAVIV"
     gene            3290..4570
                     /locus_tag="PLH57_03160"
     CDS             3290..4570
                     /locus_tag="PLH57_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRK01637.1"
                     /translation="MGILSSLYTGVSGLQANGEALGIYGDNLANANTTGFKTSRPEFQ
                     DVIAKSLKGILGGNQIGRGTKLSNVNPVFAQGSLTQTDRATDLAISGDGFFVLDGPEG
                     RCFTRNGVFNFDKDGRLTSLEGARVMGFQADENGLITNKLTEIKLDRTVIDAKRTSKV
                     NLSMNLDIRAEKGLDFNLENPDKTSKYSNGFTVYDSAGNAHLVTVYFNKVEDGQWKWH
                     AVVKGEEVQGGEKGKLVEAASGTLKFGTDGRLQEAATDKNEFNFNKGALPGQKIEFNF
                     GLTVMEGGDGLQTTQYGTNSDLYKHTQDGYTAGTVAGLSFNDDGVLSAIFTNGETLNL
                     AQVAIGKFENNEALFKKGQNQYRESKNSGQAFIGKPGDGGRGRITAKSIEGSTTDIAA
                     EFINLMTAQRSFQANARAITTADELMAEVLNMRK"
BASE COUNT         1332 a         1001 c         1257 g          999 t
ORIGIN      
        1 gagaaccgta ccctgttctt cggtttgtgg tggaaggagc tcgacaacga gaacttgacc
       61 gagggtttaa agaagctgaa tttgggagca acgaccaacg gctcgaagtt cccatcgatg
      121 gtgggagagc gggtgcgatt accgaacgaa tcaattgtgg gtagcgagac gtcgaaaaac
      181 gtactcggtc ctcacaacga tccacgtttg aggtccatca aaaaagagac gttaggtcgt
      241 gaaagtggga ccgagaaatt cgagactgct tcggcggata cacgttcggc ccttgattgg
      301 ctgtcggatc tgaatgcaaa agattcgcta gaagttagcg aaccgaatgt aatgcaggca
      361 gtgacaccag cgaattcagc accgagtgcc gtcgtggttt cgaacgttgg tgtaacgagt
      421 gctggaaccg aacatgagtc gagagtaccg ggcgggcgaa aggatcctac gagttccagg
      481 gccgcgcccg tgaatctcgc caagtcgacg agaccagaaa gtcaagttgc accaatgccg
      541 agtgccgaag gtaaaattcc ggtggaaaac ggagtagtgc ccaagtggga gtcacctgtg
      601 caagttcttt cagtagaagg caaaggtgat gattccgcgc agagagctat tccagcaaga
      661 aaccccgggg tttctttagc caacagtgag gactacgtta atagccgcgt cgaaagacag
      721 gcaaaaaaag gtagtgctaa ggtgttgggc gaacgagtcg aggctcagtt gaaatcggtt
      781 gattcggggc gtgaagcgaa caacgcacca agtgtagaac gtaaagagtt cgagatgttg
      841 attccgtcgg tacgggagtg gtcgcatccg atagaatcta agaactcaag tgcaggtgaa
      901 acgaaggctc tcgggtcttt ggtaaccgca cagtctacgc cggggacggt cgatctaagt
      961 agtcctgcgt tgatggcgat gcgagtaggg gatttggcga gtcgcggagg cggaagtatt
     1021 cgcattcgcg taatgccgga acaccttggt gaagttacga tttcagtaag taaccatcag
     1081 ggtaaactga aggtcgctct cgatgcggac aacgccgagg ctcaagcatt gctttcgagg
     1141 catgcgcctg aattgaaggg cattctcgag gcttcgcgtt ttcaagtaga tcgtatcgat
     1201 gtgaatcgtg agacaggact gaccgccagc agttcatggt tggcgtctag cccagtgagc
     1261 acgagcgtcg gaagttcggt gagtagcgta agctccggtc agtggacggg tctgtcgaat
     1321 tccggtaatc agggcggtgg ccaaggttcc ggatttgatc agtctcgcca agatggattc
     1381 atgcgttggg atgattacaa ttcccaatgg caggaccaga tgaacggtag agaagagcgt
     1441 gcgcgtaagc tgtggcagta tgcacaagcg aacgcgtaaa tagcgtttaa aatggaactg
     1501 agaatccgtt actggaactg gcccagaacg gattctccaa ttccgatgga gtgagtggtg
     1561 atcaatacat taaaaccggc gtctcaagtt ggccatgacg atcaacgtgg tgaagtgctt
     1621 gaacgtatag ccaaggaata cggccatccg gaacaaaaag atcgtgaacc tgttaaaaaa
     1681 ctcggaaaag acgagttttt taaaatcatg gttacccaga ttcaacacca agatcctacc
     1741 aaaccttatc agaacgaaca gatggcttcg cagatggcgc agttttcagc gcttgagcaa
     1801 atgatgaacg taaactcgaa ccttgaaaaa ctttcggagt cgcaacaacc gcttcaccac
     1861 cttggagccg ctggattgat tggaaaatac gttacagccg attcgagtcg gtttgttcac
     1921 aaagaaggca agtattccga tctgcgtttt gatttgccag cagatgccaa aaaagttcga
     1981 ctcatgattc taaacgagcg cggcgagact gtacgcgaga ttgaatacgg cgaaatgaaa
     2041 aaaggcactc aaaccgtagc ctggaacggt aaacgcggga acaataccaa cgctcccaac
     2101 ggcaactaca tgattcaagt taccgcgctc aatgagcgca atcaaagtat tccagttcag
     2161 actctaaaca ccaacgtcgt gcacggcgtt gcttacgaag gtaaagatac agttttgctc
     2221 attggcgata ttcgaaaacc acaaaaaatt ctgctgaaac acgtgtcgaa gatcgtggat
     2281 cgtagtcagg ctcaaggcgc gtctgcccag cacggggtcg ccggtgcgcc ggacattgac
     2341 cttggagcga ttcagcaagc gcttcaaaat agtgcccaag cggccgcaac tcccggtgaa
     2401 aacagtgggg agaataccga agcgtcgaac gacgccgacg aaggtgaacg agcggtcgaa
     2461 gactacgtac cgggccgtga tgcagcctat gcgccaatga atatgaaaat gattcaggct
     2521 atgagtcaaa agccccaaat cgaacagccg ctcagtaatc aggaaatagc tgcgcaactt
     2581 gaaaaattga atgatctaac ggcagctaat cctaacgcgg cgaagattca aggtggcgct
     2641 cggccggatt taccgagcaa cccaagtttg ccccctagta acgacggggg aattgtagga
     2701 aactggtaag gtagtaccaa aaaaagaaag gaggtatgaa agtgtcgaac acgtttttaa
     2761 ttcccaatgt ttctggcggc cctggcaata tagttaatcg tgaaaacacc caggcgcccg
     2821 acccaattgg cactaaagga atcgaaagta acgaaagttt cagtcagatt ctgggaaatc
     2881 agctcgaaaa accaattgcc gaacccttac gtttttcggc tcacgcttca gcaagaattc
     2941 aaagtcgtaa gttgaatctc ggagccgatg aaatgcgtaa gcttggtgaa gcagtagatc
     3001 gggcagcagc gaagggtctt gacgatacgc ttattcttac aaaagatgcg gcgtttattg
     3061 tgagtgtcaa gaatcgtacg gtcgtgacag cgatggatat taattcaatg cagggaaatg
     3121 tgttcacgaa tattgaagga gcagtgatcg tttagagttc taggatggaa cggtaggcgg
     3181 gtccgcggaa gaggtttatg aaactttcga ccaagggcgc ctccaaaaat aaaaagtgat
     3241 cgaatatcac tttgattgat cgaatcccaa gatagcaagg aggctaaaaa tgggtatatt
     3301 atcgtctctc tataccggcg ttagcggttt gcaagcaaac ggtgaagcgc tcggaatcta
     3361 cggcgacaac ctcgccaatg ccaatacgac tggatttaaa acctcaagac cagagttcca
     3421 agacgtgatt gcaaaatcac taaagggtat tttgggaggt aaccaaattg gtcgtggtac
     3481 taagctctcg aacgttaacc cggtgttcgc acaagggtct ttaacacaaa cggaccgcgc
     3541 tacagatctt gcaatctcgg gcgacggttt tttcgtgttg gatggtcccg aaggccgatg
     3601 ttttactcgt aacggtgtat tcaactttga caaagacgga cgtctcacaa gtttagaagg
     3661 tgcgcgagtg atgggattcc aggctgacga aaacggcctt atcaccaaca agctcactga
     3721 gataaaacta gatcgtaccg tgatcgacgc caaacgtacg tccaaagtaa atctttcgat
     3781 gaacttggat attcgggcag aaaaaggact agacttcaat ctcgagaatc cagacaaaac
     3841 tagcaagtac tcaaacggtt tcaccgttta tgactcggct ggtaatgcgc atctcgtgac
     3901 cgtttacttc aacaaagtcg aagacggtca atggaaatgg catgctgtcg ttaagggcga
     3961 agaagtccaa ggcggagaaa aaggaaagct ggtcgaagcc gcgagcggta cattgaagtt
     4021 tggcactgat ggtcgacttc aagaagctgc gacagacaaa aacgagttca acttcaataa
     4081 aggtgcattg cctgggcaga agattgaatt caatttcggt cttactgtga tggaaggtgg
     4141 cgatggtctg caaaccactc aatacggtac gaactccgac ttgtataagc acactcaaga
     4201 cgggtacacc gcaggaacgg tcgcaggtct ttcgttcaac gacgatggtg tattgtcggc
     4261 gatttttacc aacggcgaaa cgctcaatct ggcgcaagtt gcgattggta agttcgagaa
     4321 caatgaggcc cttttcaaga aaggtcagaa ccagtatcgg gagtcgaaaa actctggcca
     4381 agcgtttatc ggaaaacctg gagatggtgg gcgaggtaga attactgcca agtctattga
     4441 gggttcgact acggacattg ctgcggagtt tattaacttg atgaccgctc aacgatcgtt
     4501 ccaagcgaac gctcgtgcga tcacaaccgc cgatgaactc atggccgaag tcttgaatat
     4561 gcgtaaataa tagctaaaat ttacggttg
//
LOCUS       DAOMXO010000100         4581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10090_length_4581_cov_5.442333, whole genome shotgun sequence.
ACCESSION   DAOMXO010000100 DAOMXO010000000
VERSION     DAOMXO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10090_length_4581_cov_5.442333"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..771
                     /locus_tag="PLH57_03165"
     CDS             205..771
                     /locus_tag="PLH57_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01638.1"
                     /translation="MQTSPGTGNILRVLMRLALRKAVFILGLSLMLNLIMDLVNSPRA
                     VAAYTLSDAGETVGIATGIGAVIGFSTIAFYEQPFSHMTNVLVGAGAGMLVGLGVAAY
                     MTASDDEADISPEELLPPVKKPSDLKQLEKNPKNPIQNGYYKSRKRMYTPEAPRSIRL
                     GAQAHDLGSMAYAQRGWSVAARVVELRF"
     gene            772..4476
                     /locus_tag="PLH57_03170"
     CDS             772..4476
                     /locus_tag="PLH57_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRK01639.1"
                     /translation="MPKNELIINATLPETRIALLEDGEIQELHVERDSERGVVGNIYK
                     GKVIRVLPGMQAAFVDIGLEKAAFLYVDDVHYGEMNEPLVMPEFREMPIETRRPAPVQ
                     LPLPTNEVSAVETSYPQYREVVRESIEQAGGTLESPEGAPNAGETSDPMESEEDRQPD
                     NLEPRAYIPPRISHGAGPHGPRRGGRDDSRRGGRGRRGGRGRRGGVGRNQHANLGGRG
                     PGRGRPVFGGPARAIGPVNPGNPGSPEDHAALQREYARRMLDVEDDNFDQPVEKSEIS
                     ASELRNESSKDLSNDQPVEQVEVQAQLSPKIEAESEGDSIDVAAWTNERVFHPLRGEV
                     VGPIEESGEENSADEGLNDSTDEETEDSEESDESYEAANESTESGGDQDRASLTSDES
                     GAEGMAESSEEIDEVSGEEDLENQDDAGEEAASLALQPGASEAQPIAARGAPESAGDL
                     RSRGRGGRSRGRRDRHRGRRRTGGGPGGYSTEPRQPHSSGGNSDDDIEEMPKRRKRRD
                     EVNIADLVREGQEILVQVAKEPIGTKGARLTCHVSLPGRYLVFMPTVDHIGVSRRIEG
                     DGERRRLKDTISAFKPAGTGVIVRTASGKQTDRKLWADLDFLVETWNEIQTKYQKSKG
                     PTLVYQDLTFVLRAVRDMFTDDVDKIVVDSPREHKAIMKFIMRFMPNVEEKIIHYNGK
                     LPIFDEYGIETELERALERKVWLKSGGYLVIDQAEALVAIDVNTGRYVGKKNLEETIL
                     KTNLEAVREIAYQLRLRNFGGIIILDLIDMEKESNRDRVYKALEEELKKDRARPTIMK
                     ISQLGLIEMTRKRTRESLVRSLCESCNYCNGRGYVKGHKTVSFDILREIEREMQTSET
                     EAMTIQCHPTVADELLGDKRDVIEDLEHQHNKRISIRSNGAYHIEQFELVSHRAEGKD
                     FAQSSDERKKALRARIQARQAELAAVAREERHQQRSQQRDAMDENATGAREGEPSMGG
                     EPRGDRYRGGGEGPRRGGRGRRGRGGRGRGGGRTFEAGAPGPSAAENLDRDAQLTEGY
                     SDNPVASHEGSASDVSGGDTSNESNTGFPGNRGRGRNREADDRYGEDEIFEVVIPASD
                     GGLVGEGDLEAGGIDEGAATPAPVIMPPDRGPGPRRDAQGAGGDGQDRPNRRRGRRGG
                     RFRKRPGGGGGGGRFSGPREGTPQGSGGGRGGPHGGSQRGGRGGPSDGSTRHSPQRPA
                     QIYAADSYSRNTAPVKVAPRSSDDDDFGNR"
BASE COUNT         1197 a         1055 c         1359 g          970 t
ORIGIN      
        1 aaaagcgttc agttcgtctt gaaaaagttt ggtgagtttc atttttttgt cttttatttc
       61 aaatggttga ctactaggcc ggagattacc tcttgtagtg tgacgtaaat tgtaataaca
      121 aagttaacag gagttccaaa gtcgaaccct aagttttttt gacagtgaaa tactaacgca
      181 ttgtgcagga ggatctgtac agacgtgcag accagccctg gcaccggaaa tatattacga
      241 gttttgatgc gactcgcgtt gcgtaaagca gtgttcatcc ttggattaag cctgatgctg
      301 aacctgatca tggatttggt gaatagcccg cgtgcagtgg ccgcttatac attaagcgac
      361 gctggcgaga ccgtaggaat tgctacgggc ataggtgcag tcattggctt ttcgacgata
      421 gcgttctatg aacaaccgtt ttcccacatg acgaatgtct tagtgggagc gggtgccgga
      481 atgctcgtcg gtcttggcgt ggcagcctac atgacggctt cagatgatga agccgatatc
      541 agcccagaag agctcttgcc acccgttaag aaaccaagtg atctcaaaca gcttgagaag
      601 aatcctaaga accccattca gaatgggtac tacaaatctc gaaagagaat gtataccccc
      661 gaagctcccc ggtctattag actaggagcc caggcacatg atctcggttc gatggcttac
      721 gcgcaacgtg gttggagtgt tgcagcaaga gtcgttgaac tgaggtttta gatgcccaaa
      781 aatgaattaa tcataaacgc cacattaccc gaaacccgaa tcgctcttct agaagacgga
      841 gagattcagg aactgcacgt agagcgagac tctgaaagag gagtcgtcgg aaatatctac
      901 aagggcaaag taattcgagt gttacctgga atgcaggccg cgttcgtaga cattggtctt
      961 gaaaaggcag cgttcctgta tgtggacgac gttcactacg gggaaatgaa tgaaccgcta
     1021 gtaatgccgg agtttcgtga aatgccgatt gaaactcgtc ggcccgctcc ggtgcaattg
     1081 ccgctgccta cgaatgaagt ttcggcagta gaaacgtctt atcctcaata ccgcgaagta
     1141 gttcgagaaa gtattgaaca agcgggtggc acgctcgaat cgcccgaagg cgcgcctaat
     1201 gcaggcgaaa cttccgatcc gatggagagc gaagaagatc gccagcccga caatctagaa
     1261 ccgcgggctt atattcctcc gcgaatttcc catggcgccg gtcctcacgg acctcgacgc
     1321 ggcggaagag acgacagccg tcgaggcgga cgaggccgtc gcggtggacg ggggcgtcgt
     1381 ggtggcgtgg gtcgcaatca gcatgcaaat ttgggaggcc gagggcccgg cagaggtcgc
     1441 ccagtgtttg gtggcccagc gcgtgcaatt gggcctgtga acccaggcaa cccagggtcg
     1501 ccggaagatc acgcagctct gcagcgcgaa tacgcgcggc gaatgcttga cgtagaagat
     1561 gacaattttg accaaccggt tgagaaaagc gagatatcgg cttctgaact tagaaacgaa
     1621 agttcgaaag atctatctaa cgaccaacca gtagaacaag tcgaagttca ggctcagtta
     1681 tcaccaaaga ttgaggcaga gtcagaaggc gacagtattg acgtagcagc ttggaccaat
     1741 gagcgggttt ttcatccgtt gcgtggggaa gtggttggcc cgatcgagga atccggagaa
     1801 gaaaattcag ccgatgaagg gctaaacgat tccactgacg aagaaactga ggactcagaa
     1861 gaatcagacg agtcttacga ggcggccaac gagtcaactg aaagcggcgg cgatcaggat
     1921 cgcgcttcct tgaccagtga cgagtctggc gcagaaggca tggcggaatc ttccgaagaa
     1981 atcgatgaag tttccggcga agaagatctt gaaaatcaag acgacgctgg ggaagaggca
     2041 gcaagtttgg ctctgcagcc cggagcttcc gaagcgcagc caatcgctgc gcggggtgca
     2101 cccgagtctg ctggtgatct acgaagtcgc ggacgtggcg ggcgctcccg tggtcgcaga
     2161 gatcggcacc gtggtcgtcg acgcaccggc ggaggccctg gcgggtattc tacggaaccg
     2221 cgccagcctc acagtagcgg cggaaatagc gacgacgata ttgaagaaat gccgaagcgt
     2281 cgtaagcgtc gcgacgaagt aaatatcgca gaccttgtac gcgagggtca ggaaatttta
     2341 gttcaagtag cgaaagaacc aattggtact aaaggggcgc gtttaacgtg ccacgtgagt
     2401 ttacctggcc gctacctcgt ttttatgccg acggttgacc acataggtgt gtcgcgcagg
     2461 attgaagggg acggtgaacg ccgtcgattg aaagacacca tttcggcttt caaaccagca
     2521 ggcactgggg tgattgtacg aacggccagc ggcaaacaga ctgaccgcaa gctttgggcc
     2581 gatttagatt tcttagtcga gacctggaat gaaattcaaa cgaagtatca aaagtcgaaa
     2641 gggccgacgc tcgtttatca ggatttgacg tttgttctgc gcgcggtacg cgacatgttt
     2701 accgacgacg tagataaaat tgtcgtcgat agcccccgtg agcataaagc gatcatgaaa
     2761 tttatcatgc gatttatgcc aaacgtggaa gaaaagatca ttcattacaa cggaaaactt
     2821 ccgatattcg acgaatatgg cattgaaacg gagctagagc gggcacttga acggaaggtg
     2881 tggctcaagt ctgggggtta cttagttatc gatcaagcgg aagctttggt tgctatcgac
     2941 gtgaataccg gccgctacgt aggaaaaaag aatttagaag aaacgatttt aaaaaccaac
     3001 ctcgaggccg tgcgagagat cgcttatcaa ctgcgattgc gaaacttcgg cggaatcatc
     3061 attctcgatt tgatcgatat ggaaaaagag tcgaatcggg atcgagtcta caaagctctt
     3121 gaagaggaac ttaaaaaaga tcgggcgcgt ccgacaatca tgaagatcag ccaattgggt
     3181 ctgatcgaaa tgacccgcaa gcgtacccgc gaaagtttag tgcgctcgct gtgtgagtct
     3241 tgcaactatt gtaatgggcg cggttatgta aaaggtcata aaacagtttc atttgatatt
     3301 ttgcgtgaaa ttgagcgtga gatgcaaact tcagagactg aagctatgac aattcagtgt
     3361 catccaacgg ttgctgatga gttgctgggt gataaacgcg atgtgattga ggatcttgaa
     3421 catcaacata acaagcggat ttcgattcgt tcgaatggcg cctatcatat tgaacagttt
     3481 gaacttgtct cgcaccgtgc tgaggggaaa gattttgcgc agtcctcgga cgaacgaaaa
     3541 aaagcactcc gtgcgcgtat tcaagcacgc caggcggaac tggcggctgt tgcgcgagag
     3601 gaacggcatc aacaacgatc gcagcaacgt gatgctatgg acgaaaatgc gacaggcgcc
     3661 cgcgaaggag agccgtcgat gggcggcgaa ccgcgaggtg atcggtaccg aggcggaggc
     3721 gaaggtccgc gtcgcggtgg acgtggacgg cgcggccgtg gtggcagagg ccgtggcggc
     3781 ggacgcacgt ttgaggcggg cgcacctgga ccgagtgctg cagagaatct agatagagat
     3841 gcgcagctga ccgaaggata ttcggataac ccagtcgcta gtcacgaagg ctcagcgtcc
     3901 gacgttagtg gtggagatac ttctaacgaa tcgaatacag gttttcctgg caatcgcgga
     3961 cgaggtagaa accgtgaggc tgatgaccgt tatggcgaag atgaaatttt tgaggtcgtc
     4021 atacctgctt ccgacggcgg tttagttggt gaaggagatc tcgaagctgg tgggatcgac
     4081 gaaggtgctg ctaccccagc gcccgtgatc atgccgccag accgtggacc tggtccgcga
     4141 agagacgctc agggtgcagg aggcgatgga caggatcgtc ccaaccgacg acgcggtcgg
     4201 cgcggtggtc gttttcgaaa acggccaggc ggcggcggtg gtggtgggcg gttctccggt
     4261 cctcgtgaag gtacgccgca gggaagtggc ggcgggcgcg gagggcccca tggcggatcc
     4321 cagcgcggag gtcgcggagg gccatcagat ggttcaactc gccattcgcc acagcgtcca
     4381 gcacaaattt atgcggcgga ttcctattcc cgcaacacgg cacccgttaa ggttgcgccg
     4441 cgctcgtctg acgacgacga ttttgggaat cgataagcga tccaacggaa agtttgggtg
     4501 tgaaaaaaat acacactcaa acttgttgga atcataaaaa atttgcttac cccactggac
     4561 tcgccgtgaa ttttctgcga t
//
LOCUS       DAOMXO010000101         4575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10107_length_4575_cov_4.258407, whole genome shotgun sequence.
ACCESSION   DAOMXO010000101 DAOMXO010000000
VERSION     DAOMXO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10107_length_4575_cov_4.258407"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..549
                     /locus_tag="PLH57_03175"
     CDS             112..549
                     /locus_tag="PLH57_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01640.1"
                     /translation="MKSRLRILIYPRFQLLLLGINFLVFAIVSGVMFWQHQRTQGAIR
                     QLGLDANLSPSHGFFQLVELQSQQYLASLTAVMLFGFIFSALVMLVVSNRLAGPIVRL
                     RRYFGDIAIKTDNVPPLSFRKGDFFGDLPEVVNRAVQALQTKK"
     gene            complement(556..3609)
                     /locus_tag="PLH57_03180"
     CDS             complement(556..3609)
                     /locus_tag="PLH57_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRK01641.1"
                     /translation="MASGCLSSGGGGASGGGSGGGSGGGGGSSSTTGSAPIITPAPIP
                     IIPSPRIVSPVLNPLQSNSDHVVITGECDARYALLVSDGGAEAQELTCTGSGEFSYPA
                     TKSDDGTYLFSFFHDDRSGVSSQAVIFNWTLDRRAPAPPRVTSPASPTHVSYQNELTI
                     LGSCEPGATVFLEHTDGRRESVLCQNESFTFRVLGESDGSYTFAFSQTDWAGNPSASS
                     QLVWRRDSSVPVSPTIVRPNVNPLNTRLRNVEFELSCQPNASGEVETEFGKIPFLCQT
                     NGVGYATLDIPPSVTSERVVLARARQFGPGGAPSADVETTIAFDPIAPNAVAFSPSEL
                     ASVEQTHDLLLGCEPLARIDVAIDDPNLVVTNLACGADGQATYEAVGQMSQFRTFYFT
                     QVDRAGNPSVQREFRFYTDPSVPSPVTIAAPNEQPFATRAVDFTVRVFCSPGNTVQVE
                     GLSVETPIQKICDSSGSAYFELHESAEGAHSVYFKQMQAYTLVESSPTRFDWVLDTTP
                     PTVAIVSPMAQPFYSPTAITVEGTCGDARVVRGFEPSVGEMLITCSSGTFEVYLHQSN
                     DGTYSYTFSATDDVGNPSNEVTFRWVRDTAGPSAPMIQWPPQAWFLTQSDTMNVRGTC
                     QIGTEMRTLGLVSGGFAAAELAIPCNDGIFEIPLHFAADGNYEIGVYAHKPENGYNSS
                     SVPATWIRDTTPPTFFLGAGPTGSTPSREATAYFWGSGSGDVHHFECRLDGGDYATCT
                     SPHVVQGLGVGAHTLEIRALDAVGNPSLPQGFSWNITDTFETIALYHFDNATGSFADS
                     SYYAQIGSGITIQSFGTGNATGRFAEGRSLTPGFRHYLSIPNEARYDSLRERMIFEMY
                     VSVRKLPAKGRQMALASKGGSTAGSYGWQIYLRRRTGDNKIVLVFRASVDGGNTYDEI
                     VTAPVTISTNTFYHIVYRLDTDHSNAAQVRGFLNGSRVANGSFINDSGHRATINNPDL
                     PLYLGRDASSSAYFDGVIDEVCIHNTTGKYPGTGFPVQVSPCQPQ"
     gene            complement(3817..>4575)
                     /locus_tag="PLH57_03185"
     CDS             complement(3817..>4575)
                     /locus_tag="PLH57_03185"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRK01642.1"
                     /translation="KTEEVVRCEMTSIQKKQYRAFLNSSEAAKLRADIEAGEKIDYAG
                     VLSMLTRLKQVCDHPRLPAYTTGKIKKATKIDPEETGKWEVFVELLEEALRSNLKVVV
                     FTQYLGMIDVIGIWLKKQGIQFTELRGDTRDRGERIMRFQDDPECKVFLCSLLAGGLG
                     IDLTAGSVCIHFDRWWNPAKENQATDRLHRIGQTRGVQVFKLQCPETIEDRIAEIIQS
                     KKTLSGVLIEESPVGLKAFSRDELLALLTDTLHG"
BASE COUNT         1107 a         1235 c         1136 g         1097 t
ORIGIN      
        1 gtttgcgctc tttgtgcacc tcgcgcctat atgaaaaaag gccactgctt cggaaaaaaa
       61 ggcgaatgtc actacgagcg aagcggaaaa aattcggact aaccgcagtt tatgaaaagt
      121 cgcttaagaa tcctgattta tccccgtttt cagttgttac tactgggaat taactttcta
      181 gtattcgcaa tcgttagcgg cgtcatgttt tggcaacacc aacgcactca gggcgctatt
      241 cggcagcttg gactagacgc gaatctttct ccctcgcacg ggttctttca attagtcgaa
      301 ctgcagtcac agcaatatct agcaagtctt acggccgtta tgctttttgg cttcattttt
      361 tcggctttgg tgatgctcgt ggtctcaaat cgtctcgcgg gaccaatcgt gcgtttgcgt
      421 cgctacttcg gcgacattgc gatcaaaacc gacaacgtac cacctttgtc ttttcgaaaa
      481 ggcgacttct ttggcgacct acccgaagtc gttaatcggg ccgtgcaggc gctgcagacc
      541 aaaaaataaa gccacttatt gcggctggca tggactaact tgcaccggaa acccagtgcc
      601 tggatatttt cctgtagtgt tatgtatgca cacttcgtct atcactccat cgaaataggc
      661 actgctcgaa gcgtcccgcc ctaaatacag cggcagatcc gggttattaa tcgtggctct
      721 atgaccactg tcgttaataa agctaccgtt tgcgactcta gaaccattta aaaaaccccg
      781 cacttgcgcg gcgttcgagt ggtcggtatc aagtcggtag acgatatgat aaaaagtgtt
      841 tgtgctaatt gttactggtg cggtcactat ttcatcgtaa gtatttccac cgtcgacact
      901 cgcccggaaa accaggacga tcttgttgtc gcccgtgcgc cgacgcaggt agatctgcca
      961 accataactg cccgccgtgc tgccgccctt tgaagcaaga gccatttgtc tgccctttgc
     1021 tggcaattta cgtaccgaca catacatctc gaatatcatt ctctctcgca acgaatcgta
     1081 acgcgcttcg ttcggtatcg aaaggtagtg ccgaaatccc ggcgtaagcg agcgtccttc
     1141 agcgaacctt cccgttgcgt tgccagtgcc gaagctctga attgtaatcc cagagccgat
     1201 ctgagcgtaa taactgctgt cggcaaacga gccggtggcg ttgtcaaagt gataaagtgc
     1261 gattgtttcg aatgtgtcgg ttatgttcca actaaaccct tggggcagac tcggattacc
     1321 gaccgcatct aaagctctga tctctaaagt atgtgccccg acgcccaacc cttgaactac
     1381 atggggcgag gtgcaggtgg cgtagtcccc tccatcgagt cgacactcga agtggtgtac
     1441 gtcgccgctt cccgatcccc agaaataggc cgttgcttcg cgcgaaggag ttgatccagt
     1501 cggtcccgcg ccgagaaaaa aggtaggtgg tgtcgtgtca cgaatccagg tggcgggcac
     1561 ggaactagaa ttataaccat tttcgggctt gtgagcgtaa acaccaattt cgtagtttcc
     1621 gtcggccgca aaatgcagag gaatttcaaa tattccatcg ttgcacggaa tggccaattc
     1681 cgcagctgcg aaaccaccgc tcactaatcc cagtgtacgc atttcggtcc ctatttggca
     1741 ggttcctctt acgttcatgg tgtcggactg cgtgagaaac caagcctgag gcggccactg
     1801 aatcattggc gcactcgggc cggcagtgtc tcgaacccag cgaaacgtaa cctcgttgga
     1861 cgggttgccc acgtcgtcgg tagccgaaaa agtataactg taggtaccgt cgttggactg
     1921 atgcaaatag acttcgaagg tgcccgaaga acaagtaatg agcatctccc ccacgcttgg
     1981 ctcaaagccc cgtacgactc tggcatcgcc gcaggtacct tccaccgtga tggcggttgg
     2041 ggaataaaag ggttgagcca tgggcgaaac gattgcgaca gtcggcggag tcgtatcgag
     2101 tacccagtca aaacgcgtag gactcgactc tacaagagtg taggcctgca tttgcttgaa
     2161 atacaccgag tgagcccctt cagcgctttc atgaagttca aaataagcgc tcccgcttga
     2221 atcacatatt ttttgaattg gagtctcaac agagagccct tcgacttgga ccgtgttgcc
     2281 gggcgaacaa aaaactctga ccgtaaaatc taccgctcgc gtcgcaaacg gctgttcatt
     2341 cggggctgca atcgttacag gactaggaac gctcggatcg gtgtaaaacc ggaattctcg
     2401 ctgcaccgaa gggtttccag cgcggtccac ctgggtaaaa tagaacgttc ggaattgact
     2461 catttggccc acagcttcgt aagtagcttg gccatcggct ccgcaggcca agttcgtcac
     2521 aacgaggttc gggtcatcga tcgcaacatc gattcgcgcc agcggttcac agcccagcag
     2581 cagatcgtgc gtctgttcaa ccgatgccaa ctcactcgga ctaaaggcca ctgcattcgg
     2641 cgcaattggg tcgaaagcta ttgtggtctc aacatcggcc gacggagctc cgcctggtcc
     2701 gaattggcga gcccgcgcta gtactacgcg ttcgctggtc accgaaggcg gtatgtccaa
     2761 cgtggcgtat cctacgccgt ttgtttggca taaaaacgga atttttccga actcggtttc
     2821 tacttctccc gaagcatttg gctgacagct cagttcgaac tctacgttgc gtaaacgagt
     2881 gttcaacgga tttacattcg ggcgtacaat cgtcggtgat accggcaccg acgaatcgcg
     2941 ccgccacact agttgcgaag aagcgctggg gtttccggcc caatcggtct gggaaaaagc
     3001 aaaagtgtac gacccatcgc tttcacctag cactcgaaac gtgaacgatt cgttttgaca
     3061 caataccgat tctcgacgcc cgtcggtgtg ttctagaaaa acagttgccc cgggttcgca
     3121 cgaaccgaga attgttaatt cattttgata cgaaacatgg gttggcgatg cggggcttgt
     3181 cactctaggc ggcgccgggg ccctgcgatc caaggtccag ttaaaaataa cggcttgaga
     3241 gcttactccc gaacgatcgt cgtgaaaaaa cgaaaataag taagtaccat cgtcactttt
     3301 ggtagctgga tagctaaact caccggaacc cgtacaggtt agctcttgag cctctgcgcc
     3361 cccgtcgctc acaagcaaag catatcgcgc gtcgcactcg cccgtaatca ctacatgatc
     3421 gctattcgac tgaagagggt tcaacacggg cgacactatt cgcggagaag gaatgatcgg
     3481 aatgggagcc ggcgtaatta tcggtgccga accagtggta gaactactac cgccgccacc
     3541 accagagccg ccgcccgaac caccgcccga agccccaccg ccaccagatg ataaacaacc
     3601 ggaagccatc aaagctccga acaatactat ggccgctcga cagctaaaaa acagtcgcac
     3661 gttcctcaat ggaaggtccc cctagaccaa tccattttgc tataccgtat ctatttaatc
     3721 aacaattaat atactgaaat ttatctaata attacagggc attacacgag cttaatctgg
     3781 taaaacagcc gcccagggtg cgactccccc gggtgtttag ccgtgcaggg tgtctgtgag
     3841 gagcgccagc aattcgtcgc gtgaaaacgc ttttaggccc acgggtgact cttcgatcag
     3901 cacgccgctg agagtctttt tactctgaat gatttcggcg atgcggtctt cgattgtttc
     3961 agggcactgg agtttaaaaa cctgcacgcc gcgggtttgg ccaatgcggt gcaaacggtc
     4021 agttgcttgg ttctctttcg cggggttcca ccaacgatca aagtgaatgc acacactgcc
     4081 ggccgtgaga tcgataccca agcccccagc gagcaagcta cagagaaaca ccttacattc
     4141 agggtcgtct tgaaaccgca tgatgcgttc accgcgatca cgggtgtcac cacgaagttc
     4201 ggtaaactgg attccctgtt tttttagcca aataccaatg acatcgatca tgccgagata
     4261 ctgggtaaat acgacgactt tgagattcga gcgcagcgct tcttcaagaa gctccacgaa
     4321 cacttcccac ttgccagttt cctcagggtc aatcttggtg gccttcttaa tttttccagt
     4381 agtgtaagcc ggcagtcgcg ggtgatcaca aacttgtttt agccgggtga gcattgaaag
     4441 aacgcccgcg tagtcaattt tttcaccagc ctcgatatcg gcccttagtt ttgcagcttc
     4501 gctactattg agaaacgcac ggtattgctt cttctgaatc gaagtcatct cgcaacgaac
     4561 cacttcttca gtttt
//
LOCUS       DAOMXO010000102         4543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10206_length_4543_cov_4.447415, whole genome shotgun sequence.
ACCESSION   DAOMXO010000102 DAOMXO010000000
VERSION     DAOMXO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10206_length_4543_cov_4.447415"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..2273)
                     /locus_tag="PLH57_03190"
     CDS             complement(6..2273)
                     /locus_tag="PLH57_03190"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRK01643.1"
                     /translation="MSARPQVETTPAMVTKTYEMLKKNLPTLRKRLNKPLSLAEKVLL
                     GHLDAPETQELKPGSSILRLRPDRVTMQDATAQMALLQFMQSGRESVAVPSSVHCDHL
                     IRAHKGSAADQKTAESENREVYDFLRSSCAKYGIGFWGPGAGIIHQVVLEQYAYPGCL
                     VIGTDSHTPNGGGLAMAAIGVGGADAAETMSGLAWEVKYPKRIGVKLTGKMSGWTAPK
                     DVILKVCELLTTKGGTDAIIEYFGDGCASISCTGKATITNMGAELGATTSMFPYDERM
                     HRYLKSTGREDLAKLADAAKDLLTADPEVLASPEKYFDKVLEIDLSTLEPHVVGPHTP
                     DLARPVSKMAEDVKKNNYPAKLTSALIGSCTNSSYEDMGRAADVAKQAQKAGATSSVP
                     FMISPGSYQIFETIKRDGQMKELESIGGLVLSNACGPCIGQWKRDDIKAGEKNSIITS
                     FNRNFPARNDANPETMAFIGSPEVVVAFALAGRLDVNPLKDEITTASGKKLKLQPPEI
                     ADEIPSKGFVSSKEGYVAPPSTGASVQIEVSPKSDRIQLLSPFPKWDGNDFKEFPLLL
                     KAKGKCTTDHISPAGPWLKYRGHLDNISNNMFTGAVNAFTGEAGKVNDGTGSSKPTPE
                     VARALKAQGLRWIVVGDENYGEGSSREHAAMSPRWLGAAAVITRSFARIHETNLKKQG
                     VLPFTFVDAKDYDKVQEGDKISILGLSGLAPAKNVECVLKHLDGREDKISLKHSLTEE
                     QIAWFKAGSALAANK"
     gene            complement(2348..2902)
                     /locus_tag="PLH57_03195"
     CDS             complement(2348..2902)
                     /locus_tag="PLH57_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01644.1"
                     /translation="MSQENPNILDLTLPQTLYELAIDDHEIYGPAGAKLIADLRERAF
                     EADYTHLYFRDPMRPGIVWFMALGSSGSLPTRGKLDYCRKITDKVFPGFLAKVDSDSD
                     DNIMTQIPPLTTGVSGFELATALEYLVFVYPSMVRAWAALVFTYGEMLGMREAAETVR
                     NECNTLCPYAQVVAYLTTSVATEV"
     gene            complement(2973..3950)
                     /locus_tag="PLH57_03200"
     CDS             complement(2973..3950)
                     /locus_tag="PLH57_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01645.1"
                     /translation="MSIFLSLVGGASHADASDEVEKITECGEYLAASVNSGSHFGSKA
                     DEEYALNIALNSARGLAIRLKALEHLAAIREQRAEWAMDAGRPSFAYVLVDPHGLRAK
                     FLESAQALPQLRDAAAVVLGFYNSDHYRQARDAAKRALSIADNVTSLQDLLDLSTADR
                     ALLSEMHRYRALESEALEFIEHAVRIFKMWGVRSLPEIATAPFRRPVESFFQSAVHRI
                     AQRASAVVYRPLNRMDLLEDLVQTNLEGTKIATSGVQLLEGGTDSLEHIVRILDTLEH
                     SNARMHRDEANSLRIWGGAVLNTPEISMLFKQRVRRLMKVFQVNIPANG"
     gene            complement(4036..>4543)
                     /locus_tag="PLH57_03205"
     CDS             complement(4036..>4543)
                     /locus_tag="PLH57_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRK01646.1"
                     /translation="EQRARAVNVPRRRIVDDDVLIDLSRTRPQDLEHLQAFRGLNKGE
                     FRTSGDAILQAIARGLANAQAGRAVPPIRQEPASDGEARVLELVICMARAIADQNAIS
                     LKHLMSADWALEIIRARPRTVDELAALNLLSQGAVELLGRQLTDLLEGRLSLSIRDGA
                     VEIFRVRS"
BASE COUNT         1053 a         1241 c         1085 g         1164 t
ORIGIN      
        1 aatctctatt tattggctgc tagagcggat cctgccttaa accaagcgat ttgttcttcg
       61 gtcagcgagt gtttaaggga gattttatct tcgcgtccgt cgaggtgttt caacacacac
      121 tccacatttt tcgccggagc aagacccgag agcccaagaa tgctaatttt atcgccctcc
      181 tgaaccttgt cgtaatcttt ggcgtcaacg aacgtgaaag gaagtacgcc ctgttttttt
      241 aaattggttt cgtgaattct cgcaaacgag cgagtgataa cagctgccgc ccctaaccat
      301 cgtggcgaca tcgctgcgtg ctcgcggctt gagccctctc catagttttc gtcgccaacc
      361 acgatccacc gaagaccttg ggccttgagt gcacgcgcca cttctggcgt gggttttgaa
      421 cttcccgttc cgtcgtttac ttttccagct tctcccgtaa aggcattcac ggcaccggta
      481 aacatgttgt ttgaaatgtt atccaaatgc ccacgatact tcagccatgg tcccgccgga
      541 gaaatatgat ctgtagtaca cttgcccttt gccttgagca gcagcggaaa ttctttaaaa
      601 tcattaccat cccacttcgg aaacggcgaa agcagctgaa tgcgatcgct ctttggtgac
      661 acttcaatct gaactgaagc cccagtcgaa ggtggtgcta catagccctc tttgctgcta
      721 acgaacccct tactcggaat ttcatcggct atttctggcg gctgaagttt taactttttc
      781 ccactagccg tcgtaatttc atctttaagc ggattgacgt ccaatcgccc tgccaaagca
      841 aatgcgacca ccacttcggg actgcctatg aacgccatgg tttctggatt agcgtcgttt
      901 cgcgcaggaa aattgcggtt aaacgacgtg ataatagaat tcttttcacc ggctttaatg
      961 tcgtcacgct tccattgccc aatgcatggg ccacaagcat ttgaaagcac aaggccccca
     1021 atagattcca actctttcat ctggccgtcg cgcttgatgg tttcaaaaat ttgatacgaa
     1081 ccaggagaaa tcataaacgg caccgaagaa gtcgcccctg ctttctgcgc ttgtttcgca
     1141 acgtcagccg cacgtcccat atcttcataa gaagaattgg tacatgaacc aatcagtgcc
     1201 gaagtaagct tggcgggata gttatttttc ttaacatctt ctgccatttt cgaaacgggt
     1261 ctcgctagat ctggcgtgtg tggccccacc acgtgcggct cgagcgtcga gagatctatt
     1321 tctaacactt tatcgaaata tttttcaggt gacgccaaaa cctcgggatc cgccgtgagc
     1381 agatcttttg cggcgtcagc tagtttcgct agatcttcgc gaccagtgga ttttaaatac
     1441 cggtgcatgc gttcgtcata tgggaacatc gacgtcgtcg ctccgagttc tgcgcccatg
     1501 ttggtgatcg tcgctttgcc ggtgcaacta atgctcgcgc atccgtcacc aaaatattcg
     1561 atgatcgcgt ctgttccgcc tttggtggtg agtagttcgc aaacttttag gatcacgtct
     1621 ttgggcgcgg tccacccact catcttcccc gtgagtttaa cgccaatgcg ttttggatac
     1681 ttcacttccc aagccagtcc cgacatcgtt tcagcagcgt cggccccgcc cactccgatg
     1741 gctgccatcg caaggccgcc gccatttggc gtgtgactgt ccgtgcctat caccagacat
     1801 ccggggtagg catattgctc gagcactact tggtgaataa taccggcgcc tggtccccaa
     1861 aaaccaattc catatttcgc acacgaagag cgcagaaaat cgtacacttc ccgattttcg
     1921 gattcagccg ttttttggtc agccgctgaa cctttgtgcg ctcgaatcaa atgatcgcaa
     1981 tgaaccgacg aaggtactgc gacgctctca cgccccgatt gcatgaattg cagaagcgcc
     2041 atttgcgcag tggcgtcttg cattgtgacg cggtcgggcc gcaatcggag aattgaagag
     2101 ccaggtttta gttcttgggt ttcaggggca tcgagatgac cgagcagcac tttttccgct
     2161 aggctcaatg gtttattcag tcgctttcga agcgttggta agttcttttt caacatttcg
     2221 taagttttgg tgaccattgc tggtgtagtt tcgacttgcg ggcgtgcgct catgaggaag
     2281 gcctcctgtg ttattcttcc aatttcgcgc agttagacca aggtttcaag cgggaatagc
     2341 gaacaaacta tacttcggta gcaactgaag ttgtaaggta tgcgacaacc tgcgcatagg
     2401 ggcagagggt gttgcactcg ttgcgcactg tttcggcggc ctcgcgcatt ccgagcattt
     2461 cgccataggt aaaaaccaag gcagcccagg cgcgaaccat cgacgggtac acgaaaacta
     2521 aatattctaa cgcggtggcc aattcaaacc cactaacgcc ggtagtgagc ggcggaatct
     2581 gagtcataat gttgtcgtcg gaatcgctat cgactttcgc caaaaatccc gggaacactt
     2641 tatccgtaat ttttcggcag tagtccaact tacctcgcgt cggtaaactt ccactcgacc
     2701 cgagagccat gaaccaaaca atgcccggcc gcatggggtc acgaaagtaa agatgagtgt
     2761 agtctgcttc aaacgcgcgc tcgcgcaaat cggcaatgag ttttgctcct gcagggccgt
     2821 aaatttcgtg atcatcgatc gctaattcat aaagcgtctg cggcaatgtt aggtctaaga
     2881 tgtttgggtt ttcttgcgac atggagttga ttttatcgca atcttcaacg ggcgcgagag
     2941 gctgattttt cctctctaaa gctgggcact gattacccgt tcgcaggaat gtttacctga
     3001 aaaaccttca tcaatcggcg cacgcgttgc ttaaaaagca tggaaatctc aggagtatta
     3061 agcacggccc ctccccaaat gcgcaaacta ttggcttcgt cacgatgcat tcttgcgttc
     3121 gaatgctcta acgtatcgag tatcctaact atgtgttcga gggaatctgt ccccccctcg
     3181 agtaattgaa ctccggaggt cgcaatcttg gtgccttcga gatttgtctg aaccaagtcc
     3241 tccaacaaat ccatgcgatt gaggggccgg tacaccactg cgctcgcacg ttgagcaatc
     3301 cgatgaaccg cgctttgaaa aaaactttcg accggtcggc gaaacggcgc ggtcgcgatt
     3361 tctggcaatg atcgcactcc ccacatctta aaaattcgaa cagcgtgttc gataaactca
     3421 agcgcctcgc tttcgagcgc ccggtaccgg tgcatttccg atagcaatgc gcgatcggct
     3481 gttgataaat ccaatagatc ttggagactc gtgacgttat cagcaatact caaggcccgt
     3541 tttgcggcgt ccctagcttg ccgatagtgg tcgctattgt agaaccctaa aaccaccgcc
     3601 gccgcatctc gcaattgagg tagcgcctgc gcactttcta aaaactttgc tcttaaaccg
     3661 tgagggtcca ccaaaacgta ggcgaaactc gggcgcccgg cgtccatcgc ccattctgca
     3721 cgctgttccc gaatggccgc caaatgttcg agtgctttta accggatcgc aagaccacga
     3781 gcggagttta acgctatatt caacgcatac tcttcgtctg ctttggagcc aaagtggctg
     3841 ccagaattca cgctcgcagc gaggtattca ccacactcgg tgatcttttc gacttcgtcg
     3901 ctcgcgtccg catggctcgc cccaccgaca aggctcaaaa aaatgctcat tgtataataa
     3961 actcgccaca taatgatggg aagcccagta ccataaaaca gacggatttt gtctattatt
     4021 ttttaaacta cccgttcagg atcgaacgcg aaatatctcg actgccccgt ctcggatcga
     4081 gagcgataac cgcccctcaa ggaggtctgt gagttgacgt cccaatagtt cgacggcacc
     4141 ctgcgataac aggttcaatg ccgctagttc gtcgactgta cggggacgcg cgcgtatgat
     4201 ttccagggcc caatccgcac tcattaagtg tttaagtgaa attgcattct ggtccgcaat
     4261 cgcgcgggcc atacaaatca ccaattccag tacgcgagcc tccccatctg aggcaggttc
     4321 ctgacgaatc ggcggtaccg ctcggcctgc ctgggcattg gccaaacccc gggcaatggc
     4381 ctgcaaaatt gcatctccgc tagttcgaaa ctcacccttg ttaagcccac gaaaggcctg
     4441 caggtgttct aaatcttgag gacgagtccg cgaaagatca atgagtacat catcgtccac
     4501 gatgcgtcgt cgcggcacgt tcaccgccct agcgcgttgt tct
//
LOCUS       DAOMXO010000103         4543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10207_length_4543_cov_4.237299, whole genome shotgun sequence.
ACCESSION   DAOMXO010000103 DAOMXO010000000
VERSION     DAOMXO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10207_length_4543_cov_4.237299"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PLH57_03210"
     CDS             <1..882
                     /locus_tag="PLH57_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK01647.1"
                     /translation="FRNPEILDVFDRALKKGQTVPVKPVAIELKEGRRYFSISVSPLR
                     RESDGIYGAVGIFHDVTELKTAEQMRIDFVANVSHELRTPLTSIKGFTDTIIDDIRSQ
                     RPVENAFLEKIAKNSDRLLNLINDLLDLSSLDAADDRADLLHRGKVGTREITERILHG
                     LKDSFAKKRQKISTEFDAGMVTADSKRLEQVLINLLENAYKYAPDEGEIAVAWKKDGE
                     DVLLRVYNSGPGIPREHHPRLFERFYRVDKGRSREMGGTGLGLAIVKHILQRHGGTVW
                     VESKEGRGATFVCKFPG"
     gene            complement(901..1854)
                     /gene="add"
                     /locus_tag="PLH57_03215"
     CDS             complement(901..1854)
                     /gene="add"
                     /locus_tag="PLH57_03215"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRK01648.1"
                     /translation="MLTTELHRHLDASLRPDTLAELIRAQGVHPTLRTTEDIKEKFWI
                     TKPMGSLKEVLDCFVLFQKVLTTETILERVARETIEDVAREGIKTVELRYSPSFVSEY
                     SQLDWRLALGAIERGISMGSKATGVQARLICIISRDYGSNTADKTVDFAVAHKESFVA
                     LDLAGNEVDYPNPIFAGALRRARDAGFSITVHSGEATGPETVWQAIDELGAQRIGHGI
                     HSIKDPELVKRLVRDRIHLEVCPTSNVNTRSVASWGEHPLRRLFDAGVRVSINTDDPG
                     IFGVGLDEEVARAKKAFGFSEPELATINSMAFEDLFKVREI"
     gene            1912..3591
                     /locus_tag="PLH57_03220"
     CDS             1912..3591
                     /locus_tag="PLH57_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK01649.1"
                     /translation="MNRRVLQFFLIMWAVTLPAVAEVTRGRGTPVFSQDRYGEERIEA
                     ATPEYTQDQFAEPDIKVDLSETLRDVPERGREWSEVEIAKGIVQLNAKRFDSARDHFE
                     RALRKDERNFVAREYLAAISEAQGNYDAAVEQLARAKHIAPESRHGVLNFRIGFIYFR
                     QGQVDRSRAYLLKALEQKAVATHAYYLLGQMSFREKNYVEAEKYFHESALLSRRFGAR
                     AAERELTQATYYYLGEVYARLGYFDSASTSLARAEPGESAEIRNAAWRTHSEVNQARW
                     TAGAGLFGQYDSNVLVLPVDALLPDEISSQSGNSAVFTAFGDWTSPRYREWSVNAGTE
                     FYWKTHLDSALRAYDVLNFHFDGGVSRYSGEDWRVDAKYSFDRTLADRLDWYTYQTTH
                     GPDLSVQFTPHQRWKWTAGFFYLISKFGRDPLAGIEQRSGRTTGVYLKAAIDAPNPRL
                     LPNFKYQFEHVAARGLNYRSDVHSLLAGVDWRWFERTRMHASVGVSMLVFPGHAGDRS
                     DILRTGTIGVLHWFEPHLAGLIDVSVRQNDSNAAGLDYNRAAVTVGMTYRL"
     gene            complement(3586..4530)
                     /locus_tag="PLH57_03225"
     CDS             complement(3586..4530)
                     /locus_tag="PLH57_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HRK01650.1"
                     /translation="MQKTVLLTEAKAARERIAPFVTSTALQLSEPLSSRTGKNIWLKL
                     ENQQLTGSFKIRPALNGILCHLAQARNQGVITTSSGNYAQAVAYAAKLLGVHATIVMT
                     PSTATIKIEKTKAHGARVVFCEDHFESRFETLERIQKQEGQLVLHGFDSSETIAGNGT
                     IALELVDQLKVPFSLFSPASGGGLVSGIAGTLKALDPVYEIFGAQPEVGGAIVRSLAR
                     GSPVNVGPFKTIADALVASVPGARTFPIIQAHCANFFGVTEESITHCFENLLSAGEIG
                     PLEPGGAVSVAAMLEHFNSCLHRDIVCVISGGNFTRSL"
BASE COUNT         1124 a         1078 c         1164 g         1177 t
ORIGIN      
        1 tttagaaatc ctgaaatttt ggacgtattt gaccgcgcgc taaaaaaggg tcaaaccgtc
       61 ccagttaaac ccgtagctat cgaactcaaa gaggggcgtc gttacttttc gatttctgtg
      121 tctcccttgc gtcgcgaaag tgatggcatt tatggcgcag taggaatatt tcacgatgtt
      181 acggaactta aaactgcgga gcaaatgcgt attgatttcg tcgctaacgt ttcgcatgaa
      241 ttgcgcactc cgttgacctc tattaagggg tttaccgata ccattattga tgacattcgc
      301 agtcaacgcc ccgttgaaaa tgcttttttg gaaaaaatag caaaaaattc tgatcgactt
      361 ttaaatttga tcaacgattt actcgatctt tcgtcgctcg atgcggccga cgatcgcgca
      421 gatcttttgc atcgtgggaa ggtcgggacc cgagagatca cggaacgtat acttcacgga
      481 ttaaaagact cctttgccaa gaaaaggcaa aaaatttcaa cggagttcga tgccggcatg
      541 gttacggccg attcgaagcg actagaacag gtgctcatca atcttttaga aaacgcctac
      601 aaatacgcgc cagacgaagg cgaaatcgcg gtggcctgga aaaaagatgg agaggatgtt
      661 ttgctccgag tttataattc cggacccggt attccgcgtg aacaccatcc gcgccttttt
      721 gaacgatttt atcgggtcga caaggggcgc tcccgcgaaa tgggtggcac cggcctaggc
      781 ctcgcaattg tgaagcatat ccttcagcgg catggtggta ctgtttgggt cgaaagcaag
      841 gagggtcggg gcgcgacctt cgtttgcaag ttcccaggct agtggctgaa catttgcgga
      901 ttaaatctcg cgcactttga acaaatcctc gaacgccatc gaattaatgg tagcgagttc
      961 tggctctgag aaaccaaatg cttttttggc tcgagcgact tcttcatcga gtccgacccc
     1021 aaatattcct gggtcatcag tgttaattga aactcgaact cccgcatcaa acaatcggcg
     1081 cagcggatgc tcgccccaac tagcgactga acgcgtgttt acattgctcg ttggacatac
     1141 ttcgaggtga atgcgatcac gcaccaatcg tttaacgagc tcgggatctt ttattgaatg
     1201 aatgccgtgt cctatgcgct gcgctccgag ttcgtcgatt gcttgccaaa cggtttcagg
     1261 tcccgtggct tcgccagaat gaacagtaat cgaaaaccca gcgtctcggg ctctgcgaag
     1321 cgcacccgcg aaaatcggat tcggatagtc tacttcgttt ccagccaaat ctagcgccac
     1381 gaaagattcc ttgtgggcga cagcgaaatc tactgtttta tctgcagtgt tcgagccgta
     1441 gtcgcgacta ataatgcaaa tgagcctcgc ttgcacgccg gtagctttgc tacccataga
     1501 aatgccacgt tcaatcgccc ccagggccaa gcgccagtct agttgggagt actctgaaac
     1561 gaaactgggg gaatatcgca attcaacagt tttaatcccc tctcgtgcga catcttcaat
     1621 cgtttcacga gcgacacgct ctaaaatagt ttcagtcgtg agcactttct gaaaaagtac
     1681 gaaacaatcg agtacttcct ttaaggagcc catcggcttt gttatccaga atttttcttt
     1741 gatgtcctca gtagtccgaa gcgtggggtg aacgccctga gctcgaatca gttccgcgag
     1801 agtatctggt cgtaacgaag cgtcaagatg tcggtgtaat tccgtagtta acatttcgag
     1861 aaccttattt tcttttagag tgaacgacaa tagactttaa gggtgacccc attgaataga
     1921 cgagtacttc aatttttttt gattatgtgg gcagtgactc tgcctgccgt ggcagaggtt
     1981 acccgtggaa gagggacacc cgttttttct caagaccgtt acggcgaaga acgaatagag
     2041 gctgccacac cggagtatac ccaggatcaa tttgctgagc ccgatatcaa agtagacctt
     2101 tctgaaacct tgcgtgatgt gcccgaaagg ggccgtgagt ggagtgaagt tgaaatcgca
     2161 aaaggaattg ttcaactaaa tgctaaacga tttgattcag cgcgcgatca ttttgaacgg
     2221 gcccttcgca aggacgaacg aaattttgtg gcgcgagaat atttggctgc gatttctgag
     2281 gcccagggaa attacgatgc cgcagtggaa caactcgcgc gggctaaaca catcgcgcca
     2341 gaatcacgtc acggggttct aaattttcgc attggattta tttattttcg ccaaggacaa
     2401 gtcgatcggt cgcgagcgta tttgctcaag gcgttagaac aaaaagctgt agctactcac
     2461 gcttattatt tgctcggaca aatgagcttc cgagaaaaaa attatgtcga agcagagaaa
     2521 tattttcatg agtccgcgct cctcagtagg aggttcggtg ccagagcagc tgaacgagag
     2581 ctgactcagg caacctacta ttatttgggc gaagtttatg ctcgtttagg atatttcgac
     2641 agcgcatcta cgagtttagc tcgcgcggaa ccaggcgaga gtgcggaaat tcgcaatgcc
     2701 gcttggcgta ctcattccga agtcaatcaa gctaggtgga ccgcgggggc gggacttttc
     2761 ggtcagtacg attctaatgt tttggtgctt cccgtggacg cactattgcc cgatgaaata
     2821 tctagtcaat ctggcaattc tgcagttttt accgcgtttg gtgattggac ttcgccccgc
     2881 taccgggagt ggtctgttaa cgccggtaca gagttctatt ggaaaactca cctcgactcg
     2941 gctctacgag cttacgacgt attgaatttt catttcgacg gcggggtgtc gcgctacagt
     3001 ggcgaagatt ggcgagtcga cgcgaaatat agcttcgacc gaaccctggc agatcgactc
     3061 gactggtaca cctaccaaac aactcatggt ccagatcttt cggttcagtt caccccccat
     3121 caaagatgga aatggaccgc tggctttttt tatctgattt cgaaattcgg ccgagaccca
     3181 ctggctggaa tagagcaacg ctctggccgc acgacaggtg tttatcttaa agccgccatt
     3241 gatgcgccca atcctcggct actgcctaat tttaagtacc aattcgaaca cgtagctgct
     3301 cgcggactta attaccgttc agacgtacat tcgttgctcg ccggggtaga ttggcgatgg
     3361 tttgagcgaa ctcgcatgca cgcgagcgta ggtgtttcga tgctggtgtt tccgggccat
     3421 gctggcgacc gttcagacat tttgagaact ggaacaattg gtgttttgca ttggttcgaa
     3481 cctcatttag caggactcat tgacgttagc gttcggcaaa atgattcgaa tgccgctggt
     3541 cttgactaca atcgtgctgc ggtgacagtc ggaatgactt accgcctata aagagcgggt
     3601 aaaattgcca ccgctaatca cgcagacaat gtcgcggtga agacagctgt taaaatgttc
     3661 aagcatggcc gctacgctca ctgcgccgcc tggttcgagc ggaccgattt cgccggcgct
     3721 taacaaattt tcaaaacagt gagtgatgga ttcctcggtc acaccaaaaa agttggcaca
     3781 gtgagcctga ataattggga aggtgcgcgc tcccggaacc gaagctacaa gtgcgtcggc
     3841 gattgtcttg aaaggaccta cgtttactgg ggagccgcgt gccaaggaac gtacgatggc
     3901 acctcccact tctggttggg ctccaaaaat ttcgtagacg ggatctaaag ccttcaaagt
     3961 tcccgcaatc ccggaaacca gaccaccgcc actggcgggg ctgaacagag aaaagggaac
     4021 ttttagctga tctaccaact ctagtgcaat ggttccattc ccggcaattg tctcgctgga
     4081 atcaaatccg tgtaaaacca attgtccttc ttgtttttgg attctttcga gcgtttcaaa
     4141 acgactttcg aagtgatctt cacaaaatac cacgcgtgcg ccatgggctt tggttttttc
     4201 aatttttatt gtggcagtcg aaggggtcat gacgatggtg gcgtgcaccc cgagcagttt
     4261 agctgcgtag gctacggctt gagcatagtt tcccgacgac gtggtaatga cgccctggtt
     4321 tctggcttgg gctaaatgac agagaatacc attaagggcc ggacgtattt tgaaggagcc
     4381 ggtaagttgc tggttctcga gttttaacca aatgtttttt ccggttctcg aactcaaggg
     4441 ttccgaaagc tgcaaggccg tagaggtgac gaacggtgct atgcgttcac gggcggcttt
     4501 ggcctcggtc aataagactg ttttttgcat gagttagttt tag
//
LOCUS       DAOMXO010000104         4533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10240_length_4533_cov_5.163912, whole genome shotgun sequence.
ACCESSION   DAOMXO010000104 DAOMXO010000000
VERSION     DAOMXO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10240_length_4533_cov_5.163912"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLH57_03230"
     CDS             complement(<1..911)
                     /locus_tag="PLH57_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01651.1"
                     /translation="MYTLAPNTMAKESDKNNNSGTELEYIPVDVSQLRVGTLVDRPLY
                     ARMESRNKFILMARSLHPLEQKTLDRFLRFGHVYSIFPALDERYGKLAMSVKRVEELL
                     FDGSLAPFEKTSLIRKDTEWLSDLVMKKNGDADVAVFFLNKLLHVPHPQTIDYVVEHS
                     VDLHEHSLRVASVATLLLLWTEAPKEEFLKDFLTSIYCEELSVYRDDSPNLRLIAMAT
                     AGYTAQDSDRAYSFELAKKGEIPGISNLDRRVVETLSRRGKMIQYGDELAEIVELARW
                     ICGGTGVQFRVLWESRLVRKLSRLMNFS"
     gene            complement(949..1953)
                     /locus_tag="PLH57_03235"
     CDS             complement(949..1953)
                     /locus_tag="PLH57_03235"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRK01652.1"
                     /translation="MAAPLIEVKNLVKRFPIKGGIFSREIANVHAVSDVSIAIHRGET
                     LGLVGESGCGKSTLGRCILRLLEPTAGTIFYDGADITRLGTREMRRLRRKMQIIFQDP
                     YASLNPRMTVGDIISEPLSIHGLYKGEKDKRVKTLLDLCGLRSEAMFRYPHEFSGGQR
                     QRICIARALAVEPEFIVCDEPVSALDVSIQSQILNLMMDLQKEFKLTYLFISHDLKVV
                     EHMSDRVAVMYLGKIVEQAEAKEIYTRPQHPYTRALLSAIPIPDPNFKKERLILEGDV
                     PSPISPPNGCHFHPRCPNVKENCRQEYPALQFRPGTSDQAGKSDHLAACFYAGQRGSL
                     "
     gene            complement(1938..2960)
                     /locus_tag="PLH57_03240"
     CDS             complement(1938..2960)
                     /locus_tag="PLH57_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK01653.1"
                     /translation="METILEVKNLRTEFVTEAGTVCAVDDVSFAVKKGKTIGIVGESG
                     CGKSVTSLSIMRLIPTPPGRISSGQILYKGKDLLQLPLSEMRKIRGNEISMIFQEPMT
                     SLNPVFTVGDQIVEAVRLHQNVSRADARARACEMLKLVGISSPESRLDDYPHQLSGGM
                     RQRVMIAMALSCNPDLLIADEPTTALDVTIQAQILDLMRRLRETFGMAMILITHDLGI
                     IAETADEVLVMYAGKVVEQGSVAEIFGEPLHPYTRGLLNSVPVFKPVSATSGERDSSQ
                     QKKKLDTIPGIVPSLLELPKGCRFQTRCTFATEECKTAEPELRSVRGDSHRAACIKDL
                     APWQRR"
     gene            complement(2962..3837)
                     /locus_tag="PLH57_03245"
     CDS             complement(2962..3837)
                     /locus_tag="PLH57_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK01654.1"
                     /translation="MIRLPSQSKSLWQDAWRRIRRDRLALWALGVIVLYSLVALCSKF
                     GLLASTWDAEVGGPYASPSFENFKTIFGTDIFGRSVLYKVIHGTRVAMSVGLMTSLIA
                     IPIGVTLGAFGGYFGGKVDDVIVWFYTTLSSIPGIMLLIAIAYILGKGLFSVYIALGI
                     TSWVSLARLIRGEFMKHKSREYVIAAEAIGAGHRIRIFRHILPNVLHIVIINMSLQFQ
                     IAIKSEVILSYLGLGVQGQPSWGVMIDDAKLELARGVWWQLTAATFAMFFVILAFNIL
                     GDALRDAFDPKLRKG"
     gene            complement(3834..>4533)
                     /locus_tag="PLH57_03250"
     CDS             complement(3834..>4533)
                     /locus_tag="PLH57_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK01655.1"
                     /translation="SYTTKQQITDMIWRGMPASLSLAIPAFIVEILLGLAIALLVSFY
                     RGTFFDRAVVVLCVMGMSVSALAYILFGQYVFAYWLGLFPISGYDTDILYRFKYIALP
                     AIIWVVVSLGINVRVFRTFVLDEVNQDYVRTARAKGQSEKKILFHHVLKNALIPVLTY
                     VVIQIPVLITGSFLLENFFGIPGLGSITIDAIHNSDFPVIKAMTTIETLLFMVFALAT
                     DLLYSVVDPRVRMR"
BASE COUNT         1256 a         1167 c          979 g         1131 t
ORIGIN      
        1 gaaaaattca taagccgact gagctttcgc actaggcgac tttcccaaag tacgcgaaac
       61 tgcaccccgg tgccaccaca gatccaccga gccaactcta caatttctgc gagctcgtcg
      121 ccgtactgaa tcatttttcc acgacgacta agagtctcta ctacccgacg atcgagattc
      181 gatattcctg gaatttcccc ttttttagcc aactcgaaag agtaggcccg atcgctgtct
      241 tgagccgtgt atcccgcagt cgccatagcg atgagtcgta ggttaggact atcatcacgg
      301 taaacgctca attcttcgca atagatcgac gtcaaaaaat cttttaggaa ctcttctttg
      361 ggagcttcag tccatagcaa aagtaaagtc gccacagagg ctactcttaa gctgtgttcg
      421 tgtaagtcga ccgagtgttc aacgacatag tcaatggtct gagggtgggg aacgtgcaac
      481 aacttattca agaaaaagac cgctacatcg gcgtccccgt ttttcttcat gacaagatca
      541 gaaagccact cagtgtcttt gcgaatcaaa ctggtttttt caaacggcgc tagagaccca
      601 tcgaatagca gttcctctac tcgtttcact gacatcgcca actttccgta acgctcgtcg
      661 agtgccggaa aaattgaata tacgtgcccg aatcgtaaaa aacgatctaa ggttttttgt
      721 tcgagcgggt gcaaagatct cgccattaaa ataaatttgt ttcgcgactc catgcgcgca
      781 tacagcggcc ggtccacgag cgtacccacc cgcaactgac ttacgtcgac cggaatatat
      841 tccaactccg tgccggagtt attatttttg tcgctctctt tggccatagt attaggcgcg
      901 agtgtataca cccgctcctc ttctcgtatc ggaaaattcg cttttttatc aaagagatcc
      961 gcgctgtccg gcgtagaaac aggcggcaag atgatcagat ttgcccgcct ggtcgctggt
     1021 tcctggtcgg aattgcaggg caggatattc ctgtcggcaa ttttccttca cgttgggaca
     1081 tcgcgggtga aaatggcagc catttggtgg ggaaatagga ctaggaacat cgccctctaa
     1141 gattaggcgt tcctttttga agtttggatc aggaatcgga atagcactca gcaaagctcg
     1201 cgtgtagggg tgctgtggcc tggtgtaaat ttccttcgcc tcagcttgtt cgacaatttt
     1261 tccaagatac atgacggcaa ctcgatcgct catatgttca actaccttga ggtcatgtga
     1321 aataaacaag taggttaact tgaactcctt ttgcagatcc atcatgagat ttaatatctg
     1381 agattgaatc gaaacatcca gcgcgcttac cggttcatcg caaacgataa attctggctc
     1441 taccgctaag gctcgcgcta tacatattcg ctggcgttga ccgccggaaa actcgtgggg
     1501 ataacgaaac atagcttcgg agcgcaaacc acagagatcc aacaaagttt ttacgcgttt
     1561 atctttttcc cctttataga gtccgtgtat cgatagaggt tctgaaatga tatctcccac
     1621 tgtcattcgt ggattaagcg aagcgtaggg gtcttgaaat ataatctgca tttttcggcg
     1681 aagtcttcgc atttcgcgag ttccgagccg ggtgatatcc gcaccgtcat aaaatattgt
     1741 gccagcggtc ggttctagta accgtaaaat acatcgacca agggtagatt ttccgcaacc
     1801 gctttcgccc acaagaccca aagtctctcc tcggtgaatc gcgatactga cgtcggaaac
     1861 agcgtggacg tttgcaattt cacgggagaa aattccgcct tttattggaa aacgttttac
     1921 aagattttta acttcaatca acggcgctgc catggtgcta aatcctttat acaagcggcc
     1981 ctgtgactat caccccgtac cgaacgaagt tcgggttccg cggttttgca ttcttccgtg
     2041 gcaaacgtac agcgggtttg gaaacggcaa cctttcggaa gctcaagcag acttggcact
     2101 atgccaggaa tcgtatccaa tttttttttc tgctgtgacg aatcccgttc ccccgaagtc
     2161 gcagaaacgg gtttaaagac cggaacgctg tttaaaagtc cgcgcgtata gggatgaagt
     2221 ggttcgccaa aaatttcggc gacacttccc tgctctacca ccttaccagc gtacatgacc
     2281 aatacctcgt cggcggtttc ggctatgatt ccgagatcgt gggtaataag aatcatggcc
     2341 atgccaaatg tttcccttaa ccgccgcatc aggtctaaaa tttgagcttg aatcgttaca
     2401 tcgagcgccg tggtcggttc gtcggcaatc aacaagtctg gattacacga aagcgccatt
     2461 gcgatcatca ctcgttgacg cattccacca ctaagctgat gcggataatc gtccaaacga
     2521 ctttccggcg aagaaatgcc cacaagtttt agcatttcac aagcgcgagc tcgagcatca
     2581 gctcgactca cgttctggtg caatcgaact gcttcgacta tctgatcacc aactgtaaaa
     2641 actggattaa gcgacgtcat gggttcttga aaaatcattg aaatttcgtt gccgcgaatt
     2701 tttcgcatct ccgaaagtgg cagttgcaat aaatctttgc ctttatacaa aatttgccct
     2761 gacgaaattc gacctggcgg cgtaggaatc aaacgcatga tcgacaacga cgttacgctc
     2821 ttgccacaac cactttcacc tacgatacca attgttttgc cttttttgac ggcgaaactt
     2881 acatcgtcga ccgcacatac tgtaccagct tcggtaacaa attcagttcg aagatttttt
     2941 acttccagta tggtttccat ttcaaccttt tcgcagttta ggatcaaaag cgtcgcgcag
     3001 cgcatcgccc aatatattga aggccaaaat tacaaaaaac atggcaaacg tcgctgccgt
     3061 aagctgccac cacactccac gcgcaagttc cagtttcgca tcgtcaatca tcactcccca
     3121 actaggctgt ccctgcacac ctaatcctag gtacgaaagt ataacctcgg atttaatagc
     3181 aatctgaaac tgcagtgaca tgttgatgat cacaatgtgc aatacattgg gcagtatatg
     3241 ccgaaaaatt cgaatccggt gcccggcccc tatcgcttca gcagcaatca catattccct
     3301 ggatttgtgt ttcatgaact cgccgcggat caaacgagca agactcaccc agcttgtaat
     3361 gcccagtgcg atgtaaaccg aaaacagacc cttgcccaga atgtaagcga ttgcgattaa
     3421 caacattatt ccgggaatcg acgaaagtgt ggtgtaaaac cagacaatca catcgtcgac
     3481 tttgccacca aaatagccgc cgaatgcacc cagcgtaacc cctatcggta tcgcaatcaa
     3541 cgaagtcatt aggcccacgc tcatagcaac ccgcgtgccg tgaataacct tatagagcac
     3601 gctccgccca aaaatatcgg tgccgaatat agttttaaaa ttttcgaaac tcggcgatgc
     3661 ataaggtccg cctacttcgg cgtcccaagt cgaagcgaga agtccaaact tagaacacag
     3721 cgccacgagt gagtagagta cgataactcc caaagcccac aaagctagtc tatcacgacg
     3781 aatcctacgc caagcatctt gccacaacga cttactctgg ctgggtaacc ggatcatcgc
     3841 atcctcactc tggggtcgac tacgctgtaa agcaagtcgg tggccaacgc aaaaaccata
     3901 aaaagtagcg tttcgattgt cgtcattgct ttaatcacag gaaaatcaga gttatgaatt
     3961 gcatcaatcg taatgctccc caacccggga attccgaaaa agttttcaag caaaaacgaa
     4021 cccgtaatca acaccggaat ttgaatcaca acgtaagtaa gcacggggat caaagcattt
     4081 ttcagtacat gatggaatag gattttcttt tcggactgcc cctttgccct ggcagttcgc
     4141 acatagtcct gatttacctc gtcgagtaca aaggtacgaa agaccctcac attaattcct
     4201 agactcacca ccacccaaat aatcgcaggc aaagcgatgt acttaaatcg gtagagaata
     4261 tctgtgtcgt accctgaaat cggaaatagc cccagccaat acgcaaaaac atattgaccg
     4321 aataaaatgt aagcaagcgc ggacacgctc attcccatta cgcaaagtac aaccaccgcg
     4381 cgatcgaaaa aggttccgcg ataaaacgaa acaagtaacg caatggctaa accaagcagt
     4441 atttccacaa tgaaggcagg aatcgccagc gacagcgagg ctggcatacc tcgccaaatc
     4501 atatcggtaa tttgttgttt agtggtgtag cta
//
LOCUS       DAOMXO010000105         4517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10303_length_4517_cov_5.092784, whole genome shotgun sequence.
ACCESSION   DAOMXO010000105 DAOMXO010000000
VERSION     DAOMXO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10303_length_4517_cov_5.092784"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1267
                     /locus_tag="PLH57_03255"
     CDS             125..1267
                     /locus_tag="PLH57_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRK01656.1"
                     /translation="MASFKDLAFTSAEFARLAQISKNQVDEFEKEGVLTTIRKKVGNV
                     DRKMVPLESAQSFFSKLREMNVPSVAAATETPNEAGDPSLAAGSDSSEVAMNASTGRH
                     GDQKAPIGMFLPKSVQHKVQMFYNVKGGTGKSTLTAQFIMQAALMGYRVLGIDLDGQG
                     HLSLNLDVLNAHELPTIYDVLINDLPMKDSIINVAPGLDLVPANLGLCTIEIPLNQKP
                     RREYRLGEAIRSVYDQYDLIVADTNPAASILNSSMLVAADQVNVVCATNPLSFHGMSL
                     VMATIEEMEREFRKELAIKIIPNMYDNREVISQEVLGELKSQFGDYCARSVISRSVDL
                     NEATKQRTSVWGVNSRGIGAQDIGALANELIAQGQAELGSASLGNA"
     gene            1350..2243
                     /locus_tag="PLH57_03260"
     CDS             1350..2243
                     /locus_tag="PLH57_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRK01657.1"
                     /translation="MDEKKVKTREGSGYLRQLYSTARNTAGTNSSNFKSLTTFLVNFI
                     PVDKVDASELQVRVHFDDAEIDALANSIKVHGVLQPILVVQNGDRYKVIAGERRLRAS
                     KKAGIERIPARVINSDDKGVHEIALRENLDRVDLHPIEEGEGYLSLLEAQAYTSHESI
                     AKAFLRPKSRITECIGYTKLPDATKKEIMRRNIKARSFMRKLMLVPPEQHMQLIEDAV
                     KDLESEPTLVAASAVLDSPEAEAKPKPKREVKSFEYSVSDKAISVPGFRWKVGEGTDR
                     LLDYAAQVEKLLADVKKVIEI"
     gene            complement(2370..3017)
                     /gene="pdxH"
                     /locus_tag="PLH57_03265"
     CDS             complement(2370..3017)
                     /gene="pdxH"
                     /locus_tag="PLH57_03265"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRK01658.1"
                     /translation="MTKKEHSVFRWRPALQYKSLYSARGAQDPIQLFEKLFIEARKAL
                     PMPEAMTLATADKKGTPSARVVLLKGIDQGGFCFFTNYLSKKAKDLAQNPRAALMFFW
                     EPLEIQIRIDGKVRKTNAKYSDDYWKSRPRASQIGALASPQSTEITSFEKLAEAAQTI
                     ETAFEGLEIPRPKRWGGYTVTPTRIEFWFGKPNRLHERVAFEKSKSRWKSRILGP"
     gene            complement(3043..3957)
                     /locus_tag="PLH57_03270"
     CDS             complement(3043..3957)
                     /locus_tag="PLH57_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01659.1"
                     /translation="MNLLFFTLLPFLFLFINTVTISPSAASACCASSTAAPSLMTEDS
                     KAQTTLSSSYSDTIGHSSDDGSSVFYNEERSDTVLRFRLSQTIALDDRWQISASTDLL
                     RTEKKLSGARESTWAWGDTSLGASYEILPEYTYYPIRPRVFLSSATFLPTGRAADARS
                     DRLGTNVSSNGAWGFAVSGHALKTWSRWDTSLTSQHVRYLSQTRTVGRNQVRFNPGAL
                     TTVDLAGGFHSAVFGLPSRFGLSVGPEWQASAAPRLVWNVGGSVSVTPHQDWAASLNY
                     NDQTLLGPTYNATLSRQATLRLTKRLGG"
     gene            complement(3954..4376)
                     /locus_tag="PLH57_03275"
     CDS             complement(3954..4376)
                     /locus_tag="PLH57_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01660.1"
                     /translation="MRGLNYFLYFFPAGFAFLLSACGVSPLFQHEDEKTPPPAHRNEV
                     EPQHIVKIANGTAQIAWKDGVSVGESSFTVTSDQPITQFDSWADMPDMGHGTSVFNFE
                     RISDREYFVSEVWFTMPGRWVLSVEINQQTFEFELDVQ"
BASE COUNT         1183 a         1033 c         1183 g         1118 t
ORIGIN      
        1 aaaaaaagaa ggtcgcttca taaatttctg cccttttttg gaagcgcgtt ttatgatctg
       61 tgaatctgga gtttttttcc gatcgtacgt caagatacat tttcaaccag ggagggtgtg
      121 caaaatggct tcgttcaaag atctcgcatt tacatctgcc gagttcgcaa gacttgcgca
      181 gatatcaaaa aaccaagtag atgaattcga aaaagaaggg gtgcttacca ccatccgaaa
      241 aaaggtcggc aatgtagatc gcaagatggt tccgcttgaa tctgctcaat cgtttttttc
      301 gaagctcaga gaaatgaatg ttccttcggt cgccgcggcg accgagacac cgaatgaagc
      361 gggcgaccca agtttagctg ccggctcgga ttcttctgag gttgccatga acgcttccac
      421 cggtcgccac ggcgaccaaa aagcgccaat tggaatgttt ttgccaaaaa gcgtgcagca
      481 caaggtgcag atgttctaca acgtgaaggg tggtacgggg aaatcgacct taactgcgca
      541 atttattatg caagcggcgc tcatgggcta tcgagtttta ggaattgatc ttgatggaca
      601 ggggcatttg agtttgaacc tcgatgtatt aaatgctcac gagcttccga cgatctacga
      661 cgtgctgatt aacgatctac cgatgaaaga ttcgatcata aacgtggcgc cgggccttga
      721 tttagtgccg gcaaacctcg gtttgtgcac gatcgagatc ccacttaatc aaaaaccacg
      781 tcgcgagtat cgtttgggcg aggcgattcg ctcggtatac gaccaatatg acctaattgt
      841 ggccgatact aatccagcag cctcgattct taatagttct atgctcgtag cggcagacca
      901 ggtgaatgta gtttgtgcca caaatccact ttctttccat ggaatgtcgc tcgttatggc
      961 gactattgaa gaaatggaac gtgaattccg caaagagctc gccataaaga tcattccaaa
     1021 catgtacgac aaccgcgagg ttattagtca ggaagttttg ggtgaattga agtctcaatt
     1081 cggagattac tgtgctcgtt ccgttattag ccgtagtgtg gatttaaacg aagccactaa
     1141 acagcgtaca agtgtttggg gcgtaaattc acgcgggatc ggtgcgcagg atatcggagc
     1201 gttggccaac gaactaatcg ctcaaggaca agcagaactt ggttctgctt cgctagggaa
     1261 cgcttaaatt agtttgaagc gtcgcagcag atcgttgaaa gcgaatcaca aagcgacaaa
     1321 cgttcaaaaa atcacggagg atttgaatta tggatgagaa gaaagtcaaa accagagaag
     1381 gtagcggtta cttaaggcag ttgtattcca ctgcgcgcaa tacagcggga accaattcat
     1441 caaactttaa aagtcttacg acgtttttgg tgaactttat tccagtagat aaggtcgatg
     1501 cttcggagct ccaagtcaga gttcacttcg acgacgctga aatcgatgct ttggcaaatt
     1561 cgattaaggt gcacggtgta ttgcaaccga ttctcgtggt tcagaacggc gaccgttata
     1621 aggttatcgc gggtgagcgc cgactaagag cctcgaaaaa agcagggatc gaacgcattc
     1681 ctgcgcgggt cattaacagt gacgacaaag gtgttcacga aattgcactg cgtgaaaact
     1741 tagatcgcgt tgacttgcat ccgattgaag aaggcgaagg gtatctgtct ttgcttgaag
     1801 ctcaggcgta caccagtcat gaaagcattg cgaaggcgtt tctaaggccg aagtctcgta
     1861 ttacggaatg cattggttac acgaagttgc ccgatgccac caaaaaagaa attatgcgtc
     1921 gcaacataaa ggcgcgctcc tttatgagaa agctcatgtt agtgccgccc gagcaacaca
     1981 tgcagttgat cgaagacgcg gtgaaggatt tagaatccga acccacttta gtcgccgctt
     2041 cggcagtact cgatagccca gaggcagaag caaaaccaaa gcctaaacga gaagtaaagt
     2101 ctttcgagta tagcgtttcc gacaaggcca tcagtgttcc gggttttcgt tggaaggttg
     2161 gcgaaggtac agaccgtcta ctcgattatg cggcacaggt tgaaaaactt ctggccgatg
     2221 taaaaaaagt catcgagatt taatcacacc caaagctcaa agagattacg tcaaagtctc
     2281 tttgagcttt ttttccctat ccacgaatca cttgcgcgca atcaagttca aactctttct
     2341 gaacagatag accgttttag gctggtgaac tacggaccta ggatcctgct tttccaacgc
     2401 gacttacttt tttcgaaagc tactcgttcg tgcaggcggt tgggtttgcc gaaccaaaat
     2461 tcgattcgag taggcgtgac cgtatagccg ccccaacgct tgggccgtgg tatttcgaga
     2521 ccttcaaatg cggtttcgat ggtttgtgcc gcttcggcca gtttttcgaa cgacgtgatt
     2581 tcggtgcttt ggggcgacgc taatgcgcca atctggctcg cgcgtgggcg ggacttccag
     2641 tagtcgtcgg aatacttggc gttcgttttg cgtacttttc cgtcgattcg aatttgaatt
     2701 tcgagcggtt cccagaaaaa catcaacgcc gctcgcgggt tttgggccaa gtctttggct
     2761 tttttggaga ggtaattcgt gaaaaagcaa aagccacctt ggtcgatgcc tttaagcaaa
     2821 acgacgcggg cgcttggcgt tccttttttg tcggcagttg ccaaggtcat ggcttcgggc
     2881 atcggaaggg ccttgcgcgc ttcgatgaaa agtttttcaa aaagctgaat cgggtcctgg
     2941 gctccgcgtg ctgaataaag cgatttgtat tgaagtgcgg gacgccaacg aaatacggag
     3001 tgttcttttt ttgtcacggg cgcaccatac tcccgatcag ccctaaccgc caagtctttt
     3061 ggtcaatcgc agtgttgcct gccgactaag ggtcgcgttg taagtgggtc ccaggagtgt
     3121 ttgatcatta tagtttagtg atgccgccca gtcttggtgg ggggtaaccg aaactgagcc
     3181 gcccacgttc catactaaac gaggagcggc gctggcttgc cattcaggtc ccaccgacaa
     3241 gccaaatctg gaaggtaggc cgaagactgc tgagtgaaag ccgccggcca ggtcgaccgt
     3301 tgtgagtgcc cccggattga aacgaacttg gtttcgccca accgttcgag tttgcgaaag
     3361 gtatcttacg tgttggctgg tgaggcttgt gtcccagcga gaccaagttt ttagggcatg
     3421 cccactgact gcaaatcccc acgcgccatt gctcgacaca ttggtaccca agcgatcaga
     3481 gcgagcgtct gctgcgcgcc ctgtgggtaa gaaagtagca gagcttagaa acactcgagg
     3541 tcgaatggga tagtaggtat attcgggcaa aatttcgtag ctggcaccga gcgaggtgtc
     3601 cccccaagcc caggtggatt cgcgcgcacc actcaacttt ttttcagttc gtaacaaatc
     3661 ggtgctggcg ctgatttgcc accggtcatc aagggcgatc gtctgggaaa ggcgaaaccg
     3721 cagtacggtg tctgaacgtt cttcattata aaagacgctc gaaccgtcgt ccgatgaatg
     3781 accaatggta tcggagtaac tagaagaaag agtagtttga gctttgctat cttcggtcat
     3841 taaagacgga gcggcggtgg agctagcgca acaggcggag gcagcggacg gactgatagt
     3901 gacggtgttg ataaataaga acaaaaaagg gagcagcgta aaaaaaagga gattcactgc
     3961 acgtccaact caaactcgaa agtctgttga ttgatttcca cggataaaac ccatcgacca
     4021 ggcatagtga accaaacttc acttacgaaa tattcccggt cggatattcg ctcgaaatta
     4081 aacacggagg taccatgacc catgtctggc atatctgccc aagagtcgaa ttgggtgatg
     4141 ggttgatcac tcgttactgt gaatgaagat tcgccgacag atacaccgtc tttccaggcg
     4201 atttgggcgg ttccatttgc gatctttact atatgttggg gctcgacttc gttgcggtga
     4261 gccggcggcg gagttttttc gtcttcgtgc tgaaacaacg gtgatacacc gcacgccgaa
     4321 agcaaaaagg cgaaacctgc gggaaaaaaa tatagaaaat aattaaggcc gcgcaatgaa
     4381 gcaccccaaa gttttaacga gtgcgggttc gggtagttgc ccgttcgcaa tttgattaaa
     4441 cgcggtttcc aaaatttcct tttccgcaat ttttgcatca cgcgcatcgt cgacaccgcc
     4501 cgaaaaaata acgtctc
//
LOCUS       DAOMXO010000106         4509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10336_length_4509_cov_3.709026, whole genome shotgun sequence.
ACCESSION   DAOMXO010000106 DAOMXO010000000
VERSION     DAOMXO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10336_length_4509_cov_3.709026"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..767)
                     /locus_tag="PLH57_03280"
     CDS             complement(150..767)
                     /locus_tag="PLH57_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010521129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRK01661.1"
                     /translation="MKLASVFLAFLALTSLSASAAGEPVKMEINTSESKVEWFASKVK
                     GKHNGTIEVESGEFELDGKGMIKSGKIEIDMDSIKVSDIEDAEDNAKLAGHLKSDDFF
                     NAEKWPTATFVIKTALPLGKRNSDGWQKYTLKGDLTIRDVTKPFTAHANVKIAGEAAE
                     ATAELNIDRTQWNVKYGSGKFFKGLADRMIHDVFKLKIALKAEKD"
     gene            complement(818..2494)
                     /locus_tag="PLH57_03285"
     CDS             complement(818..2494)
                     /locus_tag="PLH57_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRK01662.1"
                     /translation="MAESNDSSSTAETQKSFGEPGLSTVLAREVARRRTFAIISHPDA
                     GKTTLTEKLLLYGGAIHLAGAVKSKTKTKATTSDWMEIEKQRGISVTSSVLQFEYKGY
                     CINLLDTPGHKDFSEDTYRTLTAADAAVMLIDAAKGVEAQTRKLFEVCRMRGIPIFTF
                     VNKLDRPGRDPIELLDEIEKVLGIRSCPMTWPIGTGDRFKGVYDREKKEILFFQTEGQ
                     ANVSGRLKPKKVGQLQELGSEFDEETLQSVELVELAGDPFDHERVLRGELTPVFFGSA
                     MNNFGVEHFLERFIELAPPPVGRKTSDSHLAEKKLIEPTSTKFSAFFFKIQANMDPSH
                     RDRVAFMRVCSGKFTRGMTVKHVRLGKDVKLAMPVNFFGQERTVIEEAWPGDIVGVLD
                     TSGDLRIGDTLAQGGGFEFEGVPQFSPEHFASVTILDPMKRKQLQKGLDQLSEEGVIQ
                     VYRQRDLSDKDPVLGAVGALQFEVLKHRLLSEYSVNVNIEKLSYTCARWVEGENFDVD
                     GCERREGNRCLVDRDANLVVLFKSDWALRWAEDNHKGFRFLKTPLARRRN"
     gene            complement(2507..3244)
                     /locus_tag="PLH57_03290"
     CDS             complement(2507..3244)
                     /locus_tag="PLH57_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRK01663.1"
                     /translation="MGAQWKQAGREQNANKKGIIIGKLAKEISVAAKLGDPNPENNPR
                     LRMAVEAARKHSVTRDTIERAIKKGAGLLDEQVNYELVTYEGFCPHQVPVIVECLTEN
                     RNRTAAEIRVLFRKGQLGSSGSVAWMFDRLGVIEASTAEGGLDIEGVAIEAGAQNVEP
                     IAGDELKQMNPDHKTGARFICEPQDLDSVSKAVTALGWKVVSAELSYLAKNNVEISDE
                     QKKEVTAFLNDIDDFDDVHRVYVGLKS"
     gene            complement(3281..3772)
                     /locus_tag="PLH57_03295"
     CDS             complement(3281..3772)
                     /locus_tag="PLH57_03295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRK01664.1"
                     /translation="MQKSLQESFAPTSICFGCGPANQKGLRIRTLPVSGVADSTNGKS
                     YLTEAEWVPEKHHEAFPGMLNGGIIGSLLDCHSNWTAAWALMQKQSLTQPPCTVTADY
                     AIVLKRPTPTSGPVRLRAWVKTLGEDRATIEAELEAGGKICATCTGTFVAVKPGHPAY
                     HRW"
     gene            3872..>4509
                     /locus_tag="PLH57_03300"
     CDS             3872..>4509
                     /locus_tag="PLH57_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01665.1"
                     /translation="MTTVGLSLRKWGLAIAVAGAILLFSIGEPYFSAHYFRQAQTLET
                     ASFFSQLGFDFFNPRVGYRGKPGILALEAPFYQAAIHFLKGATGLSPESIGRFISGLF
                     AIAFALIATSFLISVLGKKNAATTNNTILSFEKVFPLILSTPLLFGMGQWVTIENLHL
                     ASGTFSLFSMIQFFRTETKREQISWIIAYVVAATFSLLIKPTTMFALASLFV"
BASE COUNT         1083 a         1177 c          986 g         1263 t
ORIGIN      
        1 catttcttcg atattacgaa gcaaggcggg gttgccatca aaacgaagat cacgacgaaa
       61 ccagtgaaga cctatagatt tcataaaaaa aggggctcct tagtattatt aggaacccct
      121 tccttacatt agtttttaaa attcgcgatt tagtcttttt cagccttcaa tgcaattttt
      181 agtttaaaca catcgtgaat cattctatcg gcgagacctt taaaaaattt accagatcca
      241 tattttacat tccactgagt gcggtcgatg tttaactccg cagtggcttc ggccgcttca
      301 ccggcgattt tgacgttggc gtgggcggtg aacggtttgg ttacgtcgcg aatggttagg
      361 tcacctttga gagtgtattt ttgccaccca tcgctattgc gcttgcccag tgggagcgcc
      421 gttttaatca cgaacgtagc cgttggccat ttttccgcgt taaagaaatc gtcggacttt
      481 aagtgccctg ccagtttcgc gttgtcctcc gcgtcttcga tgtcggagac tttgatcgaa
      541 tccatatcga tttcgatttt tccagacttg atcattccct tgccatcaag ttcgaattca
      601 cccgactcta cttcgatcgt tccgttgtgt ttacccttaa ctttgctagc aaaccattca
      661 accttacttt ccgaagtgtt gatttccatt ttaaccggct caccggcggc cgaggccgaa
      721 agtgaggtga gcgccagaaa tgctaaaaaa actgaagcca atttcatagg atgttccttt
      781 cagtacgaag aataaaagtg aagctataag gataaattca gtttcgccgt ctcgcaagcg
      841 gtgtctttag aaagcgaaat cctttgtgat tatcttccgc ccaacgcagc gcccaatcgc
      901 ttttaaaaag aaccactaga ttagcgtcgc ggtccactaa acaacggtta ccttctcgcc
      961 gttcgcagcc gtcgacgtcg aagttttcgc cctcgaccca gcgcgcacag gtgtaggaca
     1021 atttttcaat attcacgttg accgaatatt ctgacagtaa cctatgtttg agaacttcga
     1081 actgcagcgc acccactgcg cccaagacgg ggtctttatc actaagatcg cgctgccggt
     1141 aaacttggat cacgccttct tctgaaagct gatctaatcc cttttgaagt tgcttgcgtt
     1201 tcattggatc aagaatcgta acggatgcaa aatgttctgg cgaaaactgc ggcacacctt
     1261 cgaactcaaa gccccctccc tgcgctaggg tatctccaat acgcagatcg cccgaggtgt
     1321 ccaacacacc cacgatatcg cctggccacg cttcctcaat taccgtgcgt tcctgaccaa
     1381 aaaaattcac cggcatcgca agtttgacgt ctttgcctaa ccgcacgtgt tttaccgtca
     1441 tgcctcgggt gaacttaccg gaacaaaccc gcataaaggc gacacgatcg cggtgcgaag
     1501 ggtccatatt cgcctgaatt ttaaaaaaga aggccgaaaa tttggtcgag gttggttcta
     1561 tgagtttttt ctcagccaaa tgagaatcag aagtctttcg accgacggga gggggcgcta
     1621 gctcgatgaa acgctctaga aaatgttcca cgccaaaatt gttcatggca ctaccaaaaa
     1681 acaccggagt taactcgccg cgcaataccc gttcatgatc gaacggatcc ccagccaatt
     1741 cgactaattc aacgctctga agagtttctt cgtcaaactc gctccccagc tcttgaagtt
     1801 ggcccacttt ctttggcttc aagcgaccgc taacgttcgc ttgcccctcg gtttgaaaaa
     1861 acaggatctc ttttttctcg cgatcgtaca cgcctttaaa tcgatctccg gttccaatcg
     1921 gccaggtcat gggacaggaa cgaattccta aaactttttc gatctcgtcg agcagttcga
     1981 tagggtctcg tcccggacga tcaagcttgt tgacgaacgt aaaaatagga atgccacgca
     2041 tgcggcatac ttcgaagagt tttcgcgttt gagcctcaac gccttttgca gcatcgatca
     2101 acatcactgc ggcatcggca gcagtgaggg tacggtaggt atcttcggag aagtctttat
     2161 gccccggagt gtcgagcaaa ttgatacagt agcccttgta ttcaaattgc agcacgcttg
     2221 aagttaccga tataccgcgc tgcttttcga tttccatcca gtctgaagtc gtggcttttg
     2281 ttttcgtttt cgattttaca gcgcctgcta agtgaatggc tccgccgtaa agcaaaagtt
     2341 tttcggtgag tgtggtttta cctgcatcgg ggtgagaaat gatcgcaaaa gtacgtcgac
     2401 gcgcaacttc acgcgctagc accgttgaaa gcccgggttc accgaacgat ttttgtgttt
     2461 cagcagtact cgaggagtcg tttgattccg ccataaacct ctgaatttaa gactttaggc
     2521 caacgtaaac acgatgaacg tcatcgaaat cgtctatatc gttcaaaaat gcggtgactt
     2581 cttttttctg ctcatcggat atttcaacgt tattttttgc caaataacta agttccgcag
     2641 aaaccacttt ccagcccaat gctgttaccg ctttcgatac agaatctaaa tcttggggtt
     2701 cacaaataaa ccgggcaccg gttttgtggt ccggattcat ttgtttaagc tcgtcgccag
     2761 cgatcggctc aacattctgc gcccccgctt caatggctac tccctcaata tcaagtccac
     2821 cttccgcggt ggatgcttca attacaccga ggcgatcgaa catccacgcc acactaccgg
     2881 aactacccag ttgccctttg cgaaataata cccgaatttc ggcggcggta cggtttcgat
     2941 tttcggttaa acattcgacg atcactggaa cttgatgagg acaaaacccc tcgtaggtca
     3001 caagctcata gttgacttgc tcgtctaaga gccctgcccc ttttttgatt gcccgttcga
     3061 ttgtgtcgcg cgtaaccgaa tgtttgcgcg ccgcttcgac tgccattcga agcctggggt
     3121 tgttttccgg gttcggatca cccaatttgg cggctactga aatttcctta gcaagcttgc
     3181 caatgataat gcccttttta ttggcgttct gttcacggcc ggcctgtttc cactgtgctc
     3241 ccatgagtcc tctcttcgct actctttctt ctttaacctg ttaccaccga tgataagccg
     3301 gatgccccgg cttcactgcc acgaacgtgc cagtgcacgt cgcacaaatt tttccacctg
     3361 cctcaagttc agcttcgatc gtagcgcggt cttctccgag cgttttgacc caagctcgta
     3421 gccgtaccgg accactggtc ggagtaggtc tcttcaacac aatggcgtaa tctgctgtaa
     3481 cggtgcaggg cggctgagtt aagctttgct tttgcattaa cgcccaagca gcagtccaat
     3541 tagagtggca atcgagcaac gagccaataa ttccaccatt gagcatgcct ggaaacgctt
     3601 catggtgttt ctcgggtacc cactcggctt ccgtaaggta actcttccca ttggttgaat
     3661 ccgcaacccc agaaaccggc aaggttcgga tgcgcagccc cttctggttc gcggggccgc
     3721 atccaaagca aatgctcgtt ggtgcaaagg actcttgcag gcttttttgc ataaaaacct
     3781 tctaacgaac tcaggttggc aattcaagtg cgagtacggt atttcaactg ctcgcttgcc
     3841 atcccaggaa agtgattcta acgataaaaa aatgacaacc gtcggtcttt ctctaagaaa
     3901 atggggtcta gcaatcgcgg tcgctggcgc catactgcta ttctcgatag gtgagcctta
     3961 ctttagtgcc cattactttc gccaagccca aacacttgaa actgcctcat ttttttcaca
     4021 attgggcttc gactttttta acccacgagt ggggtatcgc ggaaaacccg gaattctggc
     4081 actggaggcc cctttttacc aagctgcaat tcacttttta aaaggcgcaa ccgggttatc
     4141 gccagaatcc atcggacgct ttattagcgg attgttcgca atcgcttttg ctttgatcgc
     4201 tactagtttt ttaatctctg ttttaggaaa aaagaacgcc gccaccacga acaacaccat
     4261 tttatcgttc gaaaaagttt ttccgttaat tctttcaaca ccacttctat ttggaatggg
     4321 ccagtgggtc acgatcgaaa acctacatct ggcgagtgga actttttccc tattttctat
     4381 gattcaattt tttcggactg agaccaagag ggaacagatt tcttggatta tcgcctatgt
     4441 tgtcgcagct actttctcgc tacttattaa accaaccacg atgtttgcgt tggccagtct
     4501 ctttgtata
//
LOCUS       DAOMXO010000107         4467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10469_length_4467_cov_4.353808, whole genome shotgun sequence.
ACCESSION   DAOMXO010000107 DAOMXO010000000
VERSION     DAOMXO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10469_length_4467_cov_4.353808"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLH57_03305"
     CDS             complement(<1..409)
                     /locus_tag="PLH57_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK01666.1"
                     /translation="MKFHSSFFTSVLLAAGTLVLAPGCNKSTQTGSTATDPANEIVIG
                     EVGSLTGNEATFGTSTRDGIRLAIEQINQSGGIQNKKLRVVTMDNQGKPEEAATAVTK
                     LITQDRVIAILGEVASSRSLAMAPIAQTNKVPMV"
     gene            468..1373
                     /locus_tag="PLH57_03310"
     CDS             468..1373
                     /locus_tag="PLH57_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRK01667.1"
                     /translation="MSIGFDRAKESFLVELAAQGKASNTIKNYRCDLGELREYCSLTQ
                     CDFLSLGVAELENYVLYLRKKGLKTNSCRRKLMTARKFIRFLSGKVDVATIGIEKHIP
                     PEKVEKPPRLVDLEVIQEVLELQPETDIGLRNRALIHVLLDTGMLVTEALALRTSDLH
                     GFSLKVGGKRARMVMISKETAHSIALLKDKLHQHFKGKTFLFHGYSKAGPYPERMTAR
                     GVELLFKAWGRQLGQKNLRPRVLRHMYIVSELQKGRTEGQIMGMVGLKTPYSFRVYRQ
                     IQDSNHIRERKASGVSGQLENTGKK"
     gene            complement(1292..2560)
                     /locus_tag="PLH57_03315"
     CDS             complement(1292..2560)
                     /locus_tag="PLH57_03315"
                     /inference="COORDINATES: protein motif:HMM:NF027518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01668.1"
                     /translation="MTTGHLLQNRMLHYVRINWFRFRLLWFVPIVILFGVVLILVESS
                     QRPPTYYFDENHYVPAGKMLVYQGEDLNPEHPPLGKWIIGWSAIAFGALTKSGLGFRL
                     PSLLMGLLALGCLGLLLKRLGFSSHQALCAVTLGALNGLWFVHAKIATLDIYYTALGL
                     GALALAAHRHSIVSIAAFALACSTKWSALMYFPILLLMEYREQRLWGTRAALKLVAQR
                     GFALILGAFAVRTFLDVVLYRRFSLGWVVREFNLNVDIRNSIAHPYETSFWQWPFMLR
                     PIWFHHDTISADAVTGVWAGGNPFIWALALLAFIGLLVTLKRGRLRGVFFTRIMLLVG
                     FTAPLWFWGFAPAHAQYFYYFLPGSLWAGPLIVFGITRIVRHERAAQYVLTLTLLASA
                     LVFFYFLPVFSNWPLTPEALRSRMWFESWI"
     gene            2609..3208
                     /locus_tag="PLH57_03320"
     CDS             2609..3208
                     /locus_tag="PLH57_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01669.1"
                     /translation="MLKPNGWTLFFIAALWAISIQPILAQEAGSFLLTCEGEALVAGQ
                     KKPVKLAISKIGASSKVTTKEDDYSVLVDLAAFSTPPDTSNPAASAEHNIKENLQSIE
                     FSTKGSKDKWAVDLRLVPEFDIETGALARMRIHSGLVKSSASKTALEKRTNANLGSLK
                     NTYETFVGVSLEAVPTNFTSIELYPHKGLAWVGITCEVM"
     gene            complement(3201..4298)
                     /locus_tag="PLH57_03325"
     CDS             complement(3201..4298)
                     /locus_tag="PLH57_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRK01670.1"
                     /translation="MISKIYWRLLAAVHGWLEKKLKPISAIQAPPIIIPEPAPLPVPI
                     EPQQSVGEQELWQTLWAFLDSFLKTQNSPHTRRAYERDLEDFLCFIDSNRTTISVHSL
                     VRYREELRTKNSSQTGQPLSHVSINRKIATIKSFLNWLVLNGVLPNNPAKAVKSFKAG
                     RDSPTRDIPDDRVRAMLDLPARHTVAGRMHHAMLMVLFHLGLRRSELVDLRTSDLFER
                     EGMRVLRVRGKGDKERVLPVPEPVQNSLDQYLLIARKTLDVDQPLFTPVKNNVTKTKE
                     KNLHPNAVAYVVKHYAKLAGIHYRVSPHSARATAVSNALDHHAPHRSVQHMAGWSSPL
                     MVTRYDKRKEELKNSAVRFVQYSSDPDEELT"
BASE COUNT         1225 a         1051 c         1113 g         1078 t
ORIGIN      
        1 ttaccattgg tactttgttg gtttgggcaa ttggcgccat tgcaagacta cgagaactcg
       61 ccacctctcc aagtatggcg attacgcggt cttgagtaat taattttgtg acagcagtgg
      121 ctgcttcttc gggttttccc tgattgtcca tcgtaacgac gcgcagtttt ttattttgaa
      181 taccgcccga ctggttgatc tgttcaatag caagtcgaat tccgtcgcga gtgctggttc
      241 cgaaggtggc ttcgttccca gtgagacttc cgacttcacc gatgacgatt tcatttgcgg
      301 gatctgtcgc ggttgagccc gtttgcgtag atttattaca tcccggcgcg agtacgagcg
      361 tgcccgccgc aagtagcact gaagtgaaaa aactcgaatg aaatttcatg attcccccca
      421 aaccagtgtt gacgaacact cagaagtttt aaacaatcgg tctagacatg tctatagggt
      481 tcgatcgcgc aaaagaaagt tttttagttg aactcgccgc gcaaggcaaa gcgtcgaaca
      541 cgataaaaaa ttatcgttgc gacctgggag aattgcgtga gtattgttca cttacacagt
      601 gtgatttctt atcgctgggc gtcgcagagt tagaaaatta tgttctttat ctgcgtaaaa
      661 aaggattgaa gaccaattct tgtcgccgaa aactaatgac ggcacggaag tttattcggt
      721 tcttgtctgg aaaagtggac gtcgcaacga tcggaattga aaaacacatt cctccggaga
      781 aggttgaaaa gccgccacga cttgtggatc tcgaggtgat tcaagaagta ctcgagctgc
      841 agccggaaac cgatattgga ttgcgcaacc gggctctaat tcatgtctta ttggacacgg
      901 gaatgctggt caccgaggcc ttggcgctac gaacctccga cttgcacggg ttttcgttga
      961 aggtcggcgg aaaacgcgca cgaatggtga tgatttccaa agaaaccgca cattcaatag
     1021 ccttgttgaa agacaaactg caccaacact ttaaaggaaa aacgtttctc tttcacgggt
     1081 attcaaaggc cgggccctat cccgaacgaa tgacggcacg cggagtggaa ctgttgttca
     1141 aagcctgggg gcggcaattg ggccagaaaa accttaggcc aagagtgctt cgccacatgt
     1201 acattgtatc ggaacttcaa aagggtcgta ctgaagggca gatcatgggc atggttggtt
     1261 taaaaacacc ttattcattt cgggtttatc gtcagatcca agattcaaac cacatccgtg
     1321 aacgcaaggc ttcgggagtg agtggccagt tagaaaatac gggtaagaaa taaaaaaaga
     1381 ctagagctga agcgagtaag gtgagtgtca gcacgtattg agcggcgcgt tcgtggcgca
     1441 cgattcgggt gattccaaac acaatgagcg gcccagccca aagcgagccc ggcaaaaagt
     1501 agtaaaaata ctgggcatgc gcgggggcaa atccccaaaa ccacagcggt gcagtgaaac
     1561 ctacaagcag catgatccga gtaaaaaaaa caccgcgcaa tcgtccgcgc tttaaagtga
     1621 caagtaggcc aataaaagca agcaaagcga gcgcccatat gaacgggttg ccaccagccc
     1681 acaccccggt cacggcatcg gcggaaatcg tgtcgtgatg aaaccatata ggtcgcagca
     1741 taaaaggcca ttgccaaaag ctagtctcgt aggggtgtgc gatcgagttt cgtatatcca
     1801 catttaaatt aaattctcgc accacccaac ctagcgaaaa ccgccgatac aaaaccacat
     1861 ccaagaaggt tcgaaccgcg aacgccccta aaattaaagc gaaccctcgt tgcgcaacta
     1921 gttttaacgc cgctcgcgtg ccccagagtc gctgctctcg gtattccatt aataacagta
     1981 tcggaaaata catgagcgct gaccatttcg tagaacacgc gagcgcaaag gccgcgatgc
     2041 tcacaatcga atggcggtga gcggctaagg cgagcgctcc tagccccaag gcggtgtaat
     2101 aaatatcaag tgttgcgatt ttcgcgtgca caaaccacaa accattgagc gcgccaagtg
     2161 ttacggcaca tagcgcttgg tggctggaaa aacctaaccg tttaagcaac agaccgagac
     2221 agccaagcgc caataggccc attaaaagac tcggcaaccg aaatcccaaa ccacttttcg
     2281 ttaaggcacc aaacgcaatg gccgaccagc ctattatcca ttttccgagc ggcgggtgtt
     2341 cggggttcaa atcttcgcct tggtaaacca gcatttttcc cgcgggcacg taatgatttt
     2401 catcaaaata gtaggtgggt ggacgttgcg agctttcaac tagaattagg acaactccga
     2461 aaagtatgac tatcggcaca aaccacagga gtcgaaatcg aaaccaattt attcttacgt
     2521 agtgcagcat gcgattttgt aaaaggtgtc cagtggtcat tgtacaactg atgttcctct
     2581 gatacctaat gattttggga ggcacggaat gctgaaaccg aatggatgga cccttttctt
     2641 tatcgcagct ctttgggcga tttcaattca accgattctc gcgcaagagg caggttcttt
     2701 tcttttaacc tgcgaaggcg aagcactcgt ggcaggtcaa aaaaaaccag tgaaactcgc
     2761 aatttcaaaa attggcgcat cttcgaaggt aaccacaaaa gaagacgact atagtgtact
     2821 ggtggacctt gcggcttttt cgaccccacc ggacacttcg aatccggcgg cgtcggccga
     2881 acacaacata aaagaaaatc ttcagtcgat tgagttctcc accaaaggta gcaaagacaa
     2941 gtgggccgtc gacctgcgat tagttcctga atttgatatt gaaactgggg cgctcgcgcg
     3001 catgaggatt cacagtggat tggtgaagtc gtcagcttcg aaaaccgctc tggaaaaacg
     3061 tacgaatgcc aacttgggat cgttgaaaaa cacctacgag accttcgtag gtgtatcgct
     3121 agaagccgtt cccacgaact ttacttctat cgagctctac ccccataaag gtttggcctg
     3181 ggtcggaatt acctgcgaag tcatgtaagt tcttcatctg gatcagatga atattgaaca
     3241 aaccgcaccg cggaattttt aagttcttct tttcgtttat cgtagcgcgt aaccattaga
     3301 ggtgaactcc aacccgccat gtgttgtact gaacgatggg gcgcgtggtg gtcaagggcg
     3361 ttcgaaaccg cggtagcccg cgcggagtgc ggcgacacgc ggtaatgaat gccggcaagt
     3421 ttggcgtaat gtttaactac gtaggcgacc gcattggggt gtagattctt ctcttttgtt
     3481 ttggtgacat tgtttttcac aggcgtaaag agtggctgat ctacgtctag agtttttcga
     3541 gcgattaaaa gatattgatc taagctattt tgaactggct ctggtaccgg taacacacgt
     3601 tctttgtctc ctttacctcg aacccttagt acccgcattc cttcgcgttc aaaaagatca
     3661 gatgttcgca aatcaactaa ctccgaacgg cgcaggccaa gatgaaaaag caccatcaac
     3721 atggcgtgat gcatacgacc ggctactgtg tgacgggccg gcaagtctaa catagccctc
     3781 acgcggtcgt cgggaatatc gcgagtgggc gagtcgcgac ccgctttaaa agacttaacg
     3841 gcctttgccg gattgttcgg caatacgccg ttgagaacga gccagttcaa gaaactctta
     3901 atggtcgcaa tcttacgatt gatcgacacg tgagagagcg gctgccctgt ttgagaacta
     3961 tttttggttc gcaactcttc gcgatatcgc acgagggaat gcacgctgat cgttgttcga
     4021 ttggagtcta tgaaacaaag aaaatcctct aaatcacgtt cgtaggcgcg tcgcgtatgc
     4081 gggctgttct gcgtttttag aaacgaatcc aagaatgccc atagggtttg ccaaagctct
     4141 tgctccccga cggattgctg cggctcgatt ggtaccggca aaggtgcggg ttccggaatg
     4201 ataatgggag gggcctgaat tgctgaaata ggtttgagtt ttttttctaa ccaaccatgc
     4261 actgcggcga gaagtcgcca gtatattttc gaaatcaccg tacccatgac tcaagattat
     4321 gaaggcgaat tccgaattag aaaagactaa accctttatt ggcggataat tattattgat
     4381 aattaaactt atcaccaata aagaatctag gccaatgggc ctggccccaa atttggatgc
     4441 gaatcaaata tgaacagttg gcttcat
//
LOCUS       DAOMXO010000108         4447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10530_length_4447_cov_4.679417, whole genome shotgun sequence.
ACCESSION   DAOMXO010000108 DAOMXO010000000
VERSION     DAOMXO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10530_length_4447_cov_4.679417"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PLH57_03330"
     CDS             <1..1120
                     /locus_tag="PLH57_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01671.1"
                     /translation="FETIRELKKQGVSAADRRRAVLPKLSATTVRARFDDAKKSGKKE
                     IYLPELVPDVRWALASLKERHDAAIRKSPFTPEQINHLEEAAEFYRLRFSSILDDPRG
                     VPMRDWLALSEDYVVFTEYLDNIAQIAGQPKIHRSVIANKGQIDGNVSFYMRDPIKIE
                     DKYLFYRSYEEGDIIVNPSKWEPEGWVGVPTARDFDIAELNEWMSEGVMPIGVTDRRV
                     SFDGANESAWGFFDHDRIHLDDVPPYNTRIPGFSPKSYFELFRKFGAQFRDTMTQKNL
                     EVIWFYFYHELGPNRGIYSYNPSDLIKSVADPRQRDRVVRGIVSRIQSKDDLGSFYGD
                     QLPTVKHIDEALDELLHFAKNLEKDKTIPALPLTPAQN"
     gene            complement(1240..2319)
                     /locus_tag="PLH57_03335"
     CDS             complement(1240..2319)
                     /locus_tag="PLH57_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRK01672.1"
                     /translation="MFAKYLAVVFEAVRLNIPILVVILATAVVVRLLVFYVLERYIGQ
                     KINALPKNVSLRRHFFPSFLFLLIGLALIATFRKFRIEPEYEANIARVIRLFTIFSAA
                     WFSARIVKLGRIILFENLDITITDNLVERSRRTQIIFIEKMLLLMVWFIGTGAVLYMF
                     EGARKFGASLLASAGVAGVVIGFAAQRSLSNVLAGFQIAFTQPIRIDDVVVVEKEWGK
                     IEEITLTYVVVRLWDLRRLILPITYFAEKPFENWTRTSSQMITTAFIYVDYCVSVEEL
                     RVEFRKVLRATPLWDGDVAELQVTGTTERTVEVRTIMSAADSSKAFDLKCYVREKLIS
                     YMQTQRPQHLPKSRIHVEHDTRLPS"
     gene            2598..4427
                     /locus_tag="PLH57_03340"
     CDS             2598..4427
                     /locus_tag="PLH57_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01673.1"
                     /translation="MFKTHNKSLKQIVLTLGVVSMLLSSSVWAQKTSRMLRVAGKQPA
                     LGQGYDPQTENFKFQCIKSENYAYSPVAQSTLGVGIEISNSALETEFGFETKSRARLG
                     LSKVSAQSDYLKKTRTSNYSHSVHYKFDGKASQVSFDQQAKSGLDLLTPQAVKLLDTK
                     GNLPKITADFITLCGSEVVTAIDRGFKLYVSVSVSFSDSSSFAKFGGSASLSGGWGSI
                     EATIKNLSHETRRNGSISVIAYQLGGSPDELSKIFKSRSGTSNIISCSMSNIDACKSV
                     FSDVFDYASGEFIQQTVSKDYSPKTSISGVANLVYYTEDWQVFGIQTTKTVSNSELDR
                     QRKNYFKLYEDELKKSTAVKSSAQSGVAILSKEERVLYEKAQEIIEDNIEEILSKEQN
                     CFDIDSTDDKTKAAEYCKKAVNALTKHISDSMKNVPDHVFLSAQDKCGSSYCKSCVNL
                     GTFSKPKFTCLKCEATINDIYGKAFELSMSSTHPTFTCNNMPTGKTVTVSVDGKHGNC
                     GWKGTSHGNWYGEMFLNIIEPSGYSGDSAYIRAENQTGARHRANATMSVTVGDSGTIT
                     ADLSDGTCQAANTTVDQQIQVLDPDFYIGIAVTDSDLKQLGGF"
BASE COUNT         1359 a         1024 c          970 g         1094 t
ORIGIN      
        1 atttgaaacg atccgtgagt taaagaaaca aggtgtttct gccgccgacc gccgcagagc
       61 agtcctacca aagctatccg caacaacagt gcgagcacgc tttgatgatg caaagaaaag
      121 cggaaaaaaa gagatttacc tacccgaact tgtgcctgat gttcgttggg cattagcctc
      181 attgaaagaa cgacatgacg ctgcaattcg caagtcgccg tttactcctg agcaaataaa
      241 ccaccttgaa gaggccgcag aattttatcg cctacgattt agctcgatct tagacgaccc
      301 aagaggcgta ccaatgaggg actggttggc acttagcgaa gattacgtcg tattcactga
      361 atatctagac aacatcgcac aaatagccgg acaacctaag attcaccgaa gtgtaattgc
      421 gaacaaaggg caaatagatg gcaacgtctc attctacatg cgagatccaa taaaaataga
      481 ggacaaatac ctattctacc gtagttacga agaaggtgac atcatcgtta atcccagtaa
      541 atgggaacct gagggctggg tgggtgtgcc aacagccaga gattttgaca tcgccgaact
      601 aaacgaatgg atgagcgaag gcgtcatgcc gatcggcgta accgacagac gtgtaagctt
      661 tgatggcgct aacgagtctg cctggggttt ctttgatcat gatcgtattc atcttgatga
      721 tgttccgcct tacaacacgc gtattcccgg cttttcgccc aaatcatact tcgaattatt
      781 tcggaagttc ggggcccaat ttcgggacac aatgactcaa aaaaacctag aggtcatttg
      841 gttctacttc taccacgaac tgggacctaa taggggcata tactcttaca acccgtcaga
      901 ccttattaag agcgtagcag atcctagaca acgagaccgc gttgttaggg gaatcgtttc
      961 tcgcattcaa tcaaaagatg atcttggttc tttttatggt gatcaactac caaccgtaaa
     1021 acacatcgac gaggcgctcg acgaactttt gcactttgcg aagaatttag aaaaagacaa
     1081 aaccattcca gctttgccgt taacgccagc gcaaaattag attgcacaga gaactcctga
     1141 tatttttcag gatcaaattt tacaaaacct cagctgaatt gccagataag ggccatattc
     1201 tttcggtcga agcaccgata gatcaataac aggaggcctc taagaaggta atcgcgtgtc
     1261 atgttcaacg tgaatgcgac tcttcggcaa gtgctgcggg cgctgggttt gcatatagga
     1321 aatcaatttt tcacgcacgt aacatttcaa atcaaatgct ttcgaggaat cggctgcact
     1381 catgatcgtg cgtacttcaa cggtgcgctc tgtagtaccc gtcacctgaa gttcggcgac
     1441 gtctccgtcc caaaggggag tggctctgag cactttccta aactcaactc gaagctcttc
     1501 gactgaaacg cagtaatcta cgtaaataaa cgccgtagtg atcatttgcg acgaggtgcg
     1561 agtccagttt tcaaaaggct tctcggcaaa ataggtaatc ggtaagataa gtctgcgtaa
     1621 gtcccagagt cttacaacaa cgtaggtcaa cgtaatttct tcaatcttcc cccactcttt
     1681 ttcaaccacc acgacgtcgt cgatacggat gggctgggta aatgcaatct gaaaaccagc
     1741 aagcacattt gaaagtgacc gttgcgcggc aaaaccaata accactcccg caacgccggc
     1801 ggacgcgagc agactcgccc caaactttcg tgccccttca aacatataga ggactgcccc
     1861 cgttccaatg aaccacacca tgagcagtaa cattttttcg ataaatataa tttgggtacg
     1921 acgagaacgt tcgaccagat tgtcggtaat cgtaatatct agattttcaa ataagataat
     1981 tcgtccaagt tttacgattc tagcgctaaa ccacgccgcg ctaaaaattg tgaagagccg
     2041 aataactctc gcaatattcg cttcgtactc aggttcaatt cgaaattttc tgaaagtcgc
     2101 gatcaaggcc agaccaataa gtaaaaataa aaatgacgga aagaaatggc gtctcagaga
     2161 gacattcttg ggcaaggcat ttattttttg gcctatatag cgctcaagaa cataaaatac
     2221 caaaagcctt acaaccaccg ccgttgcaag aattactacg agtattggaa tatttagcct
     2281 gactgcttca aaaacgaccg cgagatattt ggcaaacatc tcctcattat aacagcgcag
     2341 ttaatgttga aaacagttgg tataaagcgc tcggaggtaa ccacttcgaa acggggcaaa
     2401 taatatcacg atgaaaaatt ttgccctctg ttccgagcag attagcgaac gtatcttttt
     2461 ccggaattac ggacaaggac cgtacttaat tccggaactg cactaagtaa tttcaagtac
     2521 ttaccccact ttttcgactg gaaccacact tgcacttctt gggcacaaca cgtttattac
     2581 aatggagaat tgtttgtatg tttaagaccc acaataagtc cctaaaacaa atcgtactta
     2641 cactaggcgt cgtctcgatg cttctttcct cttcggtctg ggctcagaag actagccgaa
     2701 tgttgagggt ggctggaaaa cagcccgcgt tagggcaagg ctatgatccc caaaccgaga
     2761 actttaaatt tcagtgcatc aagtcggaga attacgcgta ttcccccgtt gctcaaagca
     2821 ccctcggcgt tggaatcgaa attagcaaca gcgcattaga aactgagttt ggatttgaaa
     2881 ctaaatctcg cgctcgacta ggtctcagca aagtaagtgc tcagagcgat tatttaaaga
     2941 aaacacgtac gtcgaactac agtcactctg ttcactataa atttgacgga aaggcgtcac
     3001 aagtaagttt tgatcaacag gcaaagagcg gattggattt acttacgccg caagcagtta
     3061 aactcctaga cactaaaggc aacttaccaa agattactgc cgactttatt acactatgcg
     3121 gcagtgaagt agtaaccgct atcgatcgag gctttaagct ttatgtgtca gtttcggttt
     3181 cgtttagcga ctcttcgagt ttcgctaaat tcggtggttc cgcttcactt agcgggggct
     3241 ggggaagtat cgaagcaacc attaaaaact tgagccacga aaccagacgc aacggaagca
     3301 tttcggttat tgcctatcaa ttaggtggaa gtccggacga actttccaag attttcaaat
     3361 cacgaagtgg aacatcaaat ataatttctt gttcgatgag caatatcgac gcgtgtaagt
     3421 cggtattttc ggacgtattc gactacgctt ctggcgaatt tatccaacaa accgtaagca
     3481 aggattatag tccaaaaact agtatatcgg gagtggccaa cctcgtttat tacactgaag
     3541 actggcaagt gtttggcatt cagaccacca aaaccgtaag caacagtgaa ctcgatagac
     3601 aacgtaagaa ttattttaaa ctttacgagg acgagcttaa gaaatctaca gcggtaaaat
     3661 cttcggctca aagcggcgtg gccattcttt ctaaagaaga acgagttctg tatgaaaaag
     3721 cgcaagaaat aattgaagac aatattgaag agatcctttc aaaagaacag aactgcttcg
     3781 atatcgactc cacggacgac aaaactaaag ccgcagaata ctgcaaaaaa gcagttaatg
     3841 ccttaaccaa gcacattagc gattcgatga aaaatgtacc tgatcacgtt tttctttcag
     3901 ctcaggacaa atgcggatct tcgtactgta agtcttgcgt gaatcttggg acattttcta
     3961 aacccaaatt tacctgccta aagtgcgaag caacgataaa cgacatttac ggaaaagcat
     4021 tcgaactgag catgagttct actcacccca ctttcacttg caataacatg cccaccggta
     4081 aaacagttac cgtgtcggta gatggcaaac atggaaactg cggttggaaa ggcacttcac
     4141 atgggaattg gtatggagaa atgttcctaa atataataga gccgtccggt tactccggcg
     4201 actcggccta tatccgtgcg gaaaaccaaa ccggggcgcg acatcgggcg aatgcaacca
     4261 tgtctgttac ggtcggagat agcggaacta tcacagccga cctttcggac ggaacttgcc
     4321 aagcggcaaa cacgacggtc gaccaacaga tccaagtttt agacccggat ttttacattg
     4381 gcatcgccgt aaccgatagt gacctaaaac aattgggggg attctaagaa aggggcctaa
     4441 cctgatt
//
LOCUS       DAOMXO010000109         4442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10546_length_4442_cov_6.170276, whole genome shotgun sequence.
ACCESSION   DAOMXO010000109 DAOMXO010000000
VERSION     DAOMXO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10546_length_4442_cov_6.170276"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PLH57_03345"
     CDS             <1..700
                     /locus_tag="PLH57_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRK01674.1"
                     /translation="IAAKVFGTSGYSDYATITEAVAYCNSVGADVINMSLGGGSVYPP
                     LVEEIQHAIKQGTVVVLAAGNSGRGEEHDPKDPIDNVAFPGSIPEVLTVSASDALNRF
                     AEFSSEGPRIDIMAPGTFSFTWISTGYWPTYEDKGIYSSIAGGATVGSSYDGDDDKID
                     GKRRFGRMSGTSMAAPHVAGAAALLKGLNNSASSEQVSDALIESAQRISGQPTNRQGA
                     GLLDVQAAYELLKN"
     gene            713..2983
                     /locus_tag="PLH57_03350"
     CDS             713..2983
                     /locus_tag="PLH57_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01675.1"
                     /translation="MRRKAFGALTSLLVIAVAYLQLGMASCDKTPTVSAPALSSVWLS
                     ASEEAFLIEPPRGFLGSTNRPRIKGQAPSNSTVHLYLGSKCEGAPFPAVSANDFATLG
                     VVAMVSQRSLTMFSAIAENSDGYRSQCSTQIVSYLHDPDAPALPSQLVTEPSVASNNA
                     YPKIKGTIEPNGRVEIYVNSSTCGGTPVRAGADEDGYFEARVEVTRNSVSRIFVRVFD
                     LAENGSLCGPSGGVSYEHDSNVELTLVDTTPPSPSNNPSPTLNGLAEAGTNIWLYDTA
                     DCSGNPIGSGMSDGTGRFSIPFAPRLPATGTQGVVTTTIKAKAQDAVGNLINCAKPSV
                     SYTFDNAPPMAPILSGTLPSGSSSDETPLVYGTGVEPNSFIELFRGAPGDPLCSGMPL
                     NSPTLGNADGSFSVAVTVPENSTSVLSARVRDRAGNSSPCSNGLVYLEDSTAEPIVFI
                     SPTIPESPSNQASFRVFGLIEPNSSIALYSSSSCEASTIVGSGSTADLAGSGVLVTLP
                     NDLLNQTVSIHGIVTDEFNNVSTCSTNSLVYIHDSTPPDAPILFLASSLGNNNSVLVN
                     GNGPNELHPGYAEIFKTNNCTGPVATSVPITANPTGFDFSAVITVPDNSVTSFSARHI
                     DAAGNPSACGASLSAYREDSLAPITPQLLGLLPASPSTTGHITASVYQPDVARFFGEF
                     YRVRLTGSGGPCTSSAYFAGQSAPLTQPVLLSVAGDLGLFGVYRVRAFTIDGAGNSSA
                     CSNEEFEFIYEPELMP"
     gene            complement(2990..4126)
                     /locus_tag="PLH57_03355"
     CDS             complement(2990..4126)
                     /locus_tag="PLH57_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01676.1"
                     /translation="MMTLLWTFPAIMLASIIIGWAAEVTAIYLSAGIALAVLAWLQTA
                     PEFAVEATIAWQQNSHLALANLTGSLRLLMGFGWPMIMFIHWYSTRKRRKNALKLGDV
                     TLPKSFSVEAAGLLVPVVYFLVIYLKKTWSFYDGVILCSFYGFYFWLLNRQRKVGFHV
                     DEIEDDAPEPWVVEKVRKLPGISRPLAVIGFFVVGGGVLLITVHPFVDALKASALALG
                     ISEFVFIQWLAPIASEFPEKVTAFNWARRANKVPMAVVNMLSSVTSQWTLLAGLVPVV
                     FSISAGHAVTLELSDFQRTELLLTIAQSLLAVLFLSDLKIRDIEAMGLFTLWFVQFLM
                     PTTREPLVVIYAIWIVFEASRLVLRGGNSFVAWKTVGAILRGRK"
     gene            4190..4264
                     /locus_tag="PLH57_03360"
     tRNA            4190..4264
                     /locus_tag="PLH57_03360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4223..4225,aa:Arg,seq:ccg)
BASE COUNT         1145 a         1132 c         1066 g         1099 t
ORIGIN      
        1 aattgctgcg aaggtgtttg gtacgagtgg ctacagcgac tacgcgacca tcaccgaggc
       61 cgtcgcttac tgcaactctg ttggcgccga cgtaattaat atgagtttag gcggaggtag
      121 cgtttaccca cccctagtgg aagagattca acacgctata aagcaaggca cggttgttgt
      181 tcttgcagcg gggaattccg gacgcggcga agaacatgac ccgaaggatc caattgataa
      241 tgttgccttt ccagggagca ttcccgaagt tttaactgta tcggcgtccg atgcattaaa
      301 tcgtttcgcc gaattttcga gcgaaggtcc ccgtatcgat attatggcgc ctggcacttt
      361 ttcgtttacc tggatttcga ccggttactg gccaacctac gaagataaag gaatttactc
      421 ctcgatcgct ggtggggcca cggttggttc tagttacgat ggcgacgacg acaaaatcga
      481 cggaaagcgc cggtttggta gaatgtcggg cacctctatg gccgctccgc acgttgccgg
      541 tgcagctgct ttgcttaagg gcttaaataa cagcgcctcg agtgaacaag tctctgacgc
      601 actaatcgaa tccgctcaga gaatcagtgg ccaaccgaca aaccgacagg gcgcaggttt
      661 gctagacgtg caagctgcct acgaactgct taagaactaa gggtctcctt ctatgaggcg
      721 caaagctttt ggggcactaa caagcttact ggtgatcgcg gttgcctact tgcaacttgg
      781 catggcgtct tgcgataaaa cacctaccgt tagcgcccct gcgctatcta gcgtgtggct
      841 ttctgcatct gaggaggcat ttttaattga accacctcga ggctttttag ggtccactaa
      901 tcgtccgcga attaaagggc aggctccgag caatagcact gttcatttgt atttaggcag
      961 caaatgcgaa ggagcaccct ttccagcagt atcggccaat gactttgcaa ctcttggcgt
     1021 cgttgctatg gtgtctcagc gctcgctcac aatgttttca gcaattgccg aaaacagtga
     1081 tggctatcga tcccagtgtt ccactcaaat tgtttcatat cttcatgacc cagacgcgcc
     1141 tgctctccct tcgcaactcg taaccgaacc aagtgtagcc tcaaataacg cttatccaaa
     1201 aataaaggga accatagaac cgaatgggcg agtagaaatt tatgttaaca gcagtacttg
     1261 cggtggaact cctgtgcggg caggggctga tgaagacggc tactttgagg cccgcgtaga
     1321 agtaaccaga aactctgtta gccgtatttt tgttcgggtc ttcgacttgg cagagaacgg
     1381 atcgctttgc ggccctagtg gtggtgtaag ttacgaacat gattcgaatg ttgaactaac
     1441 tttagtagac acaactcctc cgtcaccatc caacaacccg tcgccgactc tcaatggttt
     1501 agcggaagct ggaactaata tatggctgta cgacactgcg gactgtagcg gaaaccctat
     1561 tggatcaggc atgtctgacg gcacggggcg ctttagtatc ccgtttgcac cgcggttacc
     1621 ggccacggga actcaaggcg tcgtaaccac taccattaag gcgaaagctc aagacgctgt
     1681 aggaaactta ataaattgtg caaaaccttc agtttcgtac accttcgata acgcgcctcc
     1741 aatggctcca attttatctg gaactttgcc cagtggttct tcgtccgatg aaactccgct
     1801 agtttatggc accggcgtag agccaaatag ttttattgag cttttccgtg gagctcccgg
     1861 cgacccactc tgttcgggta tgccactcaa tagtccgact ctcgggaacg ctgatggttc
     1921 gttttcagtg gcggtaacag ttccagaaaa ttctacctcg gtactatccg cgcgtgtgcg
     1981 agatcgcgca ggtaattctt ctccatgtag taatggattg gtctatctag aagattccac
     2041 agcagaaccc atagtgttta tttcaccaac gatccccgag tctccttcga atcaagcaag
     2101 ctttcgagta ttcgggctta tagagccaaa ctctagcatt gcactatata gttcttcgag
     2161 ttgtgaggcc tcgacaatcg tgggcagtgg aagcactgcc gatttagcgg gatcaggcgt
     2221 tttagttact ttgccaaatg atttactaaa tcaaaccgtc tctattcatg gaatcgtcac
     2281 ggacgagttc aataatgttt caacgtgttc gacgaattcg ttggtataca ttcacgactc
     2341 tacgcccccc gacgcaccca tactttttct cgcttctagt ctaggaaaca ataattcggt
     2401 cttggtgaat gggaatggcc ccaacgaatt acatccaggg tacgctgaaa tttttaaaac
     2461 caacaactgc actggacctg tcgctacgtc tgttccgatc acggcaaatc cgacgggatt
     2521 tgatttttcc gcagtaatta cagttcccga caattcggtc acatcgtttt ccgcaaggca
     2581 catagacgca gctggcaacc cttcggcgtg tggggcatcg ctctcggcct atcgagaaga
     2641 ttcattggca ccgatcactc cacagctact ggggctactg cccgcgagcc ccagtacaac
     2701 tggacacatt acagcttcag tttaccaacc tgacgtcgcc cgattttttg gcgaatttta
     2761 tcgcgttcga ctcaccggca gtggcgggcc ttgcacttcc agcgcttact tcgcgggtca
     2821 aagtgcacct ttgactcagc cagtattact ttcggttgcc ggagatctcg gcctgttcgg
     2881 tgtctaccgg gttcgtgctt tcacgatcga cggcgctgga aattcttcag cttgtagtaa
     2941 cgaagagttc gagtttattt acgaaccaga actcatgcct taatgtaaat cactttcggc
     3001 cccgaagaat agcgcctacg gttttccaag caacaaagga atttccacct cgcaacacaa
     3061 gccgagacgc ctcaaaaact atccagatcg cgtaaattac tactagtggc tctcgcgtcg
     3121 tgggcattaa aaactgcacg aaccagagag tgaacaagcc catagcctcg atgtctctaa
     3181 ttttcagatc agatagaaac aatactgcca aaagtgactg cgcaatggtt aacagcaatt
     3241 cagttcgttg aaagtctgac aactcaagcg taacggcgtg acccgcagaa atagaaaata
     3301 cgactggtac gaggccagcg agcaaagtcc actgactggt gacagacgaa agcatattca
     3361 ccactgccat gggaactttg ttggcgcggc gcgcccaatt gaatgcggta actttctcgg
     3421 gaaactctga cgctatgggt gctaaccact gaataaagac gaactcacta attccgagtg
     3481 ctaaggcact agctttaagg gcatcgacga acggatgcac cgttattaaa agcactcctc
     3541 ccccaaccac gaaaaaacca atcactgcga gtggtcgaga tataccggga agttttctga
     3601 ctttttcaac aacccacggt tcgggcgcgt catcttcgat ttcatcgaca tggaaaccta
     3661 ccttgcgttg gcggtttaac aaccaaaaat aaaatccata aaaactacat agaattacgc
     3721 cgtcgtagaa ggaccacgtt ttttttagat atataactag aaaatatacg accggcacca
     3781 gtagacctgc cgcttccacc gaaaaagact taggaagcgt aacatccccg agtttcaggg
     3841 cattttttcg cctttttcgc gtggaatacc aatgaataaa cataatcatt ggccaaccaa
     3901 agcccatcag aagtcgcaag ctgccggtaa gattcgccag cgcgaggtga gagttctgtt
     3961 gccaagcaat ggtcgcttca actgcaaatt caggagcggt ctgaagccac gcgagaaccg
     4021 ccagcgctat acctgcagac agataaatgg cggtaacttc tgcggcccaa ccaataatga
     4081 tcgacgcgag catgattgcg ggaaaggtcc ataaaagagt catcataata ggaaatagat
     4141 aagggacgac ttgaattcag tccactactg atttaatcgg tctaggcttg gtttcgtggt
     4201 gtaatggata acacgccacc ctccgaaggt ggaaatacag gttcgattcc tgtcgaaact
     4261 accagttctt gctctttttc cctcgtacat cgtcagaaaa taaaatgctc ctgcgactag
     4321 ttatctaatg tcgagacgcg tgagtgtctc gtaccttacg gcaggaatcg aaagccggaa
     4381 gacgccggtt acccgctttg cgggtggcgt ctgaggccgg cctgcagccg aggcgccaaa
     4441 gg
//
LOCUS       DAOMXO010000110         4411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10647_length_4411_cov_4.711433, whole genome shotgun sequence.
ACCESSION   DAOMXO010000110 DAOMXO010000000
VERSION     DAOMXO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10647_length_4411_cov_4.711433"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1099
                     /locus_tag="PLH57_03365"
     CDS             83..1099
                     /locus_tag="PLH57_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRK01677.1"
                     /translation="MHQKKILIIRFSALGDVVLALRSLRGVDSSALEIHFLTKKKFGG
                     IVAKSNWVQRVHTISDRASLSELNRQMQGLKKENFDAVVDLHSVMRSRAACALLGIKT
                     FRAHKPRIIEWVLFLLKQWPFKKIGLRPLDRVALFDRTLDRTLNFLNLSRVRNKEECD
                     FGGLEDIKKKHVAIAADSAWSQKEWPIERFANLALRLVERGESVVWLGFKKLPRSIPG
                     VSDLSGTTRLEDLPKILAESKILLCNDAGLMHLAESVGTPVVAVFGPTVPQLGFQPHR
                     LESRVLESTLWCRPCSKSGRWCFRPLEPQLCLKLVSEDRVWQALSAILRDTKQNSNEA
                     SQSI"
     gene            1216..2382
                     /locus_tag="PLH57_03370"
     CDS             1216..2382
                     /locus_tag="PLH57_03370"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRK01678.1"
                     /translation="MSLRFHANTPIEYWIHVASAGELEYAMPILNELNLIGKKVLVTY
                     YSVSAQIPVEALPARLPCVALTVPLPHDGLGLMGEFVKLVRKQGVHTLLLLKYEMWPG
                     LLWECNAHGVRVILAEALKPSWFHRKLIHKLSALLAGYETELEGVDHPHRFVVGDTRV
                     ERVNERLGSEEPLLVKKLRDILGTRRAVVCGSMWPEDHEVLTNGLKKIVETKTVGSSN
                     SLPDLIWLPHELDRKFPQKAESDLKQIGYDTLWVEKDADLTQVRLSDRPAAFIVFKKG
                     VLVELYKLGRCAFVGGGFVSNVHNVWEPALAGCRVACGPEISRAPEASQLSSEGLLKV
                     LTDASSMSLWLKQAVCTNESGRQDEEVRTRESLARVVETHRGAARRIVDYCEGQ"
     gene            2379..2798
                     /locus_tag="PLH57_03375"
     CDS             2379..2798
                     /locus_tag="PLH57_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRK01679.1"
                     /translation="MKDQKENLRVKIRLRMSSHDAHYAGNLVDGARMLGLFGDVATEL
                     LIRLDGDEGLFRAYESVEFLAPVQAGDYIEAEGWITEVGNTSRRMEFEARKVIAPVPA
                     GDPEYRAPSSARVLSEPVVVCRAKGTCVTPKTLQRKK"
     gene            2800..4356
                     /locus_tag="PLH57_03380"
     CDS             2800..4356
                     /locus_tag="PLH57_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01680.1"
                     /translation="MQISERYDVICVGGTFSSYLAAAILSTAGCRVLVIDPQEGCVPH
                     DVDGRTIFDPDLTPIWGLEFDSSLSRTLSNLGILGTPDALPYVSLGGLSQVLNPAHRV
                     KLSFDSNKLLQELKLEIPDSHGVLTELFEKLVQAAHEIPRYSSLVQNPAQMASQELAK
                     TLKFWGVYGAAALGKDQALHDLLGKKSSIANAFLSGLIATLGPVSSKNVGTEQVLRTL
                     SVAIRGQAFWRGGVESLKATLRRVAVQNGTHLVLRAQVDGLLYEKDRVRGVQLSSYDG
                     VVFADYTILTTHLRKTYQTLPSVVADPTVMRSLNRVVPSHWRYTIAVSLNRDVIPVGA
                     TELMSYIGSYKLELEEENLLRIVVVPEGVYVDADTPDTVTVHVTALVPYRVSSLDYGY
                     LRRLGGKMLHALAEIIPFFEYNVVSMYPDIRKDDRDLRALYPFDRREWVSEDLLSYYV
                     RGGQECQAFAGVSWATPHENLYFAGRAALPSLGVFGEAVVTEKIVKDLLSPVRWKAPP
                     EPTVLKESSL"
BASE COUNT         1110 a         1009 c         1149 g         1143 t
ORIGIN      
        1 tcggttggct cctttgcctc ccggggcgtt tgtgcttttg tacattgctt ttgatcgggt
       61 tttcctagaa tacttttaga gcgtgcatca aaaaaaaatt cttatcattc gatttagtgc
      121 cctgggcgac gtcgtccttg ctctgcgttc attgcgaggc gtggactcta gcgcattaga
      181 aattcatttc cttaccaaaa aaaaattcgg tggcatcgta gcaaagtcga actgggtcca
      241 acgcgtgcac accatttcgg accgggcttc gctgtcagaa ctaaaccgcc aaatgcaagg
      301 tttgaaaaaa gaaaatttcg acgccgtcgt agacctgcat tcggtaatgc gttcgcgagc
      361 ggcgtgcgcg cttctcggta ttaagacatt ccgggcccat aaaccgcgaa tcattgagtg
      421 ggtgctattc cttttgaaac aatggccctt taaaaaaatc ggtctgcgac cgcttgatcg
      481 cgtcgcgctt tttgaccgca cacttgatcg aacattaaat tttttaaatc tctcacgcgt
      541 aagaaataaa gaggaatgtg atttcggcgg gctcgaggat atcaaaaaaa aacatgtcgc
      601 catagccgcc gattccgctt ggtctcagaa agaatggccg attgaaagat ttgcaaatct
      661 cgcgctgcgg ttggtggagc gcggagagtc tgtagtctgg cttggtttta aaaaactgcc
      721 aagatcaata ccgggggtct ctgatctttc tggtacgaca cggcttgaag atctcccgaa
      781 aatactcgcc gaatccaaaa tccttttgtg caatgacgct ggactaatgc atttagccga
      841 aagtgtcggt acaccagtcg tagcggtatt tgggccgacc gttcctcaac tcggatttca
      901 accgcaccgt ttagaaagtc gggtgttgga gagcacgttg tggtgcaggc catgctctaa
      961 atccggtcgt tggtgttttc gacccctcga gcctcaattg tgcttgaaac tcgtgtccga
     1021 agaccgcgtg tggcaagcac tgtctgctat attgcgcgat acaaagcaaa actctaacga
     1081 ggcgtcgcaa tcgatatgaa gacttggatt tattcatgga ttgtctatcc gctcgctctc
     1141 ggaataggga tgattttcgg gatttttcgc tcaaaggtgc ggcgtactct acgtttgaga
     1201 tcctggaaga atcacttgag tcttagattt catgccaaca caccgatcga gtactggatc
     1261 cacgtggcca gcgccggaga gctcgaatac gcaatgccga ttttaaatga gctcaatcta
     1321 ataggaaaaa aagtgctcgt tacctactac tcggtgtccg cgcagatacc cgttgaagct
     1381 ctgcccgcac gccttccttg tgttgcacta actgtacctc ttccacacga tggcctgggt
     1441 ttaatgggtg agttcgtaaa acttgtgcgt aaacaaggag tccacactct cttgttgttg
     1501 aaatacgaaa tgtggccggg gttgctgtgg gagtgtaacg cccacggagt tcgcgtcatt
     1561 cttgcggaag cattaaaacc tagttggttt catcgaaaac tgattcacaa actatctgcg
     1621 ctactcgcgg gttacgagac cgagttggag ggggttgatc accctcatcg ttttgtcgtt
     1681 ggagatacaa gagtcgagcg agtgaacgaa cgtttagggt ccgaggaacc attattggtt
     1741 aaaaaattgc gggatatcct tggaacaagg cgcgcagtag tttgtggcag tatgtggcca
     1801 gaagaccatg aagtgttaac caatggctta aaaaaaatcg tagaaacgaa aaccgtcggg
     1861 tcgtctaatt cattgccaga tttaatttgg ctccctcacg agcttgatcg taaattcccc
     1921 cagaaagcag aaagtgattt aaagcaaatc ggatacgaca ctttatgggt cgagaaggac
     1981 gccgatctaa cgcaagtacg cttgtcagat cgccccgcag cttttatcgt ttttaagaaa
     2041 ggcgttttag ttgagcttta taaactgggt cgatgtgcgt tcgttggtgg tggttttgtg
     2101 tcgaacgtac acaatgtttg ggaacctgct ctggctgggt gtcgtgttgc ttgtggaccc
     2161 gaaatcagtc gtgcaccgga agcaagccag ttatcttcgg agggtttgct aaaagtgttg
     2221 accgatgcgt cgagcatgag tttgtggttg aaacaagcgg tttgcaccaa tgagagcggc
     2281 cgccaggatg aagaagtgcg aacccgggaa agcttggctc gggttgtcga aacgcatcgc
     2341 ggagctgcgc gtcgaattgt ggactattgc gagggacaat gaaggatcag aaagaaaacc
     2401 ttagagtgaa gattcgattg agaatgagtt cgcacgatgc acactatgcc ggaaatctag
     2461 tcgacggcgc gcgaatgctc ggtttgttcg gcgacgtcgc aacggaactt ctcattcgat
     2521 tagatggtga tgaaggtcta tttagagcct acgagagcgt cgaatttctg gctcccgtac
     2581 aagctggtga ttatattgaa gctgaaggtt ggattactga agttggcaac acatcgaggc
     2641 gcatggagtt tgaagcccga aaagtgatcg ctccggttcc agctggtgac ccagagtacc
     2701 gagccccttc gtcagcccgc gtattatctg aaccagtcgt ggtttgtaga gctaaaggga
     2761 cctgtgtaac tcccaaaaca ttgcagcgaa aaaaataaga tgcaaatatc cgaacgctac
     2821 gacgtgattt gtgtaggcgg cacgttttct tcctatctcg cagcagctat tctgagcacc
     2881 gccggttgtc gcgtattggt gatcgaccca caagagggat gcgtgcctca cgacgtagac
     2941 gggcgaacga tttttgaccc cgatcttact ccgatttggg gactcgaatt cgattcgtcc
     3001 ttatcgcgca cccttagcaa tcttggaatt cttggaacac ccgatgcgct cccttacgtg
     3061 agtcttggag gattgtctca ggtccttaat ccggcgcatc gagtaaaact gtcgtttgac
     3121 tccaataaat tgctgcagga actcaaactc gagattcccg actctcacgg agtcctgacc
     3181 gaattatttg aaaaactcgt tcaggccgcg catgaaattc ctcgctattc atcgttggtt
     3241 caaaatccgg cgcaaatggc ttcacaggaa ttagctaaaa cattaaagtt ttggggcgtc
     3301 tacggtgccg ctgctctagg gaaagaccaa gccttacatg acctcctggg gaaaaaaagc
     3361 tcaatagcga atgccttcct tagtggtcta attgcaacgc ttggaccagt gtcttcaaaa
     3421 aacgtcggta ccgaacaggt gttaagaacg ttgagtgtcg cgatccgagg ccaagctttt
     3481 tggcgaggag gagtggagtc gcttaaggct acacttcgtc gagtagcagt gcaaaacgga
     3541 acgcatctcg ttcttcgagc acaagttgat ggactgctgt atgaaaaaga tcgagtgcgt
     3601 ggagtgcagt tgtcctctta cgacggtgta gtgttcgcag actacacgat tttgacgact
     3661 catttgcgca agacctatca gaccctcccg tcggtagtgg ccgacccaac agtcatgcga
     3721 agtttaaatc gcgttgtacc gtcgcattgg cggtatacga tcgctgtgtc gttgaatcga
     3781 gacgttattc ccgtcggtgc taccgaacta atgtcttaca ttggttccta caagctagag
     3841 ctcgaagaag aaaatctact gaggatcgta gtggtgcccg aaggcgttta tgtcgacgct
     3901 gacactcccg acactgtaac ggtgcacgtg accgcattgg tgccttatcg agtatcaagc
     3961 cttgattacg ggtatctaag acgcctcggt ggcaaaatgc tccacgcttt agcagagatc
     4021 attccattct ttgaatacaa tgtcgttagt atgtatccgg atattcgcaa agacgatcgc
     4081 gatttacgag cgctctatcc gtttgaccgc cgcgaatggg tgtccgaaga tttattgtcg
     4141 tattacgtgc gaggaggtca agagtgccaa gcttttgctg gcgtgtcgtg ggccacacca
     4201 cacgagaatc tctattttgc cggacgtgcc gcgctaccta gtttgggagt tttcggtgag
     4261 gcagtggtaa cagaaaagat tgtaaaagac cttctttcac cagttcgctg gaaagcgccc
     4321 cccgaaccaa cagtattaaa ggaatcaagc ctatgatcga aagatacaca ctaaaaaaaa
     4381 tgggtgaaat ttggactgac gaatcaaagt t
//
LOCUS       DAOMXO010000111         4407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10663_length_4407_cov_4.938189, whole genome shotgun sequence.
ACCESSION   DAOMXO010000111 DAOMXO010000000
VERSION     DAOMXO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10663_length_4407_cov_4.938189"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLH57_03385"
     CDS             complement(<1..472)
                     /locus_tag="PLH57_03385"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRK01681.1"
                     /translation="MNLNEKVSAELLTDLKKYGDRTSSFMTLYPGFESYKSDNGLVRY
                     METTSAWVAGTEPLAPAEKKIDVFNAFKNSALNKRKNAIVAPVTEEFSKELTKAGFHR
                     IQIGSEPVFDLTSYFSTIDPVHRFPSARSLQKRGAVVRELAFESMTQEQRDRILM"
     gene            complement(459..1601)
                     /gene="ddlA"
                     /locus_tag="PLH57_03390"
     CDS             complement(459..1601)
                     /gene="ddlA"
                     /locus_tag="PLH57_03390"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRK01682.1"
                     /translation="MQQKKAKLRVGILFGGKSGEHEVSVTSALSIFQALDKEKYEVTL
                     VGIDKTGRWLLPEQAHVLAHQKNPRLLKLNEVRETVGFLPFPAEKNLVPFEGGKLNAG
                     SASQFDVVFPVLHGTNGEDGTMQGLLELANVAYVGSGVLGSSVGMDKDVSKRLFRDAG
                     IPVVPYLMLHRHEYRASQEMAIEEAQRKFGYPYFVKPANLGSSVGVGKVKNKTDAIAK
                     IEDAFQYDTKILIEQAIDARELECAVLGNHQPEASVVGEIIPLHEFYSYEAKYIDAQG
                     ADLRIPAENLSREIEERVRTLAVQGFKALECSGLARVDFFLDRKTGKIFLNEINTIPG
                     FTSISMYPKLWEATGMPYSKLLDRLIHLALERFEEKRGLKTTYESQ"
     gene            1758..1847
                     /locus_tag="PLH57_03395"
     tRNA            1758..1847
                     /locus_tag="PLH57_03395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1792..1794,aa:Ser,seq:gct)
     gene            complement(1955..3178)
                     /locus_tag="PLH57_03400"
     CDS             complement(1955..3178)
                     /locus_tag="PLH57_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRK01683.1"
                     /translation="MKRKLEKVTSCPGVFKIVVFDSETNDWNEPPHGSMYRAFRYEQI
                     HEGKRRKVSKYFNSLTSAKVWALGSHARTEEYKLPLLQKNPSAGMTFGEVVQTWLKVW
                     LPNKQIGTQLRYKAYLKHFEFFWNMPVERIEPSDVDEWITKIKKPEYLKMGHTTRCDY
                     RHEFSVLRNILNFYASRYNRNYRLPFLKDHKAMLKVREKLKARKDLTVDEFRNFIGCL
                     QRICIEHDCEAVYYLALMQYSTYSRIQETAALAVDDFDFSRNKILFTKKMFWPRKRGF
                     QTQVVDGAKAGGGKEIPMSELASKVCKEWMLKSGIRSGQLFMLDGELITYRQIEYRYT
                     RALQQAGLPFSATHILRHASLTEFYQTSKDLLLTAAMAGQRDLRSTTKYTKVRDERVV
                     QMQKQMDEKWAGILS"
     gene            complement(3445..3606)
                     /locus_tag="PLH57_03405"
     CDS             complement(3445..3606)
                     /locus_tag="PLH57_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01684.1"
                     /translation="MSFDSVLYTVGSLDPVGWSHQWSIAHVQEPEPTALKAHINPQDL
                     NAKSGPTSI"
     gene            complement(3596..3982)
                     /locus_tag="PLH57_03410"
     CDS             complement(3596..3982)
                     /locus_tag="PLH57_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01685.1"
                     /translation="MGIKISLGPRQTVVPAGEYLAKLVEIKEGTFVGQRKTFEFLFEI
                     ASGEHSGIIVRGFVNANYETFSEYTKLRQWATMVARFDTDMGAKLDLDIFFGKILKVK
                     VETKVSKKTKNLFSNVTAILGLEYEL"
BASE COUNT         1051 a         1062 c          976 g         1318 t
ORIGIN      
        1 gcattaaaat acgatcgcgc tgctcctgtg tcatgctctc gaatgcaagc tctcgaacca
       61 ccgcgcctct cttttgaagt gaacgggcgc ttgggaagcg gtgaacagga tcaatggttg
      121 aaaaataaga agttaaatca aacaccggtt ctgaaccgat ttgaatgcga tgaaatccag
      181 cttttgtaag ttcttttgaa aattcttcgg tgaccggagc tacgatggcg ttttttcttt
      241 tatttagtgc gctattttta aaagcattga atacatcaat ttttttttcg gcaggcgcca
      301 acggttcggt gccggcaacc caagcggaag ttgtctccat ataacgaacc aaaccattgt
      361 ctgatttata agattcgaat cctggataca atgtcataaa cgaactagta cgatcgccgt
      421 atttttttag atcggttaat agttcggcgc taactttttc attgagattc ataggtcgtc
      481 tttaatccac gcttttcttc gaatctttcg agggcaagat gaataagacg atccagcagt
      541 ttggagtaag gcatgccggt cgcttcccag agtttcgggt acatacttat gctcgtaaaa
      601 cccgggatcg tgttgatttc gtttaaaaat atttttcctg ttttgcggtc taggaaaaaa
      661 tcgacgcgag ctaacccgct acattcaagc gctttaaacc cttgaactgc caacgttcga
      721 acccgctctt caatttcacg cgacagattt tcagccggaa ttcgcagatc ggcaccttgg
      781 gcgtcaatgt acttcgcttc gtagctatag aattcgtgca ggggaataat ttctcccacc
      841 acgctagcct cgggttggtg attaccaagc acggcacact ctagttcgcg tgcgtctatt
      901 gcctgttcaa ttaaaatttt agtgtcgtac tgaaaagcat cttcgatttt ggcgatcgcg
      961 tcggttttat tctttacttt acctacccct accgaagatc cgagattggc tggttttacg
     1021 aaataggggt atccgaattt gcgttgagct tcttcgatcg ccatttcctg ggaggctctg
     1081 tattcgtgtc tatgcagcat caaatagggg accaccggaa ttcccgcgtc acgaaacaat
     1141 cttttggata cgtccttatc cattcccacc gaagatccaa gcacgccgga cccgacatag
     1201 gctacattcg ccagctcgag caagccctgc atggttccgt cttcaccgtt ggtgccgtgt
     1261 aaaaccggaa atacgacatc gaactgagaa gccgatccgg cattgagttt gcccccttcg
     1321 aagggcacca ggtttttttc ggctggaaac ggcagaaatc caacggtttc gcgcacttca
     1381 ttgagtttta gtaaacgtgg atttttctgg tgggcaagta cgtgagcttg ctcgggaagc
     1441 aaccaacgcc cggttttgtc gattcctacc agggttactt cgtatttttc cttatcgagt
     1501 gcttgaaata tagatagtgc ggaagtcacg ctcacttcgt gttcgccgga ttttccacca
     1561 aataatatgc ctacccttaa tttagctttt ttttgttgca tgtcgccgca cgactcctcg
     1621 aattagttta acgggggatt gggaaaattt ccaacgtatg acccaaagtt cgagcattcc
     1681 gtcttccaaa atccgctggg caggggtaaa acagtgtgct accagggtag ggattgccta
     1741 ctcgtcttta ataccttgga gaggtggccg agtggtcgaa ggcactccct tgctaaggga
     1801 gcagggggta aaacccctcg cgagttcgaa tctcgccctc tccgccatat tttgactgat
     1861 gccttgtaat cccaatgatt acgaggcttt tttgtttttg tgcgtaggca aattgctttt
     1921 gagagctgtt tgagagctcg caaaagtgat tactctacga caaaattcca gcccattttt
     1981 cgtccatctg tttttgcatt tggacgactc tttcatctct cacttttgtg tacttagttg
     2041 tggacctcaa atccctttgg cctgccattg ctgcagttag caacaaatct ttgctcgttt
     2101 ggtagaattc cgtgagcgaa gcatgccgca gaatatgcgt ggccgaaaag gggagtcctg
     2161 cttgttgaag tgcccgcgtg taacggtact ctatttgacg gtaagtgata agctcaccgt
     2221 cgagcatgaa aagctgccca cttcgtatgc cagatttcag catccactct ttgcaaactt
     2281 tggatgcaag ttctgacatt ggaatttctt ttcctccacc tgcttttgca ccgtcgacga
     2341 cttgcgtttg aaagccacgc tttctcggcc aaaacatttt ctttgtgaat agaattttgt
     2401 ttcggctgaa gtcgaagtcg tctacagcaa gcgcggcggt ttcttgaatt ctggagtagg
     2461 tgctgtattg cattaacgcc agataataga cagcctcaca atcgtgctct atgcaaatgc
     2521 gttgcaagca accaatgaag tttcgaaact catcaaccgt tagatctttc cgtgctttca
     2581 atttctcgcg aaccttcaac attgctttgt ggtctttgag aaacggaagg cggtagttgc
     2641 ggttgtagcg acttgcgtag aagttcagaa tatttctgag cacgctgaac tcatgcctgt
     2701 aatcgcaacg agtggtgtgc cccattttga gatactctgg tttcttgatc ttcgtgatcc
     2761 attcgtcgac atcgcttggc tcaatgcgtt cgacgggcat attccaaaaa aactcgaaat
     2821 gctttagata ggccttgtac cgaagttgcg ttccgatctg tttatttgga agccacactt
     2881 ttaaccaagt ttgaacgact tctccaaaag tcattccagc gcttggattt ttttgcagga
     2941 gcgggagttt atattcttca gttcgtgcat gagatccaag cgcccaaact ttggctgatg
     3001 tgagcgaatt gaaatacttg ctcacttttc tgcgtttgcc ttcgtggatt tgttcgtacc
     3061 gaaatgctcg gtacatcgaa ccatgcggcg gctcattcca gtcgttcgtt tcactgtcaa
     3121 ataccacgat tttaaaaacc cctggacaag acgtgacctt ctctagttta cgtttcattt
     3181 ttgatttctc tttcctcgat ccaacgagtt aaagagctca tgcgaaaccg tatcgctcga
     3241 ctgcctacaa ctacataagg caattgcccc gatgctttta gtttacgaag atacggctct
     3301 gacaatccaa ggcactgagc ggcttgtttg tatgttagta ggttttcaaa gaccgactcg
     3361 aacccaaacg gtctatcgtg gcgcactggg ccggacaagt tattatcttt catactctta
     3421 ctttcgttaa cccactccca agacctagat gcttgtggga ccgctcttgg catttaggtc
     3481 ttggggattg atgtgggcct ttagggccgt gggttcaggc tcttgaacat gggcaataga
     3541 ccattggtga ctccacccaa ctgggtcaag tgaaccaacg gtgtatagca ccgagtcaaa
     3601 gctcatactc cagccccaaa atcgccgtaa cgttactaaa tagattcttc gtcttttttg
     3661 atacttttgt ttcaactttc actttgagaa ttttgccgaa aaaaatatcc aaatcgagct
     3721 tggcacccat atcggtatca aaccttgcaa ccatcgtggc ccactgccgt agcttcgtgt
     3781 attcggagaa tgtttcgtaa ttcgcgttaa cgaatccgcg cactatgatt ccagagtgct
     3841 ctcccgatgc aatctcaaat aaaaactcga atgtttttcg ctgtccgaca aaggttcctt
     3901 ctttgatttc aactaacttc gccaaatact cgcccgctgg gacaacagtt tgcctagggc
     3961 ctaaactaat tttgatgccc ataactactc cgagcggtag gggcgatact gccaaagtgg
     4021 gcatttccac gttgggcagt tttggattcg catgcttacg gtttcaaatc cgacgcaatc
     4081 aatacacttt gcttttatcg cttgccgggg ggcaactgag ctcaggaaca cttttcggaa
     4141 aagcccttga aaccgcagag gtaatgctcg aacgtacgct aaatgccttt ttgcggccaa
     4201 attctcatcg ccttgatcga aatttttatc gatcgcctct cgttcaccta cgaactctcg
     4261 ttcgcaagct gttttcagaa atccactccc tgatgcattt tttttattat tcatggcaaa
     4321 ccgttacatt ctcggtgatc gcaccaaaga gatcttcttt tcgaacaccc agggtgttag
     4381 ccagcgcatt gatctgatca tcatttg
//
LOCUS       DAOMXO010000112         4399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10701_length_4399_cov_6.124770, whole genome shotgun sequence.
ACCESSION   DAOMXO010000112 DAOMXO010000000
VERSION     DAOMXO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10701_length_4399_cov_6.124770"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1751)
                     /locus_tag="PLH57_03415"
     CDS             complement(111..1751)
                     /locus_tag="PLH57_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRK01686.1"
                     /translation="MDWGTKRSTLKVVWSVVAMAVVVTALTLVLVWRLDRVENRASAV
                     NQYYLPVIRSLNVMQGKWQAYQRTFEQNVGFRRWGARTKPTTVSGRSGLAKAFGSDVT
                     DLEKILQAARSNSNVKNKRDVHFAVVDEVATWLRRVDDISKKETEFFVSLAERLKNKD
                     YTGAALLYSNFRASLTSVFTTEWSDLSHRIELVAQENQHLIESELGTSQSIVLFFLIG
                     TLVLSLIVLLKLNRWVVPVSEWTRVAREIVLRGTHSDIKYPPVRGSMPEEMQILTREF
                     TRMATTLGEREKTINEQKKKLEAAVETERKLAHAKKLLLAANMSSQVAHEVRNPLNSM
                     GLQLEMLKEDLESKIGEGDALLKRVDTVTQQIQRLERITDRYLDLGSVRDQARKYESV
                     DLHALIEDCVEFVAQELAARKIRVELKLDAKQSTARGDRDALSQVLFNLLRNAMDALG
                     GDLENLSPSQSDKWIRISTRNVDQSVVVNVEDNGPGVKSGLGAQIFEPFVTTKAQGHG
                     LGLSISRQICLDHGGELDLTVGASRNGASFDMRVPCAY"
     gene            complement(1784..3466)
                     /locus_tag="PLH57_03420"
     CDS             complement(1784..3466)
                     /locus_tag="PLH57_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01687.1"
                     /translation="MKQKFKNIAVVMMGALVSGCSFFDAPPVPNERPGGGSISVNQAI
                     EGGCQTRLGGQVAQVVTVPLKEKHDKDVWVYWTAGSDSVQEQPDSAWVESANWDLRVK
                     RKIPVIGTNGGITSRKIAKLAEGQARAALLFWSTRLRDPLPEPEILENRADWSEDRYE
                     GIEFDTLSLIPDYQNPLINRVHLSNTAETGILSAYDPGSHDLTLSGNVFILRAANGRD
                     FFALRFCEYSRPGNWIQFQWVRLAHTWNNERGEGMNLKNWSQVAGVALGLGLASVVVA
                     EEVIENGSPDVVQTEVVAEATPTPEASPTPTVAPEVEPVATAAPEAEPSPTPVVEASP
                     TPAPAVLKECTFDVAFAPVPPKPEETRVLWNGLNLFNCSVVRATRETNEVKSESGELT
                     KLPGGVFPGVLEGTEEQEELKWQVRVRQAYVGTNAAVAGFVKDAKFETLDVVAEKQTW
                     AADRLFDADGKYGPGGVDYYNPTVMGWARFYDPERHAFMPVGQVYVVKTPSGHFAKLQ
                     VVKYIVNGGGYEADPEQEGGFVWNPDQGPKDGRIHMVLRSVANLTKDDTRLK"
     gene            complement(3463..>4399)
                     /locus_tag="PLH57_03425"
     CDS             complement(3463..>4399)
                     /locus_tag="PLH57_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRK01688.1"
                     /translation="LLAGVQGELHEISSSRLPDARTVSTTRVNPYLGARRAFPLNTVE
                     SALVVGAGTRYDSNPKLWSPRAEIAHTVQSSLGTHETTLHSGLGFRDVTAKEKYLEFE
                     NSAVGYRVIGNPALQMERAWISELKHSLLAGIDRFEVGISAVRVSDAIGYVPTQTNSG
                     IWSYDNVGNTQTFGARAQWAHYFGNEFNGRLQWLGLRGRNLSTNGDLFLQPTHRVSLQ
                     FGRSVTRGFSFLGTAVWTARQAFFDANRNGLTDDEWIASFWNGALEASYGFRLTPIAD
                     VFTFFVRGDNLLDVVRPKTFPIEPRTFLAGLRAEL"
BASE COUNT         1047 a         1194 c         1039 g         1119 t
ORIGIN      
        1 attgagcttc accatcgcat cagaagcatc tgccgcgatc gtcactgttt gccccaatcg
       61 cgtgagtaca tcgccaatgg cttcccgatt gtgagcttcg tcttcaacca ctagtatgcg
      121 catggcaccc tcatatcaaa actagctccg tttcgagaag caccaacagt cagatccagc
      181 tcgccgccgt gatccaagca aatttggcga gaaatgctga gccctaagcc gtgtccttga
      241 gctttggtcg taacaaaagg ctcgaatatt tgagccccca accccgattt cacgcccggc
      301 ccattgtcct caacgttcac gaccacggac tgatccacgt tcctcgtgga aatgcgaatc
      361 catttgtcgc tttgagacgg gctaagattt tctagatctc ccccaagggc gtccattgca
      421 tttcgcaata agttaaatag cacctgcgat aatgcgtcgc gatcacctcg agcagttgat
      481 tgtttcgcgt cgagttttag ttctacccgt atctttcgtg cggccaattc ctgggccacg
      541 aattcaacac aatcctcaat caatgcgtgg agatcgactg actcatattt gcgcgcctga
      601 tcccgcacac tacccaaatc aaggtaccga tccgtaattc gctcgagacg ttgaatttgc
      661 tgggtcacgg tgtcgactcg tttgagtaaa gcgtctcctt cgccaatttt gctttctaaa
      721 tcttctttaa gcatttcaag ttggagaccc atggaattga gcggattacg cacctcgtgg
      781 gccacttgcg aagacatatt ggctgcaaga aggagcttct tggcatgagc cagttttcgt
      841 tcggtttcaa ccgcagcttc aagtttcttt ttttgttcgt taatggtttt ttcacgctcg
      901 cccagcgttg ttgccatgcg cgtgaactcg cgggtaagaa tttgcatttc ttccggcata
      961 ctcccccgca ccgggggata cttaatatct gaatgagtgc ctcgtagaac gatttcgcgt
     1021 gcgacgcgcg tccattccga aactggcact acccagcggt tgagtttgag cagaacaatg
     1081 agcgaaagca ccagcgtgcc aatcaaaaaa aacagtacga tactttgcga agtgcccaac
     1141 tcgctttcaa ttaaatgctg attttcttgg gcaaccaatt cgattcgatg gctgagatca
     1201 gaccattcgg tggtaaacac ggaagtcagc gaagctcgaa aattggaata aagcagcgcc
     1261 gcacctgtgt agtctttgtt ctttagtcgc tcggcgagac tcacaaaaaa ctcagtctct
     1321 tttttggaaa tatcgtcgac ccgtcgtaac caagtagcca cttcgtctac aaccgcaaaa
     1381 tgaacgtcgc gtttgttttt aacatttgaa ttgcttcgag cggcttggag aattttttca
     1441 aggtcggtga catcagagcc aaaggctttc gccaaaccgg accgacccga aaccgtagtg
     1501 ggttttgtgc gagctcccca acgacgaaac cccacgtttt gttcgaaggt gcgttgataa
     1561 gcctgccact taccttgcat gacattaagc gatcgaatta ctggcagata atattggttc
     1621 accgcgcttg cgcgattttc tacgcgatcg agccgccaca cgagcacgag cgttaacgca
     1681 gtcacgacta cggccatcgc gactacgctc caaaccacct ttaacgtgga tcgtttcgtt
     1741 ccccagtcca tggctctatt ctagcctgac cagcgacgag cgactacttt aaacgtgtgt
     1801 cgtctttcgt aagatttgcc accgaacgaa gaaccatgtg aattcgtccg tctttcggac
     1861 cttggtcagg gttccaaaca aatccgccct cttgctccgg atcggcctca taacccccgc
     1921 cgttcacgat gtattttact acttgcagct tagcgaagtg gccgcttgga gtttttacaa
     1981 cgtaaacttg gcccacgggc atgaacgcgt ggcgctcagg gtcgtaaaaa cgagcccagc
     2041 ccatgacagt aggattgtag tagtcgacgc cacccggacc atacttaccg tctgcgtcga
     2101 acaggcgatc agcggcccaa gtttgttttt cggcgaccac gtcgagtgtt tcaaattttg
     2161 cgtctttcac aaatccagca acggcagcat tggttcctac gtaggcttgt cgtacacgca
     2221 cctgccattt taattcttct tgttcttcgg tgccttcgag aacgccaggg aacactccgc
     2281 ctggaagttt cgttaattct ccagactcag atttaacttc gttcgtttca cgagttgccc
     2341 gaaccactga acaattgaaa agatttaatc cgttccaaag aacgcgcgtt tcttcgggtt
     2401 tcggtggtac cggcgcaaac gccacgtcaa acgtacattc tttaagaact gcaggagctg
     2461 gcgtaggcga ggcttctacg acgggtgttg gggaaggttc tgcttctggt gctgcggtcg
     2521 ccactggctc aacttcgggc gcaaccgttg gcgttggcga agcttctggg gtcggcgtgg
     2581 cttctgcgac gacttctgtt tgcactacat ctggactgcc attttcgata acttcttcag
     2641 ctactaccac tgaagccaat cctaatccga gtgcaacacc cgctacctga gaccaatttt
     2701 ttaaattcat accttcacct ctctcgttat tccatgtatg cgccaagcgc acccattgaa
     2761 actgaatcca atttccggga cggctatact cacaaaaccg cagagcgaaa aaatctcgcc
     2821 catttgccgc acggagaata aaaacgttac cgctaagcgt caaatcatga gatcccgggt
     2881 cataggccga cagtattccg gtttcagctg tgttacttag atgcactcga ttgatcagcg
     2941 gattctgata gtcaggtatc agacttaggg tgtcaaattc aataccttcg taacgatctt
     3001 ccgaccaatc tgcacgattt tcgagaattt ctggttcggg caggggatcg cggagacgag
     3061 tgctccaaaa aagtaaagcg gccctggcct ggccttcagc taacttagcg atttttctag
     3121 aggttattcc accgttagtg ccaattaccg gaatttttcg ttttacacgt aggtcccagt
     3181 ttgcgctttc cacccaggcg gagtcgggct gttcctgcac cgaatcgctc ccggcggtcc
     3241 aataaaccca aacatctttg tcatgtttct ctttaagcgg cacagtgacg acttgcgcga
     3301 cttgtccacc cagccttgtt tgacaaccac cctcgattgc ttggtttacg ctaatcgatc
     3361 ccccgccggg acgttcgttc ggaactggtg gcgcatcaaa aaaactacac cctgagacca
     3421 acgctcccat catgacaact gcgatgtttt taaatttttg tttcataact cggctctgag
     3481 tcctgccaaa aaagttcgag gttcaatcgg aaatgttttg ggtcgcacta cgtccaagag
     3541 attgtcgcca cgaacgaaaa aggtaaacac atcggcaatc ggagttaggc gaaatccata
     3601 agaagcttcg agggcaccat tccaaaatga agcgatccac tcgtcgtcgg tgagtccgtt
     3661 tcggttggca tcgaaaaagg cttggcgagc cgtccaaacg gcggtgccta aaaacgaaaa
     3721 tcctcgcgtt acactccgac cgaactgcag cgatacacgg tgtgtgggtt gcaaaaataa
     3781 atcgccgttg gtcgatagat tgcgaccgcg aagcccaagc cactgaaggc ggccgttaaa
     3841 ttcgttaccg aaataatgag cccattgagc gcgcgcgcca aacgtttgag tgttgcccac
     3901 attatcgtaa ctccaaattc cgctattggt ctgagttggc acgtacccaa ttgcatcact
     3961 cacgcggaca gccgaaatgc cgacttcgaa acgatcgatc cctgccagga gcgaatgttt
     4021 aagctctgaa atccaggctc gctccatttg aagtgcgggg ttgcctatca ctcgatatcc
     4081 gacggcggag ttttcgaatt ctaaatactt ttccttggcc gttacatcgc gaaaacccag
     4141 gccggaatga agagttgttt cgtgcgttcc tagagaagat tgcaccgtgt gcgcaatttc
     4201 ggcgcgtggg ctccagagtt tggggtttga atcataacgc gtacctgcac ccacgaccag
     4261 cgctgactcg acagtgttca gtggaaacgc gcgccgagca cctagatagg gattcacccg
     4321 cgtagtggat accgtgcgcg cgtcaggcaa tcgcgacgaa ctaatttcgt gaagttcacc
     4381 ctgtacgccc gcgagaagc
//
LOCUS       DAOMXO010000113         4378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10779_length_4378_cov_3.935924, whole genome shotgun sequence.
ACCESSION   DAOMXO010000113 DAOMXO010000000
VERSION     DAOMXO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10779_length_4378_cov_3.935924"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PLH57_03430"
     CDS             <1..919
                     /locus_tag="PLH57_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01689.1"
                     /translation="RDCRHHPRYHRGNAPHRRIWSRFPYMFDWVLQLSRGRAAETIEV
                     SAWSEMLLFLKSEKILIVVGTYICFAIGFATFTKKKLTNDGAIRFLAVYLAILACTAV
                     MLSKRPNSYYLIPVLASGLPVLCVVIWRLFSVAPETRKKDLLIPKIAFVVVTGLMFFG
                     PLVSTLQYDFRLLTQLRKDRIEHEKIPRKYNHCTLIGTYTSSLISYALAYGNSWAHEL
                     FNQDLRRLYPKFIQFRENGYSGFGNFGLPVSLDSLRKRVRDGECIFFYGSSGLGEQPP
                     KSHEKFTLKAKESSGSETLFEILEIQGKH"
     gene            1006..3180
                     /gene="ppk1"
                     /locus_tag="PLH57_03435"
     CDS             1006..3180
                     /gene="ppk1"
                     /locus_tag="PLH57_03435"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRK01690.1"
                     /translation="MFKPSARLGPLLVDPTKPYIYRDVSWLQFNQRVLAEAADKKNPL
                     FERLKFLSISASNLDEFFMIRMAYVHRSIAGQAKVAESARSGELSKPVDTNKAARLQR
                     IGSYILRGVRKFHEQQRVVLNDLARDFAKQEIRIVTDVFATRELNELGEKIFFERILP
                     DLQNPRPLQWPLLADIDNLQIFATVFPIKGNKLFVAVPKKVKSVFVERDPKTGVDYVF
                     FLDQLLEAFLRRVLNVEGAVGVFRLTRDGDVSVELHDEVNFGSIPDTVRKSLSGRDRG
                     RPIRLQYRGDVPPLLLFAIQKALRLKTEQLFQEPLSFALGGLWSATSALATQVSSNPK
                     LAHPPVDSFVPEAFNSAEVFERVKRSDYLFHHPYDSFEGYIRFIQTACTDPSVVEIAQ
                     TVYRMDAISPVIEALKMAARNKRIRVVIELRARFDEWNNLKLADELRQAGADVVFGFG
                     KLKIHSKIALVTRKEDGKEIFYTHLSTGNYHAGNAKQYTDLSIITANSDIGQDAKHFF
                     DSVALGKVPNDFKVLVPAPLKLHKKLLQMIESEVEVQNTGGKGRIFAKVNALIDETII
                     EHLYRASAAGVQIDLMVRGACSLVPGLKGLSENIRVFSIVDRFLEHSRIYYFEGAKKL
                     YLSSADWMPRNFHSRLELAFPVLSEKIFSYLADVAIPAYLADNVKSRYLSSDGIWRSR
                     VVKKSDREIRAQGVFEQQANNEYKGTNFERKNFDSSTPRAPG"
     gene            3134..3613
                     /locus_tag="PLH57_03440"
     CDS             3134..3613
                     /locus_tag="PLH57_03440"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HRK01691.1"
                     /translation="MNEKTLILVRHAHRDKDQGAGFDNGLSEKGQDQARKIGAHFATR
                     LEGSNPVFLTSPKKRCVETIQAIYKAVLKDTKNVKAPKVEELLGEGPYIDDRVEKFFD
                     WWKDEASDLVVACSHGDWIPTFVHFTVGIDIPLKKAAYVQLNLVGSRPMLTWLVQRV"
     gene            complement(3610..>4378)
                     /locus_tag="PLH57_03445"
     CDS             complement(3610..>4378)
                     /locus_tag="PLH57_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01692.1"
                     /translation="ATMSTGELISIPRMDLKRVDMILSGSILLEECAAVIGATKIVAT
                     EFSLRDGIMYEQSLGLSTATGASLLAHLDDIVAWAARFGVNEKESRKHLRNAALVFRS
                     SKKMHRLSRHWEPYLLAAAVLRRTGELISSVDFGAHSAYIVEHAKFPFLEDWETRLIA
                     HICRSSDPTGAKKFDRKQLRRLELPMECAPITGLLILCETLHLGGAPIGNFRVRGRGK
                     KWYLHHPRLTAGGVEAVKLQQYSKLFKDAFEKKIEIG"
BASE COUNT         1160 a          949 c         1072 g         1197 t
ORIGIN      
        1 gcgagattgc cggcaccacc cgcgatacca tcgaggaaac gctccacatc ggcgtatttg
       61 gagtcgcttt ccttatatgt ttgattgggt gttgcagtta tcgcgcgggc gagctgcaga
      121 aacgattgaa gttagtgcct ggtccgagat gttgcttttc ttaaaatctg aaaaaattct
      181 aatcgtcgta gggacttaca tttgttttgc aatcggcttc gctactttta ccaaaaaaaa
      241 actaaccaac gacggtgcaa ttagattctt agcagtctat ttagcaattt tagcatgcac
      301 cgcagttatg ctttcgaaac gtccaaattc ttattattta attccggtct tggcatctgg
      361 cctgccagta ctttgtgttg tgatctggcg attgttctcg gtcgcacctg aaacgagaaa
      421 aaaagatctt ctgattccaa aaattgcctt tgttgttgtg acgggattaa tgtttttcgg
      481 accgttggtt tccactctgc agtacgattt tcgcctgctc actcaattgc ggaaagaccg
      541 gatcgaacat gaaaagattc cgcgaaagta caatcattgt actttgatcg gaacttacac
      601 ctcatcatta attagctatg ctttggccta tggcaactct tgggctcacg aactgtttaa
      661 ccaagatctt cgtcgtctgt atccaaagtt cattcagttc cgggaaaatg gatacagcgg
      721 tttcggaaac tttggtttac ctgtttcgct ggactctttg cgaaaacgcg ttcgtgatgg
      781 tgagtgtatc ttcttttacg gtagttcggg actcggagaa cagccgccga agagtcacga
      841 aaaattcacg ttaaaggcca aagaatcctc ggggtccgag accctttttg aaatcttaga
      901 aattcaagga aaacattaat ttacctttgc aaataccgac agtggtcacg agcgcaaagc
      961 ccagcttgcg aattcaatta gatatagtta ctaggtgtca ctagaatgtt taaaccaagt
     1021 gccagacttg gacctctgct cgttgatccg actaaacctt acatttaccg cgatgtgagt
     1081 tggttgcagt tcaatcagcg ggtacttgcg gaagcggccg ataaaaaaaa tccgcttttt
     1141 gagcgtctta aatttctttc gatttctgct tcaaaccttg acgagttttt tatgattcgc
     1201 atggcatacg tgcaccgtag cattgccggt caggcgaaag tagctgagtc cgccaggagt
     1261 ggagagcttt cgaaaccggt ggataccaat aaagcggcgc gcctacagcg gattggtagt
     1321 tacattctgc gaggggttcg aaagtttcac gaacaacaac gcgtagtgct caatgactta
     1381 gctcgggatt ttgccaaaca agagattcgg attgtgaccg atgtgtttgc gactagggaa
     1441 ctgaatgaat taggggaaaa aatttttttt gagcgaatct tacccgatct tcagaaccct
     1501 cgcccacttc agtggccttt actagccgat atcgataatc tgcaaatttt tgcgaccgtg
     1561 tttcccataa aaggaaataa gctcttcgtc gcggtaccga agaaagtaaa gtcagttttc
     1621 gtagaacgtg accctaaaac aggggtagat tatgtatttt ttctcgacca attgcttgaa
     1681 gcgtttctgc gtcgcgtatt gaacgtcgaa ggtgctgtag gggtgtttcg gctcacgcgc
     1741 gacggtgacg taagtgtcga gttacatgac gaagtgaact ttggatcgat cccagacact
     1801 gtgcgcaaga gtcttagcgg tagggatcga ggacgtccga ttcgtttgca ataccgaggc
     1861 gatgtaccgc cacttttact tttcgcaatt cagaaggccc ttcgtttgaa aaccgagcag
     1921 ctgtttcagg agccgttgtc gtttgcatta ggtggtcttt ggtccgcgac tagtgcactc
     1981 gccacccaag taagttctaa tccaaagttg gcgcaccctc cggtggattc atttgtacca
     2041 gaagcgttta atagtgctga ggtgttcgaa cgagttaaac gatcagacta cctgtttcat
     2101 catccttacg actcattcga agggtacata cggttcatac agacagcctg cacagatccg
     2161 agcgtagtgg aaatagcaca aaccgtgtac cgcatggacg ccatttcacc ggtaatcgaa
     2221 gcgttgaaaa tggctgctcg caataaacgc atacgcgtgg tcattgaatt gcgggcacgg
     2281 tttgatgagt ggaataattt aaaacttgca gatgagttac gacaggccgg ggccgacgtg
     2341 gtgtttggtt ttggaaaatt gaaaattcac tctaagattg cgctcgtaac tcgtaaggaa
     2401 gatggaaaag aaattttcta tactcattta tctactggaa actatcatgc agggaacgcc
     2461 aaacagtata ctgatctctc aattatcaca gcaaattcag atatagggca ggatgcaaaa
     2521 catttttttg attcggtggc tctaggaaaa gttccgaatg attttaaagt tctggtaccc
     2581 gcgcccttga agcttcataa aaaattattg caaatgattg agtccgaggt tgaagtgcaa
     2641 aataccgggg gcaaagggcg tatttttgcc aaagtgaatg cgctgataga tgaaactatt
     2701 attgaacatt tgtaccgggc ttcggccgcc ggcgttcaga ttgacctaat ggtgcggggc
     2761 gcttgctcgt tggtgccagg ccttaaaggg ctcagtgaaa atattcgagt cttcagtatc
     2821 gtcgatcgct ttctcgaaca cagtcgaatc tattatttcg aaggggctaa gaagttatat
     2881 ctctccagcg ccgattggat gccgcgcaat tttcactctc gcctagagtt ggcgtttcct
     2941 gtgttgagcg agaagatttt ttcgtatctt gccgacgttg ccataccagc ttatttggcc
     3001 gataatgtga agagtcgtta tttaagctca gacggtattt ggcgttcacg agtagtaaag
     3061 aaatcggatc gtgaaattcg tgcgcaggga gtgttcgagc agcaagcaaa taacgagtac
     3121 aaaggaacta attttgaacg aaaaaacttt gattctagta cgccacgcgc accgggataa
     3181 agatcaaggt gcggggttcg ataacggtct tagcgaaaaa gggcaggatc aagcccgaaa
     3241 aattggcgcg cactttgcga cccggctcga aggaagcaat cctgtttttt tgacgtcccc
     3301 caaaaaaaga tgcgttgaaa cgattcaagc tatttacaag gcggtactca aagacaccaa
     3361 aaacgttaag gcgccgaaag tggaagagtt gttaggtgaa gggccctata tcgacgaccg
     3421 agtggaaaag ttctttgact ggtggaaaga cgaagcaagt gatcttgttg ttgcttgttc
     3481 tcacggcgat tggattccga ctttcgttca tttcaccgtc ggaatcgaca tcccgctaaa
     3541 aaaagccgcc tatgtacaac tcaatttagt gggttctagg cccatgttaa cctggctcgt
     3601 ccagcgcgtt tagccaattt cgattttctt ttcgaaggcg tctttaaata attttgaata
     3661 ttgttgaagc ttaacggcct ctacgcctcc ggcggttaat cgaggatggt gcaggtacca
     3721 tttttttcca cgaccgcgga ctcgaaaatt accaatgggg gcaccgccta aatggagcgt
     3781 ttcacaaagt atgagcaggc cggtgattgg ggcgcattcc atagggagtt caaggcgtcg
     3841 caattgtttg cgatcaaatt ttttagctcc cgtggggtca gagcttcggc aaatgtgggc
     3901 gattagtcgc gtttcccagt cttcgaggaa gggaaattta gcatgctcta caatatatgc
     3961 agagtgagcc ccaaagtcga ccgacgaaat aagttctcct gttcgacgaa gaacagctgc
     4021 cgctaacaga tatggttccc aatgtcgcga caatcgatgc atcttcttgg aactgcgaaa
     4081 aaccaatgct gcgttccgaa ggtgtttgcg agactctttt tcatttactc cgaaacgcgc
     4141 ggcccaggca acgatatcgt cgagatgtgc aagaagactg gcgccagtag cggtcgataa
     4201 acccagcgat tgttcgtaca tgatcccgtc tcgcaaagag aactctgtag ctacaatctt
     4261 agtggcgccg attacggcag cacattcttc cagtaaaatt gaacctgata aaatcatgtc
     4321 gacgcgtttc aaatccattc gagggataga aatcaattca ccagtggaca tagttgcc
//
LOCUS       DAOMXO010000114         4375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10789_length_4375_cov_5.229630, whole genome shotgun sequence.
ACCESSION   DAOMXO010000114 DAOMXO010000000
VERSION     DAOMXO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10789_length_4375_cov_5.229630"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLH57_03450"
     CDS             complement(<1..742)
                     /locus_tag="PLH57_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01693.1"
                     /translation="MRSRKKAKSKPVKRSLVKKKKTRVLARKKAVRTIAIPTHLTAFV
                     AAQDPSLYTFIDHASWRFILRVSQRFFAKKAHEKYLEGLEETGICIERIPLISEMDAK
                     LKRFGWRAVPVVGFIPPAVFLEFLSIGVLPIACDMRTLEHLGYTPAPDIVHEAAGHAP
                     ILADRSYAKYLKQYGEVSRKAIIAQHDLNLYDAIRELSDIKENPSSTSADIAKAEREL
                     QKALSLIDHTSEAAELSRMAWWTTEYGLV"
     gene            complement(793..2415)
                     /locus_tag="PLH57_03455"
     CDS             complement(793..2415)
                     /locus_tag="PLH57_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01694.1"
                     /translation="MSFVFIFRKSLTLIVGLVFAVYPFCSWAALANGTFAQIVAPASG
                     PSGAVAGLLTADLLEFDQQSFPNPSVYRHVVRHLVPGDWIRKSLSTNLNSLNPQTWWI
                     NDSYRLYTAQAQNSLAPIQPMATPVYSIAEWDLGTVVAVTDQNRLVLLDRRKLVPNSV
                     PALGNASVVDPSIYGQILDIHMVSDSQFVAVTDKLYLIKVETFGDVNRSGKVEFEVLL
                     PNTRLTFSPLPNHAIDAGVPSDVVSVVGGSSSAWVLVRPSTPLRWNGYWHSFVVRFDY
                     HSWKPQAAAAIPYPSGSKDLELLGTENAGRDLILRAGNSYEGVDSRGMRLPKSALVFA
                     MSPPPVGGPILSGQLPLLKRSGAPQILIDHPKEGHQSWVPRSAIPLHTHSKFTLKAGS
                     GTQWQITATPPTGGNGTVATVPSSVGPVTYAQPGSSSGVAAVVPQTPPNTADPKVKRD
                     FLALFLKEFPANGGNPTVKGFFSQITATYGPRYALDYLSDAECLRFYFTRTGKDTYAV
                     LPGHEKEILDLEKLSEKLEEAYERSCNGVVKS"
     gene            complement(2412..>4375)
                     /locus_tag="PLH57_03460"
     CDS             complement(2412..>4375)
                     /locus_tag="PLH57_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRK01695.1"
                     /translation="VLLLTPPMTQLNTPYPASAALYGFLKNRAGELNVQPYQRDLSIE
                     LACRIFSASGLKRVRERLKRDFESSAIESESVQFFLEAADDYVRVVDSVVAFLQGKNP
                     SLATRLAGRTLVPEGPRFLPLQDSAVGHHLLKMFGEMGVQDQAKYLGSLFLDDLADVI
                     AQGIDSRFSLARYGEKLAASQTSFAALNEALESEAPTLVDEMLDELSAEVVAELEPEL
                     ICLSAPFPGNVYGAFRVARQVKLKQPNIKLALGGGYVNTELRRCSETRVFNYFDFLVF
                     DDGARPLLNILEFLHGKRPQSKLLRTLVGNGTSVQWCNDAQEDDIPMAELGAPSYEGL
                     NLAQYISVIEMLNPMHRIWSDARWNKLTIAHGCYWKKCTFCDTSLDYIGRYQNTPSGM
                     VVDHMQALVRETGVSGFHFVDEAAPPAALRAISEEILKRDLVASWWGNLRFEKSFTSE
                     LAQLMSDAGCIAVTGGLEVATNRLLKLMDKGVTVEQVARVTKNFANAGILVHAYLMYG
                     FPSQTLQDTVDSLELVRQLFENDCLKSAYWHRFSATVHSPIGRNPERFGIKVLPKKFE
                     GFAENDLEFVDPTGVDHDVLGRALRRALYNYMHGLGLREDVRGWFGDEGIEMVPRPSV
                     ARNLVRNALRNEDTSLSGETENFGVRGVK"
BASE COUNT         1120 a         1194 c         1022 g         1039 t
ORIGIN      
        1 ctactaaacc gtattcagtt gtccaccagg ccatgcgcga aagctcggca gcttcactcg
       61 tgtgatcgat aagtgagaga gccttttgca actcacgctc tgccttcgca atgtcggccg
      121 acgtcgaact aggattttct ttaatatccg aaagttcgcg aatggcgtcg tacaagttga
      181 gatcatgctg tgcgatgatc gcctttcggg acacctcgcc gtattgttta aggtattttg
      241 cgtagcttcg atccgcaaga ataggagcgt ggcccgcggc ctcgtgtact atgtccggag
      301 ctggtgtata gcccaagtgt tcgagcgtgc gcatgtcaca ggcgatcggc aacacaccaa
      361 tcgaaagaaa ctctaaaaac acggccggcg gaataaaccc aaccacgggt accgcacgcc
      421 aaccaaagcg tttgagtttt gcatccattt cgctaatcaa cggaatccgc tcgatacaaa
      481 ttcctgtttc ctcgagcccc tctagatatt tttcatgggc cttttttgca aaaaaccgtt
      541 gagatacccg caaaataaaa cgccaactcg catgatcaat gaaagtgtaa agagacggat
      601 cttgggcggc cacaaacgcc gtaaggtgag taggaatcgc aatcgtccgc accgcttttt
      661 tgcgggcgag tacccgggtc ttttttttct taacgagcga acgctttaca ggtttcgact
      721 ttgctttttt tcgagaacgc atcgcctaca ttaaaactga gacttttccg attggcaatc
      781 gctctctcta gctcaagact tcacgacgcc attgcaactt cgctcgtaag cttcttccaa
      841 cttttcggag agcttttcta aatctaaaat ctctttttcg tgccccggaa gcactgcgta
      901 agtgtcttta ccagtgcgag taaaataaaa ccgaagacac tccgcgtccg acaaataatc
      961 taatgcgtaa cgcggtccgt aggtcgcggt gatctgcgaa aaaaaacctt tcacagtagg
     1021 gttccccccg ttggctggaa actctttgag aaaaagagct agaaaatctc gtttcacctt
     1081 agggtctgca gtattcggag gcgtttgagg aacaacggcg gctactccac tcgaactgcc
     1141 gggttgcgca taagtgactg gccccacgct cgatggaaca gtggctacgg tcccgtttcc
     1201 tcccgtaggc ggcgtcgctg tgatctgcca ctgagtaccg gaacctgcct ttaaggtaaa
     1261 tttcgagtgc gtatgcaaag gaattgcaga tctcggcacc caactttggt ggccctcttt
     1321 tgggtgatcg atcagaattt gcggcgctcc gcttctcttc aacagcggca actgccccga
     1381 caagataggt cccccaaccg gcggaggcga catcgcgaaa accaaggcac tctttggcaa
     1441 acgcattccg cgagaatcaa ctccttcgta ggagttcccc gcacgcagga tcaaatcgcg
     1501 tccggcgttt tcggttccga gtagttcgag atccttcgaa ccgctcgggt aaggaattgc
     1561 cgcagccgcc tgaggtttcc aactgtgata atcaaaccgc accacaaacg agtgccaata
     1621 cccgttccat cgcagtgggg ttgacggccg caccaaaacc caggccgaac tactgccacc
     1681 cacgaccgac actacgtcac tcggtacgcc cgcatcgata gcgtgattgg gaagcggcga
     1741 aaacgttagc cgtgtgtttg gtaacaatac ctcgaactct acttttccgg atcgattcac
     1801 atcaccaaac gtttcaactt ttatgagata aagtttgtcg gtcacggcca cgaactggga
     1861 atccgagacc atgtgaatat caagaatctg gccataaatg ctggggtcca ctacggaagc
     1921 attgcccaaa gccggcaccg agttcggtac tagtttacgc cgatcgagca acaccaagcg
     1981 attttgatcc gtaactgcca cgacggtacc tagatcccac tccgcaatcg aataaacagg
     2041 ggtcgccatt ggttggatag gtgcgagtga attttgggcc tgcgcagtgt aaagacggta
     2101 agaatcattg atccaccagg tttggggatt caacgaattc aaattggtgg aaagagattt
     2161 gcgaatccaa tctcctggta ccaggtggcg cacaacatgg cggtaaactg aaggattagg
     2221 gaacgactgt tgatcaaatt ccaaaaggtc ggcggtcaag agtcccgcga ctgccccgct
     2281 gggccctgag gccggggcga ctatttgagc gaaagtgcca ttggccaacg cagcccaact
     2341 gcaaaatgga tagaccgcga acaccaagcc gacaattaaa gttagacttt ttctaaaaat
     2401 aaaaacaaag ctcattttac tccccttacc ccaaaattct cggtctcccc gcttaaactt
     2461 gtatcttcgt tgcgtaatgc gtttcgtact aagtttctcg cgacactagg ccttggcacc
     2521 atttctatgc cttcatcgcc aaaccaaccg cgcacatctt ctcgtaatcc gagtccatgc
     2581 atgtaattgt aaagcgctcg tcgcagagct cgccccaaca cgtcgtgatc cactccggtc
     2641 ggatccacaa actctaggtc gttttccgcg aagccttcaa attttttggg cagaacttta
     2701 atgccaaaac gctcaggatt tcgaccaatc ggactatgca cagtggccga gaatcggtgc
     2761 caataagcag attttaaaca gtcgttttcg aaaagttgtc gcactagttc caaactatcg
     2821 accgtgtcct gaagggtttg agatggaaac ccgtacatca gatacgcgtg aaccaatata
     2881 ccagcgttgg caaagttttt ggtcactctc gcgacctgtt cgacggtcac gcccttatcc
     2941 atgagtttca atagccgatt ggttgccacc tcgaggcccc cagtcaccgc gatacaaccg
     3001 gcatcactca tgagttgcgc cagttcagac gtaaaacttt tctcgaatct aagatttccc
     3061 caccaactcg ccaccaagtc tcgttttagt atttcttcgg aaatagctcg cagagcggcg
     3121 ggaggagcag cctcgtctac aaagtgaaaa ccactcacgc cggtctctcg caccaaagcc
     3181 tgcatgtgat ctacaaccat accgctcgga gtgttttgat atcgcccaat atagtcgaga
     3241 ctcgtgtcgc aaaacgtgca ctttttccag taacaaccgt gggcgatcgt caatttgttc
     3301 caccgcgcgt cggaccaaat ccggtgcatg ggattgagca tttcgatgac cgaaatatac
     3361 tgcgcaagat ttaaaccctc gtaagatggg gcaccgagct cagccatggg aatgtcgtct
     3421 tcctgggcgt cattgcacca ctgaacacta gttccattcc ccaccaaagt tcttagaagt
     3481 tttgattgcg ggcgcttgcc gtgcagaaac tccaaaatgt tcagaagtgg tcgcgctcca
     3541 tcgtcaaaca ccaaaaaatc aaaataattg aaaacccgtg tttcactgca acgtcgtagt
     3601 tcggtattca cgtaaccacc gccaagagcc aacttaatgt tcggctgctt gagttttacc
     3661 tgacgcgcta ctcgaaacgc gccatacacg tttcccggaa acggtgcact caagcaaatg
     3721 agctccggtt ctagttccgc tacgacttcc gcggagagtt catcgagcat ttcgtcaacc
     3781 aacgtaggag cctcggattc taatgcttcg ttcagagcgg caaaacttgt ctgactggcc
     3841 gccagtttct caccgtatcg cgcgagacta aaccgagagt ctattccctg cgcaattacg
     3901 tccgccagat cgtccaaaaa aagtgaaccc aggtactttg cctgatcttg aacccccatt
     3961 tcaccaaaca tctttaacag gtggtgtcct acagcagagt cctgaagcgg aagaaaccga
     4021 ggcccctccg gcaccaaagt gcgtccagca agacgcgtag caagtgaagg gttttttcct
     4081 tgaagaaacg cgaccaccga atcgacaacc cgaacatagt cgtcggccgc ttctaagaaa
     4141 aactgcacac tctctgattc gatggctgac gattcgaaat ctctctttaa gcgttcgcga
     4201 actcgcttta atccactggc cgaaaaaatc ctgcaggcta attcaattga aagatctctc
     4261 tggtagggct gaacattcag ttcgccggcg cggtttttta gaaaaccata aagcgcggcg
     4321 ctcgccggat acggcgtatt caattgcgtc atcggtggtg taagaagaag gactc
//
LOCUS       DAOMXO010000115         4367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10814_length_4367_cov_4.009045, whole genome shotgun sequence.
ACCESSION   DAOMXO010000115 DAOMXO010000000
VERSION     DAOMXO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10814_length_4367_cov_4.009045"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLH57_03465"
     CDS             <1..625
                     /locus_tag="PLH57_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRK01696.1"
                     /translation="LEYAISQKRKSLTLVHKGNIMKFTEGAFRDWGYELTKREFKGKA
                     IGWDDCEGKPPVGQVLVKDSIADITLQQVLTRPEEFDVIATLNLNGDYLSDALAAQVG
                     GIGIAPGGNINYITGHAIFEATHGTAPKYANQDKVNPGSVILSGEMMFRHLGWNEAAD
                     LIIRGMDRSIADRTVTYDFARLMTAEGLSGVKEVKCSEFGDCIIKNM"
     gene            647..1636
                     /locus_tag="PLH57_03470"
     CDS             647..1636
                     /locus_tag="PLH57_03470"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRK01697.1"
                     /translation="MKAPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLELT
                     PAQNALKGVMMELEDCAFPLLAGMVGTDDPNVAFKDANVCLLVGARPRSKGMERKDLL
                     TENAKIFTVQGEAIGKNANPDVKVLVVGNPCNTNAYIAMKTAEKFGRVKTKNFTAMLR
                     LDQNRALAQLATKAKRPVGSIKKLAVWGNHSPTMYPDYRSATSNGDALKTLVNDDTWN
                     RETFIPTVGKRGAAIIEARGASSAASAANAAIDHMRDWWLGTKGEWTTMGVPSDGSYG
                     IPKDIVFGYPCTTENGEYKIVQGLEIDAFSKERIALTLKELEEERAGVADLLK"
     gene            1751..2929
                     /gene="sucC"
                     /locus_tag="PLH57_03475"
     CDS             1751..2929
                     /gene="sucC"
                     /locus_tag="PLH57_03475"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRK01698.1"
                     /translation="MNVHEYQAKELLRRYGVPLLAGKAALSVDEAVIGAEELKAKTGT
                     GVWVVKAQIHAGGRGKGGGVKVAKSIDEVRNHAQKILGMQLVTHQTGPDGKKVHKLFV
                     EQGCDIAKEYYCALLVDRATSHVILMASSEGGMDIEEVAAKTPEKIIKVDIDPAIGYQ
                     PFHGRKVALGIGVPKESMNKAASFFGALYKAFMDLDCSMCEINPLVLTKQGDLVALDA
                     KLNFDDNALFRHKDVAEMRDPTEESEAETEAAKYDLAYISLDGTIGCMVNGAGLAMAT
                     LDIIKLHGAAPANFLDVGGGATKEKVTVAFKIILKDPKVKAILVNIFGGIMKCDVIAE
                     GIIAAAKEVNLAVPLVARLEGTNVELGKKMLAESGLRITPASDLTDAAKKVVAAAGGR
                     "
     gene            2929..3801
                     /gene="sucD"
                     /locus_tag="PLH57_03480"
     CDS             2929..3801
                     /gene="sucD"
                     /locus_tag="PLH57_03480"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRK01699.1"
                     /translation="MSIWINKNTKVICQGITGSQGLFHSKGCKDYGTQLVGGVTPGKG
                     GTTVEGFAVFDSVAEAKNKTGCNVSMIFVPPPFAADAICEAVDAELDLVVAITEGIPV
                     LDMLRVKKYMQGRKTRLIGPNCPGIITPGECKIGIMPGHIHKKGPVGVLSRSGTLTYE
                     AVFQLTQAGVGQSTCVGIGGDPINGTNFIDVLDAFNRDPETKAVIMIGEIGGSAEEEA
                     AEFIKREMKKPVAGFIAGQAAPKGKRMGHAGAIISGGKGTAQEKIAALEAGGVKVARS
                     PADIANTLFSVWKP"
     gene            3856..4284
                     /gene="ndk"
                     /locus_tag="PLH57_03485"
     CDS             3856..4284
                     /gene="ndk"
                     /locus_tag="PLH57_03485"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRK01700.1"
                     /translation="MAIERTFSIIKPNAVTKNVMGEIISRFEKRGLRIAAAKFLKLSK
                     EKAEGFYIEHKDRPFFGSLVMFMTSGPVLLMALEGENAVALNREIMGATDPAKAAPGT
                     LRKDFADSIEANAVHGSDSPKSAEREIAYFFDKSEIQGRF"
BASE COUNT         1200 a          980 c         1185 g         1002 t
ORIGIN      
        1 gctcgaatat gcgatttcac aaaaacggaa aagcttaacg ctcgtgcaca aaggtaacat
       61 catgaagttt accgagggcg cgttccgcga ctggggctac gaactcacga aacgtgaatt
      121 caaaggaaag gccattggtt gggacgactg cgaaggtaaa ccaccggtcg gccaagtgct
      181 cgtgaaagat tcgatcgcgg acatcacact tcaacaagtg ctcacgcgac ctgaagaatt
      241 cgatgtaatc gccactctca acttgaatgg cgactacctc tcagacgctt tagcagctca
      301 agttggtggt atcggaattg ctccgggtgg caacattaac tacattaccg gccatgcaat
      361 ttttgaagcg actcacggca ccgcaccgaa gtacgccaat caagataaag tgaatcctgg
      421 ttcggtgatc ctttcgggcg aaatgatgtt tcgccaccta ggttggaacg aagcagcaga
      481 tttaattatt cgcggcatgg accgctcgat cgcggatcga acggttacgt acgactttgc
      541 tcgcttaatg acggcggagg gcttatctgg cgttaaagaa gtaaagtgtt ctgagttcgg
      601 tgattgcatc atcaagaata tgtagcaaca gctcctggag gacaaaatga aagcacctgt
      661 tcgagtggca gtaaccggcg ccgccggtca aattggctac gcacttttat ttcgtattgc
      721 gagtggagag atgttgggca aggaccaacc tgttattttg cagctcctag agttaacgcc
      781 agcgcagaac gcgcttaagg gcgtgatgat ggaacttgaa gactgcgcgt tcccactgct
      841 tgcgggaatg gttggtactg acgatccaaa cgtggcgttc aaagatgcta atgtgtgttt
      901 gcttgttggc gctcgcccac gttcaaaagg aatggaacga aaggatctac ttaccgaaaa
      961 cgcaaaaatt ttcaccgttc agggcgaagc gatcggtaaa aatgcgaacc ccgatgtgaa
     1021 ggttttggtg gtcggcaacc cttgtaacac gaacgcctac atcgccatga aaacggcaga
     1081 aaagtttggt cgtgtgaaaa cgaaaaattt tactgccatg ttgcggctcg atcaaaatcg
     1141 cgcgcttgcg caattggcga cgaaggctaa gcgcccggtc ggtagcatta aaaaactcgc
     1201 agtgtggggt aatcacagtc cgacaatgta tccggattac cgatcggcga cttcaaacgg
     1261 tgatgccttg aaaaccttgg ttaacgacga cacctggaat cgtgaaacct tcattccgac
     1321 tgtaggtaaa cgcggggccg ccattatcga ggctcgtggc gcatcgtcgg cagcgagtgc
     1381 cgcaaatgcc gcaatcgacc acatgcgcga ctggtggctt ggaaccaaag gcgaatggac
     1441 cacgatgggt gttccgtccg acggcagcta cggcattcct aaagatattg tatttgggta
     1501 cccgtgcact accgaaaatg gcgaatacaa aattgttcaa ggtcttgaaa ttgatgcgtt
     1561 ctccaaagag cgcatcgcgc ttacgctcaa agaattagaa gaagaacgag ccggcgtagc
     1621 tgatttgttg aaataaaaac gaagtaaatt ttttgatgac cggaaaacgg agcaccttgc
     1681 ggcgtttatt acagcgccgc gactaagaaa ggggcgtacc ttaccggctg attcgagaca
     1741 ggagtcgaga atgaacgttc atgagtacca ggccaaagaa ttgcttagac gttatggcgt
     1801 gccactgctc gctggaaagg ccgcgctcag tgtagacgaa gcggttatag gcgccgaaga
     1861 gttgaaggct aaaactggca cgggcgtgtg ggtggtgaaa gcccaaattc acgctggcgg
     1921 gcgtggtaag ggcggcggcg taaaggtggc aaaatccatt gacgaagttc gcaatcatgc
     1981 ccaaaaaata ttaggcatgc agctcgtcac tcaccagacg gggcccgacg gaaaaaaagt
     2041 tcataaactt ttcgttgaac aaggctgtga cattgcaaaa gaatattact gcgcgctttt
     2101 ggtagaccgt gcgacgagcc atgtgattct aatggcctct tctgagggcg gcatggacat
     2161 tgaagaagta gccgccaaaa ccccagaaaa aattattaaa gtcgatattg atcccgcaat
     2221 tggttaccaa ccatttcacg gtcgcaaggt cgcacttggt atcggtgttc cgaaggaaag
     2281 catgaacaaa gcggctagct tttttggagc gctttataaa gcattcatgg atcttgattg
     2341 cagcatgtgt gaaatcaacc cacttgtact taccaaacaa ggcgaccttg ttgcgctgga
     2401 tgcgaaatta aattttgacg acaacgcgtt gtttcgccac aaagacgttg ccgaaatgcg
     2461 cgaccccaca gaagaaagtg aagctgagac cgaagctgct aagtacgatc ttgcgtacat
     2521 tagtctcgac ggcacgattg gttgtatggt gaatggcgcc ggccttgcta tggcgacact
     2581 cgacatcatc aaactgcacg gcgcggcacc tgcgaacttt cttgatgtag gtggcggcgc
     2641 gaccaaagaa aaagtcactg ttgcgttcaa gattattttg aaagatccca aagtgaaggc
     2701 catcttggta aacatctttg gcggcattat gaaatgcgac gtaattgctg aaggtattat
     2761 tgctgcagca aaagaagtta atctcgcagt tccgttggtg gctcgtctcg agggcacaaa
     2821 cgtagagctt ggaaagaaaa tgttggcgga atcggggttg cgtatcactc cggcttctga
     2881 cttaaccgac gcagcaaaaa aagtagttgc agcggcagga ggacgataat gtcgatttgg
     2941 atcaataaaa acaccaaggt gatttgccaa ggcattactg gttctcaagg actttttcac
     3001 tccaaaggct gcaaagatta cggcacccaa ctcgtgggtg gagtaacgcc tggtaagggt
     3061 ggaactacgg ttgaaggttt tgcggttttc gacagcgttg ctgaagcgaa aaataaaaca
     3121 ggctgcaatg tttcgatgat ttttgtgccg cctcctttcg cggccgatgc catttgtgag
     3181 gcagtcgatg cggagttgga tttggtagtg gccatcaccg agggaattcc cgtactcgac
     3241 atgcttcgag tgaaaaaata catgcaaggc cgcaaaacgc gactcatcgg gcccaattgt
     3301 ccgggcatta ttacgccagg ggaatgcaaa ataggcatta tgccgggcca cattcacaaa
     3361 aaaggtcctg tgggggtact ctcccgcagt ggcacgctca cttatgaggc agtgtttcag
     3421 ctcacccaag cgggcgtcgg tcaaagcacc tgtgtgggga tcggcggcga ccctatcaat
     3481 ggtacgaatt ttatcgatgt actcgacgct ttcaatcgcg accctgaaac gaaagctgtg
     3541 ataatgattg gcgaaatcgg cggcagcgcc gaagaagaag ccgcggaatt cataaaacgt
     3601 gaaatgaaaa agccagtggc tggatttatt gctggccagg ctgccccgaa aggcaagcgc
     3661 atgggccatg ctggggcaat catcagtggg ggcaagggaa ccgcgcaaga aaagattgcg
     3721 gcattggagg caggcggtgt aaaagtggcc cgaagcccag ccgacattgc gaatactttg
     3781 ttttcagttt ggaaacctta acaaggcggc caacggctag atttttatag gccctgaatt
     3841 attaggagac ttaccatggc aatagagaga actttttcga tcattaaacc aaacgccgtc
     3901 accaagaacg tgatgggcga aattataagc cggtttgaaa aacgtggctt acgtatagcg
     3961 gctgctaagt ttttgaaact ttctaaagaa aaagcagaag gtttttatat cgagcacaag
     4021 gaccggccat tttttggttc cctcgttatg tttatgacca gcggaccagt gctcctcatg
     4081 gcgctcgaag gcgaaaacgc tgttgcgttg aatcgtgaaa ttatgggtgc gactgatcct
     4141 gcgaaagcgg ctccgggcac gcttcgtaaa gactttgctg atagcattga agcaaatgca
     4201 gtacacggct ccgacagtcc taagtcggct gagcgagaga ttgcttactt tttcgataaa
     4261 agcgagatcc aaggccgctt ttaactagcg ctgaagtctt gatcaaaatt gcgctttgtc
     4321 agacaaatac gatgatcggt gattttgtcc acaatgtgga taaaatc
//
LOCUS       DAOMXO010000116         4363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10830_length_4363_cov_5.538301, whole genome shotgun sequence.
ACCESSION   DAOMXO010000116 DAOMXO010000000
VERSION     DAOMXO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10830_length_4363_cov_5.538301"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="PLH57_03490"
     CDS             <1..1533
                     /locus_tag="PLH57_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01701.1"
                     /translation="PIRFLEKDDGSPVIYKMERPDSSVDYIFYIIPYWTDELDELLRG
                     EIPGFTKQRQLRPLTVTQLGIKVEQFNYMYVGDRLADEQYLNTEHMVRVTVPPSEVAA
                     FEFFLTNPPGIQVKIMFSFEAEKMDKYAEFKLTHKEVYDAITHGGAGRYKFSRAEISS
                     SLTDYISKKEFEMKSKGDIQIPDVINRAIDECFTPVPKVDTRRRRPGYPSYPSYDPGG
                     YGGGGVGIPYYLGGPVSVAPESLEQGDARIARAYLMLLEREEPKYLISGPSIPGGGVV
                     TSDDPFGPSSVIPTPPPSGPFPTNRPSPWNPGGREQTSIEYVFKKEIVNSTKVFYYSN
                     QHFIDTSVTAAVKVNLSNKPQDQVSKPRVVDIVKMTAVVEATATTDNPLKPGITVKSG
                     EQYVLQSAFSLWAESYYADYVRKPFPWNASWESPDPDLYYRVGEGPWTPVNGRALIES
                     DSLYKGELQFYIDKAKVWSKISKDYRERKYAGFKRAIFTYGRTFPQFQVTVTGRKIEL
                     RD"
     gene            complement(1552..3243)
                     /locus_tag="PLH57_03495"
     CDS             complement(1552..3243)
                     /locus_tag="PLH57_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRK01702.1"
                     /translation="MQTKKLTTLPAAKRTAIAAFGMIACLYSAACVPTPEQQGALDKA
                     LANSDEPKEVEAPDVQPSCFNERFVQPHAERTNKLDLLFVTDTSGSLDAERGKIADGI
                     DAFVLALPEHVDFQIAVMLGHGSNSNHSGAIYKHKNSKHVLKSAELSLDELRAMLKMN
                     LMNQPMDHHSDGGEIMSYSFMYGLEHGKAQSSRAKGFFRDDAALAVIFVTDENDICAE
                     YPVGVKPVPDPSGGEAASKKRDCSGVTTASLLAKLKAFQGDRPYLVGGIYYNNLKTVP
                     KGDENEFGYGIDDIVKTAHGVSVDMADGQYSQGLAEIGNLTTIKLNLYTDFTLARTNV
                     DISTLRVEVDGQNSEYSYNADSNEVHIEDAGDALSVIDINYCLKQVPSPTPSPAPSAT
                     PEPSASPAPTATPEPSASPAPSPAPTATPEPSASPAPSPAPTATPEPSASPAPSPAPT
                     ATPEPSASPVPSPTPTPGGQSDFNTFGFDAATSSNSAYLLWYTPGHASTSQVYWGTTP
                     QLGNASEVNNALTETHELTLMGLEPGTTYYFQSVSIDANGGRSYSQIISKTTKAQ"
     gene            3521..>4363
                     /locus_tag="PLH57_03500"
     CDS             3521..>4363
                     /locus_tag="PLH57_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01703.1"
                     /translation="MKTMNKSIALGLLAAVFGAQAIAAPGALQVRERTRDIVKDREVG
                     RTGRSGKDAVRANERNTNTGLTVGSTARDAKGIDASAALQKMGTTGEAVRTSAVGAAK
                     DALAVEKTPALDAQEKASVKRDITTVRRELGNEVADHLTSLEGSVKADGLLLMKGLTE
                     VPASVAPREQIKEMVNEIVAYQKATGVWPIHTQAFVAACSKELDAGSYATLLNIFRGA
                     RSGEDILANMGENNGMFGTGRISAERKLIALDGIMTNDKLEKTGATKCKIMPAGKILQ
                     TAKGV"
BASE COUNT         1139 a          962 c         1132 g         1130 t
ORIGIN      
        1 ccgatacgct ttttagaaaa agacgacgga tcgcccgtga tctacaaaat ggagcggccc
       61 gatagcagcg tcgattacat tttttacatc attccttatt ggaccgacga gttagacgaa
      121 cttttgcgag gcgaaattcc gggtttcact aagcagcgcc agcttcggcc tctgacggtc
      181 actcaactgg ggatcaaggt tgaacaattc aattacatgt acgtaggtga tcgattggcc
      241 gacgaacaat accttaacac cgaacacatg gttagagtca cggttccacc gagtgaagta
      301 gcagcttttg agtttttttt aaccaatcct cctgggattc aggtcaagat catgttctct
      361 ttcgaagccg aaaaaatgga caagtacgcc gaatttaagc ttacccataa agaggtttac
      421 gacgcgatta cccatggggg agccgggcgc tacaagtttt ctcgggccga aatttcttcg
      481 tcgttgaccg attatatttc aaaaaaagaa tttgaaatga agtctaaagg agacattcaa
      541 attccagatg ttatcaaccg tgcgatcgac gaatgtttta cgccagtgcc caaggtggac
      601 acacgacgta ggagaccggg gtacccgagt tatcccagct atgatccggg tgggtatggt
      661 ggcggcggag taggaattcc ttattacctt ggtgggccag taagcgtagc gccggaatct
      721 ctggaacaag gagacgcccg aatcgctcgc gcttatttaa tgctgttgga gcgtgaggaa
      781 ccaaaatatt taatttcggg accatccatt ccgggaggcg gagttgtaac gtcagacgac
      841 ccttttgggc cgtcttctgt gattccaact cctccgccat ccgggccatt tccaacaaat
      901 cggcctagtc catggaatcc gggcgggcgc gaacagactt caattgaata cgtatttaaa
      961 aaagaaatcg tgaattcgac taaagttttt tattattcca accagcactt catcgatacc
     1021 agtgtaaccg ctgcagtgaa ggtgaatctt tcgaataagc cacaggatca ggtctcgaaa
     1081 cctcgagtgg ttgatatcgt aaaaatgacg gctgttgttg aagccacggc tacgacggac
     1141 aatccgctga agccaggaat tacggtgaag tctggagaac agtatgttct tcagtccgcg
     1201 ttttcgttgt gggcagaaag ttattacgcg gattacgttc gaaagccgtt tccctggaat
     1261 gcgtcgtggg aaagtcccga ccctgatctt tattatcggg tgggcgaagg tccttggact
     1321 ccggtcaatg gtcgtgctct gattgagtct gactcgctct acaaagggga actacagttt
     1381 tatatagata aagctaaggt ctggagcaag atcagcaaag actatcgcga gcggaaatac
     1441 gcgggcttta agcgtgcgat attcacctac ggcagaactt ttccgcagtt ccaagttacc
     1501 gtaacgggtc gaaaaatcga actgcgagac tagccttaga tcgcagtact attattgggc
     1561 tttggttgtt ttactaatga tctgactata cgagcgacca ccgttggcgt cgatcgacac
     1621 gctttgaaag taataagtcg tgccaggttc tagtcccata agtgtaagtt catgggtttc
     1681 agtgagcgcg ttatttactt cagacgcgtt tcctaactgc ggagttgtgc cccaatacac
     1741 ttggctcgta gaagcgtgac ccggcgtgta ccaaagcagg tacgcggagt tgcttgacgt
     1801 cgctgcgtcg aatccaaaag tattgaaatc agattgtccg cccggtgtcg gagtaggact
     1861 aggaactggc gaagcgctag gctctggagt tgcggttgga gcgggcgaag gagcaggcga
     1921 agcgctaggc tctggagttg cggttggagc aggcgaaggt gctggcgaag cactgggctc
     1981 tggagttgcg gttggagcag gcgaaggtgc tggcgaagca ctaggctctg gagttgcggt
     2041 tggagctggc gaagcactag gctctggggt tgcgcttgga gcaggcgacg gtgtaggcga
     2101 cggcacttgt tttaggcagt aatttatatc aatcactgaa agtgcgtcac ccgcatcttc
     2161 aatatgtact tcgttagaat cggcgttgta actatattct gagttttgcc cgtcgacttc
     2221 tacgcgaaga gtagaaatgt cgacatttgt tcgtgcaagc gtgaagtcgg tgtagaggtt
     2281 cagtttaatc gtcgtaaggt tgccgatttc tgctaatccc tgcgagtatt gaccgtctgc
     2341 catatcaaca cttacgccgt gggcagtttt aactatatcg tcgataccgt agccgaattc
     2401 attttcatca cccttaggaa cggtctttag attgttgtag taaatgccgc ctaccaaata
     2461 tggacggtcg ccttgaaaag cttttaattt tgcaaggagg ctagccgttg tcacgccgct
     2521 gcagtcgcgt tttttagaag cagcttcacc gccgcttggg tctggaactg gttttacgcc
     2581 tacaggatat tcggcgcaga tatcgttttc atcagtgaca aaaatcacag ctagggccgc
     2641 atcatcacga aaaaatccct ttgctctgct cgactgtgct ttaccgtgtt ccagtccgta
     2701 catgaaagag taactcatga tttcaccacc atcactgtgg tgatccattg gttggttcat
     2761 gaggttcatc tttagcattg ctctcaattc atcgagtgag agttcggcag atttcaaaac
     2821 gtgtttacta tttttatgtt tgtaaattgc tccactgtga ttggaattac ttccgtggcc
     2881 caacatcacg gcaatttgaa agtctacatg ttccggaaga gctaatacga acgcatcgat
     2941 gccatcagca atttttccgc gttcagcatc gagagacccc gatgtgtcag tcacaaataa
     3001 tagatctaat ttattcgtac gctctgcgtg tggttgaaca aatcgttcgt tgaagcagga
     3061 aggttgtaca tcgggtgctt cgacttcttt tggttcgtcg gaattggcca acgccttatc
     3121 gagcgcgcct tgttgttcag gtgtcggtac acaggcggca ctatatagac atgcgatcat
     3181 accaaacgca gcgattgcag ttctcttggc agcgggaaga gtggtgagtt ttttggtctg
     3241 cactgggcac cgtccttctt gaatttacta aaatcatttt acgctttgtg ttaagaaaaa
     3301 caaacaattt cagtattgta tttaagtgcc tgatttttat gaaaaaaaaa ttaccagtgt
     3361 caaagattta gtcattttcg gagaagctca ctggattaga agagaagggg aaatgaattc
     3421 tgaacaaaaa tagtcaagat acatgcttga cggcggtcct tcttccatgt aaaagcgggt
     3481 caaacaaatt tcgcataaca gcgaaggagg gaaacaagaa atgaaaacta tgaataaaag
     3541 tattgcatta ggtttgttgg cagctgtgtt tggggcacaa gctattgctg ctccaggcgc
     3601 gcttcaagtg cgtgaaagaa cacgcgacat cgtgaaagat cgtgaagtag gtcgaactgg
     3661 tcgtagcggc aaagacgctg tacgcgccaa cgagcgaaac acgaacacag gtttgactgt
     3721 aggttcgaca gctcgcgatg ctaaaggcat cgacgcatcg gctgctctcc aaaaaatggg
     3781 gaccactggt gaagctgttc gcacttcggc agtaggcgct gcgaaagacg cgcttgcagt
     3841 tgaaaaaact ccagctctcg atgctcaaga aaaagcttcg gtaaaacgcg acatcaccac
     3901 agttcgccga gaactcggta acgaagttgc ggatcacctg acttctcttg aaggttcggt
     3961 aaaagctgac ggcttgctct tgatgaaggg cttgacggaa gttccagctt cggttgctcc
     4021 acgtgagcaa atcaaagaaa tggtgaacga aatcgtagct taccaaaaag cgacgggcgt
     4081 atggccaatt cacactcaag cgtttgttgc agcttgcagc aaagaacttg atgctggttc
     4141 ctacgctact cttctgaaca ttttccgtgg cgcacgtagc ggtgaagaca ttctcgctaa
     4201 catgggcgaa aacaacggta tgtttggaac tggccgcatc agcgctgagc gaaaactcat
     4261 tgctcttgat ggaatcatga ccaacgacaa gcttgaaaaa acgggcgcta caaaatgtaa
     4321 aatcatgcct gctggcaaga ttttgcaaac ggctaagggt gta
//
LOCUS       DAOMXO010000117         4358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10845_length_4358_cov_5.021148, whole genome shotgun sequence.
ACCESSION   DAOMXO010000117 DAOMXO010000000
VERSION     DAOMXO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10845_length_4358_cov_5.021148"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..2381)
                     /locus_tag="PLH57_03505"
     CDS             complement(147..2381)
                     /locus_tag="PLH57_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01704.1"
                     /translation="MTRILRLVPLLFLGILTSEIAGAVSLEPVGHATDEEVGSTRYIY
                     FPVIGAAADNCNNSRTYPSSLTPIDPTTGEVLFYVTATSSLSEIYTGSTGTTVNSGDL
                     SIRVELVSSNTPVALNDQGAGYNDGVTGHEVNYGNTENARKLIGLSLPDNGAGSYGLC
                     GLPAIWGATTNCRPGLAANQPSITLRIGVIREGQAFSDTDDEDAEDVTIRLVDCPPHA
                     SGALIEPTLDFDLRPGDERLTITNRTSAPSDPFGISQVVAYGTKSSTTVTLSNSLPAG
                     GGFQRAIGTSASAISIDGLENDTQYCVSLGYVNKGGLVATDSTWSANLPDNLDSSRAC
                     VTPSRIDGFLQKNGCFIATAAFGDEWHPRVNTLREFRDRILLKTALGTSFVNWYYSWS
                     PKKAEWLKSRPWARAAVRTALLPLVTGASAVLWLKEHLTYTWQRLKKLEFGVKSAKAE
                     TIQPYIDSIKKKYNLPDPETESIAEVAEPIAVPQATPYPTGNTEAVQPYIDELQSKAR
                     AEESSKSASTPSSQGVQPYIESVKKGKELEPKYRGSVNRAAGLSILTSSDFDVDSNSI
                     QANQFNSVYETKSKYVPGVDLYYEHQLLRDRTFGALGLVSHLKVFVARGRGIFTRRQV
                     RSDDTKFRFTAFPVSLGVGYRFLLARFIVPYVHVSGVMTPMIESRDDQKPTRKALSRG
                     TSVIVGGSLNLDWITRKDAWSRYDAYDVLHTYLTLQWEKARSFSGAVDFNYDAVYAGL
                     GFEF"
     gene            complement(2378..3157)
                     /gene="pssA"
                     /locus_tag="PLH57_03510"
     CDS             complement(2378..3157)
                     /gene="pssA"
                     /locus_tag="PLH57_03510"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRK01705.1"
                     /translation="MQDSQEVQRDARPRRRVYLLPNLFTTANLLCGFYAVIAAFNGNF
                     INAAWSIIIAILFDSMDGRVARLTRATSNFGVQYDSLSDLTSFGIAPAFVGYLWCLQP
                     YGRLGWLAAFVYVVCAALRLARFNVSADTIPKKYFQGLPSPVAAATVASAILFYQDME
                     FAFHRPPFLLALMLTQASFMISSIRFSSFKEFKMNRGNSFGVLAVVILTIVLIAIRPE
                     VTLFGMCLLYTLANVSIHLYSLTGANKKVAAEFEAKGTLPK"
     gene            complement(3160..3402)
                     /locus_tag="PLH57_03515"
     CDS             complement(3160..3402)
                     /locus_tag="PLH57_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRK01706.1"
                     /translation="MADHEVRSRAKVAFGQIAGFVARRIVCYVEKGTPLSRGERYGMI
                     RFGSRMEVMLPLNSEILVNVGDRTTAGETKLARFGE"
     gene            complement(3351..3872)
                     /locus_tag="PLH57_03520"
     CDS             complement(3351..3872)
                     /locus_tag="PLH57_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRK01707.1"
                     /translation="MASILKNHRLPVQIEGFPYIFGALLLTLSSAMIWKAAVSGTSDV
                     GAGARWAAATGTFVFGVFTAFCIWFYRNPNRKTPTDDPRAVISPADGRVLSVTEVSGN
                     RIGAEKARKVSIFMSPMDVHVNRAPITGVVEGVQYNSGKFFKADLDKASEENENNWLI
                     MKSDRGQKLLLAK"
     gene            complement(3909..>4358)
                     /locus_tag="PLH57_03525"
     CDS             complement(3909..>4358)
                     /locus_tag="PLH57_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01708.1"
                     /translation="AQALGKPMQGVSSLKALAYSYSANSSQKVIPIVNAYQKSVFVGA
                     EENGDWVDRSLAPEAVGSWAQGQFAGKSYIFVGNGALVYAAELRGSFPEGTVLELENS
                     EIEAQGLAKAVSEAMTKTPRGVSHLDVHANYVRPSAAEAKLDKSDQN"
BASE COUNT         1132 a         1142 c         1010 g         1074 t
ORIGIN      
        1 gaagaaaacc ccgaaactcg aacgttcgcg atctttgaaa gagtctgctc aagtgtactc
       61 tgaactgtag tttgaccaat tcgctggcgc tgaaaaccgt gataagcagt tccgtcgtag
      121 gaaagtgtta ttctaaatcg cttcatttaa aattcgaagc caaggcccgc gtagacggca
      181 tcgtaattga aatcgacagc gccactaaag cttcgagctt tttcccactg gagcgtcaaa
      241 taggtatgta atacgtcata ggcgtcgtac ctagaccagg cgtctttgcg agtgatccaa
      301 tcgagattca aggacccacc aacgatcacg ctagtgcctc tggaaagtgc cttacgtgtg
      361 ggtttttgat cgtcccgtga ttcaatcatt ggcgtcatta ctccggaaac atgcacatac
      421 ggaacaatga accgcgccaa aagaaaacgg tagccaacac ctaagctcac gggaaaggca
      481 gtaaaacgaa actttgtatc gtcggaccgc acttgacgac gagtaaatat accccgacca
      541 cgagccacaa aaactttaag atgacttacc aaccctaatg cgccaaatgt acgatcgcgc
      601 agtagttggt gttcgtagta taaatcgaca cctggaacat atttgctttt ggtttcgtac
      661 accgaattaa attgattagc ctgaatggag tttgaatcaa cgtcgaaatc gctcgaagtt
      721 aagatcgaca accccgcggc tcgattaacc gacccacgat attttggctc aagttctttt
      781 ccttttttaa cgctctctat ataaggttga acaccctgac tgctcggggt tgacgcgctt
      841 ttgctggatt cctcggcccg tgccttgctt tgaagttcgt cgatataagg ctgaacggct
      901 tcggtgttac ctgttggata gggtgtcgct tgcggtaccg ctatcggttc cgccacttcg
      961 gctatcgatt ccgtttcggg atcgggtaaa ttatattttt ttttaatact gtcgatgtag
     1021 ggctgaatgg tttcggcctt ggcagattta actccaaact ccaacttctt tagtcgctgc
     1081 cacgtgtagg tcaaatgctc ttttaaccat agcaccgcgg acgcaccagt aacgagcggc
     1141 agtagtgccg tacgcacggc agctcgcgcc caaggtctgc tttttaacca ctcggccttc
     1201 tttggactcc aactatagta ccaatttacg aaactcgtac cgagggctgt tttcaacaaa
     1261 attcggtctc gaaactcacg caaggtgttc acacgagggt gccactcgtc accgaaagca
     1321 gcggttgcga taaaacagcc atttttctgt aaaaatccgt cgattcttga aggagttacg
     1381 catgcccgcg aactatcaag attgtccggt aagttggcgc tccaagtgga gtcggtagcc
     1441 actaatccgc ccttgttcac ataacccaga ctcacgcagt attgagtgtc gttttccaga
     1501 ccatcaatcg aaattgccga ggcagaagta ccaattgccc tttgaaaacc gccccccgcg
     1561 ggtagcgagt tcgaaagcgt cactgtagta gagctttttg taccgtaggc taccacctgc
     1621 gaaatcccaa atggatcact cggcgcagaa gtgcgatttg tgatcgtcaa cctctcgtca
     1681 cctggtcgca agtcgaagtc gagcgtgggt tcaattaacg cgcccgaggc atgcggcgga
     1741 caatccacta agcgtattgt cacatcttcc gcgtcttcgt cgtctgtgtc cgagaacgct
     1801 tgaccttcgc gaataacccc tattcgaagt gtgatcgatg gttgattcgc agcgaggccg
     1861 ggtcgacagt tggtggtcgc gccccaaata gcgggcagac cgcaaagtcc gtacgaacct
     1921 gcgccgttat caggcaagct aagcccaatt aacttacgcg cattctcagt attgccgtag
     1981 ttgacttcat gccccgtcac tccgtcgttg tagcctgcgc cctgatcatt taaggcaacc
     2041 ggagtgtttg acgaaaccaa ttccacccga atggaaagat ccccggaatt tacggtggtt
     2101 cccgtggagc ctgtgtaaat ttcactgagc gagctggtcg cggtcacgta aaataaaact
     2161 tctccggttg tcggatcaat cggggttaga ctcgaagggt aggtgcgaga attattgcag
     2221 ttgtccgcgg ccgctccgat gactggaaaa taaatatacc tggtgcttcc aacttcttcg
     2281 tcagtggcat gccccactgg ttcgagcgaa acagcacctg caatttcgct cgttaaaatc
     2341 cccaaaaata gtagtggtac taaacgcaag atccgtgtca cttcggcagg gtccctttcg
     2401 cttcaaactc cgcagctact tttttatttg cgccagtcaa cgaatacagg tgaatgctta
     2461 cattggccaa cgtgtagagc aaacacattc cgaaaagtgt cacttcgggg cgaatcgcaa
     2521 ttaaaactat ggttaagatc actaccgcta agactccgaa cgagtttccg cgattcattt
     2581 taaattcttt aaatgacgaa aatcgaatgc tgctaatcat aaacgatgct tgagtcaaca
     2641 tgagcgccag caaaaagggg ggccgatgaa acgcgaactc catgtcttgg tagaacaata
     2701 ttgcactagc taccgtagcc gctgccacgg gactcggaag accctgaaag tacttttttg
     2761 gaatcgtatc ggccgacaca ttaaatcgtg caagtctaag cgcggcacaa accacgtaaa
     2821 caaatgcggc caaccagccc agccttccat agggctgcaa acaccacaga taaccaacaa
     2881 acgctggagc gattccgaat gaagtcagat ccgaaagcga atcgtactgt accccaaaat
     2941 tacttgtcgc gcgggtcagg cgtgcaactc gcccatccat ggaatcaaaa aggattgcga
     3001 taataataga ccaagccgca ttgatgaagt tcccgttaaa ggctgcgatt actgcgtaaa
     3061 aaccgcaaag taagttcgca gtggtaaaga gatttggaag caaataaact cgtcgtcgcg
     3121 gtcgagcatc tctttgaact tcctgtgaat cttgcattct tattccccga agcgtgctag
     3181 ctttgtttcc cctgcagttg tgcggtctcc cacgttcacc aatatttccg aattcaaagg
     3241 aagcattact tccattcgag acccgaatcg aatcattccg tagcgttcac ctcgcgaaag
     3301 tggagtgcct ttttcgacat aacaaacaat tctacgtgca acaaaacctg ctatttggcc
     3361 aaaagcaact tttgcccgcg atcggacttc atgatcagcc aattgttttc gttttcttcg
     3421 gaagccttat cgagatcggc cttaaaaaat ttcccagagt tgtactgaac gccctcgacg
     3481 actccagtaa ttggcgctcg attgacgtga acgtccatgg gcgacataaa gatactgact
     3541 tttcttgcct tctcagcccc aatgcgattt ccggaaactt cggttacgga aagcacacga
     3601 ccgtcggccg gcgaaatgac ggcgcgaggg tcatcggttg gggtttttcg attgggattc
     3661 cgataaaacc aaatacaaaa agcagtgaaa accccgaata cgaacgtacc cgtcgcagct
     3721 gcccaacgag caccagcgcc cacatcgcta gtaccactta cggcggcctt ccaaatcatg
     3781 gcgctcgaga gcgtgagtaa taacgcccca aaaatataag gaaatccttc gatctgaacc
     3841 ggaagtcggt ggttttttag tattgaagcc atgacagtta cctcgctcga tttgattgcg
     3901 agaacttatc aattttgatc agacttatca agcttcgcct cggcggccga aggtcgaacg
     3961 tagttggcgt gaacatcaag atgactgacc ccacgcggcg ttttggtcat ggcctcgcta
     4021 actgcttttg cgagtccctg cgcttcaatc tccgaattct cgagctctag cactgttcct
     4081 tcgggaaaac ttccgcgcaa ctcggccgcg taaaccaacg ctccgtttcc aacaaagatg
     4141 taactttttc cagcaaactg accttgagcc cacgacccca cagcctctgg cgccagagaa
     4201 cgatcgaccc aatcgccgtt ttcttcggcg ccgacaaata cacttttttg gtaggcgttt
     4261 acgatcggaa tgactttctg cgacgagttc gcactatacg aatacgcgag cgctttaagc
     4321 gaagaaaccc cttgcatggg ttttccaaga gcctgcgc
//
LOCUS       DAOMXO010000118         4352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10859_length_4352_cov_4.409123, whole genome shotgun sequence.
ACCESSION   DAOMXO010000118 DAOMXO010000000
VERSION     DAOMXO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10859_length_4352_cov_4.409123"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3635
                     /locus_tag="PLH57_03530"
     CDS             <1..3635
                     /locus_tag="PLH57_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01709.1"
                     /translation="EAFAALNQPISCFSGTKTISNGAMHVLGKIAKYAPERAAYSTAR
                     EIAILTKVGADACEFPANIDSSMDAVFKLVSDDQHLHGATFARLLQAMRAADARPIHS
                     QSVLDLGLSTSAQERYLRLLVELLADQGGESSFLQAVVPLFTELAQPARNVIANTFYL
                     SYGIAPQSKDREVLVAILTSLMENREPLRVRDMIVRTVGATKARESVYDVFVDGFLKL
                     SPADFADAFVKAANAISKSDGKPFVRVLAENARKGLLLNDVQPATDILLDVFENADRH
                     SSLVQTVYEVADLPEFADAVELTARMAQDGSLKNLARGIFNIFDGRLKRVAGTPVLAK
                     RIAPLEAISKYRNLSRKSGTQIIRSRVAAVEKSFWPTSDKKMKACQNLNPAVGLLDYS
                     HAEWREELVELAVCANADGQLAAVEDFVELATDPTKPVKNGQSLAEFITRLTSQFKER
                     LSVSEKKALIAVLGEQLDHSGKRARLNELLETLPYYLRSRWCANDSGKCARSDEITFI
                     EAIADLGQAVRDHGVDLKPFTELAARLVGKNYFREALDAAVDAWIDARDGGISVSSKE
                     KPALNAQLVAEIEEKLVDAQKGWLGKNPIPSDYVGPGPAEVYQVANRYWKQVVSDFPH
                     TRYASKKDFFDRVAPMIDSLADPKFGLLEQAATFFYLMDRNPYDAVWWDGWFKRLSSN
                     RTAIHYYYPGTYPGKDNPTVRVINQLDALELVVIEGDFSLKEIGQTTFNAGPIPVGEI
                     ALGLLEDIIIDPNENFAIRNLAQLARSGDKIDEWLARLKYELGVFWSFITDQNAKCSG
                     LLIDLSGIRCGELFVDELKRRLFNLRQIVPVLEALTLQQEWVSGPRGKVLRNDVGFLR
                     DVFYSVLMATPKSQQYTYRREHNTLNFIVNLVKSGTLRNIGTSMWSVNSQGEGVSTNL
                     TPVISMLVKVLVFKENGQYRLNANTIEILQTLLRKDGCWVSDPACGSRKLSERFPLLA
                     KIIETGYEWEDEDRRFTKALAYALVEILEKADRASAKNGEFFAVRLSQALRPWLGEVD
                     GVNFLARHWSDSEDLLVDRRLGSFLARVQKHKHYAELISLVSDATTEAGENGAAIAQH
                     TKSVVLTLDGDAKKRGARLANVLAAMSDDTGLNKLQRKTVEPAVQAAVDWLLEEQTIG
                     TKFQTHLSHRLKSGDLEALLDFGAKHKSKLVSVCSQFGERDVVRRIQDFTDEMGELLQ
                     NPGGRR"
     gene            3681..>4352
                     /locus_tag="PLH57_03535"
     CDS             3681..>4352
                     /locus_tag="PLH57_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01710.1"
                     /translation="MEWFARAVLPLILLCPCLAVASPATCDRGFSEERLNEKEKKALK
                     EGELVLRFQNVNDYAMGYAMQDVELDPELVMGVYISANEHSKKLGSFIKKSKILTEKM
                     PVPVFRVAYQQDVKYLSDADYVVENTVTEREKLYLLQTSLVSADDASFSPKWLDAFFR
                     VERLDDGRARITACNYQVPRSGMQKGTFNSEARSRFKRVAENLEKWVYDVANDSNKEK
                     AKFYRD"
BASE COUNT         1135 a          970 c         1155 g         1092 t
ORIGIN      
        1 acgaagcgtt cgcagcgctc aatcagccga tttcttgctt cagcggcact aaaacgattt
       61 caaacggggc aatgcacgtc ctaggaaaaa ttgcgaaata tgcacccgag cgcgccgcct
      121 actcgacggc gagggaaatt gccattctta ccaaagtcgg cgccgatgcg tgtgagtttc
      181 cggccaatat cgatagttcg atggatgcgg tatttaaact ggtaagcgac gaccagcact
      241 tgcacggcgc aacgtttgcg cgacttctcc aggccatgcg cgccgcagat gcgcgaccaa
      301 ttcatagtca gtcagtttta gatctaggat tatcgacctc tgcccaggaa cgttatctgc
      361 gcttactggt agagttatta gcggatcagg gcggggagtc gtctttctta caagcggtag
      421 taccgctgtt taccgaattg gcgcagcccg cgcgtaacgt gattgcgaac actttttatt
      481 tgtcttacgg aatcgctcct caaagtaaag accgagaagt gctcgtagcg atcttaacta
      541 gtctaatgga aaatcgtgaa ccgcttcgag tgcgcgatat gatcgtgcgc acggtcgggg
      601 ccactaaggc acgcgagtcg gtttacgacg tatttgtaga tggatttcta aaactatctc
      661 ccgcggattt cgccgacgcc ttcgtaaagg ccgcaaacgc catttcaaaa agtgacggta
      721 agccgttcgt gcgagttttg gcagagaacg ctcgcaaagg tcttttgctc aacgacgttc
      781 aacctgcgac cgatattttg ctcgatgtat ttgaaaacgc agatcggcat tcttctttgg
      841 tacaaacggt ctacgaagtg gctgatctac cagaatttgc cgacgcggta gagttgactg
      901 cacggatggc gcaagatggg agtcttaaaa acctagcccg aggaattttt aatatttttg
      961 acggacgcct gaagcgagtc gcgggtactc ctgtgctcgc aaaacgtatt gcgcctcttg
     1021 aggctatttc gaaatatcga aatcttagcc gaaaatctgg aactcaaatt atccgaagcc
     1081 gggtcgcagc ggttgaaaag tccttttggc cgactagtga caaaaaaatg aaggcttgcc
     1141 agaaccttaa cccagccgtc gggttgctcg attattcgca tgccgagtgg cgcgaagagt
     1201 tggttgagtt ggcagtttgt gcgaacgctg acggtcagct cgcagcagta gaggatttcg
     1261 ttgaacttgc gaccgaccct acaaagcctg taaaaaatgg gcaaagtctc gcggaattta
     1321 taacgcgttt aacatcccaa tttaaagagc gactgtcggt gtccgagaaa aaagcgctga
     1381 tagcggtgtt gggagagcag ctagatcatt ctggaaaacg ggctcgttta aatgaactat
     1441 tagaaactct gccttattat ctgcggagtc gttggtgcgc caatgattcg ggaaaatgcg
     1501 cacgtagcga tgaaatcacc tttattgaag cgattgccga tttgggccaa gcagttaggg
     1561 accacggggt cgacctgaag ccctttactg aattggccgc acgccttgta gggaagaatt
     1621 attttcgaga ggcactcgac gcagcagttg atgcatggat cgatgctcgc gatggtggaa
     1681 ttagtgttag ttcgaaagag aagccggcgc taaatgctca attggttgct gaaatcgaag
     1741 agaaactagt tgatgcgcag aaaggttggc ttggaaagaa tccgattcct agtgattacg
     1801 tcggaccagg tcccgctgaa gtctaccaag tggccaatag atattggaaa caggttgtgt
     1861 cggactttcc ccatacacgt tacgcttcaa aaaaggattt ctttgatcga gtcgcgccca
     1921 tgatcgactc cttggccgat cctaaatttg ggttactcga acaagcggcc acattcttct
     1981 atttgatgga ccgaaatcct tacgacgcgg tttggtggga cggatggttt aaacggctat
     2041 cttcgaatcg tacggcaatt cattattact accctggaac ctaccccggg aaagacaatc
     2101 cgacggtaag agtgatcaat cagctcgatg cactcgaact agtggtgatc gagggggact
     2161 tttcgctcaa agaaattggg caaactacat ttaatgcagg gccgattcca gttggcgaga
     2221 ttgctcttgg cctgctcgag gacattatta tcgatcccaa cgaaaacttt gcaatccgca
     2281 acctcgctca attggcgcgg tcgggagaca agattgacga gtggttggcg cgattgaagt
     2341 atgagctcgg tgtgttttgg tcttttatta ctgatcaaaa cgcgaagtgc agtgggctct
     2401 tgattgattt gtctggaatt cgctgcgggg aactgttcgt cgacgaacta aaaagacgac
     2461 ttttcaactt gcgtcaaatt gtgcctgtgc tcgaagcttt gacacttcaa caggaatggg
     2521 tttccggacc gcgcggaaaa gtattgcgaa acgacgtcgg gtttttgaga gacgtatttt
     2581 attctgtctt gatggcgact ccgaagtctc agcagtacac ctaccgacgg gaacacaata
     2641 ctttaaattt catagtcaac ctagtgaaga gcggaacgtt gcgaaacatt ggcacgtcga
     2701 tgtggtcggt aaattcccag ggcgaaggcg tgagtactaa tcttactccc gtgatttcga
     2761 tgttggtcaa agttctcgtc ttcaaggaaa acggacaata tcgactcaac gccaatacca
     2821 ttgaaatttt gcagaccttg ttgcgtaaag atggttgttg ggtctccgat ccggcttgtg
     2881 gcagtcggaa gctgagtgaa cggtttcctc tattagcgaa gatcattgaa actggttatg
     2941 aatgggaaga cgaggatcgg cgttttacca aagctctggc ctatgcgctc gtggaaatcc
     3001 tggaaaaagc cgatcgggcg agcgccaaaa acggcgagtt cttcgcggtc cgcctatctc
     3061 aggcgttgcg gccttggctc ggcgaagtcg atggagtaaa ctttctcgct cgtcattggt
     3121 ccgactcaga agatttactt gttgatcgaa ggttgggatc ttttttagct cgcgttcaga
     3181 aacataaaca ctatgccgaa ttaatatcgt tagtttctga cgctacgacc gaagcgggag
     3241 aaaatggtgc ggcgattgct caacacacta aatccgttgt gttaacgctc gatggcgatg
     3301 ccaaaaaacg aggtgcacga ctcgcaaatg tgcttgccgc catgtcagac gacaccggtt
     3361 taaataagct tcaacgtaaa accgtcgagc cagccgtgca ggccgccgtg gattggctgc
     3421 ttgaagagca gaccattggc acgaaattcc aaactcattt gagtcatcgc ttaaagtcgg
     3481 gcgacctcga ggcgcttctg gatttcggtg cgaaacataa gtccaaattg gtgtcggtgt
     3541 gttcccaatt cggcgaacgc gatgttgtgc gtcgcattca agattttacc gatgaaatgg
     3601 gtgaactctt gcagaacccc ggcggtcgac ggtaggattc agcagtaaaa ataattttca
     3661 cagaagaaat ggagacaacc atggaatggt ttgccagagc ggtattaccg cttattttgt
     3721 tgtgcccctg cctagcagtc gcttctcccg ctacgtgtga tcggggattc tctgaagaac
     3781 ggctaaacga gaaagaaaaa aaagcactaa aagaaggtga gttggtttta cgttttcaaa
     3841 acgtaaacga ctacgcaatg ggctatgcga tgcaggatgt ggagcttgac ccggaattgg
     3901 tgatgggagt ttatatctcg gccaacgaac actcaaagaa gttgggaagt tttatcaaga
     3961 agagtaaaat tctcaccgaa aaaatgccag ttcctgtttt tcgggtggcg taccagcagg
     4021 atgttaaata tttgtccgac gcagattacg tggtcgagaa tacagttacc gagagagaga
     4081 aactttatct cctgcaaaca agtcttgttt cggccgacga cgcatcgttt tctccgaagt
     4141 ggctcgacgc cttttttcgt gtggaacgtt tggacgacgg gcgagcaaga attactgcgt
     4201 gcaactacca ggtgccccgc tccggtatgc aaaaaggtac attcaatagt gaagctcgca
     4261 gtcgatttaa acgagtcgcc gaaaatctag agaaatgggt ttacgatgtt gcgaacgatt
     4321 cgaataaaga gaaagcaaaa ttctatcgcg at
//
LOCUS       DAOMXO010000119         4351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10863_length_4351_cov_4.807728, whole genome shotgun sequence.
ACCESSION   DAOMXO010000119 DAOMXO010000000
VERSION     DAOMXO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10863_length_4351_cov_4.807728"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /gene="secF"
                     /locus_tag="PLH57_03540"
     CDS             complement(<1..740)
                     /gene="secF"
                     /locus_tag="PLH57_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRK01711.1"
                     /translation="MLRIVPKNTNIDFMGKRYIWVAISTIAVLGSILLVFTKGLNYGI
                     DFTGGAEVQLAFQNSVSVGELRELMDQGGFKSTRIQRLGGDTSKEFLVKFQGEESDLQ
                     QVGSRVSAALSAKIAPTSFEVLRVDVVGPQAGGELRKSGFLSMFYALLCILIYVAIRF
                     DVRYAPGAVVALFHDSIITIGIFVLLQKEFSLQIVAAILAVIGYSNNDTIIVYDRIRE
                     TLRNYPGRPLLENVNRSINETLSRTILTS"
     gene            complement(751..2460)
                     /gene="secD"
                     /locus_tag="PLH57_03545"
     CDS             complement(751..2460)
                     /gene="secD"
                     /locus_tag="PLH57_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRK01712.1"
                     /translation="MTRNWWMKLALLVVVAVGSAIYVAPTALNVNPETTKLFYKKKVN
                     LGLDLQGGVYMVLGVDFKKIYSDVVSRLAENVQDDLKEKNIACSLPQNLETKASDDPT
                     FKVTCTSADAKKAFYALVKESHDTLRFTYDEGLIYELGISKLYRDNVREKTISQSIEV
                     IRNRIDEFGVAEPVIASQGTDRLTVELPGIKDVERAKELIGRTAKLEFKIVNDGMSPD
                     EVAAMVSELEAKNGASYKEGAKFSDYVKQINELAKGKLPTNSEIAFEREEGLTAGMGG
                     RVPYVLHSKVEVTGDDLKDAFIQVDSQTNSPEVALRFNAKGAQSFEQVTGENIGKRLA
                     IVLDGIVHSAPVIQGRIGGGEARVTLGQGDYNQKLNEAKDLSIVLRAGALPAQLEFLE
                     QRVVGPSLGADSIKKGAYAGVMGCLAIFIFMLLYYRMSGLIADFSLVLNVLIVMAVLI
                     ALDATLTLPGIAGIALTVGIAVDSNVVIYERIREELALGKDPRVAVNMGFDKAFTTIL
                     DANVTNAFAAAVLMNYGTGPVKGFAVTLLIGILATLFTAVFACKMIFEWYLNRFEVKE
                     LSI"
     gene            complement(2491..2874)
                     /gene="yajC"
                     /locus_tag="PLH57_03550"
     CDS             complement(2491..2874)
                     /gene="yajC"
                     /locus_tag="PLH57_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRK01713.1"
                     /translation="MTTLFSLAYADSVPPVPGGANTTTASTQVASGAPGAPGAPSPLA
                     SILPLVLMIGVVYFLMIRPQQKKMKEHQSLLEKIQHGDEVVTTGGVFGKVTGVADKVL
                     TLEISDGVRIKLLKNQVASVNPKLS"
     gene            complement(2871..3983)
                     /gene="tgt"
                     /locus_tag="PLH57_03555"
     CDS             complement(2871..3983)
                     /gene="tgt"
                     /locus_tag="PLH57_03555"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRK01714.1"
                     /translation="MLLKAAELKESFEPPFYPRCFTLETSHGPVHGPQFMPVGTQGTV
                     KALTPRDLKASHAEMILGNTYHLYLRPGHERIERLGGLHEFMRWDGPILTDSGGFQVF
                     SLSALNKVTDNGVEFQSHLDGSRHFFTPEHSMAIQRALGSDVVMAFDECPPFTEDKAT
                     VAKSMERTLAWARRCRDFKLKPHQKLFGIVQGGMFTDLREECLEKLVAIGFDGYALGG
                     LSIGEPVQVMHEMVKIMGPKMPQDKARYLMGVGRPEDLIVGVAYGIDMFDCVMPTRNA
                     RNGQLFTSEGKVNIKNAKYVEDTGPLDPQCDCYTCTTFTRAYLRHLFVCGEHLSGILN
                     SIHNVHFYLSLMRRLRDAIAQNRFDEIALPLYTRSL"
     gene            complement(3985..>4351)
                     /locus_tag="PLH57_03560"
     CDS             complement(3985..>4351)
                     /locus_tag="PLH57_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01715.1"
                     /translation="WRQAERVGHANFWSRFVDSGKRGLENFELASERSIDSAMLDFAK
                     ALFFDHTGLAPEEDFKTINLRDGTWQKLGTASLKNGLNKGRVRSFTYYEAKGQNRPVD
                     IDITTEFNLPYFMVIKYKP"
BASE COUNT         1137 a         1126 c          963 g         1125 t
ORIGIN      
        1 gatgtcaaaa tggttcgact caatgtttca ttgatagaac ggttcacgtt ttcaagaagc
       61 ggtcgccccg gataatttct cagggtttct cgaatgcggt cataaacgat aatagtgtcg
      121 ttattcgaat aaccaatgac cgcaagaatc gccgcaacga tttggagcga aaactctttt
      181 tgaagcagca cgaatattcc aattgtaata atactatcgt gaaacaaggc aacgaccgcg
      241 cctggcgcgt accggacatc aaacctaatc gcgacgtaaa tcagaataca aagaagagcg
      301 tagaacatag atagaaaacc gctttttcga agttctcctc cagcctgcgg ccccacaacg
      361 tccacccgga gaacctcaaa gctagttggc gctattttcg ctgacaacgc cgccgacact
      421 cttgaaccga cctgttgcaa gtccgattct tcaccttgga atttgacgag aaactctttc
      481 gaagtatcgc cgccgagacg ttgaatgcgg gtacttttaa atccgccctg gtccatgagt
      541 tcacgcaact caccaacact taccgaattt tgaaatgcca gttgaacttc agcgccacca
      601 gtaaaatcga tgccgtaatt caaacctttc gtgaaaacca acaagatact cccgagcact
      661 gcgattgtag aaatcgccac ccaaatgtac cgtttaccca tgaagtcgat attcgtattt
      721 tttggaacaa ttcgcagcat agggccctcg ttagatcgaa agttctttta cttcgaagcg
      781 atttaagtac cactcgaaaa tcatcttaca cgcaaacacc gcagtgaaca gtgtagccaa
      841 aataccgatg agcaatgtaa ccgcaaagcc ttttacggga ccagtgccgt aattcatcag
      901 cacggcggct gcaaaagcgt ttgtaacgtt ggcatcgaga attgtggtga aagctttatc
      961 aaaacccata ttgactgcca cacgcggatc tttgccgagg gcgagttcct ctcgaatccg
     1021 ttcgtaaatc acaacgttgc tatccactgc gattcccacc gttaaggcga taccagctat
     1081 acccggaagt gtgagtgtag catctagcgc gatcaaaacg gccatcacta tgagtacgtt
     1141 aagtacaagt gaaaaatccg caatcaatcc gctcatgcga taataaagaa gcatgaaaat
     1201 aaagatcgcc aaacacccca tcaccccggc gtaagcgccc ttcttaatgg agtccgctcc
     1261 gaggctcggt cccactacgc gttgttcaag aaactctagt tgcgccggca atgcgccagc
     1321 ccgtagaacg attgataggt ctttcgcttc attgagtttc tggttgtaat cgccttgccc
     1381 caatgtcaca cgggcctcgc caccaccaat tcgcccttga atcacgggcg cggaatgaac
     1441 aatgccgtca agcacgatcg ctaggcgttt gccaatgttc tctccagtaa cttgctcgaa
     1501 actctgagca cctttggcgt taaaccgaag tgcaacctct ggcgaatttg tttgagagtc
     1561 tacttgaatg aaggcatctt tgagatcatc gcctgtcact tctactttag aatgtaaaac
     1621 ataaggaact ctgccaccca ttccggcggt cagcccctct tctctctcga atgcaatctc
     1681 ggaattagtt ggcaacttgc ctttagccag ttcgttgatt tgctttacgt aatctgaaaa
     1741 cttggcgccc tctttatagg atgctccgtt tttagcctcg agctccgaga ccattgctgc
     1801 cacctcgtcg ggcgacatcc catcgttcac aattttgaac tctagttttg ctgtgcgacc
     1861 aattagttcc ttggcacgtt caacgtcttt aattcccggc aactcaaccg ttagacgatc
     1921 ggtgccttgg ctcgcaatga ccggctcagc gacgccgaat tcgtcgatcc gatttcgaat
     1981 aacttcaata ctttgactga ttgttttttc gcgtacgtta tcgcggtaaa gtttagaaat
     2041 ccctaactcg taaatcaaac cttcgtcgta ggtgaatctc agcgtatcgt gactttcttt
     2101 gactaacgca taaaaagctt ttttcgcgtc agcgcttgta caggtcacct taaaagtggg
     2161 gtcgtcgcta gccttcgttt caaggttttg cggcaacgaa cacgcaatgt tcttttcttt
     2221 caaatcgtcc tgtacgtttt cagccaatcg gctaacaaca tccgaataga tttttttgaa
     2281 atcaacacca aggaccatgt aaacgccgcc ctggaggtcg agaccaagat taaccttctt
     2341 tttatagaac aacttcgtgg tctcggggtt cacgttgagg gctgttgggg caacataaat
     2401 agccgacccc acggccacaa caacaagaag cgcgagcttc atccaccaat tgcgcgtcat
     2461 actgaatctc cctattcggt gatttgtcta ctagctgagt tttgggttga ccgaagcgac
     2521 ttgatttttc aaaagcttaa tccgaacgcc atcggaaatt tcgagcgtca aaactttatc
     2581 agcaacaccg gtaactttgc cgaaaactcc accggttgta accacctcgt cgccgtgttg
     2641 aattttttca agcaacgact gatgctcttt catcttcttt tgctgaggac gaatcatcaa
     2701 aaaataaacc acaccgatca tcaatacgag tggcagtatc gacgctagtg gacttggtgc
     2761 accgggagca cctggggccc ccgaggccac ctgagtggat gcggtagttg tattcgcgcc
     2821 acctggaaca ggaggcacac tgtctgcata ggccaatgaa aataatgttg tcataatgat
     2881 cgggtataga gtggaagggc tatttcgtca aaccgattct gggcgattgc gtcacgtaag
     2941 cgtcgcataa gcgacagata gaagtgcacg ttgtggatgg aattgagaat tccactcaag
     3001 tgttcgccac acacaaaaag atgccgtaag taggcccttg tgaaggttgt acaggtatag
     3061 cagtcacatt ggggatcgag gggcccagtg tcttctacgt atttcgcatt tttgatgttc
     3121 acctttcctt cactggtgaa taattggccg ttgcgggcat tgcgggtagg cataacgcag
     3181 tcaaacatat caataccgta tgcgactccc acaataaggt cctctggccg cccaaccccc
     3241 attaaatacc tagctttgtc ttgcggcatt ttgggtccca taattttaac catctcatgc
     3301 atgacctgaa caggctctcc gattgaaagg ccacccaacg cgtaaccatc aaacccgatt
     3361 gctaccaatt tctctaaaca ctcttcacgc aagtcggtaa acatcccacc ctgaacgatt
     3421 ccaaacagtt tttggtgggg tttgagctta aagtcgcgac atcgacgcgc ccatgccaat
     3481 gtgcgttcca tactttttgc gacggtcgct ttgtcttcgg taaaaggggg acattcatcg
     3541 aaagccatca ctacatcact cccaagggcg cgttgaattg ccatggaatg ttccggtgta
     3601 aaaaagtggc gggacccgtc gagatggctt tgaaattcta caccgttgtc ggtcacttta
     3661 ttgagtgcgg acagactaaa tacctgaaac ccaccactat cggttagaat cgggccgtcc
     3721 caccgcataa actcgtgaag cccccctaaa cgttcgatgc gttcgtgtcc tgggcgcaag
     3781 tataaatgat aagtgtttcc aagaatcatt tcggcgtgcg aagccttgag atcccgcggg
     3841 gtcagggcct taaccgtacc ttgagttccc accggcatga actgtgggcc gtgaaccggc
     3901 ccatgactcg tctcgagagt aaagcaacgc gggtaaaacg gcggttcgaa agattccttg
     3961 agttcggcag cctttaaaag catgtcaagg cttgtacttg atcaccatga aatagggcaa
     4021 attaaattca gtggtaatat caatatctac cggacgattt tgtccctttg cttcatagta
     4081 ggtaaacgat cgaacgcggc ccttattcag accgtttttt agagatgctg tgccaagctt
     4141 ttgccaggtt ccatctctaa gattgatggt tttgaagtct tcttctggcg ctagaccagt
     4201 gtgatcaaaa aacaaagctt tggcgaaatc gagcatggca ctgtcaatgc tcctttcact
     4261 ggcgagttcg aagttttcta acccgcgctt tccgctatca acaaagcggc tccagaaatt
     4321 cgcatgccca actcgctcag cttgtcgcca c
//
LOCUS       DAOMXO010000120         4336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_10912_length_4336_cov_5.884373, whole genome shotgun sequence.
ACCESSION   DAOMXO010000120 DAOMXO010000000
VERSION     DAOMXO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10912_length_4336_cov_5.884373"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLH57_03565"
     CDS             <1..604
                     /locus_tag="PLH57_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRK01716.1"
                     /translation="MTRLTCLTFLGAPRFDAHHTHPHESPNVMVVPLLILAGLSMVGG
                     FIGIPGHSWLAHWLTPVVGPHSAAHDSMEYILMAVSVLIAAAGCGLAYFIFVARPEIA
                     AKSAKQLAAIRKVLSNKYYVDEAYDGLFVGPIKKLSVALWKHFDVKVIDGLVLGVARV
                     SKAAGEIARIAHTGELQLYAVAVVFGLIAILGYMIYGINL"
     gene            588..2099
                     /locus_tag="PLH57_03570"
     CDS             588..2099
                     /locus_tag="PLH57_03570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRK01717.1"
                     /translation="MELISSSIVSLTVFLPLIFGLVLLAMPADTEAGKKLLKTAALAF
                     SLVVFLVSLGLYGRFDASYVGFQFVESIPWIESFGISYSVGVDGISLWLIILTTFLTP
                     IVILGSWTAVEDKLKTYYFLILALETGMLGAFVALDVILFYVFWEAMLIPMYLLIGVW
                     GGKDRIYASMKFFLYTLVGSLLMLIAIFYVMSQYKAQFGNYSSAILELYKVKTAFDGF
                     WSPQTLLFLAFALAFAIKVPLFPLHTWLPDAHVQAPTAGSVILAGVLLKLGGYGFIRF
                     AFPMFPQAVQYTAPVFIALGSIAIVYGAWVAMIQPDIKKLVAYSSVSHMGYVMIGLFS
                     LNHLAVAGSVYQMLNHGISTGGLFLIVGMIYERRHTREISEFGGITRIMPIFAVIFMI
                     VTLSSVALPGTNGFVGEFLILLGVYQVHKVWAIVAGTGVIFGAVYMLWMFQRVMFGPI
                     RNEENKNLKDLSLREIIVMAPIVVAIFVMGVFPNIFISKMDTSIKAFLAQINV"
     gene            2106..3602
                     /locus_tag="PLH57_03575"
     CDS             2106..3602
                     /locus_tag="PLH57_03575"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRK01718.1"
                     /translation="MEEFIKPQNLSLSPQQLSVLMPYLVVMTGGLITLLLGVFRKAVN
                     MTVPVAAFSVLTMVLAAYSLFLVWGEPTVSLFNDMMVVDNFSNLFNLIFIVATGLVIV
                     GSNQYLVREGIEHFEYYSLMLFSCLGMMFMASAKDLIVLFIALEIMSIAVYVLVGFKR
                     ADTRSNEAALKYFILGGLASAILLYGVALLYGATGSMNIQALAAAVQAGAASNLLFVL
                     GAALVTGGLLFKVATVPFHMWMPDVYQGAPTTVTAFMTTGLKAAAFAAFVRFFMNLGY
                     GADLSSTLYSGLYKVLWVSAIATMLIGNIIALTQKNIKRMLAYSSIAHTGYLIVAILC
                     GGRSEYGYSSVLVYMAGYVVMNIGAFAVVAMLSEKNDRHTNIEDYAGLGFKKPLTGLA
                     MTIFMFSMAGVPPTVGFAGKYLIFLSAVQAGEVLLAVLSVLCSAISAYYYLRVIVFMY
                     MKDPVPGRAMNHTLAPAFVIGISAVITMQLGLFPSVLIEAAKKAAMMF"
     gene            3603..>4336
                     /locus_tag="PLH57_03580"
     CDS             3603..>4336
                     /locus_tag="PLH57_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01719.1"
                     /translation="MDTRKYKVRLRSGRVLGPMGLDRICLFILRDKITGLEEARSYPK
                     GDWKDINKFPEIARMLLDKLEGRLEAPKVTAPPEELDNEIRIAPTEDLPDSVEPPGPP
                     KTDASFHIEIKTHTATPMAESSVPRTKSEIEERTVVMTPNDEKTVVQPRNELDEKQSS
                     KVNQLVEVEAVPQETPFGQSVANEPTQMIQITPTTAGPTTYAKIELEGKSEQTEGLSL
                     QGLLANKRKLSFILIAGFLVLFILFAP"
BASE COUNT         1001 a          938 c         1076 g         1321 t
ORIGIN      
        1 catgacgcgc cttacttgtc tgactttttt gggcgcaccg cggttcgatg ctcatcacac
       61 ccatccgcac gaatccccca acgttatggt ggttcctctt ttgattttgg cagggttgtc
      121 gatggtcgga gggttcattg gaattcctgg tcactcttgg cttgcgcact ggttgacccc
      181 ggtcgttggg ccgcattcgg cagctcacga ctctatggaa tatattttaa tggcggtttc
      241 ggttcttatt gcagccgcgg gttgtggctt agcgtacttt atttttgtcg cccggcctga
      301 aatagctgcc aaaagtgcga aacaattggc cgctattcgt aaagtcttgt cgaataaata
      361 ctacgtcgac gaagcttacg acgggctttt tgttggtcca ataaaaaaac tttcagtggc
      421 gctctggaag catttcgatg tcaaagtcat cgacggtctg gtgctcggcg tagctcgagt
      481 gtcaaaagct gccggcgaaa tagctcgtat tgcgcacacg ggagagttgc aactttatgc
      541 agttgcggtt gtttttggtc tcatcgccat cttggggtac atgatctatg gaattaatct
      601 ctagttcgat cgtttcgctg acggtttttc tgccgttgat tttcggcctt gttctcctgg
      661 cgatgccggc agataccgag gcaggaaaaa agcttcttaa aacagcggcg ctggcttttt
      721 cgttggtggt gtttctagtc tcgttagggc tctatgggcg atttgacgca agttacgtgg
      781 gcttccagtt tgtagagtcc attccgtgga tcgagtcttt tggaatatct tacagcgtcg
      841 gggtcgacgg aattagtctt tggctaatta tacttacgac ttttttaacc ccaattgtaa
      901 ttctcggttc ttggacggcc gttgaagaca agttgaagac ctactacttc ctaattctgg
      961 cgcttgaaac cggaatgttg ggtgctttcg tcgcattaga cgtgattctc ttctacgttt
     1021 tttgggaagc aatgttgatt ccgatgtatt tgttaatcgg tgtctggggt ggaaaagatc
     1081 gtatttatgc ttcgatgaag ttctttttat acacgttggt cgggtctcta ttaatgttga
     1141 tcgcgatttt ctacgtgatg agccagtata aggcgcagtt tggcaattat agctctgcga
     1201 tattagagct ctataaagtt aaaaccgcat tcgacggatt ctggagccct caaacgcttc
     1261 tgtttcttgc gtttgcactc gcatttgcca ttaaggttcc gttgtttcct ttgcatacct
     1321 ggctgccaga cgctcacgtt caggcgccca ctgcaggtag cgttattttg gctggcgtct
     1381 tgttaaagct cgggggatac ggatttatcc gctttgcgtt tcctatgttt ccccaggcag
     1441 ttcagtatac cgcgccagtg tttatagctc tcggttcaat agccattgtt tatggcgctt
     1501 gggttgcgat gattcaaccc gacattaaga aattggtcgc gtattcgtcg gtaagtcaca
     1561 tgggttacgt gatgataggt cttttttcgt taaaccatct ggcggtcgct gggtcagttt
     1621 atcaaatgtt gaatcacggc atatctactg gtggattatt tttaattgtc gggatgattt
     1681 acgagcgtcg tcacactcgt gaaatatcgg agtttggcgg catcacccgt atcatgccaa
     1741 tttttgctgt aatattcatg atcgttaccc tgtctagcgt ggcgctgcct ggcacaaacg
     1801 gttttgttgg agagtttctg attttgctcg gcgtgtatca agttcataaa gtttgggcaa
     1861 ttgtagcagg aaccggcgtg attttcggcg ctgtttacat gctatggatg tttcaacgtg
     1921 taatgttcgg gccgattcga aacgaagaaa acaaaaattt aaaagatctt tcgctgcgcg
     1981 aaataatcgt aatggcacca atcgtagttg ccattttcgt tatgggagtg tttccgaaca
     2041 ttttcatttc aaaaatggac acctcaatta aggccttttt ggcccaaatc aacgtgtgag
     2101 gccctatgga agaatttatt aagccgcaaa atttgagtct tagtccgcaa cagttatcgg
     2161 tactgatgcc ctatctggtt gtgatgacgg gcggactgat cacgctttta ctcggcgttt
     2221 ttcgaaaggc ggtcaatatg accgtgcccg ttgccgcgtt ctcggtgctt acgatggtgc
     2281 ttgcggccta ttctttattt ttggtttggg gtgagccgac tgtatctctt tttaatgaca
     2341 tgatggtggt agacaatttt tcgaacctgt ttaatttaat ttttattgtt gctaccggtt
     2401 tagtcatcgt ggggtcaaat caatatttag tgcgcgaggg aattgagcat tttgagtact
     2461 attctctaat gcttttttcc tgtttaggca tgatgtttat ggcctcagca aaagatctaa
     2521 ttgtgctttt tatcgcgctc gaaatcatgt cgattgcagt gtacgtattg gttggtttta
     2581 agcgggcgga cactcgatca aacgaagcag ccttaaaata tttcatattg ggtggcctcg
     2641 cctccgcaat tttgctctac ggagtagcgc ttctttatgg cgctaccggc agtatgaata
     2701 ttcaggcatt ggctgcagcc gttcaggctg gagccgcaag taatctctta ttcgttctcg
     2761 gcgcggcact cgtaacaggc gggttacttt ttaaagttgc cactgtacca tttcacatgt
     2821 ggatgcccga tgtttaccag ggggcgccca ctacagtcac cgcgtttatg acaaccggac
     2881 taaaggcagc ggcatttgcg gcgtttgtac gattttttat gaatctcgga tatggcgcag
     2941 acttgtccag cacactctat agtggccttt ataaggttct atgggtcagc gcgattgcca
     3001 ctatgctgat tggtaacata attgccttaa cccagaaaaa catcaaacgg atgttggcat
     3061 attcgtcgat tgcgcacaca ggttacctta ttgtggcgat attgtgtggt gggcgttccg
     3121 aatacggcta ttcgtcggta ttggtttata tggcaggtta tgtagtaatg aatattgggg
     3181 cttttgccgt cgttgcaatg ttgtccgaaa agaacgaccg acacaccaat atcgaagact
     3241 atgcgggtct cggcttcaaa aaacctctga ccggtcttgc tatgaccata ttcatgtttt
     3301 cgatggcggg agttccgcca actgttgggt ttgcaggaaa atatttaatc tttttgtcgg
     3361 cagttcaagc tggcgaggtg ttgctggccg ttttgtcagt gctttgcagc gcaatttcag
     3421 cctactatta cctgagagtg attgtattca tgtatatgaa agatcctgtg ccgggtcggg
     3481 ctatgaacca tacccttgcg cccgcctttg tcataggaat atcggccgta attacaatgc
     3541 aattgggatt gtttccctcg gttcttatcg aagctgctaa aaaagctgct atgatgttct
     3601 aggtggacac cagaaagtat aaagtccgat tacgaagtgg ccgcgtactg ggtcccatgg
     3661 gcctcgaccg aatttgtctt tttatattgc gcgacaaaat caccggccta gaagaagcgc
     3721 gtagttaccc aaagggtgat tggaaagaca ttaacaaatt tcccgaaatt gcgcgcatgc
     3781 ttctcgataa gctcgaaggg cgactcgaag cgccaaaagt gacggcgcca ccagaagagc
     3841 tcgataacga aatacgaatt gcacccacgg aagatcttcc ggattcagtg gaaccaccag
     3901 gaccgccgaa aaccgatgct tcgtttcata ttgaaataaa aacacacacc gctacgccaa
     3961 tggccgaatc ttctgtgccg cgtaccaaga gtgaaatcga agaacgtacg gttgtaatga
     4021 cgcccaacga tgaaaaaacg gtcgtacagc cacgtaatga attagatgaa aaacaatcgt
     4081 cgaaagttaa tcaattagtt gaagtagaag ccgttcccca ggaaactccg tttgggcaat
     4141 cggtagcgaa tgagcccacc caaatgattc agataacccc tacaacggcg ggccccacga
     4201 cctatgcaaa aattgaatta gaaggaaagt cagagcaaac cgaaggacta agccttcaag
     4261 gtctactagc gaacaagcgc aagttatcgt tcattttgat tgctggattt ttagttcttt
     4321 tcattctttt tgcccc
//
LOCUS       DAOMXO010000121         4310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11001_length_4310_cov_4.407051, whole genome shotgun sequence.
ACCESSION   DAOMXO010000121 DAOMXO010000000
VERSION     DAOMXO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11001_length_4310_cov_4.407051"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="PLH57_03585"
     CDS             complement(<1..1918)
                     /locus_tag="PLH57_03585"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRK01720.1"
                     /translation="MPVLRLTRDGVEIKTVDLSDLGKSDIVIGRAENADVRVDDRAVG
                     RSHALLKLGAKGVAIQKTSKFGRLTLNGKDIAEAIIREGDVVHLADYTVHITASVLHS
                     EHPAAMPELGTSASPITTGAAPKESKKDIDLTTTGASDNFAIGLESPTQTSSTDSGAS
                     NNKESGLELGPIDAAGMANADESIQSGAIEVETKLLEVPVTAGGEATRTHSLVGNRSS
                     EETGSLSQSSQSGSGASGDIGIDDRTAVVAPAKVMAELQFKSGDANVESFEIKRNEIS
                     LGRGSTCDVVLADKKASRKHAVIKRAGAAFVIRDLGSANGTYVAGELIAEKELQGGDL
                     VRIGDTEFIFKAVSKEYEDQKANLLSVPSVEQLPDSPEIERPQPSFSQASNLPTDNGG
                     AVFLQGADALAAQAELMGKASDAPVITGGPKARESLLAKFRRQNPVRRVSILIGLAML
                     FYLGLELDKEPGKGRSPASKVENEAGNAAFNALPPEKKQFILNTYDFAFDLYTQEKYE
                     EALYEIRKVLQILPNGYKQSKDVEAYALKAIDQIAEKAERKRKQEAEEKVRAEVASLV
                     TQAEEAVAADKIGEARELFAQILERDPENPTVARLKDEIEQKEEQKRVAEAARREKLA
                     KKETFQALLDEGQKL"
     gene            complement(1922..3001)
                     /locus_tag="PLH57_03590"
     CDS             complement(1922..3001)
                     /locus_tag="PLH57_03590"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRK01721.1"
                     /translation="MSKKVLRFKNMTVARQQGEIARLRVVGGPDTGVVFVLLVNRVSI
                     GRGEENDIVLTDIKASRTHAELILTASGVAMLHDAGSSNGLVINGVHQFKGQLRTGDK
                     IGIGLTVLEFIGAEKGGTQVLTQPPSATAMAVGTGSSGLTQFVQRTSQAVREVTKDFT
                     KSPPLLDSWNNAGSKPGSSTGGARVSIIEKNKRLVMILAALTVVATLMPEVEKKQREK
                     NREYNDPFANEASRGLASLPPPVIDKEAKKQADIFFKEGFREYREGNYLRAQVAFETA
                     LQIYTDHHLARQYLDTVKKNMAEEGKERLKIGKREQEANRFVAAYSYYDSVRRMYFRD
                     QSNPLFKEAEENINELEKKRKEWDR"
     gene            complement(3006..>4310)
                     /locus_tag="PLH57_03595"
     CDS             complement(3006..>4310)
                     /locus_tag="PLH57_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01722.1"
                     /translation="ALVPSNMLPADQLPLSGTPVSGGASVNSSSGATPAKTSLLEKLK
                     FNFEKHVINFLYNLNERHEWRSIVLTIFGFSILFSISISVFLVVSRTEQKLQSEARGR
                     AGLLARQIVDRNTSFILERMETKTDVTFAEKERGVYAAYLIDIEGRVLAPGRKLNQYI
                     TEDRFQASFAAAARKAFQDPERNRMYFEGDGRIIAVAEPVRVMNPAQGKNVTIALGMV
                     FFDQRLVTFDSGTEWLMFFYSAILAAIFAVAAFASIYRLTLRPIQHLNEEVDSVLKGN
                     SHVVTRKFKMSEMDSLIDVINAGLQRIGGGMAQPLGGNEGVSGELALTVLKVICEKVT
                     NSGVMVFSQDKRIISLNAFMEELTGIRGDSAMGTEIGNVARESAFAAFVEDLLDRTQP
                     GSAGVVQENFEVSGAAHRFECHGLGFEGTPSKGFVLIAEREG"
BASE COUNT          977 a         1162 c          941 g         1230 t
ORIGIN      
        1 gtaacttttg gccctcgtct aataaggcct gaaacgtttc ttttttagct agtttttcgc
       61 gacgcgcggc ttcggcaacc cgtttttgct cttctttttg ctcaatttcg tcttttaagc
      121 gtgcgacggt cggattttca ggatctcgtt ccagaatttg tgcaaacaat tcgcgagcct
      181 ctccaatttt atctgctgcc actgcctctt cagcttgagt tacgagtgaa gctacttcgg
      241 ctcggacttt ttcttctgcc tcttgttttc gttttcgttc cgctttttcc gcgatttgat
      301 cgatagcctt tagggcataa gcttctacgt ctttactttg tttatacccg ttaggaagta
      361 tctgtaagac cttacgtatt tcgtaaaggg cttcctcgta tttttcctgg gtgtacaaat
      421 caaaggcgaa atcataggta ttcaatataa attgtttctt ttctggcgga agcgcattga
      481 aggccgcatt acccgcttca ttctctacct tcgaggcagg gcttcgcccc ttaccaggct
      541 ccttgtctaa ttcaaggccg agataaaaca gcattgccaa cccaattaaa atgctcaccc
      601 gccgaaccgg gttctgccga cgaaacttag caagtaaact ttcccgggct ttgggcccgc
      661 ccgtgatcac tggggcatcc gaggctttac ccataagttc ggcttgggcc gcaagagcgt
      721 cagctccttg caaaaatact gcgccaccgt tgtctgttgg caaattcgac gcctgggaaa
      781 acgaaggttg gggtcgttca atttcgggtg agtccggtag ctgttcgacc gagggcacgg
      841 aaagtaagtt tgccttttga tcttcgtatt ccttgctaac ggccttaaaa ataaattcgg
      901 tatcaccaat gcgaactaga tcgccaccct gcaactcttt ttcggcgatt agctcgccag
      961 caacataggt gccattggcc gaccccaagt cgcgaattac aaaggctgcg ccagcgcgtt
     1021 ttatcactgc atgtttacgg ctagcttttt tgtccgccaa cactacgtca cacgtagagc
     1081 ctcgtcccag cgagatttca tttcgcttga tttcgaaact ttctacattg gcgtcaccgg
     1141 acttaaactg taattccgcc attactttgg ccggcgcaac caccgccgtg cgatcgtcga
     1201 ttcctatatc gccgctagcg ccagaacctg actggctact ttgggacagc gatcctgttt
     1261 cctcacttga tcgattgcct accaacgaat gggttcgagt ggcctcacca ccggccgtta
     1321 ccggtacttc tagtaatttt gtttcaactt caattgcgcc agactgaatc gactcatcag
     1381 cattggccat tccggctgca tctatcggac cgagctctag tcccgattct ttattattcg
     1441 acgccccact gtcggtcgag gaagtctgag ttgggctttc aagaccaatt gcaaaattat
     1501 cactggcacc cgtggtagtc aggtcgatat ctttctttga ttctttcggt gccgcgcccg
     1561 tggttattgg gctggcactc gttcctagct ctggcatagc cgccgggtgt tctgagtgaa
     1621 gaacactggc tgtaatatgt acggtgtagt cggctaagtg gactacgtct ccttcgcgaa
     1681 taattgcttc tgcgatatct ttaccgttga gcgtaagacg gccaaatttg ctggtctttt
     1741 gaatcgctac gccttttgct cctaatttta gtaacgcgtg actacgacct acggctcggt
     1801 catctactcg cacgtccgcg ttttcggcgc gtccgataac gatgtcagac ttacctagat
     1861 ccgataggtc gacggttttg atttcaactc cgtcacgagt taagcgtaaa acaggcataa
     1921 tttagcgatc ccactccttt cttttttttt cgagctcgtt gatattctct tccgcctctt
     1981 taaaaagcgg attactttga tcgcgaaaat acatacgacg cacactatcg taataggaat
     2041 acgcagcgac aaaccgattg gcctcttgtt cgcgtttacc tattttcaat cgctctttcc
     2101 cctcttcagc catgtttttt tttaccgtat ctaaatactg tcgcgccaaa tgatgatcgg
     2161 tataaatctg aagcgcagtc tcaaaagcta cttgagcacg caggtagttg ccttcccggt
     2221 attcgcgaaa gccctcttta aagaagatat cggcttgttt ttttgcttct ttgtcgatta
     2281 ccggtggcgg cagagatgct aatccgcgac ttgcttcatt cgcaaacgga tcgttgtatt
     2341 ctcgattttt ttcgcgttgt tttttttcta cctctggcat caaagtagct acaacggtaa
     2401 gcgccgccaa aatcataacc agccgtttat ttttttcaat tatcgaaacg cgagctcctc
     2461 ctgtactgct gccaggctta ctgcctgcat tgttccagga gtccaataat gggggggact
     2521 tcgtaaaatc tttggttact tctcgtaccg cctgagaggt tcgctgaaca aactgcgtga
     2581 gtccgctgct ccctgtgcca actgccattg cggtagcact cggcggctga gttaagactt
     2641 gagttccacc tttttccgcg ccaataaatt caagcactgt taggccaatt ccaattttgt
     2701 ccccagttct caattgacct ttaaactgat gtactccgtt tattaccaac ccgtttgaac
     2761 ttccggcgtc gtgtaacatt gccactccac tggcagtcaa aataagttcc gcgtgagtac
     2821 gggaagcttt aatgtccgtc agaacgatgt cattttcttc accgcgacca atggatacgc
     2881 ggttcactag caatacaaac acgaccccgg tatcgggtcc tccgacgacg cggagccgcg
     2941 ctatttcacc ctgctggcga gcgacggtca tgtttttaaa gcgtagaacc ttcttactca
     3001 atcgatcatc cttcccgctc cgcgattaat acaaacccct tagacggcgt tccctcaaaa
     3061 ccaagaccgt gacactcgaa ccggtgagct gctcccgata cttcgaaatt ttcctgaacc
     3121 acccctgcag aaccgggctg tgttcgatct aacaaatcct caacaaacgc agcgaatgcc
     3181 gattcccgcg ctacgtttcc aatttcagtg cccatggccg agtccccgcg aattcccgtg
     3241 agctcttcca taaatgcatt gagcgaaatt attcgtttgt cttgcgaaaa gaccatcacg
     3301 cccgaatttg ttaccttctc gcagatcacc ttcaatacgg ttaacgccaa ttcgcccgat
     3361 acgccttcgt tgccgccaag cggttgcgcc atcccaccgc cgatccgttg cagtcccgcg
     3421 ttgattacgt cgatcagcga gtccatctca ctcattttaa attttctagt gacaacgtga
     3481 ctatttccct tcagcacaga gtctacctct tcattcaaat gctgaatggg gcgtaaggtt
     3541 aaacggtata ttgaagcaaa cgcagcaacg gcaaaaatcg ccgctaatat cgcagaatag
     3601 aaaaacatta accactcggt gccagaatcg aaagttacaa gtcgctgatc aaagaaaacc
     3661 atacccaatg ctatggttac atttttgcct tgagctggat tcattacccg cacgggttcc
     3721 gcgaccgcga taattcttcc gtcgccttcg aagtacattc tgttacgctc tgggtcctga
     3781 aacgcttttc gggccgccgc agcgaatgac gcctgaaacc gatcttcggt aatatactgg
     3841 tttaacttgc gaccgggcgc caacactcgg ccttcaatat caattaggta ggccgcatag
     3901 accccgcgtt ctttctcggc aaaggtcaca tcggttttgg tttccatccg ctctaaaata
     3961 aaagatgtat tgcgatcaac aatttgtcgt gccagcagtc cggcgcggcc gcgcgcctct
     4021 gactggagct tctgttcggt tcgcgacacg acgagaaaaa ctgaaatgct aatgctaaat
     4081 agtatcgaaa atccgaagat cgtcaaaacg atcgatcgcc attcgtggcg ttcgtttaag
     4141 ttatagagaa aattaattac gtgtttttca aaattaaact tcaatttttc gagtagcgaa
     4201 gtcttcgcgg gcgtcgcgcc agaactagaa tttactgatg cgcctcctga gactggggtg
     4261 cccgagagcg gcagctgatc tgcgggcaac atatttgacg gcaccaacgc
//
LOCUS       DAOMXO010000122         4291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11061_length_4291_cov_5.800992, whole genome shotgun sequence.
ACCESSION   DAOMXO010000122 DAOMXO010000000
VERSION     DAOMXO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11061_length_4291_cov_5.800992"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..346)
                     /locus_tag="PLH57_03600"
     CDS             complement(38..346)
                     /locus_tag="PLH57_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01723.1"
                     /translation="MAKHHSEEFFSFDLDTLRAGEPVTFEVYLYLPLNEHVIRLIAPP
                     DVIEGTFIERFKKKGIRQLHIRNQDHAKYVEYLKAKTIAPTPAAPSAPPASITPVPPP
                     "
     gene            432..2231
                     /locus_tag="PLH57_03605"
     CDS             432..2231
                     /locus_tag="PLH57_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRK01724.1"
                     /translation="MIYIIGMFRQPSRKSENRWSDLLVLGSVAALIYAGVTLAARWTG
                     EGQMAVEIDLHPSALPKYALFSFSRAAIAYALSLAFSLVYGYCAAKARRAERVMIPLL
                     DILQSIPVLGFLPGLVIGLMAVFPKSNVGLELACILMIFSGQAWNMTYSFYSSVKAVP
                     QDLSEACGVMRLSWMERLKRLELPFAAIGLAWNSLMSMAGGWFFLTVCESFPLGERKF
                     QLPGVGSYMAVAIEQGDHRAMVFGIGAMVGIILFVDFLIWRPVMVWVRKFRMEEVHDE
                     VASLPFVTVLLRDSVLIRKLKPFWSRKHPARAGRRRRRIAGAVGLKAARQSTAPLMPI
                     PVDSGGSETYRESSRVGWKILSELATPLFALGCLWAAIQLWELVRELTLRDWRLIFSS
                     TLLTAARVGVAVLLGSLWTIPFGILVGSSPKLLRFFQPIVQVVASFPAPMLYPLALMI
                     MFKAGITLHWGSAILMMLGVQWYILFNVLAGGMKITNELRDGLTILSASKKVLWKRLY
                     LPAVFPTLVVGWFNAAGGAWNASIVAEHIHYDGRLNAVPGIGSMISRAAAAGNFPLLA
                     GSLLVMVGAVLLINNTLWRWLSDLAETRFRMDR"
     gene            2239..3546
                     /locus_tag="PLH57_03610"
     CDS             2239..3546
                     /locus_tag="PLH57_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HRK01725.1"
                     /translation="MSETGKAIIELKNISHTFRMGGGQKLRVLQDINISLYQDEVVAL
                     LGPSGSGKSTCLRILAGLLQPTHGRVLVDGKKLNGTNPRVSMVFQSYALLPWLTVFEN
                     VEVALESLQLPREEVRERVKRAIDLVGLEGFEEAYPRELAGGMKQRVGIARALVGERP
                     VLCLDEPFSALDVLTAEALRSEVLRLWLTRKTATKTMVLVTHNISEAALLAKRILVMG
                     TGPGHVQVSIKNDLPYPRDERSSGFKSLVMNIHDVITQSIIPDTPEWVPPALAATTIE
                     TIPPVPLVEVIGLIEFIAARGGQGDSFAIANELKKDFGNVLFLAKAAELLDFVDTPKN
                     AIVLTDFGKRFIQSDVNVRKRVLHEALKGLRLAQLLEQKLKTAEQFTLPIETVHEYIR
                     EWMPNENAETITNTLVQWGRYGELFGYNDDTKQIYLDVGQETA"
     gene            complement(3543..>4291)
                     /locus_tag="PLH57_03615"
     CDS             complement(3543..>4291)
                     /locus_tag="PLH57_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01726.1"
                     /translation="NDRYAWAMVTDYSFRSDLFDDVAIASLQATDEKLVFDYDVYDEY
                     QSMVASLLSYSNLDQKHRKKLFLKRLRRLLNDTSSARLSVTIDWLARTLDFAEDEIGK
                     DIRKIMNSAAGIRALEKLRVAELRLSDSAYLLWSNTPAMGKTEYWTKFGFVYLNSLCF
                     DDESSDPDTQNALRAFKKVKFSSPELFANKIIGWLQGYFANGGLRRWLDLGQLRKLQP
                     KVAEVLCLKLANASLVKDITISGCDDEAAP"
BASE COUNT         1011 a          939 c         1193 g         1148 t
ORIGIN      
        1 ctggcgctgg gcggcagctt cgaggcgaca ccggcggtca cggcgggggc actggagtga
       61 tcgacgccgg tggagcgctc ggagcagcgg gagtgggggc tatcgttttt gctttgagat
      121 actctacgta cttggcgtga tcttggttgc gtatgtgcag ttgtcggatg ccctttttct
      181 taaaacgttc gataaaagta ccttcgatca catcgggtgg ggcaattagc cgtatgacgt
      241 gttcgttaag tggaagatag aggtacacct cgaacgtcac gggttcgccc gcgcgcaggg
      301 tgtcgagatc gaaactaaaa aactcctccg aatggtgttt ggccaaaaaa acacactcct
      361 tcgtatgctt gtcggaggaa tcaaaaacga cgttcacaca tagaaaaaaa tgccgggcca
      421 atcttgcctt gatgatctat attatcggta tgtttcgcca accaagtcgc aaatcagaaa
      481 atcgttggtc ggacctgctc gtattggggt cggtcgcggc actcatttat gctggcgtga
      541 cattggcggc tcgctggact ggcgaggggc aaatggcggt cgaaatcgac ttacatccga
      601 gtgcacttcc taagtacgcg ctgttttcgt tttcccgcgc tgcgattgct tacgcgctct
      661 ctctcgcctt cagcctggtg tacggttact gtgctgcaaa ggcacgtcgg gccgaacggg
      721 ttatgatacc gttgctcgat attttgcagt ccattccggt tttgggtttt ttgccagggc
      781 ttgttatcgg gttaatggcg gtttttccga aaagcaatgt gggcctcgag ctcgcttgca
      841 tattaatgat ctttagcggc caggcctgga acatgaccta tagtttttac agctcagtca
      901 aggcggtacc ccaggaccta tccgaagctt gtggtgtaat gcgattgagt tggatggagc
      961 gcctaaagcg cttagaatta ccgttcgccg cgatagggct ggcttggaac agcttaatgt
     1021 cgatggccgg agggtggttt ttcttaaccg tgtgtgagtc gtttccactg ggtgaacgaa
     1081 agtttcaatt gccgggagta ggttcctaca tggcagttgc gattgaacaa ggcgatcatc
     1141 gtgcgatggt gtttgggatc ggcgcaatgg ttggaattat tttgttcgta gactttctga
     1201 tttggcgccc cgtgatggta tgggttcgaa aattccgcat ggaagaagtt cacgacgaag
     1261 tggcttcgct tccgtttgtt accgtgttgc ttcgtgattc ggtccttatt cgcaagttaa
     1321 aaccgttctg gtcgcgcaaa catccggcaa gagctgggcg tcggcgtcgg cgaattgctg
     1381 gggccgttgg cctaaaagct gcgcgccaat cgacagcgcc cctaatgccc attccggtag
     1441 atagcggagg aagcgaaaca taccgtgaat ccagccgagt aggttggaaa atactttcag
     1501 aactagcaac tccgcttttt gcattgggtt gtttgtgggc cgctattcaa ttgtgggaat
     1561 tggtgcgcga gctcacgctg agagactggc gattaatttt ttctagtacg ttattgacgg
     1621 cggctcgagt gggggttgca gtcttgttgg ggtctttgtg gacaattcca tttggaattt
     1681 tggtgggatc gtcgccaaaa cttctgcgat tttttcaacc gattgttcaa gtggtggctt
     1741 cgtttcctgc gccaatgctt tatccgcttg ctctcatgat tatgtttaag gcgggtatca
     1801 cccttcactg gggctccgct atactgatga tgttgggcgt gcagtggtac attttattca
     1861 acgtcctcgc cggaggaatg aaaatcacaa atgaactgcg agatggattg acgattttgt
     1921 ctgcttcaaa aaaggtgctc tggaaacgac tttatttgcc ggcagtgttt ccgactttgg
     1981 tggtgggttg gtttaatgca gcgggtggtg cttggaacgc cagcatcgtc gcagaacaca
     2041 ttcactatga tggacgtctg aacgccgtac cgggaattgg ttccatgatt agtcgggcgg
     2101 cggcggctgg aaactttcca ctgcttgctg gtagtttatt ggtgatggtg ggagccgtac
     2161 tgttgattaa taacacgctg tggcgatggt tgagtgatct agcagaaact agattcagaa
     2221 tggatcggta gggacattat gagtgaaacc ggaaaagcga tcatcgagtt aaaaaatatc
     2281 agtcacacgt ttcgcatggg cggtggccaa aagttaaggg tactgcagga tattaacatc
     2341 agtctttatc aggatgaagt tgtggcattg ctcggaccgt cgggatcggg caagtcgacg
     2401 tgtttgcgaa ttctcgccgg attattgcaa cccacccatg gtcgcgtttt ggtagacggc
     2461 aaaaaattga acggcaccaa tccgagagta tcaatggtgt ttcagtctta cgctttgctc
     2521 ccctggctta ccgttttcga gaacgtcgaa gtcgctctgg agtcgttgca gttgcctcga
     2581 gaagaagtgc gcgagcgagt gaaacgagcg atcgacctag taggtctgga ggggttcgaa
     2641 gaagcatacc caagagaatt ggcagggggc atgaaacaac gtgtcggcat agcacgagct
     2701 ttggtggggg agcgaccagt gctttgtcta gacgaaccgt tcagcgcact cgacgttttg
     2761 accgcagagg cgctgcgttc cgaagtgttg cgtctttggc ttactcgtaa aaccgcgacg
     2821 aaaacgatgg tgcttgtcac tcataacatt agcgaagcag cattgctcgc aaagcgtatt
     2881 ctggtaatgg ggactgggcc gggtcatgtt caggtttcta taaaaaatga cctaccgtat
     2941 ccgcgtgatg aacgaagttc ggggtttaaa agcttagtca tgaacattca tgatgtgatt
     3001 acccagtcga ttattcccga tacaccggag tgggtgccgc ccgctctggc tgcgacaact
     3061 attgagacga ttcctccagt gccgctggtc gaagtgatcg gcttgattga gtttatcgcg
     3121 gcacgcggtg ggcaaggcga cagttttgcc attgcaaatg aattgaaaaa agattttgga
     3181 aacgtactgt ttctcgcgaa agctgctgag cttcttgatt tcgtcgacac tccaaaaaac
     3241 gccatcgtat taaccgactt tggcaagcga ttcattcaga gcgacgtgaa cgttcgaaaa
     3301 agagtgctgc acgaagcact caaggggttg agacttgcgc aacttttgga acagaaactg
     3361 aaaactgccg agcagtttac gctaccgatt gaaacagtgc acgagtacat tcgcgaatgg
     3421 atgccaaatg aaaatgctga aaccattact aacaccctgg ttcagtgggg ccggtatggc
     3481 gaactgtttg gctataacga cgataccaaa cagatatacc tagatgtagg gcaagaaact
     3541 gcttaaggtg cggcctcgtc gtcgcaacca gaaatggtaa tatctttaac taggctggca
     3601 ttagcgagct ttaaacaaag tacttctgct accttaggct ggagttttcg tagctgccct
     3661 aggtcgagcc agcgacgaag gcccccgttg gcaaaatacc cttgaagcca gccaataatc
     3721 ttgttggcga ataattcggg actactaaat ttgactttct taaatgcgcg cagggcattt
     3781 tgggtgtccg gatctgaaga ttcgtcgtcg aaacagagac tattaagata tacaaatccg
     3841 aacttggtcc aatattcagt tttacccatt gctggcgtat tgctccagag taagtaagcg
     3901 ctatcgctca gccttagttc ggctactcga agtttttcta acgcacgaat tccggcggcg
     3961 gagttcatga ttttacgaat atcttttccg atttcgtctt cggcaaaatc gagagtccta
     4021 gccaaccagt cgatcgttac agaaagcctg gcactgctgg tgtcgtttaa tagtcttcga
     4081 aggcgtttta gaaataattt ttttcgatgt ttctgatcta gattggagta agataataaa
     4141 ctcgcaacca tagactggta ttcatcatag acgtcgtagt cgaagactaa tttttcatcg
     4201 gtagcctgga gagacgcaat agcgacgtca tcgaagagat cgctgcgaaa agagtaatca
     4261 gtaaccatag cccaagcgta gcggtcgttc g
//
LOCUS       DAOMXO010000123         4272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11138_length_4272_cov_4.203225, whole genome shotgun sequence.
ACCESSION   DAOMXO010000123 DAOMXO010000000
VERSION     DAOMXO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11138_length_4272_cov_4.203225"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1948)
                     /locus_tag="PLH57_03620"
     CDS             complement(<1..1948)
                     /locus_tag="PLH57_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01727.1"
                     /translation="MKKRRILFLFFSSVFLFVMTILSFIQSEMFARILKSIAQRQLPL
                     DLGVEGEFRDLGVKLLPPGIVIHKPALELKEKNFAGLPGGTKIEAEALDLTFQFFQLL
                     TGEVNVNAFAIVGANLTLDLDEKFFSNYEKRLKEQQAGSVMGIKVPSISISWDHLFRF
                     KFRSVSIQDSKVDIRLHRGGNFIPVSVQALAKELTVGQNTIDGFPAYDIGIDFQNASL
                     KIGDFDQTLAQFQSSIELSSRGANVRMLSMQEQDMNLHANGAIVGNILNPTQLMSDIN
                     YIIKGPIPKLLSPKMLGFFVKLPKNTRADGMLTLEGNAKSDLLKLIPSIESKFTLSIA
                     NPFYDGWSARKVSVAGEWRRPVLAMTGAEVEFDEGGVKFSRIDYDSRADLEKISVVAK
                     LENADFRKLLGPAVNNVFQIHMFLSGQLTSEIDLRNKIKVVGSTTLDITNFSFDNQKP
                     NIVKPFKQLLSIPKMGLKAGFALDEHGARFSDTELKIDQTKLTATGKVDGSGMFDIDI
                     RGPIKLEEIGKLSAFEIGGEGNLHWTIKGKKPHVLFSFDAQAKNTRYLNMNLGTVAGK
                     IIYDDGSEILTISKLKSQIHRSEFEANGTVDLGKSDVIDVDILVPGATIPDLSFIFER
                     FIRASVPWFPWDLNGRMDGRIKVSG"
     gene            complement(1945..2826)
                     /locus_tag="PLH57_03625"
     CDS             complement(1945..2826)
                     /locus_tag="PLH57_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK01728.1"
                     /translation="MDQKKNRPDGTTVDLGDDLLEATRLPVGQNKVAPNQVPDVIESI
                     PDSEKNPEASESSDEKLMRAEILIGERLFEDAKKILRQMLKADPNSAIVKEKLLEIQK
                     GEIQELLVDGGRKKLTVEKSEQKVESSDEVLERLERDLRLNISGGVKPVPTLFATPVA
                     YEKYCNRVVAEVIRLPVRNRIDLGIAHLEMGLFEVAVAVFETVVRYEDQKLEGTYLMC
                     LALIFGGRAIEATIRLEPLVRDLTMPEDDKTDFMYLMALAFEKLGDIRKAKEFYRRAY
                     TLNPRYRDVAEKLNELK"
     gene            complement(2842..4182)
                     /gene="accC"
                     /locus_tag="PLH57_03630"
     CDS             complement(2842..4182)
                     /gene="accC"
                     /locus_tag="PLH57_03630"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRK01729.1"
                     /translation="MFKKILIANRGEIALRIIRACREMGIATVAVHSKADEESLHVKL
                     ADESVCIGPPQSKLSYLNVAALVSAADLCGVDAIHPGYGFLAENADFARICEQCNVTF
                     IGPKPEQISKMGDKATARRIAKEAGVPMLPGTGILRDVEDAARRADEIGYPVMLKATA
                     GGGGRGIQIIYHPSQLRTSFERVRSEAAAAFGLGDCYMEKFCEHPKHIEIQVLCDGKG
                     NNLSLGERDCTVQRRHQKLIEEAPSPVVDAELRKKMSDAAIRLCQAVDYLGVGTVEFL
                     VDEKKNFYFMEMNTRIQVEHPVTEMVTGIDLIKKQILVAAGYGHGLKNEDVVINGHAI
                     ECRINAEDPEKMVPSPGVISTYHAPGGYGVRVDSFVYQNYRVQPFYDSMIGKLICHAP
                     TRDEAIAKMSCALSEIVVGGIHTNVAFQRKIILHPKFKTAEYDTHFLEEVLAKY"
BASE COUNT         1102 a         1033 c          882 g         1255 t
ORIGIN      
        1 acccggacac cttaattcga ccgtccatgc ggccatttaa gtcccatgga aaccaaggaa
       61 ccgatgcacg aataaaacgc tcgaaaataa aggacagatc aggaatggtt gccccgggaa
      121 caagaatgtc gacatctata acgtcacttt ttccaagatc gaccgtaccg tttgcctcaa
      181 attcacttct gtgaatttga gacttcaatt tgctaattgt gaggatttca gaaccgtcgt
      241 cgtaaattat tttacctgct acggtaccca aattcatatt caggtaacga gtattcttcg
      301 cttgtgcgtc aaacgaaaac agcacgtgtg gtttctttcc cttaatggtc caatgtaaat
      361 ttccttcgcc accaatctcg aatgctgaca actttccgat ttcctccaat ttaattggtc
      421 ctcggatatc tatgtcaaac attccggatc cgtcgacttt tccagtcgca gtcagcttcg
      481 tttgatcaat tttcaactcc gtgtctgaaa atcgcgcacc gtgttcatct agcgcgaagc
      541 ccgctttaag acccattttc ggaatagaaa gaagctgctt aaagggcttc acaatgttgg
      601 gcttttggtt gtcgaaagaa aagttggtga tgtcgagcgt ggtggagcca accactttta
      661 tcttatttct caaatcgatt tccgaagtta attgtccact taaaaacata tgaatctgaa
      721 aaacattatt caccgccgga ccaagtagct ttcgaaagtc cgcgttctcc aacttagcta
      781 ctacagagat tttctcgaga tccgccctag agtcgtagtc aattcgacta aattttacgc
      841 caccctcatc gaattccact tctgcaccag tcatcgcgag aaccggtcgc cgccactcac
      901 cggctactga cacctttcgt gcgctccaac cgtcataaaa tggatttgcg atgctaagtg
      961 taaattttga ttcgatactg ggaatcaact ttaataaatc tgatttagca tttccttcga
     1021 gtgtcagcat tccatcagca cgggtattct tgggtagctt tacaaaaaac cccaacattt
     1081 tcggcgacaa taatttagga attggtccct taattatata gttgatgtca gacattagct
     1141 gagttggatt taaaatattt cctacgatgg cgccgtttgc atgaagattc atgtcttgct
     1201 cttgcatgct cagcattcgt acattggctc ctctagaaga caattctatg ctggattgaa
     1261 actgtgctaa agtttgatcg aaatcgccaa tcttcaatga agcattttga aaatctattc
     1321 cgatatcata cgcaggaaat ccgtcgatcg tattttgacc aacagtgagt tcctttgcta
     1381 aagcctgtac tgatactggt ataaaatttc caccgcgatg aagtcgaata tcgaccttgg
     1441 aatcttgaat acttacgctg cgaaatttaa atcgaaaaag atgatcccag gagatcgata
     1501 tcgacggcac cttaatgccc atcacggaac cggcctgctg ttcctttaat cttttttcgt
     1561 aattcgagaa aaacttttca tctagatcta aagtcaaatt agcgccaacg atagcaaagg
     1621 cgtttacgtt aacttcgcca gttaaaagtt gaaaaaattg aaacgtaagg tccagtgctt
     1681 cggcttctat ttttgtcccg cccggaagac ccgcgaaatt cttttctttt agctcaaggg
     1741 cgggtttgtg aataactatt ccgggtggta acaactttac ccctaagtcg cgaaattcac
     1801 cttctacacc gagatctaaa ggcagctggc gctgcgctat gctttttaat attcgagcga
     1861 acatctcact ctgaatgaac gagagaatcg tcatcacgaa gagaaagacg gagctgaaaa
     1921 ataaaaagag gattcgtctc tttttcattt gagttcgttt aatttctcgg cgacgtctcg
     1981 gtaccgcggg tttagtgtgt aggcgcgtcg gtaaaactct ttcgccttac gaatatcccc
     2041 tagtttttca aaggccaatg ccattaggta cataaaatcg gttttatcgt cttcaggcat
     2101 ggttaaatcc cgcaccaagg gttctaaccg aatcgtagct tcaatagcgc gccctccaaa
     2161 aatcagagcc aggcacatga gataggtacc ctctaatttc tgatcttcat acctgacgac
     2221 agtttcaaat acagctacgg cgacttcaaa aagccccatt tcgagatgag cgattcctaa
     2281 gtcgattcta tttctaaccg gaagacgaat cacttcggct accacacgat tgcagtactt
     2341 ttcgtaggcg accggagtgg caaataatgt aggaaccggt ttgacaccgc cgctaatatt
     2401 taagcgcaga tcgcgttcca atcgttcgag cacttcatct gagctttcaa ctttttgttc
     2461 ggatttttcg acagttaatt ttttccgccc gccatctacc agcaattcct gaatttcacc
     2521 tttttgaatt tccagtaact tttcttttac gatcgcgcta tttggatcgg ccttgagcat
     2581 ttgacgtaga attttttttg cgtcttcaaa aagtctttca ccaattaaaa tttcagcgcg
     2641 cattaatttt tcatcactac tttcgctggc ttccggattt ttttccgaat cgggtatcga
     2701 ttcaattaca tcgggcactt ggttcggtgc gactttattt tgtccgacgg gcaatctcgt
     2761 ggcttcaagt aagtcatcgc caagatccac ggtggtgccg tcgggtcgat tctttttttg
     2821 gtccataaca gattaatttt actagtattt tgcgagaact tcttccagaa agtgcgtgtc
     2881 gtactctgct gttttgaatt ttggatggag aataattttc ctctgaaaag cgacgttcgt
     2941 atgaataccg ccgacgacaa tttcagatag cgcacaggac attttggcaa ttgcttcgtc
     3001 tcgcgttggg gcgtgacaga taagtttgcc tatcatcgag tcgtaaaacg gctgaacccg
     3061 gtaattttga tacacaaacg agtctacgcg aacgccatat ccacctggtg catggtaggt
     3121 agaaatgacc ccaggactag gcaccatttt ctcagggtct tcagcgttga tacgacactc
     3181 aattgcatgg ccgtttatta cgacgtcttc attttttaga ccgtggccgt acccggcggc
     3241 gactaggatt tgtttcttaa tcaaatcgat tccagtgacc atctccgtta ccgggtgctc
     3301 aacctgaatt cgggtattca tttccatgaa ataaaagttc tttttttcgt cgactaaaaa
     3361 ctcaactgtg cctacaccta aatagtcgac cgcctgacaa agtcgaatcg cagcatcgct
     3421 catttttttt cttaattcgg cgtctactac tggtgaaggt gcctcttcga ttagtttctg
     3481 atggcgacgt tgcacagtac aatctcgttc acccaaactt aaattattgc ccttgccgtc
     3541 gcacaatacc tgaatctcaa tgtgcttagg atgttcacaa aatttttcca tgtaacagtc
     3601 gcccaaacca aaggctgcgg ctgcctcaga acgtacgcgt tcaaacgatg tccgaagttg
     3661 cgaagggtgg taaattattt gaattcctct ccctcctccg ccggcggttg ctttgagcat
     3721 tactggatac ccgatttcat cagccctacg ggcggcatct tctacatcgc gtaatattcc
     3781 agtgccggga agcatgggaa caccagcttc ttttgcaatt cgtcgcgcag tagctttgtc
     3841 acccattttc gaaatttgtt cgggcttggg cccaataaac gtcacattac attgttcaca
     3901 aatacgcgca aaatcggcgt tttccgctaa aaatccgtaa ccgggatgaa tggcgtccac
     3961 tccgcataga tctgcggcac taactagggc cgcaacattt agatagctga gtttggattg
     4021 ggggggaccg atacatactg attcatcagc aagttttaca tgcaggcttt cttcgtctgc
     4081 tttggaatga accgcaactg tggcaatgcc catctcgcgg catgcgcgaa taattcgcag
     4141 cgcaatttcc ccgcgatttg cgataagaat ctttttaaac atatctgaaa aacttaaacg
     4201 cagcccgtgc gcacgtgctt gggcaccccg ctgccgtgtc cgtgcaggcc ctggcgcgcg
     4261 gcgcgctggc cg
//
LOCUS       DAOMXO010000124         4268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11151_length_4268_cov_4.699976, whole genome shotgun sequence.
ACCESSION   DAOMXO010000124 DAOMXO010000000
VERSION     DAOMXO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11151_length_4268_cov_4.699976"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PLH57_03635"
     CDS             <1..1192
                     /locus_tag="PLH57_03635"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HRK01730.1"
                     /translation="LRHCTLELSIWNVASFFSKIDRFDSKMGDRPIWVLLLLGLASIP
                     LIPWAPFFGYDLYNIQVYHKCPPVQWNPYLPESVQCGDHAGRSMPYPPLLYFSFIWTK
                     YLSPATSKIIWGVVLIALMSGSLLWWLRQEREDRKQISRQDLAFGLLLLLQFPFIFTL
                     ERANSDVPVVALWTVATWAATKNRWAVAGMAAGAAVTFKIYPIIALGVISLGTVITLA
                     KEKLRKHGLVLGLVSALTMFIGFYLLWPQDIWNNWLVTIGGYNTGGSGTFYYSHTLSR
                     PFGGKYWIPALIIRTALFGIFVWATKAKIKTDPRLVFAGALAMTTYYTGTSYDYNLVT
                     IYPLFILLYVRKLWSLLGIGLFFVCMARPLFVQSPGVQILGQIGWLMLIALYCSTSNR
                     TRKI"
     gene            1193..2119
                     /locus_tag="PLH57_03640"
     CDS             1193..2119
                     /locus_tag="PLH57_03640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK01731.1"
                     /translation="MKLFITTPVYFDVPSFRILRDRVRSVSIQSKIGTPEFLVIDDSG
                     GNDTEIDSLRNDSDTRIINVPFNMGSQRAFVYSLRLLCKDIGPEDIVVTMDADGEDQP
                     EHIPALVETLNNSTENNRVVLARRKRRRDPYWFKVGYVLFKWIFRGLIGTEISTGNFA
                     AMRGHFVQTLLWHPHFDLVYAGGLVSVNTPKSFVPCERGPRYAGESKMTYLNLCMHAV
                     RMLMPFLDRIAIRAALFSLILMFASSMILATNWIFPDFFSTLPKWLPLLAQAVWLGSG
                     LLLLSSLTIFISFIHHQSALVRALQRSFVPRV"
     gene            complement(2099..3352)
                     /locus_tag="PLH57_03645"
     CDS             complement(2099..3352)
                     /locus_tag="PLH57_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01732.1"
                     /translation="MFKFIATAFVLGLPVLAQAEVPSLAIGAEVLKSPCPAGPDTNFN
                     LTRAYYPEVFNSLDQNVYSNAKVLNCENDPNYYILAGTTGIQQEEKESFPAFYGNTAP
                     GYKVVRTIPKKSVESLYVFSFGSPLIARGIDATHLECDRNVDHEENNQLCADLDDITR
                     KTAVGGLYHYFYVDHGKSKNCEGKYELFASNLKSIRNYDESMEKSVYEMQNSSALGVC
                     FNDEKSFQQLREKPPVEPLKILDFKWKNDDVVDLLVYRKRKVAGSRKDEISLLNYRVD
                     LAAGKASEQYKISGEISEIEIFPNSCGEYALTARILGDGNVAIFYPVPRVVFVNVQNA
                     NKLRAKIEYEGSSRLMTCEGGGPRFEYGRSRFINNGYGYSYDIGAKISFVKYPYGENY
                     TGIHWWVNGQGDDDHLITLSNSWHK"
     gene            3553..3987
                     /locus_tag="PLH57_03650"
     CDS             3553..3987
                     /locus_tag="PLH57_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019480129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRK01733.1"
                     /translation="MNTPTIARELSKTWNVEQNPIVELEAPHSDARGSIIPLVDETMR
                     SAVLITSKKNTVRANHFHHTDWHYCYVLKGAIDYYYRPTGSNLPPKCTRVSQGQMFFT
                     PSQCDHAMHFVEDTEFLCLGRNPRDQATYEADITRIELFNAP"
     gene            3992..>4268
                     /locus_tag="PLH57_03655"
     CDS             3992..>4268
                     /locus_tag="PLH57_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRK01734.1"
                     /translation="MNVKATPKSSACHRRIDCRLCGSKTLTQVLKLAPTPPANALVTK
                     EQLGESEERFPLDVFICDACSHVQLLDVVNPEVLFRNYVYVSGTSPVF"
BASE COUNT         1088 a         1026 c          902 g         1252 t
ORIGIN      
        1 cctaagacat tgcaccttgg agctttctat ctggaacgtc gcatcttttt tttcgaaaat
       61 tgatcgtttt gactccaaaa tgggggaccg ccccatatgg gtcctgcttt tacttgggct
      121 tgcgtcgatc ccattaattc cgtgggctcc tttttttggt tatgatctct acaacattca
      181 agtttaccac aaatgcccac cggttcagtg gaacccttat ttgccagaat cggtccaatg
      241 cggcgatcac gccggtcgca gcatgcccta tcctccgctg ctttatttta gtttcatttg
      301 gactaaatat ttgagtcctg ctacttcaaa gatcatttgg ggcgtggtct taatagctct
      361 catgagcgga tctctacttt ggtggctgcg ccaagaaaga gaagaccgca aacaaatatc
      421 ccgccaagat ctcgcttttg gattgctctt acttttacaa tttccgttca ttttcacgct
      481 agagcgagct aacagcgatg tacccgttgt cgcgctttgg acagtcgcta catgggccgc
      541 tactaaaaat aggtgggccg tggcaggaat ggctgccggc gctgcggtaa cttttaaaat
      601 ttatcctatc atagcacttg gggtcatttc gcttggcaca gtgatcacct tagccaagga
      661 aaagcttagg aaacacggtc tcgttttagg acttgtttcc gctcttacaa tgttcattgg
      721 attttatttg ctctggccgc aggatatttg gaacaactgg cttgtaacga taggcggcta
      781 caatacaggc ggttcgggta cattctatta ctcgcacact ctttctcgcc cttttggggg
      841 taaatattgg atacctgctc taattatcag aacagccctt ttcggaattt ttgtttgggc
      901 tacaaaagca aaaattaaaa cagatccacg tttggttttt gctggtgccc tagctatgac
      961 cacttactat accggcacaa gctatgacta caacctggtg accatctacc ctttgtttat
     1021 attgctctac gtcagaaaac tctggtcttt attgggtatt ggcctatttt tcgtgtgcat
     1081 ggctcgcccc ctgtttgtgc aatcaccggg ggttcaaatc cttggacaga ttgggtggct
     1141 catgttgatt gctctgtatt gttcgacaag caatagaact cgcaaaatat aaatgaaact
     1201 atttattacc actccagttt atttcgatgt tccgtcattt cgaatactgc gcgatcgtgt
     1261 tcgatcagtt tcaatccaat caaaaattgg aaccccagaa tttctggtaa ttgacgattc
     1321 tggtggcaac gacacagaaa tagattcctt acgcaatgat tccgacacgc gaatcattaa
     1381 cgtaccgttt aatatgggca gccaaagagc cttcgtttat tccctccgac tactctgtaa
     1441 ggacattggc ccagaagata ttgtggtcac tatggacgct gatggtgaag atcagcccga
     1501 acacattcct gccttggtag aaacgcttaa taatagcact gaaaacaacc gagtagtctt
     1561 ggcccgtcgc aaaagacgtc gtgatcctta ttggtttaag gtgggatatg ttttatttaa
     1621 atggattttt cgaggtttaa tcggtaccga aatctcaact ggcaattttg cagccatgcg
     1681 tggacacttt gttcaaacgt tactttggca ccctcatttt gatttagttt atgcgggcgg
     1741 actggtgagc gtgaatactc cgaagtcttt cgttccttgt gaacgaggtc cgcgttatgc
     1801 tggcgaatca aaaatgactt atttgaatct ttgcatgcat gccgttcgca tgctcatgcc
     1861 ttttttagac cgaatcgcga ttcgagccgc attattttca ttaattttaa tgttcgcaag
     1921 ttctatgatt ctggccacaa attggatttt tccagatttt ttttcaaccc ttccaaagtg
     1981 gttaccattg ctcgcacaag ctgtgtggct tggatctggc ttgctattgc tgtcatctct
     2041 aacgattttt ataagtttta ttcatcacca aagcgctttg gtgagagcct tacaacgctc
     2101 atttgtgcca agagtttgac aatgtaatca ggtgatcgtc atcgccctga ccattcaccc
     2161 accagtgaat gcccgtatag ttttcgccgt atgggtattt cacaaaggaa atctttgcac
     2221 caatatcgta agaatagccg tagccgttgt ttatgaatct cgagcggcca tattcgaacc
     2281 ttggtccgcc accttcacag gtcatgagtc ttgagctgcc ctcgtattca attttggctc
     2341 tcaacttatt ggcattttgc acattcacaa acacgaccct cggtacggga tagaatatag
     2401 caacgttccc atcgcctaaa atacgtgcag ttagtgcgta ctcgccgcag gagttaggaa
     2461 aaatttcgat ctcgcttatt tctccggaaa ttttatactg ctccgaagct ttgcccgctg
     2521 ctagatcgac gcgatagttt aacaatgaaa tttcgtcttt acgtgaaccc gcaaccttcc
     2581 tcttacgata aacaagtaga tcgaccacgt cgtcgttctt ccatttaaaa tcgagaattt
     2641 ttaggggttc gactggaggt ttttctctca actgctgaaa gctcttctca tcgttaaaac
     2701 aaacgcccaa agcggaactg ttttgcattt cgtagacgct tttttccatg ctttcatcat
     2761 agtttctgat cgactttaga tttgaagcaa acaattcata tttgccctca cagtttttgg
     2821 acttaccatg atccacataa aagtagtggt aaagccctcc gaccgcagtc ttcctagtga
     2881 tgtcatctaa atcagcgcaa agttggttgt tttcttcgtg atcgacgttt ctgtcacact
     2941 caaggtgggt cgcatcgatt ccacgcgcaa ttagcggtga cccgaacgaa aaaacataaa
     3001 gcgattcaac cgattttttg ggaatagttc gcacgacctt atagccgggg gctgtgttgc
     3061 cataaaaagc agggaacgat tccttttctt cctgctgaat ccctgtagtt ccggcgagaa
     3121 tgtaataatt agggtcgttt tcgcagttca aaacttttgc gttggaatag acattttgat
     3181 ctaaagaatt gaacacttct ggataatagg cccgcgtcaa attgaagttg gtatcgggac
     3241 cggcaggaca cgggctctta agaacttcgg cgcctattgc gagactcggt acctcagctt
     3301 gagcaagaac tgggagccca agaacgaatg ccgtagcgat gaacttaaac atagtgatcg
     3361 tccgcctttg aatgatggtt aaaagccctc aatggggcct acacagtaga agccgcgcag
     3421 tcaatcgaca ttcaattcca ttggcaattc cattggcaat tccattggca atttcgctgc
     3481 caaaaaatgc gctgcagtgc gcgtcgcgct ttcttgacaa agaacaaacc accttcataa
     3541 attgaggccc atatgaatac accaactatt gctcgcgaat taagtaaaac ctggaacgta
     3601 gaacaaaacc caatagttga attagaggca ccgcacagcg acgctagagg ttcgattatt
     3661 ccactggttg acgaaaccat gcgaagtgca gtcttgatta cctcgaaaaa aaacaccgtg
     3721 cgcgccaacc actttcacca caccgattgg cattactgtt acgttttaaa gggtgcgatc
     3781 gattattact atcgccccac cggaagcaat ctccccccga aatgcacccg cgtctcgcaa
     3841 ggacaaatgt ttttcactcc atcgcaatgc gaccacgcaa tgcactttgt cgaagacacc
     3901 gaattcttat gtctggggcg caacccgcgc gaccaagcca cttacgaagc cgatatcacc
     3961 cgcattgaac tatttaacgc cccgtaattc aatgaacgtt aaagcaacac ctaaatctag
     4021 tgcctgccac agacggattg attgccgact ttgtggttca aaaaccttga cccaggtctt
     4081 aaagcttgca cccacaccac cggcaaatgc attggtaacg aaagagcaac ttggcgaatc
     4141 tgaagaacgc tttcccctcg atgtttttat ctgcgatgca tgctctcatg ttcaacttct
     4201 ggatgtagtt aaccctgaag ttcttttccg gaactacgtc tacgtatcgg gcacgtcacc
     4261 agtatttg
//
LOCUS       DAOMXO010000125         4258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11193_length_4258_cov_4.209374, whole genome shotgun sequence.
ACCESSION   DAOMXO010000125 DAOMXO010000000
VERSION     DAOMXO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11193_length_4258_cov_4.209374"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLH57_03660"
     CDS             <1..750
                     /locus_tag="PLH57_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRK01735.1"
                     /translation="PTFEIPPNLGGRYSVLSAVGLFPAMVAGLDYHPFLTGAQEVVSH
                     FEDQVNAKVVPDALELAARLVQFYKKDGRNITVLMPYSQKLKTFSAWFVQLWAEGLGK
                     NGLGITPVAATGPTDQHSLLQILRDGPNDKTVGFIEVLGFQNLTELKWTGVTGGTFDL
                     VSGITLNQLMDAELNATRQVLSNQSRPHFSLCIPKLNPTTLGQLFQFLALTTALAGYL
                     LEIDPFDQPGVEEGKVLAIDRINACKKNAIV"
     gene            760..1674
                     /locus_tag="PLH57_03665"
     CDS             760..1674
                     /locus_tag="PLH57_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRK01736.1"
                     /translation="MSSKGSDEKTVVLSGESDTLKVEKERAKDQEACLIIIRGSPQGK
                     RYEINKDSMFLGRDVSAEIALNDQNVSRKHAEVIREGKEIKLRDNGSTNGTFVNDKLI
                     KDTVTLRKEDMIKLGNTILKFLPQGELEIFYLGTLESAAHTDPLTKVYNKGYISEALE
                     AEFKRARALHHDFSLIILDLDHFKKVNDTFGHDAGDYVLKEMCHLIRAKILPKSAIFG
                     RFGGEEFLILLPEHTLEATVQVAENIRSGIERHPFTYDGKKLPVTASIGVAESALDIE
                     SHTALFKLADKAVYQAKSSGRNQVCTAS"
     gene            1721..3556
                     /gene="mutL"
                     /locus_tag="PLH57_03670"
     CDS             1721..3556
                     /gene="mutL"
                     /locus_tag="PLH57_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRK01737.1"
                     /translation="METPLRRIHRLPSDLAEKIAAGEVIERPSSVIKELVENSIDAGA
                     TKVVVEIVDGGKESIRVIDNGSGIFPEDLPLALERHATSKITKLDDLWNLSSMGFRGE
                     ALPSVSAISRFTIESRVAGQVGRKLYLEGGILKSDQPLGATSAIASSGTSLTVENLFY
                     NVPARLKFLKAKSGETSQLRDLIERIALSHPGVHFTFSSDGRKSLDLPTCSSEAARVA
                     QVFGTSENDLEHFSTQFEGSQVWGYLDRNARMPNSRSVYLSVNHRMVRDKLLQQAVLL
                     ALRPRMMEGEYPKIFVQVLVPPNQVDVNVHPAKSEVRFERSRDVFQLVHGVIERSGRE
                     TNLAFYASPDRSYGWKEPSKINHAFSTEKLTPQPVAAAAMPLLAGDPAAARTVFRTKT
                     DIGFDSVPLANSAREQTSPFSRLQYIGQLKNTYLLCQDETGLVLIDQHAADERVQYER
                     LKKELSESGLKSQPLLISATHKCSDEDAALAIDYGDLFARLGLEIESFGTNCILIRTV
                     PEMLSQQHALELFQAMLAELKSLEGASSVSLVNDPTRHSAKLERLIATMACHSAVRAG
                     QALSEREARALMATMDRTDFALNCPHGRPASIQLSYSQVEGLFKR"
     gene            3559..>4258
                     /gene="miaA"
                     /locus_tag="PLH57_03675"
     CDS             3559..>4258
                     /gene="miaA"
                     /locus_tag="PLH57_03675"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRK01738.1"
                     /translation="MKTILCIVGPTASGKSELALRLAEEFDAEILSCDSLLVYKDLQI
                     GTAKPTDDEMRRVPHHGVNLVDANENYNAGDYVRYARGIIDDAVARKKKIIIVGGTGF
                     YLKALLCGTWDAPPTQPDLRAQFEPQDSQLLYAQLLEKDPSYAAKIQANDRYRVIRAL
                     EIIEVTGMRLSDQLAQKKPQNPLPYPSRILGIRRSKPDLEKRIIARTNDMFSRGLVNE
                     TKTLVERFSTSPSAL"
BASE COUNT         1138 a         1064 c          991 g         1065 t
ORIGIN      
        1 cctacttttg aaattcctcc gaacctgggt gggcgttatt cagtacttag cgcggttgga
       61 ctttttccag cgatggtagc gggactcgat tatcacccct ttctcactgg cgcgcaggaa
      121 gttgtctctc attttgaaga tcaggtgaac gccaaagtgg tgcccgatgc cctagagctt
      181 gccgcaagac tggttcaatt ttacaaaaaa gacggccgca atatcactgt tctgatgcca
      241 tactctcaaa aacttaaaac cttttcggcg tggtttgttc aactttgggc agagggtctt
      301 ggaaaaaacg gattaggaat cactccggtt gcagccaccg gtcccaccga tcaacattca
      361 ttgctgcaga ttttaagaga cggtcccaac gacaagacgg tcggctttat cgaagtctta
      421 ggttttcaaa atttaactga acttaagtgg acaggtgtaa ccggaggaac tttcgacttg
      481 gtgagcggaa taactctcaa ccaacttatg gacgccgaac tcaatgcgac tcggcaggtt
      541 ttgagcaatc agtcgcgtcc acatttcagt ctttgcatac ccaaactaaa tccgactaca
      601 ctgggtcagc tttttcaatt tctcgcactt accactgcgc tcgcgggcta tttgctcgaa
      661 atcgacccgt ttgatcaacc aggtgtggaa gagggcaaag ttctcgcgat cgaccgcatc
      721 aatgcttgca aaaaaaatgc aatcgtttag cctttaagca tgagctcgaa ggggtctgac
      781 gaaaaaacgg tcgttttaag tggtgagtcc gacactctta aggtcgaaaa agaacgagca
      841 aaagaccaag aagcctgctt gatcattatt cgtggcagcc ctcaaggcaa acgttacgaa
      901 atcaataaag attccatgtt tctgggccgc gatgtgtcgg ctgaaatcgc attaaacgat
      961 caaaatgtat ctcgcaaaca cgccgaagta attcgcgaag gcaaagaaat caaattacgc
     1021 gacaatggtt caaccaatgg caccttcgta aacgacaagt tgatcaaaga caccgttacg
     1081 cttcgtaaag aggacatgat caaacttgga aacactattt taaaatttct accgcagggt
     1141 gagcttgaaa ttttttacct tggcacgctt gaaagcgcag cgcacaccga tccgctcacc
     1201 aaggtttata acaaaggcta tatttcggag gctctagagg cggaatttaa acgtgcccgt
     1261 gcgttgcacc atgatttttc gttgattatt ttagatctgg atcactttaa aaaagtaaat
     1321 gacacattcg gtcatgatgc gggcgattat gtacttaaag aaatgtgcca cctgatacgc
     1381 gcaaaaattc ttccgaaatc ggcaattttc ggtcgatttg gtggcgaaga atttttgata
     1441 cttttgcccg aacacactct agaggccaca gtccaggtgg cagaaaatat tcgctcgggc
     1501 atagagcgac accccttcac ctacgatggc aaaaagctcc ctgttaccgc cagtatcggc
     1561 gtggccgagt cggcactaga cattgaatct cacactgcgc tttttaagtt ggcagacaaa
     1621 gccgtttatc aggccaaatc gtctgggcga aatcaggttt gtacagccag ttaaccagcc
     1681 ccttgccaag gtccgctcct tgtcactatg ttgaactttc atggaaaccc ctctccgtcg
     1741 cattcatcgc ctaccgagtg atctggccga aaaaatcgcg gctggcgaag tcattgagcg
     1801 cccttcgagc gttataaaag agttggtaga aaattcaatc gacgccggtg caaccaaggt
     1861 tgtggttgaa atagttgacg gcggcaaaga gtcgatccgc gtgatcgaca atggatctgg
     1921 aatttttcca gaggatttac ccttagccct tgagcgccac gctactagta aaatcactaa
     1981 actcgatgat ttgtggaatt tgtcgtcaat ggggtttcga ggcgaggctc tccccagcgt
     2041 ctcagcgatt tcgagattta ctattgagag tcgcgtcgcc ggccaagtgg ggcggaaact
     2101 ttacttagag gggggcattt taaaatctga tcaaccgctc ggtgccacca gcgccatcgc
     2161 aagcagcgga actagcctga cggttgaaaa tcttttttat aacgtgcccg cgcgtctaaa
     2221 atttttaaaa gccaaaagtg gggagacctc tcaacttcgc gatctaattg aacgcattgc
     2281 gctttctcac ccgggcgtcc attttacgtt ttctagcgat ggtagaaaaa gcctcgacct
     2341 tccgacttgt tcttcggaag cagcccgtgt tgcccaggtt tttggcacta gcgaaaacga
     2401 cctcgaacat ttctctactc aatttgaagg ctctcaggtt tggggttatc tcgaccgtaa
     2461 tgcccgcatg cctaatagtc gttccgtgta tctttcggta aaccaccgca tggtgcgcga
     2521 taagttgtta cagcaagcag tcttgcttgc cttgcgccct cgtatgatgg aaggcgaata
     2581 tccaaaaatt ttcgtgcaag tactcgttcc cccgaatcaa gtagatgtga acgttcaccc
     2641 tgccaaaagc gaggtgcggt tcgaacggtc gcgcgatgtt tttcaactag tacatggcgt
     2701 aatcgaacgc tctggcagag aaacgaactt agctttttat gcctctccgg atcgttctta
     2761 tggttggaaa gaaccttcaa aaattaacca tgccttttct acagaaaaac taacaccgca
     2821 gccagtggct gcggctgcaa tgccgctcct tgcgggtgac cctgctgctg cacgtaccgt
     2881 ttttcggaca aaaacagata ttggctttga ttcggtgcca cttgcaaaca gcgcaagaga
     2941 gcaaacctca ccgttttctc gacttcagta tattgggcaa ctaaagaata cttatctgct
     3001 ttgccaagac gaaactggat tggtgctcat cgatcaacac gcagcagacg aaagagtgca
     3061 gtacgagcgt ttaaaaaaag aactctcaga aagtgggctt aaatcgcaac cattgctcat
     3121 ttccgccacc cacaaatgct ccgacgaaga tgccgccctc gctattgatt acggagactt
     3181 atttgccaga ctcggcttag aaatcgaatc gtttggcacc aattgtatat taattcgcac
     3241 tgtccccgaa atgttgagcc agcaacatgc ccttgaactt tttcaggcca tgttggcgga
     3301 attgaagtca ctcgaaggcg cctctagcgt ttcgttagta aacgacccta ctcgacattc
     3361 agcgaaactc gaacgattga tcgcaaccat ggcttgtcac tcagcggttc gcgcgggtca
     3421 agccttgtcg gaacgagagg ctcgcgcgtt gatggccacc atggatcgca cggacttcgc
     3481 actaaattgc ccacatggac gccctgcttc gattcaactt tcatactctc aagtcgaagg
     3541 actttttaaa cgttaaaaat gaaaacgata ctttgtattg tcggcccaac ggctagcggt
     3601 aaaagcgaat tggcacttcg gctagccgaa gagttcgacg ccgaaatctt aagctgcgac
     3661 tctttgctgg tttataaaga tttgcaaatt gggactgcca agcctaccga cgacgaaatg
     3721 cgcagagtgc cacatcacgg tgtaaattta gtcgacgcca acgaaaacta taatgcgggc
     3781 gattacgtac gttacgcccg cgggattatc gacgatgcgg tcgcacgaaa aaagaagata
     3841 atcattgttg gcggtacagg cttttacctc aaggcgctac tttgcggcac ctgggacgcc
     3901 ccccccaccc agccggacct gcgggctcag ttcgaaccgc aagattccca actactttac
     3961 gcacagttgc tcgaaaaaga ccccagttat gccgctaaaa ttcaagctaa tgatcgatat
     4021 cgagtgattc gggcactcga aattattgaa gtcacgggca tgcgcctttc cgatcagctt
     4081 gcacaaaaga agccccagaa tccattgcct tatccgtcgc gcatactggg aattcgtaga
     4141 tctaaaccgg acctagaaaa gcggatcatt gcccgaacta acgatatgtt ttcccgagga
     4201 ctcgtgaatg aaaccaaaac gctagttgag cgattttcta catcccccag cgcgcttc
//
LOCUS       DAOMXO010000126         4255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11214_length_4255_cov_4.954286, whole genome shotgun sequence.
ACCESSION   DAOMXO010000126 DAOMXO010000000
VERSION     DAOMXO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11214_length_4255_cov_4.954286"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PLH57_03680"
     CDS             complement(<1..1122)
                     /locus_tag="PLH57_03680"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HRK01739.1"
                     /translation="MALEIKQSLKLSQQLVMTPQLQQAIKLLQLNRMELVEVITQELM
                     ENPLLEEVAETIDNPEGLEAGSEEAAAQQNEPEAPAAEATTTETKDDVDWEQYIEDVN
                     NNQSALPAVRLNTEELPSFENILTKTTTLNDHLRWQLSLAQMSEEERNFGELVIGNLN
                     DEGYLIQPLEELAKEMQMELEDALEVLKMMQKFDPIGVCARDLQECLMVQAHFLQPRN
                     EMVEKIIAHHLPDLERRNYQSIARALGIPMDEVIAATKVILELEPKPGRSFVASDNTQ
                     YITPDIYVYKVGNEYVIMLNEDGMPKLRVSGYYKNVLSGAKQPGQSDGEKKSGAITKE
                     YIQDKLRSAVWLIRSIHNRQKTIYKVTESIVKHQRDFFEK"
     gene            complement(1146..1895)
                     /gene="lptB"
                     /locus_tag="PLH57_03685"
     CDS             complement(1146..1895)
                     /gene="lptB"
                     /locus_tag="PLH57_03685"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRK01740.1"
                     /translation="MSEMNRNRKDRTLEAKKLVKTFGKRTVVREVDFNVSSGQVVGLL
                     GPNGAGKTTSFYMVVGLIRPDGGSVLLDGQDISKLRMHERALQGLGYLPQEPSVFRKL
                     SVEDNLLAVLENTDLTAAQRRETLSNLLEDFNIRHVAKQPAFTLSGGERRRVEVARAL
                     ALDPVFLLLDEPFAGVDPLAVADIQEIIRKLKARGLGILITDHNVRETLGICDLGYIL
                     SDGMILEYGTPEHIAQSPRAREAYLGENFRL"
     gene            complement(1911..2966)
                     /gene="lptC"
                     /locus_tag="PLH57_03690"
     CDS             complement(1911..2966)
                     /gene="lptC"
                     /locus_tag="PLH57_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRK01741.1"
                     /translation="MKRPNFLILALICLFVFVEVLFLRPSNLESNEQDPQGMFNSIES
                     QVLSEKKEDSVAYTIEGFHYTAVEGEQKQWEMDSKQALLYEKSRLARAYNATITMFDV
                     KGKSTLIEGDEAFFRFGSQDLDLVGNVKVTFPDGFWIKTSKAYYRRAIDEVSTTEPFH
                     GEAQPNAGELMHMWGRGFRAKRSEAVVYVLNESRVNMRRLLSDELTDVRSDFAEVDRF
                     KKVAYFTMSSPQKFVETDRGTLHVKSRRQDATYDSKSSSIKVMTAYDDVLIEERDLKK
                     SKKGLKYATCQKADFLTLEDKILLSGFPSVYQEKDTLTGELITIFRKRNVVEVNQANA
                     LHETGSSETVETPAPKR"
     gene            complement(2963..3508)
                     /locus_tag="PLH57_03695"
     CDS             complement(2963..3508)
                     /locus_tag="PLH57_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRK01742.1"
                     /translation="MKSTKPRKFKNPFKQLPSSVQKRLKKIKLLALDIDGVMTDGRVF
                     WVEGTGWTRQFSVKDGYGIKLIMKAGIDVAIISGGDSKSVRERANWLGIKHVYLGDEN
                     KIIAFDKIKKDTGLSSDQIAFVGDDLFDIPVLERVGFSATVPHAVPEVCAIVDYITRD
                     IGGYGAVREICDLIRHAVESR"
     gene            complement(3580..>4255)
                     /locus_tag="PLH57_03700"
     CDS             complement(3580..>4255)
                     /locus_tag="PLH57_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01743.1"
                     /translation="LYASISKEKNPRHMDFLCLWAPTLDSREELGELLKLGAYEAIFT
                     SREEGSVYRYQKNTNGIFHRVEIRSVLWPTSKNWSTDIERAIWDRAKEDSAGRLILDA
                     TDLFTLALRQFAVDSLLGGAAPLLDMLRILKKYDDNIDWQRLRQYKNQLGKCEWMWAG
                     LYAIDLFEKKTGVGLRLPTWARENLVGMRESFWPTFVDSRLVWANPDTRLGEFARAHV
                     KLARGT"
BASE COUNT         1054 a         1068 c          920 g         1213 t
ORIGIN      
        1 cttctcgaaa aaatcacgct ggtgcttaac aatactttcg gtcactttgt agattgtctt
       61 ttgccgattg tgaatgctgc gaatcaacca caccgccgag cgtagtttat cttgaatgta
      121 ttccttggtg atcgcgccag atttcttttc accgtcgctc tgaccaggtt gtttggcgcc
      181 gcttaaaacg ttcttataat aacccgacac acgtaacttc ggcatgccgt cttcgttgag
      241 catgatcacg tattcgttgc caactttgta cacgtaaata tcaggcgtaa tgtactgtgt
      301 gttgtcactt gctacaaaag aacgaccggg tttgggctcc aactccagaa taactttggt
      361 agcggcaatt acttcgtcca tcggaatgcc aagcgctctc gctatacttt gataatttct
      421 acgttctagg tcgggcaagt gatgggctat gattttttcg accatctcgt ttctgggttg
      481 cagaaagtgt gcttgcacca tgagacattc ttgaagatct ctggcacaaa ccccaatcgg
      541 atcaaatttt tgcatcattt ttagaacttc gagcgcatcc tcgagttcca tttgcatttc
      601 tttagcaagt tcttcgagag gctgaatgag atacccctcg tcgttgagat ttccaattac
      661 cagttcacca aagttacgtt cctcttcact catttgagct aacgacaact gccagcggag
      721 atggtcattg agagtagtgg ttttggtgag gatgttttcg aaacttggta gctcttccgt
      781 gtttagtctt actgccggga gcgcactctg attgttgtta acgtcctcaa tgtactgttc
      841 ccaatcgacg tcgtctttgg tttcagtagt ggtagcttct gctgctgggg cctcgggctc
      901 attttgttga gccgcggctt cttcggaacc cgcctcaagc ccctcgggat tatcaatggt
      961 ctcagctact tcctcgagta gcggattttc catgagttcc tgcgtgatga cttccaccaa
     1021 ctccatgcga ttaagctgca atagcttaat tgcctgttgg agctgggggg tcatgaccag
     1081 ctgctgactc agtttcagag actgtttaat ctctaatgcc atggttctcc ggtgtagatc
     1141 tcgtcttaaa gcctaaaatt ttcacctaga tacgcttcgc gagcccgggg gctctgagca
     1201 atgtgttcag gtgtgccgta ctctaagatc ataccatcag acagaatgta gcccaaatca
     1261 caaattccca aagtttctcg aacattatga tctgtgatta aaatgcctag tccacgcgct
     1321 tttaacttac gaataatctc ctgaatatcc gccaccgcga gcggatcgac acccgcaaaa
     1381 ggctcatcta ataataaaaa cactgggtct aacgccaaag cccgcgccac ttcgacgcgc
     1441 cgccgctcac cgccagacaa cgtaaacgcg ggctgcttgg ctacatgacg aatattgaag
     1501 tcctcgagta gattcgaaag tgtttcgcgc cgttgggcag cggtgaggtc tgtattttcg
     1561 agcacagcaa gcaggttatc ttcgacactc agcttccgaa atacgctggg ctcttgcggt
     1621 aggtagccta acccctggag ggctcgttcg tgcatacgca attttgatat atcttgcccg
     1681 tcgagcaaca cgctaccgcc gtctggtcga atcaacccga ctaccatata aaacgaagtg
     1741 gtcttaccgg caccattagg acccaaaagg cccacgactt gtccggaaga aacattgaag
     1801 tccacttccc gaaccacggt gcgtttacca aaagtcttca ccaacttttt ggcttcgagt
     1861 gtgcggtctt ttcgatttcg attcatttca ctcataacta gacttttagc ctaccgtttc
     1921 ggggccggag tttccactgt ttcggatgaa ccggtttcat gaagggcatt cgcctgattt
     1981 acttctacca cattgcgttt tcgaaaaatt gtgatcaatt ccccggttag ggtgtctttc
     2041 tcttgataca ccgacggaaa ccccgaaagt aatattttgt cttctaaagt tagaaaatcc
     2101 gccttttgac aagtcgcgta tttcaaacct ttcttgctct tttttaaatc tctttcttcg
     2161 atcaagacgt cgtcataagc ggtcatcact ttaatgctgc tacttttgga atcgtacgtt
     2221 gcgtcttgcc tgcgagactt tacatgcaat gtaccccgat ccgtttctac aaatttttga
     2281 ggagaagaca tggtgaaata agcgactttt ttaaatctat ctacttctgc aaaatccgaa
     2341 cgtacgtcgg taagctcatc cgatagcaat cgccgcatat tgacccgcga ctcgttcaat
     2401 acgtagacta ccgcctcgga acgttttgcc ctaaacccac ggccccacat gtgcattaat
     2461 tcaccggcat tgggttgcgc ttcaccatga aatggttcag ttgtactcac ttcatcaatt
     2521 gcccgacgat agtaagcctt agatgtttta atccagaacc cgtcgggaaa ggtgactttt
     2581 acgtttccga ctaaatcgag gtcctgtgac ccaaaccgaa aaaaagcctc gtcgccttca
     2641 attaaggtag attttccctt tacgtcgaac atcgtgatgg tggcgttgta ggcgcgcgct
     2701 aaacgggatt tttcgtacaa aagcgcttgt ttcgagtcca tctcccactg tttttgttcc
     2761 ccctcgaccg cggtgtagtg gaatccctcg atcgtgtagg ctacactgtc ttcttttttt
     2821 tccgataaaa cttgtgattc gatagaattg aacatgccct gagggtcttg ttcgttcgat
     2881 tctaaatttg agggtcgtaa aaacagcact tcgacgaata cgaaaaggca aatgagcgct
     2941 agaataagaa agtttggccg tttcatctac tttcaacagc gtgccgaatt aaatcgcaga
     3001 tctcgcgaac ggcgccgtaa ccgccgatat cacgggtaat gtaatccaca atggcacaaa
     3061 cctccggaac ggcgtgaggc acagtggcag aaaagccaac gcgctccaaa actgggatgt
     3121 cgaataaatc gtcaccgacg aatgctattt gatcagacga aagaccagtg tctttcttga
     3181 tcttatcaaa tgcgataatc ttgttttcgt ctcccagata gacatgtttg ataccgagcc
     3241 agtttgctcg ttcccgtact gattttgaat cgccaccact gatgatagct acatcaattc
     3301 cggctttcat gatcagctta attccatagc catctttgac gctaaattgt ctagtccaac
     3361 cggtaccttc gacccaaaaa acacgaccgt cagtcattac tccgtcgata tccaacgcaa
     3421 gtaatttaat tttctttaag cgcttttgaa ccgacgaagg aagctgctta aagggatttt
     3481 tgaattttcg aggtttggtg ctcttcattg gcttttagtg tcttcggttg accgctattt
     3541 gaaaagtcca ttttcgcgct tctcaataaa tcagcgtcat caagtgccac gagctagctt
     3601 aacgtgagca cgagcaaatt caccgagtct tgtgtcagga ttcgcccaaa caaggcgaga
     3661 gtctacgaaa gtaggccaaa acgactcacg catgccgaca aggttttcgc gagcccaggt
     3721 gggcaaacgc aagccgacac cagtcttctt ttcgaataag tcgatcgcgt ataacccggc
     3781 ccacatccac tcacatttac caagttgatt cttatactgg cgaagacgtt gccaatcgat
     3841 attgtcgtcg tattttttta gtatgcgcag catgtcgagc agcggtgccg ccccgcctaa
     3901 aagcgaatca actgcaaact gccgcaaagc tagggtaaaa aggtcagttg catcgagtat
     3961 taatcgtccc gccgaatctt ctttggcacg atcccaaata gcacgctcaa tgtcggtcga
     4021 ccaatttttc gatgtcggcc aaagtacact tcgaatttcc actcgatgaa aaatgccgtt
     4081 agtgtttttt tgataacggt acaccgaacc ttcttcgcgc gaagtaaaaa ttgcttcgta
     4141 agcccctaat ttaagcagtt cgccgagttc ttctcgagaa tcgagcgtgg gagcccacaa
     4201 acaaagaaaa tccatgtgac gcggattttt ttctttagaa atacttgcgt aaagc
//
LOCUS       DAOMXO010000127         4229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11312_length_4229_cov_4.592956, whole genome shotgun sequence.
ACCESSION   DAOMXO010000127 DAOMXO010000000
VERSION     DAOMXO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11312_length_4229_cov_4.592956"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..890
                     /locus_tag="PLH57_03705"
     CDS             66..890
                     /locus_tag="PLH57_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01744.1"
                     /translation="MKKFKCGLWVLVLSLCGANLTWSAPPSLENPTSLNMRRDALLVT
                     VVRGKLKKEIWRNQVLNSLPAEDRESAEALELAIGYWIAAAALDLEAQSRNFFAHDVP
                     TSRSLYWSRLCDLCRYGPFCRLDQVTWDSEVDTRLSTGQIFRKTDPKLFSFYQRFDRQ
                     GPAWEHWPTLRFDVMTDDFLDRCDADDTSLLERASIVAVRASQLKAWSLIAQESGKYA
                     YYRTIKRKLLRPADLVWTSSGERAGGRFVEGDKGWKSLIGANGEVFNQIVTLIPLW"
     gene            complement(1203..2975)
                     /locus_tag="PLH57_03710"
     CDS             complement(1203..2975)
                     /locus_tag="PLH57_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01745.1"
                     /translation="MFRQSFLLTPILSAALAVFGGSAFGTTCRFVIESQNETSGAVVD
                     TISEDPIRFIEMARSRAAEAKAQGKTDFEVPELSTVTKEILVSIANDLDTSPPLADQL
                     IALQIRGEKILANHVPYRELVRLVFQWSETVDTINRVQVLTRLGTARAKTLIEKAATI
                     SHYEFARAAQEILFDLAIETLEELKDTRPIEAQRLIEIGHAQARGDDTAMKAAQWVEA
                     SVKLAATTDRRFPFAPNEFLTLFHESYGRARLEELLEEAPNVLMVFMFGTTSIRTINR
                     LRASGVLVHGLSRHPQYIDNFYLSPVEILFHDVGHSWLMKSQDRRYGLASTPERWLQY
                     LNLERRIDHLAQHDELLAKVAEYYLFEVDHERGYPFDVFINTNEINTEMWVDTILKKL
                     QTKFWAKNPVPDSTFGRLNEARLWLLEQFRELLEEAIERRFQAVKDQPHRVAVVPLQR
                     SERGLPVGGVIAKSGTIYIEAISNKGETFLSDIFNFSVSQVPAEIDGLLDQPETLTLI
                     RKHLDRFMPMKDGERHLTSYELFKMRQLRHAEKKHTPLPFSYRGVGTTYQERGLAIEG
                     APGQKMLVLESGRRVPLHLVCFLP"
     gene            complement(3393..4148)
                     /locus_tag="PLH57_03715"
     CDS             complement(3393..4148)
                     /locus_tag="PLH57_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01746.1"
                     /translation="MRFFFLLLLCFVTTLPYGFSAGNPPTEPNCRGVVGSNEQEFDLQ
                     KALDAHYLQYRGVTSTVVRNSKAFVFVTDQVVADHLPKTHLGHEVEIAVVPVDLKIRS
                     SAGQLTTDFTKAVAAANALGEKFGNEPWCMGIGVEGESDAFLVNFRVSDLEAVPYVLG
                     EWEGVRVDSEISEVADQDHPARIELVRERAESALVALQEKISNSDWYINSQLKEVNGG
                     YVLSVFVTDFQAADQDRTPFAIEDIQILYSKPE"
BASE COUNT         1004 a          959 c         1176 g         1090 t
ORIGIN      
        1 gggctggttt ctgccgcaag gcggtttgct tttttttctg agcttgtcat agagttttcc
       61 ccaaaatgaa aaaatttaaa tgtgggctat gggtccttgt tttgagtctt tgcggtgcga
      121 acctaacatg gagtgcacct ccgagtcttg aaaatccgac ctccttgaac atgaggcgcg
      181 atgcgctgtt ggtgactgtt gttcgaggaa aattgaagaa agaaatttgg cgcaaccaag
      241 ttttaaattc cttgccggcc gaggaccgcg aaagtgccga agcgttagaa ttggcgatcg
      301 gttattggat cgccgctgct gcactcgacc ttgaagccca aagtaggaat tttttcgcgc
      361 acgatgttcc tacttcgcgc agtctctact ggtcgaggtt gtgtgacctt tgccgatacg
      421 gtccattttg taggctggat caagtgacct gggattccga agtagatacg cggctctcta
      481 ccggtcaaat ttttcgcaag accgacccca aactttttag cttttatcaa agatttgatc
      541 gacaaggtcc tgcttgggag cattggccta ctctgcggtt tgacgtgatg accgatgatt
      601 tcttagatcg ttgcgatgcg gacgatacct cgttgctcga gcgagcttcg attgttgcgg
      661 tgcgagcaag tcagctcaaa gcctggtcgt tgatcgcaca agagtcggga aaatatgcgt
      721 actatcgcac gatcaaaaga aaactactgc gcccagcaga tctcgtctgg actagtagcg
      781 gtgaacgggc cggtggacgg ttcgttgaag gtgataaagg ttggaagtcc ctgattggag
      841 caaacgggga agtcttcaat caaattgtca ctttgattcc actctggtga caaactgcta
      901 ccggagtcct tttgcactcg gcgagaaagt tctattgagt cgagcaattg gccaaagtta
      961 acgctgctgg tcattttaaa atcacgaaat acttcagcga gttgcggcag ttcaaaaaca
     1021 gcgtgaccgg agaatgaaag tgtactcatt aagtggtacg ctgtttgctt tactgtattg
     1081 gcgtgtgggt tgggggaact ccaaaaacgg gatggagagc ctcctggtta atagaagcac
     1141 tgacaaagaa gaagaaaaaa aggggctgga gatcagggac gatcttcagc ccttttctct
     1201 ttttatggca gaaagcaaac caaatgcagc ggcacccgcc gccccgattc gagtactagc
     1261 attttttggc caggagcgcc ttcaatcgca agtccgcgtt cttgataggt ggtacccacc
     1321 ccgcgataag aaaacggcag cggtgtatgt tttttttcgg catggcgtag ttggcgcatt
     1381 ttgaaaagtt cgtacgaagt caggtgcctc tcgccatctt tcatcggcat aaagcggtcc
     1441 aggtgtttac gaatcagtgt gagcgtttcc ggctgatcga gcaacccatc tatttcggct
     1501 ggcacctgag aaaccgaaaa attaaaaatg tcgctgagaa aagtttcacc cttattagaa
     1561 atggcttcga tataaatcgt gccactctta gcgatcactc cgccgaccgg taacccccgt
     1621 tcgcttcgtt ggagaggtac cacagctacg cggtgaggtt gatctttgac agcttggaag
     1681 cggcgttcga tggcttcttc gagcagttcg cgaaactgtt cgagcaacca aagtcgggct
     1741 tcgttcaacc ggccaaaggt cgaatccggc accgggtttt tggcccaaaa cttggtttgg
     1801 agttttttga gaatcgtatc gacccacatt tcggtgttga tctcgttggt gttgatgaac
     1861 acatcgaagg ggtagccccg ttcgtggtcg acttcgaaga gataatattc cgccaccttg
     1921 gctaaaagtt cgtcgtgttg ggcgaggtga tcaatgcgcc tttcgaggtt taggtattgg
     1981 agccagcgtt cgggggtaga cgctaaaccg tagcggcgat cttggctttt catcagccag
     2041 ctgtggccca cgtcgtgaaa aagaatttcg acgggagaaa ggtagaagtt atcgatgtat
     2101 tgggggtggc gcgaaagtcc atgcaccagt actccagagg cacgcaagcg attgatcgtg
     2161 cgaatggagg tcgtcccaaa cataaagacc attaaaacgt tcggggcttc ttcgagcagc
     2221 tcttccaggc gagcgcggcc gtagctttcg tggaaaaggg tgagaaattc gttgggcgcg
     2281 aatggaaacc ggcgatcggt ggtggctgcc aacttcacgc tggcttcgac ccattgagcg
     2341 gctttcatgg cggtgtcgtc cccgcgggct tgggcgtggc cgatttcgat caaacgttgg
     2401 gcttcgattg gtcgtgtgtc tttaagctct tcgagcgttt cgatggcgag atcaaaaaga
     2461 atctcttgag ccgcacgggc gaattcgtaa tgcgaaatag ttgctgcttt ttcgatgagt
     2521 gttttagcac gcgcggtgcc cagacgggtc agtacttgca cgcgattgat cgtgtcgacg
     2581 gtttcggacc actgaaacac caatcgaacg agttcgcggt aaggtacgtg gttcgctaaa
     2641 attttttcgc cgcgaatttg cagcgcgatg agttggtcgg cgaggggtgg cgaagtgtcc
     2701 aggtcgtttg cgatggaaac gagaatttct ttggttacag tggataattc aggaacttca
     2761 aaatcagttt taccctgtgc cttagcttcg gccgcccgcg aacgcgccat ttctataaat
     2821 cgaatcgggt cttcgctaat cgtgtccaca acggcgccag aggtttcatt ttgtgattca
     2881 atgacgaacc ggcaggtggt gccaaaggca gaaccaccaa aaaccgccag ggcggccgag
     2941 agaatggggg tgagaaggaa gctctggcga aacatctgcg ttcttatata gagctgtatt
     3001 ttttaagtca agtattgaga tttggtcagg gaaatagacc gaagactcag atcctggtcc
     3061 ggaaatgtgg atacccgtgc cacgttagtt gcgtttgcaa tggcttcgtt tttgctgaag
     3121 tgtttcccgc caaactcagg cgagtagatg gatctacctc cttttcgtaa ggaaggaggt
     3181 gatcccatat gaaaaatacc accaggtatt taacgtatcg gattcggcta gaaaaactta
     3241 agcaaaagac ctggttgctc atagtcttag ccaaactttt gccttttcta gcgctgttgc
     3301 attccaacta tgtaggacaa acgtgaggtg ggcttcgggt acagcttgac gccaaaaccg
     3361 gagtctctca cgaagagatc gactgggttt ggttattctg gttttgagta taggatttga
     3421 atgtcctcga tcgcgaacgg agtgcgatct tggtctgctg cttggaaatc agtgacaaaa
     3481 acgctgagta cgtaaccgcc gttcacttct ttgagttgac tattgatgta ccaatcagaa
     3541 ttcgaaattt tttcttgaag tgcaacgagc gccgattctg cgcgttcgcg aaccagttcg
     3601 attctggcag ggtggtcctg gtcggcgact tccgaaattt cggaatcaac gcgaacgcct
     3661 tcccattctc caaggacgta ggggacggcc tcgaggtcgg acactctaaa gtttaccagg
     3721 aaagcgtcgc tttcgccttc gacgcctatg cccatgcacc acggttcatt gccaaatttt
     3781 tctcctagcg cgtttgccgc cgcgacggct ttggtgaagt cagtggtgag ttgtccggcg
     3841 gaagaacgaa ttttaagatc gacgggaacg actgcgattt cgacctcgtg ccccaaatga
     3901 gtttttggca aatggtctgc gaccacttgg tcggtcacga aaacaaaagc cttggaattg
     3961 cgaaccacgg tgctcgtgac tccgcggtat tgcaggtagt gggcatcgag ggctttttgg
     4021 agatcaaact cttgttcatt agagccgacg accccgcggc agttcggttc tgtgggcgga
     4081 ttgccagccg agaaaccata gggaagggtg gtaacaaaac aaaggagcag taagaaaaaa
     4141 aagcgcatga gccgagagct acattaagtc ccagtgcagt ttcaagcgtc gaatcgaacg
     4201 gcgaattttg gcctttgccg tggcctgtt
//
LOCUS       DAOMXO010000128         4214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11368_length_4214_cov_4.497475, whole genome shotgun sequence.
ACCESSION   DAOMXO010000128 DAOMXO010000000
VERSION     DAOMXO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11368_length_4214_cov_4.497475"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..348)
                     /gene="rnpA"
                     /locus_tag="PLH57_03720"
     CDS             complement(7..348)
                     /gene="rnpA"
                     /locus_tag="PLH57_03720"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRK01747.1"
                     /translation="MKKKNSFARSERLTQRKEFIRLFDEPGVHRGGTFLAFFKDSETG
                     RSRLGITIKGKVSSVWRFRIKRTIREWFRLHKDMLGNIDLNIVVKPPVNLVKSYIDRL
                     KKDLTQWRGGV"
     gene            complement(372..521)
                     /gene="rpmH"
                     /locus_tag="PLH57_03725"
     CDS             complement(372..521)
                     /gene="rpmH"
                     /locus_tag="PLH57_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRK01748.1"
                     /translation="MKRTLQPSRKKRRRTHGFLARMSTSNGAKVLNRRRRKGRKRLAP
                     TTSRK"
     gene            640..2202
                     /gene="dnaA"
                     /locus_tag="PLH57_03730"
     CDS             640..2202
                     /gene="dnaA"
                     /locus_tag="PLH57_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRK01749.1"
                     /translation="MSSHSFNWEQLLSEVGKKIPPAQFRAWLDPVRFLELTSESLVVT
                     APTPFSKDWITDNYKTIFEEALLSLYGVHASLKVEVDSEMGLISTTDVNAENDSNHIV
                     KSGEGTKHLHEESELSSEIETPQSNEASTNPGLPLFEYIKSSKTSEPRIHTRYQEERP
                     ASNTPVAAISVRDLTTPVVRTQGQSLDPKQTFDRFVVGASNQFAHAAARAVAEQPASQ
                     YNPLFIYSGAGLGKTHLLHAIGNYVLKVNPKAKICYLSAEKFVNDLIDSIRHEKMSQF
                     RSKYRDGFDMLLMDDIQFIAGKDRTQEEFFHTFNALHSSKRQIVVTSDKFPKDIPGLE
                     ERLRTRFEWGLVADIQPPEIETRIAILKAKAEIDDIFLPNDVAVFLASHIKTNVRELE
                     GALIRMGAQASLSGVEISLELAKRELKTIIQEPSLLITPDVILDAVASHFSIRLADLK
                     GSVRSRKVALPRQIGMFLIRKYCTHSFTEIGLLFGGKDHSTVMHGVRSVEKSLLSDAE
                     IRGHVESIQNSL"
     gene            2866..3975
                     /gene="dnaN"
                     /locus_tag="PLH57_03735"
     CDS             2866..3975
                     /gene="dnaN"
                     /locus_tag="PLH57_03735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRK01750.1"
                     /translation="MKFTLQKDSFLEALQKVQNVVERKNTVQILSNVLLTTDKDSLSL
                     TATDLEVGIVVNQPVDKSSEGKVAISAKNLVEIVKELPNKAVTLTKKDNNWVEISCQK
                     STFNLVGLSAEEFPPLPAFESKSFLKVNAAAAREMIDRTLFAVSTDETRYHLNGVFFE
                     TIENGLIRMVATDGHRLSFIDRELFLSKTDQFERGIIIPKKGLGELRRLIEGKVDEFS
                     LALDKGNLLVKLDRTLLFIRLIEGDYPDYEQVMPKKADRRLTVDREEFLGSLRRVSLL
                     ANEKSRGVKLALSANNLVITSSNSDLGDAKEELDVDYSAEQVEIGFNARYVIECLSIL
                     DCEKVTFDLNDKLSPGLLRPAGRSDYTYIVMPMRI"
     gene            4005..>4214
                     /locus_tag="PLH57_03740"
     CDS             4005..>4214
                     /locus_tag="PLH57_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK01751.1"
                     /translation="MYIESLRLYQFRCFEELTLEPKRDFNVISGLNGQGKTSLLEAVG
                     LLGSLKSFRHAKNLELVQFEKLEALV"
BASE COUNT         1187 a          961 c          909 g         1157 t
ORIGIN      
        1 tatctttcat actccgcccc gccattgagt tagatctttc ttgagccgat cgatgtagct
       61 tttaacaaga ttcacagggg gttttacaac tatatttaag tcgatatttc cgagcatgtc
      121 cttgtgcaaa cgaaaccatt ctcgaattgt tcttttgatt cggaaacgcc acacagagga
      181 aaccttgccc ttaatggtaa tacctaaacg tgaccggccg gtttcactgt ctttgaaaaa
      241 agccagaaac gtaccaccgc gatgcacacc cggttcatca aacaaccgaa taaattcttt
      301 tcgttgagtg aggcgttccg acctggcaaa tgaattcttt tttttcaccg acaagcgagc
      361 gtcgcgcaaa tttatttacg ggaagtagta ggcgctaagc gcttgcgacc ttttcggcgg
      421 cgacggttta acaccttcgc tccattcgac gtgctcatgc gcgccaaaaa gccgtgcgta
      481 cggcgacgtt tcttccgact tggttgaagt gttcttttca tagtgctgcg ttcatacgac
      541 gctagaccgt ccgtgtcaag aactcgcgcg atactgtatc gcagcttgcc caagaacacc
      601 tcttctgtta tccctctctt cctcgtcaat cgggggatta tgtctagcca ttcattcaac
      661 tgggaacagc tactttcgga agttggcaaa aaaattccac ccgcacagtt tcgtgcatgg
      721 ctggatccag tgcgttttct cgagctaacc tcagagagcc tagtggttac ggcgcccacg
      781 ccttttagca aagactggat aactgacaat tataagacca tttttgagga ggcgctgctc
      841 agcctttacg gggtacatgc ctcattaaag gtggaagtcg actccgaaat gggcctcatt
      901 tcaacgaccg acgttaatgc agaaaacgat tcgaatcata tagttaagtc cggtgaaggg
      961 acaaaacatt tgcatgaaga aagtgagttg agttccgaaa tcgaaacacc acagtcgaat
     1021 gaagcgtcaa cgaatccggg actccctctt tttgaatata taaagtcgtc gaagaccagc
     1081 gaacctcgaa ttcacacccg ttaccaagag gaacgccccg caagcaatac cccggtagca
     1141 gcgatctcag taagagacct tacaaccccg gtcgttagaa cccagggtca atcgcttgac
     1201 ccgaaacaga cgttcgatcg gtttgtagtt ggtgcaagta accagtttgc ccacgcggca
     1261 gcgcgagcag tggcagaaca gcccgccagc cagtacaatc ctctattcat atatagcgga
     1321 gcgggccttg gtaaaacgca cctactccat gcaattggta attacgttct caaagtgaac
     1381 ccgaaagcta agatttgtta tctttcggcc gaaaagtttg taaacgatct cattgattcg
     1441 attcgtcacg aaaaaatgag ccagtttcgt tctaaatacc gcgacggatt cgacatgctg
     1501 ttgatggacg atattcaatt catcgctggc aaagaccgta ctcaggaaga attttttcac
     1561 actttcaacg cacttcattc ttcaaaaaga caaattgttg ttacgagcga taagtttcct
     1621 aaagatatcc cagggctaga agagcgacta cgtactagat ttgaatgggg tttagtggcg
     1681 gatattcaac cgcccgaaat tgaaacacga atcgctatcc tcaaagctaa agcagaaata
     1741 gatgatattt tcttaccaaa cgatgtcgcc gtatttttgg ctagtcacat taagacaaat
     1801 gtacgggaac ttgaaggcgc gttgattcgc atgggtgcac aagcttcgct ttccggtgtt
     1861 gaaattagtc ttgaactcgc aaaacgggaa cttaagacta ttatccagga accttcgctt
     1921 ttaattaccc cagacgtaat cctggacgcc gtggcttctc atttttctat tcgattggcc
     1981 gacttaaagg ggtcggttcg atctcgaaaa gtggctcttc ctcgtcaaat tggtatgttt
     2041 ttaattcgaa agtactgcac tcactctttt actgaaatcg gtctcctctt cggtggaaaa
     2101 gaccattcaa ccgttatgca cggcgtaaga tccgtagaaa aatctctttt aagcgacgct
     2161 gaaatccgag gccacgtcga atcgattcaa aattctcttt gaatcaatat ttgcgctttg
     2221 cacacagctt atccacataa cattgcaagt tatccacaag acacccccta cttatccaca
     2281 gccggggtaa tacctgtcca catttcaatt ttgcgtcttt gacttatcca caatgcaccc
     2341 aaaaatctgt ggataaccct gtggataact tgtggataag ttttctgtta aaaagttatc
     2401 cacagagtta tccaccgact tatccacaag gaaatcaaaa taattccaaa taattaactt
     2461 agttatccac attatccaca aggcggaact atccacatgc gaactcaaac tcttaaaagt
     2521 tatccacacc tgtggataac tctcgaaaaa tacccgtagc ttggcgcagc aattattggg
     2581 ttatccacaa cgtgctgcga aaattgtgga taacttttta attacaaaat gttatccaca
     2641 gatccctgtt gaaaacctgt ggataaagtg gtttaaaacc tccgatgagg ggttttccac
     2701 aatttggttc gagttatcca cagagttatc caccgagtta tccacacgga aaattctttt
     2761 ttatcaaggg cttggttgag ttatccacac aatccgcagc cttattacta ctactgtatt
     2821 tatatatatt aattatttag aaggtggaga gttgaggaga acatcatgaa gtttacactt
     2881 caaaaagata gctttcttga agcgcttcaa aaagttcaaa acgtcgttga gcgaaagaac
     2941 actgttcaga ttctttctaa cgttcttctg accactgaca aggattcact ctcactaaca
     3001 gcgaccgatc ttgaagtagg tattgtcgtt aatcaaccag tggataagtc cagtgaaggt
     3061 aaggttgcaa tctcagctaa aaatcttgtg gagattgtga aagagctccc aaataaagct
     3121 gttacactca ccaaaaaaga taacaactgg gttgagatat cctgtcagaa gtccactttt
     3181 aatcttgtgg gtttaagtgc agaagagttt ccaccacttc cagcttttga aagtaaatcc
     3241 tttctaaaag taaatgcagc ggctgctcgt gaaatgattg atcgcacgct atttgctgtt
     3301 tcgaccgacg aaactcgtta tcacctcaac ggggtgtttt tcgaaacaat tgaaaacggc
     3361 ctcattcgta tggtggctac cgatggccac cgcctttcgt ttatcgatcg agaacttttt
     3421 ctatcgaaaa cggaccagtt tgaacgcggc atcataattc ccaaaaaagg gttaggagag
     3481 ttgcgccgct tgatcgaggg taaggtagat gaattctcgc ttgctctaga taaaggcaat
     3541 cttttggtaa aactcgaccg tactttgctc tttattcgcc ttatagaagg tgattaccca
     3601 gattatgagc aagtgatgcc aaagaaggcc gatcgacgtt tgacggtcga ccgtgaagag
     3661 ttcttgggca gtctgcgaag ggtgtctctt ctagctaatg aaaaaagcag aggcgtgaag
     3721 ctggcgctct cggccaataa tctcgttatc acttcaagta attccgactt aggcgatgca
     3781 aaagaagaac ttgatgtgga ttattctgcg gaacaagtcg aaatcggctt taatgctcgg
     3841 tatgtaattg aatgcctatc catcttggat tgcgaaaaag tgacgtttga tctcaatgac
     3901 aagttgagtc ctggcctatt gcggccagcc ggtcgttcag attacaccta catagtcatg
     3961 ccgatgcgaa tttagcgtcc aaaatacgaa gatgcgatga cgcgatgtat attgaatcac
     4021 tgcgtcttta tcaatttcgc tgctttgaag agcttacgct cgagccgaag cgcgatttta
     4081 atgtcattag cggactgaat gggcagggta aaacgagtct tttggaggct gtgggccttt
     4141 tgggctcgct caagagcttt cgtcatgcga aaaacctaga gctcgtgcag ttcgaaaaat
     4201 tagaggcctt agtc
//
LOCUS       DAOMXO010000129         4210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11381_length_4210_cov_4.421420, whole genome shotgun sequence.
ACCESSION   DAOMXO010000129 DAOMXO010000000
VERSION     DAOMXO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11381_length_4210_cov_4.421420"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLH57_03745"
     CDS             complement(<1..738)
                     /locus_tag="PLH57_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01752.1"
                     /translation="MKKNLLNQAINLAFVYLIAICCAPNVRAAENTDLNAMFAFKKVF
                     VASPKDNVDGAYVQPVSEAFADIFEKNLRFELVDDIDIADTVVESSIEKKVSGTDLEI
                     KLLLQPSKEVFVVERASLPAASSGGELVNQLKQLLKTALKRIPFYGTVTGRDADMVTL
                     DIGSIQGLLEGDIVQVSRVERVKRHPLLKTIVDVELVPVGSVEITEIEDSVAFGKVHK
                     TLTGEGILRLHKVTAIESRMPKERENKD"
     gene            complement(707..1015)
                     /locus_tag="PLH57_03750"
     CDS             complement(707..1015)
                     /locus_tag="PLH57_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01753.1"
                     /translation="MERWIPIVDYAVLKGISTSTLRRYIKAQKIIYKIENGRYLILSE
                     EAQEKKELNWSPSPSPDTLSVFARVRDLEQQLIQAQEQINELKMLVAIYEEKLAQPGH
                     "
     gene            1092..1382
                     /locus_tag="PLH57_03755"
     CDS             1092..1382
                     /locus_tag="PLH57_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRK01754.1"
                     /translation="MKKKRIAFVCSGGAAKAGAYHLGVALALQEKGFVFSGGLVDDPV
                     EHESKKLIDIYVGSSAGSIISTYLASGYSLENIFDSFLGRKSPDAQGRILRK"
     gene            1395..2303
                     /locus_tag="PLH57_03760"
     CDS             1395..2303
                     /locus_tag="PLH57_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRK01755.1"
                     /translation="MFSLRKDMGLFRTLSELSEGVRMPALARFIQGIAKDSYESLFHW
                     KWLKWSGVFATTGIEKYLREEVLPSNDFKRLRPDLFIVATQLNHSRKVVFGKYKYAVD
                     PLDKTVGYRDDVGISDSCAASTALPLIYAPYPIKSEQGKVVYYFDGEIRDTLSTHVAT
                     DAGADLVIASYTHQPYHFTKEVGSLHEFGMPAILIQALYLMIERKIQSSQAMHTKHKL
                     ALDAVSEFFRDHQLSDELRKKCMKVMEEKLGTRRDVEFVYIHPKSNDREMFFGDHFNL
                     SGKGMTEIVRSGFKSAIDILRRHNLE"
     gene            2296..3528
                     /locus_tag="PLH57_03765"
     CDS             2296..3528
                     /locus_tag="PLH57_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HRK01756.1"
                     /translation="MNKIFAAFTSRRMFVTLLLGFASGLPFALSGATLQAWMADQKVD
                     LAVIGIFSLVALPYSLKFIWAPFMDRYVPPFLGRRRGWMIIAQFALAVVIFLLGQSDP
                     VLNPAKVAFIAVLVAFFSASQDVVIDAYRTEVLPTEEYGIGSGVYVMAYRIAMITSGG
                     GALVLADHMPWSRVYTIMAVLMGLGALGSLIGPEPTLEAKPPKSIKEAVMDPFLEFFK
                     RKGAIETLLFLLLYKLDVFMTLAMQTPFLLEIGFSKTDIGVITKFFGMGSTILGALFG
                     GGLMLRLGMERSLWVFGAAQALAGLSYMLLAHIGANHSLLVFAIGIENLCSGMGIAAY
                     SGFMMSLCDRRFTATQYALLTSFMGLSRSFVQTPSGFLAKAIGWEYYFLVCALIGIPG
                     LLLLTRFKRWYVPGMANK"
     gene            3595..3903
                     /locus_tag="PLH57_03770"
     CDS             3595..3903
                     /locus_tag="PLH57_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HRK01757.1"
                     /translation="MSFSTKEFIDFLKKYQVIGLAIAVIIGGKLNEFVSTLVNDLLVP
                     LIFQPALKAARVDDIRSLSFHGIKYGKVMGAFIDFAIVAFVVYLISKFILREKEVTKK
                     "
     gene            3917..>4210
                     /locus_tag="PLH57_03775"
     CDS             3917..>4210
                     /locus_tag="PLH57_03775"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRK01758.1"
                     /translation="MKFLKIMLIVILAGVMGKTTYASNESKQLIDGFTNESKLGIVIT
                     TGNSKTLTFDAAQKDTYEWGHNKLGFLGTYLRSTSKGIESARRWSLGLRYDRAI"
BASE COUNT         1053 a          865 c         1057 g         1235 t
ORIGIN      
        1 atctttgttt tcgcgctcct tgggcattcg cgactcgatc gccgtaactt tatggagtct
       61 taaaattcct tcgccagtta gggttttgtg aactttgccg aaggctacgg aatcttctat
      121 ttcggtaatt tctactgaac ctacgggcac cagttccacg tctacaatgg ttttaagcaa
      181 aggatgtcgc ttaacccgtt cgactcgcga aacttggaca atatcgccct caagtagccc
      241 ttggatagaa ccaatgtcga gggttaccat atcggcgtcg cgcccagtta cggtgccata
      301 aaacgggatc cgcttgagcg cagtttttaa aagttgcttc agttgattta cgagctctcc
      361 tccgctgctg gcggcgggca acgatgcccg ctccactaca aaaacttcct tggagggttg
      421 cagcaggagc ttgatctcga gatcggttcc gctgactttc ttttcgatag acgactcgac
      481 gacggtgtcg gcgatatcaa tgtcgtcgac tagttcaaag cgtaaattct tttcaaaaat
      541 atctgcaaat gcctcagaaa cgggctgtac gtaggcgccg tcaacattgt ctttgggcga
      601 tgccacgaac acttttttaa atgcgaacat ggcattgaga tcggtgtttt cggctgcacg
      661 aacgtttggc gcgcaacaaa tagcgattaa gtatacaaaa gcgaggttaa tggcctggtt
      721 gagcaagttt ttcttcataa attgcgacga gcattttgag ttcgttgatc tgttcctggg
      781 cctggatgag ttgttgttcc aggtcacgaa cgcgtgcaaa cactgaaagt gtgtctgggc
      841 tcggggaggg gctccagttt agttcttttt tttcttgagc ctcttcggac agaatcaggt
      901 aacgaccatt ttcaatcttg tagataattt tttgggcttt aatgtaacga cgcaatgtcg
      961 aagtgctaat tcccttgagt acagcgtaat cgacgatggg gatccaacgt tccatgagta
     1021 agctcctgcc tagaatttta tttctcgcag tcgcgaatgt aaagtaacat taatgtaggg
     1081 ggttccagcg cttgaagaag aaacgaatcg cgtttgtatg tagtggtgga gccgccaaag
     1141 ctggtgctta ccatttaggg gtggcgttag ccctgcaaga aaagggtttt gtgtttagcg
     1201 ggggattggt cgacgacccc gtggagcacg agtcaaaaaa actaatcgat atttacgtgg
     1261 gttcgagcgc cggaagcatt atttccacct atttggcgag cggttattcg ttagaaaata
     1321 tttttgattc ttttttgggt cggaaaagtc ccgacgcaca aggacgcatt ctgcgaaagt
     1381 gaacctactc aaaaatgttt tcactgcgta aagacatggg cctttttcga accctgagtg
     1441 aactatcaga gggcgtccgc atgcctgcgc ttgcgcggtt tattcagggc atcgccaaag
     1501 attcgtatga gagcctattc cactggaaat ggttaaagtg gtctggcgtt tttgcgacga
     1561 cgggtatcga gaagtattta cgcgaagaag tgttgccgag taacgatttt aaacgactac
     1621 gaccagattt atttatcgtt gcgacacaat taaatcattc gagaaaagta gtgtttggaa
     1681 aatataaata cgctgtcgac ccacttgata aaactgtagg ctatcgcgat gacgttggaa
     1741 tatctgattc ttgcgccgca agcaccgccc tgcctttgat ttacgcgccc tatcccataa
     1801 aaagcgagca gggaaaggtg gtttattatt tcgatggtga aattcgtgac acactttcta
     1861 ctcatgttgc gactgatgca ggagcagatc tcgtgattgc aagttatacg caccagccct
     1921 accactttac caaggaagtt ggatctcttc atgagtttgg catgccagcc attttgatac
     1981 aggctttgta tttaatgatt gaacgaaaaa ttcaaagctc tcaagcgatg cacacgaaac
     2041 ataaattggc gttggatgcg gtttcagagt tcttcagaga tcaccaactg agtgatgaat
     2101 tacgtaaaaa atgtatgaag gtcatggaag aaaagctcgg aacccgtcga gatgtcgaat
     2161 tcgtatatat tcacccgaag tcgaatgacc gcgaaatgtt tttcggagat cactttaact
     2221 tgtcgggaaa aggaatgacc gaaatcgtaa ggtccgggtt taaaagcgcc atcgatattt
     2281 tgaggagaca taatcttgaa taagattttt gcagcgttta ccagcaggcg aatgtttgta
     2341 acgttgctac tcggctttgc ttcgggactg ccgtttgcgc tttctggagc cactcttcag
     2401 gcctggatgg ctgaccaaaa agtagatttg gccgtcattg gaatattctc attggttgct
     2461 ctcccctatt ccttaaaatt tatttgggca ccttttatgg atcgttacgt gccgccgttt
     2521 ttggggcgcc gtcgcggttg gatgatcatt gcgcagttcg cgctagcagt tgtgattttt
     2581 ttactgggtc agtctgatcc ggttttaaat ccagcgaaag tcgcttttat agcggttctg
     2641 gtagcatttt ttagtgcgag ccaagacgta gtgattgatg cctatcgaac agaagtgttg
     2701 cctacggagg aatacggaat aggttcgggc gtctacgtaa tggcgtatcg aattgcgatg
     2761 attacgtcag gcggtggggc gctggtgctc gctgatcaca tgccttggag tcgcgtttac
     2821 actatcatgg ccgttttaat gggtcttggg gcattaggct ccctgattgg gccggaacca
     2881 actcttgaag cgaaaccacc gaagtccata aaagaagccg taatggatcc atttctggag
     2941 tttttcaaaa gaaagggcgc catcgagacc ttgttgttct tgctgctcta caaactcgac
     3001 gtatttatga cgcttgcgat gcagacgccg tttttactcg agattggatt ttccaaaacc
     3061 gacataggag taatcacgaa attctttggt atggggtcta ccatacttgg cgcgctattt
     3121 ggcggtgggc tcatgttgcg ccttggcatg gagcgttcgc tttgggtttt tggtgcagcg
     3181 caagcgttgg cgggactaag ttatatgtta cttgcgcata taggtgccaa tcactcactg
     3241 ttggtttttg ccataggtat tgagaatttg tgcagcggaa tgggaattgc cgcttattcg
     3301 ggttttatga tgagtttatg tgatcgccgt tttacggcta ctcaatacgc gctgttaacc
     3361 agctttatgg gcctttcgcg tagcttcgtt caaaccccat cgggattttt ggcgaaagca
     3421 ataggatggg aatactactt tttagtttgt gctttaattg gaataccagg tctgttgctg
     3481 cttacgcgct ttaagcgttg gtatgtaccc ggtatggcaa ataaatagta acgctcgaaa
     3541 attcatttgg tttgttctat agagggggtc tcgtagtatt ttagggagat ctcaatgtcg
     3601 tttagtacca aagaatttat cgatttttta aaaaaatatc aggtcatagg tttggccatc
     3661 gcagttatta ttggcggaaa actaaatgaa tttgttagca cactagtaaa cgaccttttg
     3721 gtgccgctga ttttccaacc agccttaaag gcagcacgag tcgacgacat tcgatcgttg
     3781 tcgtttcacg ggattaaata cggaaaagtt atgggcgcgt ttattgattt cgcgattgtt
     3841 gcttttgtag tttatttgat ttcgaagttt attcttcgcg aaaaagaagt cacaaaaaaa
     3901 taaggatcag ccgaacatga aattcttgaa aataatgctc atcgttattc tagcgggcgt
     3961 aatgggtaag accacgtatg catccaacga gtcaaagcaa ttgatcgatg gttttaccaa
     4021 cgaaagcaag ttgggcattg tgattacaac cgggaactcc aaaaccctta ctttcgatgc
     4081 ggctcaaaaa gacacctacg aatggggtca caacaaactt ggctttctgg gtacctacct
     4141 tcggtcaact tcgaaaggta ttgagagcgc cagaagatgg agcctcggtt tgcgctatga
     4201 tcgggcaatt
//
LOCUS       DAOMXO010000130         4198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11434_length_4198_cov_5.585325, whole genome shotgun sequence.
ACCESSION   DAOMXO010000130 DAOMXO010000000
VERSION     DAOMXO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11434_length_4198_cov_5.585325"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..737)
                     /locus_tag="PLH57_03780"
     CDS             complement(102..737)
                     /locus_tag="PLH57_03780"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRK01759.1"
                     /translation="MPNPKSSHVEVDVEKLPFQEAYKLIIGSIVPRPIAWVSTVNKSG
                     VSNLAPFSFFNGVCSNPPSVLFCPVNTPEGREKDTLVNIRDNRQFVVNLVSRGLAEKM
                     NQTSANYPFGISEFKEAGLTEGKCKKVKPPRVLEAPISMECELIEIVKVGPGGPGSGA
                     VVIGKILHMHFDPAVYKDGKILIEAFDPIARLAGSAYCPVRDIFTLDRPKL"
     gene            complement(724..1854)
                     /locus_tag="PLH57_03785"
     CDS             complement(724..1854)
                     /locus_tag="PLH57_03785"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRK01760.1"
                     /translation="MSHKPPQIPFSSGTVSKQTHVDLPNGTFEEEYGRQGFFGRTTHL
                     YRKNAPTGWTRIDGPLHPHAYDANAALGTATTAPKRFLGNDDVCLWIYAPQKQTPHYV
                     RNADGDEAWFIHAGSGFLETDFGPLKYRKGDYLVVPRGTTYRFFPDAGAPQFYLLIES
                     FSEFELPDRGSLGRHAFLDPACIDVPQAAASGAPATDGKEWKLKIKRQNEYTDVYYPF
                     NPLNVVGWRGDAFAYRFNVEDIRPIISPRFHLPPSVHTTLMAKNFVLCTFAPRPMETG
                     DPKAQRVPFFHSNIEFDEVIFYHDGDFFSRSGITPGMFTFHPQGIHHGPHPKAFANQF
                     SKEFAQEYAVMIDTTRPLKAFDTAVSCEMVDYKNSWKEYAKP"
     gene            complement(1886..2827)
                     /locus_tag="PLH57_03790"
     CDS             complement(1886..2827)
                     /locus_tag="PLH57_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRK01761.1"
                     /translation="MKLVTFLSKPYRRTDKPNDYAWNRAAQARWGVWIDNDVFDFTSK
                     KVPTLLHLIEAGPSAWKQLGSAVRLKKNKSQRVAGRVPVSRLTLLSPIPRPPSMRDFL
                     AFEEHSSWSWKKRGLDLPPEWYEMPVYYKGNHRTLWGPGDTIRWPSFTQKLDYELELT
                     MVLGRSGVNIKKEKALDHIFGYAIMNDWSARDIQAKEMLCRLGPAKGKDFGTSIGPCI
                     VTKDELGDANGLRMQAFINDQCWSDGNSATRHWSFSDMIAHVSRDEPVFAGDLYGSGT
                     VGRGCGLELDRWLTPGDTVTLKIEGIGELQNRVGAKP"
     gene            complement(2828..3268)
                     /locus_tag="PLH57_03795"
     CDS             complement(2828..3268)
                     /locus_tag="PLH57_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01762.1"
                     /translation="MNFNFVSKVTFVALAIGFAFGCTKNKTTATPGKISGRIVIDQQL
                     VPSLKPTDTLFIIARNQQSGPPTAVKRIVNPTFPLEYVIGPEDAMMGGTGGFEAGAQL
                     TIAARLSRSGDAMPAAGDIEGVHKDNPALVGSGGVDVLIDRVRP"
     gene            3397..3636
                     /locus_tag="PLH57_03800"
     CDS             3397..3636
                     /locus_tag="PLH57_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01763.1"
                     /translation="MGNSLFDDVVRLTGLPEETIFGELSDIVGKVGAEPKSLTIEQLR
                     AAMLCYLEELASFEEFSIDETPQSPVKIIKEDNPS"
     gene            3691..>4198
                     /locus_tag="PLH57_03805"
     CDS             3691..>4198
                     /locus_tag="PLH57_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRK01764.1"
                     /translation="MNRNQALEVLSQYIPNPIVVEQTARGERQYDIYSRLLLDRIVFL
                     GTEVNDTVANLIVAQLFFLESQDPEKDVHLYINSPGGSVSAGLAIYDVMQFIKCDVST
                     YCLGMAASMGSLLLCAGAKGKRYSLPHSRIMIHQPLGGARGQATDIEIQAKEILYLRA
                     RLNEIYSRH"
BASE COUNT         1068 a          991 c         1047 g         1092 t
ORIGIN      
        1 aggtaggaat gaagagcaac aagagaataa aaaaaaccgc atgaatcgcc ttcctagcga
       61 cttacccgcc ttttttcaac tgaatctcct ttaaataatg gctaaagctt cggtcgatct
      121 aacgtaaaaa tatctctcac cggacagtag gccgatccag ccaatcttgc gattgggtcg
      181 aaagcttcga tcaatatttt gccatcttta tagactgcag ggtcgaagtg catgtgtaat
      241 atttttccaa tgaccacagc cccacttccg gggccaccgg ggcccacttt gacaatttca
      301 ataagttcac actccattga aatgggagcc tctaataccc gcggaggttt gacttttttg
      361 catttgccct cggtcagacc ggcttctttg aattcactta tgccgaaggg atagtttgcc
      421 gatgtctgat tcattttttc tgcgagacca cgtgacacca gattaactac gaattgtcga
      481 ttgtctcgaa tattgacgag tgtgtctttt tcgcgacctt ccggcgtatt tactgggcag
      541 aacaacactg acggtgggtt actgcaaact ccattaaaaa acgaaaacgg tgctaggttg
      601 ctcacgccag atttgttcac tgtgctcacc caagctatcg gacgaggtac gatcgagccg
      661 ataattaatt tataagcttc ttgaaacgga agtttttcaa catcgacttc gacgtgggaa
      721 cttttagggt ttggcatatt ctttccaact atttttataa tcgaccattt cacaagacac
      781 tgcggtatcg aacgccttta gggggcgagt ggtgtcgatc attacggcat attcctgcgc
      841 aaactctttg gaaaactgat ttgcgaacgc cttaggatgc ggcccgtgat gaattccctg
      901 ggggtgaaaa gtgaacattc cgggcgttat accgctgcgg ctaaaaaaat cgccgtcgtg
      961 ataaaaaatc acttcgtcga attcgatatt gctatgaaaa aatggcactc tctgcgcttt
     1021 cggatccccg gtctccattg ggcgtggggc gaatgtgcaa agtacaaagt ttttggccat
     1081 taacgtggtg tgtacgcttg gcggcaaatg aaagcgtggg ctaataatgg gtcgaatatc
     1141 ttcaacgtta aaacggtaag caaaggcatc cccacgccaa ccaacgacgt taagtggatt
     1201 gaaagggtaa tacacgtcag tgtattcgtt ttgtcgctta attttaagtt tccactcttt
     1261 gccgtcggtc gctggtgcgc cactggcagc ggcctgcgga acatcaatgc aagcagggtc
     1321 taaaaaagcg tgacgaccga gagagccacg atcgggaagt tcaaactcgg aaaacgattc
     1381 gatcaataga taaaattggg gagccccagc gtccggaaaa aaacgataag tcgttcctcg
     1441 cgggacaacc aaataatcgc ctttccggta tttgagaggg ccaaaatctg tttctagaaa
     1501 acccgagcca gcgtgaatga accaggcttc gtcgccgtcg gcgtttcgaa catagtgagg
     1561 agtttgtttt tggggtgcgt aaatccaaag gcaaacatcg tcgtttccta aaaaacgttt
     1621 tggggcagta gtcgccgttc cgagtgctgc gttggcatcg tacgcatgag ggtgaagtgg
     1681 cccgtcgatt cgtgtccaac cagtgggcgc atttttacgg taaaggtgag tggtgcgccc
     1741 gaaaaaacct tggcgaccgt attcttcttc gaaggtacca tttggcaagt caacgtgagt
     1801 ttgcttcgat accgtgcccg atgaaaatgg aatttgtggg ggtttatggg acatgctcag
     1861 ttcctctata ggttggtcgg attttttagg gtttggcgcc gacacgattt tgaagctcgc
     1921 cgattccttc tatttttaaa gtcactgtat caccgggagt cagccaacgg tcaagttcaa
     1981 ggccgcaccc ccggcccact gtaccggaac cgtagagatc ccccgcgaaa accggttcgt
     2041 cgcggctcac gtgcgcaatc atatcagaaa aactccagtg ccgagtggcg gagttgccat
     2101 cgctccagca ttggtcgttg ataaatgctt gcatccgaag gccgttggcg tcacccaatt
     2161 cgtctttggt aacgatacaa ggaccaatac tcgttccaaa gtctttgcct tttgcgggtc
     2221 caagccgaca gagcatttct ttcgcctgaa tatcgcgtgc gctccaatcg ttcatgattg
     2281 cgtagccgaa aatgtgatcg agagcctttt ctttttttat gttcacgccg cttcgaccga
     2341 ggaccatcgt gagttcaagt tcgtaatcga gtttctgggt gaacgatggc caacgaatcg
     2401 tatcaccagg accccaaagt gtgcggtgat tacctttgta ataaactggc atttcgtacc
     2461 actcaggagg taaatcgagt ccgcgttttt tccagctcca ggaagagtgt tcttcaaatg
     2521 ctaaaaaatc ccgcatcgat ggaggacgtg gaatcggcga taaaagcgta agtcgagaaa
     2581 ccggtactcg gcccgcgacg cgctgagatt tatttttctt gagcctaaca gcactgccaa
     2641 gttgcttcca ggcgcttgga cctgcctcaa ttaagtgcag caatgtcggg actttttttg
     2701 aggtaaagtc aaatacgtcg ttgtcgatcc aaacacccca acgcgcttgg gctgcgcggt
     2761 tccaggcgta gtcgttgggc ttgtcggttc ggcgataggg tttagataga aaagtaacga
     2821 gcttcattta aggtcgtacg cggtcaatca aaacatcaac tccaccggac cctacaagcg
     2881 cggggttgtc tttgtgaacg ccctcaatat cgcccgcagc aggcattgcg tcgccggaac
     2941 gcgaaaggcg agcggcaatc gtaagttggg cgcccgcttc gaatccacct gtaccgccca
     3001 tcatcgcgtc ttccgggccg atcacatatt ccaagggaaa cgttgggttc acgattcgtt
     3061 taaccgcagt aggtgggccc gactgttggt ttcgggcaat gatgaaaaga gtgtccgttg
     3121 gttttaaaga cggcaccagc tgttgatcga taacgattct tcctgaaatt tttccgggag
     3181 tcgcagtggt cttatttttg gtacagccaa acgcgaaacc aatcgcaagc gcgacgaaag
     3241 taacttttga tacgaaatta aaattcataa acgttctcca gaatgatgcg gtgggtatag
     3301 aaacaaattg ctccatcatc caaaattatg ttgcaacact tttaagtctt catagactga
     3361 aagagtcggc caaacaatgt cgaaacgcga gcgtgaatgg gaaattccct ttttgatgat
     3421 gtggtgcgtc tcactggatt acccgaggaa acaattttcg gtgagctttc cgatattgtc
     3481 ggtaaagtcg gggcagagcc caaaagttta acaatcgagc aactgcgcgc ggcaatgttg
     3541 tgctaccttg aggaacttgc cagtttcgag gagttctcta ttgatgaaac accccaaagt
     3601 cctgtaaaaa taataaaaga ggataacccc tcttgatcgg acttcgcccc ttagaaattc
     3661 catgcaattg agttcgcaac ggagagccaa atgaatcgca accaagccct tgaagtcctc
     3721 tctcagtata taccgaatcc gatcgtcgtt gagcaaacgg cacggggcga acggcaatac
     3781 gacatttata gtcggctgct gcttgaccgg atagtttttc tgggcactga ggtgaatgat
     3841 acggtcgcca acctcatcgt ggctcaattg tttttcttag aaagtcagga ccccgaaaaa
     3901 gacgttcatc tgtatattaa ttccccgggt gggtccgtgt cggcgggcct tgctatttac
     3961 gacgtaatgc aatttataaa atgcgacgtt tcgacctatt gtttgggaat ggcagcgagc
     4021 atgggatctt tgctcctttg cgcgggcgca aaaggtaagc gctactcgct acctcatagc
     4081 cgtatcatga ttcaccagcc actgggtggt gcccgagggc aggcaaccga tatcgagatt
     4141 caagctaagg aaattcttta tttgcgggct cgtctcaatg aaatttattc gcggcata
//
LOCUS       DAOMXO010000131         4156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11589_length_4156_cov_5.176055, whole genome shotgun sequence.
ACCESSION   DAOMXO010000131 DAOMXO010000000
VERSION     DAOMXO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11589_length_4156_cov_5.176055"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLH57_03810"
     CDS             complement(<1..211)
                     /locus_tag="PLH57_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRK01765.1"
                     /translation="MKVWSCEDSVRLYNIENWGLHYFGTNAKGNLTVRPRQTAAAEID
                     LKGVIDDIISRGIKLPVLVRFQDILR"
     gene            complement(229..864)
                     /locus_tag="PLH57_03815"
     CDS             complement(229..864)
                     /locus_tag="PLH57_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01766.1"
                     /translation="MYQKYLDPVIEEFTTGKWYEEVFKAKQEYFERAGVVYEDDSEFE
                     NRMCIFMDWYLFDRELPTIDLPPIKFYFRQNKDKFSSEELNIYKDFCETVHSIFYLKK
                     ISGKTIHLKDLFSKKSYEVDDGEINWGFVKGDIFEARLVLFKGVYEFSRGFCFHPVEM
                     EKFILGEIKKVRYQEGSRQTKLILQLAAMKLKHTRYQHINIRHIYTFDPKF"
     gene            complement(884..1969)
                     /gene="carA"
                     /locus_tag="PLH57_03820"
     CDS             complement(884..1969)
                     /gene="carA"
                     /locus_tag="PLH57_03820"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRK01767.1"
                     /translation="MNNKIPGVLLLEDGTEFSGSLIGARTRGFGEVVFNTGMSGYQEI
                     LTDPSYWQQLVVMTYPHQGNYGINDSDSESEGGPKVGGFIVHEIESLPSNFESTEDLQ
                     TYLERHNVTGLSGVDTRSLTVHIRSRGAMRGLVLSDKERASITDFAAEFAKLPKFEGR
                     DLILEVTTKKPYWYSEKGKKTVVALDFGVKTNLLREIAMRDCKIVVLPANSTKKEILS
                     YHPDGVFLSNGPGDPAVATYAIETVKQLLGETPIFGVCMGHQILGHALGGRTYKMKFG
                     HRGANQPVADLGSGRVEISSHNHGFAVEAGSLPPGVTVTHRNLNDETCEGIECLEKRA
                     FSVQYHPESAPGPHDSSYLFDRFIGMI"
     gene            complement(1966..3246)
                     /locus_tag="PLH57_03825"
     CDS             complement(1966..3246)
                     /locus_tag="PLH57_03825"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRK01768.1"
                     /translation="MTDRNFLLKNVNIINPAGTFSGTGELLVENGRIRAMDRPGKISK
                     PSGFEEIDGKGAACVPGLVDIHVHFREPGFEYKETIATGSVAAAAGGFTSVACMANTN
                     PVNDNPFVTQFIRAQAREHAVVNVYPIGALSVGLKGERLAEIGKMRDAGIVAVSDDGM
                     PVMNSYLMRKALDYCKTFDLPIISHAEDANLVGQGVMHEGFHSCCLGLRGIPAESEEI
                     MVARDIALARLTGGRVHFAHLSTRYALEHVRRAKSEGLKITCEVTPHHFTFRDEDVSS
                     YDSHFKMAPPLRTEADILALHQALADGTIDCIATDHAPHAPRDKNVLFEFAANGVVGL
                     ETAWSAGLNLVENGTVSLERLVELLAVMPAKVIGVDGGILKENGKADFFLTDLSAVYT
                     LTVDELRSKSKNSPFIGRTLKGKVVSTFVAGKLV"
     gene            complement(3239..>4156)
                     /locus_tag="PLH57_03830"
     CDS             complement(3239..>4156)
                     /locus_tag="PLH57_03830"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRK01769.1"
                     /translation="NRSLVQTLGLSESDVRVVLTRAQELKKMWLGWKGSEFPHASLKG
                     RTVVNLFFESSTRTRSSFELAARRLGGDVLNFTAETSSVTKGETLFDTARNLEAMEPD
                     LLVVRHASAGAPLQLSKMIKVPLINAGDGFHEHPTQAFLDVFTIEEHRGTVKGRHVLI
                     VGDIAHSRVARSNIYLLKTLGARVSVCGPPSLMPPSIEKLGVSVFHDLRKALESADVV
                     MMLRIQLERQNQMQFPSLGEYSKFWGLNAETIGSLKEKAIIMHPGPLNEGVEISQDVA
                     TGSYSVIMDQVRNGVIVRMALMDLMVGHD"
BASE COUNT         1073 a         1111 c          901 g         1071 t
ORIGIN      
        1 gacgcaaaat atcttgaaag cgcaccagca cggggagctt aatgccacga gaaataatgt
       61 cgtcgatcac acccttaagg tcgatttcag cggccgcggt ttgtcgcggg cgaactgtca
      121 gattaccttt ggcgttggtg ccaaaatagt gcaaacccca gttctctata ttgtaaagcc
      181 taacggaatc ttcgcagctc caaaccttca tttcaccgcc tataccatct aaaacttcgg
      241 atcaaaagtg tagatatgac gaatattgat gtgctgatat cgcgtgtgct tcaacttcat
      301 cgcagcgagc tgcaaaatca gtttcgtttg acgcgaaccc tcctggtagc gcactttttt
      361 gatctctcct aaaatgaact tttccatttc taccgggtga aagcaaaacc cgcgggaaaa
      421 ctcataaaca cccttaaaca gtacaagtcg tgcctcaaaa atgtcaccct taacgaagcc
      481 ccagttgatc tcgccatcgt ctacttcata gcttttcttg gaaaacaggt ctttaaggtg
      541 aatcgtcttt ccgctgatct tctttagata gaagatgctg tgcacggttt cgcagaaatc
      601 cttgtatata ttgagctcct cagagctgaa cttgtcctta ttttggcgaa aatagaactt
      661 aattgggggt aaatcgatag taggtaactc ccgatcaaag agataccaat ccatgaaaat
      721 acacatccga ttttcaaatt cggagtcgtc ttcgtaaaca acgccagcgc gttcgaagta
      781 ttcttgcttc gctttaaata cctcttcgta ccactttcct gtggtgaact cctcgatcac
      841 ggggtccaaa tatttctgat acatgccgtc ctttgccttc gaatcagatc attccaataa
      901 aacgatcgaa caaatagctc gaatcgtgag ggccgggtgc tgattcaggg tgatattgta
      961 cagaaaaagc gcgcttctcc aaacactcta taccttcaca ggtttcgtca ttgaggttgc
     1021 ggtgtgtcac cgtcactcct ggtggaaggg agccggcctc cactgcaaat ccgtgattat
     1081 gggaagaaat ctcaacgcga ccgcttccga gatctgccac tggctgattg gcaccacgat
     1141 gcccgaactt catcttgtag gttcgaccac ctagggcgtg ccctaaaatt tgatggccca
     1201 tacagactcc aaaaataggc gtttctccaa gcaactgctt cacggtttcg atagcgtaag
     1261 tcgccacggc gggatcgccg ggaccgttgg acaaaaacac cccatcaggg tgatagctca
     1321 atatttcttt tttggtcgag tttgccggca gcacgacgat tttacagtct cgcatggcaa
     1381 tctcacgcaa aagattcgtc tttacgccaa aatctagggc aacaacggtt ttttttcctt
     1441 tttcggagta ccagtaaggc tttttggtag tgacttcgag tatcaagtct cgcccttcaa
     1501 attttggcaa cttcgcaaac tcggccgcga aatcggtgat gctggctcgc tctttgtctg
     1561 aaagtactag cccgcgcatt gctccccgag aacgaatatg aacggtgagc gagcgggtat
     1621 ctacaccgct gagcccagtg acgttatgac gctccaaata agtttgtaga tcttcggtgg
     1681 attcaaaatt actcggaagt gattcgattt cgtgaacaat aaagccgccc actttggggc
     1741 caccttcgga ctcgctgtct gagtcgttga ttccgtaatt tccttgatga ggatacgtca
     1801 tcacgactaa ctgctgccaa taactggggt ccgttaaaat ttcctgataa ccactcatgc
     1861 cggtattaaa gactacttcc ccaaaaccac gggtacgggc accgatcaga gatcccgaaa
     1921 actcggttcc atcttccaga agcaaaacac ctggaatttt gttgttcata caagtttacc
     1981 cgctacgaat gtggacacta ccttaccctt gagggttcgc ccaataaagg ggctattctt
     2041 actcttcgat cttaattcgt cgaccgttaa ggtgtagacc gccgatagat ctgtcagaaa
     2101 aaaatcggcc tttccatttt cttttagaat gccgccatct acccctatca ccttagccgg
     2161 cattacagcc aacaactcga ctaatcgttc aagcgatact gttccatttt ccacaagatt
     2221 caaaccggcg ctccaggccg tctccaaacc aaccacaccg ttggcagcga actcaaataa
     2281 tacgtttttg tcccttggag catgaggggc atgatcggtc gctatacagt cgatcgtacc
     2341 atcggccagg gcttggtgta gcgccagtat atcggcttcc gttcgcagcg gaggtgccat
     2401 tttaaaatga ctatcgtaac ttgagacgtc ttcatcccga aaagtaaaat ggtgaggcgt
     2461 tacctcacag gtgatcttaa gaccctcgct ctttgcccgc ctcacgtgtt cgagcgcgta
     2521 acgagtactc agatgtgcaa agtgaacgcg ccctcccgtt aaacgagcca acgcgatgtc
     2581 tctggcgacc attatttctt cgctctccgc tggaattcca cgtaaaccca agcaacacga
     2641 atgaaaacct tcatgcatca ctccctgacc tacgagattc gcatcttcgg cgtgggaaat
     2701 aattgggaga tcaaaggttt tacaataatc gagggctttg cgcatgaggt agctgttcat
     2761 gaccggcatt ccgtcgtcgc tcaccgcgac gatgcccgca tcgcgcattt ttccgatttc
     2821 cgcgaggcgc tcacccttta agccaacaga cagcgcaccg atcggataca cattaaccac
     2881 cgcgtgttcc cgagcctgag cgcgaataaa ctgagtgaca aacgggttgt cgttaacggg
     2941 gttcgtgttg gccatacagg ccaccgaagt aaaaccaccc gcggccgccg ccacacttcc
     3001 tgtggcaatc gtttctttgt attcaaaacc gggttctcta aaatgaacgt gaatatcgac
     3061 caatcccgga acgcaggcag ctccttttcc gtcgatttct tcaaacccgc tcggtttaga
     3121 gatctttcct gggcgatcca ttgctcgaat acgtccgttt tcgaccagaa gttccccagt
     3181 accggaaaaa gttcccgcag gatttataat attaacgttc tttaaaagaa aattcctgtc
     3241 agtcatgacc caccattaaa tccatgagcg ccattcggac tattactccg ttgcgaactt
     3301 gatccatgat caccgaatag gatccggtcg cgacatcttg tgaaatttcg acgccctcgt
     3361 tgagtggccc cggatgcatg ataatcgcct tttcttttag ggatccaatc gtttcggcat
     3421 ttagtcccca aaacttcgaa tattctccca aacttggaaa ctgcatctga ttttgccgtt
     3481 ccaattgaat tcgcaacatc attacgacgt ccgcactttc gagagctttt cgtagatcat
     3541 gaaaaacaga caccccgagt ttttcgatcg acggtggcat taaactagga ggaccacaca
     3601 ccgaaacccg agcgccaaga gttttgagca gataaatatt ggaacgcgca acccgcgaat
     3661 gagcaatgtc gcctacgata aggacgtgcc gccccttgac cgttccgcga tgctcttcga
     3721 tcgtaaaaac atcgagaaaa gcttgagtgg ggtgttcatg aaaaccatca cctgcattta
     3781 taagcggtac ttttatcatt tttgacaact gcaacggcgc tccggccgag gcgtgccgaa
     3841 cgaccaatag gtctggctcc attgcctcta aatttcgggc agtgtcaaaa agagtctccc
     3901 cctttgtgac cgaagacgtt tcagctgtaa aatttaaaac atcaccaccg agtcgccgag
     3961 ccgccaactc aaagcttgat cgcgtgcggg tactggactc aaaaaaaaga ttcactaccg
     4021 tgcggccctt taagctggca tgagggaact cggacccctt ccaacccaac cacatttttt
     4081 ttaattcctg agcacgggtc aacaccacac gaacatcgga ttcgctcaat cccagtgttt
     4141 gcaccaaact gcgatt
//
LOCUS       DAOMXO010000132         4126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11709_length_4126_cov_5.328666, whole genome shotgun sequence.
ACCESSION   DAOMXO010000132 DAOMXO010000000
VERSION     DAOMXO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11709_length_4126_cov_5.328666"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLH57_03835"
     CDS             <1..510
                     /locus_tag="PLH57_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRK01770.1"
                     /translation="GSLNPTYDPGDVMVIQDHVNLTGHNPLIGPNPTDSKTKKPLGPR
                     FPDLRNLYDREISAQLLRSLDQQKMRAHLGTYLGVLGPSFETPAEVRLFSKWGLDAVG
                     MSTVWEAIALAHSGATVAGVALISNVGCGLRDDAPLDHHEILRTSKAAAQKIISSVFQ
                     VVANLPEDR"
     gene            510..1871
                     /locus_tag="PLH57_03840"
     CDS             510..1871
                     /locus_tag="PLH57_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRK01771.1"
                     /translation="MNEFDLGIRCRRLIAMTDSSNTPGEMVSENMFLGIRESNIAAIE
                     PYTEELWGRCKKRIDANHHVVLPGLINTHTHLAMTLFRGFEDDVPFHQWLFERILPME
                     NELVDPDFVRVGTMLGAAESLRNGVTTINDMYFYANEIAQVCEQAGIRACVAQVWSNF
                     PLPENKTLGTGNIVQFTERFQSLLSNWSHHSRITPTLGPHAPYTCDDDLFRAVVALSD
                     KTGAPIHVHLSETKFEVEESLKKFSKTPVKRLADLNVLKRGTLCAHAIHCDEIDTEIL
                     RESGASVAYNPDSNLKLGSGIAPITNYLKHGIPVGLGTDGAASNNSLSIFGAMNIGAK
                     LQKLSQHDTTAMTAPQALHAATLGGARALGLSDRIGSLEVGKRADLICIDFDQPHLQP
                     INDPVSHLVYSATGGDVTHVICDGHIKVKDRVLQGINTAELYGKAEQWRKTLYAKLSN
                     MKS"
     gene            1826..2485
                     /locus_tag="PLH57_03845"
     CDS             1826..2485
                     /locus_tag="PLH57_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRK01772.1"
                     /translation="MAQNAIRETLEHEIVDVCQRLHTKGMLAAADGNVSAVFENEILI
                     TPTGRSKARVAPSEMAALTRTGEILRGSPSGERLMHLAVYRQCAKAKAVVHAHPPAAI
                     AWSIAYPELLELPAHAMSELILATGRIPIAPYARPGTAEMGSVLESLLPKHRVIILGR
                     HGAISWGEDLEEAYNGMERLEHAALILKYAKELGQITDLPKTEIDALFEIRKKLGDRT
                     L"
     gene            2482..3498
                     /gene="mtnA"
                     /locus_tag="PLH57_03850"
     CDS             2482..3498
                     /gene="mtnA"
                     /locus_tag="PLH57_03850"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010558255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRK01773.1"
                     /translation="MKNLVSLGLRHENQKLFILDQTLLPHKEQWIECHTPLEMIALIQ
                     RLAVRGAPLIGVAASVALARFAENGATSEQVLEAAHALRAARPTAVNLMHAIDEVALN
                     SRDLSVQGIVQRTEALFDEDVSLCHEMAELGSGLISDGDHILTHCNAGALATVGAGTA
                     LGVITRAHEKGKKIHVYVDETRPLLQGARLTVWELQRAGVPCTLICDNMAGFLMQRGK
                     IHKIFVGADRIARNGDAANKIGTYSVAVLAKAHQVPFYVVAPHTTIDLKCENGSEIPI
                     EQRDSQEVRTDKAPLDCNVTNPAFDVTPAHYITGIITTRSGDSEKYSAMVWAPGGKPL
                     ANSF"
     gene            complement(3452..>4126)
                     /locus_tag="PLH57_03855"
     CDS             complement(3452..>4126)
                     /locus_tag="PLH57_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01774.1"
                     /translation="ILGLCVSGPAAAASENGGDDSITESLSCDETVSGSHLGWVARYE
                     IEDRFVGNWTLVSEQHDPWLGHNSTQQMPFPEDLFIRELDRKNGIELVTAQSKRLLGG
                     KVFRSINAGPVLESISGYSEPYVQYGDPIFNRNSAVQMERQIYRGEALVSMRFQAYGP
                     KFPWSLMVPARYWDRFEFAFRTTLKLIPSKDNDEMVIEFTREYGKIYGNLKNYSRAVY
                     RRAPKP"
BASE COUNT         1066 a         1128 c          981 g          951 t
ORIGIN      
        1 ggtagtttga accccaccta cgaccccggt gatgtgatgg tcattcaaga tcacgtgaac
       61 ctcaccggcc acaatcccct cattgggccc aatccaacgg attcaaaaac taaaaagccc
      121 ctgggcccca ggtttccaga tctgagaaac ctgtacgacc gcgaaattag cgctcaactc
      181 ctgcgctctc ttgaccaaca aaaaatgcgt gcgcatctcg gaacctattt aggagtgctg
      241 ggtccaagtt tcgaaacacc cgctgaagtt cgtttgtttt ccaaatgggg cctcgacgcc
      301 gtgggaatgt cgacggtttg ggaagccatc gccctcgccc actcgggcgc aaccgtcgct
      361 ggagtagcac tgattagcaa tgttgggtgc gggcttcgcg acgatgcgcc gttggaccac
      421 cacgaaatat tgcgtacgag caaagccgct gcgcaaaaaa ttatttcctc agtgtttcag
      481 gtcgtcgcta atttaccaga ggaccgatag tgaacgagtt tgatttagga atacgttgcc
      541 gtcgactcat tgccatgacg gactcctcga atactccagg ggaaatggtt tccgaaaaca
      601 tgttcctcgg aattcgcgaa tcgaacattg ctgccattga accgtacact gaagaactct
      661 ggggtcgctg taaaaaaagg atcgacgcga atcatcacgt ggtcttgcct ggtttaataa
      721 acacccacac tcaccttgcg atgacgcttt ttcgtggttt tgaagacgat gttccttttc
      781 accagtggct cttcgaacgc attcttccga tggaaaatga actggtcgat cctgacttcg
      841 tacgagtcgg taccatgctc ggtgcagccg aaagtttgcg gaacggcgta accaccatca
      901 acgacatgta tttttacgcc aacgaaatcg cccaagtttg tgagcaagcc ggcattcggg
      961 cctgcgtcgc gcaagtgtgg tcgaactttc ctctccccga aaacaaaact ttgggcactg
     1021 gaaacatcgt ccaattcact gaacgctttc aaagtctttt gtctaactgg tcccatcatt
     1081 cgcgcatcac accgacgctt ggaccgcatg ctccgtatac ctgtgacgac gacttattta
     1141 gagcagtcgt agccctaagt gacaaaacgg gggcccccat tcacgtgcac ctgtcagaaa
     1201 ctaagtttga agtagaagag tcgttaaaaa aattctcaaa gacgcccgta aaacgcctcg
     1261 cagatctgaa cgttctcaaa cgcggcacac tttgcgctca cgcgattcac tgcgatgaaa
     1321 tcgataccga aatacttcgc gaatccggcg cttcggtcgc ctacaatccg gattctaacc
     1381 ttaaacttgg atctggaatt gcgccaataa ccaattattt gaaacatggc attccagttg
     1441 ggctcggcac cgacggtgca gccagcaata acagtcttag catttttggt gccatgaaca
     1501 ttggggcaaa acttcaaaaa cttagtcaac acgacactac cgcaatgacc gcgcctcaag
     1561 cacttcatgc tgcgacactt ggcggtgcaa gagcacttgg actctcagat cgcatcggca
     1621 gtttggaagt gggaaaacga gcggacttga tttgcattga tttcgatcag cctcatcttc
     1681 agccaatcaa cgaccccgtg agccacctcg tctattcggc cactggtggc gacgtaaccc
     1741 atgtaatttg cgacggtcat attaaagtaa aggaccgagt actccaggga attaacaccg
     1801 ctgaacttta tggaaaggca gaacaatggc gcaaaacgct atacgcgaaa ctctcgaaca
     1861 tgaaatcgta gatgtctgcc aacgtttgca caccaagggc atgcttgctg cagccgacgg
     1921 aaacgtaagc gcggtttttg aaaacgagat tctcatcacc cccactggtc gtagcaaagc
     1981 gcgcgtcgcg ccgagtgaaa tggccgctct tactcgaacc ggggaaatac ttcggggctc
     2041 tccgtcgggg gaacggctca tgcatctagc agtttatcgc cagtgcgcca aagccaaagc
     2101 agtagtgcac gctcatcccc ctgccgccat tgcgtggagc attgcatatc ccgaattact
     2161 tgaactaccg gcccacgcga tgtcggagct aattttagca acgggtcgca ttccgattgc
     2221 accctatgcc cgcccaggca cggcagaaat gggctcggtc cttgagtcgc tacttcccaa
     2281 acaccgcgtg atcattttag gtcgccacgg cgcaatcagt tggggcgaag atctcgagga
     2341 ggcctacaat ggaatggaac gactcgaaca tgcggcactt atattaaaat acgccaaaga
     2401 gttgggacaa atcaccgatc taccgaaaac cgaaatcgac gcgctctttg aaatcaggaa
     2461 aaaactagga gatcgaaccc tgtgaaaaat cttgtgtcac ttggcttgcg ccacgaaaat
     2521 caaaaactct ttattctcga tcagaccctg ttgccacaca aagagcagtg gatcgagtgc
     2581 cacaccccct tggaaatgat cgcccttatt caacgccttg ctgtacgggg cgccccactt
     2641 attggtgttg ccgcgtcggt tgctctcgcc cgttttgcgg aaaacggagc tacttcggaa
     2701 caagtacttg aagccgccca cgctctgcgg gcagcacgac ctactgcggt caatctcatg
     2761 cacgccatcg acgaagtcgc tctgaattcc cgcgatcttt cggttcaagg aatcgttcaa
     2821 cgaaccgagg cactgttcga cgaagacgtg agtctttgtc acgaaatggc tgaacttggg
     2881 agtggcctca tttcagacgg cgatcatatt ctcacccatt gcaatgccgg ggctctcgcc
     2941 acagtaggcg caggaactgc tctgggcgta atcacccgcg ctcacgaaaa gggcaaaaaa
     3001 atacatgttt atgttgatga aacacgccca ctattacagg gcgctcgttt gaccgtttgg
     3061 gaactgcagc gagccggcgt tccgtgcact ttgatctgcg acaacatggc gggtttttta
     3121 atgcagcggg gaaaaatcca taaaatattt gtcggagcgg accgtattgc acgcaatggt
     3181 gacgccgcca acaagatcgg aacctattca gtggcggtgc tcgcgaaagc ccaccaagtt
     3241 ccattttacg tggtagcgcc ccacacgacg attgacctaa aatgtgagaa tggatctgaa
     3301 atacctatcg aacaacgaga ctcacaagaa gttcgcacgg acaaagcgcc actggactgc
     3361 aacgtcacta accctgcgtt tgacgtaact ccggcccact acattactgg aataataact
     3421 acccgtagcg gcgattctga aaaatattcg gctatggttt gggcgcccgg cggtaaaccg
     3481 ctcgcgaata gtttttgaga tttccgtaga ttttcccgta ctctcgcgtg aactcaatca
     3541 ccatttcatc gttgtctttg ctgggaatca gcttcagtgt ggtgcgaaaa gcaaattcga
     3601 aacggtccca atatcttgcc ggcaccatca aactccaagg aaacttggga ccataggcct
     3661 gaaatcgcat gctcactaga gcctcgcccc ggtaaatttg acgctccatt tgcacggcgc
     3721 tgtttcgatt gaagatcgga tcaccgtatt gaacataggg ttccgaataa ccgctaatgc
     3781 tctccaatac tgggcccgca ttaatcgatc gaaaaacctt accgccgagc aaccgtttag
     3841 actgggcagt tactaattcg atgccgtttt tacgatccaa ctctcgaatg aatagatctt
     3901 cgggaaaagg catttgctga gtgctgttat gtccgagcca cggatcatgc tgctcactta
     3961 cgagcgtcca attccctacg aatcgatctt caatttcgta tctagcgacc caacctaaat
     4021 ggctcccact cacagtttcg tcgcaactta aactttcggt tatcgaatca tcgccgccgt
     4081 tttcggaagc ggccgctgcg ggcccagaaa cacatagacc caaaat
//
LOCUS       DAOMXO010000133         4113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11763_length_4113_cov_5.239034, whole genome shotgun sequence.
ACCESSION   DAOMXO010000133 DAOMXO010000000
VERSION     DAOMXO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11763_length_4113_cov_5.239034"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..957
                     /locus_tag="PLH57_03860"
     CDS             307..957
                     /locus_tag="PLH57_03860"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRK01775.1"
                     /translation="MSVTAVAAGLGLLQIRWYGWYLFLFSSFTTIYQTAIALTYYSSA
                     ENKFPVFAVYAVFQLLLVYLIAREVRVPYYFPRIRWWESDPRYKLSIPTRIEKNAEIY
                     NGDIMDLSMSGCFIKTREYFLPDDSVTLEFSLFEKTISCAGKVVWRTESTVTHPKGIG
                     VKFSVLDRDTSTALKQATHKLRKLIQEHNNKTREKNWEEYLAREKQYQSRTKVPKK"
     gene            954..1409
                     /locus_tag="PLH57_03865"
     CDS             954..1409
                     /locus_tag="PLH57_03865"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRK01776.1"
                     /translation="MNLFLNEALKEAIKAEKKNEVPVGAVVVKNGRVIARAHNLRETR
                     QDPLTHAELIAISKAAKKVGSWRLVDCEVYVTLEPCVMCAGALSQARVKAVFYGALDP
                     KGGAQSLKVRIHDNPKMNHRYDLVHLPSAECGQILSEFFKKKRKERKSK"
     gene            1406..2008
                     /locus_tag="PLH57_03870"
     CDS             1406..2008
                     /locus_tag="PLH57_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01777.1"
                     /translation="MIRAQTEPPVRLAPPGKGLPLIERLLAKYILLPKMTRKLDANGA
                     KIFFEKETQKILTLAESVSPEHFQKPILIDRLGGLEDSSRFWSVEMTLEHLVIVANAM
                     DILIRDLLAGRHPSLQVDIAKVKPHGGANDKAAVIATFRTQMMDLINKHPSIVGDSSV
                     MHPHPWFGAIDAHTWMVVRALHQRIHRKQIENIIRGLNLL"
     gene            complement(1994..2929)
                     /locus_tag="PLH57_03875"
     CDS             complement(1994..2929)
                     /locus_tag="PLH57_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRK01778.1"
                     /translation="MGIMNWPLPKKVRLFEVGPRDGLQNEKATLSLEAKVDYIEALLE
                     SGLKDVEVGAFVREDKIPQMANTAEILQRLRDRGYEKRKGLTLWTLVPNEKGLDRARE
                     AGARAIAVFTGATETFTQKNIGMSVKDSLKVFEVVAKQASKDRIRVRGYVSVCWGCPY
                     EGKVKPSNVVKLAKQLLDLGVEQVSLGDTIGVATPLEVVRLLLSLKREIARDEIAVHF
                     HDTRGTALPNCLAAVGFGVRTIDASSGGLGGCNYAPGASGNVATEDLVYMLEGMGIKT
                     GVSLDRLCAASEKAIRALDRLPTSKYLQAWLSKKV"
     gene            complement(2929..>4113)
                     /locus_tag="PLH57_03880"
     CDS             complement(2929..>4113)
                     /locus_tag="PLH57_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcrotonoyl-CoA carboxylase"
                     /protein_id="HRK01779.1"
                     /translation="EALGSFGSGEIFLERYLAASKHIEVQVLGDRIGKVIALGERDCS
                     SQRRHQKVIEEAPAPNLLQKTREALCKAAVDLAASVKYESAGTCEFLVDESGNIYFLE
                     MNTRLQVEHPVTELVWSVDLVAAQIQIAQGKTLREIIPHVDNLSARGHAIELRVYAED
                     PAKGFMPQPGELGEVRWPSGPFVRVDQGFESGSTVSIFYDAMLGKVIAWGMIREQART
                     RVLRAICETEIEKVRWNGDYLRDILEHEDFIDGNVTTQFLGEKFANWKPSPRTKSDQK
                     KVTTARSNPNADAAQSPWIVYGDATVAATKRYSKAGVGVAATVGVVGGPLCSDYPGKV
                     LKVNVKAGDLVAEGQALIVCESMKMEFTYAAPAKAKVANVNVKTGMVIAAGAVLIEWE
                     TT"
BASE COUNT         1061 a         1044 c          881 g         1127 t
ORIGIN      
        1 gatccggtcg attgtacacg catgatggcc tcccttgttt gtatatatga tcattatagt
       61 acggtattaa accggtttcc agcaggatcg gggcatctaa cttattgata tatttgataa
      121 agtcatctac gcttaacaat aataaggaat cacccattgg ccaataaaca cgcttcaaaa
      181 aaacttaagc ttttcgcatg gttatggatt tcctttcctt tcctgtactt agctatctgt
      241 ctcagctttt tcgagttgga cttcgggggt gttctcaaga tcgtccttag tccctggtac
      301 tggttcgtgt ccgtaactgc ggttgctgcg ggtctgggct tgcttcaaat tcgatggtac
      361 ggttggtacc tttttctgtt ttcgagtttt acgaccattt accaaaccgc catcgcgcta
      421 acttattatt cgtcggccga aaacaagttt ccggtattcg cggtttacgc cgtttttcaa
      481 ctacttttag tttatttgat cgctcgcgaa gttcgtgtgc cctattattt tccgcgaata
      541 cgctggtggg aaagcgaccc acgttacaag ctcagtatcc ctactcgcat cgaaaaaaat
      601 gccgaaatct acaacggcga tatcatggac ttgtcgatgt ctggttgttt cataaaaact
      661 cgcgagtact ttcttcctga cgattccgtt acccttgagt tttcgttgtt cgagaaaact
      721 atcagctgtg ctggaaaagt agtttggcgg acggagagca cagttaccca tccgaaagga
      781 atcggcgtta aattttctgt tttagatcga gacacgtcga ccgctttaaa acaggcaacc
      841 cacaaattgc gcaaactaat tcaagagcac aacaacaaga cgcgggaaaa aaattgggaa
      901 gaatacttag ctcgcgaaaa acaatatcaa agccgaacga aggttcctaa gaagtgaatc
      961 tttttttgaa tgaggccctt aaagaagcaa ttaaggccga aaaaaagaac gaagtcccgg
     1021 tgggggcggt tgtcgtaaaa aatggacgtg ttatcgctag agctcacaat cttcgagaga
     1081 ctcggcagga tccgttaacc catgccgaac ttatcgcaat ctctaaagcc gcaaaaaaag
     1141 ttggatcgtg gcggttggta gattgcgaag tttacgtaac gctagagcct tgtgtcatgt
     1201 gcgccggtgc attatctcaa gctcgcgtaa aagcagtatt ttacggcgct cttgatccaa
     1261 agggcggtgc tcagtcccta aaggttagaa ttcatgacaa tcccaaaatg aatcatcgct
     1321 atgatttagt tcatctccct tccgccgagt gcgggcaaat tttaagtgaa ttttttaaga
     1381 aaaaaagaaa ggaacgtaag tcaaaatgat tcgcgcccaa acagaacctc cggttcgctt
     1441 ggccccccct ggaaagggtt taccccttat tgagcgtctt ttagccaaat atattctatt
     1501 gccgaaaatg acgcgaaagc tcgacgctaa cggcgcgaaa atctttttcg aaaaagaaac
     1561 ccagaaaatt ttgacgctag cagaatcggt ttcaccagaa cattttcaaa aaccaatatt
     1621 gattgatcgc ctcggtggtt tagaagatag ctctcgtttt tggtcggtcg aaatgacgct
     1681 tgaacatctc gtaatcgtag caaacgcgat ggatatttta attcgtgatt tacttgccgg
     1741 gcgccacccc tctttgcaag tcgacatcgc gaaggttaaa cctcacgggg gggcaaacga
     1801 caaagccgcc gtcatcgcta cgtttagaac tcaaatgatg gaccttatta ataaacaccc
     1861 aagtatcgtg ggcgatagtt cagtcatgca tcctcaccct tggttcggcg cgatcgatgc
     1921 ccacacttgg atggtcgtac gggcactgca tcaacgtata catcgcaaac aaattgaaaa
     1981 tattattcgt ggattaaacc ttctttgaaa gccacgcttg aagatacttc gaagtaggca
     2041 aacgatcgag cgcacgaatt gccttttcac tagccgcaca taaccgatct aacgagacgc
     2101 cggtttttat tcccattccc tcgagcatat agacgaggtc ttcggtggcg acatttccgg
     2161 acgcgcccgg tgcgtagttg cagcctccca atccaccact ggacgcatct attgtgcgca
     2221 caccaaaacc aacagctgcg agacaattag gcaatgcagt gccacgagta tcatgaaaat
     2281 gtactgcaat ttcatcacgc gcgatttcgc gttttagtga caagagcagt cgaaccactt
     2341 ccaaaggcgt cgctactccg attgtgtcgc ctagacttac ctgctctaca ccaagatcca
     2401 aaagttgttt tgcaagtttg acgacgttac taggctttac ctttccttcg taagggcaac
     2461 cccagcacac tgaaacgtaa cctcgaactc gaatgcgatc cttggaggcc tgtttagcca
     2521 caacttcgaa taccttgagt gaatctttga cactcatgcc aatatttttt tgggtaaacg
     2581 tttctgtggc tccggtaaat accgctatgg cacgtgcacc tgcctctcgt gcccggtcta
     2641 gacctttttc atttggtact agagtccaaa gtgttaggcc cttgcgtttc tcataacctc
     2701 gatcacgaag gcgctgcaat atttcagcgg tgttggccat ctgcggaatt ttgtcctcgc
     2761 gcacgaatgc tccaacctcg acatccttta aaccgctctc caacaatgcc tcgatataat
     2821 cgactttagc ttcaagcgag agggtagctt tttcattctg caagccatcg cgtggcccca
     2881 cctcgaagag tctaactttt tttggcaacg gccaattcat aatacccatt aggtggtctc
     2941 ccactcgatc aaaacggctc cggctgcaat caccattccc gtttttacat tgacgttggc
     3001 aaccttggct tttgcgggtg ctgcgtaggt aaattccatt ttcattgatt cacaaacaat
     3061 tagagcctgg ccttccgcta ccaaatcgcc cgcttttaca tttaccttca aaactttgcc
     3121 gggatagtcg gaacacaatg gtccgccaac tactccgacg gtggccgcca cgcccactcc
     3181 ggctttggaa taccttttcg tcgcggccac agtcgcatca ccataaacta tccaaggcga
     3241 ttgcgcggca tccgcatttg gattcgaccg cgcagtggtc actttttttt ggtcggactt
     3301 agttcgcggc gacggcttcc agtttgcgaa tttttcgccc aaaaactgcg tagtaacgtt
     3361 tccgtcgata aaatcttcat gttcaagaat gtcccgcaga tagtcgccgt tccatcggac
     3421 cttttctatt tcggtttcac aaatcgcccg caacactcgc gtccgggcct gttcgcgtat
     3481 cataccccag gcaattactt ttccgagcat agcgtcgtag aaaatactta cagtagaacc
     3541 agactcgaat ccttgatcaa ctcgaacaaa aggcccgctt ggccatctta cttcgccaag
     3601 ttctccaggt tgtggcataa acccttttgc cgggtcttct gcgtaaacac gtaattctat
     3661 cgcgtgacct cgagcactca agttatccac atgtggaatg atctccctta gtgttttacc
     3721 ttgagcaatt tgaatttgag cagctactag atctacactc caaactaatt ctgtgaccgg
     3781 atgttctacc tgcaaccgag tgttcatttc gagaaaataa atatttccac tttcatcgac
     3841 cagaaattcg caggtccccg cgctttcgta ctttacgcta gctgctaaat ccactgcagc
     3901 cttgcagagc gcctcgcgag ttttctgtaa aagattgggt gcgggcgcct cttcaataac
     3961 tttttggtgc cgcctttggg acgaacaatc tcgctcgcct agagcaataa cttttccgat
     4021 gcgatcaccc aagacttgaa cctcgatgtg tttagaggca gcaagataac gctctaaaaa
     4081 aatttcgcca gatccaaagg aacccaatgc ttc
//
LOCUS       DAOMXO010000134         4101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11806_length_4101_cov_5.022491, whole genome shotgun sequence.
ACCESSION   DAOMXO010000134 DAOMXO010000000
VERSION     DAOMXO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11806_length_4101_cov_5.022491"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLH57_03885"
     CDS             <1..644
                     /locus_tag="PLH57_03885"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRK01780.1"
                     /translation="ILGFAGAFLPSYTLGNEVERVSGVVERRTGRSHVERGDGSERVI
                     VDTGVRVFDKDLISTEKGRVHIRFRDGTLAEVGEHSKFEVDRVFQRKRVAFEKLMPLS
                     RVDESVYRLHDGVLRMTAIELDTFQQYTVKTEFAVIHVTEPSDFLMAKVNDGKQCEIR
                     LLSGKLNLVNIITNEVQELKAGYSAVMKSNGLIQSERRLSGEEVRFLKNRTGI"
     gene            654..1241
                     /locus_tag="PLH57_03890"
     CDS             654..1241
                     /locus_tag="PLH57_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01781.1"
                     /translation="MKKLFILLLFLATANAMATSRPRHQHEDLFGEDDLWLGLKAGPK
                     LSSYTDFNSISGTTRVQGEAQYALGGALKFIYAVPRIEFNVLWNVRSGINNERDLHFF
                     SFPLIVKVPFELEENFDVEIGAGAQADALIYSNHPSRRWLTGFLGTLGVSYDFVTYLF
                     EFEIRYVLTSQPLTPSIDGAKPRDLHVMAGLMWRF"
     gene            complement(1238..2830)
                     /locus_tag="PLH57_03895"
     CDS             complement(1238..2830)
                     /locus_tag="PLH57_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01782.1"
                     /translation="MLQFFTSIQRIALILGIFQITLTSSVQGAPIQQGEVEVLQAKAA
                     ILFHSKKESECLKVIDSILEADNTNKNALELRALVFKSQNKNDLAAETYLKLIEITPP
                     EKAAPYHFELGAIRFKQKQLDEAKKHFTASARRGFNKGTSHFFAGMADTSQKNWTSAS
                     KNFAASLLYPDAEPMRPVSRFYLATAHAQAGKSTAAIRNYVRTAFDVSESSKKTPGVS
                     QHGDLKKNAVDALKSFNQEQYFVTGTYLQQWDNNVTLMPYTVTTGQQATGKRTLKSIV
                     SVVAGYSSSPTKKFQFVPSNTVYTNYNYNYKSREMNFFSDTPSFYLFYKPYNRFSTGI
                     KAEGTYNMKRQLSTADGSYKYRPYSMTGDFGPLLRYEALPSLNLGYEISYKPKRYYRE
                     SKEGEDRRSGRGLYTRFTTEFNSEIEWLTPNVALTFEWDDTLGTSYRSRSYGMNITNP
                     ISFTDELTVTPGFDLLSTDYYRAVPTRTELLFTVKSSGQYSLGSNLNLLADISFTRNN
                     STQSATFTYTRLVASTGLSYSF"
     gene            2903..3829
                     /locus_tag="PLH57_03900"
     CDS             2903..3829
                     /locus_tag="PLH57_03900"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRK01783.1"
                     /translation="MTRSKKFYFLLSVIATVGFVAQRANAQTEIGRLAVVTGDVKVAK
                     NGASDAGKPAKANDPIFNGDVIKTGNGAMAKLLFTDQSIMDVGANSAFKVTDYDLKQG
                     ENRTGSFNLMYGKLRALVTKQVGEDGNVKVRTKDAVMGVRGTEFVVNQPPAIAGGTAP
                     PTQIVVVSGRVEVTPPSGGPPVSIGAGQMATAASPSSGDTTSASSSNVQVSSMTAEQM
                     SSVAEGAVAQDNTFVAAVTIAPASGDNSSGPSNNQQAQMMSLASNSAPPPPSMDSSGP
                     RPVDVPVLNSMSFLPPVNIIVGGLVRLTVSIQ"
     gene            3842..>4101
                     /locus_tag="PLH57_03905"
     CDS             3842..>4101
                     /locus_tag="PLH57_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HRK01784.1"
                     /translation="MNQKKVAEDLRAIPFAARRQHFLSQIVAQTENAVAIVPAHSELI
                     RNHDVHFKFRQDTNFYYLTGFEEPDAIAVFRNVGGKREYVLFV"
BASE COUNT         1066 a          848 c         1062 g         1125 t
ORIGIN      
        1 tgatacttgg tttcgctggg gcatttttgc ctagttatac attaggaaat gaagttgaac
       61 gggtttcggg cgtagttgag cgtcgcaccg ggcgttccca cgttgaacga ggtgatggta
      121 gtgagcgggt tattgtcgac acgggggtgc gagttttcga caaggacctc attagcactg
      181 aaaagggtcg tgtgcatata cggtttcgtg acggaacctt ggctgaagtt ggcgaacatt
      241 ctaagtttga agtagatcga gtgtttcaac gtaaacgggt ggcgtttgaa aaattgatgc
      301 ccctaagtcg tgttgacgaa tctgtttata gattacacga cggtgtactg cgaatgaccg
      361 caatcgaact tgatacattt caacagtaca ccgtaaaaac tgaatttgcg gttatacacg
      421 ttaccgaacc gagtgatttt ttaatggcca aggtgaatga tggaaagcag tgcgaaattc
      481 gtctgctatc tggaaagctt aacctggtca atatcatcac taacgaagtt caggaactga
      541 aggctggata ttccgccgtt atgaagtcaa acgggctcat acaatcggaa cgtagattat
      601 cgggtgaaga agtacgattt cttaaaaacc gaactggaat ttagatatct aaaatgaaaa
      661 aactttttat tcttttgctg ttcctggcta ctgcgaatgc aatggctact tcgcgcccac
      721 ggcaccagca cgaagactta ttcggtgagg acgatttatg gctcgggcta aaggcaggac
      781 ctaagctttc ctcctataca gattttaaca gtatttcggg aactacccgg gtccaaggtg
      841 aagcccagta tgcattgggt ggagcattga aatttatata tgctgttccg agaattgaat
      901 tcaatgtttt gtggaacgtg cggtctggca ttaataacga gagagattta cacttttttt
      961 ctttcccctt gatcgtaaaa gtcccattcg aactagaaga aaattttgat gtcgaaattg
     1021 gagcgggtgc gcaagcagac gcgttaattt attcaaacca tccttcgcgg cgttggttga
     1081 cgggcttcct cggtacccta ggggtgtctt atgatttcgt gacctatctt tttgaattcg
     1141 aaataagata cgtccttacg agtcaaccgc tgactccaag cattgacggc gcgaagccgc
     1201 gcgatctgca cgtaatggcc ggcctcatgt ggcgattcta aaacgaatat gaaagcccag
     1261 ttgaagcaac tagtcgcgtg taggtaaacg tcgcagactg ggtcgagttg tttctcgtaa
     1321 atgagatatc tgccaaaagg ttaagatttg agcccaaact gtattggccc gaagacttta
     1381 ccgtaaataa tagctctgtc cgagtaggaa cggcacggta gtagtctgta gaaagcaaat
     1441 cgaagccagg agtaacagtg agttcgtcgg tgaacgaaat cgggttagtg atattcattc
     1501 cgtaggaccg cgagcggtaa ctggtgccga gagtgtcgtc ccattcaaac gtaagtgcta
     1561 cattcggagt gagccactct atttctgaat taaattctgt cgtgaatcga gtgtagagac
     1621 cgcgaccact acggcgatct tcaccttcct ttgattcgcg gtagtagcgc ttcggcttgt
     1681 aactgatttc gtatccgaga ttcaaagaag gtagcgcttc atatcgtagt aaggggccga
     1741 agtcgccggt catactataa gggcgatatt tgtaactgcc gtcagcagtc gaaagctgac
     1801 gtttcatgtt gtacgtccct tcggctttta tccccgtaga aaaacgatta tagggtttgt
     1861 agaacagata aaagctcgga gtatcgctga aaaaattcat ctcgcgcgat ttataattat
     1921 agttgtagtt ggtgtagact gtgttgctag gaacaaattg aaactttttg gttggtgagg
     1981 atgagtagcc cgccaccaca ctaacgatcg acttgagagt acgcttgcca gtagcttgct
     2041 gaccagtggt gacagtgtaa ggcatgaggg taacgttgtt gtcccactgc tgcaaatagg
     2101 tgccagttac aaagtactgt tcttggttga aagatttcaa tgcgtcgact gcattttttt
     2161 tgagatcgcc gtgttgcgaa actcctggtg tttttttcga actttcagaa acatcgaagg
     2221 ccgttcttac atagtttcga atggcggcag tactttttcc ggcctgtgcg tgggcagtag
     2281 caagatagaa gcgagatact ggtcgcatag gttcggcgtc gggatataaa agcgaggctg
     2341 caaagttttt gctagcggaa gtccagtttt tttggctggt gtcagccatg ccagcaaaga
     2401 aatgactggt gcctttattg aaccctctac gcgcagaagc agtgaagtgt ttttttgctt
     2461 catcgagttg tttttgttta aagcgaatag ccccaagctc gaagtgatat ggggctgctt
     2521 tttccggcgg agtaatttcg attagtttca aataggtttc cgcagccagg tcgtttttgt
     2581 tttgactttt aaatactaga gcccttagtt cgagcgcgtt tttattggtg ttgtcggcct
     2641 cgagaatact gtcgataacc ttcaaacact cgctttcctt ttttgagtga aaaagaattg
     2701 cagcttttgc ctgcaatact tctacctcgc cttgttggat aggagcgcct tggactgatg
     2761 aagtcagggt aatttgaaaa ataccgagaa tcagcgcaat gcgttgtatg gaggtgaaaa
     2821 attgaagcac tatcgaactc ctttgagttg attctatatg gccgcgcgac tagtctatgt
     2881 aacgacaaga gaggtcgtca acatgacaag atcgaagaag ttttactttt tactcagtgt
     2941 gattgcaacg gtaggatttg tcgcgcaacg cgcaaatgct caaacagaaa ttggtcgcct
     3001 cgcggttgtc accggcgatg ttaaagtcgc aaaaaatggt gccagcgatg ctggtaagcc
     3061 ggcaaaagcg aatgatccga tattcaacgg agacgtaatt aaaaccggaa atggcgcgat
     3121 ggccaaactt cttttcaccg accaatcaat tatggacgtt ggcgccaata gtgcattcaa
     3181 agtcaccgac tacgatctga agcaaggcga aaatcgcaca gggtcgttta atttgatgta
     3241 cggaaaattg agagctttag ttaccaagca ggtaggtgaa gatggcaacg tgaaggtgcg
     3301 caccaaagat gcggtgatgg gagttcgggg aactgaattc gtggtgaatc aaccaccagc
     3361 cattgcgggg ggcacggctc cacctaccca aatcgtcgtt gtatcggggc gagtcgaggt
     3421 tactccaccc agcggtggtc ctccagtatc gattggtgct ggacaaatgg ctactgccgc
     3481 ttctccgtcg agtggtgata caacctcagc aagctcaagt aacgtacagg tttcgtcgat
     3541 gacagccgag caaatgagtt ctgttgcgga aggagcggtt gctcaagata acacatttgt
     3601 ggcggcagtc accatcgccc ctgcctctgg ggataactcg agcggacctt cgaacaatca
     3661 acaggcgcaa atgatgtcgc ttgcttcgaa cagtgcgcct ccgccaccat ctatggacag
     3721 cagcggtcct cggccagtcg atgtgccagt actaaatagt atgagttttc tgccccccgt
     3781 taatattatc gtgggcggtt tggttcgctt gactgtatct attcaataaa gggctagaag
     3841 catgaatcag aaaaaagtgg cagaagattt gagggccatt ccttttgcgg ctagacgcca
     3901 gcactttttg tctcagattg tggctcaaac tgaaaacgcg gttgcaattg tgcccgctca
     3961 ctctgagctg attcgaaatc acgatgtgca ttttaaattc agacaggata ccaacttcta
     4021 ttacttaact gggtttgagg aaccagatgc tatcgcagtt tttcgaaacg ttggtggcaa
     4081 gcgagaatat gttttgttcg t
//
LOCUS       DAOMXO010000135         4098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11821_length_4098_cov_4.656443, whole genome shotgun sequence.
ACCESSION   DAOMXO010000135 DAOMXO010000000
VERSION     DAOMXO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11821_length_4098_cov_4.656443"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLH57_03910"
     CDS             <1..474
                     /locus_tag="PLH57_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01785.1"
                     /translation="DFFQVANEVVDDAGSGLPGIDRTGTYFYNLLMSAGSLVLSPFLT
                     LIQLVISAFFIHFGVRLFMSTHEGVTPKYTTTLKILAYCSASMFLCVIPGVGIFLAFL
                     LWMVPAVVGLSTVYRSTGTRAVFAIATPMMLFGLVVLLLVFIFAVLGLGLLRLAI"
     gene            516..1136
                     /locus_tag="PLH57_03915"
     CDS             516..1136
                     /locus_tag="PLH57_03915"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRK01786.1"
                     /translation="MVNAIPRGAGWIEVVCGSMFSGKTEELIRRLKRAQIARVKVQCF
                     KPVIDDRYSKDHVMSHDATRISSIQIDRPWQILEQLGDNTRVIGIDEIQFFDDSIVEV
                     AQKLANRGVRVILAGLDQDYLGQPFGPMPRLLAIAEDVTKLSAICVQCGAPAHRTQRL
                     VSRAEQIAVGSKDMYEARCRICHDPSLGAESAQGTLNLAGPSHSAS"
     gene            1133..>1702
                     /locus_tag="PLH57_03920"
     CDS             1133..>1702
                     /locus_tag="PLH57_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRK01787.1"
                     /translation="MIKDRTYTNVQYRLSEIEHRYGSNVHILSDPFLLTHLAQLCSVN
                     TTQPIINQLVDTIYSNLVKVIINMEFPQKRVTVRSRMAEHHPEGLFESNLVDAEQPAV
                     CVNLARAGTFPSHICYNALNYVLNASVVRQDHISINRATDGQEQVVGVRVAGHKIGGS
                     IDNAIVLFPDPMGATGSTIISALDIYKKEV"
     assembly_gap    1703..1712
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1713..1959
                     /locus_tag="PLH57_03925"
     CDS             <1713..1959
                     /locus_tag="PLH57_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRK01788.1"
                     /translation="GTAKKFVAVHLIVTPEYLKRASERHPDLVIYAVRLDRGLSTKEV
                     LDSVPGTHWQKEVGLNSKQYIVPGGGGFGEILNNSFV"
     gene            1967..>2307
                     /locus_tag="PLH57_03930"
     CDS             1967..>2307
                     /locus_tag="PLH57_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRK01789.1"
                     /translation="MLLKDPKVLIPETKIMNRVHELASEIAFDYRGKEITAICVLKGS
                     FIFFADLIRNIDLPLNCEFMAVSSYGSKTTSSGEVKLTLDLTEPLEGKHVVVIEDIVD
                     TGLTMQYLVENL"
     assembly_gap    2308..2317
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2318..2498
                     /locus_tag="PLH57_03935"
     CDS             <2318..2498
                     /locus_tag="PLH57_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRK01790.1"
                     /translation="KPATLRTCALLFKPEALQCQLDLDYIGFKLGKEFVVGYGLDYAG
                     RYRQLPYIGALESEH"
     gene            2501..3574
                     /locus_tag="PLH57_03940"
     CDS             2501..3574
                     /locus_tag="PLH57_03940"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01791.1"
                     /translation="MKTITAKAPARIDLAGGTLDLWPLYLFFENTATINCAIDQIATV
                     EIKLGTESKIGETPSLEFASGDRGAKHKFKSFGELMESFQGTHKAVGLPPALWLHAKV
                     VRHFFAFWRRSAPLSLSTFADAPAGAGLGGSSALNIAMCKALQHVTNANYTDDEIIGV
                     ARDLETTVIEVPAGIQDYYSALHGGVQAIRMDAGRVHRKVFYKQSDFIQSNIVLCYSG
                     HSRNSGINNWAVYKGFIDKNPDIHQAFKEIVKATYRVEEALERLSFEKLVEGINGEWK
                     ARQLLAPTIATPDMLAAIEAAKSVGAVAAKVCGAGGGGCFIVVAPPEKRKPVVEKLQA
                     SGVKVIDFKVHAEGCRVVEATAE"
     gene            3586..>4098
                     /locus_tag="PLH57_03945"
     CDS             3586..>4098
                     /locus_tag="PLH57_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01792.1"
                     /translation="MVRPSPRDNVKRVFGLSLGGARSDRTCLTVIDFYQRDEKSNEKA
                     FVVDVFEGIGQSGELDGDEALLKIVNESLMERPATLLAVDAPLTLPPCFVGCADTCEG
                     YQKCKRPTVKWMRNQFLKARQKNRGIKGFTPYLQRPVDIYFRYQFTDKELFKDDTLGA
                     NYAPQAARMQY"
BASE COUNT         1060 a          861 c         1022 g         1135 t
ORIGIN      
        1 gatttttttc aggtagccaa tgaggtggtc gatgacgccg gttccggatt accgggaatt
       61 gatcgcactg gtacctattt ttataatctt ttaatgagcg ctggatcgtt agttctttcg
      121 ccttttttaa cgctcattca attagttatt tcggcctttt ttattcattt cggtgttcgt
      181 ttatttatga gtacccatga aggggttact cctaaatata cgaccactct caagatttta
      241 gcctactgct ctgcctcaat gttcctttgt gtcattccgg gtgtaggcat atttttggca
      301 tttcttttat ggatggtacc cgctgtggtc gggttaagca cggtgtatcg ttccactgga
      361 acgcgagcgg ttttcgcgat tgcgactccc atgatgctat ttggcctagt ggtgttactg
      421 ctcgtgttta ttttcgcagt tctgggactg ggtcttctaa gacttgccat ctagcggaaa
      481 ttcagtgata aatgcgactc gatgggggac ttttcatggt caatgctatc ccgcgcggag
      541 ccggttggat tgaagtagtc tgcggcagca tgtttagcgg aaaaacagag gaattgattc
      601 gaagattgaa acgcgcgcaa attgcgcggg ttaaagttca atgttttaaa ccagttatcg
      661 acgatcgata ttccaaagat catgtcatga gtcacgatgc aacgcgcatc tcgagtattc
      721 aaatcgaccg accatggcaa attctcgaac agctgggtga caacactcga gtcattggaa
      781 ttgatgaaat tcagtttttt gatgattcga tcgttgaggt cgcccagaaa ctcgcaaatc
      841 gtggagtccg ggtgatttta gcgggtttgg atcaagacta tttgggacaa cctttcggtc
      901 caatgccgag actattggca atcgccgaag acgtcaccaa attgtcggct atttgcgtac
      961 agtgtggtgc tcctgctcat cgaacccaac gcctcgtttc gcgggcggag caaatagctg
     1021 tgggaagcaa agacatgtac gaagcgcgat gtcgaatatg tcacgatccg tcattgggtg
     1081 ctgaatcggc ccagggaact ttgaatctgg ctggcccgag ccattccgca tcatgataaa
     1141 agaccgcaca tacacgaatg ttcagtaccg gctttccgaa attgaacatc gttacgggtc
     1201 aaacgttcat attctttctg atcccttcct actcactcat ttggcgcaac tatgttctgt
     1261 gaacacgacg cagccaatta ttaatcaact agtcgacaca atttattcaa acctcgtcaa
     1321 agttattatc aatatggagt ttcctcagaa acgggtgaca gtacgttcgc gaatggcgga
     1381 acaccacccg gaagggcttt ttgaatcaaa cttagttgat gcagagcagc ctgcggtgtg
     1441 tgtgaatttg gcgcgtgccg gtacttttcc ttctcatatt tgttataacg cattgaatta
     1501 tgtattgaac gcaagtgtag tgcgacaaga tcacatatca atcaatcggg ctacagatgg
     1561 gcaagagcag gtcgtaggag ttcgagttgc cgggcacaaa ataggcgggt cgatcgacaa
     1621 tgcaatcgta ttgtttccag accccatggg ggcgactggt agtacgatta tttcggccct
     1681 cgatatttat aaaaaagaag ttnnnnnnnn nnaggtaccg caaaaaaatt cgttgcagtg
     1741 cacctgatcg taacgccaga atacctgaag cgggcgtcgg agcgacatcc cgatttagtc
     1801 atttatgcag ttcggcttga tcgtgggctt tcaactaaag aagtcttaga cagtgtgccg
     1861 ggaacccact ggcaaaaaga agtcggcttg aattccaaac aatacatcgt accgggcggt
     1921 ggcggttttg gagagattct gaacaattcg tttgtgtgag gtgcttatgt tgttaaaaga
     1981 tccaaaagtt ttgattcctg aaactaaaat tatgaatcga gttcatgaac tcgcatcaga
     2041 aatcgcgttt gattatcgag gaaaagaaat caccgctatt tgtgttttga agggatcttt
     2101 cattttcttt gctgatctta ttcgaaacat cgatcttccg ttaaattgtg aatttatggc
     2161 ggtttctagt tacgggagta agacgacttc gtccggcgaa gttaaactga cgttggatct
     2221 cacagagcca cttgaaggca aacacgtagt cgtgatcgaa gatatcgtag acacgggcct
     2281 gaccatgcaa tatttggtcg aaaatctnnn nnnnnnnaaa gcccgccact ctgcgaacct
     2341 gtgcattgct ttttaaaccc gaagcccttc agtgtcagct ggaccttgat tatattggtt
     2401 tcaagctcgg aaaggaattt gttgtcggat acggacttga ttatgcgggc cgctatcgac
     2461 agctgcctta tattggcgca ttggagagtg agcactaaaa atgaagacta tcaccgccaa
     2521 ggccccagct cgcatcgatt tggctggtgg aacattggac ctttggccgc tttatttatt
     2581 tttcgaaaac actgcgacga ttaattgtgc gattgatcaa atagctactg tcgaaattaa
     2641 attggggact gaaagtaaaa ttggcgaaac tccttcgctc gaattcgcga gcggagaccg
     2701 aggagcgaaa cacaaattta aatcctttgg cgaactgatg gagtcatttc aaggcacgca
     2761 caaggccgtg ggattgcccc cggcattgtg gttgcatgct aaagtggttc gccatttttt
     2821 tgctttttgg cgccgatcag ctcctttgag tttaagtacc tttgctgatg caccagctgg
     2881 tgcgggtctc ggtggttcca gtgcgctgaa catagcaatg tgtaaggctc tgcaacatgt
     2941 gacgaatgca aattacaccg acgacgaaat cattggcgta gcgagggact tagagactac
     3001 agtcattgaa gtacccgctg gtattcaaga ctattattcc gctctacatg gcggagtaca
     3061 ggccattcga atggatgccg gcagagttca taggaaggtt ttctacaaac aatctgactt
     3121 cattcaaagt aacatagttc tctgttattc ggggcattcg cgaaactctg gcatcaataa
     3181 ctgggctgtc tataaagggt ttatcgacaa aaatcccgac attcatcaag cattcaaaga
     3241 gattgttaaa gctacttacc gtgtggaaga agcactagag cgactatctt tcgaaaaact
     3301 agttgaaggt attaacggcg agtggaaagc ccgccagtta cttgcgccca caattgcgac
     3361 gcctgatatg cttgcggcca tagaagcagc aaaatcagtt ggcgccgttg ctgcaaaagt
     3421 atgcggggcc ggcggtggtg ggtgtttcat tgttgttgcc ccccctgaaa aaagaaagcc
     3481 cgttgtggaa aagcttcagg catcgggagt caaagtaatt gattttaaag tacacgctga
     3541 aggatgtcgc gttgtcgaag cgaccgcaga gtgatcggag cttgattggt tcgtccgagt
     3601 ccacgggata acgtaaaacg agtatttgga ctaagtcttg ggggtgcgcg gtcagaccga
     3661 acttgtttga ctgtgataga cttttatcag cgtgacgaaa aatctaatga aaaggctttt
     3721 gtcgtcgatg tctttgaagg aattggtcaa agtggagaac tcgatggcga tgaggcatta
     3781 ttaaaaatcg tcaatgagtc gcttatggag cgaccggcga cgctccttgc tgtggatgcg
     3841 cctttgacac ttccaccgtg tttcgtgggc tgtgcagata cttgtgaagg atatcaaaag
     3901 tgtaagcgtc cgactgttaa atggatgcgg aatcaatttt taaaggcccg ccaaaaaaat
     3961 cggggtataa aaggatttac accgtatcta caacgacccg tggatatcta ttttcgttat
     4021 caatttaccg ataaagaact ttttaaagac gatacgcttg gcgccaacta cgcgccgcag
     4081 gctgctcgaa tgcagtat
//
LOCUS       DAOMXO010000136         4096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11831_length_4096_cov_6.750557, whole genome shotgun sequence.
ACCESSION   DAOMXO010000136 DAOMXO010000000
VERSION     DAOMXO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11831_length_4096_cov_6.750557"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLH57_03950"
     CDS             <1..201
                     /locus_tag="PLH57_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01793.1"
                     /translation="KNPAAKARKAAPKKAAARKAAPKTTNLGSPLSGFAGGLGAGALG
                     SAFGSSSSFGFGGGGRDYSAEE"
     gene            complement(271..>4096)
                     /locus_tag="PLH57_03955"
     CDS             complement(271..>4096)
                     /locus_tag="PLH57_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01794.1"
                     /translation="TKGGFSRFNLTVLATMFGAFGIVGCVQPELVDDAAVSTAPAVVT
                     PAFTIVSVPDLNSSSATGTFNFTYVPSDASVTCYVDADPAAPCISPHSFNALADGAHT
                     ANFSLNGGAATYTYTWTVDTAAPGAPAAVLASSTPTANSAATITIGSCAGISHIFVNE
                     GGVAPSAGAAGWQTCSTVAAAITYTLTTNGNRTLNVWAKDSANNVSATSTDLAVTFDN
                     VGPTIVITEPDNSDALKGGANFDVTWTATDTNMAANTITIDYSTDSGTNWTNIVTNTA
                     NDGTYTWAVPALNTANVQVRITAVDSLGNSDNENSTGNFIDSTAPTLTAAQMSLAAGA
                     ATTTSQYVAVDLAGSDNLTNITEFCLKYNSTTTPTAAASCWVPVTANPPGLVAAQTLT
                     LDDYSFGLGFVGGAYTVYAWLKDAAGNISTLSNAGAGTDAQDRDDITYSPGQAPTVVD
                     IAVVDSDPLPAPITSTDTAFELAGDFVYIHWDASDDGAAGSISIDLLYTTNDSTWTQF
                     ATGLAYNDSTNCTVAAGRTGCYRWALPGALANTYFRIRVKATDATSMVSYTNSNGLNV
                     WPPINYLAGNTEPGTGASASAAVFFNNTVTSSVPDVGSMVVASNGTIYFRDIYRGILR
                     ILPTDGVVSIYIPLNSAAASNNGDGGAATSATVKDPLKIALDFSDRLLIWDDDRIRRV
                     ETNGTINTLIGGGAAADSNGMAPTDVQFTAQNYATTFYAPFYILPNGDLYFASGTSTG
                     VGAAAMKYKLYDASSNTVSIFSISGTGDRQNVATPVADCFSYGFGVSFDAATSNVNYI
                     IGEVAHGATYGGCTLGGSGASGVRLDASGVSVGGVITNHPTTNSSCIKVTGRDGKLYC
                     VSRLLGRIQQYDDTARAWTTVIGTSTVGYCADGTAATSCNIEPHDLFVTQNGTMFFVD
                     RGKIRAVDTSGNVVTVAGQGFNYGDGGEALSARFGTIPDIALWDDAGTDKVILLDSFN
                     YRFREFAIGGTISTIAGNGTGGTPSTGAAATGQPLTIGHSGQIFDLFEADATGAIYFN
                     RTANYIAKMPRSGGTAGQWVNLVGGGATSYTTAVDTTLGANISLSGTTAYPAVIIGND
                     GTNILTAFGTYTSPSHVNAMVHAYAMSDGAITRGGGVIGAANSTFCAVGNAVSGCNVP
                     MAHGGLYRGSYWDPTAANWVIAHANYPTLPKTFPIGGNLGDLVTLPRSFSAITGYHPD
                     GLTHMLYYCSGGRLYKYNMLTTTETALTWPVASMSCAGKQLIYSPAADRNSLIFIYTQ
                     ANMYGIAEYLNP"
BASE COUNT         1048 a         1037 c         1056 g          955 t
ORIGIN      
        1 aaaaaccctg ccgctaaagc gcgcaaggct gcaccgaaaa aagccgctgc tcgcaaagct
       61 gcgcctaaaa ctacaaactt gggcagccct ctttcgggtt ttgctggtgg tctgggcgcg
      121 ggcgctttgg gaagcgcttt tggctcaagc agctcttttg ggtttggtgg cggcggccgc
      181 gattacagcg ctgaagagta gacgccgagt tctaagattt tccaatatat agaccccaag
      241 ctgttgaatt gcggcttggg gttttttaat ttaaggattc aaatactcgg caatgccgta
      301 catgttggcc tgagtgtaaa taaaaatcaa agaattgcgg tcggcggcgg gactgtaaat
      361 taactgtttt ccggcgcagc tcatcgaagc aaccggccag gtgagcgcgg tttctgttgt
      421 ggttaacatg ttgtacttat aaagacggcc tcccgaacaa tagtaaagca tgtgggtgag
      481 cccatctgga tgatacccgg taatcgcact aaacgaccgc ggtagagtta ccaaatcacc
      541 aagattaccc ccaattggaa aggttttcgg aagtgtagga taattagcgt gggcgatcac
      601 ccaattcgcg gcggttggat cccaataact tccgcgatac aagccaccgt gagccattgg
      661 tacattacaa ccggatactg catttccaac tgcgcagaat gttgaatttg cagctccgat
      721 aaccccccca cctcgggtta ttgcgccatc actcattgcg taagcatgca ccatagcgtt
      781 gacgtgactc gggctagtgt aggtgccgaa agcggtgagg atattggtgc cgtcgtttcc
      841 aatgatgact gcaggataag cggtcgttcc cgacagcgag atattcgcgc ccaaagtggt
      901 gtccactgca gtagtatacg aggttgcccc accacccact aaattgaccc attgaccggc
      961 ggtgcctccc gagcgtggca ttttcgcgat ataattggcg gttctattaa aatagatggc
     1021 tccagtagca tcggcttcga acaaatcaaa aatttggcct gaatgcccga tggtaagtgg
     1081 ctgaccggtc gcagccgctc ctgtactagg cgtgcctccg gtaccatttc cagcgattgt
     1141 cgaaatagta ccgccgattg cgaactccct gaagcggtag ttgaagctgt cgagcaaaat
     1201 aactttgtca gtaccagcat cgtcccagag cgcaatgtcc ggaatagttc caaaacgcgc
     1261 agaaagagcc tcaccgccgt cgccgtagtt aaaaccttgg ccggcaactg ttacaacgtt
     1321 accgctggtg tcgaccgctc tgatttttcc gcggtcaacg aaaaacattg taccattttg
     1381 cgttacaaaa agatcgtgcg gttcaatgtt acacgacgtt gccgctgtgc cgtcggcaca
     1441 gtatccgacc gtcgaagtgc caatgactgt ggtccacgct cgtgccgtgt cgtcgtactg
     1501 ttgaattcgg ccgaggaggc gggagacaca atagagtttg ccatcacgac cagttacttt
     1561 aatacatgaa gagttggtag ttgggtgatt tgtaataact ccgcccaccg acactcccga
     1621 tgcatctagc cgtactccgg aagcgccgct tcccccaaga gtacacccac cgtaggtcgc
     1681 gccgtgagca acttccccga taatgtaatt cacattagat gttgctgcgt caaacgaaac
     1741 cccaaagccg taggaaaaac aatcggccac aggagttgcg acattttggc gatcaccagt
     1801 gccagaaatc gaaaaaatac ttaccgtatt gctgctagca tcgtagagtt tatacttcat
     1861 tgccgccgcg ccaacaccag tactcgttcc ggaagcaaaa tatagatccc cgttcggtaa
     1921 tatgtaaaac ggggcgtaaa aagtagtggc ataattttgg gcggtaaatt gaacatcggt
     1981 aggcgccata ccattggaat cggccgcggc accaccgcca atcaaggtat tgatcgtacc
     2041 attggtttct acacgacgaa ttcgatcgtc atcccagatc agaagccgat cagaaaaatc
     2101 gagcgcaatt ttcaagggat ctttcaccgt cgcggaagtg gcggcccctc cgtcaccatt
     2161 gttggaggca gcagcactat ttagtggaat ataaatactc acaacgccgt cagtcggcaa
     2221 aatgcgcaat attccccgat aaatatcccg aaagtagatt gtgccattcg aagccacgac
     2281 catgctacca acgtcaggaa ccgaagatgt taccgtatta ttaaaaaaca ctgcggcaga
     2341 agcgctagca ccggtaccgg gttcagtatt cccggctaaa taattgattg gcggccatac
     2401 gtttaatccg ttggagttag tgtaactcac catcgaagtt gcatccgtgg ctttcacacg
     2461 aattctaaaa taagtgttgg caagagcgcc ggggagcgcc caacggtaac acccggttct
     2521 accggcggcg accgtacagt tcgtgctatc gttataagcc aaaccggtag cgaactgggt
     2581 ccacgtactg tcgttggtgg tgtacaacaa atcaatgctg atactacccg cagctccgtc
     2641 gtcgctggca tcccagtgaa tgtaaacgaa atctcccgca agttcgaatg cggtgtcagt
     2701 agaagtaatg ggagcaggga gcggatcgct atccaccact gcaatgtcga caacggtcgg
     2761 agcttgacct ggggaatagg tgatatcgtc tcgatcttga gcgtcagtac ctgcaccagc
     2821 attcgaaaga gtagaaatgt tacctgccgc atctttcagc caagcgtaaa ccgtgtacgc
     2881 accgccgacg aagcctaatc cgaaggaata gtcgtccaga gttaacgtct gggcggcaac
     2941 taatcccgga ggatttgcag ttactggtac ccaacagctg gcagcagcag ttggggtagt
     3001 tgtagagttg tacttaagac agaattccgt aatattcgtt aaattatcag aacccgctaa
     3061 atccacggct acatactgac tcgtcgttgt ggccgccccg gcggcgagcg acatttgagc
     3121 tgcagttaat gtaggggccg tagagtcgat aaaatttcca gtcgagtttt cgttatcgga
     3181 atttccaaga ctatccactg cggtaattcg cacttgaacg ttcgcggtat tcaacgcagg
     3241 tactgcccag gtataggtgc cgtcgttggc tgtattggtt acgatgttgg tccagttggt
     3301 gccactatcg gtcgaataat cgatggtgat cgtattagca gccatgttgg tatcagtagc
     3361 ggtccaagtc acatcaaagt tagcgccacc tttgagagcg tccgaattat ctggttcggt
     3421 tatcacgatc gtcggaccta cgttgtcaaa cgtcactgct aagtccgtag acgtggctga
     3481 tacgttgttc gcggagtctt ttgcccaaac attgagtgtg cgattgccgt tggtggttaa
     3541 ggtataggtg atcgcggcag ctaccgtgct acatgtttgc caaccagccg cgcccgcgct
     3601 cggtgcaacg ccaccttcat ttacaaatat atgggaaata cccgcgcaac taccaatcgt
     3661 aatcgtggcg gctgaattag cggtgggcgt tgatgaagca agaactgcgg caggggctcc
     3721 cggagcagcc gtgtcgacag tccaagtgta ggtgtaggta gcagctccgc catttaacga
     3781 aaaatttgca gtgtgcgccc catcggccag cgcattgaaa ctgtgtggtg aaatgcaagg
     3841 agcggctgga tcggcatcga cataacaggt aacgcttgcg tcactcggga cataagtaaa
     3901 attaaatgtc cctgtggcgg aactcgaatt taaatcgggc acactcacaa tagtaaacgc
     3961 tggagtcacc acggccggag cagttgatac cgccgcatcg tctaccaatt ctggttgcac
     4021 gcaacccacg atgccgaagg caccaaacat ggttgctaaa accgtcaaat taaaacgaga
     4081 aaaaccaccc ttagtc
//
LOCUS       DAOMXO010000137         4076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11913_length_4076_cov_4.928127, whole genome shotgun sequence.
ACCESSION   DAOMXO010000137 DAOMXO010000000
VERSION     DAOMXO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11913_length_4076_cov_4.928127"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..2940
                     /locus_tag="PLH57_03960"
     CDS             112..2940
                     /locus_tag="PLH57_03960"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRK01795.1"
                     /translation="MKQTAQYLKSQNADYIEELYARYLENPHSVDESWQYFFDGLVMG
                     EGAAKDDAEEHLSNGKSNGHANGNGGASGPQLVATAAPVTPLSSALSHEAKVAELIQA
                     YRELGRLLADTNPLESPTKSHELLDLKKFELSEADLERKFTAARLLGFTQPATLREIL
                     AMLHETYCGKIGVEYTHIEDPGSRHWLEDRMEKVRNRPQFDGAIKKQILKKLTEAECF
                     ERYLHTRFVAQKRFSVEGGDAIIPMLDRLAVRASALGASDMVMGMAHRGRLNVLTNIF
                     GKKYEDIFTEFEGTYQVDTSLGEGDVKYHMGYSADMQTPSGVIHLSLASNPSHLEFVN
                     PVVEGMARAKQTMKQDVAREKVVPVLIHGDAAFAGQGVVYETLQLSQLAGYATGGTIH
                     FVINNQVGFTTSPCDARSTTYSTDLAKMLESPIFHVNGDDAEACVYVTELAMDYRQKF
                     HKDVVIDLICYRKYGHNEGDEPGFTQPAMYRKIKDHRSPRETYAAELAARGDLSAADA
                     DMIVQQINGLLTEAHARAKSAAKAPHVSVFEGAWKGLKRASDEDIWVRTQTAVKETTL
                     KEIGKKMTEVPADFEVHPKLVRLLETRAKSVETGQNIDWGTGELLAYGSLLVEGRSIR
                     LSGQDCERGTFSHRHSVFANYSKDGKYSPLNHLKSGQGRFEVYNSNLSETGVLGFEYG
                     YSIADPSVLTIWEAQFGDFANGAQVIIDQFIASSEMKWQRMSGVTLLLPHGYEGQGPE
                     HSSARLERFLQLCAKNNMQVCNLTTPAQIFHALRRQLLRDFRKPLVVMSPKSLLRHPE
                     AVSKLADFTSAGFSEVIADSGADAERVGRVLFCSGKVFYDLQAARKTKGRSDVAIVRV
                     EQLYPWPTKQIESELKKYPNAKEVMWVQEEPRNMGAWLFVRDYLENNLVAGQRLSYVG
                     RGASAAPAVGSAKVHEKEQKALIEAALA"
     gene            3013..>4076
                     /gene="sucB"
                     /locus_tag="PLH57_03965"
     CDS             3013..>4076
                     /gene="sucB"
                     /locus_tag="PLH57_03965"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR01347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRK01796.1"
                     /translation="MNREIKAPSVGESITEVRIAGWKKQSGDAVKVGDVLLEIESDKA
                     TVEVVAEYNGAIQITRQAGETVKIGEVLGHIQEGAAGTVAAAKPAATQPSPTVATTMS
                     ASPAPMGAGATAAGVAMPLSPSVRKAVVEAGLDVGQIPGTGRGGRITNGDVIAYQETR
                     SSRGSTGSTPSTTTSIADPNARERRVPMTLLRQRIAERLVQAQHTAAILTTFNEIDMS
                     NTMALRSQHKDAFKSKHGVNLSFMSFFTRAVVEALKAVPAVNAFIDGKEIVYHDYYDI
                     GIAVGTERGLVVPVLREVGHMNFVDIEKGIGALALKARDGKLSIKELTGGTFTISNGG
                     TYGSLLSTPILNPPQSGILG"
BASE COUNT         1059 a          968 c         1107 g          942 t
ORIGIN      
        1 attttcctgg cttgcgcgcc tgatcgtcgt ggcgctatgt tgatttttct acagagaaac
       61 ttaagaaact gccgataaag aggcaacaca tagaggaaaa ggcattcgac catgaagcaa
      121 actgctcagt acctaaagtc gcaaaacgcg gattatattg aagagcttta tgcgaggtat
      181 ctcgaaaacc ctcactcggt tgacgaaagt tggcagtatt tctttgacgg gctcgtgatg
      241 ggcgaaggtg cggccaaaga cgacgccgaa gaacacctgt ccaacggaaa atccaatggt
      301 cacgccaatg gcaacggtgg tgcgagtgga ccccaattag tcgcgacagc tgcgccagtc
      361 accccgcttt cgagtgctct cagtcacgaa gcgaaagttg cagaattgat tcaagcctat
      421 cgcgaactcg ggcgattatt ggcggacacg aatccgctcg agtcgcctac caagagtcac
      481 gaactgttgg atctaaaaaa attcgaatta tctgaggccg atctggagcg caagtttacg
      541 gcggcgcgcc tcttggggtt tactcaaccc gctacgttgc gtgagattct tgcgatgttg
      601 catgagacct attgcggaaa aatcggtgtc gagtacaccc acattgaaga tccaggctcg
      661 cgtcattggt tagaagatcg catggaaaaa gtgcgcaatc gcccgcagtt tgacggtgcg
      721 attaaaaaac aaattttaaa aaaacttacg gaagcagaat gttttgagcg ctatctgcac
      781 acccggttcg tagcgcaaaa gagattttcg gtcgaaggtg gagacgcgat aatcccaatg
      841 cttgatcggc tcgcggttcg cgcgtcggcg cttggcgcga gtgatatggt tatgggaatg
      901 gcccatcgcg ggcggttgaa tgttcttacc aatattttcg ggaaaaaata cgaagacatt
      961 tttaccgaat ttgaaggtac ctaccaggta gatacgtcac tcggtgaagg cgacgtgaag
     1021 taccacatgg gctattcagc cgacatgcaa acgccgtcgg gagttattca cctttcgttg
     1081 gcctcaaatc cgagccacct tgaatttgtg aatccagtgg tagagggcat ggcacgcgct
     1141 aaacagacca tgaaacaaga cgtggcccgt gaaaaggtgg tacccgtgct tattcatggt
     1201 gatgctgcgt ttgcggggca aggcgtagtg tacgaaactc ttcagttgtc ccagttggcc
     1261 ggttatgcca ccggtggaac cattcacttt gtgatcaaca atcaagttgg gttcaccacc
     1321 tctccctgtg atgcgcgttc gacgacttat tccaccgatc tagcgaaaat gcttgagtcc
     1381 cccatattcc acgtaaatgg tgacgacgcc gaagcgtgtg tgtacgtaac cgaacttgcg
     1441 atggactatc gccagaaatt tcacaaagac gttgtgatcg accttatttg ttaccgcaaa
     1501 tatggccaca acgaaggaga tgagccagga ttcacgcaac cagcgatgta tcgtaaaatt
     1561 aaagatcatc gcagtccccg cgaaacctac gccgcggagc ttgccgcacg cggtgacttg
     1621 agcgcagccg atgcggacat gatcgtacag caaattaatg gattactgac cgaagcacat
     1681 gcccgagcga aatccgcagc caaggctccc catgtttcgg tattcgaagg tgcgtggaag
     1741 ggcctaaagc gcgcatcgga cgaagacatt tgggttcgaa ctcaaaccgc agtgaaagaa
     1801 acaacactca aagaaatcgg gaaaaaaatg actgaagttc ccgccgattt cgaagttcac
     1861 ccgaaattgg tgcgactctt ggaaactcgg gcgaaatcag ttgaaactgg tcagaatatc
     1921 gattggggta ctggcgaatt gctggcttat gggtctctgt tagtcgaagg acgttcgatt
     1981 cggctttccg ggcaagactg tgagcgtggt acgttcagcc atcgccactc ggttttcgca
     2041 aattactcaa aagatggaaa atattctccg ctcaatcacc tcaagtcggg gcaggggcga
     2101 tttgaagttt ataattcgaa cctctctgag acaggggttc ttggatttga atacgggtat
     2161 tcgattgccg atccgagtgt gctcacaatt tgggaagctc agtttggcga tttcgcaaat
     2221 ggcgctcaag taattatcga ccagtttatc gccagttcgg aaatgaaatg gcaacgcatg
     2281 agcggcgtta cgttattgct gccacatggt tacgaaggcc aggggcccga acactcaagt
     2341 gctcggttag aacgattttt gcagctttgt gctaaaaaca acatgcaggt ctgcaatcta
     2401 acgacgccag ctcaaatatt tcacgcgctt cgtcgccaat tgctccgtga ttttcgaaag
     2461 cccttagtcg tgatgtcgcc caagagttta ttgcggcatc ccgaagcggt tagtaagctt
     2521 gcggacttta cgtcggctgg gttttcggaa gtgatcgccg attcgggggc cgatgccgaa
     2581 cgagtggggc gtgtactgtt ttgttcgggt aaagttttct acgacctaca ggcagctcgc
     2641 aaaacaaaag ggcgcagtga cgtggccatt gttcgggtcg agcagcttta tccgtggccg
     2701 acaaaacaga tagagtcgga attaaaaaaa taccccaatg ctaaagaagt catgtgggtg
     2761 caagaggaac ctcgcaacat gggtgcttgg cttttcgtgc gtgactacct cgaaaacaac
     2821 ctagtcgcag ggcagcgtct ctcgtacgta gggcgcgggg cctcagcggc cccggcagtc
     2881 gggtctgcca aagtacacga gaaagaacaa aaagcgctca tcgaagccgc gttggcttaa
     2941 gattcgactt atttccttgc ttggcagcct cgagtaactc cgataaccta tgggcatcat
     3001 aggagaactc cagtgaatcg agagattaaa gcgccaagtg tgggtgaatc cattacagag
     3061 gttcgaattg caggttggaa aaaacaatct ggcgatgccg ttaaggtcgg tgatgttttg
     3121 ctggaaatcg aatccgacaa agcgacggtc gaggtagtgg ccgaatataa cggagccatt
     3181 cagatcaccc gccaggccgg cgagactgta aaaatcggag aagtcctggg gcacattcaa
     3241 gaaggggctg ctggaactgt cgcagcggct aaaccagctg caacacagcc gagcccgaca
     3301 gtggctacta cgatgagtgc tagtccagca cctatgggtg cgggcgctac tgctgcgggc
     3361 gttgcaatgc cgctttcgcc atccgtccgc aaagccgtag ttgaggcggg actcgacgtc
     3421 gggcaaattc ccggaaccgg gcgaggcggg cgaatcacca acggcgatgt tatcgcttat
     3481 caagaaacac gttcctctcg gggctctacg ggttcgacac catcgacaac aacctcgatt
     3541 gctgatccca atgcgcgaga gcggcgcgtt cccatgactt tacttcggca gcgcatcgct
     3601 gaacgtttgg tgcaggctca acatacggca gcaatactca cgacgttcaa cgaaattgat
     3661 atgagcaaca ccatggcttt gcgtagccaa cataaagacg cgtttaagag taaacatggt
     3721 gtgaatctta gttttatgtc tttttttact cgagcagttg tcgaggcact caaagctgtt
     3781 ccagctgtaa atgctttcat agacggcaaa gaaatcgttt atcacgacta ctacgatata
     3841 gggattgcgg ttggaaccga gcggggactg gtggttccag tgttgcgcga agttggtcac
     3901 atgaatttcg tagatataga aaaaggcatc ggtgcgttgg cgctgaaagc gcgagacgga
     3961 aaactctcga ttaaagaact cacgggaggg actttcacca tcagtaatgg tggtacttat
     4021 gggtctcttc tttcaactcc gattttgaat ccgcctcaaa gcgggattct tggcat
//
LOCUS       DAOMXO010000138         4076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11915_length_4076_cov_3.735389, whole genome shotgun sequence.
ACCESSION   DAOMXO010000138 DAOMXO010000000
VERSION     DAOMXO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11915_length_4076_cov_3.735389"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PLH57_03970"
     CDS             <1..636
                     /locus_tag="PLH57_03970"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRK01797.1"
                     /translation="LSTCLNVFEHFNPKLPESYLDSRYVFLANIDPDLQRSVYGQIKK
                     PVLVAADSMNFWITGKKQSLLQTLELVDLLTINEGEAFQLSGQNNILDAAKRIRAMGP
                     KILVVKQGEYGALLFTDQGIFSAPALPLATVKDPTGAGDTFAGGLMGYIAHEDAGDEI
                     FKDDAILRRAVIFGSVMASFTVEDFGFTNLLRTTHEQVQARYQEFLKMTRF"
     gene            complement(560..1735)
                     /locus_tag="PLH57_03975"
     CDS             complement(560..1735)
                     /locus_tag="PLH57_03975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK01798.1"
                     /translation="MNIAIIHDWLVSQRGGENVLDAICEIFPRADLFTMIYVPNSVSP
                     NIVRLNRQVSFLNRLPQAHRRYRHFLPLMPKAVERFDLSKYDLIISSSHCVAKGVLKA
                     PGAFHLSYVHAPMRYMWDRFDEYFSADRSGILTRTVARLMRSYFQRWDQNSASRVDAF
                     LANSNFIASKIKEYYGRDAQVVYPFCETENLGRARNPGKNFLMVTALVPYKRVDLAIR
                     AFDRLGLPLIIVGDGPDRSRLEKLAGKKTEFLGALKNDSLADLYSKCRALVFPGIEDF
                     GIVPIEAMAAGAPVIAFGAGGALETVTPQTGLFFTEPSEDSLIEAVQKFIATENQFSE
                     DACRKQGRKFNRTRFQLEFLEALVHALKKEHSTKIESFLKTLGNAPEPVRAWSLTSL"
     gene            complement(1738..2703)
                     /locus_tag="PLH57_03980"
     CDS             complement(1738..2703)
                     /locus_tag="PLH57_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK01799.1"
                     /translation="MVLEKNFRKYSHVVVTGGAGFIGSHLCEEYLKQGAAVTAIDNLV
                     TGSFKNIAKLEGTKGFRFIEHDISASMPDVGEFDLMLHFACPASPVDFAKMPLEILKV
                     DSLGTFNSLDAATKAKARYVLASTSEVYGDPLEHPQKESYWGNVNSIGDRSCYDEAKR
                     FAEATTVAYRRKLGTDSGMVRIFNTYGPRMRLNDGRVVPNFCGQALRGEALTVYGDGQ
                     QTRSFCYVTDLVDGIIRYAATDRTEPVNLGNPKEFKIRDFADFILKNIPQSNSKIESR
                     PLLHLDDPKQRRPDISRAKEWLGWEPRVSLEEGIKKTIEYFRTVL"
     gene            complement(2717..3832)
                     /locus_tag="PLH57_03985"
     CDS             complement(2717..3832)
                     /locus_tag="PLH57_03985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK01800.1"
                     /translation="MRVAIDARGISSQHRGVGRTTVNLLLGLSQRASDLEFVVFHTDP
                     YLPLLLKRAGGPFKGRFDWIQVRSPVGSVTERVEIPWLVRLHRVDLFHDIWGIGPNLR
                     RARSVATIHELEFLHFKDASRNSNRIRKVLRRSQLVVALTEAIAQEIESNLHISRPKI
                     RVVPGTVHPCFFERATEEEVRAMRGRFNLTGSYYLCVSGSRQSKNLNSLLEIATPAIP
                     VVLALQWEQIAHGQGYDLAAWKTAQESGAFRFLGTVEDFWLRALYAKAEGLIIPSFYE
                     GFSLAAVEGLACSCLPVLSDIPSHRNLFGDTRGLEALFFDPKVQGDLRAATERVRIAG
                     NSLKTVVLEKFRELCHRYAVNDVADRMQQVYREAAEL"
BASE COUNT         1110 a         1001 c          894 g         1071 t
ORIGIN      
        1 ttgtccactt gcctcaatgt ttttgaacat tttaatccga aattgccgga aagctatctt
       61 gattctcgtt atgtctttct tgcaaacatt gatccggatt tacaacgctc ggtttatggc
      121 caaatcaaga aaccagtttt ggttgctgct gattccatga atttttggat aactgggaaa
      181 aagcagtcct tactgcaaac ccttgaattg gtagatctac ttacgattaa cgagggcgaa
      241 gcgtttcagt tatctggtca aaataacatt ctcgatgccg caaagagaat tcgagcaatg
      301 ggaccaaaaa ttctcgtggt aaaacagggg gagtatggcg cgttactttt tacagaccag
      361 ggaatatttt ccgcacccgc gctacctctc gctaccgtaa aagatcccac tggcgccggt
      421 gacacatttg ccggaggcct catgggttac attgcgcacg aagacgccgg cgatgaaatt
      481 tttaaagacg acgccattct tcgcagagcg gtcattttcg gaagtgtaat ggcaagtttt
      541 actgtggaag attttggttt tacaaacttg ttaaggacca cgcacgaaca ggttcaggcg
      601 cgttaccaag agtttttaaa aatgactcga ttttagtgga gtgctctttt ttgagagcgt
      661 gcactaaagc ttctaggaac tctaactgaa acctggtgcg attgaatttt cgcccttgtt
      721 ttcggcaagc atcttctgaa aattggtttt cggtagcgat gaatttttga actgcttcga
      781 tcagactgtc ttcgctgggt tccgtaaaaa acagccccgt ttgaggagta actgtttcga
      841 gagctcctcc ggcgccaaac gctataactg gcgctcccgc tgccattgct tcaattggca
      901 cgataccaaa gtcttcgatt cccggaaaaa ctaacgcccg acattttgaa tataaatctg
      961 cgagagagtc atttttcaag gcacctagaa actctgtttt ctttccagct agtttttcta
     1021 aacgcgaacg atcagggccg tcccctacaa taattagcgg caatccaagt cgatcaaaag
     1081 cacgtatcgc gaggtctact cgtttgtagg gtacaagagc ggtgaccatt aaaaaatttt
     1141 ttccggggtt tcgagcacgc cctagatttt cagtttcaca gaagggataa acaacttgag
     1201 cgtctcttcc gtagtattct ttaattttac tagcgataaa attcgaatta gcaagaaacg
     1261 cgtcgacccg tgaggcggag ttttgatccc aacgttgaaa gtaacttcgc attaaccgag
     1321 ccacggttct ggttaaaatc cctgaacgat ccgcggaaaa atactcatca aaacggtccc
     1381 acatgtatcg catgggcgca tgcacataac ttagatgaaa tgcaccggga gccttaagca
     1441 ccccttttgc aacgcaatga gaactagaaa taatgagatc gtatttcgat agatcaaatc
     1501 gttctaccgc cttcggcatg agtggaagaa aatggcggta acggcgatga gcctgaggaa
     1561 gtcgatttaa gaatgatact tggcgattta gccgaacaat attgggagac actgaattcg
     1621 gaacgtaaat catggtgaac aagtcggcgc gtggaaatat ttcacaaatg gcgtcgagca
     1681 cattttcacc gccacgctgg cttacgagcc aatcatgtat aatcgctata ttcattgtta
     1741 gagaacagtt ctgaaatatt cgattgtttt cttgatgcct tcttcgagcg ataccctggg
     1801 ctcccaaccc aaccattcct ttgcacgaga aatgtcggga cgccgctgtt ttggatcgtc
     1861 aagatgaagc aagggacgag actcaatttt actgttactc tgaggaatat tcttcaatat
     1921 gaaatccgca aaatcgcgta ttttgaattc cttgggattt cctagattaa ccggctcagt
     1981 gcggtcagtg gccgcataac gaataattcc gtcgaccaaa tctgtcacat aacaaaatga
     2041 gcgtgtttgt tgcccgtcgc cataaactgt caatgcttcg ccccgtaacg cttggccgca
     2101 aaaatttgga acaacccgac cgtcattaag tcgcatgcga ggcccatagg tattgaaaat
     2161 tcgcaccatt cccgagtcgg tccctaattt tcgacgatag gccacggtag tcgcttcagc
     2221 gaaccgcttc gcttcgtcat aacacgaacg atcgccgatt gagttcacat taccccagta
     2281 gctttctttc tgcggatgtt ccaatggatc accgtaaacc tcagaagtac ttgctaaaac
     2341 gtaacgagct tttgccttgg tagcggcgtc taaactattg aaagttccca gcgaatctac
     2401 ttttaaaatt tcgagtggca tctttgcaaa atctacaggg ctcgcaggac aagcaaaatg
     2461 caacattaaa tcgaattcac cgacgtccgg cattgaagcc gaaatatcat gctctataaa
     2521 acgaaaaccc ttggtcccct cgagttttgc gatattttta aacgaaccgg ttaccaaatt
     2581 atctatcgcc gtgaccgctg ccccttgctt gagatattct tcgcagagat gggaaccaat
     2641 gaaccccgct cctccggtga ctactacgtg ggaatatttt cgaaaattct tttccaggac
     2701 catctacacc aatcctctaa agctctgcgg cttctcggta aacttgctgc attcgatcag
     2761 caacatcgtt gaccgcgtaa cgatgacaaa gttcgcgaaa tttttcaaga accacggttt
     2821 taagcgaatt tcctgctatt cgcacgcgct cggttgccgc tcgtaaatcc ccttgcacct
     2881 tggggtcaaa aaacaatgct tcgagccctc ttgtatctcc gaaaagatta cggtgactag
     2941 gaatatctga caaaaccggc aaacaagagc atgcaagtcc ttcgactgcg gcgagactaa
     3001 acccttcata aaaagaggga attataagtc cctcggcttt cgcgtacaaa gcgcgcaacc
     3061 aaaaatcttc aaccgtaccc aaaaaccgaa atgctcctga ttcctgggca gttttccatg
     3121 cggcaagatc gtatccctga ccatgcgcta tttgttccca ctgaagagct agaactactg
     3181 gtatcgctgg ggtggcgatc tcaagcaacg aattcagatt tttgctctgg cgactgcccg
     3241 aaacacaaag atagtaggac cctgtcaggt taaagcgccc ccgcatggct cgtacctctt
     3301 cctcagtcgc acgttcaaaa aaacacggat gaacagttcc gggtacgacc cgaatcttcg
     3361 gtcgagaaat atgaagattt gattcaattt cttgagcaat tgcttcggta agcgccacca
     3421 ccaattgaga cctacgtaaa acttttcgta tgcgatttga attgcgggac gcgtctttaa
     3481 aatgcaaaaa ctcgagctcg tgaatagtgg ccactgaacg ggctcgacgt aaattgggcc
     3541 cgatgcccca aatgtcatga aatagatcca cccggtgcaa tcgcaccaac cacggtattt
     3601 cgactctttc agtgaccgac cctacgggac tccggacttg aatccaatcg aacctaccct
     3661 tgaagggtcc accagcgcgc ttcagtaaaa gcgggagata gggatctgtg tgaaacacta
     3721 cgaactcgag atcggatgcc ctttgactga ggcccagcaa tagatttacg gtggtacgcc
     3781 ccacaccacg atgttgcgac gaaattcctc gggcatctat agcgactcgc atggccttat
     3841 cctctcacgc ccgatgcgct ttcacacaaa attttaatct ggtacaacca ccaacggcac
     3901 ggtaaaaatg tgttgtgcga gcattcgtag gttcaaattc gctgaatgtg ccgaaagtaa
     3961 tattggcttt gttgctaatg ttcctttttt tacagttcgc aagcgccgcg gaaccgccag
     4021 tttcgaaatc taattttcaa tcgccaatcg ctccggtcaa gcttttcgag ccagcc
//
LOCUS       DAOMXO010000139         4074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_11924_length_4074_cov_3.694949, whole genome shotgun sequence.
ACCESSION   DAOMXO010000139 DAOMXO010000000
VERSION     DAOMXO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11924_length_4074_cov_3.694949"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLH57_03990"
     CDS             complement(<1..444)
                     /locus_tag="PLH57_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRK01801.1"
                     /translation="MDKIVCVGKNYAAHVLEMGGNVSEKPVLFIKPPSVLRQVENSEI
                     ASVALPKNRGAVHYETEIVLMLGEKNQIKAVTLGLDMTLRDQQANLKKAGQPWEIAKV
                     FSNSAIVGPFIPIEEFADFATSEFQMKLDGVPRQKAQSTQMTLSPL"
     gene            complement(463..1869)
                     /gene="fumC"
                     /locus_tag="PLH57_03995"
     CDS             complement(463..1869)
                     /gene="fumC"
                     /locus_tag="PLH57_03995"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRK01802.1"
                     /translation="MKNAELKVRVETDTMGEMKVASDRYWGAQTQRSLLNFEVGDDRM
                     PRPMIRALGILKKAAALVNHDLGKLPKDKLDAIVKAADEVIAGTLDDHFPLVVWQTGS
                     GTQTNMNANEVISNRAIEILGGEMGSKKPIHPNDHVNMSQSSNDSFPTAMHIAAVEEI
                     EDRLLPALKKFHTALQMKEKKFEKIIKVGRTHLMDATPLTLGQEFSGYRTQIEIGIER
                     VEQTLERLRALAQGGTAVGTGLNTHPEFADRVAKKISEITKKKFLSAPNKFEALAAHD
                     ALVFAHGALKTLACSFMKIANDIRWLGSGPRCGLQELALPENEPGSSIMPGKVNPTQS
                     ESMTMVCCQVLGNDTAVNVAASNGHFELNVFKPVIIHNFLHSTRLLADSANGFVDHCL
                     EGLKPNEAVIAKHLENTLMLVTALNPHIGYDNAAKIAKTAHKEGTTLKEAALKLGLLN
                     EKQFAEWVRPEDMISPRE"
     gene            complement(1983..3065)
                     /locus_tag="PLH57_04000"
     CDS             complement(1983..3065)
                     /locus_tag="PLH57_04000"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HRK01803.1"
                     /translation="MIRNVVWAGVGLLVVSKPVFGACTINGEVVPCSRLLTEFSGVLA
                     LTVLAVVVILASCVSIILKIRGRIQNYRRVNSQLIRQNLPEANFKNAEAESRKNASHA
                     TPIGREEIRQKISETYGNLIQAHDRENPRGVIVLTTLALGISAIILVSSINSSLLLMK
                     GKKASGTVVMVKKSHGSFKANKKSGYIIVKFTSEDGLTQEFRNSVYNSASYVPGQQVQ
                     VIYNPTDSTDARLDSFYELWRTPAALSLFVIPLWGSCVAVIRKRKKNKSDREWLTKFG
                     NSVSATVIAINQSVVVNGKSYFTIEYQWTDLNTGDQRTARSEVIPFNPSVSGYVTIGQ
                     KIEVLVDPNNPSRTYFDIQTILNRSA"
     gene            complement(3239..3315)
                     /locus_tag="PLH57_04005"
     tRNA            complement(3239..3315)
                     /locus_tag="PLH57_04005"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3279..3281),aa:Ile,seq:cat)
     gene            complement(3306..3806)
                     /locus_tag="PLH57_04010"
     CDS             complement(3306..3806)
                     /locus_tag="PLH57_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01804.1"
                     /translation="MGFVWGSVVVLIFASLSVEAKVPELGKKKEPVLKASHRARSSEQ
                     KAKDQQRIEQKRKAAKKNQVVSRPVQNDSVQNECQRIIQKNPALFGGHLDPQSGSALE
                     PLEVLEKLKKYQKYLWDEPGMEARYYLEFPSDKLEDVPVETVAAALLWNSRVSKIEAS
                     VANAGP"
     gene            complement(3831..>4074)
                     /locus_tag="PLH57_04015"
     CDS             complement(3831..>4074)
                     /locus_tag="PLH57_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRK01805.1"
                     /translation="VMNINLSEQTRKVLATLTPREEKVLRMRFGIGEKSDHTLEEVGQ
                     DFFVTRERIRQIEAKALRKLRHPSRAKLLKAFVDGS"
BASE COUNT         1006 a          999 c          863 g         1206 t
ORIGIN      
        1 taggggcgac aaagtcattt gagtgctctg agctttttgt cttggaacac catctaactt
       61 catctgaaat tcggaggtcg caaaatctgc aaattcttcg attggaataa acggaccgac
      121 gattgccgag ttcgaaaata ctttggctat ttcccacggc tgtcccgcct tttttaagtt
      181 cgcttgctgg tcgcgtaagg tcatgtcaag accgagggtt accgccttaa tctgattttt
      241 ttcccccaac attaatacta tctcagtttc gtagtgaacc gctcctcggt tttttggtaa
      301 agccacacta gcgatttcag agttttctac ctgtcgcaaa acgctcgggg gctttataaa
      361 cagtacgggt ttttcgctta cgttccctcc catttcgaga acatgagcag cgtaattttt
      421 tccgacgcaa actattttat ccatagtaac ttcgatctca atctattcgc gcggagaaat
      481 catatcttct ggtcgtaccc attcagcaaa ttgtttttca ttaagaagtc ccaatttcaa
      541 agcggcttct tttaacgtgg tcccttcttt gtgggcagtt ttcgcaattt ttgccgcatt
      601 gtcgtaacca atgtggggat tcaatgcagt aacgagcatt agtgtgtttt ctaaatgttt
      661 cgcaataacg gcttcatttg gctttagccc ttctaaacag tggtcaacaa atccattcgc
      721 cgaatctgcg agcaggcggg tagaatgtaa aaaattatga ataattaccg gtttaaatac
      781 gttcagctca aagtgcccat tagaagctgc gacgttcacc gcggtgtcgt ttcctaagac
      841 ctgacaacat accattgtca tagattcact ttgcgtcggg ttcactttgc ccggcataat
      901 ggaagaacca ggttcgtttt ctgggagtgc caactcttga agaccacaac gtggtccact
      961 tcccaaccaa cgaatgtcat tagcaatctt catgaaactg caagccaggg ttttcagcgc
     1021 accatgcgca aacaccaatg catcatgcgc ggccagtgct tcgaacttat tgggcgcaga
     1081 caaaaatttc ttttttgtga tttcagaaat tttctttgcc acacgatctg caaactctgg
     1141 atgagtgtta agtccagtgc ccacggccgt accgccttga gccagcgctc gcaatcgctc
     1201 tagcgtttgc tccactcttt ctatgccaat ttcaatttgt gtgcgatagc cggaaaattc
     1261 ctggccgaga gtgaggggcg tagcatccat caagtgcgtt cggccgactt taataatttt
     1321 ttcgaatttc ttttccttca tctgcagtgc ggtgtgaaac tttttgagtg cgggcagcag
     1381 tcgatcttct atttcttcaa ctgcggcaat gtgcatcgct gttggaaacg aatcattgga
     1441 gctctgactc atgttcacat gatcgttcgg atggattggt ttcttggaac ccatctcacc
     1501 acccagaatt tcaatcgccc ggttcgatat aacttcatta gcgttcatat tggtctgagt
     1561 ccccgaaccg gtttgccaca cgacgagtgg gaagtgatca tcgagcgtac ctgcaatcac
     1621 ttcgtcggcg gctttcacaa tggcatcgag tttgtctttc ggaagttttc caagatcatg
     1681 attcactaag gcagcagctt tttttaaaat tcccaatgcg cgaatcatcg gtcgtggcat
     1741 gcgatcgtcg cccacttcga aatttaagag ggagcgttgg gtttgcgccc cccaatatct
     1801 gtcactcgct actttcattt cgcccatggt gtcagtttca actcgaactt ttagctcagc
     1861 gtttttcatg accccgatac taaaagcgaa tatacgtttt gaaaaggagg aaggcggctt
     1921 acgaataaac actgccttca aatcggtttt cagcagttct aaaggctaga tgcgcttcga
     1981 aatcaagcag acctatttaa aatagtctga atatcgaaat aggtgcgaga cgggttattc
     2041 ggatcaacta agacttctat tttttgacca attgttacgt atcctgaaac tgacggattg
     2101 aaaggaatta cctcgctacg tgcggtgcgt tgatcgcctg tatttaaatc ggtccattgg
     2161 tactcgatag tgaaatatga ttttccgttt accacgacac tttggttaat agcgattaca
     2221 gtagcactca ccgaattacc aaactttgtt aaccattcgc gatcgctttt gttcttttta
     2281 cgttttcgta tgaccgccac acaggacccc cagagaggaa ttacaaacaa gctaagtgcc
     2341 gctggagtcc tccatagctc atagaaacta tctaacctag catcggtcga atcggtagga
     2401 ttgtaaatca cctgcacttg ttgacctgga acataactag ccgaattata aacagagttt
     2461 cgaaattctt gcgtcagacc gtcctctgaa gtgaatttta caattatata gccactcttc
     2521 ttgttcgctt tgaaactccc gtgcgatttt ttgaccatta ctactgtgcc gctggctttt
     2581 tttcccttca tcagtaacag ggaggagttt atcgatgaaa ccaaaattat agccgatata
     2641 cccagtgcca atgtggtcaa cacaatgacg cctcgcggat tctctcggtc gtgtgcttga
     2701 attaaattac cgtaggtctc tgagattttc tgccgaattt cttcgcgccc aatcggagtt
     2761 gcatgggatg cattctttcg actctccgcc tctgcgttct taaaattagc ttctggtaaa
     2821 ttctggcgaa ttagctggct attcacccgc ctataatttt gaattctccc tctaattttt
     2881 aaaataatag atacacatga agccaaaatc acaacaactg ccagaactgt gagagcgagt
     2941 accccactaa attccgtaag cagcctcgag caaggaacta cttctccatt aatggtgcat
     3001 gccccaaata ctggtttcga aacaactaac aggccaacac ccgcccaaac gacatttcgt
     3061 atcatattta tgcttcggct ataaaaggag gcttgtttag attctatccg aatggggccg
     3121 gggtgagttt ttgcctgcga accaatgcag atttttcccc tgaaagaact acgtgcattg
     3181 gcttgtttgg ctgctcgaac tcacgacccc ctcgccgtgg ttcgaacagc ttgacaagtg
     3241 gtgggcccac caggactcga acctgggacc acccggttat gagccggaag ctctaaccaa
     3301 ctgagctatg ggcccgcgtt agctactgaa gcttcgattt tagaaacacg actgttccat
     3361 aaaagagccg cagctacggt ttcgacgggg acatcttcta gtttgtccga cggaaactca
     3421 agataatatc tagcttccat tccgggttcg tcccacaaat atttttgata tttctttaat
     3481 ttttctaaaa cctctaacgg ctctaaggct gacccggact gtgggtctaa atgcccccca
     3541 aaaagcgcag ggtttttctg aatgatccgt tgacactcat tttgcacgct atcattttgc
     3601 acaggccttg aaacaacctg attttttttg gcggcctttc gcttctgttc gattctttgt
     3661 tggtccttgg ccttctgctc ggaagagcgg gcacggtggg acgccttcaa gacgggttct
     3721 ttctttttgc caagctcagg gacctttgcc tcgaccgaaa gcgacgcaaa tatcaggacc
     3781 acgactgagc cccaaacaaa acccatattc gctcccccaa ggttgaaaaa tcagctgccg
     3841 tccacaaaag ctttgagcaa cttcgctctc gacggatgac gaagttttcg caatgccttt
     3901 gcttcgatct ggcgaatacg ttcgcgcgtt acgaagaaat cctgtccgac ttcttctaaa
     3961 gtatgatcgg atttttcccc gattccgaaa cgcattcgta atactttttc ttcacgtggt
     4021 gtgagcgttg caagcacttt tcgtgtttgc tcagacaggt taatgttcat tacc
//
LOCUS       DAOMXO010000140         4014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12182_length_4014_cov_4.077797, whole genome shotgun sequence.
ACCESSION   DAOMXO010000140 DAOMXO010000000
VERSION     DAOMXO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12182_length_4014_cov_4.077797"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /gene="glmU"
                     /locus_tag="PLH57_04020"
     CDS             complement(<1..1305)
                     /gene="glmU"
                     /locus_tag="PLH57_04020"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRK01806.1"
                     /translation="MRLSTVKKIDSLEIVVLAAGKGTRMKSEVPKVLQPICGHPILEI
                     VLHSIEGGLQNLGREFQINKSTINLVVGHGRDQVKAMVAGLEQNKRICSTVNFSVQEQ
                     QLGTGHAVKVALSNSIIYPKAGSVDLLMVVSGDLPLLTPDSLAQFVRAHLAMRSSASL
                     MSARLTNAGAYGRVLRRGGTFYGVVEAKDATAKQLSIQEFNGGVYVFDRAVLQDALGK
                     IDSKNAAKEFYLPDVFTVAMKKRKKILAHCVDDPNIVEGVNTMVDLANTERELYLRIA
                     RQHMLNGVRLSYPETIRISPFVKLADNVSIDAFSRVAGYTSVGAGSRIGSFCDLSQVE
                     IGSCVDIRNSVVAQNSKIQDGAIVGPMANLRPATEIASGAKIGNFVELKKSVIGPRTN
                     VSHLSYVGDAEIGSDSNVGCGFVTCNYDGNEKHRSIIGSRVFI"
     gene            1445..1738
                     /locus_tag="PLH57_04025"
     CDS             1445..1738
                     /locus_tag="PLH57_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01807.1"
                     /translation="MKLRLGLILLAVASVWFSPNAQASNTSVIGAPVDSFALCQKNLK
                     IIVDSSVEEGETVVETSCKRFEILNPETGDLEVRFQPAALIEGSPISSDEYQN"
     gene            complement(1713..2921)
                     /locus_tag="PLH57_04030"
     CDS             complement(1713..2921)
                     /locus_tag="PLH57_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK01808.1"
                     /translation="MKNRQILLVSYFFEPLQTVAAERWARFCAGLKKRGLDLHVLAGP
                     WKSGDTDSSRDIEYLDDPVSEDSGKYKTGFAPTSGFRSTMRKLIPLFLLIDGKWFWSL
                     RVFLKVIQRRHSTSVLIVTGTPWSSVVAAALAARVSSLPYIVDFRDLWATEPFLRSQS
                     ALARRYFAWLERWVVRGAMSILTVNDPLTQYFQNLDTTKKVVCIPNGFSGELHYNEVT
                     FNSMATAQTHLLYGGSISGHSGVAEFLKAFDLAQVPVDLAFMGTDFLGVLPHPHAAHI
                     PQVSGREAEMEMARAGVLLLTLHRKSVHYVTGKLMSYLKAGRPILYWGPLESPAAKIV
                     RECGVGWVIANDDLEGLKRALTEINERFHDRHSFAFAPNLTQIREYSVDRMVERIERE
                     VRLSFGIRRN"
     gene            3099..3458
                     /locus_tag="PLH57_04035"
     CDS             3099..3458
                     /locus_tag="PLH57_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRK01809.1"
                     /translation="MDQKSLTKFKKMLLEERQNVLNHSKKDRNDIIVDSDDLADETDL
                     AASEVSQALAFSLRDRERLLLSKIDQALEKIEEGTFGICETCEEPIEIRRLEARPVSN
                     LCLSCKELEEHKEKIFA"
     gene            3690..>4014
                     /locus_tag="PLH57_04040"
     CDS             3690..>4014
                     /locus_tag="PLH57_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRK01810.1"
                     /translation="MVTNYDGEQIEVPATLPMLPVRDIVVFPYMIIPLFVGRESSIKA
                     VEESLARNDRLILLASQKDISDEHPNPDGIYQIGTVAMIMRMRKLPDGRIKILTQGLC
                     KAHIDE"
BASE COUNT         1069 a         1046 c          891 g         1008 t
ORIGIN      
        1 tataaaaacg cgacttccaa ttatcgaacg gtgtttttca ttgccgtcgt aattacaagt
       61 cacgaatccg caaccaacat ttgaatccga tccgatttcc gcgtccccga cgtagctcaa
      121 atgggaaaca tttgttcgtg gtccgattac cgattttttt aattcaacaa aatttccaat
      181 ttttgcaccg gaagcaattt cagtagcggg tcgaagattg gccatgggtc caacaatggc
      241 gccatcttga attttcgagt tttgcgccac cacactgttt cggatgtcga cgcaactacc
      301 aatttctact tggctgagat cacaaaagct accaattcga ctaccagccc caaccgaagt
      361 gtagcctgcg actcgtgaaa acgcatcgat actcacattg tctgcgagtt ttacgaacgg
      421 cgagattcga atggtttctg gataagacaa acgaactccg ttaagcatgt gctgtcgcgc
      481 aatccgtagg tataattcgc gttcagtatt cgccaaatca accatcgtat tgacgccttc
      541 cacaatatta ggatcgtcaa cacagtgagc gagaattttt tttcgttttt tcatcgccac
      601 tgtaaacacg tcggggagat aaaattcctt agcagcattc tttgaatcta tttttccgag
      661 cgcgtcttga agtacagcgc gatcaaacac gtagacgcca ccgttgaatt cctgaatcga
      721 tagttgtttt gcagtggcat ctttggcttc aaccacaccg taaaaggttc cacctcgtcg
      781 caacacccgg ccataagcgc ccgcgttcgt tagccgggca gacatcaagg aggcggacga
      841 acgcattgcc aagtgggctc gcacgaactg cgctaaagaa tctggcgtaa gcagtggtag
      901 atcaccactc actaccatta aaagatccac cgaaccggcc ttggggtaaa ttatagagtt
      961 cgagagcgca acttttacag cgtgaccggt tcctagttgt tgttcctgta cactaaagtt
     1021 tacggtggaa caaattcgct tattttgctc aagtccggcg accattgcct taacctggtc
     1081 gcggccgtgg cccaccacta aatttatcgt acttttgttt atttgaaatt cacgaccgag
     1141 gttttgcagc cccccttcga tcgaatgcag tacgatctca agaattggat gaccacatat
     1201 cggctgcagt acttttggaa cttcgctttt catgcgcgta cccttacccg ctgccagtac
     1261 gacaatctcc aaactgtcta ttttttttac agtcgataag cgcatgagtg gatccctctc
     1321 tcagaggtct tcactatacc cctgatattc ttagtttttc aaccaaacac ccgcctggcc
     1381 cctgcgttgc attctaatcg ggtaagaaca cccttgtggt tgctgttgtt gctaggagct
     1441 gaagttgaag ttgcgattgg gtctcatatt actggctgtt gctagcgttt ggttttcacc
     1501 gaacgcccaa gcgtcgaaca cctcggtgat cggcgcccca gtagattcgt tcgcactctg
     1561 tcaaaaaaac ctaaaaataa tcgtcgatag ctctgtggaa gagggcgaaa ccgtggttga
     1621 aacctcgtgc aagcgtttcg aaattttgaa tccagagacc ggcgatctcg aggtccgctt
     1681 tcaaccggcg gctttgatcg agggatcccc tatcagttcc gacgaatacc aaaactaagt
     1741 cgcacttccc tttcaattct ttcaaccatg cgatccacgg aatattcgcg gatctgcgtt
     1801 aaattcggag caaacgcaaa cgaatgacga tcatggaacc gttcatttat ctcggtaagc
     1861 gctcttttta acccctctaa atcatcgttg gcgatcaccc agcctacacc gcattctcta
     1921 acgatcttgg cggcggggct ctcaaggggc ccccaataca aaattggtcg cccggccttg
     1981 aggtaggaca taagtttgcc cgttacatag tgaacgcttt ttcggtgcag ggttagaagc
     2041 aatacgcctg ctctcgccat ttccatttct gcttcgcgcc cggaaacctg aggtatgtgg
     2101 gctgcgtgag gatggggaag cacccccagg aaatctgtgc ccataaacgc caagtccaca
     2161 ggaacctgcg ccagatcgaa cgccttgaga aactccgcca ctcctgagtg ccccgaaatg
     2221 ctccccccgt agagcaagtg agtctgggcg gtagccatac tattaaaggt gacctcatta
     2281 taatggagct ctccactaaa accgtttgga atgcagacga ctttcttggt ggtatcgagg
     2341 ttttgaaaat actgcgttaa cggatcgttc accgttaaga tactcatggc tccacgcacg
     2401 acccaacgtt caagccaagc aaagtaccgc cgcgctaatg ccgactgcga ccttaaaaaa
     2461 ggttcggtcg cccacagatc tcgaaaatct acgatatatg gcagactcga tactctagca
     2521 gccagcgcgg ccgccactac cgacgaccaa ggcgtacccg taacaattaa aacgctcgtg
     2581 gagtggcgac gctgaataac ttttaagaaa acgcgcagac tccaaaacca cttgccatcg
     2641 atcaacagaa aaagcgggat caactttctc atcgtacttc gaaaacccga ggtcggcgca
     2701 aaaccggttt tatacttacc gctgtcttct gagactgggt cgtccaaata ctctatatct
     2761 cgcgaagaat cggtatcgcc acttttccat ggaccagcca aaacgtgtag atccagccct
     2821 cgttttttga gtccagcgca aaagcgggcc caacgttctg cggccaccgt ttgaagtggt
     2881 tcaaaaaaat aggagacgag cagtatttgc cgatttttca tattcacgtt cgactcaagt
     2941 tttaccgaat tcttaccgat tgtctttatc aactcggggg tttcgtcatt tttttgacga
     3001 caacaccaga gtcaaaaaaa atatagcgtt ctttaggatt cgctctgcta caatcttcgg
     3061 tggccttgca atcggatctt acggaagggg tttggtacat ggaccagaaa agcttaacga
     3121 aatttaaaaa aatgctgctc gaagaacgcc aaaacgttct caatcactcg aagaaagatc
     3181 gaaatgacat aattgtcgat tccgacgatt tagcagacga aacagattta gcggcaagtg
     3241 aggtcagcca agcgcttgca tttagcctac gcgaccgcga acgcctattg ctttctaaga
     3301 tcgaccaggc gcttgaaaaa atcgaagagg gtactttcgg catctgcgaa acttgtgaag
     3361 aacccataga gattcgccgc ctcgaagcga gacctgtaag caatctttgc ctatcgtgca
     3421 aagaactcga agagcataaa gaaaagatct tcgcctaacc gttaccgagc gattatccaa
     3481 ccctaacgac ctgttggcca aacaaggctc gggctcgtct tttttacccc tgaaggcttc
     3541 ccgggcaata aacgtcctaa ccccgccccg ttcaagctgt tttcgaaaat agaatcctaa
     3601 agctttggcc cgcctacacc gaatcgattc tcaagaggct agtcaatagg catcaaggaa
     3661 gattgccaac gctgaggaga ctaagttcaa tggtaacaaa ctacgacggc gaacaaatcg
     3721 aagttcctgc aactctcccg atgctccctg taagggatat cgtggtgttc ccctacatga
     3781 tcattccgct ctttgtggga cgcgaatctt cgatcaaagc agtagaagag agtttggctc
     3841 gcaatgaccg tttaatactg ctagccagtc aaaaagatat ttcggacgaa catccaaatc
     3901 ccgacggaat ttaccaaatt ggcaccgtgg caatgatcat gcgcatgcgc aagttgccgg
     3961 acggccgcat taaaattctc acccaaggtc tttgtaaggc tcatatagac gagt
//
LOCUS       DAOMXO010000141         4005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12216_length_4005_cov_5.129873, whole genome shotgun sequence.
ACCESSION   DAOMXO010000141 DAOMXO010000000
VERSION     DAOMXO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12216_length_4005_cov_5.129873"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1082)
                     /locus_tag="PLH57_04045"
     CDS             complement(99..1082)
                     /locus_tag="PLH57_04045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRK01811.1"
                     /translation="MIKRVQSGASCVIVFSILATILAQGLFLRSAHSALIDRIVATVN
                     NEVILQSDFDKFQRTFKLRREIDPILGFDKSMGDSPSRAKIQEFLIQERLITQTFRAT
                     EADIDQEIQAVMRNANVSRREELDQFLRREGFSPKEYEELIAVAVSKRALIDREIRSR
                     VNISDDDVRNDFYNKALKNSSIPLEYKIQLIIVDVAQHDSPQVAQNIAERALRAINQG
                     EAFEDVIRYSDDPSKENLGIIDDYLSSDQLVEPIRSAVKGLQIGSHSKVLKNDRSYII
                     VKLLDARSMESKQFVEAKERIRQELAEVEYEKQLYLWADRARNDAYVKVNE"
     gene            complement(1146..2042)
                     /locus_tag="PLH57_04050"
     CDS             complement(1146..2042)
                     /locus_tag="PLH57_04050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRK01812.1"
                     /translation="MQQSRSLISNLFPLLAVPVALGIAVTVHGATELARVNSKVITLE
                     EFNTKYRDSMRFFRAKTPTKKNVLDEIINRDLGIAEAKKIGLDKDPEVVERINTVLFH
                     AFVDKQLASKFDSIKISEKDAREYYKKNPEIRTSHIFVSVRFDANPAQEREALTKITR
                     IKKALDEALKSGKATFAEVAVAESEGVAAAAGGDIDYQTKDKLDPIYYQSAVALGKVG
                     NVSGIVRSQYGYHIIKLTGKKEYTEVDPGVVRRLVFEEKRSEIYDDYMASLRARSQVS
                     VNYNLLSEKKTAEAPRADTKKN"
     gene            complement(2113..>4005)
                     /gene="mfd"
                     /locus_tag="PLH57_04055"
     CDS             complement(2113..>4005)
                     /gene="mfd"
                     /locus_tag="PLH57_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRK01813.1"
                     /translation="GDKLYIPIYRLEQVQRYIGDSDGSLIPLDKLGSAQFEKTKSKVR
                     EALKEVAHELLRIYAMRATQSGFKFSPPDEQFKEFEARFPFAETPDQLRAIDETLTDM
                     QSGKIMDRLICGDVGYGKTEVAMRAAYKAALDGKQVAVLVPTTVLALQHELSFKNRFQ
                     DTGVRVASVSRFKSSKELKETLTEVKEGKVDILIGTHRILSRDVEFKDLGMVVVDEEQ
                     RFGVEHKEKLKKLKVTTAILTLTATPIPRTLHLSLMGLRDISVINTAPVDRLSVRTYI
                     SHFEEEALRIAITQEIARGGQVFFIHNRVQSIGAMEERLKKIVPQARIVVAHGQLKEG
                     QLEEKMVEFYSKKADVLLCTTIIESGLDIPSANTIIINRADMFGLAQLYQLRGRVGRS
                     QVRAYCYLLLPEEGLVTDDAKKRLEIIQRFVDLGSGFKVASHDLELRGGGDILGKAQS
                     GHIAAVGYELYTDLLEETIHEIKGEEIEDRFDPEIKIPVPALLPDDYVPDIQQRLGLY
                     KRLSQADSIDKISDLEAELIERFGQPPQSVLNLLELIQIKQLLVHHHIKTLLVGQDRI
                     SLDASEARALAPEKILSIVKKSPSTVSITPESKILLRKPFTDAKTLHADLRQFLSTVS
                     SDAASS"
BASE COUNT          972 a          929 c          921 g         1183 t
ORIGIN      
        1 aaaaatcctt tccaagacgt gacaaccctt ttgcgataac ctctggaccg attccaaagt
       61 ggtcgccagt cgtaattgca aaataggggc ttagtggatt attcattcac cttaacgtaa
      121 gcgtcatttc gtgctcggtc cgcccagaga tatagctgtt tttcatactc gacttccgcg
      181 agttcctggc gaattctctc ttttgcttca acgaactgtt ttgactccat agagcgggca
      241 tccaacaact ttactataat gtagctgcgg tcatttttta gaaccttact atggctacca
      301 atctgtaacc ccttcaccgc cgaacgaatt ggttctacaa gctgatcgga tgaaaggtag
      361 tcatcgataa ttcctaaatt ctctttcgat ggatcgtcgc tatatctaat gacgtcttcg
      421 aatgcctcac cctggttaat ggcacgaagt gcacgttctg ctatattttg cgcaacttgc
      481 ggcgaatcat gttgtgccac gtctacgatg ataagttgaa ttttatattc aagcggaatt
      541 gaggaatttt tgagggcctt attgtagaaa tcattacgaa catcgtcgtc acttatattt
      601 acgcggctac gaatttcccg gtcaattaag gcgcgctttg atacggctac cgcaattagt
      661 tcttcgtatt ccttcggaga aaatccttca cgcctcaaaa attgatctaa ctcttcgcgc
      721 cgggaaacat ttgcattcct cattacggct tgaatttcct ggtcgatgtc ggcctctgtg
      781 gcgcgaaaag tttgagtgat taatcgttcc tgaataagaa attcttgaat tttagcccga
      841 gaaggagagt cgcccataga tttgtcgaat cccaaaatcg gatcaatctc tcgacgaagc
      901 ttgaaggttc tttggaattt atcgaaatcg gactgaagaa ttacctcatt attgaccgtt
      961 gccacaatgc ggtcgataag cgcactgtgg gctgacctca gaaacagacc ttgagctaaa
     1021 atggtcgcca atatggaaaa gacaatcaca caggaagcac ctgattgcac tcgtttgatc
     1081 atgcctttac tgctaccgca acccgcaggt tttagcaaaa aatcgcggct gaagtggcgg
     1141 aggatttagt tcttcttagt gtctgctctt ggcgcttcag cggtcttttt ctctgaaaga
     1201 aggttgtaat tgaccgaaac ctggctccga gcccgaagcg aggccatgta atcgtcgtag
     1261 atttcactgc gtttttcttc aaataccagc ctgcgcacga cgcctgggtc aacttcggta
     1321 tattcctttt ttccggtcaa ctttatgatg tggtaaccat attgactgcg aacaatacca
     1381 ctgacgttgc ctacttttcc gagggccacc gccgactgat aataaattgg gtcgagcttg
     1441 tccttagttt gatagtcgat gtcaccgcca gccgctgccg caacgccttc ggactctgcc
     1501 accgctactt ccgcaaaggt tgcttttccg ctcttgagtg cttcgtcgag agctttttta
     1561 atgcgcgtga ttttagttag cgcttcccgc tcctgagcgg ggttggcatc gaatctaacg
     1621 gagacaaaaa tatgggatgt tcgaatttca ggattctttt tataatactc gcgagcatcc
     1681 ttttcggaaa ttttgatcga atcgaatttg ctggcaagtt gtttgtcgac aaaggcgtga
     1741 aataaaacag tattaattcg ttccaccact tcgggatctt tatcgaggcc aatcttcttt
     1801 gcttcagcga ttcccaagtc gcgattaatt atttcgtcga gcacgttctt tttggtgggg
     1861 gtttttgccc ggaaaaaacg catactatcg cggtatttcg tattgaactc ttcaagagtg
     1921 attactttag agtttacacg cgctagttcc gtagccccgt gaaccgttac cgcaatgcca
     1981 agtgcgacgg gtacggccaa aagtgggaaa agattagaaa ttagactacg cgattgttgc
     2041 atggtgatgc cccctagtag caccatttta gccacgtcct gtgtttaacg caaacaatca
     2101 ggtatataga agttaactcg atgcagcatc gctcgagact gtactcaaga attgacgcag
     2161 atcggcgtga agtgttttgg cgtctgtaaa cggctttcta aggagtattt ttgattccgg
     2221 ggtgatcgag acagtcgagg ggctcttttt tacgatactt agaatcttct ctggcgctag
     2281 cgcgcgcgct tcagaagcat caagcgagat gcgatcctgg cctaccagca aagtctttat
     2341 gtgatgatga acgagcaatt gcttaatttg gatcagctca agcaagttca gaacgctttg
     2401 gggaggctgg ccaaaacgtt ctattagttc ggcctcgagg tcagaaattt tgtcgatgga
     2461 atcggcttgg gacaatcttt tgtacagacc caaccgctgt tgaatgtcgg gaacataatc
     2521 gtcaggaaga agtgccggca ctggaatttt aatttctgga tcgaagcgat cttcaatctc
     2581 ttcaccttta atttcgtgaa tcgtttcttc taatagatcg gtgtagagtt cgtatccgac
     2641 tgccgcaata tgtccagact gtgctttgcc taaaatgtcg ccgccccctc gcagctctag
     2701 gtcgtgggaa gccactttaa agcccgaccc taaatcgaca aaccgttgaa taatctcgag
     2761 tcgctttttt gcgtcatctg taacaaggcc ctcttcggga agtagcaggt agcaataggc
     2821 tcgcacttgc gacctcccta ctcgaccgcg tagctgatag agttgggcga gtccaaacat
     2881 gtctgcgcgg ttgataatga tcgtgtttgc tgaaggaatg tctaacccag actcgattat
     2941 ggtggtgcaa agcagtacat cagctttttt actgtagaac tcgaccattt tttcttcgag
     3001 ctgaccttct ttaagctgcc catgagccac gactatacgg gcttgtggaa cgattttttt
     3061 taagcgctct tccatcgctc caatgctttg aactcggttg tgaataaaga agacttgtcc
     3121 gccgcgggcg atttcctggg ttatggcaat tcgcaacgcc tcttcttcaa agtgcgaaat
     3181 ataggttcta acgctgagtc gatcgaccgg cgcggtgttg atgactgata tgtcgcgcaa
     3241 tcccatgagc gacaaatgca atgttcgggg tattggggtg gcagttaatg ttagaatagc
     3301 agttgtaact ttgagtttct tcaacttttc tttgtgttca actccaaatc gttgttcctc
     3361 gtcgacgacc accataccta gatctttaaa ttctacgtca cgtgagagaa ttcgatgggt
     3421 tccgataagg atgtcgacct tcccttcttt gacctccgtt agagtttctt ttaattcttt
     3481 ggatgacttg aacctagata cagaagcgac tcgaaccccg gtatcttgaa aacgattttt
     3541 aaaagaaagt tcgtgttgaa gagctaaaac ggtagtcggt acgagtacgg cgacctgctt
     3601 tccgtccaaa gctgccttgt acgcggcccg catcgcgact tcagttttgc catatcctac
     3661 gtcgccacaa atcaggcggt ccattatttt accgctttgc atgtcagtta aggtttcatc
     3721 aatggctcgt agttggtccg gtgtttccgc aaatggaaat cgggcctcga attccttaaa
     3781 ctgctcgtca ggcggagaaa atttaaaacc cgattgagta gcccgcatcg cgtaaatacg
     3841 cagaagttcg tgagctacct ctttaagtgc ttccctgact ttgctttttg ttttttcaaa
     3901 ttgagcactg ccgagtttgt cgagcggtat aagcgaacca tcggaatcgc cgatgtatct
     3961 ttgaacctgt tctaggcggt aaatgggaat atatagttta tcgcc
//
LOCUS       DAOMXO010000142         4001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12236_length_4001_cov_4.405727, whole genome shotgun sequence.
ACCESSION   DAOMXO010000142 DAOMXO010000000
VERSION     DAOMXO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12236_length_4001_cov_4.405727"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLH57_04060"
     CDS             complement(<1..320)
                     /locus_tag="PLH57_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01814.1"
                     /translation="MFLNTTPSLAHIGHDHGSAPEIHDREELKQLDSNLALRLEQIVQ
                     ESDLRSYEQLVSEVEKIAHELQDLEPGRSKTGESGKHFHRTKKLFSTAGKILKSFASR
                     LVGI"
     gene            complement(406..1491)
                     /locus_tag="PLH57_04065"
     CDS             complement(406..1491)
                     /locus_tag="PLH57_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRK01815.1"
                     /translation="MPKIARSRTLKKAIPRNTGTLWLPAFCDLHIHGAFGLDFMAINA
                     VSEKTFLSELRRVSELLCQHGIAHYAPTLLTSEWSTLEATVAKWAKLLSSLRSQPRIW
                     PKNAALPIGLHLEGPFLQPEYAGAHPRSAILAPTAPRLRRLLSCGSGHIRIVTLAPEI
                     TGAIPAIKTLVKAGVRVQLGHSRANTAQSLAAINAGARGLTHLFNAMRTHHRDPGLEA
                     ALCWPNVTAEIITDGVHVHPAKVYQLAKAGSDRHYAVSDCCAVAHAPKASLQTLGELR
                     VTQHGGAAYVANKHGRATTTLAGSARLLPEHPALLYRALTQDLSLPIDKKTLLSLFTP
                     PAFAGVKTRFFANFEAETLRFVRCAER"
     gene            1599..2333
                     /locus_tag="PLH57_04070"
     CDS             1599..2333
                     /locus_tag="PLH57_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01816.1"
                     /translation="MPAKSGASRPLKTKAKPNMAKAAKSKPVAKTASAPKGKVLKTLG
                     SAVSKLVGRGKSHAQPSKKGAATKKAQETPAKAEKAAKTASKETSVPAKQAGKAASVG
                     KGKDFKKSVGQARTALEKVSGPHRAISVSSAICREVACELMSTTGGYCRMHYIKNWKK
                     IKRKELILKEKKLNHYIEELVSKYPDKYIEAIRVDLASEKDFAKVIADLEIDEALEDF
                     EIETPENTDEGLLDNLKRFDDDGDVF"
     gene            complement(2357..2854)
                     /locus_tag="PLH57_04075"
     CDS             complement(2357..2854)
                     /locus_tag="PLH57_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01817.1"
                     /translation="MDRRKDTIFKGTSLPKEYLEMVQALFNKNFKKHLSAREVFLAFG
                     EVFPDELVMAVSMKHSGQLGMTTCYGSVDFPLANGPKGTANQVQAAAGQCIDAIASFF
                     GTFFDEKRPVDYDNEYRQMWTAVELEKNKKTYLRINRDNLELDAQADAILELDIEKRN
                     KKKLN"
     gene            complement(2857..>4001)
                     /locus_tag="PLH57_04080"
     CDS             complement(2857..>4001)
                     /locus_tag="PLH57_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRK01818.1"
                     /translation="TYIGWKKESTLDITSLPMKTKLRLSAHVLLIYSLLFSWPTAAHS
                     APPGLANQLPDLVENVIDGVVNISSTRFVKQWVLRGMPEFFGMFGIPEEHVSKQSSLG
                     SGFLIDGDGYIITNNHVIEGADEVVITFRDNRRVPAKIVGRDKLMDLALLQLRGGKNN
                     KNLKPVKLGNSDSVRIAEYVFAIGNPFGLGHTVTDGIISQKNRSIGIGVLDNFLQTNA
                     SINFGNSGGPLFNFKGEVIGINTAINGQGQLLGFAIPIDEARSKLEDLKKYGYIIRAW
                     LGILGINITPAIQYNYDLPVDHGVAITDLVRSGPAADVGLKPGDIIVTLADKEIAARG
                     DIQKILSKFKPGEKVAIGIRRGGKKLTYTVKLEVSPDTSKLPQEVL"
BASE COUNT         1017 a          937 c         1069 g          978 t
ORIGIN      
        1 aatatcccaa ccaagcgact tgcgaaggac ttaagaattt ttccagcagt tgaaaataat
       61 ttctttgttc tatgaaagtg tttgcccgat tcgccggtct tcgagcgacc cggttccaga
      121 tcttgcagct cgtgggcgat tttttcgact tcgctcacca attgttcgta ggagcgaaga
      181 tccgattcct ggacaatttg ctccagacgc aaggccaagt tcgagtctaa ttgtttgagt
      241 tcttcgcgat catgaatttc aggcgcggaa ccgtgatcgt gtccaatatg tgcgagtgaa
      301 ggggtcgtat ttaaaaacac cccagcgcca agaagagcaa aaataagaga cggccaaaat
      361 attctgaaaa tagctcacca aatcaagaat tagttgggcg aaggattaac gttccgcaca
      421 acgaacaaat cggagtgttt ctgcctcaaa gttcgcaaaa aagcgagttt ttacgcccgc
      481 aaaggcggga ggggtgaata gcgatagcag agtttttttg tcgataggaa gactgaggtc
      541 ttgcgtcaag gcccggtaga gtagggccgg atgctccggc aggagacgag ccgacccagc
      601 taacgtcgtc gtggcgcgcc cgtgtttgtt ggccacgtaa gcagctccac cgtgctgcgt
      661 gacccgcaat tcgcccaagg tctgaagaga ggctttgggg gcgtgggcta ccgcgcaaca
      721 atcgctcacg gcgtaatgtc ggtctgagcc ggccttggcc aactggtaaa ccttcgcggg
      781 gtgcacgtgt acgccatcgg tgatgatttc ggccgtaacg ttcggccaac agagcgccgc
      841 ttcgagaccg ggatcgcgat ggtgagtccg catcgcgttg aagaggtggg tgagtccccg
      901 ggccccggcg ttgatggcag ctagtgactg cgctgtgttc gcgcggctgt gcccgagttg
      961 gactcgaacg ccggccttaa cgagggtctt tatggcaggg atagcgcctg taatttcggg
     1021 cgcgagcgtg acgatgcgta tgtgccccga accacacgat agtaggcgcc ggaggcgcgg
     1081 cgcagtgggg gctaatatcg ccgagcgagg atgagcgccg gcgtattcgg gctgaagaaa
     1141 tggcccttct aaatgaagcc cgatggggag agctgcgttt tttggccaga ttcgtggctg
     1201 acttcgcaag ctcgacaaaa gtttagccca ttttgccacg gtcgcttcta gggtagacca
     1261 ctcgctggta agaagagtgg gcgcgtagtg ggcgatgccg tgttggcata acaactcact
     1321 cacgcgtcgg agttcactta aaaatgtttt ttccgaaacc gcattgatgg ccatgaaatc
     1381 taacccaaaa gcgccgtgaa tatgaagatc acaaaatgcg ggaagccaca gggttccggt
     1441 gtttcgggga atggcctttt ttagggttcg ggaacgggct atttttggca ttggggcccc
     1501 ttgggttgga tcatacttga ctttattggc ttgactttgt tgcgccgcaa acttattccg
     1561 ttctcaatag agaaaaattt acggagatta ctatctttat gccagcgaaa agtggcgcct
     1621 cgcgccctct gaaaacaaaa gccaaaccga acatggccaa ggccgctaaa agcaaacccg
     1681 ttgcaaagac ggccagtgct ccaaaaggca aggttctcaa gacgttggga agcgccgttt
     1741 cgaagcttgt tgggcgaggt aagtcacacg cccaaccctc caaaaaggga gccgctacaa
     1801 agaaagcgca ggagacccct gcaaaggctg agaaagccgc aaagactgcg agcaaggaaa
     1861 cttcagttcc tgcaaagcaa gcaggtaaag cggcttcagt tgggaaaggc aaagatttta
     1921 agaaatccgt gggccaagcg cgcactgcgc ttgagaaagt gtccggccca caccgtgcaa
     1981 tttcagtgag tagcgccatt tgtcgcgaag tcgcctgtga gttgatgtca actacaggtg
     2041 gttattgccg catgcactac attaaaaact ggaagaaaat taaacgcaaa gagctcattc
     2101 tcaaagaaaa gaagctcaat cactacatcg aagaattggt ttcgaaatat ccagataagt
     2161 atattgaggc gattcgtgta gaccttgcca gtgaaaaaga ttttgccaaa gtcatagccg
     2221 atcttgaaat tgacgaagct ctcgaagatt tcgaaatcga aacgcccgag aacaccgatg
     2281 agggacttct cgataatctc aagcgttttg acgatgacgg cgatgttttt taacgaagat
     2341 atcgcgcgct aaatttttaa tttagcttct tcttatttct tttttctatg tcgagttcga
     2401 gtatcgcgtc ggcctgggcg tcaagctcta ggttgtcgcg gttgattcga agataagttt
     2461 tcttattttt ttctagctca acggcagtcc acatctggcg gtactcattg tcgtagtcga
     2521 ccggacgttt ttcatcgaaa aaagttccaa aaaaactggc gatcgcgtcg atgcactgac
     2581 cagcggcggc ctggacctga ttcgcagtgc ctttcggacc atttgcgagc ggaaaatcca
     2641 ccgagccgta acaagtggtc ataccaagct ggccggagtg tttcattgaa acggccatga
     2701 ctagttcgtc gggaaatact tcaccaaacg ctaagaaaac ttctctagcg gaaagatgtt
     2761 tcttaaaatt tttattgaat agcgcctgaa ccatttccaa gtattcttta ggcaatgatg
     2821 tgcctttaaa aatagtgtcc ttacgacgat ccatgactat aaaacctcct gcggtagttt
     2881 ggacgtatcg ggtgaaactt caagtttgac agtgtaggtg agtttcttgc caccacggcg
     2941 aataccaatt gccacttttt cacccggttt aaattttgaa agtattttct gaatatctcc
     3001 gcgagccgcg atttccttgt cggcgagggt cacaatgatg tcaccgggtt ttaaacccac
     3061 atcggcggcc ggacctgaac ggacgagatc agtgatcgcc actccatggt ctaccggcaa
     3121 atcataattg tattgaatgg ccggcgtaat attgatgccg agtattccga gccaggcgcg
     3181 aatgatatag ccgtattttt taagatcttc gagtttgctt cgcgcttcgt cgatagggat
     3241 ggcaaaaccc agcaattggc cctggccatt gatcgcggtg ttgattccga tcacttctcc
     3301 cttgaaatta aacaatgggc ccccggagtt cccgaaatta atactagcgt tggtttgcaa
     3361 aaaattatcg agtacgccaa taccgattga gcgatttttc tgactgataa tgccgtcggt
     3421 taccgtgtgg ccaagcccaa aagggtttcc gatcgcgaaa acgtattcgg caattcgcac
     3481 agaatcacta ttgccgagtt ttaccggttt taggttttta ttgttcttgc cgccccgcaa
     3541 ctgaagcagc gctagatcca tgagtttgtc gcgaccgacg attttcgcgg gcacgcggcg
     3601 attgtcgcga aaagtgatga cgacttcgtc ggccccttcg atgacgtggt tgttggtgat
     3661 gatgtagccg tcgccatcaa tgagaaatcc ggagccgagt gacgactgct ttgagacatg
     3721 ttcctcggga atgccgaaca ttccgaaaaa ctcgggcata ccgcgtagca cccactgttt
     3781 aacgaatcgc gtcgaggaaa tattaacaac accatcgatc acgttttcga cgagatcggg
     3841 caattggttg gcaaggcccg gaggtgcact atgagccgcc gtgggccaag aaaacaggag
     3901 cgagtaaata agaaggacat gggcggacaa acgaagttta gttttcatag gaagactggt
     3961 tatatcgaga gtagactctt ttttccagcc tatgtatgtt g
//
LOCUS       DAOMXO010000143         3987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12307_length_3987_cov_4.596134, whole genome shotgun sequence.
ACCESSION   DAOMXO010000143 DAOMXO010000000
VERSION     DAOMXO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12307_length_3987_cov_4.596134"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLH57_04085"
     CDS             <1..199
                     /locus_tag="PLH57_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01819.1"
                     /translation="GYFEVIQFLRGEMTLPECRERVVISTRQLAKKQMTFFKTFPQSI
                     DWFELPSAEEAFLNAARIVLG"
     gene            212..1060
                     /locus_tag="PLH57_04090"
     CDS             212..1060
                     /locus_tag="PLH57_04090"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRK01820.1"
                     /translation="MTETNSVKELRLVILSDGTGETAAQITKAAMLQFSDKEIYFTRY
                     KNIRTKAQVESIFEEAAIHHDLIVYTVVSPELREYIANKALEKKVPVIDILGPLLNSM
                     AAFFNLAPKALPGLFHQVDDRYFKRIEAIEYTLAHDDGRDLTNLDNADVIILGISRTS
                     KTPLSMYLGHQGWKVVNIPIIKGLEIPKEIFEADQKKIVCLTISPDTLATIRKNRLER
                     LGQEEGGDYASIDRVMDEIEYADQIFRKNKRWPVFDVTAKALEETAAEIIKLMLNRKK
                     QAANPP"
     gene            1082..2017
                     /locus_tag="PLH57_04095"
     CDS             1082..2017
                     /locus_tag="PLH57_04095"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HRK01821.1"
                     /translation="MSSKSGRQLGKAVWWNGKWTNSEDAKISLFTHSLHYGLGAFEGI
                     RCYKTKTGQAIFRLPEHTRRFFDSCKILEIKIPYTEKQINQAITETVRRSGLKSGCYI
                     RPLVFLGDGPLGVFPGFDPPVEVAIMTWVWGAYLGAEAKAKGARIKISSFSRGAVNHT
                     MSKAKLTGSYSTGILAKLEVKRMGYDEALLLDTDGFVAEGSGENIFIYRDGLLKTTPL
                     TSVLPGITRATIIQLAQAEGIPVQEARFTRDELYTAEEAFFTGTAAEVTPIREVDDRK
                     IGSGKPGEITKSLSKKFFDIVEGRNPKYSKWLTKI"
     gene            2020..2316
                     /locus_tag="PLH57_04100"
     CDS             2020..2316
                     /locus_tag="PLH57_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 family protein"
                     /protein_id="HRK01822.1"
                     /translation="MYVGVAKVILDFHGNVEIKTKRFHMRALLEELRKKFEVSAVELA
                     DFDDCERCVLGFSVTQANEKSAKAALAKILEHIDQTAFARVTVEDSDVLPFDIA"
     gene            complement(2326..3345)
                     /gene="rfaE1"
                     /locus_tag="PLH57_04105"
     CDS             complement(2326..3345)
                     /gene="rfaE1"
                     /locus_tag="PLH57_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HRK01823.1"
                     /translation="MQKTHQLKSKRVDQITRKFDRKKIVVFGDVGVDKYTVGKVSRIS
                     PEAPIPIVEVFDTRLKLGLAANVADNIVEMGGVPLLVGVVGQDSGADDFFSLLRKRKI
                     PKNHVVQDATRKTTLKERVVAEAQQVVRIDHETQSRIEAKLIEKIGKALERAINEAAA
                     LVVEDYAKGLMETSLVRRAIETAAAKGIPILVDPNVRSNYAVYAGCTVITPNILEAEA
                     LSKIRIVDASSLKQAGARLQQEIGAEIVIITRGKDGMAIFKRGRPNPILIPTFAREVF
                     DVSGAGDTVIATLALSLASGANIEEAALLANFAAGVEVSKRGTATVSVKEMKEHMRYV
                     GALRR"
     gene            complement(3308..>3987)
                     /locus_tag="PLH57_04110"
     CDS             complement(3308..>3987)
                     /locus_tag="PLH57_04110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRK01824.1"
                     /translation="TELVRHAIRVLGRDPLEKKEFQSKIVADPSLENGVIRDLSKLGL
                     EPGRFVLLHPGMGGSALNWSIDSYAELIRLIISRGRKVLVSGSSVDEDTVQKIVMRTR
                     EIGVEQAHLNVLLTYDLPHYIALLKLAGPIVAPSTGPLHLAAGLGKPCVSFYPPIQVQ
                     SSKRWGPRSRAAQKIFTPEVACGQKYNCIGSRCEFYYCMDRISPETVAKEVEKLAENA
                     SVEIQTC"
BASE COUNT         1143 a          947 c          878 g         1019 t
ORIGIN      
        1 cggatatttc gaggtcattc aatttttgcg gggcgaaatg acgcttcctg aatgtcgaga
       61 acgagttgtc atatccaccc ggcaacttgc aaaaaaacaa atgactttct ttaaaacctt
      121 tccgcagtca atcgactggt tcgaattacc ttcggcggag gaagcatttt taaatgcggc
      181 gcgcattgtg ctaggctaaa actcaacaac tatgaccgaa accaacagtg tcaaagaact
      241 acgacttgtc attctctcag atggtacagg agaaactgcg gcgcaaatta ccaaagcggc
      301 tatgttgcag ttttccgaca aagaaattta ctttacgcgc tataaaaaca ttcgaaccaa
      361 agcacaggtt gaatcaattt ttgaagaagc cgccattcac catgatttga tcgtttacac
      421 ggtcgtttcg ccggaacttc gagaatatat tgcaaacaag gcgcttgaaa aaaaggtgcc
      481 ggtaatcgac attttgggcc ctcttcttaa ttcaatggcg gcgtttttta atctcgcacc
      541 taaggcccta ccgggactgt tccatcaagt cgacgaccgc tactttaaac gcatagaagc
      601 aattgaatat acgctcgcgc atgatgacgg aagggatctt actaaccttg ataacgcaga
      661 cgtcattata ttaggaattt cgcgcacctc gaaaactccg ctgtcaatgt acctcggcca
      721 ccagggttgg aaagtcgtga atatcccgat tattaaagga ttagaaattc ccaaagagat
      781 tttcgaagca gatcagaaaa aaattgtttg cttaacgatt agtcctgaca ctcttgcaac
      841 aatacgaaaa aatcgactcg aacggctcgg acaagaagag ggcggtgatt acgctagtat
      901 cgatcgagtg atggacgaaa tagaatacgc cgatcagata tttagaaaaa acaagcgatg
      961 gccagtgttt gacgttacgg cgaaagcact cgaagaaact gccgcggaaa ttattaagct
     1021 catgctcaac cgaaagaagc aagccgccaa tcctccatag aggcaactag aaaggaatct
     1081 catgagttcg aagagtggaa gacagttggg aaaagcagtc tggtggaacg gtaaatggac
     1141 taattcagag gacgcaaaaa ttagcctttt tacccattcc ttgcactacg gtttaggggc
     1201 attcgaaggc attcgttgtt acaagactaa gacgggtcag gccatatttc ggctgccaga
     1261 acacactcga cggttttttg attcttgtaa aattcttgaa ataaaaattc cctatactga
     1321 aaaacaaatt aaccaagcca taactgaaac cgtcagaaga tcgggactca aaagtggttg
     1381 ttacatacgc cccttggttt tcttaggaga cggcccacta ggcgtatttc cagggttcga
     1441 tccacccgtc gaagtcgcga ttatgacctg ggtttggggc gcgtatcttg gtgcggaagc
     1501 taaggcgaaa ggagctagaa ttaaaatttc tagtttcagt cgtggcgcag tgaaccacac
     1561 aatgagtaaa gcgaaattaa ctggtagtta ttcaactggt attctcgcca aactcgaagt
     1621 caaacgcatg ggctacgatg aagcgctcct tctagatacc gatggtttcg tcgctgaagg
     1681 gtcaggggaa aatattttta tctacagaga cgggcttctg aaaacaacac ccttgacttc
     1741 agttttgcca ggaatcaccc gcgccacgat tattcaactt gcccaggctg aaggcattcc
     1801 ggtacaggaa gcgcgtttta cgcgtgacga actatatact gcggaagaag ctttttttac
     1861 gggcactgca gccgaagtaa cgccaattcg agaggtagac gaccgtaaaa tcggatccgg
     1921 aaaacctgga gaaattacaa aatctttatc aaaaaaattc ttcgatatag tcgaagggcg
     1981 aaacccaaaa tacagcaagt ggttgactaa aatttaacta tgtatgtcgg agttgcaaaa
     2041 gtaattctcg attttcatgg aaacgtcgaa ataaaaacca agcgttttca tatgcgcgca
     2101 ctgttagagg agcttagaaa aaaattcgag gtttcagcgg tggaactcgc agacttcgat
     2161 gattgcgagc gctgtgtcct tggtttttcg gtcactcaag ccaatgaaaa atcggctaaa
     2221 gccgccctgg cgaaaatact tgaacacata gaccaaaccg ccttcgctcg agtaactgta
     2281 gaagactccg acgtattgcc tttcgacatt gcctgaagtc tcatcttaac gtcgaagtgc
     2341 tccaacataa cgcatgtgtt ctttcatttc cttaactgaa acagttgcag ttccacgttt
     2401 cgaaacttcg acaccggcgg caaaattagc aagcagtgcg gcttcttcaa tattagcacc
     2461 cgacgccagc gaaagagcga gcgttgcgat cacagtatca ccagcgcctg atacatcgaa
     2521 aacttctctg gcaaaagtag gtatgagtat cgggttcggt cgtcctcgtt taaagatagc
     2581 catgccatct ttaccacgag tgataatgac gatttcagcg ccaatttcct gctgtaaccg
     2641 tgctcccgct tgcttcaacg agctagcgtc gacgatgcga attttactaa gagcttcggc
     2701 ctccaaaata tttggagtga tcaccgtaca gcccgcatac actgcgtagt ttgagcgaac
     2761 gttaggatcg actaaaatcg gtattccctt ggcagccgca gtttcaattg cgcgacgaac
     2821 tagcgaggtt tccattaacc ccttcgcata atcttcaact actaaagctg ccgcttcatt
     2881 aattgctcgt tctaaggctt tccctatttt ttcgataagt ttagcttcaa ttctactttg
     2941 ggtttcgtga tcaattcgca caacctgctg agcttcagcc accacacgtt ctttcagcgt
     3001 tgtttttcga gtcgcgtctt gaaccacatg atttttagga attttacgct ttcgtagtaa
     3061 agaaaaaaaa tcgtcagccc cagagtcctg accgactacc cctactagga gcggaacccc
     3121 gcccatttcc acaatattat ctgcgacgtt ggcggcaagt ccgagtttaa gccgcgtatc
     3181 gaaaacttct acaattggaa tcggggcctc gggggaaatt cgagagacct tcccaacagt
     3241 gtatttatcg acacccacat caccaaatac gacgatcttt tttcggtcaa actttcgagt
     3301 gatctgatca acacgtttgg atttcaactg atgcgttttc tgcaagtttc tctacctctt
     3361 tggccactgt ctctggcgaa atacgatcca tacaataata aaactcacac cgtgaaccga
     3421 tacagttgta cttctgaccg caagcgactt ccggcgtaaa gattttttgg gctgctctgc
     3481 tcctagggcc ccagcgtttc gagctttgaa cttgaattgg aggatagaat gacacacacg
     3541 gttttccaag ccctgccgct aggtgaagtg gacctgtact cggtgccacg atgggtcccg
     3601 ctaacttaag cagcgcgata taatgcggca aatcataggt aagcagaacg tttaaatgtg
     3661 cttgttcgac cccaatctca cgagtacgca taacaatttt ttggacggta tcttcatcca
     3721 ccgacgatcc actaactagg accttgcggc cgcgcgaaat gatcaatcga atgagctcag
     3781 cataggaatc gatactccaa ttgagagccg accctcccat tccggggtgc aacaaaacaa
     3841 atcggccagg ctcaagtccc agcttcgaca aatctcgaat cacaccattt tctaaagaag
     3901 gatccgccac tatctttgat tggaattctt ttttttcaag cggatctcgg cccaacaccc
     3961 gaattgcgtg gcgcactagt tccgtat
//
LOCUS       DAOMXO010000144         3979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12336_length_3979_cov_4.509684, whole genome shotgun sequence.
ACCESSION   DAOMXO010000144 DAOMXO010000000
VERSION     DAOMXO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12336_length_3979_cov_4.509684"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLH57_04115"
     CDS             <1..876
                     /locus_tag="PLH57_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK01825.1"
                     /translation="GGHRVLGIEPTLMAKLATERGIPSIMEFFSKKTAEKAVAQAGRA
                     KVVTATNVFAHIENIHDIVSGIVSMLTDDGLFISESHYFNSLVETLQYDTIYHEHLRY
                     YSLKSLSYLFKMHGLEVIHAKKIPTHGGSIRVVAAPKGTRRPLGSVQQIMDEENKANL
                     GPESLLAFRDKVVQTKLDLYSLLSGIKQKGGKIYGVGAPSRASTLINYVGLDDGILDA
                     VLEIKGSYKIGKYMPGTVIPVLDENMLFEKQPEYALLLSWHIADELMPKLKARGYKGK
                     FIVPLPEPRVVSLDS"
     gene            876..1667
                     /locus_tag="PLH57_04120"
     CDS             876..1667
                     /locus_tag="PLH57_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK01826.1"
                     /translation="MTKLDLLIPVYNEGDNIHGVLAALNREVQTPFRMLICYDRDDDN
                     TLPAVKSFGPTKFSIEFVKNQGVGVHGAIMTGFQHGASESQAVIVMPADDDYNAGLID
                     KMFSEFERGADLVSASRFMRGGSMVGCHWVKATLVRISSFTLHFFARLPTRDATNGFR
                     LFSRKVLNALPFESTMGFSFSLECLVKCHRLRLKVTEVPAQWIERRVGKSRFQVLKWA
                     PAYLHWYFYAFQTTLLGKRAELPEALRTPPLTSNATPNRFQNPVV"
     gene            1717..2142
                     /locus_tag="PLH57_04125"
     CDS             1717..2142
                     /locus_tag="PLH57_04125"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRK01827.1"
                     /translation="MPADYVTVVALTQDLRVVLVEQYRPAAERVTLELPSGLVDPGEA
                     PEVTARRELFEETGFEVEELFSLGALIPDTGRLGNRLHCFFARVKESVRPRPKNNEGI
                     EVRIVTLETVKSAINDGTFDHALHCGVWMLAISKGFVPA"
     gene            2167..2670
                     /locus_tag="PLH57_04130"
     CDS             2167..2670
                     /locus_tag="PLH57_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HRK01828.1"
                     /translation="MQCYCGGTKPFSECCEPYLNGQALAPNPEALMRSRYTAHVKKNI
                     DYLRSTLVPKARTDFDAEASKEWAEKAEWLGLEILSARSNKVEFVAKYRIDGKTLEHH
                     EVSTFIKHGDRWFFVDGDSHVHEEGQGHDHHHEAQTPSVRQGPKVGRNDPCTCGSGKK
                     FKKCCGN"
     gene            complement(2667..3605)
                     /locus_tag="PLH57_04135"
     CDS             complement(2667..3605)
                     /locus_tag="PLH57_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01829.1"
                     /translation="MRILRQWRLLGPGVLFQLFVAGLLWTTSGICGPVVVHITFNDEK
                     AFTQGHAQFIKRIYEITLPEARYLEWSVDVPQIGRLSEYKYRNEIIDQMKRDLKLNDQ
                     ITNLVISTHGNTIKRDGHRFTRLDLIGEIGPNGLDPKALNFFAPLVGRFSKNAHIFLN
                     SCDTFRGPIAEVKVRAEAFMRALRIDSGKIYGAEIADDPFPLPNHRPVHSLMNYDRPN
                     IEFELNNSLVWYQGLVAMSAPSLGRRMYTASRMWFKKSIQKRTGFLISMKNGRADQVQ
                     GVHELRDADLVFGIPESSTNDCIKYLKEVAGVATDH"
     gene            complement(3659..>3979)
                     /locus_tag="PLH57_04140"
     CDS             complement(3659..>3979)
                     /locus_tag="PLH57_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01830.1"
                     /translation="KPKDRYLKLQEGTLGKVFSFQAGKTLMQGDQAMRVRVKVDTPES
                     LDKLYVAAGEAKLSDEAISPNIKKDLAAELLDPAKTEPESDRGLIRRFIGKCGDILRA
                     VLRK"
BASE COUNT         1030 a          986 c          931 g         1032 t
ORIGIN      
        1 ggggggcacc gcgttttggg aatagaaccg acactgatgg ccaaacttgc taccgaacgc
       61 ggtattccct cgatcatgga attttttagt aagaaaaccg ccgaaaaggc tgtggctcaa
      121 gctgggcgcg ctaaggttgt aactgccacg aacgtgttcg cgcatattga aaacattcac
      181 gatattgttt caggaattgt ttctatgctc accgacgacg ggctttttat atccgagtca
      241 cactatttca acagcctagt agaaacgctg cagtacgaca ctatttacca cgaacatctg
      301 cgatactatt cgctcaaaag cttgtcgtac ttatttaaaa tgcacggtct cgaagtcatt
      361 catgcaaaaa aaatcccgac tcacggcggt tcaatcagag ttgtcgcggc tccgaagggc
      421 acccgtcgcc cgctcggcag cgttcaacaa atcatggatg aagaaaataa ggccaacctc
      481 ggccctgaat cgctcttggc gtttcgcgat aaagtggttc aaactaaact cgatctctat
      541 tcgttgctct cgggcattaa acaaaaaggt ggaaaaattt atggcgtcgg tgctccatct
      601 cgcgcgagta ccttaatcaa ctacgttgga ctcgacgatg gcattttaga cgcagtgctt
      661 gagatcaagg gctcctacaa aattggcaaa tacatgcctg gaactgtgat cccggtactc
      721 gacgaaaata tgttgttcga aaaacaacca gagtacgccc tgctcctttc ttggcatatt
      781 gccgacgaac ttatgccaaa actgaaagct cgcggctaca agggcaaatt tatcgttccc
      841 cttcctgagc ctcgcgtggt gagtctggac tcctaatgac taaactcgat ttactcattc
      901 cggtttataa cgaaggtgac aatattcacg gtgtgctcgc ggcattaaac cgcgaagttc
      961 aaactccgtt tcgaatgttg atttgttatg accgggacga cgataacact ctacctgctg
     1021 taaagtcgtt tgggcctact aaattctcca ttgagttcgt gaaaaaccaa ggagttggtg
     1081 ttcacggggc aatcatgacc ggttttcagc atggagctag tgaatctcaa gcggtcatcg
     1141 tgatgccagc agacgacgac tacaacgctg gtttgatcga caaaatgttt tcagagttcg
     1201 aacgtggagc tgatttggtt tctgctagcc ggtttatgcg tggtggtagc atggtgggct
     1261 gccattgggt aaaagccacg cttgttcgta tttcttcgtt cacccttcac tttttcgcgc
     1321 gcctgcccac ccgcgacgca accaatggtt ttagattatt ttcgcgcaaa gtactcaacg
     1381 ctctaccatt tgaatccacg atgggttttt cgtttagctt agaatgtctc gtcaaatgcc
     1441 accgactgcg acttaaagtt accgaagttc cagcccagtg gattgaacgc cgcgtgggca
     1501 agagccggtt tcaggtatta aaatgggctc ccgcctattt acactggtac ttttacgcat
     1561 ttcaaacaac cttacttggt aagcgtgcag aattacccga ggcgcttcgg actcctcctt
     1621 taacatcaaa tgccacaccc aaccgttttc aaaaccccgt ggtttgaaat tcaaactcgc
     1681 gcttcgagaa ccggcgacgg accacattat gtggtgatgc ctgccgacta cgtaactgtg
     1741 gtcgcactaa cccaagacct acgggtagta ttggtcgaac aataccgccc ggccgccgaa
     1801 agagttacgc ttgaacttcc aagtggactc gtcgacccgg gagaagcacc ggaagtcact
     1861 gcacgacgag aactgtttga agaaacggga ttcgaggttg aggagctgtt ttcactcggt
     1921 gcgctcattc cggacactgg gcgtcttggc aatcggctgc attgtttttt tgcgagagtg
     1981 aaggaatccg tcagacctcg accaaaaaac aacgagggga tcgaagtacg aatcgttacg
     2041 ctcgaaacgg ttaaatcggc aattaatgat ggaacctttg accacgccct tcattgtggt
     2101 gtttggatgc tcgccatttc aaaaggcttt gttccagcct aaaagccgtg ttaacagtcg
     2161 ggctctatgc aatgctactg cggcggtact aaaccgtttt ctgaatgttg tgaaccttac
     2221 ttaaatggcc aggcgctagc gccaaacccc gaagcactta tgcgctcgcg ttatacagcg
     2281 catgttaaga aaaacatcga ttatctacga agcacgctcg tgcccaaagc gcgaacggat
     2341 ttcgatgcag aagcctctaa ggaatgggct gaaaaggctg agtggctcgg ccttgaaata
     2401 ctttcagctc gctcgaacaa ggtagagttt gtcgcgaagt atcgcatcga cggtaaaacc
     2461 ctcgaacatc acgaagtgtc gacgttcatt aaacacggcg atcgctggtt ttttgtcgac
     2521 ggtgactctc acgttcacga agaaggccaa gggcacgacc accatcacga agcacagact
     2581 ccctctgtcc gccaaggccc gaaagtcggg cgcaacgatc cttgcacttg cggaagcggc
     2641 aaaaaattca aaaaatgctg tggaaattag tggtcagtag cgaccccggc gacttctttt
     2701 aagtacttaa tgcaatcgtt ggttgatgac tcagggattc caaaaaccaa atcagcatct
     2761 ctaagttcgt ggactccctg cacttgatcg gctcggccgt ttttcatgga aataagaaat
     2821 ccggttctct tttgaatgct ttttttgaac cacattctac ttgccgtgta catacgtcgg
     2881 ccgagacttg gggcactcat cgcgacaagc ccttggtacc aaactaaact gttgtttagt
     2941 tcaaactcga tgttcggtcg atcatagttc ataagggaat gaactgggcg gtgattaggt
     3001 agaggaaacg gatcgtctgc gatttcggcg ccgtaaattt taccactatc tattctgaga
     3061 gcgcgcatga aggcctcagc tcgaaccttc acctccgcga tcggaccgcg aaacgtatcg
     3121 caggaattta agaaaatatg tgcgtttttg gaaaatcgtc caaccagcgg tgcaaaaaaa
     3181 ttcaatgctt ttgggtcaag gccattcggc ccaatctcgc caattaaatc aagacgagta
     3241 aaccgatgac catcacgttt aattgtattt ccgtgggttg agatcacgag attcgtaatt
     3301 tgatcattta acttcagatc gcgcttcatt tgatcaatga tctcatttcg atatttgtac
     3361 tccgacaatc gacctatctg aggaacatca acggaccatt ccaagtaccg ggcttcgggc
     3421 aacgtgattt catatatgcg cttaatgaat tgcgcatgcc cttgggtgaa cgctttttcg
     3481 tcgttaaagg tgatgtgcac taccaccggg ccgcagatac ccgacgttgt ccaaagtaaa
     3541 cccgccacga aaagctggaa tagaacgcct ggtcccaaca agcgccattg ccttaaaatt
     3601 ctcatgcggg gctcatacat gatcggggga ttttcttcaa actcccgact aatttagttt
     3661 attttcttag aacggctctg agaatatcgc cgcattttcc gataaaacgc cgaattaggc
     3721 cacggtcaga ttcgggctcg gttttggcag gatctagcag ttcagcagcg agatccttct
     3781 taatattagg tgaaatagct tcatcagata gttttgcctc gccagcagcc acatacagct
     3841 tgtcgaggct ttcgggggta tccactttta cacgcacccg catggcttga tccccttgca
     3901 ttagagtctt tccagcctga aaactaaaaa ccttccctaa ggttccttcc tgcaatttca
     3961 aataacgatc tttgggttt
//
LOCUS       DAOMXO010000145         3976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12349_length_3976_cov_5.073196, whole genome shotgun sequence.
ACCESSION   DAOMXO010000145 DAOMXO010000000
VERSION     DAOMXO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12349_length_3976_cov_5.073196"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /gene="radA"
                     /locus_tag="PLH57_04145"
     CDS             <1..1371
                     /gene="radA"
                     /locus_tag="PLH57_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRK01831.1"
                     /translation="TCQNCAAQAPKWMGRCSECGQWNTLVEETYKEPSQSAQTPSGSR
                     EALSQALPQNNRPLAIHEINIDEARRSTTRMGELDRVLGGGIVPGSLVLLGGDPGIGK
                     STILMQGLSATPEKPILYVSGEESANQIKLRGKRLGVDSKNFLVFAETRLEEIIEAAQ
                     KLRPGILIIDSIQTIFSTSVTSAPGSVSQVRECTGRLMLLAKTLEIATFVVGHVTKEG
                     SIAGPKVLEHMVDTVLYFESSDHSAYRILRAIKNRFGSTNEIGVFEMAEGGLREVQNP
                     SELFLQERTIDAPGTAIVSAVEGSRPILVELQTLVSQSTLAMPRRTTLGIDQNKVALL
                     VAVIEKRLGVPLYGHDIFAKVAGGIKLDEPATDLGIAAALLSSFKNRALDAKLLICGE
                     VGLTGEVRRISQLDLRLSEAAKLGFQRAIVPQSGLDALAKANFRAKIEVTGVRSLDEA
                     VKALDL"
     gene            complement(1372..2865)
                     /gene="glpK"
                     /locus_tag="PLH57_04150"
     CDS             complement(1372..2865)
                     /gene="glpK"
                     /locus_tag="PLH57_04150"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRK01832.1"
                     /translation="MELILAIDQGTTGSTVSIVDTDLNIHAQHDVDFKQIFPKPGWVE
                     HNPDHIWQSVTLAMRGALAKLKRKSPNIVGIGITNQRETSCFWNRKTGEPLSNAIVWQ
                     DRRTTDLCDELRKKGLEPQISKSTGLLIDPYFSASKILWSLKHNPNVSRAAKRGELAF
                     GTIDSYLVHRLTGGRVHITEPSNASRTMLFDLRSNEWNRDLCRIFQISVDSLPLVVNS
                     FSESFGKTYGVKELPNGIPITGVLGDQQSALLGQACVKSGQAKCTFGTGSFILMNTGP
                     RIVRSKHRLLTTVAWRWNDVSTYALEGGAFTAGAAVQWVRDGLELIPNSPAIEALART
                     VDTTDGVIFVPAFTGIGAPYWDPEARAGMLGCTRGTTKAHVARAVLEGIAHMNVEILE
                     AMKKDSKLPLRSLNVDGGASQNTLLMQMQADALNCSLVRPKIIETTSLGAVFAAGLGA
                     GVWRDFSAIEKAWKKDCVFQPRIKSLDRKKSELRWKRAVKAVRLFGS"
     gene            2885..3691
                     /locus_tag="PLH57_04155"
     CDS             2885..3691
                     /locus_tag="PLH57_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRK01833.1"
                     /translation="MRGFFGKRKEKLFDLHNLDFDKLAFYVLPRFLLEIVRKYLRLEI
                     EGAENLPARGKAIILPNHSGWAGFDAVMIGNEIFKSKKRIPRIMAHKGFFINTELKII
                     ARKMGMEEASTDNGVRLLKKNNIVMLFPEGEYGNFKPTAERYQLREFKRGFVKMALQA
                     NAPIIPTLVIGAEETHINLASLKLTKYLKGQIIPIPLNILPLPAKWKIKFLEPITLEK
                     YADVDPNDGEKLLQIAQEVRVQMQAQLDAELKKRKYVYFPTEDEGSNKTV"
BASE COUNT         1049 a          967 c          945 g         1015 t
ORIGIN      
        1 acctgccaaa actgcgcagc acaggcacca aaatggatgg gccgttgttc ggagtgcgga
       61 caatggaaca cacttgtaga agaaacctac aaagagcctt cccaatcagc tcaaacacct
      121 tcgggttctc gtgaagctct ttcacaggcc ttgccccaaa ataatcgccc gcttgccatt
      181 cacgaaatca atatcgacga ggccaggcgc tcaaccactc gaatggggga actagaccgg
      241 gttttaggtg ggggtatagt accgggaagt ttagtgcttt taggcggcga tcccggaatc
      301 ggtaagtcta ccattttaat gcaaggactt agcgccaccc ccgagaaacc aattctttat
      361 gtttcgggcg aggaaagcgc caaccaaatt aaacttcgcg gaaaaaggct tggtgtagat
      421 tcaaagaatt ttttggtatt cgctgagact cgtcttgaag aaattattga agctgctcag
      481 aaacttcgcc ctggaatttt gattatcgac agtattcaga caattttttc tacttccgta
      541 acttccgccc ctggcagcgt ttctcaggta cgtgaatgta ctggtcgtct catgcttttg
      601 gcgaaaactc tcgaaatagc aacttttgtg gtcggtcacg ttacaaagga aggttcaatt
      661 gctggtccga aagtacttga acatatggtc gacaccgtat tgtactttga aagttccgat
      721 cattcagcct atcgaatctt gcgcgctatc aaaaatcgtt ttggctccac caatgaaatc
      781 ggtgtcttcg aaatggccga aggtggatta agggaagttc aaaatccttc tgagttattt
      841 ttacaggaac gaaccatcga tgctcctgga acagccatcg tctcggcggt cgaaggttct
      901 cgtccaatat tggtagagct tcagacgctt gtgagccaat ctaccttggc catgccccga
      961 cgaacaacgt tggggatcga ccaaaataaa gtggcgctct tagtggcggt tattgaaaag
     1021 cgcttgggag tgccgctata tggccacgac attttcgcaa aggtagctgg cggcattaag
     1081 cttgatgaac cagcgaccga tttaggaatt gctgcggcat tgctcagtag tttcaagaac
     1141 cgagcgctcg acgctaaact attgatttgt ggcgaagtgg ggctaaccgg tgaagttcgc
     1201 cggatttcac agcttgatct gcggctttcc gaagcggcca aattaggttt tcaacgtgcg
     1261 atcgttccac aatctggatt agacgcactg gcaaaagcaa atttccgcgc aaaaatcgaa
     1321 gttaccgggg tacggtcgct cgatgaagca gttaaagcgc tagatctttg actaacttcc
     1381 gaataatcga acagctttga cggccctttt ccaacgaagc tccgacttct ttcgatcaag
     1441 tgatttgatt cggggttgaa atacacagtc ttttttccat gctttttcaa tcgccgaaaa
     1501 atcccgccaa acgcccgcac cgaggcctgc ggcgaaaacg gctcctagtg aggtggtttc
     1561 aataattttc ggacgtacaa ggctgcagtt taaagcatcg gcctgcatct gcatgagtag
     1621 cgtattttgg ctcgctcctc cgtcaacatt gagactgcgt agcggtagct tactgtcttt
     1681 tttcatggct tcgagaattt ccacattcat gtgtgcaatt ccttctagta ccgcgcgcgc
     1741 aacatgcgct ttggttgttc cacgagtaca cccgagcatg cccgcgcgtg cttcagggtc
     1801 ccaatatggc gcgccaattc cggtaaaggc cggtacgaaa atcactccgt cggtggtgtc
     1861 taccgtgcga gcgagcgctt cgatcgcagg agaattcgga attagttcca aaccatcgcg
     1921 cacccattgt actgcggcgc cggcagtgaa agcaccgccc tctagggcat aagttgaaac
     1981 gtcgttccac cgccacgcta ctgtcgtcaa cagtcggtgt tttgatcgca caatgcgagg
     2041 tccggtattc atcaaaatga acgaacctgt tccaaaggtg cacttcgctt gaccactttt
     2101 tacacaagcc tggcctagaa gggctgactg ctggtcaccc aaaacaccag taatcggaat
     2161 tccgttaggt agttctttaa caccgtaggt tttgccaaac gactcactaa acgaattaac
     2221 aacaagtggc agactatcta cgcttatttg aaagattctg cataaatctc tattccactc
     2281 attacttcgc aaatcaaaca acatggtccg cgaagcgttc gacggctcag taatatgaac
     2341 tcgaccaccg gttaggcgat gcaccaggta ggaatcgatt gtgccaaatg ctaactcacc
     2401 gcgctttgcc gctctggata cattcggatt gtgtttaaga gaccaaagaa ttttactggc
     2461 gctaaaatac ggatcaatca acaagccggt tgatttagaa atctgtggct ccagaccttt
     2521 ttttcgaagc tcgtcacaca aatcggtggt tcggcggtct tgccagacaa tcgcattgga
     2581 caacggctcg ccggtttttc gattccaaaa acacgaagtt tctcgctgat tagtgatgcc
     2641 aattcccacg atattgggag acttgcgctt aagcttggcc aacgcacctc gcattgccaa
     2701 agtcaccgac tgccaaatat gatccgggtt gtgctcaacc caacccggct tcggaaaaat
     2761 ttgcttaaag tcgacgtcgt gttgcgcgtg aatgttgaga tcggtgtcga ctattgatac
     2821 ggtcgacccg gtagttcctt gatcgatcgc aagtataagt tccatttaag tgaggatttt
     2881 ggccatgcgc gggttttttg gcaagcgaaa ggaaaagtta ttcgatctcc acaacctgga
     2941 tttcgataaa ctcgcattct atgtgctgcc gcgctttttg ctcgagattg ttcgtaagta
     3001 tttgcgattg gaaatcgaag gcgcagaaaa ccttccagca cggggtaaag ccattattct
     3061 accgaatcac tctggttggg cggggttcga tgcggttatg attgggaatg aaatttttaa
     3121 atcgaaaaaa cgaatcccga gaatcatggc ccacaagggt ttcttcataa ataccgaact
     3181 taaaattatt gcccgaaaaa tgggcatgga agaagcctcg actgacaatg gcgtgcggct
     3241 tcttaaaaag aacaacatcg taatgttgtt tccggaaggc gaatacggca actttaaacc
     3301 aactgccgaa cgctatcagc tccgcgaatt caagcggggt ttcgtaaaaa tggcacttca
     3361 ggccaacgcc ccgatcattc cgaccctggt aattggtgcg gaagagaccc acattaattt
     3421 ggcctcactt aaactgacca aatatcttaa gggtcaaatt attccaattc cgctgaacat
     3481 tctgccgctt ccggccaagt ggaaaattaa gtttctggaa ccaattaccc tcgaaaaata
     3541 cgctgatgtc gacccaaacg atggcgaaaa actcctgcaa attgcacaag aagtgcgagt
     3601 tcaaatgcaa gcacagctag acgctgaact caaaaagcgg aaatatgtgt attttccgac
     3661 tgaagatgag ggctccaata aaacggtcta acgaatttct tcgggcggtt cgcactttgg
     3721 gcaacccggg gcaactgttt ttccgatgtt catccacacc ccgccgatgc cgcgcagtac
     3781 cgaccggttg atgattgagt agatcccgag tatcgcgatc aacacaaacg cgtactccac
     3841 cagagcttga cccgactgaa atcgacggct tctcggccgt agcggccgaa atccttgaat
     3901 cataagatta attgtagtta gatagaccgg atgcggaaag cggttttcgg aattttaatt
     3961 ggatttgtct taattg
//
LOCUS       DAOMXO010000146         3975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12355_length_3975_cov_3.788520, whole genome shotgun sequence.
ACCESSION   DAOMXO010000146 DAOMXO010000000
VERSION     DAOMXO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12355_length_3975_cov_3.788520"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            518..1561
                     /locus_tag="PLH57_04160"
     CDS             518..1561
                     /locus_tag="PLH57_04160"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HRK01834.1"
                     /translation="MNFLWSILEDRLGKNFDTNAGVARAVLEVSRSFTENRKPELGKY
                     LENEAFHSGYLAYFLPLNAAKTEKLLTDHLKNAANAPRRVLDFGCGPGTAAIAYLSWY
                     AKQSLEDPVEITLVDHETHSLDAAQKLVRNVARKLGVEASVHTLPEIASDKAANHDLV
                     LAVNVLNELSANDRFFESLIAQEGTPRRKLLILEPSHRVASQKLIRLRKRLIETKTSS
                     FLKILGPCTHEKECPLFRSSRDWCHFSDRIADPRLVTMGRKIFKSSRDWMKYSYLFLE
                     QTSSAPVSKKNQDGELYRAIGDLHPTRRGTYAIDVCTPGKKIEWYYPKSIAPSKKLRL
                     FRGALIRREPTRQ"
     gene            complement(1532..2593)
                     /locus_tag="PLH57_04165"
     CDS             complement(1532..2593)
                     /locus_tag="PLH57_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRK01835.1"
                     /translation="MTFFVLLLMISFLGSALSLELIVRMTFPQPTGPVMFSYDPAIGV
                     IPKPSQSALRTLSEDPPYRASNDSEGLRVSWSANSDSSEKNRRRVLFLGDSYVYGSGV
                     SDEATFASVFAKITGYRAINAGVPGTGNDFALKFLKVRARAYRPDTVVIGFFPNDYAD
                     NMGGRFFEHRPDGTLSDVDVAASFAFYRNKQKLGEYRLYNWFCENSHAFNLARKLAAW
                     LKVSGGLGDVRAFLKNRPDMTLFERGFSNATNIEQAEQLFRIIRDEVRAIGAQLLVLY
                     IPTPVEVLNFRQTKQASRDELALVETLRKLDIELISTTAPLAESGIDQAQLYLNEGHW
                     NVEAHQIAGALLASRLTSN"
     gene            2766..>3975
                     /locus_tag="PLH57_04170"
     CDS             2766..>3975
                     /locus_tag="PLH57_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01836.1"
                     /translation="MFIVFKKSHWSWSLFDEVSWSQWYREGWNVEVFVNWDWDSIRRF
                     RPLSPFIVFERFRLLDLDPNMLRATRVLELILFSALYAICNIYVYRIHALVAGFLLVL
                     LLKSPPLLENLQILTLSELSGLTFVVVALLLYKKIKWLSYFFLLLAVLTKEPFMFFLA
                     LPALQNKRWREGITALVISAVYLGTLYMVRKGGYYDFGIHEGSTARVVDGYLRNFYPV
                     GLVLIASAPQIIKNLRSSKVDFQTVLGNFWSPLCTSLFSLLYASSVAFKAPGYTYHYG
                     PSVVLFALASALALNELLKKKLMLLPSLIIMAFSTIYFSAKPLRFIEKLHQEHLVRYT
                     LTEAIRNSGVKGPFVSNCEVDRKGLGYAADQREEGLNCMSQPRDLVLCCQGMSLIGII
                     DQCGEFDVLSK"
BASE COUNT         1063 a          944 c          882 g         1086 t
ORIGIN      
        1 aatttttttt actctttttc gaaaaaatag atgaaaataa tgaatttttt acactgtatc
       61 gagtttcgcc ttcgctaccc ggcgcagatc aacttttctc gtccttatca ggttcgatat
      121 ttgagttgga gttgggcgtg gggggctcag gagtcttatt tttggcttca aaaaatttgg
      181 ctacccgatc tcgcgcgagc ttaacgtcat atttgtagag ataggggtca aaaccacaat
      241 gggggcaaat cagactctct ctggccttaa atcggtaaac aaattcaaat atagcccaaa
      301 gcgggattcc caaaaaaact gttttaaaac cgaaccaccg ccacaatccc aacgtgaata
      361 ccagagttgt gataagaagg gccgcgtaat ggcgaatacg gggcgacggc caataaaatg
      421 accttcgttc ccgtttacaa atcgggcaat aaaaaaccca aaatgaacgt ttctgatact
      481 gaaaaggcag actgtccatc tgacaaaagt gtagcatatg aattttttgt ggagcattct
      541 cgaagaccga ctgggaaaaa acttcgacac caacgcgggt gtcgctcgag cagtgctcga
      601 ggtgagccgt tcatttaccg aaaatcgaaa accggagctc ggaaaatacc ttgagaacga
      661 ggcgtttcac tctgggtatc tcgcctactt tctgccactc aatgccgcca aaactgaaaa
      721 acttttgacg gaccatctta aaaacgctgc aaatgcacct cgtagagtcc tcgatttcgg
      781 atgtggtccc ggcactgctg ctatcgccta tcttagctgg tacgctaaac agtcgctcga
      841 agatcccgtc gaaatcacct tggtagatca cgaaacccat tcccttgatg cagcgcaaaa
      901 acttgtacga aatgtggccc gaaaactggg cgtcgaagcg tcggtgcaca cgcttccgga
      961 aatcgcttcc gacaaggccg caaatcatga tcttgtactc gcggtaaatg ttttaaatga
     1021 attgtcggcc aacgatcgtt ttttcgaatc cttgattgct caagaaggaa ctccgagacg
     1081 aaaacttttg attttagaac ccagtcaccg agtggcttct caaaaactta ttcgtttgcg
     1141 caaacgattg atcgaaacta aaacctcgag ctttttaaaa atattgggac cgtgcactca
     1201 cgaaaaagag tgcccgttat ttagaagttc gcgcgattgg tgccattttt ccgaccgcat
     1261 tgccgacccg cggctagtca ctatggggcg caaaatattt aagtctagtc gcgactggat
     1321 gaaatattct tacctttttc ttgaacaaac ctcttcagca cctgtcagta agaaaaatca
     1381 ggacggcgaa ctttaccgcg ccattggcga cctccaccct acacgtcgcg gaacctatgc
     1441 gatcgacgta tgcactcccg gaaaaaaaat tgagtggtat tatccgaagt cgatcgcacc
     1501 atcgaaaaag ctgcgccttt ttcgtggcgc tttaattaga cgtgagccta ctcgccagta
     1561 gggctccggc aatttgatgg gcttctacgt tccagtggcc ttcgttcaaa tataactgcg
     1621 cctggtctat accgctctcg gccagtggtg cggtggttga gataagctct atgtcgagtt
     1681 ttctcaaagt ttctaccaaa gccagctcat cacgactcgc ttgtttggtt tgtcggaaat
     1741 ttaacacctc gacgggagtc ggaatataaa gcaccaacaa ttgggcacca atcgcgcgga
     1801 cttcgtcgcg aataattcga aaaagttgtt ccgcctgctc tatatttgtc gcatttgaaa
     1861 aaccccgttc aaaaagtgtc atgtcggggc gattttttaa aaaagcacga acatcaccta
     1921 gcccccccga tactttgagc cacgccgcaa gctttcgagc aagattgaag gcgtgcgagt
     1981 tttcgcaaaa ccagttatag agacgatatt caccgagctt ttgtttgttc cgataaaaag
     2041 cgaacgaagc tgccacgtcc acatccgaaa gtgtcccgtc ggggcggtgc tcaaaaaaac
     2101 ggccccccat gttatcggcg tagtcatttg ggaaaaaacc tatcaccact gtatcggggc
     2161 ggtaggcgcg agctctaacc ttgaggaact tcaaagcgaa atcgttgccg gtccccggaa
     2221 cccctgcgtt aattgcccga taacccgtaa tttttgcgaa tacgctggcg aacgtagcct
     2281 cgtcacttac ccctgatccg tacacgtaag agtcacctag aaaaagaacg cggcgtctgt
     2341 ttttttcaga actgtctgag tttgcagacc aagacactct caacccctcg ctgtcgtttg
     2401 aggcacgata gggggggtcc tctgataaag tgcgtaaagc tgactgactt ggctttggaa
     2461 tcactccgat tgcgggatca taactaaaca tcactggtcc ggtaggctgc ggaaaggtca
     2521 tccgcacgat taactcgagg cttagagctg aacctaaaaa agagatcatc aaaagtagaa
     2581 cgaaaaaagt caatttccgc cgacgcgtca tagccgccaa gttaagcgca aaggtcgggg
     2641 ttgtcgaagt gaaaatgcgt tcgtagagtc gtccaatgct ccagattcac aatctaatcg
     2701 caaagcgtct actccaaatc aggtggactt acttcattcc caccttcgtt gggctaattt
     2761 tttctatgtt catcgttttt aaaaagagtc actggagctg gtcgcttttt gacgaagtca
     2821 gttggtccca gtggtacagg gagggttgga acgttgaagt attcgtaaac tgggactggg
     2881 attctattcg tcgatttcga ccacttagcc catttatagt ctttgaacga ttccgcttac
     2941 tcgatctcga ccctaacatg cttcgagcaa cacgggttct agaactcatt ttatttagtg
     3001 ctctttacgc aatttgtaat atttatgttt atcggattca cgcacttgtt gcgggttttt
     3061 tactggtgtt attgctcaaa tcaccgccat tgcttgaaaa cctacaaatt cttacgctaa
     3121 gcgaactctc gggcctaacc tttgtagtcg tcgccttgct actttataaa aaaattaaat
     3181 ggctcagtta ctttttctta cttctggcag ttctcaccaa agaaccattt atgttttttc
     3241 ttgccctgcc cgccttgcaa aataaaagat ggagagaagg aatcactgcc ctagttattt
     3301 ccgcagtcta ccttggaaca ctttatatgg tgagaaaagg cggttactac gacttcggaa
     3361 ttcatgaggg atcgacagca cgagtagtcg atggttatct taggaacttt taccccgtag
     3421 ggttggtact gatagcttcg gcacctcaaa tcattaagaa tctccgtagt tctaaagttg
     3481 atttccaaac agttttaggg aatttttggt cgccattatg tacgagttta ttttcactgc
     3541 tttatgcttc cagtgtcgca tttaaggcgc ccggatatac ctatcactac ggccccagtg
     3601 ttgttttatt tgctttggcg tcggctcttg cactcaatga gttactgaaa aaaaagttaa
     3661 tgctcttgcc tagtttaatc ataatggctt tttccacgat ctatttttct gcgaaaccgt
     3721 tacgctttat cgaaaaactc catcaggaac acctggtaag atacaccctc actgaggcca
     3781 ttcgaaactc aggtgtaaaa ggtccattcg tttcaaactg tgaagtcgac cgcaaaggtc
     3841 tcggttacgc tgctgatcaa cgcgaagaag gattaaattg tatgtcacaa cctcgtgact
     3901 tggtgctttg ttgccagggc atgtcgctta ttggaatcat agatcaatgc ggcgaattcg
     3961 atgtactttc gaaac
//
LOCUS       DAOMXO010000147         3956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12436_length_3956_cov_5.068700, whole genome shotgun sequence.
ACCESSION   DAOMXO010000147 DAOMXO010000000
VERSION     DAOMXO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12436_length_3956_cov_5.068700"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLH57_04175"
     CDS             <1..195
                     /locus_tag="PLH57_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01837.1"
                     /translation="KAGTVRMRELLEKFPARSAIHQASQEILNKEFVTFFENNRWKPL
                     AAELAARFEKWDKKLEESPK"
     gene            192..1043
                     /gene="panB"
                     /locus_tag="PLH57_04180"
     CDS             192..1043
                     /gene="panB"
                     /locus_tag="PLH57_04180"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRK01838.1"
                     /translation="MTYLANSAPTNGIRKRVTAPWVRAQKNLCKLSMLTAYDFAFATL
                     LDRAGIDMLLVGDSLGPVIYGTPNTLSVTLDDILRHTRTVSSAATRALVIADLPFLSY
                     QVSLEQAVTNAGRCLKEAGAHAVKLEGGVEQTETIRAIVRAGIPVVAHIGLTPQSVHA
                     VGGYRTYGKTEAERDLLLADAKAVAEAGAFCVVLECVQEATATEITKAIAVPTIGIGS
                     GSNCDGQVLVTQDLVGLTVGHVPKFVNPVADVAGIISAAAQSFVARMNDGEESKPVVK
                     GENVISH"
     gene            1027..1812
                     /locus_tag="PLH57_04185"
     CDS             1027..1812
                     /locus_tag="PLH57_04185"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRK01839.1"
                     /translation="MLLAIDVGNSHTVFGCFWDGDLKKKWRVSTRPFGTGDELRLKTE
                     SFLKLEGISIRAFEAIIASSVVPQMTEVLRNAFGDLNFRVIDHTWNFSFANTASPASS
                     VGADRLVNAETAVREFGAPCIVVDSGTATTICAVGGSSTKPLYLGGAIMPGIELSMEA
                     LASRAAKLFSIELTAPECAIGNDTTTAMQSGILFGYAEMVDGMVRRFKNEMQSKNAKV
                     IATGGVSRLLRGVTREIDAFEDDLTLRGIYYLYDAVRANLRDR"
     gene            1778..3100
                     /gene="coaBC"
                     /locus_tag="PLH57_04190"
     CDS             1778..3100
                     /gene="coaBC"
                     /locus_tag="PLH57_04190"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRK01840.1"
                     /translation="MTPSAQTFVIDNCRHGLVGKKILLGVTGSIAAYKTPELVRLLKK
                     AGAEVRVVLTPAGKNFVAPLALSTVSESPVMESMWSDAIDTHHITAARWADLLLIAPA
                     TANVIAKLANGICDDLLTTESLAFTGPILIAPAMNPAMWSAAATQENVARLKNRGVQI
                     LGPAVGETSCKEEGAGRMIEPIDLLIQVAMAASAADQKTQSPTRARVLLTLGPTRSFA
                     DPVRFLTNRSSGKMGAALAWAALFSNCDITCIVGPTDIELPPVGTPRVRVLRVDTATE
                     MHRAAMELWPHHDLFIGTAAVLDWEFSTTETEKIKKQDSTSSEQWQFTLRTAPDILKG
                     VSQAARADQFTLGFAAETNDVIRYARTKRVAKTCDAIFANDVSSGGFESDTNEGVWID
                     QFTDVAFAAQKKTELAAQLLVLALQKWHYKKSLIKQSQEALRGHSANT"
     gene            3078..3926
                     /gene="panC"
                     /locus_tag="PLH57_04195"
     CDS             3078..3926
                     /gene="panC"
                     /locus_tag="PLH57_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRK01841.1"
                     /translation="MVILQTPKQFQQYRRTIPHGQSVGFVPTMGALHAGHAELLKRSA
                     AENQVTILSIFVNPKQFGPREDFQKYPRPFEADLDIAEACGVSAVFSPSVADFYPDSF
                     STYVEENALSKPLCGAFRPGHFRGVTTVVLKLFNVVRPNRAYFGLKDAQQFFVIKKMV
                     EDLNLDLEIKGVPTVRENDGVALSSRNRYLSSEERERAPMIFKTLTRARDQILSGADP
                     AKVCCEQIAVLEQNGFRVQYFEYWPQGRVLAAAAYLGITRLIDNVLIETTKQTRVSEA
                     SPESTI"
BASE COUNT          977 a         1056 c          997 g          926 t
ORIGIN      
        1 aaagcaggca ccgtacgaat gcgcgagtta cttgaaaaat ttccagccag atcggccata
       61 caccaagcct cccaagaaat tctcaataaa gagtttgtta cattctttga aaacaaccga
      121 tggaagccgc ttgccgccga acttgcagcg cgcttcgaga aatgggataa gaaactcgag
      181 gaatccccca aatgacttat ctcgcaaata gcgctcccac aaacggtatc cgaaaaagag
      241 tcaccgcgcc ttgggtgcgt gcccagaaaa atttgtgcaa actttccatg ctcacggcct
      301 acgattttgc gtttgccacc ttactcgacc gcgcaggcat cgacatgctg cttgtgggtg
      361 acagtcttgg ccccgtgata tacggcacgc ccaacacact atctgtcaca ctcgacgaca
      421 ttttgcgcca cactcgaacg gtttcaagcg ccgctactcg tgcactcgta atcgcagatc
      481 ttccgttctt aagttatcaa gtcagtctcg agcaagcggt caccaacgct ggtcgctgcc
      541 taaaggaagc gggagcccat gcggttaagc tcgaaggcgg tgttgaacaa accgaaacga
      601 ttcgcgccat cgtgcgagcg ggcattcccg ttgtggctca catcggcctt actcctcaga
      661 gcgtacatgc ggtcggcggt taccgcacct atggcaaaac tgaggcagag cgtgacttgc
      721 tgttggcgga cgccaaagcg gtcgcagaag caggcgcttt ttgcgttgta cttgagtgcg
      781 tacaggaagc aacggcaact gagatcacca aggctatcgc ggtcccaaca atcggaatag
      841 gatccgggtc caactgtgat ggacaggtgt tagtgaccca agacttggtc ggtttaaccg
      901 tagggcacgt accaaaattc gttaatcccg ttgccgacgt tgccggaatc atttcggctg
      961 ccgcacagtc cttcgtcgcg cgaatgaacg acggcgagga atccaagccc gttgtgaaag
     1021 gcgaaaatgt tattagccat tgacgtcgga aactctcaca ctgtctttgg ctgtttttgg
     1081 gacggtgacc tgaaaaaaaa atggcgtgtt tctacccggc cctttggaac gggagatgaa
     1141 ttacgcctta aaaccgagag ttttctaaag ctcgaaggaa tttctattcg cgcattcgaa
     1201 gcgataatag cctcgagcgt ggttcctcaa atgaccgaag ttctgcggaa cgcttttggt
     1261 gatctgaatt ttcgtgtcat tgaccacact tggaacttct catttgccaa cacggctagt
     1321 cctgcgtcga gtgtgggcgc tgatcgttta gtgaacgccg agaccgcagt gcgcgaattc
     1381 ggagctcctt gcatagtggt cgactcgggc accgccacca caatatgcgc cgttggcggt
     1441 tcatcgacga agccgctata tttaggtggc gctatcatgc cggggatcga gttatcgatg
     1501 gaagcgctcg caagccgtgc tgccaaactt ttttcaattg aactcacggc acctgaatgt
     1561 gctatcggca acgacactac gaccgccatg caaagcggca ttctctttgg ttatgctgaa
     1621 atggttgacg gcatggttcg tcgctttaaa aacgaaatgc aatccaaaaa cgctaaggtg
     1681 atagccactg gcggagtgag tcgtttactc cgtggagtga cacgcgaaat cgatgcattc
     1741 gaagatgatc taactttaag aggaatttat tatctctatg acgccgtccg cgcaaacctt
     1801 cgtgatcgat aactgccgcc acgggctcgt tggaaaaaag atcctgctcg gggttaccgg
     1861 cagtattgca gcctataaaa cgcccgagct cgtgcggttg ttgaaaaagg ccggcgccga
     1921 agtacgcgtc gtacttacgc cggctggcaa aaattttgtc gctcccttgg cgctttcgac
     1981 tgtttcggag tcgccagtaa tggaatcaat gtggtcggat gcgatcgaca ctcaccacat
     2041 cacggcggct cgttgggctg atcttttatt gatcgcacct gctactgcta acgtgattgc
     2101 aaaacttgca aatggaattt gcgacgacct actcacaacc gaatcactcg cgttcacggg
     2161 acccatttta attgcgccag ccatgaatcc tgcgatgtgg tcggcggcag cgactcaaga
     2221 aaacgtcgcg cgtttaaaaa atcgcggtgt tcaaatttta ggcccggcgg tcggcgaaac
     2281 ttcctgtaag gaagagggcg cgggccgtat gatcgagccg atcgacttac tcattcaagt
     2341 cgccatggcc gcttccgctg ccgaccaaaa aacacagtct cccactcgag ccagagttct
     2401 gctcaccctt gggccgacga gatcttttgc cgaccccgtg cggtttctca ccaaccgatc
     2461 tagtggaaaa atgggagccg cactcgcctg ggccgctctg ttttcgaatt gcgatatcac
     2521 ctgtattgtc ggtccgacag acatcgaact gcctcccgtc ggcacccccc gcgtgcgtgt
     2581 attgagagtc gatacggcca ctgaaatgca ccgagctgcc atggagcttt ggcctcatca
     2641 cgacttgttt atcggtactg ctgccgtact ggactgggaa ttttccacaa cagagactga
     2701 aaaaataaaa aaacaagatt ctacgtcaag cgagcagtgg caatttactt tgcgtactgc
     2761 gcccgacatt ttaaagggcg tttcgcaagc cgcgcgcgcc gaccaattca cccttggttt
     2821 tgccgctgaa accaacgatg taatcagata tgctcgaact aaacgcgtcg caaaaacttg
     2881 tgatgcgatc tttgccaacg atgtttcatc tggcggcttc gagtccgata ccaacgaagg
     2941 ggtctggatt gatcaattca cggacgtcgc atttgccgct cagaaaaaaa cggagctggc
     3001 cgcacaatta ctcgtattgg ccctacaaaa atggcattat aaaaaatcgt taatcaaaca
     3061 atctcaggag gcgctccgtg gtcattctgc aaacacctaa acagtttcag cagtaccgcc
     3121 ggacgatccc ccacggccag tccgtcggat tcgtaccaac aatgggggct ttgcatgccg
     3181 gacatgccga attactgaaa cgctcggcgg cagagaacca ggtaacgatc ctatcaattt
     3241 tcgttaatcc caaacaattc ggaccgcgcg aagactttca aaagtacccg cgcccattcg
     3301 aagcggacct cgacattgct gaagcttgtg gggtttcagc agttttttca ccatcagtcg
     3361 ccgactttta ccctgattct ttttcaactt acgtcgaaga aaacgctctc tcgaagcccc
     3421 tgtgcggggc ctttcgccct gggcatttcc gcggggtaac gacggtagtg ctaaaacttt
     3481 ttaatgtagt acgtccaaat cgtgcttatt tcggcctaaa agacgcccag caatttttcg
     3541 tcattaaaaa aatggttgaa gacctcaatc ttgatctcga aatcaagggc gtacccaccg
     3601 tgcgcgaaaa tgacggcgtg gcacttagtt cacgcaaccg atatttatcg agcgaagaaa
     3661 gagaacgagc gccgatgatt tttaaaaccc tcactcgcgc ccgcgatcaa attctaagcg
     3721 gcgcggaccc tgcgaaggtt tgttgcgaac aaattgcggt actcgagcaa aacggctttc
     3781 gcgtgcagta ttttgaatat tggccgcaag gccgcgtgct cgcggccgcc gcttatttag
     3841 gcattactcg tctgattgat aacgtgttga tcgagactac caaacaaact cgcgtgtcgg
     3901 aggcgagccc ggaatcaacg atttaattgt cagttcagga gcgtcgaacg cctcgt
//
LOCUS       DAOMXO010000148         3954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12444_length_3954_cov_4.502437, whole genome shotgun sequence.
ACCESSION   DAOMXO010000148 DAOMXO010000000
VERSION     DAOMXO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12444_length_3954_cov_4.502437"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1372
                     /locus_tag="PLH57_04200"
     CDS             302..1372
                     /locus_tag="PLH57_04200"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRK01842.1"
                     /translation="MSLTLDSVMGALRGVRGLPMGMVKDLKITDRNVSLKINLAGANP
                     DVVKEIELRTRQALTNSGASSVDLMIDGKVAATAPKTDAQFNKHSVPGVKHIIAVSSG
                     KGGVGKSTVASNLAIALKKLGHKVGLLDSDVYGPNIPTMMGSLEPPMARQDATRGELI
                     LPPEAHGVKIMSMGTLNPGDQPLVWRGPMLHSIVNQFLMKVDWAPLDYLIIDMPPGTG
                     DVQLSLTQLVPVSGAVVVTTPQEVAMQDVRKAIFMFEKVSVPVLGVVENMSYFVCGKC
                     DEKHYIFGENGAEKLAEKFNIPVLAKLPLHPSVREGGDTGVPIAAMLPEGPHAAAFAE
                     LGRAVVAAVQKTEAAELEVTGF"
     gene            1455..3236
                     /locus_tag="PLH57_04205"
     CDS             1455..3236
                     /locus_tag="PLH57_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01843.1"
                     /translation="MRFLFSKKNTQLAAIITLLIVVGGDASAACKDVLLNRALGLEWE
                     LGDIRRERVITEADGTKLLRYILLERSKIQDLYGFFGRAESENPNSPWIRNFLPQRVA
                     EHPEVINELARLGLRIEQGVLTGLQRLSGQDIHSLVKTLFEARGKFSRDLRLRKVPEA
                     WLPKVGEVLTKEQHALDDLIAMELAEEWMIDASGNNVFLTVEFPSERPFTNREELVAY
                     LQHFVARLELLPPEKNTQINVLRWLAGHIHHTFDIAQVDDRYVRETYYRSLVRMWGDL
                     NDLDIVSYLNLMSIGSDYLSGELPLTRSQFVSLAEQLEGMFQNRTFSILTREMYERLD
                     ITDRGVVVRERDKGLDPFFDVNFPVVYKRLSYGLRGNYGQATVPAGEPVPLVGIEARG
                     HRAFGDAIASVPEMAAKILDHDQAGPRGLLSDSDLLRRKASELGIDSDFMVDAEHVLA
                     QGTYRWTPEQVRAMVFLPLNDWLKHPLAKKNLQAMGSYTRVRAEANFEVALSRYVVRM
                     NELANARKRLEYIYRPWKNYVRERLGDFNIVNRSHQYMDLERANSGYSYAEYADDWFR
                     KVLILESARFVSEAQLAAMLGAKDDSE"
     gene            complement(3241..3468)
                     /locus_tag="PLH57_04210"
     CDS             complement(3241..3468)
                     /locus_tag="PLH57_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRK01844.1"
                     /translation="MADKNKKWEDNVPGKFFVDDQCIDCDACRTEAPSNYTRNNSHGY
                     SYVFKQPTTPEEEKACQAALEACPVEAIGKE"
     gene            3526..>3954
                     /locus_tag="PLH57_04215"
     CDS             3526..>3954
                     /locus_tag="PLH57_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01845.1"
                     /translation="MQRIKLVISDCHLSAGLNFESQRNPHEDFLFDDEMIAFFEYFSS
                     GPYGDQCEVELIINGDYFDFLNVPYKGEFEEIITEEFALYKLECILAGHPRVIDALRR
                     FAARERKTIIYNIGNHDADLFFPKVRERLVRALDPDGQFPS"
BASE COUNT         1011 a          941 c         1004 g          998 t
ORIGIN      
        1 gtggccgacg attccgaact gacccaactg cgcgaccgct ggggccacca gatgttgaga
       61 ctcggcgatt tactgaaaga aggattgatt agtaggtccc aaaggacgaa aaaatataaa
      121 aatcccaggt tcatatcgaa caatgatcga catattttta gggccgaata agcctggaaa
      181 actctgtcca ggtgcaattt tacgcacagc gagggcttca aataaaaagc aattttaagt
      241 gatgaccccg ctcaaagttg cgatataagg ggaacacaag actaggaaag ggacctaaac
      301 gatgtcactc actctcgatt cagtaatggg cgcgctcaga ggcgtgcgtg gactcccgat
      361 ggggatggtc aaagatttga aaattaccga ccgcaacgtg tctctaaaaa ttaatttagc
      421 gggcgcgaat ccggacgttg taaaagaaat cgaacttcgc acccggcaag ctcttacaaa
      481 cagcggcgcc tcgagtgtgg atttaatgat cgacggaaag gtcgcagcga ctgcgcctaa
      541 aaccgacgct cagtttaaca aacattctgt tcccggagta aaacacatta ttgcggtttc
      601 tagcggcaag ggcggcgtgg gtaaatcgac cgtcgcttcg aaccttgcga tcgcacttaa
      661 aaaactgggt cataaagtag gcttgctcga ttctgacgtg tacggcccga acattcctac
      721 tatgatgggt tcactcgagc ccccgatggc gcgccaagac gcgactcgcg gcgaattgat
      781 tctgccgccc gaagcacacg gcgtaaaaat tatgtctatg ggtacactca atccaggtga
      841 tcagccactc gtttggcgag gcccaatgct tcatagcatt gtgaatcagt ttttaatgaa
      901 agtagattgg gctccactcg attatttgat catcgacatg ccgcccggca ctggtgatgt
      961 gcagctttcg cttacacaac ttgtacctgt tagtggtgcg gtagtcgtaa ctactccgca
     1021 agaagtcgcc atgcaagacg tgcgcaaagc catattcatg ttcgaaaaag ttagtgtgcc
     1081 tgttctcggc gttgttgaaa atatgagtta cttcgtttgc ggcaagtgcg acgagaaaca
     1141 ctatattttt ggagaaaacg gggcagaaaa attagcggag aaatttaata taccagtttt
     1201 agctaaactc ccgctgcacc caagtgtgcg tgaaggcgga gatactggcg taccgattgc
     1261 cgccatgcta ccagaagggc cacacgccgc ggcatttgct gagttgggcc gagcggttgt
     1321 ggctgcagtt caaaaaactg aagccgcaga gcttgaagtc acgggttttt aggcccatag
     1381 agccacgcga aacctatttc cgatgctttt aatatagtat tgaaaaccgc tcgccagagt
     1441 gttaatgcgg tgcaatgcga tttctgttct ctaaaaaaaa tacgcaacta gctgcaatta
     1501 ttacgctttt aattgtagtc ggcggcgacg cctcggccgc ctgcaaggac gtgttgctga
     1561 atcgcgctct cggtttagag tgggaacttg gcgatattcg acgtgagcga gtgattacag
     1621 aggccgatgg tacaaaactg cttcgttaca tacttttgga gcgctcaaag attcaagatt
     1681 tgtatggttt ttttggccgc gccgaatccg aaaatccaaa ttccccctgg attcgtaatt
     1741 tcttgcctca gcgagtagcg gaacaccccg aagtcataaa tgaattggcc cgattggggc
     1801 ttcgaatcga gcagggggtg ctcacagggt tgcagcggct ctctggccaa gacattcact
     1861 cactcgtaaa aactttgttt gaagcccgcg gcaaattttc tcgtgacctt cgattgcgca
     1921 aagtgcccga agcttggctt cccaaggtcg gggaagttct taccaaagag cagcacgcgc
     1981 ttgatgatct tatcgccatg gaattagccg aagaatggat gatcgatgcc agtggcaaca
     2041 atgttttttt gacggtggaa tttccttcgg agcggccctt cacgaatcga gaagaattgg
     2101 ttgcttacct ccagcacttc gtggctcgtc tcgaactttt acctcctgaa aagaataccc
     2161 aaattaatgt attgcgctgg cttgccggac atattcacca cacgtttgac attgcccaag
     2221 ttgacgaccg ctacgtgcga gagacctatt atcgctcctt ggtgcgaatg tggggcgacc
     2281 tcaatgatct cgatatcgtt tcctatttaa atctaatgtc cattggaagc gactacctca
     2341 gcggtgaact tccgttgact cgttcgcagt tcgtcagttt ggcagaacag cttgaaggca
     2401 tgtttcaaaa tcgtactttt agcattttga ctcgcgagat gtacgaacga ctggatataa
     2461 ccgatcgagg tgtcgtagtt cgagaacgtg ataaagggct tgatccgttt ttcgacgtga
     2521 actttccggt tgtctataaa cggctttctt atggccttcg cggcaactac gggcaagcaa
     2581 cagtgcccgc cggtgagcca gttccactcg taggaataga agctcgcgga catcgggcat
     2641 ttggcgatgc aatcgccagt gttcctgaaa tggcagctaa aattttagat catgatcagg
     2701 cgggtcctcg gggcttattg tcggactctg atttgcttcg ccgcaaagca agtgaacttg
     2761 gaattgattc agatttcatg gttgatgccg aacatgtctt ggcgcagggg acctatcgct
     2821 ggacccccga acaggtgcgg gcaatggttt tcttaccgct caacgactgg ctgaagcacc
     2881 ctttggcgaa aaagaattta caagcgatgg gttcctacac tcgcgtgcga gccgaggcta
     2941 atttcgaagt ggcactgagt cgctacgtgg ttcgtatgaa cgaactcgcg aacgcccgca
     3001 aacgattaga gtacatctat cgaccgtgga aaaattacgt gcgagaacga cttggagatt
     3061 tcaatattgt aaaccgctca catcaatata tggaccttga acgcgccaat tcggggtaca
     3121 gttacgccga atacgcagac gactggtttc gaaaagtctt aatattggag tccgcccgtt
     3181 ttgtatctga ggcgcagctg gcagccatgc tcggagcaaa agacgattcg gagtaaaatg
     3241 ctattcttta ccgatagctt caaccggaca ggcttcgagg gccgcctgac aggccttttc
     3301 ttcttccgga gtggttggtt gcttgaaaac gtaggaataa ccatgcgaat tattgcgcgt
     3361 gtagttgctt ggagcctccg ttcggcaggc atcacagtcg atacactggt cgtctacgaa
     3421 aaatttgccg ggcacgttgt cttcccactt tttatttttg tccgccatcg tcgatttcac
     3481 ctaaaatagt ttaataagct tttctgtaga cctatacact agcgaatgca acgaatcaaa
     3541 ctagtcataa gcgactgcca cctttccgcc ggtctgaact tcgagagtca gcgaaatccg
     3601 cacgaggatt tcctgttcga cgacgaaatg atcgcatttt tcgaatactt ctcgagcggg
     3661 ccctacggcg accagtgtga agtagaactc atcataaatg gggactattt cgatttccta
     3721 aatgtcccct ataaagggga gtttgaagaa atcatcaccg aagagttcgc tctctataag
     3781 ctcgagtgta tcttagcggg ccatccgcgg gtaatcgacg cattgcgtag gttcgcggcg
     3841 cgggaacgta aaacaatcat ttataatatc ggcaatcacg acgctgatct ttttttccca
     3901 aaagttcgcg aacgcttggt gcgggcgctc gaccctgacg gccagtttcc gagc
//
LOCUS       DAOMXO010000149         3943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12489_length_3943_cov_4.531636, whole genome shotgun sequence.
ACCESSION   DAOMXO010000149 DAOMXO010000000
VERSION     DAOMXO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12489_length_3943_cov_4.531636"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLH57_04220"
     CDS             <1..440
                     /locus_tag="PLH57_04220"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRK01846.1"
                     /translation="LSSCEAKSPTRELRSAFMKNFGVKIRVEFPAAHHIRGYEGECAR
                     PHGHNFKVEVEATSPGISNIGIALDFRDLKEMTKELANRFDHQDLNTIAPFTDINPTA
                     ETLAQYFFEELERLVQRNPKTQTVTMKRVTVWENDRSAASYGY"
     gene            443..1183
                     /gene="queC"
                     /locus_tag="PLH57_04225"
     CDS             443..1183
                     /gene="queC"
                     /locus_tag="PLH57_04225"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRK01847.1"
                     /translation="MRKAVALLSSGLDSAANLALANEAGIDVQLALTFDYGQRAAVKE
                     IESAKRLSDYFGISHLVVNLRNFPEWLGSSGSALLDEGSMPSPESLDDLAVIRKSAKA
                     VWVPNRNGVFISIAAAAAEARDASVVLVGFNAEEAVTFPDNSRAYISAMNASLEFSTA
                     NRVKVESLTSAWNKKEIVARLLEFSPSFPNGRFPFEAIWSCYVAENQHCGICESCQRL
                     RRALLACDAVGVANKIFTKNRDDGVPSA"
     gene            1164..1748
                     /locus_tag="PLH57_04230"
     CDS             1164..1748
                     /locus_tag="PLH57_04230"
                     /inference="COORDINATES: protein motif:HMM:NF015950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF366 family protein"
                     /protein_id="HRK01848.1"
                     /translation="MEYPVLSQVKARVLDKPLFYTGVELRPHWCLEQTGVYGSVITAW
                     VGPCRVATSELVDMEDRLAAERIEAKEMLHFIAEFYGITLEAGVLYQRLFVQWCERLL
                     REAGMQNLRREGDDLLTPHGKLSVSIATVSPVSVLIHWALNIDGTGAPVPTAQLADLG
                     WGREQSLGFAKKVLEHYIGEIEEIRIAQCKVRPV"
     gene            1720..2844
                     /gene="hisC"
                     /locus_tag="PLH57_04235"
     CDS             1720..2844
                     /gene="hisC"
                     /locus_tag="PLH57_04235"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRK01849.1"
                     /translation="MPNAKYVPFDLKLDLSEHLKKLKPYVSGKPIEQTKREFGLSEVI
                     KLASNENPTGASPRALEAVRKTLEQSHLYPDASYQSLKNAIAKKRMLDPNQVVVGAGS
                     NEIIDLAIRVLLHPGQNCVAPAYSFIAYQLCAELNGVEFRTAKLDADFAISVDQILAV
                     VDANTRVVFLANPNNPTGITLEAKELRRLASGLLKKNVILCLDYAYWEFADSKDFPAA
                     EDLLVDYPNLLVLHTFSKIYGLAGFRVGYGLGRPELIQWIERARQPFNVSSIAIEAAI
                     AALEDQAFLIRVKEQNAKERERLAMALSRLGLRVYGSNANFVFVDLHRDAEQVNSELL
                     KRGVIIRPLLNYGLKTQVRVTVGLPQENDKLLKALKEVLV"
     gene            2841..3551
                     /gene="cmk"
                     /locus_tag="PLH57_04240"
     CDS             2841..3551
                     /gene="cmk"
                     /locus_tag="PLH57_04240"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRK01850.1"
                     /translation="MSESRFVVAIDGPAGSGKSTVTHLLAKRLGFAHIDTGALYRAIA
                     YRATQMKTSLDDALALGKIATSAKLSFQWLNDRNQIFLDGENVSSMIRTPEISMAASK
                     ISAYAPVRDALLQLQRDLGARPPGAVLEGRDIGTVVFPNAEIKFYLNASVDSRARRRM
                     VELEEKGTFLDFEEVKRQVVERDKGDMERKVAPLKKADDAIEVDTTNMNVEQVVDFLF
                     ALVQERRGLAQSGRSKGR"
     gene            3553..>3943
                     /locus_tag="PLH57_04245"
     CDS             3553..>3943
                     /locus_tag="PLH57_04245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRK01851.1"
                     /translation="MRLLVVRPDRIGDVVLSTPALQLLKAAIPDAEVHVLVNKNVAPV
                     LAQLPFVSRIISPPSSGSAMDRLRKLSQMLGAERYDLAIVLQVEPLSTLAVRLARIPN
                     RVGPRSKFYSYMAFNYGLKQRRSDSKQN"
BASE COUNT         1014 a          896 c         1035 g          998 t
ORIGIN      
        1 tattatcatc ttgtgaagca aaatctccca ctcgtgaatt gagatcggca tttatgaaaa
       61 actttggtgt caaaattcga gtcgagttcc cagccgcgca ccacattcgg ggttacgaag
      121 gcgagtgcgc ccgcccccat gggcacaatt ttaaggtcga agttgaggcc accagtccgg
      181 gtatcagtaa tatcggaatt gcactcgact ttcgcgatct aaaagaaatg actaaagagt
      241 tagcaaatcg ctttgatcat caagatttaa acaccatcgc cccgtttacc gacattaacc
      301 caacagctga gactctagca cagtactttt ttgaagagct agaacggcta gttcagcgca
      361 accctaaaac tcaaacggtt acgatgaagc gagtgactgt ttgggaaaac gaccgttccg
      421 ccgcgagtta cgggtactaa aaatgcgaaa ggcagttgca ctactttcga gtggactcga
      481 ctcggccgca aaccttgcat tggcaaacga agcgggcatc gatgttcaac tagctttgac
      541 tttcgactac ggtcaacgag cggcggtcaa agaaatagag tcggcgaaac ggctttctga
      601 ttattttgga atctcccacc tagtagttaa tttaagaaat tttcctgagt ggttgggatc
      661 cagtggtagc gcgctcttag acgaaggttc gatgccgtca cctgaatcac tcgacgatct
      721 ggcagtgatt cgaaagtccg caaaagctgt ctgggtgcca aaccgtaacg gtgtatttat
      781 ttcgatcgcg gccgcagcag cggaggcccg tgacgcaagt gtagttttgg ttggatttaa
      841 cgcggaagaa gccgtgacct tcccggataa ttcccgagcg tacatttcag caatgaatgc
      901 ttcactcgaa ttttcgactg ctaatcgtgt aaaagttgaa agcctgacca gcgcgtggaa
      961 caaaaaagaa atcgtcgcgc gtctgttgga gttttcacct agcttcccga atgggagatt
     1021 tccttttgaa gcgatttgga gttgttacgt tgcggaaaat caacattgcg ggatttgcga
     1081 gtcctgccag cggctgcgtc gcgctttgct tgcttgtgac gctgtaggcg tggcgaataa
     1141 aattttcact aaaaaccgcg acgatggagt acccagtgct tagtcaggtg aaggcacgcg
     1201 tgcttgataa accgcttttc tacactggcg tggaactgcg gcctcattgg tgtctggaac
     1261 aaacaggtgt ctatgggtct gttatcacgg cctgggtggg cccgtgtagg gttgctactt
     1321 cagagctcgt tgatatggaa gaccgcttgg ctgccgaacg cattgaagcg aaagaaatgc
     1381 ttcattttat tgcggagttt tacggaataa cgcttgaagc cggagtgctc taccagagac
     1441 tttttgtgca atggtgcgaa cgactattgc gtgaggctgg catgcaaaac ctgcgtcgcg
     1501 agggagatga tctattaacc cctcatggca aactatcggt ctcgatcgcc acagtgtcac
     1561 cggtgagcgt acttattcac tgggctctaa atatcgatgg cacgggggca cccgttccta
     1621 ccgcgcaatt agcggattta gggtgggggc gagagcaatc tttaggcttt gccaaaaaag
     1681 tacttgaaca ttacattggg gaaatcgagg agattcgaat tgcccaatgc aaagtacgtc
     1741 ccgtttgatt taaaactgga tttgtccgaa catcttaaaa aactcaagcc atacgtttct
     1801 ggcaaaccca tcgaacagac caaacgtgag tttggtttga gcgaggttat taaacttgcc
     1861 agcaatgaaa accctaccgg tgcctctccg agagcgctcg aggctgtacg taaaacactt
     1921 gagcagagtc atttataccc ggatgcgagc taccagagcc tcaaaaacgc aatcgcgaag
     1981 aaacgcatgt tggatccgaa tcaggttgtt gtaggcgccg gatccaatga aatcatcgac
     2041 cttgccattc gagtattgct gcaccccggg caaaactgtg tggctccggc ctattcattc
     2101 atagcgtatc agttgtgtgc agagttaaac ggcgtcgaat ttcgcacggc taaactagat
     2161 gcggattttg ctatatcggt agatcagata ttggcggtag tggatgcgaa cactcgagtc
     2221 gtgttcttag ctaatccgaa caacccaacg ggtattacac tcgaagctaa agagctccgc
     2281 agattggcca gtggtcttct taaaaaaaat gtaattctgt gtttggatta tgcctactgg
     2341 gaatttgcgg attcaaaaga ctttcctgct gctgaagatt tgttagtgga ttacccgaat
     2401 ctgctagtgc ttcatacctt ttcaaaaatt tatggtctcg cgggatttcg agtgggttat
     2461 ggtttgggac gaccggaact aattcagtgg atcgaacgag cgcgccaacc gtttaatgtt
     2521 tcgtcgattg caattgaagc cgctattgcg gcgctagagg atcaagcctt cttaattcga
     2581 gtaaaagagc aaaatgcaaa agaacgtgaa cgtctagcta tggcgctttc gcgtcttgga
     2641 cttcgagttt acggatcgaa cgctaacttc gtgtttgtgg atttgcaccg agacgctgaa
     2701 caagtgaatt cggaattgct caaacgcggt gtgattattc gcccgctttt gaattatggt
     2761 ttgaaaaccc aggtgcgggt aactgtagga ctaccgcaag agaacgataa gttgctgaaa
     2821 gctttaaaag aggtgttggt atgagcgagt cgagattcgt ggttgcaatc gatggtcccg
     2881 ccggtagtgg caaaagcacc gtcacccatt tgctagctaa gcgtttggga tttgctcata
     2941 ttgatacggg tgcgctttac cgggccatcg cctaccgtgc gactcaaatg aaaacttcac
     3001 tcgacgatgc cttagcccta ggaaaaatag cgacctcggc gaaactttcg tttcagtggc
     3061 ttaatgatcg gaatcaaata tttttagacg gcgaaaacgt ttcgagtatg attcgcactc
     3121 ccgaaattag tatggcggcc agcaagattt ctgcctacgc gccagtgcgg gacgcgcttc
     3181 tgcagttgca acgagacctt ggggctaggc ctccgggagc agtgctagag ggtcgcgata
     3241 tcggcacagt agtttttcct aacgccgaaa ttaaatttta tctgaatgct tcggtcgata
     3301 gccgagcgag acgtcgaatg gtggaattag aggaaaaagg aacttttctt gattttgaag
     3361 aggttaaacg ccaggtagtc gaacgcgata aaggcgacat ggagagaaaa gtagcaccat
     3421 taaaaaaagc agatgatgca atcgaagtgg atacaaccaa catgaatgtc gagcaggttg
     3481 tcgacttcct gtttgcgctg gtgcaggagc gacgcggact agcccagtct ggccgttcaa
     3541 aggggcgata gcttgcgctt gctcgtggtt aggcccgatc gcataggtga cgtggttctt
     3601 tcgacccccg ccttgcagtt attaaaggcg gctattcccg atgctgaggt ccacgttctg
     3661 gtaaataaaa acgttgcgcc ggtgcttgca cagctaccct tcgtgagccg aatcatcagt
     3721 ccgccgagct ccggaagtgc gatggatcgc ctcaggaagc tgtcgcaaat gcttggcgcc
     3781 gaaagatacg atcttgcgat cgttctgcag gttgaaccac tttcgactct tgccgttcga
     3841 ctggcccgaa ttccgaaccg ggtaggacct cgatcgaagt tctattctta catggcattt
     3901 aattatgggc tcaaacaacg ccgttcggat tccaaacaaa acg
//
LOCUS       DAOMXO010000150         3908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12642_length_3908_cov_6.492084, whole genome shotgun sequence.
ACCESSION   DAOMXO010000150 DAOMXO010000000
VERSION     DAOMXO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12642_length_3908_cov_6.492084"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLH57_04250"
     CDS             complement(<1..389)
                     /locus_tag="PLH57_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01852.1"
                     /translation="MRRLFNLDETNDDQVGVCIADIANQVVEYQNSTCARICGDQKGR
                     TCANAGFTFCSRPFDFIKFGQGMHVSKSEQCCDGYFDLFLYNNTREKIICILPLTEIL
                     SARESEFNKFPLTPREREIVSLLLRRYS"
     gene            526..1098
                     /locus_tag="PLH57_04255"
     CDS             526..1098
                     /locus_tag="PLH57_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRK01853.1"
                     /translation="MQSDEALEAINGEVLEILVSNHAEFKKFLRKRMPSPAAADDLLQ
                     QCLAKAVEKKDELRDNQSVVAWFYSILRNALIDTYRARASENEKYASYLKELTTTGAD
                     RVAALDDELEAAVCACMTRLLPTLKFEYGDVLRRVDLEGASLAEVAEEFGTTVNNLTV
                     RLHRARRALKDSLVRSCGTCTEHGCLDCSC"
     gene            1181..3403
                     /locus_tag="PLH57_04260"
     CDS             1181..3403
                     /locus_tag="PLH57_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HRK01854.1"
                     /translation="MKKSCCHTGSHESKTHEKQPESCHSPSKPKKAVSQHSGVHTCPM
                     HPEVRNDGPGSCPLCGMALEPLDIGEAADAPNPELVDFTKRFGLSLFAVIPLLIIAMG
                     EMIPGNPFHEWFPGSSSNWIQMLLAAPVVLWAGAPLFHRGWYSVRTGNLNMFTLIALG
                     TGVAFGFSVFATIFPDLFPASFRGHGGRVGVYFEASAVIVALVLLGQVLELRARGRTS
                     AAILSLLKLAPNSARVIAANGEEFDVDINEVKPGDLLRVRPGEQVPVDGEVVDGHSTV
                     DESMLTGEPLPVEKLKGTNLKAGTTNQSGTLVFRATSVGSETLLSKIVRLVNEAQRSR
                     ANIQSLADAVSAWFVPAVIVSAAATAVVWAVWGPEPAYVYGLINAVAVLIIACPCALG
                     LATPMSVMVATGRGAQAGVLVRNAQAVEALEKVNTLVVDKTGTLTEGKPTLAQIRTFA
                     GFTELDVLEIAAAVERSSEHPLAAAVLAGAKKRGAIGARSVQNFKTIPGMGVSANVDG
                     KDILLGNQALLGSIAEGMADAAQGMRAEGLTVLLLAIAGQPAAIFGVGDLIKNTTPEA
                     LTALREYGLRIVMVTGDHPDTAKAVANRLGIVDVHAGVLPDGKLEIIRSLQASGQRVA
                     MAGDGINDAPALAQADVGIAMGTGTDVAMQSAGITLINGDLRGVVKALRLSRAMLRNI
                     RQNLFFAFIYNVLGVPVAAGVLFPFFGVLLSPMLASAAMSLSSVSVIANALRLRKVKL
                     "
     gene            3400..>3908
                     /locus_tag="PLH57_04265"
     CDS             3400..>3908
                     /locus_tag="PLH57_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRK01855.1"
                     /translation="MRARNLILGLGLLLSLVGSAHAKTVRYELTATRGSVDLSGRGPV
                     DFALMLNGKIPAPILEFTEGDEAEIVVRNNIPGEELSIHWHGILLPSEMDGVPYVTTP
                     PIFSGDSFTFRFKIRQHGTYWYHSHTNVQEQKGLYGAFVIHPKKKKYSTNYDRVVVLS
                     DWSDEDALT"
BASE COUNT          947 a          845 c         1118 g          998 t
ORIGIN      
        1 gaatagcggc gcaaaagaag actaacaatt tctctctccc gtggtgtgag tgggaatttg
       61 ttaaactcag attctcttgc gcttaagatt tcggtcagcg ggagaatgca aataattttt
      121 tctcgggtgt tgttatataa aaaaaggtca aagtagccgt cgcagcattg ctcgctcttg
      181 gagacatgca ttccttgacc aaactttata aaatcaaagg gtcgtgaaca aaacgtgaat
      241 ccagcgttgg cgcatgtgcg gcctttttga tcaccgcaaa tgcgcgcgca cgtggagttt
      301 tggtactcta cgacttgatt tgcaatgtcg gcaatgcaaa caccgacttg gtcatcgtta
      361 gtttcatcaa gattaaataa tctgcgcata atatttgcct taagctgcga taatagaaca
      421 aaatggtttt ctccactatc cccccaaggg gggggggctt tccaggccat gaggtgactc
      481 ggtcgctttg ttggcgtttg attgatttct aggtatgtag acaacatgca aagcgacgaa
      541 gcgcttgagg ctataaatgg cgaagttttg gaaatattag tgtcgaacca cgcggaattt
      601 aaaaaattcc tgcgcaagcg tatgccgagt ccagctgcgg cagacgattt gcttcagcag
      661 tgtctggcga aagcggtcga aaaaaaagat gaactccggg acaaccaaag cgtggtggct
      721 tggttttatt cgatattgag aaatgccttg attgatacct accgcgcacg ggcttctgag
      781 aacgaaaaat atgccagcta cttaaaagag ttgaccacca ccggcgcgga ccgagtggct
      841 gcactcgacg acgaactcga ggcagcggtt tgcgcatgca tgacaaggct tttaccgacc
      901 ttaaaattcg aatatggaga cgtgctacga cgggttgatc tcgagggggc gtcgctcgct
      961 gaagtggccg aggaattcgg tacgacggtc aataatctga ccgtgcgcct ccatcgggcg
     1021 cgtcgggcat taaaagatag tttagttcgg tcgtgcggta cctgcactga gcacggttgt
     1081 ttggactgca gctgctgacg gaaaacctaa tagtatcaag ttattagatt gtactgtaag
     1141 agattgggcg tagctccgtc tatgagtgtg gaggttcatt atgaagaagt catgttgtca
     1201 cactggttct cacgagtcca aaacacacga aaagcaaccg gagtcttgcc acagcccgag
     1261 caaacctaaa aaagctgtga gccaacactc cggtgtgcac acctgcccca tgcaccctga
     1321 agttcgaaac gatggcccag ggagctgccc actttgcggt atggcgctcg agccgttaga
     1381 tattggagag gctgccgatg caccgaatcc agagttggtg gatttcacca aacggtttgg
     1441 tttgtcgctt tttgcggtga tcccactgct catcattgca atgggtgaaa tgattcctgg
     1501 aaatcctttt catgaatggt ttccgggttc gagttcgaat tggattcaaa tgttgcttgc
     1561 ggcaccggtc gtgctttggg cgggtgcgcc gttgttccac cgaggttggt actcggtacg
     1621 taccggcaac ctaaatatgt tcacactaat tgcgctagga actggcgtag cgtttgggtt
     1681 tagtgttttc gccacgatat ttcctgatct gtttccggcc tcttttcgag ggcacggcgg
     1741 tcgcgtcgga gtgtattttg aggcttcagc cgtcatcgta gctttagtgc tattgggcca
     1801 agtgttagaa cttcgggctc gcggaagaac gagtgccgcg atactttcgt tgttaaaatt
     1861 ggctcctaat tccgcgcgag tcattgctgc taacggcgaa gaatttgacg tcgatataaa
     1921 cgaagtgaaa ccaggggatc ttttgcgcgt tcgaccagga gagcaggtac cagtagatgg
     1981 tgaggtggtc gatggccata gcacagtaga tgagtcaatg cttactggtg agcccctccc
     2041 ggtagaaaaa ctaaaaggca ccaacttgaa ggcgggaacc actaaccaaa gtggaacgct
     2101 ggtttttcgc gccactagtg tgggttctga aacgctgctt tcaaagattg tgcgattggt
     2161 gaacgaagcg cagcggagcc gagctaatat tcaaagtctt gccgacgcgg tttcggcttg
     2221 gtttgttccg gcggtaattg tgagtgcggc tgctactgcg gtcgtttggg cggtgtgggg
     2281 gccagagccc gcatatgttt acggattaat aaatgccgta gctgtattga tcatcgcgtg
     2341 tccctgcgcg cttggtttgg caacgccaat gtcggtcatg gtggcgactg gtcgtggagc
     2401 tcaggccgga gtattggtgc ggaatgcaca ggccgttgaa gcactagaaa aagtgaatac
     2461 cttggtagtc gataagacgg gcactttaac cgagggtaaa cccacgctgg cgcaaattcg
     2521 tacgttcgcg gggtttacgg aacttgatgt tttagaaata gctgctgcag tagagcgctc
     2581 tagtgagcac ccgttggcgg ctgccgtttt ggcaggcgct aaaaaaagag gggcgattgg
     2641 cgcgagatcc gtacaaaatt tcaagaccat acccgggatg ggtgttagtg caaacgttga
     2701 tggtaaagac attttactcg gaaatcaggc gctgctggga agtatagcag agggaatggc
     2761 cgatgcggca caaggtatgc gagcagaggg tctaacggtt ttgttacttg cgatcgctgg
     2821 gcaaccggcg gcgatctttg gggtgggcga tttgattaag aatacgaccc ccgaagcctt
     2881 gacggctctt cgcgaatacg gattgcggat tgtcatggta acaggtgacc atcctgatac
     2941 ggccaaagcg gtggcaaatc gtttaggcat cgtcgatgta cacgcgggcg tgttgccaga
     3001 tggcaaactt gaaatcattc gaagtttgca ggcgagtggg cagcgagtag caatggctgg
     3061 agacggcata aacgacgctc ctgctttggc tcaagctgac gttggcattg ccatgggaac
     3121 aggtaccgac gttgcgatgc aaagtgcagg aattacactg atcaacggag atctacgcgg
     3181 agtagtgaag gcgcttaggc ttagtcgtgc gatgttgcgc aatattcgcc agaatctgtt
     3241 ttttgccttt atctacaatg tgctgggagt tccagtagct gccggagttc tgtttccatt
     3301 tttcggtgta ctgctgagtc caatgttagc aagtgcagca atgagtctca gttcagtttc
     3361 agtcattgcc aatgcattac gtctgcgaaa ggtgaagtta tgagagcacg aaacttgata
     3421 ctaggcttag ggttgctgtt gagtttggta gggtcggcgc acgccaaaac agtgcgctac
     3481 gaactgactg cgactcgagg ttcggttgac ctaagtggtc gcgggccagt ggattttgca
     3541 ctcatgttaa atggaaaaat cccggctccg attctagagt tcactgaggg tgacgaggca
     3601 gagattgtgg ttcgaaataa tattccgggt gaagagcttt cgattcactg gcacggaata
     3661 ttattgccct cggaaatgga cggtgtgccg tatgtcacga cgccgccgat ttttagtgga
     3721 gattcattca cctttcgttt caaaattcgg cagcacggta cgtattggta ccactcgcat
     3781 accaatgttc aggaacaaaa agggttatac ggcgctttcg tgattcatcc gaaaaagaaa
     3841 aagtattcga cgaattatga ccgcgtcgta gttttgagtg actggtctga cgaagacgca
     3901 cttacgat
//
LOCUS       DAOMXO010000151         3896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12694_length_3896_cov_6.052070, whole genome shotgun sequence.
ACCESSION   DAOMXO010000151 DAOMXO010000000
VERSION     DAOMXO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12694_length_3896_cov_6.052070"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1388)
                     /locus_tag="PLH57_04270"
     CDS             complement(30..1388)
                     /locus_tag="PLH57_04270"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRK01856.1"
                     /translation="MPVIAAFVAGCSPSLLKKRIASVLAAASATEEVTISTFSVMLGT
                     AVNDTSCANCLAVDGSGNIYIAAMTAGSLSEANAGSTDVFVLKLDSTGSTVWTRQLGN
                     VTIGAGANNVDTVAGIRVNAAGEVFIAGQTLGSLGEANAGSGDAYVAKLTAAGAVSWI
                     KQFGNVTIGANAALNEDVTGIGLLSGGDVVVGGYTQGNLAEVRGGASDPWIARLDSST
                     GALSWITQVGSVTHASGSPATETPLGGLVVDGSDNIYLSGRTSGAFGEASAGSNDAWV
                     MKMDSGGTYTWIRQLGNVSVGVSAAGSEFAQSIAIDDLGDILLTGYTGGALDEANAGN
                     VDVFVAKLEPVAGAISWITQLGNVTVGAGASGTDVPIGSTTDSSESHYVIGYSNGNFG
                     EANGGTWDVFIAKFTAVGAYQSVKHYGLVTGGAAVTSADFGRGVYVHSSGKVRSIYAD
                     SG"
     gene            complement(1588..2280)
                     /locus_tag="PLH57_04275"
     CDS             complement(1588..2280)
                     /locus_tag="PLH57_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01857.1"
                     /translation="MLSKITAMFFMLWVYFCSTVAAQTVSVPILRGTAPPVATELPKP
                     KQRIVEKRRIPVQPTSAPLGAEGSSEQELVGELQSAREAAKAKVEQLNQLNPLVKPPE
                     QEMQTTDEAIAPGVAGSVQTAIENSPQLKARVLALVEATRSERFVRASHAIINSPNLR
                     WFYGFVIASFFIFIWIRRRVLAGTDRFLVRIFLKAGFGGLFVVALVALAFACFGQAAF
                     DVLSVLWAFSLR"
     gene            2338..2859
                     /locus_tag="PLH57_04280"
     CDS             2338..2859
                     /locus_tag="PLH57_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01858.1"
                     /translation="MKLRICQALLVLTIPLSALTFPFTANANYRSGYDMRAQLVEEAL
                     IRLAAEQVLLGDLDRESLTVSAQIEGTAPSSHREIANLEEEILQRKKRVEDLNERSTG
                     HLTLAKKRQIETELRENYGAISAAIARKITLSNNIETLQARSKELAEKIKKSQKKISR
                     LRAQISAAGDDQR"
     gene            2986..3291
                     /locus_tag="PLH57_04285"
     CDS             2986..3291
                     /locus_tag="PLH57_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01859.1"
                     /translation="MENDPERVLALQSAATNPVGAVINSLKFPIQSAKLREKKMMALF
                     FNRKLRKMLGGSRSHEKLHLAVLKIGDFEAEKPSDDCNDLLGSFALQLKKHKSDFGY"
     gene            complement(3288..>3896)
                     /locus_tag="PLH57_04290"
     CDS             complement(3288..>3896)
                     /locus_tag="PLH57_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRK01860.1"
                     /translation="GYASTVAASNPRSIFAEDLDKDGDLDLVVALRDNGEVGRFLNNG
                     TGGFGAIARTTIGDHGAGTEYPVSIRAGDFNRDGNIDVAATLETDFVGLLWGDGAGAF
                     PTQNGYDIGGAGLISHDGAVADFNFDGFLDVFVFVNNPGIGTELFYGDGTTGATGMTS
                     VGTNTGCDAGRHMVTADFDRDGAPDVAGTCDTTGEIYIYLSN"
BASE COUNT         1060 a         1101 c          859 g          876 t
ORIGIN      
        1 caggaggggt cgagaagttg ccaaaacctt caccctgaat ctgcgtaaat actcctaact
       61 tttccgcttg aatgaacgta aaccccacgg ccaaagtccg ccgaagtaac tgcagctccg
      121 ccagtcacaa gcccatagtg ttttactgac tgataagccc caactgccgt aaatttagca
      181 ataaacacgt cccaggtacc accatttgcc tcaccgaagt taccgttgga atatccaatc
      241 acgtagtgac tctccgaact atcggtagtg ctgccgatgg gcacgtcggt accgcttgcc
      301 cccgctccaa cggtcacgtt tccgagttgg gttatccaag aaattgctcc agccactggt
      361 tccaatttcg ccacaaacac atctacgtta ccggcgtttg cttcatctag ggcgccgccc
      421 gtatagcctg taagtagaat gtcaccaaga tcatcaatcg caatgctttg tgcgaactcc
      481 gaacctgcgg ccgaaacgcc gacagacacg ttccccaact ggcgtatcca agtgtatgtt
      541 ccacctgaat ccattttcat cacccacgca tcgttggaac cagcggaagc ttcgccaaag
      601 gcgccacttg ttcggccaga aagataaata ttgtcgctgc cgtcgaccac caacccaccc
      661 aaaggagttt cggttgccgg ggatccagat gcgtgggtaa cactgcccac ttgggtgatc
      721 caactaaggg cgccagtaga gctatcgaga cgagcaatcc aggggtcaga tgcaccaccg
      781 cgcacttcgg caagattccc ttgcgtgtag cctccgacga caacatcacc accagagaga
      841 agaccaatac ccgtaacgtc ttcgttgagc gcggcatttg cgccaatggt cacgttgccg
      901 aactgtttga tccaacttac agcccccgct gcagttaact tagctacata cgcatcgccg
      961 ctacccgcat tggcttcccc caatgagcca agggtttgcc ctgcaataaa cacctctccc
     1021 gcggcgttca ctcgaatacc cgcgacagtg tcgacgttgt ttgcaccggc gccgatcgtt
     1081 acgtttccga gttggcgcgt ccaaacggtt gatccggttg aatctaattt caaaacaaag
     1141 acatcggtgc ttccagcgtt cgcctcagaa agtgaaccag cagtcatcgc agcaatgtaa
     1201 atatttcccg acccatcgac cgcgagacag ttggcacagg atgtgtcgtt cactgcggta
     1261 cccaacatga ccgaaaaggt ggatattgta acttcttcag tggcgctggc agcagccaag
     1321 actgaggcga ttcgcttctt taacaacgaa ggactacacc ctgcgacgaa cgcagcaata
     1381 actggcaata agacgcagtg taacggccgg cgtttcaatc caatgataaa gcgaagacct
     1441 cccaatcatc attaaatcgg cgtaacccat tgatatgttt attttagcga attatggacc
     1501 tgggaggtac tcgattgatt acaaaattaa tgaataatta caactagttg aattaaatct
     1561 taagaaatca ttaaggtgtc gttttattca ccgcaacgaa aatgcccata aaacgctcaa
     1621 aacgtcaaaa gcagcctggc caaaacaagc aaaagccaaa gccacaagcg caacaacaaa
     1681 aagaccgcca aagcccgcct tgagaaatat tcgaactaaa aaccgatcgg ttccggccag
     1741 tactctgcgg cggatccaga taaatataaa aaatgaggca atgacgaacc cgtaaaacca
     1801 acgaaggttt gggctattga taatcgcatg ggaggctcgt acgaaacgct cactgcgagt
     1861 cgcctctacc aaggcaagca ctcgtgcctt tagctgcggg gaattttcaa tggccgtctg
     1921 cacactcccg gccactcccg gcgcgatcgc ttcgtcggtg gtctgcattt cttgctctgg
     1981 gggtttaacc aatggattca attgattgag ctgctcgacc ttcgcttttg ccgcctctcg
     2041 agcactctgc agttctccaa ccagttcttg ttcgctacta ccctcggctc ccaacggcgc
     2101 cgatgttggc tgaactggaa ttctgcgctt ttcgacgatc cgttgtttag gctttggcaa
     2161 ctccgtggca acaggcgggg ccgtgccccg cagaatcgga acactcacgg tctgagccgc
     2221 tacagtcgaa caaaaataga cccataacat gaagaacatt gccgttattt tcgataacac
     2281 ctttgcgcct tgtcaacacg attgaaaact cccgcccttt aatgctaggt gcggatagtg
     2341 aagttaagaa tttgccaagc attactcgtg ctcaccatac cgttgagcgc cctcacattc
     2401 ccttttactg ccaacgccaa ttatcgttcc ggttacgaca tgcgagctca gcttgttgaa
     2461 gaagccctca ttcgattagc agccgagcaa gttctattgg gcgaccttga tagagaaagc
     2521 ctaacagtca gtgcacaaat cgaaggaact gccccgtcga gccaccgtga aatcgccaac
     2581 ctcgaagaag aaattcttca aagaaaaaaa cgggtcgaag atctaaatga acgatccact
     2641 ggtcacctca ctcttgcaaa aaaacgccag atcgaaacag aacttcggga aaactacggc
     2701 gccatctcgg cggctattgc ccgtaagatt accctttcaa acaacattga aactctccaa
     2761 gcacgttcta aagagttggc cgaaaaaatt aaaaaatctc agaaaaagat ttctcgatta
     2821 agggctcaaa tttctgccgc cggagacgat caacggtagc gactctggcg catcttcgat
     2881 cgcacaatcg aaaaaatcga taaccgcgag acaatagaaa atctagcact tcaactggcc
     2941 gatcgaatcg cagagctgcg tggtgaactg cagcgtctgc ccgatttgga aaatgacccc
     3001 gagcgcgtgc ttgcacttca gtccgcggcg accaatccag taggagcagt gatcaattcg
     3061 ttaaagtttc cgatccaaag tgccaaattg cgcgagaaaa aaatgatggc gttgtttttc
     3121 aatcgcaaac tacgcaaaat gcttggcggt agtcgttctc atgaaaaact tcaccttgct
     3181 gtgctgaaga tcggcgattt tgaggccgaa aaacctagcg acgactgtaa tgacctactc
     3241 ggttctttcg ctcttcaatt aaaaaagcac aaatcagatt tcggctatta gttactaagg
     3301 taaatataga tttccccagt cgtatcgcag gttcccgcta cgtccggtgc accatctcgg
     3361 tcgaaatccg cagtcaccat gtggcgcccg gcatcgcacc ctgtattggt tccaaccgaa
     3421 gtcatgccgg tggccccggt cgtcccgtcg ccataaaata gctcggtgcc aatacccgga
     3481 ttatttacga atacaaacac atcgagaaaa ccgtcgaagt taaaatcggc cactgccccg
     3541 tcgtgtgaaa tcaatccagc gccaccaata tcgtagccat tttgggtcgg aaaagccccg
     3601 gccccgtcgc cccacaataa tcctacgaaa tcagtctcaa gcgtcgcagc aacgtcaatg
     3661 tttccgtctc gattgaaatc ccccgctcgg atactaacgg ggtattcagt tcccgcgcca
     3721 tggtcaccaa tggtcgtgcg ggcaatagca ccaaatccac cagttccgtt gttcaagaat
     3781 cttccaactt ccccattgtc gcgcaacgct acgaccaaat ccaaatcacc gtctttgtcc
     3841 agatcttcag caaatatgct tctcggatta ctagctgcaa ccgtcgaagc ataacc
//
LOCUS       DAOMXO010000152         3892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12720_length_3892_cov_3.846234, whole genome shotgun sequence.
ACCESSION   DAOMXO010000152 DAOMXO010000000
VERSION     DAOMXO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12720_length_3892_cov_3.846234"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLH57_04295"
     CDS             complement(<1..588)
                     /locus_tag="PLH57_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01861.1"
                     /translation="MNSQIGFFILVVFAILSSVDTVGAVDTELGCNVIAAIAEERAKS
                     KTTRKYNTSENASIEVSTPDSALDKTTADTISETIAGVNLLLDDHLAIPAEMKVIVGR
                     GLKDGYNWASDSVSLNSEASLKVGNRTFRKHPKHRVTTTAHEYTHSIFDLNTRELLKT
                     EHLHRARELGDRNAIVQEIRNLEQRIVNLETTIKSG"
     gene            complement(647..1237)
                     /locus_tag="PLH57_04300"
     CDS             complement(647..1237)
                     /locus_tag="PLH57_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRK01862.1"
                     /translation="MNFSAMKKIVVLSALALIFGATDASAQSKKKNTNTRPVYKSVDA
                     SNSDSDAFKGRPRTNEHELGAGLGLNYFSDFGFQARYAYRAMPEGFIEGVNDAFLIEG
                     GVGMTFYGRVKSENVTGVHFFAAGRWDFTKDELWTFFGTLGLGYNATSIDTGDVKGGG
                     IFPVVGVGALYEINSDWAVRADFSYHFLGLGMAYRF"
     gene            complement(1278..1859)
                     /locus_tag="PLH57_04305"
     CDS             complement(1278..1859)
                     /locus_tag="PLH57_04305"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRK01863.1"
                     /translation="MTGSNQQNPTKAAVLVPIFVTQNPQSALAPLWEIPGTGKSVPLN
                     EHTNVLFTLRTHSVEHHKGQISFPGGVYEPSDRDLLATALRETHEEVGLAPDQVQVLG
                     PLPKAITVTSAFEIHPFVGIIEANSVQLQINPYEIETSIFVPLPVLLDSASQKIETVE
                     HEGRQYKLRSYFYREHRIWGATARILSSLLSHT"
     gene            1883..2881
                     /gene="epmA"
                     /locus_tag="PLH57_04310"
     CDS             1883..2881
                     /gene="epmA"
                     /locus_tag="PLH57_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HRK01864.1"
                     /translation="MQRSKDVLEKRAQIIRTIREFFWSQGFIETDTPTLVVSPGMEPH
                     IRPIATENPKVFLPTSPEFAMKKLLAEGFEKIFQISKAYRNEPKSATHNPEFAMLEWY
                     RTDAGYEAIMDDVEALFKTIATRHAPERYDHGSPWPRMSVREVFAQFVGLDLGQALED
                     IEMLARKCVELNLAPKDVLQKSSLNAPGAWDDFFFRIMLNVVEPRLEQMNRPIIMHSY
                     PPSQCALANMECDSEGRPWAKRFEVYAGGHELGNAFDELIDSNEQRRRFEKDMNLRKQ
                     IYGNSFKENPIDEEFLSALKKMPKSGGIAMGVDRIVMYFLEKQSIEEVLFGNSNWS"
     gene            complement(2878..3819)
                     /locus_tag="PLH57_04315"
     CDS             complement(2878..3819)
                     /locus_tag="PLH57_04315"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRK01865.1"
                     /translation="MSIQKISLKLPPDLKGRLDQSVFLAAETQAPKATLSKSFIRKLI
                     VAGGVYLNGKRCRVASRAVRGGERVDLYINSELASSSVARPPEKTLPALRVLYEDEAV
                     ICFDKPAGLPTQPTVDDARANLFEMAKKLVQKKDGPGAYLGLHHRLDRDTSGVMLLTR
                     DSKFNTFIADQFKEHLCTKIYVAVLEGSLSKQSDRIETFMAEVGRVGKIAKFGNVKSG
                     GKKAITEYTVLQRNSRFSLVELKILTGRTHQIRVHMSSLGHPVVGDFLYGSKPEYCKK
                     AGRHFLHAHSLEFTHPVTKNQILVESPVPPIFREWVD"
BASE COUNT         1011 a          873 c          947 g         1061 t
ORIGIN      
        1 gccagatttg attgtggtct cgagatttac gatccgttgt tcgagatttc gaatttcttg
       61 gacaatggca tttcggtccc ccagctctct cgccctatgc aggtgttcag ttttgagtag
      121 ctcgcgggta ttaaggtcaa aaatagaatg ggtatactcg tgagcagtgg tggtgacgcg
      181 gtgtttgggg tgcttacgaa aggtgcgatt gccaaccttg agagatgcct ctgagtttag
      241 gctcacactg tccgacgccc aattgtaacc atcttttagc cctctcccga caatgacctt
      301 catttctgcc ggaatggcga ggtgatcgtc taacaaaaga ttcacacccg cgattgtttc
      361 tgaaattgtg tcggcggttg ttttgtctaa tgcggaatca ggagtagaga cctcaattga
      421 agcattttcc gaggtgttgt attttcgtgt tgttttgctt ttagctcttt cttcggcaat
      481 cgcggcaatg acattgcagc caagttcggt gtctaccgcc ccgacggtat caaccgaact
      541 taatatcgcg aagacaacta aaataaaaaa accgatctga gagttcacca ctcattagat
      601 cggtttttat taactatcta ttcaatgcgg caaaaattgc cgagacttaa aatctgtagg
      661 ccatgccgag accaaggaag tggtacgaaa aatccgctct aacagcccaa tcggaattga
      721 tttcgtacaa cgctcccacg ccaactactg gaaaaattcc accgccctta acatcgccag
      781 tgtcgatgct ggtcgcgttg tagcccagtc ctaacgtacc aaaaaaggtc cagagttcgt
      841 ctttagtgaa gtcccagcgc ccggccgcga aaaagtgtac acccgttacg ttttccgatt
      901 ttactcggcc gtaaaaagtc attccaactc cgccttcgat taaaaacgcg tcgtttacac
      961 cttcgatgaa gccctcgggc atcgcccgat atgcatagcg agcttgaaag ccgaagtcgc
     1021 taaagtagtt tagtccgagg cctgccccaa gctcgtgttc gttggtacgc gggcgtcctt
     1081 taaaggcgtc gctatcggaa ttagatgcgt cgactgattt ataaaccggg cgggtattgg
     1141 tatttttttt ctttgattgt gcactagcat ctgtcgcacc aaagatgagt gcgagcgcgc
     1201 ttaaaacgac gatttttttc atagctgaaa aattcataaa gcctccgttc gttagattga
     1261 gtgccgagtt taaaggctca cgtatgcgaa agcaaggacg agagtattct cgcggtggcg
     1321 ccccagattc gatgttctct ataaaaataa gatcgtaact tgtattgacg cccctcgtgt
     1381 tcgacggttt caattttttg actggccgaa tctaatagta cgggcagcgg cacgaagatt
     1441 gaggtttcta tttcataggg gttgatttga agctgtacac tattcgcttc gataattcct
     1501 acaaacgggt gaatttcaaa agccgaggtt acggtaatcg ctttcggtag tggacccaaa
     1561 acctgtacct gatctggcgc aagccctact tcctcgtggg tctcgcgcag tgccgttgcc
     1621 agaaggtcgc gatcacttgg ttcgtaaacc cctccaggaa aactaatttg gcctttgtga
     1681 tgttctacgc tgtgggtacg cagtgtaaaa agcacattcg tgtgttcgtt gagcggcacg
     1741 cttttaccgg tgccaggaat ttcccaaagt ggagccaggg cactttgagg attttgggta
     1801 acaaaaatgg gcaccaggac ggccgctttg gttgggtttt gttggttgct cccagtcata
     1861 attctggatt atacgtagcg cgatgcaacg aagtaaagat gtactggaaa aacgggctca
     1921 aatcattcgc acgattcgcg agtttttttg gtcacaggga tttatagaaa ccgatacccc
     1981 taccttggtt gtttcgcctg ggatggaacc gcatatccgg cccattgcca ccgaaaatcc
     2041 caaggtattc cttccaacta gtccggaatt cgcgatgaag aagttattgg ccgaaggctt
     2101 tgaaaaaata tttcaaattt caaaggctta tcgcaacgaa cctaaaagtg cgactcataa
     2161 cccagagttt gcgatgttgg aatggtatcg cactgacgcg ggttacgagg cgatcatgga
     2221 tgacgttgag gcactgttta aaacgatcgc aactcgccat gctcccgagc gatatgatca
     2281 tgggtcgcca tggccacgca tgtcggttcg agaagttttt gcgcagtttg tgggcctcga
     2341 tctcggacaa gctttagaag atatcgaaat gcttgcccga aaatgcgtag aattaaatct
     2401 cgcaccaaaa gacgtgcttc aaaaatcgag tttaaatgca cccggggcat gggacgattt
     2461 tttctttcgt ataatgttga atgtagtgga gccgcgactt gagcaaatga atcggccgat
     2521 tatcatgcac tcctacccac ctagccaatg tgcacttgct aatatggaat gtgattctga
     2581 gggtcgaccc tgggctaagc gttttgaagt ttatgcaggc ggacatgaac tcggcaatgc
     2641 tttcgacgag ttaatcgatt caaatgaaca acgtcgtcgt ttcgaaaaag acatgaattt
     2701 gcgaaagcag atttacggta attcatttaa ggaaaatcca atcgatgaag agttcctgag
     2761 tgcgcttaaa aaaatgccaa aaagcggtgg aattgccatg ggcgtggatc gaatcgtcat
     2821 gtatttcttg gaaaaacaat cgatcgagga agtgcttttc ggtaattcta actggagcta
     2881 atcaacccat tcccgaaaaa tcggtggaac agggctttcc actaggatct gatttttggt
     2941 caccggatga gtaaattcaa gcgagtgtgc gtgtaaaaag tggcggcctg cttttttgca
     3001 gtattcaggt ttggaaccat atagaaaatc cccaactacc gggtgtccaa gagagctcat
     3061 gtgaacgcga atttggtggg tgcgacctgt aagaattttg agttcaacca gagaaaaacg
     3121 agaatttcgt tggagaacag tgtattcggt gatggctttt tttcctccag attttacgtt
     3181 tccgaattta gctatttttc ctactcgccc gacttcggcc ataaaggtct cgattcgatc
     3241 agactgtttt gagagcgatc cctcgagcac tgcgacataa attttggtac aaaggtgctc
     3301 tttaaactga tcggcaatga atgtgttgaa tttcgaatcg cgtgtaagga gcatgacccc
     3361 tgaagtatcg cgatccaaac ggtgatgaag cccaagatag gcaccgggtc catctttttt
     3421 ttgaactaat tttttggcca tttcaaaaag attagcgcga gcgtcgtcga cggttggttg
     3481 agtcggcagt cccgcaggtt tgtcaaagca gatcaccgct tcgtcttcat agagcactcg
     3541 cagtgcggga agtgttttct ccggtggacg agcgacagaa ctagatgcaa gttcgctgtt
     3601 aatgtaaaga tcaactcgct cgccgccccg cacagctcga gacgcgacgc ggcaacgctt
     3661 tccgtttaaa taaacaccac ccgcgacaat caattttcgt atgaacgact tagaaagggt
     3721 cgctttaggc gcctgggtct cggcagcgag aaatacactc tggtcaagtc gacccttgag
     3781 atcaggcgga agttttaaag aaattttttg gatactcata atatttcttt ccacctatgc
     3841 ataaatgaat tgaaacccgc tttgcaaaaa aaatatactg aactcgtctc ta
//
LOCUS       DAOMXO010000153         3890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12728_length_3890_cov_4.477966, whole genome shotgun sequence.
ACCESSION   DAOMXO010000153 DAOMXO010000000
VERSION     DAOMXO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12728_length_3890_cov_4.477966"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLH57_04320"
     CDS             complement(<1..654)
                     /locus_tag="PLH57_04320"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01866.1"
                     /translation="MFELSLKCRCSTVAHSQSALPRSSKFYLPEAKAHYSPDRLIDVE
                     HIKLDIAVDLKKKWLGGTCLTRFIVQTPTVAKLKFDAVDLKVSKVTDPDGKTLEFNQS
                     SDSIFVLLNRELKCGERSEVTFTYEVTEPRAGLYFVGPNEFDSKRPNQLWTQGEDQDN
                     RYWIPCHDAPNEKATTEMLVTVEEHLTVISNGGLVAVKHDSARKKKTFHWSQKIPHAS
                     "
     gene            complement(656..1759)
                     /gene="mutY"
                     /locus_tag="PLH57_04325"
     CDS             complement(656..1759)
                     /gene="mutY"
                     /locus_tag="PLH57_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRK01867.1"
                     /translation="MLQIDRKSIASLVEWFRKNQRDLPWRRDPHPYKVWVAEVMSQQT
                     QMNTLLPYFHRFMERFPSVGHLAQSSIEEVFVAWTGLGYYSRARNLHRAAKTVTEDLK
                     GRFPTSFGDWLELSGVGPYTAAAVTSQCFDSPHAVWDGNVLRVASRFFAESQAYSQGF
                     KTAVSRALEKHMSRVPSSQLNQAVMELGALVCTPVKPDCDNCPLRRKCRAFADKNVQL
                     YPPAKIKRREHVVHAVVSVHLKKTADDCFVLLKKRDSGLWYSGMWDFPTELGGVQNPQ
                     ITLLRLKDKKNDLGTIALKPIKHSITHHRISLLPRVKVEVGLNQPTEDSEVKWFNVEQ
                     AFGDRPQIPVATTARKVMRAVSRWLDQVKEKEN"
     gene            1807..2253
                     /gene="dtd"
                     /locus_tag="PLH57_04330"
     CDS             1807..2253
                     /gene="dtd"
                     /locus_tag="PLH57_04330"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRK01868.1"
                     /translation="MKCVIQRVKHASVTVQDRKIAGIDSGVLTLLGIEKGDTELELKK
                     MIQKIVELRIFEDEHGKMNRSLIDVAGAHMIISQFTLAADCTSGRRPSFTTAEVPEAA
                     RALYDKALQVSEGFGVRTCGGAFGADMKVELLNDGPVTFILDSKLI"
     gene            complement(2250..2876)
                     /locus_tag="PLH57_04335"
     CDS             complement(2250..2876)
                     /locus_tag="PLH57_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01869.1"
                     /translation="MMKMGMFLFGLVILELVGTKFAYSEKLYQPPECADTQGKRTLAV
                     DITNDLSYMATPAPKTYKNTVNEKFRKVTASKIKKEEMLCLVETECPKTEKSEDKCYL
                     LKGHKTSKSDVANIFQLKPKKEKSSSRTLERVKKASKCKDVVLCGIETIAMPLGRKAR
                     SAIITHESFQGTTSFSVGASWSDEGDDYPPNTGSAEAVLDAGESRPLD"
     gene            2979..>3890
                     /locus_tag="PLH57_04340"
     CDS             2979..>3890
                     /locus_tag="PLH57_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01870.1"
                     /translation="MKTNFLLPLVLLAATSCTSVKFAKAPDSLESGRAPANFQDDSLS
                     NKSLTTIPEDCILADSRYISKNSTKHDLYYANPNKWSYAAENDTDIKPRIVVIKAACE
                     AALQKNVTSISEKYFLTGTQLPFLPYAASSKRPYCGLSPSEDVCTAPTVAHPSYIFEV
                     DQKSFPQDVENKVKSLSENIATGKKIDLWNLEDNITIVAVEFKRSPKSNFVMHFMDKA
                     TNVLTARGDVRFIILNKRESQPKADGPQGSQFQGDDWDVVYSYYTSKIDLTKSPDFSR
                     FLKAHARSWDQDHRVEKWKIFYEGDYGN"
BASE COUNT         1026 a          949 c          867 g         1048 t
ORIGIN      
        1 cgaggcgtgc ggaatttttt ggctccaatg aaatgttttc tttttgcgag ctgaatcatg
       61 ttttaccgca actaggccac cattagaaat aacagtaaga tgctcttcca ccgtaacgag
      121 catttcggtg gtggcctttt cgttgggggc atcgtgacac ggaatccaat accgattgtc
      181 ctgatcttcc ccttgagtcc agagttggtt agggcgtttt gaatcgaatt cattgggacc
      241 cacaaagtaa agcccagcgc gaggttccgt tacctcatag gtaaaagtaa cttccgatct
      301 ttcgccacat ttaagttcac gattcaaaag aacaaatatt gaatcgctac tttgattaaa
      361 ttctaatgtt tttccatccg gatcagtgac tttactgact tttaaatcca ccgcgtcgaa
      421 cttcaacttc gcgactgtgg gagtttgaac aataaaacgt gtcaaacaag ttcctcccag
      481 ccactttttt ttaagatcaa ctgcgatgtc gagttttatg tgctctacgt cgatcaaacg
      541 atcggggctg taatgagctt tggcttcggg taaatagaat tttgagctcc ggggcaaggc
      601 gctttggctg tgagcgacag tactgcagcg acatttcaat gatagttcga acattttaat
      661 tttccttttc tttaacttgg tccaaccaac gactcacggc ccgcatgact ttacgcgcgg
      721 tggtggcaac tggtatttga ggtcgatcac caaaagcttg ctctacatta aaccatttca
      781 cctcagagtc ttccgttggc tgattcaatc cgacttcaac tttcactcga ggaagaagcg
      841 atattcgatg atgagtgatg ctgtgtttga tcggcttcag ggcgatcgtc ccgaggtcat
      901 ttttcttatc ttttaatctt aataaagtga tttgtggatt ctgaactccg cctaattctg
      961 taggaaagtc ccacattcca gaataccaaa gcccggagtc tctttttttt agcagaacaa
     1021 aacaatcgtc tgcagttttt ttcaaatgca cactcaccac ggcatgcact acgtgttccc
     1081 gccgctttat tttcgcaggt ggatacaact ggacattttt gtcagcaaaa gctctacact
     1141 tgcgacgaag cggacagttg tcgcagtctg gtttcacagg agtacaaaca agggctccaa
     1201 gctccattac agcttgattg agctgcgacg atggcacgcg agacatgtgc ttttctaaag
     1261 cccgggacac tgcggtcttg aagccttggc tgtaagcctg agattccgca aaaaatcgcg
     1321 atgcgacgcg caaaacgttg ccatcccaca ctgcgtgcgg cgaatcaaaa cactgcgaag
     1381 ttaccgcagc ggcagtgtag ggccctaccc cactgagttc caaccaatcg ccgaatgacg
     1441 tgggaaagcg accttttaga tcttccgtca cggttttagc tgctcggtgc agattccttg
     1501 cgcggctata gtaaccgaga ccagtccatg ccacgaacac ctcttcgatc gaactttgcg
     1561 ccaagtggcc aacagaaggg aaccgttcca taaagcggtg gaaataggga agaagcgtat
     1621 tcatctgagt ttgttgactc atgacttccg caacccagac tttgtagggg tgaggatctc
     1681 gacgccacgg aagatcgcgt tgatttttgc gaaaccattc tacgagcgag gcgatcgact
     1741 ttcgatctat ttgcagcata cgctctacct agcctaaatg tgtcgcttcg agtagtgttg
     1801 ggtacgatga aatgtgtaat acaacgcgta aaacacgcaa gcgtcactgt tcaggaccga
     1861 aagatagcgg gcatcgattc tggggtgctc acgctgttgg gtatcgaaaa aggcgatacc
     1921 gaactcgaat taaaaaagat gattcaaaaa attgtggaac tccgaatatt tgaagacgag
     1981 catggcaaaa tgaaccgttc gctcatagac gttgcaggcg cccacatgat tatttcgcaa
     2041 tttacgttgg ctgccgactg cacgagcggg cggcgcccca gctttaccac tgcagaagta
     2101 cccgaggctg cacgagcgct atacgataaa gcgcttcaag tttcggaagg tttcggtgtt
     2161 cgcacctgcg gcggtgcttt cggcgcagac atgaaggtcg aacttctaaa cgacggtcct
     2221 gtgaccttta ttttagattc aaaattaatt tagtctaatg gtctagattc gcctgcatca
     2281 agtaccgcct cggcacttcc ggtattcggc gggtagtcat cgccttcatc actccagctt
     2341 gcgcctacgc tgaagctcgt ggtcccctgg aatgattcat gggtaattat ggcactccgt
     2401 gctttccgtc caagtggcat agcgatagtt tcaattccgc aaagtacaac gtccttacac
     2461 ttagatgctt tcttcactcg ttctagagtt ctggaagagc ttttttcctt tttgggcttt
     2521 aattgaaaga tattcgcaac atcacttttg gacgtcttgt ggcccttcaa aagatagcac
     2581 ttatcttcag acttctcggt cttgggacat tccgtttcaa cgagacacaa catttcttcc
     2641 ttcttgatct tggaagctgt aacttttctg aatttttcat taacggtatt tttataggtc
     2701 ttaggggcgg gcgtcgccat ataggaaaga tcgttggtga tatctaccgc caatgtgcgt
     2761 tttccttggg tgtctgcgca ctcagggggc tggtataatt tttcagaata cgcgaacttc
     2821 gtgccaacca gctccaggat caccaggcca aataaaaaca ttcccatttt catcacaatt
     2881 caccccttcg attcaagaat atcacgcggc atcatttgaa cactcggcaa attttcccta
     2941 ctacgattgg atgctcaaaa aaccgacaaa tatagaggat gaaaacgaat tttttattac
     3001 cactggttct acttgcagca acaagctgta cctcggtgaa atttgcgaaa gctcccgact
     3061 cattggaatc ggggcgtgcg cccgcgaatt ttcaagacga ctctctcagc aacaagtctc
     3121 tcactacgat accggaggac tgcatccttg cggatagtcg ctatatcagt aaaaactcga
     3181 ccaaacacga tctttactat gccaacccaa ataagtggtc ctacgcggcc gaaaatgaca
     3241 ccgatatcaa accacgcatt gttgtaataa aagcggcctg tgaggccgcg cttcaaaaaa
     3301 acgtgaccag tatctccgag aaatattttc tcactggaac ccaactaccc tttctccctt
     3361 atgcggcgtc ttcaaaaaga ccctactgcg gactgagtcc aagcgaagac gtttgtactg
     3421 ctccgaccgt agctcatcct agctatattt ttgaggtaga ccaaaaatca tttccccaag
     3481 acgtggaaaa caaagtcaaa tcactttcgg aaaacattgc aaccggaaaa aaaatcgatc
     3541 tatggaacct cgaagacaac atcaccatag tcgcggttga gtttaaacgt tcgccaaaat
     3601 ccaattttgt catgcacttt atggacaaag ccaccaatgt cttaaccgcc agaggagacg
     3661 tacgatttat aattctcaac aagcgggaat ctcaacccaa ggccgacgga ccgcaagggt
     3721 cgcagttcca aggtgacgat tgggatgtgg tttatagcta ctataccagc aagatcgatc
     3781 tcacgaagtc tcccgatttt tcgcgttttc tcaaagctca cgcgcgctct tgggatcaag
     3841 accaccgagt agagaaatgg aaaatttttt atgaaggtga ctatggcaat
//
LOCUS       DAOMXO010000154         3888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12738_length_3888_cov_5.397861, whole genome shotgun sequence.
ACCESSION   DAOMXO010000154 DAOMXO010000000
VERSION     DAOMXO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12738_length_3888_cov_5.397861"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLH57_04345"
     CDS             <1..294
                     /locus_tag="PLH57_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01871.1"
                     /translation="TTAYFSHAAAFLDEMRTESPANWGKYSNKNYDRIVDSIKKSNFK
                     RELIAQADRTLVHDDCSVIPLYFRPTALLLKPRWINFELNPLARVQLKKVRSR"
     gene            394..840
                     /locus_tag="PLH57_04350"
     CDS             394..840
                     /locus_tag="PLH57_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01872.1"
                     /translation="MGLRFTWVVTTLSLSLLSFAFGSEGFVELDSKFLHVNPRVIRAK
                     QDRPHAVWAKMHTRDIKRVSAILKQALADQKDISVEIESFYRETGEVRLIIRNQSRES
                     KESATLRIEEALKKNKNISLVGRRRIFRITEMRNEEHRKNSSKVAE"
     gene            837..2369
                     /locus_tag="PLH57_04355"
     CDS             837..2369
                     /locus_tag="PLH57_04355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRK01873.1"
                     /translation="MMGFPIKAKASLVICCTFAVLSAACSSQERVEKLSSGPSFRNKL
                     PPAYYTQSTLDIPANLNDRYFADKGAWSQVYTDLWAHLEIGAAALYSGITLPSNEIVV
                     AVLDTGVDTDHEDLVGRMWVNPREIPSDRYNNSVDDDQNGFVDDFVGWDWVNNTNNPS
                     DDNGHGTHVAGTIAAIGGNGKGIVGVAPFVRIMPLKVCSYDGACESSDIRDAILYANR
                     NGAKVINLSLGGPDDGPEGQAFNSVIEQVSKAGTLVVTSAGNDAMDVGEYSPANSTYA
                     MPVAAHRSDGRICGFSNFGWKLDVSAGGCGEKNGQDVGAMISANSKKCGSLGDQYCAV
                     RTAIGEKYSLKYGTSMSTPHISGLAALVWSLNPQATPLQIRQTVLKTAVKANADNKNV
                     DFGFGKASAVDAARIALEAPGIKITSPFHGAISNQHLLKLRIEAQSPVAWELRAVSVQ
                     DGDANLQLTAGDLIATGTVSAATNVDLSELYLPAQAGSYLIVLKAQVSGESYFDTALV
                     RR"
     gene            complement(2366..3496)
                     /locus_tag="PLH57_04360"
     CDS             complement(2366..3496)
                     /locus_tag="PLH57_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01874.1"
                     /translation="MGLKRFTPIFFLTLFGCSFKPLPEGLSNKLTIFLKDNTQTAALT
                     SLTRPPFAIQAPTSTTDFDCFAVNITGDGVLPDSEQFQGCSINGGAGVQSFGFFSEPF
                     KRSSSISVEIPAGDARRIDVYGIYPSTGILECGGSMGGNGDEAAGYYVGSSTINLTAS
                     TSVSIPISFASGTTAALDCEGDGHAITYTAQVNGSNAAGRYWNSCGDAALVDSTDIPP
                     ITSTAFAGAENTAVSSNDATSFNAECDYVGINFNVTVNEYAVYADLTTIQTYYDRLTI
                     YWTGSAGIYSTPCDSSSSPTGAGSGATPRMEVYNDSTSTWESITTVGTTETSITHTIT
                     NPANYYDPATNAIWFRVVGGVTNGASTCSVVKTNQVYFTFDG"
     gene            complement(3487..>3888)
                     /locus_tag="PLH57_04365"
     CDS             complement(3487..>3888)
                     /locus_tag="PLH57_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK01875.1"
                     /translation="NFSAYTASKGGVWRLTETVAAELAPFGIFVNAIAPGNVNTKFLD
                     DLLAAGPANVGKDLYERSIEQKSKGGNSPFKAADLSSYLISDASVGLYGKIISALWDD
                     YRKFENLEAMSKSDIFTVKRIITSTGSTRWD"
BASE COUNT         1057 a          901 c          947 g          983 t
ORIGIN      
        1 accacggcct attttagtca tgcagcggct tttttagatg agatgcggac agaaagcccc
       61 gccaactggg gcaaatattc taataagaat tacgatcgaa ttgtcgatag cattaaaaaa
      121 tctaacttta aaagagaact aattgctcaa gccgatcgaa cgctcgtgca tgacgactgc
      181 tcggtaattc cgctttactt tcgaccaact gctcttcttt taaaaccaag atggataaac
      241 tttgaactaa acccactagc tcgagtgcaa ctcaaaaaag ttcgatcccg ataaattcca
      301 ttggtaggca ataaattcag tatccctcgg taggtcgtta ctaagctaaa cctgccgatc
      361 gcaggaaagc ccttaatagg tcattctaag agaatggggc ttcgattcac atgggtcgtc
      421 acgactctat cgttgtcgtt actttccttt gctttcggat ctgaaggatt cgtagaactg
      481 gattcaaaat ttttgcacgt taacccacgc gtgattcgag cgaaacaaga ccgtccacat
      541 gcggtctggg cgaaaatgca cactcgtgat ataaagcggg tttccgccat tctaaagcaa
      601 gctttggctg atcaaaaaga tatatcggta gaaatagaaa gcttctaccg agaaactggt
      661 gaagtaaggt taattattcg taatcagtct cgagagtcca aagaatccgc cacattacga
      721 attgaagagg cgcttaagaa aaataaaaac atttcactgg tggggcgtcg gcgaattttt
      781 cgaattaccg agatgcgaaa tgaggaacac agaaaaaatt cctcgaaggt cgctgaatga
      841 tgggctttcc aatcaaagcg aaagcttctt tagtgatttg ctgcactttc gcagtcttat
      901 cggccgcctg cagttcgcag gagcgcgttg aaaagttatc gtcaggcccg agtttccgca
      961 acaaactacc accagcgtat tacactcaaa gcactcttga tattcctgct aatttaaatg
     1021 accgctactt cgccgacaaa ggtgcttggt cgcaggtcta caccgacttg tgggcccacc
     1081 tcgaaatagg cgctgccgcc ctttattctg gtattactct tccgtcgaac gaaatagtgg
     1141 ttgcggtact cgataccggg gtagatacgg atcacgaaga tctagtgggt cgaatgtggg
     1201 taaacccacg ggaaataccg agtgaccgat ataataactc agtggacgac gaccaaaacg
     1261 gcttcgttga cgactttgtt ggttgggact gggtgaataa taccaataat ccatccgacg
     1321 acaacggaca cgggacccat gttgcaggca cgatcgccgc aattggcggc aatggtaagg
     1381 gaattgtcgg agtagctccg ttcgttcgaa taatgccact gaaagtgtgc agctacgacg
     1441 gagcatgtga aagtagcgat attcgtgacg caattctata cgccaatcgc aacggagcta
     1501 aagtaattaa tctgagtttg ggtggtcccg acgatggacc agaaggccaa gcttttaatt
     1561 ctgttattga gcaagtgtca aaagccggca ccttggtggt taccagtgcg ggtaacgatg
     1621 cgatggacgt tggggagtat tcacccgcga attcgactta tgctatgcca gttgcggctc
     1681 atcgaagcga tgggcgtata tgtgggttct caaattttgg ttggaagctt gacgtgtctg
     1741 ccggtggttg cggcgagaaa aatggtcaag acgttggggc aatgatttcg gcgaactcca
     1801 aaaaatgtgg cagtcttgga gatcagtatt gtgcggttcg aaccgcgatc ggtgaaaagt
     1861 attcgttaaa atacggtaca tcaatgtcta cgccgcatat cagcggacta gccgcattgg
     1921 tctggtccct taatcctcaa gctaccccgc ttcagattag acagacagtt ttaaaaacag
     1981 cggtgaaagc gaatgccgat aacaaaaatg tagactttgg tttcggaaag gccagcgcgg
     2041 ttgatgctgc gagaatagca ctcgaagccc caggtattaa aataacctcg cctttccatg
     2101 gagccatttc aaatcaacat ctattgaagt taagaattga agcccagtcc cccgtggcgt
     2161 gggaacttcg ggcagtctcg gtgcaagatg gcgatgctaa tttgcaatta accgcgggtg
     2221 accttatagc tacgggcacc gtatctgctg cgaccaacgt ggacctgagt gagctttatc
     2281 tacccgcgca agcgggctct tatttgatag tactgaaagc gcaagtttca ggtgaatcgt
     2341 atttcgacac agcactcgtt cggcgttagc cgtcaaaagt aaaataaacc tgatttgttt
     2401 ttaccactga acacgtagat gccccgttcg tcaccccgcc taccacacgg aaccatatgg
     2461 cgttggtcgc agggtcgtag taattcgctg gattcgttat cgtgtgggta atcgaagttt
     2521 ccgttgttcc cacggtggtg atggattccc aagtagacgt cgaatcgttg taaacttcca
     2581 ttcgaggagt tgctccggaa ccggcaccag ttggcgagct tgaagagtcg caaggtgtgc
     2641 tgtaaatgcc cgcagagcct gtccagtata tcgttagccg atcatagtat gtctgaatgg
     2701 tcgtgaggtc cgcatacact gcgtattcgt tcactgtaac gttaaaattt atgccaacat
     2761 aatcgcattc cgcattaaaa cttgtagcat cgttggaaga aactgcagtg ttttccgcgc
     2821 ccgcaaaggc agtgctggtg ataggtggta tatcggtact atccactaac gccgcatcac
     2881 cacaagagtt ccaataacgc ccagccgcat tcgagccgtt gacctgcgcc gtgtaggtga
     2941 ttgcatgacc gtcgccctcg cagtctaggg ctgccgttgt accgctggca aagctgatgg
     3001 gaatcgaaac cgaagtagag gcggttaaat tgatggtaga gctgcccacg taatagccgg
     3061 cagcctcatc tccattgcca cccatcgacc caccacattc caaaattccg gtagaagggt
     3121 aaattccgta aacatcaatt cgccgtgcat cgccagcggg aatttccacc gaaattgagc
     3181 ttgatcgctt aaacggctca gaaaaaaaac caaaactttg aactccggcg ccaccgttta
     3241 ttgagcagcc ttgaaactgt tcactatctg gcaaaacacc gtcgccggta atgttcacag
     3301 caaaacagtc aaagtctgta gtcgaagtag gcgcttgaat cgcaaacggt ggccgggtca
     3361 gtgaagtgag agctgccgtt tgggtattgt cttttaaaaa aattgttaac ttgttggaaa
     3421 gcccctctgg aagcggctta aaggaacatc cgaataatgt gagaaaaaaa attggagtaa
     3481 atctttttag tcccatcttg ttgatcccgt cgaagtaata atgcgtttga cggtaaagat
     3541 atctgatttg ctcatggctt ccaagttttc aaatttacga taatcatccc atagggcgct
     3601 aattattttt ccgtacaatc ctaccgaggc gtcggaaatt aaataagaac taagatccgc
     3661 cgctttaaac ggagaatttc cacccttcga tttttgttca atagaccttt cgtaaagatc
     3721 ttttccaacg ttggctggcc cagctgccag taggtcgtct aaaaattttg tatttacgtt
     3781 gccgggagca atcgcgttga cgaatattcc aaagggcgcc aactcagcgg cgacggtttc
     3841 agtgagcctc cagacgcccc ccttcgacgc cgtgtaggcc gaaaaatt
//
LOCUS       DAOMXO010000155         3883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12759_length_3883_cov_4.592738, whole genome shotgun sequence.
ACCESSION   DAOMXO010000155 DAOMXO010000000
VERSION     DAOMXO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12759_length_3883_cov_4.592738"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLH57_04370"
     CDS             complement(<1..231)
                     /locus_tag="PLH57_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRK01876.1"
                     /translation="MLRETSEKIQFIDLKAQYALVGSRIDESIRRVLQHGQYILGPEV
                     VELEAKLAQYVGARHCITVASGTDGLVMALMAL"
     gene            complement(212..784)
                     /locus_tag="PLH57_04375"
     CDS             complement(212..784)
                     /locus_tag="PLH57_04375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRK01877.1"
                     /translation="MKWKNVTIHKTADVSEKAEIGEGTRVWNNCQIRESANIGRNCIL
                     SKDVYVDHGVTVGSGVKIQNGVSVYHGVTIGDEVFLGPHCVFTNDFRPRAFNSNWQIT
                     KTQVRRGASVGAGAIIICGIELGEYCMVGAGAVVTKDVPPHGLVLGNPARLVGYVCQC
                     GERSSEVGLKTKTYFCETCSSKEQHVERDL"
     gene            complement(781..1788)
                     /locus_tag="PLH57_04380"
     CDS             complement(781..1788)
                     /locus_tag="PLH57_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRK01878.1"
                     /translation="MKVLLCGLGSMGSNHLRVLMSLNQEFDVVGVVDANPKRLADAKS
                     LHGVTGFTNISEAVETTKPEAVVVVTPTETHFEICKEILEKGIALFVEKPITKNVKQG
                     LELARLADSKKIPFMVGHIERFNPAVVAVRELIRRGDVGDLISVSVKRVGGNPRDVKG
                     AGDVLVDLAVHDIDIVSWLTGQTPKLLDCVGHKSQAIDSASMLFSCGATTVDMHVNWV
                     TPVKIRQIQVTGTKGFIEVNLITQQVVWTKQNPVLIDFSTEKSQFFFDEYLLSFAAPD
                     KIEVGILKREPLREEMRAFWKSVTTGAPMPVTAHEAVQALDLALQARAKVEKQLASSQ
                     S"
     gene            complement(1794..3035)
                     /locus_tag="PLH57_04385"
     CDS             complement(1794..3035)
                     /locus_tag="PLH57_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HRK01879.1"
                     /translation="MNSTHSGVQPERSRLAPISQQSLDAKLGFTWPAPESRKRCQRCV
                     YDEATPAITFDENGICNYCKVHDEFEREYPTGEEGERRLREIADKIKIAGRGKKYDCI
                     IGVSGGCDSSYMLVKAKELGLRPLAAHFDNTWNSAIATENIRRMLKPLEIDLFTIVVN
                     NREYDDLYASFFRSGVTDLEIPTDIGLAATLYQAAEKYGIQYVLEGHSFRTEGIAPLG
                     WTYMDGKYIQSVHAQFGTQPLKTYPNMLFFDFMKWTVFRRIRKIRPLYYIDYHKENAK
                     KMLAEKYGWQWYGGHHLENRFTAFYHSFYLPRRNGSDTRLLGFAAMVRSGQMTREEGI
                     EQIRKAPEFDIELVELVKKRLKFSDEEFIRLMKAPVKSYRDYKTYKPLFERLRPFFWM
                     LYRLDLVPKSFYQKYTSKSEV"
     gene            complement(3032..>3883)
                     /locus_tag="PLH57_04390"
     CDS             complement(3032..>3883)
                     /locus_tag="PLH57_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRK01880.1"
                     /translation="FELPAGSINQGMIRARVIPCLLVTGEATLVKTVGFARAQYIGDP
                     VNTVSIFNDLEVDEIAILDIEASRQDREPQFELIREITANCFIPLSYGGGVKTIDHMK
                     RLFDVGVEKIILNTALFSDPDLVSGASEHFGAQAIVASIDVKQTLLGRRKVFRRLGQA
                     VKHSRARSEQTNDLLRSLDFDDPADFARYVQALGVGEIFLNSVDRDGTFEGYDLDLIR
                     AVVKASDVPVVACGGAKNFRDLRTPVLQAGASAVAAGSIFVYQNQNRSVLVSFPDRSE
                     LKEIFAP"
BASE COUNT         1021 a         1027 c          831 g         1004 t
ORIGIN      
        1 gagtgccatt agcgccatta caagaccgtc ggttccactc gccacggtaa tgcaatgtcg
       61 agctcctaca tactgtgcga gtttagcctc gagttcaacg acctcgggcc ctagaatgta
      121 ctgaccatgc tgcaacacgc ggcgaatcga ctcgtcaatt cgactgccta ctaacgcata
      181 ctgcgcctta agatcgataa actgaatttt ttcagaggtc tctctcaaca tgctgctcct
      241 tcgatgaaca ggtctcgcaa aaataggtct ttgtttttaa tccgacctcg ctcgaacgtt
      301 ccccacattg gcaaacataa cccacaagcc ttgctggatt ccccaatacg agtccatgag
      361 gcggcacatc cttggtaact accgcccccg ctccaaccat gcagtattca cctagctcga
      421 ttccacaaat gataattgca ccagccccaa cgctcgcacc gcgacgaacc tgagttttag
      481 tgatttgcca gttcgaatta aatgcgcgag gtctaaaatc gttggtaaat acacagtggg
      541 gtcccaaaaa cacttcgtca ccaattgtca caccgtgata taccgagacg ccattttgaa
      601 ttttcacgcc acttccgacc gtcacgccat ggtcaacgta tacatcttta gatagaatac
      661 agttgcggcc gatattagca ctttccctta tctggcagtt attccaaaca cgtgtgccct
      721 caccaatctc ggctttttcg gagacatcag cggttttatg aatggtgacg tttttccact
      781 tcatgactgt gaagatgcca attgtttttc aaccttcgcc cgagcttgaa gcgccaaatc
      841 aagcgcctgc accgcttcat gagcagtaac cggcatcggc gcccccgtcg ttacggactt
      901 ccaaaacgct cgcatctctt cacgtagtgg ctctcgtttt aggatgccca cctcgatttt
      961 atcgggcgct gcaaacgaaa gaagatactc atcaaagaag aattgacttt tttccgtcga
     1021 aaagtcgatc aataccggat tttgtttggt ccaaacgact tgttgagtaa taagatttac
     1081 ctcgataaaa cccttggttc cagtaacctg aatttggcga attttaacag gcgtcaccca
     1141 atttacgtgc atatcgaccg tcgtcgcacc acagctaaaa agcatagatg cactgtcgat
     1201 cgcttgactc ttgtggccta cacaatcgag caacttggga gtttgcccag tgagccatga
     1261 aacgatatca atgtcatgca ctgcgagatc gaccagtacg tcgcccgccc ccttcacgtc
     1321 gcgcggattg ccaccgaccc gtttgacgct caccgaaatg agatctccta cgtctccacg
     1381 tcgaattagt tcacgcaccg caactactgc tggattaaaa cgttcaatat gtccgaccat
     1441 gaatggaatt ttttttgaat cggctaatcg tgccagttcc aatccttgtt ttacattttt
     1501 ggtaatcggc ttttcaacaa acagtgcaat acctttttcg agaatttctt tgcaaatttc
     1561 aaaatgggtc tcagtaggag taacgacgac taccgcctca ggttttgtcg tttcaacagc
     1621 ctccgaaata tttgtaaaac cggtcactcc atgtaaagat tttgcgtcag caagtcgctt
     1681 cgggtttgcg tccaccactc caacaacgtc aaactcctgg tttaaactca ttaaaacgcg
     1741 cagatggttg gaccccatgc taccgagtcc acagagaagc actttcatgt taattaaacc
     1801 tcagatttcg aagtgtactt ttgataaaag ctcttgggca ctaaatccaa gcgataaagc
     1861 atccaaaaaa acggtctgag tcgctcaaac aaaggtttgt acgttttgta atctcggtaa
     1921 cttttgaccg gagctttcat aagacgaata aattcttcgt cagaaaattt taaccgtttt
     1981 tttaccaatt caaccaactc gatatcaaat tccggagctt tacgaatttg ctcaataccc
     2041 tcttcgcgcg tcatctgacc cgaacgaacc attgccgcaa aacctaaaag tcgtgtatcg
     2101 gacccattgc ggcgtggaag ataaaaagaa tgataaaacg ctgtgaaacg attctcgagg
     2161 tgatgaccgc cataccactg ccatccgtac ttttcggcga gcatcttttt ggcattttct
     2221 ttgtgataat cgatataata aagcggtcga atttttcgaa tccgtctaaa aactgtccac
     2281 ttcatgaagt cgaaaaacaa catgttcgga taggttttta gcggttgagt gccaaattga
     2341 gcgtgaactg actgaatgta cttgccgtcc atgtaggtcc agccaagagg tgcgatgcct
     2401 tcggtacgaa acgagtgccc ctccaacaca tactgaattc cgtacttttc agccgcttgg
     2461 tatagtgtgg cagcgagccc gatatctgtg ggaatttcaa gatcggtaac gccagatcta
     2521 aaaaatgagg cgtataaatc atcgtactcc cgattattta caacgatcgt gaacaaatcg
     2581 atttcgagcg gcttgagcat gcgtcgaata ttttcggtcg caatagcgga gttccaagtg
     2641 ttatcgaaat gggcagctag cggacgaagc cccagctcct ttgctttgac aagcatgtaa
     2701 gacgagtcac agcctccgct taccccgata atacagtcgt atttttttcc ccgtccggcg
     2761 atcttgattt tatctgcgat ctcgcgcaaa cgccgttcgc cttcttcgcc ggtagggtat
     2821 tctcgttcga actcatcgtg aactttgcaa taattgcaga tgccgttttc atcaaacgta
     2881 atggcgggag ttgcctcgtc gtacacacaa cgctgacagc gtttgcgtga ctcgggggcc
     2941 ggccaagtga aaccgagctt ggcatcaaga ctctgttgac tgatcggggc taaccgagat
     3001 cgctctggtt ggactcccga atgcgttgag ttcatggagc aaaaatctcc ttcaattcag
     3061 accggtctgg aaagctgacc aaaacggacc gattttgatt ttgataaaca aatatactgc
     3121 cggcggcaac ggcgctcgcg ccagcttgca atactggagt ccttaagtca cggaaattct
     3181 tcgccccgcc acaggctacc acgggcacat cggaagcttt taccactgct cgaataaggt
     3241 ccaaatcata gccctcaaat gtcccgtctc gatcaaccga attgaggaaa atttcaccta
     3301 cgccgagggc ctgaacatac cgagcaaaat cggcgggatc atcgaagtct agactccgaa
     3361 gtaggtcatt agtctgctcc gatcgagcgc gggaatgctt caccgcctgg ccaagtcggc
     3421 gaaacacttt gcggcgcccc aatagcgtct gctttacgtc gatcgacgcc acgattgcct
     3481 gagcaccgaa atgttcactg gctcccgaga ccaaatcggg gtcagaaaat agagccgtat
     3541 ttaaaatgat tttttcgacc cccacatcga acaatctttt catatgatca atagttttta
     3601 ccccgccccc ataagacagc ggaataaaac aattggccgt aatctcgcga atcagttcga
     3661 attgtggttc acgatcctgc cgactagctt cgatatcgag aatagcgatt tcgtcgacct
     3721 cgagatcgtt gaaaatacta accgtgttaa cagggtcgcc aatgtactga gctctcgcaa
     3781 acccaactgt ttttaccagg gtcgcctcgc cagtaacgag caaacatgga attacccgcg
     3841 cacgaatcat gccctggttg atgctgccgg cgggcaactc gaa
//
LOCUS       DAOMXO010000156         3878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12788_length_3878_cov_4.744703, whole genome shotgun sequence.
ACCESSION   DAOMXO010000156 DAOMXO010000000
VERSION     DAOMXO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12788_length_3878_cov_4.744703"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /gene="deoC"
                     /locus_tag="PLH57_04395"
     CDS             <1..572
                     /gene="deoC"
                     /locus_tag="PLH57_04395"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRK01881.1"
                     /translation="QRLASKTPAPGPAVPPVPIAVVGFPLGAMHTKAKCEETRVAVEE
                     GAQEIDMVLAVGWLKEQKFEAVFQDIAAVVNQCGPNVPTKLIIETALLNDVEKIRACV
                     LGVWAGVKFVKTSTGFAANGATVSDVALMRNVVGDQVGVKASGGIRTAADALAMLQAG
                     ATRLGVSSSVAILNELRTGTSSAGSSCSY"
     gene            complement(566..1744)
                     /locus_tag="PLH57_04400"
     CDS             complement(566..1744)
                     /locus_tag="PLH57_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01882.1"
                     /translation="MLKYSKIKFYFAIWCGTLAAGVFAGIATAGERSQAGADLFCRQI
                     MEAPIKGNRDVQVQLSERLVELEQALRVLGAAPEVRVQVYDKYIDTLEVLGRHQEAFV
                     AFDRLLKGYSRVMSEMSRSSTDYNYDSIGVVVRDTEPELTHLWGIIEPELEHGRTLAV
                     GRAELLNDLLKLLRDGNLRSTLLSWDIGALQIRLRDLDFTLVPAILLEQQPNSLSDNE
                     DNDDSTEIRTPGMFLLVKDVLGRPIDDSFASDESQLIDMVEEHFQAFAIHRLHTIDPK
                     AFGRLNEIFGEQAGSVARWIIKNLKYQLNLAARDELYWVLNGDLKVRVPVERLIREIT
                     DALRVASSKNWSLVDAPSSEALFSGELMPAALKELLLNYREYRRPIVRLKKMRKKSDQ
                     "
     gene            complement(1789..3036)
                     /locus_tag="PLH57_04405"
     CDS             complement(1789..3036)
                     /locus_tag="PLH57_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRK01883.1"
                     /translation="MKLAKRICTISLLLALLTATYSRAEESLPVSDKARTCARFLSKT
                     SFWHLASKVKFGHYRLVREIMESRVLEAPELRKRKEKVGEGHLLLTTPKGGKEFYLVE
                     FTENEALIRTVDGKYYRIQIRDSRMLPAGQDGPYRPLYALGDGSHTFFETTPLSARGI
                     FEAIATKNKIDSQVLAQAKPESFEFVEFGPGKFMMGSPKEETSRHSDERQHEVIITRP
                     FEMAKGPVTQALWYTVKAANPARFKDSGIAVAGDKVDPNKPIENVSWEDAQAFILALN
                     ESQSEYTYRLPTEAEWEYAARAGTTTAYSFGNNPKDMGDYAHFSNQRTTDIATRKPNP
                     AGLFDMHGNVWEWVQDWYDSNYHKGNNVDPIGPSSGSRRVLRGGSWYYVARELRSARR
                     YYAHPRVRSGVVGFRLVRTRRGQ"
     gene            3161..>3878
                     /locus_tag="PLH57_04410"
     CDS             3161..>3878
                     /locus_tag="PLH57_04410"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRK01884.1"
                     /translation="MSSQNTKIERVVLIVLDGVGCGEMPDANEYGDTGSNSLGNLVRV
                     EQNLKLPNLERWGMGNITPMGDAAHPRKPHLCTAGFGKLAELSKGKDTTSGHWEMAGI
                     VTETAFTTYPNGFPESAVREWVTACNLPGVLGNKAASGTEIIKELGEEHIKTGKPILY
                     TSADSVWQIAAHEDDLIFGLDRLYEVSKVARKIADKLQLSRVIARPFIGKTSADFSRT
                     HRRKDYSQLPPAKTTMDLLKE"
BASE COUNT          965 a         1023 c          949 g          941 t
ORIGIN      
        1 cacaacgact agcatccaaa acaccagccc ctggaccagc tgttccccca gttccgattg
       61 cggtggtggg ttttccgttg ggggccatgc acacaaaggc taaatgcgaa gaaactagag
      121 ttgccgtcga agaaggcgcc caggaaatcg acatggtgtt agccgtaggt tggctcaagg
      181 agcagaaatt cgaagcggtt tttcaagaca tcgcggccgt tgtcaaccag tgcggcccta
      241 atgttccgac aaagctaatc atcgaaaccg cgctactcaa tgatgttgaa aaaattcgtg
      301 cgtgcgtatt gggcgtctgg gcgggagtta agtttgtcaa aacctccacc ggttttgcgg
      361 caaatggcgc gacagtttca gatgtcgcac tcatgcgaaa cgtcgttggc gatcaggttg
      421 gagtaaaggc cagtggtggc attcgaacgg cagccgacgc acttgcgatg ttgcaagccg
      481 gagccactcg tctcggcgtt agttcttcgg tagccatact gaatgagctt cgaaccggga
      541 cgtcgtctgc aggctcatcc tgcagctatt gatcgctttt ctttcgcatt tttttaagcc
      601 gaacaatggg acgtcgatac tctctataat tgagcaatag ctctttgaga gcggctggca
      661 tcagttcgcc gctaaagaga gcctcggaac ttggcgcgtc cacaagactc caatttttag
      721 agctcgcaac ccgcagagca tccgttattt cgcgaattaa gcgttcaacg ggcactctta
      781 cttttaggtc tccgttcaag acccaataga gttcatcacg agccgctagg tttaactggt
      841 acttcaaatt tttaataatc caacgagcaa cgctacccgc ctgttcaccg aatatttcgt
      901 tgagccgtcc gaacgctttg gggtcaattg tatgcagcct gtgaatggca aacgcctgaa
      961 agtgctcttc aaccatatcg attaactgac tttcatcgga agcgaacgaa tcatcaatcg
     1021 gacgcccaag cacatcttta accaaaagaa acatccccgg ggttcgaatt tctgtagagt
     1081 cgtcgttatc ttcattgtcg gatagggaat ttggttgctg ttccaacaga atcgccggga
     1141 ctagtgtgaa atctagatcc cgcagacgaa tttgaagagc accgatatcc caactcaaaa
     1201 gcgtggaacg caaattgccg tctcgaagca gcttgagtag gtcatttagt agctcggccc
     1261 gccccactgc gagtgtgcgc ccgtgttcta gttcgggttc aataatgccc cacaaatggg
     1321 ttaactcagg ctcggtgtcg cgcacgacta cgccgattga atcgtaattg tagtcggtac
     1381 tgctgcgaga catttcactc atgactcgag agtagccctt taaaagacgg tcgaacgcca
     1441 caaatgcctc ctggtgtcgt cctaacactt cgagcgtatc aatgtatttg tcgtaaacct
     1501 gtactcgtac ctctggagcg gctcccaaaa cccgcaaagc ttgctccagc tcaaccagac
     1561 gctccgagag ctgaacctga acgtctctat ttcctttaat cggagcttcc atgatttgcc
     1621 ggcaaaaaag gtcagcccct gcctgactgc gctcaccggc ggtcgcgatt cccgcaaaaa
     1681 caccggcggc cagggttccg caccaaatcg caaaatagaa ttttattttc gaatatttca
     1741 acacgcgagg ccctatacca taccccaacg taaattacac tttgcgggct actgtccgcg
     1801 ccgggtcctc acgaggcgga aacccacaac gccggaccga accctagggt gagcgtaata
     1861 gcgccgggca gaccggagct ccctggcaac gtagtaccag gaaccgccac gcaacacgcg
     1921 gcgcgagccg ctagaagggc cgatcggatc gacattgtta cctttgtggt agttgctgtc
     1981 gtaccaatcc tgcacccatt cccagacatt tccatgcata tcaaataggc ccgctggatt
     2041 tggtttgcgg gtagcaatat cagttgttcg ctgattgcta aagtgtgcgt aatcgcccat
     2101 gtcttttgga ttattgccaa aggaatatgc cgtagtcgta ccagcgcgag ctgcgtattc
     2161 ccattccgct tctgttggta gacgataagt gtattcactc tggctttcat tgagcgccag
     2221 aataaacgct tgagcgtctt cccagcttac gttttctatt ggtttattcg gatcaacttt
     2281 atcgccagct actgcaatac cgctatcttt aaaccgcgca ggatttgctg cctttactgt
     2341 ataccagagc gcttgcgtga ccgggccttt tgccatttca aaaggtcggg tgatgatcac
     2401 ttcgtgttgt ctttcatcag agtgacgact cgtttcctct tttggactgc ccatcatgaa
     2461 ttttcctgga ccaaactcca cgaactcgaa actctctggc tttgcctggg ctagcacttg
     2521 cgaatcgatt ttgtttttcg tagcaattgc ctcaaaaatg ccccgcgcgc taagaggcgt
     2581 agtttcaaaa aaagtatgac ttccatcgcc caacgcataa agcgggcggt aagggccgtc
     2641 ttgtccggcg ggaagcattc gtgaatcgcg gatttgaata cgataatatt tgccgtctac
     2701 cgtgcggatg agcgcttcat tttctgtaaa ttccacgaga taaaactctt tgccaccctt
     2761 tggtgtcgta agaagcaagt gaccctcacc tactttttct ttgcgtttgc gaagctcggg
     2821 agcttcaagc actcgtgatt ccatgatctc gcgcaccaaa cggtagtgac caaactttac
     2881 tttgctcgct aaatgccaaa acgatgtttt ggaaagaaac cgtgcgcacg ttcttgcctt
     2941 gtcgcttacc ggtaaagatt cttcagcgcg cgaataagtc gcggtgagca atgcaagcaa
     3001 aagactgatt gtgcagattc gtttggccag cttcatgcga tgcgacaata cattaaaaac
     3061 gacacaaagt aaactaaatt agtcagttaa tatattcaac cacgaaccgc ttgcctctgc
     3121 gccggggtca tgctcgtata gggcccattg gtgatttgaa atgagctccc agaacacaaa
     3181 aatcgagcgg gtagttttga tcgttctcga cggcgtaggg tgtggagaaa tgcccgatgc
     3241 caacgaatac ggcgacacgg ggtccaacag tctaggaaac ctggttcgag tggaacaaaa
     3301 ccttaaattg ccgaacctcg aacgctgggg catgggaaac atcaccccaa tgggcgatgc
     3361 ggcccacccc cgaaaacctc acctttgtac cgccgggttt ggcaaattgg cggaattgtc
     3421 gaaaggcaaa gacaccacgt cgggtcactg ggaaatggcg ggaattgtaa ccgagaccgc
     3481 ctttacgact tatccgaacg ggttcccgga atcggcagtt cgtgaatggg tcacagcctg
     3541 caatctgccc ggagtcttag gcaacaaggc agcaagcggc accgagataa tcaaagaact
     3601 aggcgaagaa cacattaaga ccggcaagcc aattctctac acttcggccg attcggtatg
     3661 gcaaatcgcc gcgcacgaag acgacctgat tttcggtctc gaccgactct acgaggtctc
     3721 aaaagttgca cgtaaaatcg ccgacaaact tcagctttcc cgtgtcattg ctaggccatt
     3781 tattgggaag acctcagcgg acttttcccg cactcatcgg cgcaaggatt attcacagct
     3841 gcctccggca aaaaccacga tggatttgct gaaggaac
//
LOCUS       DAOMXO010000157         3876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12804_length_3876_cov_4.713164, whole genome shotgun sequence.
ACCESSION   DAOMXO010000157 DAOMXO010000000
VERSION     DAOMXO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12804_length_3876_cov_4.713164"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /gene="aroF"
                     /locus_tag="PLH57_04415"
     CDS             <1..817
                     /gene="aroF"
                     /locus_tag="PLH57_04415"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014953694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRK01885.1"
                     /translation="RDHRTIVKAAGVEFGGKLIPIFAGPNAVENESMIMRTAEAVKMA
                     GAHFLRAGAFKPLSFPYRSEKYFELREEGLKLLLKAKAAFQIPIITEIMHETKVDIVA
                     EVADILQVGARNMQNYPLLEAVARTSKPIMLKRHYGASLRDWLGAAEYILHAGNPNVI
                     LCERGVVVPHTHRATSRFLLDLQVIPAAQEVTHLPVVTDPSHATFWKPWVKPMALSSI
                     AAGADGVMIEVHPDPENAAVDPLQPIDFRDFKGLMKDMERVANAIDRTVGVSA"
     gene            814..2178
                     /locus_tag="PLH57_04420"
     CDS             814..2178
                     /locus_tag="PLH57_04420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK01886.1"
                     /translation="MNKKLVSIIVRTKNEERWISAALQAVFSQTYPNFEVILVDNLSN
                     DRTLEKAKKFDVRVVSIEDFLPGKAINLGIRQSKGEYVVCLSGHCIPVDDKWLETLVN
                     TLEEDANFAGVYGRQQPLSYSSDLDKRDLIITFGLDRRIQHKDPFFHNANSILRRSIW
                     DQIPFDEAATNIEDRIWASAVLKKGLKIVYDPDASVYHYHGIHHGRDLGRAKKIVKIL
                     ETLESKTLRNPGKSEVRKVAAVIPVRGSDLEVFAGRPLLEYSIRSSCASKLVDQTVVV
                     SEDAKALELARQLGADICLERPAELSFDYRDITDTLVFADAALAAKGLAPDVVVYLSV
                     NSPFRRKQLIDSMLQKMFENNYDSIHAAFPENRTCWIEQNGELVLVGEEFVPAKYRDP
                     LYISLSGLATCVHSDVLRSGTRLGKNVGLFQVDDSISLMDIKKIPNKEIAGNLMSAWD
                     KEFG"
     gene            complement(2187..3143)
                     /locus_tag="PLH57_04425"
     CDS             complement(2187..3143)
                     /locus_tag="PLH57_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRK01887.1"
                     /translation="MQPTNSRNALVIGYGSIGSRHARILESHCKSVSLVTKQDAGKFR
                     TYDSIVQAFSRDHFDYVVIANNTSDHFDAYRNLVAVGYTGALLIEKPVFDKRPEAPIS
                     SPFSLCGVGYNLRFLPVLQRLKELLGNDTIVTVNVYVGQYLPSWRPKRANGTDSYSAK
                     KKLGGGVLRDLSHEIDYIQWIFGEWTNLTALGGKISDVTIDSDDAYSILLKTKRCSNV
                     VVHMNYLNRSAKRFMIVNSNKGTWIADLITGQLEIQRFTQDEQIIEKHGVNSDFTYSL
                     MHKHLLAGETSICCTLNEAMQTLAAIEACEVASSTKTWISAV"
     gene            3265..>3876
                     /locus_tag="PLH57_04430"
     CDS             3265..>3876
                     /locus_tag="PLH57_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRK01888.1"
                     /translation="MSKEQIIQSIIDGIKEVLPGSGSPNASFELHEPRFTGNELNYLK
                     QCVETGWVSSLGEFVTKFEKDLSNFTGASHAIATSNGTAALHTCLMLVDVKPGDEVLV
                     PALTFVATGNVVAYCAATPNFYDSEPHTLGVNVQKLREYLASNCRVVGSDCVNKATGR
                     RIRALIAMHTFGHPCDLAPLKDLCLDFKIRLIEDAAESLGSYYK"
BASE COUNT         1092 a          801 c          951 g         1032 t
ORIGIN      
        1 cagggaccat agaaccatcg tgaaagcggc tggtgtagaa ttcggtggaa agctaattcc
       61 gattttcgcc gggcctaatg ctgtcgagaa tgaaagcatg attatgcgaa cagcagaagc
      121 agtaaaaatg gctggtgcgc atttcttgag ggcgggcgcc tttaaacctt tgagttttcc
      181 ctatcgaagt gaaaaatatt tcgaactgcg cgaagagggg ttgaaacttt tacttaaggc
      241 gaaggcagct tttcaaattc cgattatcac agaaattatg cacgaaacaa aggtggatat
      301 agtggctgaa gttgccgata ttcttcaggt tggtgcacga aacatgcaaa actacccact
      361 gctcgaagca gtagccagga cctcaaaacc gattatgctt aagagacact acggtgcttc
      421 gcttcgcgat tggttgggag ctgccgagta tatattgcat gcgggtaatc cgaatgtaat
      481 tttatgtgaa cgtggggtag tggttcctca cacacatcgc gcgacttcga gatttctttt
      541 agaccttcag gttataccgg cagcgcagga ggtaacccac ctcccagtag tgacagaccc
      601 ttcacacgct acgttttgga agccatgggt taagccaatg gcattgtcgt ctattgcagc
      661 aggcgcagat ggcgtaatga tagaagttca cccggatcca gaaaatgcag cagtagaccc
      721 attacagccg atagatttta gggacttcaa aggtcttatg aaggacatgg agcgcgtagc
      781 taacgcaatt gatcgaacgg tcggtgtgtc tgcgtgaata aaaagctagt ttcgataatt
      841 gtgcgtacga agaacgagga gcgctggatt tctgcagcct tgcaagcagt gttttctcag
      901 acttatccga attttgaagt aatcctagta gacaatttaa gtaatgatcg aacgctagag
      961 aaagctaaaa aattcgatgt tagggtcgtt tccatagaag attttctgcc gggtaaagca
     1021 attaatctcg gaatacgaca gtcaaaaggt gagtatgtgg tgtgcttgtc gggtcattgt
     1081 attccagttg acgataagtg gcttgaaact ctagtaaaca ctctagaaga ggatgcgaat
     1141 tttgcgggcg tttatgggcg tcaacaaccg ctatcctata gttcagacct tgataaaaga
     1201 gatttgataa ttacgtttgg actggaccgt cgaattcaac ataaagatcc tttctttcac
     1261 aatgccaaca gcattcttcg aagaagcatt tgggaccaaa ttccatttga tgaggcggct
     1321 acgaacatcg aagatcgaat atgggcaagc gctgttttga aaaagggtct taaaattgtt
     1381 tatgacccgg atgcgagtgt ttaccactat catggaatcc atcacggtag agatctggga
     1441 cgagcaaaaa aaatcgtcaa aatactcgag accttggagt ctaagacgct tcgaaaccca
     1501 ggtaagtccg aagtaagaaa agtcgcagcg gtgattcctg tgcggggttc agatcttgag
     1561 gtttttgcag gacgcccgtt gctcgaatat tcaatcagaa gctcatgtgc gagcaaactc
     1621 gttgatcaaa cggtggtagt gtcggaagac gcgaaagctc tcgaattggc acgtcagctt
     1681 ggtgcggata tatgtttaga acgaccggcg gaactttcat ttgactacag ggatataacg
     1741 gacacgttgg tatttgcaga tgcggcttta gcagcgaagg gcctcgcgcc agacgtggtc
     1801 gtctatcttt cggtgaatag cccgtttaga agaaaacagc tgatagactc aatgcttcaa
     1861 aaaatgtttg aaaataatta cgacagcatc catgcagcat ttccagagaa tcgaacatgc
     1921 tggatcgaac aaaatgggga actcgtgctc gttggcgagg agtttgttcc agcaaagtac
     1981 agggaccctc tttatatcag cctttcaggg cttgccacat gcgttcactc agatgttcta
     2041 agaagtggga caagactggg taaaaatgtt gggctttttc aagttgatga ctcgatttcg
     2101 ctaatggata taaaaaaaat accaaacaaa gaaattgcgg gaaatttaat gagcgcctgg
     2161 gacaaggagt tcggctagtg ccaaattcaa acggcggaaa tccatgtttt cgtagaagac
     2221 gccacttcgc acgcctctat ggccgctaaa gtctgcatgg cttcgtttag agtgcaacaa
     2281 atagaagtct cgcccgctaa caaatgcttg tgcataagag agtatgtaaa gtccgaattg
     2341 acgccgtgtt tctcgataat ttgttcgtct tgagtaaatc tttgtatttc cagttggcca
     2401 gtaattaggt ccgcaatcca cgttcccttg tttgagttta cgatcatgaa tcttttagcg
     2461 gaacgattga gataattcat atgaaccacg acatttgagc accgtttggt ttttaggagg
     2521 atggaatacg cgtcgtcgct atcgatcgtt acgtcgctaa ttttacctcc gagtgctgtt
     2581 aaatttgtcc attctccaaa aatccactgg atataatcaa tctcgtgact cagatcccgg
     2641 agtacgccgc cacccaattt tttttttgcc gaatatgaat ctgtaccatt cgctcttttg
     2701 ggtcgccacg atggaaggta ctgcccgaca taaacattta ccgtaacaat agtgtcattg
     2761 cctaaaagct ctttgagtcg ttggaggact ggaagaaatc gtaaattgta tccaacacca
     2821 catagcgaaa acgggctact tatgggggcc tctgggcgtt tgtcgaaaac tggcttttcg
     2881 atcagaaggg caccggtgta accaaccgct acgagatttc gatacgcatc gaagtggtca
     2941 gatgtattgt ttgcgatgac tacataatca aaatgatccc gactaaatgc ctgcactata
     3001 gagtcatagg tgcggaattt tcccgcatcc tgtttggtta cgagcgaaac agatttacaa
     3061 tgagactcta gaattcgagc gtgtcgactt ccgatcgagc cgtaacctat taccaaggcg
     3121 ttacgagagt ttgtgggctg catgcggaca gtctattgat tgccagtttc gatttcaatg
     3181 gtcatatccg aaatccgaag attaacatga gtgcgaatta gataatttat attggaacga
     3241 gaatgagcta aagatgaggc catagtgtcg aaagaacaaa tcattcaatc aataattgac
     3301 ggtatcaaag aagttctgcc aggttcaggc agtccgaacg cgtccttcga acttcatgag
     3361 ccacgtttta ccggcaatga gttgaactac ttgaagcaat gcgtcgagac gggttgggta
     3421 tcttccctcg gtgaatttgt aacgaagttt gaaaaagact tgtcgaattt tacaggcgct
     3481 tcccacgcga ttgcaacgtc caatggaact gctgcactac acacttgtct aatgctcgtg
     3541 gatgtaaagc cgggagatga agtgttggtt ccagcgctta cctttgtagc tactggaaat
     3601 gtggtagctt attgtgccgc tacaccaaac ttttatgact ctgagcccca cacccttgga
     3661 gtgaacgttc aaaaattgcg cgagtacctc gccagtaact gtcgggtagt gggtagcgat
     3721 tgtgtaaata aggctacggg ccgaagaatt agagccttaa tcgcaatgca tacatttggg
     3781 cacccgtgcg atttggctcc actcaaagat ttgtgcttag attttaaaat tcgacttatc
     3841 gaagacgccg cggaatcgct cggatcatac tacaaa
//
LOCUS       DAOMXO010000158         3865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12869_length_3865_cov_4.923622, whole genome shotgun sequence.
ACCESSION   DAOMXO010000158 DAOMXO010000000
VERSION     DAOMXO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12869_length_3865_cov_4.923622"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLH57_04435"
     CDS             <1..319
                     /locus_tag="PLH57_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRK01889.1"
                     /translation="LLAVIADAQEKSKGKAIFDFRCASCHGLQGGGGIGPNLVDKFWM
                     NGNGKPISVAQLIKDGVPAKGMPPWGAILSESELYAVTAYVHSMKGSNPVNAKEPQGN
                     EYP"
     gene            335..1720
                     /gene="ccoG"
                     /locus_tag="PLH57_04440"
     CDS             335..1720
                     /gene="ccoG"
                     /locus_tag="PLH57_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRK01890.1"
                     /translation="MSQGINPRDLAKDRLTTTDAEGRRLHVYAQSVTGFYRNRRSLVQ
                     AVLVLVFLALPWWKINGQQAILLDVIHRRFTLFGIQFWAHDAPMLAFVAGAAVLSIAF
                     ITALFGRVWCGWACPQTVFIDGIFLRIERLIEGDAVKRRAFDARPLDIDKALLKAAKW
                     SLFTLVALVISHSFLGYFVGVERLAEIVRRPPAENIGLFAVMAGITGLLLFDFGWFRE
                     QFCTVVCPYGRFQSLLLDSHSLVIAYDAKRPDCVDCFRCVQVCPTGIDIRRGVQLECI
                     ACTACIDACDDVMTRLRLPPRLIKYASEESIQGRKTKFLRARTVVYFGLLVMIVTALT
                     AVVGSRRDLELFVARAAGAPYQEIAAAGTDGAGTKIVNRFRSEVTNRTFRPVVVTFTQ
                     GQEVVAGANIVLATQKLELAAGETRTVDLFVEFPKAELSEGKRKFQLTVTSDSELTKN
                     TEEVMLVGPYR"
     gene            1701..2501
                     /locus_tag="PLH57_04445"
     CDS             1701..2501
                     /locus_tag="PLH57_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRK01891.1"
                     /translation="MSAPIDSNLMAPLAVFVASLLGSSHCAGMCGGLVLFIGQNKLNL
                     VVYHAGRLLGYLTVGALAGELGKSVLQAKEMQGVAIAASALLGAVFVATGVRLVWPEI
                     RKRVAPRSGASFYRPFAKLNRMWSGAFHRLALVLKKRSHTWHGALAFGVSTALLPCGW
                     LYTFVLAAVATQSALLGAATLFAFWLGTVPALSAAPWVLRSVLKPISGSFPIVSGLLL
                     VAVGLYSWSGRSLQVIGNLGNSEPPGETINTNQPSCHGHHSGNSDGEP"
     gene            2518..3615
                     /locus_tag="PLH57_04450"
     CDS             2518..3615
                     /locus_tag="PLH57_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK01892.1"
                     /translation="MNYDLQTDQQNVFFRCVEDSNEAIMITNMRGNLVYVNPAWTAVY
                     KYTREEAIGQNPRILHSGMQSPEFYRSIWVAILDPELGHWKGELTNRAKDGTLVPVLL
                     SITPFRAPDHTIIGYMGRALDMTGQKDLEAKILQQDRLASVGMLASGLAHEVGTPLGI
                     VRGRAELLQMLVGDNQTVRSGLEVIVTQIDRISKLITALLRFSRGSDQVELRPTEILK
                     VVNEVLALIGGNFRQDSIELKIEVPPGLKASADFSHLEQIILNFAVNAAHAIQKAIKD
                     GRSSGHAMTVRAVEVADHVILSVTDTGCGITTEHRRRLFQPFFTTKDVGQGTGLGLAI
                     VAKLVSEMNGEITLDSVLNQGTTFSVRLNKA"
     gene            complement(3622..>3865)
                     /locus_tag="PLH57_04455"
     CDS             complement(3622..>3865)
                     /locus_tag="PLH57_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016916336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK01893.1"
                     /translation="TPDTVNAEHFFGSATADFPTVEALEKRYIKLVLEKTGGRKDRAA
                     HILGINRRTLYRKEREYGFLSADAPEEEVISDSETY"
BASE COUNT          900 a          901 c         1052 g         1012 t
ORIGIN      
        1 gctgttggcg gtcattgcag atgcgcaaga aaagtcgaaa ggaaaagcta tcttcgattt
       61 tcgctgtgct tcttgccatg gattgcaggg cggcggagga attggtccta atctcgtcga
      121 caagttctgg atgaatggaa acggaaaacc catctcggtg gcgcaattaa ttaaagatgg
      181 cgtgcccgct aaggggatgc cgccgtgggg cgcgatttta tcggaaagtg aactctatgc
      241 ggtaaccgct tatgtgcatt ctatgaaagg cagtaatccg gtgaacgcca aagaaccgca
      301 gggcaacgaa tatccatagg taaagctaca ataaatgtct caaggtatta atcctcgtga
      361 tcttgctaaa gaccgtctta ccactaccga tgccgaaggt cggcggttgc atgtttatgc
      421 acaaagtgta acagggtttt atcgcaaccg tcgatcgttg gtgcaagccg ttctggtgtt
      481 ggtatttctt gcgttgcctt ggtggaaaat caacggccaa caagccattt tgctagatgt
      541 aattcaccgg cgctttacgc tatttggtat tcagttctgg gcgcacgatg caccgatgtt
      601 ggcttttgtc gcgggtgccg cggtattaag tattgcattc atcactgccc ttttcgggcg
      661 cgtgtggtgc ggctgggctt gtccccaaac ggtgtttata gacggaatat tcttacgaat
      721 agaacgcttg atcgagggtg atgcagtaaa acgacgagct ttcgacgctc gaccactaga
      781 tatcgataaa gcactgctca aggcggcgaa atggtctttg ttcaccttag ttgcgctggt
      841 tatatctcat agttttctgg ggtacttcgt cggtgtcgag cggctcgcgg aaatcgttcg
      901 gcgcccacca gctgaaaaca ttggactctt tgcggtgatg gcgggcatca ccggcttgtt
      961 actttttgat ttcggttggt ttcgtgaaca gttttgcacg gtcgtttgtc catacgggcg
     1021 ctttcagtca cttcttctag attctcattc tctggttatt gcttacgacg cgaaacgccc
     1081 cgattgcgta gattgttttc gctgcgtgca ggtgtgcccc acgggtatcg atattcgacg
     1141 tggagtacag ttagaatgta ttgcttgcac ggcctgtatt gacgcttgtg acgacgtaat
     1201 gacgaggttg cgtctacctc cgcgcctcat taagtacgcg agcgaagaat cgattcaggg
     1261 tcgaaaaacc aaattcctgc gggcgcgcac cgttgtatat tttgggttgc tggtcatgat
     1321 cgtaaccgcc cttactgcgg tagtggggtc tcgacgcgac ctagagcttt ttgtcgcgcg
     1381 agcagcgggg gcgccttatc aggaaattgc cgccgcggga actgacggcg ctggtactaa
     1441 gattgtgaat cgatttcgat ctgaagtgac caatcgaaca tttcgcccgg tagtggtcac
     1501 ctttactcag ggacaagaag tcgtagctgg tgccaacata gtacttgcga cccaaaaact
     1561 cgaactagcc gctggcgaaa cacgcacggt cgatttgttt gtcgagtttc cgaaggcgga
     1621 actaagcgaa ggaaaaagaa agtttcaatt aaccgtaacg agtgattcag agctcactaa
     1681 aaacacggaa gaggtaatgc ttgtcggccc ctatcgatag taacttaatg gcgccccttg
     1741 ccgtgtttgt cgcgagcctt cttggcagct ctcactgcgc cggaatgtgc ggcggactcg
     1801 ttttgttcat tggacaaaat aaactaaacc tcgtggtgta tcacgcgggg aggttgttag
     1861 gttatctgac cgttggtgca ctcgccgggg aacttggaaa aagtgtactc caggctaaag
     1921 aaatgcaagg ggttgcgata gcggcgagcg ccttgttggg tgccgtattc gtcgccacgg
     1981 gcgtgcggtt agtgtggccc gagattcgga aacgagtggc tcctcggagt ggggcctcgt
     2041 tttaccgtcc gtttgcaaaa ttaaatcgga tgtggagtgg ggcatttcat cgattggctt
     2101 tggttctaaa aaaacgatct cacacctggc atggagcgtt ggcgtttgga gtaagtaccg
     2161 cgttgcttcc ttgtggttgg ctctacacct tcgtgctggc agcagtggcc actcagagtg
     2221 ctttacttgg ggcagctacg ctatttgctt tttggttagg tactgtgccg gcgctaagtg
     2281 ccgctccttg ggttctacgt tccgttctaa agccgatttc tggaagtttt ccaatagttt
     2341 ccggcttgtt gttggtagcc gtaggtcttt attcgtggtc gggaaggtcg ttgcaagtta
     2401 ttggcaattt aggtaactct gagccgcccg gggaaaccat caatacgaac caacccagtt
     2461 gccacggcca tcacagtgga aatagcgacg gagagcccta aacttattcg gtagtaaatg
     2521 aattacgatt tgcagactga tcaacaaaac gtttttttcc gctgcgtcga agactccaac
     2581 gaagcaatca tgatcaccaa tatgcgcggg aacttggttt acgtaaaccc tgcctggacg
     2641 gcggtctaca aatacacacg tgaagaggca attggtcaga atccccgaat tctgcattcc
     2701 ggcatgcagt cgcccgagtt ctacagaagc atttgggttg cgatcttgga ccctgaacta
     2761 gggcattgga agggggaact caccaacagg gcaaaagacg gaactctggt gccagtgtta
     2821 cttagtatta cgccctttcg cgcgccagac cacacgatta tcggttacat gggccgggcg
     2881 ctcgacatga ccggacagaa agatctcgaa gcaaaaattc tacaacaaga ccgattagct
     2941 tcggtcggta tgttggccag cggacttgcc cacgaagttg gaactcccct tggcatcgta
     3001 cgtggccgcg cggaacttct tcaaatgctg gtgggcgaca atcagacggt gcggagtggt
     3061 cttgaagtaa tagttactca gatcgatcgc atttcgaaac tcatcaccgc gcttttacgt
     3121 ttcagccgag gttctgatca agtggagctg cgcccaaccg aaattttaaa agtggtcaac
     3181 gaagtgttgg ccctcattgg cggaaacttc agacaagatt ccattgagct caaaatcgaa
     3241 gtgccgccgg ggctaaaagc cagtgcggac tttagtcacc tggaacagat cattttgaat
     3301 ttcgcagtca atgcagccca cgctattcaa aaggcaatta aagacggccg gtctagcggt
     3361 cacgccatga ctgtaagagc agtagaagtg gcggaccacg taattttgtc agtgactgat
     3421 actggttgtg gtattacgac tgaacaccgc cgaaggcttt ttcagccgtt ttttaccacc
     3481 aaagatgttg gtcaaggaac cggactcggg ctggccatcg ttgcaaaact tgtttctgaa
     3541 atgaacggtg agattacatt agacagcgtg ctgaaccaag gcaccacgtt ttcagtgcga
     3601 ctgaacaagg cgtgatttaa attaataagt ctcgctatcc gaaataactt cttcttcggg
     3661 cgcatcggcg cttaaaaaac cgtattcccg ttctttgcga taaagcgtac gacgattaat
     3721 tccaagaatg tgagccgccc tgtctttgcg acccccggtt ttttctagca cgagcttgat
     3781 gtatcgtttt tccagcgcct ctacggtggg aaagtctgcg gtagccgatc cgaaaaagtg
     3841 ttcggcattc actgtgtctg gcgtt
//
LOCUS       DAOMXO010000159         3863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12883_length_3863_cov_3.906775, whole genome shotgun sequence.
ACCESSION   DAOMXO010000159 DAOMXO010000000
VERSION     DAOMXO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12883_length_3863_cov_3.906775"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..894)
                     /locus_tag="PLH57_04460"
     CDS             complement(76..894)
                     /locus_tag="PLH57_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRK01894.1"
                     /translation="MSFITLSSGGLERGKKIPFLNTIDMIWRILAAIVWFLVASLMAM
                     LLLPFRWRNPTVGHVFAKLLHTVGLRIFGLKVMVHNPERMDQRPCVYVANHQSNLDIF
                     TMSTIYPPDTVVIGKKELKWLPLFGWLFAGAGNVMIDRKNRTHSLSGFDDAAKAVRQR
                     GLSVWIFPEGTRNRGSAEMLPFKKGAFHLAVRAQIPIVPIVHQHLHRYFDYDKHWFNH
                     KDDVHIQVLEPVTTAGLGVGDIETLMATVRSKMDEVLKQPLYRPEPLAPASSKG"
     gene            1000..1437
                     /locus_tag="PLH57_04465"
     CDS             1000..1437
                     /locus_tag="PLH57_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRK01895.1"
                     /translation="MPYKRSKFLINRRFQLKFAFFVCSWIFALSMVYPIIIYNVFEYF
                     LKILSAPHDVLTVDKVKYVENQVVIVLGILQLLFLTITFMLSIFLSHRIAGPLFKLRR
                     AIDEVSRGNFDQRITFRKNDHFMELQDGFNEMVQHLGVRRWKE"
     gene            1465..3369
                     /locus_tag="PLH57_04470"
     CDS             1465..3369
                     /locus_tag="PLH57_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRK01896.1"
                     /translation="MANNSIFAILRIAIISAHLIFLATAGFAADEPIGANISLIEQIE
                     QRIVTDKEKRIPGLLEAYDLLSKGKTDLAINKLRLLATNREFSDFYHYLMGSAHYQKM
                     VQAAEAKKWITAISYGETASIHFNSAQTSLPYNTFESRIALYLGLTEVYLGQAYAKAK
                     KKDQKIALSNFESGFQRLSQANFLAAVPKNYMVEYARLCERKPNEVCTSWEARLAPLL
                     KNTIEAKVFERVATYKIPSIERTVSIPYKVDLDLQMFQKGYLFYLAGKFDDAYATWRN
                     LLKDYPRTSIKLRTKFWMGRAAQRSNHDSHAETLYKEVIRELPFAYYALLASWFGNID
                     LSRMMDLELPPVTKDAPVLSPSDIVHLRRAEQLLATGTADLAAIELSQVRARDKMSNE
                     FLIYLALLNHMAQNHLSTFQIFSELSSRGSQGLFSSYGQRLLFPTTYLPRIRPIASEV
                     KLDPLFVISLIKQESAFATQITSRANAFGLMQIILPTARDMDPRVEVIDLFDPDKNMK
                     LGTKYLRMLMNRYGGNAIHALSAYNAGPGNSDRWIREAPSDLPPEEYIELIGYRETRE
                     YVQNIIRNYYWYTRRVKSDLFPNLPALLLSLSTTTSPPTSKSSAELQNVISIKKRAPA
                     ARRQGRTFKR"
     gene            complement(3359..>3863)
                     /locus_tag="PLH57_04475"
     CDS             complement(3359..>3863)
                     /locus_tag="PLH57_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRK01897.1"
                     /translation="PVLYVANLDENGIKNPEAVPYFQAVKKIAESEGSQVVPICGALE
                     SEIASLESEEERAEFMKELGVSETGLDRLIRSGYALLGLITYFTAGEKEVRAWTIQKG
                     FKAPQAAGVIHTDFERGFIRAETYHFNDLVQCKSEAGVREKGLMRLEGKEYVVKDGDI
                     MHFRFNV"
BASE COUNT         1054 a          956 c          838 g         1015 t
ORIGIN      
        1 gactgagcta ctcctgaata tgacgattaa gtcggacaat cttgttacga aagaatacct
       61 ggttttgcaa gaattttacc ctttggaact ggcaggtgcc aaaggctcag gtcggtacag
      121 cggctgtttt aagacctcat ccatttttga acgaaccgtt gccattaggg tttcgatatc
      181 acccacccca agcccggcgg tcgttacggg ctctaatacc tgtatatgaa cgtcgtcttt
      241 gtggttaaac caatgtttgt cgtaatcgaa ataccggtgt aggtgctggt gcacaatcgg
      301 cactatcgga atttgggcgc gcacggccag gtgaaaggcc cccttcttaa aaggaagcat
      361 ttccgcgcta ccgcggttac gagtgccttc tggaaagatc caaaccgaca ggccacgctg
      421 gcgaacggcc ttagccgcat cgtcaaaacc gcttaaagaa tgggttcgat tttttcggtc
      481 gatcatcacg ttgcctgcgc ctgcaaacaa ccaaccaaaa agcggcagcc acttaagttc
      541 ttttttgcca atcaccaccg tgtcgggcgg gtaaatagtc gacatcgtaa aaatgtctaa
      601 attactttga tgattagcca cgtacacgca agggcgctgg tccatgcgct ccgggttgtg
      661 aaccatgact tttaatccga atattcgcaa accgacagta tgcagcaatt ttgcgaatac
      721 gtgccctacg gttgggtttc gccagcgaaa cggcaaaagc aacatggcca ttagtgaggc
      781 cactaaaaac caaactatgg ccgccaggat gcgccaaatc atgtctatcg tattcaaaaa
      841 agggatcttt ttacccctct caagaccgcc actcgagagt gttatgaaac tcatggtgtg
      901 cccatatcac gactcacggg ttgctgctaa gcggcaatcg aagttgtttg gacgagattg
      961 ctttgacaac attttagatg cgccttacca ttgaaccata tgccgtataa acgttcgaaa
     1021 tttctcatca ataggcgatt tcagctcaaa tttgcatttt tcgtttgttc ctggattttc
     1081 gctctcagca tggtttatcc gatcattatt tataatgttt tcgaatattt tcttaaaatt
     1141 ctcagcgcac cgcacgatgt attaaccgtc gacaaagtta agtacgtcga aaaccaggtt
     1201 gtcatcgtac tcggcatatt acagcttttg ttccttacga tcacattcat gctcagtatt
     1261 ttcttaagtc atcgtatcgc cgggccgttg tttaaattgc gccgcgcgat cgacgaagtt
     1321 tcgcgcggca attttgacca gcgcataacg tttcgaaaga acgaccattt tatggaactg
     1381 caagatggct tcaacgaaat ggtccaacat ctcggcgttc ggcgttggaa agaatagccg
     1441 cttaccggta gactaaacta acgaatggca aataactcga ttttcgcaat tctgagaatt
     1501 gctatcattt ctgcacattt aatttttctg gcaaccgccg gcttcgcggc cgatgaacca
     1561 atcggcgcaa atatctcgct catcgaacaa atcgaacaac ggattgtaac cgacaaagaa
     1621 aaaagaattc ccgggttact tgaagcttac gatttgctca gcaagggtaa aacggacctc
     1681 gcaatcaaca aacttcgact tctggccact aatcgcgagt tttcagattt ctatcactac
     1741 ctaatgggta gtgcccatta ccagaaaatg gtgcaagcag ccgaggcaaa aaagtggatc
     1801 accgccattt cctacggtga aacagcctcg attcatttca atagtgctca gacctcgttg
     1861 ccctacaata cgttcgaatc gcgaatcgcg ctttatctag gtttaactga agtttacctg
     1921 ggccaagcat acgccaaggc caaaaagaaa gatcaaaaaa ttgcgttatc taattttgaa
     1981 tcgggctttc agcgcctgtc gcaggcgaat tttctcgcgg cggttccgaa aaattatatg
     2041 gttgagtacg cgcgactttg tgaaagaaaa ccaaatgaag tgtgcactag ctgggaagct
     2101 cggctcgcgc ctcttctaaa aaatacgatc gaagctaaag tgtttgaacg cgtcgcaacc
     2161 tataaaatac cttcgatcga gcgaacggtc tcgattcctt ataaagtcga tctcgatcta
     2221 caaatgtttc aaaagggcta tttgttttac ttggccggaa aattcgacga cgcctacgcc
     2281 acctggcgta atttgttgaa agactatccg cgcacttcaa ttaaattacg cactaaattt
     2341 tggatgggcc gcgctgctca gcggtcaaat cacgattcac atgccgaaac cctatataaa
     2401 gaagtcattc gagaattacc attcgcctac tatgcattgc tggcgtcttg gtttggcaat
     2461 attgatttat cgaggatgat ggacctagag cttccccctg taaccaaaga cgcgcccgtg
     2521 ttgtcgccat cagatattgt gcacctacgc cgcgcggagc agctattagc aactggaacc
     2581 gctgatctcg cggccattga actctcgcaa gtaagagcga gagataagat gtcgaatgaa
     2641 tttctcattt acttagcgtt gctcaatcac atggctcaaa accatttgag cacgtttcag
     2701 atatttagcg aattatcatc acgagggtct caaggactgt tttcaagtta cggccagcga
     2761 ctactatttc cgaccaccta cttgccacga atccggccaa ttgccagtga ggtcaaactt
     2821 gacccactct tcgtaatttc tctcattaaa caagaaagtg cgtttgctac tcaaatcact
     2881 tcacgagcaa atgcctttgg attgatgcaa attattctgc ccaccgcgcg tgacatggat
     2941 ccgagggttg aggtgattga cctctttgat ccggacaaaa acatgaagct aggaaccaaa
     3001 tacctccgca tgctcatgaa tcgctacggc ggaaacgcga ttcacgcact ttcggcctac
     3061 aatgcggggc cgggcaatag cgatcgttgg attcgtgaag ctccaagcga tttgccaccc
     3121 gaggaataca tagagctcat tggttaccgt gagacacgcg aatacgttca aaacattatc
     3181 agaaattatt actggtacac ccgcagagta aaatcggatt tgtttccgaa cctaccagcg
     3241 cttctgttga gtctttcaac cacaacctca ccacctactt cgaaaagttc ggcggaatta
     3301 caaaacgtta tctctatcaa aaagagagcg ccagccgccc ggcgccaggg acgcactttt
     3361 aaacgttaaa tcgaaagtgc ataatgtcgc catctttgac gacgtattct ttgccttcca
     3421 aacgcataag gcctttttct cgaacccctg cttcggattt acactgaacc aaatcattga
     3481 aatggtaagt ttcggcccgt ataaagccgc gctcaaaatc agtgtgaatt accccggcag
     3541 cctgtggtgc tttaaatccc ttttgaattg tccaagcgcg aacttctttt tcgcccgcag
     3601 taaaataggt aattaggcct aagagcgcgt agcccgaacg aattaagcga tcaagtccgg
     3661 tttcggagac tcctaattcc ttcatgaatt cggcgcgctc ctcttcagat tcaagagaag
     3721 caatttccga ttccaatgcg ccacaaatag gcacaacttg cgacccttcg gattcagcga
     3781 tcttttttac agcttgaaaa taaggaactg cctcaggatt tttaatgccg ttttcgtcga
     3841 gatttgcgac gtaaagcacc ggt
//
LOCUS       DAOMXO010000160         3855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12925_length_3855_cov_5.417895, whole genome shotgun sequence.
ACCESSION   DAOMXO010000160 DAOMXO010000000
VERSION     DAOMXO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12925_length_3855_cov_5.417895"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="PLH57_04480"
     CDS             complement(<1..1464)
                     /locus_tag="PLH57_04480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK01898.1"
                     /translation="MQQKWLILSAVGCGTFMATLDSSIVNVALPSITTDFRTGLSFSR
                     WVVIAYLFSITGLLLFFGKMADIIGRKLIFKIGFVIFTFGSLICGLAETINQLILFRG
                     IQGFGAAMIMANGPAIITGAFPASERGKALGTLAMIVSAGLAIGPFLGGLFVKYFSWR
                     SIFFVNIPIGFLGTYLVHRYVPTAINPQESAMLEREARLPFGVRIQIYLEKLRHFDWL
                     GAILWMFAQLGYSLAIDQTNALGLGGPLQRIVSFSAAAMFFLFLIWESSIKDPVLDLN
                     LFRSRSFLAANISSLLNFIGISSVTLLMPFYFQNVRGLPPDQVGLLMTAIPATIFFVA
                     PISGRLSDKYGSRVLSAIGMLVLCSALAMLAIPGFGLQSDHPFAVVFYLTLVGAGIGL
                     FQAPNNNALMSSVAKQHLGVASALLATVRNFGLVTGAAMSTSLLMHFYGEQIAIHGVA
                     SRPVDNFTAALRFTFIAIAAFCAIGVLTSLLREDAKSN"
     gene            1543..2370
                     /locus_tag="PLH57_04485"
     CDS             1543..2370
                     /locus_tag="PLH57_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRK01899.1"
                     /translation="MAYESLREQAQKMRVSIIEMLTEAKSGHPGGSLSCTEIVAALYF
                     SEMRHDPQRPDWSERDRFVISKGHGVPAVYAAMAHAGYFPVQELMTLRKTGARLQGHP
                     DRRRLPGIEASTGSLGQGLSVAQGIALASKLSAKPWHTYCLLGDGEIQEGQVWEAAMS
                     APKFKLGSLTAILDYNHGQIDGRVDDVMSLEPLADKWRAFNWNVIEVDGHKFEDLIPA
                     LQKARKATDKPTFVIAHTIKGKGVSFMEGKIEWHGVAPNKDQETAAIKEINAKGVRS"
     gene            2367..3317
                     /locus_tag="PLH57_04490"
     CDS             2367..3317
                     /locus_tag="PLH57_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRK01900.1"
                     /translation="MSSNQKPKATRQAFGEVLAELGTKHTNLVVFDADLAKSTKSEMF
                     AKKFPDRFYEMGIAEANMIGTAAGMAMQGFVPFICSFGCFVTGRFDQIRMSVAYAGAN
                     VRVIGTHAGVGIGDDGHSQMGLEDLSLMRGLPTMTVLQPCDELETRQMIEFLMTHDAP
                     AYVRLTRQNLIPVHDGEYRFRLGRVDVLRESATANVAILATGATVQECVRAHEILQKQ
                     GVQITLANVPTIKPLNSDDILDIAKGHRWLLTVEDHYTIGGLGSAVAEIISDRSGPTR
                     LVRLGVNDQFGESGEPIELYDKFGISAAKIVQTVSGLLNS"
     gene            complement(3329..>3855)
                     /locus_tag="PLH57_04495"
     CDS             complement(3329..>3855)
                     /locus_tag="PLH57_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01901.1"
                     /translation="ATQAPKMAIITDFMIEDNKLTSLSLIELDGSQFTVRSMRARSQS
                     ETADESLIDQFVKTYKRVNAETYAELMANPYVVDMIIPAESFHTLANGKTLWSDKEIE
                     YDFEGRTLSGIIRSFSLTPEGTISQIRLEMISSTREGTKSKLPRTLRTLEYDVSKDRN
                     EIEAIESNFRYFEK"
BASE COUNT         1098 a          867 c          969 g          921 t
ORIGIN      
        1 attggacttc gcgtcttctc tcaataaaga ggttaacaca cctatggcgc aaaatgctgc
       61 aatggcgata aaggtgaaac gaagcgccgc agtaaaatta tcgaccggcc tcgaggccac
      121 accatgaatg gcaatttgtt ccccgtaaaa gtgcataagc aaactggtgc tcattgccgc
      181 acccgtgacc aatccaaagt ttcgaacagt tgcgagcagg gcagaagcaa cacccaggtg
      241 ttgttttgcc acagaactca ttaaagcgtt gttgttcgga gcctgaaaga gtccaattcc
      301 cgcgcccaca agagtaaggt aaaacaccac cgcgaacggg tggtcagact gcaggccaaa
      361 accaggaata gcaagcatcg ccaaggccga acagagcacg agcatgccaa tggcactcag
      421 tactcgagag ccgtatttgt cggataagcg gcccgatatt ggggctacga aaaaaatagt
      481 cgcgggaatg gccgtcatca ataggcccac ttgatccgga ggaaggcctc gaacgttttg
      541 aaagtaaaac ggcattagaa gtgtcacgct gctgatgccg ataaaattta gcaaactgct
      601 aatattggct gccagaaatg agcgagatct aaataaattc agatcaagca ccggatcttt
      661 aatgctagat tcccaaatca gaaagagaaa gaacatagcg gcagcgctaa acgacactat
      721 gcgctgcaac gggcctccca ggccgagcgc gtttgtttgg tcaatagcga gactgtaccc
      781 cagttgggcg aacatccata atattgcccc caaccaatcg aagtgtcgga gtttttccaa
      841 ataaatttga atgcgtacgc caaagggcag ccgagcctcg cgctcgagca tggcactttc
      901 ttggggattg atggcggtcg gaacgtaacg atgaactaaa tatgttccta agaaaccgat
      961 cgggatattg acgaaaaaaa tactacgcca agaaaaatat tttacgaata gaccacccaa
     1021 aaaaggtcca attgcgaggc ccgccgaaac gatcatggcg agggttccta gagcttttcc
     1081 ccgctccgag gccggaaagg caccggtaat gatagccgga ccattggcca taatcatggc
     1141 tgcgccaaaa ccttggatcc cgcgaaaaag tattaattga ttaattgttt cggccaatcc
     1201 gcaaattaga ctaccgaagg taaagattac gaatccaatt ttgaaaatta gttttcggcc
     1261 aataatgtcg gccatttttc cgaaaaaaag caggagccca gtaatactga atagatacgc
     1321 gattacgacc cagcgcgaaa aagacaggcc agttcgaaaa tcagtcgtaa tgctgggcag
     1381 cgcaacattg actatgctgg aatctaaagt ggccatgaac gtaccacaac ccacagccga
     1441 gagaatgagc cacttttgtt gcatcgttta acagtcctca tttcatttga gacgccatgg
     1501 ctatgtttgt caatcaacaa ccgcctgtgt tacccgagtg atatggcata cgaaagttta
     1561 cgtgaacagg ctcagaaaat gagagtttcg attatcgaaa tgctcaccga ggcgaaatcc
     1621 ggccacccgg gcggtagttt atcttgtacc gaaatcgtgg cggctcttta tttttccgaa
     1681 atgcggcacg atccgcagag gcccgattgg tccgagcgag atcgttttgt gatatcgaaa
     1741 ggtcacggag tgcccgcggt gtacgcggca atggcgcatg caggttattt tccagttcag
     1801 gaattaatga ctctgcgaaa gacgggagcg cgactacaag ggcaccccga ccgaagacgt
     1861 ttgccgggaa tcgaagcatc gacagggtct cttggccagg gactttcggt cgcacaaggc
     1921 atcgcgctag cgtcgaaact ttcagccaaa ccctggcaca cctattgttt attaggtgat
     1981 ggagaaattc aggaaggtca agtttgggag gccgcgatga gcgccccgaa attcaaactt
     2041 gggtccctga cggcaatatt agattataat catggtcaaa ttgacggacg agtagatgac
     2101 gtcatgagtc ttgaacctct ggctgataaa tggcgggcat ttaattggaa cgtgatcgaa
     2161 gtcgacggtc acaaatttga agatcttatt cctgcactcc agaaagcacg caaagccaca
     2221 gataaaccca ccttcgtaat tgcgcatact ataaagggga aaggtgtatc tttcatggag
     2281 ggcaaaattg aatggcacgg agtggcgccg aataaagatc aagagacagc ggccatcaaa
     2341 gagataaacg ctaagggagt acgatcatga gttcgaatca aaaacccaag gcaaccaggc
     2401 aggcgtttgg agaagtgctt gctgaattag gaacgaaaca taccaatctc gtcgtatttg
     2461 atgcagatct tgcgaaatct accaagtcgg aaatgttcgc caaaaagttc cctgatcgat
     2521 tttatgaaat gggaattgcg gaggccaata tgattggtac tgccgcagga atggcgatgc
     2581 agggatttgt accgtttatt tgcagctttg gttgtttcgt tacggggaga tttgatcaaa
     2641 tacgaatgtc agtagcctac gcgggcgcga atgtacgagt cattggaacc catgcgggtg
     2701 tcggcattgg agacgacgga cacagtcaga tgggacttga agacctttct ctaatgcggg
     2761 gtctaccgac catgacagtg ttgcaacctt gtgatgaact tgaaactagg cagatgatcg
     2821 agtttttaat gactcacgat gcaccggcgt acgtaagact tactcgacaa aatttaatcc
     2881 cagtacatga cggcgaatat cggtttcggc tcggaagagt agacgttctt cgcgagtcgg
     2941 caaccgccaa cgtggccatt ttagcgacgg gagccactgt tcaggaatgt gtgagagcgc
     3001 acgaaattct gcaaaaacag ggtgtgcaaa tcacgttagc aaatgttcct acgattaaac
     3061 ccctaaactc agacgacatt ctcgatattg caaaaggcca tcgttggctt ttaaccgttg
     3121 aagatcacta cacgattggt ggtttaggtt cagctgtggc ggaaattatt tcagatcgct
     3181 ccggtccgac gcgtttagtt cgcctcgggg taaatgacca attcggagaa tcaggcgagc
     3241 caatagaact ttatgataaa tttggaatat cagcggccaa gatcgttcag accgtcagtg
     3301 gacttttgaa ttcctgattt agcggatctt atttttcgaa ataccgaaag ttagactcaa
     3361 ttgcttctat ttcattgcga tctttagaaa cgtcgtattc tagtgttcgc aatgttcgag
     3421 gaagcttaga ttttgtgcct tcgcgcgtcg aacttatcat ttcgagtcga atctgagaga
     3481 ttgtgccttc aggcgttaaa ctaaaactgc gaataatacc actgagagtg cggccctcga
     3541 agtcatattc gatttctttg tcagaccaca gagtttttcc attggcgagt gtgtgaaagc
     3601 tttcagcagg aattatcatg tcaaccacgt aagggttggc catgagctcc gcgtaagttt
     3661 cggcgttcac gcgcttgtaa gtttttacaa actggtcgat taagctttca tctgcagttt
     3721 cgctctgaga acgtgcgcgc attgagcgaa ctgtgaattg gcttccgtcg agctctatca
     3781 atgacagcga agttaattta ttatcttcga tcataaagtc agtgataatt gccatttttg
     3841 gcgcctgggt cgccg
//
LOCUS       DAOMXO010000161         3853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12933_length_3853_cov_4.584518, whole genome shotgun sequence.
ACCESSION   DAOMXO010000161 DAOMXO010000000
VERSION     DAOMXO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12933_length_3853_cov_4.584518"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLH57_04500"
     CDS             complement(<1..447)
                     /locus_tag="PLH57_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01902.1"
                     /translation="MALLASTNLRADEDGGCQMIVGQALSQNPAVFAAPDARSLPRID
                     DLVERSNKFRAKLLRRNIRYREVPWALPETLESGIATASASGSAKSVVLKFFTSDAIE
                     AMRLRPLEERPVMFWVESVRQLFNFRRDPLLERLLDRVEQSPVRYLM"
     gene            complement(545..1636)
                     /locus_tag="PLH57_04505"
     CDS             complement(545..1636)
                     /locus_tag="PLH57_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HRK01903.1"
                     /translation="MTWGVVFLAFFGALRTAFSASPPIPYYIGPEVWEALDRKVAERD
                     CGEILTTISVYPVHPGHSSLRRVQNYAELSSDLILMNSRAVVLKDQGTVVESVNGIRE
                     RSLDVASLPRPGSNQLYRSRTRVFEHLNLFYHADRTVAYFERLGFETLGRPMMLYAAR
                     SLRSTAVYHRSVGVIHLLDGPIRDGFQSFLKDASVVAHEITHAVVDRTTINRNPKNLV
                     HAAMEEGYADYFAAAIQRHPAIAEYASGRNRNWIRDLRKRPVISHPEPHQIGHVFASE
                     LWRFRERAMQLEFSGIEIDRAVFRSIEVFNDADATFKGAVDLLVKALPKSLYGFQDEW
                     VRGFFSETLTLDDRYRGYLREGHLFRRTR"
     gene            1843..2163
                     /locus_tag="PLH57_04510"
     CDS             1843..2163
                     /locus_tag="PLH57_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01904.1"
                     /translation="MSDQTNKQTENVISLNSFRSLQSLREKAKPYRQRIEQMDKAGLL
                     IELIHYDELYKKNPNDVSFTIRAEQLMEVLEARAELTELRELARELHGKLRSRLHSQL
                     SQAK"
     gene            complement(2175..3491)
                     /locus_tag="PLH57_04515"
     CDS             complement(2175..3491)
                     /locus_tag="PLH57_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRK01905.1"
                     /translation="MRKAFVLDTNVLLFDPLAINKFGSNDVIIPITVIEEIDRFKREM
                     SENGRNARQFSRLMDQIRKQGQLSQGVKLPNGAILMVELGSGGPLPTELSADRADNRI
                     LATAMNMRKLAPKRPVVFVTKDTNLRIKADALGLDAEDYEPTHTHSEDLYSGVATLQV
                     PPEVVDEFYAQKKVQLKDHHLRANQYVIMKDSTNPHHTAMGRFNREIDCIVPLYKPAE
                     GLWGIFPKNAEQSFAIDMLLNDEIKLVSLVGKAGTGKTLLAIAAGLAKTVDEGIFHRL
                     LVSRPVFPLGKDIGFLPGDIEEKLNPWMQPIYDNIDFLFGASPVKARRGAGKGCQELM
                     NQGLLCIEPLTYIRGRSIPNQFMIVDEAQNLTPHEIKTIITRAGDATKIVLTGDCFQI
                     DNPYVDSNNNGLAYVVERFKDESISGHISLVKGERSKLSELASNLL"
     gene            complement(3488..>3853)
                     /locus_tag="PLH57_04520"
     CDS             complement(3488..>3853)
                     /locus_tag="PLH57_04520"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRK01906.1"
                     /translation="SPDAMRRLGDAPGMDILIKRKFPRAPYKVDVIAHDNKNVVEGQS
                     MVIGEGGLFLAADHVSHKVGSRLKIHFRESDLSAFNAVAEVVSVQRSPVSGYCLRFVA
                     ISDSDRKRISKFVTERSSR"
BASE COUNT          972 a         1022 c          914 g          945 t
ORIGIN      
        1 cattaaataa cgaacgggtg attgttctac tcgatccaaa agtcgctcta aaagcgggtc
       61 gcgtctaaaa ttaaaaagtt ggcgcacact ttcgacccaa aacattacgg gtcgctcttc
      121 gagtggacgc aaacgcatgg cctcaatcgc atcagaagtg aaaaatttta aaacaacact
      181 cttggcgcta ccactcgcgc tcgcagtagc gattcctgat tcgagcgtct ccggaagcgc
      241 ccacgggact tcacgatagc gaatattgcg ccttaataat tttgcgcgaa atttattgga
      301 acgctccact aggtcgtcta tccgcggcag agatctagcg tctggtgcag cgaataccgc
      361 aggattttgc gaaagcgcct gccctactat catctgacag ccgccatctt cgtctgcgcg
      421 aagattggtg ctcgccagca aagccaagct caatactaat aatggtaaaa ctgagaccct
      481 accccacact tttacacccc gggctacggc tatctcaagc cattgcacgt gtcaaaccga
      541 ggcgctaccg agtcctgcga aacaaatgac cctctctcaa gtaccctcgg taacggtcat
      601 ccagggtcag cgtttcacta aaaaatcccc gtacccattc gtcctgaaat ccgtagaggc
      661 ttttcggcaa cgcctttacc agcaaatcta ccgcgccttt aaaagtcgca tcagcgtcat
      721 tgaatacttc gatcgaccga aacaccgcac gatcaatttc gattccggaa aactctaact
      781 gcatggctcg ctcgcgaaag cgccaaagct cactcgcaaa cacatggcca atttgatgcg
      841 gctccgggtg cgatatgacc ggccgtttac gcaggtcgcg aatccagttt cgatttctgc
      901 ccgaggcgta ttcggcaatg gccgggtgcc gttggatagc tgcggcgaaa taatctgcgt
      961 agccttcctc catagctgca tgaactaaat ttttgggatt tcgattgatc gtggttcgat
     1021 caacaacggc gtgagtgatt tcgtgagcca ctacgcttgc gtcttttaaa aacgattgaa
     1081 atccgtcacg aatcggtcca tcgagtagat gaatcactcc cacactacga tggtaaaccg
     1141 cagtcgatct cagtgaacgg gccgcgtaca acatcatagg ccggccaagt gtctcgaacc
     1201 cgaggcgctc gaaataggcg acagttcggt ctgcgtggta aaacagattc agatgttcga
     1261 acactcgcgt tcgacttcga tatagttgat tggaaccggg gcgcggaaga ctagctacat
     1321 ccagacttct ctctcttatt ccgtttactg attcaacgac ggttccttgg tctttcaata
     1381 caactgccct tgaattcatg aggataagat ccgacgaaag ttccgcataa ttctgcacac
     1441 gccgcagcga cgagtgcccc gggtggacag ggtagaccga aattgttgtt aggatttcgc
     1501 cacagtcccg ttctgcaacc ttgcgatcaa gagcttccca gacttcgggg ccaatataat
     1561 atggaatggg gggagacgct gaaaatgcgg ttctgagagc gccgaaaaac gcgaggaaaa
     1621 ccactcccca cgtcatcgct ccgcgcgcaa atcccctgaa caataccccg ttgtccacga
     1681 tcaatttccc ccaggggtcc agtaacctct tgctcaggtg tttgacaata cttgtaagat
     1741 ttacatgctt cgggatttga aaaacgcgct cgtcaactag ggttcattgc ggcaccaaac
     1801 ttgctttggt accagccgga gtaccggggg agaacctgtt ccatgtcgga tcaaactaac
     1861 aaacagaccg aaaacgttat cagcctcaat agctttcgtt cgctgcaaag tttgcgcgaa
     1921 aaggccaagc cctatcgcca gcgcattgaa caaatggaca aggccggcct tttgatcgaa
     1981 cttatacatt acgacgaact ttataagaaa aacccgaacg acgtgtcgtt cacgattcgc
     2041 gccgagcagc taatggaagt gcttgaagct cgagcagagt tgaccgaact gcgtgagctt
     2101 gcgcgtgaac tgcacggcaa attgcgctcc cgccttcaca gccaactctc ccaagcaaaa
     2161 taaattcgac ttctttagag caagttgctc gccagctctg agagtttact tcgctcgccc
     2221 ttgacgagtg aaatatgccc ggagatcgat tcgtctttga atcgctcgac cacatacgcg
     2281 agcccgttgt tgttggaatc tacgtacggg ttatcgattt gaaaacagtc ccccgttagg
     2341 acaattttgg tagcatcgcc ggctcgggtg atgattgttt tgatttcatg cggagttaaa
     2401 ttctgcgcct catctacaat catgaactgg ttcggtatgc tgcggccacg aatgtaggta
     2461 agcggttcaa tgcacaaaag cccttgattc atgagctctt ggcagccttt acccgcccct
     2521 cttcgcgcct tcaccggact ggcgccaaaa agaaaatcga tgttgtcgta gattggctgc
     2581 atccaagggt tcaatttttc ctcgatatca cccggtaaaa atccgatatc tttgccaagc
     2641 ggaaacaccg gacgactgac gagcaagcga tgaaaaatac cttcgtcgac tgttttcgca
     2701 agtcctgccg cgatggcgag caacgtttta cccgtacccg ctttgccgac aaggcttacg
     2761 agtttgatct cgtcgtttaa aagcatgtcg atggcaaatg actgctctgc gtttttcgga
     2821 aaaattcccc acaatccctc agctggtttg tagagcggca caatacagtc gatctcgcga
     2881 ttgaaccgcc ccatcgccgt gtgatggggg ttggtggaat ctttcatgat cacgtactgg
     2941 ttcgcacgca agtgatggtc tttgagttgc accttttttt gggcgtaaaa ctcgtcaact
     3001 acttccggcg gaacttgcaa ggtcgcgacc cccgaataaa ggtcttcgga gtgagtgtgc
     3061 gttggctcgt aatcttcggc atcgagtcca agcgcgtcgg ccttaattcg taaattagtg
     3121 tctttggtta cgaatacgac cggtcgcttt ggtgcgagtt ttcgcatatt catcgcggtt
     3181 gcgagaatgc gattatctgc acgatcggcc gagagttcgg ttggtagcgg accgcccgaa
     3241 ccaagttcga ccatgagtat tgccccgttc ggcagtttta caccttggga gagctggccc
     3301 tgttttcgaa tttggtccat caaccgagaa aactgccgcg cattccggcc gttttcactc
     3361 atttcgcgtt tgaatcgatc aatttcctcg atcacggtga tcggaatgat cacatcgttt
     3421 gaaccaaatt tgttgatcgc caaagggtcg aacaaaagaa cattggtgtc gagtacgaaa
     3481 gcttttctca acgagagctc ctttcagtga cgaacttgga aatacgctta cgatcagaat
     3541 cagagatcgc tacaaatcgc agacaatagc cggacacggg agaacgttgc accgaaacca
     3601 cttcagccac tgcattaaag gctgaaaggt ccgactcacg aaaatgaatt ttcaagcggg
     3661 atcctacttt atgcgacacg tggtcggccg ctaaaaataa gccaccctct ccaatcacca
     3721 tggattgacc ctcaacaacg tttttattgt cgtgggcaat gacatccact ttatagggcg
     3781 ctcttggaaa ctttcgttta attaaaatat ccataccagg cgcatcgcct agtctgcgca
     3841 ttgcatctgg cga
//
LOCUS       DAOMXO010000162         3843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_12986_length_3843_cov_4.981785, whole genome shotgun sequence.
ACCESSION   DAOMXO010000162 DAOMXO010000000
VERSION     DAOMXO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12986_length_3843_cov_4.981785"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLH57_04525"
     CDS             complement(<1..719)
                     /locus_tag="PLH57_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK01907.1"
                     /translation="MKNFKSIRCVALAFLAIIVSAGFGCTKKETLNGKVYLTVSRDNI
                     KSADPTNAYDEVTLDVLQQIYETPYQYNYFADETEVIPLLADGMPEVSKDKLTYTIRL
                     KKGIRFQDDPCFKASGGKGRELKAQDLVYGWKRHADPATESQGFWIWEGKIVGIDEFA
                     AKFGKGRPAAEIMQDEVEGMKAIDDYTVQLKFKKPYPQLIYILTMTFTAPIPVEAITE
                     YGKGFPTHPVGTGPFRATQWQP"
     gene            856..1620
                     /locus_tag="PLH57_04530"
     CDS             856..1620
                     /locus_tag="PLH57_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01908.1"
                     /translation="MRYGIIAVFSILIAIDVGTFARGAIASDSCVADLGQILAQKHQS
                     KLEKKRNRTEQRLAQKEKSERIKRERSARENANIEGRITKMLDRSLNREPLLPSEVRE
                     MKAAARDLRIQGRVDDAKKLESRIARHEAKYHPPNRLRKLSSDEKQQLSEKVEISTYA
                     VKQVGTLNTDVREVFENKILPLIAEDPKNAGLNYRGANNKSKIDGIPVGAFKKVRFSL
                     KAEYRVIYHIDESGKAIIDAVGPRESVYSGKFSILD"
     gene            complement(1617..>3843)
                     /gene="alaS"
                     /locus_tag="PLH57_04535"
     CDS             complement(1617..>3843)
                     /gene="alaS"
                     /locus_tag="PLH57_04535"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRK01909.1"
                     /translation="WHKQEGVAKDRIFRFGEKDNFWSMGDTGPCGPCSEIFVDRGEKY
                     GCGKPACTVGCDCDRFMEIWNLVFMQFERSADGKMTALPKPSIDTGMGLERVTSVVQD
                     VATNYDTDLFQDLIASISKTTNSTYGKDQKTSVSLRVLADHCRAANFLIADGVLPSNE
                     GRGYVLRRIMRRAIRHGRNLGQTDAFFFKLTKPLIDVMGSAYPDLKDKREFLESTIKL
                     EEERFLITLDNGLRVLDEEIERSKSKSLSGEVAFKLYDTFGFPLDLTQVIAKERGFAV
                     DEAGFQTAMESQRSRSRQSWKGSGEEAVGEVYKSLHQKGIQTSFHGYQSLSHKGKVLA
                     IIEDGKLVKQSSTGDFQIVVDATPFYAESGGQVGDHGSIRGGNFEAKVVDAQKPTGDY
                     VVLHCVEARGSLKVDAQVEQTTEATIRSRTAKNHTATHMLHFALRKYLGPHVKQAGSL
                     VTPELLRFDFNHTKALSAEEIRKIEDYINESIERAETVGCEEMDKDKAMAKGAIAFFG
                     DKYGARVRVVTVGNYSTEFCGGTHVKNTSDIQMFKILNESGIAAGVRRIVAITGPSVL
                     SQLRQQERLLGELMHATKASAPNELLPKIEKLQLSEKELRKSLETQTRKSAASHVDDW
                     IRDAKTVGDGKLVFGIPSIPDGEAPAKILRDLADVLRSKLSKSVIALAAFDQEKNQSA
                     LLIAVTKDWTERFKAGELIKQVAPIIGGSGGGKPDLAQAGGKNNNFDAVLESLTKLLK
                     G"
BASE COUNT          989 a          915 c          927 g         1012 t
ORIGIN      
        1 ggttgccatt gggttgcgcg gaatgggccc gtaccgaccg gatgagtggg gaaccccttt
       61 ccatattcgg taattgcttc cacgggaatc ggtgcggtga aggtcatggt aagaatataa
      121 atcaactgcg ggtacggttt tttgaatttg agttgcaccg tataatcgtc gattgctttc
      181 atgccttcga cttcgtcctg catgatttca gcagcgggcc ggcccttgcc gaatttagct
      241 gcaaattcgt cgatgccgac aatcttgcct tcccagatcc aaaagccctg ggattcggtg
      301 gcaggatcgg cgtggcgttt ccacccgtag accaaatctt gggccttgag ctcgcgacct
      361 tttccgccgc tcgctttaaa acaggggtcg tcctgaaacc gaatgccttt ttttaaacgt
      421 attgtgtaag tgagtttgtc tttagaaacc tcgggcatcc cgtcggcgag cagaggaata
      481 acttcggttt cgtcggcaaa gtaattgtac tgataggggg tctcgtagat ctgttgaaga
      541 acgtcgagag ttacttcgtc ataggcgttt gtagggtctg cggatttaat gttatcgcgc
      601 gaaaccgtca ggtaaacctt gccgttgagg gtttcttttt tggtgcagcc aaagcccgca
      661 ctcacaatga ttgccaaaaa agcgagtgct acgcagcgga tagacttgaa gtttttcatt
      721 gggaaccccc tcttagattg tccagttaga gagtctaggc agccccgttg tgcgtcgtca
      781 atgatggaag gggcaattac tgccgaattt tccgtgctct aatctgagcc ttcgaagatt
      841 ccgaaccgta ttctgatgag gtacgggatt attgccgttt tcagcatttt gatcgcgatt
      901 gatgtgggta cgtttgcccg aggtgcaatt gcctccgact cctgcgttgc tgacttaggg
      961 cagattctcg cacaaaaaca ccaaagtaaa ttagagaaaa aaaggaatcg aactgaacag
     1021 cgattggcgc aaaaagaaaa gtctgaacga attaagcgcg aacgatctgc tcgagaaaac
     1081 gcaaacatag aaggtcgaat tactaaaatg ttggaccgtt cattgaatcg tgaaccatta
     1141 cttccgagtg aagtgcgcga aatgaaagcc gcagctcgcg atctccgaat acaaggtagg
     1201 gtggacgatg ccaaaaagct tgaatctcgg atcgcgcgtc atgaagcaaa ataccacccg
     1261 ccaaaccgat tgagaaaatt aagttcagac gaaaaacagc aactatcaga gaaagtagaa
     1321 atcagtacct acgccgtaaa gcaagtcggt actttaaata cagatgttcg agaagtgttt
     1381 gaaaataaga ttcttccatt gatcgccgag gatccgaaaa atgcgggatt gaattataga
     1441 ggtgccaata acaaatctaa aatcgacggg attccggttg gggccttcaa aaaagtaaga
     1501 ttttctctaa aagccgaata ccgggtgatt taccacatcg acgaaagcgg aaaagcaatt
     1561 atcgacgcag tcggtcctag agaaagcgtt tacagcggaa aattttcaat tctcgactaa
     1621 cccttgagaa gtttagtaag cgattccaaa acagcatcaa aattattgtt tttgccgccg
     1681 gcctgagcca ggtctggttt tccaccgccg gagcccccaa tgatcggagc gacttgtttg
     1741 atgagctcgc ccgctttaaa gcgttccgtc caatctttgg tgacggcgat caaaagtgca
     1801 gactgatttt tttcttggtc gaaagcagct agtgcgatga ctgatttact gagcttggaa
     1861 cgcaaaacat ctgccagatc ccgcaaaatt ttcgccggcg cttcaccgtc gggaatcgaa
     1921 ggaataccaa ataccaactt accatcaccc acagtttttg cgtcgcgaat ccagtcgtcg
     1981 acgtggctag ctgcactctt gcgtgtttga gtttctagcg attttcgcag ttctttttcg
     2041 gacagctgca atttttcgat cttaggcaac aactcgttcg gcgctgacgc tttcgtggcg
     2101 tgcatgagtt cgccaagaag cctttcttgt tgccgtaatt gcgataacac cgaggggcca
     2161 gtaattgcta cgattcggcg cacgccagca gcgatgccac tttcgttgag gatcttgaac
     2221 atttgaatgt ctgaagtgtt ttttacatgg gttccgccgc aaaactcagt tgaataattt
     2281 ccgaccgtga ccacacgtac acgagccccg tatttatcac cgaagaacgc aatagcgcct
     2341 ttggccatag ccttgtcttt gtccatttcc tcacagccga cagtttcggc gcgctcaatt
     2401 gactcattaa tgtaatcttc gatttttcgt atttcttcgg cacttagggc tttcgtgtga
     2461 ttgaaatcaa aacgcaataa ctcgggagtc actagcgagc ccgcttgctt aacgtggggg
     2521 cccaaatact ttcgcaacgc aaaatgaagc atgtgggttg cggtgtgatt tttggcagtg
     2581 cgcgatcgaa ttgtggcttc agttgtctgt tcaacttgag cgtctacttt taatgagccg
     2641 cgcgcttcca cacagtgaag caccacatag tctccagtag gtttttgcgc atcgaccacc
     2701 ttagcttcaa agttcccacc gcgaatcgaa ccatggtcac caacttgacc gccagactca
     2761 gcatagaaag gtgtagcatc cacaacgatt tgaaagtcac cagtcgaact ttgtttgacg
     2821 agtttaccat cttcgatgat cgcaagcact ttacccttgt gggataaaga ctgatagcca
     2881 tgaaaagagg tttgaatccc tttttgatgc aaacttttgt aaacctctcc gacggcttct
     2941 tcgccagagc ccttccagga ttgtcgcgac cgggaacgtt gagattccat agccgtttga
     3001 aaacctgctt catcaacagc aaacccgcgc tctttggcga tcacttgtgt gagatcgagc
     3061 ggaaaaccaa aagtgtcata gagtttaaaa gcgacctcac cggaaagaga tttgctcttg
     3121 gagcgttcaa tttcttcgtc gagcactcgt aatccgttgt cgagggtgat taaaaaacgt
     3181 tcttcctcaa gtttgatcgt actttctaag aattctcttt tgtctttaag atcaggataa
     3241 gcgcttccca tgacgtcgat cagcggcttg gtaagtttaa agaagaaagc atctgtttgc
     3301 ccaaggttgc gaccgtggcg aatcgcacga cgcataatac gccgcaaaac atagccgcgc
     3361 ccttcgttcg acggaagcac gccgtctgcg attaaaaagt tggctgcgcg acagtgatcg
     3421 gccagcaccc gaagtgaaac gctggttttc tgatcttttc cgtatgttga attggtggtt
     3481 tttgaaatcg aagcgataag atcctgaaac aagtcagtgt catagtttgt agcaacgtct
     3541 tgcaccaccg aggtcacacg ctccaggccc attccagtat cgatactcgg ctttgggagt
     3601 gcggtcattt ttccatcggc cgaacgttcg aattgcatga atacgaggtt ccaaatttcc
     3661 atgaaacgat cgcagtcgca acctacagtg caagcaggtt tgccgcagcc gtacttttca
     3721 cctcgatcga caaaaatttc agaacagggt ccacacgggc cggtgtcgcc catggaccaa
     3781 aaattgtctt tttcaccgaa acgaaaaata cggtcttttg cgacaccttc ctgtttgtgc
     3841 cag
//
LOCUS       DAOMXO010000163         3837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13021_length_3837_cov_4.355368, whole genome shotgun sequence.
ACCESSION   DAOMXO010000163 DAOMXO010000000
VERSION     DAOMXO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13021_length_3837_cov_4.355368"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2052)
                     /locus_tag="PLH57_04540"
     CDS             complement(<1..2052)
                     /locus_tag="PLH57_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01910.1"
                     /translation="MIIRRAAKYAILSVIVASILGVDSVNAARRAASPKLDKVESLAC
                     REVVKSERSRPTPPPIPQKPARPRVASAAKETIGNRNTDNRLTRQIEEALSVYRLDKL
                     FESARAQREEDFTKDAMFEDSRGSSSSPSRKQLLDYVVGKVRVAEITLKDGQTFTGHI
                     SEVATGLGGEKFVRIDEKVRFEFPTAGKGEVRYRVPNTSTRTVDAVQVRNIPLDQIEG
                     VRNLSPTAFWHENVSKEFSLQDYVKNGKMYDSAKDVAELERQSRAAGIFGSYSLEHQI
                     AKAENIREQARKDGVPSYAQKQLTDLAQVYVVEFEKTPLPGQSKGETVTGIVLSTFKA
                     PVSGKHTGKNFVRIDTNVRVDLNKMIKELENPDTTTAFYNRIESTFYAHPNRVAKVDA
                     PGVIDVPLADVQVATIRSVSVQEYLDRNFFGPKFDTADFLKFGRIFNSRIEAATVTHE
                     QVQEQLESAYATESEIDVFIHLAPALKYSGKVHGLFDNGPGDIRVEIGEHRGENAPAL
                     REIVLVTVTQKGNASRSSELPLERSSAYGENDVPLDVKEISSSKMLPISEIESKRTII
                     VAPEYAEEAENVPNAAITQDINPVKRPSTERGLPSVATGTQDLRKRHARAHSRADTVT
                     MSDDSIDSLNKIARKNPQEWERRTTVSGAIDGERLDRLWSRFEMTDNLKRASIRTPRG
                     EF"
     gene            complement(2142..2930)
                     /locus_tag="PLH57_04545"
     CDS             complement(2142..2930)
                     /locus_tag="PLH57_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01911.1"
                     /translation="MKGLSLPAATFHLAAFMVISSPFTTFANEKLTCNAQLNPRNELR
                     RAAIGTLAMVAVGSAVVYTNNQSEPKTERTSGTIVDRRWKHTVTIEAFEKTQKTAWLE
                     EVPNTPPKLPVNGKGEVSGAENIVCREELFRRDPCRLDHRESSCPEAIYREKCTFDTY
                     VWVFKESFTVPHSDRSLGPEVEPWWPTVTLGPLERPSFSKYYEVRILLDITDRQSNSP
                     VVTEPRLGTYEVASEEEFLKWVPGQKVEVDLTRFGQIKGLRKLK"
     gene            complement(3000..>3837)
                     /locus_tag="PLH57_04550"
     CDS             complement(3000..>3837)
                     /locus_tag="PLH57_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01912.1"
                     /translation="LPGARLRLFGTYIITTGVCFLLLYWWIVKNGYQPTWQAYFRDQV
                     LSSALEGRGGAQTGEPLYFVKILAQYYWPWMPFLLLGIWRSLKWLFTFEITDEAIYSW
                     IFGVFALGFIVGFSAVKWKFWYYIAPAYPALALFIASTMYSGVRKWAQAPLFRNIIIA
                     ASITWFVVGSILPIPLHRERVPEVQAFKETIRRSNVEGNVLFFGAPGDHNMVATSGDW
                     YFGKRVEKIDSISELARKLDSRKSAWLIVDNQFLERCGEEWCRRTIPIQAAHGTTLAL
                     YR"
BASE COUNT          957 a          885 c          947 g         1048 t
ORIGIN      
        1 aaactcgccg cgtggagttc taatcgaggc gcgttttaga ttgtccgtca tttcaaaccg
       61 actccagagc cggtcgagtc gttcgccgtc aatagcgccg ctaacagttg tgcgacgttc
      121 ccattcctgc gggttctttc gtgcgatctt gtttaaagaa tcaatcgagt catcggacat
      181 cgtgactgtg tccgcacggc tgtgagcacg agcatgtctt tttcggagat cctgagttcc
      241 cgtcgcgaca cttgggagac cacgttcagt gctgggacgc tttacggggt ttatgtcctg
      301 agtgattgct gcatttggaa cgttttctgc ttcttccgcg tattcaggtg cgacaataat
      361 tgtgcgttta ctctcgattt ccgaaatagg cagcattttt gagctggaaa tttcttttac
      421 gtctagaggt acgtcgtttt ctccataggc gcttgaacgc tcaagcggca attcggatga
      481 tcgcgaagcg ttgccttttt gagtcacggt aactagtact atttcacgta atgcgggtgc
      541 attttcacca cggtgttcgc cgatttcaac acgaatgtcg ccgggaccat tgtcaaacaa
      601 gccgtgtact ttgccagaat attttagtgc aggtgcgagg tgaatgaaca catcgatttc
      661 ggactctgtc gcataagccg actcgagttg ttcctgaact tgttcgtggg ttacggttgc
      721 tgcttcaata cgactattga aaatacgacc aaactttaga aagtcggcgg tatcgaattt
      781 ggggccgaaa aaattgcgat ctagatattc ttgtacgctg acagatcgaa tagtagcaac
      841 ttgtacatca gcaagaggaa catcgattac accgggggca tcgactttcg ccactcgatt
      901 gggatgtgca taaaacgtac tttcgatgcg gttgtagaac gcagttgtcg tgtcagggtt
      961 ctcaagttcc ttaatcattt tattgagatc tactcgaaca ttcgtatcga ttcgaacaaa
     1021 gttttttccg gtatgtttgc cagaaaccgg cgccttaaag gttgagagaa caatacctgt
     1081 aacagtttcc cctttagatt ggccagggag cggagttttc tcgaattcta cgacatagac
     1141 ttgtgctaaa tcagttaact gtttttgcgc ataggacggt actccgtctt ttcgagcttg
     1201 ctctcgaata ttctcagcct tagcaatttg gtgttccaaa ctgtaggatc cgaaaatgcc
     1261 agcagcccgc gattgacgct ccagttcggc cacgtctttg gcgctatcgt acatttttcc
     1321 atttttgaca tagtcttgaa gagaaaattc tttagaaaca ttttcatgcc aaaatgcagt
     1381 aggagataaa tttctaacgc cttcgatctg atctagtgga atgtttcgca cttgaactgc
     1441 gtcgaccgtt cgtgtggatg tatttggcac gcgatagcga acttcgcctt ttcctgcagt
     1501 gggaaattcg aatcgtactt tttcgtcgat gcgtacaaat ttttcgccgc ccaagccggt
     1561 tgcaacttca gaaatatggc cagtaaaggt ttgtccgtct tttaacgtga tctctgccac
     1621 gcgaactttg ccgacgacgt agtctaacaa ctgtttgcga cttggacttg aactggaacc
     1681 acgagagtct tcgaacattg catctttggt aaagtcttct tcacgctgag cgcgagccga
     1741 ctcaaagagt ttatctaaac gatagaccga caaagcttct tcgatctgcc tagtgagacg
     1801 attgtctgta ttccgattgc cgatggtttc tttggcggct gaggcaacac gaggtcgcgc
     1861 tggtttttga gggattggag ggggagttgg cctgctccgt tctgacttca ctacttcccg
     1921 gcaagcgagc gattcaactt tgtccagttt tggactagca gctcggcgag cagcgttgac
     1981 actgtcgact ccaagtatgg aggcgacgat gacacttagt attgcgtatt ttgcagcgcg
     2041 tcttatgatc atggagacgg agaataaagg aaagtaggac ggaatacaat acaaaaatag
     2101 tacgaaatta ccaagcgaag taacttattg aaaataatat gttattttaa ttttcgcaaa
     2161 cctttgattt ggccaaaccg cgttaagtct acttcgactt tttggccggg cacccatttt
     2221 aaaaactctt cttcggaagc gacttcgtaa gttccgagcc gtggctctgt gactaccggg
     2281 gaattcgact gacgatcggt aatatcgagt aaaattcgca cttcataata tttgctaaat
     2341 gaggggcgct cgagagggcc tagagttacg gttggccacc agggctcaac ttcgggaccg
     2401 aggcttcgat cggaatgagg tactgtgaac gactctttaa agacccatac gtaggtgtcg
     2461 aatgtgcact tttctcgata aatagcttcg gggcacgaag actctcggtg gtctaaacgg
     2521 catgggtcgc gacgaaaaag ttcttccctg caaactatgt tttcggcacc cgagacctcc
     2581 cctttgccgt ttacgggcaa cttcgggggt gtgttcggaa cctcctcgag ccacgcggtc
     2641 ttctgggtct tctcaaaggc ttctatagtc acggtgtgct tccaacggcg gtccacgata
     2701 gttcccgaag tgcgttcagt cttcggttcg gattggttgt tggtgtaaac gacagcacta
     2761 ccaaccgcca ccatggccag agttccaatc gcggctctgc ggagttcgtt gcgaggattt
     2821 agttgagcat tacacgtgag tttttcgttc gcaaaagtcg taaagggcga ggaaatcacc
     2881 ataaaagcag ctagatggaa tgttgcggct ggtaaactta ggcccttcat aagcgagtgc
     2941 ttcggtacca ttgtttggca ctatgcggca atgaataatc gctgagggac ccgaaaacct
     3001 tagcgataga gcgccagagt ggttccgtgg gccgcttgaa ttggtattgt tcggcgacac
     3061 cattcttcgc cgcacctttc gaggaactgg ttgtctacaa taagccaagc actctttcgg
     3121 gagtctagtt ttctagctaa ctccgagatt gaatcgatct tttcaactcg tttgccaaag
     3181 taccagtcgc cgctagttgc caccatattg tggtcgccag gggcaccaaa aaaaagaaca
     3241 ttaccttcaa cgttcgagcg gcgaatggtt tctttaaatg cttggacttc gggcactcgt
     3301 tcccgatgta gggggatcgg aagaatcgaa cccacgacaa accaggttat agaagctgca
     3361 ataatgatgt tccgaaataa tggagcttga gcccactttc ttacgccact gtacatcgtt
     3421 gaggcaataa agagggctaa tgccggataa gccggtgcga tatagtacca aaatttccat
     3481 ttaacagctg aaaacccgac aataaaacca agcgcaaaga ctccgaaaat ccaggaataa
     3541 atcgcctcgt cggtaatttc gaaagtaaaa agccatttta aactgcgcca aattcccagg
     3601 agtaaaaagg gcatccacgg ccaatagtat tgggccaaaa ttttaacaaa ataaagcggc
     3661 tcacctgttt gagcaccccc gcgcccttcg agtgcacttg agaggacttg atccctaaaa
     3721 taggcctgcc aggtgggttg atagccgttc tttacaatcc accagtagag aagcagaaag
     3781 cacacgccgg tggtaattat ataggttcca aataggcgaa ggcgagctcc aggtaat
//
LOCUS       DAOMXO010000164         3828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13055_length_3828_cov_5.874901, whole genome shotgun sequence.
ACCESSION   DAOMXO010000164 DAOMXO010000000
VERSION     DAOMXO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13055_length_3828_cov_5.874901"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1261
                     /locus_tag="PLH57_04555"
     CDS             227..1261
                     /locus_tag="PLH57_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRK01913.1"
                     /translation="MSKPRLVSSQTTQTKFRLAPELLVKIHDLMVKARTLEERLIKMY
                     KQNDGYFWIGGPGEESFNVPLGLLIKKGQGPKYDYFHGHYRSSAVLLAMGADPFDTIR
                     QMKNTANDPYSGGRNFVNHQSIQKWNVCPISSPIEVQYSMAPGTAIANKRAGGDGISI
                     VVGGDAGTAEGDFATCLVWSSRPVNELPLLICVTNNKWGISTSADTQHGDRCIADRGK
                     AFGMRTMTFNGLDPEESYLKLKEAMDYVRTERKPLLLEAQVSRLYGHSSASGANFIVN
                     EEDPLKTFEARLESGGILSKNEIQKIWDGYNEQMLNFARQAREEPQPDPESIWDHIYC
                     GQKGRVDRYW"
     gene            1274..2329
                     /locus_tag="PLH57_04560"
     CDS             1274..2329
                     /locus_tag="PLH57_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRK01914.1"
                     /translation="MANMAQAIRMALHYGEEHLGVTDIFGEDVGPPLGGVFTCTQGLK
                     TAWNSPLDERGIIGTGIGLALAGQKPVAEVQFCDYIFNTIDLLKIAGNTTWASNGDWC
                     VPLVVMTPVGSGIRGSIYHSHSFDAMMTHIPGWKIVMPSNPLDAYGLLLAAIKDPNPV
                     MYMKPKALLRVRGEELIPGEPTDEKKLREMIDAPIGDRSQWKARWPKLEEYTVEIGKG
                     KICREGSQLTVVGYGRMLPLCTQAAAELASDGIDAEVIDLRSLWPYDWEMISTSIRKT
                     RKVLFVNEDTEVTNFGEHLYRRATEEFFYELDAAPKLLAGAHVPGIGLADSLEMASVP
                     QLANIKEAIRALASEQP"
     gene            complement(2326..3570)
                     /locus_tag="PLH57_04565"
     CDS             complement(2326..3570)
                     /locus_tag="PLH57_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01915.1"
                     /translation="MSRKTTLRRTIGILGALAIGILPGCGDYSKSTREAREEFYAGEY
                     SDAARSLEKGAREEGVDQLLYVLDRATALHHAGSYEESNQEFHLADKLSEIKDYTSLS
                     AETATLITNDKIIPYKGEDFERVLISQYLALNYLMLGKKEDALVECRRVNHKLHMMIS
                     QGKRKYKLNPMAMYVAALLYEDAGEWSDAYVDYQQVYKLMPELSYLREDLYRLAAKNR
                     MPDHQRRWAEAFNLSVEEQKRILEEASRPELVMIFELGRGPEKQPHRAFPSIPQFVPR
                     YDSSNYADVFLEDGRSYRSHLLFDVEKTAIQNLEEKYAGLIAKRVGGFIAKEVIASQI
                     ERRTNNEWLGVLARLGMHAANQADLRSWLTLPKTFQILRVRISENELYKLRIVSPRGT
                     TQEKSVTIDRGEKRKILLVRSA"
     gene            complement(3577..>3828)
                     /locus_tag="PLH57_04570"
     CDS             complement(3577..>3828)
                     /locus_tag="PLH57_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HRK01916.1"
                     /translation="IDEEVTYHESGAVSGPTAKKRGQQTGADYILSGSLATNIQEVGE
                     DKLIYYKLTANLTSLETSTIDCTEEKQVRKKYKKRSTGL"
BASE COUNT          958 a         1002 c          912 g          956 t
ORIGIN      
        1 tcacgaatgt gggggcggag aagacgttta tcgttttcta agacccacga tttacctaaa
       61 aatccaactg agtgatttaa aagcggaact caaggaaact cccttgggca gcgcttcccg
      121 gggcgacatt gaacgcgaca ttgagcgctg caccgccact ctcaagctgt actagaccaa
      181 cgaatattta gttgtcgaac ccagccaagt cccctatctt ttttcaatgt ctaaacctcg
      241 actcgtatcg tcgcagacga cccaaacaaa gttccgctta gcaccagagc tgctcgtgaa
      301 aatccacgat ctcatggtca aggcccgcac gctcgaagag cgcctcatca aaatgtacaa
      361 acaaaacgat gggtattttt ggatcggtgg cccgggcgaa gaatcgttca acgtgcccct
      421 tggccttttg atcaaaaaag gccaaggccc aaagtatgac tactttcatg gtcattaccg
      481 ctcgagcgcg gtgctactgg ctatgggcgc cgacccgttc gacaccattc gccaaatgaa
      541 aaatacggca aacgacccct atagcggggg ccgcaatttc gtaaatcacc aaagcattca
      601 aaaatggaac gtgtgcccta tttcgtcgcc gattgaagtg caatactcta tggcgcctgg
      661 caccgcgatc gctaacaaac gtgcaggcgg cgacggtata tcgattgttg tcggcggcga
      721 cgccggcacc gcagaaggcg actttgccac ctgtttggtt tggagctcgc gcccagtcaa
      781 tgaactgccg cttctaattt gtgtgaccaa caacaaatgg ggcatttcta cttcagccga
      841 tacgcaacat ggcgaccgtt gcattgccga tcgaggtaag gcctttggca tgcgcaccat
      901 gacgttcaat ggcctggacc ccgaagaatc ttatctaaaa ctcaaagagg ccatggacta
      961 cgtacgcacc gaaagaaagc ctcttctact cgaagcccag gtttcacgcc tctacggcca
     1021 ctcttcggct tctggtgcga acttcatcgt caacgaagaa gacccactta agaccttcga
     1081 agctcgctta gagtcaggcg ggattctttc taaaaacgaa attcaaaaaa tttgggacgg
     1141 ctataacgaa caaatgttga acttcgcgcg ccaagctcgc gaagaacctc agcccgaccc
     1201 agaatcaatt tgggatcaca tttattgcgg ccaaaaaggg cgcgtagatc gttactggta
     1261 aaaggaacac acgatggcaa acatggcaca agccattcgc atggcgctcc attacggcga
     1321 agaacacttg ggtgtaactg atatctttgg tgaagacgtc ggcccaccac tgggtggagt
     1381 gttcacctgc actcaggggc ttaaaactgc ctggaactcg ccgctcgatg aacgcggcat
     1441 tatcggaacc ggaattggcc tcgcactagc tggccaaaaa ccagtggctg aagttcagtt
     1501 ctgcgattac attttcaaca ctatcgatct tctgaaaatc gcgggtaaca ccacttgggc
     1561 aagcaacggt gactggtgcg taccacttgt ggtcatgacg cccgtgggat ctggcatacg
     1621 cggatcgatt taccactccc actcgttcga cgcgatgatg acccacattc cgggttggaa
     1681 gatcgtcatg ccgtcgaatc cgctcgatgc ttacggcctt ttactagctg cgatcaaaga
     1741 ccctaacccc gtaatgtaca tgaagccaaa agcgcttttg cgggtgcggg gcgaagaact
     1801 aatccctggt gaaccgaccg acgagaaaaa gttacgcgaa atgattgatg cacctatcgg
     1861 cgatcgtagt cagtggaaag cgcgttggcc gaaactcgaa gaatacacag ttgaaatcgg
     1921 caaaggcaaa atctgtcgcg agggttcaca actcacagtc gtcggttacg gccgcatgtt
     1981 gccgctctgt acccaagccg cggccgagct cgcaagtgac ggcattgatg ccgaagtaat
     2041 cgacctgcgt tcgctctggc cctacgattg ggaaatgatt tcaaccagca ttcgcaaaac
     2101 acgcaaagtg cttttcgtga acgaagacac cgaggtcacg aattttggtg agcacttgta
     2161 tcggcgcgcg accgaagaat ttttctatga attagatgct gcgcctaaat tgcttgcggg
     2221 tgcccacgta cccggcattg gcttggcgga ttcgctggaa atggcttcgg ttccgcaatt
     2281 ggccaatatt aaagaagcca tacgcgcgct cgcatcagaa caaccctaag cgcttcgaac
     2341 caataatatc ttacgtttct cgccgcgatc gatggtgacg ctcttttctt gagtcgtgcc
     2401 acggggggag acgattcgca gtttgtatag ttcgttctct gaaattcgca ctcgtaggat
     2461 ttgaaaggtt ttgggcaagg tcaaccaact acgcagatcg gcctgattgg ccgcgtgcat
     2521 tcctagccgc gcgagcactc ctaaccactc gttgttggta cgccgctcga tttggcttgc
     2581 gatcacttcc ttagcaataa agccaccaac acgttttgct attaaaccgg catacttttc
     2641 ttctaaattt tgaatagcgg ttttttcgac atcgaacaac aaatgcgaac ggtacgatcg
     2701 cccgtcttcc agaaaaacat cggcgtagtt cgaactgtcg tatcttggca caaactgtgg
     2761 aatcgacgga aaggctcgat ggggctgttt ttctggacca cgtcctagtt caaaaatcat
     2821 cactaattcc gggcgactgg cttcttccaa aatgcgtttt tgttcttcga cggaaagatt
     2881 gaacgcctcg gcccagcgac gctggtgatc gggcatacga ttttttgccg ccaatcggta
     2941 tagatcttct cgcaaataac taagttctgg catcagtttg tatacttgct gataatccac
     3001 ataggcgtca ctccactcac ccgcatcttc gtacaaaagc gctgccacat acattgccat
     3061 cggattaagt ttatatttcc tcttaccctg agaaatcatc atgtgcagtt tgtggttcac
     3121 ccgtcggcac tcgactaatg cgtcttcttt ttttcccaac atcaagtaat tcaacgcaag
     3181 atattgcgaa attaacaccc gctcgaagtc ttcacccttg tagggaataa ttttatcgtt
     3241 agtgatcaga gtggcggttt cagccgaaag actggtgtaa tctttaattt cagaaagttt
     3301 atcggccaaa tgaaactctt gattgctctc ttcataactg cccgcgtgat gcagagcagt
     3361 ggcgcgatcg agtacgtata aaagctgatc gactccttct tcgcgagcac ctttttctaa
     3421 tgatcgggct gcgtcggagt attctcctgc ataaaactct tcgcgggcct cacgagtgga
     3481 cttagagtaa tctccgcagc ctggcagtat tccaattgcc agtgccccca aaattccaat
     3541 agtccggcgt agcgtcgtct tgcgactcat gccgccttag agtcccgtgg atcttttttt
     3601 gtatttcttg cgaacctgtt tttcttcggt gcaatcaatg gttgaagtct caagactcgt
     3661 aagattagcc gtgagtttgt agtaaattaa tttgtcttcg cccacttctt gtatgttggt
     3721 ggcgagtgaa ccactcaaaa tataatctgc accagtttgc tgaccgcgtt tcttggcagt
     3781 tggtcccgaa accgcaccac tttcatggta ggtgacttct tcgtcgat
//
LOCUS       DAOMXO010000165         3817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13119_length_3817_cov_4.970229, whole genome shotgun sequence.
ACCESSION   DAOMXO010000165 DAOMXO010000000
VERSION     DAOMXO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13119_length_3817_cov_4.970229"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLH57_04575"
     CDS             complement(<1..762)
                     /locus_tag="PLH57_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HRK01917.1"
                     /translation="MRHDSRIPNGLFYLRHFVSPRERADILAWIQTLHPIWEQRFSTR
                     RPPPEGEAQRWLLRPVYWLGNWQFACLDYYHPPKGTLHRCVRAEPYPPALAGVVQRIE
                     TFVRKMYKGRDLPPKWAANTCLINFYGDKIENGKRTDTARVGEHKDFELGPVASLSFG
                     ERAFFQFVESKARGTRERVVFEQWLVDSSLQVFGTPRWKDQTFHRVQRVERKQKTHFN
                     IGVPNFETRRINFTFRYVPEEHIVRLKDLPKEKWLD"
     gene            complement(770..1177)
                     /locus_tag="PLH57_04580"
     CDS             complement(770..1177)
                     /locus_tag="PLH57_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01918.1"
                     /translation="MEKRVNLVLWNLVLLDVVIATVALGFPEVWCWVFHGSDWPSFTD
                     PYGLLRRTGASWAAFAIFQTLAALRWKREPFWLVLVAGLRLGEIFTDWIYLGFASSAG
                     AMTWFGAAALFISPPANAWIGYYLVGCYRKLSR"
     gene            complement(1193..2089)
                     /locus_tag="PLH57_04585"
     CDS             complement(1193..2089)
                     /locus_tag="PLH57_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRK01919.1"
                     /translation="MSIDKINLGRQLYFDPRLSKDSTVSCNTCHNATASGTDNLRFSK
                     GVGGKEGGRNSPTVWNAAYLSVQFWDGRAPTLEEQAKGPLINPVEMAMENHDAVVARV
                     AAIKGYEAQFAKVFGGTQPVTIDRIAQAIAAYERTLVTPNSPFDRFIKGNKSAMSPAA
                     QRGWKTMQAVGCTSCHSGPVFAGPPLPTGTGFYQKFPLIPDEAIEAKYGFRKDLGRYD
                     VTKNEADKNMWRVPTLRNVELTAPYFHNGSVDSLEEAVRIMAKTQLNRQLQENEINDI
                     VAFLKSLTGKRPKEKAPVLPKS"
     gene            2226..2786
                     /locus_tag="PLH57_04590"
     CDS             2226..2786
                     /locus_tag="PLH57_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRK01920.1"
                     /translation="MIKKFQKLKLLLCLFGIFSGGFVMSAVTSIYETKIESIDGKPMA
                     LGEYKGKVLLIVNTASECGYTPQYKGLQALHERFSEKGLVVLGVPCNQFGGQEPGTEA
                     EIKKFCELKYRTTFPMLKKLDVKGAGQHPLYKELLAQSSDQTDVKWNFEKFLVGRDGK
                     VINRYRSKVEPESSELVQAIEKALQK"
     gene            complement(2788..>3817)
                     /locus_tag="PLH57_04595"
     CDS             complement(2788..>3817)
                     /locus_tag="PLH57_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01921.1"
                     /translation="YFALLVGAVTAKYRVDCPHKKGKISGMNKRSGWLRAILNFSLSI
                     AALNLTFNTSHGFDLRAFSTPPGQQTTSVRYAGTLKRDFHHQSLNVTAPLYKDDSNAV
                     LLSPRIVWFDLVPNNSAVPDLYDFQIGATYMRQTARQHVWSIQSTYGSASNKPFKDSS
                     VTTVSGLASYRFPTSSASTWSFFLQYSNNRPILNGWPLPGFAYTYVPSKDFRATFGVP
                     FASLYWKYADHWSVNLFTLAPWVARLQNNYIIGGPIQVFAGGELSQATFLLHGRADKK
                     ERFYYDEKKAFIGFQSPLSAALMGELEAGISDDRRFFSSKKYRPNPSPRTDLGASGYG
                     KLTLSARL"
BASE COUNT          960 a          899 c         1026 g          932 t
ORIGIN      
        1 gtcgagccat ttttctttgg gtaagtcttt gagtcgcacg atgtgttctt cgggcacata
       61 gcgaaacgta aagttgattc gacgcgtttc gaagttgggt actccgatat taaagtgagt
      121 tttctgtttg cgttccactc tctgcacacg gtgaaaggtt tgatccttcc agcgaggcgt
      181 gccaaacacc tgaagcgagc tgtcgactag ccactgctca aacacgactc gttcgcgggt
      241 gccgcgggcc ttagattcta caaattggaa aaatgctcgt tctccaaagc tgagcgacgc
      301 gacgggtccc aattcaaagt ctttgtgttc gccaacccga gccgtgtctg tgcgtttgcc
      361 gttttcgatt ttatcgccgt aaaaattaat caggcaggtg ttggcggccc atttcggtgg
      421 aagatctcgc cctttgtaca tttttcgaac aaaggtttcg attcgttgta cgacgccagc
      481 cagcgcgggt gggtagggtt ctgcacgcac gcaacgatgc aaggtgcctt tgggtggatg
      541 atagtaatca agacacgcaa actgccagtt gccgagccaa taaacggggc gcaataacca
      601 acgttgagct tcaccctcgg gaggaggtcg tcgcgtagaa aaacgctgtt cccagatcgg
      661 gtgtagggtt tgaatccaag ctagaatgtc tgctcgctcg cgcgggctca cgaaatgtcg
      721 aagataaaac aaaccattgg gaattctaga gtcgtgacgc aatttaaaac tacctactta
      781 gttttcgata acaaccgacg agatagtacc cgatccatgc attcgctggc ggcgagataa
      841 aaagcgcggc agcgccaaac caagtcatgg cgcccgccga actggcaaat cccaggtaaa
      901 tccagtcggt gaagatttct cctaaacgaa gtcccgcaac cagcacaagc caaaaaggct
      961 cacgcttcca acgaagagct gcgagcgttt gaaatattgc aaacgccgcc caactcgccc
     1021 cggttcgtcg aagtaagcca taggggtcgg taaaactagg ccagtcggaa ccgtgaaaca
     1081 cccaacacca aacttccgga aaccctaacg caactgttgc aatcacaacg tcgagtaata
     1141 caaggttcca gagtacgaga ttaacgcgtt tttccatggc gaagaattcg acctaagatt
     1201 tcggcagtac gggagctttt tcttttggac gtttgccagt caagcttttc agaaaagcga
     1261 caatatcatt tatttcattt tcttggagct gacgattcaa ttgagttttg gccatgattc
     1321 gaactgcctc ttcaagagag tccaccgaac cattgtgaaa gtaaggtgca gtcagttcga
     1381 cgtttcgtag tgtgggtacg cgccacatgt ttttatcggc ttcgttctta gttacgtcgt
     1441 agcgaccgag gtcttttcga aaaccgtatt tggcttcgat agcttcgtca gggatgagcg
     1501 ggaatttttg gtaaaacccc gtgccagtcg gtaatggtgg gcccgcaaag acagggccgc
     1561 tgtgacagct tgtgcagccg accgcttgca tggtcttcca accccgttga gcggcaggtg
     1621 acatagcgga cttattgccc ttgataaatc ggtcaaaagg actgttcggg gttaccagcg
     1681 tgcgttcata ggctgcaata gcttgagcga tgcgatcgat agttaccggc tgggtaccac
     1741 caaaaacttt ggcgaactgt gcttcatatc ctttgattgc ggcgacacgt gcaaccaccg
     1801 cgtcgtgatt ttccatggcc atttcaactg gatttatcaa aggccctttg gcttgttcct
     1861 cgagtgtggg ggctcggccg tcccagaact gcaccgaaag atatgctgcg ttccacacag
     1921 tgggcgaatt gcgaccgcct tcttttccgc caaccccttt tgagaaacgt aagttgtcgg
     1981 tgccgcttgc ggtggcattg tggcaagtgt tgcacgatac agtgctgtcc ttcgaaagac
     2041 gcggatcaaa atacagctga cgaccaagat ttattttgtc gatcgacatg gggttatccg
     2101 cggggatggg tggcttcgtg ggcagggctt cggtcgcccg actctgtgcg gcaaacccaa
     2161 cactaaggac cagaaacatt aaaaaccgag ctgtgcaaaa gatagtgttc gtcatagaaa
     2221 gaatcatgat caaaaaattt caaaaactga agcttctttt atgtttattt ggaatatttt
     2281 ccggaggttt tgttatgagc gcggtcacca gtatttatga gacgaagatt gaatccatcg
     2341 acggcaagcc catggcgttg ggcgaataca aaggcaaagt gcttttgatc gtcaacaccg
     2401 cctcagagtg tggttacacc ccacaatata aaggccttca ggcgctccat gaacggtttt
     2461 cggaaaaagg gctcgtggtc ttaggcgttc cctgcaatca gttcggcggt caggaacctg
     2521 gtaccgaagc cgaaatcaag aaattctgcg aactgaaata tcgcactact tttccgatgt
     2581 taaagaaact tgatgtcaaa ggcgcgggac aacacccact ctacaaagag cttctcgcgc
     2641 aatcgagcga tcagaccgac gtgaaatgga atttcgaaaa atttttagtt ggtcgtgacg
     2701 ggaaagttat caatcggtat cggtcaaagg tcgaacctga atcgagtgag ttggtacaag
     2761 ccatcgaaaa agcgttgcag aaataagcta taaccttgcc gatagggtga gtttgccata
     2821 gccactcgcg cccagatcgg tgcgtggcga cgggttcggt cgatattttt tggaagaaaa
     2881 aaagcgccgg tcgtcggata ttcccgcttc gagttcgccc atgagtgccg cggacaatgg
     2941 agattgaaag ccgatgaagg ccttcttttc gtcgtagtag aaacgttctt ttttgtcggc
     3001 tcgaccatgc aataaaaagg ttgcttgcga gagttctccg ccggcgaaaa cctgaatcgg
     3061 accaccgatg atatagttgt tttgaagtct cgcgacccag ggggcgaggg tgaataggtt
     3121 cacgctccag tggtcggcgt atttccaata cagcgaagcg aagggcaccc caaaagttgc
     3181 tcgaaaatcc ttagatggga cataggtgta agcaaagccc ggcagaggcc aaccgttcaa
     3241 aatggggcgg ttgttggagt attgcagaaa aaaactccag gtgctggcgc tgctggttgg
     3301 gaaccgatac gaagccaatc cactcacggt ggtgaccgaa gagtccttaa acggcttgtt
     3361 acttgccgat ccataagtag attggatcga ccagacgtgt tgccgagcgg tttgtcgcat
     3421 atatgttgcg ccgatttgga agtcgtagag atccggtact gcggagttgt tgggcacaag
     3481 gtcaaaccag actattcggg gcgacagcag cactgcgttt gaatcgtctt tatacagcgg
     3541 agcggtcacg ttgagggact ggtggtgaaa atctcgtttg agtgttcccg cgtagcgcac
     3601 cgaggtggtt tgttgtcccg gcggggtaga aaaggcccgt agatcgaagc catggctggt
     3661 attgaatgtt aggttcagtg ccgcaatcga gagtgaaaaa ttcaagattg ctcgcaacca
     3721 gccagagcgt ttgttcattc cagatatttt gcccttcttg tgggggcagt caacgcggta
     3781 tttcgcagtg actgccccca caagaagggc aaaatat
//
LOCUS       DAOMXO010000166         3815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13130_length_3815_cov_4.630053, whole genome shotgun sequence.
ACCESSION   DAOMXO010000166 DAOMXO010000000
VERSION     DAOMXO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13130_length_3815_cov_4.630053"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PLH57_04600"
     CDS             <1..995
                     /locus_tag="PLH57_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01922.1"
                     /translation="GLGLMTTVGLTGLSIASNLAWSNERSNPPGAEGGAMIPALDSRT
                     KEPLDLDKLDYDKDVKNAADHVSKWFWAEFGQTPPPGLLETMTSAEYEDILKTIAVYD
                     ETSARRLELERTANREILMKKAFLEPTSMVFPYEERMNGYARRFEYYMDAELMLKYDP
                     NQKMPLDPTAPELPHMISTATEARILAYRELQQAYVEMVKYSGWSPWLGKIAAYAHKV
                     SGGVKKILPKELGAAAGAGGLGYLAISGEQAEFSSQLAVFSSSAVVGGVAGYTCALLL
                     SEIPKGVVGWWIMPTINGWTAPRYARNVFRKAFSQSARAKEARIRLETFGKRKK"
     gene            complement(999..2261)
                     /locus_tag="PLH57_04605"
     CDS             complement(999..2261)
                     /locus_tag="PLH57_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRK01923.1"
                     /translation="MDNITHSLVGLACAEAITAIKPELRKYSRPILLASLIANNLPDL
                     DFAYTRITTSASGERIGQILHHRGHTHTLAFALPGAAILAALLRWLSGFKSLKSLNAP
                     FGVLFFAAALGISTHVFADSWNNYGVHPFWPFWNSWIYGDSLFIVEPLIWAIGLPFAY
                     FRFSSKFVRALIFTLLGGLLYLMVQYRATHWGSVALWLVSVVVSFKLWRNWFQGHRQQ
                     STAWVLVLLLIPVFFLRSFSISMDQRTRLWLRHAFGTTVLDVSLTPLPLNPFCWTGTS
                     VETDRTDYVARRFTVAFASWLVPASLCPSIFSSAKFTADLKPVTQSKALEFEKAGLFQ
                     WVGETRLPLERLRSFYDHNCEFSAVMKFYRVPFIKRQGAGQWIVGDLRYDRSEGRDFP
                     EMIINDPPGKCPRFVPNWTPPREDLLKR"
     gene            2333..3538
                     /locus_tag="PLH57_04610"
     CDS             2333..3538
                     /locus_tag="PLH57_04610"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK01924.1"
                     /translation="MPTPFRALKDKNFRGFFFAQLISMSGTWTQGIAQSWLVYKLTHS
                     GWWLGVIMFCNHFPAFVVSPIAGVFADRGERRHLLMVTQCVQITQALVLAGLVFTHLI
                     QPWHIAVLAFLLGIMEGIDITVRHTITPDLVQRKDLPGAIALQAVNNNLSRILGPTIA
                     GIVIGTVGDAWCFVVNAVSFFPMIFWLITVRLKRNTAGSPESTLKSALITGVAYVLKT
                     PFVFRLIALSTFVCIVLSMYTALLPIIAKSVLKGDAQTLAWLTGMLGLGSVGGALLFG
                     RTAAAEGQRYYLKRSVLTFGIGILGLALATRVEFASAAMFTIGFSQMGIFPRINLLLN
                     HTAIEQMRGRVMAVYTMTFMGTVPFGALISGWCADRVGAPMVTAVAGLIAIAGGGALY
                     WMERGEKIK"
     gene            complement(3579..>3815)
                     /locus_tag="PLH57_04615"
     CDS             complement(3579..>3815)
                     /locus_tag="PLH57_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01925.1"
                     /translation="RTESGSEAAQLDQLIARGWGAHPNYVFIPNGDSFIAKLNEAFRI
                     LEPNLPDRSLWIASQNPNERYATLGDLLANEAAE"
BASE COUNT          956 a          886 c         1019 g          954 t
ORIGIN      
        1 tgggcttggg gctcatgacg acagtaggtt taacaggtct gagcattgcg tcgaacctgg
       61 cgtggtcaaa cgagcggtcg aacccacctg gtgctgaagg cggggcaatg attcctgcgc
      121 tcgattcgcg caccaaggag ccgctcgact tagataagct cgactacgac aaagacgtaa
      181 aaaatgccgc tgaccatgtt tcaaaatggt tttgggcaga atttggccaa acaccaccgc
      241 ctggtctttt agaaaccatg acgagtgcgg aatacgaaga tattttaaaa accattgctg
      301 tttacgacga aacttctgcg agacgtcttg aacttgagcg caccgctaat cgcgaaattc
      361 taatgaagaa agcgtttctt gagccgactt caatggtgtt tccttacgaa gagcgtatga
      421 atggttacgc tcgtcgcttc gagtattata tggatgcaga gttaatgcta aaatatgatc
      481 cgaaccaaaa aatgcctttg gatcctacgg ctccggagtt gccacacatg atttccacgg
      541 cgaccgaggc tcgcatcctg gcctatcgcg aattgcagca agcatatgtg gaaatggtga
      601 agtactccgg ctggagtcct tggctaggta aaattgcggc gtatgcccac aaagtttcgg
      661 gtggagtgaa aaaaattcta cctaaagaac taggtgcggc agccggcgcc ggtggcttag
      721 gctatctagc aatatcaggt gaacaagcag aattttcttc gcagctcgcg gtgttttcga
      781 gtagcgccgt tgtaggcggc gttgctggct acacctgtgc attgttgctc tcggaaattc
      841 caaaaggtgt tgtgggctgg tggattatgc caaccatcaa cggctggacc gctcctcgat
      901 atgctagaaa tgtatttcga aaagcattta gccagtccgc gcgtgctaaa gaagcccgca
      961 ttcgtttaga aacttttggt aaacgaaaaa aatagtaatt agcgtttcaa taggtcttca
     1021 cggggtggtg tccagttcgg tacgaatctt ggacatttcc ccgggggatc gttgatgatc
     1081 atttccggaa aatctcggcc ttcacttcgg tcatatctca aatctcccac gatccattgg
     1141 cccgcacctt gcctcttgat gaacggtacc cgataaaact tcattaccgc ggaaaactcg
     1201 cagttatggt cgtagaaact gcgtaaccgc tcaagtggta ggcgcgtttc acccacccac
     1261 tgaaaaagcc ctgccttttc gaattccaac gccttagact gagtcacagg tttcagatcg
     1321 gcagtgaact ttgccgaact aaaaatgcta gggcacaaag acgcgggcac taaccaggat
     1381 gcgaaggcga cggtgaaacg acgtgctacg tagtcggtac ggtctgtttc gacgctcgtg
     1441 ccagtccaac aaaacggatt taacgggagc ggtgttaacg atacgtcgag aacggtcgtc
     1501 ccgaatgcgt ggcgcagcca gagtcgagtg cgctgatcca tagagattga aaaggaacgg
     1561 agaaaaaaaa ccgggatgag tagcaatacc aatacccaag cggtgctttg ttgtcggtgt
     1621 ccttgaaacc agtttcgcca aagtttgaac gaaacaacaa cggataccag ccaaagagca
     1681 accgaccccc agtgggtggc gcgatactgc accattagat acagcagtcc cccgagcagc
     1741 gtaaatatca gcgcccgcac aaatttgctg ctaaagcgaa aataagcaaa cggaagtcca
     1801 atcgcccata tcaggggttc gacaatgaaa agcgaatcgc cgtaaatcca agagttccaa
     1861 aagggccaaa atggatgcac gccgtagttg ttccaagagt cggcgaatac gtgagtgctg
     1921 atccccagtg ccgccgcaaa gaagagcacc ccaaaaggtg cgtttagtga tttaagcgat
     1981 ttaaagccag agagccaacg aagcagggct gctaatatag cagcgcccgg taaggcgaac
     2041 gcaagcgtgt gggtgtggcc gcggtggtga agaatttgac caattcgttc gccagaagcc
     2101 gaggtggtta ttcgagtgta ggcaaaatcg agatcgggca aattatttgc aattagagat
     2161 gcgagcagta tggggcggct gtattttctt agttccggtt taatcgcggt gatagcttcg
     2221 gcgcaagcaa gtccgacgag ggagtgggtg atgttgtcca ttctactggt atcctttttg
     2281 gttttcccct ggctcaggtg atctcgaatc aagtatactc gtagatcaga gtatgcccac
     2341 cccgtttcgt gctttgaaag acaaaaattt tcgcgggttc ttttttgccc aactcatttc
     2401 gatgagcggt acttggacac aaggaatcgc tcagagttgg ttagtgtata aactcacaca
     2461 ctccggttgg tggctgggtg taatcatgtt ttgcaatcac tttccggctt tcgtagttag
     2521 cccgatcgcc ggagtttttg cagaccgagg tgagcgacgc cacttattga tggtcactca
     2581 gtgtgttcaa atcacccagg cgctcgtgtt ggccggccta gtgttcacac atctaattca
     2641 gccttggcat atcgccgtac tggcctttct gctcggaatt atggaaggca tcgacatcac
     2701 agttcgtcac actatcactc ctgatctcgt ccaacgaaaa gatctacctg gggcaatcgc
     2761 gttacaggcc gtaaataata atttgtcgcg catattggga cccacgatcg ctggaattgt
     2821 aattggaacg gtgggcgacg cttggtgttt cgtcgtgaac gcggttagtt tttttccgat
     2881 gatattttgg cttattaccg ttcgattaaa acgcaatact gcgggttctc cggaatcgac
     2941 actaaagagt gccttaatca ctggggtggc ctacgttctt aaaacgccct ttgtttttag
     3001 attgatcgcc ttgtcgacgt ttgtctgcat cgtgctatct atgtacaccg cgcttcttcc
     3061 tattattgcg aaatcggttt taaagggtga tgcgcaaact cttgcctggc tcaccggtat
     3121 gttaggtctc ggttcggtcg gaggtgcgtt gttattcggg cgaacggccg cggccgaggg
     3181 gcagagatac tacttaaaac gttcagtttt aacattcgga ataggaattc tcggactcgc
     3241 gcttgcgact cgagtggaat ttgcttccgc ggcaatgttt acgattggct tcagccaaat
     3301 gggcatattt cccagaatta atttgttgtt aaaccatact gcaattgaac aaatgcgcgg
     3361 gcgagtgatg gccgtttaca ccatgacttt tatgggaacg gtgccgtttg gggcactgat
     3421 cagcgggtgg tgcgcagatc gtgtgggcgc tccgatggta actgccgtag cgggcttgat
     3481 agcgatcgcc ggtggcggtg cgctttattg gatggaacgg ggagaaaaga taaagtgaat
     3541 ctccgaatag ttctgagtgc agttttcggc tactaaactt actcagcagc ttcgtttgct
     3601 aacaaatcgc caagcgtcgc gtagcgttca ttcggatttt gggacgcaat ccaaagactg
     3661 cggtcaggga gattgggctc gaggatgcga aaggcttcgt tcaatttcgc aatgaaacta
     3721 tccccatttg gaataaaaac atagttagga tgggcgcccc aaccgcgagc aatgagctga
     3781 tcaagctgcg cagcttccga gcctgattcg gtacg
//
LOCUS       DAOMXO010000167         3807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13158_length_3807_cov_6.654851, whole genome shotgun sequence.
ACCESSION   DAOMXO010000167 DAOMXO010000000
VERSION     DAOMXO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13158_length_3807_cov_6.654851"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..2550
                     /locus_tag="PLH57_04620"
     CDS             247..2550
                     /locus_tag="PLH57_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRK01926.1"
                     /translation="MKTRFTKRSALTLLLIGAGVSLLAGCQPKLLRTLPAATADSSSS
                     GGGGGGGGGGGGGGGGSTFGTTASGLRGMQAALGDFDHDGDLDVAFVSQSADAVLLSE
                     GDGAGTFAAVATFALNQTPISVATADINRDGDLDLFVTTDTGTVEVLLGDGAGSFAAA
                     VSEAGGAVNAGIAIADFSRDGVVDVAITILDSNQVGLMAGSIAGTLGVIATYAVGTAP
                     TGIASGDLDHDGNPDVVVTNSADNNISVRLGDGAGGLGAETTFAVGTTPVGVALGDFD
                     RDGNLDVVVANSGATTVSVRTGDGAGSFGAEVTYAVGSTPVGVAVGDFNKDGDLDLAV
                     TNNASDTLSVLLGAAGATFGAATNTATSDYPLYPLVGDFDRNGEVDTAVIEGDTAPTL
                     VSGTDPGEVATFTLTGVPLACSSAQDSVGGDVDNDGDVDLVVACAGDGTVKVYDNNGS
                     GGFALSESLNVGSGAGSFPAGVELADLNQDGWLDIAMALRSDGEIHTWTNDGDGTFTF
                     RDSESAHVGAGGGNPWDVEVGDIDKDGDIDIMAVGNEFDNDTNFEVFDNDGTGMIAST
                     DSISHTGDNGRRMTIGDVDNDGDLDVVVGIRDEGDTLIALNDGAGGFPAAFTSVLDDA
                     VGNDVLQPRLADINRDGVLDIIQGNAAGAGLLDRELQLMFGVGDGTFGAVTNFDLNAG
                     GMVGHDAITGDFDSDGDLDVAVPLQGLNAVNFLSGDGAGAFAAIESEATCDDPRYGFA
                     ADLDRNGSIDIGIACQADNAANIYLSD"
     gene            complement(2547..3332)
                     /locus_tag="PLH57_04625"
     CDS             complement(2547..3332)
                     /locus_tag="PLH57_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRK01927.1"
                     /translation="MSQNISVKVKVWRQDGPQATGQFESFDLSGISTDMSFLEMLDTL
                     NEQLLKEGKRPVEFDSDCREGICGSCGCMINGMAHGPERGATVCQLHMRSFKNGQTIV
                     VEPWRATAFPVIKDLVVDRSAFDRIIAAGGYVSVKSGRHADANVMLVPKDTAEKAMDV
                     AQCIGCGACVAACPNASASLFTGAKITHLALLPQGKVEAARRVQRMTDQMDSEGFGGC
                     TNIGECLAACPKEIKMEAIVSMRREFIKGAFAEPPRQAGSDNA"
     gene            complement(3341..>3807)
                     /locus_tag="PLH57_04630"
     CDS             complement(3341..>3807)
                     /locus_tag="PLH57_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRK01928.1"
                     /translation="IKRLMTVNEKGKRTVNEFYKDLGKIMWDKVGMARNEAGLKSAIS
                     EIQKMRAEYGENLKIGGNSSELNKNLEMASRVGDFLELGELMARDALMRKESCGGHFR
                     EESQTPDGEADRDDANFSFVGAWEYKGANAEPALHKENLVFENVKPSQRSYK"
BASE COUNT          816 a         1026 c         1018 g          947 t
ORIGIN      
        1 gaacaaatga atcttcgttt aaaatcgggg gcttgcgcgg ttcaacttta cacctcaatg
       61 atcttcgaag gccccggctt ggtatccagg ctagtacgga cccttaacaa ttcctaagcc
      121 agcctaacaa tcggcataaa tagccgattt caaagcactt tatttttaat tcaattcttt
      181 gtaacccccg gggatgcagt aaaatagaac caaggttaat ggcccatttt gagggtggga
      241 ttctgaatga agacacgctt tacgaaacgc tctgcgctta ctctgcttct aattggtgcg
      301 ggcgtttcat tattagcagg ttgccaacca aagctactgc gcacgcttcc ggcagccacc
      361 gcggactcta gttccagtgg tggcggtggt ggcggtggtg gcggcggcgg cggcggtggc
      421 ggcggctcta ccttcggcac tacggcttcc ggccttcgcg gcatgcaagc cgctctaggt
      481 gacttcgacc acgacggcga tctagacgtg gcctttgtca gtcagtcggc cgatgcggtc
      541 ctactttcag aaggtgatgg cgctggcact tttgccgctg tcgcaacatt tgcgttaaac
      601 caaaccccca ttagtgtagc caccgccgac atcaatcgcg acggtgactt ggatcttttc
      661 gtaaccaccg acacgggtac cgtagaagtt ctcttgggtg acggcgcagg ttcattcgcc
      721 gctgctgttt ccgaagcggg cggcgccgtg aacgccggca ttgctattgc cgattttagc
      781 cgtgacggtg ttgtggacgt agcgattact attcttgata gcaaccaagt agggctcatg
      841 gccggctcaa tcgcaggtac gcttggagtg attgcgactt acgcggtggg gaccgcgccg
      901 actggaatag cttccggaga cctcgatcac gatggcaacc ccgatgtcgt cgtcaccaac
      961 tctgccgaca acaatatttc ggtgcgtctt ggcgacggtg cgggcggcct gggtgccgaa
     1021 acaacttttg ccgtaggaac tacgccagtt ggcgtagcac ttggtgattt cgatcgcgac
     1081 ggtaacctcg acgtggtggt tgcaaattct ggcgccacta cggtgtcggt tcgcacgggt
     1141 gacggtgcag gctcttttgg cgctgaagta acctatgcgg ttggtagcac ccccgttggc
     1201 gtcgccgttg gagacttcaa caaagacgga gatttggact tggccgttac aaataatgcc
     1261 tctgacactt tgagcgtctt gctaggtgcc gcgggagcca ctttcggtgc agcgaccaac
     1321 actgcaacca gcgattatcc gctgtaccca cttgtgggtg attttgatcg caacggtgaa
     1381 gtagatactg cggtcatcga aggcgacacc gccccaactc ttgtttcggg cactgaccca
     1441 ggcgaggtgg cgacattcac tttaactggc gttcctttag cttgcagcag cgctcaagac
     1501 tccgtcggtg gtgacgtcga caacgacggt gacgtcgatc tggtggtggc ctgcgcgggc
     1561 gacggaactg ttaaagttta cgacaacaac ggatcgggcg ggtttgcact atcggaatca
     1621 ctcaacgtag gatccggcgc gggctcgttc ccagcaggcg tcgaattagc cgacctcaac
     1681 caagacggct ggcttgatat cgccatggca ctgcgaagcg acggcgaaat ccacacgtgg
     1741 actaacgatg gtgacggcac attcactttt cgtgatagtg aatcagctca cgttggtgcc
     1801 ggcggtggta atccgtggga tgtcgaagtt ggagatatcg acaaagacgg cgacatcgac
     1861 atcatggccg tgggtaacga gtttgacaac gatacgaact ttgaggtttt cgacaacgac
     1921 ggaactggca tgatcgctag cacggattca atttcccaca ccggagacaa tggtcgacgt
     1981 atgacgattg gcgacgtcga caacgacgga gatctcgacg ttgtggtcgg tattcgtgac
     2041 gagggcgaca ctttaattgc actcaatgat ggagcgggtg gttttcccgc ggcctttact
     2101 tcggtgttag acgatgctgt tggcaacgac gtcctgcagc cgcgcttggc cgacattaac
     2161 cgcgacggtg ttcttgatat tattcaaggc aatgcagccg gtgccggcct gctagaccgc
     2221 gaacttcaat taatgtttgg tgtgggtgac ggtacgttcg gcgcagttac caatttcgat
     2281 cttaacgctg gcggcatggt cggacacgac gccattactg gggacttcga ttcagacggt
     2341 gatctcgacg tggccgtgcc gctccaaggt ttaaatgctg tgaactttct ttcgggtgac
     2401 ggagccggcg ctttcgcggc catcgaaagc gaagccacct gcgatgatcc gcgttacggt
     2461 tttgctgccg atctagatag aaatgggtct attgacatcg gtattgcctg ccaggctgac
     2521 aatgctgcaa acatttatct gtccgactaa gcgttgtcgc tacccgcttg ccgtggcggc
     2581 tcggcaaagg caccttttat aaactcgcgg cgcatcgaca cgatcgcttc catcttaatt
     2641 tctttaggac aagccgctag acattcccca atgttagtac agccaccgaa tccttcacta
     2701 tccatttgat cggtcatgcg ctgcactctg cgtgccgctt ccactttgcc ttgaggtaat
     2761 agcgccaagt gcgtaatttt agctccagtg aacagcgacg ctgaggcgtt cggacacgca
     2821 gcaacacaag cgccacaacc aatgcactgg gctacgtcca tcgctttttc ggcggtgtct
     2881 ttgggcacga gcatgacgtt ggcgtcggcg tggcgtccag acttcacaga cacgtagcca
     2941 ccagcggcga tgatccgatc gaatgccgaa cggtcaacga ccaagtcttt gatcaccgga
     3001 aaagcggtgg ctcgccaagg ttctaccacg atggtctgcc cgttcttgaa cgatcgcatg
     3061 tgcaactgac aaactgtggc gccacgctca ggtccgtgcg ccatgccgtt gatcatgcac
     3121 ccacacgaac cacaaatacc ttcgcgacag tcactatcga actcgactgg tcgcttgcct
     3181 tctttaagta actgttcatt gagcgtatca agcatttcca aaaacgacat atccgtcgat
     3241 attccggaaa gatcaaaact ctcaaattga ccagttgcct gcggcccatc ttggcgccaa
     3301 actttcactt ttactgaaat gttttgtgac atggcgactc ctatttgtaa cttcgctggg
     3361 aaggtttaac gttttcaaac accagatttt ctttgtgcaa tgcaggttcg gcgtttgcgc
     3421 ccttatattc ccacgcaccc acaaaactaa aattcgcgtc gtcgcgatcg gcttcgccat
     3481 cgggcgtctg actttcttca cgaaagtgtc cgccgcagga ttcttttcgc atcagcgcgt
     3541 cgcgcgccat caattcccct agctctaaga aatcgccaac acgactagcc atttcgaggt
     3601 tcttatttaa ctcggaagag ttgcctccga tttttaaatt ttcgccgtac tcggcccgca
     3661 ttttttgaat ttccgagatt gccgacttaa gaccagcttc attgcgggcc attccaactt
     3721 tgtcccacat gatttttcca agatctttgt aaaactcatt aacggtccgt ttgccttttt
     3781 cgtttacggt cataaggcgt ttgatct
//
LOCUS       DAOMXO010000168         3807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13161_length_3807_cov_4.725746, whole genome shotgun sequence.
ACCESSION   DAOMXO010000168 DAOMXO010000000
VERSION     DAOMXO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13161_length_3807_cov_4.725746"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLH57_04635"
     CDS             <1..410
                     /locus_tag="PLH57_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRK01929.1"
                     /translation="PGGTLGRKLLKRVEDIMIPKGQIGTGLEQDSVKLLSERMSRSNL
                     GLVAILSASGKIVGVVTDGDLRRAYQKGDLANLTAKQVMNTKPKSIQKGRLMTEAVRL
                     AEEYRITALMVTNENGDFEGAVNLHDLIREKVV"
     gene            415..1359
                     /locus_tag="PLH57_04640"
     CDS             415..1359
                     /locus_tag="PLH57_04640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK01930.1"
                     /translation="MAHSNGAILVTGGAGFIGTHLCRGLRASGFNVRVFDLKKPSHLV
                     EGVEYLTGDVRDRSALEKVMGGVDSVFHLAADVGVELCEKQPEQTYATNVQGTLNVLH
                     VLHQIKIRENRDVKLIFSGSAVVYGKLGESGKPLTENLNLAVPISFYGAQKLTAEHAI
                     QLYSKKIGIPAVCFRFFNVYGPGQDPKSPYSGVISIFIDRALQGLTLDIHGEGNQTRD
                     FVSVNDIVEACIKAVRLPAKGCDGVPINLGTGSKTTVLEVAEIIRRNSGNAASIRHLG
                     SRAGDILHSLASIQRANEILFWKPKVTLETGLSELLRL"
     gene            complement(1345..1914)
                     /locus_tag="PLH57_04645"
     CDS             complement(1345..1914)
                     /locus_tag="PLH57_04645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRK01931.1"
                     /translation="MSKTYFHPTAVVDSGATIGEGTQIWHFSHVRESAKIGKNCVLGQ
                     NVYVGPNTSLGNNVHVQNNVSIFDCVTLEDDVFCGPSMVFTNVINPRSHVPRKNEFKA
                     TLVKKGATIGANATIVCGVTLGEYCFVGAGSVVTKDVLPHALVVGVPAKKIGWVCTCG
                     VTLANASAVLECKECQARYRIDSGCLKSQ"
     gene            2023..2946
                     /locus_tag="PLH57_04650"
     CDS             2023..2946
                     /locus_tag="PLH57_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRK01932.1"
                     /translation="MMVKIAVTSSSFSQNDILRKELSTRFPNVVFNEKGRTLTREETA
                     EVLRTVDGAIIGVEKIDSSVLPKSDRLKILSKYGVGIDNIDFDACRVAGVEVYWKSGV
                     NAYSVAEQTIGMILMSCRALFARCQSLKRGEWVKNGGTQLSSKTLGIIGLGNVGQKLV
                     ELLRPFGTKIIANDIEDRSDFCRKYGIEQVELDRVFRDSDIVSLHVPLTALTRQLVNA
                     ERLSLMKSSSLLVNTSRGAVVDQNALKKALLANIIGAAALDVYEEEPPRDLEFLSLPN
                     LICTPHICGNSSESVLAMGRSAIYGLNQRFT"
     gene            3022..>3807
                     /locus_tag="PLH57_04655"
     CDS             3022..>3807
                     /locus_tag="PLH57_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01933.1"
                     /translation="MLYPGDDYSYFAYSTAVVFGEYPNFDKEAVDEFRVPPLSRLGPA
                     VLSLPFTLLGSIVDRVSDNPIVSSRTPENIRSSWTLFGAIFASGVAAWLAMFVMYRTL
                     QYFVSARASQWSVIAALLLPGSILYAYRRPVFSHVYELLIQTALVFIFVQVQTKSRWQ
                     KKDPGRKTLFFSIALCSLALMVRQNGVLFALLWPLLIFCVSRWKLDLTRKTIFRALIV
                     ISCSLGIFYLFKYLPEFFYRERYVRANAVFETNDFLWALRDPWF"
BASE COUNT         1004 a          778 c          934 g         1091 t
ORIGIN      
        1 acccgggggg aacgcttggt cgaaaactgc ttaagcgcgt tgaagacatt atgattccca
       61 aaggacaaat tggtactgga ctcgaacaag atagcgttaa acttctgtcc gaacgaatga
      121 gtcgcagtaa cctgggacta gtggcaatat taagtgctag cggaaaaata gtgggagttg
      181 tgaccgacgg agatttacgt cgggcatacc aaaaaggcga tttggcgaat ctaactgcca
      241 agcaagtaat gaatactaaa ccgaaatcaa ttcaaaaagg tcgattgatg accgaggcgg
      301 ttcgcctggc ggaagagtat cgtataactg cactcatggt aaccaatgaa aatggagatt
      361 ttgaaggtgc tgtcaatttg cacgatctca ttcgagagaa ggtagtttaa ggttatggcc
      421 cattccaacg gcgcaatctt ggttacaggc ggagcgggtt ttatcggaac gcatctgtgc
      481 cgcggactgc gagccagtgg ttttaatgta cgggtattcg atttgaaaaa accttcacat
      541 ttagttgaag gggttgagta tttaacggga gacgtgcgcg atcggtccgc tcttgaaaaa
      601 gtaatggggg gagtagattc ggtttttcac cttgcggcgg atgttggtgt cgagctttgt
      661 gaaaagcaac ctgaacaaac ttatgcgacc aatgtccagg gtaccctgaa tgtattgcac
      721 gtgcttcatc agattaaaat tcgggagaac cgagatgtta agctgatttt cagtgggtcg
      781 gctgtggtgt atggaaaatt aggcgaatcg ggcaaacccc ttacagaaaa tttgaatctg
      841 gctgttccga tttcgtttta cggcgctcaa aagttaacag cggaacacgc gatccagtta
      901 tattcaaaaa aaattggtat tcccgcggtg tgtttcaggt ttttcaatgt ttatggaccg
      961 ggtcaggatc caaaatcacc ctattcggga gtaatttcga tattcatcga tcgagcgctc
     1021 caaggtttaa ctttggacat tcatggagaa ggcaatcaga ctcgtgattt tgtttccgtg
     1081 aatgacatcg ttgaggcatg cataaaagca gttcggttac cggcaaaagg gtgcgacggg
     1141 gttcccatta atcttggaac cggttctaaa accaccgtac tcgaagttgc tgaaattatt
     1201 cgtagaaatt ctggcaatgc tgcatcaatt cgtcatcttg gatcccgcgc gggagatatt
     1261 ttgcactcac ttgcttcgat ccaaagggct aacgaaatat tgttttggaa acctaaagta
     1321 acgttagaaa ctggtctttc ggaattattg cgactttaga caaccgctat caatacggta
     1381 gcgagcctgg cattctttgc attccagcac agccgaggca ttggcaagag tgaccccgca
     1441 ggtacatacc caaccaattt tttttgcggg gaccccaacc actagggcgt gaggcaatac
     1501 gtctttagtc accacacttc cagcgcctac gaaacagtat tcacccaatg ttacgccaca
     1561 aacgatagtc gcgttggctc cgatcgtagc cccttttttt acaagagtag ctttaaattc
     1621 gttcttgcgg ggcacgtgac tgcgaggatt aataacgttc gtaaatacca tcgaaggacc
     1681 acaaaaaaca tcgtcctcaa gggtcacgca atcaaatatt gaaacgttgt tttgaacgtg
     1741 aacattattg ccaaggctag tgtttggacc gacgtaaacg ttttgaccca aaacgcagtt
     1801 ttttccgatt tttgcgctct ctcgcacgtg agaaaaatgc catatttgag taccttcgcc
     1861 gattgtggcg ccggaatcga caacagcggt ggggtgaaaa taagttttac tcacagttgg
     1921 aatataggtg aagacaagca acttgaaaag cgggcaattt cgacaggatc tggtgcacac
     1981 ttgatccttg gcgggcctaa aaatgtctga gataacacaa atatgatggt taaaattgca
     2041 gtcacttcct cgtcattttc ccaaaatgat atcttaagaa aagagttgtc cacccgcttc
     2101 ccgaacgtag tttttaatga aaaagggcga actttaactc gcgaggagac ggccgaagta
     2161 cttcgaaccg tcgacggcgc cataatcggt gtcgaaaaaa ttgattcgag tgtgctgccg
     2221 aagtcggacc gccttaaaat tctttctaag tatggagtgg gtatcgacaa tattgatttt
     2281 gacgcatgcc gcgttgcagg ggtcgaagtt tactggaaat cgggtgtgaa tgcttattcg
     2341 gtggcggaac agacaattgg aatgattttg atgtcgtgca gggctttatt tgctcgctgc
     2401 caaagtttaa agcgaggaga gtgggttaaa aatggcggaa ctcagctgtc ctcaaaaact
     2461 cttggcatca ttggcttagg aaacgtcgga caaaaattag tggagctact tcgtccattt
     2521 ggaactaaaa ttatcgctaa cgatatcgaa gatcgatctg atttctgcag aaaatacgga
     2581 attgagcagg tcgaattgga tcgagtattt cgagattccg atattgtgtc tctgcacgta
     2641 ccgttgaccg ccctcactcg ccaattagtg aatgccgaac gactatcatt gatgaagtca
     2701 tcatcactat tagtaaacac ctctcggggc gcggtggttg atcagaatgc tttaaaaaaa
     2761 gcgcttttag ccaacataat tggcgccgca gcactagatg tatatgaaga agagcctccg
     2821 cgagatctag agtttctttc attgccgaat ctgatttgta ctccgcatat ttgtggaaac
     2881 tctagtgaat cagtgctcgc catggggcga tcggcaattt acggattaaa tcagcgattc
     2941 acctaaatgc gccaactgac aatagtatat tttcttttga tgtttttcct gtttcgcccc
     3001 ttccaattag agcgaccggg tttgttatat ccgggagacg attattctta ttttgcttat
     3061 tcgacagccg tggtttttgg cgaatatccc aattttgata aagaggcagt cgacgaattt
     3121 cgagtcccac cgctgagtcg gctgggcccg gcagtgctaa gtttgccatt tacattgctg
     3181 ggttcgattg tggaccgagt atcagataat ccgatcgtta gctcaagaac tcccgagaac
     3241 attcgttcga gttggacatt atttggggca atattcgcat ctggggtcgc agcgtggttg
     3301 gcgatgtttg tgatgtatcg cacgcttcag tatttcgttt ctgctagggc gtcgcagtgg
     3361 tcggttattg cggctcttct gttaccggga tcaatcttat acgcctatcg aagacctgtt
     3421 ttttcacatg tctacgaatt gttgattcaa acggcattgg tttttatctt tgttcaggtt
     3481 cagaccaaat cgcgctggca gaaaaaggat cctggacgaa aaaccttatt tttttcgatt
     3541 gcgctttgtt cccttgcttt aatggttcga caaaatgggg tgttatttgc attactttgg
     3601 ccgctactta ttttttgcgt gagtcgatgg aaattggatc ttactagaaa aacgattttt
     3661 cgagcgctca ttgttatttc ttgttctttg gggatttttt atttgttcaa atatcttccg
     3721 gaatttttct atcgcgaaag atatgtacgg gcgaatgcag ttttcgaaac caatgatttt
     3781 ctttgggcgt tgcgcgatcc ttggttt
//
LOCUS       DAOMXO010000169         3805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13174_length_3805_cov_5.954133, whole genome shotgun sequence.
ACCESSION   DAOMXO010000169 DAOMXO010000000
VERSION     DAOMXO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13174_length_3805_cov_5.954133"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /gene="pth"
                     /locus_tag="PLH57_04660"
     CDS             complement(<1..407)
                     /gene="pth"
                     /locus_tag="PLH57_04660"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRK01934.1"
                     /translation="MKLVIGLGNPGPEYVATRHNVGFMAVERLIDDLKGKFKTWGQQK
                     NALLADVSIDGERVLVLKPLTYMNLSGQAAVAAAHFYKILPEDICVLHDELDLPLGTV
                     RLKTGGGPGGHNGLRSLIASLATPNFSRVRMGVG"
     gene            complement(408..1352)
                     /locus_tag="PLH57_04665"
     CDS             complement(408..1352)
                     /locus_tag="PLH57_04665"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRK01935.1"
                     /translation="MRLCLFAGSSNRGLATKVAETLGTHVSDATIRRFADGEVFVEIE
                     ENVRGKDVYILQSTCPPVNDHIMELLIMIDALKRASTNSITAVIPYYGYSRQDRKVAP
                     RTPISAKLVADLLTAAGCSRVVGMDLHAGQIQGFFNIPFDNLYARPVILDHLKSLFSK
                     TDDLVFVAPDAGGVERARAFAKRINDAGVAMIDKRRTGPNVAKAMHLIGSVEGKIAII
                     LDDMIDTAGTLVEAAETVKKNGATKIYACATHAVLSDPATDRIKNSCLQQVIITDTIP
                     LSPAAQQVDKIHVLSSAGILAEAIRRIHSYESVSSLFT"
     gene            complement(1459..>3805)
                     /locus_tag="PLH57_04670"
     CDS             complement(1459..>3805)
                     /locus_tag="PLH57_04670"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRK01936.1"
                     /translation="NKGMSKVFVASNWYASDIEKLFSGGGYDPMANFVALGVSNSFID
                     SVRLKNNLVEIRQLVQIGFNRGFVLAPSYEVRYYLTPYLENPKYVAKETDKTFDRVGY
                     FEVPPTLEEESGRTSIKISKWDISKPVTFYVSANTPAEYVDAVKEGILYWNKAFGREV
                     LRAEVAPAGITAPDPSYNLIQWVPWDSAGFAYADALMDPRTGEIKNAQVYLTSAFAFG
                     SKARLRRFLRTYRPAAMARNDHRDHTDHQHGMLENAEEETPYSRQELLETAHPKNRTK
                     RPLYKGVALEFMRPARLCNYSLDEAAAANLEPLLEANADDATLLRVSQDYVRLVTAHE
                     VGHTLGLRHNFAGSLGASVTIPQRDAPFREYVLKNTVPNSEHVFGSSVMEYMHFMDDV
                     LAAAQIKSGGEALAYDKAAIQWGYSDQQMAEKSPLFCTDSHAMLGYLDCDTFDSSASP
                     IANWAYESVSSVAMLPTTLVERYMAAKAPHDPRDAIPLDAVALSPHVYGRNAAVRLFR
                     ALYPLSSEAYFIAIDRQHAVLDGLNMEETIAAQQLDYVKAQLKAIGGLEWAYFTLLPV
                     SKPYMSGVASIQGMSGRLSENLKKQLDAYLGRKDVRNFVGLDGKTHSFTDKEVAYIQS
                     RAGQFFDKFEAAYYAWAAIAFSAVHMDLEFKVLRDVPADGIMMGMKSALESTIRQTVL
                     TKSGKTRVGSVSVNGMRPTVTIPTYAYGTLERRAMANYLSSRILPIPNWSTATRQALA
                     SQMTSEIENSLGKRLAEVTEGSVSSDLYWWLMDERKVISSF"
BASE COUNT          950 a          966 c          924 g          965 t
ORIGIN      
        1 ccgaccccca tacgaaccct tgagaaatta ggggttgcaa gtgacgctat gagcgatcgt
       61 aaaccattgt gaccaccggg acccccgccg gtttttaagc gaacagtacc cagaggaagg
      121 tcgagctcgt cgtgtaaaac acagatatcc tctggcaaaa tcttataaaa atgggctgct
      181 gccaccgcgg cctgaccgct aagattcatg taagtgagcg gttttagtac cagaacgcgt
      241 tcaccatcga tcgagacatc cgccaatagg gcattttttt gttggcccca ggttttaaac
      301 tttcccttaa ggtcgtctat taggcgttca actgccataa atccgacatt gtggcgggtg
      361 gcgacgtatt cggggccagg atttccaagt ccgatgacga gcttcattta agtaaaaagc
      421 gaactcacgg attcgtaact gtgaatgcgc cgaattgctt cagctaaaat accggcactg
      481 gagagtacgt gaattttatc gacctgttgg gccgccgggc tcaaaggaat cgtgtcggtg
      541 atgattacct gttgaagaca tgagttttta attcgatcgg tggccggatc gcttaaaaca
      601 gcatgggtcg cacaggcgta aattttggtg gctccgtttt ttttcaccgt ttcagctgcc
      661 tcaaccaagg ttcccgctgt gtcgatcatg tcgtcgagaa ttattgcaat cttgccttca
      721 acactgccga taagatgcat cgctttggcc acattcggac ccgtgcgccg tttgtcgatc
      781 atcgcaacgc cggcatcgtt gatgcgtttg gcaaaggctc ttgctcgctc cacaccgccc
      841 gcatcaggtg caacaaatac aaggtcatcg gtcttactaa aaagagattt caaatggtct
      901 aggatcactg gtcttgcgta gagattgtcg aaaggaatgt taaagaaacc ctgaatctgc
      961 cccgcgtgca ggtccatacc gacaacccgc gagcaacccg cagcggttaa taagtcagcc
     1021 accagtttgg cgctgatcgg cgtgcgaggc gccactttgc ggtcttgtct agaatatcca
     1081 tagtagggaa tcaccgccgt gatactgttg gtagacgcgc gcttgagcgc gtcgatcatg
     1141 atcaacagct ccatgatgtg gtcattcacc ggtggacaag ttgactgtaa aatatagacg
     1201 tctttgcccc gaacgttttc ttcgatttcg acaaacactt caccgtcggc gaatcttcgt
     1261 attgtggcat cgctcacgtg ggtaccaagc gtttcggcaa ctttagtagc tagacctcga
     1321 ttggatgaac cagcaaaaag acacaaacgc atggggtggg gaacctccgt ctgcagtatt
     1381 tttaccaaca aaaaacaaag gccgaaacca cgaaagtggc tccggccctt tttattttta
     1441 ttatctggct ggggttgctt agaacgaaga gatcactttt cgttcatcca tcaaccacca
     1501 ataaaggtcg ctcgaaacac ttccctcggt gacttctgcc aaacgtttac ccagcgagtt
     1561 ttcaatctcg gaggtcattt gagaagccag agcttgacga gttgcggtag accagttcgg
     1621 tattggcaaa attcttgaac tcagataatt cgccatcgcc ctgcgttcaa gagtcccgta
     1681 ggcataggtc ggaatagtta ccgttggacg cattccattc acggaaactg aacctactcg
     1741 tgttttcccg ctcttagtta gaacagtctg acgaatagtt gattccaacg cactcttcat
     1801 tcccatcata atgccatcgg caggaacatc gcgtagaacc ttaaattcga ggtccatgtg
     1861 aacagcgcta aacgcgatag cagcccatgc atagtaggcc gcttcgaact tgtcgaaaaa
     1921 ttgcccggcc cgcgactgaa tgtaggccac ttctttgtcg gtaaaagagt gagtttttcc
     1981 gtcaagacca acgaagtttc tcacatcttt acgacccaaa taagcgtcga gttgcttttt
     2041 caaattctcg gacaatcgcc cactcatgcc ctgaatcgag gcgactcctg acatgtaggg
     2101 cttcgaaaca ggcaacaacg taaagtacgc ccattccaaa ccaccaatcg ctttgagctg
     2161 tgcctttaca tagtctagct gttgcgcggc gatagtttct tccatgttga gaccgtcgag
     2221 caccgcgtgc tgacgatcaa tagcaatgaa atacgcttct gaagaaagcg gatatagtgc
     2281 cctgaacaag cgaactgcgg cgtttcgtcc atacacatgc gggcttaacg cgaccgcatc
     2341 caatggaata gcatctcgcg gatcatgcgg ggccttggcc gccatgtagc gctccaccaa
     2401 cgtggtgggt agcattgcta cgctggaaac cgattcataa gcccagtttg cgatcggaga
     2461 agccgaactg tcgaacgtat cgcagtccaa ataaccaagc atcgcgtggc tgtcagtgca
     2521 aaacaacggg cttttttcag ccatttgctg atcggaatag ccccactgaa tagcagcttt
     2581 gtcataggcc aacgcttcac cgcccgactt tatttgagct gcggcaagaa cgtcgtccat
     2641 gaagtgcatg tattccatta cagagctacc gaatacgtgt tcactatttg gaactgtgtt
     2701 ttttaacaca tattcacgaa atggagcgtc acgttgcgga atagtaacac ttgctccgag
     2761 actgccggcg aaattatggc gcagtccaag tgtatggccc acttcgtgcg ctgttactaa
     2821 gcgtacgtag tcctgcgata cccgaagaag cgtcgcatcg tctgcatttg cttcaagcaa
     2881 aggctcgagg tttgctgctg ctgcttcgtc gagtgagtag ttacacaaac gagcaggtcg
     2941 catgaactca agtgccacgc ccttgtaaag cgggcgcttg gttcgatttt tcggatgagc
     3001 tgtttcgagc agctcttgtc gactgtacgg tgtttcttct tcggcatttt ctagcatgcc
     3061 gtgctgatga tcggtgtggt cacggtgatc atttcgcgcc atggccgccg ggcggtaagt
     3121 gcgcaagaaa cgacgcaaac gcgcctttga tccgaatgca aacgcactcg tcaaatacac
     3181 ttgtgcgttt ttgatttcac ccgtgcgggg gtccatcaac gcgtcagcgt aagcaaaacc
     3241 cgcgctgtcc caaggtaccc actgaattaa attgtagctt gggtcgggag cagtgattcc
     3301 agcaggtgcg acttcggctc gtaaaacttc gcgaccaaaa gccttgttcc aatagaggat
     3361 gccttcttta actgcgtcga cgtattcagc cggcgtattt gcgctgacgt aaaacgtaac
     3421 gggcttagag atatcccatt tggagatctt aatcgaagtg cgaccagatt cttcctcgag
     3481 agttggagga acttcgaagt aaccgactcg gtcgaaagtt ttatcggttt ctttggctac
     3541 gtacttcgga ttttctaagt atggggtgag gtaataacgt acttcgtaag acggagctaa
     3601 gacgaaacct ctattgaatc caatctgaac caattggcga atttccacta agttgttttt
     3661 caaacgcact gaatcgatga aactattgga aactcctaaa gccacgaagt tcgccatggg
     3721 atcgtaacca ccacccgaaa ataatttctc gatatcactc gcgtaccagt tgctggccac
     3781 gaaaacctta ctcatgccct tattg
//
LOCUS       DAOMXO010000170         3797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13209_length_3797_cov_4.532603, whole genome shotgun sequence.
ACCESSION   DAOMXO010000170 DAOMXO010000000
VERSION     DAOMXO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13209_length_3797_cov_4.532603"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..744
                     /locus_tag="PLH57_04675"
     CDS             235..744
                     /locus_tag="PLH57_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRK01937.1"
                     /translation="MIKLNRTTEYSLLSLSYIRAKENAQPASAREISEHFHLPFEILA
                     KTLQRLKEQNIIASTFGTRGGYVLARDLKLLDLASFLKMVEGPLAVVSCCASEETACE
                     FSSGCSIQNPMQALNARIADFLSRISVEELTRPGVSSLNVLSGPTTTNTNRTPEVVQL
                     GSLAYGEEP"
     gene            753..1988
                     /locus_tag="PLH57_04680"
     CDS             753..1988
                     /locus_tag="PLH57_04680"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRK01938.1"
                     /translation="MKNNTIKKFPIYLDYQATTPVDSRVLDAMLPYFTEHFGNAASRS
                     HEYGWRAEEAVEVAREQVAALLGAAAPKEILFTSGATESNNLAILGVAEMYRDKGNHI
                     ITSTVEHKAVLDTCKYLETKGFEVTYLPVDQFGRVNVSELRAAIKDSTILISVMLANN
                     EIGTIQPIREIGKIAKEKGILFHCDAVQGVGKIPFNVEEFGVDLVSISAHKMYGPKGV
                     GALYVRRKNPRVRLAEQIHGGGHERGMRSGTLNVPGIVGLGKAAELSNQLLSEEFERI
                     TALRDRLFKTITTKLSDVYLNGHPAERIPGNLNLSFAYVEGESIIMGLKDLAVSSGSA
                     CTSASLEPSYVLKAIGVGEELAHTSIRFGIGRQTTEAEIDYAANRVIETISKLRDLSP
                     LWEMAQEGIDLKSIQWTAH"
     gene            2006..2398
                     /gene="iscU"
                     /locus_tag="PLH57_04685"
     CDS             2006..2398
                     /gene="iscU"
                     /locus_tag="PLH57_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011714615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HRK01939.1"
                     /translation="MAYSKKVLDHFDNPRNMGSLDKTAQDVGTGVVGAPECGDVMKLQ
                     VKVNDNGIIEDAKFKTFGCGSAIASSSLATEWIIGKSVEDAMKIKNTDIVNELALPPI
                     KIHCSVLAEDAIKAAVRDYLKKKEQKSA"
     gene            2395..2724
                     /locus_tag="PLH57_04690"
     CDS             2395..2724
                     /locus_tag="PLH57_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRK01940.1"
                     /translation="MITVSQNAVKEILRLMESDPSSKESYIRVMVAGGGCSGMSYKLG
                     FESTPVAETDKVFEQDGVKVVVDQKSYLYLAGTELDYSGGLNGTGFTFQNPNAKRTCG
                     CGSSFSV"
     gene            2753..3373
                     /locus_tag="PLH57_04695"
     CDS             2753..3373
                     /locus_tag="PLH57_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01941.1"
                     /translation="MHCWNCKTDLSHQSSLGFFACPKCSAPDFIGTDRSPFAVLGAPR
                     QYDLDLSELEERYLALSKWLHPDRYATLGNTWTKKCGELSALVNESYESLKNSESRLD
                     ALLVGSSDLAKTNSDAIPTRYAERFFEMQELRMEDAAAARALALSLQSEIKQEDNALL
                     EQIDRIARSLNWNKPLEKQSLLTLMETRAKRAYLRSLIANLQKVLL"
     gene            3370..>3797
                     /locus_tag="PLH57_04700"
     CDS             3370..>3797
                     /locus_tag="PLH57_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRK01942.1"
                     /translation="MSSSFKELPVISAGPMTHRPSSQDPIIGIDLGTTNSLVAIVKNN
                     TPIVLPSREGRNLIPSIVHYGADNNIEVGFSAKKRRSTDPKNTIYSAKRLLGRGAADM
                     LDSGIEFPYDLDLSSPQNVQIVIGEKKITAIEVSAEILRE"
BASE COUNT         1083 a          872 c          900 g          942 t
ORIGIN      
        1 aaattactct ggtctagaag gggtgccact aacggtggtt aaataaaaaa gactttaata
       61 ttgaatacat agacctaatt gacaggtgca aacgcattaa gtacgatata aatagtcgta
      121 gtttgacgac ttagccttgt tcgtacgggg caatgtgtcc attttacttg aactccgatg
      181 atttaaatcg tatttaatgg gaacttttaa ccttgagcgt gggagccact aaacgtgata
      241 aaactcaata gaactactga atatagcctc ttgtctttga gctatatacg cgccaaggaa
      301 aacgctcagc cggcttcggc ccgcgaaatt tctgagcatt ttcacctgcc atttgaaata
      361 ctggccaaaa ctctccagcg tttaaaggaa caaaacatca tcgcctccac ttttggaacg
      421 cggggaggtt acgtgttggc gcgtgacctc aagctactcg accttgcttc atttctaaaa
      481 atggtcgaag gccccctcgc cgtggtctcg tgctgtgctt ccgaagaaac cgcgtgtgaa
      541 ttttcttccg gctgttcaat tcaaaacccc atgcaggcat taaatgctcg catcgcggac
      601 ttcttaagtc gtatttcagt ggaagaactg acccgtccgg gtgtaagctc tcttaacgta
      661 ctctcggggc ccacaaccac aaacaccaat cggactccag aggtcgtaca actaggcagt
      721 cttgcatacg gagaagaacc ctaaggggca ttatgaagaa caacacgata aagaaatttc
      781 cgatctactt agactatcaa gccactactc cggtggattc gcgcgtttta gatgcgatgc
      841 ttccttactt tactgaacac tttggtaatg cggccagtcg cagtcatgaa tatgggtggc
      901 gagccgaaga agctgtggaa gtggcgcgcg aacaagttgc agcacttttg ggtgcggcgg
      961 ctcctaaaga aatactcttc actagtggcg ccactgaatc taacaatctc gctatcttgg
     1021 gcgttgccga aatgtaccgc gacaagggca accacatcat tacgtcgaca gtcgaacaca
     1081 aggcagtact agacacctgc aagtatcttg agacaaaggg tttcgaagtg acctacttgc
     1141 cagtagacca gttcggacgc gtaaacgtga gcgagttacg ggccgccata aaagattcca
     1201 caatattgat ctccgtaatg ctcgccaata acgaaattgg aaccattcaa ccgattcgtg
     1261 aaatcggaaa aatcgccaaa gaaaagggaa ttcttttcca ctgcgacgcg gtccaaggtg
     1321 taggcaaaat tccatttaac gtagaagagt ttggtgttga tctggtttcg atcagcgcac
     1381 acaaaatgta cggaccaaaa ggggtcggtg ctctttatgt tcggcgcaaa aatccccgcg
     1441 tacgtttggc ggaacaaatt cacggcggcg gacatgaacg cggcatgcga tccggaaccc
     1501 tcaatgtgcc gggaatcgtg ggactcggca aagcagcgga actatcgaat cagttactca
     1561 gcgaagaatt tgaacgcatc accgctctgc gcgacagact ttttaaaacc attacaacga
     1621 aactttctga cgtttattta aacggtcacc ccgctgaacg cataccgggc aacttaaatt
     1681 tgagtttcgc atatgttgaa ggtgagtcca tcattatggg gctcaaagat ttggcagttt
     1741 cttctggatc agcgtgtact tcagcatcac ttgaaccttc ttatgtgctc aaagcaatcg
     1801 gggtgggcga agaattggca catacttcga ttcgtttcgg cattggacgt caaaccaccg
     1861 aagcagaaat cgactatgca gcgaacagag taatcgaaac aatttctaaa ttgagagatc
     1921 tctcaccgct ttgggagatg gctcaagagg gaatcgatct taaatcgatt cagtggaccg
     1981 cacactaagg aactcagagg caattatggc atacagcaaa aaagtattgg atcacttcga
     2041 caacccgcgc aacatgggaa gtctcgataa aaccgcgcaa gatgttggta ccggcgtagt
     2101 gggcgctcct gaatgcggcg acgttatgaa gttacaggtg aaggtcaacg ataacggaat
     2161 tatagaggat gcaaaattta agacttttgg ctgtggaagc gcgattgcta gttcgagcct
     2221 cgcaaccgaa tggattatcg gtaagagcgt ggaagatgcc atgaagatca aaaataccga
     2281 tattgttaat gaactagctt tgccgcctat caaaattcat tgttcggtct tggcggaaga
     2341 tgcgattaaa gctgctgtga gagactatct caaaaagaag gagcaaaaat cggcatgatt
     2401 actgtttctc aaaatgcggt taaagaaatt cttcggctca tggaaagtga ccccagctcg
     2461 aaagaatctt acattcgcgt aatggtcgca ggtggcggat gctcgggcat gtcttataag
     2521 ctgggctttg aatctacgcc ggtggctgaa accgataaag tattcgaaca agatggcgtt
     2581 aaagtggtgg ttgaccaaaa aagctatctc tatctggcgg gcacggaact agattattcc
     2641 ggcggcctca acggcacagg ctttactttt caaaacccga acgcgaagcg aacctgcggc
     2701 tgcggcagtt ctttctcggt ttaaacccaa agcggcgtga gtactcagcc acatgcattg
     2761 ttggaattgt aaaacagacc tttcgcacca gtcatcttta ggtttttttg cgtgtccaaa
     2821 atgttcagca ccggatttta ttggcactga tcggagtcca ttcgcggtac tcggagctcc
     2881 acggcaatat gatcttgatc tttcggagct agaggagcgc tacttagcgt tatccaaatg
     2941 gttgcacccc gatcgttatg caacgctcgg aaacacttgg accaaaaaat gtggcgagct
     3001 ttcggcttta gtcaatgagt cctacgagtc gttgaaaaat agcgagtctc gcctggatgc
     3061 tttattggtc ggcagtagtg atcttgcaaa aacaaatagt gacgctatcc cgactcgata
     3121 cgcagagcgt ttttttgaaa tgcaggaatt gcgcatggaa gacgccgctg ccgctcgagc
     3181 actagctctt tcgctacaat ccgaaatcaa acaagaagat aacgcgcttt tagaacaaat
     3241 tgaccggatt gctcgctcac ttaattggaa taaaccacta gaaaaacagt cgctattaac
     3301 tcttatggaa actcgggcca aaagagcgta tttacgatcg ctcatcgcca atttgcagaa
     3361 agttttatta tgagtagcag ttttaaagaa cttccggtca tatcagcagg tccgatgacc
     3421 caccgtccgt catcgcaaga ccctattatc ggaattgatc tcggcactac caatagtctg
     3481 gttgctattg tcaaaaataa tacgcctatc gtgctgccgt cgcgcgaggg acgcaacctc
     3541 attccgtcga tcgttcacta tggcgccgac aacaatatag aagtaggctt ttcagcaaaa
     3601 aaacgccgaa gcactgatcc caaaaatacg atctattctg caaagcgact tcttggacga
     3661 ggcgccgcgg atatgctcga ttcgggaatt gaatttcctt acgatctaga cctttcttca
     3721 cctcaaaacg tccaaattgt cattggtgag aagaaaatta cagccatcga agtatcggcg
     3781 gagattttaa gagaaat
//
LOCUS       DAOMXO010000171         3792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13232_length_3792_cov_5.289805, whole genome shotgun sequence.
ACCESSION   DAOMXO010000171 DAOMXO010000000
VERSION     DAOMXO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13232_length_3792_cov_5.289805"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PLH57_04705"
     CDS             complement(<1..883)
                     /locus_tag="PLH57_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRK01943.1"
                     /translation="MEFKLPDIGEGVHEGEIVKWLVKEGDSVKVDQPMVEIMTDKATV
                     EIPTSTAGVIQKLNAKEGDVVKVGQVIAVLGSSGSEVAQTQAPAAKPAASAPKPAAPA
                     AKPGASVPKPAVAAAMTPAPAPQRSSVSPTTSSYTATPGNVLATPATRRLARDLGVDL
                     SRVDPTGPHGRVTKEDVQRFGGASAAGPAMPVSNHGTSLARSSASLPARGRAGGGELE
                     TLVPFRGIRRKISEAMSRSRTLIPEFTYVDEVNVSELVAFRKEVKEIAEKQGVKLTYL
                     PFICKALIRAFREFPTLN"
     gene            complement(864..1808)
                     /gene="lipA"
                     /locus_tag="PLH57_04710"
     CDS             complement(864..1808)
                     /gene="lipA"
                     /locus_tag="PLH57_04710"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008320207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRK01944.1"
                     /translation="MMLRRKPDWLKVKAPGGERFGAIKEQLRSLKLHTVCEEARCPNM
                     GECWSGGTATVMLLGDVCTRGCRFCAITSGKPGGVVDAEEPYKVGGMIALSGLEYVVL
                     TSVNRDDLPDGGAAHFSETVRQIKLRSPHILCETLVPDFQGSEAAIRALLLGGVDVYA
                     HNVETVRRLTTRVRDRRAGYDQSLKVLKAAKRIGLELQSSGLIQNPVFTKTSIQLGHG
                     ENDEEVLETLRELRLHDVDVVTFGQYLQPTQKHLPVVEYVTPEKFETWDRISKELGFA
                     YVASGPLVRSSYKAGEYFISNMIRKTRGEEPIHGIQAT"
     gene            1888..2799
                     /locus_tag="PLH57_04715"
     CDS             1888..2799
                     /locus_tag="PLH57_04715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRK01945.1"
                     /translation="MTTHSPESPAAKAAYYDLFDRSLDAILLVSLENLEILQANDATE
                     NVLLDKPESLTGVSLYQFSHADDLVEFQKMIRIAMRRYHPKRFETRLNVGDPTKIIVA
                     EMAASPLKLKDESEVLQVIVRDVTAQKDAEKRLRELATTDGMTGLVNYRQFCKLLETE
                     HLRSDRYKKQYSIVFCDIDHFKNFNDTNGHPAGDDLLREFAGVLETCVRNTDVAARYG
                     GEEFVVLCTETDLKSALIVGERIRSAVADHAFEHGKKQPMGFVSISVGISGFPQSGKT
                     WQEVLKAADEALYKSKEGGRNRVTIAP"
     gene            2837..3277
                     /locus_tag="PLH57_04720"
     CDS             2837..3277
                     /locus_tag="PLH57_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01946.1"
                     /translation="MNDLIVQNFDRFLARPHMIWATLAYVILLVGLSLRKKNRLLHGR
                     LMGFAIFLDLALVLLLQIERNAIDTAVNLRLTPLQMCHVLSSTIATVLYFPTLYFGFK
                     CLRQIGSPTADRPMLRKKHLRFAVPAFIFRTIGFLFMFSLLPKD"
     gene            complement(3263..3478)
                     /locus_tag="PLH57_04725"
     CDS             complement(3263..3478)
                     /locus_tag="PLH57_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01947.1"
                     /translation="MGHNHVFRLCHVCRNINEAEEEILRCGHCGKGFLPVRYFEKLKQ
                     ELERAKIEAAGTIEIPMNPLPGLLVFW"
     gene            complement(3605..>3792)
                     /locus_tag="PLH57_04730"
     CDS             complement(3605..>3792)
                     /locus_tag="PLH57_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01948.1"
                     /translation="APLPTAAPSFVPTAAHNWGFPTFQYPNTTVQTSPSTWFDTKKIV
                     DSWGWNFNLLNSNSFVP"
BASE COUNT          942 a         1012 c          917 g          921 t
ORIGIN      
        1 cattgagcgt tggaaactcg cgaaatgcgc gaattaatgc tttgcagata aacggcaggt
       61 aagtgagttt gacgccttgt ttttctgcga tttctttgac ttctttgcga aaagcgacca
      121 gttcgctgac attcacctcg tcaacatagg taaattcggg aatcaaggta cgagaacgag
      181 acatggcttc ggagattttt cgacgaatgc cacgaaaagg aacaagtgtt tcaagctccc
      241 ctccgcccgc gcgaccacgc gctggcaaag acgcactcga gcgagcaaga gacgttccat
      301 gattagacac cggcatggct ggaccagcgg ccgaagcccc gccaaagcgt tgaacatctt
      361 cctttgtgac tctaccgtgt gggcccgtag ggtcgactcg cgagagatcg acaccaagat
      421 ctcgcgccaa acgccgtgtt gccggagtgg ccaaaacatt gccgggggtt gcagtgtaac
      481 tggaagtcgt cggtgaaacc gacgatcgct gcggagccgg agctggggtc atagcggcgg
      541 ccactgctgg ttttggtacg gacgctcctg gcttggctgc aggggctgct ggttttggcg
      601 cagaagcggc tggcttggct gcaggcgcct gggtctgcgc aacttctgac ccacttgagc
      661 ccaaaactgc gatgacttga ccaactttta cgacatctcc ttctttggcg tttaattttt
      721 gaattacgcc ggcagtcgaa gtaggaattt cgactgtcgc tttatcggtc atgatctcga
      781 ccattggttg gtcaactttt acggagtccc cctctttaac aagccatttg acgatctcac
      841 cttcgtgaac gccctctccg atatcaggta gcttgaattc catgaatagg ttcctctcct
      901 cgtgttttgc gaatcatgtt ggaaatgaaa tactcccctg ctttatagct agatcgcacc
      961 aatggtccgg aagcgacata tgcaaatcct aattccttag aaatacgatc ccaggtttca
     1021 aatttttctg gtgtcacata ttcaaccacg ggcaggtgct tctgggtagg ctgcaaatac
     1081 tgaccgaaag taaccacatc gacgtcatgc aaacgaagct cgcgcagcgt ttctaagact
     1141 tcctcgtcgt tttctccgtg tcccaattga atcgaagttt tagtgaacac cggattttga
     1201 atcaaacccg acgactgaag ttcgaggcca attcgcttgg cagcctttaa gacttttaaa
     1261 ctttggtcat acccagcacg gcgatcgcgc acccgggttg tgaggcggcg aacggtttct
     1321 acgttgtgcg cataaacgtc gactccgccc aggagcagcg cgcgaatagc ggcctccgag
     1381 ccctgaaagt cgggaactaa agtttcacaa agaatatggg gggagcgtaa cttgatttga
     1441 cgcaccgtct ccgaaaaatg tgccgcgcca ccgtctggca agtcatctcg attcacggac
     1501 gtgagcacta catactctag ccccgacaat gcgatcatac cacccacttt atacggctct
     1561 tccgcgtcga ctaccccacc gggtttgcca ctcgtaatag cgcaaaaccg gcacccgcga
     1621 gtgcagacgt cgccaagcaa catgaccgtt gcggtcccgc cggaccagca ctcacccata
     1681 tttgggcatc gtgcttcttc acacacggtg tgtaacttta aagaccgaag ttgttctttg
     1741 atggccccaa agcgttcccc accgggggct ttgaccttga gccaatcggg ttttctgcga
     1801 agcataatcg aaccctgaat acaccactgc gtcagctaaa actcaagggt tcttcaattc
     1861 cggaactttg cccgttacaa tggaccaatg acgacacact cacccgaaag ccctgctgcc
     1921 aaagccgcct actacgacct attcgaccgc tcgctcgacg cgatattact ggtaagtctt
     1981 gaaaatctcg aaattctgca agccaacgac gccaccgaaa atgtcctgct cgacaagccc
     2041 gaaagcctca ccggcgtgag cctctaccag ttttctcacg ccgacgacct ggtggaattt
     2101 caaaaaatga ttcgaattgc catgcgccgc taccacccca aacgctttga aacgcgtttg
     2161 aatgtaggcg atccgactaa aattattgtc gcggaaatgg cggccagtcc gttgaaacta
     2221 aaagacgaat ctgaggtatt gcaagtcatt gtgcgcgacg taaccgccca gaaagacgcc
     2281 gaaaaacgac tacgagaact agcaacgacc gacggaatga ccggtttggt gaattaccgt
     2341 cagttttgta aactgcttga aaccgaacac cttcggtcgg accgttacaa aaaacaatat
     2401 tcgatcgtat tttgcgatat cgaccacttt aaaaacttta acgacaccaa cggccaccct
     2461 gcgggtgatg acctcttacg cgaatttgcc ggagtgctcg aaacctgcgt gcgaaacact
     2521 gacgttgccg cacgctatgg gggcgaagaa ttcgtggtgc tctgtacgga aactgaccta
     2581 aaatcagccc tcatcgtggg agaacgtatt cgttcagcgg tggcagatca cgccttcgag
     2641 catggcaaaa aacagcccat gggatttgtt tcgataagtg ttggtatctc tggctttcca
     2701 caaagcggaa aaacttggca agaagttcta aaggccgccg acgaagcgct ttataaatct
     2761 aaagaaggtg ggcgcaaccg cgtcacgata gcgccttgat ctagaaattt aatattaact
     2821 gtgaaatgaa ttttaaatga atgacctcat cgtccaaaat ttcgaccgtt ttctagcccg
     2881 cccgcatatg atctgggcca ccctcgctta tgtaatatta cttgtgggcc tctcgctgcg
     2941 aaaaaagaac cgactcttgc acggccgtct catgggattt gctatttttt tggatctcgc
     3001 cttagtgcta ctacttcaaa tcgagcgcaa cgctatcgac acggcggtaa acctgcgcct
     3061 cactccacta caaatgtgcc atgtgttatc ttcgaccata gcgaccgtgc tttactttcc
     3121 aaccctttat ttcggtttta aatgccttcg acagatcggt agcccgaccg ccgatcgccc
     3181 gatgcttcga aaaaaacacc tacgctttgc cgtaccggca tttatttttc gcacaatcgg
     3241 atttttgttt atgttttcgc tgctaccaaa agactagaag tccgggcagc ggattcattg
     3301 gaatttcaat tgttcctgcg gcttcgattt tggcccgttc caactcttgt tttaactttt
     3361 cgaagtaccg cactggaaga aacccttttc cgcaatgacc gcagcgaaga atctcttctt
     3421 cggcttcatt gatattgcga caaacatgac acagcctaaa cacgtggttg tgacccatga
     3481 accacatttc ggtatctgac ttttaaactc aagtgtgtcg tatcgactgg gaattctttg
     3541 aaccgaggca ccccctttta gagaggggga tgagggggaa gggggcgcct cgatcagatc
     3601 gtcctcaagg gacgaagcta tttgaattca agaggttgaa gttccaaccc catgaatcca
     3661 caatcttttt ggtatcaaac caggtactag gcgaggtctg aaccgtggta ttcggatact
     3721 gaaacgtcgg gaacccccag ttatgggccg cggtaggcac aaacgaaggt gccgcggtgg
     3781 ggagaggcgc cg
//
LOCUS       DAOMXO010000172         3791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13238_length_3791_cov_6.365364, whole genome shotgun sequence.
ACCESSION   DAOMXO010000172 DAOMXO010000000
VERSION     DAOMXO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13238_length_3791_cov_6.365364"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..398
                     /gene="rpsJ"
                     /locus_tag="PLH57_04735"
     CDS             93..398
                     /gene="rpsJ"
                     /locus_tag="PLH57_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRK01949.1"
                     /translation="MEHKIRIKLKAFDHKVLDQSVWEIVDTAKRTGAKVRGPIPLPTV
                     INKYCVLRSPHIDKKSREQFEIRTHKRLIDILEPTQQTVDSLMKLDLSAGVDVEIKS"
     gene            698..1336
                     /gene="rplC"
                     /locus_tag="PLH57_04740"
     CDS             698..1336
                     /gene="rplC"
                     /locus_tag="PLH57_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRK01950.1"
                     /translation="MRSGIFGLKSGMTQIFNQAGDMVPVTVIDLTPGVVTQVKSTERN
                     GYNAVQIGMLPKKVQRTTKAERGHFKKSGSPGFYHVQELRVDSVSGITEGAIISPDFV
                     KEGDLVDVTATSKGKGFQGGMKRFNMSGGPKSHGASLSHRSLGSIGNRADPAKCFKNK
                     KMPGQMGQEQVTMQNLRVVSVDRENNLMLVKGSIPGPKNGVVLVRKAVKTAG"
     gene            1342..1962
                     /gene="rplD"
                     /locus_tag="PLH57_04745"
     CDS             1342..1962
                     /gene="rplD"
                     /locus_tag="PLH57_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRK01951.1"
                     /translation="MAKFDVVNLKNEKVGSVDLPEEVFGVKVNQPLVLQVIKAQLAGL
                     RQGTASTKTKGLIRGGGKKPFKQKGTGNARQGSSRSPLNPGGGSNFGPQPRKYNQRTP
                     KEMVRGALRCVLSDKALAQHLVIVDRFDLSSGKTKDLASTLKKSFKIESALLIDAQNQ
                     KLSLASRNLPRVNYVKTEGTNTYDIVKHEWLIISKESAQALAARLA"
     gene            1974..2267
                     /locus_tag="PLH57_04750"
     CDS             1974..2267
                     /locus_tag="PLH57_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRK01952.1"
                     /translation="METSALSIIKRPIISEKSTIQIESARTYAFEVAKASTKQQVKES
                     IEKAFKVNVLRVRTMIVHGKVKRTGRSVNKRSNWKKALVTLKEGQKIEIFQGV"
     gene            2270..3091
                     /gene="rplB"
                     /locus_tag="PLH57_04755"
     CDS             2270..3091
                     /gene="rplB"
                     /locus_tag="PLH57_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRK01953.1"
                     /translation="MAIKSFKPTSAGLRHKNVADFSMLSKKAPEKSLLEIKKRSGGRN
                     AYGRITTRHIGGGHKRKYRIVDFKRDKMSIPAKVAALEYDPNRTAYLALLNYADGEKR
                     YILAPVDLKVGDSVLASDSADIRPGNTLTLGAMPVGTLLHNLELLPNQGGKLVRSAGG
                     VAQLMGREGIYCQIKMPSGEMRKFHQNCRATIGQLSNPEHMNVAIGKAGRARWMGVRP
                     TVRGVAMNPIDHPHGGGEGKTSGGGHPRTPWGQPTKGYKTRHNKRTDAFIIKRRR"
     gene            3104..3469
                     /gene="rpsS"
                     /locus_tag="PLH57_04760"
     CDS             3104..3469
                     /gene="rpsS"
                     /locus_tag="PLH57_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRK01954.1"
                     /translation="MARSIKKGPFIDDHLLKKVEASRAANSKKVIQTWSRRSTIVPDM
                     VGMTFAVHNGRKFIPVYVTENMVGHKLGEFALTRTFHGHTPADKKAAESGGGAAPGAP
                     AAPAAAAAKPAAAAPAAKA"
     gene            3482..>3791
                     /gene="rplV"
                     /locus_tag="PLH57_04765"
     CDS             3482..>3791
                     /gene="rplV"
                     /locus_tag="PLH57_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRK01955.1"
                     /translation="MEVNASLKFIRVTPRKAMLIADLVRGKGINEALGILKFSRRKRV
                     AGYLTKLLKSAVANADQHGRIDVDTLYIKKLEVGKGPTLKRFRPRAMGRAFGIKKKTC
                     H"
BASE COUNT         1126 a          793 c          983 g          889 t
ORIGIN      
        1 caaatgggga atatggaaat gccccggata ggcgaccagt aggtcttgca gggattgcgg
       61 taaagtaact atcgaagaag ggttaataaa aaatggaaca caaaattaga attaaactca
      121 aagcttttga tcataaagtt ctagatcaat cggtttggga aatcgtggac actgctaaga
      181 gaacaggtgc caaagtacgc ggtccgattc cgttgccaac ggtaatcaat aagtactgcg
      241 tattgaggtc tcctcatatc gacaagaagt cgcgcgagca attcgagatt cgcactcata
      301 agcgtctcat cgacattctc gagccgactc aacagacggt cgacagttta atgaaactag
      361 atctttcagc aggtgttgac gtcgaaatta agtcatagta tagaccagct tccttccgtg
      421 agcataagcg tccacggatg catcctgcag atggcaagtt aggtaactga cgagtgaact
      481 gccaggtgat gctgtggcaa ataaggtgta gacctttcgc caaaaacgaa gacgcaatag
      541 atctgcataa tcgcctcgcg actgggtttc cagtcgcaaa gagggcgaca tgttgtgaat
      601 agttttcgag gtaagtgata gaatgagcga tcaagaaata aacaatacaa cgacagcagc
      661 tggagctagc gcttcaagtg atgtggtgct ttctggcatg cggtccggga ttttcggtct
      721 gaagtccgga atgacacaaa tatttaatca agccggcgac atggttcctg taactgtgat
      781 cgacttgacc ccgggtgtag ttacccaggt taagtctacg gaacgaaatg gttataacgc
      841 tgttcagatc ggtatgttgc caaagaaggt tcaacgtacc accaaggctg aacggggcca
      901 tttcaaaaaa tcaggaagcc ctggttttta ccatgtgcaa gagcttcgcg tagattcggt
      961 tagcggtatc accgagggcg cgattatttc ccctgatttc gtaaaagaag gggatcttgt
     1021 agacgttacg gcaacgagta aaggtaaggg ctttcagggt ggcatgaagc gtttcaatat
     1081 gtcgggtggt ccaaaatcac acggcgcttc cctttcacac agaagcctag gatcaatcgg
     1141 aaatcgagcc gatccagcaa aatgtttcaa aaacaaaaaa atgcccggac agatggggca
     1201 agaacaagtc acgatgcaga atctgcgagt ggtgagcgtt gatcgcgaaa acaacctcat
     1261 gcttgtaaag ggttcaattc cgggtcctaa gaacggcgtt gtactggttc gtaaagcggt
     1321 caagacggca gggtgatatt tatggcaaaa tttgatgtag taaatttaaa gaacgaaaaa
     1381 gtgggctcag tggaccttcc tgaagaagtc ttcggggtaa aagttaacca gcctctagtt
     1441 cttcaagtga tcaaagcaca acttgcaggt cttcggcagg gtacggcttc aacaaaaacg
     1501 aaaggtttaa tccgcggtgg cggtaagaag cctttcaaac aaaaaggtac gggaaatgcc
     1561 cgtcagggtt cgagtcgaag cccattgaat ccgggcggtg gtagcaactt tggaccacag
     1621 cctcgaaagt acaatcaacg aactccgaaa gaaatggtgc gtggcgcact acgttgtgtt
     1681 ctttcggata aagcgctggc gcaacatctt gtgatcgtag accgctttga cttgtcctct
     1741 ggcaagacga aggatctcgc gtccacattg aagaagtcat ttaaaattga aagtgctttg
     1801 ttgattgatg cgcagaacca gaagctttca ctcgcttctc gtaatttacc tcgcgtaaat
     1861 tacgtaaaga ctgaaggcac caatacatac gacatcgtta agcatgagtg gctcatcatt
     1921 tcgaaggagt ccgcacaagc acttgctgcg agattggcgt aaggaatatt tatatggaaa
     1981 catcagcact cagtattatt aagcgaccga tcatcagcga aaaaagtacg attcagattg
     2041 agtctgcacg aacgtatgct tttgaggtgg ccaaggcttc cacaaaacaa caagtgaaag
     2101 aatcaattga aaaagccttt aaggtgaatg tgctcagagt tcgaactatg atcgttcatg
     2161 gaaaagtgaa acgaactggt cgctctgtga acaaacgctc taattggaaa aaagcactcg
     2221 tgacccttaa agaaggtcag aagatcgaaa tttttcaagg tgtataaaaa tggcaattaa
     2281 atcattcaaa ccaacatctg ctggccttcg tcacaaaaac gtggcggatt tttcgatgct
     2341 gtcgaaaaaa gctccagaaa aatctctttt ggaaattaaa aagaggtccg gtggtcgaaa
     2401 tgcgtacggt cgcattacga ctcgtcatat cggcggcgga cataagcgca aataccgtat
     2461 cgtggacttt aagcgtgata aaatgtctat acccgcgaag gttgcagcgc ttgagtacga
     2521 tccaaataga acggcatatc ttgcgctttt aaattatgcg gatggtgaaa aaagatacat
     2581 tttggctccg gttgatctca aggttggcga ttcggttcta gcaagcgatt ccgctgacat
     2641 caggccgggc aacactttga cattgggtgc gatgccagtt ggtacgctcc tacacaatct
     2701 cgagttgctt ccaaatcaag ggggcaagtt ggtccgatcg gctggcggtg tagcccagtt
     2761 gatgggtcgc gagggaatat attgtcagat taaaatgccg tcgggcgaga tgagaaaatt
     2821 tcatcaaaac tgtcgcgcaa caattggtca gctttcaaat ccagagcaca tgaacgttgc
     2881 tataggtaaa gctggacgcg ctagatggat gggcgtgcga ccaacggttc gtggcgtcgc
     2941 gatgaacccg atcgaccacc cacacggcgg tggtgaaggt aaaacatcag gtggtgggca
     3001 tccaagaact ccttggggtc aaccgaccaa gggatacaag acgagacata ataaacgcac
     3061 agatgcattt attatcaagc gtcgaagata agtcggagga acagtggcta ggtcaattaa
     3121 aaaaggtcca tttatagatg accatcttct gaagaaggtc gaagcgtctc gcgcagcgaa
     3181 tagcaaaaaa gttattcaga cttggtcgcg acgttcaacg atcgtaccag atatggttgg
     3241 aatgacgttt gctgtgcaca acggtcgcaa atttattcca gtttatgtca ccgagaatat
     3301 ggtcggccat aaactcgggg agtttgcttt gactcgtacg tttcatggtc atactccagc
     3361 tgataagaag gccgcagagt ctggcggcgg agcggcacca ggagcccctg cagcaccagc
     3421 ggcagctgcc gcaaaaccag ctgcggcggc acccgcagct aaggcttaat gaggttgaac
     3481 catggaagtt aatgcaagtt tgaagtttat tagagtaact ccacgaaagg cgatgctcat
     3541 tgcagatctc gttcgtggaa agggaattaa cgaggcactt gggattttga agttctctcg
     3601 acgtaagagg gtagccggat atcttaccaa gcttcttaag tccgcagtgg caaatgcgga
     3661 ccaacatggg cgtatcgatg ttgacaccct ttacataaaa aaattggaag tcggtaaagg
     3721 gcctaccctg aagcgatttc gtccacgagc catgggaaga gcttttggaa ttaagaaaaa
     3781 gacttgtcac a
//
LOCUS       DAOMXO010000173         3766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13365_length_3766_cov_4.830234, whole genome shotgun sequence.
ACCESSION   DAOMXO010000173 DAOMXO010000000
VERSION     DAOMXO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13365_length_3766_cov_4.830234"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLH57_04770"
     CDS             <1..535
                     /locus_tag="PLH57_04770"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRK01956.1"
                     /translation="MMVPFVGRRLNEFMRNYKHMAAFGLLISDISQGRVVKMPFLGTT
                     VLYNLVPEDVEKIRKGIAFLARVFLEGGAKRVYTLLHGFSEINNAKDLERLENARVRA
                     EDIEGAAFHPLGTCRMAKSQKHGVVDTSHRVFGWEGIYVCDGSVIPTSLGVNPQETIM
                     AFATRFAGIVLGKKITD"
     gene            complement(525..866)
                     /locus_tag="PLH57_04775"
     CDS             complement(525..866)
                     /locus_tag="PLH57_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01957.1"
                     /translation="MAKHQYPDDVTWDDRPRKILANARHHRKKVTLVLGGVFSLVGVI
                     FFATTLFHRPSRSAAPDLAFMRPSDGELPSLSTNKVKKFDYCAELNQRMNALGVRVGA
                     ATVAFCQPISR"
     gene            complement(836..1741)
                     /locus_tag="PLH57_04780"
     CDS             complement(836..1741)
                     /locus_tag="PLH57_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01958.1"
                     /translation="MSECKQNNLSRELSRLRALALAGAPVPSMLSGIETWRRTSEAGS
                     KNQYKVKISNQAEMLEHTLKDAAHSGASVESLLKETLPRVRRLERFAVKMDGLERQFA
                     FQAAIAIVLPWFVVFVQPGAIQFNFLYVIGGFFQCVGALLFYWLLVRSSRSPSIEKDY
                     LFQFLSCVWMGVKCGSSLSAAVMHAFDRLGLKPHEKIYHRYWKLWFEREHNQQAVFEW
                     PLWAAESRSVADILSSLSQSGAPAAEVLSNLLSEIEDDRQDTFEQRLQELPTRLALVF
                     CLSFTPAVFCILIGSLWPSISTLMM"
     gene            complement(1781..2443)
                     /locus_tag="PLH57_04785"
     CDS             complement(1781..2443)
                     /locus_tag="PLH57_04785"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRK01959.1"
                     /translation="MSSTRGRIAVLVLIVGVCVARPVLSKVEVYFSPRGGCERRIVQV
                     IKGAKTQIDVAMYRLNNLTILGELEKAKARGVQIRILVDRVRAMQNKPLLIALKEERK
                     FDIRVHTKNREQHNSFAVVDGEIALSGSYDWYTMAELFNEENCVVIHDPEAVAKFQKH
                     FDGHLWKDNSSYKSDDFFAYMEGERKAPRRPRLNQEDLNAPQDRVPASVSKKKDSFES
                     GS"
     gene            complement(2592..>3766)
                     /locus_tag="PLH57_04790"
     CDS             complement(2592..>3766)
                     /locus_tag="PLH57_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01960.1"
                     /translation="DEYGLDRELKIQIPRRFVVRVPALANDREIPFLGEVVASRREGV
                     GLSEKETIFLRSPGQDLQVGSSYSVLSKPEYIRERKSDRLAYIYQTTGEIRVIGIKDG
                     LYIATVNRAYDVIKRGDRVYPLLPLITDIKPVASRVALEALVITSHSNTVSFTGQFRV
                     VHFDRGIEDGVQVGNVFRMYNYDDPITNEKITESDFLMNADALVVHATAQFSTAIVLR
                     SRDIFKRGDFGVTLVDVSDLETEFVDRTKSLDGMEQSDALDRELDELDELDRNSGEGL
                     GEKEEIEVKQLDHWDSSKPDTTEPNVEPADESPAIDPTQLDRQYESPPGEPSLPNEDA
                     AQPNYQDFTPPAIDEPALDGGTPESGEQSPSSSGDTENDAELDSSTLPTVAEPMEP"
BASE COUNT          960 a          977 c          868 g          961 t
ORIGIN      
        1 catgatggtt ccgttcgtgg gccgccgctt aaacgaattc atgcgaaact acaaacacat
       61 ggcggcgttt ggattattga tctctgatat tagtcaggga agagttgtga aaatgccgtt
      121 tttgggtacc accgtgcttt acaatctggt tccggaagat gtcgaaaaaa tccgaaaagg
      181 tatcgccttt cttgcgcgag tattcctcga gggtggtgcc aaacgtgtgt acaccttgct
      241 tcacggcttc agcgagatca acaatgccaa agatctcgag cgattagaaa acgctcgcgt
      301 tcgcgccgaa gatatcgaag gagctgcttt tcacccactc ggcacctgcc gcatggctaa
      361 gagccaaaag catggcgtag tcgataccag ccacagggtg tttggatggg agggcatcta
      421 tgtctgtgat ggctcggtta tccctacctc tcttggtgta aatcctcagg aaaccatcat
      481 ggcattcgcc actcggtttg cgggcatcgt acttggaaag aaaatcaccg actgatcggt
      541 tggcaaaaag caacggtcgc agcgccgact cgcactccca gcgcgttcat gcgttggttt
      601 agttcggcac agtaatcaaa ttttttaacc ttattagttg atagactcgg taattctccg
      661 tcgctcggac gcatgaacgc caaatcaggt gccgcagaac gagaaggcct atgaaataaa
      721 gtagtcgcga aaaaaatgac tccgactaaa ctaaaaacac ctcccaaaac caacgtaact
      781 tttttcctat ggtgcctagc gttcgccaaa atttttcgtg gacgatcatc ccaggttaca
      841 tcatcagggt actgatgctt ggccataaac ttcctatcaa aatacaaaac accgccggag
      901 taaaactcaa acaaaacacc aacgccaatc gagttgggag ttcttgtagt cgttgttcaa
      961 aggtgtcctg gcgatcatct tcgatttccg acagcagatt cgagagcacc tcggcggcag
     1021 gcgctcccga ttgcgatagc gaacttaaaa tatcggcgac actgcggctc tcggccgccc
     1081 acaggggcca ctcaaatact gcttgctgat tgtgctcgcg ctcaaaccat agcttccaat
     1141 agcgatgata gattttctcg tggggcttta gacccaggcg atcaaacgcg tgcatgacag
     1201 cggcgctcaa actcgaacca catttaacac ccatccatac gcaggataaa aactgaaata
     1261 gataatcttt ttcaatcgag ggactgcgac tactccgcac caggagccaa taaaatagta
     1321 gcgcacctac gcattgaaaa aatcctccga ttacgtagag gaaattaaat tgaattgcgc
     1381 caggctgcac gaacaccacg aaccaaggga gcacgattgc gattgccgct tgaaaagcga
     1441 actgcctttc gagaccatcc atcttgacgg caaaacgttc aaggcgccgc actcgaggta
     1501 gggtctcttt gagcagcgat tctactgaag caccgctgtg agcagcgtct ttcagagtgt
     1561 gttccagcat ttctgcctgg ttcgaaatct ttactttata ctggtttttt gaaccggcct
     1621 cgcttgtccg ccgccaggtt tcaattccag agagcattga aggaacggga gctcccgcaa
     1681 gcgctaacgc ccgcagacgc gataactccc tcgataaatt gttctgtttg cattcgctca
     1741 tggcgaccgc catagagcaa cattcacgcc aattgactga tcaactgccg gactcgaaac
     1801 tatctttttt cttagaaacc gaagcaggca cccggtcctg aggcgcgttc aagtcttctt
     1861 ggtttaagcg tggtcgccga ggtgccttgc gctccccttc catgtaggcg aaaaaatcgt
     1921 cgctcttata agacgagtta tccttccata agtgaccatc aaaatgtttt tggaacttcg
     1981 ccacagcttc gggatcgtga ataactacac agttttcttc attgaataat tcggccatgg
     2041 tgtaccaatc gtaggatcca gagagtgcga tttcaccatc tacaactgcg aatgagttgt
     2101 gttgctcgcg attcttggta tgcactcgaa tatcaaattt ccgttcttcc tttaatgcga
     2161 ttaacaacgg cttgttttgc attgcgcgaa cacggtctac caaaattcga atttgcacgc
     2221 cgcgagcctt ggctttttcg agttcgccca agatcgtaag attgttcaaa cggtacatag
     2281 ctacgtcgat ctgagttttt gcacccttga tgacttgaac aatgcgcctt tcacagcccc
     2341 cccgaggaga aaagtatacc tctactttcg acaacacggg acgggctaca caaacgccta
     2401 caattagaac caaaactgca attcgacctc tggtactact cactcgaact cctagacaaa
     2461 ggctaataat gctaatttac ctatcggtaa ttgctgccta gaacttgagt ggttttttgt
     2521 agctaattgt taagggcaca caagacaacg cgtgagaaca ccttgaaaag acaacatttt
     2581 ttgagaccgg tctaaggttc catcggttct gcaacggtcg gtaaggtcga gctgtctaat
     2641 tctgcgtcgt tctcagtgtc tccactagag ctgggcgatt gctccccaga ttcgggggtt
     2701 ccgccatcga gggcgggttc gtcgatcgct ggaggcgtaa agtcttggta gttaggctga
     2761 gccgcatctt cgtttggaag cgacggttcc cccggggggc tttcatattg gcggtccaac
     2821 tgagtcgggt caatcgccgg actttcgtcg gccggttcaa catttggctc ggtcgtatcg
     2881 ggcttactgg agtcccagtg atccagctgt ttcacttcaa tctcttcttt ttcgcccaaa
     2941 ccttcgccac tatttcgatc caactcgtcg agctcatcta gctcccgatc aagtgcatcg
     3001 gactgttcca ttccgtctaa actcttggtg cgatccacga attctgtttc aagatcggac
     3061 acgtccacta acgttacacc gaaatcgcca cgtttaaaga tatcccgaga tcttaaaacg
     3121 atcgcagtag aaaactgggc cgtcgcgtgc accaccagag cgtccgcgtt catcaggaag
     3181 tcactttcag tgattttctc gttcgtgata ggatcgtcgt aattgtacat tctaaatacg
     3241 ttcccaacct gcacgccatc ttcaatgcct cggtcgaagt gcactacccg aaattgtcca
     3301 gtaaacgaga cggtattgct atgactggta atcacgagtg cctcgagcgc tacgcgggaa
     3361 gcaaccggtt taatgtccgt aatcagtggc aacaacggat acacccgatc gccgcgtttg
     3421 atcacgtcat aggcgcgatt cacggtggca atataaagcc cgtctttaat accgattact
     3481 cgaatttcgc cggtagtttg gtagatataa gccagacgat cagattttcg ttctcgaata
     3541 tattctggtt tggagagcac tgaataacta gatcccactt gcagatcttg acccggagac
     3601 cttagaaaaa ttgtctcttt ttcagagagt ccaacccctt cgcgacgaga agcaaccact
     3661 tcacctaaaa aaggaatctc tcgatcgttc gcaagtgccg gaacacgcac cacgaaacgc
     3721 cgtggaattt gaatttttaa ctctcggtct aagccgtatt cgtcgt
//
LOCUS       DAOMXO010000174         3746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13443_length_3746_cov_5.048496, whole genome shotgun sequence.
ACCESSION   DAOMXO010000174 DAOMXO010000000
VERSION     DAOMXO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13443_length_3746_cov_5.048496"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="PLH57_04795"
     CDS             <1..1349
                     /locus_tag="PLH57_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01961.1"
                     /translation="EKLRSQKTGDSAGRTFIGDSSVKGSHSVEIKSIVTDNGKVTKVL
                     YDADLFSPGSDLVEVDLATLAKRLDVTIDELQSVPVETLYLIQRSLDEKVIDSKRLAE
                     VNKLLKKAHKNGDSSVVTAGKRTVKNTARRADDTSEAVEDAAVGAVDTRRNSPADQPN
                     GSGSANENEVLTQLESERNAHAEAKLTTSAQDFIKNELRFQKERDLNVTYAFTDVSGR
                     VQEIFLRDVSFDSNKGLLIEGSYKASRKKFKFDLNKDVETITKYYGFGNSDDVRNLTI
                     PEILNLNELMTNGKLTATDIGRLKNEPSLASDLLHSRFKSQTISERIKSLDAETQNVD
                     TLITSVDSGNSRHVDYIDEDLNTQTARITKASKSDGTLEVQVRNADGKVVAEKVNLDD
                     LSKQFGVSRKELEALPPGDLVAIHRARKALGDDDMKPLVKKYIKDRAKGKVACPSK"
     gene            1449..>3746
                     /locus_tag="PLH57_04800"
     CDS             1449..>3746
                     /locus_tag="PLH57_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01962.1"
                     /translation="MKFEWPKPYFLYLALTQCVLLCASARGSTGTCDELVQVIAKARK
                     IVIPPKGPAPAPVTASVAKTATTTIDPPSNAVEISFKYIAPNGKLFTHRMVGKVTEVK
                     TNATGHVESISVKGTVKAQTADGRWFVFDDQSNVTQVFKLDEITDFRNFYNRANQDAV
                     EDLLKNLGTRFEKAGTINVLENGQTKQINGKVVGATRNADGKITKIRLRYSANENIVE
                     SEFDLAAIESMDFKPKSQWYLDLPRAEQNQPKVPRAPAVAVARVAANGVTAKTANLKD
                     PALELPSNAVELSFKYTAPNGTKDVQKITGRVVGFTRDGSGEIESVTVQGRVQAQAAN
                     GRWFVFDDQTNVTQVFKREEITDFRNFYDRATQDAIDDLLKLTGTQYEKAGFFEILEN
                     GITKRINGKVVGVVRNELGKITKVRVRTLLDPSVRSVDSAKVGTIEIALSDIQKIDFK
                     PRSRWYHTAQSGGGDKSGGIIVEDRTNSPGSEVPNRNSDSVPPNDTEQPAPAAENVVQ
                     IPLREPTRRWPDPPPGFPRGSTKDGTVDGGIAGGQSGDGGGKPKNKPDVENEGLRGDR
                     NDVANEKNTEPAVSEANEAPQPPEKSPNREYARSGREEPFETDRELANEEKPVTKPFD
                     TNRLPLKPTRLPSNAVSNPVAFADTNPHISVDEINSANRANSVLDALPAAPDSIVVRI
                     DDSGEIVLPVLRDLEERPDVADVPLTVVTHEPQAPLVLSPNVNVPKDLPPPLPLRTPK
                     VRNQIPEDLDKRFSEPADSYLRRIFP"
BASE COUNT         1099 a         1009 c          878 g          760 t
ORIGIN      
        1 aagagaaact ccgaagccaa aaaacagggg actctgcagg taggacgttt atcggagata
       61 gcagcgtcaa aggctcccat tcggtcgaaa taaagtccat cgtgactgac aacggaaaag
      121 ttacgaaggt tctttacgac gccgacttgt tttcccctgg atcggacctc gtagaagttg
      181 atcttgccac gctggcaaaa cgactcgatg taacgatcga cgaactccag tctgttccgg
      241 tcgaaacgct ctatctgatt caacgctcac ttgatgaaaa ggttatcgac tcaaaacgcc
      301 tggccgaagt taacaaacta cttaagaaag cacataaaaa cggagactct agcgttgtga
      361 ctgccggaaa acgcactgtt aaaaacactg cacgccgcgc agatgacaca tcagaagcag
      421 tcgaagacgc cgccgtcgga gccgtggata ctcgcagaaa ttcgccggcg gaccaaccga
      481 atggttctgg ttcagcaaac gaaaatgaag ttttgacgca actagaatca gaacgcaatg
      541 cgcacgcaga ggctaaactt acaacatctg cccaagactt catcaagaat gaattaagat
      601 ttcaaaagga acgagatctc aacgtcacct acgcattcac tgatgtcagc ggacgagttc
      661 aggaaatttt tctacgcgac gttagttttg attctaataa aggacttcta atcgaaggct
      721 cttacaaagc tagtcgcaag aaatttaaat tcgacctcaa taaagacgtt gaaactatca
      781 ctaaatacta cggattcgga aatagcgacg atgtccgtaa tctcacgata ccggaaattc
      841 tgaatctcaa cgaactcatg accaacggca agctcacggc caccgacatt ggtcggttga
      901 agaatgagcc atcgttggcg tcggatttgt tgcactctcg cttcaagagc caaacgattt
      961 ctgagcgtat caaatcactc gacgccgaaa ctcaaaacgt cgacacgttg attacaagcg
     1021 ttgattctgg gaatagccgc cacgtggatt acatcgacga agacctcaac acccaaaccg
     1081 cgcgcatcac caaggcttcg aaaagcgatg gaacccttga agttcaggtt cgcaacgccg
     1141 acggtaaagt ggtcgctgaa aaagtcaacc tcgacgacct ttccaagcag tttggcgttt
     1201 ctcgcaaaga gcttgaagcc ctaccgcctg gcgatctggt tgcgattcac cgcgcgcgca
     1261 aagcactggg cgacgacgat atgaaaccgc tcgtgaagaa atacatcaaa gaccgcgcaa
     1321 aaggaaaagt ggcttgccct agcaagtaag tcgttttcca agccccagcc gacgagcacg
     1381 gagctcgtgg taccgcagga cgttcaccct ttaagcatga cgctcccgga aattccggag
     1441 gttgcccttt gaagttcgaa tggccaaaac cgtattttct ctacctcgct ttaacgcagt
     1501 gcgtattatt gtgtgcatcg gcacgaggtt ctaccggaac ttgcgacgaa ctcgttcagg
     1561 tcatcgccaa ggcacgaaaa atagtcattc cgcccaaggg cccggctcca gcgcctgtca
     1621 ccgccagtgt agccaagacc gcaaccacga ccatcgatcc gcccagcaac gcggtagaaa
     1681 tttcgtttaa atacattgcg cctaacggaa aactcttcac ccaccgcatg gtaggcaagg
     1741 tgaccgaagt taaaacgaac gcaaccgggc acgtcgagag catttcggtt aaaggcaccg
     1801 tcaaagcgca aactgccgac ggccgctggt ttgtgttcga tgatcaatcg aacgtgactc
     1861 aggttttcaa actcgacgaa attactgact tccgcaattt ctacaatcgc gccaatcaag
     1921 acgcggttga agatcttctt aaaaacctcg gaactcgttt tgaaaaagcc ggcaccatca
     1981 atgtgctgga aaacggccag acaaaacaaa tcaatggtaa ggtggtagga gccacccgca
     2041 atgccgacgg taaaataacc aaaattcggc tgcgctattc cgccaatgaa aacatcgtcg
     2101 aatcagaatt tgatttagct gccattgagt cgatggactt taaaccaaaa tctcagtggt
     2161 accttgatct tcctagagcc gaacagaatc aacctaaagt ccctcgagct cccgcggtcg
     2221 ccgtggcccg ggtggcagca aatggcgtca ccgccaaaac cgctaaccta aaagaccccg
     2281 cgctggaact accgtccaac gcagttgaac tttcctttaa gtacacagcg ccgaatggca
     2341 caaaagacgt acaaaaaatc accgggcgcg tcgtcggatt tactcgtgat ggttccggtg
     2401 aaatcgaaag tgtaaccgtt cagggccgcg tgcaagccca ggcagccaat ggtcgttggt
     2461 ttgtatttga tgatcaaaca aacgtcaccc aggtgtttaa acgtgaagag atcacggatt
     2521 ttcgcaattt ctatgaccgc gccactcaag acgcgatcga tgatttgctt aaactcacgg
     2581 gcacgcagta cgaaaaagcc ggctttttcg aaatactaga aaacggaatc accaaacgaa
     2641 ttaacggaaa agtagtgggc gtggtgcgca atgaactcgg caaaatcact aaagttcgcg
     2701 tgcgaacgct tttagacccg agcgttcgga gtgtcgatag cgctaaagtt ggtacaattg
     2761 aaatcgcttt atccgacatt caaaaaattg acttcaaacc aaggtctcgc tggtaccata
     2821 ctgcgcaaag cggcggaggc gacaaatctg gcggtatcat tgtagaagat cgaactaatt
     2881 ctccgggatc agaggttccc aatagaaact ccgattcggt gccaccaaac gacacggaac
     2941 aaccggcacc cgcagccgaa aatgtcgtac aaattcctct acgtgaacct acccgtcgct
     3001 ggcccgaccc accgcccggt tttccgcgtg gttctaccaa agatggcacc gttgatggcg
     3061 gaatcgccgg cggccaaagc ggcgatggtg gaggcaaacc aaaaaataaa cccgacgtcg
     3121 aaaacgaagg tctccgtggt gatcgcaacg acgtcgccaa cgaaaaaaac accgaaccag
     3181 cggtgagcga ggccaacgaa gcgccccagc cccctgaaaa gtcacccaat cgcgaatacg
     3241 cccgttccgg tcgtgaagaa ccgttcgaaa ctgaccgaga actggcgaac gaagaaaaac
     3301 ctgtgactaa gccgttcgat acaaaccggc ttcctttaaa gccaacaaga ttaccatcga
     3361 atgctgttag taatccggtt gcatttgccg acacaaatcc gcacatttcg gtagacgaaa
     3421 tcaattcagc caatcgagct aattcagtgc tcgacgcact gccagcagca ccagattcga
     3481 tcgtcgtacg cattgacgac agcggtgaaa tcgtacttcc cgtgcttcgc gaccttgaag
     3541 agcgccccga cgtggccgac gttccactga ccgttgtaac tcacgaacca caagcgccac
     3601 ttgtactgag ccccaatgta aacgtgccga aggacttacc tcctccactc ccgctgcgaa
     3661 cgccaaaagt acgaaatcaa attcctgaag acctcgacaa gcgttttagc gagccggcgg
     3721 attcatacct tagaagaatt tttcca
//
LOCUS       DAOMXO010000175         3704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13660_length_3704_cov_4.984105, whole genome shotgun sequence.
ACCESSION   DAOMXO010000175 DAOMXO010000000
VERSION     DAOMXO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13660_length_3704_cov_4.984105"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1380)
                     /gene="serS"
                     /locus_tag="PLH57_04805"
     CDS             complement(106..1380)
                     /gene="serS"
                     /locus_tag="PLH57_04805"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRK01963.1"
                     /translation="MLDARYFGEKIEDLEKALAKRHADRSLVEQIKTKAESRKKAISE
                     VERLKAYRNQASQEIAAAKKSGQNVDAKLAEMKGVADQIKVLDSQAAQIDEDFKSLAL
                     VMPNIPHASVPTGKTAEDNVKAREWGSPKKFNFQPKDHTDLGSALGILDFDRAAKITG
                     ARFALLKGIGAKLERALMNFMLDIHVNEHGYTETWTPLMVNRQALVGTGQLPKFEQDL
                     FKIEGQDYFLIPTAEVPVTNIFRDEILDGKDLPLKFTAYTPCFRSEAGSYGKDTRGLI
                     RQHQFDKVEIVKFTHPDTSYDEHEKLVRDAEVILQRLELPYRVMTLCGGDLGFGAAKC
                     YDLEVWVPSQNAYREISSCSNFEDFQARRANIRFRETPTDKPRFVHTLNGSGLAIGRT
                     WLAILENYQQEDGSIIVPKALQSYVGTNVLKS"
     gene            complement(1396..>3704)
                     /locus_tag="PLH57_04810"
     CDS             complement(1396..>3704)
                     /locus_tag="PLH57_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK01964.1"
                     /translation="FILFAPAEKKPEKQIPFKVTMPAPKEKSDPQKSSQLYVSALKFY
                     NEDTVEGYKKAAGILLESVANDSDNVKALCLLASAYMHLIDVVDRDENYFNVVTRLIE
                     LERAKNVNLPETVVADVELYLILGNPDAAMSRIVEFTKNRTFGVEMFYYLALSFFHKG
                     MHPDALKYLNNIDPKDYFSPRIHYLYGQIYYHSRQLENAVSSLESAIQKSPKHIKARV
                     LLADTYFKKDSYEKVVEHSSFVIQNKALASNEEEARSYYLRARSYILLNKLVEAHSDL
                     QKAHKLSPDDQDILLEFYTLKARVGEQVGEAASRARMFSQIAVGERALREGRLKDAKV
                     AFLSARNIQGDDPTPLLRLAEVFKRTGEAAEARAAYEKAVKLGSSSRIDFYPKYIHAL
                     VDAYEFEEAQRQIEIFKGLRPPTYAIDKIYGDFYLRQEKLREAHGYYKRALSATNVDS
                     GVYNSYAKIMFKTGSFREAAFYYGLARRFDPFNPDATIGIGKSLAELEGVERGVGYLS
                     SELATNPNKAALFNGIAEIYVRKGDYETAVKFANNALAVDPKYAQSLKTRGDAFAAQE
                     RYKEALEEYQRYADAAPLDPNGNIERYKIFMRRLNLKDAKDEIQKVIKNYPRFPGAYY
                     MLGELFREAQNWQGALQAADREIQNNPLFAPAYVLGGTVFNANREYTRALEYLNKALR
                     VDPSYVPALIQAGFANQMLKTYAAAKSMYERALAQDPGNPKIHKHLGVLLFELGDRAK
                     AAEYLKNYIDLFPDAPDRAEVERKLQGL"
BASE COUNT          884 a          904 c          827 g         1089 t
ORIGIN      
        1 cgtcccgctc gcctcgatta caaggttccg ctttgcggaa cactcgtcaa ccactcaccc
       61 acctctccgt ttactttatg aaatcagtgc cttaaccccg ggcgcttagg atttcaagac
      121 attagttcca acgtacgact ggagggcctt aggcacaatg atcgagccat cttcttgttg
      181 atagttttct aaaatcgcca gccacgtgcg gccgatagct aaaccggaac cgtttaacgt
      241 atgcacaaag cgcggtttat cggtcggtgt ctctcgaaaa cgaatattgg cgcgccgggc
      301 ttgaaagtct tcaaaattac tacacgatga tatttcgcga taagcgttct gactcggaac
      361 ccacacctcg agatcgtagc acttcgccgc gccgaagccg agatctcccc cgcacaacgt
      421 cattacgcga tagggtaatt caagtctttg caaaatgact tccgcgtcgc gcacgagctt
      481 ttcgtgttcg tcgtagctag tatcggggtg agtaaatttt acgatttcta ctttatcgaa
      541 ttgatgttgt cgaataaggc cgcgggtatc ttttccatac gatccggcct cactacgaaa
      601 acaaggggtg tatgcagtaa atttaagtgg caaatctttg ccgtctaata tttcgtctcg
      661 aaagatatta gttactggaa cttccgcggt gggaattaaa aaataatcct gcccttcgat
      721 tttaaaaaga tcttgttcaa actttggcaa ttgaccagtg cccaccaggg cctggcgatt
      781 taccatcagc ggcgtccacg tttcagtgta accgtgttca tttacatgaa tgtcgagcat
      841 aaaattcata agcgctcgtt cgagttttgc tccgattccc tttagtaacg cgaatctcgc
      901 gccagtaatt ttcgccgctc ggtcgaagtc taaaatgccg agtgccgaac ccaagtccgt
      961 atgatctttc ggctgaaaat taaatttctt aggggatccc cactcgcgag cttttacatt
     1021 atcttccgcg gtctttccgg ttggcacgct tgcgtgggga atgttcggca taactaatgc
     1081 caacgactta aaatcttcgt ctatttgtgc agcttgagaa tcaagaacct tgatctggtc
     1141 tgctacaccc ttcatttcgg ccaacttcgc gtcgacattc tgtccggatt tttttgctgc
     1201 agctatttct tgcgatgctt gatttcgata ggccttaagt cgctcgactt ctgaaatagc
     1261 ttttttccga ctttcggctt tggttttaat ttgttctacc aacgagcgat ctgcgtggcg
     1321 cttcgccaag gccttttcta gatcttctat tttttcacca aaatacctgg catctaacat
     1381 ctgagactcc cgatactaca atccctgaag cttccgctcg acctctgcac ggtcgggggc
     1441 gtctggaaat aaatcgatat aattttttaa gtattccgct gcttttgcac ggtcgcccaa
     1501 ctcaaacaac aaaacaccta ggtgtttgtg aattttcgga ttacccggat cttgcgccaa
     1561 agctcgctcg tacattgact tggcggcagc atatgtcttc aacatttgat ttgcaaatcc
     1621 agcctgaatc aacgccggca cgtaactagg gtctacgcgt aaggctttgt tgaggtactc
     1681 aagtgcccgg gtgtattccc gatttgcatt aaacacggtc ccgccgagaa cataggcggg
     1741 ggcaaataat gggttatttt gaatctctcg atcagcggcc tgcagggctc cttgccagtt
     1801 ttgagcctcc cgaaacagtt cgcccaacat atagtaggcg cccgggaatc ggggatagtt
     1861 tttaatcacc ttttgaattt cgtcttttgc gtcttttaaa ttgagacgtc gcatgaatat
     1921 tttgtagcgc tcaatgttgc cattggggtc tagcggcgcc gcatcggcgt atcgttgata
     1981 ttcctcgagt gcttctttgt accgctcctg tgcggcgaac gcatcgcctc ttgttttgag
     2041 tgactgcgca tactttggat caacggccag cgcgttattg gcgaacttaa ctgcggtttc
     2101 gtagtcacct tttcgcacat atatttcggc tattccgttg aacagcgctg ctttgttggg
     2161 attggtagcg agctcgctcg acaaataacc caccccccgt tcaacgcctt ctagctctgc
     2221 cagtgattta ccgataccaa tggtagcgtc cgggttaaat ggatcaaatc gtctggcaag
     2281 accataataa aaagccgcct cccgaaaact gccggtctta aacatgattt tcgcgtaaga
     2341 gttgtaaaca cctgaatcta catttgtggc gcttaaagcg cgcttgtagt aaccgtgcgc
     2401 ttcccgcaat ttttcttggc gcaaataaaa atccccgtaa attttatcga tcgcataagt
     2461 cgggggccgc aggcctttga aaatttcaat ttgacgttgc gcttcctcaa attcatacgc
     2521 gtccaccaag gcgtgaatgt acttggggta gaaatctatg cgactagaag accctaactt
     2581 aacggccttt tcgtaggctg ctcttgcttc ggcagcttca ccggtacgtt taaaaacttc
     2641 ggctagacgc agtaatgggg tagggtcgtc accctgaatg tttctcgcgg aaaggaacgc
     2701 tactttcgcg tctttcagac ggccctctct taaagctcgc tccccaactg caatttgact
     2761 aaacattcgc gcacgacttg cggcttcacc cacttgttcg ccaactcggg ctttgagagt
     2821 gtaaaactcg agtagaatgt cttgatcatc cggcgaaagt ttgtgggctt tttgtaggtc
     2881 ggaatgagcc tcgacgagtt tattcagaag tatgtagctt cgagctctta ggtaataact
     2941 tcgagcttct tcttcgtttg aagctagtgc tttgttttga ataacaaaac tgctgtgttc
     3001 gaccactttt tcataggaat cttttttaaa ataagtgtcg gccaaaagca ctcttgcttt
     3061 aatgtgttta gggctcttct gaatggcgga ttctaacgaa ctgacagcat tttccaactg
     3121 tcgcgagtga taataaattt ggccataaag atagtgaatc ctgggactaa agtaatcttt
     3181 cggatcaata ttgtttaaat acttaagagc gtccggatgc atccctttgt gaaaaaaact
     3241 aagggcgaga taatagaaca tttctacgcc aaacgttcta ttctttgtaa attccacgat
     3301 tcggctcatg gccgcatcgg ggttccctag aataaggtaa agctcaacgt cggccactac
     3361 cgtttcgggt aaatttacat tttttgcccg ctccaattcg attaagcgag tcacgacgtt
     3421 gaagtaattc tcatcgcgat ctactacatc aatgaggtgc atatacgcgc tcgccaaaag
     3481 gcataacgct ttaacattgt ctgaatcgtt ggcaactgat tcaagcagaa tgccagccgc
     3541 tttcttgtaa ccttctaccg tgtcttcgtt gtagaacttc aacgccgaca cgtagagctg
     3601 agaactcttc tggggatcgg atttttcttt gggcgccggc atggtaactt taaatggaat
     3661 ctgtttttcc ggtttttttt cggctggggc aaaaagaatg aaaa
//
LOCUS       DAOMXO010000176         3688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13727_length_3688_cov_5.360308, whole genome shotgun sequence.
ACCESSION   DAOMXO010000176 DAOMXO010000000
VERSION     DAOMXO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13727_length_3688_cov_5.360308"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="PLH57_04815"
     CDS             <1..151
                     /locus_tag="PLH57_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRK01965.1"
                     /translation="ASKLNLEIAEVPIRYLERTYGQTNISRWKHGWLLLKMCFFALGR
                     IKFIR"
     gene            complement(847..1254)
                     /locus_tag="PLH57_04820"
     CDS             complement(847..1254)
                     /locus_tag="PLH57_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HRK01966.1"
                     /translation="MKKEVLKRFKKLFEQQKANVLYNDRVIRDDFHVKDDDRMDDIDQ
                     ASSDVEQSMRMRLRNREVLLIKKIDEALKRIEEGTFGLCHACENEIEFRRLEARPTAT
                     LCISCKEDEERKELHNADGRRHKSLGTAMTRFG"
     gene            complement(1398..1982)
                     /locus_tag="PLH57_04825"
     CDS             complement(1398..1982)
                     /locus_tag="PLH57_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HRK01967.1"
                     /translation="MAFINYNTKEINCKIVYYGCGLSGKTTNLQHIYDQTSTAGKGKL
                     ISLSSENERTLFFDFLPLDVGEVRGMKTRFHLYTIPGQSFYEVSRQFILKGVDGIVFV
                     IDSQRERLEANLESYESLEANLEIQGYDVRKIPVVLQFNKRDLPSAVPIVELSQTFNR
                     LGAPEFEAVAHKGLGVFETLTEITKMVLLDLKGG"
     gene            complement(2149..>3688)
                     /locus_tag="PLH57_04830"
     CDS             complement(2149..>3688)
                     /locus_tag="PLH57_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRK01968.1"
                     /translation="PPLARGSDEEADIIDVVSRFTVQILNTSSSIFAPENQNSHGSGF
                     IAELNEERGLVFTNRHVISGSEYEAQKIEIAFPTGKQGERPEIIQAKIIFVSPVTDFA
                     VLEFPTKSLKRSKKYLRVAKFASVPESNLLTLQGLHVMAFGHPFEGNDISTFGTVSGK
                     WEDSSGSSIQIDAAINPGNSGGPLIELQSQSVIGMNTAIIEGANSVGFAIPMVRLLED
                     FNSWREDSTFARNRIVLADLTAVGEGFLKRLGYDRHIRAKYDRKYFHRNKSAIFVDDI
                     SAKSRLKVGDILLELNDQPIDGKTDQFRRLLARAKNEVKFLVLRAGKIVEIMEPIIEI
                     GLENSKRTTDFVAISGIVFRNYNSLEAWRLTGQEAGVYIDGVIPDTTGDEVDAPLHSA
                     RLRGIIIAHQMHEITTIADLKKLLPKIKKVSLLQMLLHPPVIKFSEDGPSIAIEDKVS
                     GKNLVAAYKEVYPLSVDEVFTDKEFDVEKMKAEFDFDSVKPGKRKWRTWLNCNQIVGA
                     RSKK"
BASE COUNT          870 a          883 c          861 g         1074 t
ORIGIN      
        1 cgcctccaaa ctgaacctcg aaatcgccga ggttccgatt cgctacctcg agcgaaccta
       61 cggtcaaact aacatcagcc gttggaaaca tggttggttg cttttgaaga tgtgtttctt
      121 tgccctgggt cgcattaagt tcattcggta aagaaaccaa actgagccgt tctccccgtg
      181 agagaccccg gtttttgcgt caagctgtac ccgagaccca cctcacgatc gtcctacgca
      241 gttggaaaac aacaatgcta gaaaaggcaa aagtttggct aagactatga gcgcccacgt
      301 cttttgctta agtttttcta gttggatccg atacgtaaaa tacgctttgg tatttttcat
      361 aagggatcac ctcctttctt gcgaaaagga ggtagatcct tctacccagc ccagtttggc
      421 gacaacccct aaagcaaaag ccggaccagc ctcacccgta tttaattgta gactcattta
      481 gtttagtcat ttgatgttat gcgttaatag gggcgccatg aacaaggcgc tagttttatt
      541 cgtttttctg ggttttttca gttctgaaat ggcattttcg atggcgcgca tacgccgttt
      601 tcgtgagcag tcgaaagtta aggttaatac cgggattact gtggggggga ttggctttca
      661 ggggttggtt ctacagaggt cgaccgcaac gcagtgattc gttcggttga cgcacttcaa
      721 agcgtgttgg aatacggaat ttgattccgt aaaaaacagg cgctggtgct gggaggcgtc
      781 cgttccccca agcaccagca tgtcagtcac tccatccgtg gagatcagca cttcaaaatt
      841 cgaatctcag ccgaaccgcg tcatagccgt gcccagactc ttgtgcctgc ggccgtcggc
      901 gttgtgcagt tctttgcgct cttcgtcttc tttgcaagat atgcaaagag tggccgttgg
      961 tcgggcctcc aagcgtcgga attcgatttc gttttcgcat gcgtggcaga gaccgaaagt
     1021 cccttcttcg atgcgtttta atgcttcatc aatttttttg atgagcaata cttcgcgatt
     1081 tctcaaacgc atgcgcatgg actgttccac gtcgcttgaa gcttggtcga tgtcgtccat
     1141 tcggtcgtca tctttcacgt ggaaatcgtc acgaatgacg cgatcgttat acaacacgtt
     1201 cgctttctgc tgttcaaaca acttcttaaa acgcttcaga acttcctttt tcatagtccc
     1261 ccttaccatc tgcttgccgt gcctcgcggc gcggctccat acagcgttgt tgttgtgtcg
     1321 tgttaatacg attactactt tactgtgatc gtcgacactt cggcccctaa tgaccaggtg
     1381 tcgcgatcct cgcttaccta gccacctttc agatccaata ggaccatttt ggttatttcg
     1441 gtcagagttt caaagacgcc taagcctttg tgcgccaccg cttcgaattc gggcgcaccc
     1501 aagcgattga aagtttgtga gagttcaaca ataggaacag cggaaggaag gtctcttttg
     1561 ttgaactgta gaacgacagg aattttgcgg acatcgtatc cttgaatttc gagatttgct
     1621 tcgagactct catagctttc gaggtttgcc tccaacctct ctctttggga atctattaca
     1681 aatactatgc catcaacgcc tttcagaatg aattggcgcg agacttcgta aaaactttgc
     1741 ccgggaatag tgtaaagatg aaaacgcgtt ttcatgccgc gcacttcgcc cacgtccaaa
     1801 ggcaggaaat caaagaacaa tgtgcgttcg ttttcgctgg aaaggctgat cagcttgcct
     1861 tttccagccg tagacgtctg atcgtagata tgctgaagat tcgtggtctt cccactcagg
     1921 ccgcaaccgt aatagacaat cttacagttg atctctttag tattatagtt aatgaacgcc
     1981 attcgttgta attattacaa gttgcaaaat ctcagtcggt gtcgtggtgg ccccgtttgg
     2041 gatcacctag aacaataagg caataccata cctaaatcgg tcgtgcaaaa ttcttgaata
     2101 tctttaataa gttacgacta ttggcgttag gcgctgtgcg tatatccgct acttctttga
     2161 gcgggcacca acgatttgat tgcagttcaa ccaggttcgc cacttgcgtt tgccgggctt
     2221 cacgctgtcg aagtcgaatt ctgctttcat tttttcgacg tcaaattcct tgtcggtgaa
     2281 cacttcgtcg actgaaagcg gatacacttc tttgtaggcg gcaactaagt ttttgcccga
     2341 aactttgtct tcaattgcga tgcttggccc atcttcgctg aattttataa ctggtgggtg
     2401 cagcagcatt tgaaggagcg acactttttt gattttcggt agaagttttt taaggtcagc
     2461 aattgtagta atctcgtgca tctggtgtgc gataatgatt ccccttaggc gggcagaatg
     2521 aagaggggca tcgacttcgt cgccggtagt gtcgggaatt actccgtcaa tgtatactcc
     2581 cgcttcttga ccggtgagtc gccaagcttc gagcgagttg tagtttcgga atacgatccc
     2641 agaaatcgcc acgaaatcag tagttctttt actgttttcc aaaccaattt ctatgatcgg
     2701 ttccatgatt tcgacaatct ttcctgctct taaaacgaga aactttactt cgttttttgc
     2761 tcgcgcaagc aagcgccgaa attgatcggt tttgccatca attggctgat cattgagttc
     2821 cagcaagatg tcgccgactt tgagccggct cttcgccgaa atgtcatcta caaatatggc
     2881 agatttattg cggtgaaagt atttacgatc gtacttagcc ctaatgtgtc gatcataacc
     2941 caggcgcttg agaaagcctt caccgactgc cgtcagatct gccaaaacaa ttcgatttcg
     3001 ggcgaacgtg gaatcctctc gccagctgtt gaaatcttct agcaatcgaa ccattggtat
     3061 tgcgaaacca acgctgttgg cgccctcgat gatagcggtg ttcataccga tcactgattg
     3121 agattgaagc tcaatgagag gaccaccgga attgccaggg ttgatagcgg catcgatttg
     3181 aatggaactg ccactggagt cttcccattt tccgctcacg gtaccgaaag tcgagatgtc
     3241 attgccctcg aatgggtgac caaacgccat tacatgcaaa ccttgaagtg ttaaaagatt
     3301 gctttccgga acactcgcga acttcgccac tcttaggtat ttttttgatc ttttcagaga
     3361 ctttgttgga aactctagaa cggcgaaatc tgtcaccgga cttacgaaaa taattttagc
     3421 ctgtatgatt tctgggcgct ctccctgttt gccggtggga aaggcgattt caattttttg
     3481 tgcctcgtac tcgctcccag aaataacatg tcgattagtg aaaaccaggc cccgttcttc
     3541 attgagttcc gcgataaaac ccgacccgtg ggagttttga ttttctggag cgaatatact
     3601 gctgctggtg ttgagaattt gaactgtgaa acggcttacg acatcaataa tgtcagcttc
     3661 ttcgtcggag cctcgcgcca aggggggg
//
LOCUS       DAOMXO010000177         3684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13746_length_3684_cov_5.357399, whole genome shotgun sequence.
ACCESSION   DAOMXO010000177 DAOMXO010000000
VERSION     DAOMXO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13746_length_3684_cov_5.357399"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1905
                     /gene="uvrA"
                     /locus_tag="PLH57_04835"
     CDS             <1..1905
                     /gene="uvrA"
                     /locus_tag="PLH57_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRK01969.1"
                     /translation="LRARLQDSVETALKLAEGLVAIQLEDGGTEHLFSDKFACVDCGV
                     SYPLPEPRTFSFNSPIGACKKCNGLGYLGNDVEDEDDLLEDDSAETGPAILIRTVCTD
                     CNGTRLKQSSLHFKVGNHNIADLCSLSIKKLASFFDTHSFTKREVLIADRILKEIKDR
                     LSFLLKVGVDYLSLNRSAQTLSGGESQRIRLASQIGSSLNGVIYVLDEPSIGLHQRDN
                     DRLIESLLRLRDMGNTVLVVEHDRDTIEKADHIIDLGPAAGTHGGDIIAQGSVQDIIA
                     NSSSITGRYLNDQAQIPVPKVRRTQTDPTRTIVLSEVAENNLKHVEATIPLGCFICVT
                     GVSGSGKSSLVIDTLYPLLMQALYKSKIPTLRLKGATGLDKIDKVVDIDQSPIGRTPR
                     SNPATYTGTFSLIRDLFSQLPESRARGFKSGRFSFNVKGGRCEKCRGDGLVRVEMHFL
                     SDIYVRCDECEGKRYNNDTLSILYKGKSISNVLDMTIDDAMEFFSKVPLLKQKLKTLS
                     EVGLGYITLGQSATTLSGGEAQRMKLAKELASRSTGNTFYILDEPTTGLHFDDVRKLN
                     EVLHRLVEQGNTVLVIEHNLEVIKTADHVIDLGPEGGELGGEIVAIGTPEQIAQNPKS
                     VTGRYLARYL"
     gene            complement(1963..2256)
                     /locus_tag="PLH57_04840"
     CDS             complement(1963..2256)
                     /locus_tag="PLH57_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRK01970.1"
                     /translation="MRIWLVLVATFLVSCVAFASEGGATDPYASLAAGLAIGIAAFGG
                     SLGQSKAAAAALEGIARNPSASGKIFVPMIIGLALIESLVLYAFVIAFVKIKI"
     gene            complement(2280..2984)
                     /gene="atpB"
                     /locus_tag="PLH57_04845"
     CDS             complement(2280..2984)
                     /gene="atpB"
                     /locus_tag="PLH57_04845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRK01971.1"
                     /translation="MHGEHSFTWISLIPGLEHAPNHVVMTVIVAILAVVMGGIATMQL
                     NAAGGAIIVPESKLTFRNFFDLVAEKLYGFCETTLGEHEAKTYFPVIGTLFIFIFFSN
                     LFGLIPGFLPSTDNINTTLAVGLFVFIFYNIQGFKANGVGYLKHFLGPVWYLAPLMLV
                     IEIVSHVFRPLSLALRLRGNIQGDHVVLGVMSSLVPYLVPVIFMLLGLFVAFVQAFVF
                     CLLTMVYISLSTAHDH"
     gene            complement(2997..3401)
                     /locus_tag="PLH57_04850"
     CDS             complement(2997..3401)
                     /locus_tag="PLH57_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01972.1"
                     /translation="MTQTQAPDHLLLRANAFFGIAGLFFFFQFGDMISVKSFLVGWAI
                     AVVNLELLRRIMKILIAYYNGQKPSKLIYFLVFAKLSFWGVVIAAFSKLDWIHPLPFV
                     IGTLTILVAGFAFGFREFFRDKTATLRVGQQE"
     gene            complement(3379..>3684)
                     /locus_tag="PLH57_04855"
     CDS             complement(3379..>3684)
                     /locus_tag="PLH57_04855"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRK01973.1"
                     /translation="LSDFSRKRSQGFASPNLRLTGIFTHGNGALNFRMYQKFAQAFGM
                     VMQVFFLSLAGFVVGYGLDTVVVVSKPWLSIILAFGGISLGFYRLYKWYINDSNTST"
BASE COUNT         1132 a          833 c          807 g          912 t
ORIGIN      
        1 cttcgagcgc ggctgcaaga ctctgtagaa acggcactta aacttgctga aggtttggtg
       61 gcaatccagt tagaagacgg cggaaccgaa catttatttt cagacaagtt cgcatgcgtg
      121 gactgcggag tgtcttatcc ccttcctgaa ccgagaacat tttcttttaa ttctccgatt
      181 ggtgcctgca aaaaatgcaa tggtctaggc tatctaggca acgacgtcga agatgaagac
      241 gacttgctcg aagacgattc agccgaaacg ggtccggcta ttttaattcg aaccgtgtgt
      301 acggactgca atgggacgcg tctcaaacag agttcactgc acttcaaagt tggtaatcac
      361 aacattgctg atctctgttc gttatctata aaaaaactag cttctttttt tgatactcat
      421 tcgtttacca aaagagaagt tctgattgcc gatcgcatat taaaagaaat taaagatcga
      481 ttgagcttcc tattaaaagt cggcgtcgat tatctttcct tgaatcggtc ggctcaaaca
      541 ttatccggtg gcgaaagtca gcgcattcgt ctcgctagtc agattggttc gtcactaaat
      601 ggcgttattt atgtattaga tgaaccaagt attggtctac accagcgaga taacgatcga
      661 ttaattgaaa gtcttttacg gctacgagat atggggaaca ccgttctggt ggttgaacac
      721 gatcgcgata caatcgaaaa agctgatcat atcatcgacc ttgggccggc ggcaggcacc
      781 catggcggcg acattatcgc ccaaggttcg gttcaggaca ttattgcgaa ctctagttca
      841 ataactgggc gttatttaaa cgatcaggct cagattcctg ttcccaaagt tcgacgtact
      901 caaaccgacc cgacccgaac catcgttcta tcagaagtgg cagagaacaa tttaaaacat
      961 gtagaggcta caattccttt aggctgtttt atttgtgtga cgggtgtaag tggctctgga
     1021 aaatctagtt tagtaataga tacgctttat ccactgctaa tgcaggcttt atataaaagt
     1081 aaaatcccca cgctgcgttt gaagggtgcc actggcttag ataaaattga taaagtagta
     1141 gatatcgacc agtcgccaat cgggcgaacc cctcgaagca atcctgcgac ttataccggt
     1201 acctttagct taattcgaga tctcttttcg caactgccag agtcgcgcgc tcgcggtttt
     1261 aaatcgggca gattttcttt taatgtaaag ggtgggcgct gtgagaaatg tagaggggat
     1321 ggccttgtaa gagttgaaat gcacttctta agcgatattt acgtaaggtg cgatgaatgc
     1381 gaagggaagc ggtacaataa cgacactctc tcaatacttt acaagggcaa gtctatatcc
     1441 aacgtgctcg acatgactat cgacgacgcc atggagtttt tttcgaaggt tccattgctt
     1501 aaacagaaat tgaaaaccct gtcggaggtt gggctcggtt atataacgct cggccaaagt
     1561 gcgacgacat tgtctggtgg tgaagcgcaa cgcatgaagt tagctaagga gctggcatca
     1621 cgttctacgg gtaatacttt ctatattctc gacgaaccga ccacgggctt gcattttgat
     1681 gatgtgcgaa agctcaatga agtacttcat cgtctggtag aacagggcaa tacggtactg
     1741 gtgatcgaac ataacctaga agtaatcaaa acagcggacc acgtcattga tttagggccc
     1801 gaggggggcg aattgggcgg tgagatcgtt gcaattggta cacctgagca gatagcacaa
     1861 aatcccaagt cggttacggg ccggtaccta gctcgttatc tgtaaataaa aaaggccgtc
     1921 tccattcgga aacggccttt ttcgtcgatt attttcagac tatcagatct tgatcttaac
     1981 gaacgcaata acgaatgcgt aaagaacaag tgactcgata agagccaaac cgataatcat
     2041 aggtacaaag attttgcccg aagccgatgg gtttcgtgca atgccttcga gagctgctgc
     2101 tgctgctttg ctttgtccga gagatccacc gaacgcggca ataccaattg caaggcctgc
     2161 agccaacgaa gcgtacggat ccgtagcgcc accttcactt gcaaaagcca cgcagctcac
     2221 taaaaaagta gccaccaata ctaaccaaat tctcatattt ttcactgctc gttctccttt
     2281 caatggtcgt gcgcagttga taacgagatg taaaccattg ttaacagaca aaaaacaaat
     2341 gcctgcacaa aggctacaaa caaacccaag agcatgaaaa tcacgggtac caaatacggc
     2401 accaatgacg acataactcc caataccacg tgatcaccct gaatgtttcc gcgaagacgc
     2461 agcgccaagc tgagagggcg aaacacgtga gaaacaattt caatcaccaa catcagagga
     2521 gccaaatacc aaaccggacc aaggaagtgt tttagatacc caactccgtt agctttgaaa
     2581 ccctgaatat tataaaaaat gaaaacaaat aaaccgaccg ccaacgttgt attgatattg
     2641 tcggtcgaag gtaaaaaccc tggaattaat ccaaaaagat ttgagaaaaa tataaaaata
     2701 aaaagcgtgc ctatcactgg aaaataggtt ttggcttcat gctcacctag ggtcgtttcg
     2761 caaaatccgt aaagcttctc tgcgactaaa tcgaaaaaat tgcgaaaagt gagcttggat
     2821 tccggaacaa tgatggctcc acctgcggca ttaagctgca tagtggcaat cccgcccata
     2881 accacggcta gaatagcaac aattaccgtc atcaccacgt gattcggcgc atgttccagg
     2941 cctggaatca aactaatcca cgtaaatgaa tgttctccgt gcatacgcta aaatccttat
     3001 tcctgctgac ctacacgcaa agtggccgtt ttgtctctaa aaaactctct aaaaccaaat
     3061 gcaaatccgg ctacgaggat cgttagagtc ccaattacaa aggggagggg gtgtatccaa
     3121 tcgagctttg aaaatgcggc gatcaccaca ccccaaaacg aaagttttgc gaatactaaa
     3181 aagtaaatca acttactcgg tttttgaccg ttatagtagg caatcaaaat cttcatgatc
     3241 cgacgaagaa gctcaaggtt aacgacagca atggcccacc ctaccagaaa ggacttaacc
     3301 gaaatcatgt cgccaaattg aaaaaagaaa aatagccccg caataccaaa aaaggcattt
     3361 gctcttaaaa gtaggtgatc aggtgcttgt gtttgagtca ttgatatacc atttataaag
     3421 tcgataaaat ccgaggctaa taccaccaaa agctagtatg atcgaaagcc atggttttga
     3481 tactacgacg acggtgtcta aaccgtaacc aactacaaaa ccggccaggc tcagaaaaaa
     3541 cacctgcata accattccaa atgcctgggc aaacttttga tacattctaa aatttaacgc
     3601 accgttacca tgagtaaaaa tgccggtcaa tcgaaggttg ggactagcga atccttgact
     3661 cctctttcga ctgaagtcgc ttaa
//
LOCUS       DAOMXO010000178         3679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13771_length_3679_cov_5.342991, whole genome shotgun sequence.
ACCESSION   DAOMXO010000178 DAOMXO010000000
VERSION     DAOMXO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13771_length_3679_cov_5.342991"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLH57_04860"
     CDS             <1..945
                     /locus_tag="PLH57_04860"
                     /inference="COORDINATES: protein motif:HMM:NF037113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HRK01974.1"
                     /translation="INAREGDSLEDLRFSDFCANCRKSLAEIETILFVEKEVGRCFCS
                     EECIHDYFQPAVDAMLEEHDRLRSRGDYGDGAGERFSQYRALTLEDPDEVWLNETDSG
                     ERHFTFMSKFRSGEDAFTYVCVCLTIEGVPSFVFLGFATRDEDLVEQYRRGTEIPLES
                     PAAEPEVAGGPEDSAANLADAAANEIGRQLLWEKLFAGEGASSSDLRRSDDIPREQFD
                     QFVQFIEPTMDDPDEIWSFVDPEKQKWLTFISIYKDHEDEETFFSMIVVCRLFENGEM
                     QMVSAFPTIDPDFAQKFRRGVQSVNKAYGVGWTRGNAA"
     gene            complement(952..1488)
                     /locus_tag="PLH57_04865"
     CDS             complement(952..1488)
                     /locus_tag="PLH57_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01975.1"
                     /translation="MQKSGISWLKILLVAVLPLFAGACGPSGPSAATDKNQATRFEAA
                     LSAIAGATANGIDETLWRCPSYDNVTLKGGYAEQDTTGARNYLVCVARNDSSRLLVVP
                     NSSRSICVYPAVKTGTSWSLLENERCAAVYNDVPQIFDFNSPNINDVVIVDQSVNYAF
                     KQCLTGTVSCPAYSQGTP"
     gene            1783..3063
                     /locus_tag="PLH57_04870"
     CDS             1783..3063
                     /locus_tag="PLH57_04870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRK01976.1"
                     /translation="MLYAVAEKLQKTSRKFRKASRARWVRWAGFQTLLLLSLFLLSNS
                     TEFFKSPSGDRVLASVSQLRNWGLLNHVSDSHIHAVDAWKIEKGSREVVVAVVDTGID
                     VNHPDLKANLWKDKETGAHGFDFVSNNANPIDTHGHGTHVAGILGAALNAKAGISGVA
                     HNVSIMAVKYYSNDATGAENLRNSVRALNWAIDHGAKIINYSGGGPEASQEEYRALRR
                     AQEKDILLVVAAGNEKKNSDNPEHYYYPCAYQQLTNVICVAATNIKNELLSSSNWGKN
                     RVDVAAPGEKILSTIPGGKYGYMSGTSQATAFVTGLAALILSKNRSLKPQEVREIIRS
                     TVDRASGLIEKIASGGKVNAYRALAEVNRRSGAKRVIAQKTLTPVKVPAPLAVPTVAS
                     GTKKSTLEKKLEKTVFQPVWVPGFSESEWSKISK"
     gene            3096..>3679
                     /locus_tag="PLH57_04875"
     CDS             3096..>3679
                     /locus_tag="PLH57_04875"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01977.1"
                     /translation="MKPTVRFFLLGALSALFFVLAFPPYGFVPLAFVGLVPFLWAVEA
                     ASTAKKAAWVGAGAMISYVLFGFGWVSYVAVSFGGFPWIVGKIVLVLFALFAELQFVA
                     FAIVARVLVKNVRPTTFGFLSFVVMLSSIYVAFDWWYPKIFPNTLGHSLIAWFSVVQI
                     AEFTGAYGLTFLIIAFNASVYFLLRRRHMRVAAIT"
BASE COUNT          893 a          879 c          970 g          937 t
ORIGIN      
        1 atcaatgcaa gggaagggga ctcgttggaa gacttacgat tttcggattt ttgcgccaat
       61 tgccgtaaaa gcttggccga aatcgagacc attctttttg tggaaaaaga agtcggccgc
      121 tgcttttgca gcgaagaatg cattcacgac tattttcaac ccgctgtcga cgccatgctt
      181 gaagagcatg atcgccttag atcgcgcggc gactatggag acggggctgg cgagcgattc
      241 tcgcagtatc gcgcccttac tttggaagac ccggacgaag tctggctgaa tgaaaccgat
      301 tccggtgagc ggcactttac gttcatgagt aagtttcgaa gcggtgaaga tgcattcacc
      361 tatgtttgcg tctgccttac tatcgaaggg gttcctagtt tcgtatttct gggattcgcg
      421 actcgagacg aagatctagt ggaacagtat cgccgtggta cggaaattcc gctcgagagc
      481 cctgctgccg aacccgaggt ggcgggcggg ccagaggatt cggcggcgaa tctcgccgac
      541 gcggcagcaa acgaaatcgg aagacaactt ttatgggaaa aactctttgc gggtgaagga
      601 gcctcttcta gcgacctacg acgctccgat gatattcccc gagagcaatt tgatcagttc
      661 gtgcagttta tcgagcctac gatggacgat ccagatgaga tttggtcgtt cgttgatcca
      721 gagaaacaaa agtggctgac ctttatttcc atttataaag atcacgaaga cgaagaaacg
      781 tttttttcca tgatcgtagt ttgtcggttg tttgaaaacg gtgaaatgca aatggtctcg
      841 gcgtttccga cgattgatcc cgatttcgct caaaaatttc gccgaggcgt gcaatcagtc
      901 aataaagcct acggcgttgg ttggacccga ggaaacgctg cctaattccc tttaaggcgt
      961 gccttgcgag taagctggac aagaaacagt acccgttaaa cattgcttaa aagcgtagtt
     1021 caccgattga tcgacgatca ccacgtcgtt gatgttcggt gagttgaaat caaaaatttg
     1081 aggtacgtcg ttatagaccg ccgcgcaacg ctcgttttct aatagcgacc agctagtgcc
     1141 agtcttaacg gcagggtaaa cgcaaataga gcggctagag ttcggcacta ccaacaagcg
     1201 agagctgtcg ttgcgagcta cgcaaaccag atagttgcga gcacctgtgg tgtcttgttc
     1261 ggcgtaacca ccttttaaag taacattgtc gtaagaaggg caacgccaaa gggtttcgtc
     1321 gatgccattg gccgtggctc cggcaatggc cgagagggct gcctcaaaac gcgtggcttg
     1381 atttttatct gtggcagcgc tgggtccact cgggccgcac gcccccgcaa aaagtggcaa
     1441 aacagccact aacaatattt ttaaccagct tattccgctt ttctgcatcg tctgctccct
     1501 catctctacg ggctatactc tttcaagccg agtgccagtt ttaaacgggt ctggttcggg
     1561 tgtttcgggc taatcggcaa tggggcctgt gtcgaatgaa cgcggcgccc tgtgcaagtg
     1621 tctaaaatat agacacttct aagggggaaa aaccggggat ttcaacgctc tttgcctatg
     1681 tcgccagacg cgctaatgtg gcactccaat tgctctactg tatcccgtag caagccggcc
     1741 aaatggccgc gcagcagcag tagcagcaga agcgagtggt agatgttgta cgcagtggca
     1801 gaaaaacttc aaaagacctc acggaaattc cgcaaggctt cgcgcgcccg ttgggtacga
     1861 tgggccggtt tccaaacgct tttactgctg agtttgtttt tactctctaa cagcacagaa
     1921 ttctttaagt ccccttcggg cgaccgtgta ctggcttcag tttcgcaact tcgaaattgg
     1981 ggattgctca accatgtgag tgattcacat attcacgcag tcgacgcttg gaaaattgaa
     2041 aagggcagta gagaggttgt ggttgcagtt gtcgacaccg ggatcgacgt taatcacccc
     2101 gatctcaagg caaatctttg gaaggacaaa gagaccggag cccacggctt cgatttcgta
     2161 tccaacaatg ctaacccgat cgatacccat ggtcatggaa cccacgtcgc cggcatcttg
     2221 ggcgcggcgc taaatgccaa ggctggaata tctggtgttg cccataatgt ttcgatcatg
     2281 gccgtaaaat actactccaa cgacgccacc ggtgccgaga acctacgcaa tagcgtgcgc
     2341 gcgcttaatt gggcaatcga tcatggagct aagatcatca actacagtgg tggcggtccc
     2401 gaagcgtcgc aagaagagta ccgcgctttg cgaagagctc aggaaaaaga tattttattg
     2461 gtcgtagcag cagggaatga gaagaaaaat tctgataacc cagagcatta ctactacccg
     2521 tgtgcatacc agcagctcac caacgtaatt tgtgtagccg ctactaatat taagaacgaa
     2581 ctgctctcga gcagcaattg gggcaaaaat cgggtcgacg tagccgcccc cggcgaaaaa
     2641 attcttagca ctattccggg tggaaaatac ggttacatgt cgggcactag ccaggctacc
     2701 gcgtttgtta ccgggttggc cgcgctgatc ttatcgaaga atcgcagtct caagccccaa
     2761 gaggttcggg aaataattag atcgaccgta gaccgggcca gtggactgat cgagaaaata
     2821 gcgtcgggtg gcaaagtgaa cgcttaccga gctttggccg aagtgaatcg ccgttctggc
     2881 gcaaagcgag tgattgcaca aaaaacttta acgccagtaa aagttccagc accgttggca
     2941 gttcctaccg tagcctcagg tacgaagaaa tctacactag aaaaaaagct cgaaaaaacg
     3001 gtatttcagc cggtctgggt tccggggttt tccgaatccg agtggagcaa gatttccaag
     3061 tagactgttc tataaaaacc acttagtttt ttttcatgaa gcccaccgtc agatttttct
     3121 tattaggcgc tctttcggcg ctattttttg tattagcctt tcctccgtat ggtttcgtgc
     3181 ccctcgcgtt cgtgggttta gtaccgtttc tatgggcagt cgaagccgct tctaccgcaa
     3241 aaaaggctgc ctgggtcggc gcgggcgcca tgatttcgta tgtgcttttc ggattcgggt
     3301 gggtcagtta tgtggccgta tcgtttggcg ggttcccgtg gatcgtcggg aaaatagttc
     3361 tcgttttgtt tgcgctgttt gcagaacttc agtttgtagc ttttgcgatc gtggctcgcg
     3421 tacttgtaaa aaacgttaga ccaacgacat ttggtttttt gtcgttcgtc gtcatgttgt
     3481 cttcgatcta tgtggctttt gactggtggt atccaaaaat attcccaaac acattggggc
     3541 actcactcat cgcctggttt tcggttgtac aaatcgctga attcacgggg gcctatggcc
     3601 tgacattttt gatcatcgcg tttaatgcca gcgtttattt tctgttgcgg cgtcgccata
     3661 tgcgggtcgc agctataac
//
LOCUS       DAOMXO010000179         3663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13867_length_3663_cov_4.919069, whole genome shotgun sequence.
ACCESSION   DAOMXO010000179 DAOMXO010000000
VERSION     DAOMXO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13867_length_3663_cov_4.919069"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..504)
                     /locus_tag="PLH57_04880"
     CDS             complement(25..504)
                     /locus_tag="PLH57_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01978.1"
                     /translation="MQGRFSTLMTFVVLALWSTAAPAHQAAEPTRPTRVEDLSLVSER
                     EAAGHVNARVLEYQKLLLEAASIDPESPDSKKRFRDLVVEAHGHLVHAMVWAARVDRL
                     YPEETTIGGRLLDGGFAIVGLGATGLGAAGVMFYGVLAGLNNAFNDVVGHLGRRRAN"
     gene            583..2187
                     /locus_tag="PLH57_04885"
     CDS             583..2187
                     /locus_tag="PLH57_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01979.1"
                     /translation="MRTPGPFRVYVGLVSTGYLDRRIIEKTVRDAMLYVGSSSLNTNA
                     GENLKAALLYSDVEIAHPHWARHSYTHPEIPRAIATTLFGNNDQSVTPYRELQIIAKA
                     APQLSTHRMLARANGNSDHYQARGYYELERAFAGRVAVRTWEELPKARYCLAKRVDQP
                     ARQTLHLDVYNKPIYRILCPKLKTDLMFDGLSGALALLLNHDPTKRIVDSLEISAPDC
                     VVSDGIIAAVGPNRTTGRGHELGVILVANNAVAHDLVAAQIFNLDPNRIEVLQTAIRD
                     GWGPTTVGEIEIGGAGLEGITGLSSKTRFWNFGLQTLAGFETRFQSEHPGFHCQIEKY
                     VESESNITAHSESLVEKALVSWIYKTYDFPKTAMKIAFWPKSPVLTGAASVHEILSSS
                     ARSKIITIGTEASDAFEKAIAFTFWQFPLRGWFRLPYGISIRKVQLRNGHRLLWYSLP
                     CSASELTNTNAFSWLFTIAHRGRMTAHLFRTSYLVDRISCLLRNLFSGTSNTQWPRVL
                     TTRLPHNRWWTVPIRPKTSSAPSPEL"
     gene            complement(2164..3567)
                     /locus_tag="PLH57_04890"
     CDS             complement(2164..3567)
                     /locus_tag="PLH57_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01980.1"
                     /translation="MRVGSWRWSGVLGAFLVILGCVSNASAVSPLKDTPHWRDIHAVE
                     ARDGVRSYVFSYEGVGKEASNPTTIVMQPGYTQTPASVESVARDYLIAGAKVDIGQVR
                     EAGREIVSGKGRYNGLQEIFFNDGVAHLVDVFRRNEGKPIALWGNSMGGLIWLAILTN
                     PHVRKDVVRQAQAVCPPNSPIQWVDDLDGRMIESLQVIGSPSHFRSIQHNNRRLVQFV
                     HKRLDLVVHLAERIPDSVLNFVQRSFDEAYRLDRTGRLRDKMKAAAIRATLVAPVSAI
                     MKYGIADAKYIKKEDLRRFVAKGFSAIPREPGVEFLHAIGDGEILGRRNGHYENPEIM
                     APLEGRPLLQLALFPASIPVLIVSPDDDLFCPTCEQKDLFDGLPDGNKAWLRVPGKGH
                     LYFMEDRLREVNKPTIDFFRDPRGFIERQNKSRAHFSTTHSCSVLLGSGDDEVENTRA
                     ESVGLGARILSQLGRRR"
BASE COUNT          918 a         1043 c          855 g          847 t
ORIGIN      
        1 gttccgctgc atgggcgtcg gtaatcaatt cgccctccgt cgcccaagat gccccacaac
       61 atcgttaaag gcgttattaa gacccgccaa aacaccgtaa aacatgactc ccgctgcacc
      121 cagaccggtg gctccaagtc ctacaattgc aaacccacca tctaacaacc gcccgcctat
      181 ggtcgtttct tcggggtata accgatccac tcgcgccgcc cagaccatgg catgaacaag
      241 atggccgtgg gcctcgacaa ccaaatctcg gaaacgcttt tttgaatctg gtgactcagg
      301 atcgatcgat gccgcttcta agagtagttt ttggtattcc aacacacggg cgtttacgtg
      361 gcctgcggct tcacgctcgc tgacaagact caggtcttca acgcgcgtcg gtcgggttgg
      421 ttccgcggcc tgatgcgccg gcgctgctgt gctccagagc gccagaacaa cgaacgtcat
      481 taaagttgaa aatctcccct gcatgaactc cccctgcccg atctcgtagc actaaaagaa
      541 actaggtgac aagcgagcca gatttttcaa aaatagacac ctatgcgcac accaggacca
      601 tttcgagttt acgtgggtct cgtttccacg ggctatctgg accggcgcat tatcgaaaaa
      661 accgttcggg acgccatgct ttacgtgggg agttcctcgc tcaacacgaa tgccggcgaa
      721 aacctaaaag cggccttact ttattcagac gtggagattg ctcatcctca ctgggcacgc
      781 cacagctaca cccaccctga aattccacga gcaattgcaa ccacgttgtt tgggaacaac
      841 gatcaatcgg ttacgcccta ccgcgaactg caaatcattg cgaaggccgc tccccagctt
      901 tcgacccacc gaatgctggc tcgggcaaac ggcaactcag atcactacca ggcgcggggg
      961 tattacgaac tcgagcgcgc gtttgccgga cgagtcgccg tgcgtacatg ggaagaatta
     1021 ccgaaggccc gatattgttt ggcaaaacgt gtcgatcaac ccgcccgtca gacccttcac
     1081 ctcgacgtat acaacaagcc gatctatcga atcctttgtc ccaaactcaa aactgattta
     1141 atgttcgacg gcctcagcgg agcattggct ttgcttttaa accacgatcc cactaaacga
     1201 atcgtcgact ctttggaaat cagcgcgccc gattgcgtcg tgtctgacgg aattatcgcg
     1261 gcggtcggac cgaatcgcac taccgggcga ggccacgaac tcggagttat tcttgttgca
     1321 aacaacgcag tggcacacga tctagtcgcg gctcaaatat tcaacctcga tccgaatcga
     1381 atcgaagtcc ttcaaactgc cattcgcgat ggctgggggc caacaaccgt cggggaaatt
     1441 gaaattggcg gtgcgggtct agaaggaatt accggacttt ctagtaaaac tcgtttttgg
     1501 aattttggcc ttcaaacatt agccggattt gaaacgcgct ttcaaagcga acaccccggg
     1561 tttcactgcc aaatcgaaaa atacgtcgag agcgagtcaa acatcactgc ccacagcgaa
     1621 tcgctggtcg aaaaagcact cgtaagttgg atttacaaaa cctacgattt tccaaaaact
     1681 gcaatgaaaa ttgcgttttg gccgaagtca ccagtgctca caggagccgc tagtgttcac
     1741 gaaatactct cttcatcagc tcgatcgaaa ataattacga ttggaactga agcttcagat
     1801 gcgttcgaaa aggcgatcgc gttcactttt tggcagtttc cgcttcgagg ctggtttcgc
     1861 ctcccttacg gaatttcaat tcgcaaggtt cagctacgca atggccatcg cctgctttgg
     1921 tatagtcttc cttgttccgc cagcgaactt accaatacca acgccttttc gtggctgttc
     1981 accattgcgc accgtgggcg tatgaccgcc cacttatttc gaacatctta tttggttgat
     2041 cgtatcagct gtttattgcg aaatttattt tcgggaacgt cgaacaccca atggccacga
     2101 gttctaacta ctcgcctacc acacaaccgt tggtggaccg tacccatacg acccaaaaca
     2161 agctcagcgc cgtctcccga gctgtgacag aatccgagct ccgagaccta cactttcggc
     2221 gcgagtattt tccacttcgt cgtcgccact cccgagaagg acactgcagg aatgagtggt
     2281 cgaaaaatgg gcccgcgatt tattctgccg ctcaataaaa cctcgcggat ccctgaaaaa
     2341 gtcgatcgtc ggtttattta cttccctcag tcgatcttcc ataaaataaa ggtggccctt
     2401 gcccggcacc cgcaaccaag ccttgttgcc gtctggtaga ccatcaaata aatctttttg
     2461 ttcgcaggtt ggacagaaca aatcgtcgtc ggggctcacg atcaaaactg gtatcgacgc
     2521 gggaaaaagc gccaactgca aaagcggtcg accttcgagt ggtgccataa tttctgggtt
     2581 ttcgtagtgc ccatttcttc gacctaaaat ttcaccatcc ccgatggcat gtaaaaactc
     2641 tactccgggt tcgcgcggga tcgccgaaaa acctttagcc acgaaacggc ggagatcttc
     2701 ctttttaata tatttggcgt cggcaattcc gtatttcatg atcgccgata ctggggccac
     2761 tagagtggct cgaattgctg cagctttcat cttatcgcga aggcggccgg tgcggtcgag
     2821 gcgataagct tcgtcgaacg atcgctgaac aaagttcagc actgaatcag gaatacgctc
     2881 tgccagatgc actacgagat ccaaccgttt gtgaacaaac tgcaccagtc gtcgattgtt
     2941 gtgctggatc gaacgaaaat ggctgggact gccgattact tgtaaactct cgatcatacg
     3001 gccgtcgaga tcgtcgaccc actgaatcgg cgaattcggc gggcaaacgg cctgagcctg
     3061 acgcactaca tctttgcgca catgggggtt cgtcaaaata gcgagccaaa tcaacccgcc
     3121 catactgttg ccccaaaggg ctatcggttt tccttcgttg cgacgaaaca catcgaccaa
     3181 atgggccaca ccgtcattaa agaaaatttc ctgcaatccg ttgtagcgtc ccttgcccga
     3241 aacgatttct cgccccgcct cgcggacttg gccaatgtcg actttcgctc cagcaatcaa
     3301 ataatcgcgc gcgaccgatt caaccgaggc aggcgtttgc gtgtagccag gttgcataac
     3361 aatcgtggtt ggatttgacg cctccttgcc aacaccttca taggaaaaaa cataggaccg
     3421 aacaccgtcg cgcgcctcga ctgcgtgaat atcgcgccag tgcggcgtat ctttaagcgg
     3481 agataccgcc gatgcgttcg aaacgcaccc gagaataact aaaaaagccc ccaaaacacc
     3541 tgaccaacgc caggaaccca ctctcatgaa gaatctcccc ttgtgtccac tcgaagtatc
     3601 cctactaccg agtctgggta attgagtcaa gaattctaca ttttggggtt tgatctatct
     3661 aag
//
LOCUS       DAOMXO010000180         3661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13881_length_3661_cov_4.922906, whole genome shotgun sequence.
ACCESSION   DAOMXO010000180 DAOMXO010000000
VERSION     DAOMXO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13881_length_3661_cov_4.922906"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..561
                     /locus_tag="PLH57_04895"
                     /pseudo
     CDS             13..561
                     /locus_tag="PLH57_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
     gene            586..1035
                     /locus_tag="PLH57_04900"
     CDS             586..1035
                     /locus_tag="PLH57_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01981.1"
                     /translation="MLLIRSGHKRLLQGSGILALALVLSCASVNSRIDEHRDEFTSYD
                     SDTQLKLQNGDIEPGFTETMVYIAKGHPAEKTQRSSGGKLISVWRYGTSKPQEVRTNV
                     PNSSDSLSGSFGYPEFGPRAAHHPPAHYQNRPRMEIEFENGRVKSWR"
     gene            complement(1032..1829)
                     /locus_tag="PLH57_04905"
     CDS             complement(1032..1829)
                     /locus_tag="PLH57_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01982.1"
                     /translation="MGVAPKQEPTVSFRTRVLVICALFCAIVPLAGCTFFGKQVDENA
                     NLAVGQISGGQSILGSWTFSFAQATATNNPSDAQNITYSFEPNSVLRVEVRNPHYSGF
                     LCTGYGQYRIAGRDVFAYVQAESPQGCGFSSFTHLGAAEVGSQRIVFTSPDGAKAYYF
                     KTRFVPTVSPVGLWDFQGDGGFDWIYFDHNGYFILQTTYESEMYLMVGYFSVAQNGVT
                     LVFFDNLDPASVSTAPIIFDQFMTDGFTLDLIEQTDAGPVTYHGIRL"
     gene            complement(1902..3110)
                     /gene="serB"
                     /locus_tag="PLH57_04910"
     CDS             complement(1902..3110)
                     /gene="serB"
                     /locus_tag="PLH57_04910"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRK01983.1"
                     /translation="MVLDPRKKVILTITGRDAPGITSSLTAILNRDGVLLLDLEQSVT
                     HGLLSLSMVIQFVGEEGDDKAVLKDLLFKAKELGVALDFTVVDGDEMLRSSNHQFAIT
                     LMSDDLQAKHVAAISAVLARRGVNIDSIRKLNERGLFCLEMLTYTSANMDLGALKDEL
                     LKVSTRYPTLDIAVQRESLYRRAKRLVVMDMDSTLIQVEVIDELAKRAGVGDQVAAIT
                     ERAMQGEIGFDESLYERVALLKGLTEADLKHVAEAIPLTPGADILIKTTKRLGYKVAL
                     ISGGFSYFGDYLKEKLGLHYVYTNQLEIIDGKLTGRVVGPIVNAERKAELLEQIARLE
                     EVPLESVIAVGDGANDIPMLKKAGLGIAFNAKLKTRRAAPAAVNQRTLHSILYLLGIT
                     DKDIDEVGRL"
     gene            complement(3157..>3661)
                     /locus_tag="PLH57_04915"
     CDS             complement(3157..>3661)
                     /locus_tag="PLH57_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01984.1"
                     /translation="YIYWDDVSYSQLGGRDKSAQTVCIRLAKRLPDIEHIADFAHELT
                     HAVRLTEEVLHGQAATADEFVAARLGAHGGEADAFATECRVKRDILGKWDELCSPFAS
                     NTDMNVDKVLSALKDGTLAASLTGEPYPVMLTRQYEMLVDRRGGRTPASAIDRTPASA
                     QAEERQK"
BASE COUNT          889 a          956 c          888 g          928 t
ORIGIN      
        1 ggtgcgctcg gaatgcgtct gctcttgttg cttagcatta gctggatcat gaagcttacg
       61 aacccacttt tcacgatatt tcagaaaacc ttttccgggc gcgatctgat cttgatttta
      121 gggggactgt tcctcatcgc aaaagcgacc tacgagattt accacagggt ggaagaacaa
      181 aactcgggtt acggtgtcac cggcgcaaaa gctgcgacct ttggctcgat tgtcctacaa
      241 ataatggtac tcgatattgt attttcgctc gactccgtga tcaccgccgt cggaatggtc
      301 aatcaactct agatcatggt ggtcgcaatg ttcttagcgg tcggggcgat gttgttggca
      361 gcggaccgcg tttctgcctt tattgaacga caccccagcg taaaggtgct ggcgctgtct
      421 tttttggtcc tcatcggcag catgttgttt gcggaaggta tgggcaatca cattccaaag
      481 ggttacatct atttcgccat ggccttttcc ctgggcgtgg agatagtaaa tattaaaact
      541 ggctctagac gtaaagccta gcggctatcc ataggagttt acataatgtt gcttatcaga
      601 agtggccaca agagattgct tcagggctcc ggaatcctcg cgctcgcgct cgttctgtcg
      661 tgtgcctcgg tgaattctag aatcgatgag caccgcgatg agttcacttc ctacgattcc
      721 gacactcaat taaagttaca aaatggagat atcgaacccg ggtttactga aaccatggtt
      781 tatattgcta agggccaccc ggcggagaaa acgcaacgct caagtggcgg aaaacttatc
      841 tcagtgtggc gctacggcac tagtaagcca caggaagtgc gcacgaacgt gcctaattcc
      901 agcgattccc tctctggcag ttttggttac ccagagttcg gaccacgggc cgctcatcac
      961 ccgccggccc actatcaaaa ccgtccgcgc atggaaattg aatttgaaaa cggtcgcgtc
     1021 aagagttggc gctagagcct aatgccgtgg taggtcacgg gccctgcgtc ggtctgctcg
     1081 atcaagtcca acgtaaatcc gtctgtcatg aactgatcga aaataatcgg cgctgtcgac
     1141 acagatgcgg ggtcaaggtt gtcgaaaaac actagcgtca cgccattctg tgcaactgaa
     1201 aagtagccca ccattagata catttcagac tcatatgtgg tctgcagaat gaagtacccg
     1261 ttgtggtcga aataaatcca gtcgaatccg ccgtcgccct ggaaatccca caaccccacg
     1321 ggtgacaccg tcggaacaaa acgggtttta aaataatagg ccttggctcc gtcgggcgaa
     1381 gtaaaaacga ttctctgaga accgacctct gctgctccca aatgggtaaa actcgaaaat
     1441 ccgcagccct gagggctctc ggcttgaacg taggcaaaca catcgcgccc ggcgatgcgg
     1501 tactgaccgt atcccgtgca taaaaaaccg ctgtagtgag ggttgcgaac ctccacccgt
     1561 aagacgctat tgggctcgaa ggaataagta atattttgtg cgtcgctagg attattggta
     1621 gctgtggcct gcgcaaaact aaatgtccaa ctacctaaaa tggactggcc accagagatt
     1681 tgaccaaccg ctagattggc attctcgtca acctgttttc cgaagaaggt gcagccggca
     1741 agtgggacga ttgcgcaaaa cagcgcgcat attacgagaa ctctcgttcg aaaggacacc
     1801 gtaggctcct gtttcggagc aacacccatg tcactcctaa ggaccttatc ggacatttaa
     1861 aattcaaact tggcgggaaa attattccgc ccacatatga gctacagccg tccgacttcg
     1921 tcgatgtctt tgtcggtgat tcccaagagg tacaaaatgg aatgcagggt tcgttgatta
     1981 actgctgccg gagcagctcg gcgggttttt aatttagcgt tgaaagctat tccgagcccc
     2041 gcttttttta gcataggaat gtcatttgct ccgtcgccta ctgcaatcac gctctcgagc
     2101 ggaacttctt ccaggcgggc gatttgttcc aataattcgg cttttcgctc agcatttaca
     2161 attgggccca ctaccctgcc agttaacttg ccgtctatga tttcaagttg attcgtgtag
     2221 acgtaatgca gacctagttt ctctttgaga taatcgccga aataactgaa tccgcccgat
     2281 attagcgcca ccttgtaacc cagtcgttta gttgttttga taagaatatc tgcaccagga
     2341 gtcaggggaa ttgcctcagc cacgtgcttt aaatcggcct cagtgagacc tttcaataga
     2401 gcgactcgtt cgtacaggct ttcgtcaaat ccgatttctc cctgcattgc tcgctctgta
     2461 attgccgcca cttggtcccc aacaccggcc cgtttagcaa gctcgtcaat tacttcaact
     2521 tgaatcagtg tggaatccat gtccatgact accaggcgct tcgccctgcg atacaaactt
     2581 tctcgttgaa ccgcgatgtc tagagtagga taccgagtcg aaaccttcaa aagctcgtct
     2641 ttgagtgcac ccaagtccat gttggcactg gtgtaggtga gcatttctaa acaaaatagt
     2701 cctcgttcgt tcaatttacg aatcgaatca atattcacac cacggcgtgc tagaaccgca
     2761 ctgattgccg ccacgtgctt ggcttgcaaa tcgtcgctca ttagcgtgat ggcaaactgg
     2821 tgattacttg atcgcaacat ctcgtcgcca tccactacgg tgaagtccaa cgccactccc
     2881 aattctttgg ctttgaacaa caaatctttg agtactgcct tgtcatctcc ttcttctccg
     2941 acaaactgaa tcaccatcga aagcgagagg agaccatgag taacggactg ctctaaatct
     3001 agcagcagta caccgtcgcg attaagaata gcggttaaac tggaagtaat acccggtgca
     3061 tcgcggccag taatggtaag aataactttt tttcttgggt ctaaaaccat cgaaacctcg
     3121 tgttttttta gaactacacg gggcgatttg gtttgtctat ttctgacgtt cctcggcttg
     3181 ggccgacgcg ggagtccggt cgatcgctga agctggggtt cggccgccgc gacgatcaac
     3241 cagcatttcg tactgccgag tcaacatcac cggatatggt tctcccgtca gggaggcggc
     3301 cagggttcca tctttaagtg cgcttagcac tttgtcgacg ttcatgtcgg tatttgaggc
     3361 aaaaggcgaa cataactcgt cccacttacc tagaatatct cttttaactc tgcattcggt
     3421 cgcaaacgcg tcggcttcgc cgccatgggc accgagccgc gccgccacga attcgtccgc
     3481 ggtcgctgct tggccgtgca agacctcttc agttaggcgc accgcgtgag tcaactcatg
     3541 agcaaaatca gcgatgtgct cgatatctgg cagccgtttt gccagacgaa tgcacacggt
     3601 ctgagcagat ttgtcgcggc cgccgagctg cgagtacgac acatcgtccc aataaatata
     3661 a
//
LOCUS       DAOMXO010000181         3645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_13972_length_3645_cov_4.064067, whole genome shotgun sequence.
ACCESSION   DAOMXO010000181 DAOMXO010000000
VERSION     DAOMXO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13972_length_3645_cov_4.064067"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /gene="trpS"
                     /locus_tag="PLH57_04920"
     CDS             <1..1316
                     /gene="trpS"
                     /locus_tag="PLH57_04920"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRK01985.1"
                     /translation="MLGNYLGAIRNWVDLQKKYDSLFCVVDLHAITIKQSPTELQDRT
                     LSVAATYLACGVDPSLATVFVQSQVAAHAELSWILTCFSNMGELSRMTQFKDKSAKQK
                     SIGTGLFTYPVLMAADILLYQTQLVPVGQDQKQHLELARDLAIRMNTQVGDPSDPLFV
                     VPEPFIAPVGAKIMSLADPKAKMSKSDEDANATIFLTDSEDVIIKKLKKAVTDSGTDI
                     TYSEDKPGIKNLIDIQVAITGKNSDQIVSEFAGKQYGHLKLATAEIVAACIKPIREKT
                     ETFMKDKGALLGILNKGADKANERAQATLQKVKKAVGFVLSLALISLISGGCASVPAT
                     PEAQLGPMPTTGPVGSTFSKHGEKFVNCGRDAVTIQTGSTQRIDFRFTILPSGVVAKP
                     RIDGMSSPDPDLHGCLLRALAKVQFPKPKDKKPKEIGYTITIRPE"
     gene            complement(1297..1944)
                     /locus_tag="PLH57_04925"
     CDS             complement(1297..1944)
                     /locus_tag="PLH57_04925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK01986.1"
                     /translation="MSIRIRKALTHDDEQIMRRICCETADSGKPINQERWDFWGRWWV
                     EPYFRLERNHCWVAEDELGKVQGYLTGTPNSIVFEIKKLLLSTPRFFWGRDVWKNSRT
                     QEDRRRFLKRALGVERSPFRLFPSEIQKELLLKYPAHLHVNVDAALRGRGVGRELYRA
                     FEQQLSKERVSGVHLLCGAAPVGYYLKLGFQIFSKLEIKPGREVYAMVKAIPAEL"
     gene            complement(1925..2566)
                     /locus_tag="PLH57_04930"
     CDS             complement(1925..2566)
                     /locus_tag="PLH57_04930"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRK01987.1"
                     /translation="MRRCGTCFAIALSIGFSSNAISANISSQKKNLGKYRNTYYYVAE
                     EAAFPSDPRDTPILDKNGDTIAMVHAKYKKALDIEGSGKLTDGRVVNYATRVDGQVRY
                     RVTKAPYGNGVGICKLKPFESIAVDREKIPLGSIVRIKETVGMPLPNGKLHNGLWRAI
                     DVGGAIKKDRVDIFIGFRRHAKYLSDYGVTHLKPLNITLIKKPKKKSCVDQDP"
     gene            complement(2623..3030)
                     /locus_tag="PLH57_04935"
     CDS             complement(2623..3030)
                     /locus_tag="PLH57_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRK01988.1"
                     /translation="MTEQGDGAPKKPHLGPEGRRFPRVSTYKHARIESEGDLAVSISG
                     VVFEVGAQGCGVRSEGELEPGSIVKISLYDQTRLDPVIFRAKIVWRGAIEGTHMKSYG
                     IQFLTVESGRESAAHDEVKTILGGVGSKVQIED"
     gene            complement(3115..>3645)
                     /locus_tag="PLH57_04940"
     CDS             complement(3115..>3645)
                     /locus_tag="PLH57_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRK01989.1"
                     /translation="MGVLIMFAGLWSTTSEFRLRFQGLNSILGERLELWKANWAMVRE
                     HPWFGVGWHHNFELSYAYYDWMKVKHGFSSHAHNNLLDQWATTGLFGVLSFIVLNFLL
                     FKMSLKVYRAKISSRGDDLLPRALGLGFFAAWICLHINGVTQANFWDAKVLHQIGWVS
                     AVTLEIVRRAESRVRV"
BASE COUNT          998 a          935 c          771 g          941 t
ORIGIN      
        1 tgatgcttgg aaactacctc ggtgcaattc gaaactgggt agatcttcaa aaaaaatacg
       61 acagtttgtt ctgtgtggtc gacttacacg ccatcacgat caagcagtcg ccgactgagt
      121 tacaagatcg caccttgtcc gtcgctgcca cttatttggc ttgcggagtt gatccttcgc
      181 tcgcaaccgt attcgtgcaa tcgcaagttg ctgcccacgc tgaattgtct tggattctta
      241 cctgttttag taacatgggc gaactttcga gaatgaccca atttaaagat aaaagcgcta
      301 agcaaaaaag cattggtacc ggactcttta cctacccggt tttaatggct gctgatattc
      361 tgctttatca aactcaattg gtgccagtgg gccaagatca aaaacaacac ctcgaactcg
      421 cgcgtgatct tgcaattcga atgaacaccc aagtcgggga cccaagtgat ccactttttg
      481 tggtgccaga acctttcatt gcgcccgtag gtgctaaaat catgagtctg gccgacccca
      541 aagctaaaat gtcgaaatcc gacgaagacg ccaacgcaac gatattttta actgacagtg
      601 aagacgtcat tattaaaaaa ttaaaaaaag cagtgaccga ctctggaacc gatatcacct
      661 attccgaaga caaaccgggg attaaaaact taatcgatat tcaagttgcc attactggaa
      721 agaactcaga tcagatagtt tccgaattcg caggaaaaca atacgggcat ctcaaactcg
      781 ccaccgctga aattgtggct gcgtgtatta aaccgatacg cgaaaaaacg gagaccttca
      841 tgaaagacaa aggagcactg ctcggaatcc taaataaggg cgctgataaa gccaatgaac
      901 gggcgcaagc gactctgcaa aaagttaaaa aggctgtggg ttttgttctc agtttggcgc
      961 ttatttcact tattagtggc ggctgcgctt cggttcccgc caccccagag gcccagcttg
     1021 gcccaatgcc gaccactggg cctgtaggta gcactttttc gaaacatggc gaaaagtttg
     1081 taaactgtgg tcgtgatgcc gtaactattc aaacgggcag cactcaaaga attgattttc
     1141 gtttcacgat attgccaagc ggagtggttg cgaaaccccg tattgacggt atgtcgagtc
     1201 ccgatcccga cctccatgga tgtctgctac gcgcacttgc taaagtgcag tttccgaaac
     1261 ctaaagacaa aaaacctaaa gaaattggtt acacaatcac aattcggccg gaatagcttt
     1321 aaccattgca tagacctcgc ggcccggttt gatttctaat tttgaaaata tctgaaatcc
     1381 taacttgaga taatatccta cgggtgcagc gccacatagc agatgaacac ccgaaaccct
     1441 ttctttcgac aactgctgtt cgaacgctcg gtacaattca cgaccgacac ctctgccacg
     1501 caaggccgcg tctacgttaa cgtgcaaatg tgctggatat ttaagcaaca attccttttg
     1561 gatttcacta ggaaaaagac gaaacggact gcgctcaaca cctagcgcgc gtttcaaaaa
     1621 gcgccggcga tcctcctgcg ttcttgaatt tttccagacg tcacgccccc aaaaaaaccg
     1681 tggcgtcgag aggagtagtt tttttatttc gaaaactatc gagttgggag ttccggtcaa
     1741 atacccttga acctttccca attcatcttc cgcgacccaa caatggtttc gctctagccg
     1801 aaaatagggt tccacccacc aacgacccca aaaatcccaa cgctcttggt taattggttt
     1861 cccactatcg gccgtttcgc aacaaatgcg ccgcatgatt tgttcatcat catgggttag
     1921 tgctttacgg atcctgatcg acacagctct tcttttttgg tttttttatc agcgtaatat
     1981 ttagaggttt caaatgagtc accccatagt cacttaaata ttttgcgtga cggcgaaatc
     2041 caataaaaat atcaactcga tcctttttaa ttgcgccacc cacgtcgatc gctcgccaca
     2101 gaccgttgtg caatttaccg ttcggaagcg gcatacctac ggtttctttt attcgtacga
     2161 tcgaccccaa gggtattttt tctcgatcca ctgcgatcga ctcaaatggc ttgagtttgc
     2221 aaattcccac tccattacca tagggcgctt tggtaacacg atagcgtact tgtccgtcta
     2281 ctcgtgtcgc gtaattgacc actctaccat ctgttagttt cccagatccc tcaatatcca
     2341 gcgccttttt gtatttcgcg tgtaccatcg cgatggtatc tccattttta tctagtatgg
     2401 gagtatcgcg cggatcgcta ggaaacgccg cttcttctgc gacgtaatag taagtattgc
     2461 ggtatttacc caaattcttt ttctgagaag aaatgttcgc tgaaatcgca ttcgaagaaa
     2521 aaccaattga tagtgcaatt gcgaaacagg tcccacaacg tctcatccgt gagctcgtcg
     2581 agttttccat attgaactat tgtgtcacga gctggtttgg cgtcaatcct cgatttgcac
     2641 tttgcttccg acgccaccta aaatggtttt cacttcgtcg tgcgccgcac tctcgcggcc
     2701 agattccaca gttaaaaact gaatgccgta ggacttcatg tgagtccctt cgatggcacc
     2761 gcgccaaaca atcttggctc gaaaaataac tggatcaaga cgcgtttgat cgtagagaga
     2821 aattttaacg atagaccccg gctcgagttc tccctcggaa cgaacaccgc agccctgcgc
     2881 tcctacttcg aacaccacac ctgatatcga aacagccaga tcgccttcag actcaatgcg
     2941 tgcgtgttta taggtcgaaa cgcgtggaaa cctacgtcct tcggggccta aatgcggttt
     3001 ttttggtgcg ccgtcacctt gctcggtcat ttgggaacgc tcctcgccta acctatattg
     3061 taactcgcct gaattttttc aggtcaaaaa taaaaataca ataaaatcaa acacttatac
     3121 tctaacccta gactccgcac gacgcactat ttcgagagtc accgcgctaa cccacccgat
     3181 ttggtgaagc actttggcgt cccagaaatt ggcctgcgtg accccattta tgtgcaagca
     3241 gatccatgcc gcaaaaaaac caagtccgag cgcgcgtggc aaaagatcat ctccccgcga
     3301 tgagatttta gctcgataaa cttttaaact catcttaaag agcagaaaat ttagaacaat
     3361 aaaagacagc acaccaaaaa ggccggtcgt agcccactga tcgagcaagt tattatgggc
     3421 atgtgacgag aacccgtgtt ttaccttcat ccagtcgtaa taggcatagg agagctcgaa
     3481 gttatggtgc caaccaaccc caaaccacgg gtgttcgcga accatggccc agttagcttt
     3541 ccagagttca agtcgctcac cgagaatgga atttaatccc tggaatcgca ggcgaaattc
     3601 ggaggtagtt gaccaaagcc cggcaaacat tattaatacg cccat
//
LOCUS       DAOMXO010000182         3632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14043_length_3632_cov_4.318423, whole genome shotgun sequence.
ACCESSION   DAOMXO010000182 DAOMXO010000000
VERSION     DAOMXO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14043_length_3632_cov_4.318423"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..2330
                     /locus_tag="PLH57_04945"
     CDS             69..2330
                     /locus_tag="PLH57_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRK01990.1"
                     /translation="MTRRNLDFHSYHLISLLVGWASFQFQAVPLAHLLHAVGFHSLPF
                     LLGLQVLISSLILITVASLGKKFSASSANLALTSALALAFVLGVKLLGTSFNLSSSTW
                     LGVHFVAGQIIVAFLRLGLELYCAERMPVFSGSPSANRATIYFELGTLVAGAMLLAPK
                     QIADLGSFGFVTLPLFLLCILPKSATTQSPQLPLPGRSEKNWIPENGLLRFLAYLLFS
                     IAFAKSLQWLIMAYGITHAGKSDKTLLRYFTGLAFTQALSTIASVTLFSKIFEERQHW
                     RSGYLFYIGIQFLLALGCLAYPINAALILSEIARRAVEHSSLLKSQQLLIQSIPRKIR
                     ANLRHKAEIVGANAGTLLAAGFFYLFTVKTRVVWPSLVLILLICVAAYFSINRLKHLI
                     LVVLRHNLRIGTKDEIIHACAALAGPDFRDGAPWVTRLLEHSQEPDIQIAGVQCLSSM
                     GTPNIDVFKHLSSHPREDVQIHAIRALAELNDYDTKIFLVRRLREEIRTTRDPREYTI
                     NVLSESVGVLSVPYLLEILDGHFNDRIRANAVEVLGRQATNSKDSELQNFLSRYLDPQ
                     TNRRVRANAIVALHANPRYRDRCEREIEALSESKDRFDRGAAAFVIGATSDALISTAF
                     RFGRQTHIYRERIWGWSAAHGHKQMSMLIALLRLGDLRALPHFVNLLIAVPGESPGGL
                     REEAVSNFSTLRESRRLRVLEYLCANHPSEVREVTVLLRTSGRPFKTDLHYLEEFLSE
                     HDGAIKLSNIKAA"
     gene            complement(2338..3162)
                     /locus_tag="PLH57_04950"
     CDS             complement(2338..3162)
                     /locus_tag="PLH57_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01991.1"
                     /translation="MFLLLFWCVLPQAHADESMMAAPVDREDRMVEVNETSTDAQRNA
                     KSGLGETGNADAVRPDSQIPRKRMVRRVFIRRRFYTTPSIGISSIQYKETGVSNYSAI
                     ALTGKISTRYPIRARWDVGGSMYFTLMPLTQNPSTNSARFLGVNGRIGYAPPGIKRPW
                     RLSLLTGFYYTRMFVSNNAFGFKDMVGPQLFPVLHRSFKNGDSGMIYGKYSPVTNNLS
                     VLSLSNRELATGITYAYLMKNKRALAISLDIALLAIDIDRVKTATSSITLGVGINL"
     gene            complement(3224..>3632)
                     /locus_tag="PLH57_04955"
     CDS             complement(3224..>3632)
                     /locus_tag="PLH57_04955"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRK01992.1"
                     /translation="MDFVNASHRPPYIYRQEKIKPLMDARCNPLGHDLNIQVEPATVA
                     LEPGDVILWYTDGVLECTNPEGKTISRNSLVKMVKSLHERHASNAPAICEDFMADTMA
                     FFGPNAANLADDVTIVFGAVPPNVQFQKKSFAA"
BASE COUNT          911 a          912 c          823 g          986 t
ORIGIN      
        1 ctaagaaatt taggcagaat tcacccacgc aattgtttaa aaactaaaaa aataccgata
       61 agtcgtagat gacccggcgt aatttggact ttcattccta tcacttgatt tcactgctcg
      121 tgggttgggc tagctttcaa tttcaagcgg ttccgctagc tcatttatta cacgccgtcg
      181 gctttcattc gttgcctttt ttactgggcc tgcaagtatt gattagctcg ctaattttga
      241 tcaccgttgc gtctctagga aaaaaattca gcgcttcctc ggcaaatttg gccttaactt
      301 cggcgctggc attggcgttc gttttgggag ttaaacttct cggcactagt ttcaacctat
      361 cttcatccac ttggctcggt gtgcacttcg tcgctggtca gatcatcgtg gcctttttga
      421 gattgggcct tgaactctat tgcgcggaac gcatgccagt tttttctgga agtccttccg
      481 caaaccgcgc aaccatttat ttcgaactcg gaactctggt tgcaggcgcc atgttgctgg
      541 cgccaaaaca aattgctgat cttgggtcgt tcgggttcgt cactctgccc ttgttcctac
      601 tttgtatcct gcccaaatca gcaactaccc aaagcccaca gctaccatta ccgggtagat
      661 ctgaaaaaaa ctggattcca gaaaatggcc tacttcgatt tttggcctat ctgttgtttt
      721 ctattgcgtt tgccaaatct ttgcagtggc tcatcatggc ctacggcatt actcacgccg
      781 gtaaatctga taagacacta ttacggtatt ttaccgggct cgcattcacc caggcgctct
      841 caacaatcgc ttcggttacg cttttttcga aaatttttga agaacgacaa cactggcgtt
      901 caggttatct cttttatatc ggcattcaat ttcttttagc tcttgggtgc ctagcttatc
      961 ccatcaatgc ggctttaata ttaagcgaaa tcgctcgacg tgcagtagaa cactcttcgt
     1021 tacttaagtc acagcaactt ttaattcaga gcattcctcg gaagatacga gcaaatttgc
     1081 gacacaaagc tgaaatcgtc ggagcaaacg ccggaactct actggcggcg ggttttttct
     1141 atctgtttac tgtgaaaaca cgagtggttt ggccttcgct tgttttgatt ctccttattt
     1201 gcgtagcggc ctatttttcg atcaatcgac taaaacatct tatactcgtc gtacttcgac
     1261 acaatcttcg gatcggaacc aaagacgaaa tcattcacgc ctgtgcagca ctggcaggtc
     1321 ccgactttcg tgatggtgcc ccatgggtga ctcgcctctt agagcattca caggaacccg
     1381 atattcaaat tgccggtgtt cagtgtttat cgtcgatggg taccccgaac atcgacgtat
     1441 ttaaacactt gtcctctcac ccccgggaag acgtgcagat ccatgcgata cgcgccctag
     1501 cggagttaaa cgactacgac acaaaaatct ttcttgtccg tcggttacgc gaagagatca
     1561 gaaccaccag agatcctcga gagtacacca ttaatgtcct ctcggagagc gtaggagtac
     1621 tctcggtacc ctatttgctc gaaattcttg acggtcattt taacgaccga atacgtgcca
     1681 atgcggtcga agtccttggg cgccaggcca caaacagcaa agactcggag ctgcagaact
     1741 ttctcagtcg ctaccttgat cctcaaacta atcgtcgtgt tcgcgccaat gcgatagtgg
     1801 cgttgcatgc aaatccacgc taccgcgatc gttgtgagcg ggaaatcgaa gctctatcgg
     1861 agtcaaaaga ccgcttcgat cgaggtgctg ctgcgttcgt tattggcgct acctccgacg
     1921 cattaatctc gacggcgttt cgtttcggtc gccaaactca tatttaccga gaaagaattt
     1981 ggggttggag tgctgcccac ggacataaac aaatgtccat gctcatcgcc cttttgcgac
     2041 tgggcgacct tcgagctctg cctcacttcg taaatctttt aatagccgtg cccggcgaaa
     2101 gcccgggggg cctcagggaa gaagccgttt caaatttctc gaccctgcgc gaatcacgac
     2161 gtttgcgggt attagaatac ctctgtgcca atcacccctc agaagttcgc gaagtcacag
     2221 tgttgctgcg tacaagtggt cgcccgttta aaactgattt acattatctg gaagagtttc
     2281 tttccgaaca cgacggcgca attaagctat ctaacattaa agctgcttaa ttccgtgtca
     2341 tagattaata ccaacaccca gcgtaataga actagttgcg gtttttactc tatcgatgtc
     2401 gatggccaac aacgcgatgt ctaaactaat ggccaaagcc cgcttgttct tcattagata
     2461 agcgtaggta atacccgtgg caagctcacg attcgaaagc gataaaacac ttaaattgtt
     2521 agtcacgggt gaatacttgc cgtaaatcat gcctgagtcg ccatttttaa acgagcgatg
     2581 cagcacgggg aacaattgcg ggcccaccat gtctttgaat ccaaacgcat tattagacac
     2641 gaacatgcga gtataataaa agccggttaa caaacttaaa cgccatggtc gtttaattcc
     2701 aggaggcgcg taaccgattc gaccatttac tccaagaaat ctggccgaat tggtgcttgg
     2761 attctgagta agcggcatca aagtaaaata cattgaaccg ccaacatccc acctagcgcg
     2821 gattggataa cgggtagaaa tttttccggt tagcgcgatt gcagaataat tcgaaacccc
     2881 ggtttcttta tactgaatcg acgaaatacc gatgctcggc gtggtgtaaa agcgccgtct
     2941 aataaaaaca cgccgtacca tgcgttttct tggaatctgg gaatctggcc gaacagcgtc
     3001 ggcgttaccc gtttctccta aacccgattt tgcatttcgt tgagcatcgg tcgaagtttc
     3061 attcacctcg accattcgat cttcacgatc gactggagcc gccatcatcg attcgtcggc
     3121 gtgagcttgc ggcaacacac accaaaaaag gaggagaaac attacaggca tctagggtat
     3181 tgtctaaaga attggcgcac cggtcaaggg aatcagagaa tcactaggcc gcaaaggact
     3241 tcttttgaaa ctgaacattc ggaggaactg caccgaaaac gatggtcacg tcatcggcaa
     3301 ggtttgccgc gttcggtccg aaaaacgcca tcgtgtcggc catgaagtct tcacaaatag
     3361 ccggagcatt cgacgcgtga cgctcatgca gacttttgac cattttcacg agggagtttc
     3421 gactgatggt tttaccttcc ggattggtgc attccagcac accatcggta taccaaagaa
     3481 tgacatcacc cggctccaag gcaacggtag ccggctctac ttgaatattt aggtcgtgcc
     3541 cgagtgggtt gcaacgagcg tccatcaaag gcttaatttt ttcttggcgg taaatatagg
     3601 gtggtcggtg ggatgcgttt acaaaatcca tt
//
LOCUS       DAOMXO010000183         3618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14119_length_3618_cov_4.199832, whole genome shotgun sequence.
ACCESSION   DAOMXO010000183 DAOMXO010000000
VERSION     DAOMXO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14119_length_3618_cov_4.199832"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLH57_04960"
     CDS             complement(<1..605)
                     /locus_tag="PLH57_04960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK01993.1"
                     /translation="MKALVVHVPYQVRGGEDVAVESQKKALEALGYEVSLWPNDRTPP
                     KLDPIRILASLNGSDQWKELENEFARERPKFAILNNIYPIYGPRLLRFLNQKKIPLFF
                     AIHNHRLYCTNGLAMRDGSVCKLCKNSSPFRSVAFNCNQNYMRSIYYATALSQIRRDR
                     LLGSLQAFVAPSPYIGEEIKSLGIPMEKIHWIVNPVLDVSGG"
     gene            complement(602..1807)
                     /locus_tag="PLH57_04965"
     CDS             complement(602..1807)
                     /locus_tag="PLH57_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK01994.1"
                     /translation="MTGTADVNKVIQWLYFAILPIVFFVPPIPIGTRKLFLFDLICIL
                     LVGILAVRNWRFLLKQPQKILVLALIGTTLLAWWHGSYRPVELANFELNTVVTLTERP
                     IFQPLRELVIGFRFVVWAMLAGLTIHFLESLSEGSLKTFRTRLLKIAIGCFSLALLVA
                     LLGKVIPGFRTALGYFYQYNTVVIEWRYRSFGVFPSPVEAGAVYGLLGISFFFASSLS
                     FPIRCILTGAAAVGIFLTGSLTPFLAFSTTLGLLVASRLQKTKGFLPVLVLVASLLSA
                     AAFFVISHEGIVKFANFLHRLKVWQSYYGILTSSPQFALLGVGFWPVHADNSVIWFWS
                     RYGLFGIGLFCWFFGKRYFSMIRSPSRGVRYLAVFGTIAALTTDFWVFRPVIAICIAL
                     CLPLFARRS"
     gene            complement(1791..2588)
                     /locus_tag="PLH57_04970"
     CDS             complement(1791..2588)
                     /locus_tag="PLH57_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRK01995.1"
                     /translation="MESTAITQKTPVLSSNISVFNLPESVRQIAQLARGTGVHYVCVS
                     NVHTVIMGERDPVFRKVTNSATLATADGMPLLWASRVLGKSPIHGRASGPDIMKLFFS
                     DPEFADLRHFLFGSTERVLSGLQRNLQTTKNGHNIVGTYSPPMRPVPDAELAISPEEK
                     LECERINQSGANIVWVSLGAPKQEIWMYRMRPHLKAQVLIGVGAAFDFLSGNVKRAPE
                     WMQKSGLEWLYRWTQQPGRLFWRYASTNPEFLVRVAVQALRRGDGNG"
     gene            complement(2607..3542)
                     /gene="lpxC"
                     /locus_tag="PLH57_04975"
     CDS             complement(2607..3542)
                     /gene="lpxC"
                     /locus_tag="PLH57_04975"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRK01996.1"
                     /translation="MGVWNTQTTLRRRVTLKGVGLHSGAMTKCVIHPAPANSGIVFVR
                     SDLKNAPVVQADISKVIRTDMSTTLGVNGVMIATVEHLMAAFYGMGITNALVEIDGPE
                     VPIMDGSSREFADAFEDAGIRSLGVAQKVLRLRRNISVQAGDKYIDAKPSNGLVIRGK
                     ISFKHGVIGEQVYSFKSGKSFREEIAAARTFGFLREVEYLHKKGLAKGGSLENAVVLD
                     DTRVLNPEGLRFNDEFVRHKVLDALGDFALLGLSLQADVEVHKSGHELHTGFIRKVLE
                     DVSNYEIVEAQAQPSRHEHAELIEEEISPLVAAAV"
BASE COUNT         1071 a          917 c          785 g          845 t
ORIGIN      
        1 cctcccgaaa catccaatac cggattcaca atccaatgaa ttttttccat cggaatgccc
       61 aatgatttta tctcttcacc aatgtaggga gaaggtgcga cgaacgcctg taacgaaccc
      121 aataaccgat ctcgcctaat ttgcgaaagt gcggtcgcat aatatattga ccgcatgtaa
      181 ttctggttac aattaaaagc tacactgcga aacggtgaac tatttttaca tagtttgcat
      241 accgagccgt cgcgcattgc caacccattg gtgcaataca aacgatggtt atgtatcgca
      301 aaaaaaagcg gtattttttt ttggttcaga aatcgaagca agcgtggtcc gtaaatcgga
      361 taaatattgt tgagtatcgc aaatttcggg cgctctcgcg cgaactcatt ttcaagttcc
      421 ttccattgat ctgaaccatt cagagaagcc aaaattcgaa tcggatctaa ttttgggggg
      481 gttcggtcat taggccacag cgacacttcg taccccaggg cttcgagtgc ctttttctga
      541 ctctcgacgg cgacatcctc accaccccgc acctgatagg gtacgtgcac caccagagcc
      601 ttcatgatcg cctcgcaaaa agcggaagac aaagggcaat acatattgcg atgaccggac
      661 gaaacaccca aaaatcggtg gtgagcgcgg caatcgtgcc gaacactgcg agataccgaa
      721 ccccccggct gggcgaccta atcatcgaga aatagcgctt cccaaagaac cagcaaaaaa
      781 gtccgatgcc gaacaggccg taccggctcc aaaaccaaat aactgaattg tcggcatgaa
      841 ctggccaaaa gccaacgccc aagagcgcaa actgtggcga cgaagttaat attccgtaat
      901 aggactgcca cacttttaag cgatgtaaga aattcgcaaa cttcacaatt ccctcgtggg
      961 aaatcacgaa aaacgccgcg gctgataaaa gactagccac caaaaccaga accggtaaaa
     1021 agcctttcgt tttttgcaaa cgactcgcca ctaaaagtcc gagagtagtg gaaaacgcta
     1081 aaaaaggcgt gagtgaaccg gttaaaaaaa ttccaaccgc tgccgcaccc gtaagaatac
     1141 accgaattgg aaaactcaga ctcgacgcaa agaaaaagga aatcccaagt aatccgtaaa
     1201 ctgcccccgc ttctacgggc gatggaaaaa caccaaacga acggtagcgc cattctatga
     1261 ccaccgtgtt gtactgataa aaatacccca gtgcggtgcg gaaccctgga attacttttc
     1321 caagtaaggc tactaaaagc gctaatgaaa aacatcctat tgcaattttt aaaaggcgag
     1381 tccgaaaagt ttttaaggaa ccctcactaa gcgattctag aaaatgaatt gtgagtccgg
     1441 caagcatagc ccaaacaaca aacctaaagc caatgactag ttcccgcagt ggctgaaaaa
     1501 taggccgttc ggtaagggtc accacagtgt ttagttcaaa gttagcaagc tcgaccggtc
     1561 gatagctccc gtgccaccac gccaaaagcg tagttccaat caaagcaaga actaaaattt
     1621 tctgaggttg ttttaaaaga aatcgccaat tccttacagc gagaatccca actaaaagaa
     1681 tgcagatcag atcaaataga aataattttc gagtgccaat gggaattggt ggaacaaaaa
     1741 aaacaatcgg caagatagca aaatacaacc attgaataac tttattcacg tcagccgttc
     1801 ccgtcacctc tccgcagtgc ctggaccgct acgcggacca aaaactcagg attagtacta
     1861 gcgtaacgcc aaaatagccg tcccggctgt tgcgtccaac gataaagcca ttccaaacct
     1921 gatttttgca tccactctgg tgctcgcttc acgttgcccg acaagaaatc aaacgccgca
     1981 cccacgccaa tcaatacttg ggccttaaga tgagggcgca tgcgatacat ccaaatttct
     2041 tgttttggcg cacccaaact aacccaaacg atgttcgccc cactttgatt aatgcgttca
     2101 cattccagtt tttcttcggg agaaatcgct agctctgcgt ctggcaccgg ccgcatgggc
     2161 ggagaataag ttccgactat attatgacca ttttttgtgg tttggagatt tcgttggagg
     2221 ccgctcaaaa ccctttcggt tgaaccaaaa agaaaatgtc taagatcggc gaattcaggg
     2281 tcggaaaaaa atagtttcat gatgtcggga ccactggccc tgccatgaat gggactttta
     2341 ccgagaactc ttgaagccca aagcagtggc attccatccg cagtggcgag ggtggccgaa
     2401 tttgtaactt tgcgaaaaac cggatcacgt tcgcccatga tcacggtatg aacattcgaa
     2461 acacatacat agtgtacgcc agttccgcga gcaagctgtg cgatctgcct tacagattcc
     2521 ggtaaattaa aaacggatat gttcgacgaa agaacgggtg ttttctgggt gattgcagtg
     2581 gattccaccg ggcaacctta ccgtgatcaa acggcggcgg caacaagagg tgagatttcc
     2641 tcttcgatca attccgcatg ttcgtgacgc gaaggttgcg cctgagcttc aacgatttcg
     2701 taattcgaaa cgtcctcgag aacctttcgt ataaagccgg tatgcagttc gtgtcccgac
     2761 ttgtgcactt cgacatcggc ctgcaaactc aaacccaata gcgcaaagtc acccaacgca
     2821 tctagtactt tatgacgaac gaattcgtca ttaaatcgta ggccctctgg attaagtacg
     2881 cgggtatcgt cgagcactac agcgttttcc aaggagccac ccttggcgag gcccttttta
     2941 tgcaagtact ctacttcccg caaaaaacca aaagtacgag ccgcggctat ttcttcgcga
     3001 aaactcttac ctgatttaaa tgaatagact tgttcgccaa taacgccgtg tttgaacgaa
     3061 attttaccgc gaatcaccaa accgttcgaa ggcttagcgt cgatgtactt gtctcctgct
     3121 tgcacactaa tgtttcggcg cagccgcagt actttttggg caactcccaa tgagcgaatg
     3181 ccggcatctt cgaatgcgtc ggcgaattca cgagaactgc catccatgat cggaacttcg
     3241 ggaccatcga tttcgaccaa cgcgttggtg attcccattc cgtagaaagc tgccatcaaa
     3301 tgctcgacgg tagcaatcat cactccatta acgccaagcg ttgtggacat gtcagttcga
     3361 attacttttg aaatatcggc ctgaactacc ggggcgtttt tgaggtcaga acgaacaaaa
     3421 acgattcctg aattagcagg agccggatga ataacgcatt tcgtcatagc gccagaatga
     3481 agccctacgc ctttgagtgt cactcgtctt cgtaatgttg tttgtgtgtt ccacacgccc
     3541 atgatcacct atatcgcaac acttatgcca caatagtgga gtatttataa cagattaacc
     3601 cttaacaaaa tcatattc
//
LOCUS       DAOMXO010000184         3612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14152_length_3612_cov_5.694968, whole genome shotgun sequence.
ACCESSION   DAOMXO010000184 DAOMXO010000000
VERSION     DAOMXO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14152_length_3612_cov_5.694968"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1180)
                     /locus_tag="PLH57_04980"
     CDS             complement(182..1180)
                     /locus_tag="PLH57_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK01997.1"
                     /translation="MRSGTIQSMDSERGDLGRVSILNVGTKILLKDVMDQYLRTLGDF
                     KTYYAPNMSSALRVFQENTIQVILTEVDLGDGSAYRLIKELGGDNGLNDLYVILALED
                     RSDALIALATEIEAHAIIVKPFSAQDLKAQIDRFTAWRVMPKEPWLTLIEEARLAARD
                     RRYRDAEVNFVEAMKLAPTNPVPFYKAGMYYIEKGDASMAETFLKKSVELKANYVPAV
                     AALGRVYLAKHDLVKAEEFLRKAHEISPLNPDRLVEMVRMYIERSVEACKNSLRVDPS
                     ATAPHLLLGKLRALLKDYTGSVQELEKALPTLRDSARLEAQTYIALSRKLGGIAKS"
     gene            1274..3421
                     /locus_tag="PLH57_04985"
     CDS             1274..3421
                     /locus_tag="PLH57_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HRK01998.1"
                     /translation="MSQKEFKPFVSDSAKMPELTAVPVLMGVILGIIFGASSLYLALK
                     VGMTVSASIPVAVLSITLFRAFSKIFGVRQATILENNVVQTTGSAGESIAFGVAVTMP
                     ALLILGYDMSLDKIMLVAVLGGLLGVLMMIPLRNALIVKEHGNLIYPEGTACAEILIA
                     GERGGTSAKTVFTGFFVGLAYKFLNVATKLWVDVPERMFTFFRGSYVATEVSPELLGV
                     GYIIGTRTACIMVAGGILSTWVLIPAITLFGSGLSAPLFPATKLISQMGPHEIWKNYV
                     LYIGAGAVAAGGIISLVQSLPTIVRGAVAGLGDLKASRSGSKTTARTNRDLSMKWVVL
                     GSAALVAGIWATPSLHMNLLGALLIIVFGFLFVTVSSRLTGQIGSSSNPISGMTVATL
                     LLTSLIFLLLGWVEADYRVTALCVAAIVCVAASNGGTTSQDLKTGYIVGATPRAQQIG
                     LLFGVTTSALVVGFTLNLLNDASTVYSRKDLPAVQIQDLSKLKDKEKAQGMKDDQLYH
                     VLRVTELSSTGPLAGVAAGKYLVDGNGYIKYLVDPGINGTLRQRDDGTPVQKYDAPKA
                     RLMSLIIDGILAQKLPWGLVLLGAAISIVLELCGVASLAFAVGVYLPLAASAPIFFGG
                     AVRWLVERSTKKKKASLVEEETSPGVLMSSGLIAGGSIAGILIAMSSLWGDIGQKLDL
                     SQLAPAITGSNVTAVVMFSLLMLLLYRTGKSKS"
BASE COUNT          815 a          919 c          900 g          978 t
ORIGIN      
        1 gcgcatataa agtggactag gaaatgtagc gccggcgccg ttgattagag ctgcgccatt
       61 ggcgctgcca actgcagaaa aaaaagcgac cgaaaaaata cacaatttta gaagtttcat
      121 tttggaatgc ctccgccacc tttgtagcaa agggtccaaa ctattcgttg ttaagattcg
      181 gttaggactt ggctattccg ccaagcttcc gagacagcgc aatgtaggtc tgggcttcga
      241 gcctcgcgga atcccttagg gtaggaaggg ctttttcaag ttcttgtacg ctgcccgtgt
      301 agtctttcag cagagcccgc aatttcccca ataacaaatg gggcgccgtt gcgctgggat
      361 ccactcttag tgagtttttg caggcctcga ccgagcgttc aatatacatt cggaccatct
      421 cgaccaagcg atcaggattt aaaggggaaa tctcatgagc ttttctgagg aactcttctg
      481 cttttaccaa gtcgtgtttg gccaaataga cccgtccaag tgcggctaca gccggaacat
      541 agttggcctt tagttcgact gattttttga gaaaggtctc tgccatgctg gcatcgcctt
      601 tttcgatgta atacattccc gccttataaa atgggaccgg attagtggga gccaacttca
      661 tggcctcaac aaagttcact tcagcgtcgc ggtagcggcg atctcgtgcc gctagccggg
      721 cctcttctat taaggttaac caaggctcct tgggcattac ccgccacgcg gtaaaacgat
      781 cgatctgcgc tttgagatcc tgggcggaaa agggctttac gataatagcg tgggcttcaa
      841 tctcggtggc cagggcgatg agagcatcgg atcgatcctc caatgccaaa atgacgtata
      901 agtcgtttaa gccattatct ccgccgagtt ccttaatgag gcgataggcg ctaccgtctc
      961 ccagatcgac ttcggttaaa atgacttgaa tggtgttttc ctgaaatacc cgaagcgccg
     1021 aactcatgtt cggagcatag taggtcttaa agtcgcccaa agtacgcagg tattgatcca
     1081 tcacgtcttt caacaagatc tttgtaccga cgtttagtat tgaaacccgc ccaaggtcgc
     1141 cgcgttcgct gtccattgat tgaattgtgc cagacctcaa aaattttgca ccccctatag
     1201 gttttttgtc gcacctcgtc tatagaagaa aaaacattcc gtttatctcg tgtataggaa
     1261 ggggccccaa caaatgagtc agaaagaatt taagccattc gttagtgatt ccgcaaagat
     1321 gccagagtta accgcggtgc ccgtcctcat gggcgttatc ctgggcataa ttttcggagc
     1381 ctcgtcgctc tatttagctc tcaaggtagg catgaccgtc agcgcctcga ttcctgtggc
     1441 cgtgctttcg attactttat ttcgggcctt ctcaaaaatt ttcggtgttc gccaagctac
     1501 tattcttgaa aacaacgtag tgcaaacaac tgggtctgct ggcgagtcga tcgcatttgg
     1561 cgttgccgtc accatgcccg cgctcttgat tcttggttac gacatgagcc tcgacaaaat
     1621 catgctcgtc gccgtcctag gcggtttgtt gggcgttctc atgatgattc ctcttcgcaa
     1681 tgcactcata gttaaagaac acggaaatct aatttatccg gaaggcactg catgtgcgga
     1741 aattttaatt gccggcgagc gcggtggcac gagcgccaaa accgtattta ccggattttt
     1801 cgtgggcctt gcgtacaaat tcctaaacgt cgccacaaaa ctttgggtcg acgtaccgga
     1861 gagaatgttc accttttttc ggggctccta cgttgccact gaagtttccc ccgaattact
     1921 tggggtcggt tacatcattg gcactcgcac tgcttgcatc atggtggcag gcggtattct
     1981 ttccacttgg gttttaattc ctgcaatcac tttgttcggc agtggattga gcgccccact
     2041 gtttccagcg actaagctaa tttcccaaat ggggcctcac gagatttgga aaaattacgt
     2101 cctttatatc ggcgctggcg ccgtcgccgc tggtggcatt atcagtctcg ttcaaagttt
     2161 accaacgatc gtacggggtg cagtagctgg tttgggcgac ctaaaagcgt cgcgctctgg
     2221 cagcaaaaca accgctcgaa ccaaccgcga tctttcgatg aaatgggtgg tcctcgggtc
     2281 cgctgctttg gtggctggaa tctgggcaac accgtcgttg cacatgaacc tactcggtgc
     2341 gctattgatc atcgtgtttg gtttcttgtt cgttacggta agctctcgcc tcactggcca
     2401 aattgggtcg tcgtcgaatc caatttcggg tatgaccgtt gcaactctgc tccttacaag
     2461 tttgatattc ttgctcctcg gctgggttga agcggactat cgtgtaaccg ctctttgtgt
     2521 ggctgccatt gtctgcgtgg ctgcttcgaa cggcggcacc acgtcccagg acttaaaaac
     2581 tggctacatt gtgggtgcca cgccgcgagc tcaacaaatc ggattgttgt tcggcgttac
     2641 tacttcagcg ctcgttgttg gatttacact caacttgtta aacgatgctt caacagtata
     2701 ttcacgtaaa gatttaccag cagtacagat tcaagatctc tcgaagctga aagacaaaga
     2761 aaaagctcag ggaatgaaag atgatcaact gtatcatgta ctgcgtgtaa ctgaactctc
     2821 gagcactggc ccacttgctg gcgttgctgc tggcaaatac ctggtcgacg gcaatggtta
     2881 cataaagtac ttggtcgacc caggcatcaa cggcacactt cgccaacgcg atgacggtac
     2941 gccagtgcaa aagtacgatg ctcctaaggc gcgcttgatg tcgcttatta tcgacggcat
     3001 tcttgctcag aaattgccat ggggtctggt gcttttaggt gccgctattt ctatcgtact
     3061 cgaactttgc ggagtagcct cgctggcatt cgcagttggc gtgtacttgc cacttgccgc
     3121 atcggcgccg atcttctttg gcggagcggt tcgctggttg gttgaacgct ccactaagaa
     3181 aaagaaagcc tcgctcgttg aagaagagac gagcccagga gttcttatgt cgtcgggact
     3241 tattgctggt ggctccatag ccggcatttt aattgccatg tcgtcacttt ggggcgacat
     3301 cggtcaaaag cttgacctca gtcaactagc tcccgccatt actggatcga acgttaccgc
     3361 cgtggtgatg ttcagcttgt taatgctcct cttataccga acgggtaaga gcaagagcta
     3421 aaagtccttc atatataacg ctctctaaac gggctgcggc gctagcgcct cgcagcccgt
     3481 ttttttattc gttcctcgta actttcgtcc tacatggaat cattacaacg acggatcgat
     3541 tggattccaa tacccgtccg agacatccgc cgcattggct tggtgcgttg cggtatgcgg
     3601 ccccgcatcc ca
//
LOCUS       DAOMXO010000185         3612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14153_length_3612_cov_5.625527, whole genome shotgun sequence.
ACCESSION   DAOMXO010000185 DAOMXO010000000
VERSION     DAOMXO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14153_length_3612_cov_5.625527"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLH57_04990"
     CDS             <1..287
                     /locus_tag="PLH57_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK01999.1"
                     /translation="EDAARHFETMFQNGFDLPHARNSFALTLLDLNRKSEAFEQFRQA
                     IRQDPTFLDARLNLAQFLFLDGQKEEATLHFEMALKIDPTNPVARRYLNR"
     gene            301..1356
                     /locus_tag="PLH57_04995"
     CDS             301..1356
                     /locus_tag="PLH57_04995"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRK02000.1"
                     /translation="MTHHKWLLYGAYGYTGRMITGEALKRGHRPVLAGRSASALQEFA
                     KNFALDTLVFDLDHPNLVNIVRGFNLVFNAAGPFSKTSEPLVRACLEASVNYVDITGE
                     VAVFEKNFSYDAAAKKQGIVILSGVGFDVVPTDCAAKQVCDRVPQATDLEIAFAISTG
                     GSTGTLSTALEAAPQGILERRAGQIVPISHFSDNKRVAFMDRSRTVMPITWGDLATAY
                     RTTGVPNISTYVAVSPAVAAGTRLFGKFVFSLLKNSLVRRLAQWLIKLLIKNPNPTTS
                     WNAKSYVWARARNNEGRQAEVQIELPCGPYPFTAVAGVASVERIFRDRPTGATTPALA
                     FGTDFLSSLPGTKILNT"
     gene            complement(1353..2378)
                     /locus_tag="PLH57_05000"
     CDS             complement(1353..2378)
                     /locus_tag="PLH57_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02001.1"
                     /translation="MAFILQFLLSAGVIIVAGSLLTRFADKIADVTGWGRMFVGSLLL
                     AGATSLPELMVDIKAVRIGLPDLAVGDLLGSSLFNLLILSVLDFAFPSAFRRTAFSPK
                     FLHHSLAAILTIVLTAIVGLGVATRLETSFLGIGVFSWATIIIYLYGLRLIFLDKAEQ
                     DLVNGAPVPEGRAAEPNSGSAKFSSIVGAFSGYFASAFIIVLAAPYLVEAADQIATQS
                     GLGHTFVGTTLVALATSLPELIATLSAFRIGSPDLALGNIFGSNAFNMILFAPLDFMY
                     PGALFSSVRAVHAVTAFSIVIATGVAVMGQLYRKKERSRFAEPSSEGVVVVILMFLFL
                     LYQLNAN"
     gene            complement(2419..2778)
                     /locus_tag="PLH57_05005"
     CDS             complement(2419..2778)
                     /locus_tag="PLH57_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02002.1"
                     /translation="MSKSTRLVYSTDPALNQKCQKCKELKSECTCAPEVDVAKYKFIA
                     VLRIEKQGRGGKTVTVIDQLPKNEIFLKNLTTLLKKKCGSGGTYVMSGREGVIEIQGD
                     KRDMIRVILEKEGIRSK"
     gene            complement(2838..3242)
                     /locus_tag="PLH57_05010"
     CDS             complement(2838..3242)
                     /locus_tag="PLH57_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRK02003.1"
                     /translation="MKNMPRIQKYMTAMPHTIGSDISIKKAIEMMKEFRIRHLPVQQG
                     GKLVGVITDRDIKLASSFQGTDEMLVDDVMTPDPYVVDPDAQIDDVVFNMAEHKYGCA
                     IVQQQNDKVVGIFTDNDGLRVLGEVLRQNYKL"
BASE COUNT          947 a          971 c          816 g          878 t
ORIGIN      
        1 tcgaagatgc tgctcgccat tttgaaacca tgtttcaaaa tggttttgac ctaccacacg
       61 cccgcaatag ttttgcactt accttgttag atctaaacag aaagtcagag gcctttgaac
      121 aattccgcca agcaattagg caagatccga cttttctcga tgcacgtctc aatttggcgc
      181 aatttttatt tttagacggt caaaaggaag aagccactct tcacttcgaa atggcactca
      241 agattgaccc cacaaaccca gtcgcacggc gctatttgaa tcgctgataa agggctctcc
      301 atgacacatc acaaatggtt gctttacgga gcctacggtt acactggtcg catgataact
      361 ggagaggctt taaaacgtgg tcaccgcccc gtgctggctg gacgttctgc gtcggcgctt
      421 caggagtttg ctaaaaattt cgcgctagac accctggttt ttgacctcga tcaccccaat
      481 ctcgtaaaca tcgtaagggg ttttaacctg gtgtttaacg ccgcgggacc attttcaaaa
      541 accagtgaac cactagtgcg cgcctgttta gaggcttccg taaactatgt cgacattacg
      601 ggtgaggtcg cggttttcga aaagaacttt tcctatgatg ccgcggcaaa aaaacaaggc
      661 atcgttattc tttcgggagt gggtttcgac gtcgtaccca cggattgcgc agccaaacag
      721 gtgtgcgacc gagtgcccca ggcaaccgat ttggaaattg ctttcgcaat cagtaccggc
      781 ggtagcactg gcacactgag caccgcgctc gaagcggctc cacaaggcat tctcgaacga
      841 cgagcaggtc aaattgtgcc catttcccat ttctccgata acaaacgcgt cgccttcatg
      901 gaccgttctc gaacggtaat gccgatcacc tggggcgatc tcgctacagc gtatcgcacc
      961 accggagtgc cgaacatcag cacctacgtg gcggtgtccc ccgctgttgc ggcaggtact
     1021 cggctcttcg gaaaatttgt cttttcgtta ttaaaaaatt cgctcgttcg gcgtttagcg
     1081 caatggctca tcaaactctt gataaaaaac ccgaacccca cgacctcatg gaacgccaaa
     1141 tcctacgtgt gggctcgcgc tcggaacaat gaagggcggc aagcggaagt gcaaattgaa
     1201 cttccctgcg ggccctaccc attcactgct gtagctggag tagccagcgt ggaacggatc
     1261 tttcgagatc gccctactgg tgcaaccacg ccggccctgg cctttggcac cgatttttta
     1321 agttctcttc cgggcaccaa aattttgaac acctaattgg cattgagttg atagagtaaa
     1381 aatagaaaca tcaatataac tacaaccacg ccttcggaac tgggctcggc aaaacgtgaa
     1441 cgttcctttt ttcgataaag ttggcccatc acggccactc cggtcgcaat cacgatactg
     1501 aacgcggtca cagcatgaac cgcgcgaacc gaactaaaaa gcgcgccggg gtacataaag
     1561 tccagtgggg cgaacaagat catattgaac gcgttactgc caaaaatatt acccaacgcc
     1621 aaatctggag aacctatgcg aaaggccgaa agcgtcgcga ttaactccgg caatgaagtc
     1681 gcaagcgcga ccaatgttgt acctacaaac gtgtgcccaa gtcccgactg cgtcgcgatt
     1741 tggtcggccg cttccacaag atacggcgcc gccagaacaa taataaatgc cgacgcaaaa
     1801 tacccagaaa aagcccctac gatcgagctg aacttagcgc tgcccgagtt tggctcggcg
     1861 gctcgccctt ctggcaccgg tgctccattc actaaatctt gctcagcctt atctaggaag
     1921 atgagtcgaa ggccgtacaa gtaaatgatg attgtggccc acgaaaacac accgatacca
     1981 agaaacgaag tctctaaccg cgtggctacg ccaaggccaa caatcgctgt taagacaatt
     2041 gttaaaatag cggccaaaga atgatgcaaa aacttgggcg aaaacgcggt acggcgaaac
     2101 gccgaaggaa acgcaaaatc caaaactgat aatataagaa gattaaaaag actactgccc
     2161 aacaaatcac cgacggcgag atccggcagt ccaattcgga cggctttaat atctaccatt
     2221 aactcaggta gcgacgtcgc acctgctaac aacaaactgc caacaaacat tcgcccccag
     2281 cccgtgacat cggcgatctt gtcggcaaaa cgagttaaaa gtgaaccggc gacaataatg
     2341 acgccagcag acaatagaaa ctgaagtatg aatgccattc actgcatttt atacctgtaa
     2401 tcccgcgcgt ggcaaacgtc acttcgagcg aatgccttct ttttccaaga tcactcgaat
     2461 catgtcgcgc ttatcgccct gaatctcgat aacgccttct cggccactca ttacataagt
     2521 gccgcccgaa ccgcattttt tcttgagcag agttgttagg ttcttgagaa atatttcgtt
     2581 cttgggcagc tgatcaatga cggtcaccgt tttgccgcca cgcccctgtt tttcaattct
     2641 taatacagcg atgaatttgt atttcgctac atccacttcg ggcgcacagg tgcactcgga
     2701 tttcagctct ttgcactttt ggcacttttg gttcagcgcc ggatcggtcg aataaacgag
     2761 tcgggtagat ttgctcatta caattccaaa atgaaaccca aacctaccct aaatcaaccc
     2821 gttaggcgac tgagacttta cagtttgtag ttctgacgca gcacttcgcc cagtacccgt
     2881 agaccgtcgt tatcggtgaa aatccctacg accttgtcat tctgttgctg cactatcgcg
     2941 cacccgtatt tgtgttctgc catgttgaac acgacgtcgt cgatctgtgc atctgggtcc
     3001 accacgtagg ggtcgggcgt catcacgtcg tctactagca tttcgtcggt accttgaaac
     3061 gaactcgcaa gtttaatgtc gcgatcggta atgaccccaa ctaacttgcc gccctgttgc
     3121 acgggtaggt gccgaatgcg aaactccttc atcatctcaa tcgccttttt gatcgaaata
     3181 tccgagccga tcgtatgtgg cattgccgtc atgtattttt gtatgcgtgg catgtttttc
     3241 atcttggttc tccttctact gaaatgaaat taggtaccgc tgagtattgc aactggcgtt
     3301 ccaaacacat tccttaggca aacctgggcg attagcgccg aaagttcgtt ttttttgggt
     3361 caatttgcct caatattgct gaatctgtct cagcccgacc ccggccacct cggcggattt
     3421 ccgacctaat gattttggca cgctccgtgc tttaagctaa accatgacaa cgaatccgct
     3481 taaaccccct atttcggacg tacaaaatta cgcactacga gaaaaaatgt cccatgatat
     3541 agggttttcc atgaaaggtc ataaaggaca tatcgtgatc atcgacgatg atcgcgaaat
     3601 gcagtcgcta tt
//
LOCUS       DAOMXO010000186         3570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14380_length_3570_cov_4.621337, whole genome shotgun sequence.
ACCESSION   DAOMXO010000186 DAOMXO010000000
VERSION     DAOMXO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14380_length_3570_cov_4.621337"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PLH57_05015"
     CDS             complement(<1..660)
                     /locus_tag="PLH57_05015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK02004.1"
                     /translation="MNTKPRPLLTTYAAVVFAFLYLPLVVMAVASIGGPQGLFSSYQR
                     AFADPALMLSLKNSVLVGIGTTILSTALGVTAALALRRKSFPGRLVFESLLNLPLVLP
                     EIVMGLALLIWFVFLKISLGLVSVVLAHTTFTVSYVTLILMARLKGMDPLIEEAAQDL
                     GATPIRTFFHITFPLLLPAIVSGALLAFTLSFDDFLLTFFTAGVGSDTLPVRIYSMLR
                     YG"
     gene            complement(657..1541)
                     /locus_tag="PLH57_05020"
     CDS             complement(657..1541)
                     /locus_tag="PLH57_05020"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK02005.1"
                     /translation="MNHATPASVPLSPSPKKKFPLMLVPSALWFAIFLIIPILIVMSL
                     SLLHKGSYGRIEFTLDFSNYARALEPVYLKIILRSLWLATYTTGLCLLLAYPLAYLMA
                     RSRPRTRKILLALVVVPSWTNFVIRIHALKLIIGETGLLNQILTELSIITRPLQMTDN
                     AFGVGMGMVYNYLPFMVLPLFVALEKLDFTLLDAAYDLGASRLQSFFRVLFPLSMPGI
                     VTGSLFVFIPAFGEFVIPDMLGGSQSMYVGNIITETFLKSRDWPFGSALSTFLVLLAM
                     VAFMIVLSRENKTQGAKF"
     gene            complement(1538..2659)
                     /locus_tag="PLH57_05025"
     CDS             complement(1538..2659)
                     /locus_tag="PLH57_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK02006.1"
                     /translation="MQVRKGEFFSVLGPSGCGKTTTLRMISGLETPTSGTIRMSGRVV
                     NGVHPHKRNVHTVFQKYALFPHLNVFENVAFGLRMKKVSSEQIKRRVNRMLEMVRLET
                     YADRMIQQLSGGEQQRVALVRALVNEPEILLLDEPLGALDLKLREQMQVELVAIQKQL
                     GTTFIFVTHDQGEALTLSDRIAIMNRGRIEQIGTPTEVYEHPASIFVAKFVGTSNVFE
                     GTSEFESNSQGASFVAIQTSLGRLRAKFHNGNTRFHPRDVPSAALIVRPEKIRVRVTS
                     PSGESGSADLNSVRGTLVERTYFGPTTQIKLKINDQAAPLIALIANQASVKAPTLKLG
                     EKYYAVWDPGDSIIVPSDARVAAALDAPISQPLGSTATA"
     gene            complement(2803..>3570)
                     /locus_tag="PLH57_05030"
     CDS             complement(2803..>3570)
                     /locus_tag="PLH57_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02007.1"
                     /translation="ISTYAREGWFKVRTTKGDYGWVWQADVVVPTSKTDSKAAQMDFI
                     PRPETKRKDRVAPWLFVRAGPKAVASLNGGLSNKLGLGDNHFYPHLGGYADIAFSLEE
                     SIRLAFRTEFLSMSQSVSYFTVNYDLSQSSTLFFVGLDVDVVRGESWDLGAAAYFGGN
                     SSSLTVKCTSYVAPNSFTVDHFNYAALIQFTSRYWFNQRLAFVGELGAHIAPAPRSQI
                     QANFNGSEPFRNPDGDVGQVKASASGPYISAALQLAF"
BASE COUNT         1012 a          832 c          926 g          800 t
ORIGIN      
        1 accgtatcta agcatcgagt aaatacggac tgggagcgtg tcgcttccga cccccgccgt
       61 aaagaaagtt aataaaaagt cgtcgaacga aagggtaaag gcaagcagcg ccccgcttac
      121 aatggctggc agtaacagcg gaaaggtaat gtgaaaaaaa gttcgaatag gcgtggcgcc
      181 taggtcttga gcggcctctt cgattagagg gtccatgcct ttgaggcggg ccattaagat
      241 aagtgtaacg tacgaaaccg taaaagtagt gtgagccaaa acgactgaca ctaatcctag
      301 ggaaattttt agaaacacaa accaaattaa cagcgctagt cccatgacga tttcgggtag
      361 aacgagaggg aggttaagaa gtgattcaaa aaccagacga cctggaaagg attttctcct
      421 taatgccaac gcggcggtca ccccaagtgc cgttgaaagt atggtggttc cgataccaac
      481 aagcacgctg ttcttgagcg aaagcatgag cgcagggtcc gcaaacgccc tttgatacga
      541 tgaaaataag ccctgtggtc ccccaatcga tgcgaccgcc attacgacta agggaagata
      601 caaaaatgca aagactacag ccgcgtaggt ggtgagtaga gggcggggtt tcgtgttcaa
      661 aacttagctc cttgagtttt gttttcacga ctcaatacga tcataaatgc caccattgcg
      721 agcaaaacaa gaaacgtact gagcgcgcta ccaaacggcc agtctcgcga tttaagaaaa
      781 gtttccgtaa taatatttcc cacatacatg ctctgggagc cacccaacat gtcgggaatc
      841 acgaactcgc caaaggccgg aataaaaaca aaaagactac cggtgacgat cccaggcata
      901 ctcaggggaa acagaactct aaagaaactt tgaagccgac tagcgccaag gtcgtaggca
      961 gcatcgagta aagtaaaatc aagcttttcg agagcgacaa atagcgggag caccataaac
     1021 ggtagatagt tataaaccat gcccatacca acaccaaaag cattgtccgt catttgcaat
     1081 ggccgagtta tgattgagag ttcggttagt atttggttga gaagaccggt ttcaccgata
     1141 attagtttaa gcgcgtgaat gcgaatcaca aaattggtcc aagaaggaac tacgaccaaa
     1201 gcgagtaaaa ttttacgtgt tcgcggccgt gatcgagcca tgaggtaggc taaaggatag
     1261 gccaaaagta ggcaaagccc agtagtgtaa gtcgcaagcc acaaagaacg gagaatgatt
     1321 tttaaataaa ccggctcaag agctcgcgca taattcgaaa agtctaaggt aaattcgatg
     1381 cgtccgtagg agcctttatg cagaaggctc aaagacatga cgattaaaat tggtataatc
     1441 aaaaaaatcg cgaaccaaag cgcgcttggg accaacatga gcgggaactt tttctttgga
     1501 gaaggcgaaa gtggtacaga ggcgggtgtg gcgtggttca tgcggtagcc gtggaaccca
     1561 agggttggga aatgggcgcg tcgagtgccg cggcgaccct ggcgtcggag ggaacaataa
     1621 tgctgtctcc ggggtcccag accgcgtaat acttttcacc aagttttagt gtgggcgctt
     1681 tgacagaggc ctggttcgcg atgagtgcga tcagcggtgc cgcctggtcg tttattttaa
     1741 gttttatctg cgtggttggt ccaaaatagg tgcgctccac gagggtgccg cgcactgaat
     1801 ttagatcggc cgaacccgat tccccagacg gagaagtgac tcgcacccgg attttttccg
     1861 gacgcacgat caacgctgcg cttggaacat cacgaggatg aaaacgagtg ttcccgttgt
     1921 gaaacttcgc ccgcaaacga ccaagcgagg tttgtattgc tacgaaggaa gccccttggg
     1981 aattactctc aaactctgaa gtgccctcga atacattcga agtgcctacg aacttcgcca
     2041 caaatataga agcagggtgc tcgtacactt cggtgggtgt tccgatttgt tcaatgcgac
     2101 cccgattcat gatggcgatg cgatcggaaa gtgtaagagc ctcgccttgg tcatgggtta
     2161 cgaaaatgaa ggttgttcca agctgtttct gaatagcaac aagttcaacc tgcatttgct
     2221 cccgtagttt aagatcaagg gcaccgagcg gttcgtctaa aagaagaatt tcgggttcat
     2281 tgaccaatgc tcgcactagc gcaacgcgtt gttgttcacc acccgagagc tgctggatca
     2341 tgcggtcggc ataggtttca agtcgcacca tttccaacat gcgattcacg cgccgtttta
     2401 tttgttcgct cgagactttt ttcatgcgaa ggccaaaggc gacgttttcg aaaacattga
     2461 gatggggaaa caaagcgtat ttttgaaaaa ccgtatgaac gtttcgtttg tgcgggtgaa
     2521 cgccgttgac caccctcccc gacatgcgaa tggtaccgct ggtcggagtt tcaagaccac
     2581 taatcatgcg aagggtagtt gtttttccac aaccgcttgg tccgagaacc gaaaaaaact
     2641 cgccttttcg cacctgcatg gaaagcgaat cgaccacgac gtttatagcc gaggacttat
     2701 ccgagttcgc agcgaaactc ttggagacat tctcgaaatc tagcgcgagc gtttcggtca
     2761 tagtgaggcc gacgtttatc acaaacgacc agatggtcca gcttaaaacg cgagctgaag
     2821 cgctgcgctg atataaggcc ccgaagccga ggctttgact tgacccacat cgccatcagg
     2881 atttctaaaa ggctcagatc cgttgaagtt tgcttgaatc tgggagcgag gagctggagc
     2941 tatgtgtgcg ccgagttcgc ccacaaaagc caagcgttgg ttgaaccagt atcgactcgt
     3001 gaattgaatc aatgcagcat aattgaagtg atccaccgta aagctattgg gcgccacata
     3061 acttgtgcac tttacggtta gggaactgga gtttccgcca aaatacgcgg cagcacccaa
     3121 atcccagctt tcgccgcgca ctacatcgac atccaaaccg acgaaaaaga gagtagatga
     3181 ttgggatagg tcgtaattca ccgtaaaata gctaacggac tgcgacatcg aaagaaattc
     3241 agttcgaaat gccagtcgaa tagactcttc gagcgaaaaa gcgatatcgg cgtagccgcc
     3301 taaatgagga taaaagtgat tgtcacccaa accaagtttg ttgcttaaac caccgtttaa
     3361 actggctact gctttaggcc ctgctcgtac gaacaaccag ggagctacgc ggtctttgcg
     3421 cttggtttcg ggtcggggaa tgaaatccat ctgggcggcc ttagagtctg ttttggaggt
     3481 tggtaccact acgtccgctt gccataccca accgtagtcg cccttcgttg tacgaacttt
     3541 aaaccagcct tcgcgggcgt aagtagaaat
//
LOCUS       DAOMXO010000187         3560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14445_length_3560_cov_4.381455, whole genome shotgun sequence.
ACCESSION   DAOMXO010000187 DAOMXO010000000
VERSION     DAOMXO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14445_length_3560_cov_4.381455"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..400)
                     /locus_tag="PLH57_05035"
     CDS             complement(80..400)
                     /locus_tag="PLH57_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRK02008.1"
                     /translation="MTKADIIEALYEKIGYSKKDAADLVELIFDTIKDTLSRGEKIKI
                     SGFGNFVVREKRPRTGRNPQTGEAIKITARRVLTFKPSQVLRADVNNSTKPGSGNSGE
                     SNGN"
     gene            558..1202
                     /locus_tag="PLH57_05040"
     CDS             558..1202
                     /locus_tag="PLH57_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02009.1"
                     /translation="MSRFKTFILTLLLSCYAASAAAVEMVEMWAFMSGSAITRFQWSA
                     TESQIQEWIKEDQATVAAISRSLERLSEIEPKGSLKGLGIDGKSRAMQNYILLDLKRA
                     NDALKAISKLEDRIDETDNLAMRDPEYKMQAERLRTARAEQIEIVEAEVRKALVHYMV
                     ILKLAASADEKFPFSLSVLEAESQNKSLKLENSLEKILIRFTRPKEQIRCKHLL"
     gene            1249..3303
                     /gene="acs"
                     /locus_tag="PLH57_05045"
     CDS             1249..3303
                     /gene="acs"
                     /locus_tag="PLH57_05045"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015116355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRK02010.1"
                     /translation="MRTTVPRSNIDVHLKETRLFKPDRAFSKATGLDLKTYKKWLRSG
                     KATPEKYWEAAAQELTWHKKWKKTLKWTPPHAQWFVGGKLNGAENCLDRHLPLHGNKA
                     ALVWEGEPGEVFTWTYRQLHREVEKFTALLRNTGLKPGDRVAIYMPLVMESIVAMLAC
                     ARGGFTHTVIFGGFGAEALKDRINDAAAKVVVTADYGWRKGLQVKLREQVDAELKNCP
                     TVEHVVLLERAFSGRMTDGFSESLGRGTRVSQPKHLGRATVLLWHDALAAVSETQRKN
                     FGGIESVDAEHPLFILYTSGTTGKPKGLVHSTGGYLAGALRTAKIVFDLKLTDVYWCT
                     ADIGWITGHSYVVYGPLAAAGTVFLYEGAPTHPTPDRFWNMIARHRVSIFYTAPTAIR
                     SFMRLGNELPKKHDLSSLRVLGSVGEPINPEAWMWYHDIIGGGRCPIVDTYWQTETGA
                     IVISPLPGFIATKPGSATRPLPGYDVDVVDKAGKSVKNGTGGYLVIRKPWPSMARTIH
                     GDPERFKKQYFSEFKGGVYFTGDGARRDRDGYIWCLGRVDDVLNVSGHRLGTMEVESA
                     LVSHPAVAEAAVVGRPDELKGQGIVAFVTLKASHAAQLRGNLSEKAQFESLLKGHVVK
                     QIGALARPDEIRFTKNLPKTRSGKIMRRLLRELATNGTVQGDTTTLEDLSVIAALQKD
                     EE"
     gene            3320..>3560
                     /locus_tag="PLH57_05050"
     CDS             3320..>3560
                     /locus_tag="PLH57_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02011.1"
                     /translation="MKALLRLVERSDPNECQRFAQWVNTHYPSRLPLEARTVASPDHF
                     SFWVFAADSGEKGELLGATSFAERTPHLAETMRTVV"
BASE COUNT          860 a          847 c          953 g          900 t
ORIGIN      
        1 cttcacaccc acgatctcag cgacttcgcc gatcttaaag tacatgcggt caggaatgcg
       61 agcttgctca ataacttgac taatttccat tcgattcgcc cgagttgccg gaacctggtt
      121 tggtggaatt attgacgtcc gctcttagaa cctgcgatgg cttaaacgta agaacgcgcc
      181 gcgcggtaat tttgatcgcc tcgccagtct gggggtttcg gccagtacgg gggcgctttt
      241 cgcgaactac gaaattacca aaaccgctga ttttgatttt ttcgccccgc gaaagagtgt
      301 ccttgatcgt gtcaaaaatc agctctacga ggtcggctgc gtccttcttg gaataaccga
      361 tcttttcgta aagggcctcg atgatgtccg ctttggtcat gcttctcatt ctattgtccc
      421 cgtctggtta gcaaaacagc tgagatatca atatgatatc gggaacccgc caaaacgcaa
      481 agaataattt ttctaagccg ccaccgcggt ttttttggct gctttgtggt atgtagctgc
      541 tcggaaaaag gggtattgtg tcgaggttta aaacattcat actgaccctc ctcttgagct
      601 gttacgccgc ttctgctgcg gccgttgaaa tggtagagat gtgggctttc atgagcggaa
      661 gtgcgatcac tcgttttcag tggtcggcca ccgagagtca gattcaagaa tggataaaag
      721 aagatcaggc cactgttgcg gccatttcgc gttctttgga gcgtttatcc gagatcgaac
      781 cgaagggctc gttaaagggg ttgggtatcg atggcaaatc gcgagcaatg cagaactata
      841 ttttgttaga cctcaagcgc gctaacgacg ccttgaaggc gatcagcaag ttagaagatc
      901 gtatcgacga aaccgataat cttgccatga gagatcctga atataaaatg caggccgagc
      961 gtttacgtac cgcgcgggcc gaacagatag aaatagtcga agctgaagtg cgcaaagcgc
     1021 ttgtgcatta catggttatt ttgaaactag cggcgagtgc cgatgaaaaa tttccgtttt
     1081 ctctttccgt ccttgaagca gagagtcaaa ataagagctt aaagctcgag aattctctcg
     1141 aaaaaatctt gattcgattt acccgcccaa aagaacagat tcgttgcaag cacctactgt
     1201 aagtgtaaat atcttcgctc aataccaaac tttcacccga ggaatcagat gcgaacgacc
     1261 gttcctagat cgaacattga cgtgcattta aaagagacgc gtttattcaa gcctgatcga
     1321 gctttctcga aagctaccgg actggaccta aaaacctata aaaagtggtt gcgctcagga
     1381 aaagcaactc ctgaaaaata ctgggaagca gctgcccagg aacttacctg gcacaaaaaa
     1441 tggaaaaaaa ctctcaagtg gaccccgcct catgctcaat ggtttgtggg tggaaaacta
     1501 aatggtgctg aaaactgttt agaccggcat cttcctttgc atggtaataa ggccgccttg
     1561 gtgtgggaag gtgaacccgg tgaagtgttt acgtggacct accgccagct tcatcgcgaa
     1621 gtggaaaagt tcaccgcgct attacgcaac acagggttga agcccggcga ccgagtcgcc
     1681 atttatatgc cgcttgtcat ggaatcgatc gtggccatgt tggcgtgcgc gcgtgggggc
     1741 tttactcaca ctgtaatttt tggtggtttt ggtgcagagg cactcaagga tcgaattaat
     1801 gacgccgcgg ccaaagtggt cgtaacggct gattacggtt ggcgcaaagg tcttcaggta
     1861 aaacttcgtg agcaagtcga tgcggagctt aagaattgcc ccacggtgga gcacgtagtg
     1921 ctgcttgagc gcgctttttc tggccgtatg acagacggtt ttagcgagtc gttggggcgg
     1981 ggaacgcgcg tttcccaacc gaaacatttg gggcgagcga ccgtgttgct ttggcacgac
     2041 gcgctggcag ccgtttccga aacgcagcga aaaaactttg gcggtatcga gtcggttgac
     2101 gctgaacacc cactattcat tctttatacg agcggtacta ccggcaaacc caagggccta
     2161 gttcattcca ctggcggtta tttggccggg gcattgcgta ccgccaaaat agttttcgat
     2221 ttaaaactta ctgacgtgta ttggtgcacc gcggatattg gttggatcac tggtcactct
     2281 tatgtggtgt atggcccgct agcagcagcc ggcaccgttt tcctttacga gggcgcaccg
     2341 acgcacccga cgcctgatcg tttttggaac atgatcgcgc gccaccgcgt ttcgattttt
     2401 tacactgcgc cgacggccat tcgctcgttc atgcgtctgg gaaatgaact tccaaagaaa
     2461 catgatcttt cttcgctgcg cgtattaggg tcggtggggg agccgattaa ccctgaagcg
     2521 tggatgtggt accacgatat aattggtggc ggtcgatgtc cgatcgtcga cacctactgg
     2581 cagacggaaa ctggcgcgat agtgatttcc cctcttcctg gattcattgc aaccaaaccg
     2641 ggctctgcca ctcggccttt gcccggttac gatgtcgacg tagtggataa ggccggaaaa
     2701 tcagttaaaa acggcaccgg cggttatcta gtgatacgca aaccgtggcc gtcgatggca
     2761 cgtacgattc atggagatcc cgaaagattc aagaagcagt atttttctga atttaaaggt
     2821 ggggtttatt ttaccggcga cggtgcaagg cgcgatcgcg acgggtatat ttggtgtctt
     2881 ggtcgagtcg acgatgtttt gaacgtgagt ggccatcggt tgggaacaat ggaagtggaa
     2941 agtgccttag tttcccaccc cgctgttgcg gaagcagcag tcgtaggccg gccagacgaa
     3001 ctcaaggggc agggaatcgt tgcatttgtt actctcaaag cgagtcatgc tgcgcaactt
     3061 cgtggcaatc tatccgaaaa agcccaattt gaaagtctcc tcaagggtca tgtcgttaaa
     3121 cagattgggg cgctcgcacg gcccgacgag attcgtttta ccaaaaattt gcccaagacc
     3181 cgctctggta agattatgcg tcgactactt cgcgagttgg ccaccaacgg tacagttcag
     3241 ggcgacacta ccaccttgga agatttgagt gtgatcgcag ctttgcaaaa ggatgaagag
     3301 tagtttagac caaggcccaa tgaaagctct gcttcgtcta gttgaacgta gtgatccgaa
     3361 tgagtgccaa cgttttgctc agtgggtgaa cacgcactat cctagtcgat tgccgctcga
     3421 agctcgaact gtggcttcac ccgatcattt ttctttttgg gtttttgccg cggattctgg
     3481 tgaaaaaggg gaactgcttg gagccacctc gtttgccgag cgcacgcccc acctggctga
     3541 gactatgcgc acggttgttg
//
LOCUS       DAOMXO010000188         3552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14507_length_3552_cov_3.503860, whole genome shotgun sequence.
ACCESSION   DAOMXO010000188 DAOMXO010000000
VERSION     DAOMXO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14507_length_3552_cov_3.503860"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..998)
                     /locus_tag="PLH57_05055"
     CDS             complement(168..998)
                     /locus_tag="PLH57_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02012.1"
                     /translation="MRVLSVCTAFFCACAHVPDFRPSRPIEKAATWHEALLLCTEYRI
                     EENEKEVDVLKIIPWVRCAEKVFFTHASAKSNEAFALFFRELSIFESRYVAKAQEVVE
                     SVDPRTFNAAVLASFRWIALAAESNSDEPIMFEGDPGVVAQVFPDYAYYLAKRGAFPK
                     KTAISALKPTPRRRPEVEAALAMKKETLAGSVLERSLVYWRKRTSNKVAPNAHAAECL
                     TKLARQANLQTLEELEDYRSSLEPSMSRLRVERRIDELLVELGKNFKSEGFELARCLR
                     "
     gene            1009..1950
                     /locus_tag="PLH57_05060"
     CDS             1009..1950
                     /locus_tag="PLH57_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRK02013.1"
                     /translation="MNTHIPEKSKVAISFPGRSDFRVVPPNEAYVRVFDRSFHFGDSV
                     YEVVRTYDGVLFGMKEHTDRLRNSMKLGLWAHLPDFSLIERMIKDTCRQYFSEFGRKD
                     LYVRVTVSRGIGDTNIDPTTCGQPYAVVIAKEIPPAPDWHATKGVEFATVQRRRNSHE
                     ALPPSMKSGNYLNNVLAIVEAKHNGGSDAVMVNAHGFVTEGTTNNLFIVKNGVVWTPS
                     MDAGILEGITRAMILNLCRQHKIPVEERLFTIQDVFSCEELFLSSTTREIVPITKLDG
                     QAIGNAKPGPVTVSLRKLLREHIASEVKKQPSLFEMV"
     gene            1959..2768
                     /locus_tag="PLH57_05065"
     CDS             1959..2768
                     /locus_tag="PLH57_05065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRK02014.1"
                     /translation="MKRNSALKKHLKRALPIAIEAATEAGAFIEKKYGRYRSLSTKPM
                     AGLVTEADRGAEKIILRTLRKSFPLDHYWGEETGRSEAKWKSPFRWHIDPLDGTTNFV
                     HHVPMFCVSIGLEFENTDSVLGVIYQPMMRELFTATLGGGAYRNNQRIRVSKVATVAD
                     ALLATGFSLKVDQYQKTEMQSLSKLLNQSHGIRRIGSAALDLAYVAAGRFDGFYERGL
                     ANWDVAAGMVLVREAGGAVSNARGERFRLSDDTIVATNEKIQGELLKFIES"
     gene            2861..>3552
                     /locus_tag="PLH57_05070"
     CDS             2861..>3552
                     /locus_tag="PLH57_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02015.1"
                     /translation="MSAVYLLDKFSHEAIVIQLFLISVITMSYFGYLLLKKKKYGAAK
                     KQIPDNVVRAYLMELISHAEGFKNQLFGTNFQIPTGKAPDLTASYAATATAAPANAGD
                     AAAVTAAQGLVAELQKQLAASNAKQEEFAKTISQLSADKATLEAKAAQGGAPAAGGAP
                     SKDALEKIAKLEAKLSEYEVIEDDLANLKKYQQENKQLRSQLAALQGGAPAPEAPTET
                     PAAPVEAIVMPNT"
BASE COUNT          956 a          948 c          816 g          832 t
ORIGIN      
        1 cgattcggtg cttcgtgaga agagtgcgtt caacacactc acctcccatc gtcactaaaa
       61 gtccctcaag ttcgtccaac ccccattcgc tatcgctaac gataagagct cttcgtttat
      121 cgaggggaat taaaaaagca ttcgtatcaa acatagaagc gccactctta ccgcagacaa
      181 cgggcgagtt caaatccctc acttttaaag tttttaccga gttccactag cagttcgtcg
      241 atacgccgtt ccacccgcaa gcgactcatc gatggctcca gggacgaacg atagtcttcg
      301 agctcctcga gagtttgcaa gttggcttgc cgagccaatt tggttaaaca ttcggcggca
      361 tgagcattgg gagccacttt attgctggtg cgttttcgcc aatacactaa acttcgctca
      421 agcaccgaac ccgcaagtgt ttcttttttc atggctagcg cggcttcgac ctcgggcctg
      481 cgtcgcggag tcggttttaa cgcactgatg gcggtctttt ttggaaatgc cccccttttt
      541 gccaagtaat aagcataatc cggaaaaacc tgcgccacca cccccggatc cccttcaaac
      601 atgatcggct catcactgtt cgattctgcc gctaacgcga tccatctgaa actggctagc
      661 actgctgcgt taaaagtgcg tgggtctacc gattccacta cctcttgcgc tttcgcgaca
      721 taccttgatt cgaaaatgct caattcccga aaaaaaagtg cgaatgcttc gttggatttg
      781 gcggaggcat gcgtaaagaa tactttttca gcgcagcgca cccacggaat aattttgaga
      841 acgtcaactt ccttttcatt ttcctcgatg cggtattccg tacacaataa aagtgcttcg
      901 tgccaagtag ccgctttctc gattgggcgg cttggtctaa agtcagggac atgggcgcac
      961 gcacaaaaaa aggcagtgca cactgaaagg actcgcatga acggtttcat gaacacacac
     1021 ataccagaaa aatctaaagt tgcgatctcg ttccctgggc gtagcgactt ccgcgtggtg
     1081 ccgcccaacg aagcctatgt gagggtattc gacagaagct ttcattttgg tgattcagtt
     1141 tacgaagtcg tacgcactta cgacggcgta ctcttcggca tgaaggaaca cacagatcgc
     1201 ttgcgtaatt ctatgaaact tggtttgtgg gcccacctgc ccgacttctc gttgatcgaa
     1261 cgcatgatta aagatacttg tcgccagtat ttttcggagt ttgggcgcaa agatctttac
     1321 gtacgcgtta ccgtgagtcg cggcataggc gacactaaca ttgacccgac aacctgtggc
     1381 caaccttacg ccgtagtgat cgctaaagaa attcctccag ccccagactg gcatgcaacc
     1441 aaaggcgtag agttcgcaac tgttcagcgg cgccggaact cccacgaagc cctcccccct
     1501 tcgatgaaat ccggaaacta tttgaacaac gttctcgcca tagtcgaggc aaaacacaac
     1561 ggcggctccg acgccgtaat ggtcaatgcc cacggttttg tgaccgaagg aacgaccaat
     1621 aatctgttca ttgttaaaaa tggcgtagtt tggacgcctt cgatggatgc gggtattctt
     1681 gaaggcatca cccgggccat gattttgaat ctctgtcgcc aacataaaat tccagtcgaa
     1741 gaacgacttt tcaccataca agatgtattt tcctgtgaag aattatttct atcttcgacg
     1801 actcgggaaa tcgtaccgat caccaaactc gacggtcaag caatagggaa tgcaaaaccg
     1861 ggacctgtca ctgtttcatt gcgcaaactt ctgcgcgaac acatcgctag cgaagttaaa
     1921 aaacaacctt cacttttcga aatggtttag aacttttcat gaaacgaaac tctgctttaa
     1981 aaaaacatct aaaacgagcg ttgccgattg ccattgaagc tgccactgag gcgggcgcat
     2041 ttatagaaaa aaaatatgga cgctatcgct cactgtcaac caaaccgatg gcgggcctgg
     2101 ttacagaagc tgatcgcggt gccgagaaga taattctccg aactctgcgc aagagttttc
     2161 cacttgatca ctactggggt gaggaaaccg gacgcagcga agccaaatgg aaaagcccat
     2221 ttcgttggca cattgatccg ctcgacggaa ccactaactt cgttcaccac gtccctatgt
     2281 tttgcgtttc gatcggacta gagttcgaaa ataccgactc cgtcttgggc gtaatttatc
     2341 agccaatgat gcgagaactg ttcaccgcca ccctgggcgg gggtgcctat cgcaacaatc
     2401 aacgaattcg cgtatcgaaa gtcgccactg tggccgacgc acttttagcc acgggtttta
     2461 gccttaaagt tgatcagtac caaaaaaccg aaatgcaaag tctcagtaaa ctattaaacc
     2521 agtcccatgg cattcgccgc ataggcagcg ccgcactaga tcttgcttac gtcgcagcgg
     2581 gacgattcga cggtttctat gaacgcggct tagccaactg ggacgtcgcc gctggcatgg
     2641 tccttgttcg agaagcaggg ggcgcagttt caaacgctcg aggcgaacga tttcgattga
     2701 gcgacgacac gatcgtggcc accaacgaaa agattcaggg cgaattatta aaattcattg
     2761 aatcttagct ttaccccgtg ccgtcgaagg caaaacctgc cccgcattga cgcgcacgcc
     2821 aagtcacttc agaatggtaa tttaaggggg gcatcaggct atgtctgctg tctatttgct
     2881 cgataaattt tcacacgaag cgattgtcat tcaactcttt ctgatttcag tgatcacgat
     2941 gagttatttt ggttacctac ttctcaaaaa gaaaaaatac ggtgctgcca aaaaacaaat
     3001 tccagacaac gtcgtacgag cctatttaat ggaactcatt tctcacgccg aaggcttcaa
     3061 gaaccagctt ttcggcacca atttccaaat tcctacgggt aaggctccgg atctgacggc
     3121 cagttacgcc gcaactgcaa cagccgcgcc ggcaaacgcc ggtgacgctg ccgccgtaac
     3181 ggccgcgcaa ggtctcgtgg cagaactgca aaagcaactt gctgcctcta atgccaaaca
     3241 agaagaattt gcaaaaacca tcagccaact ttcggccgat aaagcgacgc tcgaagcaaa
     3301 ggccgcccaa ggtggcgctc ccgcagcggg aggggctcct tcgaaagatg ctctcgaaaa
     3361 aatagctaaa ctcgaggcaa aactttcgga atatgaagta atcgaagatg atctagccaa
     3421 tttgaaaaaa taccaacaag aaaacaaaca acttcggtca cagttggccg ctcttcaagg
     3481 aggcgcccca gccccagaag ctccaactga aacacccgca gcgcccgtcg aagctatcgt
     3541 catgccaaac ac
//
LOCUS       DAOMXO010000189         3538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14600_length_3538_cov_5.821418, whole genome shotgun sequence.
ACCESSION   DAOMXO010000189 DAOMXO010000000
VERSION     DAOMXO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14600_length_3538_cov_5.821418"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLH57_05075"
     CDS             complement(<1..1101)
                     /locus_tag="PLH57_05075"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014245578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRK02016.1"
                     /translation="MGEINSGIWEESPLYRNAVLQLNEAGSTMHLDPNVLERLKRPKR
                     ALVVSVPIRLDDGAIAVFEGYRVQHNMTLGPGKGGIRYHQDVNLSEVAGLAMLMTFKC
                     SLVGLPLGGAKGGIRVDPNLLSRQEKQALTRRYTTEINMIIGPDRDIPAPDVGTDAQT
                     MAWMMDTFSQERGYAIPGVVTGKPVEIGGSLGRAESTGRGVVYTIVQAYQKLGMNLGP
                     STTVSVHGFGKVGAVAAEEMEMLGCKVVAVSDVSGGYYNKNGIKIRELTQYLAKNRTM
                     VGFPGAEPITNDELIALKVDIFIPAAIDGVIHGDNVNRVSAKVIAEGANGPITADAHK
                     VLVDKGVLIIPDILCNAGGVIVSYFEWVQGLQN"
     gene            complement(1127..2539)
                     /locus_tag="PLH57_05080"
     CDS             complement(1127..2539)
                     /locus_tag="PLH57_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRK02017.1"
                     /translation="MRRVLVVDDERNITFVIKAILERGGFEVDVFNSSVEALKRLSEP
                     RVPYCAVVTDLYMPDQDGMAVLAHMKDTHPDIPVVMITAFGTVDSAVQALKKGAFDYI
                     TKPFEQGEILAIIEKAANTFQLRNTEPSNESGDSRLSKTLVIGKSPAMQEVVRIVRKV
                     ALSPSTVLITGESGTGKELVALEIHRHSERVHQPFIKINCAAVPHHLIESELFGYEKG
                     AFTGAVNSKPGRFELAHEGTLFLDEVAEMPLEMQAKLLRVIQEAEFERVGGLQTIKIN
                     VRIVAATNKDLAKEVREGRFRDDLYYRLNVVSLLLPPLRERRVDIEDLVYFFIKKFND
                     KLGKRIESVDSVCLKTLCSYSWPGNIRQLENVLERAILMSEGSILRSIDLPEEVFRGE
                     ASLPASSPAAEAFYDGVPFKDAVRKQTQAIEREIIEKSLLENEGNITRTAERLGLSRK
                     GLQLKLKELGLSSQGPRTSL"
     gene            complement(2553..>3538)
                     /locus_tag="PLH57_05085"
     CDS             complement(2553..>3538)
                     /locus_tag="PLH57_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK02018.1"
                     /translation="EIEQGLSHNQREHHQSVLLSLRALGANLAIPFFDEDRVLGFVVA
                     QVEMANQEDDWSLLSVVYGYFAAAARILKNMDVYVRIREKERLAALGQMSAGLAHEIR
                     NPLGAIKGAAQVLEGEKTVQESPFLKVIVEEVNRLNKVVTQFLDYSKPVQSDYTVVDI
                     DSVLQKTAALFQSLRHEGIRIEVHTSGVPGSARVRCSPEQIKQVLVNLIQNSIQSLER
                     SRPAEGGVVRVGVNCDTKAIEPHVNVFVEDNGQGIAREDLDKIFIPFFTKKQKGTGLG
                     LSICSKIAEAHGGRLDVVSDENKNTRFTLRLRAITRENSVKQPLPSLENPI"
BASE COUNT          867 a          964 c          758 g          949 t
ORIGIN      
        1 gttttgaagc ccctgaaccc attcgaagta agaaacaata acgccacccg cattgcagag
       61 aatgtccgga atgatcagca cgcctttatc gaccaaaact ttatgggcat cggcagtgat
      121 cggaccgttg gcgccttcgg caatcacttt tgctgacacg cggtttacgt tgtcaccatg
      181 aattacgccg tcgatagcag ccggaataaa aatatccact tttaatgcaa tcaattcgtc
      241 gttggtaata ggttctgctc ccggaaaacc taccatcgta cgatttttag caagatactg
      301 tgtcaattca cgaatcttga ttccattttt gttgtaatag ccacccgaca catcactcac
      361 ggcgaccact ttacaaccga gcatttccat ttcttctgcc gcaacggctc ccaccttacc
      421 aaaaccgtga accgacacgg tcgtgcttgg accgaggttc attcccaatt tttggtaggc
      481 ttgaacgatg gtgtaaacca cccctcgtcc cgtactctct gctcgcccta ggctgccgcc
      541 gatttctact ggcttaccgg taaccacgcc aggaatcgcg tacccgcgct cttgagaaaa
      601 agtatccatc atccacgcca tggtctgagc gtcggttccg acatctgggg caggaatatc
      661 gcgatctggc ccaatgatca tatttatctc agtcgtataa cgtcgtgtga gcgcctgttt
      721 ttcctgtctc gaaagcaaat tgggatcaac ccgaatgcca cccttcgcgc caccaagtgg
      781 caaacccacg agcgaacact taaacgtcat gagcatcgct agacctgcta cttcggaaag
      841 attcacgtct tgatggtagc gaattccacc tttccctggt ccgagcgtca tgttgtgctg
      901 tactcgataa ccttcgaaaa ccgcgattgc tccgtcatcg aggcggatag gcacgcttac
      961 cacaagagct cgcttaggtc gctttagccg ctccaaaaca ttggggtcaa gatgcatggt
     1021 cgagcctgct tcattgagtt gcagaactgc attcctatac aacgggcttt cttcccaaat
     1081 gcccgaatta atttcgccca tgaacttctc ctctcacttc ctcgatttaa agactagttc
     1141 tcggaccctg cgaagacagt ccgagttcct ttaattttaa ttgcaaacct ttgcgcgaga
     1201 gcccaagtcg ctccgccgta cgagtgatat tgccctcgtt ttctaacaat gatttttcga
     1261 taatttcgcg ttcaatcgcc tgtgtttgtt tgcgaaccgc gtctttaaag ggtaccccgt
     1321 cataaaatgc ctcggccgct gggctcgaag ccggcaaaga tgcttcccca cggaacactt
     1381 cttcaggaag gtcgatagat cgaagtatcg aaccttcgct catgagtatt gcacgctcta
     1441 aaacattttc gagttgccga atgtttccag gccagctgta actgcaaagt gttttcaaac
     1501 aaacggagtc tacactttca attcgctttc caagcttgtc attgaatttt tttataaaga
     1561 aataaaccaa atcttctata tcgactcgac gctcccggag cggcggtaaa agcaatgaaa
     1621 ctacgttcaa acgataatat aagtcatcac gaaagcgccc ttctctcacc tctttggcga
     1681 gatctttatt ggtcgcagca actattcgta catttatttt gatggtttgc agaccaccga
     1741 cacgctcaaa ctccgcctcc tgaatgactc gtagaagctt agcttgcatt tctagtggca
     1801 tctcagccac ttcatctaaa aagagcgtac cctcatgcgc taattcgaaa cgccccggct
     1861 tagagtttac ggcaccggta aaagcccctt tctcatagcc aaataattca ctctcaatga
     1921 gatgatgcgg cacggccgcg cagttgatct ttataaaggg ctgatgcacc cgctcggagt
     1981 gacgatgtat ttccaacgca accagttctt tgccagtccc cgattcaccc gtgatcaata
     2041 cggtcgatgg agacaacgca accttgcgga cgatgcgcac cacttcctgc atcgcgggac
     2101 tttttccgat tactagtgtt tttgatagcc gagagtcgcc gctttcgttg ctgggctcgg
     2161 tatttcgcag ttgaaacgta ttggcagctt tttcgatgat ggcgagaatt tcgccctgtt
     2221 caaagggctt cgtaatataa tcaaacgccc ctttttttaa agcttgcacg gcgctatcga
     2281 cggttccaaa cgcagtgatc ataaccaccg gaatatcggg gtgcgtatct ttcatgtgcg
     2341 ctaaaacggc catgccgtct tgatcgggca tataaaggtc ggtcaccacc gcgcagtacg
     2401 gaactcttgg ttctgaaaga cgcttgagcg cctcaactga agaattgaat acgtcgacct
     2461 caaacccgcc acgttcaaga atcgctttaa tcacgaaggt aatatttcgt tcgtcgtcta
     2521 cgaccaatac tcgtctcatc ggtagggcca tcctatattg gattttctaa cgacggcaag
     2581 ggctgtttca ccgagttttc cctagtaatt gctcgaaggc gcagcgtaaa tcgggtgttc
     2641 ttattttcgt cactcacaac gtctagacgc ccaccatgcg cctcagcaat tttagagcaa
     2701 atcgatagac caagaccggt gcctttctgt tttttggtaa agaaaggaat aaatatttta
     2761 tcaaggtcct ctcttgcgat tccctggcca ttgtcttcaa caaaaacatt cacgtgcggt
     2821 tcgattgcct tggtgtcgca attcacccca acccgtacca cacccccctc agccggacgg
     2881 ctccgctcca acgactgaat gctattttga ataaggttca ctaatacctg tttaatttgt
     2941 tcgggcgaac aacgcactct agccgagcct ggcacaccac tagtgtgaac ctcgatgcga
     3001 atcccctcat ggcgcaggct ctgaaacagc gcggccgttt tttgtaaaac actatcgata
     3061 tccaccacgg tgtagtcgct ttgaactggc ttagagtagt cgagaaattg cgtcaccact
     3121 ttgttcaggc gattcacctc ttcgactatg actttgagga acggcgattc ttgcactgtt
     3181 ttttcaccct caagcacttg agctgctcct ttgattgcac caagagggtt gcgaatttca
     3241 tgtgccaaac cggcagacat ttgcccgagt gccgctagcc gctctttttc tcgaatgcga
     3301 acgtaaacat ccatgttttt taaaattcgt gcggcagctg caaaataacc gtaaacgacc
     3361 gaaagcagac tccagtcgtc ttcttgattt gccatttcga cttgagccac tacgaatccc
     3421 aaaactcggt cctcgtcgaa aaacggaatt gcgagattgg ctccgagagc tcgcagactc
     3481 agcaaaacag attgatggtg ttcgcgttga ttgtgactca acccctgttc gatttcat
//
LOCUS       DAOMXO010000190         3536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14617_length_3536_cov_4.553864, whole genome shotgun sequence.
ACCESSION   DAOMXO010000190 DAOMXO010000000
VERSION     DAOMXO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14617_length_3536_cov_4.553864"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PLH57_05090"
     CDS             <1..679
                     /locus_tag="PLH57_05090"
                     /inference="COORDINATES: protein motif:HMM:NF020752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02019.1"
                     /translation="LKYMHLCFHFRSGVPFVKDTLSQVLGELERVYLAKRWAEKVIRE
                     SAHVQKTANESADHTAAWKALLGRADSAFVGMQDELYADLNTPGALGHLFTLIREINR
                     VEATAAGKPGAEAPLNATRTTVAQEFLNFLERRLVPLWNVFNEPSEAFLKTIDSLRLT
                     VKEVFDGADASAQVDAAEIEKLIAERNAARASKNFARADEIRKLLDAQGIVLVDGAGG
                     TSWKHKT"
     gene            741..2849
                     /gene="uvrB"
                     /locus_tag="PLH57_05095"
     CDS             741..2849
                     /gene="uvrB"
                     /locus_tag="PLH57_05095"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRK02020.1"
                     /translation="MRSASKSMFKLYTDFQPTGDQPGAIDKLVAGIKEGRQHQVLLGV
                     TGSGKTFTAANLISKVNKPTLVLAPNKTLAAQLFSEFKQFFPENAVEYFVSYYDYYQP
                     EAYVPSTDTYIEKDSAINEEIDKLRHSATRSLLDRSDVIIVSSVSCIYGLGSPEAYEG
                     MILYVEEGRKTKREEILNKLVEIQYQRSDIDFHRGTFRVRGDIIDVFPAYEEDLAIRI
                     ELFGDTVEAIFELDPLRGVKSRKLQRVTIYPGSHYVTTKEIRKRAIESIRLELLEHLQ
                     LLNKNGKIVEAKRLEQRTMFDLEMMEELGYCQGVENYSRHLTGRKAGEAPPTLLEYFP
                     KDFLLLIDESHVTVPQIGGMYRGDRARKMTLVEHGFRMPSALDNRPLSFEEFEKAGGS
                     RIYVSATPGPYELNATGGEVVEQIIRPTGLVDPVVEVRPATSQIDDLLVEIKKTVDAG
                     FRVLVTTLTKRLAEELANYYRQAGVKVKYLHSDIETLERVEILRELRQGGFDVLIGIN
                     LLREGLDLPEVALVAILDADKEGFLRSARSLIQTIGRAARNSEGRVIMYGHKTTDSML
                     SAISETERRRKIQIAYSEKHGITPTTVKKAIHARIVETVSDMVKAAEEAGQERKNRLG
                     PASDLMPDYVAEDQRVLRVLDELNIPKKKRNASDIQKMVTELDTKMKAAAKALDFELA
                     ASLRDQMRKLKVVELVLGSG"
     gene            complement(2876..>3536)
                     /locus_tag="PLH57_05100"
     CDS             complement(2876..>3536)
                     /locus_tag="PLH57_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02021.1"
                     /translation="DSRRFICRKMKFLAKIAVVPLLFMVISACSGKKPANKLYSEDLV
                     GKTVAIAQIDAAKESRAHVEVAIINEVIDQGRFEVLDRTTVLDALSTYPDRSDWKRLG
                     DFLKSDFILGVKVVDFTVKERQGYDTIEEDDSLLTEESGSSKTVRGKRHVKVKGNAGK
                     VRLKLTFFDVAQDKVLHEGFGEASETVNSRDNPEGGKMALLERLTAMAVRNFFEALPR
                     D"
BASE COUNT          955 a          846 c          888 g          847 t
ORIGIN      
        1 tttgaaatac atgcaccttt gctttcactt tcgctcgggt gtgccgtttg taaaagacac
       61 gctctcgcaa gtattgggcg aattagagcg agtctatcta gctaaacgct gggcagagaa
      121 ggtgattcgc gaaagtgccc atgtccaaaa aacggcgaac gaatcggcgg atcacaccgc
      181 ggcatggaaa gcgctgctcg gtcgcgccga ctctgcgttt gtcggcatgc aagacgaact
      241 ctacgccgat cttaataccc caggcgcctt gggccatttg ttcacgctca ttcgagaaat
      301 taatagagta gaggccaccg ccgctggaaa acctggagcg gaggcaccgc tgaacgcgac
      361 acgcaccaca gtggcacagg aatttctaaa ttttttagaa aggcgattgg ttccactgtg
      421 gaacgtattt aatgaaccga gcgaagcgtt tttaaaaact atcgattcac ttaggctgac
      481 ggtcaaagaa gtgttcgacg gagccgatgc ttcggcacag gtagatgccg cagagattga
      541 aaaactcatt gccgagcgca acgcagcaag agcgagcaag aacttcgcac gtgccgacga
      601 aatccgaaag ctactcgacg cacaaggaat cgtgctggtc gacggtgccg gcggtacatc
      661 ctggaagcat aaaacgtaga tagttaaact ctccaaccac cgctcttgat ctgaatcgag
      721 agtaacaggt ataagaggct atgcggtctg cgtctaaatc aatgttcaag ctctataccg
      781 attttcaacc taccggtgac caacccggtg cgatcgataa gttggtggcg ggaataaaag
      841 aagggcggca gcaccaggtc ctgcttggtg tgactggatc aggcaaaacc tttacagcag
      901 ccaatcttat atcgaaggtc aataagccga ccctcgtact ggcaccgaat aaaaccttgg
      961 ccgcgcagct attttcggag tttaagcagt tttttccaga aaacgcagtt gagtactttg
     1021 ttagttacta cgactactac cagcctgagg cctacgtgcc atcgaccgac acctacatcg
     1081 aaaaagattc cgctatcaac gaagagatcg acaagctgcg gcactctgct acacgttcgc
     1141 tgctcgatcg tagtgacgtg atcattgttt caagcgtatc ctgtatttat ggtttgggtt
     1201 cgcctgaagc ttacgaagga atgatcctct acgttgaaga gggtcgaaaa accaaacgcg
     1261 aagaaatttt aaataagttg gtagaaattc aatatcaacg cagcgatatc gactttcacc
     1321 gcggcacctt tcgggtgcgg ggtgatatta tcgatgtatt cccggcctac gaagaagatt
     1381 tggccattcg tattgaactt tttggcgaca cagttgaagc aatttttgaa ttggacccat
     1441 tgcggggcgt gaagtctcga aaactgcagc gcgtgaccat ttacccaggt agtcactatg
     1501 taaccaccaa agaaattcga aaacgcgcga ttgaaagcat tcgcctggag ttgctcgaac
     1561 acctacaatt gctcaataaa aacgggaaaa ttgtcgaagc caagcgccta gaacaacgta
     1621 ctatgtttga cctcgagatg atggaagagc tcggttattg ccaaggtgta gaaaactatt
     1681 cgcgccattt gactggccga aaggcaggtg aggcgccacc tacgttgcta gagtattttc
     1741 ccaaagattt tttgctttta atcgacgaga gccatgtaac cgtgccccag atcggtggaa
     1801 tgtatcgcgg cgaccgcgct cgcaaaatga ccctcgttga acatggtttt cgcatgccct
     1861 cagcgctcga caatcggccg cttagttttg aagaatttga aaaagccggt gggagtcgta
     1921 tttatgtttc ggcaacccca ggcccctacg aactaaacgc caccggcggt gaagtggtcg
     1981 aacaaatcat tcgtcccact gggctggttg acccggtggt agaagttagg ccagcgacgt
     2041 cgcaaattga cgatctgcta gttgaaatca aaaaaactgt cgatgcaggg tttcgcgttc
     2101 tggttacgac attaaccaag cgtttggccg aagagcttgc gaattattat cgccaggcag
     2161 gagttaaggt gaagtacctg cattccgaca tcgagacgct tgagcgtgta gagattttgc
     2221 gagaactacg ccaggggggc ttcgacgttt tgatcggtat taatctgttg cgcgaagggc
     2281 tcgatcttcc cgaggtagcg ctggtcgcga tacttgacgc cgacaaagag ggtttcttgc
     2341 gaagtgcccg ctcgctcatt caaacaatag gtcgagcagc ccgaaactcc gaagggcgag
     2401 tgatcatgta tggccacaaa accaccgaca gtatgttgag tgcgatatcc gaaaccgagc
     2461 gccggcgaaa aatccaaatc gcttacagcg aaaaacacgg cattactccg accactgtga
     2521 aaaaagcgat tcacgcccgc atcgtggaaa cggtgtccga catggtgaag gctgcggaag
     2581 aagctggcca agaacgaaag aatcgacttg ggcctgccag tgatttgatg cccgattatg
     2641 tggccgaaga ccagcgagtc ttgcgcgtac ttgacgaatt aaatattccc aagaaaaagc
     2701 gcaatgcttc ggatatccaa aaaatggtga ctgagctaga cactaaaatg aaggctgccg
     2761 ccaaggccct tgatttcgaa ctcgcagctt cgctgcgtga tcaaatgcgc aaactaaaag
     2821 tcgtcgagct agtgttaggg agcggataat aatctctatt taacgaaatt atttctcaat
     2881 cgcggggtag cgcttcaaaa aagttgcgta cagccatagc agtgagccgc tcgagcagcg
     2941 ccatttttcc gccctcggga ttgtcccgtg aattgacggt ttcggaagct tcaccgaagc
     3001 cttcatgcaa caccttgtct tgcgctacgt caaaaaaggt tagttttaaa cgaactttac
     3061 cggcattacc ttttactttt acgtggcgtt taccgcgaac tgttttagag ctgcccgatt
     3121 cttcggtgag tagcgaatcg tcttcttcga ttgtgtcgta accttggcgc tctttaacag
     3181 taaagtcgac taccttaacc cccaaaataa agtctgattt caagaagtcg cctagacgct
     3241 tccaatcgga tcggtcgggg taggttgaaa gtgcatctaa tacagtggtg cgatccaata
     3301 cttcgaaacg accttgatca attacttcat tgataatcgc gacttctaca tgagcgcgcg
     3361 attctttagc ggcgtcaatc tgcgcgatag ctacggtttt acctacaagg tcttcactgt
     3421 ataatttgtt ggctggtttt tttccggaac atgccgaaat caccatgaac aaaagcggta
     3481 cgactgcgat ttttgccaaa aacttcattt tacggcagat gaacctgcgt gaatca
//
LOCUS       DAOMXO010000191         3508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14778_length_3508_cov_6.097596, whole genome shotgun sequence.
ACCESSION   DAOMXO010000191 DAOMXO010000000
VERSION     DAOMXO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14778_length_3508_cov_6.097596"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLH57_05105"
     CDS             <1..596
                     /locus_tag="PLH57_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02022.1"
                     /translation="YFGFFKNAPNIDLANISVHHSHRGRRVSARFLKNSVRFLASISN
                     DRNTSVTLSAVTLGEVRFGAYVWAPFGFEYLAEPGLRPLDVAVRMNQRFVEWLEQSNR
                     EQLFLNPSELTRIKSVARSWRQPWQLATMKTSQIIGRTTGPYDGQLGKAFLGDARQGL
                     ITWNGILFVNRSRSQSVKQFKRHLSATLKLSCWEILE"
     gene            complement(598..>3508)
                     /locus_tag="PLH57_05110"
     CDS             complement(598..>3508)
                     /locus_tag="PLH57_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRK02023.1"
                     /translation="GGGDSGGGGGGGGGGADTTPPTVSSVSASTANGSYKAGDTIDVT
                     VTFSESVTVTGFPTIELETGVTDRTVSYSSGTGGATLTFTYTIQAGDTSSDLDYVATT
                     SLALAGGTIKDAAGNDATLTLAAPGAANSLGNNKALIVDTTAPTVTGVASNTAAGTYG
                     TTTVIDVRVTFSESVTVAGTPTIELETGTTDRTVNFTSGSPGTVLLFEYTVQAGDTSA
                     DLDYKATTSLTAGTSIRDAAGNDATLTLASPGAAGSLGANEAIVISTATATPASLPGF
                     QIAYGDFDRDGDIDVVIPSLTANEVIPYLNNGSGTLSAGTAVAVGAKPVAVIAGDFNR
                     DGKLDIIVSNWDADTIGVLTGVGDGTFNAMAAVAVGDKPAGLAALDINKDGDLDLVVA
                     NYGADNVQLLSNNGSGTMTAGATTATGTGPASVVAGDWDRDGDQDIAVTNWGGGTITV
                     LATAAGALGADETITVGTNPLGLVAADFNSDGMLDLAAANYGDGDVSVSLNGGAAFGA
                     KTDTAVGTNPAGIAAVRWNAGGSLDLVISNSGSDNYSVLSGVGDGTFGAAANTALGST
                     DPSGVAIGDFNRNGEPDVVFMYSTGATATALASGSGTTGDGTFTVSQTLSFAAGSNGP
                     ENINFADMDRDGDLDMVVGEANLDAIYVSLNDGTGTFGALTGTSLNAVGGVQSVGDIV
                     TGDFDNDGDIDVVVSHANGPQYYSYFSNDGSANLTLSSRTNTGRYVKAVSQIPADIDR
                     DGDLDWIGDDSNNGETVVYLNDGTGTFTAASAGVSYAYYGLRDFALADVSRDGIVDLL
                     FLEIANDIIGRGNGTGSGTFGAASTYSTSSGAHNPLGMDLADYDRDGDLDYAAANSAT
                     TNFGVGLNDGSGAFGAVATFTAADALNTPSEIVSGDFDSDGDIDIALSSYGNDYVYIF
                     SNNGSASFTYTSRVAVGDGPLGLAIGDVNKDGKLDWATTNSNTANPSTISIGTGN"
BASE COUNT          805 a          916 c          963 g          824 t
ORIGIN      
        1 tctatttcgg attttttaaa aacgctccga atatcgacct tgcgaatatt tcggttcacc
       61 acagtcaccg cggtcgccgt gtctcggcca gatttttgaa aaattcagtg cgttttttgg
      121 cttcgatttc aaatgaccgc aacacctcag ttaccttgag tgccgttact ttgggggagg
      181 ttcgttttgg tgcctatgtg tgggcaccgt ttgggtttga gtacctcgct gaaccagggc
      241 tgcggccctt ggacgtggcg gttaggatga accagaggtt cgttgaatgg cttgagcagt
      301 cgaatcgcga acagttattt ttgaatccta gtgaactaac tcgaatcaaa tccgtggctc
      361 ggagttggcg ccagccctgg caattggcga ccatgaagac aagtcaaatt attgggcgaa
      421 ctactggacc ctacgacggg cagttgggca aagccttctt gggagacgcc cggcaggggc
      481 ttattacctg gaatggaata ctctttgtaa atcgatcgag atctcaaagt gtaaaacagt
      541 ttaaacggca cctaagtgct accctcaaac tttcgtgttg ggaaattcta gagtaagcta
      601 attgccggta cctatgctga tggtcgacgg attggccgta ttcgaattgg ttgttgccca
      661 atcaagtttg ccgtctttgt tgacatctcc gatcgctagg ccaagaggac catcaccaac
      721 ggcaacgcgg ctcgtatagg taaaagaagc ggagccgtta ttcgagaaga tgtatacgta
      781 atcgtttccg taagacgaaa gcgcgatatc aatgtcgccg tcactatcga aatcaccgga
      841 aacgatttca gacggagtgt ttaatgcatc ggcggcagta aaagtcgcta ccgcaccgaa
      901 tgcaccgctg ccgtcgttca gacccacgcc gaaattagtg gttgcgctat tcgcggctgc
      961 gtaatcaagg tcaccgtcac gatcgtaatc tgcgagatcc atgcccagag ggttgtgggc
     1021 gccactactt gtcgagtagg tcgaagctgc tccgaaagtt cctgagccgg taccattccc
     1081 gcgaccgatg atgtcattgg caatttccag gaataataga tcgacgatgc catcgcggct
     1141 aacgtctgcc aatgcgaagt ctctaaggcc gtaataggcg taactcacac cagcagaggc
     1201 ggccgtaaaa gttccagtcc catcgttgag gtatactacg gtctcaccgt tatttgagtc
     1261 gtcaccgatc cagtcgaggt cgccgtcccg gtcaatgtct gcgggtattt gggataccgc
     1321 ttttacgtaa cggccggtat tggtgcggct agagagcgta agatttgcgg aaccatcgtt
     1381 agaaaagtag ctgtagtact gcgggccgtt ggcgtggctg acaacgacat caatgtcgcc
     1441 atcattatcg aaatctcctg taactatgtc gccaacagat tgcaccccac ccacggcgtt
     1501 taagctcgtt ccagtgagtg cgccgaacgt tccggtgccg tcgttgagtg atacataaat
     1561 tgcgtcgaga ttggcttcgc caactaccat gtcgagatca ccgtcacggt ccatgtccgc
     1621 aaaatttatg ttttctggcc cgttggaacc ggcagcaaac gaaagtgttt gagatactgt
     1681 aaaagttccg tcgccggtag tgcccgaacc actagcaagg gccgtcgcgg tggcgccagt
     1741 gctgtacatg aaaactacgt ctggttcgcc gttgcgatta aaatcgccaa tggcaacgcc
     1801 cgaagggtcg gtgctaccca atgcagtatt tgctgccgca ccaaaggtgc catcaccaac
     1861 accgcttaag acagagtagt tgtcgctacc gctgttagaa atcacgagat caagagaacc
     1921 accggcgttc cagcgaaccg cggcaatacc ggcggggttg gtgccaaccg ccgtgtcggt
     1981 ctttgctcca aacgctgcgc ctccattaag agatacgctc acgtcaccgt cgccataatt
     2041 ggcggcagcg aggtcgagca ttccgtcgga attaaaatca gccgcaacta gcccaagtgg
     2101 gtttgtgcca acagtaatgg tttcgtccgc acccagagcg ccagcagcgg tggctaaaac
     2161 agtgatggtt ccgccgcccc agttggtaac cgcgatgtct tgatcaccgt cgcggtccca
     2221 gtcgccagca acaacacttg ctggtccagt gccggtcgca gtggtagcgc ctgcggtcat
     2281 ggtgccagag ccgttattag aaagtaactg cacgttatcg gcaccatagt tggcgacgac
     2341 taaatcaagg tcaccgtctt tattgatatc cagagccgcg aggccggctg gtttgtcgcc
     2401 gacagcgaca gcggccatag cgttgaaagt accgtcgccc acgccagtga gtacaccgat
     2461 cgtgtcggcg tcccagttgg agacaataat atcgagttta ccgtcgcggt taaaatcacc
     2521 agcaatcacc gcaaccggtt tagctccgac agccactgca gtgccggcac taagggtgcc
     2581 cgaaccattg tttagatacg gaataacttc gttggcagtt aaactcggga tgaccacgtc
     2641 gatgtcaccg tcacgatcaa agtcaccgta ggcgatttgg aatccaggaa gactagcagg
     2701 agtggctgtt gcggtgctaa tcacgattgc ttcgttggca ccgagtgacc cagctgcgcc
     2761 gggcgaagca agagttaaag tggcgtcatt tcctgcggcg tctcgaatac tagtgccggc
     2821 agtcagcgaa gtggttgctt tataatctag gtcggcagag gtgtcgccgg cttgtacggt
     2881 gtattcgaaa agtagaacag ttccaggtga gcccgaggta aaatttactg tgcgatcggt
     2941 ggtacccgtt tccagttcga tcgttggagt tcctgcaacg gttactgatt cactaaaagt
     3001 aactctgaca tcgattacag tagtggtgcc gtaggttccg gccgcggtgt tggaggcgac
     3061 gccagttacc gtgggagcag ttgtgtctac gataagggct ttgttgtttc caagtgagtt
     3121 tgcggccccg ggcgctgcga gcgtgagagt tgcatcgttc cctgcagcgt ctttaatagt
     3181 tccgcccgcc aaggccaacg aagtagtggc aacatagtct aggtcactcg aagtgtcgcc
     3241 cgcctgaatt gtataagtaa aagtgagcgt cgcaccacca gtgcccgaac tgtaggacac
     3301 ggtgcggtcc gtgacaccgg tttcaagttc gatggtcgga aatcccgtta cggtgaccga
     3361 ttcagaaaat gtgaccgtaa catcgatggt gtcgcctgct ttatacgagc cgtttgcggt
     3421 cgatgcactc acgctggata ccgtcggagg ggtcgtgtcg gctccgccac cgccaccgcc
     3481 gccaccaccg ccactatctc cacccccg
//
LOCUS       DAOMXO010000192         3485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14924_length_3485_cov_3.647230, whole genome shotgun sequence.
ACCESSION   DAOMXO010000192 DAOMXO010000000
VERSION     DAOMXO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14924_length_3485_cov_3.647230"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLH57_05115"
     CDS             complement(<1..410)
                     /locus_tag="PLH57_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02024.1"
                     /translation="MIERLMANASLKALSILIAAVLWFVVLGSRNVEIAKEVPIEVVA
                     PADLIVANEVPDKVSFRLAGPKAFLRNVLNRKEDPIRVNLTAAKPGLITYRFFTDNIQ
                     VPLGVKVLSINPTAIVVKLEVNRKKEIPVRLVMKG"
     gene            complement(407..1273)
                     /gene="cdaA"
                     /locus_tag="PLH57_05120"
     CDS             complement(407..1273)
                     /gene="cdaA"
                     /locus_tag="PLH57_05120"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRK02025.1"
                     /translation="MTELLATIGDSVRPIDLLDIAVVSYIVYRALLLIKGTRAVQMLT
                     GLGILGIAFMISIKFELFTLHWILSYFFDSLILLVIVLFQDEIRRALTYMGKNPFFSS
                     SSTAEEIEIIDEISRAAARLARERIGALIVIERETGLKNFIDTGSKIEAKVRSELLYS
                     IFTPTSPIHDGAAIISEGRIAAAGCFLPLSNDPNIEKKLGTRHRAAFGLSEETDAIVI
                     LVSEEAGEAHLVRNGKMTTNLSEEELRGSLMAILELYDGSRIGRIRLRSFQVRTGTRS
                     KGRDEKAGRDSS"
     gene            complement(1316..3226)
                     /gene="ftsH"
                     /locus_tag="PLH57_05125"
     CDS             complement(1316..3226)
                     /gene="ftsH"
                     /locus_tag="PLH57_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRK02026.1"
                     /translation="MKQSHKTVALWIVFILMFAVLFKVVDENSRHRREIKFSDFIRAV
                     KEKKIAEVTFKGDNSIIGSFKEPQPPDGKQYFETIGDTSSGKIFDILDQNGIIPNYER
                     AEKTPFWQQLLISWFPMALLFLVFYIFMRQIQVGGGKAMSFGKSRARMMTENQTRVTF
                     KDVAGVEEAKEELEEIIGFLKDPKKFTRLGGRIPKGVLLMGPPGTGKTLLARAIAGEA
                     GAPFFSISGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRHRGAGLGGG
                     HDEREQTLNQLLVEMDGFESNDGVILIAATNRPDVLDPALLRPGRFDRRVVVNKPDLK
                     GRAQILAVHARKTPMSSDVRLEFIARGTPGFSGADLENLVNEAALIAARKDKKYLEMS
                     DFEEAKDKVLMGVERRSMVISDEEKKNTAYHEAGHTLVGKVLPGMDPIHKVTIIPRGM
                     ALGLTQTLPQEDRLSLSKDKAENSIAFLMGGRIAEEIIFGHKTTGAGNDIERATDLAR
                     RMVCEWGMSDVLGPLTFGKKEEAIFLGREIAQHRDYSEDTARMIDKEVRDIVERNFAR
                     AKKILTDKIDILHNLAKALLEHEVLDGEEIQAIVDGLPYVKSTTGGSKTPPPVADDGG
                     TVPQGSTPPLPA"
BASE COUNT          838 a          859 c          860 g          928 t
ORIGIN      
        1 cctttcatga ccagccgtac ggggatctct tttttacgat taacttcgag ttttactacg
       61 atcgccgttg gattgattga caacactttt acgcccagcg gaacctgaat gttgtcggta
      121 aaaaaacgat aggtaatgag gcccggtttt gcggcagtta aattgacgcg aatagggtct
      181 tctttgcggt taagaacatt tctcagaaat gccttggggc cggcgagtcg gaacgaaacc
      241 ttgtctggaa cctcgttggc aacaatgaga tcggctggcg ccacgacttc aattggaact
      301 tcttttgcaa tttctacgtt gcgggagccc agcaccacga accaaagaac cgctgcgatt
      361 aaaatgctaa gtgctttgag cgaagcgtta gccatgaggc gttcgatcat gacgaatccc
      421 ttcccgcttt ttcgtcgcgc cccttagagc gagtcccagt gcgaacttgg aaggaccgca
      481 aacgtatgcg accaattcgg gagccgtcgt ataattcaag tattgccatg agacttccgc
      541 gaagttcttc ttctgaaaga ttagtggtca ttttaccatt tcgaaccaaa tgcgcttcgc
      601 cggcttcttc cgaaacaaga atgacgatag catcggtctc ttcggatagt ccgaacgccg
      661 ctcggtgtcg cgttcccagt ttcttttcaa tattggggtc gtttgagagt ggcaggaaac
      721 aaccagccgc agcgatgcgc ccctcggaaa tgattgcggc gccgtcgtgt atcggactcg
      781 tcggagtaaa aatgctataa agaagctcag accgaacttt ggcttcgatc ttagaaccgg
      841 tatcaataaa attctttaat ccggtttcgc gctctatgac gataagggca ccgatacgtt
      901 cacgtgccaa gcgagccgct gctcttgaga tttcgtcgat gatttcgatt tcttctgctg
      961 tgctcgagga ggaaaaaaat gggtttttac ccatataggt gagcgctcgg cgaatttcgt
     1021 cttggaatag aacgattacc aataaaatga gcgagtcgaa gaagtaggaa agaatccagt
     1081 gcaatgtgaa caactcgaac tttatcgaga tcatgaacgc aatgccgagg atccctaagc
     1141 ccgtaagcat ttgtacggcg cgcgtgcctt tgatcagcaa taaagcgcgg taaacgatat
     1201 aagacacgac ggcaatgtcg agtaagtcga tggggcgaac ggagtcgccg atcgttgcta
     1261 aaagctccgt catgaaaacg ttcctcgagg ggcggagagc gttagttaca tcaaactatg
     1321 ctggaagcgg aggtgtgctg ccctgaggaa cagtgccccc gtcgtcagcc accggaggag
     1381 gcgttttcga tcctcctgta gtgcttttta cgtaaggcaa cccgtcgacg atcgcttgaa
     1441 tttcttcacc gtccaacact tcatgctcaa gcaaagcttt agctagattg tgaaggatat
     1501 cgatcttatc cgtcaatatt ttcttggctc tagcaaagtt gcgctcgacg atatcgcgga
     1561 cctctttgtc aatcatgcga gcggtatctt cactgtagtc acggtgctgc gcgatttccc
     1621 ggccaaggaa aatggcttct tcttttttgc caaaagttag aggtccgaga acatcgctca
     1681 ttccccactc gcaaaccatt cgccgtgcca aatctgtagc acgttcgata tcgttgccag
     1741 cgccggtggt tttgtgacca aaaataattt cttcggcaat tcgcccgccc atcaagaatg
     1801 cgatcgagtt ttcggctttg tcttttgaaa gactcaggcg gtcttcttgc ggaagggttt
     1861 gcgttaagcc gagcgccatg ccgcgtggga taatggttac tttatgaata ggatccattc
     1921 ccgggagcac tttgccgacc aaagtgtggc ctgcttcgtg gtatgcagtg ttctttttct
     1981 cttcgtcaga gatcaccatg ctacgacgtt caacgcccat gagcacttta tctttggctt
     2041 cttcgaagtc cgacatttcg agatattttt tgtctttccg ggcggctatg agcgcagctt
     2101 cattgaccaa gttttcgaga tcagcacctg aaaaaccagg agtcccacga gcaatgaatt
     2161 caaggcgcac gtcagaggac attggtgtct tgcgcgcatg aacagccaaa atctgagctc
     2221 gtcctttaag gtctggctta ttgacgacga ctcgtcggtc gaatcggccc ggtcttaaca
     2281 gggccgggtc cagcacgtct ggtcggttgg tggcagcaat taagatcacc ccgtcgtttg
     2341 attcgaaacc gtccatctcg acgagaagct ggtttagggt ttgctctcgc tcgtcgtggc
     2401 cgccaccaag gccggcacca cgatgtcgac ccactgcgtc gatttcatcg ataaatatga
     2461 tgcacggtgc gtgtttctta ccctgttcga aaagatcccg gacgcgagac gcgccaacac
     2521 ctacgaacat ttccacgaaa tccgaacctg aaatcgagaa aaacggagct ccagcttcgc
     2581 ccgcaatagc gcgtgctaaa agggttttcc cggtgcctgg agggcccatc agaagcacgc
     2641 cttttggaat acgaccacca agacgagtga atttttttgg gtctttcaga aaaccgataa
     2701 tctcttcaag ttcttcttta gcttcttcga cccctgcgac atctttgaaa gttacgcgtg
     2761 tttgattttc ggtcatcatg cgagcgcgcg attttccaaa tgacattgcc ttgccgccgc
     2821 ccacctgaat ctggcgcata aaaatataaa aaaccaaaaa cagaagcgcc atcggaaacc
     2881 aggaaattaa cagctgctgc cagaacgggg ttttttcggc gcgctcgtaa ttggggatga
     2941 tcccgttttg atctaggata tcaaaaatct tgccgctaga ggtgtcgccg atggtttcga
     3001 aatattgttt tccatctggt ggctgtggtt ctttgaagga accgataatc gagttatcgc
     3061 ccttaaaagt gacctccgcg attttctttt ctttcactgc tcgaatgaag tcgctgaatt
     3121 taatctcgcg gcgatgacgg gagttttcgt ccaccacctt gaacaggacc gcgaacatga
     3181 ggataaaaac gatccataag gcgactgtct tatgagattg tttcacttct gaccttcttt
     3241 ctgtttcgag ctttgctcga ggaattagct gggttcaaag agggttccca gcggattcga
     3301 ggaccttcaa aaacgacccg gcccccacca ggaaactgta caagttttcg ggggcctccg
     3361 ccactttttg ttgtttttcg ttcctgtaga attcgttcaa gtgtactcca ttggcctcga
     3421 gtagtttttt ggctatttgc gccaagatac cgatttaaaa tggattttaa ttcgtaaaag
     3481 tcaag
//
LOCUS       DAOMXO010000193         3483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14936_length_3483_cov_4.699825, whole genome shotgun sequence.
ACCESSION   DAOMXO010000193 DAOMXO010000000
VERSION     DAOMXO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14936_length_3483_cov_4.699825"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1166
                     /gene="gspC"
                     /locus_tag="PLH57_05130"
     CDS             120..1166
                     /gene="gspC"
                     /locus_tag="PLH57_05130"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HRK02027.1"
                     /translation="MTPSDSSAPRKVAFNEFGDRMDPGTKLILKARTKARVLADRIAA
                     IDWRETLERTRTMVSGRAYGTYFRMGAITLAAYFLADTVSLFTDSFVPDPPVVPPARM
                     SAKSGARKSIEEYGSITSRNIFNSQGLIPDDGVLGQGPARKSNLPLHLVGTVVMKDEL
                     KSIAAIEDKTQSLVFPVRVEDGIDGRIRITKIEHLRVYFINESNGQLEYIEIVDDLGI
                     PALRTSAAAPTKKTGDDEGITNRGETQFDIDRTVVDKALTNINEILTQARAIPNFENG
                     IPNGYKLIQIVPGSIYEKLGLKNGDVITRINGEPITDPAKPIQMFSELRSMNRLELTV
                     KRGGRTQSYGYDIR"
     gene            1180..>3483
                     /gene="gspD"
                     /locus_tag="PLH57_05135"
     CDS             1180..>3483
                     /gene="gspD"
                     /locus_tag="PLH57_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HRK02028.1"
                     /translation="MALKKLVSVGLLIFALGLFVNRTEAQSDAQDGGEAARRGFNAPG
                     SGFGSNPFSPKAVAPTADEEDEEDIEEDEELDELSEEILKSRMESRLRERSQQGGGAA
                     PTPEARVDSRPTSRGSVNFRNASDGIGATSLSGGANAKIDVGNKAGCLKLDPHTGYGP
                     DIVTNFDFPDADIVEIAKTLGRLTCLNFIYDKDVKGRISVVSNAPITIGEAWKAFLTA
                     LEMSNYSIVPSGAYLRITRKREAREKQIKTYSGSFSPDTDTLITRVLPLKYISAEEAA
                     RVFRNFMSPDTRIVNYDQTNTLIITDTGANVKKLVDIIRLLDVEGYDESLEVIRVKNA
                     SAQEIAKLIDQLLPGNQPGGANPPPGVPRFRAGFTARKTKEGGVISHIIPDDRTNSVI
                     VSANGKGHDQVRMLIQKLDSKVAASAGSSRIHVVYLQFADAEQVAQTLNNLASGSGGA
                     KPPGQLGATTTAQLFEGAIKVSADKPTNSIVITGSPSDFQTVSRVIAKLDVPRDQVYV
                     ETIIMEITLDKRFDLGTAAALPNSAISFVPNTDFSSAFGNLLTNPLANTGLVMGFQAG
                     EGTRTFTVPGTNNSITVKSLQGLIMALQQNTVGNVLATPQILTLDNQEAEIEIGETIP
                     VPTVTAVQGAGSQQSFTREKVTLSLKIKPQINKISNFVKLDLKQKYEDFSSRAVQAPV
                     PGTVTRSSSTTVVVQDKDTVALGGLMRDKSEEKTIKVPLLGDIPLVGWLFRSKSTQST
                     KTNLVVFITPHIVKQYQTVRKILDKTLK"
BASE COUNT          974 a          803 c          863 g          843 t
ORIGIN      
        1 ggcaagggct ttgctttgtc actctataga tgaaaatctg ggaccggatt agaaaactaa
       61 gattctcgga tttacgcttt tggaaacgtc ggaagactga tccaaatgcg gaagattcgg
      121 tgacaccatc agactccagc gcgccccgca aagtggcctt taatgagttt ggtgatcgaa
      181 tggacccggg cactaaacta atattaaagg ctcggaccaa ggcacgagtt ctggccgatc
      241 ggattgcggc gatcgattgg cgcgaaaccc tcgaacgaac ccgcacaatg gtctctggtc
      301 gagcatacgg tacctatttt cgaatgggcg ctataacgtt agctgcgtat ttcctggccg
      361 atacggtttc tttgtttacc gattcttttg tgccagatcc cccggttgtt cccccggcgc
      421 gcatgagcgc taaatctggt gcaagaaagt ccatcgaaga atatggctct attacttccc
      481 gcaatatttt taattcccag ggattaatcc ccgacgatgg ggtgttgggc caaggaccag
      541 cgcgaaagag caacttgccg ctgcacttgg tcgggactgt cgtcatgaaa gacgaattaa
      601 agtcgatcgc ggccatcgaa gacaaaactc agagtcttgt gtttcccgtg cgagtggaag
      661 acggcataga cggacgcatt cgtattacca aaatcgaaca tttgcgggtt tattttataa
      721 acgagagtaa tgggcagctg gaatacattg aaatagtcga cgatctcgga attcctgcgc
      781 tacgcacaag tgctgctgct ccaaccaaaa aaaccgggga cgatgaagga atcaccaatc
      841 gtggagaaac ccagttcgac atagatcgga ccgtggtcga taaggccctg accaacataa
      901 atgaaatttt gactcaggcg cgggcaattc cgaacttcga aaacgggatt ccgaacggat
      961 acaaactcat tcaaattgtg cctggcagta tttatgaaaa acttggttta aaaaacggcg
     1021 acgtaattac gcggatcaat ggcgagccaa ttacagatcc ggcaaagcca attcaaatgt
     1081 ttagcgaatt aagaagtatg aaccggttag aactcaccgt taagcgcggt ggccgtaccc
     1141 agagttacgg ttatgacatt cggtgagcga ggaggagtta tggcattgaa aaaactagta
     1201 agcgtggggc tattgatctt cgccttaggc ctatttgtaa atcgaaccga agctcaaagc
     1261 gacgcccaag atggtggtga agcggctcgg cgcgggttca atgcccctgg ctctgggttt
     1321 ggttccaacc cctttagtcc caaagcggtg gctcccactg cagatgaaga ggacgaagaa
     1381 gatatcgaag aagacgaaga acttgatgag ttgagtgaag aaattctaaa gagtcgcatg
     1441 gaaagtcggt tgcgtgaacg ctcgcagcaa ggcggaggtg cagctccgac ccccgaggct
     1501 cgcgtagatt ctcggcctac ctcacgaggc agcgtcaatt tccgaaatgc gagcgacggc
     1561 attggtgcaa cctcgcttag cggtggtgcc aatgcgaaaa tagatgttgg gaacaaagca
     1621 gggtgcttaa agcttgatcc tcataccgga tacggaccag atattgttac caactttgat
     1681 tttcccgatg ccgatatcgt ggaaatcgct aaaactcttg gtcgcttaac ttgtttaaat
     1741 ttcatttatg acaaagacgt gaagggccgc atttccgtag taagtaatgc accgattacc
     1801 ataggcgaag cttggaaagc gtttttgacg gcattagaaa tgagtaacta ttcgatcgtt
     1861 ccgtctggcg cctatttgcg aattactcgt aagcgagagg cgcgcgaaaa acaaattaag
     1921 acctattccg gcagctttag tcccgatacc gatacactca ttaccagagt gcttccgcta
     1981 aaatacatta gcgccgaaga agctgctcga gtgtttcgga attttatgtc gcctgataca
     2041 cgcatcgtaa actacgatca aactaacacg ttgatcatca ccgacactgg cgccaatgta
     2101 aaaaaattgg tcgatataat tagactatta gacgttgaag ggtatgacga gagtttagaa
     2161 gttatccgcg tgaaaaacgc ctctgcccag gaaattgcta aattgattga ccaactttta
     2221 cctggcaatc aacctggagg cgcaaatccg ccgcccggcg tgcctcgttt tcgcgctgga
     2281 tttaccgccc gtaaaactaa agagggtggc gtaatctccc acattattcc cgatgatcgc
     2341 accaactcgg ttatcgtgtc tgccaatggc aagggtcacg atcaggttcg aatgctaatt
     2401 caaaaactag attccaaagt tgcagccagt gcgggttcta gccgtataca tgtggtttat
     2461 ttgcaattcg cggatgcgga acaagtggcg cagactttga acaatctagc ttcgggatca
     2521 ggcggcgcca aaccgccggg ccagctcggt gccactacaa cggcgcaatt gttcgaaggc
     2581 gccataaaag tgtcagcaga taaaccaacg aactcgatcg ttataactgg atcaccaagc
     2641 gattttcaaa cggtgagccg tgtaattgcg aaactcgatg ttcctcgaga ccaagtttat
     2701 gttgagacaa ttatcatgga aatcacgcta gataagcggt tcgatttagg aacggcggca
     2761 gcgttgccga actcggccat ttcgtttgtg ccaaacactg atttcagttc cgcttttgga
     2821 aatttattaa ctaatccttt ggcgaacacc ggtttggtta tgggatttca ggccggcgaa
     2881 ggaacacgaa cattcactgt tccaggtacc aataactcaa ttaccgtgaa gagtttgcag
     2941 ggcctgatca tggcgctaca acagaataca gtgggcaatg ttttagccac tccccaaatt
     3001 ctcactcttg ataaccaaga agcggaaatt gaaattggtg aaacaattcc agtgcccact
     3061 gttacggcgg ttcagggcgc gggctctcag caaagcttta ctcgtgagaa ggttactctt
     3121 tcacttaaga tcaaaccgca gatcaataaa atctctaact tcgtgaagct tgatctgaaa
     3181 caaaaatacg aagacttttc gagtcgtgca gtacaagcgc ccgtaccagg gactgttact
     3241 cgttcttctt caacgacagt cgtggttcaa gacaaagaca ccgtcgcgtt gggtggttta
     3301 atgcgcgata agtccgaaga aaaaaccatc aaggtacccc tgttgggaga tattccgctg
     3361 gtgggttggc tgtttcgttc caaaagtact caaagcacta aaaccaactt agtggttttc
     3421 attacgcctc atatcgttaa gcagtaccaa acagttcgga aaattttgga taaaacactt
     3481 aag
//
LOCUS       DAOMXO010000194         3479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14964_length_3479_cov_5.415012, whole genome shotgun sequence.
ACCESSION   DAOMXO010000194 DAOMXO010000000
VERSION     DAOMXO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14964_length_3479_cov_5.415012"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..2852
                     /locus_tag="PLH57_05140"
     CDS             243..2852
                     /locus_tag="PLH57_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02029.1"
                     /translation="MPALGYFGAHLDSYLIVRSQEFNKIVNSEQIKFTWSRFQLKEID
                     FTNSEAGPLNVRVICVSPKHTKKLGMDSPFLFVGEKSRIEIIQFVYSLYNIHYFLLTA
                     LLIFGLGVVLLLASQKRVNYSHVIISTVLAASTLFSWSTVFDGLLMTLGVPPEIIRVY
                     ALMLWGPTVLYYAIPRVVLRSVLSILWVSLLFIGVATPYGIESQFIYGTYRWMYLSVG
                     VISFLSAIAIRENRYILPYCVLMAFDSLALFGVYSVKAGFYASGFGIFGLYLGANLQS
                     ALELIVHHWYWHQRQELSQSLDLLRSSQSTDSKHWKSRLGEYCRLLARHSGAQKVSLC
                     YLALESPVIITFNRGTIRGIEDGLLPPVFARVIQTGEGLWWVSSEQLATYAPQGTAVR
                     RDVYKSSIAVIVPIKVGSDCYGAISLTEFALKESTSKSSEARITIEQTINSFLDLIAT
                     QVVRQKEQSNNEIQKASTAVVKCLNEKYAIAVSKSEVIEETLKSIANRFGVASMFFEF
                     DAVTERLHLLGSANMDKDLANSWAVLPFRARATNKLSPFAITINEKRNIFIEAITSLF
                     GILAAPSVDVLQDSRTTAFASLHVGIGEKTLGLIALLDPPNQTNLNATIVASLDLPLK
                     YFAFALEQWNSSALITQQEKALRRFASPELIDLIVKGDENESIFGAVRECVIIVLDLR
                     GSTQASRSETDSIVLARKFSEIYQVASDVADEYAATFDKGNGDGMMFTLPVDDHADRN
                     GLRLGVRLLCAVEEKAKRLLPITGSVVVIHRGKPFRGIIGHTHRIAWDTCGKDINDTY
                     AIEKYAKTIPGVVLAVSLPAIEGEAINIKRIFDECSGAPVSVPELPFDIFLFDREKLL
                     AFSQKLLDERVAA"
     gene            complement(3172..3297)
                     /locus_tag="PLH57_05145"
     CDS             complement(3172..3297)
                     /locus_tag="PLH57_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02030.1"
                     /translation="MKNLFAIIALVVAAFGATYAITASVDANSAVVHADDEGGPR"
BASE COUNT          959 a          679 c          809 g         1032 t
ORIGIN      
        1 ccaatcgggg gtgtcttagt atgttacata gttcgagact aaggttagtt ttcgtcatta
       61 ttttggtggt tgtaccagcc gttgttggcc ttactcgaga cctcgtacta tttaagttag
      121 cacgtgttca gaatattaat atagttgaac cggaaagtgt tggaccgatt agttgctcgg
      181 atgaaatgtg tacgcgggaa tatgagtttc tcgtgcgacc taccccgaat tcgcaggtca
      241 cagtgcctgc gcttggttat tttggggccc acctcgactc ctatctcata gttagaagtc
      301 aagaattcaa taagatcgta aactctgaac aaattaaatt tacttggtcg cgttttcagc
      361 taaaagagat tgatttcaca aattcggagg caggaccttt aaatgttaga gtgatctgcg
      421 tttcgccaaa acatacaaaa aaactgggaa tggattctcc atttttgttt gtgggggaaa
      481 agtcacgaat tgaaattata cagtttgttt attcacttta taatattcac tactttttac
      541 ttactgccct actaattttt ggactaggtg tggtgcttct tttagcatct caaaaacgag
      601 ttaattactc acacgttatt atttctacag tgttagcagc ttctactctc ttttcctgga
      661 gtacggtgtt tgatggttta ttgatgacat taggtgtacc tcctgaaatc attcgagttt
      721 atgcattgat gctttggggg cccacggtcc tatactacgc gatacccagg gtagttttga
      781 gaagtgtttt atctattttg tgggtgagtt tactcttcat tggggttgct acgccatatg
      841 gtatcgaatc tcagtttatt tacggcacat atcggtggat gtatctttcg gtgggagtaa
      901 tttcctttct aagcgcaatt gcaattcggg aaaatcgata tattctgccc tattgtgtgt
      961 tgatggcgtt cgacagtttg gccctctttg gggtttattc agtaaaagcg ggtttttacg
     1021 cgtcaggatt tggaattttc ggactttatt taggcgctaa tctgcaatct gcattggaat
     1081 taattgtgca tcattggtac tggcatcagc gtcaggagct ttcgcaaagt ttggacttac
     1141 ttcgaagtag tcagtctacc gattcaaagc actggaaaag tagattgggg gagtactgtc
     1201 gcttgttggc gcgacactcg ggtgcgcaga aagtttctct ttgttactta gcactggagt
     1261 ctccagttat tattactttt aatcgtggaa caataagagg tattgaagac ggtttgttac
     1321 cgccagtatt tgcccgtgtt attcaaactg gcgagggact ctggtgggtc agtagcgagc
     1381 agctggctac ctatgcgccg cagggtactg ccgtgcgacg tgatgtctac aagagtagta
     1441 ttgcagtgat agttcctatt aaggttggga gtgattgcta cggtgcgatc tctctaacgg
     1501 aatttgcact taaagaaagc acatcgaaat cgagtgaagc cagaataact attgaacaaa
     1561 caataaactc atttctcgat ctgattgcaa cgcaagttgt gcgtcaaaag gaacagtcga
     1621 ataatgagat tcaaaaggca agtactgcgg tagtcaaatg tctaaatgaa aaatacgcaa
     1681 tagccgtaag taaatctgag gttattgaag aaacattaaa atcaatagcg aatagatttg
     1741 gcgtggccag tatgtttttc gaatttgatg ctgttacgga gcgtcttcat cttctgggaa
     1801 gtgccaatat ggataaggat ctagcaaatt cgtgggccgt gcttccgttt cgtgctaggg
     1861 caacaaataa gcttagcccc tttgcaatta ctatcaacga aaaacgaaat atttttattg
     1921 aagccatcac ttcattgttt ggaatacttg ctgccccctc cgtagatgtg ctgcaggact
     1981 ctcgtaccac ggcctttgca tccctgcatg tcggaatagg agaaaaaacg cttggtctca
     2041 tcgcgttgct cgatccgcca aaccaaacaa acttaaatgc aacaatagtt gcaagtttag
     2101 acttgccctt aaagtatttt gcttttgcac tggagcaatg gaactccagt gctttaatca
     2161 cccaacagga aaaggcgcta cggcgattcg caagtcctga attaattgat cttattgtta
     2221 aaggagatga aaatgaatct atctttggcg ccgtacgcga gtgcgtaata atcgtgcttg
     2281 atctcagagg atcaactcag gcgtcaagat cggaaacaga ttccattgtt ctcgcccgta
     2341 aattttctga aatttatcag gtagcatctg atgtagctga tgaatacgcc gcgacttttg
     2401 ataaggggaa cggtgacgga atgatgttca ccttaccggt cgatgatcat gcagatagaa
     2461 acggactgag actgggtgtt cgcttgctct gtgcggtaga agaaaaagcg aagagattgc
     2521 tacctattac gggttcggtt gtcgtaattc accggggaaa accgtttaga gggattattg
     2581 gtcataccca ccgtattgcc tgggatactt gtggcaaaga cataaatgac acttatgcga
     2641 ttgaaaaata tgcaaagacg atccctggag tagttctcgc tgtatcttta ccggcaatag
     2701 agggagaggc cataaacata aagagaatat ttgatgagtg ttccggagcc cctgtgagtg
     2761 tcccagagtt gccatttgac atatttctat ttgatagaga aaaactttta gcgttttcac
     2821 aaaagctgct agacgagcgt gtcgcagcat gagcgagggc gcgatcatgc cagctgccaa
     2881 aagttccaaa ttcaatgtaa tttaattaag ttagtgactg acctgattaa atattaaaat
     2941 catacctaag ggggcagcag tgtttgtccc tttagctctg caaactgaac atattccaat
     3001 taatactact ttaactactt aaaagcactg tgaaggggca ctaccacgat cggtctaaac
     3061 gtattggtag gcgctcgaaa tacagaatta tttatcagga tgtattgggt ttagatggag
     3121 atcaagaata cggtaaatcg cttgttgatg caggacgtta acaactagaa actagcgggg
     3181 cccgccttca tcatcagcat gaactactgc ggaattagca tctacagaag cagtgattgc
     3241 gtaagtagca ccgaaagcag caacgacaag agcgataatc gcgaataagt ttttcataaa
     3301 tcctccaaat accaagatca gttgcagcta cttattgcat agctgatgct gtgagtcaac
     3361 tgttgcatcc aggcacgtct gcgggtccgt taaaaacttc ggttttaagt ctaaattcag
     3421 agacttccgc ctgccggccg gccaaatgga tcgaccattc actcctctat accaaagac
//
LOCUS       DAOMXO010000195         3479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14965_length_3479_cov_4.674942, whole genome shotgun sequence.
ACCESSION   DAOMXO010000195 DAOMXO010000000
VERSION     DAOMXO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14965_length_3479_cov_4.674942"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /gene="rplT"
                     /locus_tag="PLH57_05150"
     CDS             complement(<1..264)
                     /gene="rplT"
                     /locus_tag="PLH57_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRK02031.1"
                     /translation="MARAKRGFKARRRRKKILNMAEGFTHRRKNTIRRGAEAVDRAKR
                     YAFRDRRARKRDFRQLWITRIGAAALESGVNYSGLIAGLKTSNI"
     gene            complement(292..495)
                     /gene="rpmI"
                     /locus_tag="PLH57_05155"
     CDS             complement(292..495)
                     /gene="rpmI"
                     /locus_tag="PLH57_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRK02032.1"
                     /translation="MPKVKTKRAAAKRFKMTASGKIKFKRAGKRHQLGHRKSAKRKLR
                     LRKGGYVHPSYMDRVKGTLRYTA"
     gene            complement(522..1049)
                     /gene="infC"
                     /locus_tag="PLH57_05160"
     CDS             complement(522..1049)
                     /gene="infC"
                     /locus_tag="PLH57_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRK02033.1"
                     /translation="MVKPQGKDDIRVNERIRVPQVRVIDEEGKMLGVFMTRDAIHMAK
                     DRGFDLLEVSPTAVPPVCKFADYGKYKYEKKKKDHEARKKQAVISVKEVQLRPQTEEH
                     DLDYKFRNVQRFLQEGDKAKVSVMFRGREIAYVEHGHKLLGRLIEMVKDCGIVESPPK
                     LEGKRLTVVLAPLHK"
     gene            complement(1190..1741)
                     /locus_tag="PLH57_05165"
     CDS             complement(1190..1741)
                     /locus_tag="PLH57_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02034.1"
                     /translation="MAFSMAASILAFGCAPQKTIYATLQKCESEYPKANRSTRDLFRA
                     CREGVQIAAEQSVAGIDLQLVEISERNRAGEVSIRTVTGFSSEGLDAYYEQVSQLCFE
                     RYSERERTDACRFGAIKYASEVREYLIKNRFIRCVVLDIRREVGVPRDVLQGRARLTP
                     RDPGEFCILQKDLSTSPHAPESR"
     gene            2064..3449
                     /locus_tag="PLH57_05170"
     CDS             2064..3449
                     /locus_tag="PLH57_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRK02035.1"
                     /translation="MQNERRILVVDSDRDFTQNLVRQNKSSRIDLVIAHNRADALTAI
                     AHARGPFASVLVNKKLADYGTYSVVRQVHVRHLGTPVYLLSEEPIENEDLKENLNALG
                     IRQVLPKPLTYEELVKQLESVLVGFKSVAPSQGIKDSSMVGTELGAPENDFTPIRAQH
                     FLSGHRSHFSVYVQIRSNRFLKILNAGDVFDARRLEQYLARGIQWFHIRKDAHRSYLE
                     YCDFVSKKLIEHGMHSIETQRDQVLNLGEETLRYFGHAGVSGDLIHYAVNFVQDCNQL
                     TKKLQLDRHREIGAFMNNLVEWEHSVSVALVTSLLMRGLRIENERVQNSIGLAALLHD
                     IGKIQTCPSLIHEDLTRMTEQEIATFKDHAWAGATMIAGVPGIDSTTVQAVAQHHERR
                     NGKGFPLGLGAGKINRVAEIVGLADEYTNLIHKNKVLPEITKIHFLQDTLLDGFSTEV
                     VDSLCVLFLRN"
BASE COUNT          888 a          909 c          797 g          885 t
ORIGIN      
        1 aatgttcgaa gttttcaaac ctgcaatcaa gccactgtag ttcactccgc tttctagagc
       61 agctgcaccg atgcgagtga tccagagttg gcgaaaatca cgtttacggg cgcgacgatc
      121 gcgaaacgca taacgttttg ctcgatcaac agcttccgct ccacgacgga ttgtattttt
      181 tctccgatgg gtaaaaccct cggccatgtt caaaatcttt tttcttctgc gacgggcttt
      241 aaatccccgt tttgctcttg ccatttgaat gtactcctat ttcaaaaatt cttaggcggt
      301 ataccgcaac gtgcctttta cgcgatccat gtaggatggg tgcacatagc cgcccttacg
      361 gagacgcaat ttgcgctttg cactcttacg atgacccaat tggtggcgct tgcccgctct
      421 tttaaattta attttcccac tcgcggtcat cttgaatcgt tttgcagccg cgcgttttgt
      481 tttcaccttt ggcataaaat ctccaatctc actaaaatct tctacttatg cagcggagcc
      541 agaaccaccg tcaaacgctt gccttcaagc tttggcggcg actcaacgat tccacaatcc
      601 ttaaccattt caatcagacg acccagcaac ttgtgaccat gctcgacata cgcgatctcg
      661 cgcccacgaa acatgaccga caccttggct ttatcgcctt cttgcagaaa tctctgcacg
      721 ttcctaaact tgtaatcgag gtcgtgttct tcagtttgcg gccgaagctg cacttccttc
      781 acgctgatca ctgcctgctt cttgcgggcc tcatgatctt tctttttctt ttcgtatttg
      841 tacttgccgt aatcggcaaa cttacaaacc ggaggaaccg ctgttggaga gacttccagg
      901 agatcaaacc ccctgtcttt cgccatgtga atggcgtctc tcgtcatgaa tacaccgagc
      961 atcttgcctt cttcgtcgat tacgcgaacc tggggcacgc gaattctttc gttgactcga
     1021 atgtcgtctt taccttgggg ctttaccaat ggagcagcga tcttagattc ctccgttaaa
     1081 aataaaagat gttctcgcct tcatttcaaa ctagacacga aatgaagagt agacgcatcc
     1141 cgttgtctta caaaaagaga ggtggaatta ccatacgatt ttaatgagtt caacggcttt
     1201 ccggggcatg gggcgaagta ctgaggtctt tctgcagaat acagaactca ccggggtccc
     1261 ggggcgtcag tcgagcccgc ccttgaagaa cgtctctggg taccccgact tcgcgacgaa
     1321 tatcgagcac aacacaccga ataaaccggt tttttataag atattcccgt acttcagagg
     1381 cgtacttgat ggcgccgaat cggcaggcat cggtcctctc tcgctcggaa tatcgctcaa
     1441 aacacagctg cgaaacctgt tcgtagtagg cgtctaaacc ttcgcttgag aagccggtaa
     1501 ctgtgcggat tgagacctcg ccagcgcgat ttcgttcaga gatctctacc aactgtaggt
     1561 caattccggc caccgactgt tcggcagcga tttgcacacc ttctcgacat gccctgaaga
     1621 gatcacgggt cgaacggttg gcttttgggt attccgactc gcatttttga agagttgcat
     1681 aaatcgtctt ttgtggggcg caaccgaagg ccaatatgct tgcagccatc gaaaaggcca
     1741 cgattaaccc tttagaatca aggaatcggc gcttataaaa atgtacattt gggaaagatg
     1801 cgtgcaaaag acccagaccc ttcaaatcaa tgttttgtat aaaggctagg taacacctcg
     1861 atattaaagg gccaagcgga aactttccct tcttttgagg tgccggtgac tcagtccaaa
     1921 agcaccactt cagtacgagt cctcttaaaa ctcctaggga acaggcctgg ccgatatctt
     1981 gcgatatctc aatttgccag cccggctttt cctggcgctc acacagtggg cgcggggagc
     2041 gctacccggc tacgagaggt tttatgcaaa acgagagacg aattctagtg gtcgactccg
     2101 accgcgattt cactcaaaat ttagttcgcc aaaataagtc gagtcgaatc gatctagtta
     2161 ttgcgcacaa tcgagccgat gcgttaactg ccatcgccca tgcccggggc ccttttgcgt
     2221 cggtcctggt caataagaag ctcgccgatt acggtaccta ttcggtggtc cgtcaggttc
     2281 acgtccgcca tcttggaacg ccagtttatc tgctcagcga agaacccatt gaaaatgaag
     2341 atctaaagga aaacttaaac gcccttggta ttcgacaagt gctcccgaaa ccccttactt
     2401 acgaagagct cgtcaaacag ctcgaatcgg tgttggtggg atttaaaagc gtcgcccctt
     2461 cacaaggaat caaagattcc tcaatggtag gtaccgagct cggagccccg gaaaacgact
     2521 tcacgccgat cagagcccaa cattttctat caggccaccg ttctcacttt agtgtctacg
     2581 tgcagattcg aagtaatcgc tttctcaaaa ttctgaatgc cggcgatgtg ttcgacgctc
     2641 gccgactgga acaatatttg gcccgtggca ttcagtggtt ccatattcgc aaagacgcac
     2701 acagatccta tttagaatac tgcgactttg tttcgaaaaa attgatcgaa catggcatgc
     2761 attcaatcga aacacagcgc gaccaagtgc tcaatctcgg cgaggaaact ctacgatatt
     2821 ttggacacgc aggcgtatct ggtgacctga ttcactacgc cgttaatttt gtgcaggact
     2881 gcaaccaatt aacaaaaaaa ctgcaactag atcgccaccg ggaaatcggg gcattcatga
     2941 ataatttggt agagtgggaa cacagcgtga gcgtcgccct agtgacttcc ctactaatgc
     3001 ggggcttacg cattgaaaac gaacgagttc agaactctat cggccttgca gcccttttgc
     3061 acgacatcgg gaaaattcaa acttgcccaa gcctaataca cgaggacctc acccgcatga
     3121 cggagcaaga aattgcgacc tttaaagacc acgcgtgggc cggcgcgacg atgatagcgg
     3181 gggtaccggg gattgattcg accacagtgc aggcggtcgc ccagcaccac gaacgacgca
     3241 atggcaaagg gttccctctc ggtttaggag ctggaaaaat caaccgcgtg gcggaaatcg
     3301 taggactggc agacgaatac accaacctaa ttcataaaaa taaagtgttg ccagaaatta
     3361 caaaaattca ctttctgcaa gatactttgc tagacggatt ttcaaccgag gtggtggaca
     3421 gtctttgcgt gttatttctt cgaaactagt gaatcaactt cggcgaacaa taggtattc
//
LOCUS       DAOMXO010000196         3476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14991_length_3476_cov_4.461269, whole genome shotgun sequence.
ACCESSION   DAOMXO010000196 DAOMXO010000000
VERSION     DAOMXO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14991_length_3476_cov_4.461269"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLH57_05175"
     CDS             complement(<1..564)
                     /locus_tag="PLH57_05175"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRK02036.1"
                     /translation="MSATSGTTPPNDIILVLTVRSQKTPGEKVFRLHKRRVVLGSSTS
                     SDVRLEDLSVSPVHAILETSGIDKKPVIYDLASDSGVLVNGKPVVQATLEMNDKVQVG
                     PFMLSVRAHTMADLPQTPQFVRESQEGQKLFVDEKEDFTPLILEDESEVYDIFDHRPA
                     QKRSLQIVMFFDDTILDVEHFQKKKKII"
     gene            complement(561..920)
                     /locus_tag="PLH57_05180"
     CDS             complement(561..920)
                     /locus_tag="PLH57_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02037.1"
                     /translation="MISLVCLGLASESGAQEKRSRFRSGTEVRGQTNATPKAAPKRSQ
                     IKNYSKKYNVVEEDGKKVLYKKRNVVEFDGSMIEGEIRNPNDFYFVHRPEENFGSLLQ
                     KRKNFHKEMLRDTVMIR"
     gene            complement(935..>3476)
                     /locus_tag="PLH57_05185"
     CDS             complement(935..>3476)
                     /locus_tag="PLH57_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02038.1"
                     /translation="ALATTIRVRGYLRSRASEVHKIARNKKSKEHYAQAASLYGLFIK
                     AFGRVAESEKEKNDLNQVRIYRADCLLAASREDAALEELEIALNETGESKLKREAGAT
                     ALNLLLKRLDRGKSDSESEKRFERLAESFEDKFPKDPLVSEIRYKRARLAARKSGSEG
                     LSSDARDALNELIEKYPNRPESLLAAQDLVDDLRKRKEDDEASELAGKLLANSALMAT
                     DQKRELKKYLEAIVARRRFERVQNLEGNQNHESAAREYESLATQNKNNSEVYFKSLNN
                     AAINYEKAGLSAEARRVFLKLHREFNKSNVGRTGLKRLGDTLLYEAKLSEAGAAYREL
                     SEINSFTASEQASFGELAATIYWDLKDHGNSYILMQKLQRDHCSGKVNPSMAAKCRNW
                     EFMQGELLLDAGKHQDALSQFRKIADSKARFGSRRAESAYRAAMIYERFAERSKSNAM
                     LEKAAGIATGGKEPWQARERNYAARSAFQLIEPVFGTFDSIKLELPEAKLKANTKRKL
                     SELERLVVRYNQIVARGDGEWGMAALERLGRAFYDFSRELDQAPVPTTLTPDQQTQYR
                     REIQSIAGPMAQKARESFVQAHALGVRLKIVTETFDRVRVTLGYFAPTKTPPAQYFRS
                     DTSSESYLGLQFLGREIEDFGARLKANAKDASAWIAFGLKAMNDGHTRLAHAFFEQAV
                     SLAGKSHQTAAIGWNNLAVFAAVNNELAQAQAAFEKAVDLADLNETIQLNFGKFLIYY
                     GQYARGGALLNKLKARLGDTFSEEAEIARLVAQLGQGELRAAKIALEKKSAKSSDSPT
                     LWYNWSVASILSGDTDDQNDGRELLKERRNQLPKDLQWIADGLIGYRK"
BASE COUNT          795 a          886 c          779 g         1016 t
ORIGIN      
        1 gataattttt ttctttttct gaaaatgctc gacatcgaga atcgtgtcat cgaaaaacat
       61 aacgatttgg agactacgct tctgggcggg gcgatgatca aaaatatcat aaacttctga
      121 ctcatcttct agaatgagag gagtaaaatc ttctttttca tcaacgaata gtttttgacc
      181 ttcttgggat tcccgtacga attgcggtgt ctgcgggaga tctgccatcg tgtgagcacg
      241 cacgctcaac ataaacggtc ccacttgtac tttgtcgttc atttctagag tagcttgtac
      301 gactggtttt ccgttaacca gtacacctga gtcgcttgct agatcgtata tgaccggttt
      361 tttatcaatg ccgctagtct cgagtattgc atgaaccgga cttactgata aatcttcgag
      421 tcgcacgtcg ctggaggtcg acgatccaag tactacacgt cgtttatgaa gacgaaacac
      481 tttctcaccg ggagtctttt gactgcgtac tgttaaaact aaaattatat cgttgggtgg
      541 agtggttccg ctcgtagcgc tcatcgtatc atcaccgtgt ctctcaacat ttctttatga
      601 aaattctttc tcttctgtaa aagcgacccg aaattttctt ctggtcgatg cacgaaataa
      661 aaatcgttag gattccttat ttcaccctcg atcatcgaac cgtcgaattc aaccacgttt
      721 ctctttttat agagaacttt ctttccgtct tcttcaacaa cgttatattt tttggaataa
      781 ttttttattt gcgaacgttt aggagcagcc ttgggcgtag cattggtctg accccgtact
      841 tccgttcccg agcgaaatcg acttcgtttt tcttgagcgc cgctttcgga ggccagtccc
      901 aggcacacga gacttatcac agcgcctaga aaagctattt tcggtaaccg atcagaccat
      961 ctgcaatcca ttgcaagtcc tttggcagtt gattacgtcg ttctttgagc agttcgcggc
     1021 cgtcgttttg atcgtccgta tctccggaaa gtatcgacgc cacagaccaa ttgtaccata
     1081 gcgtcggaga gtcggaactt tttgccgact ttttctctaa cgctattttc gcagcgcgca
     1141 actccccttg tcccaattga gctactagcc ttgcgatttc cgcttcttca gaaaatgtgt
     1201 cgccgagccg cgccttaagt ttattcaaaa gcgcgccgcc acgtgcgtat tgcccgtagt
     1261 atattaagaa tttaccaaaa ttcagttgaa tcgtttcatt taggtcagca agatcaaccg
     1321 ctttttcaaa cgcagcctgc gcttgcgcaa gttcgttatt cactgccgca aacacagcga
     1381 gattattcca accaatcgct gccgtttggt gggacttccc tgccaaactg actgcttgtt
     1441 caaaaaatgc atgggcaagg cgggtgtgtc cgtcgttcat cgcctttagg ccaaacgcga
     1501 tccaagcaga ggcgtctttg gcattagcct ttagccgcgc cccaaagtct tcgatttctc
     1561 ggcccaaaaa ctgtagccct aaataagact cgctcgacgt gtcggaacga aaatactggg
     1621 ctggcggagt tttggtgggc gcaaagtacc caagagtcac cctcacgcga tcaaatgtct
     1681 cggttacgat tttaagtcgc acccctaacg cgtgggcctg aacaaaagac tcgcgagctt
     1741 tttgagccat tggtcccgcg attgattgaa tttcccggcg gtattgcgtt tgttgatccg
     1801 gcgtcagcgt cgtcggaaca ggcgcctgat caagctctct gctgaagtcg taaaaagcgc
     1861 gtcccaatct ttcgagtgcg gccatgcccc actcgccgtc tccgcgagcc actatttggt
     1921 tgtagcgcac gaccaaacgt tcaagttccg aaagtttacg cttcgtgtta gcctttaatt
     1981 tggcttccgg taattctagt ttaatagagt cgaaggttcc gaacactggc tcaatcagct
     2041 ggaatgccga gcgcgccgca tagtttcgct cccgcgcttg ccacggctcc ttaccaccgg
     2101 tagcaatgcc ggccgctttt tctaacatag cattggactt cgatcgctcg gcaaagcgtt
     2161 cgtatatcat ggcggcgcga tacgctgatt cagcgcgccg cgacccaaaa cgggccttcg
     2221 aatcggcaat tttgcgaaac tgagacagag catcttggtg ttttcccgca tccaggagaa
     2281 gttctccctg catgaattcc caattacgac atttggcggc catgctcggg tttactttac
     2341 cggaacagtg atcacgctgt agcttctgca ttaatatgta actattgccg tggtctttta
     2401 aatcccaata aatagtggcc gcaagttcgc caaaactcgc ttgttcgctt gcggtaaaac
     2461 tattgatctc cgaaagttca cgataggcgg cgcccgcttc agatagtttt gcttcgtaaa
     2521 gtaaagtgtc gcctaagcgt ttcaatcccg tacgaccaac atttgattta ttgaactctc
     2581 ggtgaagttt caaaaacact cgcctagctt cagcgcttaa cccggctttt tcataattga
     2641 tggcggcatt attcagggac ttaaaataca cttcgctatt atttttattt tgcgtcgcta
     2701 gggactcata ttcacgggcg gcgctttcgt gattttggtt cccttcaaga ttttggactc
     2761 gttcaaatct tctacgggcg acaatggctt ctagatactt ttttagttcg cgcttttgat
     2821 cggtcgccat taacgcggaa ttagctaaca gtttaccggc caactcagag gcctcatcgt
     2881 cttcttttct cttgcgtaga tcatcgacca agtcctgggc agcaagcaaa gattccgggc
     2941 gattggggta tttttcaatt aattcattca gtgcatctct agcgtcacta gaaagacctt
     3001 ccgaaccaga ttttcgtgcg gccagtcgcg cgcgtttgta acgaatttct gataccaagg
     3061 ggtctttagg aaacttgtct tcgaaactct ccgcaagacg ttcgaaccgc ttttcggatt
     3121 cggagtcgga cttacctcgg tctagccgct tgagcaacag attcaaagct gtcgcccccg
     3181 cctcgcgttt taatttagac tctccggttt cgtttaacgc aatttccagc tcttcgagcg
     3241 cagcatcctc tcgactggcg gccaataaac aatcggcccg gtagatgcgc acttggttta
     3301 gatcattttt ttctttttcg ctctcggcga cacggccaaa tgctttgata aaaaggccgt
     3361 agagggacgc ggcttgcgcg tagtgttcct ttgatttctt attacgggca attttatgaa
     3421 cttcgctggc tcgactgcga aggtatcccc gaactcgaat agtagttgca agagca
//
LOCUS       DAOMXO010000197         3475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_14995_length_3475_cov_4.528070, whole genome shotgun sequence.
ACCESSION   DAOMXO010000197 DAOMXO010000000
VERSION     DAOMXO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14995_length_3475_cov_4.528070"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..390
                     /gene="rpmB"
                     /locus_tag="PLH57_05190"
     CDS             139..390
                     /gene="rpmB"
                     /locus_tag="PLH57_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRK02039.1"
                     /translation="MARACEFCGKRPTTGNLVSHSNIKTRTRWLPNLKKVKHFAKGIT
                     KTVYSCTRCIKSGLITKPPVRDRAAMLAAAAAKSAAAAK"
     gene            467..1261
                     /locus_tag="PLH57_05195"
     CDS             467..1261
                     /locus_tag="PLH57_05195"
                     /inference="COORDINATES: protein motif:HMM:NF021527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HRK02040.1"
                     /translation="MARCIQQLIIALWCALTLAPMTSLEASEYFRPLRQGHSCVAMIH
                     GILPQIAVYNFSGFTEPLAPYFDNLPIDEPLAALGKPFRRRRFSRFRISAFDHSIERI
                     PGQALVQSLENNRLFGEVVRVFEPLESAMVDSSDFKKLVRDFAGALPGNAFDYEVGVH
                     QIRTSIEPKKMGKEITPVPEGRHRDGFDFVGALVIKRHNIRGAQTILALSKTAPPKFK
                     TTLLPGQLLIFNDREYFHNATVMKSRNHEQGIRDVFVLTAEKKERR"
     gene            complement(1269..1649)
                     /locus_tag="PLH57_05200"
     CDS             complement(1269..1649)
                     /locus_tag="PLH57_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRK02041.1"
                     /translation="MAAKEIRTDNAPAPVGPYSQAIEVNGTVYCSGQIALDPKSGQMI
                     GNTPVEQLQQLMKNVDAVLAAAGLKFENIVKTTIFLTDMKAFPDVNAAYEKILKGHRP
                     ARSTVEASALPKGALVEIECIAVR"
     gene            complement(1639..>3475)
                     /locus_tag="PLH57_05205"
     CDS             complement(1639..>3475)
                     /locus_tag="PLH57_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRK02042.1"
                     /translation="DQHGTAIILGAAFLNALELTGKKAEKVKCVFVGAGAACIASANM
                     FLLLGVKPENMIMTDIDGVLHKKRVEGMNPYKEKFARDTKLRTLDEAIKDADVFVGLS
                     AKGVMTKEMVAKMGKNPMIFAMANPEPEILPEDVAAVRKDAIMATGRSDYPNQVNNVL
                     CYPFMFRGALDVHATGINEEMKMAAVKALAALAKEEVPESVSRAYGGKAFRFGLDYLI
                     PKPFDNRVLLWVAPAVAKAAMDSGVAQKKINVQEYRQQLEHLLGATFTVMRSIKNRVK
                     KGQRSVSAGTDGGSRLKTLVFPEGEHPKILKACAQLIDDSIAEPILLGRRDRITNVIS
                     QLGLDAALAGVQIVDPAQSSKLDEYAASWSKMRERKGMTPRLARELLVRRNTFGCMMV
                     HMGDADGLLNGISQSYPETIKPAIQIVGARAGSKLAGIYMLILKRRVLFFADTTVNIE
                     PSPEDLADIAIMTADLSQHFVDEDPRVAMLSFSNFGSARHPLAERVSRAVEIARAKRP
                     NLVLDGEMQADTALVPEILSADYPFSCLKEPANILIFPDLQSGNIAYKLMMRLGGAEA
                     IGPILVGMNKPVNVLQQNSDVQDVVNMATITVIEAQQMEREKNGS"
BASE COUNT          824 a          921 c          816 g          914 t
ORIGIN      
        1 taccaacact atagcaaggg gccccgcccc cccgcccgtc ttcccccttt tttagtatgg
       61 cgcacaccgc ttgcattttt ttaaagcttc aagtaggttg ccggcactac agattttctt
      121 tcgaagagga atcgaaccat ggcacgagcc tgcgaatttt gcggaaaacg cccaacaact
      181 ggaaaccttg tttctcactc caacatcaag acccgcacgc gttggttgcc aaatcttaag
      241 aaggtaaaac acttcgctaa aggcatcacg aagactgttt actcctgcac tcgttgcatc
      301 aaaagtggtc tgatcaccaa accaccagtt cgcgaccgcg ccgccatgtt agccgctgcg
      361 gctgctaagt cggctgctgc tgcgaagtaa ccctccgcta cccaacaaaa ggtcttattc
      421 ggacccgtcc tttacctgtc ttttttggta tgttaaagag gcggggatgg ctcgttgtat
      481 ccagcaactt attatagcgt tgtggtgtgc gctcaccctg gcaccgatga cgtcgcttga
      541 ggcgagcgag tattttcggc cgctaagaca ggggcattca tgcgtggcta tgatccacgg
      601 aattctgccc cagattgccg tctacaactt cagtggcttc accgaaccat tggctcccta
      661 ttttgataac cttccgatcg acgaaccgtt agccgctttg ggaaaaccgt ttcgacgtcg
      721 ccgcttttca cgatttcgga tcagcgcgtt cgaccattcg atagaacgaa taccgggtca
      781 ggcacttgtt caaagtctgg aaaataatcg attgttcgga gaagtcgtcc gagttttcga
      841 accgcttgaa tcggctatgg tggactcctc ggattttaaa aaattggttc gggacttcgc
      901 cggtgccttg ccaggaaatg ctttcgatta cgaagtcgga gtacaccaaa ttcgcacttc
      961 tatagaaccc aaaaaaatgg ggaaagaaat aactcctgtt cccgagggac gtcatcgcga
     1021 cggatttgac tttgttggag cccttgttat taagcgccac aatattcggg gcgcccaaac
     1081 tattctggcg ctttcaaaaa ctgcccctcc aaaatttaaa actacgcttt tacccggcca
     1141 actattgatt tttaatgacc gggaatattt tcataacgcg acagtcatga aatctcgcaa
     1201 ccacgaacaa ggaattcgtg acgttttcgt attaacggcc gaaaaaaaag agcgccggtg
     1261 atttgatttt aacgaaccgc aatgcactcg atttctacta gtgctccctt ggggagggcc
     1321 gaagcttcaa ccgttgaccg ggcgggccga tgacctttta aaattttttc gtaagcagcg
     1381 ttcacgtcgg gaaacgcttt catatcggtt agaaaaatcg tggtcttcac aatattttcg
     1441 aacttcaaac cggcggccgc gagtactgcg tcgacgtttt tcataagctg ctgtagctgc
     1501 tctaccggcg tgttccctat catttgacca gatttgggat cgagagcaat ttggcccgag
     1561 caataaacgg tgccgttaac ctctatcgcc tgcgaatacg gtccgactgg ggctggggca
     1621 ttgtcggttc gaatctcttt agctgccatt tttttctcgc tccatttgct gagcctcgat
     1681 cacagtgatc gtcgccatgt tcaccacgtc ttgcacgtcc gagttttgtt gtaacacatt
     1741 tacgggcttg ttcatgccca ctagaattgg accaattgcc tcggcgccac ccaaacgcat
     1801 catgagttta taagcaatat tccccgactg cagatcggga aatattaaaa tattagccgg
     1861 ctctttaaga cagctaaacg ggtagtccgc gcttaaaatt tcgggcacga gggcggtgtc
     1921 ggcttgcatt tcaccatcca gcactaaatt ggggcgttta gcccgtgcga tttctacagc
     1981 tctggaaact ctttccgcga gcggatgacg agccgatccg aagttcgaaa aactaagcat
     2041 tgccactcgc ggatcttcgt cgacaaagtg ctgagacaaa tcggccgtca tgatggcgat
     2101 atcagcaagg tcctcgggac ttggttcaat attgacagtg gtatcggcga aaaacagcac
     2161 gcggcgtttt agaatcaaca tgtaaatacc cgcgagttta ctacccgctc gcgcgcctac
     2221 aatctgaatc gccggtttta ttgtttccgg ataactttgt gagattccgt taagtaggcc
     2281 atcggcatcg cccatgtgca ccatcatgca cccgaaggta tttcgacgca ctagcaactc
     2341 ccgagcaagt ctaggggtca ttcccttacg ttcgcgcatt ttggaccacg acgctgcata
     2401 ttcgtcgagt ttcgatgatt gcgcggggtc tacgatctgc acgcccgcta aagcggcgtc
     2461 gagtccaagt tgcgatatta cattcgtgat tcggtcccga cgtccgagca aaatcggctc
     2521 cgcaattgaa tcgtcgatga gttgcgcaca agctttaaga atcttcgggt gctctccctc
     2581 aggaaacacc aaagttttaa ggcgtgatcc gccatcggtg cccgcactga cggagcgctg
     2641 gcctttttta acccggtttt taatcgatcg catgacagta aaagtcgcgc ccaacagatg
     2701 ctcgagttgc tggcgatatt cctgaacatt gattttcttt tgggccacac cgctgtccat
     2761 tgcggccttc gcaacggcgg gcgccaccca aagcaaaacg cggttatcaa atggtttggg
     2821 aataagatag tccaacccaa atcgaaacgc cttgccgcca tatgcgcgag aaaccgactc
     2881 gggcacctct tctttggcta gagcagccag cgcttttacg gcagccattt tcatttcttc
     2941 gttaatgccg gtggcatgca cgtcgagggc accgcgaaac atgaacgggt aacaaagtac
     3001 gttatttact tgattgggat agtcgctgcg ccccgtggcc atgatcgcgt cttttcttac
     3061 ggcggccaca tcttcaggca aaatttcggg ttccggattt gccattgcaa aaatcattgg
     3121 atttttgccc attttggcaa ccatttcttt tgtcatcacg cctttagcgg atagacccac
     3181 gaacacgtca gcatctttta tggcttcatc caaggtgcga agtttcgtgt cacgcgcaaa
     3241 cttttcttta taggggttca ttccctcgac gcgtttttta tgaagtacgc cgtcgatgtc
     3301 ggtcataatc atattctcgg gttttacccc aagtaataaa aacatgttcg cacttgcgat
     3361 gcaggcggct cccgccccaa caaaaacaca cttaactttt tcagcctttt taccggtcaa
     3421 ctcgagcgcg tttaaaaacg ctgcaccaag gatgatagcg gtaccgtgtt gatcg
//
LOCUS       DAOMXO010000198         3457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15104_length_3457_cov_5.264256, whole genome shotgun sequence.
ACCESSION   DAOMXO010000198 DAOMXO010000000
VERSION     DAOMXO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15104_length_3457_cov_5.264256"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..468)
                     /locus_tag="PLH57_05210"
     CDS             complement(106..468)
                     /locus_tag="PLH57_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HRK02043.1"
                     /translation="MARFRAHVIIKRMVPETPDIHFDDDRFWSLNDDGTLVIGVGASG
                     LEELGRLQSVKFPRVNQECGEGDILCTLLGDDGELQLVAPTSGVIDAINNAVMQDIEI
                     VNDDPYSEGWLVRLTESS"
     gene            complement(518..>3457)
                     /locus_tag="PLH57_05215"
     CDS             complement(518..>3457)
                     /locus_tag="PLH57_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02044.1"
                     /translation="DGDAPSGQAGATGGQAGADGDAPSGQAGATGGQAGAGSDQVDAS
                     KDVEPAVIEVLQKDLDQKPRDGRRAYIPGPYSPDQVVAVVDGPNGEKVNLIDVNGDGY
                     PDAYDRGADGKADGPYSDADRNYDGVVDEEEKKRYLKENPDAGGDLNKDGKVDNIEKD
                     YDLNGDGVVDLNEKERFAEEQGRRARDLNKDGKYDNREETFDVNKDGIIDEKEKKADK
                     NGDGIVDEEERKKFAKENNLDDKDNDGKVSAKDESFDLNKDGVADHKERKADVNNDGV
                     VDENEQKKFRDRNPEYGRDTDGDRREDAYEVAADKNKDGVVSPEELADYKKNNPDAGL
                     DINGDGEVSEVERAHDTNGNGVIDQEERESYARSHPNEDVDISKSGDDGKTSQGQGGV
                     HSDAAEPKSDEEGMGNDSKPIAKKNKVARCFSKDMFGEQTETSSDEVRNYCIDIKRRD
                     DALTKAMERAQAECAKVTQLPISYDQIVTACQVKKVNRIDMNLWSQPLVFNESGFGFS
                     FQCQTRATQCDDRARIIPYYGEAAYQGVVSGDVKVPIVDSNSEDARYIKYANRWATQQ
                     IAFLESSKGVYAAGKHCLQKTWTPDATSAQCKRDDVGREFTYDTQFDYHSRIAGSESV
                     RMTCEISCSCSDFSCIAKPVKGVQLPEDIHFNQARDSKVPVVEAYVSAYSTDEIVQIG
                     NSQRDSGASSPDSHSGAVGETSQRVKPAEAVRAMESDSLLLDKEYLNRRLAARQKTEV
                     RAKKYCSVSVERVPQYLAKNGKDPASPGNYFCGGQSEKIQLLAYVKEDLSGTMAKFSV
                     KEDGFEFSYQCIYDCAKKECTSKSTDAKAFNGECAFTSYRTEVGSKTKDIQTRNQYLA
                     AKNHDAVVLWPKMRSTCDVKKPKGLPAIIQESCGGNSQGAPRVHVFRDRSVHRTAEGG
                     DLTGSVTNGAGLLFTCEYECDCKKKSCELPNNRGPVLIDLADGPDGDWTKDVELRRNK
                     PSTWDKN"
BASE COUNT          709 a          953 c          773 g         1022 t
ORIGIN      
        1 tgtttggttc gctcaatgac cgacttcgaa gtttcgccgc cattgttctt ttttttactg
       61 tttcgagatg gcgttacagt tttttttccc atcgcatcgt gctttttagg aactctcggt
      121 taaacgaacc agccagcctt cagaatacgg atcgtcgttg acgatctcaa tgtcctgcat
      181 caccgcgtta ttgatggcat cgatcactcc gctcgtaggt gccacaagct gcagttcccc
      241 atcgtcgccc agcagtgtgc acagaatatc accttcacca cactcttgat tgaccctcgg
      301 gaacttgacc gattgcagac ggcccagctc ttcaaggccg ctcgcaccga caccgatgac
      361 cagcgtacca tcgtcattga gcgaccaaaa ccgatcgtcg tcaaagtgaa tgtcgggagt
      421 ctcaggcacc atacgtttga ttatgacatg cgcacgaaaa cgcgccaaag aaattacttc
      481 aaaagagacg aatagttggg acaagtcccg agcgatctta gtttttgtcc caagtactgg
      541 gtttgttgcg ccgcaactcg acgtctttgg tccagtcacc atcgggtcca tcggctaaat
      601 cgattaagac cgggccccgg ttgttcggta gctcgcatga cttcttctta cagtcacatt
      661 catactcgca ggtaaacaaa agtcccgcgc cgtttgtcac cgaaccggtc agatcgccac
      721 cttcggccgt gcggtgcacc gagcgatcgc gaaaaacatg aacccgtggg gcaccttgac
      781 tatttccacc gcaactttct tgaataattg caggcaaccc ctttggtttt tttacatcgc
      841 aagtggaacg cattttgggc caaagcacga cggcatcatg attttttgct gcaaggtact
      901 gattgcgtgt ttgaatatct ttggttttgc tgccgacttc agtgcggtag ctagtaaatg
      961 cacactcgcc attgaaggcc ttggcgtcgg tggacttcga cgtacattct tttttggcac
     1021 aatcgtaaat gcactgatag ctgaactcga atccgtcttc tttgacgctg aatttcgcca
     1081 tcgtgcccga aagatcttcc ttcacgtagg ctaagagctg tattttttca ctttgtccgc
     1141 cgcaaaaata atttcctggc gaagccggat ctttgccatt tttggccaaa tactgcggca
     1201 cccgttcaac tgataccgaa caatattttt tggccctaac ttcggttttt tgccgagctg
     1261 ctaaacgacg gttcaggtat tccttgtcaa gcagcagact gtcggattcc atggctctta
     1321 cagcttctgc gggttttact ctttggcttg tttctcctac cgctccagag tgcgagtcag
     1381 gactggaggc tccagagtca cgttgagaat ttccaatttg cacgatctca tcagtcgagt
     1441 atgcagacac atatgcttca actactggaa ctttcgaatc tctcgcttga ttaaaatgta
     1501 tatcttccgg caactgcact ccttttaccg gctttgcaat gcaggaaaaa tcggaacacg
     1561 aacaactaat ttcacaggtc attcgtacac tttcagaacc cgcaattcgc gaatgatagt
     1621 caaattgggt gtcgtaagtg aactcccgac ctacgtcgtc acgcttacac tgagcactcg
     1681 tggcatctgg agtccacgtt ttttgcaaac aatgtttacc ggcagcgtaa acgcccttcg
     1741 acgactcgag aaacgcaatc tgttgtgtgg cccaacggtt agcgtatttt atgtagcgag
     1801 catcttcaga atttgaatcc actataggta cttttacatc gcctgaaacc acgccctgat
     1861 aggccgcttc gccatagtaa ggaatgattc tcgcacgatc gtcgcactga gttgcgcgcg
     1921 tttggcattg gaaagaaaac ccaaagccag actcgttaaa tacgagcggt tgtgaccaca
     1981 aattcatatc gatgcgattc acctttttca cttggcacgc ggtcacgatc tgatcatacg
     2041 agatcggcag ttgagtgacc ttggcacatt cggcctgggc acgttccatt gctttagtta
     2101 atgcgtcgtc gcgccgctta atatcaatgc aatagttccg aacttcatcg ctcgaggttt
     2161 cggtttgctc gccaaacatg tccttggaaa aacaacgagc cactttgttc tttttcgcta
     2221 tcggcttgga gtcgttgccc attccttctt cgtcagattt aggttcggcc gcgtccgaat
     2281 ggacccctcc ctgcccttgg gaagtcttac cgtcatctcc agatttagat atgtctacgt
     2341 cctcgttcgg gtgggatcga gcataacttt cgcgttcttc ttgatcgatc actccgttac
     2401 cattggtgtc gtgggcccgc tctacttcgc tcacttcacc atcaccgttg atatcgagcc
     2461 cagcgtctgg gttgttcttt ttgtaatcgg ctaactcttc gggcgacact acgccgtctt
     2521 tatttttgtc ggccgctact tcgtaggcgt cttcgcgacg gtccccgtcg gtgtcgcgac
     2581 cgtattccgg attacgatca cggaactttt tctgttcatt ctcatccacg acgccatcgt
     2641 tattcacatc ggccttgcgt tctttgtgat cggcaacacc gtctttatta aggtcaaacg
     2701 attcgtcttt agcggaaact ttaccgtcgt tgtctttgtc gtctaaatta ttttctttcg
     2761 cgaatttctt acgttcttct tcgtcaacaa tgccgtcgcc gtttttgtca gccttctttt
     2821 ctttttcgtc gataataccg tctttattga cgtcgaacgt ttcttcgcga ttgtcgtact
     2881 taccgtcttt atttagatcg cgcgcacgcc ggccttgctc ttcggcaaag cgttcttttt
     2941 cgtttaaatc caccacgcca tcgccgttta aatcgtagtc tttttcgata ttgtctactt
     3001 taccgtcctt gttgagatcg cctccggcat ctggattttc tttaaggtag cgtttttttt
     3061 cttcttcatc gaccacgccg tcgtagttgc ggtcggcgtc gctgtacgga ccgtcggcct
     3121 tgccgtcggc acctcggtca taggcgtcgg ggtaaccgtc gccgttgaca tcgatcaaat
     3181 tcactttttc gccatttggt ccatcaacca ccgcgactac ctgatccggg ctgtaaggac
     3241 ccggaatata cgctctgcga ccgtcgcgag gcttctgatc taaatctttt tgcagtactt
     3301 cgataacagc tggctctaca tctttcgaag catctacttg atcgctgcca gccccagctt
     3361 ggccgcctgt agctcccgct tggccgcttg gagcgtcgcc gtcagcccca gcttggccgc
     3421 ctgtagctcc cgcttggccg cttggagcgt cgccgtc
//
LOCUS       DAOMXO010000199         3455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15119_length_3455_cov_4.655882, whole genome shotgun sequence.
ACCESSION   DAOMXO010000199 DAOMXO010000000
VERSION     DAOMXO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15119_length_3455_cov_4.655882"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..759)
                     /locus_tag="PLH57_05220"
     CDS             complement(94..759)
                     /locus_tag="PLH57_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02045.1"
                     /translation="MAILICAATTTEYNAVQRALRKTGAIHKFNLLRTGMGARAAQAA
                     LLNELSNHARTPDLVISTGFAGGLSEKFEIGSWVYDASTNSVRPPNSWIEILGAVPVL
                     VRNAEKVVHPGSNMNLSEAVDMESATLSKICSDRKIPFAVLRLISDNPRAPLPTFVGD
                     WMELSNAETLTAKSAMFVTAFTRTIRQPLRVWGLVRNARRWRRDLHAGWVRLGYRVGT
                     HGL"
     gene            complement(747..1559)
                     /locus_tag="PLH57_05225"
     CDS             complement(747..1559)
                     /locus_tag="PLH57_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HRK02046.1"
                     /translation="MLEQVLEVMRLFWGSVVLRPYVYLFFLCYLVFSIRHMGVFRSLI
                     FTASTYLIAFGCEYSSTRNGFPFGIYTYIDTTRNQELWISNVPFWDSLSFVFLSYFSW
                     LLAGFIVANGRDLNKGLTARVTPWFGGVLMTLLDVVIDPVALQGHKWFLGQIYFYPHE
                     GFYFGVTAANFAGWFFVGFATQWVFIWLLRILPGLAKPFRTPDWNFIWGVYAVYAGVF
                     VFNLAITIAIRDYALAMASTAVMVVTLTFVGFFLRDKTRSLNRVPLRSIWQS"
     gene            complement(1552..>3455)
                     /gene="shc"
                     /locus_tag="PLH57_05230"
     CDS             complement(1552..>3455)
                     /gene="shc"
                     /locus_tag="PLH57_05230"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HRK02047.1"
                     /translation="AAKKAIDYCISIQSKDGHWCAELESNATITSEYVFLQHALGLDL
                     SKKKNAICRYFFGNQKADGSWGIASNHPGDLSTTAECYLALRLLGISTNDERLKKAEH
                     YILAQGGLERVRIFTRINFAMFGLWPWSAIPVIPPEFIFLPPKSPVNIYSLSSWARGT
                     MVPLFMIFHHKPIFALPNGRSASNDWLDHLWRNPTQKNIPYTDPLVEILRKHGTGWKS
                     FFSASDVFLRTYERYKPRWLRAFAIKKCEEWVLERQEKTGDWAGIFPPMYNGVLALYL
                     HGYSLDSKEVKGGLEAIERFAIEDEQGFRVQACVSPVWDTVLTMIGMIDCGHDGKDEK
                     LLRAREWIKQRQLLVDYGDWKVYNPKLRSGGWAFEYENSWYPDVDDTAAVIIGLLKQD
                     RDFSKSLTVQRAVEWTVGMQNRDGGWAAFDINNDKIFLNEIPFSDMDSLCDPSSPDVT
                     GRVLEALGILGGRKFEKNIKRGVEYLRKTQEKEGSWFGRWGVNYVYGTSNVLCALSRL
                     KNVRANDPMIVRALDWLKSVQNADGGWGECLESYSNKALMGKGQSTASQTAWAVMGLL
                     AYLPATDESIRKGIEWLITRQKSNGNWDEEEFTGTGFPNHFYLRYHLYRQFFPMMAIG
                     RFLQRASNA"
BASE COUNT          980 a          879 c          741 g          855 t
ORIGIN      
        1 tgtactgcta taacactacg cgttaatcta agcaaggtgg gggtaaactt ttttattcgg
       61 gtttaatggg cctaggtaat cggcttttat gccctataat ccatgggttc cgacgcgata
      121 cccaagccga acccaacccg catgaaggtc tcggcgccac cggcgggcat tccgaactag
      181 accccagact cttaacggct ggcgaatggt ccgtgtaaac gcagtgacga acattgctga
      241 tttcgcggta agcgtttcag cattcgagag ttccatccaa tcaccgacga aggtcggcag
      301 cggtgctcgt ggattgtctg aaattaatct caaaaccgcg aacgggatct ttcgatccga
      361 gcaaattttc gacagcgtcg ccgactccat gtccactgct tcggataaat tcatattgct
      421 tccggggtgt acgacttttt ccgcgtttcg caccaagact ggaaccgcgc ctaagatttc
      481 aatccaactg tttggtggac gcacactatt ggtcgaagcg tcgtaaaccc aactacctat
      541 ttcaaacttt tcagataggc ccccggcaaa gccggtagaa atcaccaagt caggggtgcg
      601 ggcgtgattg ctcaattcgt ttaacagcgc cgcctgagct gctcgcgctc ccataccagt
      661 tcggagtaaa ttaaatttat gaatagcacc tgtcttgcgt agggcccgct gaacagcgtt
      721 atattcggta gtggtcgcag cgcaaattaa gattgccata tggaacgtag agggactcgg
      781 ttgagcgaac gcgtcttatc ccgtagaaaa aagccaacaa acgttaaagt tacaaccatt
      841 accgcggtac tggccatggc caaagcgtag tctcttatcg caatggtaat cgccaagtta
      901 aatacaaaca cgccagcgta aaccgcgtac acaccccaaa tgaaattcca atccggggtg
      961 cgaaagggtt tcgcgagacc aggtaagatc cgcagtagcc atataaatac ccactgcgtc
     1021 gcaaatccta cgaaaaacca cccagcaaag ttcgctgcag ttacgccgaa ataaaaccct
     1081 tcgtgaggat aaaaatagat ctgccctaaa aaccatttgt gtccctgcaa cgcgacggga
     1141 tcaataacaa catcaagaag tgtcatgagt accccgccaa accacggagt cactcgggct
     1201 gtcaggcctt tatttaggtc gcgaccattg gcaacaataa aacccgccaa caaccaacta
     1261 aaataagaca aaaatacgaa tgagagtgaa tcccagaagg gcacgtttga gatccacagt
     1321 tcttgatttc gagtggtgtc aatgtaggta taaattccga aaggaaatcc attgcgtgta
     1381 gaactgtatt cacaaccaaa cgcgatcaaa taggtactcg cggtgaaaat cagcgatcga
     1441 aaaacaccca tgtgccgtat cgaaaaaact aaataacaaa gaaaaaatag gtaaacatag
     1501 ggtcgaagaa cgacgctgcc ccagaacaac ctcataactt ctaacacttg ctcaagcatt
     1561 cgacgccctt tgaagaaatc ttccgatagc catcattggg aaaaactggc ggtagagatg
     1621 ataccgcaag tagaaatggt taggaaaacc ggtaccagta aactcttcct cgtcccaatt
     1681 gccgttagat ttttgacgag taattaacca ttctatccct tttcttatag actcgtcagt
     1741 ggctggcaaa taagcgagaa gccccatcac tgcccaagca gtttgcgaag ccgtcgactg
     1801 gcccttaccc atcagcgcct tattagaata ggattctaga cattcgcccc atcccccgtc
     1861 ggcattttgt actgacttca gccagtcgag ggcacgaaca atcattggat cattagctcg
     1921 tacatttttt aaacgcgaaa gggcacaaag aacattcgac gttccgtata cataattcac
     1981 gccccaacgc ccgaaccaag acccttcttt ctcttgcgtc tttcgcaaat attcgacgcc
     2041 acgtttaata tttttttcga actttcgtcc gcctagaata cctagtgcct ccagcactcg
     2101 gcccgtaacg tccggggaac tggggtcgca gagcgagtcc atgtcggaaa aaggaatttc
     2161 attcagaaat attttatcat tattgatatc gaatgcagcc cagccgccgt cgcgattctg
     2221 cattccgacc gtccattcga ctgcccgttg tacagtaagc gattttgaaa aatcgcgatc
     2281 ctgctttagt agtccaataa tcacggccgc agtgtcatct acatcggggt accaggaatt
     2341 ttcatactca aatgcccacc cccccgaacg caatttcggg ttgtagacct tccaatctcc
     2401 gtaatcgacc aaaagttgtc tttgtttgat ccactcgcgg gcgcgtaaaa gtttttcatc
     2461 ttttccatcg tggccacaat caatcattcc gatcattgta agaacagtgt cccacactgg
     2521 tgacacgcag gcttgaactc gaaacccttg ttcgtcttcg attgcaaaac gttcgatagc
     2581 ctcgagcccg cccttaactt ctttcgaatc gagtgaatag ccgtgtaaat aaagcgctag
     2641 cactccattg tacatcgggg gaaatattcc agcccaatcg cctgtttttt cttggcgctc
     2701 caggacccat tcttcacatt tttttatagc gaatgccctt aaccatcgcg gtttgtaacg
     2761 ctcataggtt ctcaaaaata catcagaagc cgaaaagaag cttttccacc cggttccgtg
     2821 tttacgaagt atttcgacca aaggatcggt gtagggaata ttcttttgag tcggatttcg
     2881 ccacaaatga tcgagccaat cgttagaggc ggaccgacca ttgggaagtg caaaaatcgg
     2941 tttatgatga aaaatcataa acaaaggaac catagttcca cgtgcccaac tagagagcga
     3001 ataaatattc accggacttt tgggcggcaa aaaaataaat tcagggggta tcacaggaat
     3061 ggcagaccac ggccacagtc caaacatagc aaaattaatg cgggtaaaaa tacgaacacg
     3121 ctcgagcccg ccttgagcca atatatagtg ttcggctttt tttaggcgtt cgtcatttgt
     3181 cgatatcccc aacaaacgca gcgccaagta acattcggct gtggtggaaa ggtctcctgg
     3241 atgatttgag gcaatgcccc aacttccatc tgctttttga tttccaaaaa aatatcgaca
     3301 gatagcattt ttcttttttg aaagatccaa acctaacgcg tgctgtagga atacatattc
     3361 cgaagtaatc gtagcgtttg attcaagttc cgcgcaccag tgcccgtcct tactctgaat
     3421 actaatgcaa tagtctattg ctttttttgc ggctt
//
LOCUS       DAOMXO010000200         3433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15247_length_3433_cov_4.952635, whole genome shotgun sequence.
ACCESSION   DAOMXO010000200 DAOMXO010000000
VERSION     DAOMXO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15247_length_3433_cov_4.952635"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLH57_05235"
     CDS             <1..483
                     /locus_tag="PLH57_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02048.1"
                     /translation="KDFRKLVSTKSRFNRYFKQIEKALAARFGSSISLKRTFKLTAHS
                     GGYIPAATVLTHSFSRVESAALFDALYRNKESLVFARFARAKPFFSIYRKNTRTERYN
                     LGLLKRLQKNRHPSLLQAELTHVFSGCSASAVIAWTDDDHYDLVRLWYSSVLEWFTTL
                     "
     gene            480..1865
                     /locus_tag="PLH57_05240"
     CDS             480..1865
                     /locus_tag="PLH57_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRK02049.1"
                     /translation="MIRKTKASSHRNKFWITGAVLVTQNSQRDVLLADIVVDEHGVII
                     DLPKPRKSSPPRGFKKISAKGLHVLPGFVQTHVHLCQTLFRNTADDLELLDWLRKKIW
                     VYEARHNKFTLRTSALLGIQELLRSGTTCILDMGTVRYTEEIFKAAVETGIRANIGKC
                     LMDHPQNNPPELCESTEAALKEAHALFTKWNGTHGDRIRASYAPRFAISCTETLLQSV
                     AKSARERGAIIHTHASENRTECDLVMRMSGGRSNIEYLSATGICCERAVIAHGVWLSP
                     EEMRILSEKKASITHCPSSNLKLASGVAPVPELLEGGVNVALGADGAPCNNTLDMFHE
                     MRLAATLHKPRRGPTTLPAPEILDLATRNGAKALHWFDQIGSIEIGKKADFVALDLND
                     IATLPTHPTSLKAVASAIVYAGTASNVAWTMVDGQMCYRRSLSDQSTSARLLKDLQKA
                     RNAILRPVSPL"
     gene            complement(1925..3022)
                     /locus_tag="PLH57_05245"
     CDS             complement(1925..3022)
                     /locus_tag="PLH57_05245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK02050.1"
                     /translation="MERNIQVFLQIMFACTAVGLLPSLLRATESKQVTKCSAVLDDMK
                     LNSIQCPTCKKEPNVQLAVAKDYEYQSSDRSFEYSACEGCGSVMLTSPISRGELGNAY
                     PENYYSYHLLKNYDAYKNSVEGKRYFAEWRDAVAKDLTEEFLEKIGTTKAKARILDVG
                     GGDGLFLEILTQVGFQEKNLFLFDELAPVKHSTQTFDPRTASPGQFDIIFANQVVEHM
                     EAPEALFELAEKHLKKGGVLVLEAPSPTGIHFQLTKSGVWGGYHAPRHLYLLTPDSLQ
                     KLGEQNGLNLIESKYADDSWIWLQTLNNISKKYPATQTVLGKLSPAFATGIKEEPSRL
                     RRQVLAAFNRVNRVLKKIKSPTSAVKMVFKK"
BASE COUNT          884 a          837 c          768 g          944 t
ORIGIN      
        1 aaagattttc gaaaacttgt gtccacaaag agccggttca atcgctattt taagcaaatc
       61 gaaaaagccc ttgctgcacg gtttggatct tcaatctctt taaaaagaac gttcaagctc
      121 actgcacaca gcggggggta cattccagcg gctacggtct tgacccactc tttttctcgt
      181 gtcgagagcg ccgcactttt tgacgctctt taccgaaata aggagtcttt agttttcgcg
      241 cggttcgcta gagcgaaacc ttttttttcg atttaccgaa aaaacacgcg aacggaacgc
      301 tacaatcttg ggttacttaa gcgccttcag aaaaatagac atccgtcgct tcttcaagca
      361 gagctaaccc atgttttttc ggggtgtagc gcaagcgccg tgatcgcttg gaccgatgac
      421 gaccactacg atctcgttcg actctggtac agttctgtgt tggaatggtt cacaacgcta
      481 tgatcagaaa aacgaaagcc agctctcatc gaaataaatt ttggatcacc ggtgcggtgt
      541 tagtgactca gaacagccaa cgcgatgttc tgctcgcaga tatcgtagta gacgaacacg
      601 gtgtgatcat cgaccttcca aaaccccgca aaagttcgcc acctcgtggt ttcaaaaaaa
      661 tttcagctaa agggctccac gtgcttccgg gctttgtgca gacccacgtg catctttgcc
      721 aaacgctttt tcgtaacact gcagatgatc tcgaacttct cgattggttg cgaaaaaaga
      781 tttgggtcta cgaggctcgc cacaataagt tcacgctacg caccagtgca cttcttggaa
      841 ttcaggaact tttgcggtcc ggtacaactt gtattttaga tatgggcacg gtccgctata
      901 cggaagaaat ttttaaggcc gcagtagaaa ctggaattcg cgccaatatc ggcaaatgtt
      961 taatggatca tccacaaaat aatcctcctg agctatgcga atcgaccgaa gccgcattga
     1021 aagaagccca cgcgctcttt accaagtgga acgggactca cggcgatcgc attcgcgcga
     1081 gttatgcccc aagatttgcc atttcctgca ccgaaacact tttacaaagc gtggcgaagt
     1141 ctgcacgcga gcgtggagcg attattcata cccacgcatc tgaaaaccga actgaatgcg
     1201 atctagtaat gcgaatgtcg ggagggcgtt ctaacattga atatttaagt gcaactggga
     1261 tttgttgtga acgcgctgtt attgcccacg gtgtgtggct tagccctgag gaaatgcgaa
     1321 tcttgagtga aaagaaagcg tcaatcaccc actgtcctag ttctaatctt aaactggcaa
     1381 gcggtgtcgc gcccgtgccc gaactgctag agggcggcgt gaacgtagcg ctcggcgctg
     1441 acggggcacc gtgtaacaac accctcgaca tgtttcacga aatgcggctt gctgctacgt
     1501 tacacaagcc acggcgtggc cccacaacgt tgccggcgcc tgaaattcta gatctcgcaa
     1561 ctcgcaacgg tgccaaggcg cttcactggt ttgatcagat tgggtctata gaaatcggaa
     1621 aaaaggccga cttcgtggct ttagatttaa acgacatcgc cactttgccg acgcatccga
     1681 cttcattaaa ggccgtggcg tcagccatcg tctatgctgg gacggctagc aacgttgctt
     1741 ggactatggt cgacgggcaa atgtgttatc ggcggtcgct aagcgaccag agcacatcgg
     1801 cgcgtttatt aaaggacctt caaaaagctc gaaacgcgat tctgcgccct gtatccccgt
     1861 tataaataga atcttttctt ttagggagct gttccgcatg ttcacactca cggtggtagc
     1921 cgtttcattt tttaaatacc atttttactg cagaggtcgg ggactttatt ttcttcaaaa
     1981 cccgattcac tctattgaaa gctgctagaa cctgtctacg taaccggctt ggctcttctt
     2041 ttattccagt cgcaaaggcc gggcttaatt tacccaatac ggtttgggtg gcgggatatt
     2101 tttttgaaat attatttaat gtttgcagcc aaatccaact atcgtctgca tatttgctct
     2161 caattaagtt gagtccattt tgttcgccaa gcttttgcaa agaatccgga gtgagtaagt
     2221 acaaatgtct gggagcgtga taccctcccc acactccaga tttagttagt tggaagtgaa
     2281 ttcccgttgg agaaggcgcc tctaacacga gtactcctcc tttttttaaa tgcttttccg
     2341 cgagctcaaa tagggcctcg ggggcttcca tatgctcaac tacctgattg gcgaatatta
     2401 tatcaaattg ccctggagaa gctgtcctcg gatcaaacgt ttgagtcgaa tgttttaccg
     2461 gcgctaattc gtcaaataaa aatagatttt tctcctgaaa tccgacttga gtaaggattt
     2521 ctaggaacaa accatcgccg ccacctacat ccaggatcct agccttagcc ttggtggtcc
     2581 ctattttttc caaaaactcc tcagtgagat ctttagcgac tgcatcgcgc cattcggcga
     2641 aatacctttt accttcgacc gaatttttat atgcgtcata gtttttaaga agatgatacg
     2701 agtaatagtt ttctggatag gcattgccaa gctcgcctct tgatatgggt gaggtaagca
     2761 tcacgcttcc gcaaccttcg caggcactat attcaaacga tcgatcacta gactggtact
     2821 cgtagtcttt tgccactgct aactgaacat tcggttcctt cttacaggtt ggacattgga
     2881 ttgaatttaa tttcatatca tctagtacgg ccgaacattt ggtaacttgt ttagattcgg
     2941 ttgcgcgcaa tagcgacggc aacaaaccaa ccgcagtaca cgcaaacatt atctgcaaaa
     3001 atacctgtat attcctttcc atagcattgg gtcgaatttc atacaatttt tgtcggatat
     3061 aatcaagcaa ttttagatac cgacagttaa tcattcaatt acgactaact tctgacgaag
     3121 agggccattc aagggctgct ccgcagataa acgcgatcga ccaacccact tgaactctga
     3181 atcccgtaac cgagaacatt ctgcccctta ttcatgcggc gttcttgctc gaaacgaatt
     3241 cgtcttttac aatttgcggt atgaacacat gtggatacgg acctcaatgt tgggttagtt
     3301 gcattctcat ctgccttggc tggttgcttg ctagctctgg cttgctctac ttcacttgga
     3361 acaaggtgat cgccgaggtc accaaaatga aacaagtgaa atattggcaa gcactcttgt
     3421 ttctagccac agt
//
LOCUS       DAOMXO010000201         3413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15384_length_3413_cov_4.510423, whole genome shotgun sequence.
ACCESSION   DAOMXO010000201 DAOMXO010000000
VERSION     DAOMXO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15384_length_3413_cov_4.510423"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLH57_05250"
     CDS             complement(<1..890)
                     /locus_tag="PLH57_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HRK02051.1"
                     /translation="MKRRLGGKQNESGIALFMVISTVALLGVLVTELTYSTQVSSRLA
                     YNALDNAKAYYLAKAAYRFSLVRLSAYAAVKKFLDDPQNKTIKATVNPFVDRIWDIPF
                     AFPPPLPKDAPASVADPVKEFVAESSLAGNYTAMIFGQSSKINFNNLFIKEVDAETES
                     ENSNTGNSGASNNAKSSNKTTNSNGEEKEEVNFRPMVETTISTLLNQKMSQDQEFAEV
                     YRNVTGADVIDAIEGYLKRESRGSALPGYRAPETPKEAPFYDISELHMVPGINDEIYN
                     LLIDTFTASATPGINVNMASR"
     gene            complement(887..1645)
                     /locus_tag="PLH57_05255"
     CDS             complement(887..1645)
                     /locus_tag="PLH57_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRK02052.1"
                     /translation="MKGFTLLELLISIMILSFISLGVYQTTSSSFSLREDLEKDGDFY
                     NSIRVALDIIGRDIAHMYSPQGSAFPGDIGKATIIDPAKPNEPKERSPYTDVIWPFWG
                     GAINAMGVRPARFLGEATKMTFIANSNFRLFRDAPESDFIKVTYSYEEDTLAGRGTRA
                     LFRRTHTDVFTEREDSATEVRYLLISRLKSLKFSFLDGEKDRWDMTPRWDTAEGLYKD
                     VYPAMIRVEIEVFTPNSDENTFSISQLYRPELNL"
     gene            complement(1642..2145)
                     /locus_tag="PLH57_05260"
     CDS             complement(1642..2145)
                     /locus_tag="PLH57_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRK02053.1"
                     /translation="MISARPRSQQGFTLLEVMIAVAIMVVAFTAILTSQSSAIQLVIK
                     SKEINIATWLAKNKMQESEHLFEGKPFDELEKEKEETFPEPFAQYKWHREIKEIKFPE
                     ITLPAKEEGGVPEPLRIMAQTLTRHFDKTIREMVVTVKWQRGTSEQKIVISTYLVDLN
                     KEFDFSI"
     gene            complement(2142..2780)
                     /locus_tag="PLH57_05265"
     CDS             complement(2142..2780)
                     /locus_tag="PLH57_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRK02054.1"
                     /translation="MQPTRITKKQECQRGFTLLEILIVIALVAMVSAIAIPSLGVGLK
                     VNIDAASRNLASIVRTTRDEAVLKGQIYRVAFDIEKGQYWAEIGDRSFLMRTSEQEEE
                     ERRRKERYGTADEKDNEKAQRRDDGFSMAASIKKKKQSLPTGIKFVDLINSRTKEPQT
                     SGIVYAHVFPHGFVEKLIIHVKDSLDRQATLVVDSVSGKSRVFNRYVKESEI"
     gene            complement(2805..3260)
                     /gene="gspG"
                     /locus_tag="PLH57_05270"
     CDS             complement(2805..3260)
                     /gene="gspG"
                     /locus_tag="PLH57_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRK02055.1"
                     /translation="MKIKQKNKGVLQAGFTLVEIMIVITLIGIVATLFVQNYTGKLEE
                     GKRKSTKILMQQIRTALDDYYRNCNGYPTTAQGGLDALVKAPADGSCKDYDPNGYLKD
                     RRVPKDAWDNDFIFLGDDGRTYVLKSLGADRKEGGEGNSKDISTDDPEF"
BASE COUNT          867 a          817 c          727 g         1002 t
ORIGIN      
        1 cgcgaggcca tgtttacatt gattccgggc gtagcactgg cggtaaaggt atcaatgagc
       61 agattataaa tttcgtcatt gattccaggc accatatgaa gttctgaaat gtcgtaaaat
      121 ggcgcctctt tcggagtctc gggtgctcgg taaccgggca atgccgaacc gcgagactca
      181 cgcttcaagt aaccttcaat cgcatcgatt acgtctgcac cggtgacatt tcggtaaacc
      241 tcggcaaact cctgatcttg actcattttt tgatttagaa gcgtggaaat cgtggtctcg
      301 accatcggac gaaagttcac ttcttctttt tcttcaccat tgctatttgt ggttttgtta
      361 gagctttttg cattatttga ggcgcccgaa ttaccggtgt tactattttc agattctgtt
      421 tcggcatcca cttctttaat aaaaagatta ttaaaattta tctttgaact ctgaccgaaa
      481 atcatagccg tatagttccc cgccagcgaa gactccgcca cgaattcctt taccgggtcg
      541 gcaacgctgg ctggggcgtc tttcggaagc ggcggcggaa aagcaaatgg gatatcccaa
      601 attcgatcca caaacgggtt tacagttgct ttaattgttt tgttttgtgg gtcatctaaa
      661 aatttcttaa cggcggcata agcgctcaaa cgtaccagtg aaaatctata cgcagcctta
      721 gcaagataat aggctttggc attatcgagc gcgttgtagg ccaatctcga actcacctga
      781 gtcgagtatg tgagttcggt cactaaaacc cctaatagcg ctactgtgct aattaccata
      841 aacaaagcga tgccagattc attttgtttt ccacccaatc ttcgtttcat agattgagct
      901 ccgggcggta aagttgagaa atactaaagg tattttcatc ggaattagga gtaaacactt
      961 caatctctac tcgaatcatt gcggggtaaa catctttgta gagcccttcc gcggtatccc
     1021 aacgaggggt catatcccac cggtcttttt ccccatcgag aaatgaaaac ttaagcgact
     1081 tcagtctcga aattagcaaa tatcgtacct cggtagcgga gtcttcacgt tcggtaaata
     1141 cgtcggtgtg ggtacgccta aatagagcac gcgtacctcg ccccgcgagc gtgtcttctt
     1201 cataggagta ggtgacctta ataaagtcac tctccggagc gtcgcgaaat aggcgaaaat
     1261 tggaatttgc gatgaaagtc attttggtgg cttcacctaa aaaccttgca ggtcgcactc
     1321 ccattgcatt gattgcgccg ccccaaaatg gccaaatcac atcggtgtaa ggagagcgtt
     1381 ctttaggttc atttggctta gccgggtcaa taatggtcgc cttgccgata tcccccggaa
     1441 aagccgagcc ctgcggagaa tacatgtgtg caatatcgcg acctataata tccaatgcga
     1501 ctcgaataga attatagaag tccccatctt tttcgagatc ttcgcgcaat gaaaaactcg
     1561 acgaggtcgt ttgatatacg cccaaactaa tgaacgacaa aatcatgatg gaaatcaata
     1621 gttcaagaag cgtgaatccc ttcatatcga gaaatcaaac tccttattta aatcaactaa
     1681 ataggttgaa atcactattt tttgctcaga agtgccgcgt tgccacttca cagtcactac
     1741 catttccctg attgttttat cgaaatgtcg agtaagcgtt tgagccatga tacgcaatgg
     1801 ctcgggcaca ccaccttctt cttttgctgg tagcgtgatc tctggaaact ttatttcctt
     1861 tatttcccga tgccacttat attgggcaaa aggttccgga aaggtttcct ctttttcttt
     1921 ttcaagttcg tcgaatggtt tcccttcgaa taaatgttcc gactcctgca ttttattttt
     1981 agcaagccag gtggcgatat tgatttcttt actcttaata acaagctgaa tcgccgaact
     2041 ttgcgaagtt aaaatcgcgg taaatgcgac taccatgatc gccactgcga tcatgacctc
     2101 gagcaacgtg aaaccttgtt gggaccttgg ccgcgcggag atcatatttc gctctcctta
     2161 acgtaccgat taaatactcg tgacttacca ctcaccgaat cgaccacaag cgtggcttgg
     2221 cgatctaaag aatctttgac gtgaataatt agtttttcaa caaaaccgtg gggaaataca
     2281 tgtgcataca caattccgct cgtttgcggc tctttggttc tagagttgat gagatcaaca
     2341 aatttaatac ccgtgggaag gctttgcttt tttttcttaa tggaagctgc catcgaaaaa
     2401 ccgtcatctc gcctttgagc cttttcgtta tctttttcgt cagcagtccc gtaacgctct
     2461 ttacgtcgcc gttcctcttc ttcctgttcg ctagtgcgca ttaaaaagga acgatctcct
     2521 atttctgccc agtactggcc cttttcaata tcgaatgcga cgcgatagat ctgtcctttt
     2581 aggaccgcct catctcgcgt ggtgcgcacg atagaagcga gatttcggga cgcggcgtcg
     2641 atatttactt tcagtcctac gcctagagag ggaattgcga tcgcagagac catcgcaacc
     2701 aacgcgatca caataagaat ttcaagcagg gtaaaacccc gctgacattc ttgtttctta
     2761 gtgattcgcg ttggttgcac gatggtattc cctcatattt tcaactagaa ctcggggtcg
     2821 tcagtactta tgtctttaga gttcccttcg ccgccttctt tacggtcagc tccaagagat
     2881 ttcaaaacgt acgtacgacc atcatcaccg agaaaaataa aatcgttatc ccaggcgtct
     2941 ttcggtacgc gacggtcttt taaatagccg tttggatcat aatccttaca cgatccgtcg
     3001 gccggagcct tcacgagtgc atcgagtccg ccctgagcgg tggtgggata tccgttacag
     3061 tttcgatagt aatcatcgag ggcagtacga atctgttgca tcaaaatttt ggtgctcttg
     3121 cgtttacctt cctcgagttt acctgtgtaa ttttgcacaa aaagtgtcgc tacgattccg
     3181 attaacgtga tcacgatcat gatttctaca agcgtgaacc ctgcctgcag aacgcctttg
     3241 tttttctgtt ttattttcat actacgttct cctttgttgt ttttacgaga tgttattgag
     3301 ttctagtaac ggcacaaata tcgccgccac gatcccgcca actacaagtc ccatgaaaat
     3361 gatcatcgcg ggttccaaca atgaagtgag accctcgatt ttcactgaaa cct
//
LOCUS       DAOMXO010000202         3399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15475_length_3399_cov_4.830443, whole genome shotgun sequence.
ACCESSION   DAOMXO010000202 DAOMXO010000000
VERSION     DAOMXO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15475_length_3399_cov_4.830443"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLH57_05275"
     CDS             complement(<1..444)
                     /locus_tag="PLH57_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02056.1"
                     /translation="MQRRTLLIAWVSVFLLVSCAHKKEVHPPVAQVVPNDTNGASLAL
                     TVEKKADSARPEISDRDEKRVDDMPAVESETALARPVAAPSPDTQEPVRRSDYHKLTD
                     IKKIWISLAFANVRVQTGASKKNSEARVKVFPPQGHTLKTDHCSAH"
     gene            complement(566..1099)
                     /locus_tag="PLH57_05280"
     CDS             complement(566..1099)
                     /locus_tag="PLH57_05280"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRK02057.1"
                     /translation="MVKIYQKAPEFSTQGYYEGKISNFSLKDWQGKWVCLFFYPLDFT
                     FVCPTELQQFTEKSEAFKRLNCQVASVSVDSPYSHEAWSKGPLKEQRYPMLSDLNHAI
                     SQSYGCYLEDKGIALRATYIIDPQGTLQWAQYHPLNVGREADEILRVLTALQTGELCQ
                     AGWKNGEETLTKKLARK"
     gene            complement(1212..>3399)
                     /locus_tag="PLH57_05285"
     CDS             complement(1212..>3399)
                     /locus_tag="PLH57_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02058.1"
                     /translation="GASADMLVSTLSRLLGWTLDAPVIKHVAQAFSQTGTYLHEPWAA
                     TRLMVGIGAVLALRPLTLFLAHQYSKIKKTGWSPVKALFTYGTRIYASLLWVGYGPQK
                     LVIEKIMRQKNVYRAVDLGMNPLTTRAAWHAPWASKSKVEEARQKLEKTANADTLVRR
                     ARALTLAATIVSEGRGIDVPTLLMATEAAEQNALRPFLETLGSLPAEVTWRELSSKVY
                     AALNDLHDRGVGVVTAETPKEELEAYVESMRNVADEIDRLVTDRKTGVDKKSAVRAAR
                     WTKEKAKTWKRLTTDAVLPFFFIGSAAYKERKKYTGFESSEQGGKIGYMQYKEDYDFS
                     AWFYAGTDPRLFGGAAGGNVVDIARVSGDQVEQNLLYGLLNGYDVESSLAADANALTN
                     VFEVLNNDKLILKRIREQTTGEAAEVIVNDTADPNGDSYFAVHQRTLNANLTGFHLRT
                     VVGVIPKTLSLAAIAIAANPGSFATALGAGFAGAIQSVPRQAVIILKKYGISPDGDVG
                     YATVWPGVRNASRALEKNVKANLKKIQSIDGKMASAEEANMRAATRELLDLYRASGTP
                     IPQEFAVSPAEYTPEKMKQLWTYSLSSPEAVPAPTKASHFMQAKLLNASYGALISTVI
                     FLAVSKNFYNPKESVWTQLVEGLVTMAVTYVGVSVMKEVAPKIAAVPAVNWTAKKFGA
                     GLRTGFNVLGTVWRGVTWAPRKVKAAVVYAAVSCREMLSSKDKKSK"
BASE COUNT          807 a          909 c          782 g          901 t
ORIGIN      
        1 atgggcgcta cagtggtcag tcttgagcgt gtgtccctga gggggaaaca ctttaactcg
       61 cgcttcactg ttctttttgc tagcgccggt ttgaacacgc acgttcgcaa atgcgagtga
      121 gatccagatt ttttttatat cggtaagttt gtgataatcg gaacgccgca ccggttcttg
      181 ggtatcgggg ctgggcgcag caacggggcg cgctaacgcg gtttccgatt ctactgccgg
      241 catatcgtcc actcgttttt cgtctcggtc actgatctca ggacgagcgg agtccgcttt
      301 tttttccacc gtcagtgcca ggctcgctcc gttggtgtcg ttcggcacga cttgcgcaac
      361 cggcgggtgc acttcctttt tatgagcaca ggaaacgagt aaaaaaacgg aaacccaagc
      421 gattaagagt gttcggcgtt gcatgcacct actagcatac tgatagagtt ggtttttttt
      481 caagcaaaca aaaaatgaga ggagaaagag ggttagaggg acgaaaatca cgcaagtgac
      541 tttcgccccc taatgtgaac cgaaactact tgcgagccag tttcttggta agcgtttctt
      601 cgccgttttt ccagccagct tggcagagtt cgccggtttg aagtgcggtg agaacgcgca
      661 agatttcgtc ggcttcacgg ccaacgttga gcgggtggta ttgagcccac tgcaaggtgc
      721 cttgcggatc gataatgtag gtcgcgcgca aagcaatacc cttgtcttca aggtagcagc
      781 cgtagctttg actgatcgca tgattcaggt ccgaaagcat cggatagcgt tgttccttga
      841 gcgggccttt cgaccaagct tcgtgggaat aggggctatc gacgctcacg ctggccacct
      901 gacaattcaa gcgtttgaat gcctctgatt tttcagtaaa ctgctgcaac tcggttgggc
      961 aaacaaacgt aaagtcgagc ggatagaaaa acaaacacac ccacttgcct tgccaatctt
     1021 tcagcgaaaa attggagatc ttgccttcgt aatacccctg agtcgaaaac tcaggcgctt
     1081 tttgatagat ttttaccatg tgaaacgtct cctgtttagt cagccctttg ggggctagtt
     1141 cattaatgaa ccttgtaccc accgtgtatc agactgaata aacagtgcca attccaaaga
     1201 acccagtctt tttatttcga cttcttatct ttagaagaca gcatttctcg acaactcact
     1261 gcagcgtaaa ccaccgccgc tttcactttg cgaggagccc aagttacgcc tcgccagacg
     1321 gtccccaaaa cattgaaccc tgttcgtaaa ccggctccaa actttttggc tgtccaattc
     1381 accgctggaa ccgctgcgat cttcggcgca acttctttca tgaccgaaac accaacgtaa
     1441 gttactgcca tagtaacgag tccttctact agctgcgtcc agaccgactc tttggggtta
     1501 taaaagtttt ttgaaaccgc taaaaaaatc acggtactta tcagagctcc gtacgaggcg
     1561 tttagaagtt ttgcctgcat gaaatgactc gccttagtgg gcgcaggtac cgcttccggt
     1621 gaagacaaag aatacgtcca aagttgtttc atcttctctg gcgtatactc ggcggggctc
     1681 actgcgaatt cctgcggaat cggtgtaccc gacgctctgt atagatccaa aagctcccta
     1741 gtagccgctc gcatgtttgc ttcttctgct gaggccatct tcccatcgat gctctgaatt
     1801 tttttaaggt tggcttttac gtttttctcc agcgcacgag acgcattacg cacacccggc
     1861 caaaccgtcg catacccaac atccccatct gggctgattc catatttttt tagaataatt
     1921 acagcctgtc gagggacgct ttgaatagct cccgcgaaac cagcacccaa agcggttgca
     1981 aaactaccgg ggttcgccgc aattgcgatc gcagccaaac tcaatgtttt tggaattact
     2041 cctacaaccg ttcgaaggtg aaatcccgta agattcgcat tcaaagtacg ctgatgtacc
     2101 gcaaaatagc tgtcgccatt cggatcggcc gtgtcgttaa caatcacttc agccgcctcg
     2161 ccagtcgtct gttctcgaat gcgctttaaa atcagcttat cgttgttcaa cacttcaaac
     2221 acgttggtca acgcattggc atctgccgca agcgaagatt caacgtcgta accgttcaaa
     2281 agaccataca aaagattttg ttcaacctgg tctccgctta cccgtgcgat atcgacaacg
     2341 tttccgccgg cggctccgcc gaagagtcgc gggtctgttc cagcgtagaa ccaagcacta
     2401 aaatcgtaat cctctttata ctgcatgtag ccgatttttc cgccttgttc acttgattca
     2461 aacccggtgt actttttacg ttctttgtac gcagctgaac cgataaagaa aaacggtaac
     2521 actgcatccg tcgtaaggcg tttccatgtc tttgcttttt cttttgtcca cctagctgca
     2581 cgcacagcgc tctttttatc gacgcctgtt ttacggtcag tcactaagcg atctatctcg
     2641 tcagcaacat tgcgcataga ctccacataa gcttcaagct cttctttcgg agtttccgcc
     2701 gtaaccactc ccactccacg atcgtgcaga tcattcaacg ctgcatagac tttcgaagac
     2761 agctctctcc acgtcacttc cgccggaagt gaacctaaag tttctagaaa cggcctcaaa
     2821 gcattttgtt cggctgcttc ggtggccata agcaaagttg gtacgtcaat tccacgtcct
     2881 tccgaaacaa tagtcgcggc caatgtaaga gcccgagctc gtcgaacgag ggtgtccgcg
     2941 tttgctgttt tttcaagttt ttgcctagct tcttcaacct tagatttcga agcccatggc
     3001 gcgtgccacg ctgccctcgt ggtcagagga ttcatcccaa ggtctaccgc acgataaaca
     3061 tttttctgtc tcatgatttt ttcaatcacg agtttttggg gtccgtaacc tacccacaac
     3121 agagacgcat agatacgagt cccgtaagta aagagcgctt tcaccgggga ccatccggtc
     3181 ttcttaattt tggaatactg gtgagccaaa aataaagtca gcggtcgcag cgccaacacg
     3241 gctccaattc cgaccatcaa tcgggttgct gcccagggtt cgtgcaaata agttcctgtt
     3301 tgcgaaaagg cctgcgccac atgtttaatc acgggtgcat cgagcgtcca accaagtagg
     3361 cgcgataacg tcgacacgag catgtcggcg ctcgctccg
//
LOCUS       DAOMXO010000203         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15541_length_3388_cov_4.989199, whole genome shotgun sequence.
ACCESSION   DAOMXO010000203 DAOMXO010000000
VERSION     DAOMXO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15541_length_3388_cov_4.989199"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PLH57_05290"
     CDS             complement(<1..825)
                     /locus_tag="PLH57_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRK02059.1"
                     /translation="MPVGKRPSQEVDLHETGGATERRALDVALAREASIQVVPTPLKN
                     ATLSLNGLAGEHVAIATDGDPVVGRVEGAFDARGLPLGSAERVAIYQGLNSLPFGSQN
                     IAGVINVESPWSVEPNTDLSWAMGSLGRMLIRASTQRSSAWTSFSNWRSEAVVHPERP
                     QNTLSNAVTRTGVDAGAVKKNVFTSRGRSFDARVTGLFIDQNQKGLFGGERTLQDQNR
                     YGAAIDLKSGDSSFRVSWNRFESKFQGLATDDSPNRQDRESEQLWRFGPQIEENISG"
     gene            1183..>3388
                     /locus_tag="PLH57_05295"
     CDS             1183..>3388
                     /locus_tag="PLH57_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02060.1"
                     /translation="MNSAFKLNHPRVFRQLLTAAFTALALHATPLFAITGSNTSPSPT
                     GSPLLQQRPAPSLKPLTEVTKDQSAFSACFLNFRKPNAETTKIKAALKKLNFNIISIN
                     SDSLKYGSFDKLCTDIKNQLSSEKRISTAPTCDVLVIDADFDIRFHNALNAPERHATA
                     GMYELLYGKCSGNCPTLLSNVSQVFVVGANTPLTIARPIAKKAFSDNLEKRGYPPDMI
                     ESLWSYRLQEISKHKAGQITSATIFQNAFGPTVDLFAVDLGIIDPSSSRQELQLERTL
                     YSAEIRTLKKRIASKRKIRFSEKLFEFFHLTKPEKPAKKISWEKTKLEALIGQMNNPG
                     FLYCEQDFSTTKDAQSACEQFFNDNRLNSSSLKIFDDPLNLEEEIERHRYTRQFGKLN
                     FRRDERLRELKIENRKLPRSMNIYAAMAYSTDFTHSGYVFDRDFWVKHFELNHIKERL
                     TGAPPSLKEADKLCQEFSNISYHLLPSNTAINPEWYQSIGGVLLLTCILDVGPYWASR
                     ADILNSPSDDDQITFLAHTIDTSAEATSSLPRSLKLVEKTGCTAYRFIQNGLASKNPD
                     FHAIATKAAVTLYTTISTCSRAGTEHQALVTAFKDILKTTDIGSWSLKEHAIDVYLDQ
                     LWASNSLKFIWSDLYDTLTHPENQRFATLERALRLGSWLTDANDLTRLVDDFKAAEPG
                     GSEWRFLAATLQSAAQHNRISEKQKKSLNLNSSTFKKMEKYLDNSIDLFLLAC"
BASE COUNT          938 a          910 c          742 g          798 t
ORIGIN      
        1 gcccgaaata ttttcttcta tttgtggtcc gaagcgccag agttgctctg actcccggtc
       61 ttgtcgatta ggagaatcgt cggtggcgag tccctgaaat ttactttcaa aacgattcca
      121 ggaaacacgg aacgatgaat cgccactttt taaatcgatt gcggcgccgt agcgattttg
      181 atcttgcagt gtacgttcgc cgccaaatag gcctttttga ttctggtcaa taaacaaacc
      241 cgttacgcga gcatcaaaag atcgccccct ggaggtgaaa acattttttt tcacagcacc
      301 ggcgtcaacg cccgtgcggg tgacggcgtt ggagagtgtg ttttgcgggc gctcggggtg
      361 caccacggct tcggagcgcc agtttgaaaa agaagtccat gccgaactgc gctgggtcga
      421 ggcacgaatg agcatgcgtc caagcgagcc catcgcccag gaaagatctg tgttcggctc
      481 taccgaccat ggggattcga cgttaatgac acctgcaata ttttgagagc caaaaggcag
      541 ggagttcaac ccttggtaaa tggctacccg ttcggccgat ccgagcggca gaccgcgcgc
      601 atcgaaggca ccttcgactc ggcctacgac tggatcgccg tcggtggcga ttgcgacgtg
      661 ttcacctgca agaccgttta gtgaaagagt ggcgtttttc aagggagtgg gcaccacttg
      721 gatgctggct tctcgagcta acgctacgtc gagtgcgcgc cgctcggttg caccaccagt
      781 ttcgtgaagg tccacctctt gggagggccg ttttccaacg ggcatcctcg gtgccgagat
      841 ctcgacgtca ctcgcggtgg acaatgacga accgtgcgcg accgtggtgg accaagcccc
      901 aactgtccaa agcagcacac tgcaaattag gtaaaagtag cgttttcgca ccatattaaa
      961 ccggaatggt tgactaacac taccggtcaa taaaggcaac gcgggagggg cagggcaacc
     1021 cagtttcgaa aacaggcata cggaccgctt tttgattcca caacctggtc agtctcccca
     1081 aaaaacgacc caggctaaac taggaactca ttgccgacaa agagctatgg aggaccagct
     1141 aacgccttct atatctgagc caaaacaccg acaaatatat gaatgaactc ggcatttaaa
     1201 cttaaccatc ctcgggtgtt tcgtcagctg ctaaccgccg cattcactgc cctcgcattg
     1261 cacgcaactc cgttgtttgc cattactggt tcgaacacct caccttcgcc taccggctct
     1321 cctctactac aacaacgccc cgccccttcg ctaaaaccac taacggaagt tacgaaagat
     1381 cagtccgctt ttagtgcgtg ctttttaaac tttcgtaagc ctaacgccga gaccaccaaa
     1441 atcaaggccg cgctcaagaa attaaacttc aatattatct cgataaactc tgactccctt
     1501 aaatacggat cgttcgataa actttgtacc gatataaaaa atcaactgag ttccgaaaaa
     1561 cgaatttcta ctgcgccaac ttgcgacgtt ttagttatcg atgccgactt tgatatccgt
     1621 ttccacaatg ccctcaacgc gccagaacgg cacgctacgg caggaatgta cgaactcttg
     1681 tatggcaaat gctccggcaa ctgcccgacc ttgctctcca acgtcagtca agtcttcgtg
     1741 gttggtgcaa acacgccgct tacgattgct cgccccatag caaaaaaagc attttccgat
     1801 aatctcgaaa aacggggata cccacccgac atgatcgaat cactttggag ttaccgactc
     1861 caagaaatat caaaacacaa agccgggcag attacctccg ctaccatttt ccaaaatgcg
     1921 tttggaccga cggtcgattt atttgcggtg gatcttggga taatcgaccc atcgagttcg
     1981 cggcaggagc tccaactaga acgcacgctt tattctgccg aaattcgtac tctgaaaaaa
     2041 cgcatagcat cgaaacgtaa gattcgattt tcggaaaaac ttttcgagtt ctttcacctc
     2101 actaaacccg aaaaaccggc caaaaaaatt tcgtgggaaa aaaccaaact cgaggcgcta
     2161 atcggacaaa tgaataatcc tggttttctc tactgtgaac aggacttttc tacgacgaaa
     2221 gatgcacaat ctgcctgcga acaatttttt aacgataatc gcctaaacag tagtagttta
     2281 aaaattttcg atgacccctt aaatcttgaa gaagagatcg agagacaccg atacacccgt
     2341 cagttcggta aacttaactt tcgacgcgac gagcgactac gtgaattgaa aattgagaat
     2401 cgtaaactac cacgcagcat gaatatttac gctgccatgg cctactctac cgattttacc
     2461 cattcgggtt atgtcttcga ccgcgatttt tgggtaaaac attttgaatt aaaccacatt
     2521 aaagaacgct taaccggagc cccgccttca ttaaaagagg ccgataaact ctgccaggaa
     2581 ttttcaaaca tttcttacca cttactccct tctaatacgg caataaaccc cgaatggtat
     2641 cagagtatag gcggggtcct gctgttaaca tgcatcttag acgtcggccc ttactgggca
     2701 tctcgagcag atattcttaa ttcaccatcc gacgacgacc aaattacttt tctagcccat
     2761 actatagaca cctcggccga agcgaccagc agtctgcctc ggtctttgaa gctcgtcgaa
     2821 aaaactggat gcacagctta tcgattcatt cagaacggac tcgcaagtaa aaacccagac
     2881 tttcacgcca tcgccaccaa agcagccgtt acactctaca cgacgatttc cacatgcagt
     2941 cgcgccggaa cggaacacca ggctctagtc acagctttca aagacattct caaaacaact
     3001 gacattggat cgtggtcact aaaagaacac gcaatcgatg tttatctaga ccaactttgg
     3061 gccagcaaca gtcttaagtt catctggtcg gacctttacg acactctgac ccacccagaa
     3121 aatcaacgct tcgcaactct cgaacgagca ctcaggcttg gcagttggct gacagatgcc
     3181 aatgacctta cacgtttagt agacgacttt aaggcggcgg aaccgggcgg cagtgagtgg
     3241 cggttcctgg cggccactct ccagtccgcg gctcaacaca atcgaatttc ggaaaaacaa
     3301 aaaaaatctc taaacttgaa ttcgagtaca ttcaaaaaaa tggaaaaata tctagataac
     3361 tcgatcgatc tatttttgct cgcttgtc
//
LOCUS       DAOMXO010000204         3366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15670_length_3366_cov_4.618846, whole genome shotgun sequence.
ACCESSION   DAOMXO010000204 DAOMXO010000000
VERSION     DAOMXO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15670_length_3366_cov_4.618846"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLH57_05300"
     CDS             complement(<1..548)
                     /locus_tag="PLH57_05300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458574.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HRK02061.1"
                     /translation="MAEKIVSKYFYNKEAAALSFYQKDGGYSAAKKALGMAVDDVIAE
                     VKKSNLRGRGGAGFPTGMKWSFIPKDSKAPKYLVCNCDESEPGTFKDRAIITYSPHML
                     IEGMLIGAHAIGAHVGYIYIRGEYVREAEFLEHAIDEAYKAGILGKNAMGSGFKFDLY
                     VHRGAGAYICGEETGLLNSLEG"
     gene            complement(563..1036)
                     /gene="nuoE"
                     /locus_tag="PLH57_05305"
     CDS             complement(563..1036)
                     /gene="nuoE"
                     /locus_tag="PLH57_05305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRK02062.1"
                     /translation="MKKQLSDKFYKEIDRLRPRYPTNQALLLPALHLAQDELGWVSGD
                     TMDEIAAAIDIPPPVVREVITFYTMYNLKPVGNYHLQVCTNISCALMGAEKLLEHCEK
                     KLGIQAGETTRDNKFTITEVECLGACGTAPCVQINKDYYEDLTTEKMDRILSELK"
     gene            complement(1051..2811)
                     /gene="nuoD"
                     /locus_tag="PLH57_05310"
     CDS             complement(1051..2811)
                     /gene="nuoD"
                     /locus_tag="PLH57_05310"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HRK02063.1"
                     /translation="MAYFAEKTNATDQQLERIVAAQGAAILKTEKDRAGCPVIWVSKT
                     SLMVVLKFLKEADGLEFNFLADLTAYDDLGSGEDGSGRFVLVYNLFSLRNLNRIRIKL
                     RLKDQESAPTAVPLWAAANWAEREVFDLFGIKFDGHPDLRRILLDIRWEGHPLRKDYP
                     LRKYQLFTTPEQIPMELLRPAPGEVKSVEDSDEIFDDSRMYVNLGPSHPATHGTLRVM
                     ARLNGETIEKSISEMGYLHRAKEKIAESTTYHKFIPHTDRMNYCSALANNVVYTMGVE
                     RLMGLEIPERAKYIRTICVEIARIIDHLICVGINAVDLGAFSFFLYGFHQREEAYTLI
                     EMLCGARLTTSYTRVGGLAIEPPPEFLPALKKWIENFEPVIAEMDKLLTTNKIWMDRT
                     VGIGAFSKEQALAYSFTGPCARASNINIDLRRDQPEPLLKYDAVSFDVPIGRNGDVYD
                     RYLIRMEEIRQSVSILKQLVEKCPTTGSVWVDDKRVVIPPKERVHNSMEALIHHFKFF
                     MEGFDVPPGEAYTCIEGPNGEVGFYIQSKGGPRPWRTRVRSPSFWMYQSLDPMIKGQK
                     IADMVASLGSINIIAGELDR"
     gene            complement(2815..3309)
                     /gene="nuoB"
                     /locus_tag="PLH57_05315"
     CDS             complement(2815..3309)
                     /gene="nuoB"
                     /locus_tag="PLH57_05315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRK02064.1"
                     /translation="MAKDGSPEIITTRLDAVINWGRKNSLWPLPFGTSCCGIEMMASL
                     SADYDMSRFGAEVVRFSPRQSDLLICAGTIVNKMAPVLRNIYDQMSDPKWVMAFGVCT
                     SSGGIYNSYSVLQGIDMIIPVDVYIPGCPPNPEGVIHGLIKLQERIARGDYNDRNRLQ
                     QRSV"
BASE COUNT          877 a          842 c          707 g          940 t
ORIGIN      
        1 ttcccttcga ggctgtttaa taatccggtt tcctcaccac aaatataagc tccggcacct
       61 cgatgaacgt aaagatcaaa cttaaagccc gagcccatgg cgttctttcc taaaattccc
      121 gctttgtaag cttcgtctat cgcatgttct aaaaactcag cctcccgaac atattctcct
      181 cgaatataaa tatagccaac atgagccccg atcgcgtgag ccccaatcag cattccttcg
      241 atcaacatat gaggggagta ggtgataatc gctctgtcct taaaagtccc gggctcagat
      301 tcgtcgcaat tgcaaacaag gtactttggg gcttttgaat cttttggaat aaaactccac
      361 ttcataccgg taggaaatcc tgcgccaccc cgtccacgaa gattggattt ttttacttct
      421 gcgatgacat cgtccacagc catgcccagc gcttttttag ccgccgagta tccgccgtct
      481 ttctggtaaa aactcaaagc cgcggcttct ttattataaa aatatttcga gacgattttt
      541 tctgccattt aagaaaccat ccttatttta gctcactcag gattcggtcc attttttcgg
      601 tagtcagatc ttcataatag tctttattga tctgaacaca cggggcggtt ccacatgcac
      661 cgagacactc tacctcggta attgtgaact tattgtcgcg agtggtctcg ccggcctgaa
      721 ttccgagctt cttttcgcaa tgttcaagta gtttttcggc gcccataagt gcacaggaaa
      781 tattagtgca gacttggaga tgatagtttc caactggctt gaggttgtac atcgtataaa
      841 aagtgattac ctcgcgcaca acgggaggcg gtatatcgat ggctgctgca atttcgtcca
      901 tcgtgtcacc agacacccaa cccaactcat cttgtgctaa gtgcaatgcc ggcaataata
      961 acgcctgatt ggtagggtag cgagggcgaa gcctgtctat ttctttgtaa aatttgtcgg
     1021 acaattgttt cttcatactt tgcactccga tcaacgatcg agctcacccg cgatgatgtt
     1081 gatacttccc aaagatgcga ccatatctgc gattttttgg cctttaatca ttgggtctaa
     1141 actttgatac atccaaaacg acggtgatcg aacacgcgtg cgccacggac gcggaccgcc
     1201 cttgctttga atataaaaac ctacttcgcc attagggcct tcaatgcagg tgtaggcctc
     1261 tcccggtgga acgtcgaaac cttccatgaa aaatttaaag tgatgaatta aagcttccat
     1321 cgagttgtgt actcgctctt tcggaggaat aacaacacgc ttgtcgtcca cccacacgct
     1381 tcccgtagtc gggcattttt ctactaattg cttcaatatg ctgacgcttt gacgaatctc
     1441 ttccatgcga atcaagtaac gatcataaac gtcgccatta cggccaatcg gaacgtcaaa
     1501 agataccgcg tcgtatttaa gcaacggctc gggctggtct cgtcgcaaat cgatattgat
     1561 attgctcgct cgagcgcagg ggcccgtaaa agagtaggct agtgcttgtt cttttgaaaa
     1621 agcgcctatt cccactgttc gatccatcca aatcttattg gtggtcaaca gcttatccat
     1681 ctcagcaatc actggttcga aattttcgat ccactttttt aatgccggta aaaactcggg
     1741 cgggggttca attgcaagcc cacccacgcg cgtgtaactg gttgtcaggc gtgcaccaca
     1801 aagcatttca attaaggtat aggcctcttc gcgctggtga aacccataaa gaaaaaaaga
     1861 aaacgcacct agatcgactg cattaatacc tacacaaatt agatggtcta ttatgcgagc
     1921 aatttccacg caaatagttc gaatgtattt tgcgcgttca ggaatttcga gccccattaa
     1981 tcgctcaaca cccattgtgt atacaacatt gttcgctagt gccgagcagt agttcatgcg
     2041 gtcggtgtga ggaataaatt tgtggtacgt ggtactttcg gcaatttttt ctttagcccg
     2101 atgtaaataa cccatttcac ttatggattt ttcaatcgtt tcgccgttca aacgagccat
     2161 cactcttaac gtgccgtgag tggcgggatg tgaagggccc agattcacat acatgcgcga
     2221 gtcgtcaaaa atttcatcag aatcctcaac ggactttacc tcgcccggag caggtcgaag
     2281 tagttccatt ggaatttgtt ccggagtggt aaataattga tactttctaa ggggataatc
     2341 ttttcgaaga gggtggcctt cccagcgtat atctaacaaa attcggcgta aatctgggtg
     2401 accgtcaaat ttaataccaa acaaatcgaa aacttcgcgc tccgcccaat tagccgctgc
     2461 ccacaatggt actgccgtag gcgcggattc ctggtctttt agtctcagct taattcgaat
     2521 gcgattcaaa tttctcaagg aaaacagatt ataaactaag acaaaccttc cagatccgtc
     2581 ttcgccactt ccaagatcat cgtatgctgt gagatccgcc aaaaaattaa actcgagacc
     2641 atctgcttct tttagaaatt tcaaaacaac cattaaactg gttttggaca cccaaatcac
     2701 tgggcaccct gcacggtctt tttcggtttt taaaatggcc gcaccttggg ctgcaactat
     2761 gcgctcaagt tgctgatctg tggcgttcgt tttttcggca aagtatgcca tggatcaaac
     2821 actcctctgc tgcaatcgat tgcgatcgtt gtaatcgccc ctagcgatcc gttcttgtag
     2881 tttgattaat ccgtgaatca cgccctcggg attgggtggg cagcctggaa tatagacatc
     2941 gacaggaata atcatgtcga ttccctgcaa cacgctatag gaattgtaga ttcccccaga
     3001 tgaggtgcag actccaaaag ccataaccca tttagggtcg gacatttgat catatatatt
     3061 tcgcaaaacc ggcgccattt tgttgacgat tgttcctgcg caaattagca ggtcactctg
     3121 cctcggtgaa aaacgcacta cctcagctcc gaaacgagac atgtcgtaat cagcggatag
     3181 gctggccatc atttcaattc cacaacacga cgtcccgaaa ggcaggggcc aaagtgagtt
     3241 ttttcgcccc caattgatca cggcgtctaa cctggttgtg ataatttctg gtgatccgtc
     3301 tttcgccata ccgttactcg ctttctaatc ccactccaag acgcccttgc gataaatgta
     3361 gatata
//
LOCUS       DAOMXO010000205         3364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15687_length_3364_cov_4.388033, whole genome shotgun sequence.
ACCESSION   DAOMXO010000205 DAOMXO010000000
VERSION     DAOMXO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15687_length_3364_cov_4.388033"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PLH57_05320"
     CDS             <1..852
                     /locus_tag="PLH57_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02065.1"
                     /translation="TPASILSHPVKPKPQTLPNAKTNWAAPKIFSQGAAEITKTLKRY
                     FKRPSKTTGERAASTGKVLDFAQRSRHILRENLREFAVAASLALVFTGTYALVKTFSN
                     EQTQRQPAATGATPKSKPVTSSKPFAAEQAEEIGKGSAPIQRPRGSAGAKKPTSVLNE
                     IAPLAAPAPTEPPANAVNQNTDNQGSGPLYYSTSEDYPQEQVVTDDTQTTPGEAAYPG
                     PNSAYSGTDPTSSDPAAMQAVTGNRSAYPDPPIPEGVPPPVGDDAYRTPTSDGTTDGT
                     NGQSEDY"
     gene            935..1594
                     /gene="tmk"
                     /locus_tag="PLH57_05325"
     CDS             935..1594
                     /gene="tmk"
                     /locus_tag="PLH57_05325"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRK02066.1"
                     /translation="MKRHRKVPQKASGRFIVIEGGDGTGKSTLLNKIEHALKARAIQC
                     VRTREPGGTPLAEEIRELILAHQRPGYEIDAKTELLLYEAARAHHVRNVIRPHLAAGT
                     TVLCDRFTYSSIAYQGAARGLGEKTVAILNSIATEGLEPDLVIVLDLATKQAKARRSL
                     RGAANRLDIEPDSFHEKVRRAFLKLAKANPRRCVVLDASLDPDTVFARLLALKPWKRL
                     F"
     gene            1591..2553
                     /locus_tag="PLH57_05330"
     CDS             1591..2553
                     /locus_tag="PLH57_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HRK02067.1"
                     /translation="MNLKPLFQDDAWNTLRSMAHPDPLRLPGSIIFLGPEGVGKKRTA
                     RALFQLIHCLQPAEFEACGACRTCMLVANGNHVDLVEVEPKGSTIHVEELREVLKSNA
                     FRPYEGRIRLILIDQAHLLGVSAANVLLKSLEEPPEYVKFVLITHERTQLLPTITSRS
                     QFVYFRPLEEAAIWEIAERQSLALPSELKGVFSALLGGGLARLHTLAQADSLEFLKKA
                     RQWLQSSGQGGRRSWQETVQFADQLSALSDEQWTALFALASCECLKAATDRAAEGQLA
                     MAYNWAQRGLRFSQFEQGFEANANKKLVALLVSQALSGEHVAGP"
     gene            2550..3329
                     /locus_tag="PLH57_05335"
     CDS             2550..3329
                     /locus_tag="PLH57_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK02068.1"
                     /translation="MIHVTHLSKWFPNQPVLNRVSFSVDEGESLVVLGQSGIGKSVLL
                     KTLSRLIEPDEGFAEIRTRNIGIVFQKNELFDSLSAYENLDFVMRERTRETAPARRNK
                     IMNYLEWVGLAGSAHLMPNELSGGMQKRLAIARTLIVEPQVVLYDEPTAGLDPITSRL
                     IAELILRLKKEAGSTLVTVTSDVQRAFQLGTKVGILVRTALGAELLMAGSPEEARASS
                     DERIQQFLKGATRGPLTTKITASSESRNNIANNDFWDGDVL"
BASE COUNT          861 a          973 c          832 g          698 t
ORIGIN      
        1 actcctgcca gcatcctatc gcaccccgtc aaaccaaaac cccagacgct gccaaatgcc
       61 aagactaatt gggctgcacc gaaaattttt tcgcaaggtg ccgctgaaat taccaaaaca
      121 ttgaaacgtt attttaaacg cccgagcaaa accacgggcg aacgcgcggc ctcaaccggc
      181 aaagttctgg actttgctca aaggtctcga cacattcttc gcgagaattt gcgagagttc
      241 gcagtagcgg cgtcgctagc cttggtattt actggtacct atgcgttggt caaaacattt
      301 tcaaacgaac aaacccaacg tcagcccgcg gcaaccggtg ccacgccaaa aagtaaaccg
      361 gtaacgtcgt caaaaccgtt tgcggcagag caggccgaag aaatcggcaa aggaagcgcg
      421 ccgatccaaa gaccccgagg atcggcggga gctaaaaaac ccacctcagt tcttaacgaa
      481 atcgcaccgc ttgcggcacc ggctccgacc gagccgccgg ccaatgccgt gaatcaaaac
      541 accgacaatc agggctcagg tcccctctac tacagtacgt ctgaagatta cccgcaagaa
      601 caagtcgtaa ccgatgatac tcagaccacg cccggcgagg ccgcgtatcc cggtccgaac
      661 tcggcttatt ctggcaccga cccaacgtct tcggatccgg cggccatgca agcagtcacc
      721 ggcaaccgta gtgcttaccc tgatccaccg atcccagagg gcgtgcctcc tcccgtcggg
      781 gacgacgcct accgcactcc aacctcggac ggcaccaccg atggaaccaa cggtcagtcc
      841 gaagactact aaaacgacct gaatcgatcg ccctttcggt ccgtcggttg acgggttaaa
      901 ccccctcgcg ctagtctcgg cactcaatac gatcatgaaa cgacatcgca aagtccccca
      961 aaaagcgtcc ggccgtttta ttgtaattga aggcggcgac ggcaccggaa agtccactct
     1021 gctaaacaag atcgagcacg cgttaaaagc acgtgcgatt cagtgtgtac gaacgcgaga
     1081 acctggcggc acaccactcg cggaagaaat tcgcgagctt attcttgctc accagcgtcc
     1141 gggctatgag atcgatgcca aaacagagtt actgttatac gaggccgcac gcgcacacca
     1201 tgtgcgaaat gttattcgcc cgcacctagc agcgggaacc actgtgctct gtgatcgttt
     1261 cacctactcg tcgattgcct atcaaggcgc ggctcgaggt ttgggagaaa aaaccgtggc
     1321 tatcttaaac tcgattgcca cagagggcct tgaacccgac cttgttatcg tgttggatct
     1381 cgccaccaag caagccaaag cccgccgcag tctgcggggc gccgcaaatc gactagacat
     1441 tgagcccgat agttttcatg aaaaagtgcg ccgcgctttt ttaaaactcg cgaaagcaaa
     1501 cccgcgcagg tgtgtcgtac tcgacgccag tcttgacccc gacaccgtgt ttgcaaggtt
     1561 gctcgcactc aaaccctgga agaggttatt ttgaatctca aacccctctt tcaagatgac
     1621 gcctggaaca ccctgcgctc aatggcgcac cccgaccctt tgcggctacc agggtcgata
     1681 atattcctcg gcccagaggg ggttggaaaa aaacgaaccg ctcgcgcgct atttcagttg
     1741 atccactgcc tgcagcctgc ggaattcgag gcctgtggcg cttgccgtac ctgtatgctt
     1801 gtggccaatg gcaatcacgt tgacctagtg gaggtggaac cgaaaggttc taccattcat
     1861 gtagaagaac tacgcgaagt gctaaaatca aacgcatttc gaccttacga aggtcgaatt
     1921 cggttaattc taatcgacca agcacatcta ctcggtgttt cggctgccaa cgtactgctc
     1981 aagtcgctcg aagaaccacc cgagtacgtg aagttcgtgc tcatcactca cgaacgcacc
     2041 caactgctcc caactattac cagccgcagc caattcgttt attttcgtcc gctcgaagaa
     2101 gcggctattt gggaaatcgc cgagcgccaa tcgcttgcat tgccctcgga attaaaagga
     2161 gttttttccg cactgctcgg tggggggctt gcccgacttc atacgctcgc ccaggcagat
     2221 tcactcgaat ttctaaaaaa ggcccgtcag tggctacagt ccagcggaca aggcggacgc
     2281 cggagttggc aagagaccgt acagtttgcc gaccaattga gcgcgctgag cgacgaacaa
     2341 tggaccgcat tatttgcgtt ggcctcctgc gagtgtttaa aagcagcaac cgatcgtgcg
     2401 gccgaaggtc aactcgcaat ggcctataac tgggcccaac gtggacttcg cttttcgcaa
     2461 ttcgagcaag ggtttgaagc caatgccaat aaaaaactag tcgcgttgct cgttagccaa
     2521 gcactcagcg gggaacatgt ggcgggccca tgattcacgt cactcatctt tctaagtggt
     2581 ttccaaatca accggtactt aatcgcgttt cgttttcagt cgacgaaggc gagagtcttg
     2641 tcgtgctcgg ccaaagcgga ataggcaaat cggtccttct taaaacactt tctcgactaa
     2701 tcgagcccga cgagggattc gccgaaattc gaactcgcaa cattggaatc gtttttcaga
     2761 aaaatgaact tttcgatagc ttaagcgcct acgagaatct cgatttcgta atgcgagaac
     2821 gtacacgcga aacggctccc gcacgtcgta ataaaatcat gaattacctc gaatgggtgg
     2881 ggctcgcggg ctctgctcat ttaatgccca atgaactttc ggggggtatg caaaaacgtc
     2941 tggccatcgc tcgcaccctg attgtcgaac cacaggtggt gctctatgac gaaccaactg
     3001 ccggactcga ccccattact tcgcgcttga ttgccgagct catattgcgc ctaaaaaaag
     3061 aagctggttc cacgctcgtg acggtaacca gcgacgtcca gcgcgcattt cagctgggaa
     3121 ccaaagtagg cattctcgtg cgcacggccc tcggagccga gttgttaatg gccggctctc
     3181 ccgaagaagc ccgagcctca agcgacgaac gcattcaaca gtttttaaaa ggcgccacaa
     3241 gaggccctct cactaccaaa attacggcca gctcggagag tcgcaacaat atagcaaata
     3301 acgatttctg ggatggcgat gtcttataag aatgtcgcaa aggcgtacgg ggaaacgaaa
     3361 gtat
//
LOCUS       DAOMXO010000206         3361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15711_length_3361_cov_4.701149, whole genome shotgun sequence.
ACCESSION   DAOMXO010000206 DAOMXO010000000
VERSION     DAOMXO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15711_length_3361_cov_4.701149"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLH57_05340"
     CDS             <1..628
                     /locus_tag="PLH57_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRK02069.1"
                     /translation="DDTPAITVLEMRSRCRRLVTEHGLNLIIVDYLQLMRGNSKGMKG
                     ADSREREISEISRSLKALAKELRVPVIALSQLNRSVESRPDKRPMLSDLRESGAIEQD
                     ADIVAFVYRDEVYNKETEDKGIAELILAKHRAGSVGTVRLSWQAEFTAFGNLAPDYYS
                     QDSTMAQQGQSFSADSGSSGRAKSPGLDLRDQGLIPIPKKGPLDLPNF"
     gene            complement(625..948)
                     /locus_tag="PLH57_05345"
     CDS             complement(625..948)
                     /locus_tag="PLH57_05345"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRK02070.1"
                     /translation="MITDTGENMNTKTKETLDKILGQAKESVKVLELLQHEAVSRAKS
                     VVANLPNEKIAEAFRKMGFATRAEVEALEQKVETLAEEVATLTSKLQQKKSPKDRTKD
                     QESSI"
     gene            1060..1593
                     /locus_tag="PLH57_05350"
     CDS             1060..1593
                     /locus_tag="PLH57_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02071.1"
                     /translation="MKKLSILLILIAMGWAESGMAAKKRGAQVMSSSSDDSYSSSSYS
                     RSSSNNELLMDLTINYLRGTQPDQATDTAPRFSIGGMFTDWVGLDFQGIYTARSKNFL
                     VGADLRLAPVEWLYVKGGIGGYSDKATRQFTVTPLAGTGIRVHLTQKSYFVTEATYYQ
                     VNREEFIGIGAGLGVSF"
     gene            1664..>3361
                     /locus_tag="PLH57_05355"
     CDS             1664..>3361
                     /locus_tag="PLH57_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02072.1"
                     /translation="MGHRDQKKVCQFLLLILAATLFGCSSSLFTGDTQSDQAVGDGSF
                     NKGCLGVDLSQETLSVRSTRGLMGCLNANGSIQAYSDLLNSISDDELLGLVSVVNKEL
                     LTNPDRLKEVTVSLDQMDAAGVLDRTLKSAGSFLENADFLSSVLKAIQLGGQNPDIQD
                     HPSIDPEILTTIAQLTKELDSGAGAIANTAKGFDVGISAVGLPSFQEIVTGLRPEIED
                     GPTLAQIVEQVHVYVQAKQDPKEPKLLSVLLEGIADRSIFYGLDHFYYDECEGARPGD
                     CADRPRLSLDHQIASMEAFVAFLTRERRDVLQRLTKLFGVMNRPIRCLGDTKEIPNAN
                     LYTMDQLVSRDPLLVPSWVVRENMTKLKLASGMCDYPAEFDQLMGVLRELADPELAGR
                     SRGGAARDEHMLVTVAHFVQGLRVGDEFGARSNLDRHRFRKFLINWLGDLSDVSPASH
                     LANGLAELSRSDRRIIANLLYFLNAPDDMDRDTLANLISVLLKPRSELSGQSIYQVVS
                     GVVTRLDSSTVYDVVKTLPHYIRSEQALLEPLLSANRDAFHMNDAHPFVNLVLAVGKN
                     "
BASE COUNT          819 a          788 c          837 g          917 t
ORIGIN      
        1 cgatgacact cctgccatta cggtgttgga aatgcgttcg cgttgccgaa gattggttac
       61 cgaacatggt ctgaacctaa ttattgtgga ttatttgcaa ctaatgcgtg ggaattcgaa
      121 gggcatgaaa ggcgccgatt ctcgggaacg cgagatttcg gaaatttcta gatcgctaaa
      181 agcgttggca aaggaattgc gggttccggt tattgccctc tctcagctca accgaagcgt
      241 ggaaagtcgc cctgacaaac gacccatgct cagtgattta cgggaatcgg gcgcgatcga
      301 gcaagatgcc gatatcgtgg cttttgtgta tcgcgacgaa gtttataaca aggaaaccga
      361 agataaaggc attgccgaac tgattctcgc caaacaccgc gcaggcagtg tgggtactgt
      421 acggctttca tggcaagccg aattcacggc tttcggaaat ttagcaccag actattactc
      481 gcaagactca accatggcgc aacaagggca gtcgtttagt gctgattcgg gttcttccgg
      541 tcgcgcgaaa tccccgggcc tcgatcttcg tgaccaggga ttgataccca tacctaaaaa
      601 aggtcctctc gatctaccta atttttaaat cgaggattcc tggtctttag tgcggtcttt
      661 agggcttttt ttttgctgaa gtttcgacgt tagtgtcgct acttcttcag ccaaggtttc
      721 gactttttgt tccaaagctt ccacttcagc ccgagtagca aaacccattt ttcgaaaagc
      781 ttctgcgatt ttttcgttag gtaggttagc tacgaccgat ttcgctctgg aaacggcttc
      841 gtgttggaga agttcgagca cttttaccga ttccttcgcc tggcctagga ttttgtccaa
      901 agtttctttt gtctttgtat tcatattttc acctgtgtca gtgatcatcg tccgatttaa
      961 cccattttac aaagggcaaa tcttgcctct atcagaaact tttagaggcg ctattctaag
     1021 agagagttgg ccgtgggttc atttgctaga gggcaagaca tgaaaaaact ttcgattctc
     1081 ttgatattga tcgcaatggg gtgggccgaa tccggaatgg cggcaaaaaa aagaggcgcc
     1141 caagtcatga gttcttcgtc ggatgatagc tactcctcaa gttcttatag tcggtcttca
     1201 tcgaataacg aacttctcat ggatcttaca attaactacc ttcgtggtac ccaacccgac
     1261 caggccaccg atactgcacc tcggttcagt attggaggga tgtttacgga ctgggttgga
     1321 ctcgactttc agggtattta caccgctcgc tccaaaaatt ttttagtggg cgccgacctt
     1381 cgtttggcgc cagttgaatg gctgtacgta aaaggtggaa taggtggcta ctccgacaaa
     1441 gccacccgcc agtttacagt aaccccgctt gcaggtactg gaattcgcgt acaccttact
     1501 cagaagtcat acttcgtaac cgaagcgact tactatcagg ttaatcgcga agaatttatc
     1561 ggaattggtg cgggtctggg cgtaagcttc taactaattc ggtttattgc cccgctcatt
     1621 gactcccgct ctcgattcac ggtaaatcta tgtgcggggc gttatggggc atcgggacca
     1681 gaaaaaggtt tgtcagttcc ttctactgat cttagcggcc accctattcg ggtgttcctc
     1741 ctcgttattc accggagata cccaaagcga tcaagccgtc ggtgacggta gttttaataa
     1801 gggctgtctc ggggttgatc tctcgcaaga aactctctca gttcggtcca ctcgcgggct
     1861 aatggggtgt ttaaacgcca atggctccat tcaagcttat tcagatttac taaactcgat
     1921 cagtgacgac gaactcctcg gcctagtgtc ggtggtgaac aaagaacttc tgacgaatcc
     1981 agatcgattg aaagaagtca cagtttcgct cgatcaaatg gacgcagccg gggttctaga
     2041 tcgcacgctc aaaagtgctg gctcgtttct cgaaaatgca gatttccttt cgagtgtttt
     2101 aaaggcaatt cagctgggcg ggcagaaccc agacattcag gatcacccat cgattgatcc
     2161 cgaaatatta actacaattg cgcagctaac taaagagttg gacagcggtg ccggtgccat
     2221 tgcgaatact gccaaaggtt tcgatgtcgg aataagtgct gttggattgc cgtcgtttca
     2281 ggagatcgtc accggtttgc ggcccgaaat tgaagacgga ccaacattag ctcaaattgt
     2341 agaacaggtt cacgtttatg ttcaggctaa acaagatcca aaagaaccga agcttttgag
     2401 cgtactgctc gagggaatcg cagatcgatc gatcttttat gggcttgatc atttttatta
     2461 tgacgaatgc gaaggtgcgc gccccggtga ttgcgccgat cgtcctcgct tgagcttgga
     2521 tcatcaaatc gcatcgatgg aagcgtttgt agccttttta actcgtgagc gacgcgatgt
     2581 tttgcagcga ctaactaaat tgttcggtgt catgaatcgc cccattcgat gcctcggaga
     2641 taccaaagaa attccaaacg ccaacctcta tacgatggat caactggttt cccgagaccc
     2701 actgctggtg ccttcgtggg tagtgcgcga aaacatgacc aagctaaaat tggcttctgg
     2761 catgtgtgat tatcccgctg aatttgacca actcatgggc gttttacggg aattggcgga
     2821 tcccgagttg gccggaagat cgcggggggg cgccgcgcgc gacgaacata tgttggtaac
     2881 cgtggctcat tttgttcagg gtttgcgggt gggcgacgaa ttcggggcac gctcgaacct
     2941 cgatcgacac cgttttcgaa aatttcttat caactggttg ggggatcttt cagacgtatc
     3001 gccggcgagt cacttggcaa acggactagc agaactttca cgaagtgatc ggcgaattat
     3061 tgcaaatctt ttatattttc ttaatgctcc agacgacatg gatcgcgata cactcgcgaa
     3121 tttgatttcg gtactattga aaccacgttc agagttgagc ggtcagtcaa tttaccaggt
     3181 agttagtggc gtggtaacgc gattagactc ttcgaccgtc tacgacgtag ttaaaactct
     3241 acctcattac attcgcagcg aacaagcatt gctagaacca cttttgagcg caaatcgtga
     3301 cgcatttcat atgaacgatg cccacccttt cgtgaattta gtgctcgcgg tcggaaaaaa
     3361 t
//
LOCUS       DAOMXO010000207         3359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15722_length_3359_cov_5.596550, whole genome shotgun sequence.
ACCESSION   DAOMXO010000207 DAOMXO010000000
VERSION     DAOMXO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15722_length_3359_cov_5.596550"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PLH57_05360"
     CDS             <1..821
                     /locus_tag="PLH57_05360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK02073.1"
                     /translation="GYDVFLKRTNFRDQVEIAVGNYIEAKFHRFVGPSGSTRILEIGC
                     GNGEMTVRYLRELRNVLQSVAPKVAANFKIRIDLVEPAQGALNEAVRAIKELDPNIEV
                     RAFAETMDAFKENAAFRDSEYDWMIGSYVFYHVSPTIIPGLLEKLSPLGTFMVAMGSP
                     QHPLRAPAPLKALSKHGDSNAVASVLDSSAVRSKFGVELLNVDTEVDLKGLWNQSTGA
                     TTDGTAYFSFIYNHDLQRFNNEERAALDGLLTNIFNEQGGKIHPQHHLFWVWRK"
     gene            809..2998
                     /locus_tag="PLH57_05365"
     CDS             809..2998
                     /locus_tag="PLH57_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRK02074.1"
                     /translation="MAKIKLLLSIIAFASSAAFAAPPSLTDWQYRVYKSVSEAERLPW
                     KPAQELRLSREYQGFIEYRARIDLSVYSDKSKENELGVFLGRISDVDRTYVNDVLIGE
                     TGSFPPHYENNMDVVREYRIPREALGTSSVVTLRVLAYVEYPSSKGLNLSTVEIGFHR
                     DLQQKKYVADLSWYGSRMFIPVLCLLLAAISLPWLAPVGLRFQQAVILLLGVTCFIFG
                     ICRSRVVFHFMDIVSAYKLTGMSSVLAVTLICWYAVLQAKIGRQRVRLATLLALSLPV
                     LFFWPVFLSAESVLVVAGVMKRWFFVEAVMIWAAAVFVIWRAGSGRFWLKLGFLFLAA
                     VITNDILRDLRVFNLPILMDIGFGVFLVTLVISQLVSLRMSWSALARREARLEHDVRL
                     GRVSGLLSHDLRNKIGAVSVLVDSTALAPETKDKIASLLGRIKGSVELHLKQAVADPA
                     PSSRQVEMSAPEAFEQPACSLGGVVQTCIEDLKMSHSVELIADIARKAFTAFVPIEYS
                     EMSRCATNILLNAIEASSAASPTSKIYVSAHVSDTGCEIVVKDQGAGFSDEALRAALS
                     GNQMTTKPGGTGLGVISARTILARIGGELKITTSAAGTVVRLILPRVAPPAWFFNIGN
                     HVAKELIVLDDDTNIHEKVRRLFPEKTVFATTREDEFIKLAQEMPNALVLVDFDFGGT
                     RSGLDLIASEPALRQGVLVSGRISFDRDLRGSALDRGVRMYPKECLG"
     gene            complement(3091..>3359)
                     /locus_tag="PLH57_05370"
     CDS             complement(3091..>3359)
                     /locus_tag="PLH57_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02075.1"
                     /translation="TDQISKVESPVTETYFRQNVRPKERGLLLLYALNPHLEMTDETE
                     KGHIESLSQTMPIRAASNAIAVAFVFPRTSNSKSTYSYIVNGTV"
BASE COUNT          779 a          795 c          874 g          911 t
ORIGIN      
        1 cgggatacga cgtctttctg aaaagaacaa attttcggga tcaggttgaa atcgctgttg
       61 gtaattacat cgaagcgaaa tttcatcgtt tcgtgggtcc gtctggatca acacgaatct
      121 tagaaatagg ctgcggcaat ggcgagatga cagtgcgtta tcttcgggaa cttcgaaatg
      181 ttcttcaaag cgtggctccg aaggtggcgg caaattttaa aatacgcatt gatctcgtag
      241 agccagcgca aggtgctttg aacgaggccg ttcgcgccat taaggaactt gatccaaata
      301 ttgaagttcg ggcattcgca gaaacgatgg atgccttcaa agaaaatgct gctttcagag
      361 actcagaata tgattggatg attgggagtt acgttttcta tcacgtcagc ccaactatta
      421 tcccgggcct actcgaaaag ctttctccac tcggtacttt tatggtggct atgggttccc
      481 cgcagcatcc attacgagct ccagctccat tgaaggctct ttccaaacat ggtgattcaa
      541 atgccgttgc ttcagttctg gacagcagcg ctgttcgatc aaaatttggc gtagaattac
      601 tgaacgttga taccgaggta gatctgaagg ggctatggaa tcagtctact ggagcaacga
      661 cagatggaac cgcatacttt tcattcatct acaatcatga cctacagcga ttcaataacg
      721 aggaaagagc agctctcgac gggctgctca caaatatctt taacgagcag ggtggaaaaa
      781 ttcatcctca acatcactta ttctgggtat ggcgaaaata aaactcttac tcagcatcat
      841 cgcttttgcc tctagtgcag cattcgccgc gccaccttca cttactgatt ggcagtatag
      901 ggtgtacaaa agcgtctctg aagctgaacg gttgccctgg aagccagcgc aagagctgcg
      961 tctttcacgg gagtaccaag ggtttattga gtatcgcgcc cggattgatc tgtcggtgta
     1021 ttccgacaaa tccaaagaaa atgagttggg tgtatttctc ggcagaattt cggacgttga
     1081 tcgaacttac gtcaacgatg ttctgatagg cgaaacaggc tcatttccgc cgcattacga
     1141 aaataatatg gacgttgtgc gtgagtaccg cattccccgt gaagctcttg gtacaagcag
     1201 cgtcgtgacg cttcgtgtgc ttgcctatgt tgagtatcct tcgagcaagg gactcaatct
     1261 cagcactgtc gagatcggat ttcaccgtga tcttcagcaa aagaaatacg tcgctgatct
     1321 ctcttggtac ggctcgcgca tgttcattcc agtcctctgt cttcttctcg ccgcaatttc
     1381 tcttccttgg ttggctcccg tggggttgcg ttttcagcag gcagtcatct tacttctggg
     1441 agtgacttgc ttcatttttg gaatttgccg gagtcgcgtg gtctttcatt ttatggatat
     1501 cgtgagcgcg tacaagctaa ccggcatgtc gagcgttctc gctgtgacgc ttatttgttg
     1561 gtacgccgtg cttcaagcga aaattgggcg gcagcgtgta agactcgcga ccttgcttgc
     1621 tctttctctt ccggtgctat tcttttggcc cgtatttctt tcagcggaga gcgtacttgt
     1681 tgtagccgga gtgatgaaac gctggttctt tgtagaagct gtaatgatct gggctgccgc
     1741 agtgtttgtg atctggcgtg cgggctcggg gcgcttctgg cttaagcttg gttttttgtt
     1801 tttggccgct gttattacga acgatattct gcgcgacctt cgtgtgttca atcttccgat
     1861 tctaatggac atcggctttg gcgtgtttct cgtaacactc gtgatttcgc aactcgtttc
     1921 attgcgaatg tcgtggagtg ctctcgcgcg ccgcgaagca cggcttgagc acgacgtaag
     1981 gctcgggagg gtctcggggc tactcagtca cgatctccga aataaaatcg gcgcagtttc
     2041 ggtgctggtt gactcaacag cgcttgcgcc tgagaccaaa gacaaaatcg cttcattgct
     2101 gggccgcata aagggcagcg ttgaactgca ccttaagcaa gctgtcgccg accctgcgcc
     2161 gtcgagtcgc caggttgaaa tgagcgcacc ggaggccttc gagcaacccg cgtgcagcct
     2221 gggcggggtg gttcagacct gcatcgaaga cctaaaaatg agtcattcgg tcgagctgat
     2281 cgccgatatt gccagaaaag cattcaccgc ctttgttcca atcgaatatt cggaaatgtc
     2341 ccgctgcgcg acgaacatac tcctcaatgc tatcgaggct tcgtccgctg cctcaccgac
     2401 gtcgaaaatc tatgtgtctg cgcacgtttc ggataccggg tgcgagattg ttgtgaaaga
     2461 ccaaggggcc ggattttctg atgaagcttt gcgtgctgcg ctttcgggta accaaatgac
     2521 gacgaaacct ggcggcacgg ggttaggggt tattagtgca cggacaattc ttgctcgcat
     2581 tggtggagag ctcaaaatta caaccagcgc tgcgggaaca gtggttcgcc taatcttgcc
     2641 gcgagtggct ccgccagctt ggttttttaa tatcggaaat cacgttgcca aagaactaat
     2701 cgttctcgat gacgacacaa atattcatga aaaggtgcga cgcctttttc ctgaaaaaac
     2761 ggtttttgcc accacgcgag aagatgagtt cataaaactc gctcaagaaa tgccgaacgc
     2821 cctggtgctc gtcgattttg attttggcgg gacaagaagt ggccttgatc tcatcgctag
     2881 cgagcctgcg ctgcgccagg gagtgcttgt gtctgggcga atttcattcg atagggatct
     2941 ccggggttcc gcgctcgatc gaggcgtgcg aatgtatccg aaggaatgcc tcggctgaag
     3001 cagtcgttgg caattttgac cctaccaaag aaaaaaacta ttacctaaag tagagcccca
     3061 aacttaagta acccctttcg ggagctgaac tcaaactgtt ccgttaacaa tatagctgta
     3121 tgtgctcttc gaattgctag ttcgcggaaa tacgaaggca acagcaatgg cgttcgaggc
     3181 cgcccgaatt ggcatggtct gagaaagcga ttcgatatga cctttttcgg tctcgtctgt
     3241 catttctaag tgcggattaa gggcatagag taataagagt cctcgctctt ttggtcgaac
     3301 attctgccga aagtaagttt cagttaccgg actttcaact ttcgaaattt ggtcggtat
//
LOCUS       DAOMXO010000208         3343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15825_length_3343_cov_4.703163, whole genome shotgun sequence.
ACCESSION   DAOMXO010000208 DAOMXO010000000
VERSION     DAOMXO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15825_length_3343_cov_4.703163"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PLH57_05375"
     CDS             <1..870
                     /locus_tag="PLH57_05375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRK02076.1"
                     /translation="CVVAEKKKKLTLTEPIQTFFPDFPSNKVTVSHLLNHSSGLLAHL
                     DLHADFHAPDGLGDFDAFSSPILARVKYEEKIIATWRPDLFEKECVYSDFGFMLLGWM
                     LEQIGQMPLDALFQEWVAQPAGISELQFLPITPNVLPTEDCPWRGRVLRGEVHDDNCY
                     VLGGVAGHAGLFGTTRAVLALAKIWLLGEKPLQKKLIQKYWNTTHVVGSSRVLGWDGI
                     SQESSTGRYFSKNSRGHLGFTGTSLWIDPDRELIVTLLTNRVHPTRRNDSIKKFRPEF
                     HDSLMHELGFARP"
     gene            900..2312
                     /locus_tag="PLH57_05380"
     CDS             900..2312
                     /locus_tag="PLH57_05380"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRK02077.1"
                     /translation="MKSFAGKKIHVIGCAGTLMGSFCAFLKRAGVEVTGSDQNIYPPM
                     SDVLAAAKVPVFTPYAVANLEKAAPDLVIVGNVVRKDNVEMVAALERGLPYLSLPSAL
                     EKMILPEKHSVVVAGTHGKTTTASLMAWCLEQCGEDPSFFVGGVLQNFPESFRVHPSK
                     FMVLEGDEYDTAFFDKVPKFTHYSPRDVILTSIEYDHADIYPNIGSVIEAFDRLAALV
                     PPSGNMVVCGESEVTLRVAHRSRGIVTSYGFGDAVDSRCSNLELGPTGSKFLWSFGDE
                     EHRLNFSMTGRHNVLNAAGVLTLCRLLDMPMDKVFSALETFKGIKRRQEVYGVVKNIT
                     LIDDFAHHPTAVRETLSALRARHPKARLVACFEPRSATSRKKVFQQAYSESFDSADLV
                     YIAKPYNATAAAPSPDTFSSEELLADLKERGRRAKQLETSATGVKQFASDLRAGDVVV
                     VMSNGGFDGLLPNLLSELGK"
     gene            2309..2791
                     /locus_tag="PLH57_05385"
     CDS             2309..2791
                     /locus_tag="PLH57_05385"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HRK02078.1"
                     /translation="MTERFYDRQLMSLFWELNFRHFAGVLPPIEVRFSGRLKTTGGQY
                     FRKPRRLIQVSTRYLSMANSWKEIADTLGHEMVHYWLDFHGRPCGHNTAFWIKLNECG
                     FQRYSRLTPVNARYVYKCPSCKVPYYRRRRGVWSCGPCSGQRFNSKYKLELVQKIAVG
                     "
     gene            2800..>3343
                     /locus_tag="PLH57_05390"
     CDS             2800..>3343
                     /locus_tag="PLH57_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02079.1"
                     /translation="MRCPTCRSYIPEGLQTKTDGSVICPNCQAPAREQPANATSGAQP
                     SDSQSRRTEVESTSTPSFAPYLTLRMKIAFGPAGALLEPSIGTLSFGPEGFSVVLDRG
                     RRWEKVYYRDLANLRSLDTDVRFDLRGVESRLSIYRASLPSWIPLGWFRKKSARTLLE
                     LLERAPKGLTAAEASLFRRRL"
BASE COUNT          789 a          867 c          827 g          860 t
ORIGIN      
        1 tgtgtggtgg cggaaaagaa aaaaaaacta actctgaccg aaccaattca gacttttttt
       61 cccgacttcc cctcgaacaa agtgacagta tcgcatttgc ttaatcatag ttccggtctg
      121 ctggcccatc tcgatctcca cgcagatttt cacgcccctg acggtttagg tgattttgac
      181 gcattttctt ctcccattct cgctcgcgtg aaatacgaag aaaaaattat cgcaacctgg
      241 cgaccagatc tattcgagaa agaatgcgtt tacagtgatt ttggtttcat gcttcttggc
      301 tggatgctag aacaaatcgg gcagatgcca cttgatgcac tttttcaaga atgggtcgcg
      361 cagcccgcgg gaatctcgga actgcaattt ctcccgatca ctccgaacgt acttcccacg
      421 gaagattgcc cgtggcgtgg ccgtgtgttg cgtggcgaag tgcacgacga caactgttat
      481 gtcctcggtg gcgtcgcggg acacgcagga ttattcggaa ccacccgagc ggtgctggca
      541 ctagccaaaa tctggttgct tggcgaaaaa cccctgcaaa aaaaattaat tcaaaaatac
      601 tggaacacca ctcacgtcgt cggcagttct cgcgttcttg gctgggatgg cattagccag
      661 gagagctcaa ctggtcgtta cttttcaaaa aattcgcgag gccacctagg atttaccggc
      721 accagtttgt ggatcgatcc ggatcgggaa ctcatcgtca cgctccttac caatcgcgtg
      781 cacccgacgc gccgcaacga ttccatcaaa aaatttcgac cggaatttca tgactcgctc
      841 atgcacgagc ttggctttgc gcgaccgtag agtgtacact ggttcctggt ggggcatcga
      901 tgaaatcatt cgcaggcaaa aaaattcacg ttataggttg tgccggcaca ctcatgggct
      961 ctttttgtgc ttttctcaaa cgagctggtg ttgaagtcac cggtagcgat caaaatattt
     1021 accctcctat gagcgacgtt ttagccgctg ctaaagtgcc agtattcacc ccttacgcgg
     1081 tcgccaattt ggaaaaagct gcgcccgatc tggtgattgt tggcaacgtc gttcgtaagg
     1141 acaatgtcga aatggtagct gcgctcgaac gcggattgcc atatttgtcg ctaccaagcg
     1201 ctctcgaaaa aatgatctta cccgaaaagc acagcgtggt cgtcgccggc actcacggta
     1261 aaacaaccac cgcaagcctc atggcttggt gcctcgagca gtgtggagaa gatccatcgt
     1321 ttttcgtagg cggcgtcctg cagaattttc ctgaaagttt tcgtgtacac ccgagtaaat
     1381 tcatggtcct cgagggcgac gaatacgaca ctgctttttt tgataaagtg ccgaagttca
     1441 ctcactactc acctcgtgac gtgattctta cttcgataga atacgatcat gctgatatct
     1501 acccaaatat tggttccgtg atcgaggcct ttgatcgctt agcggcactg gttccgccaa
     1561 gtggcaacat ggtggtgtgt ggcgaatcgg aagttactct gcgggttgct catcgttctc
     1621 gcggaatcgt tacgagttat ggtttcggtg acgcagtaga ttcgcgttgc tcaaacttag
     1681 aactcggccc cactggttca aagtttctct ggtcatttgg cgacgaagaa caccgactga
     1741 acttttcaat gaccgggcgg cacaacgtgc taaacgctgc aggcgtgtta acactttgtc
     1801 gtctcttgga tatgccaatg gacaaagttt tttccgccct agaaaccttc aaaggaatta
     1861 agcgacgcca agaagtctac ggcgtagtca aaaacattac cctaatcgac gacttcgctc
     1921 accatcccac ggcggttcga gaaactctga gtgctttgcg agcgcgtcac cctaaagcca
     1981 gattggtggc ttgtttcgaa ccgcgaagcg caacatctcg aaaaaaagtt tttcaacaag
     2041 cctattccga gagttttgac tcagccgatt tggtttatat cgcgaaaccg tataacgcta
     2101 ccgcggcagc cccatcgccc gatacgtttt cttctgaaga acttctcgcc gatctcaaag
     2161 aacggggtcg tagagctaaa caactcgaaa cttcggcaac gggagttaaa caatttgcct
     2221 ctgatcttcg ggccggcgac gtagttgttg tcatgagtaa tggcggtttc gacggacttt
     2281 taccaaattt actttcggag ctcgggaagt gacggaacgg ttttacgatc ggcaattgat
     2341 gtcgctattt tgggaactca actttcgcca tttcgcaggg gtactaccac cgatcgaagt
     2401 tcgtttttcc ggcagactca aaacaactgg cggccaatat tttcgcaagc cccgccggct
     2461 cattcaagtc agtacccgtt atctttcgat ggccaattct tggaaagaaa ttgccgacac
     2521 tctagggcat gaaatggtgc actactggct cgactttcat gggcgcccct gtggccacaa
     2581 taccgctttt tggatcaaac ttaacgaatg cggctttcaa cgctactctc gacttactcc
     2641 ggtgaacgcg cgctacgtct acaaatgtcc gtcatgcaag gtaccttact atcgacgccg
     2701 ccgtggagtt tggagttgcg gcccgtgttc gggtcagcgt tttaattcca agtacaagct
     2761 cgagcttgtg cagaaaatag cggtgggtta aaagtaatca tgcgttgccc aacctgccga
     2821 agttacattc ccgaaggtct tcaaaccaaa accgatgggt ccgtgatctg ccctaactgc
     2881 caggccccag cccgcgaaca gcccgccaac gctacttctg gtgcgcagcc gtctgattcg
     2941 caatcccgtc gaactgaagt ggaatcaacg tcgacccctt cgtttgcgcc ttatcttact
     3001 ctacgaatga aaattgcatt cggaccagca ggagctctgc tggagccatc tattggaacg
     3061 ctctcgtttg gccccgaagg gtttagtgtc gtgttagacc gaggtcgacg ttgggagaag
     3121 gtctattatc gcgatctagc gaatttgcgg tcattagata cggacgttcg attcgattta
     3181 cgtggtgttg agagccgtct gtcgatttac agggcttcac ttccgtcgtg gatcccactt
     3241 ggttggtttc gtaaaaaatc cgcgcgcacc ctgctcgagc tacttgagcg ggcaccgaaa
     3301 gggcttacgg ccgcggaagc gtctttgttt cgaagacgtc tgg
//
LOCUS       DAOMXO010000209         3338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15855_length_3338_cov_4.744441, whole genome shotgun sequence.
ACCESSION   DAOMXO010000209 DAOMXO010000000
VERSION     DAOMXO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15855_length_3338_cov_4.744441"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLH57_05395"
     CDS             complement(<1..270)
                     /locus_tag="PLH57_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02080.1"
                     /translation="MRLIAAFLLIATFFPLSGQAGILCARNAKAVPVDPVFENKNDRC
                     DGSVKVQAISYKCGSEAEAAFVMRSFLNDLQEEGEQEGAVAGGNEN"
     gene            complement(312..809)
                     /locus_tag="PLH57_05400"
     CDS             complement(312..809)
                     /locus_tag="PLH57_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRK02081.1"
                     /translation="MSMFGKKIRRREQDFYLQITSLIDTLVIILVFMLMTVGTGSVNL
                     EMASNIALPWAHAGAELPQGVKLIAKLDGIYLDQEKIVPMKDGVVPGDAVSEGGKKIV
                     GLFQKMAKLAAESKKAADESKLKFEGKVLMQADKAVPLKTVKQVLYTSARAGYNDFKF
                     AVLKQ"
     gene            complement(812..1375)
                     /locus_tag="PLH57_05405"
     CDS             complement(812..1375)
                     /locus_tag="PLH57_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRK02082.1"
                     /translation="MFHKRAHTAHDFELNLTPIIDCFVTLICFMLLSTTYVALVGLDA
                     KVPLAVPASAPEAQRKDPTFKLDLVYRKNGIDISAVGAGSLSGKRSVPHIAGKPDLTT
                     LHDTLVKIKKERPKEFTIHFNSDIDMAYADLVFLMDATRNLDPKKDGEIKVTDERNGK
                     VMLMDVLFPDFIIGGLSAAAANPAKGQ"
     gene            complement(1379..2053)
                     /locus_tag="PLH57_05410"
     CDS             complement(1379..2053)
                     /locus_tag="PLH57_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRK02083.1"
                     /translation="MILHLLPIAATGVVAAALIAERTSKLFFQYSIDSKLFMSQIEGL
                     LQKGNLQGALDLCAANEQKLLPRVVKAALLKASRNQEEIKTSLEVAVIDANSLLSARI
                     GYLAMIANVATLLGLLGTIAGLIQSFKAVASADAATKQSLLAEGISVSMNATGLGLVV
                     AIPAMIAFSLLSGRSNKILDEIEKGAGRTLSLLHNRVYAGEDDFSNVSFDTVETGNGS
                     GIKGAA"
     gene            complement(2069..3145)
                     /locus_tag="PLH57_05415"
     CDS             complement(2069..3145)
                     /locus_tag="PLH57_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02084.1"
                     /translation="MQYNKIRSTTGLIAHHGYAALLFLGLAALGSTLTSCGFRDPRIA
                     VLGRSDSTVTSGVANNKVDILWIIDNSGTMGPKQTSLRNSINSFMNSFVSKNFDYRIA
                     ITTTDTRAVDPLAPNDPNLSGQAACFVGSPTVVEASTPNPATEVSDNANVGFFGSSDA
                     HGLDAVELALSEPNLSNCNSGFFREDAYLAVIFFSDADDNTAATVSGLTTFLDGIKTP
                     FTLASGSTIRSWNASAMVVDDVNDPDCIALGPASEYGSKYIELANNTGGQVASICAPD
                     FSAGLLNVATKILEASTAIRLSRTPDPGTIAVFKNGEYLAQSATNGWTYEASINSIVF
                     HGSAIPDGPNIPINVFYTPNDIIR"
BASE COUNT          791 a          786 c          868 g          893 t
ORIGIN      
        1 gttctcgtta ccgccggcta cagcgccctc ctgctcgccc tcttcctgca gatcgttcag
       61 aaaactccgc atcacgaagg cagcctcggc ttcactgccg cacttgtaac taatggcttg
      121 aaccttgact gagccgtcgc agcggtcgtt tttattctca aacactgggt ctacgggcac
      181 ggctttggcg ttgcgcgcac agagaattcc ggcctggccg ctaagcggaa aaaaagtggc
      241 tatcagaaga aaggcagcaa tcagtctcat taagaccgat tgtagcttaa gttcgaatat
      301 aaaagggaaa actattgttt caagacggca aacttgaagt cattgtagcc tgcgcgcgcc
      361 gacgtgtaga gtacttgttt caccgttttt agtggaacag ccttgtcggc ctgcatgaga
      421 acctttcctt caaatttcaa cttcgactcg tcggcggcct tcttactttc tgcggccaat
      481 ttagccattt tctgaaagag accgacgatc ttttttccac cttcagacac tgcgtcgcca
      541 ggaaccacgc cgtctttcat gggcacaatt ttttcttggt ctaggtaaat gccatcgagt
      601 ttcgcgataa gttttacgcc ttgtgggagt tccgcaccgg cgtgagccca cgggagtgcg
      661 atattcgaag ccatttcaag gttcacggag ccagttccta ccgtcatgag catgaacact
      721 aaaatgatca cgagcgtgtc gataagactg gtgatctgca aataaaaatc ttgctcacgt
      781 cttctgattt ttttgccaaa catgctcatg gttattgccc tttcgcagga ttagctgcag
      841 cggccgagag accaccaatg atgaagtcgg gaaacagtac gtccatcaac atcactttgc
      901 cgttgcgttc gtcggttact tttatttcgc cgtctttttt ggggtctagg ttacgagttg
      961 catccatgag aaacaccaaa tcggcgtagg ccatgtcgat gtcagagtta aagtgaatag
     1021 tgaactcttt agggcgttcc ttttttattt taactaaagt gtcgtgcaat gttgtgagat
     1081 cgggtttgcc cgcaatgtga ggaacgctgc gtttgccgct caaacttccc gccccaactg
     1141 cggaaatatc aatgccgttt ttgcgataga cgagatcaag tttgaaggtg gggtccttgc
     1201 gttgagcttc cggggcggaa gctggaacag cgagaggcac tttggcgtcg agtccaacaa
     1261 gagcgacgta agtagtggat aacaacataa aacaaatcaa cgtcacaaag cagtcgataa
     1321 tcggcgttaa attcagctca aaatcgtgag ctgtgtgcgc tcgcttgtgg aacatggctt
     1381 atgcagctcc tttaatgccg gacccattgc ctgtttctac ggtgtcaaaa ctcacgttcg
     1441 aaaaatcatc ttctccggca tagacgcggt tatgtaaaag cgagagtgtg cgaccggcac
     1501 ctttttcgat ttcgtctaat atcttgttag agcgaccgct gagtaagcta aaagcgatca
     1561 tcgccggaat cgcgaccact agacctaagc cagtggcgtt catcgatacg gagatacctt
     1621 ccgccaagag gctttgttta gttgcggcgt ctgcgctggc cactgcttta aatgactgaa
     1681 tgagtcccgc gatcgtgccg agtagtccca aaagtgtggc gacgtttgca atcatagcta
     1741 aatacccgat gcgagcgctg agcaagctgt tggcatcgat caccgctact tcgagagatg
     1801 tttttatttc ttcctggttg cgcgacgctt tcagcaatgc ggctttcacc acacgcggta
     1861 aaagtttctg ctcgttggca gcgcagaggt ccagtgcgcc ttgaaggttg cccttttgta
     1921 gaaggccttc gatctggctc atgaacaatt tgctgtcgat cgagtattga aaaaacagct
     1981 tggaagtgcg ctccgcgata agcgctgctg ctacgacccc ggtggctgct attggaagta
     2041 aatgtagaat catatgttcc ccctgtgatt acctaataat atcgtttggt gtgtaaaaga
     2101 cgttgattgg aatattgggt ccgtctggaa tggccgaacc gtgaaacacg atactgttga
     2161 tgctggcctc gtaagtccag ccgttggttg ccgactgcgc caagtattcg ccgtttttaa
     2221 acactgcaat tgttccagga tctggtgtgc gcgaaaggcg aatggcagta gaagcttcta
     2281 gaattttggt tgcgacatta agtaatccag ccgaaaaatc tggagcgcaa atgctggcaa
     2341 cctggccgcc ggtattgtta gcgagttcaa tgtatttcga cccgtattca ctggctggcc
     2401 cgagagcaat acagtccggg tcgtttacgt cgtccaccac catggctgat gcgttccaac
     2461 ttcgaatcgt tgaaccactg gccaaagtga agggggtttt tatgccgtct agaaaggtag
     2521 ttaatcccga aactgttgct gcggtgttgt cgtcggcatc gctaaaaaag atcacggcga
     2581 ggtaggcgtc ttcccgaaag aagccgctat tgcagttcga aagattgggt tctgacaacg
     2641 cgagttccac tgcgtcgaga ccgtgagcgt ctgatgatcc gaaaaatccg acgttagcat
     2701 tgtcggagac ttcagtagcg ggattgggag tcgaagcctc cactacggtc ggcgagccca
     2761 cgaaacaagc cgcctggcca ctcaagtttg gatcgttcgg tgcgagcgga tcaactgcgc
     2821 gagtgtcggt cgtggtgatt gcgatgcgat agtcaaaatt cttacttacg aaactgttca
     2881 tgaaagagtt gatgctgttg cgtagactag tttgtttggg gcccattgtt cccgagttgt
     2941 cgataatcca aagaatatct actttgttgt ttgccactcc cgaagttacg gtggagtcgg
     3001 agcgcccaag tactgcaata cgggggtcgc gaaatccgca actggtaagg gtactgccga
     3061 gggctgccag gccaagaaac agtagggcgg cgtatccgtg atgggcgatc aggccagtgg
     3121 tcgatcgtat tttgttatac tgcatggccg gttcctcaac ttgcttgtta aaataattta
     3181 acagttacgg gaatagttta ctttttaagt cggttgttat caagtaatta atgcatcgca
     3241 taaatagcga gaatctttag ttttttttgc tatcccgttt ctatttcaat aggatcgacg
     3301 ttgaggggtc gggcgaaacc ggggccctta agattgtc
//
LOCUS       DAOMXO010000210         3327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15921_length_3327_cov_4.430318, whole genome shotgun sequence.
ACCESSION   DAOMXO010000210 DAOMXO010000000
VERSION     DAOMXO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15921_length_3327_cov_4.430318"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLH57_05420"
     CDS             <1..274
                     /locus_tag="PLH57_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRK02085.1"
                     /translation="FDPPEKVTFWMRNTHIPLDLALFNPKGRLTDVRHMPVEPDPTDP
                     KRTYNSSREVLFALETPYDSLTAVEKEPVLMCLDSAKIKLSRETTR"
     gene            330..590
                     /gene="grxC"
                     /locus_tag="PLH57_05425"
     CDS             330..590
                     /gene="grxC"
                     /locus_tag="PLH57_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HRK02086.1"
                     /translation="MKTVTIYTMEYCPYCENAKRLLASKSIPYKEIKVSLDDEPEWDR
                     LEKLTGYKTMPQIFIDEHFVGGYRELTAIEQSGELDKLLSSS"
     gene            587..1387
                     /locus_tag="PLH57_05430"
     CDS             587..1387
                     /locus_tag="PLH57_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRK02087.1"
                     /translation="MNLSAIRKQIPEADTVVFVHGLLGSCRNLFRLYDAVHKEGFSVF
                     AYDQRGHGHSPHGSPESYTVQQLAADAIELMKQQGIQKAHFVGHSLGGRVSLLVAALA
                     PTLVQSLTLLDVGPRVSRRGVDNVRDIILPMPPSFANREEADQFLSRYQDAGLQQFLR
                     SNLRDRDGILAWIFDLHGIKTRLLEALEENYESYYATVQCPIHLVRGALSSHLSAEEL
                     QYLQKNNSKAFATTIQKAGHWVHVDNFEDTAAAVIQFLQKHSSRSTNS"
     gene            1384..2673
                     /locus_tag="PLH57_05435"
     CDS             1384..2673
                     /locus_tag="PLH57_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HRK02088.1"
                     /translation="MNEIVAALTLLLSLEQSGSKQVLHLEPPAQHHLNIEAPNSVKDK
                     TGKISKKLDATDSKLRFELSPALNAPFTVTAYLCDNAKTYCVKKTHQIDTLSSLPPDQ
                     HTPPQKNGVRKQGSSPLSANEDFLKNDPKTAFATAKKHNRPILIDFFGIWCPPCNLLD
                     AEVFAHPDFKKTYASFVKLKMDADSPLSWELKSKYKVTGYPTIIFADTSGTELGRMIG
                     YYPLSDFLTVHTAISSGMPFPQIQAELSLPHDALMSKAEEAEKNGNIDSARNLWLAAS
                     ALLKKTLNERSKLKHVDAKSLERSISTDLAYSLYRGGKVKEAESLYRKMQRLYPQEFT
                     FSYYYARMLNDLGRSKDAEAPARLAVKYSYGDNHLRSVDALAVALHKQGKSEEANNLI
                     EATIKATTLPEDSALRTHRYLNKLRETQASFAKASTQ"
     gene            complement(2667..>3327)
                     /locus_tag="PLH57_05440"
     CDS             complement(2667..>3327)
                     /locus_tag="PLH57_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02089.1"
                     /translation="TGAQRKKAKIIYENVAKNLRTEKRKLIKMKAANEGPDLVNPQAK
                     LVAKLQKASNRLEENLAPRKVLRKRKIDLGVARVTLKSLHKAKERIGKYFTHIRSNKG
                     SSRAWNRVKIRSYWYATNVAGQFTALFMLPLYLIGLNYLVGDQKNPGPLRDFFSSLGA
                     AASWLWSMGGDMLEDALDRIHGDQAINDGEQSDQERSDNQALIERIEEVRLEAELAEI
                     Y"
BASE COUNT          918 a          868 c          677 g          864 t
ORIGIN      
        1 ttttgacccg ccagaaaaag ttacattttg gatgcgaaat actcatattc ccctcgatct
       61 tgctcttttc aaccccaaag gtcgactcac cgatgtgcgg cacatgccag tcgaaccaga
      121 tccgacagat cccaagcgga cctataactc ctcccgagaa gtactattcg ctcttgaaac
      181 cccatatgat tcgctcacgg cggttgaaaa ggaaccggta ctaatgtgtc tcgatagcgc
      241 gaaaatcaaa ttatcccgag aaaccaccag atagtcccaa aacacagccg gttgcgaaaa
      301 tttcgcactt cgaatagctt gaggggtgca tgaaaacagt cacgatatac acgatggaat
      361 actgccctta ttgtgaaaac gccaaacgcc tcttggcttc taagtcgatt ccttacaagg
      421 agatcaaagt ctctttggac gacgaaccag aatgggatcg attagaaaag ttaacgggat
      481 acaaaacaat gccccaaata tttatcgatg aacacttcgt cgggggctac agggaactta
      541 ccgcgattga gcagtccggc gaactcgaca aattgctttc ttcctcatga acttatctgc
      601 catccgaaaa cagattcctg aagctgacac cgttgttttt gttcacggtc ttttaggttc
      661 ctgtcgcaat ctctttcgac tttacgatgc tgtacacaaa gaaggatttt ctgtttttgc
      721 gtacgaccag cgcgggcacg gacacagtcc tcatggatca ccagaaagct acaccgttca
      781 acagttggcc gccgacgcca ttgaactcat gaagcaacag ggcattcaaa aagctcactt
      841 cgtcggacac agtctcggcg gaagagtaag cctattggtt gctgctctcg caccaaccct
      901 cgtgcaaagc ctcacactgc ttgacgtggg gccgcgcgta tcacgccggg gtgtcgacaa
      961 cgttcgtgat attattctcc ccatgccccc atcttttgca aatcgcgaag aagcagatca
     1021 gtttttaagt cgttaccaag acgctgggct tcaacaattc ttgcggtcca atttgcgaga
     1081 ccgagacggt attctagctt ggatttttga tctccatggc atcaagaccc gtctattgga
     1141 agcccttgaa gaaaactatg agtcctatta cgcgaccgtt caatgcccta ttcacttagt
     1201 tcgcggcgcg ttgtcgtccc atttgagtgc cgaagagttg cagtacctcc aaaaaaacaa
     1261 cagtaaagcc tttgccacca cgattcaaaa ggccggccac tgggtgcatg tcgataattt
     1321 cgaggacact gcggccgctg taattcagtt tctgcaaaaa cactcttcga ggtctacaaa
     1381 ctcatgaacg aaatcgtcgc tgcacttacg ctgctattaa gtttagagca atctggttcc
     1441 aaacaagtac tgcacctgga acctcctgca cagcaccacc tcaatattga ggcccccaac
     1501 tctgtaaaag acaaaacagg aaaaatctcg aaaaagttag atgccaccga ttcgaaactt
     1561 cgcttcgaac tttcgccagc tttgaatgca ccctttaccg taaccgctta tctctgcgac
     1621 aacgccaaaa cttattgcgt aaaaaaaacc catcaaattg atacgctttc aagtcttccc
     1681 cctgatcaac atacgccccc ccaaaaaaat ggggttcgca aacaaggcag ctcgccgcta
     1741 tcagccaatg aagacttcct aaaaaacgat ccaaaaaccg cattcgccac tgcgaagaaa
     1801 cacaaccgtc cgattctaat cgattttttt ggtatctggt gtccaccttg taatttactt
     1861 gatgccgaag tttttgctca ccctgacttt aagaagacat acgcttcttt tgtaaaatta
     1921 aaaatggatg cagactcacc gctgtcgtgg gaactgaaat ctaaatataa agtaaccggt
     1981 taccctacaa tcatatttgc tgacacgtct ggaactgagc ttggtcgaat gattgggtat
     2041 taccctctaa gcgatttttt aactgtccac acagccattt cgtctggcat gccgttccct
     2101 caaatccagg cagagctttc actgccccac gatgccctca tgagtaaagc ggaagaggct
     2161 gaaaaaaatg gcaatatcga ttctgccaga aacctctggc tagccgcctc tgcactgctt
     2221 aaaaaaacac ttaacgaacg atcgaaactt aaacacgtcg acgccaaatc gcttgaacgc
     2281 agtattagta ctgacttggc atattctctc tacaggggcg gcaaggtaaa ggaagctgag
     2341 tcgttgtatc ggaaaatgca acgactctat cctcaggaat ttaccttctc ttattactac
     2401 gccagaatgt taaatgattt aggccgatca aaagatgcag aggctcctgc ccgactcgcc
     2461 gtgaagtatt cttatggtga taatcatttg cgctcagttg atgcattggc cgtggcactt
     2521 cataaacaag gcaaatctga agaagcaaac aatctgatag aagccacgat taaggcgacc
     2581 accttacccg aagactccgc cttacgaacg caccgctacc tcaataagct tcgagaaact
     2641 caggcaagtt ttgcaaaggc atctactcag tagatctcgg ccaactctgc ttcaagcctt
     2701 acttcttcaa tgcgctcgat cagggcctga ttatctgacc gctcctgatc cgattgttcg
     2761 ccatcattta tagcttgatc accgtgaatt ctgtcgagag cgtcttcgag catatcgccg
     2821 cccattgacc acaaccaact cgcggcggca ccaagggaac taaaaaagtc ccgtaaagga
     2881 cccggatttt tttgatcacc aaccaggtaa ttcaatccta tcaaataaag tggcaacatg
     2941 aacaaagcgg taaactggcc agcgacattt gtggcgtacc aataggatct gatctttacg
     3001 cggttccatg cgcgactaga acctttattc gatcgaatat gtgtaaaata ttttccaatt
     3061 cgctctttag ctttgtgcaa ggactttaaa gtaactcgcg ccactcccag atcaattttt
     3121 ctttttctca aaaccttgcg gggtgccaaa ttttcctcaa gacgattgga agctttctgc
     3181 agtttcgcga ccaacttcgc ctgtggatta actaggtcgg ggccttcgtt cgccgctttc
     3241 attttaatta attttctttt ttcggttctc agatttttcg ctacgttttc gtagataatt
     3301 tttgcttttt tccgttgggc gcctgtg
//
LOCUS       DAOMXO010000211         3322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_15959_length_3322_cov_4.259565, whole genome shotgun sequence.
ACCESSION   DAOMXO010000211 DAOMXO010000000
VERSION     DAOMXO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15959_length_3322_cov_4.259565"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLH57_05445"
     CDS             <1..868
                     /locus_tag="PLH57_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02090.1"
                     /translation="YSGEVGVESEDLFKLSPNFQRKLRGLMNAIEERDYELQRPVTYV
                     SASKFSMEERSFERLSKLVPDMVSVKADLSARSRALHERLIITDPRTIVRLVELVQAL
                     AVAQTGKIVALPLPMKGLSNYTVDAVAQLDKFRKGKDPLRIQLGVGYEVYDYQLGQVV
                     FYDPTLFWSLAATEEDSHEAISMFLQSLIGLAEVKVPCSSSDPRVNVVHYVAKEDSAR
                     RLVFLINPTHNEIHTRLNFPNSVQVAPLATGNLGDSPQPIGGQSFELRVPALGVLPMQ
                     IFDIPSVEGGTA"
     gene            868..1668
                     /locus_tag="PLH57_05450"
     CDS             868..1668
                     /locus_tag="PLH57_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02091.1"
                     /translation="MDLSHDWKSLNEVLFCQKGLAKHAPTLVALEDGKKIFDAVLSTG
                     QPYSDRLAARDTIGELSLKYGVEQTVSFAKKELDQIVADAASLGTNYFQQLEYVRSKV
                     YKHVNKPSEIRISRDHFILRFFSGKMASLLPRRFNVLVFLDQRPSFSSTLLAFEHRAI
                     LLTFDRGKLDQFFEPDFSSLHEQRLPEWLLDPVAIGKYLESRYILPCYGIFMLQSDWD
                     QCVTGDDPWSVVISALDDHRAAIYPRTGLVKTLIAFQRISGYLSGFRR"
     gene            1724..2815
                     /gene="rlmN"
                     /locus_tag="PLH57_05455"
     CDS             1724..2815
                     /gene="rlmN"
                     /locus_tag="PLH57_05455"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRK02092.1"
                     /translation="MGCEVSAASVPPKRHLCDLRMGDLALELAGLGLAEKKARYHAKQ
                     IFNWVYQRKVTDFEAMTDLSKLVRSHLKENFDLTPLGKVFEQKSVDGTQKFLFQLSDG
                     KTIESVLIPAQDRLTICISSQVGCAMGCKFCNTASQGLMRNLEVHEITRQVLELSRVA
                     NITNIVYMGMGEPLHNFENVCRSVEILLSQEGLNFSKRKITISTSGLVPQIRKMNERL
                     VGPNRVNLAISLNGSYDSQRESVMPINRAFPIEELMQACRDFELEPHRRITFEYVMLG
                     GLNDNLEDAERVMKLVEGIPSKINLIPYNPHPASPYDRPKEEKVEAFHRYLLDRNVSA
                     MIRHSRGRDILAACGQLKSKFDEVVSVAQ"
     gene            2790..>3322
                     /locus_tag="PLH57_05460"
     CDS             2790..>3322
                     /locus_tag="PLH57_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02093.1"
                     /translation="MKLYQSLSKIAVFVFWVCPAIAVASTDDAVHFTANCAKLISEHW
                     NSSSAEDKHVFEEEMARISDGLGRNRVEWSRAFLNVYSDSDRGHEGPRGISTILGEVG
                     DDSRLRRVAVFDHPSAEKRFQYIVPNPVIGRIFQIALHPDGRAAVILVRPTNADSWFS
                     KETTLIVSDKNPSLVPL"
BASE COUNT          826 a          787 c          808 g          901 t
ORIGIN      
        1 ttattccgga gaagtgggcg tagagtccga ggatttattt aaactttccc cgaattttca
       61 gcgtaaactg cgcggcttga tgaatgccat cgaagagcgc gactacgaat tgcaacgacc
      121 agtcacttac gtttccgcca gcaaattttc tatggaagaa cgttctttcg aaagactgtc
      181 gaaactagtg cccgatatgg tttcggttaa ggccgacctc agtgcgcggt cacgggcatt
      241 gcatgaaagg ctgatcatta ccgacccaag aaccattgtt cgattggtcg aactggtgca
      301 ggctttggct gttgcacaaa ccggtaagat tgtcgctttg ccactgccca tgaaggggct
      361 ttccaattat acggtcgacg ccgtggctca actcgataaa tttcgaaagg gcaaagaccc
      421 cctacgaatt caattgggcg ttggatacga ggtctacgac tatcagttgg ggcaagtcgt
      481 attttatgat cctacgttgt tttggtcgct tgcggctacg gaagaggact cgcatgaagc
      541 gatatccatg tttttgcaat cgctaattgg cctggcagaa gtaaaagtgc catgtagttc
      601 ctcggaccca cgagtgaatg tggttcatta tgtggcaaaa gaagactcag ctcggagatt
      661 ggtgttttta attaacccta cacataacga aattcacaca cggttgaatt ttccgaattc
      721 cgtacaagtg gcacctctgg ccaccggtaa tcttggtgat tcgccacagc cgattggtgg
      781 ccagtcattt gaacttcgag ttccagcatt gggcgtacta cctatgcaga tattcgacat
      841 tccttcggtc gaagggggaa cggcctaatg gatctttccc acgattggaa atcactaaac
      901 gaagttcttt tttgtcaaaa aggtttagct aaacacgcgc ccacgctcgt agctttagaa
      961 gacggtaaga aaattttcga tgcggtgttg tctactgggc agccctattc ggatcgactg
     1021 gctgctcgag atactattgg agagctttcg ctcaaatacg gcgtagaaca aaccgtttcc
     1081 tttgcaaaaa aggaacttga tcagatagtc gctgacgctg cgtcgctcgg aaccaattat
     1141 tttcagcaat tggagtacgt tcgttcgaag gtttataaac atgtgaataa gccaagtgaa
     1201 attcgtattt cacgtgatca cttcattctt cgtttttttt ccggaaaaat ggcttcattg
     1261 ttgccacggc gctttaatgt tttggttttt ttagaccagc gcccttcgtt ttcgtccacc
     1321 ttgctcgcct tcgagcatcg ggccatactt ttgacctttg atcgcggaaa gcttgatcag
     1381 tttttcgagc ccgatttttc gagtttgcac gaacaacgac ttcctgaatg gctgctcgac
     1441 cctgttgcca tcggaaagta cctagaaagt cgttacatcc taccgtgtta cggaattttc
     1501 atgctgcagt ccgattggga ccagtgtgtc acgggtgatg acccttggtc ggtcgttatc
     1561 tctgcgctcg acgatcatcg ggctgcgatt tacccgcgca cgggtcttgt taaaacccta
     1621 atcgcttttc agcgaatttc cgggtatctt tcaggttttc gccgctaatc cccacagaaa
     1681 ccccttgcac ccggtctaat gatggggtaa atcgctcggg aatatgggat gtgaagtatc
     1741 ggctgcctca gtaccgccaa aacgtcacct ttgcgacctg cgcatgggag atctcgcgct
     1801 cgagctcgcc ggactaggat tggccgaaaa aaaagctcgc taccacgcta aacagatctt
     1861 caactgggtg taccaacgca aagttaccga ctttgaggcc atgactgatc tttcaaagct
     1921 cgttcgttcg catttgaaag agaacttcga cttaacgccc ctagggaaag tgtttgagca
     1981 aaagagtgtc gatgggactc agaagtttct ttttcagctg agcgacggaa aaacgatcga
     2041 aagcgtgttg attccggcac aagaccgcct caccatttgc attagttccc aggttggctg
     2101 cgcgatgggc tgcaagtttt gtaatacggc ctcccaggga ttgatgcgca atctcgaagt
     2161 acatgaaatc acccgccaag tattagaact ttcgcgcgtc gctaacatta ctaatatcgt
     2221 ttacatgggc atgggcgagc cattgcacaa cttcgaaaat gtctgccgat cggttgaaat
     2281 tcttctttcg caggaagggt taaatttttc aaaacggaaa attacgattt ctacgtctgg
     2341 tctggtgccg caaattcgaa aaatgaatga acgactggtg gggcctaatc gtgttaatct
     2401 cgcaatttca ctcaatggtt cctacgattc gcagcgtgaa tcggtcatgc caatcaatcg
     2461 tgcattcccc atcgaagagc tcatgcaagc ctgccgcgac ttcgagctcg aaccgcaccg
     2521 ccgaattacc tttgaatacg tgatgcttgg cggtctaaac gacaatctcg aagacgctga
     2581 gcgagtcatg aagttagtcg aggggattcc ctcgaaaatc aatctgattc cctacaaccc
     2641 gcatcctgca agtccctacg accgtcctaa agaagaaaaa gtggaagctt ttcaccgcta
     2701 tctattagat cgaaatgtat ctgcaatgat tcgtcacagc cgaggccgcg atattttggc
     2761 cgcttgtggt cagttaaaga gcaaattcga tgaagttgta tcagtcgctc agtaagattg
     2821 cagtttttgt tttctgggtg tgcccggcaa ttgcggtagc ctccacagat gatgctgttc
     2881 attttactgc aaattgcgcg aaattaattt cggagcattg gaatagttct tccgccgaag
     2941 acaaacacgt tttcgaagag gaaatggccc gtatttcaga cgggcttggt cgaaatcgag
     3001 tcgaatggtc gcgagcgttt ttaaacgtct acagtgactc tgatcgaggt catgagggac
     3061 ctagaggtat atcgaccatt ctaggagaag ttggcgacga cagccgattg cgccgggtag
     3121 cagttttcga tcacccttcg gccgaaaaac gattccaata tattgttccg aatccagtaa
     3181 ttggacggat atttcagata gcgttgcatc ccgacgggcg agccgctgtt attttggttc
     3241 gccctacgaa cgccgattcc tggtttagca aagaaactac cttgatcgtc agtgataaga
     3301 acccaagttt ggttccactg ca
//
LOCUS       DAOMXO010000212         3262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_16374_length_3262_cov_4.582476, whole genome shotgun sequence.
ACCESSION   DAOMXO010000212 DAOMXO010000000
VERSION     DAOMXO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16374_length_3262_cov_4.582476"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..781
                     /locus_tag="PLH57_05465"
     CDS             44..781
                     /locus_tag="PLH57_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRK02094.1"
                     /translation="MFVRIVPVLFFFVTITFSHAGSDENRTTNTKKNHPKSATEKEIG
                     GNKIISEMQSKYRTVRTLSANFVQNQFSAMDAPDHKIIPSVGNIFIERDPKLDLLKFR
                     WQTISPNPALAVSNGRKFWAYTPPFRDGENGQVQIRKASDMKSSTLADLLLANRPLQK
                     SFDIEPLDCKNQNHACFSLVPKKASGDLDRAELHIERRTKLVYKVNLINIVGNRTELE
                     LKNIQLNPKLGKALFEFKVPPKTEVIK"
     gene            783..1268
                     /locus_tag="PLH57_05470"
     CDS             783..1268
                     /locus_tag="PLH57_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRK02095.1"
                     /translation="MPSFDIVSKLDLQEVDNAVNIAKKEIANRFDFKGSKASIELTKE
                     EITLIADDEPKLLQVKDILESKLTKRGVSLLVLDYQKLEDATLGTVRQKAKLRQGVDK
                     ENGKKIIQLIKDTKLKVQAQIMDDQVRVTGKKIDDLQEVMGVLRTSQVPLPLQYVNMR
                     S"
     gene            1288..2670
                     /gene="rimO"
                     /locus_tag="PLH57_05475"
     CDS             1288..2670
                     /gene="rimO"
                     /locus_tag="PLH57_05475"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRK02096.1"
                     /translation="MEYQAGRKIESGVERDKNVHFVSLGCPKNLVDSQVMLGLLKSDQ
                     YGVVSEPERADVIVVNTCSFIESSKVESIDTILELAEYKDTGKCKTLVVAGCLPQRYK
                     SQLLKELPEVDILIGTGEYHRIAELVDWAQKGELPERAYVNKPAFIHTEAHPRLQTGQ
                     FFSAYLKISEGCNRRCSFCIIPHLRGNTRSRTVESLVEEATKLALCGVKELNLVAQDL
                     TEYGMEHRYQQMTLEKLLPALCKIEGIEWVRLFYAYPDQFSDELIDIMATEPKVVKYL
                     DMPIQHTNDRILQLMNRKFTRQEILDFVARLRAKMPNIVLRTSIIAGFPSETDAEHEQ
                     LLEDLRVLQFDHLGAFSYSKEEGTKAALLGEQIPARTKAKRQRAIRALHQKISSEKTA
                     GLVGQEFSVLIEGEHDETELLLKGRHYGQAPEIDGQVIINDGSASPGDLVRVRITEAM
                     PYDLVGTIIQ"
     gene            complement(2671..>3262)
                     /gene="mltG"
                     /locus_tag="PLH57_05480"
     CDS             complement(2671..>3262)
                     /gene="mltG"
                     /locus_tag="PLH57_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRK02097.1"
                     /translation="YLFPDTYFITREADVKSVLSNMVHKMASIWTPQWVSRTQELKMS
                     LHEVMTLASIVEKETGADFERPLVASVFHNRLKKRMRLQTDPTVIYGIKNYNGNITRK
                     DLETPTPYNTYVIYGLPPGPIANPGRAAVEATLYPKESPFLYFASKNDGTHVFSATYE
                     DHQNAVRKFLMDPKARAGKSWRDLNKKTSPPAPALE"
BASE COUNT          974 a          724 c          738 g          826 t
ORIGIN      
        1 caaaaaaaaa attctatgag attcattttt ttggaggtcc cacatgtttg tccgaattgt
       61 ccccgttctg ttttttttcg tcacgatcac cttttctcac gcaggttctg acgaaaatcg
      121 cactactaat acaaaaaaaa atcacccgaa atcagctaca gaaaaggaaa taggaggaaa
      181 caaaataatt tccgaaatgc aatcaaagta ccgcacagtg agaactttga gcgcaaattt
      241 tgttcaaaat cagttctcgg cgatggacgc acccgaccat aaaataattc cgtcggtagg
      301 caacattttc atagaaaggg acccaaaact cgatttattg aagtttcggt ggcaaacaat
      361 cagtccaaat ccggctcttg ccgtttcaaa tggaagaaaa ttttgggcat atacaccgcc
      421 atttcgcgac ggtgaaaacg ggcaagttca aatacgtaaa gcgagtgata tgaagtcgag
      481 cacattggcc gacctattgc tcgcgaatcg gccactacaa aagtcctttg atatagagcc
      541 cttagactgc aaaaatcaaa atcatgcgtg tttttcgctt gttccgaaaa aggcttctgg
      601 agacctggat cgggcggaac tccacattga aaggcggact aaattagtat ataaggtgaa
      661 cctcattaat atagtcggca accgtacaga actcgaattg aagaatattc agctaaatcc
      721 aaaattggga aaagcactct ttgaattcaa ggttccgcct aaaacagagg tgattaagta
      781 gcatgccttc gttcgacatt gtttcgaaac tggatcttca agaagtcgac aacgccgtta
      841 atatagcgaa aaaagaaatc gccaatcggt ttgatttcaa gggcagcaag gcgtcaattg
      901 aactgactaa agaggaaatc acccttattg ccgacgacga accgaaatta ctccaagtga
      961 aagatatatt agaatcgaaa ctcaccaagc gaggcgtatc gcttcttgtt ctcgattatc
     1021 agaagcttga agacgctact ctgggcacag ttcgccagaa agccaaactt cgacaaggcg
     1081 tcgacaaaga aaacggcaaa aagatcattc aattgataaa agacaccaag ttgaaggtac
     1141 aggcgcaaat aatggacgac caggtacgag tgactggcaa aaaaatcgac gaccttcaag
     1201 aagtgatggg tgttttgcgc acttcacagg ttccgctgcc gcttcaatac gttaacatga
     1261 gatcttaaca gcttacggag tagtaaaatg gagtaccaag caggccgcaa gatcgaatcg
     1321 ggcgttgagc gcgacaaaaa cgtgcacttc gtaagccttg ggtgtccaaa aaatctggtg
     1381 gattcgcaag tcatgctggg tcttctaaag tcggaccaat acggcgtggt ctcggaacca
     1441 gagcgcgccg acgttattgt agtaaatact tgcagtttta ttgagtcctc caaggtcgaa
     1501 tcaatcgaca cgatcttaga gcttgccgaa tataaagaca ctgggaagtg taaaacgctc
     1561 gttgtagcgg gttgtttgcc ccaacgctac aaaagccagc ttttaaagga actccccgag
     1621 gttgatattc taattggcac cggtgagtat catcgcatcg ccgaactcgt ggattgggcc
     1681 caaaaaggcg aacttcctga gcgagcctac gtgaataaac ctgcttttat tcataccgaa
     1741 gcgcacccga gactgcagac gggtcagttc ttctctgctt atttgaaaat ttccgaaggc
     1801 tgcaatcgta gatgcagttt ttgtattatc cctcacctgc gaggcaacac tcgttcgcgc
     1861 actgtggagt ccctggtcga agaagccacc aaactagcgc tttgcggagt aaaagaactc
     1921 aatttggtag cccaggattt aaccgaatac ggaatggaac accgttatca acaaatgact
     1981 ctagaaaaac ttcttcctgc attgtgtaag atcgaaggca tcgagtgggt acgtttgttt
     2041 tatgcctatc ccgatcaatt ttccgacgaa ttaatcgaca tcatggcaac tgagcctaaa
     2101 gtagtgaagt acttagatat gccgattcaa cacaccaatg atcgcattct tcaactcatg
     2161 aaccgcaagt ttactcgtca ggaaatttta gacttcgtgg cacgtttacg ggcaaaaatg
     2221 ccgaacattg tacttcgaac ctctataata gcagggtttc catcggaaac tgatgccgag
     2281 catgagcaat tactagaaga cttaagagtt ctgcaatttg accatctcgg ggcgttttcc
     2341 tattcaaaag aagaaggtac taaggccgcc ctacttggag agcaaattcc cgctcgaaca
     2401 aaagccaagc ggcaacgagc gatacgggca ttgcaccaaa aaatttcgtc cgaaaaaaca
     2461 gctgggcttg taggccagga attttcagtt ttgatcgaag gcgagcacga cgaaacagaa
     2521 ttacttttaa aaggccggca ttatggacag gccccagaaa ttgatggcca agtaattatc
     2581 aatgacggct cagcatcccc cggtgattta gtgcgagtgc gcattactga ggcgatgccc
     2641 tacgatctag tcggtaccat aattcaataa ctactcgaga gccggcgctg gtggagatgt
     2701 tttcttgtta agatcgcgcc agctcttgcc cgctctggct ttggggtcca taagaaactt
     2761 gcgtacggca ttttgatgat cttcataggt cgcgctaaaa acatgagttc cgtcgttttt
     2821 ggaagcaaag taaaggaagg gactttcctt tggatacaac gttgcctcaa ctgcggctcg
     2881 gccaggatta gcgattggac ccggcggtaa gccataaatt acataagtgt tgtaaggagt
     2941 tggtgtttct aagtcttttc gtgtgatgtt gccgttatag tttttaattc cgtaaatcac
     3001 cgttggatcg gtttgcagtc gcattctctt ttttaaacga ttgtgaaaaa ctgaagccac
     3061 gagtggacgc tcgaaatcag ctccagtttc cttttctacg atactcgcca acgtcatgac
     3121 ctcgtggagt gacattttta gttcctgcgt tctcgaaacc cactgaggtg tccaaatact
     3181 tgccatcttg tgtaccatgt tactaagtac cgacttaacg tcggcttcac gggtaataaa
     3241 ataggtatct ggaaacaaat at
//
LOCUS       DAOMXO010000213         3250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_16465_length_3250_cov_4.833803, whole genome shotgun sequence.
ACCESSION   DAOMXO010000213 DAOMXO010000000
VERSION     DAOMXO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16465_length_3250_cov_4.833803"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLH57_05485"
     CDS             <1..392
                     /locus_tag="PLH57_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRK02098.1"
                     /translation="TTLRALLSTSSNLITVFGCGGDRDPSKRPIMGQIATTHSDKVWI
                     TSDNPRTEDPEKIIHEVMVGAKSVSSAAKIHTEANRRLAIEAALNTLKAGDILLVAGK
                     GHETYQIIGTQKLPFDDRVVAREYLNR"
     gene            413..1876
                     /gene="murF"
                     /locus_tag="PLH57_05490"
     CDS             413..1876
                     /gene="murF"
                     /locus_tag="PLH57_05490"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRK02099.1"
                     /translation="MKTLSLKFHEIRTAVDGRILQEGASDQIYSAFISDSRKIEKDCI
                     FLCIAGNKVDGHDFASQAADSGAALIIGQNETKLRALENNHPRVGLMLVKDALRATQA
                     LATYYRSKFEIPVIGLTGSSGKTTTKDLIASIANVSNKDHTLITEGTLNNHWGVPQMI
                     LRLNQSHRAAVLEMGMSDFGEIQSLAAIGQPTIGYITAIGPAHLEKLGSVEGVLRAKR
                     ELFDWINLHQTKGQLLFNIDDPLLAKLHSEIKTQCRLSPLTLSLQSEADFQLLRCSQL
                     DEDGHFGWHYEIKTPNGTIEGTLPLPGKHNLTNALAAVALTLVSGIAGKNDIKAGLAK
                     PKISSLRSELFRSKHGATIYNDSYNANPTSVGALFDTAHLILENFQGRYDEIIAAVGD
                     MLELGPTANELHRDMGRKAAESGVTTLLALGHHREHWADGFQNTGRKVECRTFQDHES
                     LYRDLQHRLELKPRALVLVKGSRGAKMDLVASQLKAK"
     gene            1895..2971
                     /gene="mraY"
                     /locus_tag="PLH57_05495"
     CDS             1895..2971
                     /gene="mraY"
                     /locus_tag="PLH57_05495"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRK02100.1"
                     /translation="MFPWLLVSLSTEVTFLNVFKYITFRTFGAAITALVLSMIVGPWY
                     IAMMQRRQIGQSIRELGPQSHQSKKGTPTMGGGLILFCITASTMLWADLSNPLIWIVL
                     FVTLATGIVGWADDYLKVIKKNSKGVSGRAKIFWQSLIAVTAASLLYFKFGMSPELRF
                     PFFKDLVINLGPLYIPFAVLVIVGASNAVNLTDGLDGLAIGPIIISAGTYTLLAYLAG
                     HAKIAEYLQIPHVPLAGELAVFCGAMAAAGLGFLWFNSYPAQIFMGDVGSLPLGAALG
                     SIALVTKNEILLVVIGGIFVLEAVSVMAQVFSFKTTGKRVFRMAPIHHHFELKGWPEP
                     KVIVRFWIISILLAIIALSTLKLR"
     gene            2992..>3250
                     /locus_tag="PLH57_05500"
     CDS             2992..>3250
                     /locus_tag="PLH57_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRK02101.1"
                     /translation="MKIKGKKFLVVGLGKTGVSATRFILAQGGKVTITDSRTRMELAE
                     SIKLLGDLKVEMDLGKHNPKLFTQNDVIVLSPGVPGNIEGLS"
BASE COUNT          856 a          796 c          771 g          827 t
ORIGIN      
        1 taactacgct cagggcactt ctctcaacga gtagtaatct aattacagta tttggttgcg
       61 gcggagatcg agaccccagc aaacgaccaa tcatgggcca aattgccact actcattccg
      121 ataaagtctg gatcacctcc gataatccgc gcacggaaga cccagaaaag atcattcatg
      181 aggtaatggt cggcgcaaag agcgtgtctt ctgcggccaa aattcatact gaggcaaatc
      241 gaagactcgc catcgaagcc gccctgaaca cactaaaggc tggggacatt ctccttgtcg
      301 caggcaaggg ccatgaaact taccaaatca ttggcaccca gaaacttccc tttgacgatc
      361 gcgtggttgc gcgggaatat ttgaaccgtt gagcacctcg aaagaaaact ctatgaagac
      421 gctgtcactt aaatttcacg aaattcgcac cgcggtggat ggaagaattc ttcaagaggg
      481 ggcttcagat cagatttatt cggcctttat ttctgactcg agaaaaattg aaaaggactg
      541 catttttctc tgcatcgccg gaaacaaggt tgacggtcat gatttcgcaa gccaagccgc
      601 ggattccggt gcggcgctaa taatcggcca aaatgaaacg aagcttcgag cacttgagaa
      661 caaccatccg cgagttggac ttatgttggt caaagacgct ttgcgcgcca cccaggcgct
      721 cgcaacctat taccgatcaa aatttgagat accagtaatt ggtttaactg gttcttctgg
      781 aaaaactact accaaagacc taattgcatc gatcgccaat gtctcaaaca aagaccacac
      841 actcataacc gagggtaccc ttaataatca ttggggcgtg cctcaaatga tcctgcgttt
      901 aaaccaaagt caccgggctg ccgtacttga aatgggaatg agcgactttg gagaaattca
      961 atctttggcc gcaatagggc aaccgacgat tggctatata accgcgattg gacccgccca
     1021 cttagaaaaa ttaggcagcg tcgaaggagt tttgcgagca aaacgcgaac ttttcgactg
     1081 gatcaatttg catcaaacca agggtcagtt gctttttaat atcgacgatc cgttgctagc
     1141 taagttgcac agcgaaatca aaacacagtg caggttatca ccactcaccc tttcactcca
     1201 atccgaggcc gattttcaac tactccgatg ctcacagctc gacgaagatg gccactttgg
     1261 ttggcactat gaaataaaga ctcctaatgg aaccatcgaa ggcacgcttc cgcttccggg
     1321 taaacacaac ttaacgaatg ctctggccgc cgtagcatta acactagtga gtggcattgc
     1381 cggaaagaat gacattaaag caggtttggc taaacccaag attagttcac tgcgttccga
     1441 gttatttcgt agcaagcacg gcgcaactat ttacaacgac tcctacaatg ccaacccgac
     1501 gagtgtcggg gcactttttg ataccgctca tttaattttg gaaaattttc agggccgcta
     1561 cgacgaaata atcgctgccg tgggcgatat gcttgagctg gggcctacgg caaatgagct
     1621 gcaccgggat atgggtcgaa aagctgcaga aagtggcgtt actaccctgc tcgcgctcgg
     1681 acaccatcgg gaacactggg cagatgggtt tcaaaacact gggcgaaaag ttgaatgtcg
     1741 aacttttcag gaccacgaat cactctatcg agatcttcaa catcgtttgg aactaaaacc
     1801 acgcgcctta gtgttggtca aaggctccag aggtgcgaaa atggatttgg tagcttctca
     1861 attgaaggca aaatagcgaa aggacccccg ctctatgttt ccatggcttc ttgtatcctt
     1921 gtctaccgag gtcacgtttc ttaacgtctt caaatatata acatttagaa cattcggtgc
     1981 tgcgatcacc gcgcttgtgc tcagcatgat agtggggcct tggtacattg ccatgatgca
     2041 gcgtcggcaa attggtcaaa gtattcgaga actcggtcct caaagccacc aatcgaaaaa
     2101 gggtactcct accatggggg gcggtttgat tttgttttgc attactgcat ccaccatgct
     2161 ttgggccgac cttagcaacc ctctaatctg gatcgtactc ttcgtaacgt tggcaactgg
     2221 gatcgtgggt tgggccgacg attacctaaa agtgatcaaa aaaaattcca agggagtttc
     2281 gggtcgagcc aagatttttt ggcagtcgtt gatagctgta accgccgcct cgctactcta
     2341 tttcaaattt ggaatgtcgc ccgaactgcg ttttcctttt ttcaaggacc tcgtcatcaa
     2401 tttgggtccc ctctatatac cctttgcggt tttggtaatc gttggggcca gcaacgccgt
     2461 caatctcacc gacggactcg acggtctggc tatcgggcca atcattataa gtgcgggcac
     2521 ctacaccctc cttgcttacc tcgcaggaca cgctaagatc gcggaatatt tacagattcc
     2581 tcatgttccc ctggccggag agctagccgt tttctgcggg gccatggccg ctgccggact
     2641 cggatttctt tggttcaact cctatccggc ccaaattttt atgggcgacg ttgggtcttt
     2701 gccgctcggt gccgcgttag gttcgatcgc ccttgtcaca aaaaatgaaa tacttcttgt
     2761 ggtcataggc ggaatttttg tactagaagc tgtctcggtg atggcgcagg tcttttcttt
     2821 taagaccacg ggcaaacgag tctttagaat ggctcctatt catcaccatt tcgaattgaa
     2881 gggatggccc gagcctaaag tgatcgtgcg attttggatt atatcgatcc tgctcgcgat
     2941 cattgcattg tcgactttaa aactgcgtta gttagtagga ggtttcacca ggtgaaaatc
     3001 aagggtaaga aatttttggt cgttggtctt ggaaaaactg gcgtatctgc cactcgtttc
     3061 attctcgctc aaggcggaaa agttacgatc acggactcac gaactagaat ggagctagcc
     3121 gagtctatta aactcctagg tgacctcaaa gttgaaatgg atcttggaaa acacaacccc
     3181 aagctcttca cccagaacga cgtcattgtt ttaagtccgg gagtaccggg aaacattgaa
     3241 gggttgtctg
//
LOCUS       DAOMXO010000214         3249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_16472_length_3249_cov_5.170319, whole genome shotgun sequence.
ACCESSION   DAOMXO010000214 DAOMXO010000000
VERSION     DAOMXO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16472_length_3249_cov_5.170319"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1647)
                     /locus_tag="PLH57_05505"
     CDS             complement(316..1647)
                     /locus_tag="PLH57_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRK02102.1"
                     /translation="MEAENPSFVRALLAEKRWLYFAVGILVFGFTTKLVRLHLPANHY
                     FDEVYHGFTAQVYVQNDPKAYDPWASPPKGFAYEWTHPPLAKVVMAGFLKVFGDRFFW
                     MRFGSVLFGTLSVLFTGLIAYRFLKSIPIAVLAMAFLSLETLHFAQSRIAMNDSYFVF
                     FMLLTFYLYLGLRPKSGIAGSLALGVGFCAGLALSTKWTGFYVIGIIGVDQVIQWWRR
                     RRTEKFFHYLTRAFLFATGAVAAYFAAYIHFFLLGFKWHQFKELQRQMWWYHNNLTAT
                     HSYQSKPWQWLLNVKPVWMHVDYSESGKIGNIYNAGNSVVLLGGLFAVIYLVVQAWRH
                     RRRSKKSVFARRVPLAQPIPMFFLACYGALWIPWMFSPRIMLFYHYLPAVPFLCILLS
                     FALWTLPRGRKAAAAVVCAAGVWFVVFLPHVSGIRVPAKFAEMFYFSVFPR"
     gene            1699..>3249
                     /locus_tag="PLH57_05510"
     CDS             1699..>3249
                     /locus_tag="PLH57_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02103.1"
                     /translation="MNITRWLTKFDLLLLATWGVYFIFLFSRMVTLSPDQGLQVGHEY
                     LWSDWASLHTGMVRIFATKPLSEWFAYHPIYADGKFTYPFLVNAISALLIRIGLSFES
                     AMMAPYYVAFPLLLLGLRRLFTLVLQSQRLAVLAISLFFLSAGLSFYKVLAVYFATGS
                     WDLAFPISSLHSAVPSHSWGANNFLIAMLLPQRAFALGLPVAVWAIYGFFSELLRERP
                     ARYSAVKMAASGVALGILPIVHAHSLIAASIVLFPAGVMALTQKHLRIRALYFAMPAA
                     AVGALFYFIWIAGGIQTRFTQLLIGFTATSVSDWLWFWGFVWGVSLPLALYGTFKATQ
                     KAASIELYLAVAGLTLFILPNFFLFQPTKWDNSKLFLWAFLLLTPAMARALGSLYARN
                     RYLGVGVALIATLTGALELADLQCVSRHQFQILQPQELALAEQVRLGTHSMSRFAAAT
                     THNHFVSTWSARAVLVGYLGWIGNYGFNHQQTYEELREIYRGSPRTLEQIKSHRINYI
                     VFGPAERRE"
BASE COUNT          820 a          885 c          778 g          766 t
ORIGIN      
        1 gcgttgagat ctgcataccc gcctaaataa acaccagcaa ccgtgtcgtc gaattcgtcg
       61 ttggtcgccg ccgaaccgag ctggagacca aagtcaaacc caacgggctt tgcaaatgca
      121 gacgctccac ccaataaact cgctaataaa agaaccaaaa tcgatattct catagttaaa
      181 acacctcgcc ttaatgggtc cgttccatac cacatctctc attaaagtgg tgtcaaatta
      241 tcgggcaaag gttctcgggg agtgctgtta gcactacaac taatcacctc aacctattga
      301 tatcacgggt ttaacctagc gagggaacac tgaaaaatag aacatttcgg cgaacttagc
      361 gggcactcta attcccgaaa catggggtag gaaaacgaca aaccacacgc ccgctgcgca
      421 aaccacggca gcggcggcct tacgtcctct cgggagcgtc caaagcgcaa acgaaagaag
      481 aatgcacaaa aacgggacgg ccggcaggta gtgataaaac aacatgattc gcggagaaaa
      541 catccaggga atccaaagcg cgccgtaaca agctagaaaa aacattggaa tcggttgagc
      601 aagcggcacc cgccgggcga acacactttt ttttgaccgc cgccggtggc gccaggcctg
      661 caccaccaaa taaatcaccg caaacaatcc tccgagcaac accacagagt ttccggcatt
      721 gtagatgttc ccgattttac cagactccga gtagtcgaca tgcatccaca cgggtttaac
      781 attcaagagc cactgccacg gtttcgattg ataggagtgg gttgccgtaa gattattgtg
      841 ataccaccac atttgacgct gtagttcttt gaactgatgc cacttaaaac ccaataaaaa
      901 gaaatgaatg tacgcagcaa aataagcggc caccgcgccg gtcgcgaata aaaacgcccg
      961 agttaaatag tgaaaaaatt tttcagtgcg acgacgacgc caccactgaa ttacttggtc
     1021 gactccgata atgccgatca cataaaaacc agtccatttg gtactcaatg caaggcccgc
     1081 gcaaaaccca acgccgagag ctagcgaacc cgcaatcccc gactttgggc gcaaacctaa
     1141 atagaggtaa aacgtgagca acataaaaaa tacgaaataa ctgtcgttca tcgcgatccg
     1201 cgattgcgca aagtggagag tctcaagcga tagaaacgcc attgctagca ccgcgatcgg
     1261 gatcgacttt aaaaatcggt acgcgatcag tccggtaaac aaaactgaaa gtgtcccgaa
     1321 caacaccgat ccaaaccgca tccaaaaaaa acggtcgcca aagactttga gaaaccccgc
     1381 cattaccact tttgccaggg gcggatgagt ccattcgtaa gcgaaccctt tagggggact
     1441 agcccacggg tcgtaggctt tggggtcgtt ctgcacataa acttgggcgg tgaatccgtg
     1501 ataaacttcg tcgaaatagt ggtttgcagg gagatgcagc cgcaccaact tggtggtgaa
     1561 tccgaagact agaattccca ctgcaaaata aagccatcgc ttctcggcaa ggagcgcgcg
     1621 aacgaacgat gggttctctg cttccatatt taagaggata gcataaactt ttaggtgaac
     1681 cagagggtcc acgtcgaaat gaacatcaca agatggttaa ccaaatttga tctcctcttg
     1741 ctagcgacct ggggagtata ttttattttt ttattttcgc gcatggtaac gctctcgcca
     1801 gatcaggggc ttcaggtagg acacgaatac ctttggagcg actgggcgag cttgcacacc
     1861 ggaatggttc gcatatttgc gaccaagccc ctgtccgaat ggttcgctta tcacccaatt
     1921 tacgccgacg gtaaattcac ttatccgttt ctggtaaacg caatctccgc tctgttgatt
     1981 cgaatcgggc tgagttttga atcagccatg atggcgccct actatgttgc gtttccttta
     2041 ttgctcctgg ggctaaggcg tcttttcact ttggtcctac aaagccagcg cctcgccgtg
     2101 ctcgctattt ccctattttt tctctcggct gggttaagtt tttacaaagt cctcgcagtg
     2161 tactttgcta ccggaagttg ggacctggct tttccaatat cgagtctcca tagcgcggtt
     2221 ccttcgcaca gttggggggc caacaacttc ttaattgcga tgttgctccc tcaacgcgcg
     2281 tttgccttag gcctacccgt cgcggtctgg gccatttatg gttttttttc ggagcttctc
     2341 cgcgagcgtc ctgcacggta ttcagcggtc aagatggcgg cgagcggcgt ggcgcttggg
     2401 atactgccta tcgtgcacgc ccacagtttg attgcagcgt cgatcgtttt gttcccggcc
     2461 ggagtcatgg cgttaactca gaagcacttg cgaatccgag ctctctactt cgccatgccg
     2521 gctgcggcgg tgggtgcgtt gttttatttt atttggatcg ccggtggaat tcagactcgc
     2581 ttcacgcaac tgttgatcgg tttcaccgcc acctccgtta gcgactggct ctggttctgg
     2641 ggcttcgtct ggggtgtttc gcttccactc gctctctacg gcactttcaa ggccacacaa
     2701 aaagcggcct cgatcgaact ctacctggcg gttgcaggcc tcacgctatt tattttgcca
     2761 aacttctttt tgtttcagcc tacgaagtgg gacaactcta agctctttct ttgggcattt
     2821 ctccttctca cgccagccat ggctcgtgcg ctgggcagcc tgtacgcaag aaaccgctat
     2881 cttggcgttg gagtcgctct cattgcaact cttaccggtg ccctcgagct cgccgacctt
     2941 caatgcgtgt cgcgccatca gtttcagata cttcaacccc aggaactagc actggccgaa
     3001 caggtgcgac tcggaactca ttccatgtcc cgtttcgctg cagcgacgac ccacaatcac
     3061 ttcgtatcga cctggagcgc tcgcgcggtg ttggttgggt acctaggctg gatcggcaac
     3121 tatggtttca accaccaaca aacctacgaa gaactacgcg aaatctatcg agggtcgccc
     3181 cgcacgcttg aacaaatcaa aagccaccga ataaattaca ttgtcttcgg acccgccgag
     3241 cgccgtgag
//
LOCUS       DAOMXO010000215         3249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_16474_length_3249_cov_4.049781, whole genome shotgun sequence.
ACCESSION   DAOMXO010000215 DAOMXO010000000
VERSION     DAOMXO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16474_length_3249_cov_4.049781"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLH57_05515"
     CDS             complement(<1..573)
                     /locus_tag="PLH57_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02104.1"
                     /translation="MQSTMIVATSKIRIFWLALSLGVFTIPNFAGAARQGSAVPPIDF
                     NAIIGSLDKDQCSLVLRDFFGRIEDTEILQDQAVETFFLELLKASERGSIQPRMVIKL
                     VTSRLEKQEPSTKALSIIFRMLSLFSKQRLLSDLELLVFFDRFAHHEVPRVAAAGRGL
                     NLALTFLDELLEKSESPHMIEVVFERYHAIA"
     gene            complement(621..>3249)
                     /locus_tag="PLH57_05520"
     CDS             complement(621..>3249)
                     /locus_tag="PLH57_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK02105.1"
                     /translation="IPEDILEHRPTFQKEFVAELLAPSNSTRSELPTEFVPVYVRIDD
                     IGAFYFPDIDMLLSSKLGTLIENHNSPAHEPLKALHLVIELVPHSSRYIISDEHIDNL
                     TNQLHEWKNALKTRHLSLDLRVSIVTDTNTLPIRHTRLEDISQRNRATPAPGYMGADV
                     VLEQGGPPLPKVVSERLRMNLVAVNPWLLLDRHSQVQYLTERVNKALDLKIDQSTIFS
                     VLSRAKQLLARSAQDIESGPTIEEALIEAVRYGLRNSGGLENRELFLTETVRILAASH
                     GGFSEPIFRIEDLRRIESLEKRLDADSTTASQNAFIGRKNQILEIKQNLLAAARGGFK
                     DKPTNLILLFGESGTGKTELAEQVALEIGAERRYVDMRNYDPNTKFSVTKVAASLHAS
                     NNPQKILTLDELDKSDHGQIRGLLGAVFGNQDGRDEQSSNLKGTTVILILNVDVRNSS
                     YQSLLSAYRAGKPIRPYFVDVIRNALIHGNEASAQKEFREVDSLVKRMSTSAIFLPPI
                     SSDTEQQQKVITKTIRDYEAEKGIRLVIPQATQSFMIERAVENSDGNFRTVERTIPLF
                     IRAAEAHYVLKNPEDQKLKAATHFVLSTVTTENESGLSLSPLQLDDRAGVTAMVRDYS
                     ERFYDRLARHFREKEELLLNARPFAATKADSDLLEFIAASYRGMSEEIKNLRASISGE
                     ILVPSKRKINTASNAAYSHLVDEGRGNQLFSIFPKILSGAIGVDSTARGSSDLEQFAG
                     EIDKGVLTLRMFLERDPTQSLSHANDSLASSFESYLISENIGHSLPYSEKLARAYVRA
                     LGRTLVTEKERIISELVDQGTALRRARNETLPQLHTPTNAPELARCLDLLGTRRQHSE
                     EIERQFNELSERPTSSKKR"
BASE COUNT          802 a          715 c          859 g          873 t
ORIGIN      
        1 agcgattgcg tgatatcgct cgaagaccac ttctatcata tgaggtgatt cggacttttc
       61 gagcagttca tcgagaaacg tgagagcaag attcaagccc ctacccgcag cggccactcg
      121 cggaacttcg tggtgggcaa aacgatcaaa aaaaactaat aattcgaggt ctgaaagcag
      181 gcgttgttta gaaaaaaggc taagcattcg aaaaataatc gaaagcgctt ttgttgaagg
      241 ctcttgtttt tcgaggcggc tagtgaccaa ttttatgacc atccgaggct gaatcgatcc
      301 tctttcggaa gcctttagta gttcgagaaa aaacgtctcg accgcttgat cctgtaaaat
      361 ttcggtgtct tcgattcggc caaaaaaatc gcgaagaaca agcgaacact ggtctttgtc
      421 caaactgccg ataatggcat tgaaatcaat ggggggtacc gcgcttcctt ggcgggcggc
      481 gccggcaaag ttcggaattg tgaaaacacc caaactcaag gccaaccaga aaatgcggat
      541 ttttgaagta gctacgatca tggtgctttg cattacatta atcgaccaaa ttaatcaata
      601 aggtgcgtgc cataatccgt tcagcgtttt tttgaggagg tggggcgctc ggatagctcg
      661 ttgaactggc gttcaatttc ttcggagtgt tgccttcgag taccgagcag gtcgaggcat
      721 cgagctagtt ccggtgcgtt ggtcggtgta tggagctgcg ggagcgtctc atttcgcgct
      781 cggcgtaggg ccgtgccctg gtcgactaat tcgctaataa tccgctcctt ttccgtgacc
      841 aaagtgcgac caagtgctcg cacgtaggct ctcgctaact tttcggaata cggtaacgaa
      901 tgaccgatat tttcactgat taaataactt tcgaacgacg aggcgaggct atcattcgca
      961 tgagagagcg actgcgtggg gtcccgctcc aaaaacatgc gaagagtaag tacgcctttg
     1021 tcgatttcgc cagcaaattg ctcgaggtcg gaggaacctc gagccgtgga atcgactcca
     1081 attgcgccgg agagaatttt tggaaatatg ctgaaaagtt gattgccgcg gccttcgtcc
     1141 acgaggtgag agtaagcagc attgctggcg gtatttattt ttcgcttgct gggtaccagt
     1201 atttcaccac tgattgaggc gcgcaggttc ttaatttctt cggacattcc ccgataggaa
     1261 gcggcaatga attctaagag gtctgaatcc gctttggtgg cagcaaaggg ccttgcattt
     1321 aatagcagtt cttctttttc tcggaagtgc cgcgcgaggc gatcgtagaa tcgttcacta
     1381 tagtcacgaa ccattgcagt tacacctgcg cggtcgtcga gctgtagtgg actaagggaa
     1441 agacccgatt cgttctcggt agtcacggtg ctcaagacga agtgcgtggc ggctttcagc
     1501 ttttggtctt cggggttctt taaaacgtag tgggcctctg cggctcgtat gaaaagtggg
     1561 atagttcgct cgacagttcg aaagttgcca tcgctgtttt cgacagcacg ctctatcatg
     1621 aacgattgag ttgcttgggg aattaccaaa cgaatgccct tttcggcttc gtagtctcga
     1681 attgttttag tgatcacttt ttgctgttgt tcggtgtcag acgaaatcgg cggaagaaaa
     1741 attgccgaag tagacatgcg ttttaccagg ctatcgacct cccgaaattc cttctgagcc
     1801 gaggcttcgt tgccgtgaat caatgcattg cgaattacat ctacgaaata cggacgaatc
     1861 ggcttacctg cgcggtaagc tgaaagtagg gattgatagc ttgagttccg aacgtcgacg
     1921 tttaatatga gaattacggt cgtacccttg aggttggaag actgttcgtc gcggccgtct
     1981 tggtttccga aaaccgcgcc gagcagacca cgaatttggc cgtggtccga tttatcgagc
     2041 tcgtcgagcg tcaaaatttt ctgaggatta ttgctggcat ggagggaggc cgcaactttc
     2101 gtgaccgaaa acttggtatt agggtcgtag tttcgcatgt caacatagcg gcgttccgcg
     2161 ccaatttcta gcgccacctg ttcggcgagt tcagttttcc ctgtgccgga ttcaccaaac
     2221 agcaggatta gattcgtggg tttgtcttta aaaccacctc tggcggcggc taataagttc
     2281 tgtttaattt ctaaaatctg atttttgcgt ccaataaaag cattttgcga agcggtagta
     2341 gagtcggcgt ccagacgttt ttcgagagat tcaatgcgtc tgagatcttc aatacggaaa
     2401 atcggttcgc tgaaaccgcc gtgagacgct gctaaaatac gaacagtttc ggttaaaaaa
     2461 agctcgcgat tttccaaacc accgctgttg cggagtccat agcgtacagc ttcgattagc
     2521 gcttcctcga tagtgggacc gctttcgatg tcttgcgccg aacgtgcaag cagttgctta
     2581 gcgcgactta aaactgaaaa aattgtcgac tgatcgattt ttagatcgag cgctttatta
     2641 actcgttccg ttaagtactg cacctgagaa tgtcgatcga gaagaagcca gggattcacg
     2701 gcaactaaat tcattcgaag ccgctcggag acgaccttgg gcaaaggcgg tcccccttgt
     2761 tcgagcacaa catcggcgcc catatagcct ggcgccggtg tggcgcgatt gcgctgcgag
     2821 atatcttcta aacgagtgtg tcgaatgggt agcgtgttgg tatccgttac aattgaaacg
     2881 cgaagatcta gcgatagatg ccttgttttg agagcatttt tccactcgtg taactgattg
     2941 gtcaaattgt cgatatgttc gtcggagata atatagcgac ttgagtgggg aaccagttca
     3001 atgacaaggt gaagagcttt tagaggctcg tgtgcaggac tattatgatt ttcaattaaa
     3061 gttccgagtt tcgacgagag cagcatgtct atgtcaggaa aataaaaggc tccaatatcg
     3121 tcaatgcgaa cgtagacggg tacaaattct gtcggtaatt cggatctagt tgaattagag
     3181 ggggctagca attcagcgac gaactctttt tgaaatgttg gtcgatgttc cagaatgtct
     3241 tctggaatt
//
LOCUS       DAOMXO010000216         3242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_16517_length_3242_cov_5.818638, whole genome shotgun sequence.
ACCESSION   DAOMXO010000216 DAOMXO010000000
VERSION     DAOMXO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16517_length_3242_cov_5.818638"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLH57_05525"
     CDS             <1..425
                     /locus_tag="PLH57_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02106.1"
                     /translation="NNGWSEVVDLSHFIGHGQTPSCTYPHFCLFGGSLAVPESKANVY
                     GADRNDGLCTYWEGEKRGCLVSHSLLTSYTAECNPGEQKVYIDRIMSTNPCGLHTAPS
                     PTPPPGWCNVDDPFQYMLDTEEWSVELEAITENTRSEF"
     gene            608..1468
                     /locus_tag="PLH57_05530"
     CDS             608..1468
                     /locus_tag="PLH57_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02107.1"
                     /translation="MKFLKVILLASCLTITACGGKDAEDVALTNPSGGNASPVVPNPT
                     NSPTTTPSPTAAPAVCDKEVIQADLFRQVLNGTANNRVYTSASSFYADSYIKAKIRSL
                     GQSSSADDGAGGNNSFTKFRGTVELWRRNSGSTSYSKVASIVVPTTVDADGRPDGISV
                     NTLSGEITWGSQYLRAGASYKVAISNPYTNMKCSNYCTGNSNAYPKVYYNEFYWPQHA
                     SLCNANIGYQWYYISGYGYTCYGARLDMVASCRSEQCSVGPLTSTDTNHTWSVEVFVE
                     GDRTECLPTN"
     gene            complement(1493..2575)
                     /locus_tag="PLH57_05535"
     CDS             complement(1493..2575)
                     /locus_tag="PLH57_05535"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRK02108.1"
                     /translation="MNKLFVLSIVLLTTTGCSSASYLVQAGRGQLAIYNRERPLEEVI
                     QDDRVSLSLRNQLREVPEIKAYVAKTLGVAATSNYKTFSDLKRKYAIWVLSSSARFEL
                     KLKTWSFPIVGTFPYLGFFDEEMAREWSAREQAEGQDTYVRGASAYSMLGFLREPLLS
                     TMLQGRKEDLVNLIFHETLHSHVYLDGQGAFNEQIASYFGDYGELQYLVENFGSESEQ
                     VQIWKKNREDRRRMGALLREFERELAALYENIKNLSNAEKEQKKKLAFDLFKRRVNAV
                     KWHRARTGEYLSERVTNNATVLAFLTYEDEQGLYDLLHEKSKGDLAVSLRLVQEFAKH
                     YASSKAAEKPQRLLESFLRERTKQPL"
     gene            complement(2576..3187)
                     /locus_tag="PLH57_05540"
     CDS             complement(2576..3187)
                     /locus_tag="PLH57_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRK02109.1"
                     /translation="MQGCTDKNPLGRGRNKLAITWFFATVLVGLFSDPFLTDAFARNG
                     QFSFMASYSKSNYGNNSYSTQRRYTFAGAMNLTPVTEIELSFTSSKAFINYDPYQTTT
                     TNDAILGLSLIQVIVPPTWPIQPYVKVGAAQYNRKQTGTINGVPTAPTSTKSPSAIFG
                     GGIRAFFARSFSLKLEITTYLPDMEVDQAKNNYAAQAGLGWHF"
BASE COUNT          835 a          813 c          748 g          846 t
ORIGIN      
        1 aaaataatgg ctggtcagag gtcgttgatc tttcgcattt cattggacac ggccaaactc
       61 cttcgtgcac ttacccgcat ttttgtcttt ttggcggctc actcgcggtc ccagaatcta
      121 aagcgaatgt ttacggcgcc gatcgcaatg acggactttg cacctattgg gaaggtgaaa
      181 agcgtggctg ccttgtgagc cattcactgc ttacgtcgta caccgcggag tgcaatcctg
      241 gtgaacagaa agtctatatc gaccgcatca tgtcaaccaa cccttgcggc ctgcatactg
      301 cgccttcgcc tacgccgccg cccggctggt gcaacgtcga tgatccgttt cagtacatgc
      361 tcgacactga agagtggagt gtggaactcg aagcgatcac cgaaaatacg cgttcggaat
      421 tttaagattc tagccgtgtg tctaatgatt agacgccgat tacaacgaag agcccgcgca
      481 aagaaggatt cgaaaaatgg caccgtgatt gcactaacta gagtttgaga aagagatatt
      541 cgggtcgaat ttacttttaa gtagtcgctc cagttgcgac ccgatgttgt gaagagagga
      601 agtagttatg aagttcctaa aagttatttt gttggcatcg tgcttaacga tcaccgcttg
      661 cggtggcaaa gacgctgaag acgtcgcgct aaccaacccg tcaggcggca atgctagccc
      721 ggtggtacca aacccaacaa actcaccaac cacgactcca tcgccaaccg cggctcctgc
      781 agtttgtgat aaagaagtga ttcaagcaga tttatttagg caggttctga atggaaccgc
      841 caacaaccga gtttacacct cggctagttc gttctacgcc gacagctaca ttaaggctaa
      901 aattcgctct cttggccaat cttcgagcgc ggatgacgga gcaggtggca acaactcgtt
      961 caccaagttt cgtggcacag tagaactatg gcgtcgcaat tccggttcta cttcgtacag
     1021 caaagtagcg agcatcgtag tacctactac ggtcgatgca gacggtcgcc cggatggaat
     1081 ctcggttaac acgctttctg gcgaaatcac ctggggctca cagtatttgc gcgccggtgc
     1141 tagctacaaa gtggcgatct ctaaccctta caccaacatg aagtgtagca actattgcac
     1201 tggcaacagt aacgcttacc ctaaagttta ttacaacgaa ttttattggc cgcagcacgc
     1261 tagcctttgt aacgcaaata tcggctacca atggtattac atctcgggtt acggctacac
     1321 ttgttacggc gcacgcctcg acatggtggc ctcatgccgg tccgaacagt gcagcgttgg
     1381 tccgttgaca agtacagata caaaccacac ttggtcggta gaagtattcg ttgaaggcga
     1441 tcgcacggag tgtttgccta cgaactaatt ggaccgcatt aagattgatt tgctaaagag
     1501 gttgtttggt tcgttcacgt aggaacgact cgagcaacct ctgaggcttt tcggcagcct
     1561 tagaactagc gtaatgtttt gcgaattctt gaacaagtcg taatgacact gcgagatcac
     1621 ctttactttt ttcgtgcaat aagtcgtata gtccttgttc atcctcgtag gtcagaaatg
     1681 ccagtaccgt agcgttgttg gtaacacgtt cggagaggta ctcccccgtg cgggctcgat
     1741 gccacttgac ggcattgacc cgtcgcttaa ataaatcgaa cgctaacttt tttttctgtt
     1801 ctttctcggc gtttgataaa ttcttaatgt tttcgtaaag cgctgctagt tctcgttcaa
     1861 actctcgaag tagcgccccc atcctgcgtc gatcttcacg attttttttc caaatctgca
     1921 cctgttcgct ctcggaacca aaattctcga ctaaatattg aagttcacca tagtcgccaa
     1981 aataactcgc aatttgctcg ttaaaagcac cttggccgtc taaataaacg tgactatgta
     2041 gggtttcatg gaaaatgaga ttcaccagat cctcttttcg accctgaagc attgtagaca
     2101 ataacggctc acgtaaaaaa cccaacatcg agtaagcact agctccccta acataggtat
     2161 cttgcccctc ggcctgctcc cgcgcactcc attcacgcgc catttcttcg tcgaaaaatc
     2221 cgaggtaagg aaaggtcccc acaatcggaa aggaccaagt ttttaatttc agttcgaaac
     2281 gggccgagct acttagcacc caaatggcgt atttacgttt cagatcagaa aacgttttgt
     2341 agttactggt cgcggctact cccaaagtct tggcaacata ggccttgatc tccggaactt
     2401 ctcgtaattg attgcgtaag ctaagcgaca cacgatcgtc ttgtatgact tcttccagcg
     2461 gacgttcccg attatatatg gccagttgcc cgcgcccggc ctgcaccaga taggacgcac
     2521 tagaacagcc cgtcgttgtc aacaacacta tcgagagcac aaatagttta ttcaatcaga
     2581 aatgccatcc aaggccggcc tgagcggcgt aattgttttt agcctgatca acttccatat
     2641 ctggaagata ggtcgtgatc tcgagtttaa gcgaaaatga tcttgcgaaa aaggccctta
     2701 ttccaccgcc gaaaatcgcg gacggactct tggtcgaggt tggcgcggtt ggaacgccat
     2761 tgattgttcc tgtttgtttt cgattgtact gagcagcacc caccttcacg tagggctgaa
     2821 tcggccaggt aggcggtaca atcacctgaa tgagcgacag accaagtatc gcgtcattgg
     2881 tcgttgtggt ttgataagga tcgtaattta taaatgcctt cgaagacgta aacgataact
     2941 caatctctgt aacaggggtt aggttcatag ctccggcgaa ggtatagcgg cgttgagttg
     3001 aataggaatt atttccgtaa ttactttttg agtaggaagc catgaacgaa aattggccgt
     3061 ttctcgcaaa cgcatcagtc agaaaagggt cgctaaacaa ccccaccaac acggtagcga
     3121 aaaaccatgt aatcgctaat ttatttcgcc ctcttccgag agggttcttg tccgtacatc
     3181 cttgcacgtt acactctcca tcaaatactt aatacctagg aatttcggtc ttcaaactct
     3241 cc
//
LOCUS       DAOMXO010000217         3209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_16777_length_3209_cov_4.789157, whole genome shotgun sequence.
ACCESSION   DAOMXO010000217 DAOMXO010000000
VERSION     DAOMXO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16777_length_3209_cov_4.789157"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLH57_05545"
     CDS             complement(<1..798)
                     /locus_tag="PLH57_05545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK02110.1"
                     /translation="MGLLNSNITARVRAQYERYPYPHVPWWKLLFLPGTKQLERLAVP
                     NARTILVAGCGTLQATLVARANPQSQIVALDLSETALRKAQRIAKFCGVGQAIRFEQG
                     DLHELTYPDASFDYLVCTGVLHHCEKPQSALSELARVLADQGTLRLMTYAPKSRSQIY
                     QLQRLVKGLNLSEAKRTLDSLSRTHQLRYAYRSYSDARTKAGFVDGFLHAHDQPIAYS
                     DFEKWCSSLGLEVLRVDLPPGESLTELARALGIDQQTFSNYSTWKQLE"
     gene            complement(786..1319)
                     /locus_tag="PLH57_05550"
     CDS             complement(786..1319)
                     /locus_tag="PLH57_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02111.1"
                     /translation="MTIARHNIFVLICFTVLSVLIATPAQAAPKGQSFAGAGVGFEVL
                     TNGGSGTGFYTTALGGYQFNDTNFGLGLHVGYSNVGGVGIKVLDFGAFAQYLDDVSGM
                     YGRLYVDGIHGSTADGKRRNGVDGRETTAAVGTGLGLLIPSAGSFHLVPEVTYRLAFF
                     KEQVHLIAGTFGVQWDF"
     gene            1370..2122
                     /locus_tag="PLH57_05555"
     CDS             1370..2122
                     /locus_tag="PLH57_05555"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRK02112.1"
                     /translation="MHPILFRIGTFELFSYGLAMAIGVLVSLVVLDSEAKRAGWNRDQ
                     TTRMVVFTFLMGLVGARIVYVFTRINDPGVDILAVLFNARAGFVYYGGLIIGTLYLAA
                     YLAWNKLAVWPRIDGMTMGLCMGLGIGRLGCLLGGCCFGTPTDLPWGVVMANEPSLGH
                     LHPTQLYEFALLMVTFGVLWWRRTRKAYEGELAVWFAGSYAIGRYVLEFWRGDSIRGH
                     IVDNYLSTSQAISIPVLVVAIYFHFKLRKRAS"
     gene            complement(2092..>3209)
                     /locus_tag="PLH57_05560"
     CDS             complement(2092..>3209)
                     /locus_tag="PLH57_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02113.1"
                     /translation="KERALFLKTIQETVDYVMREMADPETGAFYSAQDADSEGREGAF
                     FVWSKNNFVDILVKNGFSETDSLALANHFGVTEAGNFEHTNETVLWTSPQEALDRHDL
                     VAKARSVLFAERERREKPFRDEKSLTGWNALMISGLVWASLALKRHGDPARANEAHQA
                     AQRAFDFLRTTLSQSSNHLWATAKDGKAKIEGFLDDYAFATTAAIDMARLGHLDAIDL
                     AAQWNETIWKEFLDDTGTGFYFTSSRHERLIARPKTIYDQAIPSGTATTLWNSLLLAE
                     LRADSALAAKAEDVLFRLFTPLLNSAHGMAELANASLLWLCSPGVVNFELKELHPFWL
                     VKGNESVDQKKTVCQRQSCVAAPANFSLQEALLRSLK"
BASE COUNT          729 a          796 c          859 g          825 t
ORIGIN      
        1 ttctagttgc ttccaggtcg agtagttcga gaacgtttgc tgatcaatgc cgagggccct
       61 agcaagttcg gttaacgatt cgccgggcgg gagatcgact cgaagcactt ctaaaccgag
      121 cgagctacac catttttcaa aatcagagta agctattggt tgatcgtgcg cgtggagaaa
      181 accgtctacg aaaccagcct tcgtgcgtgc gtcggagtag ctccgatatg catagcgaag
      241 ttgatgggtt cgagaaagtg agtcgagtgt gcgtttggct tcggataagt tcaaaccttt
      301 gaccagtcgt tgaagctggt aaatttgact acgcgatttg ggagcatagg tcatgagacg
      361 aagagtgcct tggtcggcca acacgcgagc aagttcggaa agtgcacttt ggggtttttc
      421 acaatggtgc agcacaccgg tgcataccaa gtaatcaaaa ctggcgtccg gataagtcag
      481 ctcgtgcaga tccccttgct caaatcgaat tgcctgaccg acaccgcaga attttgcaat
      541 tcgctgcgct ttgcgaagag cggtttcact taaatctagg gcgacaattt gtgattgtgg
      601 gttggcgcgc gccactaagg ttgcttgcaa agtgccgcag cccgctacga gtatagttcg
      661 cgcgttgggt acggccaggc gctctagttg tttggtgcct ggtagaaaca acaacttcca
      721 ccacgggacg tgggggtagg gataccgctc gtactgcgcg cgaacgcgcg cggttatatt
      781 agagtttaaa agtcccattg cacgccaaac gtgccagcga tcaaatgaac ctgttctttg
      841 aaaaaagcca atcgataggt gacttcggga acaagatgaa aacttcccgc cgaaggaata
      901 agaaggccaa gtcctgtgcc gacagctgcg gtagtttccc gaccgtcgac gccgtttcgg
      961 cgcttgccgt ccgcggtaga accgtgaatg ccatcgacgt atagtcgacc atacattcca
     1021 ctcacgtcgt ctaagtattg agcaaaggcc ccgaaatcga gaactttaat tccgactccg
     1081 ccgacattgg agtacccgac gtgcaaaccc agtccgaagt ttgtgtcgtt gaattggtag
     1141 ccacctaggg cggtcgtgta gaaaccagtg cccgagccgc cgttggtgag tacttcaaaa
     1201 cccacgcccg ctccggcaaa gctttggcct ttgggagcgg cctgcgccgg agtggcaatt
     1261 aaaacggaca atacggtgaa acaaattaaa acgaagatat tgtgtcgcgc gatagtcatg
     1321 tttaaaaccc tcgttgtcac tttcgccgat tgtttgctac gatgcaagca tgcaccccat
     1381 actttttaga atcggtacct tcgagttgtt ttcctacggc cttgctatgg cgatcggcgt
     1441 acttgtttcg ttggttgttt tggactccga ggccaaacgc gccggatgga accgcgatca
     1501 aaccacgcgc atggtggtct ttacttttct aatgggtttg gtcggcgccc gcattgtgta
     1561 cgtatttacc cgcattaatg atccgggcgt agatattttg gcggtgctct ttaacgcgcg
     1621 tgcaggattt gtttattacg gtggtcttat catcggcaca ctttatctcg cagcttacct
     1681 tgcttggaac aaactcgcag tgtggccgcg tattgatggc atgaccatgg ggctttgcat
     1741 gggactcgga atcggacgcc tcggctgctt gcttggcggc tgctgtttcg gtacgccgac
     1801 cgatttaccg tggggagtgg tgatggccaa cgaacccagt cttggccact tgcacccgac
     1861 gcaactttac gaatttgcat tgttgatggt tacgtttggt gttctttggt ggcgccgtac
     1921 ccgcaaagcc tacgaaggcg agttagccgt ctggtttgca gggtcatacg ccatcggtcg
     1981 ctacgtgcta gaattttggc gaggcgactc aatccgcggc catatcgttg acaattactt
     2041 gtcgacttcc caggccatta gtattccagt gttagtggtc gcaatttatt ttcattttaa
     2101 acttcgtaaa agagcctctt gaagtgagaa attagcgggc gccgccacgc aactttggcg
     2161 ttggcagacg gtctttttct gatcgacgct ctcgttacct tttaccagcc aaaaagggtg
     2221 caactctttt agttcaaagt taacgacccc cggtgaacag agccacaaca aggatgcatt
     2281 ggcgagttcc gccatcccgt gagcgctatt tagtaaaggc gtaaacaaac gaaaaagcac
     2341 atcttccgct tttgcggcta atgccgaatc cgcccgaagt tctgccagca gcaacgagtt
     2401 ccacaaggtg gtagcggtgc cagatggaat cgcttgatcg taaattgttt tcggccgagc
     2461 aatcaatcgt tcgtgcctgg aactggtaaa ataaaatccc gttccggtat catctaagaa
     2521 ttctttccaa atagtttcgt tccattgtgc ggccaaatct atggcgtcga ggtgacccaa
     2581 cctcgccatg tcgattgcgg cggtggtggc aaaagcataa tcatcgagga agccttcgat
     2641 ttttgctttg ccatctttcg cggtggccca taaatgattg gaactttgac taagcgtggt
     2701 tcgcagaaaa tcaaatgcgc gttgggcggc ctggtgagcc tcgttggccc gggccgggtc
     2761 gccgtgacgt ttgagcgcaa gtgatgccca gactaaaccc gaaatcatga gggcgttcca
     2821 gccagttaga gatttttcat cgcgaaaggg tttttcgcga cgttcacgtt cagcaaaaag
     2881 caccgagcgc gcctttgcga cgaggtcgtg gcggtccagc gcctcttggg ggcttgtcca
     2941 tagaaccgtt tcgttagtgt gttcgaagtt acccgcctcg gtgactccga agtggttggc
     3001 taaagccagg gaatcagtct cgctgaaacc atttttaact aagatgtcga caaagttatt
     3061 tttactccaa acaaaaaaag cgccttctcg cccttcgcta tctgcgtctt gcgcgctgta
     3121 gaaagcccct gtttcggggt ccgccatttc tcgcataacg taatcgacgg tttcttgaat
     3181 ggtcttcaaa aaaagtgcgc gctcttttt
//
LOCUS       DAOMXO010000218         3192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_16919_length_3192_cov_3.581128, whole genome shotgun sequence.
ACCESSION   DAOMXO010000218 DAOMXO010000000
VERSION     DAOMXO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16919_length_3192_cov_3.581128"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLH57_05565"
     CDS             complement(<1..327)
                     /locus_tag="PLH57_05565"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK02114.1"
                     /translation="MSVNFRLNDKVVVVTGAGGVIGCRASEVLLEAGAKVALLDIDIQ
                     NLETLGKKLSKIKPSNLMIVECDVSSADSVKKSVDTVVQKWDSVDVLFNNAASKSDNI
                     ESFFAPF"
     gene            complement(344..1339)
                     /gene="pseB"
                     /locus_tag="PLH57_05570"
     CDS             complement(344..1339)
                     /gene="pseB"
                     /locus_tag="PLH57_05570"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HRK02115.1"
                     /translation="MESVHELFDDKVVLLTGGTGSFGKCFVRQICKQFKPRKLIIFSR
                     DELKQFEMAQVFPQSKYPFLQYFIGDVRDRERLGRAFSGVDLVVHAAAMKQVPAAEYN
                     PSEAIKTNIMGAMNVVDAAIDNKIGRVIALSTDKASNPINLYGATKLCSDKLFVAANN
                     FEGKNKTRFSVVRYGNVVGSRGSVVPFFRGLRKSGVIPITDERMTRFFITLEKGVEFV
                     LSCLANSTGGEIFVPKIASMKIVDLARLLAPECEMRVIGIRPGEKLHESLISVEEGRK
                     TFEFSDHYVIAPETSAWATQLGQRVQDGFAYTSDKNLSWLTPAQMNAMIDSTEIE"
     gene            1396..2361
                     /locus_tag="PLH57_05575"
     CDS             1396..2361
                     /locus_tag="PLH57_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK02116.1"
                     /translation="MTHSKPSDQSPKLSKKVVLITGSSGLLGATLLMELRDRYGLVGT
                     YLTHPISLSGTTNYMLDLREKAALFELLERVRPEVIIHTAAAVNVDECETNRELAYQL
                     NVSVPDSLSEYCGANRSLFVHLSTDAFFSEKNVLHDEESAVTPLNYYAETKLLAEDII
                     RKKCSNHLIIRTNFFGWNYQIKSSLAEWIYFLLRDGKTVPAVEDVWFTPILTNTLSST
                     LHEMIEHGLRGTYHVASDRSISKYEFAQQICAAFDFDYSLIIPTKVESLKLKAKRSSN
                     MALSNRKLRRDLPGWQCHIEDEIFKFKELEINGYIRRLRTSLVDN"
     gene            2452..>3192
                     /locus_tag="PLH57_05580"
     CDS             2452..>3192
                     /locus_tag="PLH57_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02117.1"
                     /translation="MSMFTLMKADPFRLIYTPMMVFPDLDLEAYVGLSSFNPKGISNS
                     MQSHQVWFSVYDPSGNFVDRYQVGKLAPNTREFFPVSSLTEKYGFKSPYLVVVHRVPE
                     MDLTNINHEMFLMFRSIVQYRDRFKAFGSVVYEAPYTFNATKRANFIVHSSQVLLSTE
                     IETYLLLLNFTTNSETSHPITCTVDLHHANGKKVSSLRKTVPGFTGCALKLSELVPSA
                     SVSETPSQFTLVGYSNEGILIPLFLNVNN"
BASE COUNT          905 a          758 c          628 g          901 t
ORIGIN      
        1 aaatggagca aaaaacgact caatattgtc agattttgaa gctgcgttat taaataaaac
       61 atcaacactg tcccacttct gcacaaccgt atccaccgac ttttttacgg aatctgctga
      121 ggaaacgtcg cattcaacaa tcatcaaatt tgaaggctta atttttgaaa gtttttttcc
      181 aagggtttct aaattttgaa tatcaatatc aagtagagcg actttcgcgc cggcctctaa
      241 taacacctcg gaggcacggc atccaataac tccgcccgct ccagttacta caacaacttt
      301 gtcgttcaac cgaaagttaa cagacattgc ttcggccccg ctctcattca atctcagtag
      361 aatcgatcat tgcattcatt tgagcaggtg ttaaccaact caaattttta tcgctagtgt
      421 aggcaaaacc atcttgaact cgttgcccaa gctgagtcgc ccaagctgat gtttccggcg
      481 caattacata gtggtcagaa aattcaaaag tctttctacc ttcctcgaca ctaatcaagg
      541 attcgtgaag cttttcgccc ggtcgaatgc caatgacccg catttcacat tctggagcta
      601 aaagtcgtgc aagatctaca attttcatag aggcaatttt cgggacgaaa atttcaccgc
      661 cagttgaatt tgccaaacaa gaaagtacga actcaactcc cttttccaga gttatgaaaa
      721 aacgggtcat gcgttcgtca gtgatgggga tcactcccga tttccgaaga cctcgaaaaa
      781 atggaactac agatccacgg ctgcccacca cgttgccgta tcgaacaacc gaaaagcggg
      841 tcttattttt tccttcaaaa ttatttgctg cgacgaacag tttgtcggaa cacagctttg
      901 ttgcgccgta taaatttata gggttactag ctttatccgt ggaaagagcg atcaccctac
      961 caattttgtt atcgatggcg gcatcaacaa cgttcattgc ccccatgata ttggttttga
     1021 tggcctcgga gggattgtac tcggcagctg gaacctgttt cattgctgct gcatgaacca
     1081 caaggtctac tccgctaaac gcccgaccaa ggcgctctcg atctcggaca tcgccaataa
     1141 aatattgtaa aaatgggtac ttactctgcg ggaaaacctg agccatttcg aactgcttta
     1201 gttcgtcgcg gctaaagata ataagtttac ggggcttaaa ctgcttacaa atttgacgaa
     1261 caaaacactt accaaaggat cctgtccccc cagtcaaaag taccactttg tcgtcaaaca
     1321 gttcatggac agattccatt aatttccctc atttttctat aagctttgct ctttttttga
     1381 attaatgtca atggagtgac acactcaaaa cctagcgacc aatcgccaaa gctgtctaag
     1441 aaggtagtcc taattacagg ttctagcggc ctactcggag ccaccctact catggaactt
     1501 cgagatcggt acggacttgt cggcacctat cttacccatc caatttcgtt atcaggaaca
     1561 accaactata tgcttgacct gcgagagaaa gccgcactct ttgaacttct tgaacgagtc
     1621 cgcccggaag tcataattca cactgctgct gctgtaaacg tggacgaatg cgaaaccaat
     1681 cgcgaacttg cctatcaatt gaatgtttcg gttccggatt cactctctga gtattgtggc
     1741 gccaaccgaa gtttgtttgt gcatttgtca actgacgcat ttttttctga gaaaaacgtt
     1801 cttcatgacg aagaatcggc agttacacct cttaattatt atgccgaaac aaaattactc
     1861 gccgaagaca tcattcgaaa gaaatgctcg aatcacctaa ttattcgtac taactttttt
     1921 ggctggaact accaaatcaa gagtagcctc gcagagtgga tatattttct gctgcgcgac
     1981 ggcaagactg ttccagcagt agaagatgtt tggttcacgc ccatcctaac caatacgcta
     2041 tcttccacat tacacgaaat gatcgaacac ggacttcgtg gaacttacca tgttgcgagt
     2101 gatcgctcga tatccaagta cgagtttgcc cagcaaatat gcgcggcatt tgattttgat
     2161 tactcgctca ttattcccac taaggttgag agtctgaagt taaaggccaa aaggtcctcg
     2221 aacatggctc tttcgaaccg gaaattgagg cgagacctac cgggttggca atgccacatt
     2281 gaggacgaga tattcaaatt taaagagctc gaaataaatg gatatattcg tcgacttcgg
     2341 accagtcttg tggacaattg aacatcttac tggccactgt cttttgaaca aaaatcggcc
     2401 tattcccgcc ttaaaaatag gctgcaattt tcttgttaaa ctggcgaata cttgagtatg
     2461 ttcaccctca tgaaagcaga tccatttcga ttgatatata cgccaatgat ggtttttccg
     2521 gacctggatt tagaagcata tgttggtttg agctctttca acccaaaggg aatttcgaac
     2581 tctatgcagt cgcaccaggt ttggttttcg gtttacgacc cttccggaaa ttttgttgat
     2641 aggtaccaag ttggcaagtt ggcgcccaat actagagagt tttttcctgt atcttcactt
     2701 actgaaaaat atggcttcaa gtcgccatac cttgtcgtag tacacagagt tccagaaatg
     2761 gacctaacca atattaacca tgaaatgttt ttgatgttcc ggtcgatagt gcaataccgc
     2821 gatcgattta aagcctttgg tagcgtggtc tacgaggcac cctacacatt taatgccact
     2881 aaaagagcta attttatcgt acatagcagt caggttttgc tcagtacgga aatcgaaaca
     2941 tatctccttc ttttaaactt cactacaaat tctgagacct ctcacccgat tacctgcaca
     3001 gtggaccttc accacgctaa tggaaaaaaa gtttcaagcc tccgaaaaac tgtgcccggc
     3061 tttactgggt gtgcactaaa gctttcagaa cttgttccga gcgcttcagt cagcgaaact
     3121 ccgagccaat ttactttggt tggctattcg aacgagggaa tcctaatccc attattttta
     3181 aacgtaaata at
//
LOCUS       DAOMXO010000219         3187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_16960_length_3187_cov_4.967114, whole genome shotgun sequence.
ACCESSION   DAOMXO010000219 DAOMXO010000000
VERSION     DAOMXO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16960_length_3187_cov_4.967114"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1980
                     /gene="lon"
                     /locus_tag="PLH57_05585"
     CDS             <1..1980
                     /gene="lon"
                     /locus_tag="PLH57_05585"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRK02118.1"
                     /translation="ELLEKVISLGKMLSPDILMVLDDITDPGRLADLVASNLGLKVAE
                     AQLILETTSPIERIKRITDILNKELEVLSLQAKIRSQAKDEMTKTQKEYFLREQIRAI
                     KTELGDADSKGEELQELKEKILACRMPPEVEQEALKQMARLERMHPDASEASMLRTYI
                     DWLIETPWAKATKDNLDIARAKQILDEDHYNLTKIKERILEYLAVRKLKEKMKGPILC
                     FAGPPGVGKTSLGKSIARALGREFVRISLGGVKDEAEIRGHRRTYVGALPGRIIQGLK
                     QAGTNNPVFVLDEIDKLGSDFRGDPSAALLEVLDPEQNNAFRDHYLNVPYDLTHVMFL
                     ATANVLDTIPSALRDRMEIIQLPGYTEEEKVRITQNYLIKKQIGENGISPRHIEFTEE
                     GVKNVIAGYTREAGLRSLERNIGTICRKTAKRVAEGNEEKVIVNKETVAKLLGPATFV
                     REDEQDKDEVGIATGLAWTQFGGEILYIETTTMKGKGAITLTGQMGEVMKESAQAAMG
                     YIRSKASAYGIDEDVFSKLDVHVHIPAGAIPKDGPSAGITMATAIISKLTNVPIRKDV
                     AMTGEITLTGKVLPIGGLKEKALAAMRHGVKTIVIPDKNKKDLEDIPEEYREKLTFVP
                     VKTLDEVLEVTLTKKKKSGKPSKLRKVAGGLDRAA"
     gene            2090..2497
                     /locus_tag="PLH57_05590"
     CDS             2090..2497
                     /locus_tag="PLH57_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK02119.1"
                     /translation="MAKTAMVVDDVAFARQVIKEILTRAKYNVVAEASNGDEAIAMYE
                     KYRPDLVTMDVVMPIRGGIEATRTIIDRNKSARIIMISALAHEQLLMEAINAGARDYI
                     LKPFGAEDLLKAADKVLKDDEDEEPKGGAHAKI"
     gene            2484..2936
                     /locus_tag="PLH57_05595"
     CDS             2484..2936
                     /locus_tag="PLH57_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02120.1"
                     /translation="MQKFKRDHLFPDSIDFYGKGQVKSESPEKFQGSGLPETNPVIVT
                     RLIDSDFLGLPDSATASAGTCLFQFSELPQNRSMALEIANILASQLAHKVSQEIGANV
                     IVRPSQLSTLRYDESALQTQGSRVYSYQSEHETFFIRMLFIAAVAGNA"
     gene            2975..>3187
                     /locus_tag="PLH57_05600"
     CDS             2975..>3187
                     /locus_tag="PLH57_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02121.1"
                     /translation="MVRLLILGFQVAAFAAVVLLAAQIPLKKDIKGRITTVSDYVAEF
                     TSKDFIQAPIRWAGSKFDFIAGHGQKA"
BASE COUNT          956 a          755 c          765 g          711 t
ORIGIN      
        1 gagctacttg aaaaagtaat aagccttgga aaaatgcttt cgcccgatat tctcatggtg
       61 ctcgacgata ttaccgaccc cggtcgctta gccgaccttg tcgctagcaa cttgggttta
      121 aaggtagctg aagcccaact aatactcgaa accacaagtc cgatcgagcg catcaaacga
      181 atcaccgaca tcttaaacaa agaactcgaa gtactttcgc tacaagccaa aattcgttca
      241 caagctaaag acgagatgac gaaaacgcag aaagaatatt ttctgcgtga acagattcgt
      301 gcgatcaaaa ccgaactggg tgacgccgat tcaaagggtg aggaattgca ggaattaaaa
      361 gaaaagatcc tcgcttgtcg catgccgccc gaagttgaac aagaggcgct caaacaaatg
      421 gcgcgattgg aacgcatgca tcccgacgct tcggaagcct caatgcttcg aacgtacatt
      481 gattggttga ttgaaactcc gtgggcaaaa gccaccaaag acaatctaga catcgctcgc
      541 gctaaacaaa ttctcgatga agatcactac aacttgacca agattaaaga acgaattctc
      601 gaatatctag cagtgcgcaa gttgaaagaa aaaatgaagg gccccattct ttgtttcgca
      661 ggtcctccag gcgttggtaa aacaagtctc ggaaaatcaa tcgcacgtgc tcttggccga
      721 gagttcgtaa gaatcagcct tggcggagtt aaagacgaag cggaaattcg gggacaccgc
      781 cgcacatatg tgggggcatt gccgggccgt attatccaag gcttaaaaca ggcgggtaca
      841 aataatccag tattcgtgct cgacgagatc gataaacttg gtagcgactt ccgtggtgac
      901 ccttcggctg ccctactcga agtgcttgat cctgaacaaa ataacgcatt ccgcgaccat
      961 tacttgaacg tgccctacga cctaacacac gtcatgtttc tggccaccgc gaacgtgctc
     1021 gacacaattc cttcggccct aagagatcga atggaaatca ttcaactccc cggctacacc
     1081 gaagaagaaa aagtacgtat tacgcaaaac tatctgatta aaaaacaaat tggcgaaaac
     1141 ggtattagtc cgcgacacat tgagttcaca gaagaaggtg taaaaaatgt gattgctggc
     1201 tacacccgtg aagcaggttt acgtagtctc gagcgaaaca ttggtactat ttgccgaaaa
     1261 actgcgaagc gtgtggccga aggtaacgaa gaaaaggtta tagttaacaa agaaactgta
     1321 gctaaactct taggaccagc aactttcgtg cgagaagacg aacaagacaa agacgaagtg
     1381 ggtatcgcga ctggccttgc ttggactcag tttggtggag aaattcttta tattgaaacc
     1441 accacaatga agggcaaggg tgccattact cttaccgggc aaatgggtga ggtcatgaag
     1501 gaatccgctc aagcagccat gggctacatt cgctcgaagg cctcggcata cggaatagac
     1561 gaagacgtat tctccaaatt ggacgttcac gttcacattc cggcaggcgc catccctaaa
     1621 gatggtcctt cggccggaat aaccatggcc accgccatta ttagcaagtt aacgaacgtg
     1681 cccatacgca aagacgttgc catgaccggt gaaataaccc tcaccggtaa ggtgcttccg
     1741 attggcggac taaaggaaaa ggcgctagcc gccatgagac acggggttaa aaccatcgta
     1801 atccccgata aaaacaaaaa agacctcgaa gacattcccg aggaataccg ggaaaaacta
     1861 accttcgtgc cagtaaaaac tctggatgaa gttttggagg ttacgctcac aaaaaagaag
     1921 aaatcgggaa agccgtcaaa gctgcgtaaa gtagctggcg gcttggaccg cgccgcctga
     1981 attcttctcg accgtagctc tttcaattca acgcattacg tcaatcgata ggcatttgct
     2041 tcccatccct actttcgaga gtcgtgttac tctctagaaa gggggaacgg tggccaaaac
     2101 agcaatggtg gtcgatgatg tcgcgtttgc tcggcaggta atcaaagaaa ttcttacgcg
     2161 cgcaaaatac aacgtagtgg cagaagcctc caacggcgac gaggccatag cgatgtacga
     2221 aaaatatcgg cctgatttgg taaccatgga tgtcgtcatg ccgattcgcg gcggtatcga
     2281 agccacacgt acgatcatcg atcgcaataa gtccgccaga atcataatga tatcggcgct
     2341 ggcccacgaa cagctattaa tggaagccat taatgcggga gctcgcgact acatacttaa
     2401 accgttcggt gctgaggacc ttttaaaagc ggctgataag gtccttaaag acgatgaaga
     2461 tgaagagcct aagggtggtg cccatgcaaa aatttaaacg tgatcatctg tttccggatt
     2521 cgatcgattt ctatggaaaa ggccaagtga agagtgaatc gccggaaaaa tttcaaggca
     2581 gtgggcttcc ggaaacaaat ccggtcatcg tcacacgact catagacagt gactttctgg
     2641 gactgccaga ttctgccacc gccagcgcag gtacatgctt gttccaattc agcgaattac
     2701 cgcaaaaccg ctcgatggca ctcgaaatag ccaatatact ggcgtcacag ctggctcaca
     2761 aagtatcgca agaaatcggg gcaaatgtga tcgttcgccc ttctcaactt tctacacttc
     2821 ggtatgacga atctgccctg caaacacagg gatcaagagt atattcatac caaagtgagc
     2881 acgaaacttt tttcattcga atgctgttta tcgctgcggt cgcaggaaac gcctaacgtt
     2941 tctaggacct taagcgctct gaaaggagaa ctcggtggta cgtttactca ttctcggttt
     3001 tcaagtggct gctttcgctg cggtagtgtt gttagcagct caaattccct taaagaagga
     3061 cattaaaggt cgaatcacca cagttagtga ttatgtcgca gagtttactt ctaaagattt
     3121 tattcaagcg ccgattcggt gggcgggatc caaattcgat tttatcgcag gtcacggtca
     3181 aaaggcg
//
LOCUS       DAOMXO010000220         3149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17257_length_3149_cov_4.344538, whole genome shotgun sequence.
ACCESSION   DAOMXO010000220 DAOMXO010000000
VERSION     DAOMXO010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17257_length_3149_cov_4.344538"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLH57_05605"
     CDS             complement(<1..191)
                     /locus_tag="PLH57_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRK02122.1"
                     /translation="MNSHWHMKSESETFDELSTSVHSGLHPEEVDRRRVSYGSNTLEK
                     AQRKSPWRILLGQFTNVLIL"
     gene            321..1040
                     /locus_tag="PLH57_05610"
     CDS             321..1040
                     /locus_tag="PLH57_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRK02123.1"
                     /translation="MRATIKSYLTDQRFGATLERARHWLYLALGLFLTGLFVKLSHNV
                     LYDAEDRSWIQHWDNVVLLFIAEHRSGPVTASLIDITALGSVTIVSLIGFVFLTVFYL
                     LRDKCGILQLVLASFGAGFWTWAFKSFVERPRPTIVTQLVEVTGHSFPSGHSLAAAAL
                     YFTFATMAGKHFGSWPRALALQGLAALVIVSVAFSRVYLGVHYPSDVISGALFGAAWA
                     YLVAGLVATMQKHYLKQRRSQ"
     gene            1037..1684
                     /locus_tag="PLH57_05615"
     CDS             1037..1684
                     /locus_tag="PLH57_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRK02124.1"
                     /translation="MSDYNFKRHLPLSLRLWHWFNAVVLLGLLGTVLLRKTFLSWRTN
                     SALIEAQFAEAGITVPPELAKKVAVAIRNPLWDWHVYLGFALLALYLARILVAVFVEK
                     ECVYLRVLKSAVQIKSLPPGKKPRALHYTLVKAGYAAFYFVTALMLITGLLLNFKSEF
                     GIAKHLIETIKETHEFMMWFFVVFIGGHLVGVVLAETRGDQGIVSNMINGGPKNS"
     gene            1732..>3149
                     /locus_tag="PLH57_05620"
     CDS             1732..>3149
                     /locus_tag="PLH57_05620"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK02125.1"
                     /translation="MNKKALAVLTLLCLAVYFRILDHEFLIWDDDANIYKNYLLLKPL
                     GTALSFFWTNAYYSLYIPVTYTAWAIIGKAAIYFGGIDSHFTFAPKYFHAVSLALHTI
                     TSYLVFFAIWHVLRRIRSTEKLPTDEANSLRRDWFWAVLAAAVFMVHPVQVETVAWAT
                     GTKDLLSSALAMASLVLYLLFAEKRFRQPSNSKTPWALYAIASICFFLSVLAKPSTVT
                     VPLIVLAVRRGIMRIPIKKDLAWLLPWFAFGGWITIFTSGLQPLALQKDVAAAWARPF
                     VMGDAFAFYYYQIFIPLSYTPVYGRTPSSVLSTGLAFGTALVFFGSAYAAWKARLKRP
                     WCWGAFCIFTVALLPVSGIVTFVFQDISTVADRYLYLPMFGVVFALALALRRTNRPVI
                     KYIALLVVVLLAGRSFFQTAIWKNNLTLFAHADHARPQNFFIKNNYGYSLMEAGRVAE
                     AIPYFQKAIEANPKHERAWTNLG"
BASE COUNT          706 a          802 c          719 g          922 t
ORIGIN      
        1 agaattaaaa cgttggtaaa ctgccctagt aaaattctcc atgggctttt tcgctgcgct
       61 ttctctaagg tgttggaccc ataagaaacc cttcggcgat cgacctcttc cgggtgcaac
      121 ccactgtgaa cgctggtact aagttcgtcg aaggtctcgg actccgactt catatgccaa
      181 tgcgaattca aatgcgtctc cttaaattat tttcgccccg cgccgtaatg gaatttcaac
      241 ctagtgcgtt ccagtcgttt accattttct tttcataaga tagtgtccct ttgctcaagg
      301 agcaatagta ggtgatccac atgcgggcaa ccatcaaaag ctatctcacc gaccaacgtt
      361 ttggtgcaac attagagcgc gcccgacact ggctgtacct cgctctcgga cttttcctaa
      421 cgggactttt cgtcaaacta tctcacaacg ttttatacga cgccgaagac cgcagctgga
      481 ttcaacattg ggacaacgtg gttttgctct ttatcgcaga acatcggtcc ggcccagtaa
      541 ctgcgagtct gatcgatatc acggccctgg gttccgtcac cattgtttcg ctaattgggt
      601 ttgttttttt aacggtattt tatcttcttc gcgacaagtg tggaattctc caactggtat
      661 tggcttcttt tggcgctggg ttttggactt gggcctttaa gagcttcgtc gaacgccccc
      721 gacccacaat agttacccaa ttagtagaag tcacagggca ctcgtttccc agtggtcatt
      781 cgcttgcggc ggcggccttg tattttacat tcgctaccat ggccggaaag cactttggtt
      841 cgtggcctcg tgcgctcgcg ctacaagggt tggccgctct ggttatagtc agcgtagcat
      901 tttcacgcgt ttacctcggc gtccactacc caagcgatgt gataagtggt gcactttttg
      961 gtgccgcctg ggcttacctt gtcgccggtc ttgtagcgac gatgcaaaaa cactatttaa
     1021 aacaacggag atcccaatga gtgattataa tttcaaacga caccttcccc tgagccttcg
     1081 cctctggcat tggttcaatg cggttgttct gcttggtctt ttgggaactg tgcttttacg
     1141 caaaaccttt ctcagttggc gcactaattc cgcactcatc gaagctcagt ttgcggaggc
     1201 tggaataact gtgccccctg aactcgcaaa aaaagtggct gtggccattc gtaatcctct
     1261 gtgggattgg catgtatact taggctttgc gttgctcgca ctctacctag cgcgaatctt
     1321 ggtagccgtt tttgtagaaa aggaatgtgt ttatttgcgc gtcttaaaaa gcgcggtaca
     1381 aattaaatcc ctacccccag gaaaaaaacc gcgcgctctg cattacacac tcgtcaaagc
     1441 cggttacgcg gccttctact ttgttaccgc cctcatgttg atcactggtc tccttttaaa
     1501 cttcaagtct gaattcggaa tcgccaaaca ccttattgaa acgattaaag aaactcatga
     1561 attcatgatg tggttttttg tcgttttcat cggtggccat ctagtgggcg ttgttttagc
     1621 ggaaacacgt ggagaccaag gaattgtctc aaacatgata aatgggggac caaaaaatag
     1681 ctaaatcaca gaatgttcgg tctcgtcccc aagcggagat taaccagtaa catgaataaa
     1741 aaagctctcg ccgttctaac gctactttgt ctggcggtgt actttcgaat tctcgatcac
     1801 gagtttttaa tctgggacga cgacgccaat atttataaaa actatttact actcaaaccg
     1861 ttaggaacgg cgctcagttt tttttggacc aatgcctact attcacttta tatccccgtc
     1921 acctataccg cctgggcaat catcggtaag gccgcaatat atttcggggg catcgactcg
     1981 cactttacct tcgctccaaa atactttcat gcagtgagcc tggcactaca caccatcacg
     2041 tcctacctgg tgtttttcgc catctggcac gtattgcggc gaattcgttc aactgagaaa
     2101 ttgccaaccg acgaagcaaa ctcgctacgc cgtgactggt tttgggcggt actcgctgct
     2161 gccgttttta tggttcaccc ggtgcaagtg gaaaccgtcg cctgggccac cggtactaaa
     2221 gatcttcttt caagcgcgtt ggcgatggcc tcgcttgtac tttatttatt gtttgctgaa
     2281 aaacgatttc gacaaccgag caacagcaaa acaccctggg ctctttatgc cattgctagc
     2341 atttgttttt ttctgagtgt cttggctaaa cccagtacag tcaccgtgcc gttgattgtt
     2401 ttagccgtta ggcgcggaat catgcgaatc ccgatcaaaa aagatcttgc atggctgctt
     2461 ccctggttcg ctttcggagg ctggatcact attttcacta gtggtttgca acctcttgct
     2521 ttgcaaaaag atgtcgccgc agcgtgggcc cgtcctttcg taatgggaga cgcgtttgcc
     2581 ttttactatt atcaaatatt cattccactt tcgtatacgc cagtttacgg gcgaactccg
     2641 agcagcgtat tatccacggg gttagctttt ggtaccgcat tggttttttt tggctccgcc
     2701 tacgccgcct ggaaagctcg cctaaaacgg ccttggtgtt ggggcgcctt ttgcatcttt
     2761 accgtggcac tattgccggt atcaggaatc gtgacgtttg tttttcagga tatttccact
     2821 gtggccgatc gttatttgta tttgccaatg tttggagtgg tattcgcttt agcgcttgct
     2881 cttcgtcgca ctaaccggcc cgtgattaag tatatcgctt tgctggtggt ggtgttgctc
     2941 gcagggcggt ccttctttca aactgcgatt tggaaaaaca accttaccct atttgcccac
     3001 gccgaccacg cgcgccccca aaattttttc attaagaata actatgggta ctcactcatg
     3061 gaagctgggc gagtcgccga ggcgataccc tattttcaaa aagcgattga agcgaatcca
     3121 aaacacgaac gggcctggac caacctagg
//
LOCUS       DAOMXO010000221         3148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17263_length_3148_cov_4.520854, whole genome shotgun sequence.
ACCESSION   DAOMXO010000221 DAOMXO010000000
VERSION     DAOMXO010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17263_length_3148_cov_4.520854"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLH57_05625"
     CDS             complement(<1..626)
                     /locus_tag="PLH57_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRK02126.1"
                     /translation="MFHQFLEWSDNSGLSQWLLSICTFAVVLLVSLIARRVIWRRLEI
                     WARSTQSPLVATFLIELRFPVVVFLIITALGIGMQMAPAAIKAYPVVGYGSKVTFIIF
                     SVWLLERILSAFFRSQSLPTFFAGSTKTLLLTVSRLFLLAFGLLVVLDTVGISITPIL
                     ASLGVGSVAVALALQDTLGNFFSGLYILIDKPIRIGDFVKVDEGIEGHV"
     gene            complement(631..1362)
                     /locus_tag="PLH57_05630"
     CDS             complement(631..1362)
                     /locus_tag="PLH57_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRK02127.1"
                     /translation="MKELYKQLSRLSEVHLILDFDGTLIPIRDNRDDLSLPLPTLRAL
                     KAITNLPVTILSGRDDEDLKTAFQDFSFQLVARNGFEESATLNQQGVQVRLRLSELLP
                     QTEVAATLIEAKGAMTSLHFRHLKNDNLGPVLLELVSRTIKELGLESGWHAYLGKMVV
                     NVEPRAVSKAKFVADYLAKNTHASVLFAGDDSNDFPVFELNHPRLTRVFVYPSDSPSA
                     LQWSDVKKMPREDFLELLEQLGQRS"
     gene            complement(1359..>3148)
                     /locus_tag="PLH57_05635"
     CDS             complement(1359..>3148)
                     /locus_tag="PLH57_05635"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRK02128.1"
                     /translation="IVNDSSAWYRRGKTARFYIFGLFVAIAAIVIFISTLVGKWTISR
                     PMRDLAFILKDAMRFGNPRARLKSLSKTEFAPLVRDLRQIAHEFQRLRGSDTESEAGI
                     WTAERLRSYVSRNLSDLDFSVVANREPYIHDRKPDGQIQVLQPASGLVTGVEPILRAC
                     NGTWIAHGSGTADKDTVDANNSLRVPPENPEYSLRRVWLTAEEEEGYYYGFSNEGLWP
                     LCHIAHQRPRFDEKDWEQYIKVNKRFADAFKEQFGNKRVTVLIQDYHFSLLPKMIREI
                     SPDSLILLFWHIPWPNPEAFGICPWRQEILDGMLAADVLGFHTQFHCNNFIETVDRYV
                     ESRIDREFYSIQTQGHECRIRAFPISISTERDRFRSEPVSIRKNHGLPDDTIVAVGVD
                     RVDYTKGIIERIKGVRRFAERRPDLAKRFTFIQIGAPSRVHIPVYRQLNEDVQKLVDE
                     TNLVFRETLGRDLIILKLHHHNPSDIQPYYEQANICLVTPLHDGMNLVAKEYVAARAT
                     EDGVLILSQFAGASREFREALIVNPYGPDDIASNIIAACEMTPEDQRNRMRRMRKTVK
                     ENNVYRWGHRLLKELNDVRQFRQIGGPSD"
BASE COUNT          850 a          742 c          757 g          799 t
ORIGIN      
        1 acatgtcctt cgattccttc gtcaaccttt acgaaatcgc cgattcgaat cggtttatcg
       61 attaaaatat atagaccgct gaaaaagttt cccaaggtat cttgaagagc tagggccacc
      121 gcgaccgaac ctacaccgag cgaagcaaga attggggtga tggaaattcc gaccgtgtcg
      181 agaacaacta gaagtccaaa cgccaacaag aacaagcgtg aaaccgtaag taaaagagtt
      241 ttggtgctcc ctgcaaaaaa agtcggcaaa ctttgggaac gaaaaaatgc agacaaaatt
      301 ctttcaagca accaaacact aaaaattata aaggtgactt tagagccgta gccgacgacc
      361 ggataagcct tgatcgcggc tggagccatc tgcattccaa tgccgagcgc ggtgattatc
      421 aaaaatacga cgaccggaaa acgaagttcg atcagaaaag tggcgacgag cggcgattgc
      481 gttgatctcg cccatatttc taaacgtctc cagattacgc gtcgggcaat caaagagacc
      541 agtaaaacaa ccgcaaacgt gcaaatcgag agcagccatt ggcttaggcc agaattgtcg
      601 gaccattcaa ggaactgatg gaacatatta ctaactcctt tgtcctaact gttcgaggag
      661 ctctaaaaaa tcttcgcgcg gcattttttt tacatccgac cattgaaggg cggaaggtga
      721 atcgctcgga tagacaaaaa ctctagtgag gcgaggatga tttagttcaa aaaccggaaa
      781 gtcattactg tcgtcgcctg caaacaacac gctcgcgtga gtatttttgg ctagatagtc
      841 agctacaaat ttggcctttg aaacagctct gggctcaacg ttgaccacca ttttacctag
      901 ataggcgtgc catccgctct cgaggccgag ctctttaata gtgcgcgaga ctagttctaa
      961 taaaactggt cctaagttgt cattttttaa atggcgaaaa tgcaaagaag tcatagcgcc
     1021 tttggcttca atcaaggtcg cggcaacttc tgtttgtggc aacaattcgg aaagcctcag
     1081 tcgtacctgc accccctgct ggtttaaggt ggcggattct tcgaagccat tgcgagcgac
     1141 taattggaac gaaaaatctt gaaacgcagt ttttaagtct tcgtcatctc ggcccgatag
     1201 tattgttaca ggcaggtttg tgatcgcctt tagcgcccgc agagtaggca gtgggagcga
     1261 taaatcgtct cgattatcgc gaatcggaat gagggttccg tcaaaatcca aaatgagatg
     1321 cacttcggat agacgcgaaa gctgtttata aagctccttc aatctgaggg ccctccgatt
     1381 tggcgaaatt gtcgtacgtc attgagttcc ttgagcagac ggtggcccca tcgataaacg
     1441 ttgttttctt tcaccgtttt gcgcatgcgc cgcatgcggt tgcgctgatc ctcgggggtc
     1501 atttcacacg cggcaataat gttagacgca atgtcgtcgg gtccatatgg gttgacgatc
     1561 aaagcctctc tgaattcgcg ggaagcgccc gcaaattgac taagaataag tacaccgtct
     1621 tctgttgcgc gggcagcgac atactctttt gcaactagat tcatgccatc atggagcggc
     1681 gtaactaaac agatattagc ttgttcatag tacggttgaa tgtcagatgg attatgatgg
     1741 tggagtttga gtattatcaa atctcttccc agcgtttctc ggaagactag attggtctcg
     1801 tcgaccaact tttgaacatc ttcatttaac tggcggtaaa ctggaatgtg gacacggcta
     1861 ggagcgccaa tctgaataaa ggtaaatctt tttgcaaggt cgggccttcg ttcggcgaat
     1921 cttcgtaccc ccttaattct ctcgataatt cccttggtgt agtccacccg atcaacccca
     1981 acggctacaa tcgtgtcgtc tggcaggccg tggtttttgc gaatagacac gggctcactc
     2041 cggaatcgat cacgttctgt gctaatgcta atcggaaacg cacgaatgcg acactcgtgt
     2101 ccctgggtct gaatgctgta aaactcacga tcaattcgcg attctacgta gcgatcaacg
     2161 gtttcaataa aattgttgca gtgaaactga gtgtgaaacc cgagtacgtc ggcggccagc
     2221 attccatcta gaatttcttg gcgccacgga catatgccaa acgcttcagg gttgggccaa
     2281 ggaatgtgcc agaataaaag aatcaatgag tctggtgaaa tttcgcgaat catctttggg
     2341 agcagcgaaa aatggtagtc ttgaatgagc accgtaacgc gcttgtttcc gaactgctct
     2401 ttaaaagcat cggcgaaacg cttgtttact ttaatgtact gttcccagtc tttttcatcg
     2461 aaacgcggac gttggtgagc aatatggcaa agcggccata gtccttcatt ggaaaatccg
     2521 taataatagc cttcttcttc ttccgcggtc agccaaaccc gtcttagcga gtattcggga
     2581 ttttccgggg gaactcgcag cgagttattt gcatcgaccg tatctttgtc tgctgttccg
     2641 gaaccgtggg cgatccaggt tccgttgcaa gcgcgtaaaa taggttccac tcccgttacg
     2701 agaccactag caggctgcaa gacctgaatc tgaccgtcag gtttcctgtc gtgaatatat
     2761 ggttcccgat tcgcgacgac tgaaaaatct agatcactta aattgcgact cacgtaacta
     2821 cgtagacgtt cggcggtcca aatgccagct tccgactcgg tgtcggaacc tcgcagtcgc
     2881 tgaaactcat gagcgatctg tcttaaatct cgcaccaagg gcgcgaattc agttttcgat
     2941 aagcttttta aacgagccct cggattgccg aaacgcatgg cgtctttgag aatgaacgcg
     3001 agatcgcgca ttgggcgact gattgtccac ttgccaacaa gcgttgaaat aaaaattaca
     3061 attgcagcaa ttgcgacgaa tagcccgaaa atataaaagc gagcggtttt accccttcga
     3121 taccaggccg aagagtcgtt tacgatgt
//
LOCUS       DAOMXO010000222         3146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17276_length_3146_cov_5.326755, whole genome shotgun sequence.
ACCESSION   DAOMXO010000222 DAOMXO010000000
VERSION     DAOMXO010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17276_length_3146_cov_5.326755"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLH57_05640"
     CDS             <1..706
                     /locus_tag="PLH57_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HRK02129.1"
                     /translation="EFVSYFFTKLLGEKSGSVLVGFLGGFVSSTAVLLSSARQSKKNP
                     KNLRNFVQSALAAKIAAIVQLLVIVGMVSPTLLYQLFTPMGLIIAYGATFLFYVSRGK
                     GAKAPTSALSLRSPLDLRGVLRLATLLAAILAAVSFAKLWFGDQGTLVIAFLTGLFEL
                     HGITLATATMFNHQILDLQGAKTAILLGTGASFAAKIAISWGLSTQPFARTLTLAFFP
                     MLAIAFLFGLLPFLMH"
     gene            728..1159
                     /locus_tag="PLH57_05645"
     CDS             728..1159
                     /locus_tag="PLH57_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRK02130.1"
                     /translation="MKNLFAIKKHSLSVDIALTLVRVVMGYAFVLHGWGKIQTPFSWM
                     GPESPVPGVFQALAALSEFGGGIGLILGLLTTLSTFGLTCTMLVAAAMHKFVFGDPFV
                     ATGKGGGSYELAAIYLVLSVLLFVAGPGRFSADRTLFGNKN"
     gene            complement(1164..2099)
                     /locus_tag="PLH57_05650"
     CDS             complement(1164..2099)
                     /locus_tag="PLH57_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HRK02131.1"
                     /translation="MNKLRLKPNKPWTFAVFFFALLVANFTYAAETGLQTIHFQGQLT
                     DSNGTPAHGAREMRFGLYVGGSRVWFARYSAVQVNAGSYSVSLGADQQGAAALDPLNG
                     TALPLASLPIRSALTAGLAPGTSVEIELEIHNGTVFETLTPRVPMSAVFFALSADTLD
                     GLDSENFAQFNGTGALVSQGGVPVVDANGNWIGPSSGLVGPTGPQGPAGANGATGPQG
                     PTGPQGPAGALNYQLRTVAANVTSLVVDCLEGHYALSGGGDCITGGSNRMAASCPAAS
                     ATATTCSNSLPSRYWLVRCASANANNKAFALCVPQ"
     gene            complement(2108..2407)
                     /locus_tag="PLH57_05655"
     CDS             complement(2108..2407)
                     /locus_tag="PLH57_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02132.1"
                     /translation="MRFVNSIRLGLIVVVAAQVSGCRFSDRINGLVDSLKGKVPVPTA
                     TPVSEPSGGSGSTPTTTGTNTQFGGLSGGQAQTLSSPSFRVNGVIVGSRPERQIQ"
     gene            2661..>3146
                     /locus_tag="PLH57_05660"
     CDS             2661..>3146
                     /locus_tag="PLH57_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02133.1"
                     /translation="MTFYKVRRPVFATALMLFTASTSATAKVWSGGQKNNGMERLPKR
                     AQEILRELPGGQITLNFVLERATEASDSFRAASAGVQALDASYHRSRAATDFQYFLRG
                     TRSLDRREATNSFSTTSNEENKFTLGVSRSFETGTDLSGEISQSQTKLGFATIAPIDE
                     TE"
BASE COUNT          724 a          853 c          767 g          802 t
ORIGIN      
        1 tgaattcgtc agttattttt tcactaaact gctcggtgaa aaaagcggtt ctgttctagt
       61 aggctttctt ggtggcttcg tatcgagcac cgctgtgctg ctttcttctg cacgtcagtc
      121 gaaaaaaaat cccaagaatt tgaggaattt tgttcaatcg gccttggctg ccaagatcgc
      181 ggctattgtt caacttttag tgattgtcgg tatggtttcg cccaccctat tgtaccaact
      241 ctttactccg atgggactca tcatcgcata cggcgcaaca tttttgtttt atgtctcacg
      301 cggtaaaggc gccaaagccc cgacgtcggc gctatcgctc aggtcgccat tggatctacg
      361 aggagttctt agacttgcga cgttgctagc tgcaattcta gccgcagtgt cttttgctaa
      421 actctggttt ggcgaccagg gaacactcgt gatcgcattt ttaaccggac tctttgagct
      481 ccatggaata actctcgcaa ccgctacaat gttcaatcac caaatactag atctgcaagg
      541 cgccaaaacc gccattctgc tgggcactgg tgcgagtttc gccgcaaaaa ttgctatctc
      601 ctgggggctg agtactcaac cgttcgcacg aacgctcaca ttggcttttt ttccaatgct
      661 tgcgattgct tttttattcg gattgctgcc attcttaatg cattaaaatc agaataaagg
      721 agctcctatg aaaaatcttt ttgcgataaa aaaacactcg ctgagtgtag acatcgcact
      781 cacgctggtt cgcgtggtaa tgggttacgc tttcgttttg cacggttggg gaaaaatcca
      841 aactcccttt agctggatgg ggcccgaatc accggtgccc ggagtgtttc aggcgctcgc
      901 agcactttcg gagtttggcg gcggtattgg tttgattctt ggcctgctca ctacgctctc
      961 tacctttggc ttaacctgca caatgttggt ggcagcagcc atgcataagt tcgtcttcgg
     1021 cgacccgttc gtggcaaccg gcaaaggtgg cggctcttac gaactcgctg caatttattt
     1081 ggtactttcc gtattacttt tcgttgcggg tcccggtcgt ttttcggctg accgtacgct
     1141 attcggaaac aaaaactaag ttattactgt ggaacgcaaa gtgcgaacgc cttgttgttg
     1201 gcgttcgcac tcgcgcagcg aactagccaa taacgtgacg gcagagagtt cgaacaagtt
     1261 gtcgctgtag cagaggcagc aggacaagac gctgccattc gattgcttcc acccgtaata
     1321 cagtcccctc cgcccgaaag agcgtaatgt ccttcaagac aatccactac caggctcgta
     1381 acattcgccg ctaccgtccg aagctgatag ttcagtgcgc cggcgggccc ctggggaccg
     1441 gtggggcctt gtggtcccgt cgctccattc gcaccagccg gaccctgcgg accggttggg
     1501 cccactaatc cactgctggg gccgatccag ttgccatttg catcgacaac gggaactccg
     1561 ccctgcgaga ccagcgctcc agtgccattg aattgcgcaa aattttcgga gtctaatcca
     1621 tcgagcgtgt cggcactcag tgcaaaaaag accgcgctca ttggaactcg cggtgtcagg
     1681 gtttcaaaaa cagttccgtt gtgaatttct agttcgattt ctacgctagt gccaggcgcc
     1741 aatcccgcag ttaacgcaga acgaattgga agcgaagcaa gcgggagcgc agttccgttc
     1801 agaggatcga gcgccgcagc gccttgttgg tcggcaccta aactcactga atagcttccc
     1861 gcgttaacct gaaccgcgga ataacgagca aaccacacac gactaccacc cacatataac
     1921 ccaaaacgca tttctcgcgc gccgtgtgct ggggttccat tggaatccgt gagctgcccc
     1981 tggaaatgaa ttgtttgtag tccggtttct gcggcgtagg tgaaattcgc cactaaaagt
     2041 gcgaaaaaaa acaccgcgaa ggtccagggc ttgttcggtt ttaatcttag tttgttcata
     2101 gcctaattta ctgaatctgc ctttctgggc ggctccccac gatcacacca tttacccgaa
     2161 aactcggaga actaagggtt tgagcttgtc ccccagagag tccaccgaat tgcgtgttcg
     2221 tccctgtcgt tgtcggcgtg gatccggagc caccagacgg ttcgctcaca ggagtagcag
     2281 tcggcaccgg gaccttgcct tttaaactgt cgactaatcc gttgattcgg tctgagaagc
     2341 gacaccccga cacctgggcc gccacaacca cgatcaaacc caaccgaata gaattcacga
     2401 accgcataat caccctcggg gcgcccaatc gcgcccccct tattctcagg ttattgcaag
     2461 gcatgtgcta cgcaatcggt aatttatgct catgatatca gttagttaat ttttgatccc
     2521 cggtgtcttt ctgaaactgg cgtcattgtg acccaggtcc tagtggtatt acccctctcc
     2581 aataagcact ccagtcctct atagaaacgg tggaagtctg atgctatctt aggtccatct
     2641 tttcccacga ggtaacatct gtgacgtttt acaaagtccg gcgcccagta ttcgccactg
     2701 cattaatgct cttcaccgcg tcaacctctg ccacagccaa agtgtggagt ggtggtcaaa
     2761 aaaacaatgg aatggaacgt ttgcccaaac gcgcgcaaga aatattgcgg gaactacctg
     2821 gtgggcaaat cactctaaac ttcgtgctcg agcgagccac cgaagcatcg gattcgtttc
     2881 gggccgcaag tgcgggggtg caggcgctag acgcaagtta tcatcgttcg cgggctgcta
     2941 cggattttca atatttctta cgtggtactc gttcgctgga ccggcgagag gcgacaaatt
     3001 cttttagcac aacttccaat gaagaaaata aattcactct aggcgtatca cgctctttcg
     3061 aaaccggcac cgacttgagt ggcgaaattt cccagtccca aaccaaactc ggtttcgcta
     3121 cgatcgcgcc catcgatgaa accgag
//
LOCUS       DAOMXO010000223         3144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17287_length_3144_cov_5.508255, whole genome shotgun sequence.
ACCESSION   DAOMXO010000223 DAOMXO010000000
VERSION     DAOMXO010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17287_length_3144_cov_5.508255"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="PLH57_05665"
     CDS             complement(<1..1735)
                     /locus_tag="PLH57_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRK02134.1"
                     /translation="MTQSWNFPIRYKILGVLSVVVCAAVAVYLYLASTLFFEDKTLLI
                     YELNQSNVRVLANEMDTHLKNLLKQSQLIGEITLKQENIAAFDRIKDDDLVAVRVENN
                     GGELVFEKYWTDGLEAFNFSKEKIAALFSDPFPKENEHSRIQFRTYGIQASDALTPPL
                     LLTRNKLADVQVFLVSKADSLVSAFTRSGIAEVYVFDANGDVLFGSNSEINQKKDPLY
                     SASRVEQLRSEVRQFDLGGKQYLGSWYRLQSAPLVVASRVELGHAFAAARLLIRKSII
                     YAVIIVTVAFMVALIFSQSLTEPIHRMMEATYRIAKGDFNSTIHVRSHDELAVLAASF
                     NAMTVDLRSSRSQIEEYSRDLEKKVIDRTAMLETQNAAIREAQEALLRTTRLASVGEV
                     AGRAAHEVLNPLTNIIARLEKRSLEIAKNKNESLVVAEEILAAWKKAYDAGGIDSLCR
                     DLGSPSSAYPGQKMIDEDLKNIEFLVEKAKADYAVWRDDIEFLNRESLRISKIVNGMR
                     SLTRVSGNRRKMSVVEAVEMALNANHDVIKRANIQYSLEKISNPSIVADRDELVQVLS
                     NLLRNSMQSLQD"
     gene            1925..>3144
                     /locus_tag="PLH57_05670"
     CDS             1925..>3144
                     /locus_tag="PLH57_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02135.1"
                     /translation="MLLYDLGDYENRRNPQSEKVAELKAAQNDVRIRARGTIAWDNGN
                     SGDALHHLDSVLTLENANTTINFQEGGSLTVAANSLVTIEKADTRSHPTIELAQGRLS
                     KSDSSLALKNIRIKVGDTYFSEDQEGASFSLIKDGNNARIAVTKGRITSDTGHTLGAG
                     EQAEISESGLKKTDAIDALISLDKTPNHLVTNESSSEFQLEWALNSRDSRLIAKPLMK
                     LTITRNGETILEKDTYEFSERLLLESDNNYEIQLRTNDPLLKETIASKAIRVHKIKEA
                     NDLRSADQYFAGERIDFRWLVEPNIPKEAVEYKIELAHDEDFERPLHVPSSQWLSQSQ
                     SSGSLIFHRPRTVFWRVRAQSLSSPSQSIISDVRKIEIKPKPQLPAPRVKEPRIRIRK
                     IKRKLPTPETFSAA"
BASE COUNT          903 a          745 c          663 g          833 t
ORIGIN      
        1 aatcttgcaa agactgcata gagtttctta acagattact caacacctgt acgagttcgt
       61 cgcggtccgc cactatgctt ggattggata ttttctccaa agagtactga atgtttgccc
      121 gtttaattac gtcgtggttg gcatttaaag ccatctcaac ggcctcgacc acagacattt
      181 tccgtctatt gcccgaaact cgggtcaaag atcgcattcc atttactatt ttcgatattc
      241 tcaaggattc cctattgagg aattcaatgt cgtcgcgcca cactgcatag tcggctttgg
      301 ctttttccac tagaaattct atgttcttga gatcttcgtc gatcatcttt tgaccaggat
      361 aagcgctgct ggggctacct agatcccgac ataacgaatc aatcccccct gcgtcgtacg
      421 cctttttcca ggccgcaaga atttcttcgg caacaactaa agactcgttt ttatttttgg
      481 caatttccaa actacgtttt tctagtcgag caataatgtt ggtcagggga tttaacactt
      541 catgggcggc tcgtcctgct acttccccta cgctcgccaa tcgagtggtt cggagtagcg
      601 cctcttgggc ttcccgtata gcggcgtttt gcgtttccag catcgccgta cgatcaataa
      661 cctttttttc tagatctcga ctgtactctt caatctgact tcgcgacgac cgtagatcta
      721 ctgtcattgc gttaaaggaa gccgcgagga ccgcaagctc gtcgtgagag cgcacgtgaa
      781 tcgtactatt aaaatctcct tttgcgattc gataggttgc ttccatcatt ctatgaatgg
      841 gctccgtcag ggattgggaa aaaattaggg ccaccatgaa tgcaacggtt acaatgatta
      901 cggcataaat aattgattta cgtatcaaaa gcctagcagc ggcaaacgca tgccccaact
      961 ctactcggga tgccaccact aacggtgcgc tttgcagtcg ataccaagac cccaagtact
     1021 gtttcccccc aagatcgaac tgtcgcactt cggaacgcag ctgttcaact cttgaagcgg
     1081 aatagagagg atcttttttt tggttaatct ctgaatttga tccaaacaat acgtctccgt
     1141 ttgcatcgaa cacatatact tccgcaatac cggaacgagt aaatgcggat accaaactat
     1201 cggccttcga aaccaggaat acctgaacat ctgcaagttt atttcgcgtt aacaacaaag
     1261 gcggtgtcaa tgcatcgctc gcttggatac cgtaggtacg aaactgaatt ctggaatgtt
     1321 cattttcctt aggaaacgga tcggaaaaaa gtgccgctat tttttctttt gaaaaattaa
     1381 aagcctccaa cccatcggtc caatattttt caaacaccaa ctccccgccg ttattttcta
     1441 ctcgcacggc gactagatcg tcgtctttta tacgatcgaa tgcggcaata ttttcctgtt
     1501 taagagtaat ttccccaatt aactgacttt gtttgagtaa attttttaaa tgagtatcca
     1561 tttcattggc aagaactcgt acgttacttt gattcagttc atagattagc aacgttttgt
     1621 cttcgaaaaa aagcgtcgac gcgaggtaca gatacaccgc tacagcggca caaacgacca
     1681 cactcaacac cccgagaatc ttgtagcgaa ttggaaaatt ccagctctga gtcatagaat
     1741 atatatgagt acaacagact taattatgtc acaggtttcg gtgcaagcaa tgctatagga
     1801 tggcgagaaa tttccctatg gatggggaag aacattgaag gtcgatcagt cgacggaact
     1861 taagcgaccc tctccctgga gaggtgtgtt tttgtgtgca ctactcttgt gcgtggagat
     1921 ctacgtgttg ctatacgatt tgggcgacta cgaaaaccgg agaaatccac aaagcgaaaa
     1981 agtcgctgag ttaaaagcag ctcaaaacga tgttcggata cgcgctcgcg gtactattgc
     2041 ttgggacaat ggaaattctg gcgacgctct tcatcacctt gactcggtac tcacccttga
     2101 aaatgcgaat acaacaatca atttccaaga gggcgggtct ttaacagtag ctgctaacag
     2161 cctagtaact attgagaaag ctgatactcg gtctcaccca accattgagc tcgcgcaggg
     2221 ccgattaagc aaaagtgact caagtctggc gcttaagaac attcgtatca aagtcggtga
     2281 tacctacttt agcgaggatc aagagggtgc aagttttagt ttaattaaag atggcaacaa
     2341 tgcccggatc gcagtgacca aaggccgaat cactagtgac actggccaca cattgggcgc
     2401 cggagaacag gcggaaatta gtgaaagcgg gctgaaaaaa accgatgcaa tcgatgcgtt
     2461 gatttcattg gataaaacac caaatcacct agtcaccaac gaatctagca gcgagttcca
     2521 gttggagtgg gctctcaatt ctcgagactc tcgcttgatt gctaaaccac tgatgaagtt
     2581 aacaattacc cgtaacggtg aaacaatact ggaaaaagac acttacgaat tttccgaacg
     2641 actattactc gagtcagata ataattatga aattcaactc cgcacgaacg acccattatt
     2701 aaaagaaacc atagccagta aagcaattcg agttcacaaa attaaagaag caaacgactt
     2761 gcggtctgcc gaccaatatt tcgcaggaga acgcattgat tttcggtggt tagttgaacc
     2821 gaacattccg aaagaagcag tagaatacaa aattgaactc gcccacgacg aagattttga
     2881 acggccatta catgttcctt cctctcagtg gctctcacaa agtcaatcga gtgggagttt
     2941 gatatttcat agaccccgta cggtcttctg gcgagtaaga gcacaatctt taagttctcc
     3001 gtctcagtct ataatttctg atgtaagaaa aattgaaatc aaaccgaaac cacagttgcc
     3061 agcaccccgc gtaaaggaac cacggatacg cattcgaaaa ataaaacgga aattaccgac
     3121 gcccgaaact tttagcgccg ccca
//
LOCUS       DAOMXO010000224         3132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17384_length_3132_cov_3.956126, whole genome shotgun sequence.
ACCESSION   DAOMXO010000224 DAOMXO010000000
VERSION     DAOMXO010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17384_length_3132_cov_3.956126"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..2877)
                     /gene="infB"
                     /locus_tag="PLH57_05675"
     CDS             complement(184..2877)
                     /gene="infB"
                     /locus_tag="PLH57_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRK02136.1"
                     /translation="MAGLQDVKVYELAKELSMDSITLLDKLRSLDIEVKNHMSALGGE
                     EVRIVKEAIAKEQAATTKSTKTTKTTRTTRKSSATAAAGASTTPNAQEPSAASKKASP
                     TVIRRRAKGGTDDAAVTLAPSQPASSSQPETQPVAETEEAAVVQSAEAPIVEAQPQTE
                     KTVVSTPAPTPPPAPENLGSGLKMHTLLGAKAGGMTSARTGSILNIVKDVPKPIVTPR
                     PAPRPAGPITPGRPGGIINLGKEAIDRMAEEEQSMRKKGAPGARGDRDREPKDVNVLD
                     YRAKKELIFLPKKKKAPIGREIKKTAITKPAAHKRVIRIDNAIVISDLAQRMGVKSGE
                     LIKKLMGMGTMVTMNQTIDLDTATLLATEYGFEIENVAFQETQVIKKVEDAPDSLKPR
                     PPVVTVMGHVDHGKTSLLDAIRSEDVAAGEAGGITQHIGAYTVSLDDKKITFIDTPGH
                     EAFTVMRARGANVTDLVILVVAADDGVMPQTREALDHAKAAGVPIIVAVNKMDKPGAN
                     PEKIMKALAELDLLSEEWGGQTMFVKVSAIKKTGIKELLEAILLQAEVLDLKANPERA
                     AEGSVLEAQLDRSRGAVVDVLVKHGTLRPGDIVVAGPHVGRVRALTDDKGQPLKEALP
                     GYAAQLLGLEGVPTAGDTFNVMSSDAEARNLAQHRVEKVRAQNAAKTSKMTLEDLFSK
                     VQQGNVKELAVVLKTDVFGSTEAVRDSLLKLTTEKVKVKVLSATTGGITESDVMLASA
                     SNAIILGFNVRPETKAIKVAESEHVEIKTYAIIYELIDDVKKAMAGLLEKKAVEKYLG
                     RAEVRQTFSVPKAGTVAGCAVVDGKITRNCQVRLLRENRVIYTGKLNSLRRFKDDAKE
                     VATGFECGMGLENYNDIKQGDIIEAFTVDMVAQELDAPIAQ"
     gene            complement(2946..>3132)
                     /locus_tag="PLH57_05680"
     CDS             complement(2946..>3132)
                     /locus_tag="PLH57_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02137.1"
                     /translation="VEKGGEALMLSKDGTPLENVSKKESAHEPRDAKSMAEERLRQEM
                     AAAGISSSKPDNSGTAE"
BASE COUNT          656 a          786 c          765 g          925 t
ORIGIN      
        1 cgttagaaaa ggagtgttct taaaagcagg atcactcaaa tcccctggga tcatgagagt
       61 gagttcatct tttatcaact ctgcgaaacg tgcagtgcgg taactcgggc gctcggtgat
      121 aaacggctta tgcatttcaa atctcctata gatttgtggg gattcggacc ccaacggtta
      181 atgttactga gcaatcggtg cgtcgagctc ctgcgcgacc atatcgacgg tgaaggcctc
      241 tatgatgtca ccttgtttga tgtcattgta gttctcgaga cccattccgc attcaaaacc
      301 ggtagccact tctttggcat cgtctttaaa acggcgtaat gagttcagtt tgccagtgta
      361 gattacgcga ttttcacgca gcaatcgaac ctggcagttt ctcgtgattt tcccatcgac
      421 cactgcgcaa cctgccacag ttccggcttt cggaaccgaa aaggtttgcc gtacttcggc
      481 tcggccgaga tacttttcaa cagctttttt ctcgagcaat ccggccattg cttttttaac
      541 atcgtcgata agttcgtaaa tgatggcgta ggttttaatt tcaacgtgtt cactttcggc
      601 aaccttgatt gctttagtct caggtcgaac attgaaacct aaaataatgg cattggaagc
      661 acttgccagc atgacgtcac tctcagtgat tccgcctgtg gtggccgaga gaactttaac
      721 cttcactttt tcagtggtca gtttcaaaag gctgtctcgc actgcttcgg tggatccaaa
      781 cacgtcagtt tttagaacca cggccaattc ttttacgttg ccttgctgaa ctttgctaaa
      841 aaggtcctca agagtcattt tagaagtttt cgccgcattt tgcgcacgaa ccttttccac
      901 acgatgctgg gctaagttac gagcttcggc atcgctagac ataacattaa aagtgtcacc
      961 tgcagttgga actccttcga gcccgagcag ctgagctgcg taacctggga gcgcttcttt
     1021 taacggctgg cctttgtcgt cggtcaaagc acgaacacgt ccaacgtgcg gaccagcaac
     1081 cacgatatca ccaggtcgaa gcgttccgtg tttcacaaga acgtcaacca cggcaccgcg
     1141 ggaacgatca agttgtgctt cgagcaccga gccttccgct gcacgttcag ggtttgcttt
     1201 taagtctaac acctctgctt gtaataaaat ggcttcaagc agttctttga ttccggtttt
     1261 tttaatggca gaaaccttca cgaacattgt ttgaccaccc cattcttcag agaggaggtc
     1321 tagttcagca agggccttca tgattttttc cggattagca ccgggtttgt ccattttgtt
     1381 tactgccacg ataattggca cgcctgcggc cttggcgtgg tccaatgctt cacgagtttg
     1441 cggcataacg ccgtcgtcgg cagcgaccac taagattact aaatcggtta cgttcgcacc
     1501 acgcgcgcgc ataaccgtga acgcttcgtg gcctggggtg tcgataaaag ttattttctt
     1561 gtcgtcaagc gacacggtgt aggcaccaat atgttgggta atgcctcctg cttcacctgc
     1621 cgctacatct tccgagcgaa tggcgtctaa caacgaagtt ttaccatggt cgacgtgacc
     1681 catgaccgtt acgaccggag gccgtggctt aagggaatct ggagcatctt cgactttctt
     1741 gataacctga gtttcctgga acgcaacgtt ttcaatctca aaaccgtatt ccgttgcaag
     1801 aagggtcgca gtgtcgaggt caatggtttg gttcatcgtc accattgttc ccatgcccat
     1861 taatttttta atcagctcac cactttttac acccatacgt tgggctaaat cagagatcac
     1921 aattgcattg tcgatacgaa tgacgcgctt gtgggctgcg ggtttggtga tagcagtttt
     1981 cttaatttcg cgtccgatag gagccttctt tttcttcgga agaaagatga gttctttttt
     2041 ggctcggtaa tcaagcacat tgacgtcttt aggttcgcga tcgcgatccc cacgagcgcc
     2101 gggtgcgccc ttttttcgca tcgactgttc ttcttccgcc atgcggtcga ttgcttcttt
     2161 accaagatta atgatgccac caggacgacc gggcgtgatc ggtccagctg gacgaggcgc
     2221 aggccgcgga gtgactatcg gtttaggaac gtcttttacg atattcaaaa tgctgcctgt
     2281 tcgggccgag gtcatcccgc cggctttcgc gccaagaagg gtgtgcattt tgagtccact
     2341 tcccaaattt tctggtgccg gtggaggtgt tggagccgga gtgctgacaa ctgttttttc
     2401 ggtttgaggt tgcgcctcga ctatcggagc ttctgcactt tgaacgacag ctgcttcttc
     2461 agtttcagca acaggttgcg tttctggctg actagaactt gcgggttgcg aaggcgccaa
     2521 tgtaacagct gcgtcgtcag tgccgccttt tgcacgacgt cgaattacag ttgggcttgc
     2581 tttttttgaa gcagcagaag gttcctgcgc attcggagta gtcgaagcgc cagctgcagc
     2641 ggtcgctgat gacttgcgag tggtgcgagt ggttttggtg gtcttagtgc tttttgtggt
     2701 tgccgcttgt tcctttgcaa tcgcctcttt aacaatacga acttcttcgc cgcccagcgc
     2761 gctcatgtgg ttctttactt cgatatcgag ggatctgagc ttatccaaaa gtgtgatcga
     2821 gtccatggag agttcttttg ccagttcgta cacttttaca tcttgcaaac ctgccataga
     2881 agcggctccc ttttttgaat cgttttcaga gcttgttgtt tgtgtcactg cattccacct
     2941 aatttctatt cagcggtccc cgaattatcc ggtttcgaac tgctgattcc ggctgctgcc
     3001 atttcttgtc tcaagcgctc ttcagccatc gatttggcat ctcgtggttc gtgggcactt
     3061 tcttttttag aaacgttctc gagtggggtg ccatctttgc tgagcatgag agcttcgcca
     3121 cctttttcca cc
//
LOCUS       DAOMXO010000225         3121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17465_length_3121_cov_4.060665, whole genome shotgun sequence.
ACCESSION   DAOMXO010000225 DAOMXO010000000
VERSION     DAOMXO010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17465_length_3121_cov_4.060665"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..676)
                     /locus_tag="PLH57_05685"
     CDS             complement(14..676)
                     /locus_tag="PLH57_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRK02138.1"
                     /translation="MPNVSLKIVFENENVVLVDKPAGWLSRPSRFGLRDERPCVSSEL
                     AKTRSELWVVHRLDIDVSGLLLFAKNKSSHRALSQAWEAHEVQKKYHALAHRLVAATT
                     VEPEIGELETWEHLIAEGKRRSFLAEHGKKAITRAICVKVENRVHHWLLEPVTGRRHQ
                     LRVQMVARGFVILGDVLYGGANEQWKSGIALRAVRLDFKKLDLSKDLKIPDHFEIESL
                     SL"
     gene            702..1646
                     /locus_tag="PLH57_05690"
     CDS             702..1646
                     /locus_tag="PLH57_05690"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRK02139.1"
                     /translation="MTNENFAPQQTIIEILKTKPRTVSAEIIPPRNGMETQTIFNQIE
                     RLRGAGVDFIAVTKRAGGSLRGGTLPLAQLVRSSFGVCSLAHFTCRDYTKEEIENNLM
                     DHHYFGIRNILALRGDPPDGQPGYFQPSPNRHTYAYQLVEQIRALNAGRYLHRDGFDD
                     KNKERTGQPTKFCIGVAAHPEHAPLEQGVEYFQRKIEAGAQYGITQMLFSADPYKRFV
                     EALAKRGLNVPILPGVRIITSAQTAERMRNKFSDVSIPDSLIEKLTKAHSKEAAREVG
                     MNATLELCQELLKAGAPGIHIFVMSDTGSACELISALR"
     gene            complement(1661..>3121)
                     /locus_tag="PLH57_05695"
     CDS             complement(1661..>3121)
                     /locus_tag="PLH57_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02140.1"
                     /translation="ATSNPEGLVVLLDEFPRAGGDNPFVRKEIQQAFLSILDEGVLPA
                     IPWETKTVGSKHVRENSKTPLPKATLFYATGNMIEGVLGPNAEFMTNNELTAQLLWLQ
                     KHPERFRSVYQQSFVAALRSRMGEPNVFMNLSDADLEWLRDEKFLPNALKLLKEEGVE
                     VTLAKSAKDYFRKEQIAIRGGRWVRDKVEVLIEAPLNAWLLGVLKYRNSQVTVEFDAD
                     SREMRASVARGGKVYEEKVLAKVPRARSDVHPEVKQKLRISTHVHEAGHAVVMRALFG
                     KDAVDRIVTFGGGDGGWTEFATDAKGHEPYLTTKGGPFHIAALLGGHLAEQLFQKGNT
                     SNGASSDFSRARSTSLTMILDGSVLSEAPIAITVDPKNGQLQLSDAMKLELELKQKAL
                     LGFGHRLATWILKHNSKLIAAIAERMEANHQNFSIEREELEEIIEKTGLRVPSDKEIR
                     KIMLADRKTHRTCREMLLNEDTTVLDRLLDFILRRK"
BASE COUNT          766 a          838 c          712 g          805 t
ORIGIN      
        1 cacctaaacg aattcataga gatagactct ctatttcaaa atgatcagga atttttaggt
       61 cttttgataa atctaacttt ttaaaatcta gccgtaccgc gcgcagggcg attccacttt
      121 tccattgttc gttagccccc ccgtagagca catcgccgag aattacaaac cctcttgcca
      181 ccatctgcac tcgcagctga tgacgtcgcc cagtaacagg ctcgagcagc cagtggtgaa
      241 ctcggttttc cactttaacg caaatggccc gagtgatcgc tttttttccg tgctctgcta
      301 gaaacgagcg acgcttccct tcggctatta aatgctccca ggtttctagc tctccaattt
      361 cgggttctac ggtggtagcg gccaccaagc gatgggcaag ggcatggtat tttttttgta
      421 cctcatgggc ctcccacgcc tggctcaagg cgcgatggga actcttgttt tttgcaaaca
      481 acaaaaggcc cgaaacgtcg atatccaagc ggtgaacgac ccatagctcg cttcgagtct
      541 tggctagctc gcttgaaacg caggggcgtt catctctaag cccaaatcgt gaggggcgcg
      601 agagccagcc tgccggtttg tcgaccagca ccacgttttc gttttcaaat acgatcttga
      661 gagatacgtt cggcataggg tataaaagag actcttttac tatgacaaat gagaacttcg
      721 cgccacaaca aaccattatc gagattctaa aaaccaaacc gcgtacggtt tcagctgaaa
      781 tcattccgcc tcgcaatgga atggaaactc agacgatttt taatcaaatc gaacgcctac
      841 gtggcgccgg ggtagatttt attgcagtca ccaagcgcgc gggtggtagt ctgcgcggtg
      901 gaaccctgcc gttggctcaa ctcgttcgtt cgagctttgg cgtgtgttcg cttgcccatt
      961 tcacctgccg cgattacaca aaagaggaaa tcgaaaacaa tttgatggac catcactatt
     1021 tcggaattcg aaatattttg gccctgcgag gcgacccgcc cgacgggcag cccgggtatt
     1081 ttcaaccttc acccaatcgg cacacctatg cctatcaatt ggtagaacaa atccgagcgc
     1141 ttaacgctgg ccgttacctt caccgtgacg gatttgacga caaaaacaaa gaacgcacgg
     1201 gacaacctac gaagttttgt attggtgtcg ccgctcatcc ggaacacgca ccgctggagc
     1261 agggtgttga atattttcaa agaaaaattg aggcgggcgc gcaatacggc attacccaga
     1321 tgttattttc agcggaccct tacaaaagat ttgtcgaggc attggcaaag cgtggtctaa
     1381 acgtgcccat acttcctggc gttagaatca taacatcggc tcagaccgcc gaacgcatga
     1441 gaaacaagtt ttcagatgtt agcattcctg attcgctgat cgagaaactg accaaagctc
     1501 attccaaaga agcggcgcgc gaggtgggca tgaatgcgac tctcgaactc tgccaggaac
     1561 tcctcaaagc cggcgcccct ggcatacata tttttgtgat gagcgacacg ggctctgcct
     1621 gcgaactaat ctcggccctt cgttaacgtc gaaagcagcg ctattttcgt cgcaaaataa
     1681 aatcgagcaa acgatcaagc accgtggtgt cttcgttcaa taacatttcg cgacaagtac
     1741 ggtgtgtctt gcggtcggct aacattattt ttcgtatttc tttgtccgaa ggcactcgca
     1801 aaccggtttt ttcaataatt tcttctagct cttcgcgttc gattgaaaag ttttgatgat
     1861 tggcttccat tcgctctgca atcgcagcaa tgagcttcga gttgtgtttc aagatccagg
     1921 tagccaaacg atgaccaaat ccaagaagtg ccttctgttt aagctccaac tctaatttca
     1981 tcgcgtcgga caactgtagt tgtccgtttt tcggatcaac cgtaattgcg atcggcgcct
     2041 cactgagaac actgccatct agaatcatcg tgagcgaggt ggaccgtgct cgtgaaaaat
     2101 ccgaactcgc accgtttgac gtattacctt tttgaaataa ctgttcggct aaatgtcccc
     2161 caaggagtgc cgcaatgtga aatggcccac ctttggttgt taaatacggt tcatggccct
     2221 tggcatcggt ggcaaactca gtccaaccac catcaccgcc gccaaatgtc acgatgcgat
     2281 caaccgcgtc ttttccaaaa agcgcccgca tcaccactgc gtgacccgcc tcgtgaacat
     2341 gagtggatat acgtagcttt tgtttaacct cggggtgcac atcacttcgt gcgcggggta
     2401 ccttggctaa cactttttct tcgtacactt tgccgccgcg ggccacgctg gcccgcatct
     2461 cacgactatc ggcatcgaac tctaccgtca cctgcgagtt gcgatactta agcacaccaa
     2521 gcaaccacgc gttgagtggc gcttcaatta acacttcgac tttgtcgcgc acccagcgac
     2581 caccccgaat agcgatttgt tcctttcgaa agtaatcttt cgcacttttg gctagggtca
     2641 cttcgacccc ttcttccttg agcaatttca acgcattcgg tagaaacttt tcgtcacgca
     2701 accattctag atcggcatcg cttaaattca tgaacacgtt cggttcgccc atgcgactcc
     2761 gtaacgcggc gacaaacgac tgttgataca ccgaccgaaa ccgttcgggg tgtttctgca
     2821 accaaagtaa ttgagccgtc agttcgttat tggtcataaa ctcagcatta ggccccaata
     2881 caccctcgat catattgccg gtggcgtaaa ataacgtggc cttcggcaaa ggagttttgg
     2941 agttttctct cacatgctta gatcctacgg ttttggtttc ccatggaatt gcgggaagca
     3001 ctccttcgtc taagatgctc aaaaacgctt gctgaatttc ttttcgaaca aaggggttat
     3061 cgccgcccgc tcttggaaac tcgtcaagaa gaaccactag tccttccgga ttactggtag
     3121 c
//
LOCUS       DAOMXO010000226         3109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17565_length_3109_cov_5.183039, whole genome shotgun sequence.
ACCESSION   DAOMXO010000226 DAOMXO010000000
VERSION     DAOMXO010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17565_length_3109_cov_5.183039"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLH57_05700"
     CDS             complement(<1..741)
                     /locus_tag="PLH57_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02141.1"
                     /translation="MKRGLGLIKCVGLFLALVVFNLAEARTPDARIVDVRATSSVAGM
                     SFDLVFSQPYSAKNIAVSKERNFVQAVAKNAKMNSGKTLALRVPELEKVFAYPFAKNT
                     SRIRIIARGQEPPEGTVSVWNTSNNVVRIFLKKNAPSEVVQTSKRIDSAAGGPAVNSE
                     KVVTRDAALAEVLAKTAEIDLQNPDSVVKPKNETPITNTTDVSSIDKQERKQVGTKSD
                     PSRHFLRMFLVLGGVLGLFGAGVFLLKRY"
     gene            complement(751..1185)
                     /gene="fliN"
                     /locus_tag="PLH57_05705"
     CDS             complement(751..1185)
                     /gene="fliN"
                     /locus_tag="PLH57_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRK02142.1"
                     /translation="MADQAAEQAAMNNDAAAIASAMGGAADAPAGDAGGAAAGGSGEA
                     GAPVAPKKQSDRAPVQTLDFILDIPLRVTCELGRTKMLIRDILQLAQGSVIELSKFAG
                     EPLEVLVNEKLVARGEVVVVNEKFGIRLTDIISPVERIEQLK"
     gene            complement(1201..2220)
                     /gene="fliM"
                     /locus_tag="PLH57_05710"
     CDS             complement(1201..2220)
                     /gene="fliM"
                     /locus_tag="PLH57_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRK02143.1"
                     /translation="MNQVLTQSEVDALLSAVSDGNVDLGANPGADQTGAVQGGGGGSE
                     PGEPEIAAYDLTNQDRVIRGRMPTLDIIYERFIRLFRISLSNSLRKIASISIISTDLL
                     KFGEFVNTLPIPSCMSIMRFEALRGPALLVFESKLAYALVDSYFGGTDRPYTKIEGKE
                     FTHIELSIIKKVMDLAISDLEEAWAPVHKTRISFVRTEVNPQFVGVVPPSDVIISTTF
                     EVELENASGTIALVIPYSTIEPIKQKLNATFQAETDSKDKLWTKAMSEHLQNVESEVV
                     VNLGETTITVGDLVNLNIGDIIPLTQDADGELNLQLEGVGKFRCLFGVSRGNRAVQIT
                     RPVLD"
     gene            complement(2230..2823)
                     /locus_tag="PLH57_05715"
     CDS             complement(2230..2823)
                     /locus_tag="PLH57_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRK02144.1"
                     /translation="MAEQAETSAAGPAPSGGGGSKLVMIVALVNLIATLGVIAVLFLA
                     FQKEKAKPTVEDMVLDDKGHGAAASGGGEAKKDDHGAGGKDSKAAPAVSDAGKIMALE
                     PFTVNLSSGLGTSPRYVRMNVSVELEPGVPDAEFNIKLPRVRDTVINLLNSKKATELN
                     APEGREQLKDDIRRAVNSFMLQSKIKGVYFTNFAISN"
     gene            2905..>3109
                     /locus_tag="PLH57_05720"
     CDS             2905..>3109
                     /locus_tag="PLH57_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02145.1"
                     /translation="MPTTEQFAEQLETIYRANRIRRAAYVVGLAKTLTLRDIQASKQH
                     KTATEFTVEYFRIFGRTCSLRSYE"
BASE COUNT          745 a          837 c          672 g          855 t
ORIGIN      
        1 ataccgtttt aatagaaata ctccggcgcc gaacagtccc aataccccgc ccaacactaa
       61 aaacattctc agaaagtggc gactaggatc gcttttggta cccacctgtt tccgttcttg
      121 tttatcgatg gaagacacgt cggtggtatt ggtaatgggc gtctcgttct ttggttttac
      181 gaccgagtct ggattttgaa gatcaatctc tgcggttttt gctaaaactt cagcaagcgc
      241 tgcatcgcgc gtaaccactt tttcggagtt tactgcaggg ccacccgcgg cactgtcaat
      301 tcttttgcta gtctgaacga cttccgatgg ggcatttttc ttgagaaata tgcgcaccac
      361 attgtttgag gtattccaca cgctcacggt accttcgggg ggttcctgac cgcgggcaat
      421 aattcgaata cgactggtgt ttttagcaaa tggataagca aacacttttt cgagctccgg
      481 aacccgcaaa gctagcgttt ttccagagtt catcttcgcg ttcttggcta cggcttgcac
      541 gaagttacgt tccttactaa ctgcaatatt tttggcggaa tatggctgac taaatactaa
      601 atcaaaagac atgcccgcta ccgaagacgt cgctcgaaca tccacgattc gtgcatctgg
      661 agtgcgggct tcggctaaat tgaaaactac tagcgctaga aacagaccaa cacatttaat
      721 caagcccaaa ccccgtttca tgactcacct ttatttcaac tgttcgatac gttcgacggg
      781 cgaaataata tccgtcaatc gaatgccaaa cttttcgttc accacaacta cttctccacg
      841 agcaactaac ttttcgttta ctagtacctc gagtggttcg cccgcaaact tcgaaagctc
      901 gatcactgag ccttgcgcta actgcaaaat gtcgcgaatg agcattttag tgcgacctaa
      961 ttcgcaggta acccgtagtg gaatgtcgag aataaaatcg agggtttgaa ctggggctcg
     1021 atcgctttgt tttttgggtg caaccggagc tcccgcctcg ccgcttcctc cggctgctgc
     1081 tccgccagca tctcccgccg gagcatctgc cgctccgccc attgccgaag ctatcgccgc
     1141 cgcatcgtta ttcattgccg cttgttctgc tgcttgatcc gccattttta atctcccttt
     1201 ttaatccaaa accggccgcg taatctgaac cgcccgattg cctctagaaa caccaaataa
     1261 acatctgaac ttccctactc cctcgagctg aagattgagt tcgccatcgg catcttgagt
     1321 gagtggaata atatcgccga tatttaaatt tactaaatca ccaacagtaa ttgtagtttc
     1381 tccgagattc actacgacct cggattcaac attctgaaga tgctcactca tggccttggt
     1441 ccaaagtttg tctttgctgt cggtctcagc ttgaaacgta gcgtttaatt tttgtttgat
     1501 tggttcgatt gttgagtagg gaattaccag tgcgatcgtt ccactagcat tttccagttc
     1561 aacttcgaaa gttgtcgaaa tgatcacgtc actcggcggt accacgccca cgaattgcgg
     1621 attcacttcg gttcgcacga aactgatgcg agtcttatgt accggcgccc aagcctcttc
     1681 caggtcactg atcgcaaggt ccataacttt cttgatgatc gagagttcaa tgtgggtgaa
     1741 ctcttttcct tcgatcttag tgtatgggcg atcggtccca ccaaaatagg aatcgaccaa
     1801 agcgtaggcc agtttacttt caaataccaa aagcgctggc cctcgtagtg cctcgaatcg
     1861 catgatactc atgcacgacg ggatcggcag agtgttcacg aactcgccaa acttcaataa
     1921 gtctgttgaa ataattgaga tactggcgat ttttcgcaaa ctattactta aactaatccg
     1981 aaacaaacgt atgaatcgct cgtatatgat atcgagcgtt ggcatgcgtc cgcgaattac
     2041 gcggtcttga tttgttaaat cataagcggc aatttccggc tcaccgggct cactaccccc
     2101 gccgccgcct tgaaccgcgc cggtttgatc agcgcctgga tttgcgccaa gatcgacgtt
     2161 cccgtcgctc acggcactta gtagcgcatc gacttcactt tgtgtgagga cttgattcac
     2221 actggcctcc taattgctga tcgcaaagtt ggtgaaatac acacctttaa ttttgctttg
     2281 cagcataaaa ctattcactg ctcgccggat atcgtctttt aactgttcgc gtccttccgg
     2341 tgcgtttaac tcagtagctt tttttgaatt caaaagattg attaccgtgt ctcttacgcg
     2401 cggtagctta atgttaaact ccgcgtcagg aacccccggt tctagctcta ccgacacgtt
     2461 catgcgcaca taacgagggc tggtgcctag accactagat aagtttacgg taaatggctc
     2521 gagcgccata atcttaccag catcggaaac cgctggtgcg gctttcgagt cttttccacc
     2581 ggcgccgtgg tcgtctttct tggcttcgcc cccaccactc gcagctgcgc catgaccttt
     2641 gtcatccaac accatgtctt cgaccgttgg tttggctttc tctttttgaa acgccagaaa
     2701 taacactgcg attacgccca aggtggcaat taagtttact aacgccacga tcattacgag
     2761 cttactaccg ccgccaccac tcggtgccgg tcctgctgct gatgtttctg cttgctctgc
     2821 catggtaagc ccctttcgcc cggagctaag aaaagcaaag catatgccat gcgttgtgag
     2881 cagccactct atgcgcggca acacttgcca actacggagc aattcgccga acaactagaa
     2941 acaatttacc gcgccaatcg aattagacgt gcagcctatg tcgtgggcct agccaaaact
     3001 ttgactctcc gtgatatcca agcgtcaaaa caacacaaaa ccgcaaccga atttacggtc
     3061 gagtattttc gaattttcgg cagaacttgc agtctacgct cttatgaga
//
LOCUS       DAOMXO010000227         3105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17600_length_3105_cov_3.255410, whole genome shotgun sequence.
ACCESSION   DAOMXO010000227 DAOMXO010000000
VERSION     DAOMXO010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17600_length_3105_cov_3.255410"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLH57_05725"
     CDS             <1..820
                     /locus_tag="PLH57_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRK02146.1"
                     /translation="PSNTPPPEELPPPTLDDVIPFIPDIPPEVIFKDLLVGTPEDAAK
                     ATEHAGHMVKKILEDSNTSAAEAVANASYEHANSVAVYSVLFAMGIGVKDKSLLQDFV
                     TASLAHDVGLSQCPPSVCKVPVLQHAGAQKNQYETHVARALQLVDELGFVVSSRARKI
                     IEQHHEKFDGSGYPAHLEGFHISDHAQILGLAELIDSMNRGFYDGKKYTLNDSIALVC
                     KIEKQTTFPVYFNPDIFKKVMLWIRKGSGLDFMAQAASVVDETRAQILDSKPAA"
     gene            complement(836..>3105)
                     /locus_tag="PLH57_05730"
     CDS             complement(836..>3105)
                     /locus_tag="PLH57_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02147.1"
                     /translation="PSKGKWLNVGKNSEGGSQWRFMDPETAAKVEEAQKKGLPLDRAL
                     EAAGSKKNGWEWEGDQPQHHNGDWNFASEKPNLTCYNQDGTKSHSKVETSPSGELIAS
                     EDSQKALAKVAQMEAQAEADKQKRKKSLFGSAESGEPEINEKSGEHGDAKSKNDTDPS
                     SETSGAQEVALPNKKNSQLGDGGPKKNEADFRDSASDSKKGDLRTSNKSESDQGAELG
                     ATESKKESAGINQKLFDAKKQEKDSPKTGSSAASKTGAIKEKNSEANDIERKSSGEAG
                     TTSKETQTNAKEKTRSPFLPKAKTDASESVNNDKSSKADQNTKNKNRENPFLPGAKKP
                     AGVEGPNAEARKKNQQLELAEAKRSEREALDSEDEKKGDAGESLTGENAKKKSAFANA
                     GNRDSKKRRDEESRKDTSSEKNQNGGSSSFTDSSEKKDKPGENSEQKKDETEGGWSHH
                     KETGEKKKKWSGRDSDQGKLEPDASDKLVESELSGQDIKVKRASEEIFEYPHDHFGVL
                     NGTWERVEDVGGDWFIYVDPAIRSKKDYNLHVVKNWWLYRGPARPAYLAPIKIWQFRK
                     VRPEKLDAFEKLEAPIRSFLEGLLPGAKKEKIKRADEGLEKKQFRFVAPMGVAISISE
                     FSQDATITPEKVVEFFCQMVGQSLKGASVAVFRNDGGKPRFLSDASDFQRIVAEKCSG
                     ERRVFLEKQGTHACLAVVVFDSQKKNILGGVLAETHVDQAEELNEQSNYLAHASFGIR
                     GCLIDEALVAFKKAA"
BASE COUNT          662 a          837 c          640 g          966 t
ORIGIN      
        1 cccctcaaac accccccccc ccgaagagtt gccgccaccc acactggatg acgtcattcc
       61 gttcataccc gacattccac cggaagtgat ttttaaagac cttctagttg gcactcctga
      121 agacgcggca aaggctacgg aacacgctgg acacatggta aagaaaatcc tggaagactc
      181 aaacacttcc gccgccgagg cagtggcgaa tgcctcttat gaacacgcca acagcgtggc
      241 ggtgtacagc gtgctttttg cgatgggaat tggggttaaa gacaaatctt tactgcaaga
      301 cttcgtaacc gcttcattgg ctcacgacgt tggcctctcg caatgcccgc caagcgtctg
      361 caaagttcca gtactccaac acgccggggc tcagaagaat caatatgaaa cccacgtggc
      421 ccgcgctctt cagctagtcg acgaacttgg atttgtcgtt tcttcgcgcg cacgcaagat
      481 cattgaacaa catcatgaaa aattcgatgg atcgggctat cctgcgcacc ttgagggttt
      541 tcacatctca gaccatgcgc agattctggg actggcagaa ttgatcgaca gcatgaatcg
      601 agggttctac gacggaaaaa aatataccct gaacgattct atcgcgctcg tttgcaagat
      661 cgaaaaacaa actacatttc cggtctactt taacccggat atatttaaga aagttatgct
      721 gtggatacgt aaaggtagcg gtctagattt catggctcaa gccgcttcgg tggtcgacga
      781 aacacgcgca caaattctcg actccaaacc agctgcctaa aacacagggt ccacgttaag
      841 ccgctttttt aaacgcaact agcgcttcat ctattaaaca gccgcgaatg ccaaaactgg
      901 cgtgcgctaa atagttcgac tgttcgttga gttcttctgc ttgatcgaca tgcgtttcgg
      961 caagcacccc accaagaata tttttctttt gagaatcgaa cactacgact gctaaacacg
     1021 catgggttcc ctgtttctcc aaaaacaccc gccgttctcc cgaacacttc tccgccacta
     1081 ttcgctgaaa atcagaagca tcggacaaga atcgtggttt tccgccgtca ttgcgaaata
     1141 ctgccacgct agctcctttt aaggactgtc ctaccatttg acaaaaaaac tccacgacct
     1201 tttccggggt tatcgtcgcg tcttgactga attccgaaat agaaatggcg acgcccattg
     1261 gggcgacaaa ccgaaactgt ttcttttcta aaccttcatc agctctctta attttttctt
     1321 tttttgcgcc gggcaatagc ccttccaaaa aactgcgaat tggagcctca agtttttcga
     1381 acgcgtccaa tttttccggt cgtacttttc taaattgcca gatctttatt ggcgccaaat
     1441 aagctggccg agcaggaccc cgatagagcc accaattttt gactacgtgt agattgtaat
     1501 ccttcttact tcgaatagcc gggtctacat aaataaacca atccccacca acgtcttcca
     1561 ctctttccca ggttccgttg agaactccga agtgatcgtg gggatactcg aaaatttctt
     1621 cgctagctct ttttaccttt atatcttggc cgctcaattc gctctcgact aacttatctg
     1681 acgcatcagg ttccaattta ccctggtccg aatctcgccc gctccatttt ttctttttct
     1741 caccagtttc tttatgatga ctccagccac cttcagtttc atcttttttc tgttcagagt
     1801 tctcgcccgg tttatctttc ttttctgaac tatctgtgaa actcgaactt ccaccgtttt
     1861 ggtttttttc gctcgaagta tctttccgac tttcttcatc gcggcgtttt ttggaatctc
     1921 gatttccggc attcgcaaat gcactctttt tcttagcatt ctcaccggtc aaactctcgc
     1981 cagcgtcgcc ttttttttca tcttccgaat ctaaggcttc ccgttctgag cgtttcgcct
     2041 ccgctaactc caactgctga tttttttttc tggcttccgc atttgggccc tccaccccgg
     2101 cgggtttctt ggcgcccggc aaaaatggat tttctcggtt tttatttttg gtgttttgat
     2161 cggctttgga agacttatcg ttatttacac tctcgctcgc gtcggtctta gccttaggca
     2221 gaaacggcga acgtgtcttt tctttggcgt tggtttgggt ttccttcgat gtagttccag
     2281 cctcaccact acttttacgt tcgatgtcgt tagcttcgga gtttttttct ttgatggcac
     2341 cggtttttga agcagcactt gacccagttt tcggtgaatc tttttcttgt tttttggcgt
     2401 caaaaagttt ttggtttatt ccggccgact cttttttgga ctcagttgct cctagctccg
     2461 caccctgatc tgactcagac ttatttgaag ttcgaaggtc tccctttttt gagtcagaag
     2521 ctgagtctcg gaaatctgct tcattctttt tcgggccacc gtcccctaat tgggagtttt
     2581 ttttgttcgg aagcgctact tcttgcgcgc cagaggtctc actagagggg tctgtgtcgt
     2641 ttttagattt ggcatcgcca tgctcgccgc ttttttcgtt gatttcgggt tcgcctgatt
     2701 cagcggaccc aaataaactt tttttccgtt tctgtttatc agcctcggcc tgagcttcca
     2761 tctgagctac tttggccaat gccttctggc tgtcttcaga cgcaattagc tcacccgatg
     2821 gactagtttc cactttacta tgactcttgg tgccgtcttg attataacaa gtgagatttg
     2881 gtttctctga ggcaaagttc caatctccat tatggtgttg gggctgatcg ccttcccatt
     2941 cccaaccatt ttttttactt ccggcagctt caagcgcccg gtcgagtggc aggccttttt
     3001 tttgcgcttc ttcgacctta gctgcggttt cggggtccat aaaacgccac tgacttccgc
     3061 cctcgctatt ttttccgacg tttaaccact tacctttgga cgggt
//
LOCUS       DAOMXO010000228         3103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17614_length_3103_cov_3.832021, whole genome shotgun sequence.
ACCESSION   DAOMXO010000228 DAOMXO010000000
VERSION     DAOMXO010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17614_length_3103_cov_3.832021"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLH57_05735"
     CDS             <1..468
                     /locus_tag="PLH57_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02148.1"
                     /translation="ANEARNENVKSLAHLSRIILKYTQTHSVAAREFLESVASSADLF
                     RYDPELARPNLYPAHARIDLIMPSGGRIAVTMKLQLWGLIDPKSTEIQTQEVYYNHKV
                     YHSDILPKVEFTVKAYPSAMNEVPTQNEISAVAELLRQATERSFSELKAKQRI"
     gene            477..1532
                     /locus_tag="PLH57_05740"
     CDS             477..1532
                     /locus_tag="PLH57_05740"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRK02149.1"
                     /translation="MPGEFRKREIVGNQYIVTHLVDDSNDGLRIDQFVKKHYRTKSRN
                     QIQKAIAGGEIRMRLREDGQLLRPSTRLIAGDEVQVVTERFDEPNVNFGYKVLFEDDD
                     LLVLDKPANLPVHPAGRFLFNTLLTNLRKERSDWVLAGKRDFYITHRLDRETSGVIAL
                     AKNPESAAWMVRQFRERETEKRYFAICYGHVSEKLFTVDLDIGPAQGSNIRLRVGVYP
                     KGTELLRPESGAQNACTHFTLLQHGPNVSLLDCKIETGRQHQIRAHLAAAGYPVVGDK
                     LYSGDESIFLHYLDHGVLTDEMKHALGFERHALHSRYLRFFHETRGEWLEIESALPAD
                     MKRLLECGTSSRPCAKN"
     gene            1496..2185
                     /locus_tag="PLH57_05745"
     CDS             1496..2185
                     /locus_tag="PLH57_05745"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRK02150.1"
                     /translation="MRYELKTLREKLGPVELTLETIADLDRAIDSLCEGVDEKSAERI
                     FVEELCPYFGVVWPAARALGEHVARMGSWLKDQRILEVGCGLAIPSLVAAKLGARVTA
                     SDFHPEVPVFLARNQRHNELTFPYLHLDWRASQNNLGKFDFVIGSDVLYENSHPKDVA
                     RTLSSYCEKKGHIILADPGRSYLQAFLDELQGFGFRYDILIRNVLDGHNDRAGDRRTK
                     EVFVISCTSQS"
     gene            complement(2193..2693)
                     /locus_tag="PLH57_05750"
     CDS             complement(2193..2693)
                     /locus_tag="PLH57_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK02151.1"
                     /translation="MADGTLLNLSHRQGAKLLEEAVSLLHQGLLKPAKNKLELALRWQ
                     PSAEAYTHLAWVLSFEHKYQDAIALCEKAVELDCEFGNAYNDIGMYYMKLGDLDAAEP
                     WLEKAKKAARYDKRQTPYLNMGRIFLSRCQFSEALAEFEEALKYEPENIELRTVVERI
                     RLKHPR"
     gene            complement(2755..>3103)
                     /locus_tag="PLH57_05755"
     CDS             complement(2755..>3103)
                     /locus_tag="PLH57_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRK02152.1"
                     /translation="FCRSHGAIAKLSSIHVNTWFGRYDKLTCVRRVLKSFYGAVDAKT
                     LAQIAYIGDSPNDEPFFEALPFTVGVANVAPFLKKMDYAPTYITEREGSFGFAELADR
                     LIAARSKILLPKN"
BASE COUNT          813 a          760 c          769 g          761 t
ORIGIN      
        1 gcgaacgagg cccgcaacga aaatgtgaag tcactcgcac atttatctag aataattttg
       61 aagtacactc agactcattc agttgctgcc cgtgaatttt tagagagcgt ggcgagttcg
      121 gcggatttat ttcgctatga tccggaactt gcccgaccaa acctttaccc agcccatgcc
      181 cgcatcgatt tgatcatgcc cagcggaggc cgaattgcag tgaccatgaa actgcaactg
      241 tggggattaa ttgacccgaa aagcacggaa atccaaacgc aagaggttta ctacaaccac
      301 aaagtctatc attcagacat cttgcccaag gttgaattta cggtaaaagc ttatccaagt
      361 gctatgaacg aagttcccac tcagaacgaa atatctgcag tcgctgagct tttaagacaa
      421 gccaccgaac ggagtttttc ggaactaaaa gcgaaacaaa ggatctaatt ttccctatgc
      481 cgggtgaatt tcgaaagcga gagatcgtag gaaatcagta catcgtcact cacctggtcg
      541 acgacagtaa cgacggctta cggattgacc agttcgtgaa aaaacattac cgaaccaagt
      601 ctcgcaacca aatccaaaag gcaatcgccg gcggagagat acgaatgcgt ttgcgagaag
      661 atggccagct actgcggccc tcgacgcgct taattgcggg cgacgaggtc caagttgtga
      721 ccgagcggtt cgacgaacca aatgtaaatt ttgggtacaa agtactcttc gaagacgacg
      781 acttattggt actcgataaa ccggctaatt taccggttca tcccgccgga cggtttttat
      841 ttaacacttt gctcacgaac ctacgcaagg aacgcagcga ctgggttctc gccggaaaac
      901 gcgactttta tattacccat cgcttagatc gtgaaacaag cggcgtaatc gccctggcca
      961 aaaaccccga atctgccgcg tggatggtgc gccaatttcg agaacgggaa accgaaaaac
     1021 ggtacttcgc catatgttac ggtcatgtta gcgaaaaact ttttacggtt gatctcgata
     1081 ttggccccgc acaaggttca aacattcgat tgcgcgttgg cgtgtacccc aagggcacgg
     1141 agctattgag acctgagagc ggagcccaga atgcgtgcac tcattttacg ctattgcaac
     1201 acggaccgaa tgtgagtttg ctcgactgca agatcgaaac cggtcgtcag caccaaatta
     1261 gagcccacct ggcggcggca gggtacccgg tagttggcga taaactctac agcggcgacg
     1321 agtcgatttt tttacattat ttagaccacg gcgttttaac agacgaaatg aagcatgcgc
     1381 tcggattcga acgccatgcg ctccatagtc gttacttacg tttttttcat gaaacccgcg
     1441 gagaatggct agaaatagaa agtgccttac ccgccgatat gaagaggctt ttagaatgcg
     1501 gtacgagctc aagaccctgc gcgaaaaatt aggtcctgta gaactcactc tcgaaacaat
     1561 tgccgatctt gatcgtgcca ttgattctct ttgcgaaggg gtggacgaaa agtcggcgga
     1621 acgaatcttc gtagaagagc tgtgtcccta tttcggtgtg gtgtggccag ctgcgcgagc
     1681 actaggcgaa catgttgcga gaatgggaag ttggttaaaa gatcagcgga ttctagaagt
     1741 cggctgcggg ctagcgattc cttcactagt ggcagccaaa ttgggggccc gggtcaccgc
     1801 cagcgacttt cacccagaag ttccagtctt tcttgcgcgt aatcagcggc acaacgagct
     1861 gacgttcccc tacctgcacc tagactggcg cgctagtcaa aacaatctcg gcaaatttga
     1921 tttcgtaatc ggaagcgacg ttttgtatga aaattctcac ccgaaagacg tggcgcgaac
     1981 gttaagcagc tattgcgaaa aaaagggaca cattatttta gcagaccccg gccgcagtta
     2041 cttgcaggca tttttagatg agcttcaagg tttcgggttt cgctacgata tattgattcg
     2101 aaacgtcctc gatggacaca acgaccgagc cggtgatcgc cgtaccaagg aagttttcgt
     2161 aatctcctgc actagtcaaa gctaataact tttcaccgtg ggtgtttaag tcgaatgcgc
     2221 tcgacaactg tgcgcagttc aatattttcc ggttcgtact ttagggcttc ttcgaactcc
     2281 gcgagagctt ccgagaactg acaccgcgaa agaaatatgc ggcccatatt caggtacggt
     2341 gtttggcgtt tgtcatagcg agctgctttt ttggcttttt cgagccaagg ttcagcggcg
     2401 tctagatcgc ctagtttcat gtagtacatg ccaatatcgt tgtaggcgtt gccaaactcg
     2461 caatcgagtt caactgcttt ttcacacaac gcgatggcgt cctgatattt atgttcgaaa
     2521 ctcaaaaccc aagccaaatg ggtgtatgcc tccgccgacg gctgccaccg gagcgctagt
     2581 tcaagtttgt tttttgcggg ttttagaagc ccctgatgca acaagctgac ggcttcctcg
     2641 agtagttttg ctccctgtcg atgcgagagg tttaataagg ttccatctgc cattcgttct
     2701 ccttccatgg agcgcccaag caaatgcggg cgacagctct aatgttagcg tgttttagtt
     2761 ttttggaagc aaaatcttag aacgcgccgc aataagacga tcggctaatt ccgcgaaacc
     2821 gaaactacct tcacgttccg tgatgtaggt tggtgcgtag tccatttttt taagaaacgg
     2881 cgcgacattc gctaccccta cggtaaatgg gagcgcttcg aaaaacggct catcgttggg
     2941 actgtcgccg atataggcaa tttgtgcgag agtttttgca tcaacggccc cgtaaaaaga
     3001 tttcaacacc cgccgtacac aagttagttt gtcatagcga ccgaaccaag tgttgacgtg
     3061 aatgctcgag agtttggcga tcgccccatg acttcgacaa aat
//
LOCUS       DAOMXO010000229         3102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17623_length_3102_cov_3.618969, whole genome shotgun sequence.
ACCESSION   DAOMXO010000229 DAOMXO010000000
VERSION     DAOMXO010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17623_length_3102_cov_3.618969"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLH57_05760"
     CDS             complement(<1..422)
                     /locus_tag="PLH57_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02153.1"
                     /translation="MRHIFVRSFLLVAIATMSIFTIFAAAEHGPVKRFEIKSAHGGTA
                     LIVVSGGYGSKGLTKARFFFETNNPEKIQDLLSEKLKYGATKNELRLALESAKTAPVN
                     ASSSEGTLVEVTSFLPPVFRRTGHFAEQRPYPIPANGKV"
     gene            704..2377
                     /locus_tag="PLH57_05765"
     CDS             704..2377
                     /locus_tag="PLH57_05765"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HRK02154.1"
                     /translation="MNTESTIHPFPGVQVGKLSSKSSTHLRLLNELSVKLQSLLHSDN
                     IYEQIVSAVQNKFHYHSFSIWSVSQDQTAMLRAQSGAYNKFIQPGFTIKGEGILGHVI
                     STRKTYIASDVSQDELFTNLSLPVETKSQISIPVFVDQTLVAVLTVESNQVDAFDEED
                     VATYESLSAQLSVALTNSKLVGEIKSFNKKLQQTVDERTQELRKAHQRILEQQRLLQK
                     ENKVLKNLVTHEKQNEANIIGNSPALNNIISMVDKIAPTHATVLIQGESGTGKELIAR
                     RLHEKSERANAPYVTINCGALQETLLESELFGHEKGSFTGAVVQKMGLVETADGGTLF
                     LDEIGEMSLGIQAKLLRFLQEGEVYRIGGKKPIRVNVRIISATNRDLESEVKNSRFRE
                     DLFYRLNTITLRMPPLRKRKEDIPMLTDFFLNNSRYGGAAHIKHVDPKVYETFQNYDW
                     PGNIRELQNTIERLKILADHNEIRIDDVPFNIRMPRTRVEGASSTSGEALVDMSLDEL
                     EKQHILKVLSYHHGNKTKTAHSLGITIKTLYNKLHRYGLLKEAGGLNPQ"
     gene            2442..2756
                     /locus_tag="PLH57_05770"
     CDS             2442..2756
                     /locus_tag="PLH57_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02155.1"
                     /translation="MAAKAQTQSKSPGKAPKKMPAPPVVVAANLEDDEDLEDAEASAS
                     SGGSGPKVEEATTPLTTKNFRNHPDMENFFRFIHDNDLRLEALQIIDEIIAERAVRKA
                     KK"
     gene            2767..>3102
                     /locus_tag="PLH57_05775"
     CDS             2767..>3102
                     /locus_tag="PLH57_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02156.1"
                     /translation="MTSRVKFAIKAPLYEGSFFLGIRAFRVLFFSTVFALAVSLGSNA
                     KLHAQDEHPKSTPPTAEQIAIECQEVLDGARELQDEVGELAQSLELHVPPRQAMMLAR
                     RAHAIDRADY"
BASE COUNT          904 a          772 c          693 g          733 t
ORIGIN      
        1 actttgccgt ttgccggaat tggatacggt ctttgttcgg caaaatggcc ggttcttcta
       61 aaaacaggag gtaaaaatga ggtgacctcc acaagagtcc cctcactcga gctcgcattt
      121 actggtgcgg ttttcgccga ctcgagagcc aatcgcagct cgtttttggt agcaccatat
      181 tttaattttt cgctgagaag gtcctgaatt ttttctgggt tatttgtttc gaaaaaaaat
      241 cgcgctttgg taagtccttt acttccgtaa ccaccactca ctacgatcaa cgcagtccca
      301 ccgtgcgcgc ttttaatctc gaaacgtttc actggcccat gttctgcagc tgcaaaaatt
      361 gtaaaaatcg acatagttgc aatcgctacg agtaaaaaag accgaacgaa aatatggcgc
      421 actgaagacc tcctacctga cccgaccgag tcattttccc tggaaccctt atgcaacgaa
      481 acgactcatc atgtcaaatt ttagacaagt cattgaaatt atgatgaaaa attccagaca
      541 aaactggacg gaaattaagt attgactcgc agtgtaatct ttatataatg cgcaccggtc
      601 tgtgaaagtc ttacagacca gggcccgcta aaatctacat ggtttcggca ggcaaaaagt
      661 tcgagttttg gggcaacggc acggaattgg agtcgcgaaa aatatgaata ctgaatcaac
      721 cattcacccg tttcccgggg tccaagttgg taagctaagc agcaagtcgt ccactcattt
      781 gcgcttactc aacgaactta gcgttaaact gcagtccctg cttcattccg acaatattta
      841 cgaacagatt gtttcggccg ttcaaaataa atttcattac cattcgttta gcatttggtc
      901 agtaagccaa gaccagaccg ctatgcttcg tgctcaatcc ggtgcctaca ataaattcat
      961 acaaccgggt ttcacaatta agggcgaagg tattttaggt cacgtcattt ccactcgaaa
     1021 aacctacata gcaagcgacg tttctcaaga cgagttattt actaacttgt cgttgcccgt
     1081 agaaacgaaa tcgcaaatat caattccggt tttcgtagat caaacgttag tcgcggtact
     1141 cacggtcgaa agcaatcaag tcgatgcctt cgatgaagaa gatgtcgcga cttacgaaag
     1201 tttgagtgct caactgtcgg ttgcactcac caactctaaa ctcgtgggtg agattaaatc
     1261 atttaacaag aaactgcagc aaacagtcga tgaacgtaca caggaactgc gtaaagctca
     1321 tcagcgtatt ttggagcaac aacgtttgct gcaaaaagaa aataaagtgc ttaaaaacct
     1381 cgtgactcac gaaaaacaaa acgaggctaa cattatcggc aacagcccag cactcaacaa
     1441 tatcattagc atggtcgaca aaatcgctcc aacccacgcc acggtgctta ttcagggcga
     1501 gtcgggaacc ggcaaagaat taatcgctcg acgtttacac gaaaaaagtg aacgtgcgaa
     1561 cgcaccatac gtgaccatca actgtggcgc tcttcaagaa accttgctcg agagcgaatt
     1621 attcgggcac gaaaaaggtt ccttcactgg agcggtggtt cagaaaatgg ggttggtcga
     1681 aacagccgat ggcggcacgc tatttctcga cgaaatcggt gaaatgagcc tggggattca
     1741 agccaaactt cttcgttttc tccaggaagg tgaagtttat cgcatcggcg gcaaaaaacc
     1801 gattcgggta aacgtgcgta tcatcagcgc aaccaatcgc gacctcgaaa gcgaagttaa
     1861 aaacagtcgt tttcgcgagg acttatttta tcgcctcaat accattacgc tgcgcatgcc
     1921 tccgctgcga aaaagaaaag aagatatccc catgttaacg gacttcttct tgaataactc
     1981 tcgctacggt ggtgcggccc atatcaagca tgtggacccc aaggtgtacg agacctttca
     2041 gaactacgac tggccgggca atattcggga gcttcaaaac actatcgagc gcctcaagat
     2101 cctggctgac cacaacgaaa ttcggatcga cgacgtgccc tttaacatta gaatgccgcg
     2161 aacccgagtc gaaggagctt cttcgaccag tggagaagcg ctggtggaca tgtcgctcga
     2221 cgaactggaa aaacaacata ttttaaaggt tttaagttat caccacggaa acaaaaccaa
     2281 gacggcgcat agcttgggca ttaccatcaa gacgctctac aacaagctcc accgctacgg
     2341 ccttttgaaa gaggccgggg gcttaaaccc acaataaacc cctcttgtat ggtgtgaagg
     2401 aattcgatag gttaacgctg aaatcactaa ggagcattga aatggccgca aaagcacaaa
     2461 cccaaagcaa aagccccggc aaagccccca agaaaatgcc ggccccccca gttgtcgtcg
     2521 cagcaaacct tgaagacgat gaagatttag aagatgccga agcttcggca agctcgggcg
     2581 gatcgggtcc aaaagtcgaa gaagccacca cgcctctgac gaccaagaac tttcgcaatc
     2641 accccgacat ggaaaacttc tttcgattta tccatgacaa tgacctgcgc cttgaagcgc
     2701 tgcagattat tgacgaaatc attgcggaac gcgcagttcg caaagctaag aagtaattca
     2761 gtcaatttga cttcgcgcgt taagtttgcc ataaaagccc ctctttacga ggggtctttt
     2821 tttttgggta ttcgggcgtt tcgcgtatta tttttttcta cggtatttgc attggcggtc
     2881 tcgcttggaa gcaacgccaa actgcatgcc caagatgaac atcctaaatc tacccctcca
     2941 actgcggaac agatcgcaat cgagtgccag gaagtcttgg acggcgcccg agaactccaa
     3001 gatgaagttg gggaacttgc acaatcttta gaactgcacg tcccgccacg tcaggccatg
     3061 atgctggctc gccgtgccca cgccatcgat cgcgctgatt ac
//
LOCUS       DAOMXO010000230         3093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17703_length_3093_cov_4.846939, whole genome shotgun sequence.
ACCESSION   DAOMXO010000230 DAOMXO010000000
VERSION     DAOMXO010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17703_length_3093_cov_4.846939"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLH57_05780"
     CDS             <1..610
                     /locus_tag="PLH57_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRK02157.1"
                     /translation="KGYMIKEYFQNYALHNSDVTVDLESGRTEVHYRRAEPWRVTLVD
                     TGEDSMTGGRLLRAKPYLEDAPLFMLTYGDGLGNVDISKLLAFHKELGRAATVTAVQP
                     PGRFGALHFGTDHKVTSFTEKPIGDGGRISGGFFVFSQNIFKRITDDTTILEQHPLKS
                     LAADGELAAFRHDSFWHPMDTLRDKNLLEDLWATGKAPWKLW"
     gene            604..1650
                     /gene="rfbG"
                     /locus_tag="PLH57_05785"
     CDS             604..1650
                     /gene="rfbG"
                     /locus_tag="PLH57_05785"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HRK02158.1"
                     /translation="MVELDVYKNRRVLVTGHTGFKGSWLCLWLTKLGAKVYGYALPEE
                     NGPAAYSALEIRNCLADEALADLRNIEELSTFAEKVSPEIIFHLGAQALVRKSFIQPL
                     ETIQTNILGTAHILEILRKKKVPAVIVTSDKCYENLETGKSYVETDPMGGYDIYSMSK
                     GSCELLTASYRRSFDLNVASARAGNVIGGGDFAEDRILPDCVRSARAKRPVLLRNPDS
                     VRPWQHVLDPISGYLTLGAQLLGPDSIGFREGWNFGPELTSTITVRKLVETFFEYYGP
                     GEIQIDQEKQPHEAKLLHLDINKAKNRLRWAPRWNVQEAVSETSLWYRNFDSGGMMKN
                     YSLDQISRYERSEK"
     gene            1659..2108
                     /locus_tag="PLH57_05790"
     CDS             1659..2108
                     /locus_tag="PLH57_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRK02159.1"
                     /translation="MIDGVVITPKKKIVDDRGAIFHMLRNDEPVFSKFGEIYFSKVHP
                     GVVKAWHIHRSMTLNYFLVTGSVRLALFDDRESSHSRGVFQEIYLDESDSKLVTIPPL
                     VWNGFKGLGSNSSIVANCSTEPHDPEEISRRPFDDIYFKYDWSQKNG"
     gene            2105..>3093
                     /gene="rfbH"
                     /locus_tag="PLH57_05795"
     CDS             2105..>3093
                     /gene="rfbH"
                     /locus_tag="PLH57_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HRK02160.1"
                     /translation="MTSSIRETVIAAARQYFRSEHRSPDFVAGKTFIPAATKVMDEED
                     LVNLVESSLDLWLTAGRFAREFEKELAAFFGMKTSAVLVNSGSSANLCALSSLGAPML
                     ETMGQKPLLPGDEVITAAAAFPTTVNPILQNGWVPVFIDVDSRTLNSLPEAVMAAKSP
                     RTRAVILAHTLGNPFRADLLAEWCKKENLFLIEDCCDALGAQIIDRGNVGTFGHYATA
                     SFYPAHHITMGEGGAVISANAKLRRVAESLRDWGRDCWCEPGQDNTCKKRFCWQLGDL
                     PYGYDHKYTYSNIGYNLKATDMQAAVGLSQLKKLSGFVEKRRANWSALYKGVT"
BASE COUNT          852 a          721 c          748 g          772 t
ORIGIN      
        1 caagggctac atgatcaaag agtattttca gaactacgct cttcacaata gcgacgtaac
       61 agttgaccta gaatccggac gtaccgaggt tcactatcgc cgggcggagc catggagagt
      121 aactctagta gatacagggg aagactctat gaccggagga cggttgctgc gagctaaacc
      181 ttacctagaa gacgcccctc tgttcatgct gacctacggc gacggactgg gtaacgtgga
      241 tatttccaaa ctcctcgcct ttcacaaaga gctcggacgg gcggcaactg tcaccgccgt
      301 tcaaccacca ggccgtttcg gtgcacttca tttcggcact gatcataaag taacaagctt
      361 tacggaaaaa ccaatcggtg acggcggtcg aatcagtggt ggcttttttg tcttcagtca
      421 aaacatcttt aaaaggatta ctgatgacac tacgatattg gagcagcacc ctcttaaatc
      481 gcttgccgcc gacggagaac tcgcggcctt caggcacgat agtttttggc acccgatgga
      541 cacccttcga gataaaaatt tgcttgaaga tctttgggcc accgggaaag ccccctggaa
      601 attatggtag aactcgacgt ctataaaaat cgacgagttc tcgttactgg ccacacgggt
      661 tttaaaggca gttggttgtg tctctggctc accaagctcg gtgccaaagt ttatggctat
      721 gctttgccag aagaaaatgg ccccgccgct tattcggcac tcgaaattcg aaattgtttg
      781 gcagatgagg cactcgcaga tcttcgaaat atcgaagaac tcagtacctt tgctgaaaag
      841 gtaagtcccg aaattatttt ccaccttggt gcgcaggcgc tagttcgaaa atcctttatt
      901 caaccgctcg aaacgattca gaccaacatt ctaggcacgg ctcatatttt agagatactg
      961 cgaaaaaaaa aggtgcccgc tgttatagtt acgagtgaca agtgttacga gaaccttgaa
     1021 acaggaaagt cctacgtcga gaccgatccg atgggcggat acgatattta cagcatgtcg
     1081 aagggttctt gcgaattact gacagcatcc tatcgacgtt cattcgatct aaatgtggca
     1141 agcgctcgag cgggtaacgt gatcggcggt ggcgatttcg cagaagatcg catattaccc
     1201 gactgtgttc gctctgcccg cgcgaaaaga cccgttttat tgagaaaccc agatagcgtg
     1261 cgaccttggc aacacgtatt agacccaatc agcggctacc tcacactcgg ggcccagcta
     1321 ctcgggccgg actccattgg gtttcgagag ggctggaact ttgggccgga actgacttct
     1381 acgatcactg tacgcaaact ggtagaaact ttttttgagt actatggtcc aggcgaaatt
     1441 caaatcgacc aagagaaaca gccacacgaa gccaaactct tgcacctcga cataaataaa
     1501 gccaagaacc gccttcgctg ggcccctcga tggaacgttc aagaagcagt atcagaaacc
     1561 agcctttggt atcggaattt cgattccggt ggaatgatga aaaattatag cttagaccaa
     1621 atttcacgtt acgaacggag cgagaaatga aaataggaat gatagacggc gttgttatta
     1681 ctccgaaaaa gaaaattgta gatgatcgag gagcgatttt tcacatgctt cgcaacgacg
     1741 aacctgtttt ctcgaaattt ggagaaatct attttagcaa ggttcaccct ggagtcgtta
     1801 aagcctggca tattcaccgc tctatgactc tgaattattt tttagtgact ggaagtgttc
     1861 gattggcact atttgatgat agagaaagtt cacattcccg tggcgtcttt caggaaattt
     1921 atctcgacga atccgatagt aagcttgtaa ctataccgcc cttagtgtgg aacggattta
     1981 aaggtcttgg ttctaacagt tccattgttg ctaattgttc gactgaacca cacgatcctg
     2041 aagaaatttc tcgccgacca ttcgatgata tctattttaa gtacgattgg agtcaaaaga
     2101 acggatgacc tcgtcaatac gtgaaacagt aattgcggct gcacggcagt actttcgcag
     2161 cgaacatcgg tcgcctgatt ttgttgctgg aaaaaccttt attcctgccg caacaaaagt
     2221 aatggatgaa gaagacctag ttaatctagt ggaatcttct ctggatctgt ggcttaccgc
     2281 gggacgcttc gctcgcgaat tcgaaaaaga attggcagca tttttcggaa tgaaaacttc
     2341 cgcagtgctt gtcaatagcg ggtccagtgc aaatttgtgt gcactcagta gtctaggggc
     2401 acccatgcta gaaaccatgg gacagaaacc gcttcttcca ggtgacgagg tcataaccgc
     2461 ggctgcggca tttccgacca ctgtaaatcc catacttcaa aacggatggg ttccggtatt
     2521 cattgatgta gactctagaa ctctcaactc tttgccggaa gcagtcatgg cggcaaaatc
     2581 gccgcgaacc agagctgtta ttttagccca cactcttggg aatccttttc gggcagattt
     2641 attggccgag tggtgtaaaa aagaaaatct atttttaatt gaagattgct gcgacgctct
     2701 gggagctcaa atcatagacc gtggtaacgt aggaactttt gggcattacg caaccgctag
     2761 tttctacccg gcccaccaca ttactatggg cgaaggtggc gcagtaatat cagcaaatgc
     2821 caaattgcga cgagtggccg aaagcttgcg tgactggggc agagattgct ggtgcgagcc
     2881 cgggcaggac aacacctgta aaaaaaggtt ttgttggcaa ctcggtgatt tgccctacgg
     2941 ctacgaccat aaatacacct attctaatat tggctataat ttaaaagcca ctgacatgca
     3001 ggccgctgtt ggactctcgc agcttaagaa actttcagga tttgtagaaa aacgcagagc
     3061 taactggagc gcgctataca agggcgtgac cca
//
LOCUS       DAOMXO010000231         3093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17704_length_3093_cov_4.820935, whole genome shotgun sequence.
ACCESSION   DAOMXO010000231 DAOMXO010000000
VERSION     DAOMXO010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17704_length_3093_cov_4.820935"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLH57_05800"
     CDS             <1..276
                     /locus_tag="PLH57_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HRK02161.1"
                     /translation="KFVHTPVGIFELKYFFNSGISAADGGGDVASEAVKEKIRLMLSK
                     EDATKPLSDQRIVELLKAENIDIARRTVAKYRDMLGILSSSKRKKVF"
     gene            340..2028
                     /locus_tag="PLH57_05805"
     CDS             340..2028
                     /locus_tag="PLH57_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02162.1"
                     /translation="MKAASFGLGILLCWVFISTSQAAPSSKAVGIEPRPPRWYSKLSP
                     LKIANALPCQSVVSAGAPTRELLHKLANQKVSLPASRAIDELIQNHLPEVAGIVADFL
                     SVQPAGTVTTEDALQEAFLALSEIVYSEQGPEKKSPFSYRLRMLVLRRLQNLVTQEQE
                     WNELEFVSSDVQLSGSPGEQEQTANLATVRELVKFAVSKDLVTEDNFSEYLDFHANGS
                     TVQEIGSERGVSPQAISMRIKNVSDALASASERLQNTKVNRYRRKVRSTNLKPANSVE
                     IGRKHLIWPDQSSLKFSDLKRSRWLAHIKAPLQLKVFDQFGRVLMVKEPGQSGWSFPE
                     VQVHPDYSIEELALYYLKFRVGLPVASVSKPELNTDQDSKIVWVPISTENILHQRPVF
                     ELKLKTSNTQPAYDNTTGRLHTTWVSEPYAEKLARAPVILTKSISRSGVFVAMNSKDE
                     MRIRAVIRNSEGKILLVRASREGSGWSYPEILVESGNQPEDGLLQAIRKMSGLRENEL
                     REQTKPIDRKTIEDTSVRSSRTLYLRSKNDALSPRNGYEAQWFSLQDVKTLRSE"
     gene            2052..2357
                     /gene="raiA"
                     /locus_tag="PLH57_05810"
     CDS             2052..2357
                     /gene="raiA"
                     /locus_tag="PLH57_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRK02163.1"
                     /translation="MQIHITFKHMDATEALKKHTEEKTAKLEKFANGPFNADWVLVVN
                     AKEHAAELRVHGPNLDYFAHAKTSDMYVSIDEVVSKLETQLRRHKEIVKDHLHRKRD"
     gene            complement(2359..>3093)
                     /locus_tag="PLH57_05815"
     CDS             complement(2359..>3093)
                     /locus_tag="PLH57_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02164.1"
                     /translation="LINDDDSQPGHSHFVYDFKGKLLFDDTVERILKNADYPTNLEMS
                     DAYVDRKTGKISLFFTGGIMIVVDSLGTILHKWRLPEPISAVTVSEDGLYYALTSNLL
                     FALGSGSSDWLWKSRLPSHGDALAVVGDKIVVHANAQNEQWVAAYEKNTGKPEWFYSN
                     NEPALVTPPFQTQRDVAIGFFDMRNNGRIQFFGLNQKGKALWAKNLFAPNGIFSMAAA
                     RPNVVIGYGAPKKGTVTYFKIAESCN"
BASE COUNT          867 a          709 c          734 g          783 t
ORIGIN      
        1 aaattcgttc acactccagt tggaattttc gaactcaaat atttcttcaa cagtggcatt
       61 tcggcagccg atggcggcgg ggatgtggca tccgaggcag ttaaagaaaa gattcgactt
      121 atgctttcta aagaagatgc caccaaaccc ctttcggacc aaagaatagt ggaactgctc
      181 aaggccgaga atatcgacat tgcccgccgt acagtggcta aatatcgaga tatgttaggt
      241 attttgtcct cctccaagcg caagaaagtg ttttaaacca cgatttgatc ccctgtgtta
      301 ttgttgactt tttttgtcta aatagctaaa aactgcggca tgaaagccgc gtcatttgga
      361 cttggaattc ttctttgttg ggtctttatt tcaacttcgc aggcggctcc ttcgagtaaa
      421 gcagtgggta tcgaaccccg cccccctcgc tggtattcca agctttcccc tttgaagatt
      481 gccaacgcct taccctgtca aagtgtagtg agtgctggcg caccaacgcg tgaactttta
      541 cataagctcg caaaccaaaa agtcagtttg cctgcctcgc gtgcgatcga cgaactaatt
      601 caaaatcact tacccgaggt tgctggtatt gttgccgatt tcttgagcgt gcaaccagca
      661 ggtacagtaa ctaccgaaga tgctttacaa gaagcctttc tcgcactcag tgagattgtc
      721 tactcggaac aagggccaga aaaaaaatca ccattttctt accgtttgcg aatgttggtt
      781 ttacgccgcc tccaaaattt agtcactcag gaacaagaat ggaacgaact ggaattcgtg
      841 agttcggacg tgcagctgtc cggatcaccc ggcgagcaag aacaaactgc caacttggct
      901 acagttcgcg agttagttaa atttgccgta tctaaagatt tagtgaccga agataatttt
      961 tcggagtacc tcgattttca cgcgaacggg tcaactgttc aagaaatagg cagcgaacgc
     1021 ggtgtatctc ctcaggccat atcgatgcga attaaaaacg tatcagacgc tctggcatcg
     1081 gccagcgagc gactgcaaaa tacgaaggtt aatcgatatc gcagaaaagt tcgtagcact
     1141 aacttaaagc cggcgaactc agtcgaaatc ggtcgaaaac atttaatctg gcccgaccag
     1201 tcttctttga aattttcaga tttgaaacga agccgatggc ttgcccacat aaaagcaccg
     1261 ctacagctga aagtgtttga tcaattcggg cgcgttttaa tggttaaaga accgggacag
     1321 tcggggtggt catttccgga agttcaggta cacccggatt actcgattga agagttggcg
     1381 ctctattact tgaaatttcg ggtcgggctc ccggtggcat cagtttcaaa gccagaacta
     1441 aacacagatc aagattcgaa aatagtgtgg gtgccaattt ctacagagaa tattttgcac
     1501 cagcgccccg tttttgaact taagcttaag accagcaaca ctcaaccggc ttacgataac
     1561 actaccggtc gtctacatac tacttgggtt agtgagccat acgccgaaaa attagcccga
     1621 gctccggtaa tacttacaaa atcaatttct cgatcgggtg ttttcgtcgc catgaattct
     1681 aaagacgaaa tgcgcattcg agcagttatt cgtaactcag aaggtaagat tcttttggtt
     1741 agggcgtctc gcgagggttc ggggtggagc tacccggaaa ttttggtgga gtccgggaac
     1801 cagccggaag acggtctttt gcaggcgatt cgaaaaatga gcgggttaag ggaaaacgaa
     1861 cttcgcgagc aaaccaaacc gatcgatcga aaaacaattg aagacacatc ggttcgaagc
     1921 tcgagaaccc tgtatctgcg atcgaaaaac gacgctctaa gtccgcgaaa tggttacgaa
     1981 gcgcagtggt tttcgttgca agacgtaaag accttgcgat ccgagtagtt ttgcaggaag
     2041 atcggccacc tatgcagatt cacattactt tcaaacacat ggacgccacg gaggcgctca
     2101 agaagcacac ggaagagaaa actgccaaac tcgaaaaatt cgctaacggg ccgttcaatg
     2161 ccgattgggt gttggtcgtg aatgctaagg aacatgcggc tgaattgcga gtgcacgggc
     2221 caaatttgga ttactttgcg cacgctaaaa ctagcgacat gtatgtttca attgacgaag
     2281 tagtttcgaa actcgaaacg cagctgcgtc gccataaaga aatcgtcaaa gaccacttac
     2341 accgaaagcg cgactaggtt aattgcagct ttcggcaatt ttgaaataag ttacggttcc
     2401 ttttttagga gcaccgtatc cgatcacaac gttagggcgc gcggctgcca tagagaaaat
     2461 gccgttcggc gcaaacaagt tctttgccca taatgctttg cctttttgat ttaggccgaa
     2521 aaattgaatt cgaccattgt tccgcatatc gaaaaacccg atcgccacat cacgttgggt
     2581 ttgaaaaggt ggagtcacta acgctggttc attgttggaa taaaaccatt cgggttttcc
     2641 ggtgtttttt tcgtaagcgg caacccattg ttcgttttgg gcattcgcgt ggactacgat
     2701 tttatcaccc acaaccgcaa gagcgtcacc gtgagaagga agtcgtgatt tccaaagcca
     2761 atccgaactt ccagagccta gggcgaaaag taaattagaa gtaagtgcgt aatataagcc
     2821 gtcctcgcta acggtaacgg cggagatagg ttctggcagt cgccacttgt gcaaaatagt
     2881 tccgaggcta tcaactacga tcatgattcc accggtaaaa aagagcgaga tcttgcccgt
     2941 cttccggtca acgtaggcgt cgctcatttc taaattagtc ggatagtcag cattctttaa
     3001 aatgcgttcg acagtatcgt cgaacaataa ctttccttta aagtcgtaaa cgaaatgaga
     3061 atgacccggc tgagagtcgt catcgttgat tag
//
LOCUS       DAOMXO010000232         3087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17754_length_3087_cov_5.725594, whole genome shotgun sequence.
ACCESSION   DAOMXO010000232 DAOMXO010000000
VERSION     DAOMXO010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17754_length_3087_cov_5.725594"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3087
                     /locus_tag="PLH57_05820"
     CDS             <1..>3087
                     /locus_tag="PLH57_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02165.1"
                     /translation="ITSQFQTQNILGEKIEFTGAPAKAVVSYSMRLQDPDSGDYLASG
                     VYPYTSHWEVHLKRIVVRTRKITDTRWAQNLIIAKASFRKIIEQFFPSDLNDFIYVQN
                     ERESAAGSGWKIAGAQKIANPGKERLMIEEADGSIAGYAINNTITTAFNGSSHNAEFS
                     DGAVNFLNYPDIYLNLTSRRVGRIDLSSNNPIPQVLATLPNLTADMRSYDAEAYNGNW
                     NLHQCRYHYYSFSMGRMPSSLIMHPDGRLFALDRSTSELYHLTNGQFNYFSGPLRNPT
                     TWIGTHSHNIGEYNYYAQNCPTCKDANAAGFTTKGWSSIDAALNDCGEVGAQCSYLGR
                     NDESGFPRCQALQPRGHVSTGQYPNFAFGGDGGLASSANFNRPQGMTVGTGNSIVIAD
                     TGNNRVRRIDLSTNVVTTLAGNGQTFDTGDGGLAVDASLYHPRGVAYDTVGNLYIATE
                     NGFIRKVDPTGRISTFAGKTLADGGTIGDNLDAADVFLNKPYGLVVDNDNGFLYVAEE
                     GAHLVRRINLNTREVATVAGTGAAGFSGDSGPAITSQLNRPTHLGLDENGNLLIADAG
                     NNRIRKVVFQNAATGVLAFAPIAKDHSKLTRQADGTWLREYRSGVKVYFDVNGRQTAA
                     VDRVGRTTTMTYDGIGNLTSITDPTGRAVNYNYSGGKLSSIVDPAGRATSFHYSGSYL
                     THVTYPDGAQKQFGYNAQGLLATETNERGHPTSYVYNEWSRLQQVVRADNSAVTVNDS
                     GSGTAGNNYVGGTATGQLKAYNENSNYDGIKDARDVETKFVKDSNGYVTTIVDGAGRT
                     TQVTRDEKGKPLKITRPDGSEVTFVYDPATDDLLAQTDSALGVTTSQTFDAFGNLLTQ
                     TNGRGFTSQNTYNPNNGLLVSKTDPLGHGASYTYNNLGMPLSVTNALNKSTVYTYDAF
                     GNVATVRDQLGHTTTYTRDAAGNVISVRNAKGQVTLYEYDLWNRLVAVTTPKNERTEY
                     SYLASGELAQIKDPLNNITTFEYDNLGRLTKKTDPLGYAITRSYDQNGNVVGEIDP"
BASE COUNT          831 a          813 c          714 g          729 t
ORIGIN      
        1 cattacttcg caatttcaaa cccaaaatat tttaggcgaa aaaattgaat tcactggtgc
       61 gccggctaaa gcggttgtgt cttattcgat gcggctgcaa gatcctgaca gtggcgacta
      121 cttggcctcc ggcgtttacc cctacacctc gcactgggaa gtgcacttaa agcgcattgt
      181 cgttcgcacc cgaaaaatca ccgacacccg ttgggcgcaa aatcttatca ttgccaaagc
      241 cagttttaga aagattattg agcagttttt ccccagtgat ttgaatgatt tcatctacgt
      301 gcaaaacgaa agagaatctg cggctggtag tggctggaaa atcgcaggcg cccaaaaaat
      361 cgcaaatcca ggcaaagagc gtttaatgat cgaagaagcg gatgggtcaa ttgctggcta
      421 cgccatcaac aacaccatca cgactgcctt taacggcagt tctcataacg ccgaattttc
      481 ggatggcgcc gtaaattttc taaattatcc cgacatttat cttaacctca cttcgcgcag
      541 agtgggccgc attgatcttt catcaaacaa tcccattccc caagttctag ccaccctgcc
      601 aaacttaacc gctgacatgc gctcttacga cgctgaagcc tataacggaa actggaacct
      661 gcatcagtgc cgttatcact attacagttt ttcaatgggc cgtatgccaa gctcgctcat
      721 aatgcatccc gacggcaggc tctttgcact cgatcgatcg acttccgaac tttatcacct
      781 gactaatggc caatttaact atttctcggg tccactgaga aacccaacca cctggatcgg
      841 cacccacagt cacaacatag gcgaatacaa ctactacgcc cagaactgcc ctacctgtaa
      901 agacgccaac gcggcgggct ttacgaccaa aggctggtcc tcaattgatg cagcccttaa
      961 cgattgcggt gaagtgggag ctcaatgcag ctacttaggt cgtaacgatg aaagcggttt
     1021 tccacgctgc caagctttgc agccgcgcgg ccatgttagc accggtcaat atccaaattt
     1081 cgcgtttggg ggcgacggtg gcttagcgag tagtgctaat ttcaatcgcc cgcaaggcat
     1141 gacggtaggc acaggtaatt ccattgtcat tgccgacacc ggtaacaacc gagttcgaag
     1201 aattgattta agcaccaacg tcgtcaccac cctggccgga aatggccaaa cttttgatac
     1261 cggcgatggt ggtttagcgg tcgacgcttc actttatcat ccccgcggtg ttgcgtacga
     1321 cacagttggt aatctgtata tcgcaactga aaatggtttt attcgcaagg tcgatccgac
     1381 tgggcgtatt tctacatttg cgggtaagac tttagccgac ggcgggacga ttggcgacaa
     1441 tctcgatgcc gccgacgtgt ttttgaataa accgtatggc ctggtggtgg ataacgacaa
     1501 cggctttctt tatgttgccg aagaaggcgc tcacttagtt cgtagaatta atttgaacac
     1561 ccgtgaagtc gcaacggttg ctggcaccgg cgcagccgga ttctcgggtg atagcggccc
     1621 cgcgattact tcgcagttaa acagacccac tcatttgggc ttagatgaaa acggcaatct
     1681 tttaattgcc gacgccggca acaaccgcat tcgcaaagta gtgtttcaaa atgcagcgac
     1741 tggagttttg gctttcgctc cgattgccaa agaccattca aagctcactc gtcaagctga
     1801 tggcacttgg ctcagagagt atcgaagcgg cgtaaaagtt tattttgatg tgaacggcag
     1861 gcagactgca gcagtcgatc gggtcggccg cacgaccacc atgacttacg acggcatcgg
     1921 taatttaact tcaatcaccg atcctactgg tagagcggta aattacaatt attctggtgg
     1981 aaaactctct tccatcgttg atccagcagg aagagccact tcgtttcatt actcgggttc
     2041 gtatttaact cacgtcactt accccgatgg cgcgcaaaaa cagtttggtt ataatgcgca
     2101 agggttgctg gcaactgaga ccaatgaacg cggtcatcct actagctacg tttacaatga
     2161 atggagtcgc ttacaacaag tagttcgtgc cgataattct gccgttaccg taaacgattc
     2221 aggctcgggc accgctggta acaactatgt cggcggcacc gcaaccggcc agcttaaagc
     2281 ctataacgaa aactcaaact acgacggcat taaagatgcg cgtgacgttg aaacgaagtt
     2341 cgttaaagat tctaacggtt atgtaacgac catcgtggat ggcgcaggcc gcactacgca
     2401 ggttactcgc gacgaaaaag gtaagccact taaaatcacc agacccgatg gcagtgaagt
     2461 tacgttcgtt tacgatccgg ctaccgatga tctattagcc caaaccgact cggcattagg
     2521 cgtcaccacg tcacagactt tcgatgcctt tggcaattta ctcacccaaa ccaacgggcg
     2581 aggttttacg agccagaata cgtacaaccc aaacaatggt ttactcgtca gtaaaaccga
     2641 tccgctgggg catggtgcaa gttacactta taacaatctg ggtatgccgc tctcagtaac
     2701 taacgcattg aataaatcaa cagtctacac ctacgatgcc tttggcaacg ttgctacagt
     2761 gcgtgatcag ctcggtcaca ccaccaccta tacgcgagac gctgctggca acgtaatctc
     2821 ggttcgcaac gccaaggggc aagtcacgct ttatgaatac gatttgtgga acaggcttgt
     2881 cgctgtcacc actcctaaaa acgaacgcac cgaatacagc tatttagcaa gtggcgaact
     2941 cgcccagatc aaagacccgt taaacaacat caccaccttc gaatacgaca acctcgggcg
     3001 tttaaccaag aaaaccgatc cgctgggtta cgctatcact cgaagctacg accaaaacgg
     3061 aaacgtcgtt ggtgaaatcg acccgaa
//
LOCUS       DAOMXO010000233         3084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17784_length_3084_cov_5.468141, whole genome shotgun sequence.
ACCESSION   DAOMXO010000233 DAOMXO010000000
VERSION     DAOMXO010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17784_length_3084_cov_5.468141"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLH57_05825"
     CDS             <1..668
                     /locus_tag="PLH57_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02166.1"
                     /translation="LWTLSQRLIEERLIPEALKEIETYARTIDEAEKKGDIEIYRGAR
                     GAVVAANAEGLRRTNERVIAALFRLAIIELGQFRDFDKTAKKGRQPIRNFFRHRGQAS
                     FDTILAVGGVVSFLPPEFQMPFLGVTHLASEIFHGYMRRLERKEKPSAVYMYIDYLKA
                     LERIAERSSREVAKVMHEILDQNVWTLKSSNYFNQNAVKALCGDVLSAGRLLPATAGT
                     GPR"
     gene            730..2220
                     /gene="gltX"
                     /locus_tag="PLH57_05830"
     CDS             730..2220
                     /gene="gltX"
                     /locus_tag="PLH57_05830"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRK02167.1"
                     /translation="MGVKDFVGDFSAISNEVRVRFAPSPTGYLHVGGARTVLFNWLYA
                     KHTKGKFILRIEDTDQVRSTREAEEMQIEDIKVLGLTYDEGPDVGGPHAPYRQSERLA
                     IYGKYAKELMDTERAYHCFCSEELLTQKREAAMKLGLVPQYDGTCRKIPKDEAAKRLA
                     NGEKATVRFKAPVRTYKLNDAVRGEVVFPENMVGDFVLLRNDGMPVYNFCCVIDDHLM
                     KITHVLRAEEHLSNTVRQMMLYETFGWPLPIMAHCSLILGHDRQKLSKRHGATSVHQY
                     VEEGFLPNALKNFLLLLGWSSPENKEILTEAEMIEQFSLERLNKAPSVFDPVKLRWMN
                     GEYIRHGNLKEMVERAFPYLEKAGLPVKNVSQDWLEKAFDTIRGHLETVADAPKFMRF
                     YFDETFELENEANAVKAEATFAAVAGALKEGLAEHVSAAGDAMSADDFTKLQDRVKTA
                     SGAKGKGLFMPMRVCLTGQVHGPELKLVVPLLGAKRALKRVEQFIR"
     gene            2227..>3084
                     /gene="cysS"
                     /locus_tag="PLH57_05835"
     CDS             2227..>3084
                     /gene="cysS"
                     /locus_tag="PLH57_05835"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRK02168.1"
                     /translation="MADAKKVFRLTNTQSGKREEFRPQDGRKVTFYSCGPTVYGPLHI
                     GNARQLIFADWVFRWLKHIGYDVNFVRNYTDVDDKIIDRAAKEGVSSLQIAEQYIEYC
                     ETDIRALKLLPPTKTVRVTDTIPEIIAMIEQILERGHAYVVDGEVLFSIDSFPNYGKL
                     SGKKTEDLLAGARVEVRSMKRNPLDFSLWKPRKKENTAEPFWPSPWGEGRPGWHIECS
                     AMASRWLGVTIDLHHGGQDLVFPHHENEIAQSEAASGKLFCRHWIHHAFLTSGNEKMS
                     KSLGNIFSIR"
BASE COUNT          825 a          697 c          822 g          740 t
ORIGIN      
        1 agttgtggac tttgtctcag cggctaatcg aagaacggct tattcctgag gctctgaagg
       61 aaattgaaac ttacgcgcgg actatagatg aggccgaaaa gaagggcgat attgagatct
      121 accgaggcgc ccgtggcgcc gtcgtagcgg caaacgcgga aggtctccgg cgtaccaacg
      181 aacgagttat tgccgcgtta tttaggttgg ccatcattga gctcggacaa tttagagact
      241 tcgataaaac tgcgaaaaaa ggccgacaac caattcgaaa tttttttagg caccgtgggc
      301 aagcgtcctt tgacaccatt ctggcggtgg gcggggttgt ttcgtttttg cccccggaat
      361 ttcagatgcc atttcttggg gtgacccatt tggcgagcga gatttttcac gggtacatgc
      421 gacgtctaga acgtaaagaa aaaccatctg ccgtttacat gtatatagac tacctgaaag
      481 cgctggaacg aatcgcggag cggtctagcc gagaggtcgc aaaagtcatg cacgagattt
      541 tggaccagaa tgtttggact ctcaaaagtt ccaattattt taatcagaat gcggtgaaag
      601 cattgtgtgg tgacgtgctc agtgcgggcc gtttgttacc ggcaaccgcg ggcaccggac
      661 ctcgttagac cgaaccttgt acgaaaagca atcctatgcc aaagtggccc cagaatagga
      721 aaactactca tgggcgttaa agatttcgtt ggtgattttt cagcaatttc aaatgaagtg
      781 cgggtgcggt ttgcgccaag tccgacaggg tatttgcacg ttggtggggc gcgcacggtg
      841 ttgtttaact ggctttatgc caaacacacc aaaggcaagt ttatcttgcg catcgaggac
      901 accgatcagg tgcggtctac tcgcgaagcc gaagaaatgc agatcgaaga cattaaggtt
      961 ctgggcctca cttatgacga aggcccagat gtcggtgggc cacacgcacc gtaccgccag
     1021 tccgagcgcc tggcgatcta tggcaaatac gccaaggaac tcatggacac tgagcgcgcg
     1081 taccattgtt tttgcagcga agaattactg actcaaaaac gcgaagctgc catgaaacta
     1141 ggtctcgtac cgcagtacga tggtacctgt cgaaaaattc ctaaagacga agcagcaaag
     1201 cgtctagcca atggcgaaaa agccactgtg cgttttaaag caccggtacg aacctacaaa
     1261 ctaaacgatg ccgttcgtgg tgaggtggtg tttccggaaa acatggttgg tgatttcgta
     1321 cttctacgaa acgacggcat gccagtttat aatttttgct gcgtgatcga cgatcattta
     1381 atgaaaatca cgcacgtatt gcgcgccgaa gaacatttgt cgaacacagt tcgtcaaatg
     1441 atgctctacg aaacatttgg ctggcccctt cccataatgg cgcactgttc gttgatcctt
     1501 ggtcacgatc gacaaaaact ttcgaaacgc catggtgcaa ctagtgtgca ccaatacgtc
     1561 gaagaaggtt ttttgccgaa tgccttgaaa aattttctgc tgcttctcgg ttggtcgtcg
     1621 cccgaaaata aagagattct caccgaagcc gaaatgattg aacagttttc gctcgaacgg
     1681 ttaaataaag ctccgagcgt gtttgatcct gttaagttgc gttggatgaa tggtgaatac
     1741 attcgtcatg gcaatctaaa agaaatggtg gaacgggcgt ttccatatct tgaaaaagcc
     1801 ggtctccctg tgaaaaatgt ttcccaagat tggctcgaaa aagcgttcga tacgattcgt
     1861 ggtcacttag aaaccgtggc cgacgcgcct aagtttatgc ggttttattt cgatgaaacg
     1921 ttcgaactcg aaaacgaagc gaatgcggta aaagccgagg ctaccttcgc agctgtagcg
     1981 ggggctctca aggaaggtct tgcggagcac gtcagtgccg ctggagatgc gatgagcgcc
     2041 gacgacttca ccaaactgca agaccgagta aaaacggcca gtggagctaa gggcaaaggt
     2101 ttgttcatgc cgatgcgggt ttgtttgacc ggtcaagtgc atgggcctga gttaaaatta
     2161 gtggtaccgc ttcttggtgc taaacgcgcg ctcaaacgcg tggaacaatt cattcggtga
     2221 acgttcatgg ccgatgccaa aaaagtcttt cgactgacga atacccagtc cggtaaacga
     2281 gaagaatttc ggccacaaga tggccgaaag gtcacctttt atagttgtgg accgacggta
     2341 tacggaccgc tgcacattgg aaacgcgcgg cagttgatat ttgccgactg ggtgtttcgc
     2401 tggctcaaac acattggcta tgatgtgaat ttcgtgcgca actacactga cgtcgacgac
     2461 aagatcattg atcgagctgc gaaagaaggt gtgtcttcgt tacaaatcgc agaacagtat
     2521 atcgaatatt gtgagaccga cattcgagca ctgaagctct taccgccaac taaaacagtg
     2581 cgggtgacag atacaattcc agaaatcatc gcaatgattg agcagattct tgaacggggc
     2641 catgcctacg tagtcgatgg tgaagttcta ttctcgatcg attcgtttcc gaactacggt
     2701 aaactttcag gaaagaaaac tgaggatctg ctggcagggg cacgagtcga agtgcgttcg
     2761 atgaagcgca atccgcttga tttcagtctt tggaaaccgc gaaaaaaaga aaacacagcc
     2821 gaaccttttt ggccgagtcc ctggggcgaa ggtcgcccgg gctggcacat tgaatgttcc
     2881 gcgatggcat cccggtggtt gggcgtgacc atagatttgc atcacggcgg acaagacttg
     2941 gtgtttccgc accacgaaaa cgagatcgcc caaagtgaag cggcaagcgg aaaacttttt
     3001 tgccgccact ggattcacca tgcgtttttg acttcaggta atgaaaagat gagtaagagt
     3061 ttgggaaata ttttttccat acgc
//
LOCUS       DAOMXO010000234         3076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17866_length_3076_cov_4.634889, whole genome shotgun sequence.
ACCESSION   DAOMXO010000234 DAOMXO010000000
VERSION     DAOMXO010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17866_length_3076_cov_4.634889"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..734)
                     /locus_tag="PLH57_05840"
     CDS             complement(129..734)
                     /locus_tag="PLH57_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRK02169.1"
                     /translation="MNTAKTAKFIVPVFFGLIITWVIFTNNSLVQKEETVNQTWAQVE
                     NVYQRRADLVPNLVNTVKGYAKHEKETLEGVIQARNLALSASGQAKAAPGSATSEDKV
                     KTFAAAQDTLGAALSRLMVVVERYPELKANENFRDLQSQLEGSENRIAVERRRFNEAV
                     QTYNSTVRSFPTSVVAGFRGFEKKAYFEAAQGSDVAPKVDF"
     gene            complement(746..2098)
                     /gene="trmFO"
                     /locus_tag="PLH57_05845"
     CDS             complement(746..2098)
                     /gene="trmFO"
                     /locus_tag="PLH57_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRK02170.1"
                     /translation="MSGKTINIIGGGLAGSEAAYQLAIRGFQVRLFEMRPTHSTPAHK
                     TGGLAELVCSNTFKSLAAHSAHGIFKKELAQLGSLLMKIAPLAQVPAGEALAVDREVF
                     SNEIERALLETGRVERCSALVENIDSLPLADRTIIATGPLTQGPMADYIRSLSNGHGL
                     YFYDAIAPVIDATSIDTKIAFQASRYGKGTDDYWNCPMSREEYDRFYDALIASEKMAF
                     HDFEAAQYFQGCQPIEAIAETGRDSLRFGPMKPVGLEDPRTQKRPHAVVQLRIDNLAK
                     SAYNMVGFQTKMKYGEQIRVFRLIPGLENAEFLRLGSMHRNTYICTPELLKPDFSLNT
                     HPNIHFAGQITGVEGYTESSAIGLLCALAIDCHRKEIPWTLPPKNTATGSLCRYLFES
                     PAKDFQPVNVHFGLIDTSLFNMPGKKIGKNETRTMIGEQCLTNLANWKKSLPDPLNPV
                     "
     gene            2175..>3076
                     /locus_tag="PLH57_05850"
     CDS             2175..>3076
                     /locus_tag="PLH57_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRK02171.1"
                     /translation="MPNVWTPHLVGFKAYLVRKYRSHHTSRNYLSDVHLWIGAFVRDG
                     LVNLRPETSEIRTAMMQFQGLSNRSMQRKLAALSAFFEYLISIGRIENNPCDPLGKPK
                     VERTIPHILAEEHAQTLFRIDPDHEPELTFFRDFIILELLYCTGIRAQELADLTWDRV
                     SLERSEISVIGKGNKFRTIPLVSELVPLLRTYRELQGNYLKSVESENAKFVLFHIKAR
                     GNLRTLIPGLSTRHIQHVVKHFSAAAGLSNKISPHTLRHSFATHLLSAGADLRSIQKL
                     LGHESLSTTQKYTHLSIEKLCEDY"
BASE COUNT          783 a          734 c          739 g          820 t
ORIGIN      
        1 gacaccaaca aaatcgttca tgcgaaaccc tgttggctcg ggtagcgaca acgcgccgct
       61 agcgccagca ggggcgatca agagaaaaat aaaaacagtt agaaaaaaag atcgaattaa
      121 attttccact aaaaatctac tttgggagct acgtcagatc cctgggccgc ttcgaagtag
      181 gccttttttt cgaaacctct aaaaccagct acaacggaag ttggaaagga tcgaacagta
      241 gaattgtaag tctgaacggc ttcgttgaaa cgccgtcgtt ctaccgcgat tcgattttca
      301 gatccttcga gttgagattg taaatcgcga aaattttcat tggctttgag ctcgggataa
      361 cgttcgacca caaccattaa cctcgaaaga gctgccccca atgtgtcttg ggccgctgca
      421 aaggttttaa ctttgtcttc gctagttgcg gaacccggtg ctgctttggc ttggcccgaa
      481 gcagaaagtg ccaaattgcg agcctgaata acgccttcga gagtttcttt ttcgtgtttc
      541 gcgtaaccct taacggtatt gactaagttc gggacgagat ccgcacgtct ttggtatacg
      601 ttctcgacct gagcccaggt ctggttcact gtttcttctt tttgaaccaa agaattatta
      661 gtaaaaataa cccaggttat aataagtcca aaaaataccg gaacaataaa tttcgcagtt
      721 tttgcagtat tcataatgag caaacttata ccgggtttag gggatctggt aacgattttt
      781 tccagttcgc gagattggtt aagcactgct ccccgatcat agttcgggtt tcattttttc
      841 cgattttttt cccgggcata ttaaaaagcg aagtgtcgat caggccaaag tgcacattaa
      901 caggctggaa gtccttggcg ggactttcaa acaaatatct acacagactt ccggtggcgg
      961 tatttttagg aggcaaggtc cacggtattt ctttgcggtg gcagtctatt gccagggcgc
     1021 acaacaaacc gatagcgctg gattcggtgt aaccctctac gcccgtgatt tgccccgcga
     1081 aatggatatt tggatgagtg tttaacgaaa aatcaggttt cagcaattcg ggagtgcaaa
     1141 tgtaagtatt tcggtgcatc gaacctaggc gtagaaactc ggcgttttcg agtcccggaa
     1201 ttaatctaaa tacccgaatt tgttccccat atttcatttt agtttgaaat cccaccatgt
     1261 tgtatgcgct tttcgccagg ttatcgatac gaagttgaac cacagcgtgg ggacgtttct
     1321 gagttctggg gtcctcgagt cccacgggtt tcatgggacc aaagcggagc gagtcgcggc
     1381 cggtttcggc aatcgcctcg ataggttggc aaccttgaaa atattgagca gcttcaaaat
     1441 catgaaacgc cattttctcc gaagcgatta gcgcgtcgta gaatcggtcg tattcttctc
     1501 ggctcatggg gcaattccaa tagtcgtccg tacccttgcc gtagcgtgac gcctgaaagg
     1561 cgatcttagt atcaatactt gttgcatcga taaccggggc gatagcgtca taaaagtaga
     1621 gtccatggcc attcgataag ctacgaatgt aatcggccat tggaccctgg gttagcgggc
     1681 cggttgcgat gatagtgcga tcggccaaag gcaatgagtc gatattttct accaatgctg
     1741 agcatcgttc tacccgacca gtttccagaa gcgctctttc aatttcgttc gaaaatactt
     1801 cgcgatcgac ggctagcgct tctcctgccg gaacctgggc caggggcgct attttcatga
     1861 gaagactgcc aagttgagcc aactcttttt taaaaattcc atgagccgag tgggctgcca
     1921 gcgatttgaa tgtattgctg catactaatt cggcgagccc cccggttttg tgagcggggg
     1981 tcgaatgagt gggtcgcatt tcgaatagtc gaacctggaa ccctcgaatg gccaactggt
     2041 atgcggcttc ggaacccgca agtcctccgc caattatgtt gatagtcttt cccgacatgg
     2101 cgcatcatat accgggaggg cggagcaata gcgaggtatt tttagagact gctctggcgt
     2161 acaatcggtg gtgcatgcct aatgtttgga ctccgcacct ggtgggattt aaggcctatt
     2221 tagtgcgaaa atatcgatcg caccacactt cgcgaaacta cctgtccgac gttcatttgt
     2281 ggatcggcgc ttttgtacgc gatggtctcg tgaaccttag gcctgagact tcggaaatac
     2341 gcacggcaat gatgcagttt cagggactta gtaaccgctc tatgcaacgt aaactcgcgg
     2401 ctctcagcgc tttttttgaa tacctcatct cgataggccg catagaaaat aacccttgcg
     2461 atcccctggg caaaccaaag gtagaacgaa cgattccaca tattttggct gaagagcacg
     2521 ctcaaacgct ctttcgaatc gatcccgatc acgagccaga gctaacgttt tttcgtgatt
     2581 ttataattct agagctgctc tactgtaccg gtattcgtgc ccaggagctt gcagatctta
     2641 cctgggaccg cgtctcactt gaacgaagcg aaatttcggt gatcgggaag ggaaacaagt
     2701 ttcgaaccat tccgttggta tccgaactag tgcctctcct tagaacgtat cgagagctgc
     2761 aggggaatta cctcaaatca gtggaaagcg agaatgccaa gttcgtacta tttcacatta
     2821 aggctcgcgg taatcttcgc acactgatcc ccgggctctc tactcgacat atccaacatg
     2881 tggtaaaaca cttttccgcc gcagcgggac tctcaaataa gatatcgccg catactttgc
     2941 gtcacagctt tgcgactcat ttgttgtcag cgggagcaga tctccgcagt attcaaaaat
     3001 tgctaggcca cgaatctctt tctaccaccc aaaaatatac ccacctatcg atagaaaaac
     3061 tttgcgaaga ttatga
//
LOCUS       DAOMXO010000235         3072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17899_length_3072_cov_4.220749, whole genome shotgun sequence.
ACCESSION   DAOMXO010000235 DAOMXO010000000
VERSION     DAOMXO010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17899_length_3072_cov_4.220749"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PLH57_05855"
     CDS             <1..568
                     /locus_tag="PLH57_05855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRK02172.1"
                     /translation="LLTNNVFFIGRLAGVGVVSASQAIAAGLSGPNLRASGVKLDKRK
                     QEDYEIYRKIDFSTPLGATSADSFRGAVQGDAFNRYFVRIQEIEQSAVIIRQVLANVP
                     EGKYALTLPRVFKVPAGEIYVSVESPRGTQGIFIRSTGEKYPDRLRLRTPSFPVLNIL
                     PAVLESSTLEDMGPLVASFDYLPSEVDR"
     gene            565..1668
                     /locus_tag="PLH57_05860"
     CDS             565..1668
                     /locus_tag="PLH57_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRK02173.1"
                     /translation="MSTILLSEISPFSAFLMSFTDGSTLSTQIFFLIFGFLLFLASFL
                     VPAFFAINYLDRKICGDIQMRIGPNLAGPHGIFQGVADFIKSFEKIRTESQATSGGFF
                     FVTISMAIAFLVFAAINVPVSSQWLYSNVDLSALFVVVALVVSRLFLFWSAYKTANAW
                     STVSAFNMLSVIGYFLVPLTLALVSPALLVGSLDLQNIALAQGGMPWEWMVFNGPGSF
                     FSFASFAVALFIWHGRAPSNLFFESTSVKSEVISEFEGRRYFVLKQIEVFSLFLAAAL
                     IVTVYLGAWRAPFSLESFGRAANIVEACIFFTKTMFVTFVMIWFRWSMPSARLKQISN
                     LSWYLLLPAGFAGVAITLLWLAVSRGEGVRWWL"
     gene            1659..2237
                     /locus_tag="PLH57_05865"
     CDS             1659..2237
                     /locus_tag="PLH57_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRK02174.1"
                     /translation="MALAQGRVFFGVRKNLATAYRVLRVTFPYLFGYRSEYRENTEQY
                     PDRVSARMPEDLPAKVRGILKNDIEKCSGCRYCADVCPVNCIEIETEVGPDRSLSWVS
                     VFDIDHSKCIFCGMCVESCPTGSLIHTKEYEASTEKLDSLVMGFGRGWVTQNMKDAWN
                     REQQAKEAKAEEAAVNELSPISAEIKRRLEGS"
     gene            2234..2764
                     /locus_tag="PLH57_05870"
     CDS             2234..2764
                     /locus_tag="PLH57_05870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRK02175.1"
                     /translation="MTLLGLLLYLLFATTLFCAVYLLFSKNLYRISFALFVLNVSLSG
                     IMLYLGYRAISLIFFSWSAIATVLFLSFSSALVGDLSNGFQKSEGTKKFANYIGLFVG
                     GGFGFGFLWAIKRQGLALDRSSFDPGIPMEALGKQLLGEHSIVLHLIGFLILIVVIGA
                     STLRRRIDGQRSSNES"
     gene            2754..3065
                     /locus_tag="PLH57_05875"
     CDS             2754..3065
                     /locus_tag="PLH57_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02176.1"
                     /translation="MSPNVNDILVLAAILLGLGIGGALFRTNLVVLFAALQTAAAGAV
                     LAICAIGTASETARNTAQIFALIVTVSMSMSSVLGYIIFFSRFRAVKRSNINDAVELR
                     E"
BASE COUNT          711 a          605 c          781 g          975 t
ORIGIN      
        1 gctgctcacg aataatgtat ttttcatcgg ccggctcgcg ggcgtcggcg tcgtttctgc
       61 gagtcaggct attgccgcag gacttagcgg accaaacttg agagcgtcag gggtaaagct
      121 cgataaacgc aaacaagagg actacgaaat ttatcgaaaa atagattttt ctactccctt
      181 aggcgctacc tcggccgact cttttcgggg tgccgttcaa ggcgacgcgt ttaatcgcta
      241 tttcgtaaga attcaggaaa ttgaacagtc cgccgtgatc ataagacagg tgctagcgaa
      301 tgttcccgaa ggcaaatatg cactgaccct tccaagggta tttaaagttc cggcgggaga
      361 gatttatgtt tcggtcgaaa gtccacgcgg aacgcagggt atttttataa gaagcacggg
      421 cgaaaaatat ccggatcgct taagactccg aacaccatca tttccggtac taaatatatt
      481 gccagcagtt ttggagtctt cgaccttaga agacatgggc cctttggtgg cctcgtttga
      541 ttatttgccg tcggaggtgg atcgatgagc acaattcttt tgtcggaaat cagtccattt
      601 tcggcatttt tgatgtcctt cacagacgga tcgacactgt cgacgcaaat attctttttg
      661 atttttggat ttctcctgtt tcttgccagt tttttagtcc ccgcattttt cgcgataaat
      721 tatctagatc ggaaaatttg cggggatatt caaatgagaa taggaccgaa cctcgcgggc
      781 cctcatggaa tattccaggg agttgccgat tttataaagt cgtttgaaaa aattcgcacc
      841 gaatctcagg cgacgagtgg cggctttttt ttcgtcacca tatctatggc aatagctttt
      901 ctggtttttg ccgccatcaa tgtgcccgtc tcttctcagt ggttgtactc gaatgttgat
      961 ttaagcgctt tgttcgtagt ggtagcgttg gtggtttcga ggttatttct tttttggtcg
     1021 gcttacaaaa ccgcgaatgc ctggtcgacc gtttcggctt ttaatatgtt aagcgttatc
     1081 ggttattttt tagtgccatt gacgctcgct ttggtttcgc ctgcgttgtt agtggggtcg
     1141 ttggacctac agaatatagc tttggcccag ggcggaatgc cgtgggaatg gatggttttc
     1201 aatggtccgg gcagcttttt ttcttttgct tcctttgcgg tagcgctttt tatttggcac
     1261 ggtcgcgcgc caagtaattt gttctttgaa agcacgtctg tgaaatcgga agtcatttcg
     1321 gaattcgaag ggcgcaggta ttttgttctc aagcagatag aggtgttttc cttatttcta
     1381 gcagcagcct tgattgtaac ggtttattta ggtgcttggc gggccccttt ttctttggaa
     1441 tccttcggac gggctgccaa tattgtagaa gcctgcattt tctttactaa aacaatgttc
     1501 gtaactttcg tgatgatttg gttccgttgg tctatgccga gcgcgcgact gaaacagata
     1561 tcaaatttaa gctggtactt gttattgccc gcaggctttg caggagttgc tatcaccttg
     1621 ctttggttag ctgtatctag aggagaaggg gtgcgttggt ggctttagct caaggtagag
     1681 tatttttcgg agttagaaag aatctcgcaa ccgcgtatcg tgttttgcga gtcacatttc
     1741 cctatctatt tggctatcgg agtgagtatc gagaaaacac cgaacagtac ccggatcgag
     1801 tcagcgcccg aatgccggaa gatttacccg ccaaagttag gggaatctta aaaaacgata
     1861 ttgagaaatg ttcagggtgt cgttactgcg ccgacgtttg tccggtaaat tgtatagaaa
     1921 tcgaaaccga agttggcccc gatcgcagtt tgagttgggt ttcagtcttt gatatcgatc
     1981 attctaagtg tattttttgt ggaatgtgtg ttgagtcctg tcccacagga agtttaattc
     2041 acaccaagga gtacgaagct tcgacggaaa aacttgattc gttagtaatg ggttttgggc
     2101 gcggctgggt tactcaaaat atgaaagacg catggaacag agaacaacag gctaaagaag
     2161 ctaaagccga agaagctgct gtgaatgagt tgagcccgat tagtgcagaa atcaagcgac
     2221 gactggaggg ctcatgacac ttttgggtct attgctctat ttgttgtttg ccaccactct
     2281 tttttgtgcg gtttatcttt tattctcaaa gaatttatac cgaatctcct ttgcgttatt
     2341 cgtgttgaac gtttccttgt cgggaataat gctttatttg ggctatcgcg ccatttcttt
     2401 aatatttttt tcctggtctg ctattgcgac cgttctgttt ctctcgtttt caagcgcgtt
     2461 ggtcggcgat ctttccaacg gttttcaaaa atctgaaggc actaaaaagt ttgcaaatta
     2521 tataggactt tttgttggtg gtggctttgg ttttggtttt ctatgggcga ttaagagaca
     2581 gggcttagca ctagatcgaa gtagttttga tccgggcatt ccgatggaag ccctaggaaa
     2641 gcaactgctc ggagagcact ccatcgtgct acatcttatt gggtttttga ttttaatcgt
     2701 ggtgataggg gcctctacat tgcggcgaag aattgatggg caacggagta gcaatgagtc
     2761 ctaacgtaaa tgacattcta gttttggcgg caattttatt gggattagga ataggcggtg
     2821 cgcttttcag aacaaatctt gttgtgttgt tcgcggcact tcaaaccgcg gctgctggcg
     2881 cggtattggc catatgtgca attggaacag ccagcgagac agctagaaat actgcacaga
     2941 tttttgcgtt aattgtaacc gtgtctatga gtatgagttc ggttttgggt tacattattt
     3001 tcttttctag gtttagagcc gtgaaacgca gcaatataaa cgatgccgtg gagttaagag
     3061 aataatgcta tc
//
LOCUS       DAOMXO010000236         3071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17904_length_3071_cov_5.387268, whole genome shotgun sequence.
ACCESSION   DAOMXO010000236 DAOMXO010000000
VERSION     DAOMXO010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17904_length_3071_cov_5.387268"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="PLH57_05880"
     CDS             complement(<1..1164)
                     /locus_tag="PLH57_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02177.1"
                     /translation="MVRKYFWSISVILGSLLAPSGFAQTPSLEQQCANQQTRDEALAV
                     GGDELARFNALCGHNRLASESINLLRMNRNYLSRVIEELEHFKAKGAGRHTSAKSYAE
                     YQDDGMCLQTEGIDRCIGRWKRARLDADAQIRRQVPKLNRARKELLSSQGQLEVLDVT
                     TAGATIEGGVLYDLYSKSRANAQSIRAKDIDRAAISSHGSQVYNSNESYNAVSDVDVT
                     DPHIALGLGIKGVPRPGIEVDSGARDEALNEAVEKVNQRVAAKYGLDTERATLAVQDA
                     ARQQKVYTGKLSAQIFKGLMDKMDVLIRDLDPKEYAGGLKKAPKGKIETLTIVNPITK
                     NAVNRSLANDPKNAQGSDPNSPGRTPAKPTEQQILDGPRFESLCAHHLSKGLSS"
     gene            complement(1553..1936)
                     /gene="rplQ"
                     /locus_tag="PLH57_05885"
                     /pseudo
     CDS             complement(1553..1936)
                     /gene="rplQ"
                     /locus_tag="PLH57_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(1940..3016)
                     /locus_tag="PLH57_05890"
     CDS             complement(1940..3016)
                     /locus_tag="PLH57_05890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRK02178.1"
                     /translation="MAKNWRDLIRPKALDVDRETLNSGYGKFVARPLERGFGITLGNA
                     LRRCLLSSLQGAAVTAVKIDGVLHEFTAIPDVNEDVTEIILNLKEVRFKMLNTDQATI
                     FINKTGAGDVCARDIMTGEQVQILNPDQVICSLGKDGKFKAEIIVRRGRGYATAETNK
                     TDDMGIGWIPVDSFFSPVRRVNYTVTQSRVGQRTDYDKLTLEVWTDGSVVPEDSVALA
                     SKILKEQLSVFLNFEEESEIIETAQEPESAEFNPNLYRSVDELELSVRSANCLQNASI
                     KYIYELVQRTEAEMLKTKNFGRKSLNEIKDILGEMGLSLGMKLDGFVPPTNPPKPADT
                     DVDENRILARKDSADLEDEEDDLE"
BASE COUNT          699 a          785 c          713 g          874 t
ORIGIN      
        1 ggagctaagc cccttggaaa ggtggtgggc gcacaaggac tcgaaccttg gaccatcgag
       61 aatctgctgt tcggtcggct ttgcgggcgt tctgcccggg ctgttgggat cgctgccttg
      121 agcattttta ggatcgtttg ccaaagaccg atttacggca tttttggtga tggggttcac
      181 gatggttagt gtttcgattt tccctttcgg ggctttttta agtccaccgg cgtattcctt
      241 ggggtccagg tcacgaataa gtacgtccat tttatccatg agtcccttga atatctgagc
      301 cgataatttt ccagtataga ccttctgttg gcgtgcggcg tcttggactg cgagggtcgc
      361 tctctctgtg tcgagaccgt actttgccgc aactcgttga ttcacttttt cgacagcctc
      421 gttaagcgct tcatcgcgag ctcccgagtc gacttcgatc cctggccttg gaacaccttt
      481 tataccaagt cccaaagcaa tatgtgggtc ggttacatcg acgtcagata cggcgttgta
      541 ggattcgttt gagttataga cctgggaacc gtgtgaacta atggctgcgc gatcaatatc
      601 tttagctcga atgctttggg cgttggctcg gctttttgaa tataagtcat acaagacgcc
      661 gccttcgatt gttgcaccgg ctgtagttac gtcgagcacc tcgagctgtc cttgggagct
      721 gagcaattcc tttcgggccc tattgagttt gggcacttgc cgccttattt gagcgtccgc
      781 atcgagacgc gcccttttcc accggccaat gcaacggtcg attccctcgg tttgcaggca
      841 cattccgtcg tcctgatatt cggcgtacga cttggcagag gtgtgacgac cagcgccctt
      901 tgctttaaaa tgttcgagct cttcgataac ccgactaaga tagttacgat tcattctcaa
      961 taggttgata ctctcacttg ccaggcggtt atgtccgcat agggcgttga agcgtgctaa
     1021 ttcgtcgcct ccgacggcta gggcctcgtc gcgggtctgt tggttcgcac actgttgttc
     1081 gagcgaaggc gtctgcgcga accccgaagg ggccagcaga gaacctagaa tgactgaaat
     1141 tgaccaaaag tattttcgga ccataacagt cgataattgt agcaaatttc aactagcttg
     1201 agaacctaga taggggtcta aataaacctt aaagggtcat attttcccta ttaatatcaa
     1261 ttagataaac aaaaaaggct gtttaacccg aagattaaac agcctttaac ttttgaaact
     1321 agcctaagct agcaagcaaa caggacgtcg gtaatcagct tgatccaccg cttcgtccac
     1381 cgcctgagct ttgggtcgaa gtttttcgaa ctgtagaggt ctttgcagct ttccctttag
     1441 tcgaaccacc agaaattttt ccagtggcgg aagctcgacc gtgtttcttt tcagggtggt
     1501 ggtgctcgtg cccctcgtgc ccttcgttct cttgagcttt atcgaatttt tttgctttct
     1561 tcttacgctc ggtaattggg cgatcgacaa gctctataat tgcgagctct gcgccgtcac
     1621 cccagcgggt gccgtcacgt cgaagaacgc gagtgtaacc accagcgcgc ttcgcataac
     1681 gctctgagag cgacgtgaat agcttattca cggcttcttc gtctctagtt cgcgcaaaag
     1741 ccaaacgacg agcgtgcagg gtgccttctt taccaagagt aatcaggcga tccactacgc
     1801 gacgaatttc ttttgccttc gcaacggtag ttgtcacctg ctctttgcaa atcaagttta
     1861 ctgcgagatt acgaaacatc gccaatcggt gcgatgtatt catcccaaat tttcggccgt
     1921 ctactaaatg tctcatgaac tactccagat cgtcttcttc gtcttcgagg tcggccgagt
     1981 ctttgcgtgc taagattcgg ttctcgtcta cgtcggtatc agcaggcttt ggtgggttgg
     2041 taggaggcac gaaaccatcc aacttcattc ccaagctcaa acccatttct ccgagaatgt
     2101 ctttaatttc gttcaatgac ttgcgaccga aattcttagt cttaagcatt tcagcttcgg
     2161 tacgttgaac taattcatag atgtacttga tgctcgcatt ctgcaaacaa tttgcagagc
     2221 gaactgacaa ctcaagttca tccactgagc ggtagaggtt cggattgaac tctgcgcttt
     2281 ctggctcttg agcagtttcg ataatttcgc tctcttcttc aaaattaagg aataccgaaa
     2341 gttgttcttt cagaatcttc gatgcaagcg caacagaatc ttctggcaca accgatccat
     2401 cggtccacac ttcgagagta agtttatcgt agtcagtacg ttgtccgacg cgcgactgag
     2461 tcactgtgta gtttactcgg cgcactgggg agaaaaaaga atcgaccgga atccagccaa
     2521 tacccatatc atcggttttg ttcgtttcag cggtcgcata accacgcccg cgtcgtacga
     2581 tgatctccgc cttgaatttc ccgtcttttc caagagagca aatgacttga tcggggttca
     2641 ggatctgcac ctgttctccg gtcatgatat cacgtgcgca aacgtcacca gcaccagttt
     2701 tattgataaa aatggtagct tggtccgtat tcaacatctt gaatcgcact tccttaagat
     2761 tcaaaataat ctcggtcacg tcttcgttca catctggaat cgctgtaaac tcgtgcaata
     2821 ctccgtcaat ttttacagcc gtcactgccg cgccctgaag cgaagacaaa agacatctgc
     2881 gaagcgcgtt cccaagagtg ataccaaatc ccctctcaag tggtcgtgct acgaactttc
     2941 catatcctga gtttaacgtt tcccgatcga cgtctaacgc cttcggtcta attaaatccc
     3001 gccaattctt cgccatgaaa cctgtcacgc tcgacatttc aatctcctca aatcaaaaat
     3061 tacttggagt a
//
LOCUS       DAOMXO010000237         3067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_17946_length_3067_cov_3.722776, whole genome shotgun sequence.
ACCESSION   DAOMXO010000237 DAOMXO010000000
VERSION     DAOMXO010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17946_length_3067_cov_3.722776"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="PLH57_05895"
     CDS             <1..1097
                     /locus_tag="PLH57_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HRK02179.1"
                     /translation="DHARFRNIVKGKIKQDLKKYISHGEMIGRKGKDMVTIPLPQIDI
                     PHFRYGDKEAGGVGQGDGKPGDAIGQGEPQEGDGEGKAGQNAGEHSLEVDVSIQELAE
                     ILGEELQLPKIEPRGHKTVKVVKDKYTGIARVGPDSLRHFKRSFKEALKRQISLGQYD
                     SKSPVIVPIRRDLRYRSWRSVVEHENNAVIIYMMDVSGSMGDEQKEIVRTEAFWIDTW
                     LRSQYHDIETRYIIHDATAKEVDEDTFYKTRESGGTLISSAYKLCQKMIETDYAPSEW
                     NIYPFHFSDGDNWSGEDTKVCMDLLNNDILPRVNVFCYGQVESRYGSGQFFKDLREEF
                     EESDDRVILSKIENKEGIIQSIKEFLGKGK"
     gene            1102..2580
                     /locus_tag="PLH57_05900"
     CDS             1102..2580
                     /locus_tag="PLH57_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HRK02180.1"
                     /translation="MGLPLELAKLRDEIRGYAVSYGLDFFETVFELLEHDQLNAVAAL
                     GGFPVRYPHWRFGMEYDQLSKTYTYGLQKIYELVINNDPSYAYLMKSNAMIDQKLVMA
                     HVYGHVDFFKNNTWFSKTNRKMIDTMANNAARIREFMDRHGVDRVENFIDRCLSLENL
                     IDPFLPFTPKPNAKNKEDNDDSGNIPGRIKVQRGYMDTFINPPDFIEDQKRKLAQQEV
                     KSKRNPSEPQKDVLAFLLDNAPLETWEHDVLAMIRDEAYYYAPQWQTKILNEGWASFW
                     HSRILTEKALRDSEVIDYADHHSGTMAMQPGRINPYKIGIELLRDVEDRWNRGKFGKD
                     YEQCDDIVAKRQWDKKLGLGKEKIFEVRRVCNDVTFIDEYLTPEFCERFKLYTYQFNR
                     RTNQYEIADRDFKKIKEKTLFGLTNMGQPFIYVADGNYLNKGELLMLHKHMGVDLDSR
                     YTRETMRNLSEVWRRPVNLETEVDGQRKLFMYSNGEFSEKFL"
     gene            complement(2590..>3067)
                     /locus_tag="PLH57_05905"
     CDS             complement(2590..>3067)
                     /locus_tag="PLH57_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02181.1"
                     /translation="FGSRAEGAGLWLGGQGGIVKANSSQFTVYGATVGVMPTRSYSLG
                     AFYLVSNTKTASVGPVTAETKLAFYGGDFSLHLDPMGVTRIGIKGGLSKVEGKARVLG
                     VSVGASSTDLAIGPSIGFDLKLGSGLSIGAEGAYLRVMADDSYSALLALGALKLWF"
BASE COUNT          894 a          683 c          761 g          729 t
ORIGIN      
        1 gcgaccacgc tcggtttcgc aatatcgtca aaggtaagat caaacaggat cttaaaaagt
       61 acatttcgca tggcgaaatg atcggtcgta aaggcaagga catggttacc attcctctgc
      121 cccaaataga cattccgcat tttcgctacg gcgataaaga ggctggtggt gtggggcagg
      181 gcgatggcaa accgggcgac gcaatcgggc aaggcgagcc ccaagaaggc gacggagaag
      241 gaaaagccgg ccagaatgct ggagaacaca gtctagaagt agatgtgtcg attcaagaac
      301 tcgcagaaat tctgggcgaa gaattgcagc ttccaaaaat tgaaccccgc gggcacaaaa
      361 cggtaaaagt ggtcaaagac aaatacaccg gcattgcacg ggttggcccc gacagcttgc
      421 gccatttcaa acggtccttc aaagaagcgc tcaaacggca gatctcattg ggccagtacg
      481 attcgaagtc tccggtgatt gtgcccattc gaagagatct acgatatcgc agctggcgct
      541 cggtcgttga acacgaaaat aacgcagtga ttatttacat gatggacgtg tcgggatcga
      601 tgggcgacga acaaaaagag atcgtgcgaa ccgaggcgtt ttggatcgat acatggctac
      661 gcagtcagta tcacgacatc gaaacgcgtt atattatcca cgacgctacc gccaaagaag
      721 tggacgaaga cacattttac aagactcgtg aaagtggcgg caccctgatt tctagcgcct
      781 acaaactttg tcaaaaaatg attgaaacag attacgcccc gagtgagtgg aacatttatc
      841 cgtttcattt ctcagacggt gacaattggt cgggtgaaga tacgaaagta tgcatggatc
      901 ttttgaataa cgacatcctg ccgcgggtga acgtgttttg ttacggccaa gtggaaagtc
      961 gttacggcag cgggcagttt tttaaagacc tgcgtgaaga gtttgaagag tctgacgacc
     1021 gagtgatact ttccaaaatt gaaaataaag aaggcatcat tcaaagcatt aaagaatttt
     1081 tagggaaggg aaaataacca catgggttta cctctcgaac ttgctaaatt gcgtgacgaa
     1141 attcggggat atgcggtttc ctacggtctc gatttttttg agacggtttt cgaattgctg
     1201 gagcacgacc aactcaacgc agtggcggca ctcggtgggt ttcctgtgcg ctatcctcac
     1261 tggcgtttcg gaatggaata cgaccaactt tctaaaacct atacctatgg gttgcagaaa
     1321 atatatgaac tcgtaatcaa taacgacccc tcctacgcct atttaatgaa atcgaacgcg
     1381 atgatcgatc agaagttggt catggcgcat gtctacgggc atgtcgattt ttttaaaaac
     1441 aatacgtggt tttctaaaac taatcgcaaa atgatcgaca ccatggccaa taatgccgcg
     1501 cgaatccggg agtttatgga tcgacatggc gttgaccggg tggaaaattt tattgatcgc
     1561 tgtctttcgt tggagaattt gattgatccg tttttgcctt tcacgcccaa gcccaacgct
     1621 aaaaataaag aagacaacga tgactcggga aatattcctg gccgcatcaa agtccagcgc
     1681 ggatacatgg atacgtttat taatccgcca gattttatcg aagatcaaaa acgaaaactt
     1741 gcgcagcagg aagtaaaatc aaaacgtaat ccaagtgaac cccaaaaaga cgttctagcc
     1801 tttctgctcg ataacgcacc tctagaaact tgggagcacg atgtgctcgc catgattcgg
     1861 gatgaagcgt attattatgc tcctcagtgg caaacaaaga tattaaatga aggttgggct
     1921 tcgttttggc attcaagaat acttaccgaa aaagcgctac gtgattcgga agttattgat
     1981 tacgctgacc atcattccgg taccatggcc atgcaaccag gtcgtattaa tccgtacaag
     2041 attggtattg agttgctgcg cgacgtagaa gatcgatgga atcgtgggaa gttcggtaaa
     2101 gactacgaac agtgcgacga tatcgtagct aagcgccagt gggacaagaa gcttggacta
     2161 ggtaaagaaa agatcttcga agtacggcgc gtttgtaatg acgtgacatt tatcgacgag
     2221 tacttaacgc ccgagttttg tgagcgcttc aaattgtata cctatcaatt caatcgccgc
     2281 accaatcagt atgaaatagc tgatcgcgat tttaagaaga taaaagaaaa aaccctcttc
     2341 ggtttgacca atatgggcca gcccttcatt tacgttgccg atggtaacta cttgaataaa
     2401 ggagaactgc tcatgctgca caaacacatg ggcgttgatt tggattcgcg gtatactcgc
     2461 gagaccatgc gcaatctttc cgaagtgtgg cgccggccgg tgaatttaga aactgaagtt
     2521 gatggtcaac gcaaattgtt catgtattcc aacggtgaat ttagcgaaaa atttctctga
     2581 gattttgcgc tagaaccaga gtttaagggc tccaagtgcg agtaaagccg agtacgaatc
     2641 gtcggccatg acccgcaaat acgcgccttc ggcgccaatg ctgagcccgg agccaagctt
     2701 caaatcaaaa ccaatcgatg gtccaatggc gagatcggtt gaagaggctc caaccgaaac
     2761 tccaagtact cgcgctttgc cttcaacctt acttaaacca cctttaattc caattcgagt
     2821 tactcccatc ggatcaaggt gaagggaaaa atctccgcca taaaacgcta gtttggtttc
     2881 tgcggtcact ggtccgaccg atgcggtttt tgtattggaa actaaataaa atgcgccgag
     2941 cgagtacgaa cgagtgggca tgacccctac ggtagcaccg tatacggtga attggctgga
     3001 gttagctttg acgatcccgc cttggccgcc cagccagaga ccggcgcctt cggcgcgaga
     3061 accaaaa
//
LOCUS       DAOMXO010000238         3059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18016_length_3059_cov_5.183422, whole genome shotgun sequence.
ACCESSION   DAOMXO010000238 DAOMXO010000000
VERSION     DAOMXO010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18016_length_3059_cov_5.183422"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /locus_tag="PLH57_05910"
     CDS             <1..1537
                     /locus_tag="PLH57_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRK02182.1"
                     /translation="ISMKGGIRKFFCGPESFTPDLRPIVGEAPELQNYFVAAGLNSVG
                     ILTAGGIGRVLAHWMDTGVPDVDVTGINIDRLTKYQSNPQYRRERTVESLGMVYRCHY
                     PTMSYQTARGAKKSPFHDRLAAAGAYFKDVSGWEGADWYAPKGHEPKVEKLSWGRQNW
                     FNWWKEEHQAAREGVIVMDMSFMCKFLVQGRDAGRVLNQISANNVDGPSETITYTQWL
                     NREGKLEGDLTVAKLSDDRFMVVVTDTMLRHAETWMKRNIPSDAYASVTDVTGAYGQL
                     NIQGPRSRELLQSLTTADLSNEAFPFRTAREIDIGYARVLCVRITYLGELGYELYIPS
                     EQATHVYDRIVEAGGSYGLRHAGLKALGSLRMEKGYRDYGHDIDNTDDPLETGLGFAV
                     DFKKPDGFIGREALVKKKEAGLASLTRRLVQVLIKDPEPMMFHAEIVRRNGRAVGYVR
                     SASYGHTLGGAVGLAMIEAGEPMDANYIASGTWEVEIANKLWPATVSLRPLYDPEMKK
                     IKA"
     gene            1628..2515
                     /locus_tag="PLH57_05915"
     CDS             1628..2515
                     /locus_tag="PLH57_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02183.1"
                     /translation="MPDMFSLRIFAAFLFVCWPLGSFSEELPCKSHELGCDEKFFSHY
                     QALNGALDAAISANPALVEELGEEVETAYHSVWLQRFESGAIFHISQRVPFGAVKLES
                     HEGGNFILEQSSNQFLFHWRPPVPGIYDVDLLTAIMKYYPRDPDLFLNWSIGHTLSET
                     EIADFFRPHTESSPRNISGGLATLYAMYRLDQYLGRPLEQQFTRVVLRRNYKAGYVLV
                     GLPDPAASALAGDATEALAEDVNHLYIFKIEREPIGSTDTDRYGGGKKFRAGSWKTAK
                     IDLGEDEKNFRPKRGLFSE"
     gene            2540..3025
                     /locus_tag="PLH57_05920"
     CDS             2540..3025
                     /locus_tag="PLH57_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02184.1"
                     /translation="MLRNFGLLMLVLASNDLVWASTAIRDYEPIGITQGGDRILETVF
                     DAGQARKIYEGLKLDESLDDPATTMCLAAEEENGCMKISEKRIEGAARVNCLRFHYPS
                     LLTKANRDSFNEIWTKEFKSIPEQDAFLCVVATRYRSSQFKKRAEEYLRVGGAKFLPA
                     D"
BASE COUNT          766 a          703 c          838 g          752 t
ORIGIN      
        1 gatttctatg aaaggcggta ttcgaaaatt tttctgtggt cctgaaagct ttacgccgga
       61 cttgcggccc atcgtcggag aagccccaga gttacaaaac tatttcgtag ccgcggggtt
      121 gaattctgtg ggtattctta cggcaggcgg aattggtcgt gtgctagccc actggatgga
      181 cactggtgtg cccgatgtcg acgtgactgg gattaacatt gatcgtttga cgaaatatca
      241 atcgaatccc cagtatcggc gcgaacgtac ggtggagtcg cttggcatgg tgtatcgttg
      301 ccactatccg accatgtcgt accaaacggc gcgcggagcg aagaagtcgc cgtttcatga
      361 taggctcgcc gctgctggcg cttatttcaa agacgtgagc ggctgggaag gcgcggattg
      421 gtacgcacca aagggccatg aaccgaaagt cgaaaaactt tcgtggggcc gtcaaaattg
      481 gttcaattgg tggaaagaag aacatcaagc ggctcgcgaa ggcgtgatcg tgatggacat
      541 gtcgtttatg tgtaaatttc tggtccaagg tcgcgacgcc ggccgagtgt taaatcagat
      601 ttcagcaaat aacgtagacg gcccgagtga gaccatcact tatacccagt ggttgaaccg
      661 cgagggtaaa cttgagggcg atctcacggt tgcgaagtta agcgatgatc gcttcatggt
      721 cgtggtcacc gacaccatgc ttcgccacgc ggaaacttgg atgaaacgca acattccaag
      781 cgacgcatac gcgtcggtaa ccgacgtgac aggtgcctac ggccaactga acattcaagg
      841 cccgcggtct cgtgaattat tgcagtcttt aacgacggcc gatctttcga acgaagcgtt
      901 tccattccga accgcacgtg aaatcgacat cggttacgcg cgggtgctgt gtgtgcgaat
      961 cacgtatttg ggggaactcg ggtatgagct ttacattcca tcggagcaag ccacccacgt
     1021 ttatgatcgc atcgtagaag cgggcggttc gtatgggttg cggcacgcag gactcaaggc
     1081 gctcggaagt ttgcgtatgg agaagggata ccgcgactat ggccacgata tcgacaacac
     1141 tgacgatccg ctcgagaccg gtctcgggtt tgcagtggat ttcaaaaaac ccgatgggtt
     1201 tattggacgc gaagcgctcg taaaaaagaa agaagcaggc ctggcttcgc tcacgcgaag
     1261 gctcgtgcaa gtgttgatta aagacccgga gccgatgatg tttcacgctg agatcgtgcg
     1321 gcgaaatggc cgcgccgtcg gttatgtaag atcagcttct tacgggcaca cgttaggtgg
     1381 agcagtgggt ttggccatga ttgaggccgg tgaacccatg gacgcaaact acattgcaag
     1441 cggcacctgg gaagtggaaa ttgccaacaa actctggcct gcgactgttt cgttgcgacc
     1501 gctatacgat ccggagatga agaaaattaa agcctaagat ttcttgaacg attctttgtt
     1561 taatccatga atacgagggc ttgtagaata cgttgacgca ttgcgtttta aggtgttttt
     1621 ccatataatg cccgacatgt ttagtttacg gatttttgcc gcgtttctct ttgtttgctg
     1681 gcccttgggt tccttcagtg aagagctccc ctgcaagagt cacgagctcg gttgtgatga
     1741 aaagtttttt tctcactacc aagcgttgaa cggggcgctt gatgctgcta tttcagctaa
     1801 tcctgctttg gtcgaagagt tgggtgaaga agttgaaacc gcctaccatt cagtgtggct
     1861 gcagcgtttt gagagtggtg cgatttttca catttcccaa agagtacctt ttggcgcagt
     1921 aaaacttgaa tcccatgaag gcggcaattt tattttggaa caatcgagca accagtttct
     1981 gttccattgg cgcccaccag tgccgggaat ctacgacgtc gatttattaa ctgcgatcat
     2041 gaagtactac ccgcgggacc ccgacctgtt tttaaattgg tcgatcgggc acactctgtc
     2101 ggaaacagag atcgcagatt tttttcggcc gcacactgaa agttcgccca gaaacatcag
     2161 cggtggtctc gctacgttgt atgccatgta tcgcttagac caatatcttg gtagaccact
     2221 tgaacaacag ttcacacgcg tcgtattgcg acgcaattac aaggcaggct acgtcctcgt
     2281 aggtttgccg gatccggcgg cttctgcatt agctggcgat gctaccgaag cattggcaga
     2341 ggacgtgaac cacctgtaca tttttaaaat cgaacgagaa cccataggta gtaccgacac
     2401 cgatcgctac ggcggtggaa aaaaatttcg cgcgggcagc tggaagaccg caaaaattga
     2461 tctgggtgag gacgaaaaga attttcgtcc gaagcgcgga ttatttagcg aataggtaaa
     2521 agaaaactag gagtcgaatg tgctaagaaa ttttggtttg ttaatgttgg ttctcgcttc
     2581 taacgatctc gtctgggcgt cgaccgccat tcgcgattat gaaccgattg gaatcaccca
     2641 agggggcgac cgtatactag aaacggtgtt cgatgcggga caagctcgta aaatatacga
     2701 gggcttgaaa ctagatgaat cgctcgacga cccagctaca accatgtgtc ttgccgcaga
     2761 agaggaaaat ggttgtatga agatctctga aaaacgcatc gaaggggctg cccgtgtgaa
     2821 ttgccttcgg tttcattatc catcactact cactaaagct aaccgcgact cattcaatga
     2881 gatttggact aaagaattta agtcaattcc agagcaagat gcctttttgt gcgtggtcgc
     2941 aacgcggtac cgttcaagcc aattcaaaaa aagagcggaa gaatatttaa gggtcggtgg
     3001 cgcaaagttc cttcccgcgg actgagcgct ttaagtcgaa cgtcgcgaat tgcgactgc
//
LOCUS       DAOMXO010000239         3057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18031_length_3057_cov_4.724850, whole genome shotgun sequence.
ACCESSION   DAOMXO010000239 DAOMXO010000000
VERSION     DAOMXO010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18031_length_3057_cov_4.724850"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLH57_05925"
     CDS             <1..577
                     /locus_tag="PLH57_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02185.1"
                     /translation="ALLINGPERVFLEAIRSGQSVKFKARPGFARDAQQIHGYILGAS
                     RSMFLRDLEVIVQEDMGPFQTLGVRSFKVKHIDFDSIELVTPETPTLDSQEESLLRLL
                     KSSLGVDAAPVWILIKNPKVTPVMGPVFLTNDYYAHQPVHVVGVRRSASGQTYMVDVL
                     VNKGDKSWTDSLDFRTIDTIGTRIQKTNSAK"
     gene            complement(561..1943)
                     /locus_tag="PLH57_05930"
     CDS             complement(561..1943)
                     /locus_tag="PLH57_05930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK02186.1"
                     /translation="MRHQNPQPCIQFLGGVGTVTGSKFLVSLGSEKILIDCGLFQGLK
                     ELRLQNWAEFPIDPRSITAVVLTHAHLDHSGYLPLLVKKGFAGKIYCTPPTRDLSKII
                     LEDSAEIQEEDADYANKKGYSKHDPAKPLYTIEDVRRSLRHFTVVPNSDWFKINDRIK
                     FRFKPNGHILGSAFIELKIGTTKITFSGDMGRKNPIILNPSSSPEKTDFLIVESTYGD
                     RLHPNLSPARELKRIINETLARKGKLIIPSFAVGRAQDLLHLLSQLRAQNDIPNVPIY
                     LDSPMGIHATDIFMAHSKWHKLKNNEVDALGRIAVMVRKQRNSIEIAEKKQSSIIIAG
                     SGMLSGGRVLHHLEKCLPDERNTVLIVGFQAASTRGRLLKEGIPELKIHGKYIPVHAR
                     IEEISGLSAHGDQKEILDWLKQFKTAPKNTFIVHGEPQASDALRVCIQDRLGWNCTIP
                     KPFDHFILPN"
     gene            complement(1968..2738)
                     /locus_tag="PLH57_05935"
     CDS             complement(1968..2738)
                     /locus_tag="PLH57_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK02187.1"
                     /translation="MREINYQFDGKVALVTGAASGIGRATAIAFAKSGANVVVADVLE
                     KGGTETASMIDALGRKALFVRCDVSDTQNVKDLVRRTVEKFGRLDFAFNNAGIEDTPA
                     STTECTEDNWHKTIDINLKGVWLCMKYEIPELLKQGGGAIVNCSSIAGLIGFSGIPAY
                     TASKHGVIGLTKTAALEYSKSSIRVNAVCPGVIQTPMIDRFTQGSAEAKAQLTAGAPM
                     GRVGLPEEIANAVLWLCSDSASYMTGQTMTIDGGWVAQ"
     gene            complement(2840..>3057)
                     /locus_tag="PLH57_05940"
     CDS             complement(2840..>3057)
                     /locus_tag="PLH57_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HRK02188.1"
                     /translation="NSFMKKIHDRMPVILDREMEKVWLDPTNHDTLHLKTFLKPCPSN
                     WLSAHEVSSLVNSPKNNRKEILKPYAR"
BASE COUNT          782 a          650 c          755 g          870 t
ORIGIN      
        1 ggcgttattg attaatgggc cggaacgggt gtttttggag gccattagga gtggtcagtc
       61 cgtaaaattt aaagcccggc cgggatttgc acgggatgca caacaaattc acgggtacat
      121 tcttggtgcg agcaggtcaa tgtttcttcg agatcttgaa gttatagttc aagaagacat
      181 gggcccgttt caaactcttg gcgttcgttc atttaaagta aaacacatcg attttgattc
      241 gatagaattg gttacgccag aaacaccaac actcgactcg caagaagaat cattactgcg
      301 actactgaaa agttctctag gagtggacgc tgcaccggtg tggatactaa tcaaaaatcc
      361 aaaagttact cctgtgatgg gtccagtgtt tctgacaaac gactactatg cgcatcaacc
      421 ggttcatgtg gtcggagttc gaaggagcgc ctcgggccaa acatatatgg tcgacgtgtt
      481 ggttaataaa ggcgataaaa gctggaccga tagtttagat tttcgaacaa ttgatactat
      541 tggtactcga atacaaaaaa ctaattcggc aaaatgaaat gatcgaacgg ttttggtatg
      601 gtgcagttcc acccgaggcg atcttgtatg caaacgcgta gtgcgtcgga ggcctgcggt
      661 tctccgtgaa caataaaagt atttttgggt gcagttttga actgtttcaa ccaatcgagg
      721 atttcttttt gatccccgtg tgccgagaga cccgaaattt cttcaatgcg tgcatgtacc
      781 ggtatgtatt tgccatgaat tttaagttcg ggaatacctt ctttaagtaa gcgaccacga
      841 gtgctcgccg cctggaagcc gacaattaga acagtgtttc gttcatccgg taggcacttt
      901 tccagatggt gaagcactcg gccgccagat agcattccgc tcccggcgat tatgatggag
      961 ctctgttttt tttcggcgat ttcgatggag ttgcgttgct tccgtaccat gactgcaatt
     1021 ctgcccaagg catcaacttc gttgttttta agtttgtgcc attttgagtg cgccataaaa
     1081 atgtcggtgg cgtgtattcc catgggacta tcaaggtaaa ttggaacatt cggaatatcg
     1141 ttttgggctc gcagctgaga caataggtgt aataggtcct gggcacgacc cacggcgaag
     1201 ctcgggataa taagtttacc ttttctcgct agggtttcgt ttattattct ctttaattca
     1261 cgcgctggtg aaaggtttgg gtgaaggcga tctccgtatg tcgattcgac aattaaaaaa
     1321 tcagttttct cagggctaga cgacggattt aaaataatag gatttttacg acccatatcg
     1381 ccggaaaaag taattttcgt tgttccgatc tttaattcaa tgaaggctga accaagtatg
     1441 tgaccgttcg gtttgaaacg aaatttaatc cgatcgttaa ttttaaacca atcagagttc
     1501 ggtactacgg taaagtggcg aagcgatcgc cgaacatctt caattgtata gagaggtttt
     1561 gccgggtcat gtttggagta gcctttttta tttgcataat ctgcatcttc ttcctgaatt
     1621 tcggcagagt cttctagaat tatcttcgac aaatctcgag tgggtggcgt gcagtaaatt
     1681 tttccagcaa aacctttttt aactagtaat ggcaaataac cggagtgatc gagatgagcg
     1741 tgggtgagga ctacagcggt aatagagcgt ggatcgatcg gaaattccgc ccaattttgc
     1801 aaacgtagtt ctttgaggcc ctgaaacagt ccgcaatcga taagaatctt ttcggaacct
     1861 aaacttacca aaaacttgga gccggtgacg gtaccgaccc cgcctaaaaa ttgaatgcaa
     1921 ggctgggggt tttggtggcg catttaaaaa tggtccttgg ctctgcgtta ttgggcaacc
     1981 catccgccgt cgatggtcat ggtctggcct gtcatgtaag aggcagaatc tgagcaaagc
     2041 cataacactg cgttggcaat ttcttcgggt agtccaacgc gccccatagg cgcaccagca
     2101 gttagttgtg ctttcgcttc cgccgagcct tgggtgaaac gatcaatcat aggtgtttga
     2161 atcacacccg ggcatacagc gtttacgcga atggaagact tggaatactc taaagcagcc
     2221 gttttggtaa gaccgatgac gccgtgtttg ctggctgtgt acgcgggaat tcccgaaaac
     2281 ccgattaaac cggcaatcga ggagcaattt acgatagcgc cgccaccttg tttcagtagt
     2341 tcggggattt cgtatttcat gcacaaccaa acgcctttta aatttatatc gatggtttta
     2401 tgccagttgt cttcagtgca ttcggtagta gaggccgggg tgtcttcgat gcccgcattg
     2461 ttaaatgcaa aatccaacct gccaaatttt tccacagttc gtcgcacaag gtccttaacg
     2521 ttctgggtgt cggaaacgtc gcaacgtacg aaaagggctt tgcgtcccaa ggcgtcgatc
     2581 atgctggcag tttcagtgcc acccttttcc aagacgtcag ctacgacaac gttggcgccg
     2641 cttttagcaa atgcaattgc agtggctcgg ccaattcccg acgctgcacc agtgacgagt
     2701 gcaacttttc cgtcgaattg atagtttatt tcgcgcatga tcggcctcac tttcaagttt
     2761 ggcgtcacat taaccccaat tgagatttag ttcaaatagg gcgttggaag tacgattatg
     2821 agtctcggac aaagagttcc tagcgggcat aaggtttcaa aatctctttt cggttgttct
     2881 ttggtgaatt aaccagacta gacacttcat gagcagagag ccaatttgaa ggacaaggtt
     2941 tcagaaaagt ttttaaatgt aaggtgtcgt gatttgtggg atctagccag accttttcca
     3001 tttcgcggtc gagaatcacc ggcatgcggt catgaatttt tttcatgaac gaattgg
//
LOCUS       DAOMXO010000240         3055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18053_length_3055_cov_4.471333, whole genome shotgun sequence.
ACCESSION   DAOMXO010000240 DAOMXO010000000
VERSION     DAOMXO010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18053_length_3055_cov_4.471333"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLH57_05945"
     CDS             complement(<1..183)
                     /locus_tag="PLH57_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02189.1"
                     /translation="MKTLTEFDGFTLKRGLQKLAELEKEGKTAEEIPALITESFKYEG
                     DKLKFFQVALEMAKARG"
     gene            complement(295..2493)
                     /locus_tag="PLH57_05950"
     CDS             complement(295..2493)
                     /locus_tag="PLH57_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02190.1"
                     /translation="MGLLRRFIFIAGFAVASTLGQASEVGCDIYLILSELTIKVEEGA
                     GFRVVGRTADGKHGTLIVELGADKKRKFFKTTSSENQEIVAALFGDGHGEGAMADREF
                     ASSRLFAHVGWEGSTRVRRAELKMRDGRVEKGVLVDEVPGESLATIQKRDRGAYNKIL
                     RSPEYKQAAEEATLIQFVTGNIDWISYREGQGIDLLSENVMVFEDPVTKRYSIRFIDN
                     GASFTKTQAVDAAATPREGLSLGERLKRLQLLHVQFEKKLPQVFYPLLDLEDMSSTTQ
                     KLLIKLAEFSDEELTQLLAKSPTLNDNDINGAIARIRYLEERFIERRPEIQMARNDYP
                     PDINVRDNDDITSGRAQREATVDLELETPIVGESPAKGRLPVKSDFTDDPSVRIVGEV
                     ARVQKKNKNVVYKVEIDGQTHYFKPKSGENPLIGESFLGKFEKGMMADREVFASLVYQ
                     KAGWSEVPRVKKVKIKVDGELEEGALVQGLPGKTIREFGTKRFDEMIEGQADYRRSRE
                     RALILSVVTGNEDWYRIKSNRNAHENAGNLLLEVDPEDPLLYNYYWIDSGLSFPRRSR
                     RSNTRLPGAQSSQYLAEQFGHLWPLLLDLDTLDSGTIDLIKELSKVSFVEWRELAKEL
                     PTITQTDIEGMMIRVNYLNRRFVLNDPEFQVTQPMREAWHKKRVSKRILPGRTVKADS
                     DDTTLTPTQRRTDTHQVDTSESTQSAFDNEPTKAGPRPGDNDGTPDHRAR"
     gene            2601..>3055
                     /locus_tag="PLH57_05955"
     CDS             2601..>3055
                     /locus_tag="PLH57_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK02191.1"
                     /translation="MSGLKRIVITGGPSAGKTTLLDLLQKQYGDRVAIAPEAASMLLR
                     GGFMRPDTDADRVHTQRAIFSLQRELEAKAIAHCATTHGVCETKVSLFCDRGSMDGAA
                     YWPGTAESFVAEMGTTVEHEYSRYSLVLHLQSAEEGRGYTRTEIRTESAS"
BASE COUNT          687 a          812 c          728 g          828 t
ORIGIN      
        1 accgcgagcc tttgccattt caagagctac ttgaaaaaac ttaagtttat caccttcgta
       61 tttaaaactt tccgtgatca acgctggaat ctcttccgca gtttttcctt ctttttccag
      121 ttcggccaac ttctgaagac cgcgttttaa agtaaatcca tcgaactcag tgagagtttt
      181 catcgtttcg ctcctaatag catcgccgta aaggcccgct atatcgtggt cgcgaaccga
      241 tcgcaagggc actttgactc ggcgcaattg tttttgaatc actcaacctg gttactatcg
      301 ggccctatga tccggcgttc cgtcgttatc ccccggtcgt ggccccgcct tagtaggttc
      361 gttatcgaac gctgactgag tggactcgga ggtgtccact tggtgggtat ctgttcgccg
      421 ttgggtagga gttaaggtcg tatcgtcgga atcggcctta acggtacgcc ccggaagtat
      481 tcgcttcgat actcgtttct tatgccaagc ctcgcgcatt ggctgggtca cttgaaattc
      541 ggggtcgttt agaacaaatc gtcgattcaa atagttcacc cgaatcatca ttccttcgat
      601 atcggtttgg gtaatggtcg gcagttcttt cgctaattcg cgccactcta cgaaactcac
      661 cttggagagt tctttgatca aatcgattgt tccggagtcg agcgtatcga gatcaagcaa
      721 cagcggccac aggtgtccga actgttcggc caaatactgg gaagactgcg caccaggcaa
      781 gcgggtattg ctgcgccgac tacgacgggg aaacgaaagc ccactgtcga tccagtaata
      841 gttatataga agcgggtctt cggggtcgac ctccaacaag agattgccgg cattttcatg
      901 tgcgtttcga ttggatttaa ttcggtacca gtcttcatta ccggtaacaa cagacaggat
      961 cagcgcccgc tcgcgcgaac gtcgatagtc cgcctgacct tcgatcattt catcaaacct
     1021 tttggttccg aactcgcgaa tggttttgcc aggcaaccct tggactagag caccttcttc
     1081 gagttcaccg tctactttga ttttaacttt ctttactctg gggacctcgc tccaaccggc
     1141 cttttggtat accaacgacg caaaaacctc gcgatcggcc atcatacctt tttcgaactt
     1201 tcctaagaaa ctctcgccga ttagtggatt ctccccagat ttgggtttga agtaatgagt
     1261 ctgaccatcg atttcaacct tgtagaccac gttcttgttt tttttctgaa cgcgagcaac
     1321 ttcgcccacg atgcgcacag atggatcgtc ggtaaaatca ctttttacgg gaagacgacc
     1381 cttggccgga ctttctccga caatcggtgt ttcgagctcg agatcgacag tcgcttctcg
     1441 ctgcgcccgc ccgctggtga tgtcgtcatt gtcgcgaacg ttgatatccg gcggataatc
     1501 gttgcgagcc atctgaattt cgggccgccg ctcgatgaaa cgctcttcga gatatcgaat
     1561 ccgagcaatc gcgccgttga tatcgttatc atttaaggtc ggacttttag ccaagagctg
     1621 ggttaattct tcgtctgaaa actccgctaa ttttataagc aatttctgag tcgtactact
     1681 catgtcttcg aggtcaagca gtggataaaa cacttgaggc aatttttttt caaactgtac
     1741 gtggagcaat tgtagccgct tcaagcgctc acctagggaa agaccttcgc ggggagtagc
     1801 cgctgcatca acagcctgag tttttgtaaa agaggcgcca ttgtcgataa atcgtatcga
     1861 atagcgtttg gtcacggggt cttcgaagac cattacgttc tcggagagca aatcgatgcc
     1921 ctgtccttcc cgataagaga tccaatctat atttcctgtt acaaactgaa tcagagtggc
     1981 ttcttctgca gcctgtttgt attcgggtga tcgtagaatt ttattgtagg caccacgatc
     2041 acgtttttga atggttgcga gcgattcacc cggtacttcg tcgaccaaga cgcccttttc
     2101 gacccgtccg tcgcgcattt ttaattccgc gcgccgtact cgggttgagc cctcccatcc
     2161 aacatgggcg aaaagccgac tgctcgcaaa ctctcggtcc gccatggccc cttccccgtg
     2221 cccgtcccca aaaagtgccg ctacgatttc ttggttttca gagctggtgg ttttgaagaa
     2281 ttttcgtttt ttatcggcgc ccaactcaac aattagggtt ccgtgtttac catcggcggt
     2341 ccgcccaact actcggaacc ccgccccttc ttccactttt atggttagtt ctgaaaggat
     2401 taggtagata tcgcaaccca cctcgctcgc ttgcccgagg gtactcgcaa ctgcgaatcc
     2461 agcaatgaag ataaagcgcc tcaatagacc cattcgttat cttttcgaac gtttttccat
     2521 ggagacgtag cgctatttat gatacaaagc ggcaaaatct tccccctagt ttggcttttc
     2581 taaataaggc ggtgtttttc gtgagcggtt taaaacggat tgtgatcact ggtggtccaa
     2641 gcgctggaaa aacaacgctt ttagaccttt tacaaaaaca gtacggcgac cgcgtggcca
     2701 ttgcgcccga agcagccagc atgttgcttc gagggggttt tatgcgccca gacaccgatg
     2761 ccgaccgggt ccacacccag cgtgctattt ttagccttca acgggaactc gaagccaaag
     2821 cgatcgctca ttgcgcgacc acccatggag tttgcgaaac aaaagtttcg ctcttttgtg
     2881 atcgcggctc aatggacggg gcagcctact ggccgggcac cgccgagtcg ttcgtggccg
     2941 aaatgggaac caccgtcgaa cacgaatact cgcgctacag tttggtgcta cacttgcaat
     3001 ccgcggaaga aggcagaggg tacacgcgta ccgaaattcg taccgaatca gcctc
//
LOCUS       DAOMXO010000241         3048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18096_length_3048_cov_4.405947, whole genome shotgun sequence.
ACCESSION   DAOMXO010000241 DAOMXO010000000
VERSION     DAOMXO010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18096_length_3048_cov_4.405947"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLH57_05960"
     CDS             complement(<1..486)
                     /locus_tag="PLH57_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HRK02192.1"
                     /translation="MNESKSNLKTALVFPGQGAQFIGMGKDFFENFKASALVFEEASD
                     ACKLNLKKLCFEGPIEKLTETSSLQPALVATEAAMLAALKSESMLEVSFAAGHSLGEY
                     VALLSSGILTTEAAVRLTAARGAAMQAATPPGTGSMAAILGLNDETTIALCETATRDC
                     ID"
     gene            complement(493..1491)
                     /locus_tag="PLH57_05965"
     CDS             complement(493..1491)
                     /locus_tag="PLH57_05965"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRK02193.1"
                     /translation="MAQRYYTKILGTGSAFPQRSVSNAELAASVDTSDEWIYERTGIR
                     NRRIASLKGDETNSGMAARAALAALEVAKTTPEEIDRIIVATVSPDHVIPNASCALQE
                     RIGAKNAAAFDLSAACSGFVYGLELSNALIQSGMAKKILLVGSEVLSSFVNWKDRGTC
                     ILFGDGAGAVVLGRSTDESSQICSTHLQADGWSKDLFYITAGGSSIPVSHATVDENLQ
                     FMQMKGREIFKVAVKMLADCSERALEKSGYSISDVDWIVPHQANLRIIEALAKRMQVP
                     MQKVILNIEDYGNTSSATVPSAFDLAVREGRIRRGDLVLFTVFGAGLTYGASVLRY"
     gene            complement(1506..1700)
                     /gene="rpmF"
                     /locus_tag="PLH57_05970"
     CDS             complement(1506..1700)
                     /gene="rpmF"
                     /locus_tag="PLH57_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRK02194.1"
                     /translation="MAVPKKKVSPGRKKQRRQFYALKPEGHVVCPNCLDTRRPHRVCV
                     CGFYDGRQVTQPKTQSTETA"
     gene            complement(1754..2347)
                     /locus_tag="PLH57_05975"
     CDS             complement(1754..2347)
                     /locus_tag="PLH57_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRK02195.1"
                     /translation="MKIYFHELSEEPKHLTFTQAEAWLAEAVCQTWETSVEDAVTPSL
                     SPTPYTVDFELRRSQEMIFLKGTLIDVAVELLCSRCATPFFQKISGKFQGIYSRSRDL
                     LEKKKGTIGVAYSEPTGGTEDDLDIEYLEKDSLELADVLKEHLYLKIPFQPLCSDACK
                     GVCPTCGQNQNTDPCQCHRLREGPLAKALAKTKIRVD"
     gene            complement(2427..>3048)
                     /locus_tag="PLH57_05980"
     CDS             complement(2427..>3048)
                     /locus_tag="PLH57_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02196.1"
                     /translation="VSLAELERMAHENSLEIGGLKTLARGSRLYAFGLAQLLQTDSRT
                     SFLLAALLDQKIADSERLRLSTHHTDVSAPIAVLPPVTRILSSDLPTFWELTDKHGLA
                     NVARMALAHFRIRAERILVHRSHLGSIFGETSRAFVRASRELHTPRSLLVRSTVHRGS
                     ATAEQVVDLQRTIARIDHWLRRAEQALAEAGAPTCATMLSGGAPPL"
BASE COUNT          721 a          741 c          831 g          755 t
ORIGIN      
        1 atcaatgcag tcgcgagtcg cagtttcgca taacgcgata gtggtctcgt cgttgagtcc
       61 gagaatggca gccatggagc cggtccccgg aggtgtggcc gcttgcattg cggcgcctcg
      121 ggccgcggtc aagcgaactg cggcttcagt agttaaaata ccagaagata agagtgctac
      181 gtattcaccg agactgtgcc cggccgcaaa agaaacctct agcatgcttt cactttttag
      241 tgcggccaac attgcggctt cggtcgccac caatgcgggt tgcaacgaag aagtttcagt
      301 gagtttttca attggtcctt cgaaacaaag ctttttcaaa ttcaatttac acgcgtcaga
      361 agcttcttcg aaaacaagcg ctgaggcttt gaagttttca aaaaaatctt tacccattcc
      421 aatgaattga gctccttggc ctggaaacac gagtgcggtt ttaaggttcg atttcgattc
      481 gttcataatc gtttaatagc gcagaactga agccccatat gtcagaccag caccaaaaac
      541 tgtgaatagc actaagtcgc cacgtcgaat acgcccttcc cgaacagcca ggtcaaatgc
      601 gcttggcact gttgctgagg aggtgttgcc gtagtcttca atgtttaaaa taactttttg
      661 catgggcact tgcatgcgct ttgctaaagc ctcgattatc cggagattgg cttggtgagg
      721 cacgatccag tcgacgtcag aaatcgagta gccacttttt tcaagggctc gttcgctgca
      781 gtcggccaac atttttacgg cgactttaaa aatttcccgc cccttcattt gcatgaactg
      841 gaggttttca tcgactgtag cgtgagaaac aggaatcgaa cttccaccgg cagtaatata
      901 aaataaatct ttcgaccacc cgtcggcttg taggtgagta gaacaaattt ggctagattc
      961 gtcggtggag cggcccaata caaccgcccc ggcaccatcg ccgaatagaa tacaagtgcc
     1021 ccgatccttc cagttcacaa acgaagagag tacttcgctt cccactagca gaatcttttt
     1081 agccattccg gattgaatca aagcatttga aagttccaaa ccgtatacaa atccggaaca
     1141 ggcggccgac aaatcaaaag cggccgcgtt ttttgctccg attcgctctt gaagtgcgca
     1201 ggaagcattg ggaattacgt ggtcaggaga tacagttgca acaattatac ggtcgatttc
     1261 ttcaggcgta gttttagcaa cttcgagcgc ggcgagagct gctctcgccg ccattccgct
     1321 gtttgtttcg tcgcccttga gggatgcgat gcgccgattt cgaataccgg ttcgttcgta
     1381 tatccattcg tcggatgtgt cgaccgatgc cgctagttca gcgttagaaa cggagcgctg
     1441 cggaaaagcg ctcccggtgc cgagaatctt ggtgtagtaa cgttgcgcca aaacgattgt
     1501 ttcgtttacg ctgtttcagt ggactgagtt tttggctgcg taacttgtcg accgtcatag
     1561 aaaccacaca cgcacacgcg atgtgggcgg cgggtgtcta agcagttcgg gcaaactaca
     1621 tggccctcgg gcttgagtgc ataaaactgc cggcgttgtt ttttgcggcc aggtgaaact
     1681 tttttctttg gaactgccat ttttctctcc tatttggacc tattcgggaa acggacctat
     1741 atcaaaaaaa aaactaatcg acacgaattt tcgttttagc cagcgctttg gctagcggac
     1801 cctctcgcag ccggtggcat tggcagggat cggtattttg gttttgcccg caagtgggac
     1861 aaacaccctt gcaggcgtcg ctacagaggg gctgaaacgg aatctttaag taaaggtgtt
     1921 cttttaggac gtctgcgagc tccagagagt ctttttcgag atattcgata tcaaggtcat
     1981 cttcggtgcc accggtgggt tcgctgtacg ccacgccgat ggtacccttc ttcttttcga
     2041 ggagatctcg agatcgggaa tagatccctt gaaatttgcc actaattttt tgaaaaaagg
     2101 gagttgcgca gcgagagcag agtaattcca cggccacgtc gatcagcgtg ccttttaaga
     2161 agatcatttc ctgagaccgt ctgagctcga agtccacggt atatggggtt ggagacaggc
     2221 tcggcgtcac ggcgtcttcg accgaggttt cccaagtttg gcaaacggcc tcggccaacc
     2281 aggcctcggc ctgagtgaac gttaggtgtt tgggttcttc cgaaagttcg tgaaagtaaa
     2341 tcttcattat ttaagccaaa agggcttaaa aaacatatcc atacgtcaaa gtcaagaatt
     2401 ccatgccggc acgaactggc gtcgacttaa aggggaggcg cgccaccgct cagcatagtg
     2461 gcgcaggttg gtgctccggc ctccgcgagg gcctgttctg ctcttcgaag ccagtggtcg
     2521 atgcgggcga tcgtgcgttg gaggtcgacg acttgttcag cggtcgcgga accgcgatgc
     2581 acggtcgacc gtacgagaag gcttcgagga gtgtgcagtt cgcggctcgc tcgcacgaat
     2641 gcgcggctgg tttcgccgaa gatggaaccc aagtgactgc ggtgaactaa aatgcgctcc
     2701 gcccgaattc gaaagtgagc gagcgccatg cgcgccacat tggctagacc gtgtttgtcg
     2761 gtgagttccc agaaggtggg aaggtcggac gaaaggattc gtgtaactgg cggcaaaaca
     2821 gcgattgggg cggaaacgtc agtatggtga gtggaaagtc gcaagcgttc ggagtcagca
     2881 attttttgat ccagtagtgc ggcaagtaga aaagaggtgc gggaatctgt ttgcaacagc
     2941 tgggctaggc cgaaggcata gaggcggctc cctcgggcca gtgttttcaa cccgccaatt
     3001 tcgagcgaat tttcgtgagc catgcgctct agttctgcca gggagacg
//
LOCUS       DAOMXO010000242         3047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18102_length_3047_cov_4.975936, whole genome shotgun sequence.
ACCESSION   DAOMXO010000242 DAOMXO010000000
VERSION     DAOMXO010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18102_length_3047_cov_4.975936"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PLH57_05985"
     CDS             complement(<1..1246)
                     /locus_tag="PLH57_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK02197.1"
                     /translation="MFFLALRHLISRKRQTTLTLLGITLGAAAYIAISAMMLGFQEFI
                     IDQLVNNDSHVRINAREEMLNEQVLNDSFFPDGELVNWVKPPMGRKDNAYILAPRAWL
                     DRLEADEKVQAASPQLVAQAIANYAKLTVGVRLIGSIPDIQRRVSNIEKYMLKGKFSD
                     IGASGNRIVVGKEFLDKIGASIGESILLTVGKSAPQPFKIVGVFRLGVKSLDETTVFG
                     ALSDVQKLNQTPSRISDIAIRLVDVEQASELASNLNLMGQEKVQSWNQANEGIMSVFK
                     TQDIVRNSMTFAILIVAGFGIYNILSLAVSHKRREIAILRSMGFEPRDISELFLTQGV
                     ILGVIGGLLGVVIGLGASYGMSLVRVSEARGLGTGQMMVSFDGLIYVKAFLLACIASS
                     VASFFSARAAGRLEPIDIIRGEN"
     gene            complement(1262..2026)
                     /locus_tag="PLH57_05990"
     CDS             complement(1262..2026)
                     /locus_tag="PLH57_05990"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRK02198.1"
                     /translation="MNKKWLLVFLLVALVGATSWVFFKKVQPRERVKAKRGTVVESIY
                     GLGTVVADHDFHLRTGVPVSVTKLFVSEGDKVEKGSPLVRIDENTMVSPIVGAVSTVA
                     YKEGELAPAHAVIVSVMNLSKLHLEVSLEQQSVLRVKQGQNVVVSFESLRNERTEGIV
                     KAIYPRENQFIVRIDLNSWPEGVLPGMTADTAILVGRKSDVILIPVRAIKAGLVTRVR
                     NGKKEKLPVKLGVLDGEWAEVLSENVAESDELVVQK"
     gene            complement(2059..2607)
                     /locus_tag="PLH57_05995"
     CDS             complement(2059..2607)
                     /locus_tag="PLH57_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02199.1"
                     /translation="MKKLRPYILRFQGYADRFWYPPLVGVLSLLDNFVLIIPNDGILI
                     SSSMMIPKRWLALALSVALGSTFGALILAALVEYQGLPWILNLYPGIGETKVWAWSFK
                     VFDAYGLFIVFVIALTPLMQQPAVILASLANTPLPKLAAAIFAGRFLKYLFLAYLGSH
                     APEFLGKLWGLKGELRDTGVLK"
     gene            complement(2620..>3047)
                     /locus_tag="PLH57_06000"
     CDS             complement(2620..>3047)
                     /locus_tag="PLH57_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02200.1"
                     /translation="VVPTTTTFNGSRFFVIENGVRKATPLFVALIIIEVTDLVFAVDS
                     IPAVFAVTQDAFVAFASNILAILGLRALYFVLSDLVVKLRYLKPGLAAILGFIGVKML
                     LIDFFKIPSWVSLLVIFSILTTAGLSSWYVARLETRTSK"
BASE COUNT          859 a          820 c          649 g          719 t
ORIGIN      
        1 agttctcacc tcggataata tcgatcggtt caagtcttcc tgccgcccgg gctgaaaaaa
       61 aactcgcaac actcgatgca atacaagcga gcaaaaaggc cttcacataa ataagcccgt
      121 caaacgagac catcatttgg ccagtgccca agccccgcgc ctcagacacc cgtacgagcg
      181 acattccgta ggatgcgccc aatccaatca ccacgccaag caaaccacca attactccca
      241 gaatcacacc ctgagttaaa aacaactcgc tgatgtcgcg aggctcaaac cccatcgatc
      301 ttaaaatggc tatctcgcgg cgcttgtgac tcacggctaa acttaaaatg ttataaattc
      361 cgaatcccgc gactattaag atggcgaaag tcatcgagtt tcttacgata tcctgagttt
      421 tgaataccga catgatcccc tcgttggcct ggttccaact ctgaaccttt tcctgtccca
      481 tgagatttaa attagaagca agctcgctag cctgttctac gtccaccaag cgaatcgcaa
      541 tgtcggaaat tcttgaaggg gtttgattta acttttgcac gtcactcaac gcgccaaaaa
      601 ccgtggtttc gtcgagtgat ttcacgccca ggcgaaacac gcccacgatc ttaaacggct
      661 ggggagcaga tttccctacg gttaatagaa tgctctcgcc aatcgacgca ccaatcttat
      721 cgagaaactc tttgcctaca acaatgcgat tgccacttgc accaatgtcc gaaaatttac
      781 ccttgagcat gtatttttca atattggaca ctcggcgttg aatatcggga atagaaccaa
      841 tcagtcgcac gccgacggtc agtttcgcat aatttgcaat tgcttgagcc accaattgtg
      901 gcgaggcggc ctgcactttt tcgtcggctt caagtcgatc aagccaggcc cggggtgcca
      961 aaatataagc gttgtccttg cgccccatcg gaggtttcac ccaatttacc agttccccgt
     1021 cgggaaaaaa tgagtcattt aatacttgtt cgttgagcat ttcttcacgg gcgttaatgc
     1081 gaacgtggga atcgttgttc accaactggt caatgataaa ctcttgaaat cccagcatca
     1141 tggctgaaat ggctatgtaa gcggcggcac ccaaggtaat tccgagaagc gtaagagtgg
     1201 tttgcctttt tcgactaatt aaatgtcgaa gcgctaagaa aaacaattca gcttcctctt
     1261 cctatttttg tacgactaac tcgtcggatt ccgccacatt ctctgaaagg acttccgccc
     1321 attccccgtc gagcacaccg agttttactg gcagtttttc tttcttacca ttgcgaacgc
     1381 gtgttaccag accggcttta atggcacgca caggaattaa aattacatca gacttccgac
     1441 cgacgagaat tgcggtgtcg gcggtcattc ccggcaaaac accttcgggc cacgaattta
     1501 aatctattct tactatgaat tgattctcac gcggataaat tgctttaacg atgccctcgg
     1561 ttcgttcgtt tctcaaactt tcgaacgaca ccactacgtt ttgcccttgt ttcacccgca
     1621 acacggattg ctgttcaaga ctaacttcta ggtgcagttt ggacaggttc atgacactca
     1681 cgatcacggc gtgggcaggc gcgagttccc cctctttgta ggccaccgtg ctcaccgcac
     1741 cgacaatcgg ggaaaccatt gtgttctcgt ctattcgaac gagtgggctc cccttttcga
     1801 ccttatcgcc ctcactaaca aaaagttttg tcaccgaaac cggcacgcca gtgcgaaggt
     1861 gaaagtcgtg gtccgccacc acggttccga gtccgtaaat actctcgacc acagtgcctc
     1921 gtttggcctt tactcgctca cgaggttgta cttttttaaa aaacacccac gacgtagcac
     1981 caacgagcgc caccagcaaa aacaccaata gccacttttt attcatgtac ttagttttag
     2041 cgggctcggg ctagcgtgtc acttcaaaac cccggtatca cgcaactcac ccttgaggcc
     2101 ccacaactta cccaaaaact cgggtgcgtg agaacctaag taggccaaaa acaaatactt
     2161 taaaaaacgc ccagcaaaga tggcggcggc taactttggt agcggggtat tcgcgagact
     2221 tgcgagaatc actgcgggtt gctgcatgag cggcgttaac gcgatcacga aaacgatgaa
     2281 cagcccgtag gcgtcaaaaa ccttaaacga ccaggcccac accttggttt cgccgattcc
     2341 ggggtaaaga tttagaatcc agggtagccc ttggtactcg accaacgcag ccaagatcag
     2401 cgccccgaaa gtggacccga gtgccacact caaagctagc gccaaccagc gtttgggaat
     2461 catcatggag ctggaaatta aaattccgtc gttaggaata attaaaacaa aattgtccaa
     2521 caaagacagt acgcccacga gcggagggta ccaaaaacgg tccgcgtaac cctgaaaccg
     2581 cagaatgtag ggccttaatt ttttcaagtt cgcaattccc tatttcgaag ttcgagtctc
     2641 tagtcttgca acataccaag aactaagacc tgccgtcgtg agaatggaaa aaataaccaa
     2701 gagtgaaacc cagctgggaa ttttaaaaaa atctatcagc aacatcttaa cgccgatgaa
     2761 ccctaaaatc gctgccaaac ctggttttag ataacgcagt ttcacgacca ggtcagagag
     2821 tacaaaataa agagcacgta gtccaagaat agctaatata ttcgaagcaa acgccacaaa
     2881 agcgtcctgg gtaacagcga aaacagcggg aatcgaatca acagcgaata ccagatctgt
     2941 cacttcgata atgattaacg ctacaaacaa cggagttgct ttacgaacac cgttttctat
     3001 cacaaaaaag cgactaccat taaaagtggt ggtcgtcggc acgactc
//
LOCUS       DAOMXO010000243         3045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18116_length_3045_cov_4.504682, whole genome shotgun sequence.
ACCESSION   DAOMXO010000243 DAOMXO010000000
VERSION     DAOMXO010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18116_length_3045_cov_4.504682"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2000)
                     /locus_tag="PLH57_06005"
     CDS             complement(<1..2000)
                     /locus_tag="PLH57_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02201.1"
                     /translation="MGLLNVLRTIALAIGLVVGFSGIRNIAAATKRVAPTCAEILAER
                     ALEAISSTRLTPNEAWSKLKKDLELASGPTVLLTSFSISANVSMAMLMELGKLAASLG
                     VEVDFELSTANAVRTKTSSIIYQNFVFSGPAAKIAKLMTSIASKPFVETLTLRHSEVL
                     NALVDEPLILPALKTLDSTSEKNQNELYKVAFEKAAQAPVLLIDQPGRFSVLVASEIF
                     DRWAKNSKVASAFKVHRTTLSDMAKSLNGLGAIDRHSKRGVYQVSDPTSKNQIYVISM
                     DVFSILQREAADGVLGESEPAVLREKIRSLLKVETQQIAPILEDLPIEAPAKPSIAEL
                     KSAKTKAERRTETISGKQSLSISAFIFLNNKQNLLISPAGVVIEKQGSGPNGVQRNRA
                     TIPRAVYFAARPELDAQYVVEQIQKHLERYDASLVLSHDQVEQLREPIARLLSDPAPS
                     DIQLIPNDPNGRLRRLNELTLGTERPPVLLKRASSGIYGLLIPIAPVPEVAKSTATAE
                     SIEPGPVTQAPMTSDRTHLGSPTQDLVGQMELIRPGMEISEIGPKTILGRFKPFREFL
                     NRPETYLDGLMGDPKTSTAGAPFLVLTRRSVNSTAESPYLVLFSESYFGAATEELLFA
                     QYRYALQLGEGESLTGGLQRRFLDELLGERTRNAEVMTAKALR"
     gene            complement(2092..>3045)
                     /locus_tag="PLH57_06010"
     CDS             complement(2092..>3045)
                     /locus_tag="PLH57_06010"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HRK02202.1"
                     /translation="GCSFCAITLHQGRIVQSRSQDSVLKELGDMKNVPGFKGTVSDIG
                     GPTANMYQLRCKSTEIQSKCRKLSCVHPKVCPHLHTDHTPQVELLEKARNIPGIKKIR
                     ISSGLRYDLALHDQKSGRKYLRDLISHHVGGHLKVAPEHLDEDVLHLMKKPTMSNFDE
                     FREIFEKVSKDAGKEQYLIPYFISGFPGTTHEQMEKVYDYMKERGWRLQQVQAFIPTP
                     MTLATAMFWTGIDPMSKKELFVPRDWNDRKIQQALLQPHKPEHRDILRKYRQQRRLAA
                     NKPSAAPHPSNRTKLVPRSSKRSLVEPPKSKMGPHLYLDER"
BASE COUNT          750 a          734 c          743 g          818 t
ORIGIN      
        1 cggagggcct tagctgtcat aacctcggca ttacgagttc gttcccccaa gagttcatct
       61 aaaaaccgac gttgcaatcc accggtcagc gattccccct cgcccaactg caaggcgtag
      121 cggtactgtg cgaaaagtaa ttcttcggtg gctgcgccaa aatagctctc actaaataat
      181 acaagatagg ggctttcagc cgtgctattc acactacggc gcgtgagtac caaaaacggc
      241 gcaccagcgg tgcttgtttt aggatctccc atcaagccgt cgaggtaggt ttcaggacga
      301 ttcagaaatt cgcgaaatgg tttaaagcgc cccaagattg ttttgggccc aatctctgat
      361 atctccatac cggggcgaat caattccatt tgcccaacga ggtcctgcgt tggagaacct
      421 aaatgcgttc gatcgctggt catgggcgct tgagttaccg gcccgggttc gatcgattcg
      481 gcagttgccg ttgattttgc gacttctgga actggtgcga taggaatgag aagtccgtag
      541 attcccgacg aagcgcgctt taaaagtacc ggcgggcgct ctgtgcccaa tgtcagttca
      601 ttcaaacgac gcaaacggcc attcgggtcg tttggaatca gttgaatatc tgatggagcg
      661 ggatccgaca gcaaccgcgc gataggctcg cggagttgct ccacttggtc atgagaaagc
      721 actagcgacg catcgtagcg ctcaaggtgc ttttgaatct gttcgactac atattgcgcg
      781 tccaattccg gccgggccgc aaaatatacc gctctcggaa tggtcgcgcg gtttctttgt
      841 actccgttgg gaccggaacc ttgtttctcg atcacgacgc ctgctggtga aatcagaaga
      901 ttctgtttat tgtttaaaaa tataaatgca ctaatgctga gagattgctt gcccgagatc
      961 gtttcagtgc gtcgttcggc tttagtttta gcgcttttta attctgcgat cgaaggtttt
     1021 gctggggctt cgatgggaag atcttctagt ataggagcga tctgctgcgt ttcgactttc
     1081 aggagacttc taattttctc tctaagaact gcgggctcac tttctcctaa cacaccatcg
     1141 gctgcttcgc gctgaaggat cgaaaataca tccatcgaaa ttacgtagat ctgattctta
     1201 cttgtaggat ccgaaacttg atacacccca cgttttgagt gtcgatcaat ggcgcccaac
     1261 ccatttaagc tcttagccat atcggataag gtagttctat gaactttgaa agcactagcg
     1321 actttggaat tcttagccca acgatcaaaa atttcgctgg caaccaaaac agaaaaacgg
     1381 ccgggctgat caattaaaag gaccggggct tgtgcagctt tttcgaatgc cactttatac
     1441 agttcattct ggtttttctc gctagtacta tcgagtgttt ttaacgccgg aagaatcaac
     1501 ggctcgtcaa ccaaagcgtt gagcacttca ctgtgacgta gcgttaaagt ctcaacaaag
     1561 ggcttcgacg caatcgaagt catcaatttt gcgatcttgg ccgcaggtcc ggaaaaaacg
     1621 aaattttggt aaataattga acttgttttg gtgcgcaccg cattggctgt agaaagttca
     1681 aaatcaactt caacgcccaa cgaagcggca agttttccta attccatcaa catggccatt
     1741 gagacatttg cagaaataga aaaactggtg agcaaaaccg ttggaccgct ggcaagctcg
     1801 aggtccttct taagtttgct ccaggcttcg ttcggagtca gacgagtact ggatatagcc
     1861 tctagcgcac gttctgcgag aatctcagca caagtggggg cgacccgttt tgttgccgcc
     1921 gcgatatttc gaatcccact aaatccaaca accaatccga ttgcgagggc aattgttcga
     1981 agaacgttga gtaaacccat gattcacctc aaaaataaag tggagcttta ttatcaagat
     2041 ttggtaccaa tctcaatatc taaataatgc ggcacgttat tttgaaccaa actatcgttc
     2101 gtctaggtac agatgaggtc ccattttaga tttgggtggt tccaccagag aacgtttaga
     2161 agagcgagga accagcttgg ttcgatttga cggatgcggt gccgcggacg gcttatttgc
     2221 agccagacgg cgttgctgac ggtacttacg caggatatca cggtgctctg gtttatgagg
     2281 ctgcagtaga gcttgttgaa tcttgcgatc attccagtcg cgtggaacga agagttcctt
     2341 tttactcatg gggtctatgc cggtccaaaa cattgcggta gcgagggtca tcggggttgg
     2401 aatgaacgcc tgaacttgtt gcaaacgcca accccgttct ttcatgtagt cgtacacctt
     2461 ttccatttgc tcatgcgtgg tgcccggaaa accgcttatg aaatacggga tcaagtactg
     2521 ctctttgccg gcgtctttcg aaaccttctc gaaaatttcc cgaaactcat cgaaattgga
     2581 catggtcggc tttttcatta aatgcaagac gtcttcgtcg aggtgttcgg gtgctacctt
     2641 caagtgaccg cccacgtggt gtgaaattaa atctcttaaa tatttgcggc ccgatttttg
     2701 atcatgcaac gcgagatcgt agcgcaagcc agacgaaatg cggatttttt taatgcctgg
     2761 aatgttacgg gctttttcca acagctccac ctgcggcgtg tggtcggtgt gcaggtgcgg
     2821 acaaactttc ggatgtacac acgaaagttt tcggcactta ctttgaatct cagtactctt
     2881 gcaacgcaac tggtacatgt tggccgtcgg tccaccaata tcactcactg tgcccttgaa
     2941 tcccggcacg tttttcatgt caccgagttc ttttaaaacg ctgtcttggg aacgcgactg
     3001 cacgatgcgc ccctggtgca acgtaatcgc gcaaaaagaa catcc
//
LOCUS       DAOMXO010000244         3043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18138_length_3043_cov_3.963186, whole genome shotgun sequence.
ACCESSION   DAOMXO010000244 DAOMXO010000000
VERSION     DAOMXO010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18138_length_3043_cov_3.963186"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2564)
                     /gene="carB"
                     /locus_tag="PLH57_06015"
     CDS             complement(<1..2564)
                     /gene="carB"
                     /locus_tag="PLH57_06015"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRK02203.1"
                     /translation="MPKNTTIHSILLIGSGPIQIGQACEFDYSGTQAARALKAEGYRV
                     ILVNSNPATIMTDPEMADTIYIEPLTREFLETIIRKEKPDAILPTMGGQTALNLACEL
                     AESGFLDEQGVRMLGANLESIKKAEDRDLFKKTMIELGIDVPKSRLIRSISDGRACLQ
                     EIGLPMILRPSFTLGGEGGGIVHTEEEFLKTLERGLFLSPVRSVLVEESLVGWKEYEL
                     EVVRDSKDNVIIVCSIENVDPMGIHTGDSITVAPAQTLTDRQYQHLRDLSKKIIRAIG
                     VETGGSNIQFAVKPWGSDAGRVIVIEMNPRVSRSSALASKATGFPIAKVAARLAVGYT
                     LDELKNEITGTTPASFEPTIDYVVTKIPRFDFEKFTSSPPLLGTQMKSVGEVMAMGRT
                     FKESFFKALASLENSRTWLSTTELDDKNTSIAEIEDELKKPHALRIWYVGSAIRRGLT
                     TKRIHELTAIDPWFLNQIRDVLDFFDRHEKNKKPIREWTDAEILEAKQRGILDREIAR
                     LCEGADEWAVRIERTKRKIHPSYPLVDTCAAEFKAQTPYLYSSYNTRDSENPLTEQSV
                     MILGGGPNRIGQGIEFDYCCVHASVAVREARHRSVMVNCNPETVSTDYDISSRLFFEP
                     LTAEHVIEIARTLPNLRGAILQFGGQTPLKLAKALQRAGIPILGTAAENIDIAEDRER
                     FEALIKELAPLGLKQPPSRTARTREEALKAAEEIGFPLLLRPSYVLGGRGMRMIYSSD
                     ALAQFIDEAILISDQHPVLLDRFLVNAIEVDVDAIGDGADIVVGGIMEHVEEAGIHSG
                     DSACSLPPFTLGPETCTRLREYTRLLAEKLKIVGLMNVQFAVQDSDIYVIEVNPRA"
     gene            complement(2567..>3043)
                     /locus_tag="PLH57_06020"
     CDS             complement(2567..>3043)
                     /locus_tag="PLH57_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02204.1"
                     /translation="KLFEPDGGCVRHYTYKNKTYPADSSRKPDGEGLRKLFKKSPQAE
                     EMLNNYQSRLTTLRWPAITGSIGVASMITGVIYASQLDTQLGRRDTRLVMIGAGASLI
                     LASYAYGQLGRHLNEKNLEKAVETYNDSVPKEERIQVSITPTENVSGGMIQTLVPF"
BASE COUNT          712 a          734 c          737 g          860 t
ORIGIN      
        1 cttgctcggg gattaacctc aataacgtaa atatcactgt cctgaaccgc aaactgcacg
       61 ttcatgagtc ctacgatttt gagcttttct gccaggagcc gggtgtattc tcgcaagcgt
      121 gtgcaagttt cagggcctag agtaaaagga ggaaggctgc aggcactgtc gccggagtgt
      181 atgcctgctt cttcgacgtg ttccatgatt ccgccgacaa ctatgtcggc tccgtctcca
      241 atagcgtcta catctacttc aattgcattt actagaaatc gatcaagtag cacggggtgt
      301 tgatcggaaa tgagaatggc ctcgtcgata aattgcgcga gagcgtcgct cgaatatatc
      361 atgcgcattc cgcgtccacc caaaacataa ctggggcgca acaaaagcgg aaatccaatt
      421 tcttccgcgg ctttgagtgc ctcttctcta gtccgagcgg tgcgactggg cggttgtttt
      481 aaaccgagag gggcaagctc tttgataagt gcctcgaatc gttcgcgatc ttcagcaata
      541 tcaatatttt ctgctgcggt tcctaaaatt ggaatgccgg cgcgctgaag tgccttggca
      601 agtttcaatg gagtttgacc accaaactgc aatatcgcac ctcgaaggtt cggaagcgtg
      661 cgcgcaatct caattacgtg ttctgcggtg aggggttcga aaaaaagccg tgaagaaata
      721 tcgtaatcag tacttacggt ttctggattg caattgacca tgacactgcg atgtctagct
      781 tcgcgaacgg ccacagaagc atgtacgcaa caatagtcga attcaatacc ctgtccgatc
      841 cgatttgggc ctccgcccaa tatcatcacg ctctgttcgg tgagaggatt ttctgaatca
      901 cgggtgttat agctgctata caagtaagga gtctgagctt taaattccgc ggcacaggta
      961 tccaccaggg gataacttgg gtgaatcttc cgtttggtac gttcgattcg tactgcccat
     1021 tcgtcagcgc cttcgcaaag acgggcaatt tcacgatcaa gaattcctcg ttgttttgct
     1081 tctaagattt cagcgtcggt ccattcgcgt attggctttt tatttttttc atggcgatcg
     1141 aaaaaatcta gaacatctcg aatctgattt aaaaaccaag gatcaatcgc cgtaagttcg
     1201 tgaattcgtt tagtagtgag tccccggcga atggcgctgc ccacatacca aatgcgcaat
     1261 gcatgaggtt tttttagttc gtcctcaatt tcggcgattg aagtgttttt atcgtctaac
     1321 tcggtagtgc ttaaccaagt gcgggagttt tcgaggctag caagagcttt gaaaaaggat
     1381 tccttaaatg ttcggcccat agccattacc tcgccgacgc tcttcatttg agtgccaagc
     1441 aatggcgggc tagatgtaaa tttttcgaaa tcaaagcgtg ggattttagt aacgacgtaa
     1501 tcgatcgtag gttcgaaact tgctggagtg gtacccgtta tttcgttttt aagttcatcg
     1561 agcgtataac cgacggccaa tcgcgccgcc acttttgcaa taggaaaacc ggtcgccttg
     1621 ctcgccagtg ccgacgatcg cgaaactcga ggattcattt caataacaat tacgcgaccg
     1681 gcatcggatc cccagggttt tacggcgaat tgaatgttgg agccgccagt ttccactccg
     1741 attgcgcgaa ttattttttt cgataaatct cgaaggtgtt ggtactgtcg atcggtcagc
     1801 gtctgagcgg gagctacagt aatgctatca ccggtgtgaa ttcccattgg atcgacgttt
     1861 tcgatcgagc agacaattat tacgttgtct ttagagtccc gcactacctc tagttcgtat
     1921 tctttccagc ccactaagct ttcttcgact agcacagagc gcaccgggct taagaataag
     1981 ccacgttcca gagtttttaa aaactcctct tcggtgtgaa cgatgccacc gccttcgccg
     2041 cctagcgtga agctcggtcg aagaatcatc ggcagtccga tttcttgtaa acaagctcgt
     2101 ccgtctgaaa tagatcttat caggcgactt ttcggcacat cgattcccaa ttcgatcatc
     2161 gtttttttga agagatcgcg gtcttccgct ttttttattg attcaaggtt ggcgcccaac
     2221 attcgaacgc cctgctcatc cagaaaacct gactcagcta attcgcaggc tagattcagt
     2281 gctgtttggc cccccatggt gggcaaaatg gcatcgggtt tttcttttcg gataatagtt
     2341 tccaaaaatt ccctggtaag aggttcgatg taaattgtat cggccatttc tggatcggtc
     2401 atgatcgttg cgggattgga gtttactaaa atgacacgat aaccctcggc tttcagcgcg
     2461 cgcgctgctt gggtgccaga gtagtcaaac tcgcaggcct gcccaatctg aattggcccg
     2521 gagccgatca ataagatact gtgaattgtt gtattttttg gcattgctag aacggaacca
     2581 aggtttggat cattcccccc gagacatttt cggtcggagt gatgctcact tgaatacgtt
     2641 cctccttcgg gaccgaatca ttataggtct caacagcttt ttccaggttt ttttcgttta
     2701 aatgccttcc aagttgtccg taggcgtaac tcgccaatat caaactcgct cccgccccga
     2761 tcatgaccag tcgtgtgtcg cgacgtccta actgggtgtc tagttgcgat gcgtaaataa
     2821 cgcccgtaat catagaggcg acgccgatgc tccctgtgat ggcgggccag cgaagggtgg
     2881 ttaaacggga ctggtagtta tttagcattt cctctgcctg cggagatttt ttaaataatt
     2941 tgcgcagtcc ctctccgtcg ggttttctac tcgagtcggc agggtaggtc ttatttttgt
     3001 aggtatagtg ccgtacacaa ccgccgtctg gctcgaaaag ctt
//
LOCUS       DAOMXO010000245         3041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18144_length_3041_cov_5.986269, whole genome shotgun sequence.
ACCESSION   DAOMXO010000245 DAOMXO010000000
VERSION     DAOMXO010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18144_length_3041_cov_5.986269"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLH57_06025"
     CDS             <1..290
                     /locus_tag="PLH57_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRK02205.1"
                     /translation="SSLTPPDLLIYLRKSLPKLRKNIQTRGRGYEEKISDGYLSNLNR
                     YYDEWIGSYSDGKVLVIDSDDLDFVKYNEDFEGICRRIESQFDQQELFLFS"
     gene            358..1173
                     /locus_tag="PLH57_06030"
     CDS             358..1173
                     /locus_tag="PLH57_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRK02206.1"
                     /translation="MLTYSALMKRLLVVAVVASSVGLGGCGGSAEDEDAEWVAENAGA
                     DTAVVESEGDAEVVAAEPVTETVTESKAEIPADSYDAPSQMSTNYGTGEVNHYTVKKN
                     DTLMKIAFEVYGDITRWRDIFDMNRDKLKNANMIMAGTKIKYEKPVQEPDVGHNGEPY
                     LIKSGDTLGSIADDVYAQRSKWKKIYENNKDLIRDPNRIYAGFYIYYQITEEERRRVD
                     EIKGRRNQRLGGMPSAETFGPTTVGSVGGGLNDLAAPDDGEVVEQDRVPTATQ"
     gene            1219..2079
                     /locus_tag="PLH57_06035"
     CDS             1219..2079
                     /locus_tag="PLH57_06035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRK02207.1"
                     /translation="MKRFLLLVFLLVWSGCSTAPNKVDQGVLSPEMESSFEARMYKNL
                     QHQYGLLESKTKLKTDFLKIEKFIQNLVNTLAVSASAPSSSTIKVKIIDSRRLIHGAG
                     LNGSIYLSTGLLKAIKFENELAFVLATQIALLEARHPQISFETIQGRALAESKVYLPM
                     GLSADAGTSEALPTVDGKWFEKGGFFDFGNAAYYQADRSAIQRLVEHRYDPRGGISYL
                     SRLSESNRNFAREPVGQILPPLSERIDELRNEVAKQSPLRNPIVKSAGFELFLRIVNQ
                     TDATGKNKNS"
     gene            2111..>3041
                     /locus_tag="PLH57_06040"
     CDS             2111..>3041
                     /locus_tag="PLH57_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRK02208.1"
                     /translation="MSRLGRSVTVEALPGDASTRRYYRVRTRTASFILMKMQPFEDSG
                     NDLTFIQVQKHLSKLGINVPQIVDVDDKSGVILLSDLGDETLLKRLERVSKKRDQLVW
                     FKRAIDLVLNMQVVATQRSAPIDAYGLYFDEEKLMWEVRFALAHFYGGTLARKIPNKE
                     ERRVQNYFESICKQLASQEWYFTHRDYHSRNIMVVDQDLYMIDFQDARMGCAQYDLAS
                     LLRDSYYQLEEEVVYELVDYYIRQRDKMEGTKTGRKEFVRIFDLMAIQRNFKAIGSFA
                     SFYAKRGDARYLKFIGNTFENIRRNLLRFPELDE"
BASE COUNT          888 a          632 c          746 g          775 t
ORIGIN      
        1 gttcaagcct cactccaccg gaccttttaa tctatttgcg caaatcgtta ccgaaactta
       61 ggaaaaacat acagacccga ggtcgggggt atgaggaaaa aatttccgat ggctacctat
      121 ctaatttgaa caggtactac gacgaatgga ttggtagtta cagcgacggt aaagtgctag
      181 tgatcgactc cgacgacctc gattttgtta agtataacga ggacttcgaa ggcatttgcc
      241 ggagaattga aagtcagttc gaccagcaag agttattcct cttttcataa gtatattaaa
      301 aaaaactgaa gttatttttt ggggcgccga caagtggggt gaaggagaca cccgtatatg
      361 ctcacttatt cggcgttaat gaagaggttg ctggtagtag cagtggttgc cagctcagtt
      421 ggtcttggag gttgcggcgg atcggctgaa gatgaagatg ccgaatgggt cgcagaaaat
      481 gccggagcag acacggctgt agttgaaagc gaaggcgatg ccgaagttgt cgcagccgaa
      541 ccagtgactg aaactgtcac cgagtcgaaa gccgagattc cagcagatag ttatgacgcc
      601 ccatctcaaa tgtcgaccaa ttacggcacc ggggaagtta accactatac ggtaaaaaag
      661 aacgacactt tgatgaaaat tgcgtttgaa gtttacggcg acattacgcg ttggcgcgac
      721 attttcgata tgaatcggga taagttgaaa aacgcaaaca tgataatggc cggcaccaaa
      781 attaaatatg aaaaaccagt tcaggaaccc gacgttggcc acaacggcga accatactta
      841 attaaatccg gtgacacgct cggctcgatt gccgacgatg tgtatgccca gcgttcgaaa
      901 tggaagaaga tttacgaaaa caataaggac ctaatcagag atcctaatcg catctacgcg
      961 ggcttctata tttattatca aattacagaa gaagagcgtc gtcgagtaga cgaaatcaag
     1021 gggcggcgca atcaacgtct tggcggcatg ccctcagcgg aaacctttgg tcctactacg
     1081 gttggcagcg ttggcggcgg acttaacgac ttagccgcgc cagatgatgg cgaagtggtc
     1141 gagcaagatc gagtccctac agccactcaa taaagacgat atctagtcat cacaacagtc
     1201 ttttgttaca gtagtaggat gaaaagattc ctactacttg tgtttctctt ggtctggtcg
     1261 ggctgcagca ccgcgccaaa taaggtcgat caaggggtgc tttctcccga gatggaatcg
     1321 agctttgaag cgcggatgta taaaaatcta caacaccaat atggtttgtt agaatcaaaa
     1381 accaaactca aaactgactt tctgaagatt gaaaagttta ttcaaaattt agttaacacg
     1441 cttgcggtgt ccgcatcagc gcccagttcc agtaccatta aggtaaaaat catcgatagt
     1501 agacgtttaa ttcacggagc gggtttaaat ggcagtatct acttatcaac cggcctgctt
     1561 aaggcgataa agtttgaaaa tgaactcgct tttgtgttgg ctacccaaat cgcattatta
     1621 gaggcccgcc acccccagat cagtttcgaa accattcagg gtcgagcgct ggccgaaagt
     1681 aaggtctacc tcccgatggg gttaagtgca gatgctggaa ctagtgaggc cttacccacg
     1741 gttgatggta agtggtttga aaaaggtggt ttcttcgatt ttggaaatgc cgcttattat
     1801 caggctgacc gaagtgccat tcaacgcctc gttgaacacc gttacgaccc gaggggcggt
     1861 atttcttatt tgagtcgtct aagcgaatca aatcgtaatt tcgcgaggga accggttggg
     1921 cagatattgc cacctctaag tgagagaatt gacgaacttc gcaatgaagt tgcgaaacag
     1981 tcgccgcttc gtaatcctat agttaagagt gcaggatttg agttattttt aagaattgta
     2041 aatcaaacag atgcgaccgg aaaaaacaaa aacagctgaa gttaattacg atgttttggt
     2101 aaaggcttgg gtgtcacgcc ttggccgcag cgtaaccgtt gaagccctgc caggcgacgc
     2161 ctctactcga agatactatc gagtaagaac tcgcactgcg tccttcattt tgatgaagat
     2221 gcagcccttc gaagattccg ggaatgatct tacttttata caagtacaaa aacatctgag
     2281 taaacttggt attaatgttc cccaaattgt cgacgtagat gacaagagtg gcgtgatctt
     2341 gctttccgac ttgggagacg aaactctttt gaaaagactg gagcgagtct cgaaaaaaag
     2401 agatcagttg gtttggttta aacgggcaat cgacttagtt ttgaatatgc aggttgtggc
     2461 tactcaacgt tctgcgccca tagatgcata tggactctac ttcgacgaag aaaaattgat
     2521 gtgggaagtg cggtttgcgc ttgcacactt ttacggcggc acgttagcac gaaagattcc
     2581 aaacaaagaa gagcgtcgag ttcaaaatta ttttgaaagc atttgcaaac aacttgccag
     2641 tcaagagtgg tattttactc accgcgatta ccacagtcga aatatcatgg tcgtggatca
     2701 agacctatac atgatcgatt ttcaagatgc gcgcatgggt tgcgcccaat acgacctcgc
     2761 gtcgcttttg cgtgattcct attatcaatt agaagaagaa gtagtttacg aactggtcga
     2821 ttactatatt cgccaacgag ataaaatgga gggtacgaaa accggacgaa aggaattcgt
     2881 acgaattttc gatctaatgg cgattcagcg aaattttaag gccatcggtt cgtttgcttc
     2941 cttttatgcc aagcgtggtg atgcgagata cttaaaattt attggcaaca cgtttgaaaa
     3001 tattcgtaga aatttgctac gtttcccaga gctcgacgag c
//
LOCUS       DAOMXO010000246         3041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18149_length_3041_cov_3.989953, whole genome shotgun sequence.
ACCESSION   DAOMXO010000246 DAOMXO010000000
VERSION     DAOMXO010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18149_length_3041_cov_3.989953"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLH57_06045"
     CDS             <1..560
                     /locus_tag="PLH57_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02209.1"
                     /translation="AKKYERFKRKFKHYTEDFRITRDPQAADRLTSLHFATSKFHSET
                     IHLLLSLAKKTVAANEFGEVALSQFFDFVAKNEARASDDALLIGSEITRPLYLHIIDR
                     VEELGPEGLQTIAETLFHTQRALSTIASADSERSAKILLLRDTVRPLLEHWDQAGEAE
                     FDKRSYYFNRLELLADADCNSIMAN"
     gene            658..1605
                     /locus_tag="PLH57_06050"
     CDS             658..1605
                     /locus_tag="PLH57_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02210.1"
                     /translation="MNLKLSALCLLSALLGNLALAQQATPVPSPSPAAQAEETKLTPE
                     EELALQKEKFEFTEEEINDFIEQLLPTFNALKEDVLDPKVIKSALTIKNTRDRASAIY
                     TGVLLTDLSEGEDGIPVKLTEAVLETAADLAKRSIKSPLGLGLLSCVATDEQGTSWLQ
                     CNIEDLRLFFTAVAAAKGKKSSEVLTTIFKSVYEQELDAEKKLRESIGIPVTDESEVK
                     EENTKIFTCSMGEMLLDAPIFRKPCRRFKPYVEYLERLKKEEEELRAMEALIEEMEAS
                     ENQSNSNSEKSETDSNSESDSDEETDANSEPEPSTEAAE"
     gene            complement(1618..2421)
                     /gene="proC"
                     /locus_tag="PLH57_06055"
     CDS             complement(1618..2421)
                     /gene="proC"
                     /locus_tag="PLH57_06055"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRK02211.1"
                     /translation="MANTIGILGLGKMGEALVRGMLDAGVAKNRILGTTGRAKSAAEA
                     SKRLGIKVFTDNQKLIEKCDVIFLSVKPQHVEAVLNENSKSLTKKKLLVSVCASITTS
                     QLESWSGGRCAVVRSMPNTPCLIGEGVTAIASSATSKPSDLKLVSELFETVGKVVVLD
                     ESLMDGATGLSGCGPAYVYLVIEALSEAGVKVGLPRKTATLLAAQTLVGAAKMVLERG
                     EHPAALKDEVTTPAGCTIDGLMALEAGGLRVTLINAVMAAAERSTKLSR"
     gene            complement(2447..>3041)
                     /locus_tag="PLH57_06060"
     CDS             complement(2447..>3041)
                     /locus_tag="PLH57_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02212.1"
                     /translation="YVDRAADLKMAVKILINAKMQRPGVCNSAETVLVHQTVAKRFFK
                     LFLTELGRQKKTLEIRACKRSHAALLANQRLAPGLNLRRASPKDWNTEHLELILNAAV
                     VSSAPAAQRHIATYGSRHSEAIVTKSRKTAEAFLNSVDAAAVYWNASTRFTDGHQLGL
                     GGEIGISTQKLHVRGPVGLEALTCQQWVLRGSGQIRI"
BASE COUNT          806 a          806 c          687 g          742 t
ORIGIN      
        1 tggcgaaaaa atacgagcga ttcaaaagaa aatttaagca ttatactgaa gactttagaa
       61 tcacgcgcga cccacaggct gcggatcgac tcacctcgct gcactttgcg acatctaaat
      121 ttcattccga aaccattcac ctattgttga gccttgccaa aaaaaccgtc gcagccaacg
      181 agtttggaga agttgctctc agtcagtttt tcgatttcgt agcaaaaaat gaagcaaggg
      241 cgagcgacga tgcattgctc attggcagtg aaatcacccg acctttgtac cttcatataa
      301 tagaccgtgt tgaagagctt ggtccggaag ggctgcagac gattgcagag actctctttc
      361 acactcaacg cgcgctctcg acgattgctt ccgctgattc agagcgttcg gctaagattc
      421 tgctattgcg cgacacggtt cgccctttac ttgaacattg ggaccaagcg ggtgaggcag
      481 aattcgacaa aagatcctat tatttcaatc gcttagaact cttagcggac gcagactgca
      541 attcgatcat ggcgaattag ccttcccgat tgtaggctcc aatgagcgat gacctatttt
      601 gtagtgattt agacggttat ttatggtaat ttccccccaa caaaacggag tgtgcttatg
      661 aatttaaagt tatctgctct ttgcctactt tcggcccttt tggggaacct ggcgctagct
      721 caacaagcaa ctccggtacc gagcccttcc ccggccgcac aagctgaaga aactaagctc
      781 actcccgaag aagaactagc tcttcaaaaa gaaaaattcg aattcaccga agaagaaatt
      841 aacgacttta tcgagcaact cctaccgacg ttcaacgcac ttaaagaaga cgtgctggac
      901 cctaaagtta ttaaaagcgc attaactatt aagaacacgc gagaccgcgc gtcggccata
      961 tacactggag ttttgcttac tgacctcagc gaaggtgaag acggaattcc ggttaaattg
     1021 acagaagcgg ttttggaaac tgcggccgac ctggctaaac gttccatcaa aagcccccta
     1081 gggcttggtc ttttgagttg cgtggcaacc gatgaacaag gcacttcttg gcttcaatgc
     1141 aacatcgaag acctgcgtct attttttacc gctgtggcag ctgcaaaagg taaaaaaagc
     1201 tcggaagtac ttacgactat tttcaagtcg gtttacgagc aagagctgga tgcagaaaaa
     1261 aaattacgcg aatctatcgg aattccagtg accgacgaat cggaagtgaa agaagaaaat
     1321 accaaaatct ttacctgctc tatgggcgag atgctgttgg atgcgccaat tttccgaaag
     1381 ccctgccggc gcttcaaacc atacgtggag tatcttgaga gactcaagaa ggaagaagaa
     1441 gagctccgcg caatggaagc actcattgaa gaaatggaag cctcagaaaa tcagtcgaat
     1501 tcgaatagtg aaaaatccga gacggactcc aattccgagt cggattccga tgaggagact
     1561 gatgccaact ccgagcctga gcccagcact gaagccgcgg aataattctg ccggccttca
     1621 gcgactcaat ttcgtgctgc gctccgctgc cgccatcacg gcattaatta gcgtcacgcg
     1681 aagcccgcct gcctccagcg ccatcaatcc atcgatagtg caccctgcag gggtcgtgac
     1741 ttcgtctttt agtgcggcgg gatgttcacc ccgctcgagc accattttag cggcacccac
     1801 caacgtctga gcagccagca acgtggccgt tttgcggggc agcccgactt taactcccgc
     1861 ctcagaaagt gcttcgatca ccaaatagac atatgccggt ccgcagccgc ttaaaccggt
     1921 agcgccgtcc atcaaagatt catcgagtac caccacttta cctacggtct caaacaactc
     1981 gcttaccaat tttaaatcag aaggtttaga ggtcgccgaa gacgcgatgg ccgtaacgcc
     2041 ctcgccgatc aaacacggag tattgggcat cgaccgcaca actgcacatc gcccgccact
     2101 ccaactctcc aactgactag tagtgatgga agcgcaaacc gaaactaaaa gttttttctt
     2161 agttaaactt ttagaatttt cgtttaaaac ggcctctacg tgctgcggtt taacacttag
     2221 aaaaattacg tcacactttt cgataagctt ctggttatcg gtaaacactt taattcccag
     2281 ccgcttcgat gcctccgccg cagactttgc tcgtccggta gtaccaagaa ttcgattttt
     2341 ggcgactccc gcgtcaagca ttccgcggac tagcgcttcg cccattttcc caagtcccaa
     2401 aatgccaatc gtgttcgcca tgttttaccc cttcttgttt ttactcttaa atccgaattt
     2461 gtccgctgcc tctcagtacc cattgttggc aagtaagcgc ttcaaggccc acaggccccc
     2521 gaacatgaag tttttgggta ctaattccaa tctcgccgcc caagccaagc tggtgtccat
     2581 cggtaaaacg agtagacgcg ttccaataga ccgctgccgc gtcgaccgaa tttaaaaatg
     2641 cctctgcggt ttttcggctc ttcgtaacta tcgcctcgga atggcgcgat ccgtaggtcg
     2701 cgatatgcct ctgcgccgcc ggggcgctag aaactaccgc agcatttaga atgagttcaa
     2761 ggtgttcggt attccaatct ttggggcttg ctcggcgcag attcagcccc ggagctaggc
     2821 gctggttcgc caacaatgca gcgtgagaac gcttgcaggc tcgaatctcc aaagttttct
     2881 tttgccgacc aagttcggtc aaaaacaatt taaaaaaccg ttttgccacc gtttgatgca
     2941 ccaaaaccgt ttctgccgaa ttacagactc ccgggcgttg catttttgcg tttattaaaa
     3001 ttttaacagc catttttaga tcagccgcgc gatcgacgta a
//
LOCUS       DAOMXO010000247         3035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18207_length_3035_cov_4.213087, whole genome shotgun sequence.
ACCESSION   DAOMXO010000247 DAOMXO010000000
VERSION     DAOMXO010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18207_length_3035_cov_4.213087"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PLH57_06065"
     CDS             <1..958
                     /locus_tag="PLH57_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRK02213.1"
                     /translation="RISIGGGGTDLPSYFERFGGFVISAAIDKYVYVALHKTFQKTYM
                     IRYSQFEAVDDVASIKHPIVREAFKLHDVGPFIEMVSMADIPAGTGLGSSGSFNVGLL
                     RGIYTMKREPVTNFGLADEACKLEIDILREPIGKQDQFIAAFGGITCFEFEKNGGVNV
                     APLRISNDSLADLEDNLLMFFTGYSRSASNVLSDQKKRSEVDDKEMLNNLHFIKDLGI
                     TIKKELEKGNCSEFGRLMHEHWLHKKKRSSGISSDQINRWYDVGFANGALGGKLIGAG
                     GGGFLMFYAKDRVALREAMAKEGLSEVRFRFDHHGSRVMVEN"
     gene            960..1967
                     /locus_tag="PLH57_06070"
     CDS             960..1967
                     /locus_tag="PLH57_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRK02214.1"
                     /translation="MNIGIIGAGLIGNKRANALPSTCKLTAVADLSNDRAHKLATAFG
                     ARAESSWAKLVSASDVDCVIVATTNDVIPDCAKAALKAGKHVLIEKPGGRFPDEVRSI
                     ADVAACSRGILRVGFNHRFHPGLQGAKKLLDSGVDGPLMYIRARYGHGARVGYEKEWR
                     GNPKLGGGGELLDQGVHLIDLCRWVGGSFELQYGHAQTFFWDMPVEDNGFLILRSPDR
                     KRSAFLHASCTEWKNLFDFEIFAKNAKIQIFGLGGSYGTEELRYYKMKPEMGPPDITV
                     TPFPGADESWKLEFQAFLSEVDGRKTDIGRIEDAVRAIEIVFDVYRSSGYDMERAICN
                     A"
     gene            1955..2710
                     /locus_tag="PLH57_06075"
     CDS             1955..2710
                     /locus_tag="PLH57_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRK02215.1"
                     /translation="MQCVILAGGLGTRMRGVTRDEIPKALIPVASSLGTEPFAFYQLK
                     WLASCGVTNVVYSIGFLGEQIRAVVGNGSRWGLTIKYVDEGKELRGTGGALRKAFDEK
                     VLDERFFVVYGDSFLPIELLPIWSAFAPMAAPALMTVLKNRNQWDSSNVIFENNNLRL
                     YKKNATPEEKSLMHYIDYGLSLIRRKTIATNIEADRKTDLAAVYEKLSVEGRLLGYEV
                     QERFYEIGSEQGLRDFEHWLKKQENIQWLSRLN"
     gene            2689..>3035
                     /locus_tag="PLH57_06080"
     CDS             2689..>3035
                     /locus_tag="PLH57_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006587261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRK02216.1"
                     /translation="MAFPLKLAIYADGANRDAMIKRYREGLVKGFTTNPTLMAKAGVR
                     DYKEFALSVLSEIKQMPISFEVFSDEFGEMEREALEIKSWASNVNVKIPITNTKSQSS
                     IPLIKSLLDKGTK"
BASE COUNT          823 a          638 c          783 g          791 t
ORIGIN      
        1 tcgaattagc atcggtggag gcggaacaga cctgccttcc tattttgagc gtttcggagg
       61 cttcgtaatt tccgccgcaa ttgataaata cgtttacgtt gcgctccata aaacttttca
      121 aaaaacctac atgattcgtt attcgcaatt tgaagcggta gacgatgtcg ctagcattaa
      181 acatccgata gtgcgggaag cgttcaaatt gcacgatgtc ggacctttta ttgaaatggt
      241 gagcatggcc gacattccgg ctggaactgg tttgggttcg tcagggtcct tcaatgtcgg
      301 gctcttacgc ggcatttata ccatgaaacg agagcccgta accaattttg gactagccga
      361 cgaagcctgc aaacttgaaa tagatattct acgtgaaccc atcggtaaac aagatcagtt
      421 tattgcagcg tttggcggaa tcacctgctt tgaattcgaa aaaaatgggg gagtaaatgt
      481 ggctcctttg cgaatttcaa acgattcttt ggcagatctt gaagacaatc ttttaatgtt
      541 tttcaccggg tattctcgca gcgcaagtaa cgtgttgtcc gatcaaaaga agcgctctga
      601 ggtggacgac aaagaaatgt tgaacaatct gcatttcatt aaagatttgg gaattacgat
      661 aaaaaaagaa ctcgaaaagg gtaattgttc ggaatttggt cgattgatgc atgaacattg
      721 gctgcataag aaaaagcggt cttccggtat atcgagtgat caaatcaatc gatggtacga
      781 cgtcggcttc gccaacggag cactaggtgg aaaattaatc ggtgcgggtg gcggaggatt
      841 tcttatgttc tatgcgaaag atcgcgtagc acttcgagaa gccatggcaa aagaagggct
      901 tagtgaagtt cggtttcggt ttgaccatca cggttcacga gtgatggtgg agaactagtt
      961 tgaatattgg aataatcggt gccggattaa tcggtaacaa acgtgcgaat gcgttgccgt
     1021 caacctgcaa gcttactgcg gtcgcagact tatcaaatga ccgcgctcac aaattggcca
     1081 ccgctttcgg tgctcgcgca gagtcgagtt gggccaagct cgtttctgcg agcgacgttg
     1141 attgcgttat agtcgcgacc accaacgatg taattcctga ttgtgctaaa gccgcactta
     1201 aggcaggcaa gcacgttttg atcgaaaaac cgggcgggcg gtttcccgat gaggtacgtt
     1261 caatcgccga tgtagcggca tgctcacgcg gaattctgcg cgtagggttc aatcaccgtt
     1321 ttcacccggg tcttcagggt gcaaaaaaat tgttggattc tggtgtagac ggcccattaa
     1381 tgtacattcg cgctcggtac ggccacgggg ctcgagtcgg ttacgaaaaa gagtggcgag
     1441 ggaacccgaa attgggtggc ggcggcgaac ttctcgacca gggagtgcat ttaattgatt
     1501 tgtgcaggtg ggttggtggt agttttgaat tgcagtatgg ccacgcccaa acattttttt
     1561 gggatatgcc cgtagaagat aatggattcc taattttacg ctcacccgat cgcaaacgtt
     1621 ctgcattttt acatgcgagc tgtacagagt ggaaaaatct ttttgatttc gaaattttcg
     1681 ctaaaaacgc caaaattcag attttcggcc ttggcggcag ttacggcact gaagaactcc
     1741 gttactacaa aatgaagcca gaaatggggc cgccggatat tacagtgact ccctttcctg
     1801 gtgccgatga atcttggaag cttgagtttc aagcgtttct atctgaagtt gacggacgaa
     1861 aaacagacat cggccgaatc gaagacgctg tacgggccat tgagattgtt ttcgatgtat
     1921 atcgttcaag cggttacgat atggaacgcg cgatatgcaa tgcgtaattc tagccggcgg
     1981 cctgggtacc cgcatgcgcg gcgttacgcg cgatgaaatt ccaaaagcat taataccagt
     2041 tgcgtcaagt cttggtacag agccctttgc gttctatcag cttaagtggc tcgcctcctg
     2101 tggagtaaca aacgttgtct attcgattgg gtttttgggt gaacagattc gagccgttgt
     2161 cggcaacggg tctcgctggg gccttacaat aaagtacgtc gacgaaggta aagagttacg
     2221 aggcacaggc ggcgcccttc gaaaagcctt tgacgaaaaa gttctcgatg agcgattttt
     2281 tgtggtttat ggggattcat ttttaccgat cgagttacta cccatctggt cagcgtttgc
     2341 tccaatggcg gcacccgccc tgatgacggt tttaaaaaat cggaaccaat gggattcaag
     2401 caacgttatt tttgaaaaca acaacctgcg cttatataaa aaaaatgcca caccggaaga
     2461 aaaaagtctg atgcattaca tcgactacgg actctcctta atccgacgta aaacgatcgc
     2521 cacaaacata gaggccgata gaaaaacgga cttggcagcc gtttacgaga agcttagtgt
     2581 agaggggcgc ttgcttggct acgaagtgca ggagcggttt tacgaaatcg gctccgaaca
     2641 agggcttaga gattttgaac attggcttaa aaaacaggag aacattcaat ggctttcccg
     2701 cttaaattag caatctacgc agacggcgcg aatcgcgacg caatgatcaa acgctatcgt
     2761 gaaggcttgg tgaaggggtt taccacgaat ccaacgctta tggccaaggc cggtgtgcgt
     2821 gattataagg aatttgctct ttcggttttg tctgaaataa aacaaatgcc catttcgttc
     2881 gaagtatttt ctgatgaatt tggcgaaatg gagcgcgaag cgctagaaat taaatcttgg
     2941 gcctcaaacg tgaatgttaa aataccaatc acgaatacga aatcgcagtc ctcgattcca
     3001 ttaatcaaat cgttactgga taagggcacg aaatt
//
LOCUS       DAOMXO010000248         3021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18331_length_3021_cov_4.882333, whole genome shotgun sequence.
ACCESSION   DAOMXO010000248 DAOMXO010000000
VERSION     DAOMXO010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18331_length_3021_cov_4.882333"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="PLH57_06085"
     CDS             <1..124
                     /locus_tag="PLH57_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HRK02217.1"
                     /translation="VKVGQVIPRSLYQAVAEILAYVYKLRGKKMGSQGPRPQSP"
     gene            237..2306
                     /gene="flhA"
                     /locus_tag="PLH57_06090"
     CDS             237..2306
                     /gene="flhA"
                     /locus_tag="PLH57_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRK02218.1"
                     /translation="MSRFIQNSDMVLAVGIVAILIVMVLPLPAFVVDILLSSSIALSV
                     VLLLAALYTQRPLDFSSFPSLLLLTTLFRLSLNVASTRLILLHGADRGVDAAGDVIRS
                     FGEFVVGGNYAVGIIVFIILVIINFIVITKGAGRVAEVAARFTLDAMPGKQMAIDADL
                     NAGVINEEEARKRRSEIAREADFYGAMDGASKFVRGDAIAGILIVLVNVIGGILIGTL
                     QQGKGLGEAASTYTLLTIGDGLVTQIPALIISTAAGMIVTRAASGQNLSREFTSQLTA
                     QPKAMMVAAFVMFVMAIIPGLPFLPFVTMSFAAGYLAYRLTEKEKSVAEVNRRREELE
                     KLKPQKEKLESLLPVDVLELEVGYGLISIVDSEQNGELLERITHIRKQFALDLGVIIP
                     PLRIRDNLQLKPGEYIIQLKGVTIAQGELMVDHWLAMDPGNASERIPGVPTTEPAFGL
                     AAIWITAKQKEQAQIAGYTVVDLSTVIATHLTELIRKHAHELLGRQEIQNLIDVVKQN
                     HPKVVEELIPTLLPVGTVLKVCQNLLREGVSIRDLRTILETLADHSAQTKDALLLTEY
                     SRSALARSITKKLLNSGGELPLITLERSLEEQVASGLIQTDQGPQLSLEPGFVQNLIR
                     ELNAQAERMVMVNSNAVLLVSPVIRAHIRQLVEKFIPHLVVLSHNEISPNIPIKSFST
                     VRMSHAG"
     gene            2296..>3021
                     /locus_tag="PLH57_06095"
     CDS             2296..>3021
                     /locus_tag="PLH57_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02219.1"
                     /translation="MQVKKFEAQTMQEALKLVKTELGPEAIILSTKNHKKGFGLLSKA
                     SVEVTAAVADRSLLKKKVTDKVMTGPINEQIQKFSASKQSEVYDRVGEYYIDKAKARI
                     QARREREERDAEKEQRRKERSQQARHSGASAQTANPEPRRYADIGDDDEAAVSKAQST
                     DAGLPSNYARNGRVFTEAPASYDTAPNNSNTYANYSSNESQHREKVLAAEVEQLKNIV
                     EELKNEQAMIADSRVAESAGDAVY"
BASE COUNT          794 a          713 c          782 g          732 t
ORIGIN      
        1 tgtgaaggta ggtcaggtga ttccccggtc gctgtatcag gccgtggcgg aaatcttggc
       61 ctatgtgtac aaacttcgtg gcaaaaaaat gggttcgcag ggaccccgtc cacaaagtcc
      121 gtgaaactaa ctgaccgaga taaaaggatt tatcggtggc agaagccaaa gtaggggcag
      181 caacagcaga cgcgaaaaag ggtgcagcgg gcgacgtaaa agtcggcaat ccgctcatgt
      241 cgcgcttcat tcaaaattct gacatggtac tcgccgtcgg aatcgtggcg atcttgatcg
      301 tcatggtttt gccacttccg gcatttgtag tcgatatttt gttgtcgagt tcgattgcgc
      361 taagcgtcgt acttttgtta gcggcacttt atacccagcg accgctcgat tttagctcgt
      421 ttccgagcct gcttttactc accacattgt ttcggctcag tctaaacgtg gcgagcacac
      481 gattgatttt gctgcacggc gccgaccgag gagtcgacgc agccggtgac gtgatacgaa
      541 gtttcggtga attcgtcgtt ggcggtaatt acgcggtcgg gatcatcgtt tttatcattt
      601 tggtgatcat taacttcatc gttatcacta aaggcgctgg ccgcgtcgcc gaagtagctg
      661 cacgcttcac gttggacgcg atgcccggca agcaaatggc gatcgacgcc gatttgaacg
      721 ccggtgtgat taacgaagaa gaggcccgaa aacggcgatc agagattgca cgagaggctg
      781 acttttacgg cgcgatggac ggtgccagta agttcgtgcg cggtgacgcg attgcgggaa
      841 tattaattgt tctcgtcaac gtgattggcg gtattttaat cggcaccctt cagcagggca
      901 aggggctggg cgaagccgcc agcacctaca ctttactcac catcggtgac ggtctggtaa
      961 ctcaaatccc tgcgttaatt atttcaactg ctgctggtat gatcgtcacg cgcgccgctt
     1021 cgggtcagaa tctctcgcgc gaatttacgt ctcagctcac tgcgcaaccc aaagcaatga
     1081 tggtcgctgc gtttgttatg ttcgtgatgg cgataattcc agggttaccg tttctgccgt
     1141 ttgtgacgat gtccttcgct gctggatact tggcatatcg actcaccgaa aaagaaaaat
     1201 cagtcgcgga agttaataga cgtcgtgagg aactcgaaaa actcaaaccc caaaaagaaa
     1261 agctcgaaag cctcttgccg gtcgatgttc tcgaactcga ggttggttat ggtttgattt
     1321 cgattgtaga ttctgaacaa aacggcgaac tccttgaacg tatcactcac attcgtaaac
     1381 agttcgcgct ggatttaggt gtaatcattc caccactgcg aattcgcgat aacttacagc
     1441 ttaaaccggg cgagtacatt atccagttaa aaggcgttac gattgctcag ggtgagctca
     1501 tggtcgacca ttggttagcc atggaccctg gcaatgcttc tgagcgaatc cctggagttc
     1561 ccaccactga accagcgttt ggattggctg cgatttggat taccgccaaa caaaaagaac
     1621 aagctcaaat cgcaggttac accgtggtgg acttatctac tgttattgcc acccacttga
     1681 ccgagctaat tcgtaagcat gcgcatgaac ttttgggacg tcaggaaatt caaaatctaa
     1741 tcgatgtcgt gaagcaaaat cacccgaaag tggtggaaga actgattcca acgctgcttc
     1801 cagtgggtac ggtattgaaa gtatgtcaaa atcttttgcg tgaaggggtg agtattagag
     1861 accttcgcac tattcttgaa acactggctg atcattccgc acagactaaa gatgcccttt
     1921 tgttgaccga gtactcgcga tcagctctcg ctcggagcat taccaaaaag cttcttaatt
     1981 cgggcggcga gttaccgttg atcacgttgg agcgatcatt agaagaacag gtcgcttcgg
     2041 gattgattca aaccgatcaa gggccgcagt tgtcgcttga gcccggtttt gttcagaatc
     2101 tcatacggga acttaacgct caagcagagc gaatggtgat ggtgaactcc aacgcggttc
     2161 ttttggtgtc gccggtgatc cgcgctcaca ttcggcaatt ggtcgaaaaa tttattccac
     2221 atttggtcgt gctatctcac aacgagattt cacccaatat tcctattaag tctttttcca
     2281 cagtgaggat gagtcatgca ggttaaaaag ttcgaggccc aaacaatgca ggaagctctg
     2341 aagcttgtta aaaccgagct tggacccgaa gccataattt tgtcgactaa aaatcacaaa
     2401 aaaggatttg ggctgctctc gaaagcatcg gtcgaggtta ccgcggcggt ggccgatcga
     2461 tcgcttctta agaaaaaagt gaccgataaa gtcatgactg gtccgattaa tgagcagatt
     2521 caaaaattct cggcgagtaa acagtcagag gtttacgacc gagtaggtga atattacatc
     2581 gataaagcaa aggctcggat tcaagctcgt agagagcgcg aagagcgcga cgcagaaaaa
     2641 gaacaacgca gaaaagaacg aagccaacag gctcgccatt ctggtgcaag tgctcaaacc
     2701 gctaatcccg agccgcggcg ctacgcagac atcggtgacg acgacgaagc tgcagtctcg
     2761 aaggcgcaat caacggacgc agggcttccg tcgaactatg ctcgcaacgg aagggttttc
     2821 actgaggcac ctgcttctta tgacactgcc cctaacaaca gtaataccta tgcgaactat
     2881 tcttctaacg agagtcagca ccgagaaaaa gtactcgcag cagaagtgga acaactgaaa
     2941 aacattgttg aagaactcaa aaacgagcag gcgatgattg cagattctcg cgtcgcagaa
     3001 agtgcgggcg atgcggttta t
//
LOCUS       DAOMXO010000249         3020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18336_length_3020_cov_4.229680, whole genome shotgun sequence.
ACCESSION   DAOMXO010000249 DAOMXO010000000
VERSION     DAOMXO010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18336_length_3020_cov_4.229680"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1649
                     /locus_tag="PLH57_06100"
     CDS             114..1649
                     /locus_tag="PLH57_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02220.1"
                     /translation="MQIRKLTRFFAAPMLATTLLVCMFDDANAIQMDWGGQFWFDNHW
                     LNNYQLDRGRPGYDNDPTYDNGSPYVAGAGEKNAIWYSAFMKLNPKLIVNDNIQLKSE
                     WHVGSPIYGFFGRGYPSSGDERYNFTGAQKDNFTIGAQRFWANLITDFGTIELGRAPI
                     HWGLGAVWDSGDDLFERYQNTGDMVRLTAKFGNFLVQPALSKVAVGNNVAGASTSTGA
                     TLQGNDDVTDYNLAVKYDNTEEDFEFGVMWTKRTGNTAQNTVYFNRANTGSNRINFNT
                     FDFFARKKWSRFSFGAEFPLFTGDIGAVDQSSAIEYQYKTYALILESGYNSDVWDIKF
                     RLGHVPGQPPTGAGDERFRAVYLNKNYDLGLIMFNYNLHGLAQNNPDTVANASLNSPY
                     DNQIVNANYLALLPELKLDKWSLKGGVIVGFADEVAEAGRRLYSYHKRRFYASNGNQS
                     EFLGWEADIGTSFRWDENFVVSWDIGFFFPGSYYEFSNFANNSRLNTSMMFASRVQAG
                     ITF"
     gene            1794..2630
                     /locus_tag="PLH57_06105"
     CDS             1794..2630
                     /locus_tag="PLH57_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02221.1"
                     /translation="MDHRLEAVQDATRYSDGLYLKLQAKGNSKEPVRDEEADRMAMRE
                     SVRALLSEETRQLTELYDRLRALNVEAVESELLEKFVQQRRRLEPVYEYVRFKNGGPV
                     SLPLPADKEIPRITLRELVDIKVTLQTLLLQSHKLNRELDESRFVMRRDAGSESAMFR
                     TSAAVLLTGAAALIGDLPAWAIAPAAFTGGAFTLFAPRAAGKVSESRLPMKLILLTRK
                     ELVDELGNLTKLMSEHFKFHPVHWISQINGNVCKEFLNPETAKLEVPGSSPWDAMPSI
                     VE"
     gene            complement(2617..>3020)
                     /locus_tag="PLH57_06110"
     CDS             complement(2617..>3020)
                     /locus_tag="PLH57_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRK02222.1"
                     /translation="VYTKLLFKRPVITEESELQKKLEESHRPLVPEQKPTVDFEQLMV
                     NIAMTSGKAHYATIAWAVECRDDEVKRMVEEKKDLFVDRVISTLGKRQITELNTIQGK
                     LLLKTELMGKFNELTKPAGITDIYFNTFILQ"
BASE COUNT          773 a          678 c          752 g          817 t
ORIGIN      
        1 ccgccaccag aagccaggcg atggaccttc gaatcccgaa tatcagagta gacatgtggt
       61 ttctctccat ctatatctaa aacccacaat gttcaaaata acgagggaaa tttatgcaaa
      121 tccgtaagct tacgcgtttt tttgccgcac cgatgttggc tacaacattg ctcgtgtgca
      181 tgtttgacga cgcgaatgcg attcaaatgg attggggcgg ccagttttgg ttcgacaatc
      241 attggttgaa caattatcag ctcgatcgcg ggcgcccagg ctacgacaac gatcccacgt
      301 acgacaacgg cagtccctat gtggccgggg ccggtgagaa gaacgcgatt tggtattcgg
      361 cgtttatgaa gctgaatcca aaattgatag tgaacgacaa tatacagctt aaaagcgaat
      421 ggcacgttgg aagccccata tatggcttct tcggacgcgg gtatccgagc agcggcgatg
      481 aacgttataa ttttacgggc gcacagaaag ataattttac gatcggagct caacgttttt
      541 gggctaattt aatcaccgat tttggaacaa ttgaacttgg tcgagcaccc attcattggg
      601 gcctgggagc ggtctgggat tctggggacg acctctttga acgttatcaa aacaccggcg
      661 atatggttcg attgactgcc aaatttggca attttttagt ccaaccagct ctgtcaaaag
      721 tagcggttgg caacaacgtt gctggcgcta gcacttcgac cggcgctacg ctgcaaggta
      781 acgatgatgt taccgattac aatttggccg taaaatacga caacactgaa gaagattttg
      841 aattcggcgt catgtggacg aagcgaacgg gcaatactgc gcaaaatacc gtttatttca
      901 accgggccaa tacgggttcg aatcggataa attttaatac cttcgacttt tttgctcgaa
      961 aaaaatggag ccggttttcg tttggtgcgg aatttccgct ctttacgggc gacattggcg
     1021 cggtcgatca atcttcggcg atcgagtatc agtataaaac ctacgctttg attttagagt
     1081 cgggttataa ttcggacgtt tgggatatca aatttcgact gggtcacgtg cccggtcagc
     1141 caccgacggg cgctggggat gaacgttttc gcgcggttta cctgaacaaa aactacgatc
     1201 ttggtttgat catgtttaac tacaatcttc atggtctggc ccaaaacaac cccgacactg
     1261 ttgctaacgc gagcctcaat tcgccctatg acaaccaaat tgtgaatgcc aattacctgg
     1321 cgttgttgcc ggagctcaag ctcgataagt ggtccctaaa ggggggggta atcgtagggt
     1381 tcgcagacga agtggctgag gctggccgaa ggctatattc ttaccacaag cgtcgttttt
     1441 acgcctctaa tggcaaccaa tcggagttcc tagggtggga agcggacatt ggaacctcct
     1501 ttcgttggga cgagaatttc gtcgtaagct gggatattgg gtttttcttc cccggctctt
     1561 attacgagtt ttcgaacttt gctaataact cccggttgaa cacgagtatg atgtttgcct
     1621 cgagggtgca ggccggaatc acattttaat cggctgttaa tgggtttgtt aatccatagt
     1681 tgactaaaat tattttatcg tgataccccc acccaatgtt tttgcggttg gtgggtcagt
     1741 tcagcacaat tggtattttg tcgttagtcc ctattttggg gctagctggg ccggtggacc
     1801 accggcttga agccgttcaa gacgccactc ggtacagcga cgggctctat ttaaagctac
     1861 aagctaaagg aaattcgaaa gagccagtgc gcgacgagga agccgatcga atggcgatgc
     1921 gcgaaagtgt gcgggcgttg ttatccgaag agactcgcca actgacggaa ctttatgatc
     1981 gacttagagc gttaaacgtt gaagctgtag aaagtgaatt acttgaaaag ttcgttcagc
     2041 aacgtaggcg attagagcca gtttacgaat atgttcgatt caaaaatggt ggtccggtct
     2101 cgcttccgct accggccgat aaagaaattc ctcgaattac gctccgagaa ctagttgata
     2161 taaaggtgac gcttcaaaca cttttactgc aatcgcataa actaaatcga gaactcgacg
     2221 aatctcgatt cgtaatgcga agagacgcag gtagcgaaag cgccatgttt cgaacgagtg
     2281 ccgcggtcct attaaccggc gcggccgctt tgatcggcga tttacctgct tgggcaatcg
     2341 cgcccgctgc ttttacgggt ggtgcattta ccctttttgc gccacgtgcc gctggcaaag
     2401 tgtccgagag ccgtttgcca atgaaattga ttttgctgac gcgcaaagag cttgttgatg
     2461 agctaggaaa tttgacaaag ttgatgagcg aacattttaa atttcacccc gtgcactgga
     2521 tcagccaaat caacggcaat gtgtgcaagg aatttctaaa tcccgaaaca gctaagctag
     2581 aagtccccgg cagttctccg tgggatgcaa tgccttctat tgtagaataa acgtattaaa
     2641 atagatgtcc gtgattccgg cgggcttggt aagttcgttg aactttccca taagctctgt
     2701 ttttaacaat aacttaccct gaatggtgtt aagttcagtg atctgccgct tacccaacgt
     2761 tgaaattacg cgatcgacaa acaagtcttt cttttcttcg accatgcgtt ttacttcgtc
     2821 gtcacggcac tcaacagccc acgcgatcgt ggcataatga gccttgccag aagtcatagc
     2881 gatattgacc attaattgtt cgaaatccac agtcggtttt tgttctggaa ccaacggcct
     2941 atgtgattct tctagttttt tctggagctc ggattcttca gtaataacgg gtcttttaaa
     3001 aagtaatttc gtgtaaacaa
//
LOCUS       DAOMXO010000250         3019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18344_length_3019_cov_5.871795, whole genome shotgun sequence.
ACCESSION   DAOMXO010000250 DAOMXO010000000
VERSION     DAOMXO010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18344_length_3019_cov_5.871795"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="PLH57_06115"
     CDS             complement(<1..1366)
                     /locus_tag="PLH57_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRK02223.1"
                     /translation="MRRRDFLKTLSGVSATLVLSSSPLLVSACAGRSAKRNTRSTFDL
                     SSVSPVDRSDHSENPTYVAGDDPAVPHEVLWNKNAFLASKTISGDSEVAPLVIVGGGM
                     SGLFTTYLLRDLNPILLEQATRFGGNSKAESWNGLDYSVGAAYFVKPELGTPWQKLLT
                     ELEVDKLWRVKTGEDPVALESNIYERFWSGETARSQANKFRALEQYFQNVFNDQNGEV
                     YPDIPYNDAQMRAVVTGLDQESFHSLVRRKCGGRIPTHIETVIEHYCWSTFGASSAEL
                     SAAAGLNNFAAEFGDLVVTPGGNAGVAERVLERLSQSIEPSRLRTQSLVVDVQARGNY
                     NEVVYLDAGRDLKKIKAKAVVLACPKFVAKRLVDTFESDRMNAISKMTYRAYLVANVF
                     LKKAPQKVFYDLYMLGNGTIDLRNVMTTSLQKKATDVVLGNFAYGGDASNQAVLTLYR
                     AFPFE"
     gene            complement(1380..2459)
                     /locus_tag="PLH57_06120"
     CDS             complement(1380..2459)
                     /locus_tag="PLH57_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRK02224.1"
                     /translation="MTSIVLRLNLVGLFILLASAGSVQGADASRTNVILITLDGVIAK
                     DFYNPKLLKEFWKRNSEKSLKWGGSKDSLGTAEVLNKAYISMPGYFSIFAGKETDCQT
                     NDCERIQTETFPEKIVRELGLAKTDAVAVTSWERLPRAIESRAGTVLVNSGFQNAPSE
                     TLLACSEISSELDLLASAQSLDRPTWEQNRFDKYTWAFANLMFDCHKPRLLYVSLNDT
                     DDYAHLGERENYEAALKGYDQWLVDFQKRLEKLELYKNNTLVILASDHGRGEGDQWQR
                     HNSNLETAKNTYLTLIGIGTGIDLAALKKKYAGPVNHSHIRQIIEDVMLPVHASEPVK
                     EKTGESSTLEIVVEQKPDVPTENAN"
     gene            complement(2473..2841)
                     /locus_tag="PLH57_06125"
     CDS             complement(2473..2841)
                     /locus_tag="PLH57_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02225.1"
                     /translation="MISYQIYKLMHLTGILMIFLGLGGVAMHAINGGPRAHRWKRGVA
                     LTHGVGMLLTLVGGFGLLARLGMAANMPGWAYAKIVIWLGLGGALALFIRKQSCAKKA
                     WFGVLVLGLAAAYLANYKPF"
BASE COUNT          752 a          762 c          728 g          777 t
ORIGIN      
        1 cttcgaacgg aaacgcacgg tacaaggtta aaaccgcttg gttcgacgcg tcgccaccgt
       61 aggcaaaatt ccccaacacc acatcggtgg ctttcttttg aagactggtg gtcatgacgt
      121 tgcgcaggtc tatggttcca ttgccaagca tgtagagatc gtaaaaaact ttttgtggcg
      181 cttttttaag aaaaacattc gccactagat atgcacggta agtcattttg gaaattgcat
      241 tcattcgatc tgactcgaat gtgtccacta aacgtttagc tacgaactta gggcaagcaa
      301 gtaccaccgc tttggctttg atttttttta gatcacgacc cgcgtcgaga tacactactt
      361 cgttgtaatt acctctagct tgaacgtcga caactaggct ttgagtccga agccgagacg
      421 gctcgattga ttgggatagg cgttctagta cacgttcggc cactcccgca tttcctcctg
      481 gtgtaacaac caagtcgcca aactctgcag cgaagttatt tagccccgct gccgcgctta
      541 gttcagcgct agaagctcca aaggtagacc agcagtagtg ctcaatcacc gtttcgatgt
      601 gggtgggtat gcgaccgccg cattttcgac gaacaaggga gtgaaaagat tcttgatcga
      661 gcccggtgac aaccgcccgc atttgcgcat cgttgtaggg tatatcgggg tacacctcgc
      721 cgttttggtc gttaaaaaca ttttgaaagt actgttctag tgcccgaaat ttattggcct
      781 gagacctagc ggtctcgccg ctccaaaagc gctcataaat gttcgattca agcgccactg
      841 ggtcttcgcc ggttttgact cgccaaagtt tgtcgacttc aagttcggtc agaagcttct
      901 gccaaggcgt tccgagctcg ggttttacga agtaggcggc acccactgaa taatcgagac
      961 cattccaaga ctctgctttc gaattaccgc caaatcgcgt tgcctgttcg agcaaaatcg
     1021 gattgagatc acgcaagaga taggttgtga acaatccgct catgccgccg ccaacgatca
     1081 caagtggcgc aacttcagaa tctccagata tggttttaga tgctaaaaat gcgtttttat
     1141 tccagagcac ttcatgaggt actgctgggt catcgcctgc gacataggtc ggattttcac
     1201 tatgatctga acggtccacc ggcgaaacag aagacaaatc gaaggtggaa cgggtattac
     1261 gtttggcgct tcgccccgca caggccgaaa ccaaaagcgg cgaactagaa agtacgagcg
     1321 tggctgaaac tcccgacaga gtctttaaaa agtcacgacg cctcatctta cccctagagt
     1381 cagttggcat tttcagttgg cacgtctggc ttctgttcga ccactatttc cagcgtggaa
     1441 gattctccag ttttttcctt caccggttct gatgcgtgta cgggcagcat cacatcttcg
     1501 attatttgtc gaatgtggga gtggtttacg gggcccgcat attttttctt gagcgccgca
     1561 agatcaattc cagtgccgat cccaataagt gttaagtaag tgtttttcgc agtttcgagg
     1621 tttgagttat ggcgttgcca ctgatcgcct tcgccacgcc catgatcact cgcaagtata
     1681 acgagcgtgt tgtttttata gagttccaac ttttccaggc gtttttggaa gtccaccaac
     1741 cactgatcgt agcctttaag ggctgcctca tagttttcgc gctccccgag gtgcgcgtag
     1801 tcatcggtgt cattaagcga aacgtaaagc aacctaggtt tgtggcaatc gaacatcaag
     1861 tttgcgaaag cccaggtgta tttgtcgaat cgattttgtt cccaagttgg acgatcgagc
     1921 gactgtgcgc ttgcaagtag gtcgagttcg ctactgattt cggaacacgc cagcaaagtt
     1981 tcgctcggag cattttggaa tccggagttc accaatactg tgcctgcccg actttcaatc
     2041 gctcggggca ggcgttccca agacgttacg gccactgcat cggttttggc cagccctagt
     2101 tctcgaacaa ttttttcggg aaacgtttcg gtttgaattc gctcacaatc gttggtctgg
     2161 cagtcggttt cctttccggc gaaaattgaa aagtaaccgg gcatgctgat gtaggctttg
     2221 ttcaacactt cggcggttcc aagtgagtct ttactcccac cccacttaag acttttctct
     2281 gaatttcgtt tccagaattc tttgaggagt ttgggattat aaaaatcttt agcaatcaca
     2341 ccatctagcg tgatgagaat tacgttggta cgagaggcat ccgcaccttg caccgatccc
     2401 gcagaagcca ataaaataaa taggccaacc agattaagac gtaaaactat tgaagtcacg
     2461 aatccccccc aatcaaaacg gtttgtagtt cgctagatag gctgcagcca aaccgagaac
     2521 aagcacccca aaccaggctt tttttgcaca ggattgtttg cgaataaata gcgccaacgc
     2581 gccgcctaat cccagccaaa taacaatttt ggcataggcc cagcccggca tgttcgcagc
     2641 cattccgagg cgcgctaaga gaccaaagcc ccctaccaac gtaagcagca tacccacacc
     2701 gtgggtcaac gccacgcccc gtttccagcg atgggctcgg gggccaccgt taatggcatg
     2761 catggccacg ccgccaagtc ccagaaaaat catgagaatt ccggtaagat gcatgagttt
     2821 gtaaatctgg taagaaatca tatgcgcccc tcttaattta ggggtgaacc tagccgcaac
     2881 tgttcgaagg gtctattaaa aacgaaaggc ccgtgataac caaacggaat tcgatctgtc
     2941 ttcggagagg tgtctgagtg gttgaaagaa ccggtcttga aaaccggcat acgggaaacc
     3001 gtatcgtggg ttcgaatcc
//
LOCUS       DAOMXO010000251         3010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18413_length_3010_cov_6.118105, whole genome shotgun sequence.
ACCESSION   DAOMXO010000251 DAOMXO010000000
VERSION     DAOMXO010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18413_length_3010_cov_6.118105"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1597
                     /locus_tag="PLH57_06130"
     CDS             74..1597
                     /locus_tag="PLH57_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02226.1"
                     /translation="MSFLRKSTLALVLVTVSVVTSGNTACQLGSDPGVDAGGLAPANY
                     NLDADIPTEYLTEAQAEAFDNLREYPITDIRQIRMAHGESIYTASTNLDVIGYNPGAG
                     WWDHTLNYPAYHGLGEEAFFHLSSWYPQADFWPTAPKLEYLMIRTLGRGWDLNTDSRW
                     QTLEKDNITNEPAQDGLGYVFGNFEPNTQYTDQEGPCNFRNIRGVDAIGLMRYLMHWS
                     GLEVRGDTPEKLVSTLNDTLETFDSSQDRGKRWDVRFSDVGPAINYQVESQSLSGDIV
                     VWPNHIGMVFQLMNGSRFLFQSNGYHLNETDCLANYGQKRGPRVLRLSDVTNILGQPT
                     AVYRMDSLYNGTWEMSVRCEGAAENVAEVQFDLNTHDDLSTVLNFSGVDGGDNYSLAV
                     EMNYLHERGLLHGYIRANNLSRPETPTRVDIFGIYPGGLTDLDSGFFESFLQEPTDGS
                     VCMGEVRFRKVTPAQAKAYKAKLSGKKNKGKLKLGKLKSRKALSKGDRGEAGLPFLK"
     gene            complement(1613..2770)
                     /locus_tag="PLH57_06135"
     CDS             complement(1613..2770)
                     /locus_tag="PLH57_06135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRK02227.1"
                     /translation="MKAKKTRVIHYCNQLSLGGTEKTMELFCKYLPKEEFEVFAVSRL
                     HRPPTTQKIRVSLGALVGHKKSIAKQKLWRSMNARIPNFQSILGPDKVRFAESDQDLR
                     KIILEIGPDILHVHYSGNSEPPISDPEIMKKVPAVITTNQFEKENVSPSHQLVRRILF
                     VSNWLLQNKATWAKNDPRTTVVYNPVEKPLTNDDLRSELGISKDTFVIGRVGRPDPGI
                     HDPISLDAYARIETDKTLFLALSPPDNMVAQARELKLKNFRVLPPTPDPLWLSKFYNT
                     IDVLAHARKDGETFGCNIAEAMVHGKPVVSHISRVMDAQTEVIGNCGYIAPYGDQDAY
                     AEYLRQLRDNSTNRSELARQAKQRALDEFEAERLTLRLSEIYKNELAKPPR"
BASE COUNT          754 a          651 c          762 g          843 t
ORIGIN      
        1 cattgttcaa agttaaatgg tctgttatta tccggcgcat tagttaatcg ttaatttctt
       61 gaggtatcga gttatgagtt ttcttagaaa aagcacttta gcgttggtgt tggttactgt
      121 ctcggtagtg acgtcgggca ataccgcctg ccaattagga agtgatcctg gtgttgatgc
      181 aggtggattg gctcccgcca attacaatct cgatgctgat attccaacag agtatctcac
      241 cgaggcccaa gctgaagcat tcgataatct acgagaatat cctattaccg atatcagaca
      301 aattcgcatg gcccatggcg aatctatcta cactgcctca acgaatttag atgtcattgg
      361 ctataaccct ggcgctggtt ggtgggacca cactctaaat taccccgcct atcacggcct
      421 cggggaagag gccttttttc accttagttc ttggtaccca caggctgact tttggccaac
      481 agctccgaaa ttagaatatt taatgattcg tactttgggg cgtggctggg acttgaacac
      541 cgatagtcga tggcagacgt tagagaagga caacattaca aatgagcccg ctcaagatgg
      601 cttaggctat gtgtttggta actttgaacc gaatactcaa tatacggatc aagaggggcc
      661 gtgcaatttt cgtaatatac gcggtgttga tgcgattggg ttgatgcgct acttaatgca
      721 ctggtccggt cttgaggtta gaggcgatac tccggaaaag ttggtctcaa ctctcaatga
      781 taccctggaa acttttgata gcagtcaaga tcgcggaaaa agatgggatg ttcgctttag
      841 cgatgtcggg ccggcgatca actatcaagt tgaaagtcag tccctttctg gtgacatcgt
      901 tgtatggcca aatcacattg gaatggtctt tcaactgatg aacggctcac gctttttgtt
      961 tcagtcgaac ggatatcacc tcaatgaaac agactgtctc gcaaattacg gtcaaaagcg
     1021 tggtcctcgc gttctgcggt tatccgacgt caccaacatc ttaggccaac ccactgcggt
     1081 ctaccgtatg gactctttgt ataacggcac ttgggaaatg tcggtgcgtt gcgaaggtgc
     1141 ggcagaaaac gtcgctgaag tgcagttcga tctgaacact cacgatgact tgagtacagt
     1201 tttgaacttc agcggtgtag atggcggaga taactatagt ttggcggtgg aaatgaacta
     1261 tctgcacgag cgaggcctgc tccacgggta cattcgagca aataatttaa gccgccccga
     1321 aacacctacc cgagtagata tttttggcat ctaccctggc gggctcacgg accttgatag
     1381 tggttttttt gagtcgttct tgcaggaacc aactgatggt tcggtgtgta tgggcgaagt
     1441 tcgatttcgc aaggtaaccc ctgcccaagc gaaagcttat aaggcgaagc tttctggtaa
     1501 aaagaataag ggcaagctga agctcggcaa attgaaaagc cggaaggccc tttctaaagg
     1561 ggatcgtggc gaagccggat tgccgttttt aaagtaaaaa ataagactta gtttaccttg
     1621 gcggcttggc gagttcattt ttgtagatct cgctgagtcg cagggtaagt ctttcagctt
     1681 cgaactcgtc tagtgcccgt tgtttcgctt gtcgtgcgag ttcggagcga ttggtcgaat
     1741 tgtcgcgcaa ttgccttaaa tactcggcgt aggcgtcttg gtcgccgtag ggtgcgatat
     1801 aaccacaatt acctatgacc tctgtttgcg catccataac ccttgaaatg tgagagacga
     1861 ccggtttgcc gtgcaccatg gcttcggcaa tgttgcagcc aaaggtttcg ccgtctttgc
     1921 gagcatgcgc aagaacgtcg attgtgttgt aaaacttcga taaccagagc ggatctggtg
     1981 tgggcggtag aactctaaag ttcttgagct tcaactcgcg ggcctgggca accatgttgt
     2041 cgggcggcga caaggcgaga aagagagttt tgtcggtttc aatccgggcg taagcatcga
     2101 gggaaatcgg gtcgtgaatt ccggggtctg gtcgaccaac tctaccgatt acaaatgtat
     2161 ctttagaaat tccaagttcg cttcgcagat catcattggt aagcggtttt tcgacgggat
     2221 tgtaaaccac ggttgtgcgc gggtcattct ttgcccaggt tgctttgttt tgcagcaacc
     2281 agttcgaaac aaaaagaatt cgccgtacta actggtgcga tggtgagacg ttttcttttt
     2341 caaactgatt ggtggtgatg actgctggca ccttcttcat aatttcggga tcggaaattg
     2401 gtggttccga attccctgaa tagtgcacat gcaaaatatc gggacctatt tctagaatga
     2461 tttttcgcaa gtcttgatca gactcagcaa agcgcacttt gtcgggaccg agaatagatt
     2521 gaaagttggg aattcttgcg ttcatagaac gccagagctt ttgttttgcg atagatttct
     2581 tgtgaccgac aagagcacct aaggaaactc tgattttctg agtagttggc ggccgatgaa
     2641 gtcgagacac cgcaaaaacc tcgaactctt ctttgggtag atatttacaa aaaagctcca
     2701 tcgtcttttc tgttccccca agacttagtt gattacagta gtgaattact ctggtttttt
     2761 tcgctttcat cacaggcact ttacatacaa cattccagct tgcgtggcta ttacttgact
     2821 aaaacacccg taaatagagc gttttttacc gcaagcactg ttgagtcata ctaaagtgtt
     2881 ttgcttttga caccgaatgg ttttgcaaat gatgagtcgt caatttcaca acatgagagt
     2941 tgtgaaaagc gcacaaagtc attcgcgagt ggtgcacgga gtggcccatt cgaaacttca
     3001 aacacggagg
//
LOCUS       DAOMXO010000252         3005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18457_length_3005_cov_4.366780, whole genome shotgun sequence.
ACCESSION   DAOMXO010000252 DAOMXO010000000
VERSION     DAOMXO010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18457_length_3005_cov_4.366780"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2558)
                     /locus_tag="PLH57_06140"
     CDS             complement(<1..2558)
                     /locus_tag="PLH57_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRK02228.1"
                     /translation="MAGDQEVEESNNGSEKIDNGKTPKILLSILRFGFIGLVLSTLAI
                     CVVGGAVFLYFSSDLPALFTLEDYKPRIVTTIRAKDGTGIGEHFEERRYVLPPDQIPE
                     LAVKAFVAAEDDRFFEHQGIDFHGILRAAIANFKAGRVVQGGSTITQQVAKSLLLTPE
                     RSFVRKFKELILANRMERNFNKKQILYLYLNQIYLGQGAYGIEAAARIYFNKNAKDLT
                     LAESALLGGLPQAPSKYSPLQNPKSAKQRQLYVLRRMMESGAISVRQMEEASVQPVNL
                     YYTQDYNLKYSPYYVEHVRKYLLEKYGKEKLYEEGLEVLSPIDARLAEVGTKAVRRGV
                     RTVDRRLGYRGPIKKIKIRSEEASAIAEIQHFNLEKKFPYAVLVDEGVLSTDIKYYSA
                     QLSKPADAFQEDELYQGLVVQVDDKKKEVLVDLGVTKGIILIEDMRWANPNRSGASAV
                     EAINFPSQALSRGDVIWVSLLAKEMKDRPEYLSLALEQVPLVQGALISLDVRSGDVIS
                     MVGGYDFKESEFNRAIQAERQTGSAYKPIIYSAAIDKGYTPATIIQDSPIVFDAGENE
                     KWKPDNYEEKFYGDTLFRSALIHSRNIPTIKIVQDIEVPYLVEYSIRLGLPDKFNRDL
                     SIALGSSTASLLDLASVYAVFPRGGRKLRPIFITEVRDRDGNLLEENRSPPFIFPQSK
                     NLSTTIPPVLVAGELETRRAAGGADAEVPAWTPDESDPDLVMDPRTAFVMTHLMNEVT
                     TFGTGHAVKNLGRPAAGKTGTTNDYLDAWFMGFTPQIVTGVWVGFDTQKSLGGRSAGA
                     NTALPIWMDYMREATLDQPIEEFAVPNGVTFSFINTKTGKPTVANAPGSIREVF"
     gene            complement(2537..>3005)
                     /locus_tag="PLH57_06145"
     CDS             complement(2537..>3005)
                     /locus_tag="PLH57_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02229.1"
                     /translation="ISVKDLAVSKIPIIVVSSTENGPIFERQGNPIEILRGALGLPNI
                     IETIKVGGLTRSNAAFEIPFPSDYARDLGYGKTLCIDVVGRGNNFTPKENYEHQIAAQ
                     MRTVAALSKEQLKACDEVVRIPTDGIGYFDFEQKAELIFRGKAGVQKWLATKK"
BASE COUNT          759 a          749 c          625 g          872 t
ORIGIN      
        1 ataaacactt ctcgaattga accgggcgcg ttcgcgacgg ttggctttcc agttttggtg
       61 ttaataaagg aaaacgtcac accatttggt accgcgaact cttcgatcgg ttgatctaac
      121 gttgcctctc gcatgtagtc catccagatc ggaagtgctg tgttggctcc ggcagaacgt
      181 ccaccgagac tcttctgggt gtcaaatcca acccaaactc ccgtaacgat ctgaggggta
      241 aaacccataa accacgcatc gagatagtcg ttagtggtcc ccgtttttcc agcggctggt
      301 ctgcctagat tctttacggc gtgacccgtt ccgaaggttg tgacctcgtt cattaagtgg
      361 gtcatgacaa aagctgtacg agggtccatc acaagatcag ggtcggattc atctggcgtc
      421 caggcgggca cttcggcatc tgctcctccc gcggcccttc gcgtttccaa ttctccggcg
      481 actaataccg gcggaatagt agtcgataaa tttttggact gcggaaaaat aaatggcggc
      541 gaccgatttt cctccaataa attaccgtcg cgatcacgca cttcagtaat aaatattggt
      601 cttagttttc gaccgcctct tggaaaaaca gcgtaaaccg aagcaagatc caacagcgag
      661 gcagtcgacg accctaatgc tattgataaa tctctattga acttgtcggg cagacctaag
      721 cgaatcgagt actctactaa atatgggacc tcgatatcct gaacgatttt aatcgtagga
      781 atatttcgtg aatgaataag cgctgatcga aaaagagtat caccgtaaaa tttttcttcg
      841 taattatctg gtttccattt ttcattttcg ccggcatcaa aaacgatcgg ggaatcctga
      901 atgatcgtag ctggtgtata tcctttgtcg atggccgccg aataaatgat tggtttatag
      961 gctgatcccg tttgccgctc cgcctgaatg gcgcgattga attcgctttc tttaaaatcg
     1021 taaccgccaa ccatgctaat cacatcgcca gatcttacat ccagcgaaat cagtgcgccc
     1081 tgtacaaggg gtacctgctc taatgctaaa gacaagtatt cgggccgatc tttcatttct
     1141 ttagccaaca gcgaaaccca aataacgtcc ccccgagata aggcctgcga cggaaaattt
     1201 attgcctcta ctgcgctcgc gccacttcga ttcggattag cccaccgcat atcctcaatt
     1261 aaaatgattc ccttggtcac acctagatca acgagaactt ctttcttttt gtcgtcgact
     1321 tgtacaacca acccttgata aagctcgtct tcctgaaaag cgtcggctgg tttactaagc
     1381 tgcgcagaat aatactttat atcggtgctt agtacaccct catccaccaa cacagcatac
     1441 ggaaactttt tttcgagatt aaaatgttga atctccgcaa tagcgctggc ctcttcggag
     1501 cgaattttga tttttttaat tggaccgcgg taaccaagcc tgcgatccac tgttctcacc
     1561 cctcgtctca ccgctttggt tccgacttca gcaagtctag catctatggg cgagagtacc
     1621 tcgagccctt cttcgtaaag cttttccttt ccgtattttt cgagcaaata ctttctcacg
     1681 tgctcgacat aatacggtga atatttcaga ttgtagtcct gagtgtagta tagattgacc
     1741 ggttgaacgc tcgcctcttc catttgccgc acagatatcg cccccgactc catcatgcgg
     1801 cgcagaacat acagctgtcg ctgttttgct gattttggat tttgtaaagg tgagtattta
     1861 cttggtgcct gcggaagccc tcctaacagt gcactttcag ccaaagttaa gtcttttgca
     1921 tttttattga aataaattcg cgcggccgcc tctatgccat aggctccctg gcctagataa
     1981 atttgattta agtaaagata caatatttgt tttttattaa aatttcgctc cattctgttt
     2041 gcaagaataa gctccttaaa cttgcgaaca aaacttcgct caggggtaag taataaactt
     2101 ttagctactt gttgcgttat cgtacttcca ccttgtacga cacgtccggc cttaaaattt
     2161 gcgatcgccg cccgtaaaat tccatggaag tcgattccct ggtgctcaaa aaagcgatcg
     2221 tcttcagcag cgacgaaggc ctttacggct aactctggaa tttgatctgg aggtaaaacg
     2281 tatcgtcgct cttcaaagtg ttcaccaata cccgtgccat cttttgctcg aatggtagtt
     2341 acgattctcg gtttgtaatc ttcaagtgtg aataacgcgg gcagatcgct agaaaaatag
     2401 agaaacactg ctccaccgac cacacaaatc gccaatgttg aaagcactaa gccaataaat
     2461 ccgaatctga gaatcgacag caatattttt ggtgtcttgc cgttatcgat tttttcggat
     2521 ccgttattgc tttcttctac ttcttggtcg ccagccactt ttgaacgcct gcctttcctc
     2581 gaaatatgag ttctgccttc tgttcaaaat cgaaatatcc aattccgtca gtggggatgc
     2641 gaacaacttc atcgcaagct ttaagctgtt ctttcgataa cgcagccact gttctcatct
     2701 gagctgcaat ttggtgttca taattttcct tcggcgtaaa gttattacct cgtcccacca
     2761 cgtcaatgca gagggttttg ccatacccta gatctcgggc ataatcgctt ggaaatggaa
     2821 tctcgaacgc agcgttggat cttgttaatc cgccgacctt tatggtctca atgatgttag
     2881 gcaaacctaa tgcaccccta aggatttcga tcggattccc ttgccgttcg aaaatcggac
     2941 cgttttcagt cgaagaaact acaattatag gaatcttaga aaccgcgagg tcttttactg
     3001 atatt
//
LOCUS       DAOMXO010000253         3002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18490_length_3002_cov_3.974550, whole genome shotgun sequence.
ACCESSION   DAOMXO010000253 DAOMXO010000000
VERSION     DAOMXO010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18490_length_3002_cov_3.974550"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLH57_06150"
     CDS             <1..725
                     /locus_tag="PLH57_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRK02230.1"
                     /translation="FTSPIAYLVMAIFSAIMGYMFFSLLNLYVLQSMQFEQLRYGKGP
                     SFNDSLIRPIFGNMNVVLLFIVPFITMRLFAEEKKNNTIELLFTAPIKMSQIVLGKYL
                     SALLFVLVLLLLTIPYPIALSVAANPDWGVIAMCYLGTILMIGTYIAVGVLCSSITEN
                     QIVAGALTFGTILFFWIIKWASYNASPFWSDLLSYLSIVDHFEDFSRGIFNTRDIVFF
                     ASSIGLWLFLTYKTLESYSWRS"
     gene            729..2297
                     /locus_tag="PLH57_06155"
     CDS             729..2297
                     /locus_tag="PLH57_06155"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HRK02231.1"
                     /translation="MKGNKQSGDILQKVLGIATGFFALAFLAVVFIYPEIKSAVLTFA
                     VLLLACLGALAWRNQKSLKSAFAQRSTRYGANAALTVGLVFAIVVVLNFLNFNHHWKK
                     DLTENKIHSISDQTKKALEGLQEDLNITVFVKAQERERAKSFFGNYTYLTKKVKVEYV
                     DPDRDPQRAKALNVKKYNTAIVALGKKDTRVDDYTEEKVTNAIMKLMKTGVTRFCLVT
                     GHGEKLLDNPGPDGLGQLKQELESQNFEVQSLNLLEEGKIPEGCTVVAVVGPEKAFFE
                     KEIELIDAWLKLGGRLVLALDPPVRSNSVVQKELIGLADKWAIEFRHNLIVDPTSKLL
                     GGNASVPLVGTYNKDHPIVKDFQIATLFPLPSGVDVKSTVPGMKTWWVAKTTQRSIAK
                     SDFKEIATGRVKVNEKTDKVEAHAVMAAVEGRAAGTKPDARPARIVAFGTSNLVNNAY
                     ARHGANMDLFMNSLRWLADEESFISIKSREDGPVIPSLSQEQGRAIQFATMFLVPGGS
                     LLWGLAIWLRRRKL"
     gene            2258..>3002
                     /locus_tag="PLH57_06160"
     CDS             2258..>3002
                     /locus_tag="PLH57_06160"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRK02232.1"
                     /translation="MGSSDLASPKKTLSSSLKTNLILAGLLAALTGAVYWYEFYSKPV
                     REKTKSNSNKILPIGDEMELDRLVIKNETKGIEVELQCRQNCKFNDPKADWTLNKPLS
                     FDADDPNVGTFLAATFGADVIETLNFEGEEKPNLESFGLAENQKKNLAQLYVKGRSEP
                     MEVATGDNTAIGGSVYAHTSDGRLLIVNHAVRVNLERDQTYWRNKKLFNFDGNKVIAL
                     KLVNKSGTIELKKIGDQWSFRDGKPAEYDT"
BASE COUNT          829 a          614 c          724 g          835 t
ORIGIN      
        1 attttacttc gccaatcgcg tatctggtta tggcgatctt ttcggccatc atgggataca
       61 tgtttttttc gctgctcaat ttatatgttc ttcagagcat gcagttcgaa caattgcggt
      121 acggtaaggg ccccagtttt aacgacagcc tcatacgacc aatatttggc aatatgaacg
      181 ttgtgctcct ttttattgta cccttcataa cgatgcggct tttcgcagaa gaaaagaaga
      241 acaacacaat cgaacttttg ttcactgccc ctataaaaat gagccaaatt gtacttggaa
      301 aatacctttc ggcattgctt tttgttttgg tacttttact tttgaccatc ccttatccga
      361 tcgccctttc cgttgcagct aatcccgatt ggggagtgat cgccatgtgc tatcttggaa
      421 cgattttgat gataggcaca tatatcgcag tgggagttct atgttcctcg atcacagaaa
      481 atcaaattgt cgcgggcgca cttactttcg gaaccatttt atttttttgg atcattaaat
      541 gggcctcgta caatgcaagt ccgttttgga gtgatttatt aagttatctt tctatagttg
      601 atcacttcga agatttttct cgtggcattt ttaatacgcg agacatcgtt ttcttcgcct
      661 cgagcatagg gttgtggttg tttttaactt acaaaacgct cgagtcgtat agttggaggt
      721 cgtaatccat gaagggcaat aagcaatcgg gtgatattct ccaaaaagta cttggaattg
      781 ctactggctt tttcgcacta gcatttctgg cggtggtttt tatttacccg gaaattaagt
      841 ctgcggtctt gacgtttgct gttcttttgt tggcgtgcct tggtgcgctg gcctggcgta
      901 accaaaaaag tttgaaaagt gcctttgcgc aacgatcgac gagatacggc gcgaatgctg
      961 cgcttactgt ggggttagta tttgcgattg tggtagtcct taattttctt aattttaacc
     1021 atcattggaa aaaagacctc actgaaaaca aaattcactc gataagcgat caaaccaaaa
     1081 aagctcttga agggttacaa gaggacttga acataactgt gtttgtaaaa gcgcaggagc
     1141 gagagcgagc taagtctttt tttggtaatt acacctacct cacaaaaaaa gtgaaggtag
     1201 agtacgtcga tcctgaccgc gatccgcaac gggcgaaagc gttgaacgta aagaaataca
     1261 ataccgcgat cgtcgcgctt gggaaaaaag atacgcgggt ggatgattat actgaagaaa
     1321 aagtcaccaa cgcaattatg aagcttatga aaacgggtgt aactcgtttt tgtttggtaa
     1381 ctggtcacgg ggaaaagttg cttgataacc ccggcccgga cggacttgga caactcaaac
     1441 aagaactcga atctcagaat ttcgaggtac aaagtttaaa cttgctcgaa gaaggaaaaa
     1501 ttcctgaagg ttgcacagtc gttgcagttg ttggtcccga aaaagcattt tttgaaaaag
     1561 agattgaact gatcgatgct tggctgaaac tcggtgggcg ccttgttttg gcgttagacc
     1621 cacccgttcg atcaaattca gtggttcaga aggaactcat cgggctcgca gataaatggg
     1681 ccatcgaatt tcgacacaat ttgatcgtag atccgaccag taaacttctg ggcggcaacg
     1741 ccagtgtacc tttggttggc acctataata aggatcatcc catcgtgaaa gattttcaga
     1801 ttgccacttt atttcctctt ccgtctggcg ttgacgtaaa aagtacagtg ccgggaatga
     1861 agacctggtg ggtggcaaaa accactcagc gttccattgc gaaatcggat tttaaagaaa
     1921 ttgcaactgg tcgggtaaaa gtgaatgaaa aaacagataa agtcgaagct cacgcagtaa
     1981 tggcagccgt cgaggggcgg gccgcgggta caaaacccga tgctcgaccg gcccgcatag
     2041 tggcgtttgg cacatctaat cttgtgaaca atgcttatgc gcggcacgga gctaacatgg
     2101 atttatttat gaattcactt cgttggttgg ccgacgagga atcttttatt tcaattaaat
     2161 ctcgtgaaga tggtccggtc attccgtctc tttctcaaga gcagggccga gcgattcaat
     2221 ttgcgacgat gttcttagta cctggaggtt ccttattatg gggtctagcg atttggcttc
     2281 gccgaagaaa actttgagct cttcgctaaa gacgaactta atattggcag gactgctggc
     2341 cgctcttacg ggcgccgtct attggtacga gttttattcg aagccagtac gggagaaaac
     2401 caaaagtaat tccaataaaa tcttaccaat cggcgatgaa atggaactcg atcgtttggt
     2461 gataaaaaat gagaccaaag gtatcgaagt tgaactgcag tgtcgccaga attgcaaatt
     2521 taatgaccca aaggctgact ggactctaaa caaaccactt agcttcgatg cagatgatcc
     2581 gaacgtcggt acttttcttg ctgctacttt tggtgctgat gtgatcgaga cactgaattt
     2641 tgagggtgaa gaaaagccaa atttggagtc gttcgggttg gcggagaacc aaaaaaagaa
     2701 tctcgcacaa ctttatgtaa agggccgctc ggagccgatg gaggttgcca cgggcgacaa
     2761 taccgctatt ggtggaagcg tttatgcgca taccagtgac gggcgactat tgatcgtcaa
     2821 tcatgcagtg cgagttaact tagagcgaga tcaaacttac tggcgcaaca agaagctctt
     2881 caattttgat ggcaacaaag tgatagctct caagctcgta aacaaaagtg gcacgatcga
     2941 acttaaaaaa ataggggatc agtggtcgtt tcgagacggg aagcctgccg aatacgacac
     3001 ag
//
LOCUS       DAOMXO010000254         2995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18550_length_2995_cov_4.193197, whole genome shotgun sequence.
ACCESSION   DAOMXO010000254 DAOMXO010000000
VERSION     DAOMXO010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18550_length_2995_cov_4.193197"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PLH57_06165"
     CDS             complement(<1..1127)
                     /locus_tag="PLH57_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HRK02233.1"
                     /translation="MVRFWVVFIFFLINATFASANLVVQIHLDGVINNGTTKYVVRAV
                     EYAQQKNADFLAIRLNTPGGLLNSTRDIVSAMNASKVPIGVFIGPGGASATSAGTIIA
                     MAANVSGMAPGTNIGAAHPVAGQGQDIKGDMGEKAVNDTVAFIQSQAQLRGRNVQWAE
                     QAIRKSASLAANDAVAEKVVDFVADDFDSFVRKAAEKIGKPFQMVPIEHLEMTKAERV
                     LHFLGDPNVSYMLMVLGGVGLYAEMSSPGLVFPGVVGALCLVLALISFSTLPVNTGAV
                     ALLILAVVFFILEAFITSYGLLAVAGIVSMLLGGLFLMDSSTGDLRLSLALLIPVVLA
                     FGSIMLIIGYMFRRESRGRASLELEGQVAEIVALSPSAAHST"
     gene            complement(1131..1931)
                     /locus_tag="PLH57_06170"
     CDS             complement(1131..1931)
                     /locus_tag="PLH57_06170"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRK02234.1"
                     /translation="MTEKNAHLSQQQKEQMKEHLKRTYGEDAIVSKRALTRRIAGASK
                     ARQFSSTALRPTVAAPPLASVGNAVDALPIDLKQPALNETLQEIKKDLGDCTRCKLCK
                     TRTNIVFGEGNEKAKIMFVGEGPGETEDLNARPFVGRAGQLLDKIIEAMGYKREDVYI
                     ANVVKCRPPGNRVPEPQEVATCEPFLFRQIDVIKPSVVVALGGTALQSLLHSDAKISK
                     MRGKFVDYRGTKLMPTYHPAFLLRNPSAKKDVWDDMKVVLELLKKESA"
     gene            2071..>2995
                     /locus_tag="PLH57_06175"
     CDS             2071..>2995
                     /locus_tag="PLH57_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02235.1"
                     /translation="MILTDSLQPHEYSPVNTLVEVLHREFGKLRIKISSRSLERVSVM
                     LYEAMSTRNRAYHRIEHSLNYPKNLSPIAVIAYLFHDIVYWQIDRARHQALRQYMSPY
                     APTEDGVSELFDALDLNNIDLNEPGILSRLVFGFENRTQITPAEGLNEFLSACVAENV
                     FKGILKPWQMIHLLACIEATIPFQKPVGDVTAPMALRARLKNIATALKLKVSDTDLRA
                     VTTEAIELALHDLNNFAAGDSGLFLKNSWEVIWESNPGLQKETYTVKQFRKAIQQTEA
                     FFSSIDLRLIFPFTLDYPDHRVLTHLWEATEH"
BASE COUNT          750 a          783 c          708 g          754 t
ORIGIN      
        1 gtactatggg cggcagacgg gctgagcgca acaatttcgg ctacctgccc ttcgagttcg
       61 agtgaggccc tccccctact ctctctgcga aacatatacc cgataatgag catgatgctg
      121 ccaaaagcta aaaccacggg tatgagtagc gcgagcgaaa ggcgaagatc tccggttgaa
      181 gaatccatca ggaatagccc gcccaaaagc atggaaacga ttccagctac cgcgagtaaa
      241 ccgtaactcg taatgaaagc ttcaagtata aaaaacacga ctgctaaaat gagtagcgcc
      301 actgcaccgg tgttcactgg caaagtggaa aatgaaataa gtgcgagcac cagacacaaa
      361 gccccgacca caccaggaaa caccagaccc ggcgaactca tttcagcgta aagaccgact
      421 ccccccaaaa ccatgagcat ataactaacg ttgggatcgc ctaaaaaatg caaaacacgt
      481 tcggctttgg tcatttcgag gtgttcaatt ggaaccattt gaaatggttt tccaattttc
      541 tctgccgctt ttcgaacaaa ggagtcgaag tcgtccgcta caaaatcgac cactttctct
      601 gccactgcgt cgtttgcagc gagcgatgcc gattttctaa tcgcttgttc ggcccactgc
      661 acgttgcgcc ctcgtaactg cgcttgggat tgaatgaacg ccacggtgtc gttgacggct
      721 ttttcaccca tgtcgccttt gatgtcttgg ccttgcccgg ccacggggtg tgcagctccg
      781 atattggtgc caggcgccat accactcaca tttgctgcca tcgcgatgat ggtgccggcg
      841 ctagtggctg aggctccgcc gggcccaata aaaacaccga tcggaacctt gctcgcgttc
      901 atggcgctaa caatgtcgcg agtgctgttg agtagtccac caggagtatt taagcgaata
      961 gccaaaaaat ctgcattctt ttgctgcgcg tattcgacgg cacgtactac gtatttggta
     1021 gttccgttat taattacgcc gtcgaggtgg atttgaacca cgaggttggc gctggcgaat
     1081 gtggcgttaa tgagaaaaaa aatgaagacc acccaaaaac gcaccatttt ttacgccgac
     1141 tctttcttta gtagttcgag cacgactttc atgtcgtccc aaacgtcttt tttggctgaa
     1201 gggtttctta acagaaatgc tgggtgatag gtgggcatga gtttggtacc gcgatagtcc
     1261 acgaacttgc cccgcatttt actaatttta gcatcgctat gtagaagcga ctgtaaggcg
     1321 gttccaccga gcgcaacgac cacacttggt ttgatgacgt cgatttggcg aaacaaaaaa
     1381 ggttcgcagg tggccacttc ttgtggttca ggcacgcggt tgcccggcgg tcggcacttc
     1441 accacattgg cgatataaac gtcttcccgt ttgtagccca tggcttcaat gattttgtcg
     1501 agcaattgac cggcgcggcc cacaaatggg cgggcgttga gatcctcggt ttcgccgggg
     1561 ccttcgccga cgaacatgat ctttgctttt tcgtttcctt cgccaaaaac aatattagtg
     1621 cgcgttttac aaagtttgca gcgcgtacaa tcgccgagat ccttttttat ttcttggagc
     1681 gtttcgttca gcgctggttg tttgaggtcg atcggtaacg cgtcgactgc gttgccaacc
     1741 gaagcaagcg gcggtgccgc gacggtcggg cgaagcgctg tgcttgaaaa ctgacgagcc
     1801 ttcgaagctc cagcaatgcg acgagtcaat gctcgttttg agacgatcgc gtcttcgcca
     1861 taggttcttt ttaaatgctc tttcatttgt tctttctgct gttgcgaaag gtgggcattt
     1921 ttttccgtca tcgtacgcag cctcgtgttg gtggtttcaa cttttctctc tgcaaagtac
     1981 tccggtgtca agctgttcag cgggctttag cgaacactat cttttaccga attgtataaa
     2041 gacacgaata accaaaggac agcgaacgca atgattctca ccgactcgct ccaaccgcac
     2101 gaatattcgc cagtcaacac actggtcgaa gtgctgcacc gcgaattcgg gaaattacga
     2161 ataaaaatca gttctcggag cctggaacgc gtttcggtca tgctgtacga agccatgtcc
     2221 acccgtaatc gcgcctacca ccgcattgaa cattcgttga attacccgaa aaatctcagc
     2281 cccatcgcgg tgattgccta tttattccac gacatcgttt actggcagat cgaccgcgcc
     2341 cgccaccaag ccttacggca gtatatgtcg ccctatgctc ctaccgaaga cggggtctcc
     2401 gaacttttcg atgcattaga cctaaacaat attgatctaa acgagcccgg catactttca
     2461 cgtttagtgt tcgggttcga aaaccgcaca cagatcacac cggccgaagg tctcaacgag
     2521 ttcttaagcg cctgtgtggc cgaaaatgta tttaaaggca ttttaaaacc atggcaaatg
     2581 atccacctac tcgcgtgcat tgaggcgaca attccttttc aaaaaccagt gggcgacgtg
     2641 actgcaccca tggcccttcg tgcccgtctt aaaaatattg cgaccgctct aaagctcaaa
     2701 gtttccgata cggatttacg cgcagtcact accgaagcta ttgaactagc tctgcacgat
     2761 ctcaataatt tcgcagcggg agatagtgga ttatttctta aaaattcgtg ggaagtcatt
     2821 tgggaatcga accccggact tcaaaaagaa acgtacaccg tcaaacaatt tcgaaaagca
     2881 attcaacaaa ccgaagcttt tttttcttca atagacctgc gtctgatttt tccatttaca
     2941 ttagactatc cggaccaccg agtcttaacc cacttgtggg aggccacgga acaca
//
LOCUS       DAOMXO010000255         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18601_length_2990_cov_4.481431, whole genome shotgun sequence.
ACCESSION   DAOMXO010000255 DAOMXO010000000
VERSION     DAOMXO010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18601_length_2990_cov_4.481431"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PLH57_06180"
     CDS             complement(<1..1128)
                     /locus_tag="PLH57_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HRK02236.1"
                     /translation="MLNWDELKHIHVIRKLEEILGDWFYTDIFFVDEKGEFQNWVPGE
                     KKEFRNLLTSVLVSKPGGYQSLIQFGAQIVEKNIRSDKSTQLIDGIFGFEKVMAARIA
                     VDSEFLGCVVAMPVLGHDILSADKTKAKELAKTLGLDAGDMDKAVNGIKAISTADMEY
                     FRQLVELVAQEIVTFHTEISRREERINALNQELGTRYRYDSMIGKSKPMQDLYALLDK
                     IKASESTVMIQGENGTGKELIAKAIHYNSPRKDAAFVTVNCSAFNENLLDSELFGHVK
                     GAFTGAVKDKKGLFESADKGTLFLDEIGDMSPSMQVKLLRVLQEGTFIPVGGTEARKV
                     DVRVLAATNKDLKEMIETGAFREDLYYRVNVINITVPPLRDR"
     gene            complement(1230..2171)
                     /locus_tag="PLH57_06185"
     CDS             complement(1230..2171)
                     /locus_tag="PLH57_06185"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRK02237.1"
                     /translation="MKTLTGVIEIVNPRSGNGRAQRLADTSELKKKVYDSILNNTNGN
                     ARHKIETDLEGRWLKELVSVAKSQPDALLAVFGGDGTLSLVAQTVLAHALATKLLFIP
                     GGRGNDFTRCLFGREFQEKDFLDWAVQKHWQASPIDLGQCNERVFLNMASIGYGGSVV
                     ENVHERNAFWSKSPAVYQVEGALALLAEPEVSVAVDEGDSTKKIYIGPIFGAFVGNGC
                     ANGSGLYWVPEARCDDGKLDLFLLRKPGFFSMLKALKEVKLKQKLSCDHQIATAKTFR
                     LQFEKPVALELDGEYCGRHQSFEIKTVARAIEVYRPI"
     gene            complement(2177..>2990)
                     /locus_tag="PLH57_06190"
     CDS             complement(2177..>2990)
                     /locus_tag="PLH57_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02238.1"
                     /translation="FETASSDPKLMKEVAVSVGKDGTGLRKTLNEASRLRTDVADRWL
                     HQADPKYRAALDGALHTLVESRKKKAVPVLPETDLSNIEVDDIVVVTHQQPGLQQATA
                     EFLAKNKLLKKAPRVALVSPDYRVVTEDFVNQLSTARYSSDGRAPKLPASRRVHLMGG
                     NLEECFSKTIDDMIAQYIFKNEERSGDLQFFFYRDLIYSGPKTLREILKEKSKFTDLS
                     DLIEAELLMPDSLDALGLVPYTPPLKEKGKKSGLDFFYIHKTSGKIIHFHIL"
BASE COUNT          758 a          756 c          624 g          852 t
ORIGIN      
        1 tcgatcgcgc agagggggga ccgtgatatt gattacgtta acccgatagt acaggtcttc
       61 acgaaacgcg ccggtttcga tcatttcttt aagatccttg ttggttgctg ccaaaacccg
      121 aacgtccacc tttcgggcct cggtgccgcc tacgggaata aaagtgcctt cctgcaaaac
      181 ccgtaacagt ttgacctgca tgctgggcga catgtcacct atttcatcta aaaacaatgt
      241 gcccttgtcg gcactctcaa ataggccttt tttgtctttt accgcgccag taaaagcccc
      301 ttttacgtgg ccaaaaagtt cgctgtcgag gagattttca ttgaaggcgg agcaattcac
      361 agtcacgaaa gccgcgtctt ttcgtggact attgtagtga atcgcctttg cgataagttc
      421 tttaccagtt ccgttttcac cttggatcat cacagtgctc tcacttgctt taatcttgtc
      481 tagcaaagca taaagatcct gcatcggctt cgatttgccg atcatgctgt cgtagcgata
      541 acgcgtaccc aactcctgat tcaacgcatt tattcgctct tcgcgtctcg atatctcagt
      601 atgaaaagta acaatttctt gcgcaacgag ttcaaccaac tgccgaaaat actccatatc
      661 tgcagtagat atcgctttga tcccattaac agccttgtcc atgtcaccag cgtcgagacc
      721 tagagttttc gctaactctt ttgctttggt cttatcggcc gaaagaatat cgtgtccgag
      781 aaccggcatc gctacaacgc agcccaaaaa ctcagaatcc accgcaattc gcgcggccat
      841 tactttttca aatccaaaaa tgccgtcaat gagttgggtt gatttatccg aacgtatatt
      901 tttttcgact atctgcgcac cgaattgaat caaggactgg tacccgcctg gtttagaaac
      961 caagaccgaa gtcagcagat ttcgaaattc tttcttttca ccaggaaccc agttctgaaa
     1021 ctcgcctttt tcatcgacga aaaaaatatc ggtataaaac caatcaccaa gaatttcttc
     1081 gagttttcga attacgtgaa tgtgttttaa ttcatcccag ttgagcatgg cgccctctcc
     1141 caatgtcctt aaaattccat tctatgttta atttatcgac actgcctgat taaagtttaa
     1201 tcagtgggaa aaattttcct aaaaccctac taaatggggc gataaacttc gattgcacgc
     1261 gcgacggttt taatctcaaa actttgatga cgcccgcaat attcaccgtc gagttctagt
     1321 gcgactggtt tctcaaactg taaccgaaat gtttttgcgg tagcgatctg gtggtcacag
     1381 cttagcttct gtttgagttt cacttccttg agcgctttta gcatggaaaa gaagccaggt
     1441 tttctgagca aaaacaaatc aagtttacca tcatcacagc gagcctcagg aacccaataa
     1501 agtccactcc cgtttgcaca accattacca acgaacgcac caaatatggg tccaatatat
     1561 attttttttg tcgaatcacc ttcgtcaact gcgaccgaaa cttcgggttc agccaaaagc
     1621 gccaatgccc cttccacctg ataaacggct ggggacttgg accaaaaagc gtttcgctcg
     1681 tgaacatttt ctactactga accgccatac ccaatactcg ccatattcag aaatacgcgt
     1741 tcgttgcact ggccgagatc gatgggactc gcctgccaat gtttttgaac ggcccagtct
     1801 aaaaagtcct tttcctgaaa ctcacgccca aataaacagc gtgtaaaatc atttcctcga
     1861 ccgcctggaa tgaatagaag tttagtcgct aaagcatggg caagcaccgt ctgcgctacc
     1921 aacgataacg ttccgtcgcc gccaaatact gcaagaagtg catcgggctg cgatttagca
     1981 acagagacta gttcttttaa ccagcgtccc tcgaggtcag tctcgatttt gtgccgagca
     2041 ttgccgttcg tattattcag aattgaatcg taaacctttt tcttcagttc actcgtatcg
     2101 gcgagtcgct gggctcgacc gttcccactg cgcggattta cgatttcaat gactcccgtc
     2161 aaagtcttca tggacgctac agtatgtgaa agtgaatgat ctttccagac gtcttatgga
     2221 tgtaaaaaaa atctaaccca ctctttttac ccttttcttt taaaggtggg gtgtacggca
     2281 ctagacctag ggcatctagt gaatccggca tcagaagttc ggcctcaatt aaatcactta
     2341 ggtccgtaaa cttggatttt tcttttaaaa tttctctcaa cgttttcggt cccgaataaa
     2401 taaggtcgcg atagaagaaa aactgcaagt cgccgctccg ttcctcattt ttgaatatgt
     2461 actgagcgat catgtcatct atcgtcttcg aaaagcattc ttcgagattg ccgcccatta
     2521 ggtgaactcg tcggctagcg ggcagttttg gtgcacgtcc gtccgacgag tatcgagcag
     2581 tgctcaattg gtttacgaag tcttcagtaa caacgcggta gtcgggcgat accagcgcga
     2641 ctctaggtgc cttttttaat agtttattct tcgccaggaa ctcggcagta gcttgttgaa
     2701 gtcccggctg ttggtgggtg acaacgacaa tatcgtcgac ttcaatattt gaaaggtcag
     2761 tctccggcag aacaggaacc gccttttttt ttcgcgactc cactagtgta tgaagcgctc
     2821 cgtctaaagc agcgcgatat ttcggatccg cttggtgaag ccagcgatcg gccacatcgg
     2881 tgcgtagtct actggcctcg ttcaacgttt ttcgaagtcc agtgccgtct ttacctacgc
     2941 taaccgctac ttctttcata agttttgggt cggagctcgc ggtttcgaaa
//
LOCUS       DAOMXO010000256         2987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18631_length_2987_cov_5.726808, whole genome shotgun sequence.
ACCESSION   DAOMXO010000256 DAOMXO010000000
VERSION     DAOMXO010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18631_length_2987_cov_5.726808"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /gene="feoB"
                     /locus_tag="PLH57_06195"
     CDS             <1..1623
                     /gene="feoB"
                     /locus_tag="PLH57_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRK02239.1"
                     /translation="DSTNLEVNLYLVLQLIERKTPLLLVLTMSDLAQKQGVQVNVEKL
                     EQILGVPVVAVSARKKQGLAPLIKILSQKIDTPSTATDVPASTATPRSVSEDILARYV
                     RIESILKSCVQHTETLHSQFTRKLDRILLNRFAGPIILLAVLAFMFQSIFSFAEFPMD
                     LIESGVAFTGDFVRTLLPEGMLQSLLVDGVIAGVGSVVVFLPQILILFAFILVLEDSG
                     YMARATFILDRLMAAIGLNGRAFIPLLSSFACAIPGIMATRTIESRRDRFITILISPL
                     MTCSARLPVYALLIAAFIPSTTVWGFVGLQGLVLLGLYLGAILFAVIVAAVLRKTLYR
                     GENSKFVMELPTYKRPSLRNLCVGLLDRAKVFLKRAGTVILVTSIVLWVLATFPKVEA
                     PQEVAMQGEAAIASYQLQNSYAGLAGHAIEPLIAPLGFDWRIGIGLISSFAAREVIVS
                     TLSTVFAVQGGDSAETELGTRLAADPVFTLPTVLALLVFFMLACQCISTLAVVKRETN
                     SWTWPMAMLAYMTTLAYTAAFLTREAAMAIGL"
     gene            1687..>2987
                     /locus_tag="PLH57_06200"
     CDS             1687..>2987
                     /locus_tag="PLH57_06200"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRK02240.1"
                     /translation="MNLLKRLFRITPFKLSLSITLVFLTGSLIHDFNPSKVPFIQAID
                     QKSLDLKFHLRGAQASPSKIVILAADEKAFQTFGQWPFDRGTIFAPMLRELCKLNTKV
                     VGLDLVWTDREKLIAPKLKSQLAERLGASAADLDGMIAGSSGDQQIREAVEDCKNKIV
                     LGYAPTRNASDGLSETELKKRVEILTTQGGNAMLSRRTGRLKFARFRADREANVSFSY
                     NANGGQFNTADITPFGVAQGFMNNEDDGDGSYRHGLMFFRVADTFVPSLSLRMAQKFL
                     SPNDEPPGISIVPNAPEQGMTESLTLTIKTAEGPRTVPIDLHAQTLVNYRGPNYTYPN
                     VSLADLLSGKDKIPYVQYIPGVGAQNFEGSKKDLFDQALVLVGVTAMSLYDIRPRPLE
                     EIAAGVENHANILDNLINDDFLKRPSAENFLIVMMAIFVASL"
BASE COUNT          744 a          755 c          681 g          807 t
ORIGIN      
        1 gacagcacca atctggaggt aaacctctat ctcgtgctgc aattgatcga acgcaaaact
       61 cccctattat tagtattaac aatgtctgac ctcgctcaaa aacagggcgt tcaagttaac
      121 gtcgaaaagt tagaacaaat tttaggtgta cctgtggtcg cggtatctgc aagaaaaaaa
      181 cagggcctgg ctcctttgat taaaattctc tcgcaaaaaa tagatactcc ttcgactgcc
      241 accgatgtac cggcatcgac tgcgacaccg cgctcggttt ctgaagacat tttggcacgg
      301 tacgtacgca ttgaatcgat tttaaaatct tgtgtacaac acaccgaaac tctccacagc
      361 cagttcaccc gaaaactcga ccgcatctta cttaaccgct ttgcggggcc cattattttg
      421 ctcgctgtgc tggccttcat gtttcaatcg atattttcgt tcgcggaatt tcccatggat
      481 cttatcgaat cgggtgttgc tttcacaggc gatttcgtac gaacccttct gcccgaagga
      541 atgcttcaat cccttctggt cgacggagtg atcgccgggg tcggatcagt cgtggttttt
      601 ttaccgcaaa ttctaatatt attcgctttc atcttagtac tggaagattc aggttacatg
      661 gctcgggcga cttttattct ggatcgttta atggctgcaa tcggtttaaa cggtcgagcc
      721 tttattccgc tgcttagctc ctttgcctgc gcgattccgg gcataatggc gactcgaacg
      781 atcgaaagtc ggcgagaccg ttttataacc attctcattt cgccattaat gacctgttcg
      841 gcgcgactac ctgtttacgc gttgctcatt gccgccttca ttccgtcgac aaccgtgtgg
      901 ggtttcgttg gtcttcaagg tctagtgcta cttggacttt acctcggcgc tattttgttc
      961 gcagtcatcg tggctgcagt gttgcgaaaa acactttatc gcggtgaaaa ttccaagttc
     1021 gttatggaac ttccaactta caaacgacca tctcttcgca atttgtgtgt tggtctattg
     1081 gatcgtgcga aagttttcct aaaacgggcg ggaaccgtaa tactcgtaac ctcgatcgta
     1141 ctttgggttt tggctacttt tccaaaggtc gaagcaccgc aagaggtagc tatgcaaggc
     1201 gaagcagcga tcgcttctta tcaattacaa aatagctatg ccggtttagc gggtcatgca
     1261 attgagcctc taatagctcc gcttggattt gactggcgaa tcggtattgg ccttatttct
     1321 tcattcgccg cccgtgaagt aatcgtcagc acccttagta ccgtatttgc cgttcaaggt
     1381 ggtgacagcg cggaaactga acttggtacc cgacttgcgg cggaccccgt attcacgctc
     1441 ccaacggttc tcgcactttt ggtgtttttc atgctcgcat gccagtgtat ttctaccttg
     1501 gccgtcgtaa aacgagaaac caactcctgg acttggccca tggcgatgct ggcttacatg
     1561 accacactcg cctacaccgc tgcattttta actcgcgaag ctgcgatggc catcggtctt
     1621 taactcatcc aataggtaga atttaccggt cctcacgaaa ctgccggcaa ccaccgatag
     1681 gtttgcgtga acttgctgaa aaggctgttt cgcatcacgc cattcaaact ctcgctttct
     1741 attacgctgg tatttctcac cggttcgttg attcatgatt ttaatccctc gaaggtgccg
     1801 tttattcagg cgattgacca aaaaagcttg gatcttaaat ttcaccttcg cggcgcccaa
     1861 gcatcacctt cgaaaatcgt gattctagct gccgatgaaa aagcatttca gacttttggc
     1921 cagtggcctt tcgatcgcgg aaccatcttt gcgccaatgc tgcgtgaact atgtaaattg
     1981 aataccaaag tagtgggact cgacttggtc tggactgatc gggaaaaatt aattgctccg
     2041 aagttaaaat cacaactagc agaaagactt ggagcaagcg ctgccgatct cgacggaatg
     2101 atcgcgggtt ccagcggtga ccaacaaatt cgagaagcgg tagaagattg taaaaacaaa
     2161 atcgtactcg gttatgcacc cactcgcaac gcgtctgatg gcttaagtga aacagagtta
     2221 aaaaaacgag tagaaatcct caccactcaa ggcggcaatg caatgttatc gcgccgtacc
     2281 gggcgtttga aatttgcacg gtttcgggct gatcgtgaag caaacgtttc attttcgtat
     2341 aacgcaaacg gcggtcaatt taacactgcc gacatcacgc cctttggtgt cgcacaaggg
     2401 ttcatgaaca atgaagacga cggcgacggt agctaccggc acggtctaat gttttttcgg
     2461 gtggccgaca cgttcgttcc aagcctgagc ttgcgaatgg cccagaagtt tttgagtcca
     2521 aacgacgaac cacctggcat ttcaatcgta cccaacgctc ccgaacaagg aatgaccgaa
     2581 tcgctcacgc tgaccattaa aactgccgaa ggaccgcgca cagtcccaat cgatctacat
     2641 gcacaaactc tagtgaacta tcgcggcccc aattatacct acccgaacgt gagcctagct
     2701 gacttacttt caggcaaaga taagatccca tacgttcaat acattcccgg cgtgggagcc
     2761 cagaactttg aaggttcaaa aaaggacctc ttcgaccaag cgcttgtact agtgggcgta
     2821 actgccatga gtctttacga tatacgccct cggccgctcg aagaaattgc tgcgggggtg
     2881 gaaaatcacg cgaatatttt agacaatttg attaacgacg atttcttaaa acggccgtct
     2941 gctgaaaact ttctcatcgt aatgatggca atatttgtgg cgagtct
//
LOCUS       DAOMXO010000257         2986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18646_length_2986_cov_5.119072, whole genome shotgun sequence.
ACCESSION   DAOMXO010000257 DAOMXO010000000
VERSION     DAOMXO010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18646_length_2986_cov_5.119072"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLH57_06205"
     CDS             complement(<1..1126)
                     /locus_tag="PLH57_06205"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRK02241.1"
                     /translation="MLFDNLLSTLTLLPIVGAAIICLIPSGDRPIHRLVASVFALGAL
                     LLSVVCLVEFDFSSNTLQFVSQHRWVSSLNISYNVGLDGLNVFLVCLVALIFFLSIFA
                     AKQFRYIKGGAASFSESGYQISGAKGYFALFLLLQSTVFGMCLAQDLFLFLVFFLMAS
                     VPLYFLIGVWGAKDRERAATEYVVYQLIGAVFILMGMLLLYYTAEPHTFDLGALNDQK
                     LNGTKILFLDREWDLEKSAYLLMFIGFAIRVALVPVHAWLPRVLSEAPPAVAMVLSSA
                     VPAAAIYGFIRINYSLFTEATIWFKDALAVISVVTILYSAFCALGQKDLRRLLAYSTI
                     GQFGFVFFGLTVISQNALHGAMLALICSVIINALMVYLVSV"
     gene            complement(1137..>2986)
                     /locus_tag="PLH57_06210"
     CDS             complement(1137..>2986)
                     /locus_tag="PLH57_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02242.1"
                     /translation="MLSTPYFLSLAAAFAGILIQLFAPKGKWIPGLPAVIMSALGFSG
                     VVWAVVLMSSGEVWNWEFSWFSPGPDQNLFINLNLGVGEKFWFLASAAAVFLLNIHFL
                     ILRIRAIRDSNERTIESGPEMAMPFFSMGFLLTSAAGNVLTFCGGWSFFIIGSLLTVY
                     VGRSNLDIKSTRTYRFFVTSVVGESILCVAALALSGFVPSGKLAEITPEVASRMPLWG
                     SIALLVGIIFRQSLLPVTKTVAHQASARGPGVLTAIFYQMLPLGILFHKFGVLFTHSQ
                     IQTVAAIVGLASALIVVLSKIASRDDSSFVCYLQTYFSSIALAMIAVGSASSSEMVIA
                     AAGFTLLAWMVLHWTVISTESALLASYPLWAQYSLGLVTVGVPFFGTGWSKFEWYRSL
                     LALSADPLLGQILLAFAVFIGLLYSFSIWGRVRLASLSNSENITTYQIGVVLFFLPVA
                     LLPIDLLQFSPEDILPLSLVIGEVFVGFVVAIFWIYRDAKIGTAFVERWNLKVSRPIA
                     GEVGSEYSYLEILFEKVLFLGSRILIVCETAGETGVFRTVGKKVFNVVTHVFHRLEHG
                     VLERLFVQNILRSLNGVVTAVKFVHNGAVQFYFILGVLLTAAIIFLTSK"
BASE COUNT         1014 a          733 c          595 g          644 t
ORIGIN      
        1 tcacactaac aaggtaaacc atcagggcat taatgattac tgaacaaatc aatgcgagca
       61 ttgcaccgtg aagtgcgttc tgagatatga cggttaatcc aaaaaaaaca aaaccaaatt
      121 gcccgatcgt cgaataggcg agcagtcttc gtaaatcttt ttggcccaac gcacaaaagg
      181 ccgagtagag aattgtgacc actgaaatca ccgctaacgc gtctttgaac caaattgttg
      241 cttcggtaaa aagactataa tttatccgaa taaagccata aatcgccgcg gccggaaccg
      301 cagagcttaa caccattgct accgcgggag gagcttcact caatactctg ggcaaccatg
      361 cgtgcaccgg aactaacgcc acacgaattg caaaaccgat aaacattaac aagtaagcac
      421 tcttttcgag atcccattcg cgatccagaa atagaatctt tgttccgttt aatttttgat
      481 cattcagcgc tccgaggtcg aacgtatgcg gttcggctgt gtaatataga agcaacatac
      541 ccataagaat gaacactgcc ccgattagct ggtatacaac atattcggta gccgctcgct
      601 cgcgatcttt cgcaccccaa acaccgatta gaaaatagag gggcacggat gccattagaa
      661 aaaacactag aaacaaaaac aaatcctgcg caagacacat tccgaaaacc gtactttgta
      721 aaagcagaaa cagcgcaaaa taacctttag cccccgatat ctgatagccg gactccgaaa
      781 aagatgcggc accacctttt atatatcgaa attgtttcgc ggcaaaaatg ctcaaaaaga
      841 atatgagtgc taccaaacag actagaaaga catttagacc gtcgagacca acgttatatg
      901 aaatatttaa actagatacc caacgatgct ggctcacaaa ctgtaaggtg ttactcgaaa
      961 aatcaaactc tactaaacag accacactca acaagagtgc gccgagtgca aaaacgctgg
     1021 ctaccaatct atgaatcggt cgatccccac taggtattag gcagattata gcagctccaa
     1081 ctatcggaag gagggttagg gtgctcaaaa gattatcgaa taacatggga atcctttcac
     1141 ttactggtca agaagatgat agccgccgtc aataataccc cgagaataaa ataaaactgc
     1201 accgcgccat tgtgtacgaa cttcactgcg gttacgacgc cgtttaaact gcgtagaata
     1261 ttttgaacaa ataatctttc gagcactccg tgttccaaac gatgaaaaac atgggtaaca
     1321 acattaaaaa cttttttgcc aacggttcga aaaacaccag tttctccggc cgtctcacaa
     1381 actattaaaa ttcgactgcc taaaaataat actttttcga aaagtatctc caaataactg
     1441 tactcgctgc cgacttcacc agctatgggc ctgctcactt tcaagttcca tctttcaaca
     1501 aaggccgtac ctattttggc atcacgataa atccaaaata ttgcgaccac gaaaccaacg
     1561 aatacttctc caatcaccaa cgagagcgga agaatgtcct ctggggaaaa ttgcagtaaa
     1621 tcgatgggaa gtaacgctac cggcagaaaa aatagtacga ctccaatttg atacgtagtg
     1681 atgttttcag aattcgatag actcgccaga cgaactcggc cccaaatgga aaacgaatac
     1741 aaaagaccta taaagacagc aaaagccaaa aggatttgac ccaacaatgg gtccgctgaa
     1801 agagccagca aacttcgata ccactcaaac ttggaccatc cagttccaaa aaaaggaaca
     1861 cccacagtca ccagtcctag ggaatattgg gcccagaggg gataggatgc gagaagtgcg
     1921 ctttcggtag aaattacagt ccagtgcaaa accatccacg ctaacagcgt gaaccctgca
     1981 gccgctataa ccatttcgga ggaagacgcc gaacccaccg cgatcatcgc caaagcgata
     2041 gacgagaagt aggtctgtaa atagcaaaca aaactactgt catctctgct ggcaattttg
     2101 gacagaacaa ctatcagagc tgaggcaaga cctactatcg ctgcgacggt ctgaatctgt
     2161 gaatgagtga acagtacccc gaatttatga aacaatattc ccaggggaag catttgataa
     2221 aatatcgcag ttagtacccc gggcccgcga gccgacgcct gatgcgctac ggttttcgtc
     2281 actggcagta aagattgtct aaaaatgatg ccaaccaaaa gtgcgatact gccccaaagc
     2341 ggcattcgtg acgcgacttc cggagtaatc tccgccaatt ttccacttgg cacaaacccg
     2401 ctcaacgcca gagcagctac acacagaatt gattcgccaa ccaccgaagt cacaaaaaaa
     2461 cgatatgttc gggtggattt aatatcgagg tttgaccgcc ctacataaac cgttaaaagg
     2521 gaaccaataa taaaaaacga ccacccacca caaaatgtca gcacgtttcc agcagcagag
     2581 gtaagtaaaa agcccattga aaaaaatggc atcgccattt cggggcccga ttctatcgtc
     2641 ctctcgttag aatctcgaat ggcccttatt cttaaaatta agaaatgaat attcagtaaa
     2701 aatacagctg cagctgacgc taaaaaccaa aacttttctc ctacacctaa attaaggtta
     2761 atgaacaagt tttgatctgg gccgggggaa aaccagctaa actcccagtt ccagacttcg
     2821 ccggaggaca tcaatacgac agcccaaact acccctgaga aacctaatgc actcatgatt
     2881 acagctggta atccaggaat ccacttccct ttaggagcga agagctgaat aagaataccc
     2941 gcgaaggcag cggctaatga taaaaaatat ggggtcgata gcatta
//
LOCUS       DAOMXO010000258         2984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18669_length_2984_cov_5.100717, whole genome shotgun sequence.
ACCESSION   DAOMXO010000258 DAOMXO010000000
VERSION     DAOMXO010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18669_length_2984_cov_5.100717"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLH57_06215"
     CDS             <1..538
                     /locus_tag="PLH57_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRK02243.1"
                     /translation="NGLRRLRHENTSMRRREIGCLHEVLNPNEVIRGITSGFYENRTW
                     VIVVTNSRLVFLDKGMIYGLRQLDMSLHQISSVAHRTRLFFGEVEVATGSGTKTIGCI
                     AKGDVVKISNILSAEIHAVRNPKPAGSSAPATTSGGTNQSLAGELERLAELYSSGALD
                     EQEFRASKARLLGMEPRT"
     gene            539..1207
                     /gene="aat"
                     /locus_tag="PLH57_06220"
     CDS             539..1207
                     /gene="aat"
                     /locus_tag="PLH57_06220"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRK02244.1"
                     /translation="MQPIDFPDPATADEDGIVAIGGNLLPETLLAAYRKGIFPWPVPG
                     YPKKFPYLTWFSPDPRGILEFEDLHIPRSLKKAINRSDYTYSIDKNFKNVIGACKNVP
                     RQEKGTWITPDIVEAYIQFHQVGHAHSVEVWDGPDLVAGLYGVDAFGVFAGESMFTLV
                     DDGSKLALMYFIEHLKARGATWIDIQMVTPHMEAMGGKLIRRDAFLTRLAQTQALDLR
                     LFDR"
     gene            complement(1134..>2984)
                     /locus_tag="PLH57_06225"
     CDS             complement(1134..>2984)
                     /locus_tag="PLH57_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK02245.1"
                     /translation="KPASKNKKDSEVEAGFLLNLNHWATEGRIDPLIGRTTEIERVVQ
                     TLCRRRKNNPLLIGEAGVGKTAIAEGLALAIVQKEVPDLLLNAEVFALDLGALLAGAR
                     FRGDFESRLKKVITSLQKRTAAGKLPILFIDEIHTIVGAGSVSGSSLDAANLLKPVLA
                     RGEIRCMGSTTYTEYRNVFEKDSALARRFQKIEVSEPSSEDSIKILKGIRSKFEDHHG
                     LKFTDEAIRAAVELSTKHLPERFLPDKAIDLIDEAGARARIKKKSEVVASDIEEIVAA
                     IAKIPARSVATDQKARLKTLDHDLKISIFGQAPAIDALTTAIRLARSGLRAADKPTGS
                     FLFCGPTGVGKTELAKQLAHCLGVPFLRFDMSEYMEKHTVSRLIGAPPGYVGFEQAGM
                     LTEQVRKSPYSVVLLDEIEKAHPDIWNILLQVMDHGTLTDNYGRKADFRNVILIMTSN
                     VGARELERKPLGLTTGNGPLAQSHLVMQKEVEKAFSPEFRNRLDSIVYFNALDPVTIA
                     QIVGKQLAELESQLLARNVEIQVDEDVRAWLGEKGYDPVMGARPMARLIQEKIRKPLS
                     LEILFGALEKGGHVRVMLENDEPKLFVTGRTSEDPELESERVGLKTHPDG"
BASE COUNT          721 a          789 c          695 g          779 t
ORIGIN      
        1 aaatggtttg aggcggctaa ggcacgaaaa tacctcgatg aggcgcagag aaattggctg
       61 cctacacgag gtccttaacc ccaacgaagt tatccgtggc atcacgagcg gtttttacga
      121 aaatcgaacc tgggttatcg tcgtcacgaa ttctcgctta gtgtttctcg acaagggcat
      181 gatctatggc ttacgccaac tagatatgtc gctccaccag atcagttcag tcgcccatcg
      241 cacacggctt ttttttggcg aagttgaggt cgcaaccggt tcgggcacga aaacaatcgg
      301 ttgtatcgct aagggagatg tcgtgaaaat ttctaatatt ctttccgctg aaattcacgc
      361 ggtacgcaac cctaaacctg ccggctcttc agccccagca actactagcg gggggacaaa
      421 ccaatctttg gcgggggaac tagaacgcct ggcggaactt tacagttctg gtgccctcga
      481 cgaacaagaa tttcgggctt caaaagcgcg gcttttgggc atggaaccgc gtacgtaaat
      541 gcaacccatc gattttcccg acccagcaac agccgacgaa gacggtattg tcgcaattgg
      601 cggtaatcta ttgcctgaaa ccttgttggc cgcttaccgt aaaggcatat tcccttggcc
      661 agtacctggt taccctaaaa aatttccgta tctgacctgg tttagtccgg atcctcgcgg
      721 gatacttgaa tttgaagatc tacacattcc acgttctcta aaaaaagcta tcaaccgatc
      781 agactacacc tattcgatcg acaaaaattt caagaacgta atcggagcat gcaagaacgt
      841 tccccggcag gagaagggca cctggataac cccggacatc gtcgaagcct atattcagtt
      901 tcaccaagtg ggtcacgccc acagtgtgga ggtatgggat ggacccgatc tggtcgctgg
      961 gctctacggc gtcgatgcat tcggcgtatt cgccggtgaa agcatgttca ctttagtaga
     1021 cgatggctcc aagttagcac tcatgtattt tatcgaacat ttgaaagccc gcggcgctac
     1081 ttggatagac atccagatgg tgacacctca catggaagct atgggcggca agctcatccg
     1141 tcgggatgcg tttttaaccc gactcgctca gactcaagct ctggatcttc gcttgttcga
     1201 ccggtaacaa ataatttagg ctcgtcgttt tcgagcatca ctcgtacgtg accacccttt
     1261 tcgagcgcgc caaagagaat ctctagcgac agtggctttc gaattttttc ttgaatcaac
     1321 cgcgccattg gtcgcgcacc catgactggg tcgtaccctt tttcgcccaa ccaggccctc
     1381 acgtcttcgt cgacctgaat ttctacgttg cgagccaaaa gttgcgattc aagttccgcc
     1441 agttgtttgc ctacgatttg ggcaatcgtg accggatcta aagcattgaa atatactatc
     1501 gaatccaacc ggttgcgaaa ctcggggcta aacgctttct cgacttcttt ttgcataacc
     1561 aaatgacttt gggcgagtgg tccgttgccc gtggtgaggc cgagcggctt gcgttcaagt
     1621 tcgcgcgcgc caacattcga agtcattatt aaaatcacgt tacgaaaatc agctttacgg
     1681 ccgtagttgt cggtaagtgt gccgtggtcc attacttgaa gaagaatatt ccaaatatca
     1741 ggatgagctt tttcaatctc gtcgagcaac actactgagt acgggctctt acgaacctgt
     1801 tccgtaagca tgcccgcttg ttcaaatcca acataaccgg gaggggcccc gatcaggcgc
     1861 gacacagtgt gtttctccat gtactcactc atatcgaaac gtaaaaacgg cacgcctaaa
     1921 caatgcgcca gttgtttagc aagttcggtt ttgcctactc cggttggtcc acaaaataaa
     1981 aaactacccg tcggcttatc ggcggcgcgc aaccctgagc gagccaatcg aatcgcagtg
     2041 gtcaatgcat cgatcgccgg cgcctgacca aaaatggaaa tttttaaatc gtgatcgagg
     2101 gtttttagcc gggccttttg atcggtcgct acagagcgcg ccggtatttt tgcgatggcg
     2161 gcgacaattt cttcgatgtc actggccacg acctctgatt ttttcttgat tcgagctcgc
     2221 gctcccgctt cgtcaatcag gtcaatggcc ttgtccggca agaatcgctc gggcaaatgt
     2281 ttagtggaca actctactgc cgcgcgaatg gcctcatccg tgaatttcag tccgtggtga
     2341 tcttcgaatt tcgaccgtat tccctttaaa atctttatcg agtcttcact cgatggttct
     2401 gaaacttcga ttttttgaaa ccttctcgca agagcagagt ccttttcaaa tacattccga
     2461 tactcggtgt aggttgtaga ccccatgcac cgaatttccc cccgcgcgag caccggtttt
     2521 aacaagttcg cagcgtctaa cgaactgccg gacactgacc ccgcgccaac tatcgtgtga
     2581 atttcgtcaa tgaacagaat aggtaacttg cccgccgcgg ttcgtttttg taacgacgtg
     2641 ataacttttt ttaaacgact ttcgaagtca cctcgaaagc gcgctccggc aagaagtgct
     2701 cctaaatcca aagcaaaaac ttcggcattc aaaagcaagt cgggcacttc tttttgaacg
     2761 atcgcgagag ccaggccctc ggcaatcgca gtttttccca ctcctgcttc accaattaac
     2821 agtggattat tctttctacg ccgacagagt gtctgcacca ctcgctcaat ctccgtagtt
     2881 cttcctatca gagggtcaat tcgaccttcg gtcgcccagt gatttagatt caaaagaaat
     2941 cccgcttcca cttcggaatc ttttttgttt ttagatgccg gttt
//
LOCUS       DAOMXO010000259         2983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18691_length_2983_cov_4.387637, whole genome shotgun sequence.
ACCESSION   DAOMXO010000259 DAOMXO010000000
VERSION     DAOMXO010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18691_length_2983_cov_4.387637"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLH57_06230"
     CDS             <1..201
                     /locus_tag="PLH57_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02246.1"
                     /translation="LVIRGVTFGCLELLNRLGEDFYTDADKEVLATICEFAAKVIEQR
                     LVLAAVTKELIDLRKLDRGEAA"
     gene            198..1355
                     /locus_tag="PLH57_06235"
     CDS             198..1355
                     /locus_tag="PLH57_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRK02247.1"
                     /translation="MSNPLRMLNNNSTTANGNGSAHRPTMKYLLHALLKHGASDLHLK
                     AGRPPMFRINGKLVPAKLPELAADTVKEILYNILNAKLIHQLEEHMQVDCSFNMESMG
                     RFRANIYIQRGTVAGVIRMIPLTVPSIDSLGLPSVLKDLCVKKRGLILLTGSAGAGKS
                     TTMAAMVNHINEHRHSHIITIEDPIEFVYRDIKSSICQREVGTDALNMHEALVGSLRQ
                     DPDIITIGEMREIDTIQAALTAAETGHLVLSTLHTNDARSTVDRILDVFPANSKNQVR
                     VQLSASLLAVVSQKLVMRADGEGRIAVCEVMIKSPTIENLILNNELDKIDDAIATSTT
                     YYKMQTFNQTLERLVLAGVITNEEAIYASNQPEDLKMRLSGFKREEGYNHG"
     gene            complement(1356..2204)
                     /locus_tag="PLH57_06240"
     CDS             complement(1356..2204)
                     /locus_tag="PLH57_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRK02248.1"
                     /translation="MDKLGFGTAGFPSKAPKKDPIAALHIVKDLGLDCLELEFAHGIR
                     MRDESALEINRTARKLGITLTAHAPYYINLNAKEQDKIDASINRLLQTARLAGMCGAY
                     SMTFHAAFYMKDEPGTVFGIVKRYLKHITDRLVDEQNPIHLRPELTGRKSQFGTLEEL
                     CELSQEVHQVYPCLDFSHDQAKTDCNDFEHFSQVFERYRKALGKSALGQMHIHVAGGQ
                     YGSKGEIRHLSLKDSGFNYKDFLRACKEFKIRGSVICESPELENDALLLKKTWEQLSE
                     KKSAKN"
     gene            2437..>2983
                     /locus_tag="PLH57_06245"
     CDS             2437..>2983
                     /locus_tag="PLH57_06245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRK02249.1"
                     /translation="MTNHLKVLIFFGVIATITTALGVLLFGNSGLPIGLAVGVAIVVS
                     LVGLSDWLVLKIHRAQILRSAESMGVHDIIERLAERARISVPQIYLSPSHSLSAFSVG
                     RGPHHASIVVTEGLLAILSYDELEGVLAHEVAHIKNRDTFVGTIAAVVAIGCLACAKL
                     SSWGAHHVQHLPLHDHVAGDGR"
BASE COUNT          798 a          664 c          712 g          809 t
ORIGIN      
        1 cttgtcataa gaggcgtaac gtttggttgc cttgagttat tgaatcgctt gggagaggat
       61 ttctatactg acgccgacaa agaggttctc gctactattt gcgaatttgc cgccaaagta
      121 attgaacaac ggttggttct cgctgccgtc accaaagaac ttatcgatct aagaaagtta
      181 gaccgtggag aggccgcatg agcaaccctc ttcgcatgtt gaataacaat agtacgacag
      241 ccaatggtaa tgggtcagcc caccgtccga ccatgaagta tttgttgcat gctttgttga
      301 aacatggagc ctcagatttg cacctaaagg ctggtcggcc accaatgttt cgaatcaacg
      361 gtaagttggt gcccgcaaag cttcctgagc tagctgcgga tactgtaaaa gaaattttgt
      421 ataacatttt aaatgcgaaa ctcattcatc aactagaaga acacatgcaa gtggattgta
      481 gtttcaacat ggaatccatg ggtcgatttc gggcgaatat ttacattcaa cgcggaacgg
      541 tagcgggtgt aatacgcatg ataccgctca cggttccaag catcgattcg ctcggattgc
      601 caagtgtcct gaaagatctt tgtgtaaaga aacggggcct catactcctg accggtagtg
      661 cgggagccgg taagtccaca accatggccg caatggtaaa tcacattaac gagcatcggc
      721 attcccatat aattacgatc gaggatccca ttgagtttgt ttaccgtgac attaaaagca
      781 gcatttgcca gcgcgaagtc ggcactgacg cactaaatat gcacgaggct ttggttggtt
      841 ctttgcggca ggaccccgac attatcacga taggtgaaat gcgagagatc gacaccatac
      901 aagcggcatt aaccgccgca gaaacagggc atttagtact ttctactcta cacacgaacg
      961 atgcgcgttc tactgtagac cgcatactag acgtgtttcc ggcaaactct aaaaaccaag
     1021 tcagagtcca attgagcgcc agcttgctag cggtagttag tcaaaaactt gtgatgaggg
     1081 cagatggcga aggtcgaata gccgtctgcg aagtaatgat taagagtcct actatcgaaa
     1141 atttaatact gaataacgaa ctcgacaaaa ttgacgacgc tatcgcaact tctacgacat
     1201 actacaaaat gcagacattc aaccagacgc tggaacgttt agtgttggca ggcgtaataa
     1261 caaacgaaga agcaatttac gccagtaatc aaccagagga cttaaaaatg agactctctg
     1321 gctttaaacg cgaagaaggc tataaccacg gatagctagt tcttggcaga tttcttttcg
     1381 ctcaattgtt cccacgtttt cttaagtaaa agagcgtcat tttcaagctc tgggctttcg
     1441 caaatcacag agcctcggat tttgaattcc ttgcaagcac gtagaaagtc tttgtaatta
     1501 aaaccactgt ctttgagaga caggtggcga atttctccct tagagccata ttgccctccc
     1561 gcaacgtgaa tgtgcatttg ccctaatgcc gatttaccga gggcttttct gtagcgctca
     1621 aaaacctgag aaaaatgttc aaagtcattg caatcagttt tggcttggtc gtgcgaaaaa
     1681 tctagacaag ggtaaacttg gtgtacctct tggcttaatt cgcatagctc ctcaagcgtt
     1741 ccaaactgac ttttccttcc agtaagctct ggtctcaagt gaatgggatt ttgttcatcc
     1801 acaagacgat cggtaatatg tttgaggtat ctctttacga ttccgaaaac cgtgcctggc
     1861 tcatctttca tgtaaaacgc agcgtgaaag gtcatgctgt aggcgccgca cataccagcg
     1921 agtcgcgcag tttgtagaag acgattaatt gacgcgtcaa tcttgtcctg ctctttagcg
     1981 ttcaagttaa tgtaataggg cgcgtgagca gtaagagtaa ttcctaattt tctagcggtg
     2041 cgattaattt ctaacgccga ttcatcgcgc atgcgtatgc cgtgagcgaa ttctagctct
     2101 aaacaatcta gccctaggtc tttaacaatg tgtaaagcag cgatggggtc ttttttggga
     2161 gccttgctcg gaaagcccgc ggtgccgaaa cctaatttgt ccatagaaga tgaatctcct
     2221 gtcacatcaa gattgagtgt gaccatttag catgtagacg aaagcgcatc aagtgtccgt
     2281 gtgggacttt aaaattcgtt aactgtcgaa acttgctggg gaattctgta acacgcacat
     2341 gaccatttgc gttagaaatt tgcaaaattt tgcggaaaat ctgttggaac gaattgtctt
     2401 gattatctaa aggcgaagtc gcttaaatgg agtggcatga ctaaccattt gaaagtcctc
     2461 atattttttg gagttattgc caccattacc acggctttag gagtgttgtt atttgggaat
     2521 agtgggcttc ctattggcct tgcagtcgga gtggcaatcg tagtttccct ggtcggtcta
     2581 tcagactggc tcgtgctcaa gattcatcgc gcgcaaattt tacgttcagc tgaatcgatg
     2641 ggcgtacacg acattattga gcggttggca gaacgagcta ggatttcagt gcctcaaatc
     2701 tatctttcgc caagtcacag cctaagcgct ttttcggtgg gccgcggccc tcatcatgct
     2761 tcgattgttg ttacggaagg gctcttggcg attctgagtt acgacgaact cgagggcgtt
     2821 ctcgcccacg aagtcgccca cattaaaaat cgtgacactt ttgttggtac cattgcagca
     2881 gttgtcgcaa ttggttgtct tgcatgtgcg aagttaagca gttggggggc ccatcatgtc
     2941 cagcatctgc cgctgcacga tcatgtggcc ggtgacggtc gca
//
LOCUS       DAOMXO010000260         2983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18693_length_2983_cov_3.469604, whole genome shotgun sequence.
ACCESSION   DAOMXO010000260 DAOMXO010000000
VERSION     DAOMXO010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18693_length_2983_cov_3.469604"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLH57_06250"
     CDS             <1..545
                     /locus_tag="PLH57_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK02250.1"
                     /translation="EFPKIKKALTDLGANVVFSVATHAHLDHIGAFSELKSFSPKTQI
                     ALHRDDEFLYKILPDQGRMFGFSYDMPPLIDRYLQDEEEIQFGQQKFSVIHTPGHSPG
                     GICLRFEAGSLAPEPVVFSGDTLFRSSIGRTDLLGADHSQLIKSIKQRLLTFDDQTRI
                     CPGHGPNSSVGLERRENPFL"
     gene            615..1409
                     /locus_tag="PLH57_06255"
     CDS             615..1409
                     /locus_tag="PLH57_06255"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRK02251.1"
                     /translation="MRGVVYSAFILIGSILTLGVLVSGCGGHGRALIVKSEPGDAEVC
                     IKGKAGSEYFHNQKSCVGTTPFEAHKVKALNPDGERHDVKFSSVEGDRESFYVVVSRP
                     GYVSEVLEVPGKAGEWDFNLFLKSEQAAAAAQVAAVHSRAPVIPISVSPTEPHPANAQ
                     PQVGLGTVRVTSDPVGALVYINGGLRGNTPFTHEGRGGESIQIKLELDGYRPVEKNFT
                     LESQKNFVINFRLQSTADGASAAIVEPVVTPVASPVKNPEPNKNQQ"
     gene            complement(1400..2623)
                     /locus_tag="PLH57_06260"
     CDS             complement(1400..2623)
                     /locus_tag="PLH57_06260"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRK02252.1"
                     /translation="MLNDVSKSAQPKPALEGGAKTRSEFLVFGKPKFGPEEKAEILSV
                     LESGWVGTGPRAAAFEKQFAEYLWPTKPLHAIALNSCTAALHLAAVCLNLKPGDEVIV
                     PALTFCATANAVIHAGGTPVFADVDPVRLCVSAATIEKALTPKTKAIVVVHFAGRSAP
                     MDEIMALADQRGIRVIEDAAHAIETRWNDRHVGTYGWASCFSFYATKNVSTGEGGMLL
                     TRDEELASLVRKLSLHGMSKDAHKRFTTPGFHHQSYDYVGFKYNMPDLAAALGIHSLK
                     KVESNYALRKNIWEIYMNELRDLPLTLPPSAEFRENAHDRHALHLFQIRVKAALRDKL
                     LSAFSAENIGVGVHYPALTTMPLYEKYLKPGQTFKEAEIFSAETISLPIGTAMTEADA
                     HDVVRAAKKILSFYC"
     gene            2651..>2983
                     /locus_tag="PLH57_06265"
     CDS             2651..>2983
                     /locus_tag="PLH57_06265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK02253.1"
                     /translation="MSNLPKLSACVITKNEERVLARCLSSLKFADEIIVVDAHSSDQT
                     VEIAKAHGAKIISRPWSGFAAQRNVGLEQCSGDWVFFLDADEEASSLLGERLTKVARD
                     QHNRHPNCY"
BASE COUNT          751 a          713 c          765 g          754 t
ORIGIN      
        1 acgagtttcc gaagatcaaa aaagctctca ctgatctggg agcgaacgta gtgttttcgg
       61 ttgccactca cgctcatctg gaccacatag gcgcattttc agaactaaaa tctttttctc
      121 caaaaactca gatcgcactt catcgagacg acgagttttt atacaaaatt ctccccgatc
      181 aaggccgtat gtttggattt tcctatgaca tgccaccttt gattgatcgc taccttcagg
      241 acgaagaaga gattcaattc ggccagcaaa agttttcggt tatacacacc ccggggcatt
      301 caccgggcgg aatttgcctt cgtttcgagg cgggctcgct tgcgcctgag ccggtagttt
      361 tttcgggaga cactctgttt cgaagctcta tcggcaggac cgaccttttg ggcgctgatc
      421 attcccaatt gataaagagc attaaacaaa ggcttctaac tttcgatgat caaacccgaa
      481 tttgtccagg ccatggtccg aactcatcgg ttggcctaga gcgtcgcgaa aacccttttc
      541 tgtgatcgca aaaacttcct agtgatggat ctttgatttc ggtctaaaat cgacttacag
      601 ctctgggggg ttctatgcga ggcgttgttt attcggcatt tattcttatc ggttccatct
      661 taacgttagg tgttttggtt tcaggttgcg gtggccacgg tcgggcgctg atcgtaaagt
      721 ctgaaccagg cgatgccgaa gtttgcatta aaggaaaagc gggcagcgaa tattttcata
      781 atcaaaaatc ctgcgtaggc acgacgccat tcgaggcgca taaagtaaaa gcgctaaatc
      841 ccgacggcga acgccacgat gtcaaatttt ccagcgtcga aggggacagg gaatcttttt
      901 atgtagttgt cagtcgtccc gggtacgtca gcgaggtgtt ggaagttccg ggtaaagccg
      961 gcgagtggga ttttaattta tttctaaagt ccgaacaagc cgcggcagcg gcgcaggttg
     1021 ctgcagttca ctcgagagca cctgttattc caatttctgt atctccaacg gaaccgcacc
     1081 cggcaaacgc ccaaccgcag gtgggacttg gaacggtacg agttacgtca gacccggtag
     1141 gagcattggt ttacatcaac ggtggtttgc gcggaaatac gccttttacc cacgagggca
     1201 gagggggaga gtcaattcaa atcaagctgg agctcgacgg gtatcgccca gtggaaaaga
     1261 attttacgtt agaatctcaa aagaattttg taattaattt cagacttcag tctaccgctg
     1321 atggggcgtc ggctgctatt gtcgagccgg tagttacccc ggttgcgagt cccgtaaaaa
     1381 atcctgagcc aaataaaaat cagcagtaga aggaaaggat ttttttcgct gcccgcacga
     1441 catcgtgcgc atctgcttcg gtcattgcag tgccgattgg caacgaaatg gtttccgcag
     1501 aaaatatttc agcttctttg aaggtttgac caggtttcaa atatttttca taaagcggca
     1561 tagtggttaa ggcagggtag tggaccccca ctccaatatt ctcggctgaa aatgcagaga
     1621 gtagtttatc gcgaagtgcg gctttaactc gaatctggaa taaatgaagg gcgtgccgat
     1681 catgggcgtt ttcacgaaac tctgcagacg gaggcagcgt gagaggtaag tctctcagct
     1741 cattcatgta aatttcccaa atattcttac gaagcgcgta gttggattcg acttttttca
     1801 gtgagtgaat tccaagtgct gccgcaagat ctggcatgtt gtacttaaat ccaacataat
     1861 cataggactg atggtgaaac ccgggagtgg taaatcgttt gtgcgcgtct ttagacatgc
     1921 catgaagtga aagtttgcgc acaaggctag ctaattcttc gtccctagtg agaagcattc
     1981 cgccctctcc ggtagacaca ttcttcgtag cgtaaaagct aaaacaagag gcccagccat
     2041 aagtacccac gtgacggtcg ttccagcgcg tttcaatagc gtgagcggca tcttcgatca
     2101 ctcgaatgcc gcgttggtca gccaaggcca tgatttcgtc cattggggca gatctgcccg
     2161 caaaatgcac cacaacaata gctttggttt tgggcgttag tgctttttca atagtggccg
     2221 ccgatacaca aagacgaaca ggatctacgt cggcaaacac cggggtgccc ccagcatgaa
     2281 tcacggcgtt tgcggtggcg caaaacgtaa gtgcaggaac aattacttcg tccccgggct
     2341 tcaaatttag gcaaaccgct gccaaatgaa gggcggcggt acaagagttg agagcaatgg
     2401 cgtgtaaagg ttttgtcggc cagaggtact cggcgaactg cttttcgaag gcggcagccc
     2461 gtggacctgt gccaacccaa cccgattcaa gcacgcttag aatctcagct ttttcttcgg
     2521 ggccaaactt tggtttgccg aataccaaaa actcggaacg agttttggcg ccaccctcta
     2581 gggcgggttt aggctgggcg gatttcgaaa cgtcgttgag cattgccgta ccttagcata
     2641 cgatgtcgac atgtccaatc tgcccaaatt atcggcctgc gtaattacta aaaacgagga
     2701 acgggtgctg gcgcgctgtc tttcgagtct taaattcgcc gacgaaataa tcgtcgttga
     2761 cgcacattcc agcgaccaga ctgtagaaat agctaaagcc cacggggcaa aaattatttc
     2821 gcgcccttgg tctggttttg ccgcccaacg caacgtcggg ctggaacagt gcagcggaga
     2881 ttgggtcttt ttcctagacg ctgacgaaga agcttcttcg ttgctgggtg aacgcttaac
     2941 caaagtcgct cgagatcaac acaaccggca ccccaactgt tat
//
LOCUS       DAOMXO010000261         2982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18707_length_2982_cov_4.134267, whole genome shotgun sequence.
ACCESSION   DAOMXO010000261 DAOMXO010000000
VERSION     DAOMXO010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18707_length_2982_cov_4.134267"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..636)
                     /locus_tag="PLH57_06270"
     CDS             complement(31..636)
                     /locus_tag="PLH57_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRK02254.1"
                     /translation="MLSISSGLLRGLSLTSPPEKLAHEGVRPTGQKTRQAVFNVLRHF
                     QFCGEPILAGAVVADLFAGAGTLGLEAVSNGAKSCVFLEAHRAVLPVLQRNVAIVAEK
                     MLKQGHEVQLSVLPGDALSNYSRLPSIRILFADAPYKAAVALQLLETEAKSSKIDQNG
                     LLILEVSIAEKVPGQVGALVRFDEKIYGDTAVHFFVKKGFA"
     gene            complement(658..1170)
                     /locus_tag="PLH57_06275"
     CDS             complement(658..1170)
                     /locus_tag="PLH57_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02255.1"
                     /translation="MVQGDKEVAQPQDDTGEIDRIMKEIEELEKNMGAAPAAAPAAGP
                     VVAPEAAVAPAPVKDAATQEPVAPVSAETNEDDNMHHNVVPLHADSGDSESLVESDAV
                     LTNNEPLMKSDSQYEGDGALALKVGGCAEVQLEFTRAGVAVTLHCSDDGLSITTDQGA
                     EFRIPFRKAA"
     gene            1280..>2982
                     /locus_tag="PLH57_06280"
     CDS             1280..>2982
                     /locus_tag="PLH57_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02256.1"
                     /translation="MGIEQTSSERSQLSQLLNTKTLLGRVVTDDPELGNSNPMVFALS
                     KGGISTSATQSAPTSALRLDPQLSVSLEQHPLNAAFEILTKDVRASRDRLLALNPGRR
                     TAIERRLEFIARKVARARGEKLNRSLSLTDILRESYRETDFGRTLGLAAWEGFVAEVA
                     LFQILGIFFLKHLGVLGWRSFENADLGRLNFTIHSFLQNCAQGFSYDKHCWNFVRSNL
                     YSWYVPSTAAQQQLAAALEQFNNKSENFEWSDHSARKWMSTTPLRRQFRHLDFDGERK
                     VAAFCEEVLEKLGVTYLSSFHGETVCKKTFLPCLELGDFSLNLVEKLLTKLGGECTDI
                     CTDSSQVSRAIWACESELFEVSFTEMMGLLALLRHLKECSPTRTRLPQILHIAPLMGL
                     ELHNLEQLPLGGPDVSRLHQGTAGQIQQIEGFDQALVLDHIDRSKSTRWLKALSEQLP
                     HWRGLLSSSSNLNWGELHLNLAISKLKENGTCVYLSHRVLPDSHDGERLRKALLGMAT
                     LEYFVELTGEELAPYRYLYVFRRVHNKNERDLHRPRFGKFGTNRIDFTELEDSSATQH
                     EL"
BASE COUNT          725 a          791 c          699 g          767 t
ORIGIN      
        1 ctggataaag tgcgcgtcgt attaggcggt tcatgcgaaa ccttttttta caaaaaaatg
       61 aacggccgtg tcaccgtaaa tcttttcgtc aaaacgcacg agcgccccta cctgaccggg
      121 caccttttcg gcgatcgaga cttcgagaat tagaagacca ttttgatcta ttttcgaact
      181 tttagcctcg gtctcgagca attgcagggc gactgcggcc ttatacggcg catcggcgaa
      241 caaaattcga atacttggta gcctcgagta gttggaaagt gcgtctcccg ggagaacgct
      301 caattgcact tcgtgccctt gcttgagcat tttttcggcg acgatcgcga cgtttcgctg
      361 caataccggc aaaacagcgc gatgagcttc caaaaataca caacttttag cgccattgga
      421 cacggcctca agcccaaggg ttccagctcc cgcaaacaaa tcggccacta ctgccccggc
      481 caaaataggt tcaccgcaaa actgaaagtg gcgcaacaca ttaaaaaccg cttggcgggt
      541 tttctgtcca gtcgggcgca cgccctcatg agcaagcttt tcgggtggag aagttaagga
      601 aaggccccgg aggagccccg atgagatact gagcatctag cgatgctttt accggactta
      661 ggctgctttt cgaaagggaa ttcggaattc ggcgccttgg tcagtagtga tcgaaagtcc
      721 gtcgtcagaa cagtgaagag tcactgccac gccggctcga gtaaactcga gctgaacttc
      781 cgcacaccca ccgactttca acgccagagc accgtcgccc tcgtattgag aatcagattt
      841 cataagtggt tcgttgttag ttaacacggc gtccgactca actaaactct cggaatcgcc
      901 cgaatctgca tgaagcggca caacattatg atgcatattg tcgtcttcgt ttgtttctgc
      961 cgaaactgga gcaactggct cttgcgtcgc agcatcctta actggagctg gggcaaccgc
     1021 ggcttcgggc gcgaccactg gacccgctgc aggcgctgct gcaggcgctg cacccatgtt
     1081 tttttcgagc tcttcgatct ctttcatgat gcgatcgatc tcacccgtat cgtcctgtgg
     1141 ctgtgcgact tctttgtcac cctgtaccat agattctcct ctccgagcat attgttaaga
     1201 ccgtgcccgg ttccgctatt aattttttcg gcaagtcttc aaatttctga agtctttcgg
     1261 tactaatttc agaacagaca tgggcatcga gcaaacttca tccgaacgat cacaactgtc
     1321 ccaactcctg aataccaaaa ctcttttggg ccgggtcgtc accgacgatc ccgagctcgg
     1381 caattccaac cctatggttt ttgcccttag caagggtgga atcagcactt cagcgacaca
     1441 atcggcacca acttcggcgc ttcgcctcga tccccagctg agcgtttcgt tggaacaaca
     1501 tccactaaat gcggcgtttg aaattcttac caaagacgta cgcgcatcac gagatcgact
     1561 gctggcattg aatccaggaa gaagaaccgc tattgagcgc cgtcttgaat tcattgctcg
     1621 caaagttgca cgagctcgag gggaaaaact caatcgttcg ctttccctca ccgatatttt
     1681 gcgtgaaagc tatcgtgaaa cagatttcgg ccgcacgctt ggcttggcag cctgggaagg
     1741 tttcgtagcc gaagtcgcgc ttttccaaat tttaggaatc ttttttctaa aacacctcgg
     1801 tgttcttggc tggcgctcat ttgaaaacgc cgatcttggg cgacttaatt ttaccattca
     1861 tagttttctg cagaactgtg ctcaaggttt ttcctacgac aagcactgct ggaattttgt
     1921 tcgaagcaat ctgtactctt ggtatgttcc aagcactgcg gcccagcaac aacttgctgc
     1981 cgccctcgag cagtttaata acaagtcaga aaattttgaa tggagcgatc acagtgctcg
     2041 caaatggatg tcgaccacgc cccttcgaag acagtttcgg cacctcgatt tcgacggtga
     2101 aagaaaagta gccgcctttt gcgaagaggt tctcgaaaaa ctaggggtca cctacttaag
     2161 ttctttccac ggtgaaactg tttgtaaaaa aacttttcta ccgtgccttg aactcggcga
     2221 cttttcactc aacctcgtgg aaaaactact tacgaaactc ggtggtgagt gcactgacat
     2281 ttgcaccgat tcttcgcaag tttcacgtgc gatttgggcc tgtgaaagtg aactgtttga
     2341 agtttcattc acagagatga tgggtttgct cgcattgctt cgccacttga aggagtgctc
     2401 acctacacgc acacgactgc cccagattct tcatattgca cctctcatgg ggcttgaact
     2461 gcataatctt gagcaacttc cgctcggtgg tcccgatgtt tcgcgtttgc accagggaac
     2521 cgcaggacaa attcagcaga tcgaaggttt tgatcaagcg ctcgtattag atcatattga
     2581 tcgttctaaa tcgactcggt ggctcaaagc actttcagag caactgccgc actggagagg
     2641 gcttctttcg agcagctcca atctaaattg gggagagctt catttaaacc tcgcaatcag
     2701 caagcttaaa gaaaacggca cctgcgtgta tctctcccac cgagtactgc ccgattccca
     2761 cgacggcgaa cgtttgcgaa aagcccttct tggcatggcc acgctggagt atttcgtaga
     2821 acttacgggt gaagaactcg ccccttatcg ttacctttat gtttttcgtc gcgtgcataa
     2881 taaaaacgaa agagatttac accgaccgcg gtttggcaaa ttcgggacta atcgtatcga
     2941 tttcacggag cttgaagatt ctagcgccac tcaacacgaa ct
//
LOCUS       DAOMXO010000262         2980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18723_length_2980_cov_4.085812, whole genome shotgun sequence.
ACCESSION   DAOMXO010000262 DAOMXO010000000
VERSION     DAOMXO010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18723_length_2980_cov_4.085812"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2271
                     /locus_tag="PLH57_06285"
     CDS             <1..2271
                     /locus_tag="PLH57_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRK02257.1"
                     /translation="VRRISRTEGVQSVGIGIRKQRGSNAVAVAHEVRKRMVEVQNQLP
                     DHMKMAVNFDSTKFIEEAVHELNFTLLLSAILTALVCWLFLGSWSSTINVVLAIPTSM
                     IGTFIVLYFSGFTLNTFTLLGLSLAIGIVVDDSIMVLENIVRHFEMGKNRVQAALDGA
                     KEIQFAALAATIAVIAIFLPVVFMKGAIGKFFFQFGVTMTVAVALSLVEALTLTPMRC
                     AQFLQKTERKTRLGRFVEQSFLESAAVYKRILTFALGHRWKVLTVSVLICGASVSIVK
                     LLNKEFIPPQDQGRFMVRVQTPVGSSLDFTNEKFKIAEAELSKDPGIERYYVAVGGFG
                     GGEVNSGMMFVTLKDLKLRKKNGDTQKVVMDRVRKVFSKIPETKAFVQDLSMRGFTAS
                     RGFPLEFTVRGPEWDEIAKNARVIMDRLSASGMVSDLDTDYKEGMPEVRVIPDRKKAS
                     MRGVSVAAIAELINATVGGVVAGKYSKGGHRYDIRVRLEAAERSSIENLKALFVRNNR
                     GELISMADVVRVEEKPTLQVISRRDRERAISVFGNIATGKSQSLVLDEVQKIAKEVLP
                     EGYRVVLTGGSESFKESFNSLVFALVLGLVVSYMVLASQFNSFVDPLTVLMALPFSIS
                     GAFVALWLAGQSLNIYSMIGLILLMGIVKKNSILLVDFTNQIRAKGKNVFEALIEACP
                     NRLRPILMTSIATIVGAVPPALAIGPGAESRIPMAISVIGGVLVSTILTLFVVPCVYS
                     LFATNRKIVNEDHIRY"
     gene            complement(2329..>2980)
                     /locus_tag="PLH57_06290"
     CDS             complement(2329..>2980)
                     /locus_tag="PLH57_06290"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRK02258.1"
                     /translation="ITHFRWYLFKTKETEVRHFIDTYLKPNETLFDVGANIGVFSVYA
                     AKRGLRVFSFEPEYSNLALLKKNILRNRVHANIQTYCVGISDFVGLSKLHLQSTLGGA
                     AAHTESTSAIQSTSEGFAVVFSEGIVTATLDYLCENLGVVPNGLKIDTDGNEPKILSG
                     APKLLKNPVLKSIIIEIPSDPDKKRFCEDILIQNGFHCDWSAQSSKNQIWVRIKNI"
BASE COUNT          771 a          609 c          737 g          863 t
ORIGIN      
        1 gttcggcgaa ttagccgaac cgaaggcgtt caatcggtgg ggattggaat tcgtaagcaa
       61 cggggatcca atgcggtcgc agtagcgcat gaggttcgca aacgcatggt cgaagttcag
      121 aatcaactgc cggatcatat gaaaatggca gtaaattttg acagcacaaa atttatcgaa
      181 gaagcagttc acgaactcaa cttcacgctt cttctttcgg caatactaac ggcacttgtt
      241 tgctggctgt ttttaggatc ttggtcatcc acgataaatg tcgttctcgc gattccgact
      301 tcaatgatcg gtacgtttat cgtgctctac ttttcagggt ttacgcttaa tacctttact
      361 cttctcggtc ttagtttagc gatcggcatc gtcgtagatg actcgattat ggttcttgag
      421 aacattgtcc gccatttcga aatgggaaaa aatcgcgttc aagcggcttt agatggagct
      481 aaggaaattc agtttgcggc tctagctgcg accattgcag tgatcgccat atttttgcca
      541 gtggtattta tgaagggagc catcggaaag ttcttttttc aatttggcgt cacaatgacg
      601 gtagcggtgg cactttcgtt agttgaagcg ttgacgctca ctcctatgcg ttgtgcgcaa
      661 tttttacaaa aaactgaaag aaagaccagg ttggggcgat tcgtcgaaca gtcgttcctg
      721 gaatcggcgg ctgtgtataa aagaattcta actttcgcac tggggcatcg ttggaaggtg
      781 ctcactgttt cagtcctgat atgtggggcc tctgttagca tcgtgaaatt actcaataag
      841 gagtttattc cgccacagga ccaaggacgt ttcatggtcc gagtacaaac tccagttggc
      901 tcttcgctgg attttaccaa tgaaaagttt aaaatcgcgg aggccgaact ttcgaaagat
      961 cccggaatag agaggtatta tgtcgcggtt ggtgggttcg gtggaggtga agttaactcc
     1021 ggaatgatgt tcgtcactct taaggactta aaattaagaa aaaaaaatgg agacactcaa
     1081 aaagtagtca tggatcgagt gcgtaaggta ttttcgaaaa ttccagaaac caaagcattt
     1141 gttcaggacc tctctatgcg aggatttact gcctcgcggg gatttccgtt agagtttacc
     1201 gtccggggcc ccgagtggga cgaaattgca aagaacgcca gagtcattat ggaccgactt
     1261 agtgcttcgg gtatggtgag cgatttagat acagattaca aagaaggaat gcccgaagtg
     1321 cgggtgattc ctgaccggaa aaaagcatcg atgcgtggtg taagtgtggc cgcgatcgca
     1381 gagcttatca atgcgaccgt aggcggggtc gtggctggaa agtactctaa aggcggacat
     1441 aggtatgata ttcgggtccg tctcgaggct gccgaacgta gcagcataga aaatttgaag
     1501 gcactgtttg ttcgtaataa tcgtggcgaa ctaatttcca tggccgatgt ggttcgagtc
     1561 gaagaaaaac ccactcttca ggttatttca cgcagagatc gcgagcgtgc gatctctgtt
     1621 tttggaaata ttgcgacggg taaatcgcag tctttagttt tggatgaagt ccaaaaaatc
     1681 gcaaaagaag tgttgccgga ggggtatagg gtggttctta ctggcggctc ggagagtttt
     1741 aaggagtctt tcaatagtct cgtattcgcg ctggtattag gtcttgtggt ttcttacatg
     1801 gtgttggcct cccaatttaa cagttttgta gaccctttga cggttttaat ggcgctgccg
     1861 tttagcattt ccggtgcctt cgtggcgctc tggctagcgg gccagtcact caatatttac
     1921 agtatgattg gcctgatttt attaatggga attgtaaaaa agaattccat cttgctggta
     1981 gattttacga atcaaattcg cgctaaagga aaaaatgttt tcgaagctct gatcgaggct
     2041 tgcccaaatc gtttgcggcc cattttaatg acttcgatcg ctacgattgt tggagcagta
     2101 ccaccggcac tcgcaattgg gccgggcgcg gaatctcgta ttcctatggc gatttcagta
     2161 attggcggag tattggtttc aacgatattg accttattcg tcgtgccttg cgtttacagt
     2221 ctttttgcga cgaaccgtaa aatagtaaat gaggatcaca ttagatatta aaaatatttt
     2281 tcaacatgtt tttttgcaca cgcgcggtcg tcgcccattg caactcggtt agatattctt
     2341 aatacggacc caaatctgat ttttcgaact ctgagctgac cagtcacagt gaaacccatt
     2401 ctgaattagt atgtcctcac aaaagcgctt tttatcggga tccgaaggaa tttcaatgat
     2461 gatgcttttt aataccggat tctttaaaag tttgggcgca cccgaaagaa tttttggctc
     2521 gttgccgtca gtatctattt tcaacccatt tggaactacg cccaaatttt cgcaaaggta
     2581 gtcgagcgtc gctgtaacaa taccttcact aaagacgaca gcgaaacctt cactcgtact
     2641 ttgaatggca ctggtacttt cggtgtgcgc cgccgcaccg cccaatgttg attgaagatg
     2701 taatttacta agaccgacga agtcactgat cccaacgcag tatgtttgaa tgtttgcgtg
     2761 aactcgattg cggagaatat ttttttttaa caatgcgaga tttgagtatt cgggctcgaa
     2821 actaaaaacc cgtaatcccc gctttgcggc atagaccgaa aaaactccaa tgttcgctcc
     2881 gacgtcaaaa agtgtttcgt tcggctttaa ataagtatca ataaagtgtc gtacttcggt
     2941 ctctttcgtt ttgaagagat accaacgaaa atgtgtgatc
//
LOCUS       DAOMXO010000263         2977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18740_length_2977_cov_5.579055, whole genome shotgun sequence.
ACCESSION   DAOMXO010000263 DAOMXO010000000
VERSION     DAOMXO010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18740_length_2977_cov_5.579055"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLH57_06295"
     CDS             <1..299
                     /locus_tag="PLH57_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02259.1"
                     /translation="YVVANLHNEVLKKKIPLRFLIPGKLDFYDFDMTPHEAEDGGTAN
                     FKIEINNWFLRIFAPHLLVKYDRLSKRIVWYQGLSNLRTDEGDNQNVTIDYFYP"
     gene            311..811
                     /locus_tag="PLH57_06300"
     CDS             311..811
                     /locus_tag="PLH57_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRK02260.1"
                     /translation="MKLMSITLISFFSMAAIMLANTGHANNLERAKTFFDQLNKDQLH
                     LVDQFYDSQVVFQDPIHTLNGASALKKYYEGLYRNVDEIRFVYGPSIEQGNTVSLTWR
                     MHLQTPALQSGKELTVDGVSVITFGGTENKAIHHRDYFDMGEFVYERVPVLSSIITYI
                     KKRMAN"
     gene            917..>1184
                     /locus_tag="PLH57_06305"
     CDS             917..>1184
                     /locus_tag="PLH57_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02261.1"
                     /translation="MSEWFSTKVLSIGDAFSSAGVSPQKIWLVEGLPGEYQSKYAVSD
                     ETAASNPKILNAVSTGAGVGLGTGVALGAGVAVGAGVALGAGVAV"
     assembly_gap    1185..1284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1285..1993)
                     /locus_tag="PLH57_06310"
     CDS             complement(<1285..1993)
                     /locus_tag="PLH57_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02262.1"
                     /translation="MKSIQTRNYKMTASVLAILVLIAGGNLAGCEGVGLQAGGGGEGK
                     SKHESSDRCVHESIDGSKRGRNSVAVDLVRERPSQRIVGLDFLRTDLDLTTVSVRANG
                     ESKVFFSFNEANEIQFLEDCGEDVTIEVVYCRAEKVEVCHRPPGNPENERTISVGRPA
                     LSAHLAHGDIEGPCGGVEPSPEPSASPEPSASPEPSASPEPSASPEPSATPAPTATPA
                     PSATPAPTATPAPSATPA"
     gene            2283..2504
                     /gene="infA"
                     /locus_tag="PLH57_06315"
     CDS             2283..2504
                     /gene="infA"
                     /locus_tag="PLH57_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRK02263.1"
                     /translation="MAKDDLIRIEGRVADTSGGGHYKVLLENGVTILAKLCGNMKRFN
                     IRVIVGDKVTVGISPYDSTHGLIVHRQKF"
     gene            2532..>2977
                     /locus_tag="PLH57_06320"
     CDS             2532..>2977
                     /locus_tag="PLH57_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRK02264.1"
                     /translation="MSKRFKRIHIEISNVCNLQCNFCPEVLRQKTVMQEPLFRKIVAQ
                     AAPLADELSLHLMGEPLGHPNLSAFIDICAGSNAPVNLTTNGTLLDAARTEVLLHRTV
                     RQVNFSLHSFEANFGERDVTGYLRKIFAFTHAAFERRPDLYINFRL"
BASE COUNT          730 a          708 c          664 g          775 t
ORIGIN      
        1 attatgtagt cgcaaacctt cacaacgagg ttttaaagaa aaaaattcct ttacgttttt
       61 tgattcccgg aaagctcgac ttctacgact tcgacatgac tccccatgag gccgaagacg
      121 gcggaaccgc aaacttcaaa attgaaatca ataactggtt tttgcgaata tttgctcccc
      181 atttattggt aaagtacgat cgtctttcta agcgaatcgt ttggtaccag ggtctttcaa
      241 acctaagaac tgatgaggga gataatcaga atgtcactat cgattacttt tatccctagg
      301 aggcttggta atgaaactaa tgtctattac cttaatttct tttttttcaa tggcggcaat
      361 catgttggct aacaccgggc acgctaataa ccttgagcgc gctaagactt tttttgacca
      421 actaaacaaa gatcaactgc acttggtcga tcaattttac gactcacaag tggtatttca
      481 agatcccatt catactctca atggagcgag cgccctaaaa aagtactacg aaggacttta
      541 ccgtaacgtg gacgagattc gattcgttta tggaccgagc attgaacagg gaaataccgt
      601 atctctcacc tggcgcatgc atttacagac tccagctcta cagagcggca aggaactcac
      661 ggtggacggg gtgtcggtta ttacattcgg aggcactgaa aacaaggcca tccaccaccg
      721 cgactacttc gacatgggag agttcgttta tgaacgcgtc cctgtactta gttccattat
      781 cacctacatt aaaaaacgca tggcaaacta gggcttggtg gtttttacaa ttacctgact
      841 tcgagacgcg ccaccaaact gatccactga acgagcctgg aagtagtaag tagtatttgg
      901 tcttagaccg ccgatagtga gcgagtggtt ttcaaccaaa gtactatcta tcggagacgc
      961 gttttccagt gctggagttt cgccccagaa aatctggcta gtcgaaggat tgcctggcga
     1021 gtaccagagc aagtacgcag tgtccgatga aacggccgca tcgaacccaa agatattaaa
     1081 tgcagtctca actggtgccg gcgtcggact aggaactggc gttgcacttg gagctggcgt
     1141 agcagttggt gccggagttg cacttggagc tggcgtagct gttgnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnngtgccg gagttgcgct tggagccggc gtagcagttg
     1321 gtgccggagt tgcgcttgga gccggcgtag cagttggtgc cggagttgcg cttggctctg
     1381 gcgacgcact tggttctggc gatgcacttg gctctggcga cgcacttggc tctggcgacg
     1441 cacttggctc tggtgacggt tctacaccac cacaaggacc ttcaatgtcg ccgtgcgcga
     1501 ggtgagcact caacgctggt cgtcctactg aaatcgttct ttcgttctca ggatttcctg
     1561 gcggacgatg gcaaacttct actttttcag cacggcaata aactacttcg atggttacgt
     1621 cttcgccgca gtcttctaaa aactgaattt cgttagcttc attaaaggag aaaaaaactt
     1681 tgctttcacc attggcccgt actgacaccg tcgtgagatc aagatcggta cgaagaaagt
     1741 ccaacccaac gatgcgctga cttgggcgct cccgcacgag atcaaccgca acagagtttc
     1801 gccctctttt agaaccgtct attgattcgt gaacacagcg atcagaggac tcgtgtttac
     1861 ttttaccttc ccctccacca cccgcttgga gaccgacgcc ctcgcatccg gcaagattgc
     1921 caccagcgat caatactaag atcgccagca ctgaagcagt cattttgtaa ttgcgggttt
     1981 ggatggactt cataaaaaaa cctcacttct actgacaact ccattttact aagaattgtt
     2041 cgctatgtta aatttattta ataatcatag ttaacaactt gctcttgttg tgtttttttt
     2101 gtcgcgaaat gagctaagtg agtgtggtca gcgaccgaat gtgtcgtttg aacccagcta
     2161 ctgcctatcg gcctcggcgg ctatatcgta ttcccgctta accactgact ttctttaaaa
     2221 gtatctattc gactccatta ctgttaccgt cggacctcaa tttaaacatg agggacccac
     2281 atttggctaa agacgatcta attcgcattg aaggtagagt ggccgatact tccggagggg
     2341 ggcattataa agtgctgctc gaaaacggcg taaccatttt ggccaaactt tgcggaaaca
     2401 tgaaacgctt caatattcgc gtgatcgtcg gcgacaaagt cacggtcggt attagtccct
     2461 acgattcgac tcatggtctt atcgtgcacc gtcagaaatt ttaacccttt cgaaacgtcg
     2521 gttgagtttg cgtgagtaag cgctttaaac gcattcacat cgaaatcagc aacgtatgta
     2581 atctccagtg caacttttgt cccgaggttc tacgccaaaa aacggtaatg caggaacctc
     2641 tttttcgcaa aatcgttgcg caagcggctc ctcttgccga cgaactcagc ttacatctca
     2701 tgggggaacc gcttggccac ccgaaccttt ctgcattcat agatatatgc gcgggttcta
     2761 atgccccagt gaatctaacc accaacggaa ccttactcga cgcggctcgc accgaggtgt
     2821 tgttacatcg taccgttcga caggtgaact tttcactcca cagcttcgag gcaaacttcg
     2881 gcgaacgtga tgtcactggt tatttaagaa aaatttttgc tttcactcac gcagcctttg
     2941 aacgccgacc agatctttat attaacttta ggctttg
//
LOCUS       DAOMXO010000264         2966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18852_length_2966_cov_4.519066, whole genome shotgun sequence.
ACCESSION   DAOMXO010000264 DAOMXO010000000
VERSION     DAOMXO010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18852_length_2966_cov_4.519066"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLH57_06325"
     CDS             <1..449
                     /locus_tag="PLH57_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02265.1"
                     /translation="NMASRSTLWGLLPELTENEIDDLMRKRDDPDVGKPWNDEKDFWS
                     SVGTTASGASALRSIQERFKKAGLKIITNESSFRIGIEATVGQSTRRIEAHVLVAGAP
                     KASKTDAPGSGRPQTQPMGAGINPPTVDSTTKKGSENLQLVYWRSI"
     gene            458..2227
                     /gene="pilM"
                     /locus_tag="PLH57_06330"
     CDS             458..2227
                     /gene="pilM"
                     /locus_tag="PLH57_06330"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRK02266.1"
                     /translation="MKILSIDLGSSSIKAVEFESSFGRLELADYKIVEVTEPAIEAAS
                     VPAAEVVASAGEGAAESGSGSESKDAANSAEPPKRQLLTAGQIAAIRQIFREKDYKYD
                     KLVVNVPRAWATSRVFHFPTKDRKVIQSSLVFELEDDIPFDLEKVTHDFAILNYDGHQ
                     STIFTAVIQKNDLRDTISELQMIGLDPDVITTEPWALSQLLRRTIPVEYRDRPFCVVN
                     LGAKQTGIHMFLGEGPVLSLSCPAAGDDLTRAIAQNYGLTFDQAEKAKIEGGFVLTKM
                     HLSEHAATITKEQEKFSGVITDALVPIVREIKQALISYKAQTKLVPKAIFLTGGTSQL
                     PNLQLYLEEILQIPVFQTKYMSQLVGDTLRLAESTESQIAVATGLAVSSIKAERFATI
                     NFRKDEFVKNRGWGSLSLKHYKRPLMFAAASLGFVYANIFAQWLILNRREAQQEQTLE
                     RSIKGVVGAVSPSVLRTYIVSPSVLRNDIKKEFAKYEQSNVVSPSKKSLSGFDLLNMV
                     SKKMPRDVVIDVTQFDFKEEKLLVKGYLAPESSVDRLQGALDDTGVLTGVAVVKQEMV
                     PKLGKRYFEITASVKGENNVETR"
     gene            2211..2819
                     /locus_tag="PLH57_06335"
     CDS             2211..2819
                     /locus_tag="PLH57_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02267.1"
                     /translation="MSRLDDLKASLKQIIDRLRESDAYSQARARYDELDSQKKLYVKL
                     GAAGAAMMLVVSTVLGGMVRVATLKSAIDEKEALIGYLNQSGDTLRRFKEKQSGAGAA
                     DMNAGLSSFLQGVIEEAGVNEEKVKVGSEVSADLDKAIQETKVEVTLSQINLRQVTKT
                     LYDITSKGSVRGTAITALDIQAKDDFSGWLDATFTVSVYKAK"
BASE COUNT          833 a          626 c          747 g          760 t
ORIGIN      
        1 taaacatggc ctcgcgctcc actctatggg ggcttttgcc tgaacttacg gaaaatgaaa
       61 tcgatgatct tatgcgtaaa cgcgacgacc ccgatgtcgg taaaccttgg aacgatgaaa
      121 aagatttttg gagttccgtt ggtaccacag cgtctggtgc ttcggcattg aggtctatac
      181 aagagcgctt taagaaggcg ggacttaaaa ttattaccaa tgagagtagt tttcgaattg
      241 gaatagaggc cactgtaggt cagtcgacgc gtagaatcga agcgcacgta cttgtggctg
      301 gtgctccgaa ggccagtaaa acggatgctc ctggttccgg taggccgcag acccaaccaa
      361 tgggtgcggg cataaatccg ccaaccgtcg attctactac gaaaaagggt tcggaaaacc
      421 tacaattggt ttattggcgg tctatttagg aggctatgtg aagattctca gcattgatct
      481 cggatctagt tcgattaaag ctgtcgaatt tgaatcaagc ttcgggcgtt tggagcttgc
      541 cgactacaaa atcgtggaag taactgaacc ggccatagag gcggcctccg ttcctgcggc
      601 cgaagtggtc gcatcagccg gcgaaggagc tgctgaatcg ggcagtggat ctgaatctaa
      661 agacgctgca aattccgccg aaccaccgaa acgccaacta cttacagcgg ggcagatagc
      721 cgctattcgc caaatttttc gcgaaaaaga ctataagtac gacaaacttg tcgtaaacgt
      781 accgagagcc tgggcgactt ctcgcgtttt tcattttccc actaaagatc gcaaagtaat
      841 tcaaagttct ctggtctttg aactcgaaga cgacattcct tttgatttag aaaaggtgac
      901 ccatgatttt gcgattctaa attacgacgg tcatcagtcg accattttta ctgcagtaat
      961 tcaaaaaaac gatttgcggg atacgattag tgaattgcaa atgataggac tggaccccga
     1021 cgttattact actgagcctt gggcgcttag tcagcttttg cgtcgcacaa ttcctgtcga
     1081 atatcgcgac cgcccctttt gcgtagtgaa tttaggcgct aaacaaaccg ggattcatat
     1141 gtttcttggc gaaggtccgg tactttccct tagttgtcct gctgcgggtg atgacctaac
     1201 tcgtgcgatt gctcaaaatt acggacttac atttgatcaa gcagagaagg ccaagatcga
     1261 aggcggattc gtacttacga aaatgcactt gagtgaacat gccgcaacca tcaccaagga
     1321 gcaggaaaaa ttctcaggag tgatcacgga tgcgttagtt ccgatcgtgc gagaaattaa
     1381 acaagcactc atttcctaca aagctcaaac caaattagta ccgaaggcga tttttcttac
     1441 cggcggaact tctcaattgc cgaacctaca attgtacctc gaagaaattt tgcaaatacc
     1501 ggtttttcaa acgaagtata tgtcgcaact ggtgggcgat acgcttcgat tggcggaaag
     1561 caccgaatcc caaatagccg tcgcgacggg gttggcggtt tcttcgatca aagcagagcg
     1621 tttcgcgact attaatttta gaaaagatga gtttgttaaa aatcgaggtt ggggttctct
     1681 ttctcttaaa cattacaaac gacccttgat gtttgcggca gccagtttgg gtttcgtgta
     1741 cgccaatatc tttgctcagt ggttaattct gaaccgtcga gaagctcagc aggaacaaac
     1801 attagaaagg agcattaagg gagtggtagg tgcagtcagt cccagcgtgc ttcgcactta
     1861 cattgtaagt ccatcagtat tgagaaacga tattaaaaaa gaattcgcca aatatgagca
     1921 gtcaaatgtg gtttcgccct ctaaaaagag tctctcaggt tttgacttgc taaatatggt
     1981 gagcaaaaaa atgccacgtg acgttgtaat agacgtaacg cagttcgatt tcaaagaaga
     2041 aaagctttta gtgaaaggct acctcgcgcc ggagtccagc gtggatcgtc tacaaggcgc
     2101 tctagacgac accggtgtgc ttaccggggt ggctgtcgtt aaacaagaaa tggttccaaa
     2161 attgggaaaa aggtatttcg aaatcaccgc ttccgtgaag ggagaaaaca atgtcgagac
     2221 tcgatgactt aaaagcatca cttaagcaga taatagatcg actgcgtgag tccgatgcct
     2281 actcgcaagc gcgagcgcgt tatgatgaac tcgattctca gaaaaagctt tatgttaaac
     2341 tcggcgctgc gggagcagct atgatgctcg tcgtaagtac ggtgttggga ggcatggtgc
     2401 gagtcgcgac gctaaagagt gcgatcgatg aaaaggaagc gttgatcgga tatctcaatc
     2461 agtctggcga tactttgcgg cgatttaaag aaaaacaatc tggggcaggt gccgcagata
     2521 tgaacgctgg tttaagttcg tttcttcagg gagtcattga agaggcgggg gtgaacgaag
     2581 aaaaagtaaa agtgggttca gaggtatcgg ccgacttaga taaggcgatt caagaaacta
     2641 aagttgaggt gactctttcc caaataaatc tgaggcaagt cacaaagacg ctctacgaca
     2701 ttacctcaaa gggaagcgtt cgcgggaccg ctattactgc gttggacatt caggctaaag
     2761 atgattttag tggctggctt gatgcgacct ttaccgtttc ggtttataag gcgaagtagt
     2821 ggagcaaatt tatgaatcaa aataacgaac cacagtacca agaaacgact ggttctttgg
     2881 atctttcaaa ggcggaaccg aaaagccgtt ttcgcaccct tggtttcgtt gcatttggta
     2941 gttttgcttt ttttgtattc ctcatt
//
LOCUS       DAOMXO010000265         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18910_length_2960_cov_4.097418, whole genome shotgun sequence.
ACCESSION   DAOMXO010000265 DAOMXO010000000
VERSION     DAOMXO010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18910_length_2960_cov_4.097418"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLH57_06340"
     CDS             complement(<1..314)
                     /locus_tag="PLH57_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02268.1"
                     /translation="MVNIFKFAMIVSFIFAAQPGFAAGPKEPKEPPCKEIVGRLSIED
                     LDGDDNVILNSAVKTAKFVAKNHKIPTRAELAAELKMTPAELKAFLAEIPGVTNISDF
                     VQ"
     gene            complement(318..1463)
                     /locus_tag="PLH57_06345"
     CDS             complement(318..1463)
                     /locus_tag="PLH57_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02269.1"
                     /translation="MGDYRSRIWGHTLKPLYFAFFLSLLLFQPSSSAARIYVYSDFDN
                     SLVEALRGAFKTHFRLYRITYRSNVLQSPIEGPETIDVSPRDLYRLVDREGRSLLARG
                     EGAPGPIGIKFKLEDGTIIEPGNYMIVAPQTFEKFLIDNRGKQNHLLNDLKAAFEADP
                     SGDKIRGKAWPLMQLMLAHPETAKYFGILSARAHARSEWIEMFEHLLAHKNEATRILH
                     LPDSRNIHGVSHEDYTVLTTSYEITKRKVEFVKQVTHNLQRRLNDEAHTIVLPEDDQR
                     TIDAMTKFAQAEANRNIGPIKFVIWNLGFRDEIEARPFPEIAVIEANQPPRAATLEDI
                     VGPLDRLSPEQQKLIPQINAQLSAAFVPDVSCSVITHSGKPIGERGE"
     gene            complement(1663..2829)
                     /locus_tag="PLH57_06350"
     CDS             complement(1663..2829)
                     /locus_tag="PLH57_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRK02270.1"
                     /translation="MRTTRTDNKRNTIVRKKSTAKPGARFSKSSSRTDSRTSARPDSR
                     SRMGDSRARTGDSRARTGDSRRRFSDSSTAERRPRRDGAGPRDGRPHRGGREERNARP
                     SRELKKKSFKGPWKKTAYSVSTPKTPYNPDQPAPVVNSALDPATIAAHAASGERLQKI
                     IANAGICSRREAERLILEGAVTVNGRTVTELGTKANPDKDKIKVNGSLILTDVERLYL
                     VFYKPKAVIAALKDPEGRPCIADYLRTVRERVLPVGQMDFNSEGLMLLTNDGDMLNNI
                     VKARGLPKLYMVKVKGHPDEKTLSSLKDGIYSAEGVVRFADLQIEQALRNKSWLKLEV
                     TEGARLDLRELLNRRGLLVDRIMRTAIGHLSIQGLEPGQLKFVKKADFAGLLKY"
BASE COUNT          671 a          694 c          811 g          784 t
ORIGIN      
        1 aattgcacga agtcggaaat attggttacc ccggggattt cagcgagaaa ggcttttagc
       61 tcggcgggag tcatttttaa ttcggctgca agttcagcgc gagtaggaat tttgtggttc
      121 tttgcgacga acttagcggt tttgacagca gagttcagaa ttacattgtc gtcaccgtca
      181 aggtcttcga tcgaaaggcg accgacgatt tccttacacg gcggttcttt aggttctttc
      241 ggacctgcgg cgaagccagg ttgagcggcg aaaataaatg aaacaatcat cgcgaattta
      301 aaaatgttca ccattgtcta ttctccccgt tcgccaatcg gttttccgga atgggttatt
      361 acgctacaag aaacatcagg aacaaaggct gcgctaagtt gggcgttgat ttgtgggatg
      421 agcttctgtt gctccggcga aagccgatcg aggggaccca cgatgtcttc gagcgttgcc
      481 gcacgtggcg gttgattggc ctcaatcact gcgatttcag gaaatgggcg agcttcgatc
      541 tcatcgcgaa agccgagatt ccatataacg aatttgatag ggccgatatt acgattggcc
      601 tcagcttgcg caaacttagt catggcgtcg atcgtgcgtt gatcgtcttc cgggagcact
      661 atggtgtgcg cttcgtcgtt taggcgacgc tgtagattgt gagtcacctg ttttacgaat
      721 tcaactttac gtttggtgat ttcgtagctg gtggtcaaaa ccgtgtagtc ttcgtggctg
      781 acgccgtgaa tgttacgtga gtcgggcaga tgcagtatac gggtggcctc gttcttgtgc
      841 gcaagtaggt gttcaaacat ttcgatccac tcagagcgcg cgtgggcccg agccgataga
      901 atgccaaagt acttcgcggt ttcagggtgg gcgagcatga gctgcatcaa aggccacgct
      961 ttgcccctaa ttttgtcgcc gctagggtca gcttcaaacg ctgctttgag atcgtttaag
     1021 agatggtttt gcttgccacg attgtcgata agaaattttt caaacgtctg gggcgccaca
     1081 atcatgtagt tacccggctc aataatggtg ccgtcttcga gtttgaattt aatgccgata
     1141 gggcccggcg caccttctcc gcgagccaac aggcttcgcc cttcgcggtc caccaaacgg
     1201 taaagatctc gtggggaaac gtcgattgtt tcgggacctt ctattggaga ttgtaaaacg
     1261 ttggaacgat aagtgattcg atagagtcgg aaatgagttt taaaggcgcc acgcagagct
     1321 tcaactaagc tgttatcgaa atcgctgtag acataaatgc gggccgccga agaagaaggt
     1381 tgaaagagca aaagcgaaag aaaaaacgca aaatataacg gtttaagagt gtgcccccag
     1441 attcgagatc tgtaatcccc catgcgccat cccccaatgg ttaaattgtg ttccccagag
     1501 cgtgactata gcgtcccgcc ataaaggtca atacccggcc accccaaata tgtggactgg
     1561 gaatagaaaa tcggaaaggg atggggccgt caagattcgg ggaaaaataa ctttatacgc
     1621 aacgcattat ttgagcgcta ggtttcggaa ttccaagttc acctagtact taagtaaacc
     1681 cgcgaaatct gctttcttta cgaacttgag ttggccaggt tcgaggcctt gaatcgacaa
     1741 atgtccgatc gcggttcgca tgattcgatc aaccaagagg ccacggcgat tgaggagttc
     1801 gcgcaaatca aggcgcgccc cttcagttac ttcaagttta agccagcttt tgttgcgcag
     1861 cgcttgttca atttgtaggt ccgcaaaccg aacaacgccc tcagccgaat aaataccgtc
     1921 tttgagtgag gaaagagttt tttcatcagg atgcccttta accttaacca tgtagagttt
     1981 tggcaggccg cgggctttga cgatgttgtt gagcatgtcg ccgtcgttgg tgagaagcat
     2041 gaggccctcg gagttgaaat ccatttgccc gaccggtagc acccgttcac gcacggttcg
     2101 cagatagtcg gcgatgcacg ggcgaccttc ggggtctttt agagcggcga tgacggcttt
     2161 cggtttatag aaaaccaaat aaagtcgctc aacgtcggtg agaattaaac tgccgttaac
     2221 tttgatttta tctttatcgg gattggcttt agtgccgagc tcggtgactg tacgcccgtt
     2281 caccgtgacc gccccttcaa ggatcaaacg ctcagcttcg cggcgcgaac aaatgcccgc
     2341 gttggctata attttttgta accgttcgcc gctcgcggcg tgcgctgcga ttgtcgcggg
     2401 gtcgagcgcc gagttgacca ctggagctgg ttggtcaggg ttgtaagggg ttttcggggt
     2461 gctcacggaa taggcggttt ttttccaagg gcccttaaat gactttttct ttaattcgcg
     2521 ggacggacgg gcatttcgtt cctcacgccc tcctcggtga ggtcttccgt cgcgggggcc
     2581 cgcgccgtcg cgacgcggtc ggcgttccgc tgtagaagag tccgaaaatc tacgacgtga
     2641 atcgcccgtg cgagcgcgtg aatcgccagt gcgagcgcgt gaatcgccca tgcgagaccg
     2701 cgaatcgggc cgtgcgctag ttcgggaatc cgttcgtgaa ctcgatttcg agaatcgcgc
     2761 acctggtttg gccgtcgatt tttttcgaac aatggtgttt cttttgttgt cggtgcgagt
     2821 ggttctcatc ttgggattcc tgttcatttc gattgtcgat ataattgatt tattcgttgc
     2881 atgcgaagcc agtcatcgtc atcaaatgtg atatcgagtg cacgctcgtt taaatcttct
     2941 aaaactgttt ctttaggtaa
//
LOCUS       DAOMXO010000266         2959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18917_length_2959_cov_5.761364, whole genome shotgun sequence.
ACCESSION   DAOMXO010000266 DAOMXO010000000
VERSION     DAOMXO010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18917_length_2959_cov_5.761364"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLH57_06355"
     CDS             complement(<1..535)
                     /locus_tag="PLH57_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02271.1"
                     /translation="MLNGKLRLEGAIRNIFVYGAKGLACACAVVGVVFLAGLGSGCAP
                     LQKIPNLNIRALLDQQQIRTWQTASTIDAGDYEANNDTGRGCLDTGGGDIGLLNAGGG
                     ITRGCMQRPALAYSDGAWFVGFYQNYLFTTASSKFSAFVNSTLYGGVFGALGAGLNII
                     DTGLGVAPTAVAPMAGAR"
     gene            788..2500
                     /locus_tag="PLH57_06360"
     CDS             788..2500
                     /locus_tag="PLH57_06360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HRK02272.1"
                     /translation="MFSSRFAPILAFLLTIGLAPGSLSAATHVRSSQNGTIVKYIPGS
                     NVVFRANPSNASGLRAQEIFSIFSTSIERWIRASQGGLAFTYFQGTDPARYPTALAAP
                     QDNSIFFTSQASVNERLPCSVIGLTEVWYDLNSGNIRRSDMRLNDTCFRFSNNVADTR
                     MGSTTVYLGDVLTHELGHSFGLDHSQNLQSTMFFTAAKQMHKPSCDDQSAMVSNYALG
                     VAGNAGNVSGRVTANGSAVFGAYVSAIQIERGTVIASGLTERDGRFELTGLEPGTYTL
                     LVEPYYPGPATLNAHYSQISSSVCGGNFFQRSFLLDENGMLESINLGPGARADVGAIS
                     VSCLPPESPHFQLENSIATAPELDLSNNAVATMSVFGTSSDHYYRIDNASGTLSANAL
                     AYSLFSRTDVSLEWLDSAGRSISGQSISNNVFSAPSGYTNFDAKTVVTLGSPQTVFLH
                     VYTRGTIASHQFPASNLGVSNTPYYVVTIARDPAAPSVYPAGPRCEYNDSFGIYSQPG
                     EPNPYVSNFSAVEKETAAGCGSITNHSDNNRSGGMFRLVNFAVLLVALGILRRILSAN
                     ERSH"
     gene            2558..>2959
                     /locus_tag="PLH57_06365"
     CDS             2558..>2959
                     /locus_tag="PLH57_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRK02273.1"
                     /translation="MKRVMVVDDDNDILKFCDTLLAARGYESVTCSSALRAIELLRSH
                     PVDLIVVDIVMPIMDGFDLLKELKRMPQFRAPILVLTGRNATEDARRAISLGALDYMI
                     KPFDKDVFLAKVESLIGRASRSSLHGPEATHH"
BASE COUNT          677 a          847 c          711 g          724 t
ORIGIN      
        1 tacgggctcc cgccataggg gccaccgcgg taggagcaac accaagaccg gtgtcaataa
       61 tgttcaaacc ggctcccaag gccccgaaaa ctccgccgta gagtgttgaa ttcacgaatg
      121 cggaaaactt actcgaagca gtggtgaaaa gataattttg gtagaacccc acaaaccatg
      181 cgccatcgct gtaagcgagc gccggccgct gcatgcagcc tcgcgtaatg cccccccctg
      241 cattgagcag cccaatgtcg ccaccaccag tatctaaaca gccgcggccc gtgtcgttat
      301 tggcttcata atccccggcg tcgatagtgc tcgcggtttg ccaagtgcga atttgctgtt
      361 gatccagcag tgctcgaatg ttgaggttcg gaattttctg aagcggcgcg cacccactcc
      421 cgaggcctgc caagaaaacg acgcccacca ctgcgcaggc gcacgcaagg ccctttgccc
      481 cataaacgaa aatatttctt atcgcacctt ctaggcgcaa tttcccattc aacatagttc
      541 tccaagagaa cccatcggcc gaaatggtct gagagttggg ggtgtcatct tgcctaccct
      601 gtctttttta cccacccagt ctcagcgccc gctgcgggac aatggcccaa cacctcgcaa
      661 cttcagtgtt ttcggtcaga aatattttag gccccctctg gcacgagcct tgtaatgatt
      721 tagtatgaat aaacccaggc acggagcctg ggttcggtag cgcaaaccag tgggagttgg
      781 aacaaggatg ttctctagtc gctttgcccc tattttggct tttttgttaa cgatcgggtt
      841 ggctccaggc tcactttccg ccgccactca tgtgcgctcg tcccagaatg gcaccattgt
      901 aaaatatatt ccgggatcaa atgtggtttt tcgggctaat cctagcaatg cgagcggact
      961 tcgcgcgcaa gaaatttttt cgattttttc gacatctatt gaacgctgga tccgagcgag
     1021 ccagggcggc cttgcgttca cctactttca gggcaccgac ccagctcgtt accctacggc
     1081 acttgcggca ccacaagaca attctatttt ttttaccagt caagccagcg ttaacgaacg
     1141 ccttccctgt tccgtgatcg gacttaccga agtttggtat gatctcaact cgggcaacat
     1201 tcgtcgttcc gacatgcgcc tcaacgatac ctgttttcgt ttttccaaca acgtcgccga
     1261 cactcgtatg ggtagcacga cagtgtattt aggtgatgtg ctcactcacg aactcggcca
     1321 ttcattcggt ttagatcact cgcaaaatct gcaatcgacc atgtttttca ctgccgccaa
     1381 acaaatgcac aaaccatcgt gcgacgatca atctgccatg gtctcgaact acgcgctagg
     1441 agtggctggc aacgccggca atgttagtgg tcgcgtgact gccaatggtt ccgccgtgtt
     1501 tggcgcgtac gtcagtgcca ttcaaattga acgaggcacc gtgattgcta gtggactaac
     1561 cgaacgcgac ggacgattcg agcttacagg ccttgagccg ggaacttaca cactcttggt
     1621 cgaaccttac tacccgggac cagccaccct caacgcccac tattctcaga tttcttcgag
     1681 cgtgtgtggc ggaaattttt ttcaacgttc ttttctctta gacgaaaacg gcatgcttga
     1741 gtccataaac cttggaccgg gcgctcgcgc cgatgttgga gccatctcgg tttcctgcct
     1801 tccgcccgaa tctccacatt ttcaactcga gaactcaata gcaaccgcgc cagaactcga
     1861 tctatcgaac aacgcggtag ccaccatgag tgttttcgga actagcagtg atcactatta
     1921 ccgaatcgac aacgcgagcg gcaccttgag cgccaacgcg ctcgcttact cgttgttttc
     1981 acgcactgat gtcagccttg agtggttaga cagcgccggg cgcagcatct cggggcaatc
     2041 catctcgaac aatgttttca gcgcgccttc gggatacacc aatttcgatg cgaaaactgt
     2101 cgtcacgctc ggctcacccc aaaccgtatt cctacacgta tacactcgcg gaactattgc
     2161 gagtcatcag tttccagctt cgaatctagg cgtgtccaac acgccttact acgttgtcac
     2221 gattgcccgc gacccagcgg ctcctagcgt ctatccagca gggcctcgct gcgaatacaa
     2281 cgacagtttt ggaatctact cgcagcccgg tgagcccaat ccatatgttt cgaacttctc
     2341 agccgtagaa aaagaaacgg cggcgggctg cggatctatt acaaaccatt ccgacaacaa
     2401 ccgctctggc ggcatgtttc gcctggttaa ttttgccgta ctacttgtgg ccctcggaat
     2461 tctgcgccga atcctaagtg caaacgagcg aagccattaa ttttctgagt ttttataccg
     2521 atgcgggtct taggaacggg gggttcctga ctaaggtatg aaacgcgtaa tggtcgtaga
     2581 tgacgacaat gatattctaa aattttgcga cactttgctc gcggcccgcg ggtacgaatc
     2641 cgtaacttgt tcgagtgcgt tgcgagcaat tgagttgttg cgatcgcatc ccgtcgattt
     2701 aattgtcgtc gatatcgtta tgccaattat ggatggtttc gatctactca aagaactaaa
     2761 gcgcatgccc cagtttcgag ctccgatttt ggtgcttacc gggcgtaacg caacggaaga
     2821 cgcacgacgc gccatatcgt tgggtgcact cgactacatg attaagcctt tcgacaaaga
     2881 cgtttttctc gccaaagttg aatcgctaat tggtcgcgcg agtcgcagca gcctccacgg
     2941 gcccgaggct acgcaccac
//
LOCUS       DAOMXO010000267         2951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_18983_length_2951_cov_4.573204, whole genome shotgun sequence.
ACCESSION   DAOMXO010000267 DAOMXO010000000
VERSION     DAOMXO010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18983_length_2951_cov_4.573204"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PLH57_06370"
     CDS             complement(<1..872)
                     /locus_tag="PLH57_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02274.1"
                     /translation="MFDLNKIQTYSLRDRPSKVEVKDFAQIPSTDAVLAMNKWFDTLP
                     EILAAKDLRLLARAVVECRNRKKPIVWGMGSHVLKTGLAPLLIDLMNEGFITGLAFNG
                     SALIHDFETAFAGKTSEDVDSAIGDGDFGMARETGEIVNQEISKGVASGLGLGGSIGK
                     HIAESDYPYKDLSLFAHAYKCGLPATVHVAIGTDILHMHPKCDGAALGLGSHRDFLRF
                     AESVSLMEGGAYINFGSAVILPEVFLKAITLARNLGATVNQIVTANFDFINQYRGRVN
                     VVQRPVLAGGEGFE"
     gene            complement(856..1479)
                     /locus_tag="PLH57_06375"
     CDS             complement(856..1479)
                     /locus_tag="PLH57_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HRK02275.1"
                     /translation="MDPKQDSAKYLEHLDKTIQSSFKAKQHFYEKNRDQVIEAARWMG
                     ETVRAGGKMVIFGNGGSAADAQHMAAEMVGRMLVERKKPLPALALTTDTSALTAIGND
                     YSYEDVFELQVRALARKGDLAIAISTSGNSKNVVKAVRAAREMGVRVLSMTGGSGGML
                     RELSDLSLNVELGQNSSMIQETHITIIHLLVDLMDRFFLDENYVRLK"
     gene            1591..1920
                     /gene="trxA"
                     /locus_tag="PLH57_06380"
     CDS             1591..1920
                     /gene="trxA"
                     /locus_tag="PLH57_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRK02276.1"
                     /translation="MASKNVLAVTDGNFENEVIKSNVPVLVDFWAEWCGPCRALSPTI
                     DELADQYAGKVKITKVNVDENPEVPGRFRIRGIPTLLMFKNGQLVDQLVGAHPKGAID
                     DLIKKTL"
     gene            2089..2757
                     /locus_tag="PLH57_06385"
     CDS             2089..2757
                     /locus_tag="PLH57_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRK02277.1"
                     /translation="MEASNFIGSIILNFQKGGFFMYPIAITFAVAIAMVFERMMRLYV
                     QYNVDGTSFMFEVQKHVLANDLDGAIRVCNGASRTALARVIKAGLQRASRDETQIQNA
                     IDAASLEVIPKLERRLNYLALIANIATLLGLLGTVVGLIHSFAALEAADAAQRQAILA
                     KGISEAMNCTAFGLLVAITTMVMHSILAAKATSLLEEIDEFGVKLLDLLSARKYRHSS
                     EKPV"
     gene            2764..>2951
                     /locus_tag="PLH57_06390"
     CDS             2764..>2951
                     /locus_tag="PLH57_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02278.1"
                     /translation="MLKRPTSRRKSGSQEVNPNLVPMLDALVTLVAFLLFSSAFLSIV
                     VIDTPAPVLAPAEADLGK"
BASE COUNT          758 a          779 c          666 g          748 t
ORIGIN      
        1 atctcgaacc cctcgccccc ggcgagcacc gggcgctgca ccacgttcac gcgccctctg
       61 tattgattta tgaaatcgaa attggccgta acaatttggt ttactgtggc cccaagattc
      121 cgagcaagtg taatcgcttt taaaaacacc tcaggaagaa tcacggcgga accaaagttt
      181 atgtaggcgc caccttccat aagactcacg gactcagcga aacgtaaaaa atctcggtgg
      241 cttccgagcc ctaatgccgc tccatcacat ttcggatgca tgtgtagaat gtccgtaccg
      301 atagcgacgt gcacggtcgc cggaagcccg cacttgtaag cgtgcgcaaa caaagacaga
      361 tctttataag gataatcgga ctcagcaatg tgcttaccga tagagccacc cagccctaaa
      421 ccagaagcaa ccccctttga tatttcttga tttacaattt caccagtttc acgggccatt
      481 ccgaaatcgc cgtcaccaat cgcagaatca acatcttccg aagttttacc ggcaaaagcg
      541 gtttcgaaat cgtggatcaa tgccgaccca ttgaacgcaa gcccagtgat gaatccttcg
      601 ttcattaaat ctatcagcaa tggcgcgagc ccagttttta aaacatggga ccccatgccc
      661 caaacgattg gtttttttct atttcgacac tcgacgaccg cgcgggcgag taaccgcaga
      721 tccttggcag ctaagatctc ggggagagta tcaaaccatt tgttcatggc taatacggca
      781 tcggtcgatg gtatttgagc gaaatctttg acctcgactt tactcggacg atctcgaagg
      841 gaataagttt gaattttatt taagtcgaac atagttttcg tcgaggaaaa aacggtccat
      901 caggtccact aaaagatgaa taatagtaat gtgggtttct tgaatcatcg aagagttttg
      961 acctagctca acatttaaac ttagatcact taactctcga agcattccac cgcttccccc
     1021 cgtcatcgag aggacgcgaa cacccatttc acgagccgca cgaacggctt tgactacgtt
     1081 ttttgaattt ccactagtag aaatcgctat agcaagatcc cctttacgtg cgagtgcccg
     1141 tacttggagc tcaaacacgt cttcgtaact ataatcgttt cctatcgccg taagagcgct
     1201 cgtatccgtg gtcagcgcca gcgcaggcaa tggttttttg cgctcaacca gcattcggcc
     1261 caccatttcg gccgccatgt gctgagcatc ggcggcacta ccgccgttac caaaaatcac
     1321 cattttgccc ccggctctca ccgtctcccc catccagcgg gcagcctcaa tcacctgatc
     1381 acgatttttc tcataaaaat gttgtttagc cttaaaactg gactgtatcg ttttatctaa
     1441 atgttctagg tatttagctg aatcttgttt tgggtccatg gcgagattct ccgtttttgg
     1501 gggcgctcct ggcagttaga ggtttgccat atcctttttg atccacatat tttagaaagg
     1561 tagttcgaac gtaacatagg agatactcgc atggcttcaa aaaacgtact cgccgtcacc
     1621 gacggtaatt tcgaaaacga agtaatcaag tcaaacgttc cagtgttggt cgacttttgg
     1681 gccgaatggt gtggcccatg cagagcattg tctccaacga tcgatgaact ggccgatcag
     1741 tacgctggca aagttaaaat caccaaagta aacgtcgacg aaaacccgga agtgcccgga
     1801 cgttttcgca ttcgcggcat tcctacgctg ttgatgttta aaaacggtca attagttgat
     1861 cagctcgtag gggcgcaccc taagggcgcg atcgacgacc ttattaaaaa gacgctgtaa
     1921 gccaaaataa cgcctcgcgt cttttaaaaa taacttcgtg atttgaccgg caaacgtccg
     1981 atccagtttg atatccttga cggaaaaccg gaccatgtta cccttataat aagtagcatg
     2041 gacggctgct cgcatcgcta acgctgcggg accatcagag gagacagtat ggaagcgtcg
     2101 aacttcatcg ggtcaataat acttaatttt caaaagggcg gttttttcat gtatccgatc
     2161 gcgatcacct tcgcggtcgc tatcgccatg gtcttcgagc gcatgatgcg cctctatgtc
     2221 caatacaacg tagacggcac ttcgttcatg ttcgaagtgc aaaaacatgt tctcgcaaac
     2281 gatctcgacg gcgccattcg agtttgcaac ggagcaagcc gaaccgcctt ggctcgagtg
     2341 ataaaagccg gtctacagcg cgccagtcgc gacgaaactc agattcaaaa cgcgatcgac
     2401 gcagccagcc ttgaagtcat tccgaaactc gagcgaagac tcaactactt ggctcttatt
     2461 gctaacatcg ctacgcttct cggacttctc ggtacggtcg tgggtcttat tcacagtttc
     2521 gcagctctcg aagctgccga tgccgctcaa cgccaagcta ttcttgctaa aggtatttcc
     2581 gaagcaatga actgtaccgc gttcggtcta ctggtagcaa tcacgacgat ggtcatgcac
     2641 agtatactgg ctgccaaagc tacttcgctt ctcgaagaaa tcgacgaatt cggggttaaa
     2701 ctgctcgacc tgttatcggc ccgaaagtac cgtcacagct ctgaaaaacc ggtgtaatag
     2761 caaatgttga agcgaccgac gtcacgccgc aaatctggat cgcaggaggt taatcccaac
     2821 ctcgtcccca tgcttgatgc tttagttacg ttggtggcgt tcctcctttt ttcgagtgca
     2881 tttctttcga tcgttgtcat cgatacgccg gcgccagttc tcgctccggc cgaagctgac
     2941 ttgggaaaaa t
//
LOCUS       DAOMXO010000268         2940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19093_length_2940_cov_4.089428, whole genome shotgun sequence.
ACCESSION   DAOMXO010000268 DAOMXO010000000
VERSION     DAOMXO010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19093_length_2940_cov_4.089428"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLH57_06395"
     CDS             <1..732
                     /locus_tag="PLH57_06395"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRK02279.1"
                     /translation="QSMPSEAIERIWGEGRVRIFLSHKAEFKEQTAGLKAALLRCGIV
                     SFVAHEDIEPAEEWQPEIERALFSMDALVALLTANFHDSNWTDQEVGVAIGRGIPVIG
                     IQLGCVPYGLLGKSQALGGCTLSRPEEMAAAIFGVLYRRLPDKSRLFDAALTAYSAST
                     TWHDSAWKVQNLFTRFESLSEEQVNRLMAAYNTNVQNSNSFDGMDALNPLLNKWTGKT
                     WIVQNNRLVQSKPLSQPSQDDEIPF"
     gene            1101..1775
                     /locus_tag="PLH57_06400"
     CDS             1101..1775
                     /locus_tag="PLH57_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HRK02280.1"
                     /translation="MFNHSGANEVVISNVRKAIREVFGSNSPGFAEHQYVNLYAGSLY
                     MGMPDHEYLKGLEFGRQKLIVKLNGLIDRLKEKKEDLIGGSTFSSPSIDFDRLDVHPR
                     ISDVSRELFSDGHPWEAVFAASKALVNFVKERSGRYDLDGANLARMVFSRNNPLLAFN
                     DMSDTTDRDEQEGMMHLFECVILGIRKPGGHSFPEWSELRALEYISLISLLAYRVQEA
                     KRIKQP"
     gene            1862..>2940
                     /locus_tag="PLH57_06405"
     CDS             1862..>2940
                     /locus_tag="PLH57_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02281.1"
                     /translation="MNKKYPLVASDAFLSQNIDTLNKYSAVEEFADALGDGNDKNLRG
                     IEYHLIQLRNDLRKRLVAKQIFIGTSIIEEVIFNCARNGASDSVNAAFKLINSSVVAS
                     PGLIIYPLNSFGILGVAGCVLRGEEFPTFVLPDCGIALIPQQNSIEKVKRRISEMVSE
                     LGVRAKLDERLFEHYHVSRDLKWLENNPLLFVKVRQFQDYFHENQFFVVRKTLFARLL
                     VYMLHVLQDDPDTDVEPLWSTSRVNNWETLDCKHYLLLQRDPRSASKLSVNCVPMNID
                     RAELTEMCELNANINRQAWQRKEKRFNQVVLAVKQFEKIYFGNIFRTNKNNQWRRLLE
                     RLERSIRYFTRSFRNSSLTFDSNVYL"
BASE COUNT          819 a          635 c          721 g          765 t
ORIGIN      
        1 caatctatgc cgagtgaggc aatagaacgt atatggggag agggccgcgt cagaatcttc
       61 ttaagtcaca aggcggaatt taaagagcaa acggcaggac tcaaagccgc attgctccgt
      121 tgcggtatcg tttcatttgt cgcgcatgaa gatatcgaac cagccgagga gtggcagcca
      181 gagattgagc gggccctttt cagtatggac gcactcgtgg cgcttctcac tgcaaacttt
      241 cacgacagta actggactga tcaagaagtc ggtgtcgcga taggccgagg tatacctgtg
      301 attggaatcc aacttggatg tgtaccgtac ggactactcg ggaaatctca agctctcggt
      361 ggctgtactt tgtctagacc cgaggagatg gcggcagcga tattcggagt cctatataga
      421 cgcctaccag acaaatcgcg cctatttgac gctgcgctta cggcatatag cgcttccacc
      481 acatggcatg actccgcttg gaaagtgcag aacttattta cgcgattcga gtccctttcc
      541 gaggaacaag ttaacagatt aatggcggca tacaatacga acgtccaaaa tagtaactct
      601 tttgacggta tggatgcgtt gaatccgctc ttgaacaaat ggactggaaa gacttggatc
      661 gtccagaaca atcgcctcgt tcaatccaaa cctttgtctc agccttcaca agacgacgaa
      721 atccccttct aaagcgaaat tgcccaaggt gaacaaaact aagctcatcc attgggaaaa
      781 cctatcgaat acaactcgtc cgatgcatca gcaacgacaa ttaccccgcc ctcggggcat
      841 tcgaaaacgc agtatagcaa cggttatcta agaaactgta tgtaccaagg cgggccctac
      901 gtccttgaag tttgagcacg tggacggtaa gttgaatact gttcaattct atcgctagga
      961 ggtagactaa tggcaaagaa acgccccgaa cctatactcg tagagcaaaa gaattccgaa
     1021 gtgaggcaga aatctacatc ggaatatcta agctggaacg aagaattcgc gaggtagagc
     1081 aactcgacct cccgggggta gtgtttaatc attcaggcgc taacgaagtc gtaattagca
     1141 acgtgcgtaa ggcaattcgg gaagtattcg gctcgaactc gccaggattt gcggaacacc
     1201 agtatgtaaa cctttatgct ggttctcttt atatgggtat gccggaccac gagtacctta
     1261 aaggcttgga attcggccga cagaagctaa ttgttaaatt gaatgggctt atagatagat
     1321 taaaagagaa gaaggaagac ctaatcggtg ggtcgacatt ttcttcgcca tctatcgatt
     1381 tcgatcgctt ggatgtacac cctaggattt ccgatgtctc gcgtgaactt ttttcggatg
     1441 ggcatccttg ggaggcagtc tttgcggcct cgaaggcatt agtaaatttc gtgaaggaac
     1501 gttccggtag gtatgacctc gacggtgcga acttggctag aatggtcttc tctagaaata
     1561 atccgctgct cgcgtttaac gacatgagcg acactacgga tcgcgatgaa caagaaggaa
     1621 tgatgcatct cttcgaatgt gttatcttgg ggattcggaa gccgggtggt cattcctttc
     1681 ctgagtggtc tgaactgcgg gctctcgagt atatttctct gataagtctc cttgcctaca
     1741 gagtgcaaga agctaaacgc ataaagcaac cgtagtttgt ttgaaaattg accgtcaatt
     1801 aaacgagacg caaaagtatg aagagcatag ttgagctgca agtagcatgt ggcgtttgtc
     1861 aatgaataag aaataccctt tggtggcgtc cgacgccttt ctttcccaaa atatcgatac
     1921 cctaaacaag tattcggcgg tggaagagtt cgctgatgct ctgggggatg gcaacgacaa
     1981 gaatcttcga ggcatagaat atcatttgat tcagttaaga aatgatcttc gaaagcgctt
     2041 ggttgcgaag caaatattta tcggcacatc aatcattgaa gaagttatct ttaattgcgc
     2101 aagaaatggc gcgtccgatt ctgtgaatgc ggcgtttaaa ctaataaatt cttccgtagt
     2161 cgctagtccc gggctaatta tataccctct taactcgttt ggaattcttg gcgttgcggg
     2221 gtgcgtgcta agaggtgagg aatttccaac tttcgtcctt cctgactgcg ggatcgcact
     2281 gattcctcag caaaattcta tcgagaaggt aaaacggcga atttccgaga tggtatcgga
     2341 gttgggtgta cgcgctaaac ttgatgagcg cctctttgag cactatcatg tatcgcgcga
     2401 cctcaaatgg ttggaaaata atccactgct tttcgtaaaa gtgcggcagt ttcaagatta
     2461 tttccacgag aatcagtttt ttgtcgtaag aaaaacgctt tttgctcggt tgttggtgta
     2521 tatgttgcac gttttgcagg atgaccccga cactgacgtt gagccgttgt ggagtacttc
     2581 gcgagtaaac aattgggaaa cgttagactg caagcattac ctgctgctgc agcgcgatcc
     2641 gagatcagct tcaaaacttt cggtaaactg cgttcctatg aatatcgatc gagctgagtt
     2701 gacggaaatg tgcgaactta acgcgaacat taaccgtcaa gcatggcaaa gaaaagaaaa
     2761 gcgattcaat caagtcgttt tagcggttaa gcaatttgag aaaatttatt ttggcaacat
     2821 ctttcgcact aataaaaaca atcaatggag gcgattgctt gaaagactag agagatcgat
     2881 cagatacttt actcgttcat ttcggaattc atccctcact tttgacagca acgtatatct
//
LOCUS       DAOMXO010000269         2927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19207_length_2927_cov_4.308496, whole genome shotgun sequence.
ACCESSION   DAOMXO010000269 DAOMXO010000000
VERSION     DAOMXO010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19207_length_2927_cov_4.308496"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLH57_06410"
     CDS             complement(<1..227)
                     /locus_tag="PLH57_06410"
                     /inference="COORDINATES: protein motif:HMM:NF016279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaG domain"
                     /protein_id="HRK02282.1"
                     /translation="MNYTAVTHDIEVTARPIFERRQSVPFTPVYTWSYEIQITNRSER
                     VVQLLYRYWKISDAAGGVKEVRGAGVIGQHP"
     gene            complement(224..538)
                     /locus_tag="PLH57_06415"
     CDS             complement(224..538)
                     /locus_tag="PLH57_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRK02283.1"
                     /translation="MTEAFNKYDVHLFVCTNDRKTGASCGAKGGGSLREAVKNKCRDF
                     GENPQHRIRVNASGCLGHCEKGIVAVSYPQGQWHFELENDAKAVDQLTESVRRLITSP
                     KL"
     gene            complement(531..1634)
                     /locus_tag="PLH57_06420"
     CDS             complement(531..1634)
                     /locus_tag="PLH57_06420"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRK02284.1"
                     /translation="MHKTEFLTADSTGIERAAQLLLGSELVGIPTETVYGLAALAFDE
                     TAVLKIFRAKERPLFDPLIVHLPATWNSVNALGEAGVINPDRLSPQARTVTETLMEKF
                     WPGPLTLVLPRGHRIKDLVTSGLGTVGLRVPRQAIALKLLERVNEPLAAPSANRFGRI
                     SPTLAAHVMTELEGRISAVLDGGPSQVGVESTIVAIDPKTGDLVCLRPGGVAMESLEL
                     ALKKLGTFKLTQNSTANEKQNTPLPAPGLLLSHYAPTTKLLLQKRSELELGLRDGFAK
                     FPQKKIGVLLLSGPSVADPRCERLCVLSEKNDESEMAQKLFATLRELDVLGLDLILAE
                     YCDKESGLLAAIQDRLLRAAGKSKNSLGSGNND"
     gene            1672..2925
                     /locus_tag="PLH57_06425"
     CDS             1672..2925
                     /locus_tag="PLH57_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRK02285.1"
                     /translation="MATPPSQPVLGIIGEGQLARMLVEAAERRGISTCVWSSLPHSPV
                     SHLGAAHCEITASLDSLLTKATVVAFENEFQDTAMLRAAAQKVGRDPEQLFIPKLSAI
                     QLLQDKLEQKNICTRLGLPTSRFSAAPSAFNTSDEAFAWATAASRQFPRGFVFKWSRL
                     GYDGKGVLVLKEDENIDRADVRQIAFFEAAISTKALVYVEEYVPFTSECALVSAHQPG
                     PGDPVARYFPLVLTEQTQGICTRARGPMKAGLSSILEASARKAADALAKDLGLQGVFA
                     IEFFVVPGSNKLWINEIAPRVHNSGHLTQDAFDSSQFDFHVLAALGRLHESTAPKTRT
                     PHYGMLNILGPAAMQNSLPITDAIPSISDSVGRLHWYGKSEVKAGRKLGHINLTAGSI
                     PELDDKFREAEAFVRNLHSHWSSHG"
BASE COUNT          671 a          805 c          720 g          731 t
ORIGIN      
        1 attgggtgtt gtccaatgac tccggcgcca cgcacttctt tgacaccccc agcggcgtca
       61 ctaattttcc aatagcgata aagaagttgc accacgcgtt ccgaacgatt ggttatctga
      121 atttcgtagg accaagtata aaccggagta aagggtacag actggcggcg ttcaaaaatt
      181 ggccgagctg ttacttcgat gtcgtgggtg acggcggtat agttcataat tttggtgacg
      241 ttattagtct tcgcacactc tcggtgagtt gatcaactgc tttagcgtcg ttctctagtt
      301 cgaaatgcca ctgcccctgg gggtacgaaa ccgccacgat ccctttttcg cagtggccta
      361 aacaacccga cgcattcacc cgaatccgat gttgtggatt ttctccgaaa tctcggcact
      421 tgtttttaac agcctcgcgc agagatcctc cacccttggc gccacaggac gctccggttt
      481 ttcgatcgtt ggtgcagaca aaaagatgaa catcgtattt gttaaacgcc tcagtcattg
      541 tttccactcc ccagagagtt cttcgacttg ccggcggctc gcaacagtcg gtcctgaatt
      601 gcggccaaca ggcctgattc cttgtcgcaa tactcagcta aaattagatc cagccccaat
      661 acgtcaagct cgcgtagcgt cgcgaagagt ttttgggcca tttccgattc atcatttttt
      721 tctgaaagca cgcatagccg ttcacatcta ggatccgcga ctgacggccc tgaaagcagt
      781 agcactccaa tttttttttg tgggaatttc gcaaacccgt cgcgaagccc taattctaat
      841 tcactgcgtt tttggagcag aagttttgtg gtcggcgcgt agtgactaag aagtaggccg
      901 ggcgccggta aaggtgtatt ttgtttctcg tttgccgttg agttctgagt taacttaaat
      961 gttcccaatt tttttagtgc tagctcgaga ctctccatgg ccactccacc cggccgaagg
     1021 cacactaggt ctcctgtttt cggatctatc gccacaatcg tcgattccac gccaacttgc
     1081 gacggcccac cgtcgagcac cgccgagatt cggccttcta attcggtcat cacatgggct
     1141 gccaacgttg gactaattcg cccgaagcgg tttgcactcg gggctgcgag aggctcgttt
     1201 acacgctcca aaagtttcaa cgcgatcgcc tggcgtggca cccgcaagcc aaccgtgcca
     1261 aggccactag taactagatc tttaattcga tgcccgcgcg gtaaaaccaa cgtcaatgga
     1321 ccaggccaga acttctccat cagcgtttcg gtcacggtac gagcctgtgg tgaaagacgg
     1381 tccggattaa taaccccagc ttcgcccagg gcattgaccg aattccaggt cgccggcagg
     1441 tgtacgatca gggggtcaaa aagcggccgc tctttggcgc ggaatatttt taacacggct
     1501 gtttcatcaa aggccagggc agcgagtccg taaactgttt ccgtgggaat acccacgagc
     1561 tcagagccga ggagcagctg agcggctcgt tcgatgccag tggaatccgc ggttaaaaac
     1621 tcggtcttgt gcatatagcc gctttaccat agtttgttag aagagtctcg aatggcaaca
     1681 ccaccgtcac aacccgtgct tggcatcata ggagaagggc aattagctcg aatgctcgtc
     1741 gaagcggccg agcgtcgtgg gatttcaact tgtgtctgga gttcgctccc tcactcaccc
     1801 gttagccacc taggcgccgc ccattgtgaa ataaccgctt cattggactc gcttcttacc
     1861 aaagccaccg ttgtggcttt tgaaaatgaa tttcaagaca ccgccatgct gcgggcggcg
     1921 gctcaaaagg tcggacgtga ccctgaacaa cttttcattc caaaactttc ggcaattcag
     1981 ctgttgcagg acaaactgga acaaaaaaac atctgcactc gcctcggttt gccgacatcg
     2041 cgtttttcag cggcaccctc tgcatttaac acctctgatg aggccttcgc ttgggcaact
     2101 gcggcctcaa ggcagttccc ccgcggattt gtttttaagt ggagtcgtct cgggtacgac
     2161 ggcaaaggcg tcctggtttt aaaggaagac gaaaacatcg accgcgccga tgttcgacaa
     2221 attgcatttt ttgaagccgc cattagcacc aaggcgctcg tttacgtcga agaatatgtg
     2281 ccctttacca gcgagtgtgc gctcgtgagt gctcatcagc ccgggcctgg tgatcctgtt
     2341 gctcgttatt ttcctttagt actaacggaa caaacacaag gcatctgtac ccgagctcgg
     2401 ggcccaatga aggcgggttt gtcttccatt ctcgaagcaa gcgcacgcaa agccgctgac
     2461 gcactagcaa aagatcttgg tcttcaaggg gtgtttgcca tcgaattttt cgtagtaccg
     2521 ggttccaaca aactctggat caatgaaata gctcctcgcg tccacaattc gggccacctg
     2581 acccaagacg ctttcgactc tagtcagttt gattttcacg ttttggctgc gctgggtcga
     2641 ctgcacgaaa gcacggcccc gaaaactcgg actcctcact acggaatgct caacattctg
     2701 ggccctgcgg caatgcaaaa ttctttgcca atcaccgacg ctattccttc gatttccgac
     2761 tccgtgggcc gactccactg gtacggcaaa agcgaagtta aagctgggcg aaaacttggc
     2821 cacatcaacc ttaccgcggg ttcgatacct gaacttgatg acaaattccg agaggccgaa
     2881 gcattcgttc gtaatcttca ttcacactgg agttcgcatg gctaaac
//
LOCUS       DAOMXO010000270         2927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19208_length_2927_cov_4.219359, whole genome shotgun sequence.
ACCESSION   DAOMXO010000270 DAOMXO010000000
VERSION     DAOMXO010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19208_length_2927_cov_4.219359"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLH57_06430"
     CDS             complement(<1..295)
                     /locus_tag="PLH57_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017313578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HRK02286.1"
                     /translation="MKNMNTIDIGIPEGDRKAIAEGLSRLLADSYTLYLKTHNFHWNV
                     TGPMFQTLHLMFEAQYTELSTAVDLIAERIRALGFPAPATYSEFLKLTSIKEPI"
     gene            complement(326..853)
                     /locus_tag="PLH57_06435"
     CDS             complement(326..853)
                     /locus_tag="PLH57_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013300291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRK02287.1"
                     /translation="MSISLLRDSLKDYARDIKLNLGSVLTEEGAPGLTKSQILGTALS
                     CAYATKNSKVIEALEGDVRSVVAPETLESAKAATTIMAMNNVYYRTVHLSEDKDLRSL
                     PARLRMNVIGKPGIAKVDFELMCLAVSAMSGCGACINSHISEVKKAGISTEGAQSAIR
                     IASVINALAQAATIG"
     gene            complement(866..1402)
                     /locus_tag="PLH57_06440"
     CDS             complement(866..1402)
                     /locus_tag="PLH57_06440"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRK02288.1"
                     /translation="MLGIGKKFPEFKLKAVVSTEAKNAFKDVTNKTFAGKWLVVFFYP
                     KDFTFVCPTEIKGFADLHSEFKERDTEVLAASTDSEFVHLAWRQSHGDLKNLPFPMLS
                     DIKRELSGSLGILDENEGVAQRATFIVDPQGIIRFVYVTDLSVGRNPNEVLRILDGLQ
                     TDELCPCNWEKGEETIKV"
     gene            1523..2476
                     /locus_tag="PLH57_06445"
     CDS             1523..2476
                     /locus_tag="PLH57_06445"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HRK02289.1"
                     /translation="MPTITQLQYIVTVDKLRHFGEAAQACHVSQPSLSMQIMKVEDEI
                     GFPLFDRVKKPIVPTEKGARFIESARAVLREHERMMAHAKKDEKEVSGEFRLGVIPTI
                     APYLLPLFLEKFSITYPKVKLHVDEMKTESIVAELKKDLLDAAVLATPLNEQGLNEEP
                     MFYEKFYLYVSENHPLSKKTRVKQDDIDGTGIWLIQDGHCLRNQMIRLCSLKNQKGVF
                     KNIYFEGGNLETLRYLVQKGHGYTLIPQLFLNTLSPPERKKYVREFETPSPMREVSLI
                     ARRDQWKSDIMRAIFDSITQNLPKDFQTSLAAKEALVVKVV"
     gene            2473..>2927
                     /locus_tag="PLH57_06450"
     CDS             2473..>2927
                     /locus_tag="PLH57_06450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRK02290.1"
                     /translation="MKKLSTVFIRDEWLLLGVALFIYFAGIWDQSMPTPDGAHRASMA
                     REVLERATWFPIYYQGHALADHPPLYVWLVALVFKLFGVGDLQFIMVNRVLVTIGVLL
                     LYFAGKSIFEKKGQALFACLMLLVTVGFYQGSVAGNIEPLLNIFLIGAF"
BASE COUNT          772 a          649 c          673 g          833 t
ORIGIN      
        1 caatcggttc tttaattgaa gtgagcttta aaaactcgct gtacgttgcg ggagccggaa
       61 accctagagc gcgaattcgt tcggcgatca agtccacagc ggtggatagc tcagtatact
      121 gggcttcgaa cattaagtgc aatgtttgga acattggtcc cgtgacgttc caatgaaaat
      181 tgtgagtctt caaataaagg gtgtaagaat ctgctagtag gcgagagaga ccctctgcga
      241 ttgctttgcg gtcaccctcg ggtatcccaa tgtcgatcgt attcatgttt ttcataagaa
      301 accttccttt attgcttccc cgactctagc cgatcgttgc ggcctgcgcc agggcattga
      361 taaccgaggc gattcggatc gccgactggg cgccttcggt tgaaattcca gcctttttta
      421 cttcggatat atgcgaattg atgcacgcgc cgcagccact catggcagat acggccaagc
      481 acattagttc gaagtcgact ttggctatac ccggttttcc aataacgttc atacgaagac
      541 gcgccggcag acttcgcagg tctttgtctt cacttaggtg aacggtgcga tagtagacgt
      601 tgttcatagc catgatcgtg gttgcggctt tggcagattc tagggtttct ggggcaacaa
      661 ccgaacgaac gtcaccctcc agcgcttcaa tgacttttga attttttgtc gcataggcgc
      721 aagaaagggc agtgcccagg atttgactct tggtcaagcc cggtgcgccc tcctccgtga
      781 gaacgcttcc gagattgagt ttgatgtcgc gggcatagtc tttaagggag tcccttaata
      841 acgagatgct catagtggtt gctccttaaa ccttgatagt ttcttcgcct ttttcccagt
      901 tgcaggggca aagttcgtcg gtttgcaagc catccaaaat tcggagcact tcgttgggat
      961 ttcttccaac gcttaaatcc gtaacgtaga caaaacgaat aatgccttgg gggtctacaa
     1021 taaaagtagc acgttgtgcc acgccctcgt tttcgtccaa aattccgagg ctaccggata
     1081 gttcacgttt tatatccgaa agcattggga agggtaagtt cttcaaatca ccatggctct
     1141 gtcgccaagc taaatggacg aactccgaat cggtgctagc ggccaatact tcggtgtcgc
     1201 gttccttgaa ttcggaatga agatccgcaa accccttaat ttcggtagga cacacaaatg
     1261 taaagtcttt cgggtagaag aacacaacca accactttcc agcgaaagtt ttattggtta
     1321 cgtctttaaa ggcattcttt gcttcagttg agacaactgc tttcaattta aattctggaa
     1381 acttctttcc tattcctaac ataacgtgat cctccttatt tttaagatcg ttatgttttg
     1441 attctcggcc tttttgtgtc atatgtataa tatatcgttt ttatcatatg atagataaaa
     1501 actattacga gagaggtgcg atttgcccac aatcactcaa ctgcaatata tcgtaacagt
     1561 cgataaatta aggcatttcg gcgaagcggc tcaggcctgt catgtctcgc aaccaagttt
     1621 aagcatgcag attatgaaag tagaagacga gattggtttc ccgctgtttg atcgggtgaa
     1681 gaaacccatt gtgccgaccg aaaaaggagc gcgctttatt gagtcagcca gggcagtgct
     1741 tcgggagcac gagcgcatga tggcccatgc caaaaaagac gaaaaggagg tgagtggaga
     1801 atttcggctt ggagtaattc ctactattgc tccgtatcta ttgccgctat tcctcgaaaa
     1861 gttttcgata acctacccta aggtcaaact acatgtagat gaaatgaaga ccgagtctat
     1921 agtggctgaa ttgaaaaaag atcttcttga tgcagcggta ttggccaccc cgttaaatga
     1981 acagggttta aatgaagaac caatgtttta cgaaaaattc tatttatacg tttcagaaaa
     2041 ccacccactc tcaaaaaaaa ctcgcgtcaa gcaagacgat atcgacggaa ccggaatttg
     2101 gttaattcaa gacgggcatt gcttaagaaa tcaaatgatt cgcttatgtt ctctaaaaaa
     2161 tcaaaaggga gtattcaaaa atatttattt tgaaggtgga aatctagaga cactccgata
     2221 ccttgttcaa aaaggacacg ggtacaccct aattccacaa ttgtttttaa ataccctgtc
     2281 acctcctgaa cggaaaaaat atgtacgcga atttgaaact ccatctccca tgcgcgaagt
     2341 cagtcttatt gctcgacgag accaatggaa gtcagacatc atgagagcaa tttttgattc
     2401 tattacgcag aatttaccaa aagattttca gacttcgttg gctgccaaag aagccttagt
     2461 tgtgaaggtt gtgtgaaaaa actttccacc gtttttatta gagatgagtg gctcctctta
     2521 ggggtcgcct tattcattta tttcgctggg atatgggatc aatccatgcc gacgcccgat
     2581 ggtgctcatc gcgcgagcat ggcgcgcgag gtcctagaac gggccacctg gttccccatc
     2641 tactatcagg gacacgccct ggctgatcat ccaccgctat atgtgtggct tgtcgcctta
     2701 gtttttaaac tcttcggggt tggagatctg caattcatca tggtgaatcg agttctggta
     2761 accattggag tcttgctgct gtatttcgct ggaaagtcga tttttgaaaa aaaaggccaa
     2821 gctctttttg cttgtttaat gttgctggtg acggtcggat tttaccaagg ttcggtcgcc
     2881 ggaaacatcg agcctcttct gaatatattt ttaatcggcg cattttt
//
LOCUS       DAOMXO010000271         2923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19239_length_2923_cov_6.131450, whole genome shotgun sequence.
ACCESSION   DAOMXO010000271 DAOMXO010000000
VERSION     DAOMXO010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19239_length_2923_cov_6.131450"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2251)
                     /locus_tag="PLH57_06455"
     CDS             complement(<1..2251)
                     /locus_tag="PLH57_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRK02291.1"
                     /translation="MLSLAGILAGGCSPKLLRTLPVLTAAATSVSDSGGGGGGSSPSL
                     TISAAGLRGLQAATGDFDQDGDVDVVFVSQTTDAVLYAAGDGSGGFAAVVSYAMAQTP
                     VGVTAGDLDRDGDLDLFVTSDTSNVYYIPGNGDGTFGAASSNFAWSANIGITMGNFDT
                     DGDLDAVVTLIGSDQVAPLMGSFGGTLTFGATYAVGDMPTGIASGVLDLNGTANDGKL
                     SIVVANSGANTISVLLGNGNGTFNAAVSYAMGTTPVGVALGDFNKDGALDVVTANSGS
                     SNISIRTGVGDGTFGAETTYTVGTNPVGVVVADFNRDGDLDIAVTNNGSDNISVLLGA
                     AGATFGAATNTATSDGPMAIVAADFNRNGEPDAVAIESTDNVPTLVSGTDPADPATVT
                     QASTIPGSTQCDAALGDIDHDGDIDIVYSNLGSGTVGVYDNNGSGAFALVENLNVGSG
                     AGSQPCGIELTDFTRDGWLDFVVTLRTDGTVQTFTNDRDGTFTLADTQNFLAGAGTPW
                     DVEVGDMDKDGDPDVVVIGIETGDANSAVEVFFNNSAAVLSTYDSVIDGPNDTWRGSL
                     ADLDNDGNLDISVATRTVADLTAALNDGSGGFPVAFTTVLNEAVGNDIIDVITADINR
                     DGWLDFFIANTATAGAQDRELQYVRGDGTGALGSFLAPLNYDVTGADGNATAVLSGDF
                     DWDGDLDVAIALTRDASVEVMTSDLTTGFTGFTTTDTEINCTDSGWGDVADLNGDGAL
                     DFALTCSTDN"
     gene            complement(2435..>2923)
                     /locus_tag="PLH57_06460"
     CDS             complement(2435..>2923)
                     /locus_tag="PLH57_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02292.1"
                     /translation="SSAAHEIAPLAKSSKDLAKGLEAVLARGASHAVGKSKLANVQHQ
                     PTVTKVELKANIDLHSQAYVEASDLHVQIANMNSANIGEVQRQFEAWSVRRVPVELKM
                     RLLEDFLALHPQIRGVTSVAVYELVRSELALLEGDSTVLSERRHRSQLEQLRRLFENT
                     FR"
BASE COUNT          738 a          828 c          692 g          665 t
ORIGIN      
        1 tattatcggt tgaacacgtg agcgcaaaat ccaacgcgcc gtctccgtta agatccgcga
       61 catcacccca gccagaatca gtgcagttaa tttcggtgtc ggtggtagta aaaccagtaa
      121 atccagtggt taagtcactg gtcattacct caacggaagc atcgcgagta agggcaattg
      181 cgacgtcgag gtcgccgtcc caatcgaaat caccggacaa tactgcggta gcgttgccgt
      241 ccgcgcctgt aacgtcatag ttaaggggag ccaaaaaaga acccaacgca cctgttccgt
      301 cgccccgaac gtactgcaac tccctgtcct gcgcacctgc agtcgctgtg ttggcgatga
      361 aaaagtctaa ccaaccgtca cggttaatgt ccgcagtgat gacgtcgatt atatcgttgc
      421 cgacggcttc gttcaaaaca gttgtgaatg caaccggaaa tcccccggag ccgtcgttga
      481 gcgccgcggt aagatcagcc acagtgcgag ttgcgaccga aatgtcgagg tttccgtcat
      541 tgtccaaatc ggcaagactt cctctccagg tatcgttcgg cccgtcgata acagaatcat
      601 acgtcgagag aactgcagca ctgttattaa agaatacttc tacggccgaa ttggcgtctc
      661 cggtttcaat gcctatgacc accacatctg ggtcgccgtc tttatccatg tctcccacct
      721 cgacgtccca aggcgtaccc gcgcctgcta aaaaattttg ggtatcggcc agagtaaacg
      781 tgccatcgcg gtcattggta aaagtttgca cagtgccgtc ggtacgaagg gtgactacga
      841 agtctagcca cccatctcga gtaaaatctg tgagttcaat accgcagggt tgggaccccg
      901 caccggagcc tacgtttaga ttttcgacca aagcaaacgc ccctgaaccg ttgttgtcgt
      961 aaactccgac cgtgccgcta cctaaatttg aataaacaat gtcgatgtcg ccgtcatgat
     1021 cgatgtcgcc gagtgcggca tcgcactgcg tgctcccggg tatggtgctc gcttgcgtta
     1081 ctgtggctgg atcggcaggg tcggtacctg aaaccaaagt tggtacgttg tcggtcgatt
     1141 caatggccac agcgtcgggc tccccgttgc ggttaaaatc ggccgccacg attgccattg
     1201 gcccgtcact cgttgcagtg ttggtggcgg cgccaaaggt tgcgcccgca gcgccaagca
     1261 aaacactgat gttatctgaa ccgttattag ttaccgcgat atctaaatca ccatctcgat
     1321 taaagtcggc aaccaccacg ccaaccggat tggtgcccac ggtgtaagtg gtttcggcac
     1381 cgaaagttcc atctccaact ccagtgcgaa tagaaatatt cgaactgccg gaattggccg
     1441 tcactacgtc aagtgctcca tctttattaa aatcgccgag cgcgacgccc accggagtcg
     1501 tccccatagc gtagctcacc gcggcgttaa aggttccatt accattaccc agcaggaccg
     1561 aaattgtatt cgctcccgaa ttggcaacta cgattgaaag ctttccgtcg ttcgcagttc
     1621 cattcaggtc taaaaccccc gaagcaatac ccgttggcat atcaccgacc gcgtaggtcg
     1681 caccaaaggt aagcgttcca ccgaaactgc ccatcaaggg ggccacctga tcagaaccta
     1741 tgagagtgac caccgcgtcc agatcgccat cagtgtcgaa gttgcccatg gtgattccaa
     1801 tgttcgctga ccacgcaaaa ttgctcgaag ccgcgccaaa ggtaccgtct ccgtttcctg
     1861 gaatgtaata aacgttgctg gtatcgctgg ttacgaaaag atccagatca ccgtcacgat
     1921 ctaagtcacc cgccgttacg ccaacgggag tttgagccat cgcatacgac actacggcag
     1981 caaagccgcc actgccgtcg ccagctgcgt ataggaccgc atcggtggtc tgactaacaa
     2041 atacgacatc gacatcgccg tcttggtcaa aatcaccagt ggctgcctgc aatccgcgca
     2101 aaccagcagc cgaaattgtg aggctaggac tacttccgcc tccaccaccg ccgctatcac
     2161 tcaccgatgt tgctgccgcc gttaacactg gtaaagtacg cagcaatttg ggcgagcaac
     2221 caccagcgag tattcccgct agtgaaagca caaaacaata aacccaaatt acaaagcgaa
     2281 tacccccccc ctaatctggg attccccgct atagactaat tctagcagaa tccgccccta
     2341 tagaccaggc gggaatccat aaaaaaaaca ataatatcaa tatgttaatg ttaaatttaa
     2401 taaaaattta caatttaaac ttctaaaatt tgactcaccg aaaagtgttt tcaaatagcc
     2461 ttcgtagttg ttcgagctga ctgcgatgac ggcgttcgga gagcaccgtc gaatcgcctt
     2521 ctaaaagggc caactcacta cgcactaact cgtacactgc cactgaagtt acgccccgaa
     2581 tctgagggtg gagcgccaag aaatcttcga gtaatctcat ctttaattcg acgggaactc
     2641 gccgcaccga ccacgcttcg aactgccgtt gcacttcgcc gatgtttgct gaattcatat
     2701 tcgcgatctg aacatgaaga tcacttgctt cgacgtaggc ctgagaatgc agatcaatgt
     2761 tcgctttgag ttcaactttt gttacggtcg gctgatgctg aacatttgct agctttgact
     2821 tgcccaccgc gtgagatgca cctcgtgcca agacggcttc gagtcccttt gccaggtcct
     2881 tagaagattt cgccaaaggt gcaatttcat gagcggcgct gga
//
LOCUS       DAOMXO010000272         2921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19263_length_2921_cov_4.309839, whole genome shotgun sequence.
ACCESSION   DAOMXO010000272 DAOMXO010000000
VERSION     DAOMXO010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19263_length_2921_cov_4.309839"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..691)
                     /gene="lepB"
                     /locus_tag="PLH57_06465"
     CDS             complement(35..691)
                     /gene="lepB"
                     /locus_tag="PLH57_06465"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRK02293.1"
                     /translation="MHPDPRKTEAPTNPLAPKPVSLLIKLFKNYFLTIFGTIVFALLV
                     RLYVVEAFRVPTDFMAPTLVAGDHIFAHKMFKTIRRGDVIIFNFPNDPRKDFIKRVIG
                     VGGDTVEIREGVLILNGKEVSIAAPRDGETEVFSEEVGKHRYQVFWNSADSKKMIEVK
                     VPDGHVFVLGDHRSKGQDSRVWGFLSTELVKAKAVFVWLSVAESPGGIRWSRFFKGVE
                     "
     gene            complement(691..2490)
                     /gene="lepA"
                     /locus_tag="PLH57_06470"
     CDS             complement(691..2490)
                     /gene="lepA"
                     /locus_tag="PLH57_06470"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRK02294.1"
                     /translation="MGPTKNIRNFCIIAHIDHGKSTLADRLIERTGTVSQREMQDQYL
                     DNMDIEKERGITIKAQTVRLNYKAKDGQVYTLNLIDTPGHVDFTYEVSRSLAACEGAI
                     LVVDTTQGVEAQTLANVYMALGNNLEIFPVLNKIDLPSADPERVRKDIEDIIGIDASH
                     AVLASAKSKIGIDEILEGAVTYFPPPKNEDEKPLRALIFDSWFDPYQGVVVLARIFDG
                     KVKKGDRIKLYHVGKEFEVQKLGVFSPKFTEVQSLGSGEVGCFICGVKDVRDTKVGDT
                     ICATGIDDLEPLKGFQEVKPMVFSGLYPIDADQYNDLKEAMEKLSLNDSAFTYEPETS
                     SALGFGFRCGFLGLLHMDVVQERLEREYNLNLITTAPSVIFRVTQTNGEVTMVDNPSK
                     LPPITKIQTIEEPFVKLIIHLPNEFVGNVIALCEDRRGIQKKIDYVTADRVMLEYEIP
                     LAEMVFDFFDRLKSITRGYASMDYELLDYREADVVKLDILINGDPVDALSVILHRSKS
                     AYRGRELAKKLKELIERQMFEVNIQAAIGTKILARETLGALRKNVTAKCYGGDISRKR
                     KLLEKQKEGKKRMKQIGTVEIPQEAFLAVLKVD"
     gene            2545..>2921
                     /locus_tag="PLH57_06475"
     CDS             2545..>2921
                     /locus_tag="PLH57_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRK02295.1"
                     /translation="MTEPFKIDQEYDVNIERMSFGPRAVARIHYTEGDRPLVVFVEGA
                     APGDRARIRLTHQHKNYWEAEIVEVLTASNARTQPLCRHFGTCGGCQWQHLTYEAQLA
                     AKKEILLHQIEKTTKVPRSELET"
BASE COUNT          767 a          735 c          634 g          785 t
ORIGIN      
        1 gcgatttcgc cacacccgtt gtgccgccat aggtttactc tactccctta aaaaatcgac
       61 tccatcgaat accgcccggc gactctgcta ccgatagcca aacaaaaaca gctttggctt
      121 ttaccaactc ggtagacaaa aatccccaca ctcgcgagtc ttggcctttg gatcgatgat
      181 cgcccaaaac aaagacgtgt ccgtctggaa cttttacttc aatcattttt tttgaatccg
      241 ccgaattcca aaacacttgg tagcgatgtt tgccgacttc ttctgaaaag acttccgttt
      301 ctccatcgcg aggtgcggca atcgaaactt ccttcccatt gagtatcaag accccttctc
      361 gaatttctac cgtatcaccg ccgacgccta tgactcgctt aatgaaatct ttgcgtggat
      421 catttggaaa attaaaaatt attacgtcgc ctcttcgaat cgtcttaaac attttgtggg
      481 cgaaaatatg atcaccggcg actaaagtgg gtgccataaa atctgtaggc actcgaaacg
      541 cttctacaac gtaaaggcgc acaagcagtg caaagacgat cgttccaaaa atagtcaaaa
      601 aatagttttt aaataattta attaaaagcg aaacgggctt gggagcaagc gggttagttg
      661 gagcttcggt ttttcgaggg tctgggtgca tcagtctacc ttaagcacag ctaaaaacgc
      721 ttcttgcgga atttcaactg tgccgatctg cttcatgcgt ttttttcctt ctttttgctt
      781 ctcgagcagc tttcttttgc gcgatatgtc accgccatag cacttcgcag tcacgttttt
      841 gcgcaacgca cctaaggttt cgcgcgccaa aattttagtt ccgatggctg cctgaatgtt
      901 aacttcaaac atttggcgct cgatcaactc cttaagtttt ttggccaatt cccggcctcg
      961 ataggccgat ttcgaacggt gcaatatcac tgataatgcg tctactggat ctccgttgat
     1021 tagaatatcc aatttgacca cgtcggcctc gcgataatcg agcaattcat agtccatgct
     1081 cgcatacccg cgtgtaatgc tttttaaccg gtcaaaaaaa tcaaacacca tttcagcgag
     1141 cggaatttca tattccagca ttacgcgatc agctgttaca tagtcgattt tcttttgaat
     1201 tcctcgtcga tcctcacaca acgcaattac gtttcctacg aactcgttcg gtaaatgaat
     1261 gatcaactta acgaaaggtt cctcgatcgt ttgaattttg gtgattggcg gcaatttcga
     1321 aggattgtcg accatcgtga cttcaccgtt ggtctgggta actcgaaaaa taacggaagg
     1381 agcagtagtg attagattta gattgtactc gcgctccagg cgttcctgga caacatccat
     1441 gtgcagcaac cccaaaaagc cgcagcgaaa tccaaagccg agtgcagaag aagtttcggg
     1501 ttcgtaggta aatgctgaat cattgagact cagtttttcc atagcttctt tgaggtcatt
     1561 atattgatct gcatcgatcg gatacagacc agaaaaaacc atcggcttta cttcttgaaa
     1621 acccttgagc ggctctagat catctatgcc ggtagcgcag atcgtgtcgc ccactttggt
     1681 gtcacgcaca tcttttacgc cgcaaatgaa acagcccact tcaccactac caagagattg
     1741 aacttcggtg aatttcggcg aaaaaactcc gagcttctga acctcaaatt ccttacccac
     1801 atggtaaagc ttaatacgat cgcccttttt cactttgccg tcaaaaatgc gggccagcac
     1861 taccacaccc tgataagggt cgaaccaact atcaaaaatc aatgcgcgca ggggtttttc
     1921 gtcttcgttt ttgggaggtg gaaaataagt caccgcacct tctaaaattt cgtcgattcc
     1981 aatcttcgat ttcgcgcttg cgagtaccgc gtgagaagcg tcaattccga taatatcttc
     2041 gatgtctttg cgcacgcgtt cggggtcagc cgatggcaga tcgattttat tcaaaaccgg
     2101 aaaaatttcg agattgttcc ctagcgccat ataaacattg gctaaagttt gagcttcgac
     2161 cccttgagtc gtgtcgacca ccaatatggc gccttcgcac gcagcgagcg agcgagaaac
     2221 ttcgtaagta aaatctacgt gtccgggcgt gtcgatcaaa ttaagggtgt aaacctggcc
     2281 gtctttggct ttgtaattga ggcgcacggt ctgagccttg atcgtaatgc cacgttcttt
     2341 ctcgatatcc atgttatcga gatactgatc ttgcatttcc ctctgggaaa cggtgcccgt
     2401 acgctcgatc aatcgatcgg cgagagtaga tttcccgtga tcgatgtgtg cgatgatgca
     2461 aaagtttcgg atattcttag ttggacccat gcgttaaaaa ggagtactgt gctcgagatt
     2521 tagcaccccg cacaggaaaa ctcaatgact gaaccgttta agattgacca ggaatacgac
     2581 gtaaatatcg aacgaatgag ctttggcccc cgagccgttg ctcgcattca ttataccgag
     2641 ggggatagac ccctagtggt gtttgtcgaa ggtgccgccc ccggtgatcg ggcccgaatc
     2701 cggctcaccc accaacataa gaattactgg gaagccgaga tcgttgaagt attaacggct
     2761 tcgaacgccc gcactcagcc actctgtcgt catttcggaa cttgcggcgg atgccagtgg
     2821 caacacctca cttacgaggc tcaactcgcc gctaaaaaag agattttgct gcaccagata
     2881 gaaaaaacca ccaaagtacc ccgctcggaa cttgagacaa t
//
LOCUS       DAOMXO010000273         2916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19308_length_2916_cov_5.460329, whole genome shotgun sequence.
ACCESSION   DAOMXO010000273 DAOMXO010000000
VERSION     DAOMXO010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19308_length_2916_cov_5.460329"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..530
                     /locus_tag="PLH57_06480"
     CDS             30..530
                     /locus_tag="PLH57_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRK02296.1"
                     /translation="MLAAKTQGAVAAVFKAGYKELKKISQIDARNVRTESIANIHRAL
                     GRTALAETHGLEKMVGFLATIGSAAPFVGLFGTVWGIMNSFQGIGATGNANLAVVAPG
                     ISEALIATAAGLAAAIPSVMAYNYFVGRIKSIATEMDTFSQDFLNIVQRSMLKSNSAP
                     TVNAQQ"
     gene            569..1003
                     /gene="tolR"
                     /locus_tag="PLH57_06485"
     CDS             569..1003
                     /gene="tolR"
                     /locus_tag="PLH57_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HRK02297.1"
                     /translation="MGFGVQDISNKTPTTLSEINVTPLVDVMLVLLIVFMVSAPLMQQ
                     GVQVDLPKTASHALDEQQDQIVLVIHRNERISINDNAIEMRDLEKKLAAIYQNKSTRE
                     IYIQADQSVNYGLVAQIMARVRRAGITKVGLVTDPIDSKADR"
     gene            1012..1782
                     /locus_tag="PLH57_06490"
     CDS             1012..1782
                     /locus_tag="PLH57_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HRK02298.1"
                     /translation="MATPPKRNIRRPGSSQDEFGRSLRLSAAVHAILLIGALLSTLVF
                     RREPVMLSPSIRVDLVGLPDIKKKDLNLSEPGPDLSKIEKELEASKPAPAPEPAVEDK
                     PEISLKKKDGKPKKEESRKESLSSAIERIKALAEIENDLKKNKKNTTQVKGNTVSKGN
                     ALTGTSAQDINAYAAGMQARLREHWNLPVWLAKQNLAARVVVFLDDRGYVANTVLAKS
                     SGNTQYDEYVLKTVRMSQPFGPPPSDIQDDGVLLLFPL"
     gene            1827..>2916
                     /locus_tag="PLH57_06495"
     CDS             1827..>2916
                     /locus_tag="PLH57_06495"
                     /inference="COORDINATES: protein motif:HMM:NF015980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02299.1"
                     /translation="MLKLTLISLFLAFTSTWNLVQAGQVYIPVGEARIKKSVLAFSPT
                     KYLGAGFDSGDSKSTARTIRETVLADLTLTNLFTIQNESAFIEKPEAGLTLDAFRISD
                     WSTIGTEFLIKTGISITGGSFAYEVHLYDVLGSKQVLGKRYVARVEEPKLLAHTIAND
                     LYEALTGRPGVFFTKIAMVCDKTGKKEVWTMDFDGSNPKQLTSHRSRAIAPAWSADNR
                     KIAYSVYTKNGRNVENIDLYEMDLKTKAARLLSNRKGINSGAAYSPNGSQIALTMSFL
                     GNPEIFTLNPNTREVTRLTKSFGVDVDPSWSPDSKQIAFISTRSGAPMVYRANVEALK
                     SNPNAATRVTFAGSYNSATAWAPYVGKIA"
BASE COUNT          803 a          736 c          693 g          684 t
ORIGIN      
        1 cggtggtcag gcccagggcc gactgaaaaa ttttagcggc taaaacgcag ggagctgtcg
       61 cggcggtttt caaggctggc tacaaagagt tgaaaaaaat ctcgcaaatc gacgcgcgaa
      121 acgtgcgcac cgaaagtata gccaacattc accgcgcttt gggtcgtacc gcccttgcag
      181 aaacacacgg tctagagaag atggtgggct ttctcgcgac cattgggagt gccgcaccat
      241 ttgtcggact atttggaacg gtttggggta ttatgaattc ctttcaagga ataggcgcta
      301 ctggaaatgc aaaccttgcc gtcgttgcgc caggaatcag cgaagcgctc attgctaccg
      361 ccgccggcct tgctgccgcg attccctcgg tgatggccta taattatttt gttggacgaa
      421 tcaaatcaat tgctactgaa atggatacct tcagccagga ctttctcaac attgtgcagc
      481 ggagcatgtt gaagtcgaat agcgccccta cggtcaacgc acaacagtag caaaaacttc
      541 aaccggtcaa caggccaagg agctcaccat gggtttcggc gttcaggata tttcgaataa
      601 aaccccgact actttgtcgg aaattaacgt cacaccgctg gtcgacgtaa tgttggtgtt
      661 gctgattgtt ttcatggtct ctgcgccgct catgcaacaa ggcgtgcagg tagacttacc
      721 aaaaacagcc tctcacgcgc tcgacgaaca acaagatcag atcgttcttg tgatccatcg
      781 aaacgaacgc atttcgatta acgacaacgc aatcgaaatg cgcgatctcg agaaaaagtt
      841 agcggcaatt taccaaaaca aatctactcg agaaatttac attcaggcgg atcagtcagt
      901 gaactacggc ttggtcgctc aaatcatggc ccgagtgcgc cgggctggta ttactaaagt
      961 agggctcgtc actgatccaa ttgatagcaa ggcggaccga tagagaaagc aatggcgact
     1021 cctccaaaaa gaaacattcg gcgtcccgga agttcgcaag acgaatttgg gcgttctctg
     1081 cggttatcag ctgccgttca cgcaatttta cttattggcg cgcttctttc gacgttggta
     1141 ttccgccgcg aaccggtgat gctctcgcca agcattcggg ttgacttggt cggactgccc
     1201 gatattaaaa agaaagatct taatttaagt gaacctgggc ccgaccttag caagatcgaa
     1261 aaggaactcg aggcttcgaa acctgctccc gcgccggaac ccgcagtcga agataaacct
     1321 gaaatttccc taaagaaaaa agacgggaag ccaaaaaaag aagaatcgcg caaggaatca
     1381 ctctcaagcg ccatagagcg cattaaggct ctggcggaga ttgaaaacga cctcaaaaag
     1441 aataaaaaaa ataccactca agttaaagga aacaccgttt caaaaggcaa cgctctaact
     1501 ggcactagtg cacaagacat caacgcctat gccgcgggca tgcaagctcg tctgcgcgaa
     1561 cattggaatc taccagtgtg gcttgctaaa caaaacttgg cggcgcgcgt agtggttttt
     1621 ttggatgatc gtggttacgt tgcaaacact gtgttggcca aaagttcggg gaacactcag
     1681 tacgacgagt acgtactcaa aactgttcga atgtcccagc cgttcggtcc gccgccgagc
     1741 gacattcaag atgacggcgt tttattgctc tttccgcttt aacgggccaa tgttaacctt
     1801 tttcaaaaga attttaggag actaccgtgc tgaaactgac actcatttca ctattcttgg
     1861 cattcacctc gacttggaat ctggttcaag ccggccaggt ctatattccc gtcggcgaag
     1921 cccgaattaa aaagagcgtt ttagcatttt ccccaaccaa atacctaggg gcgggttttg
     1981 actctggcga ttccaaaagc acagctcgca cgattcgcga aaccgtgttg gcggatctta
     2041 cgttaaccaa cttattcacc attcagaacg aatcggcgtt catcgaaaag cctgaagccg
     2101 gtctcactct cgatgcgttt cgcatctccg attggtcgac gattggaact gagtttttaa
     2161 ttaaaacggg tatttctatc acaggtggga gcttcgccta cgaagtacat ttgtacgacg
     2221 tgctgggttc caaacaagtt ttgggcaaac ggtacgtggc gcgagtcgaa gaacctaaac
     2281 tactagccca cactatcgcc aacgatctct acgaggccct cacgggccgc cccggagtat
     2341 tttttactaa aatcgcaatg gtctgcgaca aaactggaaa aaaagaagtt tggacaatgg
     2401 attttgacgg ctcaaacccc aaacaattaa cttcccatcg ttcgcgcgcc attgccccgg
     2461 cgtggagtgc cgataatcga aaaattgctt attcggttta caccaaaaat ggacgaaacg
     2521 tagaaaacat cgacctctac gagatggacc tgaaaaccaa ggctgcgcgt ttgctgtcga
     2581 atcgaaaggg aatcaattct ggtgcggcat attcccccaa cggatcacag attgctctta
     2641 caatgagttt tcttggtaat cctgaaatct tcacgctcaa tcccaacact cgggaagtca
     2701 ctcgattaac caaatcattc ggagtcgacg ttgacccttc atggtcaccc gatagcaaac
     2761 aaattgcttt tatatcgact cgctccggtg ctcctatggt ttaccgcgcc aacgtcgaag
     2821 cgcttaaatc caatccaaat gctgctactc gcgtaacttt tgcaggcagc tacaattcgg
     2881 caacggcgtg ggcaccgtat gtcggaaaaa ttgcgt
//
LOCUS       DAOMXO010000274         2909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19378_length_2909_cov_4.439033, whole genome shotgun sequence.
ACCESSION   DAOMXO010000274 DAOMXO010000000
VERSION     DAOMXO010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19378_length_2909_cov_4.439033"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLH57_06500"
     CDS             <1..284
                     /locus_tag="PLH57_06500"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRK02300.1"
                     /translation="DEDFPVEGWATDLSISGVGACVEHFLPKDTIVAVELVGETEPKA
                     LTARVAWCNKLPSTGRVIKKKHFKWRMGLEFKFNNEEEKKLISKLVERA"
     gene            284..2011
                     /locus_tag="PLH57_06505"
     CDS             284..2011
                     /locus_tag="PLH57_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRK02301.1"
                     /translation="MRIAFIVGSIAGLAFTLVAMLTESALSNVYVAKLITEAGWLLPL
                     LLAFAILALSAVTLVGTSPKAWEDIFEAFYLDLGRTPPRVWGVRAVTTFLGRAGLSLF
                     DLGGTIIEFGLSLFSKISERFRFHYEDRQIFIAALFAGALSAAFESPFFAALFAIELF
                     ASNNTIVRSSAIVASLVSYGVALLFRGNILSTSPQYSSLKNDLIQTLSAQIQPEASVG
                     AALSLGFHAILTGVLVSLLIFIFVRIQLWASRTFERFFSSGNLLRVGLTGLLISIVYV
                     SAFGGDVDLTRLSDSRIWEDLSWVRLSYASALLLLLFCFCRLALALSGWGSLGIFVPI
                     LTMGAVAGFLVGQILPSDNGGMLIFAGAAVAGTAFFGTPLAMSALLLESSLDVRVWWF
                     GSLAIATSFLFLKHFRIPSFTQLKLASQNLEVYKGRVISLLRGVLVSELLTREVQTLP
                     MNASVQNLREAVLQSPVNIIAVTANDGKYLGLLSLEHVPNSIRAILAGGPESKAAEAL
                     NSILEIRDLVDLSAPIAKPSDEVIVTLKKFSRHPCLAVVDEGGYLCGILFESAVHARY
                     RKLLTKAFT"
     gene            2046..>2909
                     /locus_tag="PLH57_06510"
     CDS             2046..>2909
                     /locus_tag="PLH57_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRK02302.1"
                     /translation="MNLFESLNPQQKQGVLHTEGPVLVIAGAGSGKTKMLTSRIAHLI
                     GEKKAKPHEILAVTFTNKAAGEMRERVAKLLGLMPDAMNPPPVYFGALPVTQPLIGTF
                     HSICTRILRNEMQYTPFRSQFVIYDDSDQISLIKECMQQLQISDKTFSPKSFQFSINS
                     AKCQCLEPQDIAADENSPYMRNLVQLYELYQRELHKNNAVDFGEIITLTVRILHKNPE
                     LLEKYQQRYRYLHVDEYQDTNRAQYLLVRLLASGHRNVCVVGDEDQSIYKWRGADIRN
                     ILDFEKDYPEAT"
BASE COUNT          681 a          662 c          731 g          835 t
ORIGIN      
        1 acgacgaaga ctttccagtt gaagggtggg ctaccgattt aagcatttcg ggagtggggg
       61 cttgtgtaga acactttcta ccgaaagaca cgatcgtggc cgtggagctg gttggtgaaa
      121 ccgaaccaaa ggcactaact gcccgagtgg cttggtgcaa taaacttccg tcgaccggtc
      181 gcgtcattaa aaagaagcat ttcaaatggc gcatggggct ggagtttaag ttcaataacg
      241 aagaagaaaa aaaactcatt tcgaaactcg ttgaaagagc ctgatgcgga ttgcgttcat
      301 cgtcggatcg attgctggcc tagcttttac gctggtcgcc atgttgaccg agtctgccct
      361 ttcgaacgtg tacgtcgcaa aattaattac tgaagccgga tggctgcttc ctcttttgtt
      421 ggccttcgca attttggcgc taagtgccgt cacattggtg gggacttcgc ctaaagcgtg
      481 ggaagatatt ttcgaagcat tttatctaga tcttggtcga actccccctc gggtttgggg
      541 cgtgcgcgca gtcactactt ttttgggtcg ggcgggactt tcgctttttg atctcggcgg
      601 cacaattatt gagtttgggc ttagtttgtt ttcgaaaatt tctgaacggt ttcgctttca
      661 ctacgaagat cgtcaaattt tcatcgcggc cctctttgcg ggggctctct cggcggcttt
      721 cgagagtcct ttttttgccg ctttgttcgc cattgaactc tttgcgtcga acaacacgat
      781 cgtacgatcc tccgctattg ttgcatcact cgtaagttac ggagtggcgc tgctttttcg
      841 tggaaacatc ttgtcgacct ctcctcagta ctcaagcttg aagaatgacc tgattcagac
      901 cttatctgct cagattcagc ccgaagcatc ggttggtgcc gcgttgagtt taggttttca
      961 cgcgattcta acaggtgtgt tggtcagttt gttgattttt atttttgtgc gaattcagct
     1021 ttgggcttct cggaccttcg aacggttttt ttccagcgga aatcttttgc gtgtgggact
     1081 cacgggactt ttaatatcca ttgtttacgt ttcggccttc ggtggagatg tggacttaac
     1141 gcgactgtcc gattcgcgaa tttgggaaga tctatcttgg gtgcgtctct cctatgcttc
     1201 ggcactgcta ctgctacttt tttgtttttg tcgattagcg ttggccttat ctgggtgggg
     1261 ctcgctcggt atattcgtgc caattttaac aatgggagcg gttgcaggct ttttagtggg
     1321 acaaatctta ccttcggaca acggagggat gttaattttc gcaggggcag cagtggctgg
     1381 gacagcgttt ttcggaaccc cgcttgcgat gtcggcgctg ttacttgaaa gtagtctaga
     1441 tgtgcgggtc tggtggtttg gctccttggc gattgcgact tcatttttat tcttgaaaca
     1501 ttttcgaatt ccttctttca ctcagctaaa attagcgagc cagaatttag aagtttataa
     1561 aggtcgcgtc attagtcttc tgcgtggggt gttggtctcg gaattattga cgcgggaagt
     1621 acaaacccta cccatgaacg cgtcggtaca gaacttgcgt gaggcggtgc tccagtcgcc
     1681 ggtgaatatt attgcggtga ctgcgaacga cggtaaatat ttgggattgc tatccctcga
     1741 acatgttccg aacagtattc gagccatact agcgggggga cctgaatcca aagctgcaga
     1801 agcactcaat tcgattcttg aaattcgaga tcttgttgac ctttcagccc caatagcaaa
     1861 gccttctgac gaagtgatcg tgacgctaaa aaaattctct aggcacccgt gtctggcagt
     1921 agtggacgaa ggtggttact tatgcggaat tctcttcgaa tctgccgttc acgcgcggta
     1981 ccgtaaactt ttgactaaag cctttacttg atcgaccaaa aacactgtgt ttatatcggc
     2041 cgatcgtgaa tctattcgaa tcattaaatc ctcaacagaa acagggcgtg ctccacaccg
     2101 aggggccggt attagttatc gcaggtgcgg gcagtggcaa aacgaaaatg cttaccagtc
     2161 gtatcgccca tttgatcgga gaaaaaaaag ctaaacctca cgagattctg gccgtcactt
     2221 tcaccaataa ggcagctggg gaaatgcgag aacgtgtcgc caaattactg ggattgatgc
     2281 cagacgccat gaatcccccg ccggtgtatt ttggggcact tccagtgact cagccactta
     2341 tcggaacctt tcactcgatt tgtaccagaa ttttacgaaa cgaaatgcaa tacaccccgt
     2401 ttcgttcgca gttcgttatt tacgatgact ccgatcaaat ctcgctcata aaagagtgca
     2461 tgcagcagtt gcagatatcg gataaaacct tttctccaaa aagttttcag ttttcgatca
     2521 actctgccaa atgccagtgt ttggaaccgc aagacatcgc tgccgatgaa aatagcccgt
     2581 acatgcgcaa cttggtgcag ttgtacgaac tttaccagag ggagctgcac aaaaataacg
     2641 cagtcgactt cggtgagatc atcactttga cggtgcgtat tctacacaaa aaccctgagt
     2701 tgctcgaaaa atatcaacaa cgctatcggt acctccacgt tgatgagtat caagacacca
     2761 accgggcgca gtatttactg gtgcgtttgt tggccagtgg acaccgcaat gtatgtgttg
     2821 ttggcgacga agaccagtca atctacaaat ggcgtggcgc tgacattcgg aatattttgg
     2881 atttcgaaaa agattacccc gaagcaaca
//
LOCUS       DAOMXO010000275         2908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19395_length_2908_cov_4.172450, whole genome shotgun sequence.
ACCESSION   DAOMXO010000275 DAOMXO010000000
VERSION     DAOMXO010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19395_length_2908_cov_4.172450"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLH57_06515"
     CDS             complement(<1..403)
                     /locus_tag="PLH57_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02303.1"
                     /translation="MAHPANVSQNSPKFKKPSLEQLRTFYKMEIHPYISGLDRKRKSA
                     EAFTLTVSLLIAGFVYFREKNIPRFSNGHDPLIFAGLVGLAIVVVGFAFIALFIRSKS
                     FRSEFKNRVVSRLIERFYPELAYVPMGFVTAD"
     gene            complement(403..972)
                     /locus_tag="PLH57_06520"
     CDS             complement(403..972)
                     /locus_tag="PLH57_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRK02304.1"
                     /translation="MNTNTFLIVAGVLAVVCIFLYNSIIAKRNNVKNAFASIDAMLKK
                     RFDLIPNLVATAQQSAKFEQSTLDMIVRLRSEGTAALGSGSKKAAEIDREMSKALKGF
                     MIQVENYPDLKSNQNFLQLQHALQEIEDQLAATRRTYNATVTIYNNALDMFPSNLFAA
                     MLNFKPEALFEATNDERQNVDVKKLFASK"
     gene            1060..2442
                     /locus_tag="PLH57_06525"
     CDS             1060..2442
                     /locus_tag="PLH57_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02305.1"
                     /translation="MFRSSALFLSVLFWGQLVTSFGFANSPYRENPADDEIEVLKQEV
                     GALQLEVERERIELRNHALLPEIEHLALRIEEITDELDLLREKQKTAKISGYRETLGA
                     RIVSLTTELGRAQHHYRIFSEALEQAEKEIAEKELILLNKRRVLLFFGSPENIRLQKI
                     VEQSEALFQKARKAATVSYQSLKTGKVITSINLNLVFMEIAKLSENAERLLLELRAAR
                     LLAAGDNERLTELLGPEEIKYLNKPIELLQESLVINFLVALRLSADETHFKKRHLSVI
                     EKRRAAGALGWLAHATSGAALLAQVSTTLAVAYALATSGAKAVLGLLKIEGASIKEGS
                     LFDRVQSAIFSPLTAPVSNKLLAIPTESSVSEQVLLDHYFGPGLRSALATNIHRDGGQ
                     QTSDLVDALLTEAGFQNQMWKGFNPPVQFDSRGESQGADCRNILDATRQTVANVLRIM
                     NEGKTTLQKK"
     gene            2603..>2908
                     /locus_tag="PLH57_06530"
     CDS             2603..>2908
                     /locus_tag="PLH57_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02306.1"
                     /translation="MWLMWKRNLQVVAVARYCAFAVGSLFFLSMNIARAEIAYPQFAA
                     PGYVPTPNCAECATLANAPGMPEIKLPFEKNLYETKNGFLYPASATRYLAAPSAPLPT
                     "
BASE COUNT          760 a          642 c          752 g          754 t
ORIGIN      
        1 gatcggcagt cacgaagccc attggcacgt aggcgagttc cgggtaaaaa cgttcgatta
       61 aacgtgatac tacgcggttt ttgaattcgc tgcgaaaaga tttggatcga ataaacagtg
      121 cgataaaggc aaaacccacc actactattg ccaggcctac tagaccggca aatatcagcg
      181 gatcgtggcc gtttgaaaaa cgaggaatgt ttttttcccg aaagtataca aagcctgcga
      241 tcagcagcga aaccgtaagt gtgaacgctt cggccgattt acgtttgcgg tctaggccac
      301 tgatgtacgg atgaatctcc attttgtaaa acgttcggag ttgctcaaga gatggctttt
      361 taaactttgg ggagttctga gaaacgtttg caggatgggc cactacttac tcgcgaaaag
      421 tttttttacg tctacgtttt gacgttcatc gttcgtagcc tcgaagagcg cttcgggttt
      481 gaagttaagc atggctgcaa aaagattcga aggaaacatg tcgagcgcat tgttgtaaat
      541 ggtaaccgta gcgttatagg tccgcctagt agccgcgagt tggtcttcga tttcttgtag
      601 cgcgtgttgg agctggagaa agttttgatt cgattttaga tcagggtagt tttccacctg
      661 gatcatgaac cccttgagcg ccttgctcat ttcgcggtcg atttcggcgg cttttttgga
      721 accgctaccg agcgctgctg tcccttcgct tcgcaggcga acaatcatgt ccagtgtcga
      781 ttgttcaaac ttggccgact gctgggcggt ggccaccaag tttggaatca aatcaaatct
      841 ttttttgagc atggcatcga tcgaagcgaa cgcgtttttc acgttgttgc gctttgcgat
      901 gatcgagttg tagagaaaaa tgcagacgac agccagtacg ccagcaacga tcagaaaggt
      961 gttggtgttc attgctctaa gggtaccagg aaattcagtt gcgtaaatac ggttcgtaat
     1021 aggattgatt tggtcgatta aatatcgtag taggatggcg tgtttaggag cagcgcatta
     1081 tttttaagtg ttttattttg gggccaattg gtcactagtt ttggatttgc taattctcct
     1141 tatcgcgaaa atccggcgga cgacgaaatt gaggttttaa aacaagaagt tggagccctg
     1201 cagctcgaag ttgagcgtga aagaatcgag ttgcggaatc atgcgttgct gccggagatt
     1261 gaacacctcg ccctcagaat agaagaaatc actgacgaac tcgatttgct tcgcgagaaa
     1321 caaaaaactg caaaaatttc aggttatcgc gaaactttag gagcacgaat tgtatctttg
     1381 accacagagt tagggcgcgc ccaacaccat taccgtattt tctctgaagc gttagagcag
     1441 gccgaaaaag aaattgcgga aaaagagctg atacttttga acaagcggcg agttttactc
     1501 ttctttggtt cgcccgaaaa cattcggttg cagaagattg tagaacaaag tgaggcgctt
     1561 tttcagaagg cccgtaaggc agccacggta agttatcaga gtttaaaaac cggaaaagta
     1621 ataacgagta tcaatttaaa tttggtgttt atggaaattg ccaaattatc agaaaatgcc
     1681 gaacggctac tcttggaact gcgagcggca cgtttgcttg ctgccggtga caacgaacgt
     1741 ttgaccgaat tgttggggcc cgaggaaata aagtatctga acaagcctat agagttgttg
     1801 caggaaagtt tagtgatcaa tttcttagtg gcgttgcgac taagtgcaga cgaaacccat
     1861 tttaaaaaac gtcatctttc agtaatcgag aaaaggcgag cggcaggagc cctgggctgg
     1921 ttggcccatg cgacgagcgg ggcagcgtta ttagcccagg tgagcaccac cctcgcagtc
     1981 gcttacgcat tggcgactag tggcgcaaag gcggtgcttg gactgctaaa aatagaaggt
     2041 gcttcgatca aagaaggtag tcttttcgat cgagttcaaa gcgctatttt ctccccgttg
     2101 acggcgccag tttcgaacaa actcttggcg attcccactg aaagttcggt ttcagaacaa
     2161 gtgcttctgg accactattt tggacccggt ttgcgttcag ccctggctac gaacattcac
     2221 cgagacggtg ggcagcagac ctctgatctg gtcgatgcat tgttaacgga agcgggcttt
     2281 cagaaccaaa tgtggaaagg cttcaatcct ccggttcaat ttgattcgag gggagagtcg
     2341 cagggagctg attgtcgcaa tattttagat gccacgcgcc agacggttgc gaatgttctt
     2401 cgaataatga acgagggcaa aacaacactc caaaaaaaat agagcccgag cgagagtctg
     2461 gtactgtcta aacttttgac acctgcctcc gcagctacaa acttttttgg tcgctgctcc
     2521 ggcagaacaa gtcgcaaaaa ctggcccgcc ccttgctcta catctcagtc gtcagacatc
     2581 gaagaagaga gaagagtggt ttatgtggtt gatgtggaag cggaatcttc aagtagtagc
     2641 agtagctcga tattgtgcgt ttgcagttgg atcgctcttt ttcctttcga tgaatatcgc
     2701 acgcgccgag attgcgtacc cgcaattcgc ggccccaggt tatgttccca ctccgaactg
     2761 cgctgaatgc gcgaccctgg ccaatgcccc tgggatgccg gaaattaagc tcccattcga
     2821 aaaaaaccta tacgaaacca aaaacggttt tttgtaccct gcatcggcga cccgctattt
     2881 ggcggcaccc tcggcgcctc tccccacc
//
LOCUS       DAOMXO010000276         2899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19491_length_2899_cov_4.131857, whole genome shotgun sequence.
ACCESSION   DAOMXO010000276 DAOMXO010000000
VERSION     DAOMXO010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19491_length_2899_cov_4.131857"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="era"
                     /locus_tag="PLH57_06535"
     CDS             complement(<1..972)
                     /gene="era"
                     /locus_tag="PLH57_06535"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRK02307.1"
                     /translation="MNTTNQTQPTSFRCGFVTLLGLPNDGKSTLFNKLTDSNLAIVTP
                     KAQTTRTLLRGHVASSTCEFVVVDTPGLQEGTKALNQAIGRNAVRAAANAMAGGELML
                     LVIDAPSLGAQLRDHKSHTPPMLLALERIVTASFENVDLPLKVRVIPVFNKADLVRKP
                     EHRTEIENTVTTFATRLFSDLQSGLWLSSHTESGISELLETIEANLPAGNPGELFESD
                     TLTDQATRTIAAEYIREQCFLQLGDELPYSTAVEIETFDESDPALTRIQATIFVEKES
                     QKGMVIGSGAKKIKAIGTKSRERIEALIERKAFLGLKVKVSANWAGKS"
     gene            complement(899..1954)
                     /locus_tag="PLH57_06540"
     CDS             complement(899..1954)
                     /locus_tag="PLH57_06540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRK02308.1"
                     /translation="MIISVFIPHGGCPVRCSFCDQKTSGGVAVAPAAITKHIAAHLAT
                     AEKQTLGSPLPEVAFYGATFTALSTTLQTTYLDAVLPFIEKGEVGGVRISTRPDAIDS
                     AWLLFLKQRYRLSTVEIGAQSFNPIVLQRMGRGHSVTDVEEAIKKLKDISLTIGLHLL
                     VGCPSEGPTDDQLNLDGVLKLRPDFVRIHPLLVFKKTALADDVMNQNFVPLDLESAIE
                     RTARLVAALEAAHIPVFRIGLQPNEVMKDELMAGPYHPAFGELVRSRIWRHRLADQLK
                     QERIREALEITLEIPPRLVSQVRGQKNCNIQWLNNNFQLTLGGQYMIKTLGHEYHQSN
                     STDVLQMRIRYPAGPSE"
     gene            complement(1951..2682)
                     /gene="rnc"
                     /locus_tag="PLH57_06545"
     CDS             complement(1951..2682)
                     /gene="rnc"
                     /locus_tag="PLH57_06545"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRK02309.1"
                     /translation="MQSIEQLLQHHFRNPKLLEVALIHKSFGNENRAELATSDRDNEQ
                     MEFLGDAVLDLAISELLLELFPQLPEGELSKLRASLVNEHTLAQVARSLQLGDHVKLG
                     RGEDLTKGREKDSILSGTLEAVLAAIYRDAGYDVAKTWAHERFRAHAAQAGADERDGL
                     HLQDHKTRFQEVAQALYKTAPRYTVTSATGPDHEKTFVIEVSIHNILLAEGTGRSKKE
                     AEQQAAKNAFDRFGPKLGHPDLKKK"
BASE COUNT          652 a          702 c          778 g          767 t
ORIGIN      
        1 actttttcca gcccagttgg ccgatacttt aactttaagt cctaaaaagg ctttacgttc
       61 aatcagcgct tcaatacgtt cccgcgattt tgtgccgatg gctttgattt tttttgctcc
      121 cgaaccgatg accataccct tttgactttc tttttctaca aaaatggttg cctgaattcg
      181 agtgagggct gggtcggatt cgtcgaaagt ctcaatctcc actgcggtgg aatacggcag
      241 ttcgtcgccc aattgcaaga agcactgttc gcgaatgtat tcggctgcaa tcgtgcgagt
      301 ggcctgatcc gtgagtgtat cgctctcgaa gagttcgcca gggtttccgg ccggtaaatt
      361 ggcttcgatc gtttcaagta actctgaaat tccgctctcc gtatgggacg acagccagag
      421 tcccgattgg agatctgaaa acaaacgtgt tgcgaaggtg gtcaccgtat tttcgatttc
      481 ggtgcgatgt tctggcttcc gcacaaggtc agctttgttg aacactggaa tgacccgaac
      541 tttaagtggg aggtcaacgt tttcaaaaga cgcagtaacg atgcgctcga gcgccagcaa
      601 cattggcggc gtgtgagatt tgtggtctct gagttgggct cccaaactgg gagcatcgat
      661 cacaagcaac atgagctcgc caccggccat cgcattagcc gctgcgcgca cggcgttccg
      721 gccgatcgct tggtttaagg ccttggttcc ttcttgcaag cctggcgtgt cgacaacaac
      781 aaactcgcaa gttgaactgg cgacgtggcc ccgcagaagc gtgcgggtgg tttgggcctt
      841 gggtgttacg atggcaagat tggaatctgt cagcttatta aaaagcgtgg atttgccgtc
      901 attcggaagg cccagcaggg taacgaatcc gcatctgaag gacgtcggtt gagtttgatt
      961 ggtggtattc atggccaagc gtttttatca tgtattgacc gccaagtgtt agctgaaagt
     1021 tattatttaa ccattgaata ttacagtttt tttgaccgcg tacctggctt accaggcgcg
     1081 gggggatttc cagtgtaatc tccagggctt cgcgaatgcg ttcttgtttt agctggtcgg
     1141 ccagtcggtg ccgccaaatg cgcgatcgca cgagttctcc gaaagcaggg tggtaggggc
     1201 cggccattaa ctcgtctttc atgacttcgt tgggttgaag cccgatacgg aagaccggaa
     1261 tatgagctgc ctcaagggca gcaacgagcc tggcggtgcg ctcgattgca gattcaagat
     1321 cgagcggtac gaaattctgg ttcatcacgt catcggccag cgcggttttc ttaaacacca
     1381 gaagcgggtg aatacgtacg aaatcgggcc gtagttttaa tacgccgtcg aggtttaatt
     1441 gatcgtccgt agggccttct gatgggcatc ctaccaatag gtgcagtccg atcgttaatg
     1501 agatgtcttt aagtttttta atagcctctt ccacatcagt cactgaatgg ccacgcccca
     1561 ttcgttggag tacgatggga ttaaaagact gcgcaccaat ttcaaccgta ctcaaccggt
     1621 agcgctgttt caaaaacaac agccaggcgg agtctatagc gtccgggcga gtactaatgc
     1681 ggactccccc cacttcgccc ttctcgatga acggcagcac cgcatcgagg taagtagtct
     1741 gcaaggtcgt ggatagggcc gtaaaagtag cgccatagaa agcgacctcg ggcagcggcg
     1801 aaccgagcgt ttgtttttcg gcagtcgcga gatgggctgc gatgtgtttg gtaatggcgg
     1861 cgggtgctac agccaccccc ccgctcgttt tttgatcgca aaagctgcag cgcacggggc
     1921 aaccaccgtg tggaataaat actgaaataa tcatttcttt ttcaaatctg gatgccccag
     1981 tttgggccca aaacggtcga acgcattttt tgctgcctgt tgttcagctt cttttttgct
     2041 tcgcccagta ccttcggcca gtaatatatt gtgaatcgac acttcgatta cgaaggtttt
     2101 ttcatggtca ggtccagtgg cggaagtgac ggtgtaacgc ggcgctgttt tatagagcgc
     2161 ttgggcaact tcttgaaagc gggttttatg atcttgaaga tgtagcccat ctcgttcgtc
     2221 agcaccggct tgagcggcgt gtgcccgaaa gcgttcgtga gcccaagttt tcgctacatc
     2281 gtacccggcg tcacggtaaa tagcggctag aaccgcctct agggtgcccg acaaaatact
     2341 atccttttcc cgtcccttcg tgaggtcttc cccacgtccc agttttacat ggtcaccaag
     2401 ttggagagaa cgcgccacct gtgcaagcgt gtgttcgttg acgaggcttg ctcgtagttt
     2461 agagagctca ccctctggca gttgcggaaa gagttcaagc aggagttcgg agatcgctaa
     2521 gtcgagaacc gcgtcgccca aaaattccat ttgttcattg tcgcggtctg aggtggcaag
     2581 ctcggcacgg ttttcgttac caaaggactt gtgaatgagc gcgacttcaa gaagtttagg
     2641 gttccgaaag tgatgttgca gtagttgttc gatgctctgc attgttttca ttggacaaaa
     2701 tgaaaccact ttttcaacta aaaatcatca aaattgccac gggtccaccg actaattcta
     2761 tcatgaaacc gtttaaaggt gacttgccat tctccgaaaa agcccgctag cattaggggc
     2821 gagtggatca tttttagacg ttgaaccaaa ttccgcgaaa gcggggattt ttctcaacgg
     2881 gggcaaaact gtgcgttcg
//
LOCUS       DAOMXO010000277         2896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19523_length_2896_cov_5.251320, whole genome shotgun sequence.
ACCESSION   DAOMXO010000277 DAOMXO010000000
VERSION     DAOMXO010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19523_length_2896_cov_5.251320"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLH57_06550"
     CDS             <1..377
                     /locus_tag="PLH57_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02310.1"
                     /translation="PGKAFDVQVTEATKVEGGRYRVKALVDGTQRVGLYDPETETFDE
                     GLYTLSYERGKKIAEMDEEVRYLKDRVEKLEKENYQLKLRLKYVHAGKGDPNKVVDPN
                     TDAGSDDDDVKPSSKKDEKTGA"
     gene            384..1337
                     /locus_tag="PLH57_06555"
     CDS             384..1337
                     /locus_tag="PLH57_06555"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02311.1"
                     /translation="MIEAAIDIGTNTMLLLVAEVDGATAAGTRVPRVLHRVLAEEIQY
                     PRLGQGVHANRNFSPDAMTRAREVFQKYKKICDELGVKKISAMATSASRDSLNAKEFY
                     DSIRGDIGIDVQIISGESEAKLTFLGGLLPMNNPKESAVLDIGGGSTEFVLLDSKTGG
                     LNSQSVNIGCVRATELFLKGDPYERASLETLETNLRQFWKTIKPDLSEELKKREWVGV
                     AGTPTTLAAIQLGLSSFVSEKVDGYRLTRCAIGDWYESLALKTTAQRASNPLMSTGRA
                     DIMVAGTAILLTAMETFEREDIMVSCRGLRHGVLMYPGLVG"
     gene            complement(1341..1529)
                     /locus_tag="PLH57_06560"
     CDS             complement(1341..1529)
                     /locus_tag="PLH57_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02312.1"
                     /translation="MNSKKLEPGTQNQNEEMIRVNGKGQVIDMLRSADAQFRETILRG
                     IEKRDPKLARELRRELAS"
     gene            complement(1548..1961)
                     /locus_tag="PLH57_06565"
     CDS             complement(1548..1961)
                     /locus_tag="PLH57_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02313.1"
                     /translation="MSDSIRTDNLRFEQHKLQLESQQEKQLKDLREKQRIEVEKLTEH
                     YNNTKKDLERAYQIELSSDRDEYERRLSETRKSHAKTIVEEKLNGEDEVEKVRARYQE
                     QLAKYRENAERSVEEVRRQTDEAVNNIKARAKMRG"
     gene            2064..2405
                     /locus_tag="PLH57_06570"
     CDS             2064..2405
                     /locus_tag="PLH57_06570"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRK02314.1"
                     /translation="MNDLMQTVESKTPLLKRIFRKEPSSVGLSWTNGEESEIQFSRLR
                     FECLCAECVDEWTRERRVQSDQIAPDIRPLAIDPVGRYAVQIRWSDGHSAGIYTYETL
                     YRLSTSKNGEK"
     gene            2410..>2896
                     /locus_tag="PLH57_06575"
     CDS             2410..>2896
                     /locus_tag="PLH57_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRK02315.1"
                     /translation="MSTQNLVFLGAKRTPFGAFGGSLRDASPADMATAASKAALVQSG
                     VAPSEIQHVIVGNVVQATADAILVPRHTGLKSGVPLEIPALGVNRLCGSGFQVVVDAY
                     AQMCAGDTEVALVGGTESMSLVPYVVRGARWGSKMGHTQFEDYLTAALTDTYCGCSGP
                     PC"
BASE COUNT          696 a          676 c          763 g          761 t
ORIGIN      
        1 atcctggaaa agcatttgac gtgcaggtca cagaggccac taaagtcgaa ggcggtcgct
       61 atcgcgttaa ggcgttggtc gatggcactc agcgtgttgg gctatacgat cccgagacag
      121 aaacctttga tgagggtctg tatacgcttt cttacgagcg aggaaaaaag attgctgaga
      181 tggacgaaga agttcgttac ctcaaagacc gtgtcgaaaa gctggaaaaa gaaaattatc
      241 agctaaaact gcggttaaaa tacgttcacg cgggtaaggg cgatcccaac aaagtagtgg
      301 atcccaatac cgatgcgggc agtgacgacg acgacgtaaa gcccagctct aaaaaagacg
      361 aaaaaacagg cgcataaaaa tttatgatag aggcagcaat cgatatcggt accaacacga
      421 tgctcttgtt ggtcgccgag gtagatggtg ccaccgcggc cggaactagg gtacctcgag
      481 ttctgcaccg ggttctcgct gaggagattc agtatccaag actaggtcag ggtgttcacg
      541 ccaaccgcaa cttttcgcct gacgcaatga cacgcgcacg tgaagttttt caaaagtata
      601 aaaaaatatg tgacgaactt ggagtgaaaa agatctccgc gatggcgacc agtgcttcgc
      661 gcgattccct taatgccaaa gagttctatg attccattcg aggtgacatt ggaatcgatg
      721 tgcaaattat ttcaggagag tcagaagcca aactcacttt tctaggtggg cttctaccga
      781 tgaataaccc gaaagaatcg gccgtgctcg atatcggtgg gggtagcact gaatttgtgc
      841 tgctcgattc aaaaactggg ggtttaaaca gtcaaagtgt taatatcggc tgtgtgcgag
      901 ccacggagtt gtttcttaag ggcgacccct acgaaagagc ttctttagaa acgctcgaaa
      961 ccaatttgcg ccaattttgg aaaaccatta agccggatct aagcgaggaa ctaaagaaga
     1021 gagagtgggt aggggtggcc ggaacgccaa caacgctcgc tgctattcaa ttgggacttt
     1081 ccagttttgt ttctgaaaaa gtagacgggt atcgtctcac tcgctgtgcg attggcgatt
     1141 ggtatgagag tcttgcactg aaaactaccg ctcagagagc gagtaatccg ttgatgagca
     1201 ctgggcgcgc cgacattatg gttgcaggta ctgcgatatt actgactgcc atggaaacgt
     1261 tcgaacgcga agatattatg gtaagttgca gagggcttcg ccacggggtg ttaatgtacc
     1321 ccggcctagt aggttaaata ctagcttgcg agttcgcggc gtagttcgcg tgcaagttta
     1381 gggtctcgct tttcaatgcc acgcagaatt gtttcccgaa attgggcgtc agcgctacga
     1441 agcatgtcga tcacttggcc tttgccattc acgcgaatca tttcttcgtt ctggttttgg
     1501 gttccgggtt ctagcttttt ggagttcatt cgcgtcctcg cttatagtta cccacgcatt
     1561 tttgcgcgag ctttgatatt atttacagcc tcgtctgttt gccgacgaac ttcttctact
     1621 gaacgttctg cattttcgcg gtacttggcg agctgttctt ggtagcgagc gcgaaccttt
     1681 tctacctcgt cttcgccgtt caatttttct tccacgatgg tttttgcgtg acttttacgg
     1741 gtttcggata gccgtcgttc gtattcgtcg cgatcacttg aaagttcgat ttgataggct
     1801 ctttcgaggt ctttcttggt gttgttatag tgttcagtca gtttttcgac ttcgatgcgt
     1861 tgtttctctc gaaggtcctt cagctgcttt tcttgctgcg attcgagttg cagtttatgc
     1921 tgctcaaatc ttaggttatc cgttcgaata ctgtctgaca tttcctacgc tcctaagcac
     1981 cattttaaga tgttcgcccc tcgattgcga agacggacga ttgtgctccg gggcaaccat
     2041 tgcctatctc ctgggagtgt gtgatgaatg accttatgca aactgtggag tccaaaacac
     2101 ccctgctaaa gcgaattttt cgcaaagaac cgtcgagcgt tggcctttcg tggaccaatg
     2161 gcgaggaatc ggaaattcaa ttttccagac tgcgtttcga gtgtttgtgc gccgaatgtg
     2221 tcgacgaatg gacgcgcgag cggcgcgtac aatccgatca aattgctccc gacattcgcc
     2281 ccctagccat cgaccccgtg gggcgatacg cggtgcagat acgttggtcc gacggacaca
     2341 gcgcaggcat ttacacctac gaaacattgt atcgactttc gacatcaaaa aacggagaga
     2401 agtaacacca tgagcactca aaatctcgta tttttaggag caaaaagaac cccatttgga
     2461 gcattcggcg gaagcctgcg tgacgcgtcg ccagcggata tggcgaccgc tgcgtctaag
     2521 gcggcgttgg tgcagtccgg tgttgcaccg agcgaaattc aacatgtaat cgtcggcaat
     2581 gtagttcaag cgacagctga tgccatattg gttccccgtc acactggatt aaaaagtgga
     2641 gttccactcg aaatacccgc gctcggtgtc aaccgccttt gcgggtccgg ctttcaagta
     2701 gtcgtcgacg cctatgcaca aatgtgcgcc ggtgatactg aggttgctct agttggcggt
     2761 accgaaagca tgagtttagt tccgtacgta gtgcgtggcg cgcggtgggg cagcaaaatg
     2821 ggccacacac aattcgaaga ttatcttacc gccgctctta cggacaccta ttgtggttgc
     2881 tctggtcctc cttgtt
//
LOCUS       DAOMXO010000278         2893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19558_length_2893_cov_5.195208, whole genome shotgun sequence.
ACCESSION   DAOMXO010000278 DAOMXO010000000
VERSION     DAOMXO010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19558_length_2893_cov_5.195208"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLH57_06580"
     CDS             <1..354
                     /locus_tag="PLH57_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02316.1"
                     /translation="WLVLLGLGVFLGAPSVQAGANDRIESSISRGRMAQGWEAGNRDG
                     RAVGRILANPKASKVDRWSAMIRIGTVNHYRSQYMKYAIGFDEGYQAGLSEAPVARQS
                     PRTPTNGLTVEIRGK"
     gene            complement(361..1530)
                     /locus_tag="PLH57_06585"
     CDS             complement(361..1530)
                     /locus_tag="PLH57_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HRK02317.1"
                     /translation="MKDWRDSYSSVEDLAQAGFVTPSEFTALKNLERRYQIRVPHYYA
                     SLFTKSENCPIKLQALPSVAEQAPSLPDWLRDLSATAYGRLEPWTADSIGDVKNLAAP
                     RLTHRYPNRALLHTTTLCALTCRFCFRKSHLNEKESALYEGSFDAAFEYLNKHPEVTE
                     LVLTGGDPLSLTDLALERLLDRINGVAHLRTIRLHSRAPVTSPDRIQPELLDVLKRFV
                     HNSSKTIQLVTHFNHPLELSPLALERLRLLRLSGISLFNQTVLMRRVNNEPSTLSKLY
                     QTLYENGVGALYLHHPDWTPGTFHFRVTIPQGQAITRALRGQLSGPAMPDYVLDSPEG
                     DGKIPLTDSHWLRPVSERITRDGFTAQLFERPRKTASSHESHTRAQFYLDAANVD"
     gene            complement(1595..2743)
                     /locus_tag="PLH57_06590"
     CDS             complement(1595..2743)
                     /locus_tag="PLH57_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HRK02318.1"
                     /translation="MAVQIITVNKVRERFQPAGKGVWSSVPDSDWHSWIWQQQQRIKS
                     MEQLEKAINVTPDERLAFEKSNEMFHMGITPYYATLMDKDDPNCPVRQQSVPKTSELT
                     ILATDLEDPLAEERDMPVPGVTHRYPDRVLFYTTHNCPVYCRHCTRKRKVSDPGSAAQ
                     LAQLENGLKYIEGNKAIRDVIISGGDPLSLGDDRLEWIISRLRACDHIEIIRLGSRNL
                     VTLPFRITDAFINMLRKYHPIHINTHFNHPKECTREAFDTAAKLADAGFTLGNQAVLL
                     KGVNDDPAIMKELMHRLMLMRIRPYYIYQCDLSQGISHFRTPIEKGIEIIEALRGHTS
                     GLCVPHFVVDAPGGGGKIPIMPNYVVKHEGKQWTLRNYAKKQYTYYEP"
BASE COUNT          680 a          660 c          849 g          704 t
ORIGIN      
        1 tggctcgtgc ttttagggct tggagtgttt ctaggcgcgc ctagcgttca agcgggtgca
       61 aatgatcgta ttgagagttc gatttcgcgc ggtcgcatgg cgcagggctg ggaagctgga
      121 aaccgcgacg gtcgtgcagt gggtcgcatt ttagcaaatc caaaagctag caaagtcgat
      181 cgttggtcgg cgatgattcg catcggaacg gtcaatcact atcgcagcca atacatgaaa
      241 tacgcgattg gattcgacga aggctatcag gccggattga gcgaagctcc ggtagcacgc
      301 caatctccac gtacgccgac caatgggctc accgtagaaa ttcgcggaaa ataagaagtc
      361 ttagtcgaca ttcgccgcgt cgaggtaaaa ctgcgcacga gtgtgtgact cgtgagaaga
      421 cgccgtcttg cgtggacgct cgaatagttg cgcggtgaat ccatcgcggg tgattcgttc
      481 acttaccggc cgcaaccaat gtgaatcggt gagaggaatt tttccatctc cttctggaga
      541 gtcgagcacg taatcaggca tagccgggcc cgaaagttgg ccacgaaggg cgcgcgtgat
      601 ggcttggccc tggggaattg tcacgcgaaa atgaaacgtt ccaggtgtcc agtccgggtg
      661 atgcaaataa agcgcgccaa cgccgttttc gtaaagggtc tgatacaact ttgaaagagt
      721 tgaaggctcg ttgttcactc gccgcatgag cactgtttga ttgaagagtg atatgccaga
      781 gagtctaagc aaacgaaggc gttcgagcgc caaggggctc agttcgagtg ggtgattaaa
      841 atgagttacc agttgaatgg ttttagaact gttgtggacg aaccgcttaa gtacgtcgag
      901 aagttcaggt tgaatgcgat cgggactcgt tacgggcgca cgggagtgaa ggcgaatcgt
      961 cctaagatga gcaactccgt tgattcggtc caataaacgc tccagggcga ggtcggtaag
     1021 cgagagcggg tcgccaccgg ttagtacgag ttcggtgacc tcggggtgtt tgttcaaata
     1081 ctcgaatgca gcgtcgaacg aaccctcgta aagcgcagac tctttttcgt tgagatggga
     1141 ttttcgaaag caaaaacggc aggtcaacgc acaaagtgtg gtggtgtgga gtagcgcgcg
     1201 attcgggtag cggtgagtaa gtcgcggggc cgccagattt ttaacatctc caatgctgtc
     1261 ggccgtccac ggctccaggc gaccgtaggc cgtggcgctg aggtctcgaa gccagtcggg
     1321 caggctcggg gcttgttccg cgacggaggg caacgcctgg agtttaatgg ggcagttttc
     1381 ggatttggta aacaacgacg cataatagtg aggtactcga atttggtaac ggcgctcaag
     1441 attcttgagg gcggtgaatt cagaaggcgt gacaaagcct gcctgggcta ggtcttcaac
     1501 gctcgagtag gagtctctcc agtctttcat gtgccgacat ggggtaacac aactctctat
     1561 ctgcggacaa gagaacgaac attcagtgca ggggtcacgg ttcgtagtag gtgtattgtt
     1621 tttttgcgta gttgcgaagg gtccattgtt tgccttcgtg tttgaccacg taatttggca
     1681 tgatcggaat tttaccgccg ccaccgggcg catcgacgac aaagtgcggt acgcaaagcc
     1741 ccgacgtatg cccacgcagg gcttcaatga tttcgatgcc tttttcgatt ggcgtacgaa
     1801 aatgagaaat cccttgagaa agatcacact gataaatgta gtacgggcga atgcgcatga
     1861 gcatgagccg atgcatgagc tccttcatga tagccggatc gtcgttaacg cccttcaaaa
     1921 gcaccgcttg gtttcctagg gtaaaaccgg cgtcagcaag tttggccgca gtatcgaacg
     1981 cctcgcgcgt gcattccttc ggatggttga agtgagtgtt aatgtggatc gggtgatatt
     2041 tacgaagcat attgatgaat gcatcggtaa tccgaaacgg aagagtcact aaatttcttg
     2101 atcccagccg aatgatctcg atgtgatcgc aagcgcgtag gcgagaaatg atccactcca
     2161 aacggtcgtc gcccagcgaa agtggatcgc cgcccgagat aataacgtcg cgaatcgcct
     2221 tgttgccttc aatgtacttt agcccgtttt caagctgagc tagttgagcc gcacttcctg
     2281 gatcgctcac cttgcgtttc cgcgtgcagt gtcggcaata cacagggcaa ttgtgagtgg
     2341 tataaaacaa aacacggtca ggatagcggt gtgtgacgcc aggtactggc atgtcgcgtt
     2401 cttcggccag tgggtcttct aggtcggtgg ctaaaatcgt aagttcgctg gtttttggga
     2461 ccgattgctg gcgtacgggg cagttggggt cgtctttgtc catgagcgtg gcgtaatagg
     2521 gcgtgattcc catgtggaac atttcattac ttttttcgaa agccaggcgt tcgtccggtg
     2581 tgacgttgat cgctttttcc agctgttcca tcgacttaat tcgctgttgt tgttgccaga
     2641 tccaggaatg ccaatcggaa tctggaaccg agctccagac gcccttgcca gccggttgga
     2701 acctttcgcg aaccttattg accgtgatga tttgaactgc catgtgagac ttcctccata
     2761 aaaaacgccg ctgttttaag ggtttagggg gtttttaaga atcgcgcaag tggaacccgt
     2821 tggagaaaat gaaaaaaaat gcggataatg caacattttt actcaagttt gctaaaatat
     2881 tcccgatagg gcc
//
LOCUS       DAOMXO010000279         2892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19568_length_2892_cov_3.652802, whole genome shotgun sequence.
ACCESSION   DAOMXO010000279 DAOMXO010000000
VERSION     DAOMXO010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19568_length_2892_cov_3.652802"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..251)
                     /locus_tag="PLH57_06595"
     CDS             complement(51..251)
                     /locus_tag="PLH57_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02319.1"
                     /translation="MNLLSQYIKDKEYDVRMTERGLSKGTVLREDVEKNQKKLADDSE
                     NLATIDVSVLYDQVRQEKSPLT"
     gene            complement(303..1142)
                     /locus_tag="PLH57_06600"
     CDS             complement(303..1142)
                     /locus_tag="PLH57_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020329119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HRK02320.1"
                     /translation="MRVLKVDYRSPNAAEEFARSLKETGFAVLNHHPISHQLIEDSFS
                     EWARFFESPSKHELLYDKKLQAGYFPFRAETAKDYKVSDLKEFFHYYPKRGDLPGLAQ
                     RATPELYKQLHGLGAELLGWVEDHTPPETKGRFSMPLRKMIDESDDTLLRPIHYPPLS
                     GAEESGAIRAAAHEDINLITLLPAATAPGLQVKDLDGNWHEVECDPCDIAVNSGDMLQ
                     MASGGYYVSTTHRVVNPTGALAKTSRYSMPLFLHPAGNVRLSPSHTARSYLNERLGQL
                     GLL"
     gene            1246..2715
                     /locus_tag="PLH57_06605"
     CDS             1246..2715
                     /locus_tag="PLH57_06605"
                     /inference="COORDINATES: protein motif:HMM:NF021424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRK02321.1"
                     /translation="MMYRSKALLTRILAVQALLLGVSTSQTQANPQFQRINLEHLYVG
                     VEYTFQNRMLLNDPAESPHDVSPKKYRLMLRFFRAYQQILSDLGFGPVSHSIGFLGSP
                     RGVFYRDPRTPNDELFRTLGTAYDSILHSDMRVVEVAEKPKKLTQLESTANLIYAAAS
                     KVNLKPYLLPGGMWSGGAHFNIGSARLEESPFIRFPNLLPNLLAYFDQHASLLYGFGE
                     AFDVGNEGTAVPYHGYGEVSQFRRILKDFSVRYEEFRKIKRPGLHQRVALLASFLRDL
                     KILNRHESFINVERLLSSFSFEDPRNPTIANSGDRNSIEIRTLRMPPTAAHVAELGWF
                     FARLLEKLSEPGLLVQPVAPVNFDIMLTATAIEADWQLVKKDLNLQSPLLDEMIDEQT
                     AMLQTKTFRTLDIPTAEIFSAFSPLAEKGRAFEMRVPSYYSKKMSINGRELTTQTLQL
                     GEQTFEVAYLHSDAFDISPEKIRKNRLRINCQNILLPND"
     gene            complement(2712..>2892)
                     /locus_tag="PLH57_06610"
     CDS             complement(2712..>2892)
                     /locus_tag="PLH57_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02322.1"
                     /translation="YLFASAYFRMRGRAKATEGGGKRRKKKSHLRLVVNNEVLEEFED
                     DEEDDDEEPGKPTFH"
BASE COUNT          725 a          743 c          658 g          766 t
ORIGIN      
        1 cgtggatttt ccacaacagc tctttgatca aaaatcgtcg tttttaattt ttaagtcaga
       61 ggcgactttt cttggcgaac ctggtcgtaa agcacagaaa cgtcgatcgt agcgaggttt
      121 tctgaatcat cagccagttt cttttgattt ttttctacgt cttcgcgcag aacggtgcct
      181 ttactaaggc ctcgctcagt catgcgaaca tcatattctt tgtctttgat atactgcgaa
      241 agtaaattca tagtgcgccc cttttacccg aatacaatta aaaataccaa taaaaccgag
      301 ggctacaaaa gccccagctg gcccaatcgt tcatttaggt aactgcgggc cgtatgactc
      361 ggcgataatc gtacattccc cgccggatgc aagaagagcg gcatcgaata acgggaggtt
      421 ttcgccaacg ctcctgtcgg gtttactacc cgatgagtgg ttgaaacata gtacccaccg
      481 ctggccatct gaagcatatc cccggaattc acagcaatat cacaggggtc gcattcaacc
      541 tcgtgccagt ttccgtcgag atcctttacc tgaagcccgg gggcggtggc cgccggcagc
      601 aacgtaatta agttaatgtc ttcatgtgcc gccgctcgaa tagcgcccga ctcttcggcc
      661 ccgctcaagg gggggtagtg aatgggacgg aggagcgtgt cgtcgctctc gtcgatcatt
      721 tttcgtagtg gcatcgaaaa gcgcccttta gtttcgggcg gagtgtggtc ttcgacccag
      781 cctaacaact cggcgccgag cccatggagc tgcttgtata gttcaggggt agcccgctgc
      841 gcgagcccag gaagatcacc ccgctttggg tagtagtgaa aaaattcttt caaatcgctt
      901 actttgtagt cttttgcggt ttctgctcga aaaggaaaat aacctgcttg aagcttttta
      961 tcgtaaagaa gctcgtgttt tgacggcgac tcaaagaacc gggcccactc cgaaaaggaa
     1021 tcttcgatca actgatgaga aatgggatgg tggttcaata cggcgaaacc agtttccttt
     1081 agcgaacgtg caaattcttc ggcagcgttt ggacttcggt aatcgacttt caatactcgc
     1141 atgcgaaccc ctttttttga atacgtttac cgccgaaaac ctactccagt caacgacgga
     1201 gcgagctatt tgacctgcaa tcgccaccgg cttatgaagc agggcatgat gtatcgatct
     1261 aaagccctgt tgacgcgcat tctggctgtt caggcattgc ttttgggagt cagtacctcc
     1321 caaactcaag ctaatcccca attccagcga atcaatttgg aacatcttta tgtcggagta
     1381 gagtacacct ttcaaaaccg catgctttta aatgatccgg ccgaaagtcc tcacgacgta
     1441 tcgcccaaaa aataccgact gatgctccga tttttccggg cctaccagca aattttgtct
     1501 gacctaggat tcggtccggt ttcccactct ataggttttc ttggcagtcc ccggggggtc
     1561 ttttaccggg atccccgcac tccaaacgac gaattatttc gcactttagg caccgcctac
     1621 gacagtattt tgcattctga tatgcgtgtg gtcgaggtcg cggaaaaacc caaaaaactt
     1681 acccagctcg aaagtacggc aaatcttatt tatgcggccg ccagcaaagt aaacctaaaa
     1741 ccttatctac tcccgggcgg catgtggagc ggcggagcgc acttcaacat tggatctgct
     1801 cgcctagaag aaagcccatt tattcgtttt ccaaatctgc tgccaaattt actcgcttat
     1861 tttgaccagc acgcttcttt actctatggc ttcggggagg cctttgacgt cggaaacgag
     1921 ggaaccgccg ttccctacca tggctacggc gaggtgtcgc aatttcggag aattcttaaa
     1981 gatttttctg ttcgatatga ggagtttcgt aagatcaaac gcccaggcct acatcagaga
     2041 gtcgctcttc tggcgagctt tctgcgcgat ctaaagattc tcaatcgcca cgaaagtttc
     2101 attaatgtcg aacgactgtt gtcgtccttt agtttcgaag acccccgcaa ccccacgata
     2161 gcgaactcgg gcgatcgcaa ttcgatcgaa atacgaactc tcagaatgcc accaacagct
     2221 gcccatgtgg ctgaattggg ttggttcttt gctcgtcttc tggagaaatt atctgaaccc
     2281 ggccttcttg ttcagcccgt cgccccagtc aattttgata tcatgttaac ggccacagcg
     2341 attgaggccg attggcaact tgtgaaaaaa gacctcaacc ttcaaagccc attgctcgac
     2401 gaaatgatcg acgaacaaac cgcgatgctg caaaccaaaa cctttcgtac tttagatatt
     2461 ccaacagcgg aaattttcag cgccttttcg cccttggcgg aaaaaggaag ggcatttgaa
     2521 atgcgcgttc cgagctatta ctcgaaaaaa atgtccatca atggaaggga acttacgacc
     2581 cagacgctgc agctagggga acaaactttc gaggttgcgt atcttcattc ggatgcattc
     2641 gatatttctc cagaaaaaat aagaaaaaat cgacttcgaa taaattgtca gaatatttta
     2701 ttaccaaacg attaatgaaa tgttggcttt ccgggctctt cgtcgtcgtc ttcttcatcg
     2761 tcttcgaatt cctcgagaac ttcgttattc acaacgagtc gaaggtgact ttttttctta
     2821 cgacgctttc caccgccctc agtagccttg gcgcgccctc gcattcgaaa atacgcgctt
     2881 gcgaataaat ag
//
LOCUS       DAOMXO010000280         2884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19646_length_2884_cov_3.979852, whole genome shotgun sequence.
ACCESSION   DAOMXO010000280 DAOMXO010000000
VERSION     DAOMXO010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19646_length_2884_cov_3.979852"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..656
                     /locus_tag="PLH57_06615"
     CDS             21..656
                     /locus_tag="PLH57_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRK02323.1"
                     /translation="MIQRTRNVSFSFAGQSCISVQRVYVLDGVYDRFTEKLLAATRQI
                     AVGDPAKRGVLAGPVIDDASATRLTEKINAAVTGGAKILIGGEAQAICKRCFEPIVIT
                     NTKPSDAVVRDEIFGPLVCVERVKSLDAGIAAVNNSRFGLQAGIFTDSCQNAKRFADE
                     VEVGGVIVGDIPTLRFDAMPYGGLKDSGVGREGLRSAMEDYSDLKVIVQSV"
     gene            complement(653..1402)
                     /locus_tag="PLH57_06620"
     CDS             complement(653..1402)
                     /locus_tag="PLH57_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRK02324.1"
                     /translation="MSVIRVLVVEDEEAIAQGIIYNFERAGYSVFHIKDGAKALALVL
                     EQNFDLVVLDVMLPHKTGFEILETMRTKRNFTPVLMLTALSDTKERVRGIRLGADDYL
                     GKPFDVEELLARAEALLRRSTWSDTSEPHVKATLSPISLGTIIFDRAAHILRAVPEDK
                     SKSIPLTAMESKLLAALLDQATSSTAPERFIPRTELLSNIWGFQEGTQTRTLDMFIAR
                     LRHYFVELGETNTKIEVLRGVGYRLALGPVS"
     gene            complement(1399..2412)
                     /locus_tag="PLH57_06625"
     CDS             complement(1399..2412)
                     /locus_tag="PLH57_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRK02325.1"
                     /translation="MKAWFNSVQRIRKWASGRPVFWSALAFFVVVTIAVTLSVWWNLL
                     LVEKHQILQRIDNMAQLMADRHEQERVPLALLMFVGIGLSVIAILALVFLFLRLIQTL
                     RLNLAQSQFIAAVTHELRSPVAGIQILFDTLSRGDLPPGKKLEFEASIRKELKRLQDL
                     VDQVLDTATLEHGITKFQMQSLRLNALIHETVEPLATVAASLGGSITCNFNGDPTVYA
                     NRKLLSTALSNILDNAIKYARGIPEITLRTRQTHGWVEIAVEDRGIGLAHGEQRKIFK
                     RFFRAQDARIQAKTGTGLGLYFCKLALSAQGAKISAKSAGPNRGTTFSIRLRAPKEAR
                     LNP"
     gene            complement(2432..>2884)
                     /gene="fusA"
                     /locus_tag="PLH57_06630"
     CDS             complement(2432..>2884)
                     /gene="fusA"
                     /locus_tag="PLH57_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090101.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRK02326.1"
                     /translation="QEGSLIGFPIVGVRVVLNDGSYHDVDSSEQAFKTASIMGFRQAY
                     DKAAPIVLEPIMKLEVQAPEEFQGAVMGQVNQRRGIIMNSQTLEKYFVCEAEVPLTEM
                     FGYSTDLRSATQGKGEFSMEFLKYSPVPRNVQEDMVKAYREKRAKENK"
BASE COUNT          678 a          703 c          787 g          716 t
ORIGIN      
        1 gaaccggcag gtagccagcc ttgatccagc ggaccagaaa cgtgtctttt tctttcgccg
       61 gtcagtcttg tatttctgtg caacgggttt acgttttaga cggtgtttac gatcgattta
      121 ctgaaaaact actggcggcc actcgtcaaa ttgcagtggg tgatcccgcg aagcgaggag
      181 tgctagccgg acccgtgatc gacgacgcgt cggcaacgcg tttgaccgaa aaaataaatg
      241 cagccgtaac cggtggcgca aaaattttaa ttggtggtga agctcaagca atctgcaaac
      301 ggtgtttcga acccattgtg attacgaaca ccaaaccaag cgacgcggtg gtacgcgatg
      361 aaatatttgg tccgcttgtg tgcgtcgaaa gagttaaatc tctcgacgcg gggatcgcag
      421 cggtaaacaa cagccgattt ggtttgcagg ctggaatttt tacggattct tgtcaaaacg
      481 caaaacgttt tgcggacgag gttgaagtcg gcggcgttat tgttggcgac attcccactc
      541 tgcgatttga tgccatgccg tacggaggtc ttaaagattc gggagtgggt cgcgaaggcc
      601 tgcgttcggc gatggaagat tattcagatt taaaggtgat tgttcagtcg gtttaggaaa
      661 ccggacccag cgcgaggcgg tagcctacac cacgtagaac ctcgatcttt gtattcgtct
      721 cgccgagttc gacgaaatag tggcgcagac gcgctatgaa catgtcgagc gtgcgggtct
      781 gggtgccttc ctgaaacccc caaatgttgc tcaaaagttc agtccgagga ataaatcgct
      841 cgggagccgt ggaactcgtg gcctgatcga gcaaggcggc taagagtttc gattccattg
      901 cagttaaagg aatactttta cttttgtctt cgggtacggc tcgaagaatg tgtgccgccc
      961 gatcaaatat aatggttcca agagatatcg gtgaaagtgt tgctttaacg tgcggctcgc
     1021 tcgtgtcgga ccacgtgctt cgacgtagta gcgcctcggc tcgggccagc agttcttcga
     1081 catcaaaggg tttgcctaga taatcgtcgg cgcctaaacg aattcctcgt acgcgttctt
     1141 tggtgtccga gagcgcagtt agcatgagta ccggcgtaaa attgcgtttc gttcgcatcg
     1201 tttctagaat ctcaaaaccc gttttatggg gtagcattac gtctagaacc acgaggtcga
     1261 agttctgctc gagcaccaag gccagggctt tcgccccgtc tttgatgtgg aaaaccgaat
     1321 aacctgctcg ttcgaaattg tagatgatcc cttgggcgat cgcttcttca tcttcgacta
     1381 ccagcacgcg tatgacactc atgggtttag cctcgcctct ttaggggcgc gtagccgaat
     1441 ggaaaatgta gtaccgcgat tgggtccggc gctcttagcg gaaatcttag ctccttgggc
     1501 cgagagcgca agtttacaaa aataaagtcc taggccagtg ccggtttttg cctgaatgcg
     1561 tgcgtcttgt gcccgaaaaa agcgcttaaa tattttgcgt tgctcaccat gggcaaggcc
     1621 gataccgcgg tcttccactg cgatttcgac ccacccgtgt gtttggcgag tgcgtagcgt
     1681 gatctcgggg atcccgcgcg catatttgat agcgttatca agaatatttg aaagtgcggt
     1741 cgatagcagt ttgcgatttg cgtaaacggt agggtccccg ttgaagttgc aggtgatgct
     1801 accacccagc gaagcggcca ccgtggcgag tggttcaacg gtctcatgaa taagagcatt
     1861 gaggcgtagc gactgcatct gaaacttggt tatgccgtgt tcgagcgtcg cagtgtcgag
     1921 aacttggtcg accaggtctt gtaggcgttt tagttctttg cgaatcgagg cttcgaactc
     1981 gagtttttta cctggtggca ggtcgccgcg cgatagggtg tcgaatagaa tttgaatccc
     2041 tgccactggt gaacgcaatt catgagtaac cgccgcgatg aactgactct gggctagatt
     2101 taagcgcaat gtctgaataa ggcgcaagaa cagaaacact agggcgagaa tcgcaatgac
     2161 cgagagccca attcccacga acatgagcag cgcaagaggc actcgttctt gttcatgcct
     2221 gtccgccatg agctgggcca tgttgtcgat tcgttggagg atctggtgct tttcgaccaa
     2281 gagtaaattc caccagaccg acagggtaac cgctatggtc accacaacaa aaaaggccag
     2341 ggcggaccaa aaaaccggtc ggccgctggc ccattttcga atacgttgaa cagaattgaa
     2401 ccaggctttc atgcctgctc aaccgcgata actatttgtt ctccttggcg cgcttctcgc
     2461 gataagcctt caccatgtct tcttgaacgt tgcgaggaac aggagagtac ttcaagaatt
     2521 ccatcgagaa ttcacccttg ccctgggtcg ccgatcgaag gtcggtggag taaccaaaca
     2581 tttccgtaag cggaacttcc gcttcgcaca cgaagtattt ctcgagcgtt tggctgttca
     2641 taatgatgcc acgacgttga ttcacttggc ccatcacagc accttgaaat tcctcaggag
     2701 cttgaacttc aagtttcatg attggctcaa gcacaatcgg tgcggcctta tcgtaggctt
     2761 ggcgaaaacc catgatcgaa gccgtcttaa acgcttgctc cgatgagtcc acgtcatggt
     2821 acgaaccgtc gttgagcacg actcgtacgc cgacgatagg aaagccgata agagagccct
     2881 cttg
//
LOCUS       DAOMXO010000281         2881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19675_length_2881_cov_4.560863, whole genome shotgun sequence.
ACCESSION   DAOMXO010000281 DAOMXO010000000
VERSION     DAOMXO010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19675_length_2881_cov_4.560863"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLH57_06635"
     CDS             complement(<1..334)
                     /locus_tag="PLH57_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRK02327.1"
                     /translation="MEPSQTDCIFCKIVAGTIPAKIIEQNEHAMAFLDLQPQAPFHGL
                     VIPKMHVASLNQITPSERRLILPELYEMADQVAIKHGFREDGYRCVINNQALAGQSVF
                     HLHLHVLGG"
     gene            complement(334..1611)
                     /gene="murA"
                     /locus_tag="PLH57_06640"
     CDS             complement(334..1611)
                     /gene="murA"
                     /locus_tag="PLH57_06640"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRK02328.1"
                     /translation="MDQLVIQGGRKISGIVSARGAKNAALPILFATLLSDRECTIENV
                     PNLADISTTLKILRQMGVETTYDPTTRLATIRAKTVTSPIAPYELVRTMRASVLVLGP
                     LVARFGQAKVSLPGGCSIGARPVNLHLMALEKMGATIEIAEGYIHARCRSLKGAEIEF
                     PQISVGATENTIMAAVLANGRTIIKNAAREPEITDLAKFLNSIGAKIQGAGTATIEIE
                     GVEKLHAGSHRVVSDRIEAGTYLIAGAITEGDVTVENIDEDLLGAFASKLRSSGCDLT
                     KNGTGLRVRGARKILPVSFFTEPFPGFATDMQAQFMALLTMAHGVSVVTENIFENRFM
                     HVAELLRLGAKIAVAGNKATIEGLGDSGRYLSGASVMATDLRASACLVLAGLAAKGET
                     TVKRIYHLDRGYERMEENLTALGAEIRREKDPD"
     gene            complement(1643..2473)
                     /gene="prmC"
                     /locus_tag="PLH57_06645"
     CDS             complement(1643..2473)
                     /gene="prmC"
                     /locus_tag="PLH57_06645"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRK02329.1"
                     /translation="MVTTLIPPHERRILEAHIRKVPLRDLALVTDPLDEYQNRELKEL
                     IRLRMQGLPLQYLTGSQDFFGREFYVNTSVLVPRPETEGLVELAINELSKLKLSTNLT
                     EIRGLDLGTGSGCIAATLALEILGCHVIATDCCSEALGVAAENLRRLRVNNVTLLEVG
                     TPPTLSDYQTLGDLDFIISNPPYLVESDEVATDVRHHEPESALYAPDGQPFLFYEMLI
                     ELGHQKLKNDGVLAFEVPHERADEILQLVEKARFGQARLENDLTGRHRYCVARKTISL
                     "
     gene            complement(2477..>2881)
                     /locus_tag="PLH57_06650"
     CDS             complement(2477..>2881)
                     /locus_tag="PLH57_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRK02330.1"
                     /translation="VFRAGGPGGQSVNTTDSAVRITHIPTGVVVSCQDEKSQHKNKDK
                     AMRILRARILEAEQEKAAALRADERRSMVGTGDRSERIRTYNFPQSRVTDHRIGVTLH
                     QLDDVMEGHIDELINALVSNHQAELLKQESAV"
BASE COUNT          686 a          710 c          703 g          782 t
ORIGIN      
        1 ctccacccaa cacgtgtaga tggaggtgaa aaacgctttg gccggcaagc gcctgattat
       61 tgatcacgca gcggtagccg tcttctctga agccgtgttt gatagccact tgatcggcca
      121 tttcataaag ctccggtaaa attaacctgc gctcacttgg tgttatctga ttcaaactag
      181 ccacgtgcat tttgggaatg accaacccat gaaaaggagc ctgcggttgg aggtccaaaa
      241 aagccatcgc gtgttcgttt tgttcaatta tttttgccgg gatagtacca gcgacgatct
      301 tacagaaaat gcagtcagtc tggcttggtt ccattagtct gggtcctttt ctcgccgaat
      361 ttcggctccg agggcagtaa gattctcttc catgcgctcg tagcctcggt ccaaatggta
      421 aatacgttta accgtggttt cacccttggc cgctaagccg gcgagcacca aacaggcgct
      481 cgcgcgcagg tcagttgcca tcacggatgc ccctgaaaga tagcgtccgg agtcgcctag
      541 cccctcgatt gtagccttgt ttccagcaac tgcgattttt gcacccaatc gcagtaattc
      601 cgcaacatgc atgaatcgat tttcgaaaat gttttccgta accactgaaa cgccgtgagc
      661 catggtcaaa agcgccatga actgggcttg catatctgtg gcaaagccag gaaatggttc
      721 cgtgaaaaag ctaacaggta atatttttct ggctccacga acgcgcaggc ctgttccatt
      781 tttagttaga tcgcaccccg agcttcggag tttggaagca aaggccccaa gtaggtcttc
      841 gtcgatattt tccactgtaa cgtcgccttc agttatggcc cccgcgatta agtaggtccc
      901 tgcttcgata cggtctgaaa ctactcggtg acttccggcg tggagttttt caactccttc
      961 tatttcgatt gtagctgttc cagcgccctg aattttagcg cctatcgaat tgagaaattt
     1021 cgcgaggtct gtaatctctg gttcgcgtgc tgcattttta ataatggttc tgccattggc
     1081 aagaaccgcg gccataatag tgttttcggt agcgccgacc gaaatttgag gaaactcaat
     1141 ttccgccccc ttaagtgacc tacaacgggc gtgaatataa ccttccgcga tttcgatcgt
     1201 ggcgcccatt ttttctaatg ccatcaagtg aaggttcact ggccgagcac ctatgctgca
     1261 accaccagga agcgacactt ttgcctgtcc gaaacgagcg acaagaggac ctagaaccaa
     1321 aacagaggcg cgcatcgttc ttactaattc gtagggtgcg atcggactgg tgaccgtttt
     1381 tgcgcgaatg gtggccaacc gagttgtggg atcgtaggtg gtttcaactc ccatttgcct
     1441 cagaattttt aaggtagtgg agatatctgc aaggttcggg acattttcga ttgtgcattc
     1501 tcgatctgac aatagcgtcg caaataagat cggaagagcg gcattttttg cgccccttgc
     1561 actgacgatt ccagaaatct tccggccacc ttgaatgacg agttgatcca taagccctta
     1621 aagtatcgat cagcgatacg agttacaagc tgatagtttt tcgggccaca cagtaacggt
     1681 ggcgacccgt tagatcattt tccaaacgag cttgaccgaa tctagctttt tctacgagct
     1741 gaagaatttc atcagctcgt tcgtggggaa cctcaaatgc tagaacgcca tcatttttaa
     1801 gcttctgatg gcctaattca atgagcattt cataaaagag aaagggttgg ccgtcggggg
     1861 cgtacaaggc tgattccggc tcgtgatgtc ttacgtcggt ggcaacttcg tcgctttcaa
     1921 ctaaataagg cggattgcta attataaaat caaggtctcc aagagtttga taatcgctaa
     1981 gtgtcggagg ggtgcctact tctaataatg tgacattgtt cactctaagt cgccgcaagt
     2041 tttctgcggc aactccgagt gcttcactac agcagtctgt cgcaataacg tggcaaccca
     2101 gaatttcgag tgctaaagtc gcggcgatgc aaccagagcc cgtgccgagg tcgagtcctc
     2161 gtatttcggt aaggtttgta gaaagtttga gtttagaaag ctcattaatt gcaagttcta
     2221 cgagtccttc ggtttcgggt cgtgggacca aaaccgaggt gtttacataa aactcgcgcc
     2281 caaaaaaatc ttgagatccc gttaagtact gaagcggcaa tccctgcatt cgtaaacgaa
     2341 tcaactcttt aagttcacgg ttttgatatt catcgagcgg gtcagttacc agcgccaaat
     2401 ctcggagcgg tactttgcga atatgagctt cgagtatgcg tctttcatgg ggtggaatta
     2461 gcgtggtaac cacaagtcaa actgcacttt cttgtttgag cagttccgct tgatgattcg
     2521 agaccaacgc attgatcagt tcgtcaatgt ggccttccat tacatcgtcg agttggtgca
     2581 gagttactcc aatgcggtga tcggttaccc gagactgcgg aaagttgtag gttcgaatgc
     2641 gttcgctgcg atcgccagta ccgaccatgc tacgtcgttc gtcagctcga agagccgctg
     2701 ctttttcctg ttcagcctca agaatacgcg cccgtaaaat gcgcatggct ttgtctttgt
     2761 tcttgtgttg gctcttctca tcttggcacg aaacaaccac tccagtcggt atgtgcgtaa
     2821 tacgaactgc cgaatcggtt gtatttaccg actgtccacc cgggcctcca gcgcgaaaca
     2881 c
//
LOCUS       DAOMXO010000282         2878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19704_length_2878_cov_5.721927, whole genome shotgun sequence.
ACCESSION   DAOMXO010000282 DAOMXO010000000
VERSION     DAOMXO010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19704_length_2878_cov_5.721927"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2354
                     /locus_tag="PLH57_06655"
     CDS             <1..2354
                     /locus_tag="PLH57_06655"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRK02331.1"
                     /translation="SVAILAIVLLNGILGFVQEYRAERAMEALQNLVAPQTKVRRHGE
                     IHVVPSYELVPGDIVLLEAGNLVPADLRLVELASLEINESALTGESVPVGKVPHVLAS
                     ERAAVGDQKNMAFKGTIVSAGRGTGVVVRTGMGTELGKIANLLKKEVEVETPLQKKLA
                     RFAKNIAFLVVALCAVMFAVGLARGNAPVLMFLTSLSLAVAAIPEALPAVLSVALALG
                     AREMSKRNALVRKLSAVEALGSVTSICSDKTGTLTENRMTVGEVRAVAPESNDLLFRM
                     LALSNDASVGANGALQGDPTETALLAAALEAGFDKTQLEKECPRIAEIPFSAERGMMT
                     TVHQVGHERLVFTKGAPERVFKQCINSVAGEFRAAAAESTAAELARQGFRVLAFAYAR
                     KPGDSTVIDLGTVETGLTYVGLIGLMDPPRCEAKEAIELCFKAGIRPVMITGDHPATA
                     SAIAEKLGLLVEGRSEVVTGEQVSKMTEQELKSRVNTIAVYARVAPEQKIQIVKVLQA
                     LGEVVAMTGDGVNDAPALKRADVGVAMGRGGTDVAREASHLILLDDNFATIVAAVREG
                     RRIYDNIRKFVRFALSGNSGELWTLFLAPFFGLPTPLLPIQILWINLVTDGLPGLALA
                     FEPEEKDIMQRPPRDPKESIFSRGLWQHSLWVGLLTAGVTLGTLAYAYRTGNERWQTM
                     AFTVLSLTQMGHVLAIRSERRSLFKIGLFSNTYVLGAVVLTIGLQLLCLYTPFLRSVL
                     GTTALTASELAWCFGLSSVVFVAVEVEKWFQRRFARFGRISAL"
     gene            complement(2312..2800)
                     /locus_tag="PLH57_06660"
     CDS             complement(2312..2800)
                     /locus_tag="PLH57_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRK02332.1"
                     /translation="MEVPKGSKVKYELDKQSGLIKVDRVLFSSVHYPANYGFIPQTYC
                     DDHDPLDILVLGQEAVVPLSIMIAKPIGVMKMLDQGEADDKIIAVHAHDPEYSHYNSI
                     DELSPHRMMEVKRFFEDYKALEQKSVVVEKFLGPKEALKVIADSIQLYNAEIRPKRAN
                     RR"
BASE COUNT          656 a          678 c          818 g          726 t
ORIGIN      
        1 atagtgtcgc gattctagca attgttttgt taaacggaat tttgggtttc gtgcaagagt
       61 atcgcgcgga acgcgccatg gaggcactac aaaacctggt agcgcctcag accaaggtaa
      121 gaagacacgg agaaattcat gtggtgccgt cttacgaatt ggttcctggc gatatcgtgt
      181 tgctcgaggc cggaaacttg gttccggccg acttgcgtct ggtggagctg gcttcactcg
      241 aaatcaacga atctgccttg accggcgagt cggtgcccgt cggaaaagta ccacacgttc
      301 tggctagtga gcgcgccgca gtgggcgatc agaaaaatat ggcctttaaa ggtacgatcg
      361 ttagcgccgg ccgcggtacc ggagtggtcg tacgcactgg aatgggcacg gaactcggaa
      421 agatcgcgaa tcttttaaaa aaagaagttg aggttgaaac tcctcttcaa aagaagcttg
      481 cacgttttgc taagaatatt gcgtttctgg tagtggcctt gtgcgccgtt atgtttgcag
      541 tcggacttgc ccgaggcaat gcgccggtat tgatgttttt gacgtcgttg agtctggccg
      601 ttgcagccat tcctgaagcg ctcccggcgg tactttcggt agcgctcgcg ttgggcgcgc
      661 gcgaaatgag taagcgaaac gcacttgttc gaaaattatc ggcagtcgag gcgttagggt
      721 ccgtgacttc gatctgttcc gacaagactg gcactttaac tgaaaatcgc atgacggttg
      781 gtgaagtgcg ggcggtagcg cccgaaagta acgacttatt gtttcgtatg ctcgcgttga
      841 gcaacgacgc cagcgttgga gcaaacggcg cattacaggg cgacccgacg gagacggcac
      901 tgcttgccgc agcactcgaa gcgggctttg acaaaactca acttgaaaaa gagtgtcccc
      961 gaatcgcaga gatcccgttt tctgccgaac gcggaatgat gaccaccgtt caccaagttg
     1021 ggcacgagag actggtgttt accaagggcg caccggagcg ggtgttcaaa cagtgtataa
     1081 atagtgtggc cggtgagttc agagcagccg ccgccgaaag tacagccgcg gagctagcgc
     1141 gccaagggtt tcgtgttttg gcttttgcct atgcccgcaa acctggcgat tcgactgtca
     1201 tagatcttgg aactgtggag accggtctaa cctacgtggg tctaattggt ttaatggatc
     1261 caccacgatg cgaggctaaa gaggccatcg aactttgttt taaggccggt attcgcccgg
     1321 tcatgattac gggagatcac cctgctacag ccagtgcaat cgccgaaaaa ttagggctgc
     1381 tagtcgaagg gcgctcggaa gtcgtgactg gcgaacaagt ttctaaaatg accgaacaag
     1441 aactcaagtc acgggtaaat accattgcgg tttatgctcg tgttgcgccc gaacaaaaaa
     1501 tacagatcgt gaaggtctta caggcgttgg gcgaagtcgt ggcgatgacg ggcgacggag
     1561 tcaacgatgc tccggctctc aagcgtgccg acgtgggagt cgccatgggc cgcggtggca
     1621 ccgatgtcgc ccgcgaagcg tcacacctaa tattattaga tgataatttt gccaccatcg
     1681 tagcggccgt tcgcgaaggg cgaagaatat acgacaacat acgcaagttc gtgcgatttg
     1741 cattgtcggg caactccggc gaattgtgga ccttgttttt ggcaccattt tttggtttgc
     1801 cgaccccact gctgccaatt caaatattgt ggatcaatct agtgaccgat gggctacctg
     1861 gattggcctt ggcatttgaa cccgaagaaa aagacatcat gcagcggcca ccacgcgacc
     1921 caaaggagag cattttttcc cgcggcctgt ggcaacacag tttgtgggta ggattactca
     1981 cggccggagt gacgttggga acgctggctt acgcctatcg gactggtaat gaacgctggc
     2041 aaacaatggc attcacggtg ttgtcgctca ctcaaatggg gcacgtgctc gcgattcgct
     2101 cggagcgccg ctcacttttt aaaattgggt tattttccaa tacttatgtt ctaggggcgg
     2161 ttgttttaac aattgggctt cagctgttgt gtctatacac gccatttctg cgatcggttt
     2221 taggcacgac cgcgcttaca gctagtgaac ttgcctggtg ttttggtttg tcgtcggtgg
     2281 tgtttgttgc cgttgaagtt gaaaaatggt ttcagcgtcg attcgcgcgt ttcggtcgaa
     2341 tttcggcatt ataaagctga atcgaatcag caatgacttt gagtgcctct ttggggccaa
     2401 gaaatttttc taccaccacg gatttttgct caagtgcttt gtagtcttca aaaaaacgtt
     2461 tcacttccat catgcgatgc ggtgatagtt cgtcgatcga attgtaatga gaatattccg
     2521 gatcgtgtgc gtgcactgca atgattttgt cgtcggcctc gccttgatcc agcattttca
     2581 taacgccgat cggtttggcg atcatgattg aaaggggcac cacggcttct tgacctaaaa
     2641 ccaaaatgtc gagcgggtcg tggtcgtcac aataagtttg cggaatgaaa ccgtagttag
     2701 cagggtaatg cacgctacta aaaagtacgc gatcaacttt aattagtccg ctttgcttgt
     2761 ctagttcgta ttttactttg gaacccttgg gtacttcgat gactgccgca actgctttgg
     2821 gggcggtact gccgctttgc cctggtgcga gctggcgaga ccgcggcgac ggacagcc
//
LOCUS       DAOMXO010000283         2876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19725_length_2876_cov_4.129741, whole genome shotgun sequence.
ACCESSION   DAOMXO010000283 DAOMXO010000000
VERSION     DAOMXO010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19725_length_2876_cov_4.129741"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PLH57_06665"
     CDS             complement(<1..679)
                     /locus_tag="PLH57_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HRK02333.1"
                     /translation="MDTTAKDIQFDENGICNYCNEFLLRSGHILEKKSEERERERTSL
                     VNEIKKRGTGKSYDCIVGVSGGVDSSWVLVQAVNLGLKPLAVHMDNGWNSELAQNNIY
                     NLIQKLGVDLYTHVIDWTEYRALMQAFFEADVVDVELLYDNAMLAVNYKMAARQGVQF
                     ILGGTNQATEGMRMPPDWNWFKFDKRNIKAIGQKFSKQRLRTFPAMGTFDYIYYEFLK
                     KIHWVSFL"
     gene            complement(729..1487)
                     /locus_tag="PLH57_06670"
     CDS             complement(729..1487)
                     /locus_tag="PLH57_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HRK02334.1"
                     /translation="MLTHRLIPVLLLKDGGLVKTTKFGDPKYVGDPINVVRIFNEKEV
                     DELAILDIFASSRGRAPDFELLAQIASECFMPLSYGGGIRSIDDAKRVFSLGIEKVCI
                     QTAALQNIKIITEIARDFGTQSITVSVDIKKNWLGRRGLYSASAGKIIQGDWLNYLKT
                     LEAAGAGEILLTSVERDGTLSGMDFELIREVSECLSIPVVAAGGASSLDDVRKAIDCG
                     ANAVAAGAFFVYQGPHRAVLISYPRYAELKRLMG"
     gene            complement(1492..>2876)
                     /locus_tag="PLH57_06675"
     CDS             complement(1492..>2876)
                     /locus_tag="PLH57_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRK02335.1"
                     /translation="LPGWSAKLQHQRAYEYLNFGLTDHTEETLFSGVRQVRGGEMITL
                     SMADACGEIRPVRWYELKPTAVRFDYAEAVDYFRELFFDAVRLRLRSDVPVGTALSGG
                     LDSSSIVCAVHHLLNHSSKKDFQNTFSACSKIKRFDESRFINSVVQKTNVKPHYTQPS
                     LSELFLELEKIIWHHDEPFASTSVFAEWKVFELVAQTDVKVTLDGHGADEMLAGYHGY
                     FIPFYMNLLLRGQFFTLARELQAARRNHGRANHHLAQALFRLLVPAPARSFVKKLVSH
                     GVSHTDWLNGNVLKSNATNPFETYGGMARSIREFSYDQMLHSSLPKQLKWADRDSMAH
                     SIESRAPFLDYRLVEFLLGCPDSFKIASGTTKRILRGAMRGFLPELVENRPDKMGFVT
                     PEEHWVQNEAPAQFLEEARRTIDSSHEVFTSTALEQIEAVVSGKKTFNHQPWRIIAFG
                     AWMKRFSVGV"
BASE COUNT          801 a          718 c          570 g          787 t
ORIGIN      
        1 ccaaaaatga gacccaatga atttttttta aaaattcgta atagatataa tcgaaagttc
       61 ccatcgccgg aaaagtgcgt aatctttgtt ttgaaaactt ctgtccaatc gcctttatat
      121 tgcgtttatc gaacttgaac cagttccaat ctggaggcat acgcatgcct tctgtcgcct
      181 gattggtgcc tccaagtata aactggacac cctgcctagc cgccatttta tagttcactg
      241 ctaacatggc gttatcgtaa agaagttcaa cgtcgacaac atcggcctcg aaaaaggcct
      301 gcataagcgc tctatactcc gtccaatcga tgacgtgggt gtaaagatcc actccgagct
      361 tttgaattag attataaata ttattttgtg ccaactctga attccagccg ttgtccatat
      421 gcaccgcaag tggctttaat cctaggttta ctgcttgaac gagcacccac gaactgtcta
      481 caccaccaga tacgccgaca atacagtcgt aagatttacc agtgcctctt ttttttatct
      541 cgtttactaa agaggtccgt tcacgttcac gttcttcgct ttttttctca aggatatgac
      601 cagagcgtaa gagaaattca ttacaataat tgcaaattcc attttcgtcg aactgaatgt
      661 cttttgcggt tgtatccatt atgcaacgct tacatattcg aaagtttttt tcgtgtttca
      721 caaaacaatc atcccattaa tctctttaac tcggcatacc ttggataact aattagcacc
      781 gctctatggg gaccttgata aacgaaaaaa gcacccgcag ctactgcatt tgccccacaa
      841 tcaatagctt ttctcacgtc atccaaacta gaagctccac cagcggccac aactggaata
      901 gataaacact cggacacttc gcgtatgagt tcgaaatcca ttccgctcaa ggttccgtct
      961 cgttccaccg aagttaacag aatttcacca gctccggctg cttcgagggt tttgagataa
     1021 ttcaaccaat ccccttgaat tattttcccg gccgaggcgg aatacagccc gcgtctcccc
     1081 agccagtttt ttttaatatc cacggaaaca gtaatacttt gggtaccaaa gtctctcgca
     1141 atttcagtga taattttaat attctgaagt gctgcagttt gaatacatac cttttcaatg
     1201 cctaacgaaa agactctttt tgcatcatcg atcgaacgaa ttcctcctcc gtaggacagc
     1261 ggcataaaac actcactggc gatctgtgct aaaagttcga aatccggtgc ccgacctcta
     1321 gaactcgcga aaatatccaa tatcgctagt tcgtcgactt ctttttcgtt gaaaattcga
     1381 acgacattga ttggatctcc aacatatttt ggatcaccaa atttagttgt ttttactagg
     1441 ccaccgtctt tgagaagcaa tactggtatt agtcgatgag ttaacataac gttacacacc
     1501 taccgaaaat cgcttcatcc aggccccaaa agcaataatt cgccaaggct ggtggttgaa
     1561 agttttcttt cccgaaacga cggcctcaat ctgctcgagt gccgtcgaag taaatacctc
     1621 gtgtgaacta tcgatcgttc tccgggcctc ttctaaaaac tgagccggtg cttcgttctg
     1681 cacccaatgt tcttcgggcg taacgaagcc catcttgtcc ggtcgatttt ctaccaattc
     1741 aggcaaaaaa cctcgcatcg cgcctctcaa aattcttttg gtagtaccgg aagcgatttt
     1801 gaacgagtcg ggacaaccaa gtagaaactc tactagccgg taatccagga aaggcgcccg
     1861 actttcaata gagtgggcca tagaatcacg gtccgcccac tttaattgtt tcggaagact
     1921 agaatgaagc atttggtcgt acgaaaactc acgaatcgaa cgcgccatgc ccccgtaggt
     1981 ttcgaacggg ttagttgcat ttgatttcaa gacgttgccg tttagccaat cagtgtgaga
     2041 cactccatga ctgaccagtt tttttacaaa actccgcgct ggcgccggaa ccaataaccg
     2101 aaacaatgcc tgagccaaat gatggttggc tcgaccgtga ttacgcctcg ctgcttgaag
     2161 ctctcgcgcc aacgtgaaaa attggccccg aagtaaaaga ttcatataaa aaggaataaa
     2221 gtatccgtga taccccgcaa gcatttcgtc agcgccgtgt ccgtccaagg ttactttcac
     2281 gtcggtttgc gctaccagtt caaagacttt ccattccgca aagacactcg tcgatgcaaa
     2341 cggttcgtcg tgatgccata tgattttttc aagctctaaa aataattcac taagcgacgg
     2401 ttgagtgtag tgcggtttaa cgttggtttt ctgaaccacc gaatttataa accgactctc
     2461 gtcaaagcgc tttattttag aacacgccga aaaggtattc tggaaatctt ttttcgatga
     2521 atgatttaaa agatgatgaa ccgcacagac aatcgaagat gaatcgagac ctccggaaag
     2581 cgctgtaccc acaggaacat cggagcgaag gcgtaaacga acggcatcga aaaataattc
     2641 cctgaagtaa tctaccgctt cagcgtaatc aaatctaaca gcggtaggct tcaactcgta
     2701 ccaccggaca ggtcggattt caccacaagc gtctgccata gagagagtga tcatttcccc
     2761 ccctctgact tgccgtacgc cggaaaaaag ggtttcttcg gtatgatccg taagcccaaa
     2821 atttaaatac tcgtatgctc tctggtgttg cagtttagct gaccagccgg gcaaca
//
LOCUS       DAOMXO010000284         2866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19798_length_2866_cov_4.774102, whole genome shotgun sequence.
ACCESSION   DAOMXO010000284 DAOMXO010000000
VERSION     DAOMXO010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19798_length_2866_cov_4.774102"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..314
                     /gene="ccoS"
                     /locus_tag="PLH57_06680"
     CDS             156..314
                     /gene="ccoS"
                     /locus_tag="PLH57_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRK02336.1"
                     /translation="MTILAILMPLALLLSFSFTAVYFWSVWRGQYDDLETPAHRILLE
                     DEQEGKIS"
     gene            311..2446
                     /gene="ccoN"
                     /locus_tag="PLH57_06685"
     CDS             311..2446
                     /gene="ccoN"
                     /locus_tag="PLH57_06685"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRK02337.1"
                     /translation="MTSTESNKLETFYYDDGVVKKFLLATVLWGVVALLLGVVVAVQL
                     SDWRFNFNTSWLTFGRLRPLHTNAAIFAFAGNAIFAGMYHSSQRLLKARMFSDLLSQI
                     HFWGWQLIIVAAAITLPLGITVSKEYAELEWPIDLAITFVWVVFAVNFVGTMIKRREK
                     HMYVAIWFYIATIVTVAVLHVVNSIALPVSFWKSYSVYAGVQDALVQWWYGHNAVAFF
                     LTTPFLGLMYYYVPKAAGQPVYSYRLSIIHFWSLVFIYIWAGPHHLLYTALPEWAQTL
                     GMVFSVMLIAPSWGGMINGLLTIRGVWDRVRKDPILKFFVAALTFYGMATFEGPLLSV
                     KSISALGHYTDWIIGHVHGGTLGWNGFMIAAMLYYVVPKIWKTRLYSERLANIHFWVG
                     FLGILMYVVSMWIAGITQGLMLRSITPAGQLAYPDFIETVVRLIPLYWVRATGGTLYL
                     VGFLILGYNILRTVAQAPKSAPEAQTAPALAKLNPNDLLKGRPHRHLEAMPAVFTVLT
                     LLAVLVGSIIEIMPTLMAHNYVGLTQKTLPYRPLELLGRDIYVKEGCYTCHSQMVRPL
                     PSEVLRYGKRTTAEDSMYDFPHQWGSKRTGPDLANLGGKFPDLWHYRHMLDPREMSPK
                     SIMPAYPWLAIEKVDFKGVTKKMKVMKSLGVPYSEAEIQYGESLARDHAKQIASQLAT
                     QGVPATVEDREIVALIAYLQRLGRAGGVQ"
     gene            2443..2622
                     /locus_tag="PLH57_06690"
     CDS             2443..2622
                     /locus_tag="PLH57_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HRK02338.1"
                     /translation="MKSEVLSSFSMPWLSGLGLILFFTVFVGAIGWVFRRSGRDVYAS
                     MAELPFSDENGGNKI"
     gene            2619..>2866
                     /locus_tag="PLH57_06695"
     CDS             2619..>2866
                     /locus_tag="PLH57_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRK02339.1"
                     /translation="MSEDNRNQSNTGDKHEYDGIVELDNQLPRWWRNLFYATIIFSII
                     YVWHYEFGAGPSIQDEFNAQMNALELQRLAKSSGAGLS"
BASE COUNT          655 a          644 c          758 g          809 t
ORIGIN      
        1 gcagcgggag cactcgcgat ttctggccac atgacaccgc tgctggcagc tttgttcatg
       61 cccgtgagtg cctttactgt ttttgcttcg actttatata gtagtcaaaa aaatgaaaaa
      121 attgcgttta aaaaaatgca gttggaggcc agaacgtgac tattcttgcg atactcatgc
      181 cgcttgcgtt gctgctctca tttagtttta cggcagttta cttttggtct gtttggcgtg
      241 gtcagtacga tgatctcgaa acacccgctc acagaatttt actcgaggat gaacaggaag
      301 gaaaaatatc atgactagta cggaatcgaa caaactcgaa acattttatt acgacgacgg
      361 cgtagtaaaa aaattcttgt tggcaacggt actttggggg gtagtggctc tacttctcgg
      421 ggttgtggtc gcagtgcaac tttctgactg gcgttttaat tttaatacgt cctggcttac
      481 tttcgggcgg ttgcggcccc tccacaccaa cgcagccatt tttgcttttg cgggaaatgc
      541 cattttcgct ggcatgtatc attcgagtca gcggctgctc aaggcgcgca tgttttccga
      601 tttgctttcg cagatacatt tttggggatg gcagcttatt attgtggccg ccgcaattac
      661 gcttcctttg ggaattactg taagcaaaga atacgccgag ttggaatggc ccatcgattt
      721 agcgatcacc tttgtttggg tcgtgtttgc ggtgaacttc gttggcacca tgattaagcg
      781 tcgcgaaaaa cacatgtacg tggcgatctg gttttatatc gcgaccattg tgacagtcgc
      841 cgtgctacat gttgtaaact caatcgcttt gccagtttct ttttggaaaa gttattcggt
      901 gtacgctggt gtgcaagatg cgttggtaca gtggtggtat ggtcacaatg cggtcgcatt
      961 ttttcttacg accccgtttt taggtttaat gtactattac gttcctaaag cggcgggcca
     1021 gccggtatac agttatcggt tatctataat tcacttttgg tcgttggtgt ttatttacat
     1081 ctgggccggc ccccaccatt tgctttacac cgctttgccg gaatgggcgc aaactcttgg
     1141 aatggttttc agcgttatgt tgattgcacc tagctggggc ggaatgatca acgggttgct
     1201 aactattcga ggggtctggg accgagtgcg caaagacccg attctcaagt ttttcgttgc
     1261 agctctaact ttttatggaa tggcgacctt cgaaggaccg cttctttcgg ttaaaagtat
     1321 tagcgcactt gggcattata cagattggat cattggacac gttcacggag gcacgctcgg
     1381 ttggaacgga ttcatgatcg ctgccatgtt gtattacgtg gtaccgaaaa tatggaagac
     1441 acgactctac agcgaacggt tagcgaatat ccatttctgg gtcgggtttt tagggatcct
     1501 aatgtacgtg gtttcaatgt ggatcgcggg cattactcaa gggctaatgt tgcggtctat
     1561 tactccggca ggtcagttag cgtaccctga ttttattgaa accgtggtgc gactaattcc
     1621 gctttattgg gtgcgagcaa caggtggtac gttgtacttg gtcgggtttc tgatcctggg
     1681 atacaatatc ttgcgtaccg tagcgcaagc gccgaaatct gctcctgagg ctcagaccgc
     1741 accggcgtta gcgaaactca acccgaacga cttactaaag gggcggcccc atcgacatct
     1801 cgaagcgatg ccggcggtgt ttaccgtgct cactttgtta gctgtgctcg tgggttcaat
     1861 tatcgaaatc atgccgaccc taatggctca taattacgtg gggctcactc aaaagacgct
     1921 gccatatcgg ccgctggagt tgttgggccg tgatatctac gtgaaggaag gctgctatac
     1981 ttgtcactcg caaatggtgc gtccgttgcc ctcagaagtg ttgcgctacg gtaaacgtac
     2041 gacagcagaa gactccatgt acgactttcc tcatcaatgg ggctccaaac gaactggtcc
     2101 ggacttggcg aacctcgggg gtaaattccc ggatctttgg cattatcggc acatgctcga
     2161 tcctcgggag atgtcgccaa aatccattat gccggcctat ccatggctcg cgatcgaaaa
     2221 agtggatttt aaaggcgtga ccaaaaaaat gaaggtcatg aaaagtttgg gtgttccgta
     2281 tagcgaagcg gaaattcagt acggagagtc cctcgcgcgt gaccacgcca aacagatcgc
     2341 tagtcagctg gccacgcagg gagtgcctgc cacagtggaa gatcgggaaa ttgtagcttt
     2401 gattgcgtat ttgcagcgcc ttgggcgcgc gggaggtgtg caatgaaatc ggaggtatta
     2461 tcttcatttt caatgccatg gctctcgggt ctgggcttaa ttcttttttt tacagtattt
     2521 gtcggcgcga tcggctgggt ttttcggcgc tcggggcgtg acgtctacgc gagcatggcc
     2581 gaactcccat ttagcgacga gaacggaggt aacaaaatat gagtgaagac aatcgtaacc
     2641 aatctaatac cggtgacaaa cacgagtacg acggaatcgt cgaactcgac aatcaattgc
     2701 cacggtggtg gcgcaatttg ttttacgcta cgatcatctt ttcgataatt tacgtctggc
     2761 actatgagtt tggtgcgggt cccagtattc aagacgaatt taatgcccaa atgaatgcac
     2821 tggaattgca acgccttgcc aaaagttccg gtgccggatt gtcgga
//
LOCUS       DAOMXO010000285         2856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_19908_length_2856_cov_4.624777, whole genome shotgun sequence.
ACCESSION   DAOMXO010000285 DAOMXO010000000
VERSION     DAOMXO010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19908_length_2856_cov_4.624777"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLH57_06700"
     CDS             <1..836
                     /locus_tag="PLH57_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02340.1"
                     /translation="WTSEANIQAQRSIFFRFFGLGSRTLEADETSFTRLYGQAMLRHG
                     YSFTKHWNAGLTFAAQRILTESIGVSGFPKTHDRFVRQKGLAPSTLLSPGLSFRFDSR
                     AQKEYSTRGTFIESSVQHNAGLAGSSDFLRTSLEARTLWEEIGQTLTGAARLSWTYAG
                     GPRGDIPFHYLSTLGGAYRMRGFTENRFYDRGAWSLEIEQRFAMFQTNIYDVIADWRV
                     DPFVAIGQVYDGSGDMFDHVQIAAGLGFRAWVRPNVLGRVDTAIAGEGLKIYVELGYP
                     F"
     gene            complement(855..1829)
                     /locus_tag="PLH57_06705"
     CDS             complement(855..1829)
                     /locus_tag="PLH57_06705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK02341.1"
                     /translation="MPRVSILTALYNHEKFVANAIESVLAQTYTDWELILWDDGSTDK
                     SMEVAQKYLERHPGKITLYAHANGVNLGQERTRNAALGRAGGEYVALLDSDDFYHARK
                     LELLVPLLDDPEVGLAYGDVRHVEWSTGREFASGAKNSGESGTVLNALFEENFLGAAA
                     TLIRRSALPNNTPFDSRFKTCGEYPLWIEIAKNWKFAYTPHPVAYWRVHGENTSLKFA
                     VQAKKELLDLFQLLKSDVRYVDQAKAIQRQIWKRKYDYASEAYSAGMFEVARSEFQEV
                     ASATEASRKLRTKSRILLGTLKLGEPANLFLSRAKKRLWSIRNPRAGV"
     gene            complement(1840..2253)
                     /locus_tag="PLH57_06710"
     CDS             complement(1840..2253)
                     /locus_tag="PLH57_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRK02342.1"
                     /translation="MDEKLVEYKKGDVLFHEGENSKDLYIVQTGVVKIFKECDGRKLP
                     IALVHAGQFVGELSFFDGKPRSASAECATDAKILTLNQAHLEREMKKLPSWLLVMIRS
                     IADRMRDTDEIVKRNRICDKTMEQEFSRWGDGQPK"
     gene            complement(2274..>2856)
                     /locus_tag="PLH57_06715"
     CDS             complement(2274..>2856)
                     /locus_tag="PLH57_06715"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02343.1"
                     /translation="HEFKMKVVPVLDDEFAKKFKCETALELRKKVVESLEEERAKNSK
                     EAIKENLLKALIDANTFEVPSALVRTQSEHLMRENFNVLKRQGFTEKMIRDYLEKNSK
                     ELETRAGEQVKAALILQKIAEQQDIKVTDADLDVEFAKMAEPMKSSPEEIRKMYESHE
                     DSLRQLKFRLREERTVDYALSQVKIQDEGKKKK"
BASE COUNT          676 a          748 c          640 g          792 t
ORIGIN      
        1 aatggaccag cgaagctaac atacaggctc agcgaagcat tttttttcgg ttttttggct
       61 taggctctcg cactcttgag gcagacgaaa cgagcttcac gcgcctgtac gggcaagcga
      121 tgttacgcca cggctacagt ttcacgaaac attggaatgc tggacttact ttcgcagcgc
      181 aacgaattct tacggagagc atcggtgttt cgggatttcc gaaaacccat gatcgattcg
      241 tgcgacaaaa gggtttggcc ccttcgactt tactgagccc cggtctctcg tttcgattcg
      301 attctcgggc ccaaaaagaa tattccactc gcggaacgtt tattgagtcc tcggtccagc
      361 acaatgctgg tttggcagga agtagcgact ttcttcgtac gagcttagaa gcccgcaccc
      421 tttgggagga aatcgggcag acactcaccg gcgcagcgag actttcatgg acctacgccg
      481 gggggccccg cggcgatatt ccgtttcact acctgagcac actcggaggg gcctaccgca
      541 tgcggggttt cactgaaaac cgcttctacg atcgcggcgc ttggagttta gaaatcgaac
      601 agagattcgc aatgtttcaa actaatattt atgacgtaat cgccgactgg cgcgtcgacc
      661 cgtttgtcgc aatcggccag gtatacgacg gcagcggcga tatgtttgat cacgtgcaaa
      721 tcgcggcagg cttaggattt cgtgcgtggg ttcgtcccaa cgtattagga agagtcgaca
      781 ccgcgatcgc aggcgaaggg ctcaaaattt acgtcgagtt gggataccct ttttaacttc
      841 gttgaaacaa aaggctaaac gccggcgcgg ggattacgaa tagaccaaag cctttttttt
      901 gctcggctca aaaaaagatt cgccggttcg ccaagcttca aagtgccaag taaaattcga
      961 gacttcgttc gcaactttct actcgcctcg gttgcgcttg caacttcctg aaattcactt
     1021 ctcgcaactt caaacatacc ggccgaatag gcctccgaag cgtaatcgta ttttcgtttc
     1081 cagatttggc gctgaatcgc tttcgcttgg tcgacatacc gaacgtcgga cttcaatagc
     1141 tggaataaat ctaatagttc cttcttggct tgaacggcaa atttaaggct cgtgttctcg
     1201 ccgtgcaccc gccaataagc taccgggtgc ggtgtgtagg caaatttcca atttttggct
     1261 atttcgatcc atagcggata ttcgccacag gttttaaaac gactgtcaaa cggagtattg
     1321 ttcggaagcg cgcttcggcg aatcaaggtg gccgccgccc ctaggaaatt ctcttcgaac
     1381 aaggcattta atacagttcc gctctctcct gaattttttg cgcccgatgc gaactctcgt
     1441 cccgtgctcc actccacgtg ccgcacatct ccgtaggcga gcccgacttc cgggtcatca
     1501 agaagtggca ccaacagttc aagcttacgg gcatggtaaa aatcatcgga atcgagcagt
     1561 gctacgtact cgccgcccgc acggcccaag gccgcattcc tcgttcgctc ttggcccaga
     1621 ttgacgccat tcgcatgtgc gtaaagagtt atcttacccg gatggcgctc caagtacttt
     1681 tgggccactt ccatgctctt atcggttgag ccgtcatccc aaagtataag ttcccaatcg
     1741 gtgtaagttt gggccaacac cgactcgatt gcgttagcta cgaatttttc gtgattataa
     1801 agtgccgtta aaatagagac gcgaggcata ttcaacgagt cacttcggct ggccgtcgcc
     1861 ccaacgacta aattcttgtt ccatggtttt atcgcaaata cgattgcgtt taacgatttc
     1921 gtcggtgtcc cgcatgcggt cagcgatact ccgaatcatc acgagcaacc aactcgggag
     1981 ttttttcatt tctcgctcca agtgggcctg attaagcgtt aaaattttcg catcagtcgc
     2041 acactcggcc gacgcagaac gcggtttgcc gtcaaaaaat gaaagttctc caacaaattg
     2101 cccagcgtga accagcgcaa ttggtaactt cctaccgtca cattctttaa atatttttac
     2161 gacccctgtt tgaacaatat agagatcttt tgagttctca ccttcatgaa aaagaacatc
     2221 gcccttcttg tactcgacaa gtttttcgtc caatgctctc tcctcgcgta gaactacttt
     2281 ttcttcttac cctcgtcctg aattttaacc tggctcaacg cataatcaac cgttcgttct
     2341 tcccgtaaac gaaattttag ctgacgcaat gagtcttcgt gagattcata catcttccga
     2401 atttcttcgg gcgaagactt catcggttcg gccatttttg cgaattcgac atccaagtca
     2461 gcatctgtaa ctttaatatc ctgctgttcg gcgatttttt gaagtatcaa tgcagcttta
     2521 acttgctcgc cggctcgtgt ttcaagctct ttggaatttt tttccaaata gtcccgaatc
     2581 attttttcgg tgaaaccttg gcgtttaaga acattaaagt tctcgcgcat taggtgttct
     2641 gactgagttc gaaccaacgc agaaggtact tcgaaggtat ttgcatcaat caacgccttg
     2701 agcagatttt ctttgatcgc ctcttttgag ttttttgcgc gttcctcttc taggctctca
     2761 accacttttt tccggagctc taaagcagtt tcacacttaa acttcttagc gaactcatca
     2821 tccaacacgg gaacaacctt cattttaaat tcatga
//
LOCUS       DAOMXO010000286         2833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20157_length_2833_cov_5.210223, whole genome shotgun sequence.
ACCESSION   DAOMXO010000286 DAOMXO010000000
VERSION     DAOMXO010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20157_length_2833_cov_5.210223"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLH57_06720"
     CDS             <1..541
                     /locus_tag="PLH57_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRK02344.1"
                     /translation="QDAITRDNVTVKVNAVVYFRVIDPNRAVVEVENYLMATSKLAQT
                     TLRSVMGQVELDDLLASRDEINQKLQGILDKQTEPWGVKVANVEVKQIDLPVEMQRAM
                     ARQAEAERERRAKVIAADGEFEASKKLGEAAEVMERHPMTLQLRYLQTLSEMSSEKAT
                     HTIIPFPLEFLKSFMGSMK"
     gene            939..2054
                     /locus_tag="PLH57_06725"
     CDS             939..2054
                     /locus_tag="PLH57_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRK02345.1"
                     /translation="MVERENKNILIEGFDLVFREGVQGKSVAHVDRRAAVPLSCDANG
                     KLELRTSLSKKIKITPPLRVQSLGGFIRIHGRQYRDDLYVYGHRGDCVVVNHVDLEKY
                     VAGLLNSEMSVSWNLNALKAQAVAARTYALYQMSLGNPADPTRSPFDLESTVRDQVYE
                     GAHKERYKALKAVQETRGQILTFKNQPVKAFYHSTCGGETENADRVWGARYPYLRPVK
                     CGYCNNSPRFQWTYEVSQSELEKKLLAKNLLKGSLKGIRVIERNSIGRAAFVEVIGSG
                     GKLKVKGTELRDALGSVHLRSTDFRVTASLRSNSENKTIRPSMRLGNWVYSFRGSGSG
                     HGVGMCQYGAKGQGDAGKSYAQILRHYYPLTSIKKIY"
     gene            2090..>2833
                     /locus_tag="PLH57_06730"
     CDS             2090..>2833
                     /locus_tag="PLH57_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRK02346.1"
                     /translation="MASLEDFNFHLPEERIAREPARPRDHSKLLLCSARGRLGEFSGP
                     IAYSERRFHEVLNELNSNDVLVLNNAKVLPVRLLGHRLNDDGTMGGQVEAVLSTPISD
                     LEWNAILHLSARVRPGLKLRFEPGLIAEVLSTHEERLQNAGEVRLRLSGPGLAEQSLE
                     AWLVRHGHVPLPTYMERSDSQQDRTDYQTVYAERIGSAAAPTAGMHFTQPLLDALAEK
                     GVHIERLTLHVGIGTFRPIKATRIEDHQMH"
BASE COUNT          740 a          685 c          723 g          685 t
ORIGIN      
        1 ccaggatgca atcacccgcg acaacgtaac cgtaaaagta aacgcagtag tttattttcg
       61 cgtgatcgac ccgaaccgcg cagtagtaga ggtcgaaaat tacctcatgg ctacctcgaa
      121 gcttgctcaa accacattgc gctcggtcat gggccaagtg gaacttgacg atctgcttgc
      181 gtcgcgcgac gaaatcaatc aaaagctcca gggaattttg gataaacaaa ccgaaccatg
      241 gggcgtgaaa gtcgcgaatg tcgaagttaa gcagatcgat ctacctgtcg aaatgcagcg
      301 tgcaatggct cgccaggctg aagccgagcg tgaacgtcgc gctaaggtga ttgctgctga
      361 tggcgaattc gaagcttcaa aaaaacttgg cgaagccgcc gaagtcatgg agcgtcaccc
      421 gatgaccttg caactgcgct atctgcagac tctttcggaa atgtcgtcgg agaaagccac
      481 tcacacgatt attccgttcc cactcgaatt tttgaaaagt ttcatgggct cgatgaagta
      541 aaaaagaaag gtcccggctt ttgcgcggaa gcgttcgcgc gatagggttt ttttgcctag
      601 tcaaaccggg gcgtctcgtc ctgtagccta gcgccccagt gaagggcgcc cgatttttcc
      661 gggcaaattc gccgggacct ttcatgtccc atttacaaat accggatcgg ttttgtcttc
      721 taaatgcttt acaaaataaa tcaggttaaa ttatccagat ttaacgcctg gcgtcttgct
      781 cgaactggca cctagaccgc tacaatcaaa ggtggaggtt actcacggtg gagtatctca
      841 agcccaagga tgggcaactt ccctttgcca ttcttgtttt ctgtttcctt acaaacttaa
      901 cagttctcca cgcctcgccg gtaccggtgg tgcgtgttat ggttgaacgc gaaaataaaa
      961 atattttaat tgaaggtttc gatcttgttt ttcgcgaagg ggtgcaaggt aaatcggtgg
     1021 cacatgtcga ccgtcgtgca gctgttccac tttcctgcga tgccaacgga aaactcgagt
     1081 tgcgaacctc attgtcaaaa aaaataaaaa tcaccccgcc actacgggtg caaagtttag
     1141 gtggttttat tcgtattcac gggcgtcagt accgcgacga cctctacgtg tatggccatc
     1201 ggggtgactg cgtcgtagtg aatcacgtcg atctcgaaaa gtatgttgcc ggtcttttga
     1261 atagcgagat gtcggtgagt tggaatttaa acgcactaaa ggcgcaagcc gtcgccgcgc
     1321 gaacctacgc actctaccaa atgagcttgg gtaatccggc agatccgacc cgatcaccgt
     1381 ttgacttaga gtcaaccgtt cgtgatcaag tttacgaagg cgctcacaaa gaacgttata
     1441 aagcactcaa agcagtgcaa gaaacacgag ggcagattct gacttttaag aatcaacccg
     1501 ttaaagcgtt ttatcattcg acttgcggcg gcgagactga aaatgcggat cgtgtttggg
     1561 gagcgcgtta tccttatttg aggccagtga agtgcggtta ctgcaataat tctccacgat
     1621 ttcaatggac ctacgaagtg tctcaaagcg aattagaaaa aaagctgctt gccaaaaatt
     1681 tattaaaggg ttcgctcaag ggcattcgag tcatcgaacg caacagtatt gggcgggcgg
     1741 cattcgtcga agtaatagga tcgggtggca aactaaaagt aaaaggtacc gaactgcgag
     1801 atgctcttgg cagtgtgcat ttgcgtagta cggattttcg cgtaactgcg tcgctacgaa
     1861 gcaattcaga aaataaaacc attcgaccga gcatgcgact gggtaactgg gtgtactcgt
     1921 ttcgagggtc agggtctggc cacggcgtag gaatgtgcca atacggcgca aagggccagg
     1981 gggatgctgg taaatcctat gctcaaatac tgcgccacta ttatcccctc acaagtatta
     2041 agaaaattta ttaacgagcg ttcgaaatac cgcgtgctat aaggccacta tggcgtcgct
     2101 cgaagacttt aattttcatt tacccgaaga acgaatcgct cgcgaaccgg cccgccctcg
     2161 cgatcattcg aaactgctat tgtgtagtgc tcgcgggcgt ttgggcgaat tttccggtcc
     2221 tattgcgtat agtgaacggc gattccatga agttctaaat gaactcaatt ccaacgatgt
     2281 gttggtctta aacaacgcca aagtattacc cgtgcgattg cttggccatc gcctgaacga
     2341 cgacggcacg atgggcggcc aagttgaagc ggtattgagt accccgattt ctgatttaga
     2401 atggaacgca attttgcacc tttcggctcg ggtgcgccct ggcttaaagt tacggttcga
     2461 gcccggacta atagcagaag ttttgagtac ccacgaggag cgactacaga acgccggaga
     2521 agtgcgattg cgattgagcg gtcctggact cgcagaacag agtctcgaag catggctcgt
     2581 tcgccacggt cacgttcctc ttcctaccta catggaacgt agcgattctc agcaagaccg
     2641 aacggattat caaactgttt atgccgaacg catcggctca gccgctgccc caacggcggg
     2701 catgcacttt acgcagcctt tgttagacgc gcttgctgaa aaaggcgtgc atatagaacg
     2761 tctcacgctg catgtgggca ttggtacgtt tcgccccata aaagctacca gaatagaaga
     2821 ccatcaaatg cac
//
LOCUS       DAOMXO010000287         2826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20240_length_2826_cov_4.634789, whole genome shotgun sequence.
ACCESSION   DAOMXO010000287 DAOMXO010000000
VERSION     DAOMXO010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20240_length_2826_cov_4.634789"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLH57_06735"
     CDS             <1..186
                     /locus_tag="PLH57_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02347.1"
                     /translation="FVLFPDPWPKARHHERRLNYFRSSSLCFTPVKQRLRRSPSPRWY
                     RLARKWPLRFLRFWVTC"
     gene            147..782
                     /locus_tag="PLH57_06740"
     CDS             147..782
                     /locus_tag="PLH57_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRK02348.1"
                     /translation="MASPFSSILGNVLSSVAGVLTGVLGLFVIPLLAFYLLRDFPKLG
                     GRLMRIIPRNKQRMVQDLRDRLDLVLGGFLRGQLLVSGLLSTYYVFAFLVLGIDLALV
                     LGVLAGVLNLVPYVGIMIVIAMTTLLALLHGATAATFIALGIVFAIGMAVEGVFLTPK
                     IVGNRVGLRPLSILIALMCGGELFGFVGMLLAIPTAATVKVFIEYWIEQRV"
     gene            complement(816..1169)
                     /locus_tag="PLH57_06745"
     CDS             complement(816..1169)
                     /locus_tag="PLH57_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02349.1"
                     /translation="MSQILIRTGVSFSILVPVLILTLFTHTAQAGGTREGRGSRSGGG
                     NCESLVQPNEEFRAGAQSERGLPVSTRRSIPRDRMESVSDQGRTFDEMGNRPHATWDT
                     GLWDTTGGAGGNEAP"
     gene            1333..>2826
                     /locus_tag="PLH57_06750"
     CDS             1333..>2826
                     /locus_tag="PLH57_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02350.1"
                     /translation="MIRWGLAVVLGLSGLATAGSGQATESYYSVPIKSKKTITDDGCV
                     LQIRLFPDDFYFWLTVTRRCGDELPDLVSERSFTSENLATQAYERESQKLDQSHGAST
                     HYQFAPGQTPAEPPKVPNLTAPQDAAAQATPLWVVQNQWNLEWEQRYAQWVTSEVDNG
                     FFLEHGIATDCADVVYAVRWIFARQFGLPVKATIDGDVAFTQNSKKAEWASLPTAEHW
                     AEDQRFRAALEYLMKMTYTRTLPDDTVPVAINEANLKPGIIYLTLSSVSGHAQLLSRV
                     DPKETPFLRFVSSTVPREVRELDGHGFWVSRQPTSDEGFRRFVWPHLTKKSEMPGYST
                     EQYKLSFLRKYLSFSQAVFDRLGVKFDLVRRFSEGTLSMEQLLTERSSIVVQAQQVCG
                     REGACPPGSRDEDSYSTHSRDRRMCELLQHIDLIASECQFRGLDQCDKDRQRRIEVWT
                     MNNGEQDMSVGVVTSVLRAGLIDSEATSSISARWGTDVPALVAKIVSR"
BASE COUNT          631 a          741 c          769 g          685 t
ORIGIN      
        1 ttcgtcctct tccccgaccc ctggcccaag gcccgccacc acgaacgccg cctgaactat
       61 ttccgcagtt cgagtctttg cttcacccca gtaaaacagc gcctacggcg gagtccgtcg
      121 ccccgctggt acaggctggc aaggaaatgg cctctccgtt ttcttcgatt ctgggtaacg
      181 tgttgagttc ggtcgcagga gtgctcaccg gagtgcttgg gcttttcgtg attccgttgc
      241 tcgcatttta tctgttgcgc gactttccga aattaggcgg gcgattgatg agaatcattc
      301 cccgcaacaa gcaacgcatg gtgcaggacc tccgagaccg cctagacctt gtcctcggtg
      361 ggtttctaag ggggcagctt ttggtgagtg ggctactctc gacttactac gtttttgcct
      421 ttctcgtgct gggcatagat ctcgcgctgg tccttggtgt actggcgggc gtgctcaatt
      481 tagtgcccta cgtaggaatc atgatcgtca ttgcaatgac aacgctacta gctctattgc
      541 acggagcgac ggctgcaact tttattgcgc tcggaatcgt attcgccatc ggcatggcgg
      601 tcgaaggcgt gtttctgacc cccaaaatcg tagggaaccg agttggcctc cgacctctca
      661 gcatattgat tgcgctcatg tgcgggggcg agcttttcgg attcgtagga atgttgcttg
      721 cgattcccac cgccgccacc gttaaagtgt ttattgaata ctggatcgaa cagcgggtgt
      781 aacctcgcgt agcgattgaa acacccgctt tttagttagg gcgcttcgtt gccgccggcg
      841 ccaccggtcg tgtcccagag gccagtatcc caggttgcat gtgggcggtt gcccatttca
      901 tcgaaagtgc gaccttgatc tgagacagat tccatccggt cgcgcggtat agagcgacgg
      961 gtagaaacgg gcaaaccgcg ttcgctctgc gctccagcgc gaaactcttc gtttggttgt
     1021 accaaggatt cgcaattacc tccgcccgat ctcgagccgc ggccttcgcg ggtaccacca
     1081 gcctgcgcgg tgtgggtgaa taaagtcaaa ataagaacag gaacaagtat agaaaaagag
     1141 accccggtcc gaatcaaaat ctgactcatg ttttactacc aacccttcgc ccattctcgt
     1201 acctgtcaag caaaatagag tcaacccggg ttgctttttt tcttgaaaaa ctagtttggg
     1261 ccgattgccg actccagaaa aatgcggtca agttttgttg ggaaacaaaa acaaagggga
     1321 aacgtcatgc gaatgattcg ctgggggttg gcagttgttt tgggactttc ggggttggcc
     1381 acggcgggta gcggccaagc gacggagagc tactactcgg taccaataaa atccaaaaaa
     1441 accatcactg acgacggttg tgtactccag atacgccttt ttcccgacga cttttatttt
     1501 tggttaacgg tcaccaggcg ttgcggagac gaactcccag atctagtttc tgaaagaagt
     1561 tttacctcgg aaaatctggc gacccaggcg tatgaacgag aaagtcaaaa actcgatcag
     1621 tcccatggcg cttcgaccca ctatcaattt gcgcccggcc aaacgccagc tgagccccct
     1681 aaggttccga acctcacggc tccccaagac gctgccgcgc aggcgacgcc actgtgggtg
     1741 gtgcaaaatc agtggaacct agagtgggag cagcggtacg cccagtgggt gacctccgaa
     1801 gtggataatg ggttttttct agagcacgga attgctacgg actgcgccga cgtggtttac
     1861 gctgttcgct ggatatttgc ccggcaattc ggattgccag ttaaagctac gatcgacggt
     1921 gacgtcgcgt tcactcaaaa ttccaaaaaa gcagagtggg caagcctccc cacggccgag
     1981 cattgggcgg aggatcaacg gtttagagca gcattagaat acctcatgaa aatgacctac
     2041 acgcgaacgc tccctgacga caccgttcca gtggccatca atgaggcgaa tttaaagccc
     2101 ggcattatct acttaactct ttcgagcgta tccggtcatg cccagctcct gagtcgggtt
     2161 gatccaaagg aaaccccgtt tcttaggttc gtttcgtcca cggttcctcg cgaagttcgc
     2221 gagttggacg gacacggatt ttgggtcagt cgccagccca catctgatga agggtttcgt
     2281 cgttttgttt ggcctcactt aacgaagaaa tctgagatgc cgggttattc gactgaacaa
     2341 tacaaactat cctttcttcg aaaatatctg agtttttcac aggcggtgtt cgatcgattg
     2401 ggtgtaaagt tcgatctagt gcggcggttc tccgagggta ctctgagtat ggagcagctg
     2461 ttaactgaac gaagctcaat tgttgttcag gcccagcaag tatgcgggcg cgagggcgct
     2521 tgcccccctg gtagccgcga cgaagatagt tactctaccc actcccgtga ccggcgtatg
     2581 tgcgagcttc tgcagcacat tgatttaatc gcaagcgagt gtcagttccg agggctcgat
     2641 cagtgtgaca aagaccgaca acgaaggatc gaggtctgga ccatgaacaa tggagaacag
     2701 gacatgagcg ttggagtcgt aaccagcgtg ctgcgggctg gtttaattga ctcggaagct
     2761 accagttcaa tttcagctcg ttggggcact gacgttcccg cgttggtcgc aaaaattgtg
     2821 agtcgc
//
LOCUS       DAOMXO010000288         2822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20271_length_2822_cov_5.568124, whole genome shotgun sequence.
ACCESSION   DAOMXO010000288 DAOMXO010000000
VERSION     DAOMXO010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20271_length_2822_cov_5.568124"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLH57_06755"
     CDS             complement(<1..413)
                     /locus_tag="PLH57_06755"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRK02351.1"
                     /translation="MSILRGFLQPRHLFWPVAAGICLLLALGRSVSAVSFGAGDTVGA
                     VRQIQVVGNKKIEVDAILAKIKTKAGEPLSRAQVSDDIRALHALGYFDDVAFDWNDGV
                     VTIKVLERPTISKISFDGNDQVSTSDLKETIKVKE"
     gene            complement(471..1148)
                     /locus_tag="PLH57_06760"
     CDS             complement(471..1148)
                     /locus_tag="PLH57_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK02352.1"
                     /translation="MNSEIVLEARGVQKSFKKGKLVIPVLRGVDLAIKRGETVAIVGA
                     SGAGKSTLLHILGTLEAPSGGHVLFQGKNISSQSEERRCEFRNRHIGFIFQFHYLLPE
                     FSALENVMMPGLIAGRHEHQLKAEALALLEEVGLSHRVHHKPGELSGGESQRVAVARA
                     LMMKPDVILGDELTGNLDSQNSEMLLSMLLSINQRHGVSVVVVTHDLKLAASMGRTIT
                     MQDGKLV"
     gene            complement(1141..2352)
                     /locus_tag="PLH57_06765"
     CDS             complement(1141..2352)
                     /locus_tag="PLH57_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK02353.1"
                     /translation="MSLVAKIAFRFLGNRKSSSFAPFVAAISVFGLCLGVTALLVVTS
                     VMNGFERELKRALTAFHGHVLMFSRAEPVPNAQAIANDIKKTFPLVKEVSPYIFAEVM
                     LSAKKGVAGSVVEGVDLKTFDKVSQMPNRLKEGRLPTAGAATVEITLGSELARRIGAQ
                     VGGDVVLTVPFAKEGGEPLAQRLKVVGIMQLGMYEYDNKYALVELSELQKTLSVGDRV
                     NAFKMLTASPEKSILVTEGLNDKYVYPLRAKDWTSLNRNLFYAIQLEKVVIAIILFAI
                     VLVASFNIISTLMMLVHDKKSQISMLKALGWSMRRTFATFLLIGGFMSLLGAIAGFAL
                     AQMLCRLVQWKSIIDLPPEVYFLSRLPVEIRPAEWAIILLSSILLALGATLVPAWKVS
                     RESPVEGLRYE"
     gene            complement(2371..>2822)
                     /locus_tag="PLH57_06770"
     CDS             complement(2371..>2822)
                     /locus_tag="PLH57_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRK02354.1"
                     /translation="MAIFDAEVEKNLIQPTFVTQYPTEVSFLSRKNEKDPSTVDRFEL
                     FIYGREMANAFSELNDPIDQRERFESQASAKAAGDDEACDVDEDFLFALEHGMPPTAG
                     EGLGIDRLVMLLTDNASIRDVIFFPHMRPQAPVKAASNSGTTDSSQP"
BASE COUNT          754 a          749 c          603 g          716 t
ORIGIN      
        1 tattctttaa ccttaatagt ttctttgagg tcactggtgg aaacctggtc gttaccatcg
       61 aaagaaattt ttgaaatcgt cggtcgctcc aatactttga tcgtaacaac tccatcgttc
      121 caatcaaaag caacatcgtc aaagtaccct agcgcgtgaa gggccctaat gtcgtcactc
      181 acctgagcgc gtgaaagtgg ctcacctgct ttggttttaa ttttagcgag gattgcgtcg
      241 acttcgattt ttttatttcc aacgacctga atttgacgaa cggcgccgac agtgtctccc
      301 gcgccgaatg aaacggcgga aacacttcgg ccaagagcca acaacaggca aatgcccgct
      361 gcgactggcc agaaaaggtg gcgaggctgc aaaaaaccgc gaagtatact caagtttaag
      421 aatgctttct tgttaggaga tctatatttt tgggggaatc cttcgtcaac ctaaactaat
      481 ttgccatctt gcatggtaat cgttcgcccc atgcttgcgg caagcttaag atcgtgggtg
      541 acgaccacga cactcacacc atgtctttga ttaatgctta gcagcatcga aagcaacatt
      601 tcactgtttt gactatccaa attaccggtc aactcgtcac ccaagattac atctggtttc
      661 atcattaaag ctcgcgcaac cgctacccgc tggctttctc cgcccgagag ctcacctggt
      721 ttgtggtgca ctcggtgcga caaaccaacc tcttccagga gcgccaaggc ctcagccttt
      781 aattggtgct cgtggcgacc cgcaatcaat cccggcatca ttacattctc gagcgccgag
      841 aactctggaa gtaaataatg aaattgaaaa ataaatccta tgtgtcgatt ccgaaactca
      901 catcgtcgct cttccgactg agaagaaata ttcttaccct gaaacaacac gtgaccgccc
      961 gacggtgcct caagagttcc cagaatatgt aacagggtac tttttcccgc accggacgcc
     1021 cctacgattg caacagtttc ccctctttta attgcgagat cgactcctcg caaaacaggg
     1081 ataacaagtt tgcctttttt gaacgacttt tgaaccccac gagcttcgag cacaatttcg
     1141 ctattcatac cgaagcccct ccaccggact ttctcgacta actttccagg caggcaccaa
     1201 agtcgcgccc aaagccaata agattgaact cagcagaatt atcgcccact ccgcaggtcg
     1261 aatttccacc ggaagtctcg atagaaaata tacttcgggg ggcaaatcga taatcgattt
     1321 ccactgcacc agacggcaca acatttgagc aagcgcaaac ccggcgatgg cacctaataa
     1381 gctcataaat ccaccgatta acaaaaacgt cgcgaaagtt cgtcgcatgc tccaccccaa
     1441 ggctttcagc attgaaattt ggctcttctt gtcatggacc aacatcatga gggtgctaat
     1501 aatattgaag ctcgccacta aaacgatggc aaacaagata attgcaatca ccactttttc
     1561 gagctgaatc gcgtagaata aattgcgatt gagagaggtc cagtcctttg ctcgtagagg
     1621 gtaaacgtat ttgtcgttta atccttcggt gaccaagata cttttttcag gcgatgccgt
     1681 gagcatttta aaggcattta ctcgatcccc aacagacaga gttttctgca actcagataa
     1741 ttccaccagt gcatacttgt tgtcgtactc gtacatacct agctgcatga tgcctacgac
     1801 tttcaagcgt tgtgccaagg gttcgccccc ctctttcgcg aagggcacag tcaatacaac
     1861 gtctcctccc acttgggctc caattcggcg cgcaagttcg ctgcccaggg taatttctac
     1921 agtcgcagcc cccgctgtag gtaatcgccc ttcctttaaa cgattgggca tttggctaac
     1981 tttatcgaag gttttgagat caacaccctc gacaaccgaa ccagctacgc cttttttggc
     2041 gcttaacatg acttccgcaa aaatgtaggg cgacacttct ttaacgagcg ggaaggtttt
     2101 ttttatgtcg tttgcgatcg cctgggcatt tggtaccggc tctgcacgcg agaacataag
     2161 tacgtgcccg tgaaaagcag taagtgcgcg ctttagttcg cgctcaaatc cattcataac
     2221 gctcgtgaca acaaggagcg cagtaacacc aagacaaagc ccaaacaccg aaattgcagc
     2281 tacgaacggc gcaaaactcg acgactttcg atttcctaaa aaccgaaaag caatctttgc
     2341 taccaagctc atttaggagt tctttcctta tcagggttgc gacgaatcag tcgtcccact
     2401 attggatgcc gctttaacgg gcgcttgcgg ccgcatgtgt ggaaaaaata tcacatcgcg
     2461 aatgctggcg ttatcagtaa gtagcattac gagacggtca atacccaagc cctcgcctgc
     2521 agtgggcggc attccgtgct ctaaagcaaa taaaaaatcc tcgtcgacat cgcaggcttc
     2581 gtcatcacct gcggccttgg cactggcttg cgattcaaaa cgctcgcgtt gatcgatagg
     2641 gtcgttcagc tcagaaaacg cattcgccat ctcacgacca taaataaata gttcaaaacg
     2701 atcgaccgtc gatggatctt tttcgttttt tcgcgacaag aaagaaactt cggtaggata
     2761 ctgtgtcacg aatgtcggtt gaattaggtt cttttccact tcagcatcga aaatggccat
     2821 ta
//
LOCUS       DAOMXO010000289         2822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20274_length_2822_cov_4.004337, whole genome shotgun sequence.
ACCESSION   DAOMXO010000289 DAOMXO010000000
VERSION     DAOMXO010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20274_length_2822_cov_4.004337"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1919)
                     /gene="rnr"
                     /locus_tag="PLH57_06775"
     CDS             complement(<1..1919)
                     /gene="rnr"
                     /locus_tag="PLH57_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRK02355.1"
                     /translation="MKKSKRSSRRGERRARSHEAGKKKPTAGFSGRPPEKKRKSLAEQ
                     ARERNERHRAGKKTGSQNHHPPGKNLVILQGAVQKNRKGFGFLLQEKEDVFIPPELAA
                     NLLTGDKIRMRYNPRSREAIGVELVERGLKQFIGTYHAGFSNRFVELVDRTMREEVNI
                     PHPPAIVGLRHGDKVLVEVTKYRPKLEGRIVRSFGPKLAPKFDTLAVVVRSNGPQEFS
                     QEASSQAERLSVEIQERENIAGYPGRKDLREKHFVTIDGKDARDFDDAVLVEKTDSGY
                     VLYVAIADVGEFVRPGDILDEEAYARATSVYFPEWVIPMLPEALSNGACSLRPNEEKL
                     TLTCEMHFDLNGKKRSERVYESVIRSHRRCIYEDVQKEYDSGDKFWAVPYELYRILRT
                     KRIDRGALDLDLPEAKIILDENSDTIDIQKTERLDSHRLIEEFMIAANEAVSELMERD
                     QWPFVYRVHEPPQAEALERFDRFASALGLKPKLGDGASPKLFSNFLSSIKENPYLETL
                     SYLMLRSLKQARYEPSNLKHFGLASRAYSHFTSPIRRYPDLMVHRILKRYVRRKPFHG
                     GERNDFEAYLDDACLHCSTQERKAQDLDRTVTKIKKARFMEKHLGKEFEARVYNVGEN
                     GLYVELKQWFIEGLVP"
     gene            complement(1960..2043)
                     /locus_tag="PLH57_06780"
     tRNA            complement(1960..2043)
                     /locus_tag="PLH57_06780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2007..2009),aa:Leu,seq:cag)
     gene            complement(2133..>2822)
                     /gene="rmuC"
                     /locus_tag="PLH57_06785"
     CDS             complement(2133..>2822)
                     /gene="rmuC"
                     /locus_tag="PLH57_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRK02356.1"
                     /translation="IGEGNLERLLTDFLPAGFFAMQHPIQGSLVDAVIKFPHLNLVLP
                     IDSKFSLEQVSALFEAQSTPEDLKAARKRLADVSKQNAKDIKNKYIKPEHGTTDYALM
                     FLPSETLYFEVIRDNELWTSLVDLKVFPVSPNTLAITINSIGKSLHYYEMARGVQRTV
                     SQIQQAQTHLGNFKKRFDDVGEKLSKAQDSFQKAGTHLSYYSSSIERLTGDVGTLPEA
                     APEFKTPSVPQ"
BASE COUNT          695 a          694 c          650 g          783 t
ORIGIN      
        1 ggaacgagcc cttcaataaa ccactgcttt agctcgacgt acaaaccgtt ctcacccacg
       61 ttgtaaactc gtgcttcaaa ttccttaccc aagtgttttt ccatgaatcg agcttttttt
      121 attttggtaa ctgtacgatc aaggtcttgg gcttttcgtt cttgtgtcga acagtgtaaa
      181 caagcgtcgt cgagatacgc ctcaaagtcg ttgcgttcac caccgtgaaa aggtttacgt
      241 cgcacataac gttttaaaat acggtggacc attaaatccg gatagcgacg aatcggcgaa
      301 gtaaaatggg aatacgccct cgaagcaagt ccgaaatgtt ttagatttga aggttcataa
      361 cgcgcctgct tgagtgacct caacattaga taactaagag tctctaaata agggttttct
      421 ttgatgctgg atagaaaatt ggaaaatagt tttgggctcg caccgtcgcc gagctttggc
      481 tttaaaccaa gagccgacgc gaatcgatcg aaccgttcca acgcctcagc ctgcggcggt
      541 tcatggactc ggtaaacaaa cggccactgg tcccgctcca ttaattcgct aacggcctcg
      601 ttagcagcga tcataaactc ttcaatcaag cggtgggaat caagccgttc agttttttga
      661 atatcaatag tgtcgctgtt ttcatcgagt attattttcg cttctggcaa atcaaggtcc
      721 aaggcgccgc gatcgattcg tttcgtacga agaatccgat ataactcgta cggcactgcc
      781 cagaatttat cgccactgtc gtattccttc tgtacgtctt cataaataca acggcggtgc
      841 gaacgaatca cagattcata aactcgctca gaccttttct ttccatttaa atcgaaatgc
      901 atttcacagg tgagtgtgag tttttcttcg ttgggtcgca aactgcaggc accattactg
      961 agggcctccg gcaacattgg tatgacccac tctggaaaat acacacttgt ggcccgcgca
     1021 taggcttctt cgtctaaaat atcacctggt cgtacaaact ctcccacgtc ggcaatagct
     1081 acgtagagga cataacctga atctgttttt tctactaaaa ctgcgtcgtc aaaatcgcgt
     1141 gcgtcttttc catcaattgt gacaaagtgc ttttctctca aatcttttcg accggggtaa
     1201 cccgcgatat tttcgcgttc ttgaatttca acacttaatc tttcggcttg gctggaagct
     1261 tcttgcgaaa actcctgcgg cccatttgaa cgaacgacca cagctaaagt gtcgaacttt
     1321 ggcgccagct ttgggccgaa tgaacggaca atgcgcccct ccagcttcgg acggtattta
     1381 gtgacttcga ccaacacttt gtctccgtgg cgcaaaccga cgattgcggg ggggtgagga
     1441 atgttcactt cttctcgcat agtgcgatcc accaactcca cgaaccgatt cgaaaaaccc
     1501 gcgtgatagg tacctataaa ttgctttaat cctcgttcaa ctaactctac gccgattgct
     1561 tcacgagagc gcggattgta gcgcatgcgg attttgtcgc ccgtaagcaa attcgctgca
     1621 agctccggcg gaatgaagac atcttctttt tcttgaagta gaaaaccgaa tcctttgcga
     1681 ttcttctgaa cggcgccctg gaggatgacc aaatttttcc cgggagggtg atgattctga
     1741 gaaccggttt tttttcctgc gcggtgtcgt tcgttgcgct cacgcgcttg ttccgctagc
     1801 gacttccgtt ttttttccgg tggacgaccc gaaaacccgg cggttggttt ttttttgccc
     1861 gcttcatggg atcgagcgcg acgttcgcca cgtcttgagc tgcgttttga tttcttcatg
     1921 gcttacatct ttgcttacga atagggggat ggcaatgggt ggtgcggacg gagagactcg
     1981 aactctcatg cttttggcgt acgaccctga atcgtatgcg tctaccaatt ccgccacgtc
     2041 cgcataagga cttttaagac gacttaaacc ctctgctaac aatgccacga gcggttgtac
     2101 agaaaaaata gctctgcctt tgtcctaaac atctactgtg gtaccgaggg ggttttaaat
     2161 tcaggcgcag cctcgggcaa ggtgccgacg tcgccggtca agcgttcgat cgaagacgaa
     2221 taataggaaa ggtgggttcc cgccttctga aaggaatctt gcgctttcga gagtttctca
     2281 cccacatcgt caaatcgttt cttaaagtta cccaagtgtg tttgagcctg ctgaatttgt
     2341 gataccgtac gctgaacacc tcgcgccatt tcatagtagt gaagtgattt accgatcgaa
     2401 ttaatggtaa tcgccagggt attgggcgaa accggaaaca ccttgagatc caccaaagaa
     2461 gtccaaagct cgttgtcacg tatcacttcg aaatacagag tttccgaagg cagaaacatt
     2521 aaggcataat ccgttgttcc atgttcaggt ttaatatatt tatttttgat gtccttcgca
     2581 ttttgtttcg atacatctgc aaggcgtttg cgcgcagctt ttaggtcttc cggggtagat
     2641 tgcgcctcaa atagtgccga cacttgttcg agcgaaaact tactgtcgat cggcaaaacg
     2701 aggttgagat ggggaaactt gataactgca tcaaccaaag agccctgaat ggggtgctgc
     2761 atcgcaaaaa atccggccgg taaaaagtcc gttaacagcc tctctaaatt gccttcgcca
     2821 at
//
LOCUS       DAOMXO010000290         2820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20289_length_2820_cov_4.664376, whole genome shotgun sequence.
ACCESSION   DAOMXO010000290 DAOMXO010000000
VERSION     DAOMXO010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20289_length_2820_cov_4.664376"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1594)
                     /locus_tag="PLH57_06790"
     CDS             complement(104..1594)
                     /locus_tag="PLH57_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK02357.1"
                     /translation="MPPIRFGHFGLAYICALVLFAAPAEAAKKNKSPANAPRNSIGVS
                     VRLAFEAESLDWTRGDVPIHLINNVVEGLYEISADGRVVPAQALKLPKQIGPSRWEIR
                     LKPRLKWSDGLALTAQDYVYAWNRLRSKELASSYSALLDDVASYRASDANTLEIITKK
                     NVALEPALFTHWCTFPVSSSHLELHGEKWTKNAATIPVNGPYRITSGNLGTGFTLAPN
                     SHHRSPGTLKKIEFLVIADDSTALRLYETGRLHFMTDLAGVDRLKIRSHADYRSVKAP
                     VLIYLAMDLGHSLFSVTSNRRIIAEAIDRRSIERVLDGLATPSKNLSPDQPITETDAA
                     YSLPSTGTLAGARFDFAFYEKGSNRNLVELIQNEFKKKLHIDATLITHDVKTYWAKLK
                     ERPFPLFLNSYGPPIWSSSYYFKLLTSSHPQNLGRWSNLEYDKAVEQSNWRKAALIFS
                     REQPIIPLYFRRFEYLQKPELKNVILNPMTSLYLKKATLDAPFKAR"
     gene            1941..2768
                     /locus_tag="PLH57_06795"
     CDS             1941..2768
                     /locus_tag="PLH57_06795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK02358.1"
                     /translation="MKKTILLVIPCFNPSAAGWRGLSQYLERLTGLALRTAPHLEFQA
                     IVVDDGSTLWCEPPELEKDRPYQLVRLEKNLGKGGAIGRILELSHDRYDLLAITDFDT
                     PYSERDLLGMCCLVEWNAVQVCIGDRSMDSSYETSVGPGRLLFHRAGRLLVRTMIAGG
                     FSDTQCGIKVYSYPFVKKIAAVARIRGFLFDLEWVYIALKHGLTVARWPVAISEKHQS
                     GKWFSKRYFKCFFELMHIIVGILSSKYKTSELQILLKKARLSHTGLGDHSKNAEFYC"
BASE COUNT          724 a          599 c          751 g          746 t
ORIGIN      
        1 gattatcttg aaaaactact agctcgtaaa agcgtagaaa ctcgttccga gtgggtcttt
       61 tggccgggta gcgagccagc cccgctctcg gaagggttct acctcaccgt gctttgaatg
      121 gggcatcgag cgtggctttt ttcagataaa gcgatgtcat cggatttaga attacgtttt
      181 tgagttcagg tttctgtaaa tattcgaagc gtcgaaaata aagtggtatg atcggctgtt
      241 cacgtgaaaa aattaaggcg gcttttcgcc aattgctctg ttcgaccgct ttgtcgtatt
      301 cgaggttgga ccacctaccg agattttggg gatgcgagct ggtaagaagc ttgaaatagt
      361 aactcgagct ccagatcgga gggccatatg aattcaaaaa taatgggaag ggtcgttcct
      421 tcagtttggc ccagtaggtt ttaacatcgt gggtaatcaa ggtcgcgtcg atgtgtagct
      481 tttttttaaa ctcattctga ataagttcaa ctaaattgcg attcgagcct ttttcgtaaa
      541 acgcaaaatc aaaacgtgcg ccggcaaggg tgccggtaga cggtaatgaa taagcggcgt
      601 cagtttccgt gatcggctgg tccggactta aatttttcga aggtgtggcg agaccgtcta
      661 acacccgttc gattgatcta cgatcaatgg cttcagctat tattctacgg ttactggtta
      721 cggaaaatag agaatgaccg agatccatcg ccaaataaat gagtacgggc gcttttacag
      781 agcggtagtc agcatgagac cgaattttga gtcgatcaac gcctgcaagg tcggtcatga
      841 aatgaaggcg tccagtttca taaaggcgaa gcgcggtcga gtcgtctgct ataaccagaa
      901 attctatttt ttttagtgtc cctggcgatc ggtgatgaga attaggcgcc aacgtgaatc
      961 cagtaccgag gttgccagaa gtaatgcgat aggggccgtt cacgggtatc gtagcagcgt
     1021 ttttggtcca tttttcgcca tgaagttcaa ggtgcgacga ggaaaccggg aaagtacacc
     1081 aatgagtgaa gagggctggt tctagagcga cattcttttt ggttatgatt tcgagtgtgt
     1141 tcgcgtcgga agctcggtac gaagcaacgt catcgagcaa agcgctgtag cttgatgcga
     1201 gctccttgct tcgcaaacga ttccaagcat agacatagtc ttgcgcagtc aaggcgagtc
     1261 cgtcggacca tttgagtcgc ggcttaagtc taatttccca tcgtgaaggt ccgatttgtt
     1321 ttggcagttt tagcgcttga gcgggtacga ctcttccgtc tgcggaaatt tcatacagcc
     1381 cctccaccac gttattgatc agatgaatcg gcacatcgcc tcgcgtccaa tcgagcgact
     1441 cggcttcgaa agccagacgg acggaaaccc cgatcgagtt ccgtggagca ttcgcgggag
     1501 atttattctt tttagcagcc tcggcgggcg cagcaaaaag aacgagtgca caaatgtagg
     1561 caaggccaaa gtgaccaaag cggatggggg gcattttgct cctagcttcg aaaatggtgt
     1621 cgattcggca ccaactcagt gtagcaaaat caacgagtta gatccaacgc tttggtggca
     1681 cggaccttga agtatggtct gattcgagat ggcgccgcga gggcaatgaa agttgaattc
     1741 gcgggaactt agagcagttg aagcagtacg tagtcagggg ttgtcggtcg gtcatcttcc
     1801 tttccataaa tacttcagaa aaatgaaaaa cctaccgtgc accgctggtt ccccgaaagg
     1861 gggccagccg gcattacttt ttaagtcagg ttggtcgcta gattgacatc aatgcacgcg
     1921 tttcatagcg tcggtgggaa atgaaaaaga ctattttgct tgtgatccct tgctttaatc
     1981 ctagcgctgc agggtggcgg gggctcagtc aatatctcga gcgattaaca gggctcgcgc
     2041 tacgaacggc gccgcactta gaatttcaag ctattgtagt agacgatgga tcaacgcttt
     2101 ggtgtgagcc cccggagtta gaaaaagatc gcccgtacca gctggtgcga ctcgaaaaaa
     2161 atctaggcaa aggtggggcc attgggcgaa ttttagagtt gagtcatgat cgatatgact
     2221 tattggctat taccgatttc gacaccccct acagtgaacg tgacctactg gggatgtgct
     2281 gtttggtaga gtggaacgca gttcaagttt gcatcggtga tcgctcaatg gacagttcct
     2341 atgaaacttc tgtggggccc ggtcgtttac tatttcacag ggctggtcgt cttctcgttc
     2401 gcaccatgat tgcaggtggg ttcagtgaca cccagtgtgg tatcaaagtt tactcctatc
     2461 cgtttgttaa aaaaatcgca gcagtggcca gaatccgagg attccttttt gacttggaat
     2521 gggtctatat cgcactcaaa cacggattga cggttgcgag atggcccgtt gcgatttcgg
     2581 aaaaacacca gtctggaaaa tggttttcaa aaaggtattt taagtgtttt ttcgaactta
     2641 tgcacattat tgtaggaata ttaagttcga agtacaaaac ctctgaacta cagattttgc
     2701 taaagaaagc tcgacttagt cacactggtc ttggagacca ttcaaaaaat gcagaattct
     2761 attgttagga aagaaaaaga ccttgcggag attccgggaa actatcagcg aacggctctc
//
LOCUS       DAOMXO010000291         2817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20315_length_2817_cov_4.854091, whole genome shotgun sequence.
ACCESSION   DAOMXO010000291 DAOMXO010000000
VERSION     DAOMXO010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20315_length_2817_cov_4.854091"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLH57_06800"
     CDS             complement(<1..346)
                     /locus_tag="PLH57_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRK02359.1"
                     /translation="MISRQSEEVLNRAGRYAFEKRHEYCTLEHVLWSLLQEDDIVEII
                     EACAVNSDDLRQELEKHLDTEVPKASLVKRESGDEVPEPPVATLAIQRMIQRAVFHIQ
                     SAGKDEVQPKDLL"
     gene            complement(375..713)
                     /gene="clpS"
                     /locus_tag="PLH57_06805"
     CDS             complement(375..713)
                     /gene="clpS"
                     /locus_tag="PLH57_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRK02360.1"
                     /translation="MSGGGAGQSGQDDSQSDVDLELERPDVSSPGKWAVVLHNDDYTT
                     MEFVIEVLQRFFKKSDQEAAEIMLKVHHEGRGIAGIYSREIAETKVLQVSDYAKAHGH
                     PLRCTAEPTS"
     gene            complement(716..1624)
                     /locus_tag="PLH57_06810"
     CDS             complement(716..1624)
                     /locus_tag="PLH57_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRK02361.1"
                     /translation="MRIGIDLGGTKIEVLVLDRSGAELFRKRAATPQGDYEGTIREMV
                     ALVTGAELATGVRNCTIGVAIPGAESSKTGLIKNSNSLCLIGKPFSKDLADAFGRPVR
                     LANDANCFALSETTDGAAKNRKLVFGVILGTGVGGGLVVNGQVIAGANSIAGEWGHNP
                     LPWMAINEYPGPECYCGKRGCIETFLSGKALERMYEEATRKSLLAKEITKLAEKGDAT
                     AGKLLEHYEDRLARALIHLINILDPETIVLGGGMSNEDRLYRNVPERWKKYMFSDALD
                     TELVKAKHGDSSGVRGAAWLWPEEGV"
     gene            complement(1698..2354)
                     /locus_tag="PLH57_06815"
     CDS             complement(1698..2354)
                     /locus_tag="PLH57_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02362.1"
                     /translation="MNNTRLFGRVGMTLSSVLVLLHIATLAQAKVKAPKDCPDKDKAI
                     PIGVPIDFCRVAHLTPMLLQRASEPGFGSGSLLAQIGNNRGYQYYFKRFFVITDYEKD
                     PGKVIVDRTFVVKDLKKWVPIYAKQGRNIVELRAKKINVKGGIVTVRFYTNVLSGSHK
                     DFDLKITFSPNNEPEMFIQDKVFNSLEITPSRLFNKPVGIGILRFEYYGAEALTLNLV
                     "
     gene            2470..>2817
                     /locus_tag="PLH57_06820"
     CDS             2470..>2817
                     /locus_tag="PLH57_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02363.1"
                     /translation="MGWFSFALGVFFFVPALSFAENFDEISAEIVSPTLIASEIFHSW
                     EYQIPSGYRIKTHGTDSTTEGQRKSPTNAALTRKTQSEVLPIFTELMKEVQLIRTDQQ
                     KENFLRGALYLAKK"
BASE COUNT          663 a          744 c          636 g          774 t
ORIGIN      
        1 ttaaaagatc tttaggttga acttcgtctt tacccgctga ctgtatatga aatacggcac
       61 gctggatcat tcgctgaatc gcaagcgtgg cgaccggcgg ttctggaacc tcatctccgc
      121 tctcgcgttt aacgagcgat gcttttggga cttccgtatc aagatgtttt tctaattcct
      181 ggcgcaggtc atcggaattc acagcacacg cttcaataat ttcgacgata tcgtcttctt
      241 gaagcaaact ccaaagtacg tgttccagag tgcaatactc atggcgtttt tcaaaagcat
      301 accgccctgc gcggttgagt acttcttcgg attgacgaga gatcattcga ttctccttcc
      361 ttcaaacact ctgttcaact ggtcggttcc gccgtgcatc gaagggggtg cccgtgagct
      421 ttggcgtaat cacttacttg caggactttg gtttccgcga tttcgcgaga atatattccc
      481 gcgatgccgc gcccttcatg gtgaaccttt aacataattt cggcagcttc ttggtcgctc
      541 ttcttaaaaa agcgctgtaa aacttcaatc acgaattcca tggtcgtgta atcgtcgttg
      601 tgtaggacta cggcccattt gccgggcgac gatacgtcag gccgctcgag ctccaggtct
      661 acgtccgact gggaatcgtc ttggccggac tgccccgccc caccaccgct catggctaaa
      721 ccccttcctc gggccaaagc caggctgccc cgcggacccc actcgaatca ccgtgttttg
      781 ctttcactag ctcggtgtcg agcgcgtcag aaaacatata ctttttccat cgctctggaa
      841 cgttgcgata aagccgatcc tcgttcgaca taccgccgcc aagcacaatg gtttcgggat
      901 ccagaatatt gatcaagtgt attaaagcgc gcgccaatct atcttcgtag tgttcaagca
      961 gttttccggc cgtcgcatca cccttttccg ccaattttgt gatttcctta gctagcagtg
     1021 attttcgcgt ggcttcttcg tacattcgct ccaatgcttt gccagacagg aaagtttcga
     1081 tacagcctcg ttttccgcaa taacactctg gtcctgggta ttcgttaatg gccatccagg
     1141 gcagtggatt atggccccac tcaccggcga tcgaattcgc tccggcaatc acctgaccat
     1201 ttacgacaag tcccccacca acgccggtcc ccagaatgac tccgaaaact aattttcgat
     1261 ttttcgctgc cccatcggta gtttccgata aagcaaaaca gttcgcgtcg ttcgccaagc
     1321 gcaccgggcg accaaacgcg tctgcgaggt ctttggaaaa aggttttccg attaagcaga
     1381 gcgagttgct gttctttatc agtcccgttt tggatgactc agctccagga atcgctactc
     1441 caattgtgca attccggacg ccggtggcta gttcggctcc cgtcaccaat gccaccattt
     1501 cgcgaatcgt gccttcgtaa tcaccttgcg gagtagcggc ccgtttccga aataattcgg
     1561 ccccgctccg gtccaagact agcacttcga ttttcgtacc gcctaaatcg attcctattc
     1621 gcatgacgct ccattctctg tggtctcgaa gcccgattcc caggcgtcgc tacacaggtg
     1681 aatggattta cgataaacta gacgagattc agcgtcaaag cttcggcccc ataatactcg
     1741 aaccgcagaa tgccaattcc caccggttta ttaaaaagac gcgaaggcgt tatttctaaa
     1801 gagttaaata ccttgtcctg aataaacatt tccggctcgt tgtttgggga aaaagttatt
     1861 ttaagatcga aatctttgtg ggagcctgaa agtacattgg tataaaaacg tacggttacg
     1921 atcccgcctt tgacattaat ttttttggct cgcaactcaa cgatgttacg cccctgcttg
     1981 gcgtaaattg gaacccattt ttttaagtct ttaacgacaa acgtgcggtc gacgattact
     2041 tttccggggt ccttttcgta atcggtaatc acgaagaacc gtttaaagta atactggtag
     2101 ccgcgattgt tgcctatctg ggccagtagt gagcccgaac caaaccccgg ctccgatgca
     2161 cgttggagca gcatgggcgt gagatgcgcg acacgacaaa aatctatcgg taccccaatc
     2221 ggaatggcct tgtctttgtc cggacagtct ttaggcgcct taactttggc ctgcgctaga
     2281 gtagcaatgt gcagaagcac caaaacgctc gaaagtgtca taccgacacg cccgaacaac
     2341 ctcgtgttgt tcataatgtt ccctccatga acggtgaacc gctttgtaga ctcagtttag
     2401 cgcaagttat tattcaaaat attaattttt tgttgttttt gtgtgggatt tgcgtagatg
     2461 gttcctgcaa tggggtggtt tagtttcgca ttaggggtgt tttttttcgt tcctgcatta
     2521 agttttgctg aaaatttcga tgaaatttca gccgaaattg tgtcgcccac gcttatcgca
     2581 tcggaaattt ttcattcctg ggaatatcaa attccctccg gctatcgcat taaaacgcac
     2641 ggcactgaca gcacaactga ggggcaacgc aagtcgccaa ccaatgcggc actcactcgg
     2701 aaaacccagt ctgaagtact ccccatattc actgagctga tgaaggaagt tcaattaatt
     2761 cgaacagatc aacaaaaaga gaacttcctt cgcggtgctt tgtacttggc gaaaaaa
//
LOCUS       DAOMXO010000292         2817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20316_length_2817_cov_4.760319, whole genome shotgun sequence.
ACCESSION   DAOMXO010000292 DAOMXO010000000
VERSION     DAOMXO010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20316_length_2817_cov_4.760319"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2187
                     /locus_tag="PLH57_06825"
     CDS             <1..2187
                     /locus_tag="PLH57_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRK02364.1"
                     /translation="LSDLAVILGLAGLVTLIFSRIRQPVVLGYIVAGLIVSPASSPFK
                     LISDQPNVRVWADLGVIFLMFSLGLEFSFRKLSRVGISALLTALIEVTGVMLLGYCVT
                     RYGLGWRKTDSVLAAAMLSISSTTIILKALDDLRLKTRRFAEMIFAILIVEDLIAIMV
                     LVGLAMVAGGEGVRGLDLLFAAGKLFIVVGSWVVFGYFIVPRLMKFVGRLGGEEMLIV
                     VSLALCLGLSVFAGSFGYSVALGAFVMGSILAESSESHRISESLLPIKNVFAAIFFVS
                     VGMLIDVKVLQTNLGTIALLSALVVGGKFLFASMGSLVSGQTLRTSVQVGMGLGQIGE
                     FSFIIVGTGLLLGLGNSKLYPIAVAVSVITTFLTPLLVRVSHKTAVRIEHWLPHETKK
                     FLVRYAAWAQSRRADRQEQVEYLQGFLRIATNAIVVTVVFFLSGEIVLPRAIAHFPQL
                     KPLLHSALAWGVALLLALPFIWGMFSSFTEKKTKHTGPRIVGHFATVVWLGLLSLRFF
                     PIGIVLLVMAALVGVVVIFLHKELAASYRWFEKRFLDTFKDDPKTRRSKDLLRDLAPW
                     DAHLVRIKVHANSDVVGLSLQEVGLREKHGVNVVVIQRGTRAIVPPASNEKIYPKDEL
                     LILGTDEEVEPLRQRLERPPGLRERYQNVEGYELKELLVSERSPLVGLTIKDSGIRER
                     FGGMVVGLERDGERTINPTSIHHIQSGDVLWVVGENEKLRALIDWA"
     gene            complement(2201..>2817)
                     /locus_tag="PLH57_06830"
     CDS             complement(2201..>2817)
                     /locus_tag="PLH57_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRK02365.1"
                     /translation="PRIPRWKDLAGLQNPNFLNVDLFVEHFAGPCSAILGERLGPVLL
                     EFTAFRDEIVTLGPELGHRISQFVKAVNLRFPEIQFAVEFRNRALAVPALFECLEPAV
                     PAMTSWTRMIAIEEQWTRLLKLKRNWPFLMARPVLRPGKLKADADAQYSPFGRLHERI
                     TPMRATLIDMWQFARAHNIPSYFMISNTIEGCAHRTIAEIVESS"
BASE COUNT          613 a          644 c          767 g          793 t
ORIGIN      
        1 ctatcggacc tggccgtcat tttagggttg gccgggctcg tgacgcttat attcagccgc
       61 attcgccagc ccgtggtgtt ggggtacatt gtggccggac taattgtcag ccccgcttct
      121 tctcctttta aactcatcag tgatcagccg aacgtgcgcg tgtgggcaga ccttggcgtt
      181 atatttctaa tgttttcgtt gggtctcgaa tttagttttc gcaagctctc acgggtcggc
      241 atttccgcac tgttgaccgc gttgatcgag gtcaccggcg ttatgttgtt gggttattgc
      301 gtgacccgtt acggtctcgg atggcgaaag accgatagcg tgttggccgc ggccatgctt
      361 tctatttcat cgacaacgat tattctcaaa gccctcgacg acttgcgact taaaacgcgg
      421 agatttgccg aaatgatttt cgcgattctg atagtcgaag atttgatcgc catcatggtg
      481 ctcgtgggac ttgcgatggt cgcaggcggc gagggcgtgc gcggtctcga tttgcttttt
      541 gcagctggaa aactattcat cgttgtgggt agttgggtgg tcttcggtta ttttatcgta
      601 cctcgtctga tgaagttcgt gggtcggctt ggtggcgaag aaatgttgat tgtagtttcg
      661 ctcgcgcttt gtctaggact ctcggtgttt gccggctcat tcggttattc ggtggctctc
      721 ggtgcgtttg ttatgggatc aattctggcg gaaagttcgg aatcccatcg aatttctgaa
      781 tcgttgttac cgataaaaaa cgtgtttgcc gcaatatttt tcgtctcggt cggcatgtta
      841 atagacgtga aagtacttca aaccaaccta ggtaccattg ctctgttgtc ggcgttggtg
      901 gtggggggca agttcctctt tgcatcaatg ggatccttgg tgtccgggca aactctaaga
      961 acctcggtac aagttggtat gggtctcgga caaataggtg agttttcatt cattatcgta
     1021 ggcactggcc tattattggg cctcggaaat tcaaagcttt acccgatcgc tgttgcggtg
     1081 tcggtcatta cgacctttct taccccttta ttggtaaggg tttcccataa gacggctgtt
     1141 cgaatcgaac actggttgcc tcatgaaact aaaaaatttc tggtgcgtta tgcagcatgg
     1201 gcccagtcgc gtcgcgccga tcgccaagaa caggtcgaat atctccaagg attcttgcga
     1261 atcgctacca atgcgattgt agtgaccgta gtattttttc tttccggtga gatcgtactt
     1321 ccgcgggcta ttgcgcactt tccacaactg aaaccgctgc tgcattccgc cttggcctgg
     1381 ggcgtagcac tcttgctcgc gctgccgttc atttggggga tgttttcgag tttcaccgag
     1441 aaaaaaacaa agcacaccgg accccgcata gttggtcatt tcgctactgt tgtgtggctg
     1501 ggattgctga gtcttcgttt ttttccaata ggtatagtgc ttttggtaat ggcggcgtta
     1561 gtcggagttg tggtgatttt tcttcataaa gagctcgctg cttcttatcg ttggtttgaa
     1621 aaaagatttt tggatacctt caaggatgat cctaaaacgc ggcgatcaaa agacctgttg
     1681 cgggatttag cgccctggga tgctcacttg gtgcgtatta aggtacatgc gaattcagac
     1741 gtcgtcggac tgtccttaca ggaagtcggc cttcgggaaa aacacggcgt gaacgtcgtg
     1801 gtgattcaac gtggtacccg agcaattgta ccaccggctt cgaacgaaaa gatctatcct
     1861 aaagacgaac tacttatttt aggcactgac gaagaagtgg agccgcttag gcagcggttg
     1921 gagcgcccac ctgggctgcg tgaacgttat caaaacgtcg aaggctatga attgaaagag
     1981 ttgttggtga gcgaacgatc acctcttgtg gggctgacga tcaaagactc gggcattcgc
     2041 gaacggttcg gcgggatggt agttggtctt gaacgcgacg gcgaacgtac aattaaccca
     2101 acttcgattc atcatataca gtctggggac gtgctgtggg tcgtgggtga aaatgaaaaa
     2161 ttacgcgccc taatcgactg ggcgtgaacg cttcggtgtt ttaggagctt tcgacgattt
     2221 ccgcaatcgt acggtgggca cagccctcaa tggtgttact gatcataaaa taacttggaa
     2281 tattgtgagc gcgtgcgaat tgccacatat caattaaagt tgcccgcatt ggggtaattc
     2341 gctcatggag ccggccgaat ggcgaatact gtgcatcggc atcagctttt aactttcctg
     2401 gtcgtaaaac tgggcgggcc attaaaaatg gccaattccg ttttaacttt agcaaacgtg
     2461 tccactgctc ctcaattgca atcatgcgcg tccaacttgt cattgcgggg actgcgggtt
     2521 caagacattc aaataaagca ggtaccgcga gcgctcgatt gcgaaactct acggcaaatt
     2581 gaatttcggg aaaacggaga tttacggcct tcacgaactg cgaaatgcga tgccccagtt
     2641 ccgggcccag cgtcacgatt tcatctctaa atgcagtgaa ctccaaaagc accggcccca
     2701 agcgttcccc gagaatggcg ctgcaaggtc ctgcgaaatg ttccacgaat aagtctacgt
     2761 tcaggaagtt agggttctgg agtccggcga ggtctttcca acgaggaatt cttggga
//
LOCUS       DAOMXO010000293         2814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20360_length_2814_cov_4.407394, whole genome shotgun sequence.
ACCESSION   DAOMXO010000293 DAOMXO010000000
VERSION     DAOMXO010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20360_length_2814_cov_4.407394"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLH57_06835"
     CDS             <1..397
                     /locus_tag="PLH57_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRK02366.1"
                     /translation="VPSTVSRIGDAMPGLGIVAAVLGVVLTMGKLTQGKEVIGHSVAA
                     ALVGTFIGILLSYGLFQPISAKIEGNLNAEGDYIKCIKACLLAFAKNCGPKVCVEFSR
                     RTIPPEVRPTFQEVDAATSSGGGKEAKAA"
     gene            401..1195
                     /locus_tag="PLH57_06840"
     CDS             401..1195
                     /locus_tag="PLH57_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRK02367.1"
                     /translation="MAGGGEIIVIKKKKAAGHGGAHGGAWKVAYADFVTAMMAFFLVM
                     WLMGADEETKQAVSHYFNHPNTPYKQGGDPQSNAAHPMGESEGHGDNVLAGASGLFPE
                     DLIDRPIPHRDLMKENVTISKMIEDLLEGNVFGMDVTENQIKFSLPEHVLFDAGSATL
                     HPNVDKYMALLGNVLKSFKGYVRIEGHSDDTPTNGTRFANNYELSMARAVSVMQYLVK
                     NHGMGEDRVIPTGAGAREPLVPNTSVENRAKNRRVELTLMYEYPSN"
     gene            1250..2011
                     /locus_tag="PLH57_06845"
     CDS             1250..2011
                     /locus_tag="PLH57_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02368.1"
                     /translation="MGNPSIFRTLVFVIVSLVWNSPTSHAMNSRTKLCAAVISAALCG
                     GTAHCAYHDLHTPPERSSDFVKTTERGTLIKLPEYFTVRNQWLAAEKNLVVEDADHSY
                     GEITTFLHRYTHHYRHKRKNGRFLSEATVRGIPGSNGGYEIAVQVNDDLVLGRVLELP
                     VKGNSSSLVRYEIRASDGSLVAASKKIPRSSDQVDFTLQNGNESFTIRRVDDGIIRER
                     WSIERFTPKIQETVDSRLVLFFVAYKQAMDEPVRE"
     gene            2008..>2814
                     /locus_tag="PLH57_06850"
     CDS             2008..>2814
                     /locus_tag="PLH57_06850"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HRK02369.1"
                     /translation="MNLPSYFNSQLFILAVALICSACGHKPFDKAVREFSDPRILALE
                     KTAPPSPEDFADDLNYKSLPRALESSIQVLKSRPDATLMFGDREISAQHYAEALNNFS
                     TFLQNAADANLSEQTVYKYVIENFDFYQVYGDRAPSQIFMTSYFEPVIEGSLRRTKRF
                     SQPIYSRPADLLELDVAAFDEGLAPPRKYRARVENGKVVPYYSRREIDIDMALAKKKL
                     ELLWVDPIDSFIAQIQGSATVVLPSGKQVRINYAERNGHKYEALGKFLKAH"
BASE COUNT          739 a          728 c          657 g          690 t
ORIGIN      
        1 agttccatcc accgtatcgc gaatcggtga cgccatgcct ggtctcggaa tcgtggccgc
       61 ggttctaggt gtggtactca cgatgggaaa actgactcaa ggtaaagaag taatcggtca
      121 tagtgtggcc gccgcactcg tgggaacttt catcgggatt cttttgtcct acggattgtt
      181 ccaaccgatt tccgcaaaaa tcgaaggtaa tctcaatgcc gaaggtgatt acataaaatg
      241 catcaaagcc tgtctgctgg cttttgcaaa aaactgcggt cccaaagttt gcgtagagtt
      301 ttcacgacgt acgattccac ccgaagttcg tccgaccttt caggaagtcg acgcagcaac
      361 tagttctggt ggcggcaaag aagcaaaggc ggcatagccc atggctggcg gcggcgaaat
      421 cattgtcata aaaaagaaaa aagccgcagg tcacggtggc gcccacgggg gtgcttggaa
      481 ggtggcttac gccgacttcg ttactgctat gatggcattt ttccttgtga tgtggctcat
      541 gggtgccgac gaagaaacca aacaagccgt gtctcattat ttcaaccatc caaatacgcc
      601 ctacaaacag ggcggagacc ctcagtctaa cgcagcccat ccgatgggcg aaagcgaagg
      661 acacggcgac aatgttctgg caggcgcttc gggattgttt cctgaagatt taattgatcg
      721 tcctattccg catcgtgatc tgatgaaaga aaacgtcacg atcagtaaaa tgattgaaga
      781 cttactcgaa ggaaatgttt tcggaatgga cgtgactgaa aatcaaatca agttttcgct
      841 gcccgaacac gtgttgttcg atgccggcag cgcgaccctg caccccaacg tcgacaagta
      901 catggcgtta ttgggtaacg tcttaaagag cttcaaaggc tacgtaagaa tcgaaggtca
      961 cagcgacgat acccctacga acggcacacg tttcgccaac aattacgaac tttccatggc
     1021 tcgcgccgtt tctgtaatgc aatatctcgt gaaaaatcac ggtatgggcg aagatcgggt
     1081 gattccaacc ggcgccggcg cccgagaacc tttagttccg aacacctccg tcgaaaacag
     1141 agcaaaaaac cgacgcgtgg agctcacctt aatgtacgaa tacccatcca actagttatt
     1201 cgaagcgctc tattgactga ctgcgttact atggtaaaga cccgcgccta tgggcaaccc
     1261 aagcattttt cggaccttgg ttttcgtgat cgtttcattg gtatggaact cacccacctc
     1321 ccacgccatg aattctagaa ctaaactctg tgccgccgtt atttcggccg cgctatgcgg
     1381 gggtaccgca cattgtgcgt accacgactt gcacacccca cccgagcgct cgagtgattt
     1441 cgttaaaacc accgagcgtg gcacactaat caaacttccg gaatatttta cagttcgcaa
     1501 ccagtggctc gcagcggaaa aaaacctcgt tgttgaagac gccgaccata gctatggcga
     1561 gatcaccact tttctccatc gctacactca tcactaccgc cacaaacgca aaaatggccg
     1621 atttctttcg gaggctaccg ttaggggaat tcccggttcc aacggcggtt acgaaatcgc
     1681 ggtgcaggtc aacgacgatc tggttctcgg tcgcgtattg gaactacccg tcaaaggtaa
     1741 ttcctcttca ctcgttcgtt acgaaattcg ggcgagcgat ggttcgttgg tcgccgcctc
     1801 aaaaaaaata ccgcgatcaa gtgaccaggt tgattttacg ctccaaaacg gaaacgaatc
     1861 ttttactata cgtcgcgtcg atgacggcat catccgcgag agatggtcga tagagcgttt
     1921 tactcccaag atacaggaga ccgttgattc ccgactagtc ttattttttg ttgcgtacaa
     1981 acaagcgatg gatgaacctg taagggaatg aaccttccct cttattttaa ttcgcaacta
     2041 ttcattcttg cggtcgctct aatatgctcc gcctgtggac ataaaccctt cgacaaagca
     2101 gtgcgcgagt ttagtgatcc aagaattttg gcactggaaa aaactgcgcc accttcgccc
     2161 gaagattttg ccgacgactt aaactacaag agcctgcctc gggctctcga atcaagtatt
     2221 caagttctaa aatcgcggcc cgatgccaca ttgatgtttg gcgatcgtga gatttcggca
     2281 caacattacg ccgaggcctt aaataatttt tctacgtttc ttcaaaacgc cgcggacgcc
     2341 aatctttctg aacaaacggt ttacaaatat gtaattgaaa atttcgactt ctatcaggtt
     2401 tacggagatc gtgcgccctc ccaaatcttc atgacctctt attttgaacc cgtaattgaa
     2461 ggcagccttc gacgtaccaa acgattttcg cagcctattt attctcgtcc cgccgatctt
     2521 ttagagctag acgtagcggc cttcgacgaa gggctcgccc cccctcgtaa gtatcgagct
     2581 cgggtagaaa acggcaaagt agttccgtac tattcaaggc gcgaaattga catcgacatg
     2641 gctcttgcta agaaaaaatt ggaacttctt tgggtagatc cgattgattc gttcattgcc
     2701 caaattcagg gttccgccac cgtcgtatta cccagcggca aacaggtgcg cattaattac
     2761 gcagaacgta atggtcacaa atacgaagca ctcggcaaat ttcttaaagc tcat
//
LOCUS       DAOMXO010000294         2783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20679_length_2783_cov_4.291422, whole genome shotgun sequence.
ACCESSION   DAOMXO010000294 DAOMXO010000000
VERSION     DAOMXO010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20679_length_2783_cov_4.291422"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /locus_tag="PLH57_06855"
     CDS             <1..1738
                     /locus_tag="PLH57_06855"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRK02370.1"
                     /translation="IGAISEGALGTFLHNEWTRMGGMDYSDVGYDAFLINGKRESDLL
                     EAKAGESIRLRIINAAASTYFHIALGNQNMKVISADGIDVAPIRAREILIGMAETYDL
                     LFKLPDSKKYELKITAQDGTGTASTWIGSGKKEFAPIKPKPPLYESMDHSAHANHGQA
                     NHGQGEPDDSHANHGQAQHGQGEPDDSHAHHGQGKPDDSHATHGQAHHGQAHHGQGHH
                     GQGEPDDSHANHGQAHHGQGHHGQGKPDEGESDVVSTLTVDNLKSPTKTSFAKNAKVH
                     NVKFVLGGDMERYVWHLNGKAIHQDRTVEINEGDVVRFTFENQTMMHHPMHLHGHFFR
                     VLGAQGERAPLKHTVDVAPHSTRTIEFLADEPGEWMLHCHNLYHLKTGMARVIKYSSF
                     KPKKEMLHWQKHDPHLHDHWYFYGMLEAASNHGQAYFRGSQTWNQVELRVETANVAGK
                     NFSFGERWDYEGDLFYRRWIDSNLNLIGGATSFGREVRAALGVGYRLPLLIESNLLVN
                     HSGRIRLDLEKRFQWAKTIFSDVDFSWRPNQKGDHAAEFEVTLMYSPAWAWAVGAMIT
                     NDSIGGGIQVQF"
     gene            complement(1759..2325)
                     /locus_tag="PLH57_06860"
     CDS             complement(1759..2325)
                     /locus_tag="PLH57_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02371.1"
                     /translation="MPHLLLALIVVLSTASFATASSLNQAVSCSYIITGSDDDLASWR
                     SDIYLERIRPGQTTSDRQEILRMARALVRDIKKDAVLHPQLVNGFVFSSELMGVLRMA
                     DDHSTYVNYNNNYGAHLYVEIAEIIEQEITPNELELAAIEDLSLGLDISGYWALDEKK
                     GDLDQATLKQLHLRLDTVRNKLLAKGRK"
     gene            2530..>2783
                     /locus_tag="PLH57_06865"
     CDS             2530..>2783
                     /locus_tag="PLH57_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRK02372.1"
                     /translation="MKLSCVWNEKMQFDAKTGAHSVRMDAKSPIGNDSGPSPKQLLLA
                     SICGCTAMDVAALMRKHKQSMERFEVEADAELTEGSYPVV"
BASE COUNT          755 a          634 c          735 g          659 t
ORIGIN      
        1 gattggtgcg attagtgaag gagcgctagg tacgtttctg cataacgaat ggacgcgaat
       61 gggtggcatg gactattccg acgtaggata cgatgccttt ctgatcaacg gcaaacgcga
      121 gagtgattta ttagaggcca aagcgggaga gagcataagg ctacgaatta tcaacgccgc
      181 ggcttcgacg tattttcata tagcactggg aaatcagaat atgaaagtca tctcagccga
      241 tggaatcgac gttgctccga tacgagcgcg agaaattctg atcggaatgg ccgaaactta
      301 cgatttactt tttaagttac cggactcaaa aaagtacgaa cttaaaatca ctgcgcaaga
      361 tggtaccggt acagcgtcta catggattgg atctggaaaa aaagaattcg cgcccataaa
      421 accaaaaccg ccactttatg aatccatgga tcactccgcc cacgccaacc acggccaagc
      481 caaccacggc caaggtgagc ctgacgattc ccacgccaac cacggccaag cccaacacgg
      541 ccaaggtgag cctgacgact cccacgccca tcacggccaa ggaaagcctg acgactccca
      601 cgccacccac gggcaagccc accacggcca agcccatcac ggccaaggcc accacggcca
      661 aggtgagcct gacgactccc acgccaacca cgggcaagcc caccacggcc aaggccacca
      721 cggccaaggt aagcctgacg aaggtgagtc tgacgtcgtt tcgactctca ctgtcgacaa
      781 tttaaaatct ccaaccaaaa cgtcgtttgc aaaaaatgcg aaagtccaca atgtaaaatt
      841 tgttttgggt ggcgatatgg aacgttatgt gtggcattta aacggcaagg caatacatca
      901 agatcgtact gtcgaaatca acgaaggtga cgtggtgaga tttacgtttg aaaatcaaac
      961 gatgatgcac cacccgatgc atttgcatgg gcactttttt cgagtgttgg gtgcgcaggg
     1021 tgaaagagct ccgctcaaac acacggttga cgttgctcct cattcgactc ggaccattga
     1081 gtttttagca gacgaacccg gcgagtggat gttgcattgt cacaatcttt accatctcaa
     1141 aactggcatg gcgcgcgtaa taaagtattc ctcgtttaag ccaaaaaaag agatgcttca
     1201 ctggcagaaa cacgacccgc acttgcatga ccattggtac ttttacggaa tgctcgaggc
     1261 cgcatcgaat catggtcagg cttactttcg aggatcgcaa acctggaatc aagttgaact
     1321 cagagtggaa acggccaatg tagctggcaa aaacttttcg tttggcgaac gctgggacta
     1381 cgagggcgat ttgttttatc gtcgttggat agattcgaat ttaaatctaa tcggcggtgc
     1441 tacaagtttt ggtcgcgaag tgcgagccgc gctgggagtc ggatataggc ttccgctgct
     1501 gatcgagagt aatttgttag tgaatcatag cggacgaatt cggctagatc tggaaaaacg
     1561 attccaatgg gccaaaacaa ttttttcaga tgtagatttt agctggaggc caaaccaaaa
     1621 aggtgaccac gctgcggagt ttgaagtgac gttgatgtat tcaccagctt gggcatgggc
     1681 tgtaggagct atgatcacca acgatagtat cggaggcgga attcaagtgc agttttaagg
     1741 gcgtctgagt gtattcggtt atttgcgacc tttggcgaga agcttattgc gaactgtatc
     1801 gagacgtaag tggagttgtt tgagagttgc ctggtctaga tcgccttttt tttcgtccaa
     1861 tgcccagtag ccggatatgt cgagccccaa cgaaagatct tctattgccg ctagttctaa
     1921 ttcgttaggg gtaatttctt gctcaataat ttcagcgatt tcgacgtaaa ggtgtgcgcc
     1981 gtaattgttg ttataattga cgtaggtaga atgatcgtct gccattcgaa gcacgcccat
     2041 tagttcgcta gaaaaaacga aaccattaac aagctgcggg tgcaagaccg cgtctttttt
     2101 gatgtcgcga acaagtgcgc gggccatgcg aagaatttcc tggcggtcgg aggtcgtttg
     2161 gccggggcga atgcgttcaa ggtaaatgtc acttcgccag ctggcaaggt cgtcatccga
     2221 acctgtaatg atgtagctgc aactcaccgc ttggttaagg ctcgatgccg tggcgaaaga
     2281 agctgtcgag agaacgacta tcagtgctaa taaaaggtgg ggcatcggct acctcgctaa
     2341 caaatgcttt taaccgggga tccgctcttg cgaacggctt cgtaatacat ttggatattg
     2401 tgcgtcaagt tgctcaagaa actacttaca tcgggcgcat ttgtttttaa tccggggtct
     2461 aagaccgtgt agggaagtgt tgaactactg ggaatagggg attactctag ccagagaata
     2521 ggaaatacta tgaagcttag ctgtgtttgg aacgaaaaga tgcagtttga tgccaaaact
     2581 ggtgcacatt ccgtgcgtat ggatgcgaaa agcccgatag ggaacgattc tggacctagt
     2641 cctaagcagt tgttgctcgc gagcatatgt ggctgcaccg caatggacgt cgcggcatta
     2701 atgcgaaaac ataaacagag catggagcgg tttgaagtgg aagccgatgc ggagttgacc
     2761 gaaggttctt atccagtggt gtt
//
LOCUS       DAOMXO010000295         2779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20711_length_2779_cov_3.990088, whole genome shotgun sequence.
ACCESSION   DAOMXO010000295 DAOMXO010000000
VERSION     DAOMXO010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20711_length_2779_cov_3.990088"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLH57_06870"
     CDS             <1..623
                     /locus_tag="PLH57_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02373.1"
                     /translation="FEPVFFGETEEAPEPVRPHVNDYGVSLELNPLRTSASSIRLDVG
                     TDFENRLISRKNRVLLGGGVNGQGVDLRASLVVPIRTSDSLQAYLKGFADLGLAKGSL
                     SADEEAHIQLLARIGASLGIDLHNRNTWSVGGFIGGGPVIDVLGTKDPVSIDVLLGVS
                     GTFGGDEHVRRDLAAEAASVEESSLPCINSAVCYHPHLWVGFGTFF"
     gene            578..2143
                     /locus_tag="PLH57_06875"
     CDS             578..2143
                     /locus_tag="PLH57_06875"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRK02374.1"
                     /translation="MLSPPLMGGFRYIFLIFAVTLATAGCPETSPRKIETSCDLLDDA
                     LVFAHTRHLTRTTNAERFTRFAGALNRLYTQSGLPIVPSTPKSPDELAEICKTARMDF
                     RNSRFKSFSEETLVLSILDIYLRSLDHHSRVEAREEDIEVPAMLGLRIDLQHMPPRII
                     EVIAGSAAHRAGIPENGLILSINQRDTTQLSPKDFAALKDELAPSNESLELVIEKQDG
                     VVRTYNLAIEFFVESAVSSKLIPTDTGVVGYIRLRRFNRFEDKRALAEAVKSYQDASS
                     VVSALILDLRDNPGGSVEGAAEVANHFVLNGGVFARFSDASGQVIHEEEFPYRVSRTW
                     DGPLIVLVNWATRSAAELLAAALQDYGAVVVGESTFGQGTSHTGFDLGKEFGLNGKAQ
                     ITDAYNHRPSGATTQLDGVEPDIKLNLSKRDPDAELFRESQYKSPNAIARPTYKIEPL
                     RGFDSNIRTRSKKFLDGLRKFHGKLASKPTGKSKKKRKTKAYSNDLGIDTAAKIGEYW
                     CSLENPDRCKAPR"
     gene            complement(2160..2561)
                     /locus_tag="PLH57_06880"
     CDS             complement(2160..2561)
                     /locus_tag="PLH57_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRK02375.1"
                     /translation="MQAVITLVGRNFLALIFLSSGVSKIAKYSDTVAYMQQKGMPAAP
                     FFLFAAVLIELSAALALITGYRARTGALLLVLFLVPTTYIFHYKSAFDAQMNVIDQIQ
                     MISLLKNIAIIGGLLGVYANGPGPWALGKKS"
     gene            complement(2593..>2779)
                     /locus_tag="PLH57_06885"
     CDS             complement(2593..>2779)
                     /locus_tag="PLH57_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02376.1"
                     /translation="ALRRCEKVLSDSFLRLALSQIDLYNLVELSSLRSDARSSVEEAV
                     GCDPNNVRARRLLQKLR"
BASE COUNT          709 a          769 c          676 g          625 t
ORIGIN      
        1 ggttcgaacc tgtgtttttc ggtgaaacag aggaagctcc tgaaccagtt cgccctcacg
       61 tcaatgacta cggcgtttca ctcgaactaa acccactgcg cacgagtgca tcttccattc
      121 gactcgacgt tggcaccgac ttcgagaatc gtctaatttc tcgaaagaac cgggtactgc
      181 tgggcggcgg cgtcaacggc caaggcgtgg atctcagagc ctcactcgtg gttcctatac
      241 gcacgagcga ctcattgcag gcctatctca aaggtttcgc cgatctcgga ctcgccaaag
      301 gtagcctgag cgccgacgaa gaagctcaca ttcaattgtt ggctcgaatc ggcgcttcac
      361 tcggaatcga ccttcataac cgaaacactt ggtccgtggg cggctttatc ggaggcggtc
      421 ccgtgatcga tgttttaggt accaaagacc ccgtcagtat cgacgtacta ttgggcgtct
      481 cgggcacttt tggtggcgat gagcacgttc gccgtgacct agcagccgaa gcagccagcg
      541 tagaagaaag ttccttgcct tgcattaata gcgcggtgtg ttatcacccc cacttatggg
      601 tgggtttcgg tacatttttc taatttttgc ggtgacgctc gctactgcgg gatgccccga
      661 gacaagtccg cgcaagatcg aaacctcgtg tgatctactc gacgacgcgc tggttttcgc
      721 gcacacgcgc catctgacgc gaaccacgaa cgccgaacgt tttacccggt ttgcaggcgc
      781 cctgaaccga ctttatacac agtcaggact cccaatcgtg ccctctactc caaaatcacc
      841 cgacgaactt gctgagatct gcaaaacggc gcgcatggat ttcagaaaca gtcgcttcaa
      901 aagtttttcg gaagaaactc tagttctatc gattttggac atctatttac gttctctcga
      961 ccaccattcc cgcgtagagg cccgcgagga agatatcgaa gttcccgcca tgctcggcct
     1021 acgcattgac cttcaacaca tgcctccgcg catcatcgaa gtgattgcgg ggtcggcggc
     1081 ccaccgcgcc ggcatacccg aaaacggatt gatactttcg atcaaccagc gcgacaccac
     1141 gcaattatcg cccaaagatt tcgccgccct caaagacgaa ctcgctcctt cgaacgagtc
     1201 gctcgagctc gtgattgaaa aacaagacgg cgtggtgcgc acctacaact tggcgattga
     1261 gtttttcgtg gagtcagccg taagttctaa acttattccc acggacacgg gcgtcgtcgg
     1321 ctacattcga ttacgccgct tcaatagatt cgaagacaaa cgagctctcg ccgaagcggt
     1381 aaaaagttac caagatgctt ccagtgtcgt atcggctttg attttagacc tgcgcgacaa
     1441 tccgggaggt tccgtagaag gagctgccga agtagcaaac cacttcgttt taaatggagg
     1501 ggtttttgcg cgctttagtg atgccagcgg tcaggtgatc catgaagaag agtttcccta
     1561 tcgcgtgagc cgcacctggg acggtccgtt gattgttctc gtaaactggg ccacccgttc
     1621 ggcagccgag cttcttgcgg ctgctctaca agactacggc gcggtggtcg taggcgaatc
     1681 gacctttggc cagggaacta gccacaccgg attcgatctc ggcaaagagt ttggcctcaa
     1741 cggtaaggct cagatcactg acgcgtacaa ccaccggcca agtggcgcaa ccactcaact
     1801 cgacggtgta gagcctgaca tcaaactaaa tttatctaaa agagatccag acgccgaact
     1861 atttcgcgaa tcccagtaca agtcgccgaa cgccattgcg cgcccaacct acaagatcga
     1921 acctcttcgt ggctttgatt ccaacattcg gactcgctcc aaaaaattcc tcgatggtct
     1981 gcgaaagttc cacggaaaac tagcgagcaa acctacgggg aaatctaaga aaaagagaaa
     2041 aacgaaggcc tacagtaacg atcttggcat cgacactgct gcgaaaattg gcgaatattg
     2101 gtgctcgctt gaaaacccgg atcgctgcaa agctccgcga taataagaat aattaaagtt
     2161 tagcttttct tccccaatgc ccatggaccg ggaccgttgg cgtatacccc taacaggccc
     2221 ccaataatcg caatgttttt tagcaagctg atcatctgaa tttgatcgat cacgttcatt
     2281 tgcgcatcaa aagctgattt atagtgaaaa atataggtcg ttggcaccag aaacagcact
     2341 agcaacagag ctcccgtacg ggcgcgatag cccgtgatca gcgccagggc agccgacaac
     2401 tcaattaata cggcggcaaa cagaaaaaac ggagcagcag gcataccctt ctgctgcata
     2461 taagccacgg tgtcggaata tttcgcgatt tttgaaacac cggacgacaa aaaaataagc
     2521 gctaaaaagt tgcgacccac tagagttatc acagcctgca tgaacacacc tctctcgatg
     2581 tagattttcg ttttaacgca atttctgtag taatcggcga gctcgcacgt tgttcggatc
     2641 gcaacccact gcttcttcga cagaggaccg cgcatcactc ctcaacgaac tcagctccac
     2701 aagattgtat aggtcgatct gggaaagtgc tagtcgaaga aacgaatccg acaaaacttt
     2761 ttcgcatcgg cgcaacgcc
//
LOCUS       DAOMXO010000296         2778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20721_length_2778_cov_4.989350, whole genome shotgun sequence.
ACCESSION   DAOMXO010000296 DAOMXO010000000
VERSION     DAOMXO010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20721_length_2778_cov_4.989350"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1924
                     /locus_tag="PLH57_06890"
     CDS             <1..1924
                     /locus_tag="PLH57_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02377.1"
                     /translation="GQMITELDAIPVDGGEKVANAVFPKVTQLVRIVTNSLITQEQKL
                     IIFEQLNILKNNVTDYQAAVEKGTALRNDRIDALTKVESLTKKLIETQARNIMNFGSV
                     LQPELVHALIGSAAFILAALLWLFGMSGRFSRVVAGDAKRIRETLEKWRTQGTAPETN
                     SNIKYGDFGSIFDEIKKLNESLEKKNEAEDCLRTHGVTPQVVVNTKKNAVLWNSAFSG
                     LAKQRSFDELGAVSYSRLLRFTDGRTQPVDPVEKCMIDGKEVSQLCMLRVGSELVPTY
                     VQCTPVFESADVISNVVVELRDLRAEQRRIETEIERQVSFIESALDAIKEGRVPEEGA
                     IGAKQSIISFIGKLRQFALREQEKHEVLIGQIELLWTRVEKEADLGRRTIDQVDGVRS
                     QVVTLGSTLKEVCEQAKNLRWMQEKSGVITGDVQKRMDSLRRQFETLATEMNRSKRML
                     TGATTSLSKSQTILSKVRVLQHAIESILKRSSVLNANNAILGAQTLSANDVVSISENI
                     SEVLHQFERGYTQLSNVVTLLERGFKDSNERVLQDLQVCLRIIGDDEQLIREVKGLEQ
                     AHEKSFEDLARVFATAKTLEGAAVRTAEALTNVAQTTEALAQLGQTSQDLHTQVEEGL
                     RGVLGHFSEEFRRNSK"
     gene            complement(1895..>2778)
                     /locus_tag="PLH57_06895"
     CDS             complement(1895..>2778)
                     /locus_tag="PLH57_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02378.1"
                     /translation="SYNELEGISGASGSEVISKATGLIGKNQHGRIQNLTGYFDPKVY
                     DDFGGAIGFLTSFQTNLLIRPTTDIDVQSVLDSGISIGAAKSYFHKRLSLGINIHMLY
                     RMGVDKVIPAIDILSGRSLKPREYAGQGIGFDADFGGYYQIPWEPRFMRLNVGGSING
                     LLQSNFNTMSGEIIDGVKTAPPRQDRTVNLGLRADFPDSKILAAPLIALEVQNLGSIN
                     KRYSFLKRLHFGGEARVSRRVALRTGLYQGYPSGGVGINLPVVKLDFATYVEELGGVA
                     AEWPDRRYLFRIAAELL"
BASE COUNT          752 a          624 c          732 g          670 t
ORIGIN      
        1 gggccagatg atcaccgaac ttgatgctat tccggtagat ggcggtgaga aagttgcgaa
       61 cgcggtattc cccaaggtca cgcaattggt tcgaattgtg accaactcac tgatcacgca
      121 agagcagaaa ctaatcattt tcgaacaact caatattctt aaaaacaatg tcactgacta
      181 tcaggcagct gttgaaaagg gcacagcgct tcgaaacgac agaattgatg cgctcaccaa
      241 ggtcgagtca ctcacgaaaa agcttatcga gacgcaggca cgaaacatca tgaatttcgg
      301 tagtgtactt caaccggaac tggttcatgc attgattggt agcgccgctt ttatacttgc
      361 cgctctcttg tggttatttg gaatgtccgg gcggttcagt cgtgtggtcg ctggcgacgc
      421 aaaacgtatt cgcgaaacac ttgaaaagtg gcgcacccaa ggaaccgctc cggaaacgaa
      481 ttcaaatatt aaatacggag acttcggttc aatatttgat gaaataaaaa agcttaacga
      541 atcactggaa aagaaaaatg aagccgaaga ctgtttgcgt actcacgggg tcacgcccca
      601 ggttgtggtg aataccaaaa aaaatgcggt tttatggaat tcggctttca gtggtttggc
      661 gaaacagcgg agttttgacg aattgggagc agtatcttac agccgattgc tccgctttac
      721 cgacggccgc acccagcctg tagacccggt cgagaaatgc atgatcgatg gtaaggaagt
      781 ttctcagttg tgtatgttgc gagttggcag cgagctcgtt cccacctatg tgcagtgtac
      841 accggtattc gagagcgcag atgttatttc aaatgtggtc gtcgaattgc gcgatctaag
      901 agccgagcag cgaaggattg aaacggaaat cgagcgtcag gttagtttta ttgaatcggc
      961 gcttgacgct ataaaagagg gacgagtgcc ggaggaaggc gctataggag ccaaacaaag
     1021 cattatttct tttatcggaa aacttcggca gtttgctctt agggaacaag aaaaacacga
     1081 agtactcatt gggcaaattg agttgctgtg gactcgtgtc gaaaaagagg ccgatcttgg
     1141 acgtcgcaca attgatcagg tcgacggtgt gcgctctcaa gtggttactc tcggttccac
     1201 gcttaaagaa gtttgcgaac aagcgaaaaa tctgcggtgg atgcaggaaa aatctggggt
     1261 aattaccggc gacgttcaaa agcgaatgga ttctttacgt cggcagttcg aaaccttagc
     1321 gacagaaatg aatcgttcga aacgcatgtt aactggtgcg acgacgtcgc tctccaagtc
     1381 tcaaacaatt ttatcgaaag taagagtatt gcagcatgcg attgaatcga tactcaagcg
     1441 ttcttcggta ttaaatgcga ataatgcgat tttaggggcc caaacccttt cggccaacga
     1501 cgtggtgtcg atttcagaaa atattagcga agtactgcat cagttcgagc gtgggtacac
     1561 tcaactttct aatgtagtga ctctcctaga acgtgggttt aaagacagta acgagcgggt
     1621 tttacaggac ctacaagtct gtttacggat cataggcgac gatgaacagc tgatacgaga
     1681 agtgaagggg ctggaacagg cgcacgaaaa gagttttgaa gacctggcac gcgtgtttgc
     1741 gaccgctaag acgttagagg gagcagcggt tcgcaccgcc gaagccctga cgaacgtggc
     1801 tcagactacc gaagcgttag cacagttggg ccagacgagt caggatctgc acacccaggt
     1861 cgaggaaggt ttgcgcgggg tcctggggca cttttcagag gagttccgcc gcaattcgaa
     1921 ataagtaccg gcgatcaggc cattccgctg cgaccccgcc aagttcttcg acataggttg
     1981 caaaatcgag ttttacgact ggaagattga ttccgactcc gcccgacgga taaccctgat
     2041 acaaacccgt gcgaagtgcc actctccgcg acactcgggc ttcgccgcca aagtgtaacc
     2101 gttttaaaaa ggaataacgt ttgttgatgc ttcccagatt ctgaacctcg agtgcgatca
     2161 gcggggccgc tagaatctta ctgtcgggaa aatctgcccg aagacctagg tttacagtac
     2221 ggtcttggcg ggggggggcg gtcttaactc catctataat ttctcccgac atcgtgttga
     2281 aattggactg caaaagcccg ttgatcgaac cgcctacgtt gagccgcatg aaccgtggct
     2341 cccacgggat ttgatagtat ccgccaaagt ctgcgtcaaa cccgattcct tggccggcgt
     2401 actctcgagg ttttaatgat cgtcccgaaa gaatgtcgat cgctggaatc accttgtcga
     2461 cgcccatgcg gtagagcatg tgaatgttaa taccgagtga gagccttttg tgaaaatagg
     2521 attttgccgc accgatgctt attccggaat ctaaaacaga ttgcacgtca atatcggtcg
     2581 ttgggcgaat taaaaggttg gtttgaaacg aagtcaaaaa tccgattgca ccaccaaaat
     2641 cgtcgtagac cttagggtca aaataccccg taagattttg aatgcgtccg tgttggtttt
     2701 ttccgattaa gccggtggcc ttagaaatga cttcagatcc cgatgcgccc gagattcctt
     2761 ctagttcgtt gtaagatg
//
LOCUS       DAOMXO010000297         2775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20753_length_2775_cov_4.508824, whole genome shotgun sequence.
ACCESSION   DAOMXO010000297 DAOMXO010000000
VERSION     DAOMXO010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20753_length_2775_cov_4.508824"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PLH57_06900"
     CDS             complement(<1..781)
                     /locus_tag="PLH57_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRK02379.1"
                     /translation="MNLPEIAIRKPVFAWMLMSALIIFGGISFMRMGVSQLPDVDFPV
                     VSISATLEGAAPDVIETDVVDVLEDAVMTVEGVRAVGGSARQGSASVVVEFDLDKNID
                     LAVQEVQTKLARAQRELPDDIEPPVVSKTNPEDQPIMWLSVRSAGFSTKELMTYVRDQ
                     LKDRFSTLPGVGEVFLGGYVDPNLRVWISNEKLNRLALTVGDVLSTIRNEHTELPAGR
                     IETYAKEYNVRTIGEASSVEEFKEIPISTRGGQPNYLPIQLG"
     gene            complement(783..2051)
                     /locus_tag="PLH57_06905"
     CDS             complement(783..2051)
                     /locus_tag="PLH57_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRK02380.1"
                     /translation="MCAAFKFVGKSIFALLLLEGAVHAAVDLDGAFKSALKKNETVSI
                     QSEKIEQAAERYGRAKSSFFPTIDGVASYTRLPDTGLTNAFNKPDRPEVKFTANVALF
                     RGGREFAGIRQTKASLAVEQHQSRAIRTELYQAVAESFFETLIHEQDAKNLEIQLSLS
                     KKRENELAERKKIGRSRASELLAVQVAVANLEAQSSSLDALIQSAREKLSYLTGLDRT
                     VKLNDDALISDRSSHEVSALSRYLARVENRPEIAALLQQLEAAEEGVAIARGQHLPSA
                     DVFGNYYLKRTGVNEGQDWDVTARLTLPIFSGGSVLSQVEEAASSKREADLRLANARR
                     VAEQQIRSLWAAVRASQKQMSTLKKALDLAERNYRLQDKEYSLGLVTNLDVIQALNAF
                     YDTRRSYDRVRFQTKLEFIRLETAAALREY"
     gene            complement(2108..>2775)
                     /locus_tag="PLH57_06910"
     CDS             complement(2108..>2775)
                     /locus_tag="PLH57_06910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK02381.1"
                     /translation="HAPSKPGPKGTVVFRQIIESLRKKGHQIEWIEIIGRPNREVIEA
                     IQNCDFVVDELYSDMPLALFAVEAAFYGKPAVVGGYYAEHIGQVLSEQDVPPSLFCAP
                     NSAEAAIEKMIVDPEYRLNLGARAKEFVRNRWSPKQVAGRVLKLIDGTYPREWMYDPS
                     TIRYLEGCGHSAEQGRKTAGAVVALGGPGALCLDDKPELRDLFVQWSAVETKSKLQSE
                     IGA"
BASE COUNT          678 a          723 c          596 g          778 t
ORIGIN      
        1 gacccaattg aatgggcaaa tagttgggct gcccacctcg tgtgctgata ggtatttctt
       61 taaactcctc gaccgaactc gcttccccga ttgtgcgaac attgtattct tttgcgtatg
      121 tctcgatcct acccgcgggg agttcagtgt gttcgttgcg aattgtacta agtacgtctc
      181 ccacagttag tgcgagacga ttaagttttt cattcgaaat ccagacccgc aaattcggat
      241 ctacatatcc acctaaaaaa acctctccca ctccaggtaa agtcgaaaaa cgatctttca
      301 gttgatcccg aacataggtc attagctctt ttgtcgaaaa tccagcactt cgcaccgaca
      361 accacattat aggttgatct tcggggttgg tcttggaaac aaccggaggc tcaatgtcgt
      421 cgggcaactc ccgttgcgcc cgagctagtt tggtctgtac ttcttgcacg gcaagatcaa
      481 tgtttttgtc taaatcaaac tccaccacaa cactggcact accctgccga gcgcttccac
      541 ctactgcccg tacgccctct accgtcatta cggcatcttc gagtacatcg accacatcgg
      601 tttcgataac gtctggagcg gcaccttcaa gggtggcgga aatagacacg accggaaaat
      661 ctacatcggg aagttggctc actcccattc gcatgaacga aatgccgcca aaaataataa
      721 gtgccgacat gagcatccag gcaaaaacag gttttctgat cgcaatttct ggtaaattca
      781 ttttaatatt ctcgcaatgc tgcggcggtt tcgagtcgta taaactctaa tttcgtctga
      841 aaacgaactc gatcatagct ccgtctggtg tcataaaagg catttagcgc ctgaattacg
      901 tcgagattag ttacaagacc taagctatat tccttatcct gaagccgata atttctttca
      961 gcaagatcta acgctttttt tagcgtggac atttgttttt ggctggctcg gaccgcagcc
     1021 cacaaagaac gtatttgctg ttcggctaca cggcgagcat ttgcgagccg caggtcagcc
     1081 tctcttttag aagaagctgc ctcttccact tgtgataaaa ctgagccacc tgaaaaaatg
     1141 ggtaacgtta gtctggctgt tacatcccaa tcttggccct cgttcacgcc cgttcgtttg
     1201 agatagtaat tgccaaatac atcggcactc gggagatgtt ggcctcgtgc tattgcaacc
     1261 ccttcctctg ctgcttccag ttgctgaagc aacgccgcta tttcgggtct attttcgact
     1321 ctggccaaat aacgcgagag tgccgacacc tcatgagaag agcggtcaga gattagtgca
     1381 tcgtcgttta acttcactgt gcgatcgagt ccggtaagat aggacaactt ttcacgggca
     1441 ctttgaataa gagcatcaag actactggac tgtgcctcga ggttcgcaac cgctacttga
     1501 actgctaaaa gttcgcttgc tcgggaccgc cctatttttt ttcgttccgc taattcgttt
     1561 tccctttttt tacttagact caattgtatt tctagatttt ttgcatcttg ctcgtgaatc
     1621 aaagtttcaa agaaactttc ggctactgct tggtatagct ctgtcctaat cgcacgactt
     1681 tggtgctgct caacagccaa cgatgccttt gtttgacgga ttccggcgaa ttcgcgacca
     1741 cctcgaaaaa gtgccacatt tgccgtaaac tttacctctg gtcgatcggg tttattgaag
     1801 gcgttggtta atcccgtgtc cggcaaacga gtataggaag caactccgtc gatggtggga
     1861 aaaaagctgc tctttgcgcg accgtatcgt tccgcagctt gttcgatttt ttcagattgt
     1921 atcgaaactg tctcattctt ctttaaggca ctcttaaacg ccccatcgag gtctaccgcc
     1981 gcatgcacgg ccccttccaa aaggagcaat gcaaaaattg attttccaac aaatttgaat
     2041 gccgcacaca tggcttctaa tcgtagatgc taatattttt attgtcgaat atttcgaacc
     2101 aaaaacgcta tgcgccaatt tcggactgta gtttactttt agtctccacg gcagaccact
     2161 gaacaaacaa gtctctgagt tcgggtttat catcaaggca aagagccccc ggaccgccca
     2221 gcgctacgac agccccagcg gtttttcgcc cctgctccgc ggaatgacca cacccttcta
     2281 aatagcgaat ggtacttggg tcgtacatcc actctctggg ataggttcca tcgattaact
     2341 ttaaaacacg tcctgccacc tgcttaggtg accagcgatt acggacaaat tcttttgctc
     2401 ttgccccgag attaagccga tattcaggat cgacaatcat tttttcgatg gcggcttccg
     2461 cgctatttgg ggcgcaaaat aggcttgggg gaacatcttg ctcagacaat acttggccga
     2521 tatgctctgc gtagtaccct cccaccactg ccggttttcc gtaaaaggct gcttcaaccg
     2581 caaacagggc caggggcatg tctgaataaa gttcgtccac gacaaaatca caattttgaa
     2641 ttgcctctat gacttcccga ttcggacgtc caataatttc tatccactcg atctggtgtc
     2701 ctttttttcg cagcgattcg ataatttgac gaaacactac agtacctttc ggaccgggtt
     2761 ttgacggagc atgca
//
LOCUS       DAOMXO010000298         2763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20888_length_2763_cov_4.212703, whole genome shotgun sequence.
ACCESSION   DAOMXO010000298 DAOMXO010000000
VERSION     DAOMXO010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20888_length_2763_cov_4.212703"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLH57_06915"
     CDS             <1..491
                     /locus_tag="PLH57_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02382.1"
                     /translation="MRLFKHFRMVAMFLVLANLLATSVLAKDLRSRLGIGFVDQYSNG
                     VPALSAKYGLTKDLHIATALGFSTADRADVVFSGKIFKNIFFETNLNFYSALALAYLK
                     HDERSGIEILGVLGAEFFIPGVDSLGLSFETGVSGSNITNTFVLKTIGFTFLHAGMHF
                     YF"
     gene            526..1305
                     /locus_tag="PLH57_06920"
     CDS             526..1305
                     /locus_tag="PLH57_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02383.1"
                     /translation="MSAWLLLGLLSPTLAKAVDEYEAVGAAKRAAAPDDEPTSDGPIS
                     GPFSEYGEFDAAEDEANDEKFFQFGRFFGVGLGTGLTTATGNSGKLYQGGFPMIDGRL
                     AYWFDFQVALQVALQNSKHNYNLPVSSGNSELVDTNLFRILFQVKYYFDTRDLSAPIT
                     FVGPHMIVGGGMYSRSDNKTAVDSQGTSGATSTEKVNAFGMNFGMGFELTIKPRKTYL
                     QLEALAHLVQFSDQYDNRYAPTVNDKTGTWITAGIALMWTW"
     gene            complement(1330..>2763)
                     /gene="der"
                     /locus_tag="PLH57_06925"
     CDS             complement(1330..>2763)
                     /gene="der"
                     /locus_tag="PLH57_06925"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRK02384.1"
                     /translation="VGGALWVRRSFFMKKVCLVGRPNVGKSSLFNRLIGRRKALVLDQ
                     PGVTRDVVLAELELDAGTVQLADLAGLESDLSRRDRSPRAELARLAQEAALDYLKKSD
                     LVLFVVDARSPLTPHDEHLSTLVRMSGKSVIMVLSKVEGKVEDLARMETAALGWEHVL
                     CTSAEHNLGISDLKDAIAGALELSSDEPNETLEESVAKTPEARGLTKERPIRMAVYGR
                     PNVGKSTLVNYLLGEKRMITSPIAGTTVDTVDSDFEKDGRYYRILDTAGIRRKSKTDA
                     GVEVLSVVKAIRSVVDVDIALFLIDGYEGITDQDEKIAGELRDSGRPIVLIVNKWDLC
                     GVTKEDYAKRLRDNLDFLDYAPILFICAEKGEGIEPLFDLIDEMVRARQFVAPTGELN
                     RFIEMVEGCNVAGDAKLYYASQTQVSPPTITFLVNDPKNVRFSYERYLRNELRNRYNW
                     MGSPIRLVFKARSRSPSKKSRGSSAQR"
BASE COUNT          666 a          678 c          674 g          745 t
ORIGIN      
        1 gtatgagact tttcaagcat tttcgaatgg ttgcgatgtt tttggtgctt gccaatttgc
       61 tcgccacgag tgttttagcc aaggatttac ggagtcgact cgggatcgga tttgttgatc
      121 aatattccaa tggcgtaccg gcactgagcg ccaagtatgg actaaccaaa gatcttcaca
      181 ttgcaacggc gctcgggttc tcaaccgccg accgagccga tgtggttttt tcgggtaaaa
      241 tttttaagaa tatttttttt gaaaccaatc tcaattttta cagcgcgctc gccctggcct
      301 atctaaagca tgacgaacgt tcgggaatag aaattctcgg ggtgctcggg gccgagtttt
      361 tcatacccgg agtcgacagc cttgggcttt ccttcgaaac aggagtgagt ggctcgaata
      421 ttacgaacac gtttgttctt aaaacaattg gatttacctt cttgcacgcg gggatgcatt
      481 tttattttta agccaatact gcacagatca ttcgggactt gcctggtgag cgcttggctg
      541 ttgctgggtt tgctgtcccc gactctcgcg aaggctgttg atgaatacga agcagtaggt
      601 gcagccaaac gcgcagctgc tcctgacgac gaacccacta gtgatggccc aatatcgggg
      661 ccgttttcgg agtatggcga attcgacgcc gccgaagatg aagcaaatga cgaaaaattt
      721 tttcagttcg gtcgcttttt cggcgttggt ttgggcacag gactcacaac tgcaactgga
      781 aactcaggca agctttacca gggcggattt ccgatgatcg acgggcgtct ggcctattgg
      841 ttcgattttc aggtcgctct ccaggttgcg ctgcagaaca gtaaacacaa ttacaatctc
      901 cccgtaagta gcggtaactc ggaattggtg gacaccaatc tttttcgaat tttgtttcag
      961 gtaaaatact acttcgacac tcgcgatcta tcggctccaa ttacatttgt agggcctcat
     1021 atgatcgttg gtggtggaat gtactctcgg tccgacaata aaactgcggt agactcccag
     1081 ggtaccagcg gcgcgacgtc gaccgaaaag gtgaatgctt ttggcatgaa cttcggtatg
     1141 ggttttgaac tcacgattaa acctagaaaa acctacttgc aactcgaggc gttggctcac
     1201 ttagtgcagt tctccgatca atacgataat cgctatgctc ccactgtgaa tgataaaaca
     1261 ggaacttgga ttaccgcggg catcgcactg atgtggactt ggtagtctaa ctcacgaact
     1321 gaatcgcact tagcgctgcg cggaagaacc acgcgatttt tttgaaggac tgcgagatct
     1381 agccttaaag actaatcgta tcgggcttcc catccagtta tagcgatttc gaagttcgtt
     1441 tcgaagataa cgttcgtagg aaaaacgtac atttttgggg tcgtttacta aaaatgtaat
     1501 ggtaggtggg cttacctggg tttgacttgc gtaatacagc tttgcatcgc cggctacatt
     1561 gcatccttcg accatttcga taaaacgatt gagttcgccc gtgggtgcga cgaattgccg
     1621 tgcgcgtacc atttcatcga tcaaatcaaa gagcggttca atcccctcgc ccttttccgc
     1681 gcagataaac aaaatcggtg cataatctag aaaatctaaa ttatctcgca aacgcttggc
     1741 gtagtcttcc ttggttacgc cacagaggtc ccatttgttg acgatcaaca ctatgggacg
     1801 gcccgaatcg cgcaattcgc cggcgatttt ttcgtcttga tcggtaatgc cttcgtaccc
     1861 atcaatcaaa aacaaagcga tatcgacgtc gaccacagaa cggattgctt ttacaacgct
     1921 aagtacttct acacctgcgt cggtcttgct tttacggcga atgcctgcgg tgtctaaaat
     1981 gcggtaatag cgaccgtctt tttcgaaatc actgtcgacc gtatctactg tagtgccagc
     2041 gattggcgag gtgatcattc gtttttcgcc cagtaaataa tttacaagtg tgcttttccc
     2101 tacgtttggg cggccataca ctgccatgcg aatagggcgc tctttcgtga gtcccctagc
     2161 ttccggagtt ttggcgactg attcctcgag cgtttcgttg ggctcatcac tagataattc
     2221 gagcgctccg gcgatggcat ctttaagatc agaaattcca agattatgct cggccgaagt
     2281 gcaaagcaca tgttcccagc cgagagccgc agtttccata cgagcgagat cttcgacttt
     2341 accttctact ttcgatagca ccatgatcac cgattttccg gacattcgaa ctagcgtcga
     2401 aaggtgttcg tcgtggggag tgagcggcga tcgagcatct acaacgaaca aaacaagatc
     2461 cgattttttt aggtaatcaa gtgcggcttc ttgtgcaagg cgagcaagct ctgcgcgtgg
     2521 tgaacggtct cttcgcgaga gatccgactc gagccccgcc agatccgcca gctgcacagt
     2581 gcccgcatct aactccagtt ccgctagcac gacgtctcgg gttactcctg gctgatcaag
     2641 gacaagcgct tttctgcggc cgatcagacg attaaaaaga gaacttttcc caacgttggg
     2701 tcggccgacg agacagactt ttttcataaa gaaacttctc cgtacccaaa gcgctccacc
     2761 aac
//
LOCUS       DAOMXO010000299         2755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_20973_length_2755_cov_4.362963, whole genome shotgun sequence.
ACCESSION   DAOMXO010000299 DAOMXO010000000
VERSION     DAOMXO010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20973_length_2755_cov_4.362963"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLH57_06930"
     CDS             <1..448
                     /locus_tag="PLH57_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRK02385.1"
                     /translation="DSFATAFAGAFGTSSGTAYIESAAGIEMGGRTGLTAVFTALCFL
                     PCFFIAPIAGMVPAYATASVLILVGALMFRSITKIRFERLEDLVPSFLTIVMIPLTFS
                     ITQGILWGFILHVTAYTLAGRSREIKAQMWVIAVFAIGLLVLDHFS"
     gene            complement(425..1438)
                     /locus_tag="PLH57_06935"
     CDS             complement(425..1438)
                     /locus_tag="PLH57_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02386.1"
                     /translation="MTSNTSDALPISGIGSLPHHNVDAALEYSFRFDIPFFPQIPLRN
                     PNEYMLPQALHGLPGLVNDSEGSVTLDLLAWKSGKDVFSDLLDQGLRNGITKPFELST
                     EYASCWKPFLWEIIERKQPLVKIQIAGPLTCQWALRMSDQTPVFANPDVASQVLKLVT
                     LRAQSMIETIQKTGAHTIIYLDEPALFALDVKNPKHILALGELRLLIGALKKMGATVG
                     LHCCSNTDWKAVLSLDLDILSLDVALSLDGLTQHHRELDQFVADGRRLSLGIVSTNEN
                     SESTSADQIAGILRKLPPRALSQALLTPACGLAFKAAQECEHIYTVLREYQTTLLRKV
                     IQN"
     gene            complement(1435..>2755)
                     /locus_tag="PLH57_06940"
     CDS             complement(1435..>2755)
                     /locus_tag="PLH57_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRK02387.1"
                     /translation="KGDVHDIGKNLVEIVLANNGFQVVNLGIKVPPEQLISAVKEHSP
                     NILGLSGLLVKSAQQMVVTAEDLAKAGIDVPILVGGAALSRRYVDRSIAPAYGGTVAY
                     AKDAMNGLDLAKQIVEPSSFERLKKTLSDERSISEANTPTETKLNPISDRRSKKVEVL
                     TDFPEPTDFHRHVLKNSPIDQIWSYINPLMLYGRHLGIKGGVVRQLVAKEFTNIPTEQ
                     KKAVEIWNAVEEVKLYCKERELLKPSAVYRFYKAGSEGNSIFLFDDGKQVADFEFSRQ
                     PGEDGLCLSDYLAPFKGGQVPDNIAMFVVSSGSGVRTQAEIWKNSGDYLKSHIIQALA
                     LETAEAYAEFLHSRIRAMWGFADPIDMTMMERFQAKYRGKRYSFGYPACPRLNDQEKL
                     FSLLKPQEIGVELTDGFMMEPEASVSAIVFHHPQASYFSVGEGGENR"
BASE COUNT          711 a          630 c          639 g          775 t
ORIGIN      
        1 ggacagcttt gccaccgcat ttgcgggcgc gttcggtact tcctcaggaa ctgcatacat
       61 cgaaagtgcc gctggaattg aaatgggagg tcgcaccgga ttgaccgccg tttttactgc
      121 actttgcttt ttaccatgtt tttttattgc ccccattgca gggatggttc cagcttatgc
      181 gacggcttcg gtactgattt tagtgggtgc gttgatgttt cgttctatca ctaagattcg
      241 ttttgaacgt ctagaagatt tggttccgtc ttttcttacc attgtcatga ttccgctaac
      301 cttctcgata actcagggaa tactctgggg atttatttta catgtgaccg cctatacgtt
      361 ggccggaaga agcagagaaa ttaaggcaca aatgtgggtg attgcggttt tcgcgattgg
      421 tcttttagtt ttggatcact tttcttaaca acgttgtctg gtactcgcgc aacaccgtat
      481 aaatgtgttc acattcctgg gcggctttaa atgcgagccc acaggcggga gtgagcaagg
      541 cttgagacag tgccctcgga ggtagttttc tcaagatgcc tgctatttga tccgcagaag
      601 tggattcgct attttcattg gtggatacga taccaagcga aagtcttcgt ccgtctgcga
      661 cgaattgatc caactcacgg tgatgttgag taagtccgtc gagtgaaaga gccacatcca
      721 acgataagat atcaagatcg agcgacaaca ctgctttcca atcagtatta ctgcaacagt
      781 gtaatccgac agtcgctccc atctttttta atgctccaat taaaagccgg agttccccca
      841 atgccaaaat atgttttggg ttcttaacat ccagagcgaa gagtgcgggt tcgtctaaat
      901 aaataatggt atgcgcacca gttttttgaa tggtttcgat catcgattgg gcgcgcaaag
      961 taacaagctt taagacttga gaggctacgt ctggattggc gaatacagga gtctgatcac
     1021 tcatgcgcag cgcccactga caagtaagag ggccggcgat ttgtattttt acgagcggtt
     1081 gtttacgctc gatgatttcc caaagaaagg gtttccaaca ggaggcgtat tcggtggata
     1141 actcaaatgg ttttgtaatc ccgttacgca aaccttgatc caaaagatca ctaaaaacgt
     1201 ccttgccgga tttccaggca agaagatcaa gagtaactga gccctcagaa tcatttacta
     1261 accccggcag cccatgcaac gcctgaggta acatatactc attcggattc cgtagtggaa
     1321 tttgtggaaa gaaaggaatg tcgaatcgaa aactatattc aagtgccgca tcgacgttgt
     1381 ggtgaggtaa acttccaatt ccgctaatgg gcagggcgtc ggaagtattc gaagtcatcg
     1441 attttctccg ccttcgccaa ccgaaaaata acttgcttga ggatggtgaa acactatcgc
     1501 cgaaacgctg gcttcgggtt ccatcatgaa tccgtcagta agttctacgc cgatttcttg
     1561 cggcttcaga agcgagaata gcttttcctg atcattcaaa cgcggacacg cgggataacc
     1621 gaaagaataa cgctttccac gatatttggc ctgaaaccgc tccatcatgg tcatgtcgat
     1681 aggatccgca aaaccccaca ttgcgcgaat gcgagaatgt aaaaactccg cgtaagcctc
     1741 ggcggtttct agcgccaaag cctgaatgat gtgtgatttt aaatagtcgc cactgttttt
     1801 ccagatttcg gcttgagtgc gcacgccaga tcccgaagat actacaaaca tggctatgtt
     1861 gtctggtact tgtccaccct taaacggcgc caggtagtca ctgaggcaca gaccatcttc
     1921 tccgggttgt ctggaaaact caaaatcggc aacttgtttt ccgtcatcga aaagaaagat
     1981 ggagttcccc tcagaaccag ctttgtaaaa gcgatatact gccgatggtt taagcagctc
     2041 tcgctcttta caataaagtt ttacttcttc gacggcgttc catatttcga cggccttttt
     2101 ttgttccgtg ggaatattgg taaattcctt agccaccaac tgacgaacga cgccaccttt
     2161 tatgcccaaa tgacgaccgt aaagcataag tgggttaata taggaccaaa tctggtcaat
     2221 cggtgaattt ttaagaacgt gacgatgaaa atcggtgggt tccggaaaat cagttagcac
     2281 ttcgactttt ttggaccgcc gatcgctaat ggggttcagt ttagtttctg taggggtatt
     2341 tgcttcgcta atactacgtt cgtcgcttag cgttttcttt aggcgttcaa acgaagaagg
     2401 ctcaacaatt tgcttcgcca gatctagacc attcatggca tctttcgcat aggctacggt
     2461 acccccgtag gctggagcga ttgatcgatc tacatatcgt ctagaaaggg ccgctcctcc
     2521 gactaaaatt ggaacatcta ttccagcctt tgctaaatct tcagcggtaa ctaccatctg
     2581 ttgagcagac ttaactaaca gaccggaaag accaagaatg ttgggtgaat gttcttttac
     2641 cgcagaaatt aattgttccg gaggaacttt gattcccagg tttacaactt gaaacccatt
     2701 attcgcgagt acaatttcga ctaagttctt tccgatatcg tgaacgtcac ctttg
//
LOCUS       DAOMXO010000300         2743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21079_length_2743_cov_5.120164, whole genome shotgun sequence.
ACCESSION   DAOMXO010000300 DAOMXO010000000
VERSION     DAOMXO010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21079_length_2743_cov_5.120164"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLH57_06945"
     CDS             complement(<1..596)
                     /locus_tag="PLH57_06945"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRK02388.1"
                     /translation="MKPVADIATGLTFDDVLLVPAYSEVLPSEVNVATRLTRKIKLNI
                     PLVSAAMDTVTESRAAIVMAQEGGLGVIHKNMTPDEQALEVERVKKSEWGMILNPVSI
                     APNVRLREAIELMQRTKISGLPVTVKGKLVGILTHRDIRFEENLNLLVKDLMTKDGLV
                     TAVEGVTLEAAKKLLHKHRIEKLPVVDRQGMLRGLITI"
     gene            complement(593..1135)
                     /locus_tag="PLH57_06950"
     CDS             complement(593..1135)
                     /locus_tag="PLH57_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007547159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRK02389.1"
                     /translation="MLHSFRGVSPKLHSSVFVTPSSDIIGDVEIGENSSVWFNVVIRG
                     DVNTVRIGRDTNVQDGSILHVTHKTAALKIGNGVTVGHSVTLHGCTIKDFVLVGMRAV
                     VLDGAVVGEESVIGAGALVTQNMVIPPRSMVLGSPGKVVRPLRDEEIAFLHQSPENYK
                     QYVAWYREDGPWAATKGSEK"
     gene            complement(1217..1825)
                     /locus_tag="PLH57_06955"
     CDS             complement(1217..1825)
                     /locus_tag="PLH57_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02390.1"
                     /translation="MKGIWNQFFQVRTLGAVVLLLATAGVSGCPSDPGAEPAPTQRFR
                     AALTTTNSAISLRRYIYEGNHLLVEAIERAPLSGELVVRVEPSPTNAADSTTRLFVEQ
                     IKMQIGSTGVFRDLRLRLGIQVEQDGFSDLLFDGSAKVGEVAWPLPTKKAEDLLFEVP
                     NGADTQEFILRIAPVDWVPLFEASNESLSFAAFEFVGELEPF"
     gene            complement(1791..>2743)
                     /locus_tag="PLH57_06960"
     CDS             complement(1791..>2743)
                     /locus_tag="PLH57_06960"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRK02391.1"
                     /translation="TDTKVFHQSASAFVNIMKGCDKFCSFCIVPFTRGREKSRPIAEL
                     VADVQRLVLLGVREVTLLGQNVNSFGKGNNETFPQLLRALDSVAELKRLRFTSSHPID
                     FSDDLINCYGSVRALCNQLHLPVQSGSNAVLEKMHRHYKIETYYEQIRKWRERCPDGA
                     LSTDMIIGFPTETDADFELTMKLLEDLRFDIVYAFAYSPRPGTKAAKLQDDVPNEVKH
                     ERLLRFQRRAVEIANENNQRLVGKSMEVLVEGRTKALKTSKSQNMFMGRSRCNKVVNF
                     AFEGARDLKGKFVQVRIERATGLALTGVVDEGDLEPVLSS"
BASE COUNT          683 a          752 c          635 g          673 t
ORIGIN      
        1 ttgattgtga tcaatccccg caacattccc tggcgatcaa cgactggaag cttttcgatt
       61 cggtgtttat gaagcaattt ttttgctgcc tcaagcgtaa cgccttcgac tgcggttacc
      121 aaaccgtctt tggtcatgag atctttcact aacaagttga gattttcttc aaagcgaatg
      181 tcacgatgag ttagtattcc aacgagtttg ccctttacgg tgaccggcaa accagaaatt
      241 ttcgtacgct gcattaactc aatcgcttca cgcaaccgca cattcggagc aatagaaacg
      301 gggttgagaa tcatgcccca ctccgatttt tttactcgtt ccacttcgag tgcctgttcg
      361 tcgggggtca tgtttttatg aataacgcca agtccgcctt cttgcgccat cacgatggcc
      421 gcacggcttt cggtgacggt gtccatcgct gccgatacca gcggaatatt aagtttaatc
      481 ttgcgggtta accgagtggc aacgttgacc tcagagggta aaacctccga ataggctgga
      541 acaagcaaca cgtcatcaaa cgtgagcccc gttgcaatat ctgccaccgg tttcattttt
      601 cgctcccttt ggtcgcagcc cacggcccgt cttcacggta ccaagccacg tattgtttgt
      661 agttttcagg actctggtgc aagaacgcga tttcttcgtc gcgcagaggt cgcaccactt
      721 ttccgggact tccaagcacc atcgaacgcg gcggaatcac catgttttgc gtcactaggg
      781 cacccgcacc aatgacactt tcctcaccaa ccacagcacc atctaaaacc acggcccgca
      841 tgccgaccaa cacaaagtct ttaatggtac aaccgtgcaa ggtaacacta tgccctaccg
      901 tcacgccatt tcctatttta agagcggccg tcttatgggt gacatggagt atgctgccgt
      961 cctgaacatt ggtgtcacgc ccaatgcgaa cagtgttcac atcaccgcga atcacaacgt
     1021 tgaaccagac cgacgaattt tcgccgattt caacgtcgcc aatgatgtcg gaactaggtg
     1081 taacaaaaac actggaatgt aattttggcg aaacgccacg aaaggaatga agcatacgaa
     1141 taattacaat gcagtgtctt tgatcggcaa caaaaatacg gccgaacctt cgtttttacg
     1201 caaagcatca cgaagattaa aagggctcga gctcgccgac aaactcgaat gcagcaaacg
     1261 agagactctc gttactggcc tcgaataacg gtacccaatc gaccggggca attctaagaa
     1321 taaactcttg cgtgtctgcc ccgttgggca cctcgaaaag taagtcttcc gcttttttgg
     1381 ttggtagcgg ccaagccacc tctcctacct ttgcggaacc atcgaaaagt aaatcggaaa
     1441 agccgtcttg ttcgacctga attcctaatc gcaatcgaag gtcccggaac actcccgtcg
     1501 aaccaatctg cattttgatt tgttccacga agagtcgcgt tgtcgagtcg gcagcattcg
     1561 ttggtgaagg ctccacccgt accacgagtt caccggaaag aggcgcgcgc tcgatagcct
     1621 caacgagcaa atggttgcct tcgtaaatgt atcgtcgaag cgaaatcgca ctattggtgg
     1681 tcgttagcgc agcccgaaat cgctgcgtag gcgctggctc agcaccgggg tccgaagggc
     1741 agccgctcac gcctgccgtg gcaagcaaaa gtacaactgc accaagcgtc ctaacttgaa
     1801 agaactggtt ccaaatcccc ttcatcgaca acccccgtta acgctagacc cgtagcccgt
     1861 tcgattcgaa cttgaacaaa tttccctttt aaatctcgcg cgccttcgaa cgcaaaatta
     1921 actactttat tgcaacgact ccgacccatg aacatgtttt gcgacttcga tgttttaagt
     1981 gctttggtgc gcccctctac taagacttcc atcgacttgc cgacgaggcg ctgattgttt
     2041 tcgttggcaa tctcgactgc acggcgttga aagcgcaaca atcgctcatg tttcacttca
     2101 ttcggaacat catcttgcag ttttgccgct ttggttccag ggcgcgggct gtaagcgaac
     2161 gcgtagacaa tatcgaaacg cagatcttcg agaagcttca tcgtaagttc aaaatcggcg
     2221 tcggtctcag tgggaaatcc aatgatcatg tcggtagaca acgcgccgtc tgggcagcgc
     2281 tcgcgccatt ttcgaatctg ctcgtaatag gtttcgattt tgtagtgccg atgcattttt
     2341 tccagcactg cgttggatcc actctgaacg ggcagatgca actgattgca aagggctcgc
     2401 acactgccgt aacagttgat tagatcgtcg ctaaagtcga tcgggtgcga actggtaaat
     2461 cgcaagcgtt taagctcggc gacactatcg agcgcgcgca aaagctgcgg aaacgtttcg
     2521 ttgttgcctt taccgaacga attcacgttc tggccaagca gcgtgacttc gcgcacgcca
     2581 agcaacacca aacgctgcac atctgcgact aactccgcaa tcgggcgact tttttcgcga
     2641 cccctcgtaa acggcactat gcagaaacta caaaacttat cgcagccttt cataatgttg
     2701 acgaacgccg atgcggactg atgaaacact tttgtgtcgg tcg
//
LOCUS       DAOMXO010000301         2740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21118_length_2740_cov_3.879702, whole genome shotgun sequence.
ACCESSION   DAOMXO010000301 DAOMXO010000000
VERSION     DAOMXO010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21118_length_2740_cov_3.879702"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLH57_06965"
     CDS             <1..289
                     /locus_tag="PLH57_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02392.1"
                     /translation="STNWPTPCTTSTLRPLAIKRTVNQGRILTLGVKKKYRALGLGSL
                     MFLKYYETAPSMGYPTAEASWILEDNAAMNQGLKMMNADLYKTYRIYDKTL"
     gene            316..840
                     /gene="ruvC"
                     /locus_tag="PLH57_06970"
     CDS             316..840
                     /gene="ruvC"
                     /locus_tag="PLH57_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRK02393.1"
                     /translation="MTDPIKSAIRVLGIDPGSRLMGFGMVSKNGTKLTTSGYGTLKFN
                     TKVEANERLIEIYDGVRALLEKFKPDHVAIEKVFFAKNVVSALKLGQARGVAVLAVAQ
                     AGIPIYEYSPNEIKLSTVGFGHADKIQVAKMVRLLLGVENFESADSSDALAIAICHLQ
                     THQFRRNLPRGAEL"
     gene            837..1541
                     /gene="ruvA"
                     /locus_tag="PLH57_06975"
     CDS             837..1541
                     /gene="ruvA"
                     /locus_tag="PLH57_06975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRK02394.1"
                     /translation="MIGYLRGQILSKDGSDLVIDVQGVGYALSVPQRMSVKVNSTSEF
                     FVHTHVRENAIDLFGFATGWERQVFLLLISVSGIGPRTALGLLSQLEPVQLLTALVRE
                     DRATLSSVSGIGKKTAERLIVEIADKGRKLLTERPGGVSNQTTVAAETLPSSAKPVRT
                     KVPAQGASRNSDREIEITDIWNDALVALVNFGYREGDALAVIREASAEREAEGLEVQL
                     EPLLKRCLQIVSKGRA"
     gene            1542..2564
                     /gene="ruvB"
                     /locus_tag="PLH57_06980"
     CDS             1542..2564
                     /gene="ruvB"
                     /locus_tag="PLH57_06980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRK02395.1"
                     /translation="MELDVHDKSQADDEATVDVALRPQDFSEYIGQAKVTANLLMYIE
                     AARKRGDSLDHCLFSGPPGLGKTTLAHIVAKHMGSQLHTVAAPAIDKKGDLAAILSSL
                     NKHDVLFIDEIHRLPRVIEEVLYSAMEDFKLDVIIGQGPSARTLRLDLPRFTLVGATT
                     RAGLLTNPLRDRFGVSFRLDFYSPKELQSIVERSSRILTVPIDAEGAQEIAKRSRGTP
                     RIANRLLRRVRDFAQVRGDGTVDRAVACKGLELLEVDHQGLDSMDRRLLSLIVEKFDG
                     GPVGIETLSAAIGEERGTIEEIYEPFLLQEGFLQRTPRGRLATRKAFELLGLTYSAND
                     PTPTLF"
BASE COUNT          673 a          643 c          734 g          690 t
ORIGIN      
        1 gtcgacgaac tggccgaccc cctgcacgac ctcgaccttg cgccccttgg ccattaaacg
       61 aactgtaaat caggggcgca tattaacgct cggagtaaaa aagaagtatc gggctcttgg
      121 tcttgggtcc ttgatgtttc tcaaatacta cgaaacggcg ccaagcatgg gttacccgac
      181 ggccgaggct tcgtggatct tggaagacaa tgcggcaatg aatcaggggc tcaaaatgat
      241 gaatgccgat ctatacaaga cctatcgcat ctacgacaaa acgttgtaat ctcgtcctag
      301 agggcgaaat tcgcgatgac tgatcctata aaatcagcga ttcgggtgct cggaatcgac
      361 cccggctcac gcttaatggg ttttggcatg gtttcgaaaa acggaaccaa gctgaccact
      421 tcgggttatg gcacgctcaa gttcaacacc aaagtcgaag ccaacgaacg cctgatcgaa
      481 atttatgatg gtgttcgggc tttacttgaa aagttcaaac ccgatcacgt ggcgattgaa
      541 aaggtgtttt tcgccaaaaa cgttgtctcc gccttgaagc tgggtcaggc ccgtggggtc
      601 gcagtgcttg cggttgcgca ggccggtatt cctatttacg aatacagtcc caacgaaatc
      661 aaactctcca ccgtgggttt tggtcatgcc gataaaattc aagtcgccaa gatggtacgt
      721 ttacttcttg gagtggaaaa ttttgaaagt gctgatagct ccgatgcgct tgcaatcgct
      781 atttgccatt tacagacgca tcagttccgt aggaatttac cccggggtgc agagttatga
      841 ttggttatct acgcgggcaa attctttcta aagacgggtc tgatttggta atcgacgtgc
      901 aaggtgttgg ttatgcgttg agcgttcctc agcgaatgag cgtaaaggtt aactcgacgt
      961 cggaattttt cgttcacact catgtgagag aaaacgcaat tgatcttttt ggattcgcca
     1021 ccgggtggga acgccaagtt tttctgctat taatcagcgt ttcgggcatt ggcccgcgga
     1081 ctgcactggg cttattaagt cagctcgagc cggtgcaact tttaacggct ctcgtacgtg
     1141 aagatcgggc aacactctcg agtgtgtcag gtattggcaa gaaaaccgcc gaacgcttaa
     1201 ttgtggaaat tgcagataag gggcgcaagc ttttgacgga acgtcctggt ggcgtctcga
     1261 atcaaacaac agttgcagcc gagactttgc cgtctagtgc gaaacctgtt cgaaccaagg
     1321 tgccggctca gggggcttcg cgcaattcgg atcgcgagat tgagatcaca gacatttgga
     1381 atgacgcact cgttgcgctc gtgaactttg gttaccgcga aggagacgct ttggcggtga
     1441 ttcgcgaagc ctcagcggag cgagaggcgg aaggtctaga agtgcaactc gagccgttgc
     1501 ttaaacgctg tttgcagatt gtctcgaaag ggcgggccta aatggaactt gatgtacacg
     1561 acaaatccca ggccgatgac gaagcgacgg tcgacgtagc tctgcgaccg caagatttca
     1621 gtgaatacat cggccaagca aaagtgacag cgaatttgtt aatgtacatc gaagctgcac
     1681 gaaagcgcgg tgattcgctc gaccattgtt tgttttcggg ccctccgggt ctcggtaaaa
     1741 cgactcttgc gcacattgtc gccaaacaca tggggtcaca gctccacacg gttgcagccc
     1801 ctgcaattga caaaaaaggt gacctagcag ccattctttc gagcctcaat aaacacgacg
     1861 tactttttat cgacgaaatt catcgattgc cgcgtgtaat cgaagaagtg ctctacagtg
     1921 cgatggagga ctttaaactc gacgtcatta ttggtcaggg accgtcggcc cgtacgctgc
     1981 gactagattt gcctcgtttt actttagtgg gcgctacgac ccgtgcgggt ttgctgacga
     2041 atcctttgag ggaccgattt ggcgtttcat ttaggctcga tttctattcg ccaaaagagc
     2101 tgcaatcaat tgttgagcgg tcttcgcgca ttctaacggt accgatcgac gccgaaggtg
     2161 cgcaagagat agcaaaacga agtcgcggta ccccgcgtat agccaatcgt ctattaagac
     2221 gtgtgcggga tttcgcccag gtgcggggtg atggtacggt tgatcgtgcg gtggcttgca
     2281 agggactcga gctcctagag gtcgaccacc aggggctcga ttcaatggat cgaagacttc
     2341 tttctctgat tgttgaaaaa ttcgatggcg gacccgttgg aattgaaacc ttgtcggccg
     2401 ccattggtga agagcgcgga accatcgaag aaatttatga gccgtttttg ctccaagagg
     2461 ggtttctcca gcgcacaccg cgtgggcgac tcgccactcg aaaggcgttt gaactgctgg
     2521 gtttgaccta ttccgcgaat gaccccacgc cgaccttgtt ctgaggttca agattgaagc
     2581 ttctgttgca aaaagcacac accgcttaac aattttgtgc ggtgaatgac acgaaaactc
     2641 agttaaaatg acgcggattg tctttttgaa tttgcataag catattattt tgttaagggt
     2701 taatctgtta taaatactcc actattgtgg cataagtgtt
//
LOCUS       DAOMXO010000302         2727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21264_length_2727_cov_3.915419, whole genome shotgun sequence.
ACCESSION   DAOMXO010000302 DAOMXO010000000
VERSION     DAOMXO010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21264_length_2727_cov_3.915419"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..608)
                     /locus_tag="PLH57_06985"
     CDS             complement(174..608)
                     /locus_tag="PLH57_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02396.1"
                     /translation="MSEANLKILAGSALVFYSAAMRIDKVPVRLVKSGSSTSTAGEIE
                     IQYWEKRERTLRALKFTGLCWAAAFISIALPIVHFVLVPAFLLLGPIVGLGVLNRESQ
                     ILRGSGLCPHCQAPFVIAKAPNQLPLSDVCTSCRAEVLVEQT"
     gene            complement(645..719)
                     /locus_tag="PLH57_06990"
     tRNA            complement(645..719)
                     /locus_tag="PLH57_06990"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(684..686),aa:Arg,seq:cct)
     gene            902..1921
                     /locus_tag="PLH57_06995"
     CDS             902..1921
                     /locus_tag="PLH57_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRK02397.1"
                     /translation="MSARILLGFLVAIAGVDRAAAGPLRSSVPTCAEVILNSAYGADS
                     SKKLLTRQDALDLVRLYKTGSERERNIVILKLRNVLFGRAMNEWRRFYGMDLEEFQSE
                     AILGLVQGLNGFDENVSQDVLAYLIRAMDNTLRRFVQKNLKPVRFVSTKAEEILFGHY
                     FRIVHEIRASGKEVTAESVCAQMLRYGKKNKNKNILSVNWKNKLVIENVRSWMQVINV
                     RTNYLVNSEDQIDSDVMASTNQVAVEIAETEKQRIERESSSQFFRRMIEPMVKGLSVR
                     EKQILERRILCEEGCESTLQELANEWGGVTRERARQLDVRVRRKLDTFIKDTPAAKRL
                     LRQFP"
     gene            complement(1930..>2727)
                     /locus_tag="PLH57_07000"
     CDS             complement(1930..>2727)
                     /locus_tag="PLH57_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02398.1"
                     /translation="KSQPEFGDWNTNVRCKGDKCASPKKDSVVSVEHLSSHFSHPGGI
                     QSFGSIVAVGIDINNNNKAGSEVRFYDYADPTSPVERRDWRILRTNVRAEAVAMSQIK
                     RGVHAGKYVVAVGSHQLSHLTFYISNDSDLNASGFRFQEQATWTERGNFDIPNYQGMQ
                     LISECSGQMYLVGTYKSKPLFGLPWGGKDWIDTYKIELSPYAGKAPQIAKFKNKHMTL
                     KKSFFSAGGAVYVEPGTGELKAYATDYWPLNPFKRDTAFRFEEFAPK"
BASE COUNT          740 a          617 c          689 g          681 t
ORIGIN      
        1 taggaataag gcgctcttca tttctgcgcc aggaggattc gaggtgttga tttgaatgta
       61 tttctggaga acggttatta cttcttggta aaaagccggc gtgggcgtct gagcgccctg
      121 cgacggaaaa gaaatgaaca gtatcagcca gacgaacgcc atgtttagaa gtttcatgtt
      181 tgttcgacca gaacctctgc gcgacaagaa gtgcaaacat cgctgagcgg tagttgattc
      241 ggcgccttcg cgattacaaa cggagcttgg cagtgggggc ataggccgct accgcgaaga
      301 atctgggatt cacgatttaa aacgcctaat cccacaatgg gacccagcag taaaaaggcg
      361 ggaactaaaa caaaatgaac gatcggaagt gcaatggaga taaacgctgc ggcccaacat
      421 aggcccgtaa acttcagtgc ccgtaaggta cgttctcgtt tttcccaata ctgaatttcg
      481 atttctccag ccgtacttgt ggagctaccc gacttaacga ggcgaacagg cactttatcg
      541 atacgcattg ctgcactata gaatacgagc gcagagccag ccagaatttt caaattagcc
      601 tcagacaaag aagatgattc cgcatcaggc taatttcaaa attctggtgg tcccaccaag
      661 aatcgaactt ggaactgagc tttaggaaag ctccgttata tccatttaac tatgggaccc
      721 cgccgctctc aattatcgac cttgcgttcc gatttcacta agaaatttca ttcggcggac
      781 agtgttttct cggtacaaaa gtcattagat ctatatatga ctaaaaaagt atggaataaa
      841 ataacgcgct atgttatcgc tatttcattc aaaaacggtt caaaatccag aggaatctat
      901 agtgagcgca cgcattttat tgggctttct agtggccatc gcaggcgtcg atcgagcggc
      961 agccggcccg ctgcggtcca gcgtccctac atgtgccgaa gtaatattga attcggctta
     1021 tggcgctgat tcctcaaaaa agctactaac gcgccaagat gccctggatc tggtgcgcct
     1081 ctacaaaacg ggcagtgagc gcgaacgtaa tattgtgatc ttaaagctta ggaacgtcct
     1141 ctttgggcga gccatgaacg agtggcgccg tttttacgga atggacctag aagaatttca
     1201 atccgaagcg atcctgggcc tagttcaggg cctcaacggt ttcgacgaaa acgtttcaca
     1261 agatgttttg gcctatctaa ttcgtgcgat ggacaacacg ttgcgacgat tcgtgcaaaa
     1321 aaatcttaaa ccggtgagat tcgtctcgac gaaagccgaa gaaatactct ttggtcatta
     1381 ttttcgaatc gttcacgaaa ttcgcgcctc tggcaaagag gtcacggcgg aaagtgtttg
     1441 tgcacagatg cttcgctacg gaaaaaagaa taaaaataaa aacattcttt cggtgaattg
     1501 gaaaaacaaa ctagtgattg aaaacgttag aagctggatg caggtaataa acgtgagaac
     1561 gaattacctc gtaaattcag aggatcaaat tgatagtgat gtgatggctt ctaccaacca
     1621 agtagccgtt gaaatcgccg aaactgaaaa gcaaagaatt gaaagagaga gttcttccca
     1681 attttttcgg cgaatgattg agccgatggt taagggacta tcggtccggg aaaaacaaat
     1741 actagaaaga cgaattcttt gtgaagaagg ttgcgagtct acgcttcagg agttggcaaa
     1801 cgaatgggga ggggtgactc gagaacgggc gcgccaactt gacgttcgag tgcgccgaaa
     1861 attagacacc ttcataaaag atacgccggc agcaaagcga ttgctgcggc agtttccgta
     1921 gccgcccggc tatttgggcg cgaattcttc gaaccgaaac gcggtgtcgc gcttaaatgg
     1981 attcaaaggc cagtaatcgg tcgcgtaggc tttgagttca cccgtacctg gttcgacgta
     2041 aacggctcca ccggctgaga aaaaactttt ctttaatgtc atgtgcttgt tcttgaactt
     2101 tgcgatctgt ggcgcttttc ccgcgtaggg cgagagctcg atcttgtagg tgtcgatcca
     2161 gtctttaccg ccccagggta atccaaacag gggtttactt ttgtaggtgc cgaccaaata
     2221 catttggccg ctgcattcac ttataagttg catgccctga tagttcggga tgtcaaagtt
     2281 accacgctcc gtccaggtgg cttgttcctg aaaacgaaag cccgaggcgt tgagatctga
     2341 atcgttagaa atataaaagg tgagatgaga taactgatga gagcctacgg caaccacgta
     2401 ttttccagcg tgcacgcccc gtttgatttg gctcattgcc acggcttcgg ctcttacgtt
     2461 ggttctaaga attcgccagt cgcgccgctc gacaggactc gtcgggtcag cgtagtcgta
     2521 gaaacgcact tctgagccgg ctttgttatt gttgttgatg tcgattccca ccgctacgat
     2581 tgaaccgaaa gactggatcc cgcccggatg ggagaagtga gagcttaggt gttcgacact
     2641 aacaacgcta tcttttttgg gactagcgca tttgtcgccc ttgcagcgaa cgttggtatt
     2701 ccagtcgcca aactcgggtt gactctt
//
LOCUS       DAOMXO010000303         2720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21337_length_2720_cov_4.188368, whole genome shotgun sequence.
ACCESSION   DAOMXO010000303 DAOMXO010000000
VERSION     DAOMXO010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21337_length_2720_cov_4.188368"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PLH57_07005"
     CDS             complement(<1..909)
                     /locus_tag="PLH57_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02399.1"
                     /translation="MCSYIDQNTMKNKVSPGLLAIALGIVFARTLVELYSFIVTSDIF
                     MDELNSIQAALRMFTHGHYVWDASKLRESFFEPTLSIGLAASWFSGLNWGIFGNLVAT
                     RLGFFVFCFALFCLTMRLVPTQITERKLGPLSMILIWVLFFRFVPYSPAAVSQSLGEF
                     PGAMLILLGVIFLNNGNKKWRVLGPIFLGLSVWHAKLIFLPYALLALFSFQKENPLSS
                     RTAARNLIKECGLFLTPLLAWLLLIGLVNTPTQVGQWLLGKFEFINVVLSGYEYRAVA
                     VSVNIFDRLGSPEFEWFHYGLRWKLEI"
     gene            1017..1637
                     /locus_tag="PLH57_07010"
     CDS             1017..1637
                     /locus_tag="PLH57_07010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK02400.1"
                     /translation="MKIITNVLEQPFAYLLIQNVLGGMRARKLCVQDYCEVSPGSNIL
                     DIGCGPGYVCQYFPNSNYFGFDPSARYIDYAKKKFGTSAKFFAKEFTPSDLETLPRFD
                     LIMLNGVLHHVDDATAHKILSLSGNALNPGGTIMTIDPIFSPQQSFASKFFTKLDRGK
                     NVRYRNQYEQLARDAGLTVEAHERDDLLFIPTTLFVMKSQKTTDSH"
     gene            complement(1621..2421)
                     /locus_tag="PLH57_07015"
     CDS             complement(1621..2421)
                     /locus_tag="PLH57_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HRK02401.1"
                     /translation="MPELAEVATYARDLNAGNRGNKLTKVLFPNQRDWGNVIVPKVVQ
                     NELRGMVKNKIQFSSYGKALVLNLEKTRSHQLEIRLGMTGRFQVDAPAQNWRRHCFLV
                     LEFSNCTIYYLDPRRFGRMRLPAKPAGSAIGGYAIETGFWRSECPATPAGYLRQPRVS
                     WLLGTGAITGVGNYMANEALGRLNLSPFAPCKHQREAESLLVECGRVAEQSFLNGGNS
                     FSPGGAGFANLGGERGNFSEFCLFYKNPKVPRISFRGRPVYSHFSVNL"
     gene            complement(2428..>2720)
                     /locus_tag="PLH57_07020"
     CDS             complement(2428..>2720)
                     /locus_tag="PLH57_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02402.1"
                     /translation="DLKKRPQVRFADPYGNIFDLFQSADCCISFPFGSPGVAASLIGV
                     PCVYYDTTGRLRDSRGATNDVPLIRSLESLRAWMARLKHRSNVKAPKTELEY"
BASE COUNT          823 a          666 c          542 g          689 t
ORIGIN      
        1 tatctcgagt ttccacctaa gcccatagtg aaaccactca aattccgggg aaccgagacg
       61 atcgaaaata tttactgata ctgccacagc tctatattcg tacccagata gtacaacatt
      121 tataaactca aatttgccca agagccactg tccgacctga gtaggagtat ttactaggcc
      181 gatcaacaac aaccaagcaa gtaacggcgt caaaaataag ccacactctt tgataaggtt
      241 tcgagcggca gtccgcgatg aaagtggatt ttctttttga aacgagaaca aagctagtag
      301 agcgtaagga agaaaaatta attttgcatg ccaaacggaa agtccgagaa aaatggggcc
      361 taaaacgcgc cattttttat tcccgttatt caaaaaaatc actccaagga gaatgagcat
      421 ggcccccgga aattcaccaa gactctggga aacggcagca ggactatagg gaacaaatct
      481 aaagaataag acccaaataa gaatcatgct caacggacca agttttcgct ccgtaatttg
      541 cgtgggtacc agcctcatag tgaggcaaaa taaggcaaaa caaaaaacaa aaaaaccaag
      601 ccttgttgca actaaatttc caaaaatgcc ccaatttaat cctgaaaacc aactggctgc
      661 caatcctatg ctcaaggttg gttcaaaaaa gctttcccga agtttagaag cgtcccaaac
      721 ataatgcccg tgcgtaaaca ttcgtagggc tgcctggata ctattgagtt catccataaa
      781 gatgtcactc gtgacgatga aagaataaag ctcaacaaga gtacgcgcaa aaacaatgcc
      841 caatgcaatt gcaagcaggc cgggagatac tttatttttc atagtatttt gatcaatata
      901 agaacacatt atgagtgtca atcgcatcgg tctgatctgg acctacgcac ccacggcgcc
      961 actcttcgaa tttgacaccc atgtctcgat cttatactca taagagtctc ttacctatga
     1021 agatcattac caatgtgctc gagcagccct tcgcttattt attaatacaa aacgttctgg
     1081 gaggaatgcg cgctcgaaaa ctatgtgttc aggattattg tgaagtttca cctgggtcta
     1141 atattttgga tattgggtgt gggcccgggt atgtttgcca atactttcct aactcgaact
     1201 acttcggttt tgatccttcg gcgcgttaca tagattatgc taaaaagaaa tttggcactt
     1261 ccgccaaatt ctttgcaaaa gaattcactc ctagcgacct agaaacattg ccacgctttg
     1321 atctgataat gctcaacggc gtgcttcatc atgtagatga cgcaactgct cacaaaatac
     1381 tctcgctttc gggaaatgct cttaatcctg ggggaacgat tatgactatc gatccaatct
     1441 tttctcccca gcaatcgttt gcctcaaagt tttttacaaa attagataga ggcaaaaatg
     1501 tgaggtaccg taatcaatac gagcaattag ctagagatgc aggcctaacc gttgaagcac
     1561 atgagcgaga tgacttatta tttattccga ctacactttt cgttatgaag tctcaaaaaa
     1621 ctacagattc acactaaaat gggaatagac tggacggcct cgaaaactaa ttcttggcac
     1681 tttcggattc ttataaaata aacaaaattc ggaaaaatta ccgcgctcgc cccccaagtt
     1741 cgcgaaaccg gcccctcctg gactaaacga attgcctcca ttcaaaaagc tctgttcggc
     1801 gactcggcca cactctacaa gcagactctc agcctctctt tggtgtttac atggtgcaaa
     1861 cggcgaaaga ttcaggcgcc ctaaagcctc attggccata taattgccga ctccagtaat
     1921 tgcaccggta ccaagcaacc aagaaactct gggctgccgc agataacccg caggtgttgc
     1981 ggggcactcg gaccgccaaa accctgtttc gatggcgtaa ccaccgatcg cacttcccgc
     2041 tggtttagct ggcaacctca ttcggccgaa tctcctcgga tcgaggtaat aaatggtgca
     2101 attcgaaaac tccaaaacta aaaaacaatg tcgccgccaa ttttgggccg gagcgtccac
     2161 ctgaaatcgc ccggtcattc ccagacgaat ctctagttgg tgtgatcttg ttttttctaa
     2221 attcaaaaca agcgcttttc cataggaaga aaattgtatc ttatttttaa ccattccccg
     2281 cagctcattc tgtaccactt taggaacaat gacgtttccc cagtcgcgct gatttggaaa
     2341 caacactttg gttaacttat ttccgcggtt cccggcattt aaatcccgcg cataggtggc
     2401 aacttctgcg agttcaggca ttaatgatca atactccaat tcagtcttcg gtgctttcac
     2461 gttggagcga tgctttagtc tagccatcca cgctctcaaa ctctctaagg aacgaatcag
     2521 cggcacatca tttgtggcgc ctctcgaatc cctaagacga cccgtggtat cgtagtaaac
     2581 acagggtact ccgatcaggc tcgcggccac cccgggcgaa ccaaaaggaa aactgataca
     2641 acaatcagcc gactgaaaaa gatcaaaaat atttccgtag ggatctgcaa acctcacttg
     2701 agggcgtttt tttaaatctt
//
LOCUS       DAOMXO010000304         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21366_length_2717_cov_5.308039, whole genome shotgun sequence.
ACCESSION   DAOMXO010000304 DAOMXO010000000
VERSION     DAOMXO010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21366_length_2717_cov_5.308039"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLH57_07025"
     CDS             <1..373
                     /locus_tag="PLH57_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02403.1"
                     /translation="LDLGAMERELCGSETRRMTHLVASVCFTGNAVALATRNLEMLAG
                     PSRVRIGYDEIGEPVEILMDVTALFRDPSRDLKSLLPNKFNRKGHAISYPDPTMGGLF
                     PDGDLIERLKVIDAPGVSHID"
     gene            402..863
                     /locus_tag="PLH57_07030"
     CDS             402..863
                     /locus_tag="PLH57_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02404.1"
                     /translation="MGQRNVRLLRCFTIALMFGLTCPSAFATKERFETLTFDTASSEC
                     RVQIAPYAQTISWSDGTALEVVAASGCASRLGRHYELQGDVRLTLLDAKRLPLLQVTA
                     NNADLDLANKRVVFPAEAKVNYRLKQMTLPGLLLNWNSRETKSFSGFYARF"
     gene            complement(867..2375)
                     /locus_tag="PLH57_07035"
     CDS             complement(867..2375)
                     /locus_tag="PLH57_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRK02405.1"
                     /translation="MRNEFSRSGIARSIFTFFILFTAKPSSAELLQIIHTNDLHSHFE
                     SYPALKATIDRIKNDAGSQGIETLVLDAGDFSEGTQFYLADRGEQSWRAMSELGYDAV
                     TIGNHDYLLGLKDLNRVIKNVKPSHYLLGANFRVGKSYPDLKKALTSRARFERGGKHI
                     SVIGLTTDEFMYTWRAGKKVISSPIQAAQVLVPKYRRDSQFVIALSHLGIEEDLKLVR
                     NTRGIDLVIGGHSHTKLSRPYHQTDLDHKLVPIVQAGDHGKYVGDLLVDLVPGKPLRI
                     VRYELHPVSLHGPRDSGLERFLADAREKLKAMYPPNWLSQPIGFSEVPLEVPGRGQRT
                     AWSEILGRGMMRVTKADLAFNASDLYGTSVPAGIVTREKLIQYYPRVFEFYNHTTGWN
                     VWTLEVEGWILKPFLENVIRQGFRVTTTNVTFDVSGAKGKESISNFRVGNSEVAWYKT
                     YKVAVPEGIGRVIAGDWYNSRLLQPVQSSKVPIWAAMEIQLSKQGGVVRAYD"
     gene            complement(2536..>2717)
                     /locus_tag="PLH57_07040"
     CDS             complement(2536..>2717)
                     /locus_tag="PLH57_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02406.1"
                     /translation="GFKKKYTAKRVTDPKKPPIPTKSIWFNVIDFVYPMPVPIRHRTK
                     SKCTRSQSKCALRRQ"
BASE COUNT          637 a          655 c          687 g          738 t
ORIGIN      
        1 tctagatctc ggtgcgatgg aacgagagtt atgcggtagc gagactcgtc gaatgaccca
       61 tctcgttgct tcggtctgtt tcacaggtaa cgccgtggca ttggctaccc gtaacttaga
      121 aatgttggct ggcccctctc gcgtgcggat cgggtacgac gaaattgggg agccagttga
      181 aattctcatg gacgtgaccg cgttgtttcg cgatccttcg agagatctca agagtctttt
      241 gccgaacaaa ttcaatcgca aagggcatgc gatttcttac cccgatccta caatgggcgg
      301 gttgttcccc gatggcgatt tgatcgaacg acttaaagtt atcgatgctc caggcgtcag
      361 ccatatcgac taagcgaagt aaaatgcgga gtgaactact catgggtcag cgtaacgttc
      421 gactactgcg ttgtttcacg atagcgctga tgttcggact gacttgtccg tcggcattcg
      481 cgactaaaga gcgtttcgaa acactcacgt ttgataccgc gagttccgaa tgtagggttc
      541 aaatcgctcc ctacgcccag accataagtt ggagcgatgg gactgcgctc gaggtggtag
      601 ctgcgagtgg ttgcgcatca cgcttggggc gccattatga gcttcaaggg gacgtgcgcc
      661 ttacgctgct cgatgcgaaa cggttaccgt tgctacaagt gacggcgaac aacgccgatc
      721 tcgaccttgc caacaaacgg gtggtgttcc ccgccgaagc caaagtgaat taccgcttaa
      781 agcaaatgac gcttccgggg ttgttactga actggaactc gcgtgaaaca aagtcgttta
      841 gtggtttcta cgctcgcttt tagaacctaa tcataggccc gcacgacgcc gccttgtttg
      901 cttagctgaa tttccattgc tgcccatatt ggtaccttgg aagactgtac tggttgtaaa
      961 agtcgagagt tgtaccaatc accggcgatg actcggccta ttccttcggg caccgctact
     1021 ttgtaggttt tgtaccaagc aacctccgaa ttaccgaccc taaagttcga aatgctttct
     1081 ttaccttttg cgcccgaaac gtcgaacgtc acgttggtgg tcgtcactcg aaaaccttgg
     1141 cgtataacat tttcgagaaa gggttttaga atccacccct cgacttcgag cgtccataca
     1201 ttccaacccg tcgtatgatt atagaactca aacactctag ggtaatactg aattagtttt
     1261 tcgcgagtaa cgattcctgc gggaactgaa gtaccgtata gatcgctcgc attgaacgcg
     1321 agatcggctt ttgttacccg catcattccc cgtccaagaa tttcactcca tgcggtgcgt
     1381 tgtccccgcc ccgggacttc gagaggaact tcgctgaagc cgatgggttg ggacaaccag
     1441 ttgggtggat acatcgcctt aagtttttca cgggcgtcgg ctaagaatcg ttcaagtccc
     1501 gagtcccgag ggccatgtaa gctcaccggg tgtagttcgt aacgaacaat tcgcagtggt
     1561 ttgccaggga ctaagtctac cagtaagtct ccgacatact taccgtggtc gcccgcctga
     1621 acgatcggaa ccaatttgtg atcgagatcc gtttggtgat agggccgtga aagtttagtg
     1681 tgagagtggc caccgataac cagatcgatt cctcgagtgt ttcgaactag tttgaggtct
     1741 tcttcgattc ctaagtgcga aagagcaata acgaattggc tgtctctgcg gtatttcggc
     1801 accaacacct gagcggcttg gattggagaa gaaattactt tttttccagc gcgccaagtg
     1861 tacatgaact catcggtggt tagcccaatg acagatatgt gtttgccccc acgctcgaaa
     1921 cgagcgcggc tggttagtgc tttttttaag tcgggataac tcttacccac gcggaaattg
     1981 gcacccaata gatagtggct gggttttaca ttcttaatga cccggttcaa gtctttcagc
     2041 cctagtagat agtcatggtt gccaatcgtg acggcatcgt aaccaagctc actcatggct
     2101 ctccaagact gttcgcctcg gtccgctaga tagaattgag tcccttcgct aaagtcccca
     2161 gcgtcgagca ctaaggtttc aatgccctgg gaaccagcgt cgttttttat tcgatcgatg
     2221 gttgctttca gggccgggta actttcaaaa tgcgaatgaa gatcgttggt gtgaattatt
     2281 tgcaagagct cagcgcttga aggcttagcc gtgaaaagta taaaaaatgt aaagatagac
     2341 cgcgcaatac cggaacgtga gaattcgttt ctcatttttc gtgcactcca tgcatgaaat
     2401 ttcgtttgtc acgatgcgct cgctacgatg tgttggtttg attttagaaa ttgaaattac
     2461 acttcggccc accccctcca acccatagtt tagcagacgc cgtgccaagt gcacaaagtt
     2521 gttaagtgtt tgttcttatt gtcgacgcaa tgcgcatttt gattgcgacc tggtgcattt
     2581 cgactttgtg cggtgacgaa ttgggaccgg catgggataa acaaaatcga tgacattgaa
     2641 ccaaatactt ttagtgggga ttggtggttt tttaggatca gtcactcgct ttgcagtgta
     2701 ttttttttta aatccct
//
LOCUS       DAOMXO010000305         2715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21394_length_2715_cov_4.771429, whole genome shotgun sequence.
ACCESSION   DAOMXO010000305 DAOMXO010000000
VERSION     DAOMXO010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21394_length_2715_cov_4.771429"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLH57_07045"
     CDS             complement(<1..219)
                     /locus_tag="PLH57_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02407.1"
                     /translation="MNLLAATSFLSLPIVAVNFWIYSKGTNKIFAVSQLQKVLIPTLI
                     VAILAFVLIRKASWLGFLLTAGFTTFVVS"
     gene            303..503
                     /locus_tag="PLH57_07050"
     CDS             303..503
                     /locus_tag="PLH57_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRK02408.1"
                     /translation="MPFLIDKNCTRCGACLPECPTGAIAEAEPIFLIDADVCSDCMAC
                     VPVCPVHAIQRIPEAPKTEPVK"
     gene            500..1681
                     /locus_tag="PLH57_07055"
     CDS             500..1681
                     /locus_tag="PLH57_07055"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRK02409.1"
                     /translation="MNHSVFSLGVMTGTSCDGADGAVLKIDPETESESLYGTYSRKFP
                     ANLRLQLRKAQSGKLNIVDAGRIELLYSDWLAQFCSQVISKFKLNTARTLCSIHGQTV
                     WHEPPRFSIQLGEPARIAIKTGLTTIARFRQPDLALGGQGAPLVPYYHWLRAKQMRQT
                     QKNPFSIHNIGGISNLTVVTHKLDEIFAFDTGPGNALIDLAVEKVTKGRHHFDRSGRM
                     AESSLSQIDWGKIEKLSKHPYFCETPPKSTGRELFNEAFLRKIPGQGARLVANATAFT
                     AHTVAKAYRDFVISRFPKFDCIYIAGGGAANNVLVLLIQREIDRLLPKKNIRVAMLPK
                     TLGPLHYLEAMAFARLGYEALKGREISLGAVTGARSNGIGAAIIPGKNFQSLLRRIAL
                     I"
     gene            complement(1652..2653)
                     /locus_tag="PLH57_07060"
     CDS             complement(1652..2653)
                     /locus_tag="PLH57_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRK02410.1"
                     /translation="MKALVLCAGFGTRLRPLTDNFSKPALPIVGVPTVWYAIWSVLRE
                     LQPDRWMVNVGHCPETVHNALEIADLRNFARIAIDYSDESQQILGSSGALRKIKNWVG
                     NDRLLVVNGDSVCFAPWRKMLEFHVNRKSPLTIHVRAFNGSEPYTNVAVDSKGLVVGF
                     GEKSNQGTMFSGAYIIEPELLERLPDGVSELRPSLLEPLVQEARLYAYREDIEWFDTG
                     TLATFFSAQFDVPSKLRAARPLIESTMYEFAPGSWRPKTWGGFRNKKFDVRNPVIVVG
                     APEYWENLAIENFGPRFCGISDYLGFGGDKARFEKSLLENGTSDTLVFLNQSYSAKK"
BASE COUNT          755 a          682 c          611 g          667 t
ORIGIN      
        1 actaactaca aaagtagtaa aacccgccgt caataagaag cccaaccaac tggccttccg
       61 aattaaaacg aaggctaaaa tagcaactat caatgttgga attagtactt tttgaagctg
      121 ggaaacggca aagatcttgt tggtgccctt tgaataaatc cagaaattga ccgctacaat
      181 aggtagcgat aaaaaagacg ttgcggccag aagattcaat agcggcggcc ttcttaagaa
      241 gcgcatgata ctagtgtgat tcaccttgga tatttgtcaa actatgttac cgtttatgca
      301 atatgccgtt tttaatcgat aaaaactgca cccgatgcgg agcctgttta ccagagtgtc
      361 caaccggtgc aattgccgaa gccgaaccaa tcttcctaat tgatgcagat gtttgctcgg
      421 actgcatggc ctgtgtacca gtttgccccg tacatgccat tcaacgtatt ccagaggcgc
      481 ccaaaacgga acccgtgaaa tgaatcatag cgttttctcc ttaggggtca tgactggaac
      541 cagttgcgat ggagcagacg gcgccgtgtt aaaaattgat cctgaaacgg agagtgagtc
      601 tctttacggg acttattccc ggaagtttcc cgccaatctt agactccaac tgcgcaaagc
      661 ccaatcggga aaactaaata tcgtcgacgc ggggcggatc gaactgctgt actctgattg
      721 gcttgctcaa ttttgcagtc aagtcatttc caagtttaaa ttaaacaccg cgcgcacatt
      781 atgttcgata catggccaga ccgtatggca cgaaccgcca cgcttttcga tccaacttgg
      841 ggaacctgcc cggatcgcga ttaaaaccgg gctaaccacc attgcgcgct ttcggcagcc
      901 agaccttgcc ttgggtggcc aaggtgcgcc attggttccg tactatcatt ggctacgcgc
      961 caaacagatg cgacagaccc aaaaaaatcc cttttcaatt cataatattg ggggaatttc
     1021 gaacctcacc gtagtcactc acaagttaga tgaaatattt gcgtttgaca ctgggccagg
     1081 aaatgccctc attgatttag cggtggaaaa ggttaccaaa ggtcgtcatc actttgatcg
     1141 cagcggacgt atggccgaaa gcagtctctc gcaaatcgac tggggtaaaa ttgaaaaact
     1201 atcgaaacat ccttattttt gcgaaacgcc cccgaaaagt acgggaagag aactttttaa
     1261 cgaagctttt ctaagaaaaa taccaggcca aggggcgcga cttgtagcca atgccactgc
     1321 ctttaccgcg cacaccgttg ctaaagccta tcgcgatttc gtaattagcc gttttccgaa
     1381 atttgactgc atctacatcg ccggaggggg cgccgcaaat aacgttcttg tactgttaat
     1441 tcagcgagag attgatcgtc ttttgcccaa aaaaaatatt agagttgcga tgctgcccaa
     1501 gacattggga ccgttacatt atttagaggc catggccttt gcccgacttg gatatgaagc
     1561 actcaaggga cgtgagattt ctcttggcgc ggtcacaggg gctcgatcaa atggaattgg
     1621 cgccgccatt attcccggaa aaaactttca gtcacttctt cgcagaatag ctttgattta
     1681 agaatactaa cgtatcggaa gtaccatttt ccaacaggga tttttcaaac ctcgctttat
     1741 cgccaccaaa tccaaggtaa tcgctaattc cgcaaaagcg cggcccgaag ttttctatgg
     1801 cgagattctc ccaatactcc ggtgcaccaa caacgatcac cggattgcgg acgtcaaatt
     1861 ttttgttacg aaatccaccc caggttttgg gccgccaaga accgggagcg aattcataca
     1921 ttgtggactc gataagcggc ctcgctgctc gtagtttcga cggtacgtcg aactgtgcgg
     1981 aaaaaaaagt ggccaacgtt cccgtgtcga accattcgat atcttcgcgg taggcataga
     2041 gccgcgcttc ttgaaccaac ggctccaaga gcgagggtcg caattcgctt actccgtcgg
     2101 gcaatctttc taataactcc ggctcgatga tgtaagctcc cgaaaacatc gttccctgat
     2161 tcgatttttc gccaaaacca acaaccaagc ctttcgaatc gactgcaaca ttggtgtacg
     2221 gctcagaacc attaaacgcc cttacatgaa tagtgagcgg tgactttcga tttacgtgga
     2281 attcgagcat tttgcgccag ggagcgaagc atacgctatc cccatttacg accaacagcc
     2341 gatcattccc cacccagttt ttaatttttc gaagagcccc agaagagcct aatatctgct
     2401 gcgattcgtc actataatcg atagcgattc ttgcaaaatt tctcaaatca gcaatttcta
     2461 atgcgttatg aacggtctcg gggcagtgcc ccacattgac catccagcgg tcaggctgaa
     2521 gctctcgcaa caccgaccaa atcgcatacc aaacggtagg gactcccaca atcggaaggg
     2581 ctggcttcga aaagttgtca gtcaggggtc gaagtcgggt accgaagccc gcacacaaaa
     2641 caagtgcttt catttttaac ccttaaaaat agtaatatcg aaaaagcaga catctaagct
     2701 cgtcgagctc tggga
//
LOCUS       DAOMXO010000306         2710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21446_length_2710_cov_6.890772, whole genome shotgun sequence.
ACCESSION   DAOMXO010000306 DAOMXO010000000
VERSION     DAOMXO010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21446_length_2710_cov_6.890772"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1652
                     /locus_tag="PLH57_07065"
     CDS             87..1652
                     /locus_tag="PLH57_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02411.1"
                     /translation="MKTNVKNSWPLIIGTLSLAGLSLGGNCPGTGLSAKFSSIGDSVS
                     ATAAKSWRTAAIMDPNSIDGNTFDQNIILPLGTIGPHSMSVFAESASSFFVMFSRETA
                     TGDVSVYGTQPITFATGSWPTTMGGFTLLDTETAADEARNPVTAVDTLGNSVTVYLQD
                     PGGGAETRRVNAVRRLASSAAWISPVDLRASAEVAAAVDVGNESLAIAVDAYNAFVPV
                     WCSSNNILYFNHYRSSVGWRFNTSSTADATWDGASDCDNSLGVDIAFDGYGNGYGAFV
                     TTGDEVKVRRWQGDTAANWLAAGYKDITNGTTACYPRLFMTPDGEGYLFFYTNCAGAA
                     GDLYYSYAAATSTSSPPVSSDFGAAAAFDTGIGTGNAYIQDNGDPIPPILAVNGNYAV
                     IGHIKQDDGLMYRLWVSRYDGNSWSTHTAIDQRFGSVTNAALAINSNGEIAAMYSAVY
                     NGSEVIYVTNYTNGVWKTPTQVSLPGDLVPGNGATARYHPSIAIDASGNAIGTFTMTD
                     TGGNRRAVAVMYR"
     gene            complement(1653..>2710)
                     /locus_tag="PLH57_07070"
     CDS             complement(1653..>2710)
                     /locus_tag="PLH57_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HRK02412.1"
                     /translation="GFVASVLPVWLLLCPRDYLSSYMKLGTIAFLVIAVLIVQPDLKM
                     PPITQWADGGGPIIPGKLFPFVFITIACGAISGFHSLVASGTTPKMIANEMHIRPIGY
                     GAMLLEGLVGVVALIAAAALNPEDYFAINVSASKFAQLGMATVDLPHLEAQVGENVAG
                     RPGGAVSLAVGMAKIFSSLPFMERLLGYWYHFAIMFEALFILTTIDTGTRVARFLVQE
                     SGGKLWPRFGKNDWLPGALISTALVVGGWSYFIWTGSISTIWPMFGISNQLLASIALC
                     VGTTFIVNSKRRRRYAWVTLAPLAFVGTITVTAGYQTIRYNFIPNGAYLNAILTAIMM
                     VSLGIVLACSIPKWLKK"
BASE COUNT          686 a          674 c          713 g          637 t
ORIGIN      
        1 ttttagtgtc gtttaccgcg aactccggta ccggcaccac gcgcagacaa tacgtaaaac
       61 gctatttacc gataggattg tgacgaatga aaacgaacgt aaaaaatagc tggcctctaa
      121 taattggcac actttctctc gcgggtttga gtctcggagg aaactgtccg ggaacagggc
      181 tttctgcaaa atttagttcg atcggcgatt cagttagcgc gacggcagca aaatcttggc
      241 gtaccgcagc catcatggac cccaactcga tcgatggaaa tacttttgat caaaatataa
      301 ttttgccact cggaaccatt gggccccatt ctatgtcggt gttcgcggaa tcggcgagtt
      361 cattttttgt catgttctct cgtgaaaccg ccactggtga tgtttcggtt tacggcacgc
      421 aaccaattac ctttgccaca gggtcgtggc ccacgaccat gggagggttt actttattag
      481 ataccgaaac tgcagcggac gaagcgcgca atccggttac ggcagtcgac acactcggaa
      541 attcagtgac tgtttatttg caggaccctg gcggtggagc tgaaactcgc cgggtaaatg
      601 cggtacgccg gttggcaagt agtgccgcgt ggatttcgcc agtcgatcta cgcgcgtctg
      661 ccgaagtcgc tgcagccgtt gacgttggaa acgaatcgct tgcgattgcg gtagatgcct
      721 acaatgcgtt tgttccggtg tggtgtagtt cgaataacat cttatatttt aatcattacc
      781 gatcaagcgt tggttggcgg ttcaacacca gttccacggc agatgcgacc tgggatggag
      841 ctagtgattg cgacaattct cttggcgtgg acatcgcatt cgatggatac gggaacggtt
      901 acggcgcttt cgtgaccacg ggtgatgaag ttaaagtgcg ccgttggcaa ggagatactg
      961 ccgccaattg gcttgctgct gggtacaaag acattaccaa cggaaccacg gcctgttacc
     1021 cacgcctttt tatgactcca gatggagaag gttatttatt tttttacacc aactgtgccg
     1081 gtgccgcagg ggatctctac tattcctacg cagccgcgac ttctacgagt agcccgccgg
     1141 tgagttcaga cttcggagca gctgcggcgt ttgatacggg tattggaact ggaaacgctt
     1201 atattcaaga caacggagat cctattcccc caattctggc ggtgaatggc aactacgcag
     1261 tgatcgggca tataaaacaa gacgacggat taatgtaccg actttgggtt tcccgttacg
     1321 acgggaactc ttggtcgaca catacagcga ttgatcaacg ttttggttca gtgaccaatg
     1381 cggctttggc aattaattcc aacggtgaaa tcgccgcgat gtattcggcc gtctacaatg
     1441 gttctgaagt aatctacgtc acgaactaca ccaatggagt ctggaaaact ccaactcaag
     1501 tttcattgcc gggggatctc gtgcctggca atggtgctac cgctcgttac cacccgtcga
     1561 tcgcaatcga cgctagcggt aacgcaatcg gtaccttcac catgacagac accggcggga
     1621 accgccgtgc ggtcgcagta atgtatcgat gattattttt ttagccactt cggaatggag
     1681 caggcaagca cgattccaag gctcaccatc atgatggcag ttagaattgc atttagataa
     1741 gcgccattag gaataaaatt gtaacgaatg gtttggtagc cggctgtaac cgtaatggtt
     1801 ccgacaaacg caaggggagc caacgttacc caagcataac gacgtcgtcg tttggaattc
     1861 acgataaatg tcgtgcccac gcagagtgcg atgctggcta gtagctgatt actgatgcca
     1921 aacatgggcc aaatcgtaga tatgctgcca gtccaaatga agtaactcca gccgcccacc
     1981 acgagtgctg tcgaaatcaa ggcaccaggg agccagtcat tttttccgaa tcggggccaa
     2041 agtttaccgc cagattcctg cacaagaaag cgcgctacgc gagtgcctgt gtcgatcgtg
     2101 gtcaaaatga aaagcgcttc gaacatgatt gcgaagtggt accagtatcc gagtagtcgc
     2161 tccatgaacg gaaggcttga aaaaattttt gccatgccga ctgccagcga cactgcgcct
     2221 cccgggcgcc cggctacatt ttcgccgact tgggcttcga ggtgcggcaa atctaccgtt
     2281 gccatgccga gctgcgcgaa tttagaggcc gacacattga tagcaaaata gtcttcgggg
     2341 tttaatgcgg cagccgctat tagagctacg acaccgacca gaccctctaa caacatagcg
     2401 ccgtagccga tcggtcgaat atgcatctcg tttgcgatca tctttggagt ggtgccgctt
     2461 gcgacaagag aatgaaaccc agaaatggca ccacatgcaa ttgtaatgaa aacgaacgga
     2521 aacagcttgc cagggataat gggcccgcct ccatcggccc actgggtgat cgggggcatt
     2581 ttgagatcag gctgaacgat caacacagcg attaccaaaa aagcgatagt cccgagtttc
     2641 atgtacgagc ttaaatagtc gcgcgggcag agaagcaacc acacgggtaa aacgctcgcg
     2701 acgaaaccgt
//
LOCUS       DAOMXO010000307         2703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21513_length_2703_cov_4.887085, whole genome shotgun sequence.
ACCESSION   DAOMXO010000307 DAOMXO010000000
VERSION     DAOMXO010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21513_length_2703_cov_4.887085"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLH57_07075"
     CDS             complement(<1..552)
                     /locus_tag="PLH57_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02413.1"
                     /translation="MQVNVDINQKKIKPAAKLLSRVPEPVIQSCRKLQGAGHIAWLAG
                     GCVRDLILGREPRDWDVVTDAPLDTIKTIFPKHLDVGVVFGIVKLPPIQNVQIDIAIF
                     RKEEGYTDRRHPDVVTTGSPAEDAARRDFTINALYLDLATSEMIDFFTGAKDLDAKIL
                     RSVGDPNKRFSEDALRVLRAVRLS"
     gene            complement(564..833)
                     /locus_tag="PLH57_07080"
     CDS             complement(564..833)
                     /locus_tag="PLH57_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02414.1"
                     /translation="MKWGRIFCLLLLLISTPSLAEQDVDTAAGTAPATTSQTDMSEAD
                     LETTESESESASKNHKTPSRREKRVQSKPIRTQRSAPEKLEVDPD"
     gene            896..2152
                     /locus_tag="PLH57_07085"
     CDS             896..2152
                     /locus_tag="PLH57_07085"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRK02415.1"
                     /translation="MKSTIEVQGTLRLSGQTSRRAPRYVRGAPWVYSNEIEPPKSKFA
                     PGSWAVLESAEGRILGTGFYNPHSLIAFRFFQRGAEFQGAQFESEVRARLSAALVLRQ
                     RALADYINSGELGRNSFRLVFGESDGLPGLIVDLFESDNGKPVGVVQCHAAGADAFIS
                     VLWSWMSSELGAQALVVRNDVEVREREGAAIFTETLGVLPSRTFAREGGVEFEASLLR
                     GQKTGYFYDLRHHRRHFAAALRGHRGVVLDAFSYIGSWGLQVLKQNPNLELLAADTSR
                     AALDILEANAAANGLKNRVRTVECDLMKDDGRVFEKRSFAGVVCDPPALTQSAKQLEA
                     GRRAHVDVFSRALSAAAAHGPVALASCSFHLGIEGFLECVSDASFKAQRSYLMTELGT
                     QDVDHPVLAALPESRYLKCALGVVTT"
     gene            complement(2142..>2703)
                     /locus_tag="PLH57_07090"
     CDS             complement(2142..>2703)
                     /locus_tag="PLH57_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02416.1"
                     /translation="VATFCFVIAVQAFASEELRTKSLSCRLWTNSAEFDDTDIPDFAV
                     PLVDVSNETPGRARLQVNSIDKKKMMDIELIFHTHTDSKLIGKVKSTDQTLGEDGRKI
                     ELRTVIRTASVHVEPGPKPGEPSFKIDVKGHSKSDDNQCFPKLKFVCLASEEKVENPT
                     PTPTATPTATPTPSASPEVITPAFRS"
BASE COUNT          588 a          627 c          759 g          729 t
ORIGIN      
        1 agacaaacgg acggcccgca atacgcgcag cgcgtcttcg gaaaaacgtt tattcggatc
       61 gcctactgac cgcaatattt tggcatcaag atctttggcg cccgtaaaaa agtcgatcat
      121 ttcgctagta gcgagatcaa gatagagcgc gttaattgtg aaatcacggc gagcagcgtc
      181 ttcggcggga ctgcccgttg ttacaacgtc cgggtgtcgg cggtctgtat agccttcttc
      241 tttgcgaaaa atggcaatat cgatttgcac gttttgaatt ggaggaagtt ttacaattcc
      301 gaataccacg ccaacgtcga ggtgtttggg aaaaattgtc ttaatggtat cgagtggtgc
      361 atcggttaca acatcccagt ctcgcggttc acggcctaaa atcagatcgc gcacacaacc
      421 tcccgcgagc catgcaatgt gaccggcgcc ttggagtttg cggcaagact gtatgacggg
      481 ctcgggaact cgactcagta gttttgcggc aggtttgatt tttttttggt tgatatccac
      541 gttgacctgc attggtgaaa agcctaatcc ggatcgactt ctagtttttc gggagcactt
      601 cgttgcgtcc gaattggttt agattggact cttttttcgc gtctgctggg ggttttgtga
      661 ttcttcgaag cgctttcgct ttcactttca gtggtctcaa ggtctgcttc cgacatgtca
      721 gtttgcgagg ttgtggctgg ggcagtcccg gcggcggtgt ctacgtcttg ttcggcaagg
      781 ctcggcgtgg aaattaataa cagcagcaaa caaaagattc ggccccattt catcgccatc
      841 gtgttaaaaa actatcatcg actttatagg cctgtaaagg gaggtatcca acgtcatgaa
      901 atcaacgatc gaagtacaag gaacactgcg tctatctggc caaacctccc gacgggcacc
      961 gaggtatgtg cggggcgcac cctgggtata ttctaatgaa atcgaaccgc ctaagtctaa
     1021 gtttgcgccg ggtagctggg cggtattgga gtcggctgaa ggccgaattc ttggcaccgg
     1081 gttttataat ccgcactcgt taattgcgtt tcgttttttt cagcgcggcg cggagtttca
     1141 gggggcgcag ttcgaatccg aagtgcgggc ccgcctcagc gcagcgcttg tgttacgcca
     1201 gcgtgctttg gcagattata ttaattcagg ggaactcggg cgtaattcat ttcgtttggt
     1261 ttttggcgaa agcgacgggt tgcccgggtt gatcgtagat cttttcgaaa gcgacaatgg
     1321 aaaaccggta ggggtggtgc agtgtcatgc ggccggtgcc gacgcgttta tatcggtact
     1381 ctggtcttgg atgagttcgg aactcggagc acaggccctg gtggtgcgca atgacgttga
     1441 agtccgggaa cgagaaggcg ctgctatttt taccgaaacc ttgggagtcc tacctagtcg
     1501 cacttttgct cgtgaagggg gagtggagtt tgaggcttcc ttacttagag gacagaaaac
     1561 cggctacttt tacgacctac gccatcaccg acgtcatttt gcagctgcgc ttcgtgggca
     1621 ccggggggtt gttttagatg ccttctccta cattggaagt tggggccttc aagttttaaa
     1681 acagaatcca aacctggaac tgttagctgc cgatacgtcg cgggcggcgc tcgacatttt
     1741 agaggccaat gccgcagcta acgggctaaa gaaccgggtg cgcacggtcg aatgcgattt
     1801 gatgaaagat gatggccgag tttttgaaaa acgatcattt gctggcgtgg tctgtgatcc
     1861 accggcgctg actcaatcag ctaaacagct agaggccggt cgaagagccc atgtcgatgt
     1921 atttagtcgc gctttgtcgg cagccgccgc ccatggcccg gtggccctag cttcgtgcag
     1981 ttttcatttg ggaattgaag gatttttgga atgtgtctcc gacgcttctt ttaaagccca
     2041 gcgctcctat ttgatgacgg agcttggcac ccaagacgta gaccaccctg ttttggctgc
     2101 gctgccagag agtcgttacc tgaaatgcgc tctcggagtc gttacgacct gaatgcgggg
     2161 gtgatgactt ctggtgaggc cgaaggcgtg ggagtcgcag ttggagttgc ggttggagtt
     2221 ggggttgggt tttcaacttt ctcttccgac gccaggcaca cgaatttcag tttgggaaaa
     2281 cattggttgt cgtcagattt cgaatggcct ttgacatcga ttttgaacga gggctctccg
     2341 ggcttaggac cgggttctac atgaacactg gcagttctta taacggttcg aagctcaatt
     2401 tttcggccgt cttcacccaa agtttgatcg gtagatttta ctttgcctat taattttgaa
     2461 tcagtgtggg tgtgaaaaat tagttcaatg tccatcatct tctttttatc gatgctgttg
     2521 acttgaagtc gcgcccgccc aggagtttcg ttggaaacgt caactagcgg tacggcgaaa
     2581 tccggaatat cggtatcgtc aaattcggca ctgttggtcc acaagcgaca cgacagagat
     2641 ttcgtccgta gttcctcgct cgcaaaagct tgaaccgcga ttacaaaaca aaaagtcgcg
     2701 acc
//
LOCUS       DAOMXO010000308         2703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21516_length_2703_cov_4.269637, whole genome shotgun sequence.
ACCESSION   DAOMXO010000308 DAOMXO010000000
VERSION     DAOMXO010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21516_length_2703_cov_4.269637"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLH57_07095"
     CDS             <1..480
                     /locus_tag="PLH57_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02417.1"
                     /translation="TIVIVALVVAFLFKNKKAEPAKDFSSVVNKMRELVIGLDIEIGE
                     LQRRAKHASEDLSERTKNLASRMTEQWAQVRVIAINGAIEATKDPELQKRFQRYFDEI
                     GKTVTESRELCLELEKLGRPAPELMEQIKDLDELLARVADDAIVFSPRVSGQKRRPE"
     gene            complement(389..1411)
                     /locus_tag="PLH57_07100"
     CDS             complement(389..1411)
                     /locus_tag="PLH57_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRK02418.1"
                     /translation="MKMQKIKEIWQVNNDSAQKNKTEQSQRSESRSLLPAIELIGASD
                     DRGVRNVGGRLGAAHGPTTIRNSLEAMAIGMSLDTLNLLKGYDVPAGKSIEEGHMALR
                     TAVTQSLKSDRTPIVLGGGHDYGFPHLAGVVDFKKSDVALINIDAHLDVRPPNEHGIT
                     SGSPFFLAIESGVLNPKLFFELGIQDHCNTKEAQAYLTKRGATVHTLRSMRNSKSGVI
                     STFREVLKSLAHKNIATVVSFDLDAVSMAWAPGVSAPQIDGLTAGEMIEMARICGEYS
                     IVRSIGFFELAPPLDECGKTARLAATAIHQYSSVLTRGVASGRKPGAKTRLRRLLRVP
                     KARRGL"
     gene            1426..>2703
                     /locus_tag="PLH57_07105"
     CDS             1426..>2703
                     /locus_tag="PLH57_07105"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HRK02419.1"
                     /translation="MELDPGRNTEEKKWFLYVVDHHEGPFSLSEIQSLVKKGDAKSSS
                     YVWREGFEDWMMMSEVSEFGVAGPSGEGHGPAAFILGKKESPVLFSPRSSDASVGGPL
                     PIAHQGNVPDISGISETAVVWCLNSNRTFTGPHSLRTITRLINEGKATSKDTIWKEGW
                     TGFVPISSFSEFMVRIHDGASQKNKVSSAQALGMMRSGMGIKRRFSFGGILKWLVLAF
                     LVFGGFYQVTAMGLLNPVFEKIGILETVRRANLKPVPVPKIVIPVEYISDAGNFLRRQ
                     VEPLLAKYSDHIPGPILEYLSVVELPEGLNEKDEARLRDAATANPDAGRRAAAALIGQ
                     EVMNPRFVLASNIDGSSFRVVLRGKEGTLLNASEYVSEQVVTLRNRVAISQPFYLEDT
                     KSLPQGEYTFTVLNGEESVLIENAFLGGENDSAY"
BASE COUNT          717 a          592 c          690 g          704 t
ORIGIN      
        1 actatcgtga tcgtcgcttt ggtggtggct ttcttgttta aaaacaaaaa agcggaacct
       61 gccaaagatt tttcgagcgt ggttaacaag atgcgagaac tagtaattgg tctggacatc
      121 gaaatcggcg aactccagcg ccgagccaag catgcgtccg aagatttatc cgaacggaca
      181 aaaaacttag cctcccgaat gaccgaacaa tgggcccaag ttcgagttat cgccattaat
      241 ggcgcgatcg aggccaccaa agatccagaa ctgcaaaaaa ggtttcagcg ctatttcgat
      301 gaaattggta aaacggtaac tgaatcacgc gaactctgtt tagagttaga aaaactgggt
      361 agaccagctc cagaattaat ggaacagatc aaagacctcg acgagctttt ggcacgcgta
      421 gcagacgacg caatcgtgtt ttcgccccgg gtttccggcc agaagcgacg ccccgagtaa
      481 gaactgaaga gtattgatga atcgcggtag ctgcaaggcg tgcggttttt ccgcattcat
      541 cgaggggtgg agccaattcg aaaaaaccaa tcgaacgaac tattgagtac tcgccacaaa
      601 ttcgcgccat ttcgatcatt tcaccggcag tgaggccatc aatttgcggc gcactcacac
      661 caggagccca ggccatagag actgcgtcga gatcaaagct aacgacggta gcaatgtttt
      721 tatgcgcaag actcttcaga acttctcgaa aagtagaaat aacgccagat tttgaattac
      781 gcatgcttct caacgtgtga accgtagctc ctcgttttgt gagataggcc tgagcctcct
      841 ttgtgttgca gtgatcctga atgcccaact cgaaaaaaag tttcgggtta aggactcccg
      901 attcgatcgc caggaaaaag ggtgatccac ttgtaatgcc gtgttcattc ggtggccgta
      961 catcgaggtg ggcatcgata tttattaagg ctacatccga ttttttgaaa tcgaccaccc
     1021 cggccaaatg ggggaacccg tagtcgtggc cgccacctag aacaattgga gtgcggtcgc
     1081 ttttcaaact ttgggtgact gcggtgcgca atgccatatg tccttcttcg atcgattttc
     1141 cggctgggac gtcgtaccct ttcagcaaat ttaaagtgtc gagcgacatt ccgatggcca
     1201 tggcttccag gctattgcga attgttgtcg gtccgtgagc agccccgaga cgaccaccca
     1261 cattgcgaac accgcgatca tcgcttgctc caattaattc aattgcgggc aacaaggatc
     1321 ttgattcgga acgttgagat tgttcggttt tgtttttttg cgcagagtca ttgttaactt
     1381 gccagatttc tttgattttt tgcatcttca tagcttcgga ctataattga actcgaccct
     1441 gggaggaata cggaagagaa aaaatggttt ctctatgtcg ttgatcacca cgaaggcccg
     1501 tttagcctga gtgaaattca aagtctggtt aaaaaaggcg acgccaagag ttcaagttat
     1561 gtgtggcgcg aggggtttga agattggatg atgatgtccg aagtttcgga atttggcgta
     1621 gcaggacctt ctggggaagg acacggaccc gcagctttta ttcttggaaa aaaagagagt
     1681 cccgttttgt tttcaccgcg ctcgtctgac gcctctgtcg gcggtccttt accaattgcg
     1741 caccaaggca acgttcccga tatatctgga atctctgaaa ccgccgtggt ctggtgtttg
     1801 aactcaaacc gcactttcac cggacctcat tctttgcgca ccatcacaag attgatcaat
     1861 gagggaaaag ccacatcaaa agatacgatt tggaaagaag gttggactgg atttgttcca
     1921 atttcatcat tctccgaatt tatggttcgc attcacgacg gggcatcaca aaaaaataaa
     1981 gtttcctcag cgcaggcgct gggaatgatg aggtcaggaa tgggcatcaa gcgaaggttt
     2041 tccttcgggg gtattttaaa gtggctagta ttggcatttt tggtgttcgg aggtttttat
     2101 caagtcactg ccatgggttt attgaatccg gttttcgaaa aaattgggat tttagaaact
     2161 gtgcggcgag caaatctaaa gccagttccc gtacctaaaa tcgttattcc ggtggagtac
     2221 ataagtgatg cggggaattt cttgcgaaga caggttgaac cgctcttagc aaaatattca
     2281 gaccatattc cagggccaat tttagagtat ctgtcggtcg tcgaattgcc tgagggatta
     2341 aatgaaaaag atgaagcacg tttacgggat gctgctacag ccaatcccga tgcgggccga
     2401 agagctgccg ccgctttgat tgggcaagag gttatgaatc cgcgatttgt attggcctcg
     2461 aatattgacg ggtcgagttt tcgggtggtt ctgcgaggca aagagggaac cttacttaac
     2521 gcgagtgaat atgtttcgga gcaagtcgta acacttcgaa acagagtagc gatttctcaa
     2581 ccgttctacc ttgaagatac taagtctttg ccccaaggag agtacacatt tacggtgtta
     2641 aatggtgagg agtcggtgtt aattgaaaat gcatttttgg gtggagaaaa cgacagcgca
     2701 tat
//
LOCUS       DAOMXO010000309         2699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21559_length_2699_cov_5.427005, whole genome shotgun sequence.
ACCESSION   DAOMXO010000309 DAOMXO010000000
VERSION     DAOMXO010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21559_length_2699_cov_5.427005"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLH57_07110"
     CDS             complement(<1..697)
                     /locus_tag="PLH57_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HRK02420.1"
                     /translation="MDHPTNEQSGNEKKYWVTPGEGDATLGEFVSTPAKPQDKAEGIA
                     SHDGLGSSERRDFLKLMGASFALMGTAACTRSSVEKIIPYVNKPEEVTPGIANWYASS
                     CTECEAGCGILVKTREARPIKFEGNPKHAASQGGLCARGQASVLNLYDPDRLQAPLAP
                     GRKNLASGTSWEDLDSKVVDKIRAARGKTRILMGETSSPSAWKAVTDFTSQLGAGVPV
                     VWEPQGLDELLNAQ"
     gene            complement(725..1309)
                     /locus_tag="PLH57_07115"
     CDS             complement(725..1309)
                     /locus_tag="PLH57_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRK02421.1"
                     /translation="MKKLLPLVVVVVLIGAGIAALYNVGRNKGYSPDQPIPFSHERHV
                     TQNGIPCMYCHTGVEKTKHATVPAMNVCMNCHSVVLPDSPHIKRLKEAYASGKPIEWV
                     KIHDLPDHVNFNHKRHVAKGIACETCHGDVGQMVKVKQVVDMNMGWCLDCHRGKTTPP
                     EVVSAITKEKAADPVKASMLGTAVAPTNCYTCHH"
     gene            complement(1442..>2699)
                     /locus_tag="PLH57_07120"
     CDS             complement(1442..>2699)
                     /locus_tag="PLH57_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02422.1"
                     /translation="VDHLMDLPASERAAERKRFHSIYASEYAAAAGLVPDFPMSEDEI
                     REQAQRKAAHVFRKKAHRQFDPLRVEDAQLWNQIYTEAYSNARDSIPDFEARSHLRES
                     VIKKAEFLAAHEEHRNEPLKVELDRLRSERRYEEYEALRATQYAQSFVRAYEDSTENE
                     HWDNVGPLSPARPGRWQQFRQRKVVRHQNPLSKSLTIGCRLLEATTNPTHRLGMNALL
                     DRRMWGYYGFKFSVISTARHFPMNASSGYEFRSAVLKTALPRGVWVWYHLITSTMFRM
                     GSSFAYRLLSNIGVKPMGRLSHVFLFSLAYVALTGWGSFFATPSAKWWEMTVWQRVER
                     WWNFAIFPVTWTKHKIKCDIFSSCKKPEAPVKTDAEQVWDTVETPVFSTGDNTGEMDR
                     MWEPSAEDFLMDAAEQWTPIDAATPN"
BASE COUNT          656 a          713 c          627 g          703 t
ORIGIN      
        1 cttgagcatt taataattca tcgagacctt gtggttccca aacaacagga acacccgcgc
       61 ccagttgcga ggtaaaatca gtgactgcct tccaagctga tgggcttgaa gtttccccca
      121 tcagaattcg ggttttgccc ctagcagcac gaattttatc cactacttta gaatctaaat
      181 cttcccaact ggtgccagaa gcaagatttt ttcgacccgg agctagcggc gcttgaagtc
      241 ggtcgggatc ataaagattc aagaccgagg cttgcccccg cgcgcacaat ccaccttgcg
      301 acgctgcatg ctttggattt ccttcaaact taatcggacg agcttcgcgc gtcttaacca
      361 aaattccgca accggcctca cactcggtgc aggacgatgc gtaccaattc gcaattcccg
      421 gagtcacctc ttcgggcttg ttcacgtacg gaataatttt ttcaaccgac gagcgggtac
      481 aggccgcggt tcccatcaag gcaaaactcg cacccatgag cttcaaaaag tctcttcgct
      541 cactactgcc aaggccatcg tgggaagcaa tgccttcggc cttatcctgg ggttttgcgg
      601 gagttgaaac aaactccccc agagtcgcat caccttcccc gggagtaacc caatattttt
      661 tttcgtttcc gctttgttcg ttcgtaggat gatccattga agattccctt tttgatggca
      721 ccaattaatg gtggcacgta taacaattgg ttggagccac tgcggtgccc agcatagatg
      781 ccttcaccgg atcggccgct ttttctttgg taatggcaga aactacttct ggcggggtag
      841 ttttgccgcg atggcaatcc aaacaccaac ccatgttcat atcgaccact tgtttaactt
      901 taaccatctg gccaacgtcg ccgtggcatg tttcgcacgc tatcccttta gcgacgtggc
      961 gcttgtggtt gaaattcaca tgatccggaa gatcgtgaat tttcacccac tcgataggtt
     1021 ttccagaggc ataggcctct ttaagtcttt ttatgtgcgg actgtcgggt aaaactacag
     1081 agtgacaatt catgcaaaca ttcattgccg gaactgtggc atgtttggtt ttttcaaccc
     1141 cagtatgaca atacatgcac ggaatcccat tctgcgtcac atgtctttca tgcgagaatg
     1201 gaattggctg atctggcgaa tagcccttgt ttcggccaac gttataaaga gcggcaatac
     1261 ccgcaccgat caacaccaca acaacaacta gcggtagaag ttttttcatt ttgaactaag
     1321 ttgctccacc taagcagtaa acaaagtttt attggattta gggttcaata ttaaacagtt
     1381 ggtttcaatt ggcaaaaaga aaaagtgagt aaaatgaagt gttactacga ctccgaaggt
     1441 cttagtttgg ggtcgcggcg tctattgggg tccactgttc cgccgcatcc attagaaaat
     1501 cttcggccga gggctcccac atgcggtcca tttcgcccgt gttatcgcca gtactgaaca
     1561 ccggagtttc tacagtatcc cacacctgtt ccgcgtctgt cttaacagga gcttcgggct
     1621 ttttgcagga actgaagata tcgcacttaa ttttatgttt ggtccaggtg actggaaaaa
     1681 tagcgaaatt ccaccagcgc tcaacccgct gccataccgt catttcccac cacttggcgc
     1741 tcggagtcgc aaaaaacgat ccccaacctg taagcgcgac gtaggccagt gaaaacaaga
     1801 acacatggct caaacgaccc atgggtttta cgcctatatt gctcaacaag cggtaggcaa
     1861 aagaacttcc catgcggaac atggttgaag tgattaagtg gtaccaaacc cagactccgc
     1921 gaggaagcgc cgtctttaga accgcggatc gaaactcgta cccggaggac gcattcatcg
     1981 gaaaatggcg ggccgtactt atgactgaga atttaaaccc gtagtacccc cacatacgcc
     2041 gatccaacaa agcgttcatt cctaaacggt gcgttggatt ggtagtggcc tctaaaagtc
     2101 gacaaccgat ggtcagcgac ttagaaagcg ggttctggtg gcgaaccact tttcgctgac
     2161 gaaattgttg ccaacgtcca gggcgggccg ggctcaatgg tcctacgtta tcccaatgtt
     2221 cattttccgt tgagtcctcg taggccctca caaaactctg agcatactgc gtcgctcgaa
     2281 gcgcttcgta ttcttcgtat cgccgctctg aacgcagccg atcaagttcc accttcaaag
     2341 gttcattgcg atgttcttcg tgagccgcca gaaattcagc tttctttatg acgctttctc
     2401 taaggtgcga ccttgcctcg aaatccggaa tactatcacg agcgttggaa taggcttctg
     2461 tatatatttg gttccaaagc tgggcgtctt cgacgcgaag agggtcaaat tggcggtgcg
     2521 cttttttccg aaaaacgtgc gctgctttcc gttgagcttg ctcgcgtatt tcgtcttcac
     2581 tcattgggaa gtctggcact agcccagcag ccgccgcata ttcactggca taaatcgaat
     2641 gaaagcgctt tcgctctgct gcacgctcgc tcgcgggcag atccattaag tgatccacg
//
LOCUS       DAOMXO010000310         2698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21571_length_2698_cov_4.721907, whole genome shotgun sequence.
ACCESSION   DAOMXO010000310 DAOMXO010000000
VERSION     DAOMXO010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21571_length_2698_cov_4.721907"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..838)
                     /locus_tag="PLH57_07125"
     CDS             complement(20..838)
                     /locus_tag="PLH57_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02423.1"
                     /translation="MAPNLVFSFSATILIIVSIFHIGCSPKEMYQKKIAPLFANKPEP
                     SKEPSPEDIPLEHRAINGEFIRELFQVVLERSIASEDEFLRYMNVLDQGGHYEGIYNG
                     VVYSAEFREKEKGNATVSSLKLFAEIMTQITLDQKYDPLRIYPSNDTSEDVPTIKDSN
                     SSPQTPQPSPEERSALVEHFEKEGLTRSLYTLKRRLGEELLKTIDLKKEYREKLATWY
                     GKFSVFLNSQGTDFGIPQRNVKNEYYHYKWGLQADEDRLKWECLNRIHRLMNRR"
     gene            complement(810..1592)
                     /locus_tag="PLH57_07130"
     CDS             complement(810..1592)
                     /locus_tag="PLH57_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02424.1"
                     /translation="MVHLLDIRVLKFSFVILMLGLRTNATAANFDPSTFINATNNLIS
                     ELGAFVGLAADLRAYEGAKPFSSDIGIDFGTSVSAVTVPAKVASALSEIGIQNVPRYI
                     PIPKVNIQKSLPGNLMIGSEFIPTIKVRGYRLEAYGFNLQWTAIDRPRLPPIAIRAQY
                     NYADLVLANAATYGADLVVSHSILFVHMFAGAGYRYVNANVANPTSTLPLNSNVSFDT
                     SIDAGHFFAGTSLVAGFARITAEADFTTRNVNSYGTKLSFFF"
     gene            complement(1602..2495)
                     /locus_tag="PLH57_07135"
     CDS             complement(1602..2495)
                     /locus_tag="PLH57_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK02425.1"
                     /translation="MTEFLFTVASVSTPLVYAALGGMLSERAGVVNIALEGLMLAGAF
                     TAATVAWATKDPWLAFVAGVLASSVFGALHALLTITFRAHAIISGLAVNAVVAGLTPV
                     LCKTLFDSSTSSPMLENAQRITALGGRVSPLVICAPIVALLLYVMHRWSRFGQYIRFA
                     GEVPDALDAQGISSQKVRWLAVCLSGALCGVAGAYLSIDHGAGFTRNMTAGRGYVALA
                     AVIVGRWTPQGALAGALIFGSIEACQILFQDIPLWGDRTLPVQWMQSLPFIATLLVLA
                     VVNRRAAKSPGGLFVPKALSS"
     gene            complement(2492..>2698)
                     /locus_tag="PLH57_07140"
     CDS             complement(2492..>2698)
                     /locus_tag="PLH57_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02426.1"
                     /translation="IGLSVALIARGSLPMLIAATLLFGALQKGALDLDLETERVTRDL
                     AAVIQAVILLVLALQTRKKGGASS"
BASE COUNT          724 a          637 c          680 g          657 t
ORIGIN      
        1 atgatcgaag ctacacaaat taacgtcgat tcatgagacg gtgtatgcga ttgaggcatt
       61 cccatttgag gcgatcctcg tcagcttgaa gtccccattt gtagtggtaa tattcgttct
      121 tcacgtttcg ttggggtatt ccaaaatcgg taccctggga gtttaaaaaa accgaaaatt
      181 taccgtacca ggtggcgagt ttttcgcggt attctttttt aagatcgatg gtcttaagta
      241 gctcttcgcc gagtctacgt ttaagagtgt aaagcgaacg agtgagacct tctttttcga
      301 aatgctcgac gagcgcgcta cgttcctctg gtgaaggttg aggcgtttgc ggtgaactat
      361 tcgagtcttt gatggtcgga acgtcttcgg aagtgtcgtt ggaaggataa atgcgtaatg
      421 ggtcgtactt ttggtccagt gtaatttgag tcatgatttc agcgaatagt ttgagcgaac
      481 ttacggtggc gttgcccttt tccttttctc gaaactcggc agaatagacg accccattgt
      541 aaataccttc gtagtgcccg ccttgatcaa gcacgttcat ataacgtaaa aattcatctt
      601 cgcttgctat cgaacgttcg agaacaactt gaaacaattc tcgaatgaat tcgccgttaa
      661 tagcgcgatg ctcgagcggt atgtcttctg gtgaaggctc tttgctcggt tcaggcttat
      721 tcgcaaatag cggagcaatt tttttttggt acatttcttt aggcgaacat ccgatatgaa
      781 aaatcgaaac gataattaag atagtcgcac taaaagaaaa aactaagttt ggtgccataa
      841 gagttgacgt tcctggtagt aaagtcggcc tctgctgtaa ttcgtgcgaa ccccgcaact
      901 agtgatgttc ctgcaaaaaa gtggcccgcg tcgatgctag tatcaaaact tacattggaa
      961 ttaagtggca aggtgcttgt ggggttcgct acgttcgcgt tgacgtaccg gtaacccgcg
     1021 ccagcgaaca tatggacgaa aagaatcgag tgactcacca ccaagtcggc gccgtaagta
     1081 gcggcattcg ctaggaccag gtccgcgtag ttgtactgcg ctcgaattgc aatgggtgga
     1141 aggcgcggtc ggtcgatagc tgtccattga agattaaatc cgtaagcttc aagtcggtag
     1201 cctcgcacct ttatcgtagg tataaattcg cttccgatca ttaaatttcc cggcaaactt
     1261 ttttgaatat tcaccttggg gattgggatg tagcgtggaa cgttttgaat accgatttca
     1321 ctcaacgcac tggcgacttt tgccggcacg gtaactgctg aaacactggt accaaaatca
     1381 atgccgatat ccgaactaaa cggctttgca ccttcgtacg ctcgtagatc agcggcgagg
     1441 cccacaaacg caccaagttc tgaaattagg ttgttggtgg cattaataaa agtcgaaggg
     1501 tcaaaattag cggccgttgc attggttctt agaccgagca ttaagattac gaaactaaat
     1561 ttcagaacac gaatatcgag gaggtgcacc atgggacaat attaacttga aagagccttg
     1621 ggtacaaata gtccccctgg tgacttggca gccctacgat tgacaactgc gagaaccaaa
     1681 agggtagcga tgaaaggcag actttgcatc cattgcacgg gtagtgttcg gtcgccccag
     1741 agcggaatat cttgaaataa tatctgacac gcttcgatag agccaaaaat caaggcacca
     1801 gctagagcgc cctgtggcgt ccagcgacct acaataacgg ctgcgagggc gacatatccc
     1861 cgacctgctg tcatgttacg agtgaatcct gccccatgat cgatggataa ataggcgccg
     1921 gcgactccac agagcgcccc agataaacac acggctaacc agcgaacttt ttgtgaagaa
     1981 attccttgag cgtcgagtgc gtcgggaact tcaccggcaa aacggatata ctgtccgaaa
     2041 cgggaccaac ggtgcattac atagagcaaa agcgcaacga ttggcgcgca aatcaccagc
     2101 ggtgagacgc ggccaccaag agctgtgatg cgctgcgcat tttcgagcat tggtgaactt
     2161 gtcgaagagt caaacaacgt cttacaaagt accggtgtca aacctgctac gaccgcattc
     2221 acggccaagc cgctgatgat cgcatgagct cgaaaggtta ttgtcaataa tgcgtgtagg
     2281 gcaccaaaaa cggaactggc taagactcct gccacgaatg caagccatgg gtctttagtt
     2341 gcccaggcaa ccgtggcggc cgtaaaggca ccagccaaca tcagaccttc cagtgcaatg
     2401 ttcactacgc ccgcacgttc tgaaagcatc ccgccaagcg cggcgtaaac cagcggcgta
     2461 gacacacttg caacggtaaa cagaaattcg gtcatgacga cgctccacct tttttgcgag
     2521 tttgcagagc gagtaccaat aggattacgg cctgaatcac cgcagcgaga tcacgagtca
     2581 cgcgttcggt ctctaggtca agatctagag cccctttttg aagggcgcca aacagaagcg
     2641 ttgcagcaat cagcattggt aagctgccgc gtgcaattaa ggccacagaa agcccgat
//
LOCUS       DAOMXO010000311         2697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21578_length_2697_cov_5.439440, whole genome shotgun sequence.
ACCESSION   DAOMXO010000311 DAOMXO010000000
VERSION     DAOMXO010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21578_length_2697_cov_5.439440"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..>2697
                     /locus_tag="PLH57_07145"
     CDS             354..>2697
                     /locus_tag="PLH57_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase"
                     /protein_id="HRK02427.1"
                     /translation="MAGKKQTAPTAAHLMDRIVRRALYYSTQMIHLANNRDDVQKGDP
                     KVGGHPAACASSTHILGAIHLSAREPQDFIAVKPHASPMDHSYSYLLSLFYHNRDHAK
                     AEQRFSDDEARAVMRNLRKFMNPADPNHEPAFQSYHAVWDADSFHYFPSGSVGIPPVT
                     AAYTSLAYRFAERHGFDVPKNSHFWSIIGDSEFREGSLAECMPDVAERELNNVTWIVD
                     YNRQNLDGTRIFNEAGFAGTDADRIERMAAANGWRVVQARHGKKRLEFFKKYGDEVKK
                     ALEGLTDFELQALLIKKDGKAFGKRLGELVPKAKSKLQGASSAELLEVLEDLGGHDFA
                     LLSEIFAECRRSKKPTLVVAHTIKGWGLRCQAATGNHSMLPDDDEVVELAQKQGINPE
                     KGDDLFMRFPNNSDESKFLDERGRYLRSGIEAQRALRDQNAEKIKIEIEKEGGFPTSF
                     GINLKLAPWANTQWMWGQLAAKLIRIANLGKAGELSADEKPWNLAGRYLVTLAPDVGT
                     STNLNPSMDGKVFGPDVVDFEALYDVKDKRRPNLVPLEKEANRHLRFEIEEGNAMTCA
                     GAFGKMTDHVGLPILPMMTIYDFFIKRALDQYFYNLYWRSGFILVGTPSGVTLSPEGA
                     QHSWKSDIQIPNGVTWEPAFAIEMDWILADAVRRHFTHDNVDREGVLIRAVTRSIEQK
                     VFIESLREQVRFTGMDDAAILEATRLDVLAGGYWLSHFEGFDGYAPGDNVVHIAVMGA
                     LVPEAVAASKALREQGYFANILVVTSPDLLAGNLAQQNGYD"
BASE COUNT          670 a          709 c          681 g          637 t
ORIGIN      
        1 gttcgtcggt agccagctcg cttcgaagat cttgaacagt ctggttgcac tctttaagaa
       61 gcagcatggg gactttttcg gaaatttctt ggccatcgta ctgaagcatt tgggcaaggg
      121 atggccgccc cggcggcgcc cctgagcccg gcgctggacc actgggtaat ttattcccct
      181 cgggcgcggc ggtaggccct tgagggctcg gcggcccccc tccactactt tttgaagaat
      241 ttccgttact ttccgcgctc aaagaatcat catcctcccc ttctgttctt tcggaatttt
      301 ccgatatata gatgagtcgt tttccctaaa gtctttaaag acgaggaggt ccgatggcgg
      361 gcaaaaagca aactgcgccc actgcggcgc acttgatgga ccggattgtg cgccgagcgc
      421 tgtattattc aactcaaatg attcatctgg ccaacaaccg ggacgacgtc caaaaaggcg
      481 atcccaaagt cggcggacat ccagctgcct gcgccagctc aacccacata ttgggagcca
      541 ttcatctttc agcccgtgag ccacaggatt tcatcgcggt aaaaccccat gcaagtccta
      601 tggaccattc ctattcctat ttgctttccc ttttttatca caaccgcgac cacgccaagg
      661 cagagcaacg ttttagcgac gacgaagctc gcgcggtcat gcgcaatcta agaaagttca
      721 tgaatccagc tgatcccaat cacgaaccag cgtttcaatc ttatcacgca gtgtgggacg
      781 cggattcgtt ccactatttt ccatcgggtt cagtgggcat tccacctgta acggcggcct
      841 acacttcact tgcttatcga tttgccgaac gccacggttt cgatgttccc aaaaattctc
      901 atttttggtc gatcatcggg gactccgagt ttcgcgaggg aagccttgca gagtgcatgc
      961 ctgatgtagc cgaacgcgaa ttgaataacg tcacttggat cgtcgattat aaccgtcaga
     1021 acctcgacgg tactcgaatt ttcaatgagg cgggattcgc aggcacagat gccgaccgta
     1081 ttgagcgcat ggctgcggcc aacggctggc gagttgtcca agcgcgccac ggaaaaaagc
     1141 gtttggagtt ttttaaaaaa tatggcgacg aagttaaaaa ggcactcgaa ggtctcactg
     1201 attttgaatt acaggcgctt ttaattaaaa aagacggcaa ggcctttgga aaacgtcttg
     1261 gtgaattggt accaaaagca aagagcaagc ttcaaggtgc aagttccgcg gaacttctcg
     1321 aagtactcga agatttaggt ggacacgact tcgcgctact tagcgaaatt ttcgccgagt
     1381 gccgccgttc caaaaaacca acgctcgtgg ttgctcacac gatcaaaggt tggggtcttc
     1441 gttgccaggc ggcaactgga aaccatagca tgctcccaga cgacgacgaa gttgttgaat
     1501 tagcgcaaaa acagggaatc aatcctgaaa aaggcgacga cctttttatg cgattcccta
     1561 acaacagcga tgagtcaaag tttctagacg agcgcggccg ttatttacgc tcgggcatcg
     1621 aagcccaacg cgctcttcgc gatcaaaacg cagagaaaat aaaaattgaa atcgaaaaag
     1681 aaggcggttt tccaacttcc tttggcatca atctcaagct cgcgccctgg gctaataccc
     1741 agtggatgtg gggtcaactg gctgctaaac ttattcgaat tgccaacctc ggaaaagctg
     1801 gcgaactcag cgcggacgaa aagccttgga atttagctgg gcgctatctc gtcactctcg
     1861 ccccagacgt gggcactagt accaacttaa atccgagcat ggacggcaag gtattcgggc
     1921 ccgacgtggt cgattttgaa gcgctttacg acgtaaaaga taaacgccga cctaatcttg
     1981 taccacttga aaaagaagcc aatcggcacc tgcgctttga aatcgaagaa ggcaatgcca
     2041 tgacctgcgc cggtgcattt ggcaaaatga ctgaccacgt gggcttacct attcttccca
     2101 tgatgacgat ctacgacttt ttcataaagc gcgcgctcga ccagtatttt tacaatctct
     2161 attggcgcag tggctttatc ttagtgggca cgccgtctgg cgtaacactc tctccagaag
     2221 gtgctcaaca cagctggaag tctgacattc aaattccaaa cggagtaacc tgggaacccg
     2281 cattcgccat cgaaatggac tggatcttgg ccgacgcggt acgacgtcac ttcacgcacg
     2341 acaacgtcga tcgcgaggga gtgctgatcc gggcggttac gcgttcaatc gagcaaaagg
     2401 tttttatcga aagcctaaga gagcaggttc gattcaccgg catggacgac gctgcgatac
     2461 tcgaagcgac ccgactagat gttttggccg gtggttattg gctgagtcac tttgaaggct
     2521 tcgatggtta tgccccaggg gacaacgtcg ttcacattgc agtcatgggt gcattggttc
     2581 ccgaggctgt cgccgcatct aaggctctgc gcgagcaggg gtattttgcg aacattctgg
     2641 tcgtcacctc acctgacctt cttgctggaa accttgccca gcaaaacggt tacgacc
//
LOCUS       DAOMXO010000312         2692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21627_length_2692_cov_4.182404, whole genome shotgun sequence.
ACCESSION   DAOMXO010000312 DAOMXO010000000
VERSION     DAOMXO010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21627_length_2692_cov_4.182404"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PLH57_07150"
     CDS             complement(<1..980)
                     /locus_tag="PLH57_07150"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF020460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRK02428.1"
                     /translation="MSESNHTSLPTTNSPASLVTPCPPGNAYVDCVRTRLKNYLEEQP
                     LTKWIEARAAGGVEPFYTSIDLRDAGFKLAPVDANLFPAGFNNICPEDLFHSAPIARR
                     VLIKHLGRVPKNLVILPESHTKNRFYVDNLLALKQIFENLGIVVHIGWWGEIPTESLV
                     LKDGIETVRLSSSTGTEIFAVPLYLKNEQLVLKDLTLEAPAEIAPEFILLNNDFSSGF
                     PLKLSCVSQPIEPSFRLGWHTRKKHEFFENYNRLAGELALNAKLDPWHLQVDTELVTD
                     VNFDESVGMERIAAAAERVLNRIRLEYEKRGIEEKPFVFIKSNSGTYGMG"
     gene            complement(984..2051)
                     /locus_tag="PLH57_07155"
     CDS             complement(984..2051)
                     /locus_tag="PLH57_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02429.1"
                     /translation="MNQNKSAPRVFRFPIFLILFIFAGCSLRTASREDLSPTTETIPT
                     TQAIAVPTENEALYLNAVRLMSQAAFGEAALKLEQYLAANTLAPNRGPALLNLALCQL
                     HLDHSDAALQSTRTLLEDEAYFATKDIQVQARWIRAEAFLRKNRPAEALALTFELLPS
                     RSTKSKPSGFDVRGNWSGKLEPLEIAKTLALRGRIYGDLGKTALAEAALARAVKALAK
                     GPESERRALRAQVASYRIEIIEKTCAIQHPAPSPLSEREAIAYIEDYYACARAARAQY
                     CEVKAHNDQAILSRSLISYRRLASAPLQLIRQLPPPARPVRPEERNHYEKELKAAIES
                     WSEEKMKTFRNLDSCGAENIF"
     gene            complement(2048..>2692)
                     /locus_tag="PLH57_07160"
     CDS             complement(2048..>2692)
                     /locus_tag="PLH57_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02430.1"
                     /translation="APNAVAFLKAPGQLARFFDDSLMKWFIAPGSKLLAAGIKRILEI
                     LERLTGKGFISELVEFTAALFELKVQFIANLTEIEAVLRSSSTSFIMVTTPERYNSAD
                     TTDFVEHLVREHYPFWGFLVNRSLTARLGIANKASLATIGPVAACSEQEQNFLNALFD
                     RQRSRIEKEFTVFESCKKKNANVATVQAFDQDIHNVDDLADYASKISSIGAPSP"
BASE COUNT          669 a          614 c          692 g          717 t
ORIGIN      
        1 atacccatgc cgtaggtgcc agaattgctt ttaataaata cgaagggctt ttcttcgatg
       61 ccgcgttttt catattctaa acggatgcga tttaaaaccc gttccgctgc tgctgcaatg
      121 cgttccattc cgacggactc gtcgaaatta acgtcggtca cgagttcagt atctacctga
      181 aggtgccagg ggtcgagctt ggcgttgagc gcaagttctc cagctaaacg gttgtagttt
      241 tcaaaaaact catgtttctt gcgagtgtgc caacccaatc gaaagctcgg ttcgatgggt
      301 tgtgaaacgc aagaaagttt tagcggaaac cctgaagaaa agtcgttatt gagcaatata
      361 aactctggcg caatttcggc cggagcctcg agcgtgaggt ccttcaaaac tagttgttca
      421 ttttttaaat acaaaggcac cgcaaatatt tcggtaccag tcgacgagct gagccgtacg
      481 gtttcgattc catctttaag aacgagcgat tcggtaggga tctcacccca ccagccgatg
      541 tgaactacaa tgcccaaatt ttcaaaaatt tgcttaagtg ccagcaggtt gtcgacgtag
      601 aagcggttct tagtgtggct ctccggtaga atcaccaagt tttttggtac gcggcccaag
      661 tgcttaatta aaacgcgccg cgcaatcgga gcagagtgaa aaagatcttc ggggcaaatg
      721 ttattgaaac cggccggaaa gagattggca tcgaccggtg cgagtttaaa gccagcatca
      781 cgaaggtcga ttgaagtgta aaaaggctcc acaccaccag cggccctagc ttcgatccat
      841 ttggtcagcg gttgttcttc gagataattt ttgagtcgag tgcgcacaca atcgacatag
      901 gcattgcccg gcggacaggg cgtgacgagt gaggccggcg aattagtagt gggcaaagac
      961 gtgtggttgg attcgctcat tagttaaaag atgttctctg caccgcaact gtcaaggttg
     1021 cgaaatgttt tcatcttctc ctcggaccaa gactcaatag cagccttgag ttccttttcg
     1081 tagtgatttc gctcctctgg ccttaccggt cgagccggtg gtggtaactg ccgaatcaat
     1141 tgaagcggcg cgctagctag tcgtcgataa gagatcaaag atcttgaaag tatcgcttgg
     1201 tcgttatggg cctttacctc gcaatattgc gctcgtgcgg cccgagcaca agcgtaatag
     1261 tcttctatat acgcaatcgc ttcgcgttcc gatagggggc tcggtgcagg atgttgaatt
     1321 gcgcaggttt tttcaatgat ttcaatgcga taggatgcaa cttgggcgcg caacgctcgt
     1381 cgctcggatt caggcccttt tgctaaagcc tttactgccc gagccagcgc tgcttcagcc
     1441 aatgcggttt tgcccaagtc tccgtaaata cgacccctca atgccagggt tttggcaatt
     1501 tcgagtggtt cgagttttcc gctccaattc ccacgtacat caaaccccga tggttttgat
     1561 ttggttgacc gacttggaag tagctcaaat gtgagtgcga gggcttccgc cgggcgattt
     1621 ttgcgtaaga atgcttcagc tcgaatccaa cgagcctgaa cttgaatgtc cttagtcgca
     1681 aaataggctt cgtcttcgag cagagtacgg gtgctctgta gggccgcatc ggagtggtcg
     1741 aggtgcagct ggcagagagc caaatttaac aacgcgggac cacgatttgg tgcaagtgta
     1801 ttggcggcca ggtattgttc cagttttaac gctgcttcac caaatgccgc ctgactcatg
     1861 agacgtacgg cgtttaagta tagtgcttcg ttttctgtag gtaccgcaat agcctgggta
     1921 gttggtattg tttcggtcgt cggtgaaaga tcttcccggc tcgctgttcg caaggagcaa
     1981 cccgcaaaaa taaataaaat taaaaagatt ggaaaacgaa agacccgggg ggccgatttg
     2041 ttttggttca tggtgatgga gctccgattg acgaaatttt agatgcgtaa tcagctaagt
     2101 catccacatt gtggatatct tgatcgaatg cctggacggt ggccacatta gcgttctttt
     2161 ttttgcagct ttcaaaaact gtaaactctt tttcgattcg actacgctga cggtcaaaaa
     2221 gcgcgtttaa aaaattctgt tcctgttccg aacaggcggc tacaggaccg atagttgcga
     2281 gcgatgcttt gttggcaatt ccaagtcgcg cggtcaagct gcggtttact aaaaaacccc
     2341 aaaacgggta atgctctcga accagatgtt ctacgaagtc ggtagtgtcg gccgaattgt
     2401 accgctcggg ggtggtgacc attataaaac tagtggaaga agatctaagc acagcttcga
     2461 tttcagtgag atttgcgatg aattgcactt tgagttcaaa aagggctgcg gtgaactcta
     2521 caagttcgct aataaaacct ttaccagtaa ggcgttcaag tatttcgaga attcttttta
     2581 tccccgccgc taaaagtttg ctacctggtg cgataaacca cttcattaaa ctgtcgtcga
     2641 aaaaacgagc caactgacct ggggctttca aaaatgcaac ggcgttgggt gc
//
LOCUS       DAOMXO010000313         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21730_length_2683_cov_6.601218, whole genome shotgun sequence.
ACCESSION   DAOMXO010000313 DAOMXO010000000
VERSION     DAOMXO010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21730_length_2683_cov_6.601218"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLH57_07165"
     CDS             complement(<1..727)
                     /locus_tag="PLH57_07165"
                     /inference="COORDINATES: protein motif:HMM:NF026047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRK02431.1"
                     /translation="MTEQHNPCGIRGIEFVEFSSNQPESLRALFLDFGFARTLRHQKK
                     KVDFYVQNDIHFLVNTEANSHAQTFCKMHGPCISSMGWRVDNAKEALAEAVKRGAKAC
                     TKPDYERAGSQVPAIYGIGDSLIYFIDRFSDKALYSELGFRPVEGADPQNDKGFLLID
                     HLTNNVEKGTMSKWSDFYKSVFGFTEIRYFDIRGTQTGLTSFALKSPDGSFCIPINEG
                     TEAKSQINEYLREYNGPGIQHLAF"
     gene            849..>2683
                     /gene="typA"
                     /locus_tag="PLH57_07170"
     CDS             849..>2683
                     /gene="typA"
                     /locus_tag="PLH57_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRK02432.1"
                     /translation="MITNKPELIRNIATIAHVDHGKTTLVDGLLRQAGAFASHVQVAE
                     RVMDSMDLERERGITIQAKNAAVQYNDCKINIVDTPGHADFGGEVERIMGMVDGALLL
                     VDAAEGPLPQTRFVLSKALKQGLRIILIINKVDRPDARVDEVINSTFDLFMDLGASET
                     QADFPIVYACARQGWCTLKSEEVPALLESKGQGGSLKPLFDTILTTVPAPPADSAGTF
                     QMLVSNLAYSDYLGRLAIGRVTRGTIKKNQRMMRLGENGKTESFNLSALYVYSGPVQV
                     EVPEVGAGDIAVFAGNEDVQIGDTLVQLPAQGEKPEPLPRILVDPPTISMVFSVNTSP
                     LSGKEGEPIQSRKLKERLERETRANVALRMESTDSSEQYRVLGRGELQLAILIEQMRR
                     EGYEFMVGKPMVVVKEEGGQKLEPFERAILDLPQAYVGAVTDLFQTRKGQLLEYRTEH
                     EGRVKLEFVIPTRGLIGIRSRYLTETRGEGMFNTQFEGYQPWKGVLLHRMCGALISDR
                     LGDAVEYGLMGLEDRGVLFVKPGVPVYEGMVIGEHNRDNDLNVNPCREKKLTNIRSAH
                     AEVLVNLRGTRDMSLEQCLEWIDEDEWVEITPKSVRIRKKVLESNR"
BASE COUNT          653 a          665 c          722 g          643 t
ORIGIN      
        1 aaaacgccaa atgctgaatt ccagggccgt tgtattcacg gagatattca ttgatctggg
       61 actttgcctc cgtgccttcg ttgatcggaa tgcaaaagga cccgtccggc gattttaaag
      121 cgaaactggt aagccccgtt tgggtgccac ggatatcgaa ataacgtatt tcggtgaatc
      181 cgaacacgct cttatagaaa tcgctccact tgctcatcgt acctttttct acgttgttgg
      241 ttagatggtc gatcagtaga aaacccttgt cgttttgcgg gtcggcgcct tcgacgggtc
      301 gaaaccctaa ttccgaatag agcgctttat cgctgaagcg gtcgatgaag tagattaaac
      361 tatcgccgat cccatagatt gcgggtactt ggctgccggc ccgttcatag tctggtttgg
      421 tgcaggcctt tgccccccgt ttcacagctt cggctagggc ctctttggca ttgtccactc
      481 gccagcccat ggaactaatg cagggaccat gcattttgca aaatgtttgg gcgtgggaat
      541 tggcctcggt gttgaccaga aaatggatgt cgttttgaac gtagaaatcc accttctttt
      601 tctggtggcg cagagtgcgg gcaaaaccaa agtctaagaa caaggcccgt aaggactccg
      661 gctggtttga tgagaattct acgaactcga tgcctcgaat tccgcatgga ttgtgttgtt
      721 cagtcatcgt aaagtctcca gattttggga tcaggctaag ccgcataggc cagcctgaca
      781 atcgattttt ccgccaaaaa aataggtcgg acagtgcaac gcaactgtga tatcgtcggt
      841 gcacctaaat gattaccaat aaaccagaac ttatacggaa tatagccacc attgcccacg
      901 tcgaccacgg taaaaccacc ttggtagacg gcttgcttcg ccaagcgggc gcgttcgcca
      961 gccacgttca ggttgctgaa cgagttatgg actccatgga tctcgaacgc gaacgcggca
     1021 ttaccattca agccaagaat gccgccgttc agtacaacga ctgtaaaatc aatatcgtcg
     1081 acaccccggg ccacgccgat tttggtgggg aagttgaacg tatcatgggc atggtcgacg
     1141 gcgcactttt gctagtcgat gcggccgaag gcccgcttcc gcaaacgcgg ttcgtgttat
     1201 cgaaggccct taagcagggt ctgcgtatta ttttgatcat taataaggtc gatcgccccg
     1261 acgcgcgggt agacgaagtc atcaactcca cgtttgatct ctttatggat ctgggcgcca
     1321 gcgaaaccca ggctgatttt ccaatcgtat atgcttgtgc acggcagggt tggtgcactc
     1381 ttaaatctga agaagttccg gcgttgcttg agagcaaggg ccaaggcggc tcactcaagc
     1441 cgcttttcga taccatcttg acgaccgtac cagcaccacc agccgatagc gcgggcacgt
     1501 ttcaaatgct cgttagtaat ttggcctata gcgattactt agggcgccta gcaattggtc
     1561 gtgtcacacg cggaactatc aaaaaaaacc agcgcatgat gcgtcttgga gaaaacggca
     1621 aaactgagtc gtttaacctt tctgcgttgt acgtttactc agggccggtg caggtagaag
     1681 ttccagaagt cggagctggc gacatcgccg tgttcgccgg taatgaagac gtacaaatcg
     1741 gagacaccct ggttcaatta ccagctcaag gtgaaaagcc tgagccgctg cctcgcattt
     1801 tggtagatcc accgacgatt agcatggttt tttcggtcaa cactagccct ttgtctggca
     1861 aagagggtga accaattcag tctcgtaaac ttaaagaacg ccttgagcgc gagactcggg
     1921 ccaacgtcgc cctccgcatg gaaagcaccg acagcagtga acaataccgg gtgctcggac
     1981 ggggtgaact tcaactggct attcttatcg agcagatgcg ccgagaaggc tacgagttca
     2041 tggttggcaa acccatggtg gtggtgaagg aagagggcgg tcagaaactt gagccgtttg
     2101 aacgcgctat tctagatttg cctcaggcat acgtgggtgc ggtgaccgat ttgtttcaga
     2161 ctcgaaaagg tcaactgctc gaatatcgta cagagcacga aggtcgcgtg aagttggagt
     2221 tcgtaattcc aactcgtgga ctgattggca ttcgctcgcg ttatctcacg gagactcgcg
     2281 gtgaaggaat gttcaatacc cagttcgaag gctaccagcc gtggaagggc gtgctattgc
     2341 accgcatgtg cggcgccctg attagcgacc gcttaggaga cgcagtagag tacggcctta
     2401 tgggcctcga agatcgcgga gtgttgttcg ttaaacctgg ggttcccgtt tacgaaggca
     2461 tggtcatcgg agaacacaat cgcgacaacg atttgaatgt aaacccttgc cgcgaaaaga
     2521 agctcaccaa cattcgctcg gctcacgccg aagttttggt taacttgcga ggcactcgcg
     2581 acatgtctct tgaacagtgc ctagagtgga ttgacgaaga cgagtgggtg gaaatcactc
     2641 cgaagtcggt tcgcattcgc aaaaaagtgc tcgaaagcaa tcg
//
LOCUS       DAOMXO010000314         2675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21838_length_2675_cov_6.236641, whole genome shotgun sequence.
ACCESSION   DAOMXO010000314 DAOMXO010000000
VERSION     DAOMXO010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21838_length_2675_cov_6.236641"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PLH57_07175"
     CDS             complement(<1..806)
                     /locus_tag="PLH57_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRK02433.1"
                     /translation="MKKKDLIFFGMFGFLSMTLALVVFEAGLRIFSVVPTVDKQYSAF
                     RVDSEISYGHSPNTVSAGTAPYDEFVYEYRHNSQGFRDIEHPEKKEPGTFRILGLGDS
                     FTYGAGAQFEDTYLAVVERELNNNNLNKRVEIIKAGISRSFPEHQRLVLKKYGPYYRP
                     DLVIVGFTPNDIVDTHLSLNSIQVRPDGRLQIGSREPSVINDFLYRNSHVWRFIRFTI
                     FRFSQRLHWKLMYDVEENKDAWKRILADYDDMAKLSRDLGAQMMIVVIPQS"
     gene            complement(822..974)
                     /locus_tag="PLH57_07180"
     CDS             complement(822..974)
                     /locus_tag="PLH57_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HRK02434.1"
                     /translation="MYDLFKDFWQFTCERKKFFLLPVVVMLALLGAILVFSQGSAVAP
                     FIYTLF"
     gene            complement(974..1363)
                     /locus_tag="PLH57_07185"
     CDS             complement(974..1363)
                     /locus_tag="PLH57_07185"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HRK02435.1"
                     /translation="MTETTNATLKHFGLLVGGVFFAVFGLIAPLIKGSTVSVWALAIG
                     GLLLILAVTAPKALAIPYRSWMILGELLGWINSRIILGAVFLLVLTPIGILRRIFSPS
                     HHRTSTEKNTYRIQSLPRTSQSLERPF"
     gene            complement(1356..>2675)
                     /locus_tag="PLH57_07190"
     CDS             complement(1356..>2675)
                     /locus_tag="PLH57_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HRK02436.1"
                     /translation="SGRAGWPVGVRAYARRFTYYTGFKVNSGEYKVMGLAPYGEPKYV
                     REILDHLIDLKEDGTFELDMSYFDYATGLKMINEKFCRLFGGPARKSDAPVTQKEMDL
                     ARSIQFVTEEIVLRLATTLQKETNTDSLCLAGGVALNCVANGRLLREGPFKHIWIQPA
                     AGDAGGALGAALVGWHTYLGNPRLVSDAQQDSMHGGYLGPDFTNNEIKNKLNQLGAVY
                     EELDAHQIPTAVAKILEKENVVGWFQGRMEFGPRALGARSILGDARSARMQSTMNLKI
                     KYRESFRPFAPAVLEERVSEYFNYNGESPYMLMVSSVKEAIRTPMSPEQKKLFGIDKL
                     HVPRSSIPAVTHVDYSARIQTVSARTNPRFHSLLKEFDRATGCPVLVNTSFNVRGEPI
                     VCTPEDAYRCFMRTEMDFLVVENFILEKTRQPTHANDDLWKKEFELD"
BASE COUNT          709 a          626 c          630 g          710 t
ORIGIN      
        1 gattgaggga ttacaacaat catcatttgg gcgccaagat ctctgcttag tttggccata
       61 tcgtcgtaat ccgctaggat gcgtttccac gcgtctttgt tttcctcgac gtcatacatt
      121 aacttccaat gaagccgctg agaaaaacga aaaatggtga atcggataaa tcgccacaca
      181 tgggaattgc gatagagaaa atcgtttata acagacggtt cccgcgaacc gatttgcaat
      241 cgaccgtcag gacgtacctg aatactgtta agtgacaagt gcgtgtcaac gatgtcgttg
      301 ggtgtaaatc ctacgatcac taggtctggg cggtagtaag gtccgtattt ttttagaacg
      361 agtctttgat gttctgggaa actgcgagaa atgccagctt tgattatttc tacccgtttg
      421 ttcaggttat tgttatttag ctctcgttcg actactgcaa gataggtgtc ttcgaattga
      481 gcccccgcgc cgtaagtgaa gctatcgcct aatccgagaa tgcgaaatgt tcctggctcc
      541 tttttttcag gatgctcgat atctcggaag ccctggctgt tgtgtcggta ttcataaacg
      601 aattcgtcgt acggagcggt acccgcagaa acggtattcg gagagtggcc gtagctgatt
      661 tctgaatcaa cacgaaatgc gctgtattgt ttatcaactg tggggaccac tgaaaatatt
      721 cgtagaccag cctcgaaaac gaccaaagcc agagtcatag acaaaaagcc gaacattcca
      781 aaaaatatta agtctttttt cttcatcgcg gattccgaac tctaaaacag agtgtaaatg
      841 aacggcgcga ctgctgaccc ttgcgagaaa accagtattg cgcccaacaa ggcaagcatt
      901 acaactacag gaaggagaaa aaatttcttg cgttcgcagg tgaattgcca aaagtcttta
      961 aatagatcgt acattagaat ggcctctcga gagattgtga agtgcggggt agtgattgta
     1021 tgcggtaggt atttttctct gtgctagttc tgtggtgtga cggtgagaat attcgtctta
     1081 aaatcccaat tggagttaac accagcagaa aaaccgcacc aagaatgatg cgggaattga
     1141 tccacccaag tagctcaccc aagatcatcc agcttcgata cgggattgcc aaagcttttg
     1201 gagcagttac cgccaaaatt agtaacaagc cgccaatcgc cagcgcccaa accgaaacgg
     1261 tgctgccttt tatgagaggt gcaatcaatc caaaaaccgc aaaaaacact ccaccgacca
     1321 aaagtccaaa gtgctttagc gttgcgttcg tggtctcagt caagctcgaa ctcctttttc
     1381 caaagatcgt cgttggcatg tgttggctga cgagtttttt ccaatatgaa attttcgacc
     1441 accaaaaagt ccatttcagt tctcataaaa caacggtagg cgtcttcggg ggtacaaaca
     1501 attggctcac cacgcacatt aaacgaagtg ttgacgagca ccgggcaacc ggtagcgcgg
     1561 tcaaactctt ttagtagcga atgaaatctg ggattggtac gagcgcttac tgtttgaatc
     1621 cgtgcagagt aatctacgtg ggtaaccgct ggaattgaag agcgcggtac gtggagcttg
     1681 tcgattccga aaagtttttt ctgttctggt gacatcggag tacgaattgc ttccttcacg
     1741 ctagaaacca tgagcatgta ggggctctca ccattgtaat taaagtattc gcttactctt
     1801 tcttctaaga ctgccggagc aaatggccga aaagattcgc ggtatttaat ttttagattc
     1861 atcgtagatt gcattcgcgc actacgcgca tctcctagaa tcgatctcgc tccgagagcc
     1921 cgtggtccaa attccatgcg gccttggaac cagcccacaa cattttcttt ttccaaaatt
     1981 tttgcaacgg ctgttggaat ttggtgagca tctaattctt cgtagaccgc ccctagttga
     2041 ttgagcttgt tcttgatttc attatttgtg aagtcggggc ccaagtaacc gccatgcata
     2101 gaatcttgtt gagcgtcaga cactaagcgc gggtttccga gataggtgtg ccacccaacg
     2161 agggcggctc ctaaagcacc gccagcgtcc cctgcggcag gttgaatcca aatatgctta
     2221 aaaggccctt cacgtagaag tcgaccgttt gcgacgcagt tgagggcgac acctccggct
     2281 agacaaagac tgtcagtgtt ggtttccttc tgcaaagtgg tcgcaaggcg gaggactatt
     2341 tcctcagtta cgaactgaat cgagcgtgcc agatccattt ctttttgagt gaccggtgca
     2401 tcgctctttc gtgcggggcc cccgaaaaga cggcaaaatt tttcgttgat cattttaagg
     2461 ccggtcgcgt aatcaaaata gctcatatcg agctcgaaag tgccgtcttc cttcaagtcg
     2521 atcagatgat ccaatatttc acgaacatac ttaggctctc cgtaaggagc gagtcccata
     2581 accttgtatt cgccggaatt aactttgaac cctgtgtaat aagtgaagcg ccgtgcatac
     2641 gctcgaactc ctacaggcca acctgctctc cccga
//
LOCUS       DAOMXO010000315         2670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21897_length_2670_cov_6.123518, whole genome shotgun sequence.
ACCESSION   DAOMXO010000315 DAOMXO010000000
VERSION     DAOMXO010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21897_length_2670_cov_6.123518"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..597
                     /locus_tag="PLH57_07195"
     CDS             106..597
                     /locus_tag="PLH57_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02437.1"
                     /translation="MKRNSVFLLIATLAVAVVSAACGRDPRSTQMREVVNSCKENNLS
                     KFKGQFSGAAHKQYANPNGFNQICGMINGALDGSVSAMDGGADVLPPSARLTIQETAV
                     AAGKSKSKTRYTIKEIPGLALVQTCEQSCAPGAGDGGTKCDPVLCDITEVDFNDTTVS
                     YSN"
     gene            complement(589..1533)
                     /locus_tag="PLH57_07200"
     CDS             complement(589..1533)
                     /locus_tag="PLH57_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02438.1"
                     /translation="MSVTASPLFHLSEAMTTLSCPGKVFIFGEYACLVGGPAILMPCS
                     PVFSLQYEFPVAPNVANALNIFAAESPAGRLMRENQAFAETLKLHWQDPYATPIGVGS
                     SSAQFVLLSEVLAQIQIKPSSLTAQQIAGLLQNYWRIVDKSQGLRPSGVDLALQAFGK
                     PCVVVNEPFSIRELRPDPLQPGQMFVLAYSGHKEKTHDHLLDLQRRGFPDAYQSALRE
                     LTVITETALTAWERREANQLGLLLNEYQTTLEKMGLVPAPFANKIAALQKIEGVVGCK
                     GAGAQGGDCALLVIEKSSIEKIKRALHPLGFELANALI"
     gene            complement(1496..>2670)
                     /locus_tag="PLH57_07205"
     CDS             complement(1496..>2670)
                     /locus_tag="PLH57_07205"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HRK02439.1"
                     /translation="HMIENVVGVFSLPLGIATNFLIDERDVPIPMAVEETSIVAAASA
                     AAKWIRRNGGFHTRAIGRLIIGQIQFPSITAPEAFEEKVNAAAEDLIDTANQVVPNLV
                     SRGGGVREIKVRLLERPDQRTMAVLHVLCDPCDAMGANLINQICEGLKKPLEVLTGEQ
                     VGLCILSNLVDTKMVEATCEIRDIEPTLGRAIEEATLFANLDPYRCATHNKGVMNGID
                     PILIATGNDWRAVEAGIHAYVALGGEFRGARPAAFQQDYRSITHWEYTGDVLKGRLLA
                     PIVVGTVGGVTKIHPTANTCLKMLGVSKAEDLARICAAVGLGQNFAALRALCGEGIVR
                     GHMRLHATNLAIAAGAAGHEVATVEERLRKETRITLSRAKEILSDVRDSVPAVPPV"
BASE COUNT          667 a          658 c          686 g          659 t
ORIGIN      
        1 cctaggccca ttaaacccga caaaaaaagt ttacccccac cttgcttaga ttaacgcgta
       61 gtgttatagc agtacacgac ttttgggtat tttaaggagg tagctatgaa aagaaattcg
      121 gtatttttgt taatcgcaac tttagcggtg gcggtagttt ccgcggcctg cggtcgagac
      181 cctcgttcga cgcaaatgcg agaagttgtg aattcctgta aagagaacaa tcttagcaag
      241 ttcaaaggcc agttttctgg tgcggctcat aaacagtatg cgaatccaaa tgggtttaat
      301 cagatctgcg gaatgatcaa cggcgccctc gatggttcgg taagtgcgat ggacggtgga
      361 gctgatgttc ttcctcctag cgcgcgcctt acgattcaag aaactgcggt tgccgctggt
      421 aaatcaaaaa gtaaaactcg ctacaccatc aaagagattc cgggtttagc tttggttcaa
      481 acttgtgaac agagctgtgc tccaggtgcc ggagacggtg gaacgaaatg cgatccagtt
      541 ttgtgtgata ttactgaagt cgattttaac gacacgactg taagttactc aaattaatgc
      601 attagcgagc tcgaaaccaa gagggtgtag agctcgctta attttttcga tcgagctttt
      661 ttcgataacc agtagtgcgc agtcgccacc ttgagcaccg gcgcccttgc accccaccac
      721 tccttcgatt ttttgcagcg ccgctatttt gttcgcaaat ggcgctggaa ctaaacccat
      781 tttttctaat gtggtttgat attcattcaa tagcagtccc aattggttcg cttcacgtcg
      841 ctcccaagca gtgagtgcgg tttcagtgat gactgtaagc tcgcggaggg cgctttggta
      901 ggcgtccggg aacccccgac gttgaaggtc taaaaggtga tcgtgagttt tttctttgtg
      961 acctgaatag gccaatacaa acatttggcc cggctgcaaa gggtcaggtc gaagttcccg
     1021 gatggaaaac ggttcattca ccacgacgca tggctttcca aaggcctgga gagcgagatc
     1081 gactcctgaa gggcgcaaac cctgcgactt atctacgatt cgccagtaat tttgtaaaag
     1141 ccccgcaatc tgctgggcgg taagcgaact gggtttaatt tgaatttggg cgagtacttc
     1201 gcttagcaag acaaattgtg cagaagacga accgactccg ataggcgtgg cataggggtc
     1261 ttgccagtgg agttttaatg tttcggcaaa cgcttggttt tcacgcatca gacgaccggc
     1321 tggcgactct gcggcaaaaa tatttagggc gttggcaaca ttaggagcta ccggaaactc
     1381 atattggaga ctaaaaacag gtgaacacgg cattaatatc gctggtccgc caacgagaca
     1441 ggcgtactct ccaaatataa acactttgcc ggggcaactg agcgtcgtca tggcttcaga
     1501 caggtggaac agcggggacg ctgtcacgga catcggacaa tatttctttg gcacgggaaa
     1561 gagtgatacg agtttcttta cgcaatcgct cttcaacggt ggccacttcg tgccccgcag
     1621 ccccagcggc aatggctaga ttagtcgcgt gcaaccgcat atgtccacgt acaatgcctt
     1681 caccgcaaag cgcccgcaga gccgcaaaat tttgaccaag gccaaccgca gcgcatatgc
     1741 gagcaaggtc ttcagctttc gaaactccaa gcatttttaa gcaagtattc gccgtgggat
     1801 ggatcttagt aacaccaccc acggtgccca cgactatagg agctaacagg cgacccttga
     1861 ggacatcgcc agtgtattcc caatgggtaa tagatcggta gtcttgttga aaggccgccg
     1921 gacgggctcc gcgaaattca cccccaagcg caacgtatgc atgaattccg gcttcgacag
     1981 ctcgccagtc attgccggta gcaattaaaa tcgggtcaat tccgttcatc acacctttat
     2041 tgtgggtggc acaacgataa gggtcgaggt ttgcgaagag ggtcgcctct tcaattgctc
     2101 ggcccagagt gggttcaata tcgcgaattt cacaggtggc ttcgaccatt ttggtgtcga
     2161 ccagattcga aagaatacag agcccgacct gttcaccagt caagacttcg aggggctttt
     2221 ttaagccttc gcagatttgg ttaatgagat ttgcgcccat cgcgtcgcac gggtcgcaca
     2281 acacgtgcaa gacagccatg gttcgttggt ctggccgttc aagaagccga accttgattt
     2341 ctcgtactcc tccgcctcgg gaaacaagat taggtactac ttggttggcg gtgtcgatta
     2401 aatcttcagc ggccgcgttc accttttctt caaaagcttc gggcgccgtg atggatggaa
     2461 actgaatttg cccgataatc agacgtccga tggcccgggt atgaaacccg ccatttcgac
     2521 gaatccattt cgccgcggcc gatgcagctg ccacaatcga ggtttcctca acagccatcg
     2581 gaatgggtac gtcacgttca tcgattaaaa aattggtggc aatgcccaga ggtaaagaaa
     2641 acaccccgac cacgttttcg atcatgtgtt
//
LOCUS       DAOMXO010000316         2666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_21941_length_2666_cov_4.655687, whole genome shotgun sequence.
ACCESSION   DAOMXO010000316 DAOMXO010000000
VERSION     DAOMXO010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21941_length_2666_cov_4.655687"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2556)
                     /locus_tag="PLH57_07210"
     CDS             complement(<1..2556)
                     /locus_tag="PLH57_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02440.1"
                     /translation="MRGMNRFSLYLLVGVVTHVFTVNQSFADDFVDAGSEYGTLCDQN
                     CRNEYFDRLKGQTDGAANKESLKGQNPNAGDRSRLGEYEDNTTCDCVYNMKQLFDHEG
                     KKVQVIRNVGTPNIQFSDESIDLVKNNPRPGFFTRHIERRIENIRKKSEEEAKAKAAS
                     SPTVEPAVCRGANVDQKICSNRKEEIAKLDSIYKDLEAKKKRGETLSPEDQEKMAMYQ
                     DRRRELAEETLYEGTEKSSVDNNGAVAKASDQTDVQPGSTPEEGEAPATINDGRTGSE
                     TANGTGTQIPGDNPVADWAADNHLTPVTDNNGTVYCPRESGSTSYNYDSKTVSRGDEK
                     CLNEQQIKDEIAKAKKKKEDAAKDTAKKEEYASNQPKRKGWKGSDDSCGPTVNAMLVK
                     EAIALKGGNPSRDDLIHFMNTDSNFLNQREKIESKYSCDASITVLDVAEKIDQGMKVA
                     ATVTTGVVTGMAQYEVQKASGDPNQNVHRVANDQMANSMVKTGMMQTGMGAVQFIFKG
                     IAGLDINREQNTALKDLNKYNTDPNEVAKTGNQQTEADLTNAGATMAVLDRKAAAGPE
                     AAEEYNAEGEGYVQSSRNVHREVKDRAAQTSATGFIEGALNLAQGILSWKLGSDMKCD
                     EDNTSPACLGPMASPPPPLVGGSPEQVEASGSVPVPMPGVSGGDASPVIAEGVENLEE
                     EAFDDRFPLPGGGGGGGGGNNLLQRPDPHKANFVAGTGSGTGGSVGGGGGGGGGSAPR
                     GNEKGGVEKTLSASGKKFGDSLAAGGAGFGSSGGAAKRSDGTNMDMGGLFDKLGNLLP
                     NNRKEKKEDSSILDFGTRGIASAGSGTEVDDGTILGRNSNLFLRVSNTTMTYYK"
BASE COUNT          508 a          703 c          634 g          821 t
ORIGIN      
        1 cttgtaatac gtcatggtgg tgttgctgac gcgcaagaag aggttggagt tacggcctaa
       61 aatagtgccg tcgtcgactt cagtgcccga gccggccgag gcgatgccgc gggtaccgaa
      121 atccaagatg ctggagtctt cttttttctc tttgcgattg tttggcaata ggttgccaag
      181 cttgtcgaac aatccgccca tgtccatgtt cgtgccgtca gagcgtttgg cagcgccacc
      241 gctcgaccca aaccccgcgc caccggctgc cagcgagtcg ccgaattttt tacccgaagc
      301 actcaaggtt ttttcaacgc cgcctttttc gttgccgcgt ggagcggagc cgccgccgcc
      361 accaccgccg ccgccgactg agccgccagt accggaaccg gtgccggcta cgaagttggc
      421 tttatgagga tcgggccgtt gtaaaaggtt gttgccaccg ccgccaccac cgccgccgcc
      481 aggtagtgga aaacgatcgt cgaaggcttc ttcttcgaga ttctcaacgc cttcagcgat
      541 caccggcgac gcatcgccac cgcttacgcc cggcattggc accggcacgc tgccgctcgc
      601 ttcgacttgt tcaggagacc cgcccaccaa gggtggggga gggctcgcca tggggcctaa
      661 acaggctggg ctggtgttgt cttcgtcaca cttcatgtcg ctgcctaatt tccagcttaa
      721 aatgccttga gctaagttca aggcaccctc gataaaaccg gttgcactag tttgtgcggc
      781 acgatcctta acttcgcggt gaacattgcg ggaactttgc acgtagcctt cgccttcggc
      841 gttgtattct tcagccgcct cggggccggc ggcggctttt ctgtctaata ctgccatcgt
      901 cgctccagca ttggtgagat cggcttcagt ttgttgattg ccagttttcg ctacttcgtt
      961 tggatcggtg ttgtatttat tcaggtcttt tagggctgta ttttgctcgc gattgatatc
     1021 tagtccggca atacctttaa atataaattg aacggcgccc attccagttt gcatcattcc
     1081 agtttttacc atggagtttg ccatttggtc gttggcgact cggtgcacgt tctggttcgg
     1141 gtcgcccgaa gctttttgca cttcatattg agccatgccc gtcaccaccc cagtagtaac
     1201 ggtcgcggct accttcattc cttggtctat tttctcagca acatcgagca cagtaataga
     1261 ggcgtcacaa ctgtatttgc tttcgatttt ttcacgctga tttaaaaagt tcgaatcggt
     1321 attcataaaa tgaatcaaat cgtctcgcga agggtttccg ccttttagag caatggcttc
     1381 tttcaccaac atggcgttca cggttggtcc acaggagtcg tcggaacctt tccagccttt
     1441 tctcttgggt tggttgcttg cgtattcttc tttttttgct gtgtctttag cggcgtcttc
     1501 ttttttcttt ttcgccttcg cgatttcgtc ttttatttgt tgttcattta aacacttttc
     1561 atcgccccta ctgactgtct tgctgtcgta attgtagcta gtacttccag attcacgtgg
     1621 gcagtacacc gtgccattat tgtcggtgac cggagtcaga tgattatcgg ctgcccagtc
     1681 ggcgacgggg ttgtcgcctg gtatctgtgt gcctgtcccg ttcgctgttt cggaaccggt
     1741 tctaccatcg ttaattgttg cgggagcttc accttcttcg ggagtgcttc ccggctgaac
     1801 atcagtttgg tcggaggcct tggctacggc gccattatta tcgacactgc ttttttcagt
     1861 cccctcgtag agagtttctt ctgcgagctc cctacggcga tcctggtaca ttgccatttt
     1921 ttcctgatct tccggagaca gggtttctcc tcttttcttt ttcgcctcta ggtccttata
     1981 aattgaatcg agcttggcta tctcttcttt tcgattgctg caaatttttt ggtcaacgtt
     2041 tgcgccacga cagaccgccg gttcgacagt gggagagcta gccgctttcg ctttcgcttc
     2101 ttcttctgat ttttttcgaa tgttctcgat ccgtctttcg atatgtcgtg tgaagaagcc
     2161 aggacgcggg ttgttcttta ctaagtcgat cgattcgtcg ctaaactgaa tatttggtgt
     2221 gccgacattg cgaatcactt gaactttttt gccctcatga tcgaagagct gcttcatgtt
     2281 gtaaacgcaa tcacaagtcg tgttgtcttc gtactctcct aggcgacttc ggtcgcccgc
     2341 attggggttt tggcccttta aactttcctt attggcagca ccatcggttt gtccctttaa
     2401 ccgatcgaaa tattcatttc ggcagttttg gtcgcataaa gttccgtatt ccgagcctgc
     2461 atctacaaaa tcatcggcga aactttggtt gacggtaaaa acgtgagtta ccaccccgac
     2521 cagcaaatat agcgaaaatc gattcattcc ccgcattgca aaatccccca cactccttat
     2581 tttacggctt ttttggtcaa gtaatcaaga aagttgatta aaattatatg ttttatataa
     2641 aatatattaa aatcaaatat ttacgt
//
LOCUS       DAOMXO010000317         2659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22002_length_2659_cov_3.443932, whole genome shotgun sequence.
ACCESSION   DAOMXO010000317 DAOMXO010000000
VERSION     DAOMXO010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22002_length_2659_cov_3.443932"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..612
                     /locus_tag="PLH57_07215"
     CDS             127..612
                     /locus_tag="PLH57_07215"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRK02441.1"
                     /translation="MNTLLKRLAHFHTFGKANATAIAATVVDFGTLALLVEITHLHYV
                     LATAVAACLGAITNFLLNRTWAFSDWGNHARKKRRTAGLSKQLKRYALVSIMSALFNT
                     AGVFFFAEYAGFKYFTAKVLASILVAWLWNYPMHRRFVWPKEPRAVTPSTQGLYENTY
                     I"
     gene            593..1891
                     /locus_tag="PLH57_07220"
     CDS             593..1891
                     /locus_tag="PLH57_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRK02442.1"
                     /translation="MKTPTSKHLRETENRHLTPGLQNFAILADRAMKKGRGSKLWDVE
                     GREYIDLIGGIGVNALGHCHPKYVARLKQQLDELTIGSFTTEVRVELLELLAKVLPKH
                     LSRVQLYSGGSEAVEAALRLAKSHTKKWEFLSFWGAFHGKTMGTMALMGSTAKQGLGP
                     FPPGNISAPFANCYRCPFKLKYPSCGMLCAEFTRDTIKQQSSGALAAIIVEPMQGTAG
                     NIPPPKEWLSAIASIAKEFGALLICDEMITGFGRTGTMFASPEVGVEADIMTLGKGVA
                     SGFPLSMLACRAEIADAKPFAIPSGSSSSYGGNPLAAAAALASIQTIVDEKLVENSRA
                     QGEIFLARLQKWKSQFDFIGDVRGKGLFLAMELVKDHTTKTPIAKEKTESLFKAGVEK
                     GVLAMTYAASVRFQPALSINAEELNRGLDLLEETLREWQI"
     gene            1875..>2659
                     /locus_tag="PLH57_07225"
     CDS             1875..>2659
                     /locus_tag="PLH57_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02443.1"
                     /translation="MNGRSSAYRVGLIPPLVDSKKTILVIIGLWFALHAWLATGRSLW
                     MDELSRIEHVELGSLWLALKGVFSEPSPFTPGEIVFLYGVKKVFSNFLPLELWLRSFA
                     LLQGLLALWLSFKSRTPYLWFFTLFSASLTSHSTQARPYSALFLAGTFLYLTMFERTV
                     FTRSELWLMRVQTFFTHVYGLLFLLVAQLFRKDRLWAGITFVYSVAFTATYMKFHSGE
                     FSGWSSNLLLPPLGEFVRQTAGVLGNPHKASFLFLPLFLSWRF"
BASE COUNT          609 a          707 c          642 g          701 t
ORIGIN      
        1 ccccatgcac tacctattgt tgttcgcggt tgctctagta gcagtgtttg caaatttaac
       61 ggcaattgtg cgcttcgtag cgctctaccg ggcgttgcgg cgcagggatc gcactcgtcc
      121 tcgctcatga acactttgct caaacggctg gcgcatttcc acaccttcgg taaagccaac
      181 gccactgcca ttgccgccac ggtcgtggat tttggcaccc tcgccctgct agtggaaatt
      241 acccacctac actacgtgct agctaccgcg gtggcagcat gtttaggagc gattactaat
      301 tttttactca accgaacctg ggcgttttcc gactggggca accatgcccg caaaaagcgc
      361 cgcaccgctg ggctttcgaa acaattaaag cgctacgcat tggtttcgat aatgtcggcc
      421 ttgttcaata ctgcaggggt ctttttcttt gcagagtacg cggggttcaa gtattttacg
      481 gccaaagtct tggcttcgat tttagttgct tggttgtgga actacccaat gcatcgccgc
      541 ttcgtgtggc ccaaagagcc gcgagcggtc accccaagca cgcagggatt atatgaaaac
      601 acctacatct aaacatttgc gcgaaactga aaaccgccac ctaacgcccg gactgcaaaa
      661 cttcgcaatc ctcgccgatc gcgcaatgaa aaagggccgg ggctccaaac tatgggacgt
      721 cgagggtcgc gaatacatcg acctcattgg gggaattggc gtgaacgctc tgggtcactg
      781 ccaccccaag tatgtcgccc gcttgaaaca acaactcgac gagctgacca ttggtagttt
      841 tacaactgaa gttcgggtcg agcttttgga attgctcgcc aaagttttgc ctaaacacct
      901 ttctcgagtg cagttgtatt caggcgggag tgaagcggtc gaagcggcgc tccgcctcgc
      961 aaagtctcac accaaaaaat gggaatttct aagtttttgg ggggcctttc acggaaaaac
     1021 gatgggaacc atggcgctca tgggttcgac cgccaaacaa ggactcggac cgttcccccc
     1081 tggcaatatt tctgctcctt ttgccaactg ctaccgctgc cccttcaaac tcaaataccc
     1141 ttcgtgcggc atgctgtgtg cagagttcac tcgcgacacc ataaaacagc aaagctccgg
     1201 tgccctcgca gcaataatcg ttgaacccat gcagggcacg gccggcaaca ttccacctcc
     1261 gaaggaatgg ctgtccgcta tcgcctcaat cgctaaagag tttggcgcgc ttttgatttg
     1321 cgatgaaatg atcactggat ttggtcgtac cggaaccatg tttgcatcac cggaagtggg
     1381 cgtcgaagcc gacatcatga ctctaggaaa aggggttgct agtgggtttc cgctctctat
     1441 gctcgcttgc cgcgccgaaa tagcagacgc aaaaccattt gccattcctt cgggaagttc
     1501 ctcaagttac ggcggcaacc cgcttgctgc ggcagctgcg ctggcaagta ttcaaacaat
     1561 tgtagacgaa aaactggtcg agaactcgcg ggcacagggt gagatttttc tagctaggct
     1621 tcaaaagtgg aaatcgcagt tcgactttat aggcgacgtg cgaggtaagg gtttgtttct
     1681 tgctatggaa cttgtcaaag atcacacaac aaaaacacca attgccaaag agaaaactga
     1741 atcgcttttt aaggctggtg tcgaaaaagg cgtactagca atgacctacg ccgcttcggt
     1801 tcgatttcaa ccagcacttt caattaacgc ggaagaactc aaccgcggcc tagatttgct
     1861 agaggaaaca cttcgtgaat ggcagatcta gcgcctatcg tgtcggatta attccaccgt
     1921 tggtcgattc aaaaaaaaca atcttggtca ttataggcct ttggttcgca ctacacgctt
     1981 ggcttgccac cggtcgttca ctctggatgg atgaactctc ccgcattgaa cacgtagagc
     2041 tcggcagctt atggctcgca ctcaaaggcg tgttttcgga gcccagccct tttactcccg
     2101 gcgaaattgt ttttctctac ggcgtcaaaa aagttttttc aaactttctt cctttagaac
     2161 tctggctcag gagttttgcg ttactccagg gattgctcgc actctggctt tcgtttaaat
     2221 cacgaacacc ttacctgtgg ttcttcacac tgttttcagc ttcgctcaca agccactcca
     2281 ctcaagcccg gccctacagc gcgctatttc ttgctggtac gtttttgtat ttaaccatgt
     2341 ttgaacgtac ggtttttacc cgtagtgagt tgtggttaat gcgggtgcag acgtttttta
     2401 ctcacgtcta cggacttcta tttttgctcg ttgcgcagct atttcgaaaa gatcgtctct
     2461 gggccggaat cacttttgtt tatagtgtgg ccttcaccgc gacctacatg aagtttcaca
     2521 gcggagaatt ttcgggatgg tctagtaact tattgctccc accacttggg gaattcgttc
     2581 gacaaacagc cggcgtattg gggaaccctc acaaagcttc gtttttgttt cttccgctgt
     2641 ttctctcttg gcgcttctt
//
LOCUS       DAOMXO010000318         2655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22051_length_2655_cov_4.055769, whole genome shotgun sequence.
ACCESSION   DAOMXO010000318 DAOMXO010000000
VERSION     DAOMXO010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22051_length_2655_cov_4.055769"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PLH57_07230"
     CDS             complement(<1..1122)
                     /locus_tag="PLH57_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02444.1"
                     /translation="MAKSAAKSKKLGQENSVAPKRHLESVPSANYNRFWESSLFSDVY
                     LQNDVPVKYKELWDSYAEPFDNFCAQFRYFCEELKHDDFNSWTERETINKFIKPVLRM
                     LGYADKCSLNQEPWREDDAYTFKEHGESKTYKPDLVIVNDPRELKYIGNKEGQKKLDE
                     ARASVIIPIEAKYWNRIEDREVKSSDDSKRADKKDQSDSTRALDFDEQCLKYMEVLDK
                     DYGILTDGKTWRLYNRELSSDNYHRNFQFNLGHLIKHVNAGLEANQRDYDLFIENAKY
                     FFHIFSKRAVYSEDGGRRFLDDLLEYSKTYVTRVEDDLKTRFVRAMSHACNGFLRSAE
                     DAKESIELSKIRNVAESHLFNILFIRHCESRGILPLKHSD"
     gene            complement(1129..1365)
                     /locus_tag="PLH57_07235"
     CDS             complement(1129..1365)
                     /locus_tag="PLH57_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRK02445.1"
                     /translation="MSQKKNWHLGTVHWFDEKSGEGLIKGADGSLYFVHHSAIESVAS
                     AKSAKRLALRDKQKVKFQLLEDMTFTQVSRVKEI"
     gene            2227..2629
                     /gene="prs"
                     /locus_tag="PLH57_07240"
                     /pseudo
     CDS             2227..2629
                     /gene="prs"
                     /locus_tag="PLH57_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637112.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
BASE COUNT          695 a          655 c          612 g          693 t
ORIGIN      
        1 atccgaatgt ttgagaggta aaatacctcg cgactcacaa tgccgaatga aaaggatatt
       61 aaacaaatga ctttcagcga catttcgaat ttttgatagc tcaatcgatt ccttcgcatc
      121 ttctgctgat ctcagaaatc cattgcaagc gtgcgacatt gccctgacaa acctagtttt
      181 aagatcatcc tcgacccggg ttacataagt tttgctatat tcaagcaagt catcaagaaa
      241 gcggcgtccg ccatcttcgg aatagacagc acgttttgaa aaaatgtgaa aaaaatactt
      301 cgcattttct ataaatagat catagtcgcg ctgatttgct tctaaacctg catttacgtg
      361 tttaataagg tgacccaaat taaactgaaa atttcggtgg tagttatcgg aggaaagctc
      421 gcgattgtag agtcgccatg tttttccgtc ggtaaggatt ccgtaatcct tatccaaaac
      481 ttccatatac tttagacact gctcgtcgaa atctaacgcg cgggtggaat cactttgatc
      541 ttttttgtcg gcgcgctttg agtcgtcgga actcttgacc tcgcgatcct ctattctgtt
      601 ccaatatttt gcttcgattg gaataattac cgaagcacga gcctcgtcaa gcttcttttg
      661 cccctcttta ttgcctatat actttagctc acgaggatcg tttacgatta caagatcagg
      721 tttataggtt tttgactctc cgtgttcctt aaacgtgtac gcatcgtctt cacgccatgg
      781 ttcttgattc agtgagcatt tgtcggcgta gcccaacatt ctcagcactg gcttgataaa
      841 tttattgata gtttcgcgtt cggtccaaga attgaaatcg tcgtgcttta attcttcgca
      901 gaaatagcga aattgtgcac aaaaattatc aaacggttca gcatacgaat cccagagttc
      961 cttgtatttt acagggacat cgttctgaag atagacatca ctgaaaagcg agctctccca
     1021 aaagcgatta taatttgcac ttggaacgga ttccaaatgg cgtttgggtg cgacagaatt
     1081 ttcctgtcct aactttttag atttagctgc tgactttgcc atcggagatt agatctcctt
     1141 aacgcgtgac acttgtgtaa atgtcatatc ttcaagaagc tgaaatttta ctttttgttt
     1201 atctcgaaga gccagacgct tggcagactt tgcagaagcc accgattcga ttgctgagtg
     1261 gtgtacaaaa tacaaacttc catctgcgcc tttaatcaag ccctcgcctg atttttcgtc
     1321 gaaccaatgt acagtaccca aatgccaatt tttcttttgc gacataactt cctccgttga
     1381 agtcacgtga gaatttatcc agcgatcccc tttgaaaaag gcgatgcgca attagcaacc
     1441 atgctaggac aatcaagtaa ctcttcggct acgccgctaa aaactgaaga agattaacgg
     1501 catggctcgt tttcacaggg ccaattccgt cataggcaaa acatgcctct gaatagggca
     1561 aacgaatcag taattcgggg cacttataca tcgagacact tcaattgatt caaggagtta
     1621 cgtcctgagt ccgatgagtg gacgatggcc atcaagccgc ggctcctttc aaaatcgcgt
     1681 ttcaaactag gctgcgaatg cccaacgaag ctctttttca cgggcaaacc ggagttcgga
     1741 aacaccaaac tcgacaacgc atttctagcg gcactcgccg agggcgggtt tcaagtcggt
     1801 gcgctagcga aggcgtatca cgagaacggc gtcgaagtgg aaggactgga tgtcgcaaaa
     1861 gctgtgcagc agactacgga acttctcact caggaaaaca ttatcctcta cgaacccgca
     1921 attcagtttg gcgattatct cgttcgggta gacgtacttg taaagaaggg taaccaggtt
     1981 gagcttatcg aagtcaaagc caagagcttc gacacttccg aagaaaaccc cttcttcaat
     2041 aaaacgctcc tcaaaaaggg tatctataag ctcagcggcg actgggagcc atatcttctc
     2101 gacgttgcgt ttcaaaaata cgtactgaca aaagcgcatc cagactggaa aatctcggcc
     2161 catttaatgc tggctgacaa gaattcacgc gcatctgtag atggcctgaa ccagcgcttc
     2221 tttctgaaaa agagcgatga tggccaggtc ggcgttgtcg aggcgccgcg cgcgcgccaa
     2281 cgaatcgcgc gtcatgaaca tcatcggtga cgtcgaggat cgcacctgcg tgatggtcga
     2341 cgacatggtc gataccgcgg gtacgctgtg ccgcgcggcc gaggcgctca aggatcacgg
     2401 tgcgcgcaag gtcgtcgcct attgcacgca cccggtgttg tccggtccgg ccatcgacaa
     2461 tctgagcaat tcgaccctcg acgaactggt cgtcaccgac accattccgc tggcggagga
     2521 ggcgcaggcc tgcagcaaga tccgccagct gtcggttgcc ggcctgatcg gcgagagcat
     2581 caaccgcatc atctccggac aatcgctgag tgcgatctgc gtggattgag cacgctcggc
     2641 gcgcgtggcg cgcca
//
LOCUS       DAOMXO010000319         2652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22088_length_2652_cov_4.195610, whole genome shotgun sequence.
ACCESSION   DAOMXO010000319 DAOMXO010000000
VERSION     DAOMXO010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22088_length_2652_cov_4.195610"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1996)
                     /locus_tag="PLH57_07245"
     CDS             complement(<1..1996)
                     /locus_tag="PLH57_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRK02446.1"
                     /translation="MRKHKGYDWTFLADLGKSVSKTKSATSKDVERPFSRNIKAAKNN
                     AIYGAHTYHTKVPPEGIIPYIEYYTNPGDVILDPFSGSGMTGVAANLLAATSKDNIRR
                     VLLNDLGPAACHISYNHTHFVDPVTLRSIFESIAKKLSHEVDVAYRCWHVTKGGKAMR
                     GISLKQLESAAQVNLATTPSERIQFRYMGVDVELLRAEVSATIWSHAHRCPAGDTKQP
                     CGNRLILWDIAFDSKSGHMAKIFSCPNCKTRLTRADLEDRPEYVPVHMEYEYFCSATG
                     RTKRVKRATELFDRELIEKISKRTINAWYPNYDVAPHREMMTMGPAKLGIRKISDFYF
                     RRNLAVCARLWECINEVTDLRVRRALAFACTNTFWHATKMRRFNARGGARPLTGTLYI
                     PQISVECNVMRVLAKKITALEKYYSLFKTGTAPVSISNVSATMLNIPTNSIDYIFTDP
                     PFGGNIYYSDCSIIWEAWLERFTDESKEIHFNRSRKPADGGKTRDEYKVLIRNAFKEI
                     HRVLKPGKWASIMFNNSDNDVLEVFRGAAIDAGFLISDVQFFDKAQKSFKGYLGRNGK
                     QDVTNCDIVINVRKAPNHQTSTSSRRSTAVPLQKVTNEWVVLNVREYLKALPKRIQEQ
                     PKLFNNEHRTTPFLHAHLMRKIFADKKSFEGFTFEYLKALL"
BASE COUNT          715 a          583 c          586 g          768 t
ORIGIN      
        1 gcagaagggc cttcaaatac tcaaaagtaa aaccttcaaa ggacttctta tctgcaaaga
       61 tcttgcgcat aagatgggcg tgcagaaatg gggtcgttcg atgctcatta ttgaacaatt
      121 taggctgctc ctggattctc ttcggaagag ccttcagata ctcgcgaaca tttaaaacaa
      181 cccattcgtt cgtaactttt tgaagaggta cagcagtgct tcgacgagaa ctcgtgctgg
      241 tttggtgatt tggggcttta cgaacgttga ttacgatatc gcaattggta acatcttgct
      301 ttccgttccg acccaggtag cctttaaagc ttttttgtgc tttatcgaag aactgtacgt
      361 ctgaaatcaa aaaccctgca tcaattgcag caccacgaaa tacctcaaga acatcattgt
      421 ccgagttatt aaacataatt gatgcccatt ttccgggctt aaggactcga tgaatttctt
      481 tgaaggcatt gcgaattagc actttgtact catcccgggt tttgccacca tctgcaggtt
      541 ttcgacttct gttgaaatga atttcttttg attcatcggt gaatcgttcg agccaggctt
      601 cccaaataat tgaacagtcc gagtagtaga tatttccacc aaaaggtggg tctgtgaata
      661 tatagtctat gctattagtg ggaatattca acatggtggc acttacattc gaaatactta
      721 ctggcgcagt accggtctta aatagagaat agtatttttc tagagcggtt atttttttcg
      781 ccaatactcg cattacgttg cattctactg agatttgcgg aatgtacagc gtaccggtaa
      841 gagggcgagc accgcctcgc gcgttgaatc tgcgcatctt ggtcgcgtgc caaaaggtat
      901 tggtacaggc aaatgctaac gctcgtcgaa cacgaagatc agtaacttca tttatgcact
      961 cccataaacg tgcgcagacc gccaaattac gtcgaaaata gaagtctgag atttttctaa
     1021 tgccaagctt tgctggcccc atagtcatca tttctctatg tggagcaaca tcgtaatttg
     1081 gataccaagc attgattgtg cgctttgaaa ttttttcgat taattcgcga tcaaataact
     1141 cagtcgcgcg ctttaccctt ttggtacgcc cggtcgccga acaaaagtac tcatactcca
     1201 tgtgcacagg aacatattcc ggtcgatctt ctaaatccgc tcgggtaagg cgcgttttac
     1261 aatttggaca gctaaagatt ttagccatgt ggccagactt cgagtcaaat gcgatgtccc
     1321 acaagatcaa cctatttccg cacggctgtt tagtatcccc cgcagggcat cggtgggcat
     1381 gtgaccaaat ggtagctgag acttctgcac gcaacagttc gacgtctaca cccatatacc
     1441 gaaattggat gcgctcactg ggagtagtcg caaggtttac ttgagctgca gattccagct
     1501 gctttaagga aatacctcgc atcgccttgc cacccttagt aacgtgccag catctataag
     1561 ctacatcaac ctcatgggac agcttcttgg caatgctttc gaaaatgcta cgtaaagtta
     1621 cgggatcgac gaaatgtgta tgattatatg aaatatgaca agctgcgggt ccgaggtcat
     1681 ttaagagaac tcttctgata ttgtcctttg aagttgccgc aagcaagttt gcggcaaccc
     1741 cagtcatgcc gctgccggaa aaaggatcaa gaattacatc tccagggtta gtgtaatact
     1801 cgatatatgg aatgatccct tccggaggaa cttttgtatg ataagtgtga gctccataaa
     1861 tcgcgttgtt tttcgcagcc ttgatatttc ggctaaatgg gcgctccaca tcctttgaag
     1921 tagcggactt ggttttagaa acagatttgc cgagatctgc aagaaaagtc cagtcgtatc
     1981 ccttgtgttt gcgcattcgt ccttgaacgc tcccgtcctt tcagtttgtg gcttaaagga
     2041 gcggccggca actataacag ctacgttttc ttggaccaaa gggcaaaaat ttccacctca
     2101 gtgttgattc aagcaattag agatttctgg gctgattggt agaattcgaa gagattcctt
     2161 ttcaagtata caaaaatgac cgttaccaaa tcgctaaaaa aacatttgca aggttagtaa
     2221 acggcaattt tccttatcag gagaggaatt gtaacgatcc ccctcccgaa ccatctttcg
     2281 gtggcaggcc taatgggttt tctgtttgca attattgcca aattttcctc aagattatcg
     2341 aaagaacggt tttagattcg gaccgccttt cacagatttt ataacctctt ttcgagccca
     2401 agattgaagt ctatccaatt ggcccgcaga agccgctaaa atgcataaac cgagtagtgc
     2461 agaaaatagt ttagaaatat cagccatgcg cattagtttt tcccttcatg aagagtttaa
     2521 atcgcattct ttgaaagttg atagccacac atccattttt tgggggacac gtgggggaca
     2581 ggagaggcaa aatcggttct aatgggtgca tttctgttca gtggcggtga actagtaagc
     2641 tcgtgtgatt at
//
LOCUS       DAOMXO010000320         2651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22098_length_2651_cov_4.808937, whole genome shotgun sequence.
ACCESSION   DAOMXO010000320 DAOMXO010000000
VERSION     DAOMXO010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22098_length_2651_cov_4.808937"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLH57_07250"
     CDS             <1..458
                     /locus_tag="PLH57_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008549566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK02447.1"
                     /translation="LNGFTKALQISAYSLVELARRVEPFMERAGGGRILTLSYYGAEK
                     VVPNYNVMGVAKAALEASVRYLANDVGSKKITVNAISAGPVKTLAAAGIKNFRSILDT
                     SAARVPLRENIEAEDVGHLAAFLCGPGGAHITGSTMYVDSGAHIMGMGA"
     gene            873..1184
                     /locus_tag="PLH57_07255"
     CDS             873..1184
                     /locus_tag="PLH57_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02448.1"
                     /translation="MMVEDDAFGYKTVAGPQFSVALHRALPNKAAGLMFFRFAPLFDH
                     TGQFAIANRSMGTGIAYTTPTTEIGSALSINLEFEFISIPLPTTIQASSSATLALGLT
                     L"
     gene            complement(1188..2498)
                     /locus_tag="PLH57_07260"
     CDS             complement(1188..2498)
                     /locus_tag="PLH57_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02449.1"
                     /translation="MFFYIVVAVGGFLWSLGTFTKNTSENDQPTPLEQSIRVSWKPRK
                     SKSSVPVTVVRSSWEWVTSESSIDGDSPTSVRPVLRFFIELDLPGIAPEEIQKLNTSR
                     LDLQIVQSELLETGRALLGVDLTSVKSETTVFLDRKNLGKLAVEVSPAPQKVRVWRAC
                     PSYEFGYRRPARERPFLYVGVHCREEKSGLWIELIPSEEKGAKPQLIELQHATRQIIN
                     VADKIPSHPLVIGLRKKLSPRYGIEVGWDTVFTNYKEGTRHEITMASLATRFGYTRDL
                     DPNWSLRSMLSATIFPIAQNVQNLDIHFVNGTLAAVYQRPLDEQWSLGGLVGLYYVSM
                     FTRRATFGYRHQLGPQLAPHLRVQIGEDDDLTFGGRVAAVADRGFKLMEISNNELGGS
                     FGWRRLLSDKTRVGISLAYTRLELKIKTTQLKSNHFAIQTAFEF"
BASE COUNT          655 a          701 c          599 g          696 t
ORIGIN      
        1 gtttgaacgg ctttactaaa gcgcttcaaa tcagcgccta ctcgttggtc gaactcgcac
       61 gaagagttga accctttatg gagcgcgccg gaggcggtcg cattctcacg ctttcttatt
      121 acggtgctga aaaagtagtt cccaattata acgtcatggg cgttgcgaaa gcggctctag
      181 aagcaagtgt tcgatactta gctaacgacg ttggttctaa gaaaatcacc gtcaacgcca
      241 tttcggcggg accagttaaa acattggctg ctgccggtat aaaaaacttt cgctcaattc
      301 tagacacatc agccgccagg gttccgctgc gcgaaaacat agaggccgaa gacgttggtc
      361 atctggcggc atttctttgc ggcccaggtg gcgcgcacat cacgggttcg acgatgtatg
      421 ttgatagcgg tgcccacatc atgggaatgg gtgcttaagt cctaaccacg tcagatgcaa
      481 atgtttgtat ggctattttt cgttttgatt cacacagcga ccgctgccga cgctcctcag
      541 aagcgccaac tcgaatttgt caccgttagg ccggccgaaa aagcggtcta ctggaccccc
      601 tccatcggat taactagttt tcgattccgt caaacgtcgc gcaccgatta taacgcgctt
      661 tttttccaaa caaaattatt gggtcgagtt cctatttccc gttcttggga ttttggtggc
      721 gcgctcgcac taaacttgct gccagtctcc caacaacctt catcgcattc tacccgtgtt
      781 gggagccttg ccttacggtt cggctactcc cctgccactc tggtccctcg ccgttgcgat
      841 tggccctact ggctggccct tacgtagttc atatgatggt tgaagacgac gccttcggat
      901 acaaaaccgt ggcgggccca caattctcgg tggctctgca ccgagccctt cccaataaag
      961 cggcaggcct aatgttcttt cgcttcgctc cactttttga ccacaccggg cagtttgcaa
     1021 ttgccaaccg atcgatgggc actggaatcg cctacaccac cccgaccact gaaatcggca
     1081 gtgctttatc gataaatcta gagttcgaat ttatatcgat tcctctacca actacaattc
     1141 aggcttcatc gtccgctaca ctcgcacttg gacttaccct ttagtattta aaactcaaat
     1201 gccgtttgaa tggcaaagtg gttcgacttc aactgagtgg tcttaatttt caattccagg
     1261 cgggtgtagg ccaaacttat tcccacccga gttttgtctg aaagcaaacg cctccaacca
     1321 aatgaacctc cgagttcgtt gttactaatt tccatgagtt taaaaccacg atccgccact
     1381 gctgcgactc gtcccccgaa cgtaagatcg tcatcttcgc cgatctgaac tcttaaatgg
     1441 ggggccaact gcgggcctag ttgatgacga tatccaaacg tagctctgcg agtgaacatg
     1501 gaaacataat acaatccaac caatccgcct agactccatt gttcatcgag aggacgctga
     1561 tacactgcgg ccaacgtgcc gtttacaaag tggatgtcga gattctgcac attttgcgcg
     1621 atcggaaaaa tagtggccga tagcatgctt cgtaaactcc aattcggatc taaatcacgc
     1681 gtgtaaccaa accgcgtggc aagcgacgcc atggtgattt catgacgggt tccttcttta
     1741 tagttggtga aaacggtatc ccaacctact tcgatgccat agcgagggct aagttttttt
     1801 cgcaacccga tcacgagcgg atgactagga attttatctg ctacattaat tatttgtcgc
     1861 gtggcatgtt gaagctctat caactgtggt tttgcgcctt tttcttcgct gggtataagt
     1921 tcgatccaaa gccctgattt ttcttcccga caatggaccc ccacgtaaag aaacggccgt
     1981 tcgcgagctg gccggcgata cccaaactcg taagatggac acgcgcgcca gactctaact
     2041 ttctgaggtg cggggctcac ctccactgcc aacttaccca gatttttgcg atccaagaaa
     2101 accgtagttt cacttttcac tgaagtaaga tccacaccta gcaatgcccg gcccgtttcg
     2161 agtaattcgc tttgaacgat ttgaagatcg aggcgactgg tatttagctt ttgaatttcc
     2221 tccggagcga taccaggtag atcgagttca ataaaaaatc gcaacactgg gcgtaccgag
     2281 gttggcgaat ccccgtcgat cgaggattca ctggtaaccc attcccagga actgcggacc
     2341 acggtcactg gaacgctcga cttactcttt ctgggtttcc acgaaaccct gatcgactgc
     2401 tcaagtggcg tcggttgatc gttttcagaa gtattttttg tgaaggtacc gaggctccat
     2461 agaaagccac ccaccgccac gacgatgtag aaaaacacac gtttacacgt cagaaaaatt
     2521 tcctctattt accagtgtct gagacttctt aaaagcgcgc aaccagttga aagcaggagt
     2581 ttttttccgt tatttgggac taagaaaatt aggcagaatt ctcctacgca attgtttaaa
     2641 aaataaaaaa a
//
LOCUS       DAOMXO010000321         2644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22169_length_2644_cov_5.222480, whole genome shotgun sequence.
ACCESSION   DAOMXO010000321 DAOMXO010000000
VERSION     DAOMXO010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22169_length_2644_cov_5.222480"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /gene="glmM"
                     /locus_tag="PLH57_07265"
     CDS             <1..990
                     /gene="glmM"
                     /locus_tag="PLH57_07265"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRK02450.1"
                     /translation="AQEAEIERLLHSKELEKCRPTGDSVGKAFRIDDALGRYIVQLKN
                     VFPKDLDLTGFRIVLDCANGAAYKAAPAVFEELGAEVIRKGVSPTGLNINDKCGALYP
                     ENVAAAALEYRADIGIALDGDGDRVILADENGEIVDGDQIMAICALDMKSKNQLSLNT
                     IVATPMSNLGFEIGMQKAGIQVVRAQVGDRYVVEAMRQGGYGLGGEQSGHIVFLEHAT
                     TGDGVVGALKVLEVMRRSKKPLSELKRCVDLMPQVREDVKVARKEPIHNLKDVVAEIS
                     AAEKTLGQKGRVLVRYSGTESIARVMVEGEDLRQIQSLSKKIAVTIFKAIGQA"
     gene            991..2583
                     /locus_tag="PLH57_07270"
     CDS             991..2583
                     /locus_tag="PLH57_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRK02451.1"
                     /translation="MRVVSTEEMRDLEKVASDDLNLSSLMLMENAGIGAAEIISKILS
                     QRYPGVDPEVLVFCGRGKNGGDGLVVARQLLARGGRVRVFLMHKKEDHTGETAHNLSI
                     LERQKAKIVQVPTAAVVEEYFQSAASRPFVVVDALLGTGISRPIEGAYFDTVQSINRH
                     ADTLISLDVPSGTDANNGSLWGSSAQATHTITFGYPKIGLYQMPAASKRGRLYCVPLS
                     FPSTWGDQGDKRLLNESNTATLLQSRDRFGHKNSFGHTLFIGGSPGRLGAVVMASQAA
                     LKMGSGLVTVASWPESYPSLENKLSPEVMNFKISKESGEFYVPKPGLNAFSSIVAGPG
                     LGVSEEAAQLLQQLIKLYTGALVLDADALNILAEFKLQNLLLKRVQPTVLTPHPGEMA
                     RLLGVDKNVVVEQPIHCVRQAVEATTATVVLKGATSFVHSAEGITWINHYPNDGMATA
                     GSGDVLAGMIGGLMGQGMSAVDSAKCGVFLHSLSGKAAAERFGHRSMTAGSIIENIST
                     AFATLAEFQSPCSLSEFYVELV"
BASE COUNT          655 a          660 c          695 g          634 t
ORIGIN      
        1 gcgcaggaag ccgaaatcga acgccttctt cactcgaaag aacttgaaaa gtgccgccct
       61 accggagatt cggtaggcaa agcgtttcga atcgacgacg ctcttggcag atacattgtt
      121 cagcttaaaa atgtttttcc caaagacttg gacttaaccg gctttcgcat cgtactcgac
      181 tgtgccaatg gagcggccta taaggcagcc ccagctgttt tcgaagagct tggagccgaa
      241 gtcattcgca agggcgtttc tcccactggt ttgaacatca acgacaaatg tggagcgctc
      301 tatccagaga acgttgcagc cgctgcgctc gagtatcgag cggacatcgg tattgcactc
      361 gacggtgatg gcgaccgggt catattagct gacgaaaacg gcgaaatcgt tgatggcgac
      421 cagattatgg cgatttgcgc tctcgacatg aaaagtaaaa atcagctctc gctcaacact
      481 attgtcgcca cccccatgtc gaatctggga tttgaaatcg gcatgcagaa ggccggcatt
      541 caggtggtgc gggcccaagt cggtgatcga tacgtagttg aagcgatgcg ccagggcggt
      601 tacggtcttg gtggcgaaca gtccggacac atcgtgtttt tagaacacgc caccaccggg
      661 gacggtgtcg ttggggcact taaagtgttg gaagtaatgc gacgttcgaa aaaaccgctc
      721 tctgagctaa agcgatgtgt agatcttatg cctcaggtgc gagaagatgt gaaagttgcg
      781 cgtaaggaac caattcacaa tcttaaagac gttgtggcag aaatttctgc agccgaaaaa
      841 accctcgggc aaaaaggaag ggttcttgtg cgttactccg gcaccgaatc tattgcccgc
      901 gtaatggtcg aaggcgaaga tttgcgccaa attcaatcgt tgtccaaaaa aattgcggta
      961 actattttta aagctatagg acaagcataa atgcgagtcg tatctaccga agaaatgcgg
     1021 gacctcgaga aagttgcgag tgacgatcta aacctttcgt ctctcatgct gatggaaaac
     1081 gccggtatcg gtgccgccga aattattagc aaaatactat cgcagaggta ccccggagta
     1141 gatccagaag ttttggtctt ttgcggacga ggaaaaaatg ggggcgacgg tttggtagtc
     1201 gcccggcagt tgcttgctcg cggcgggcgc gtacgcgtgt ttcttatgca caagaaggaa
     1261 gatcatactg gcgagactgc tcacaatttg tcgattcttg agcggcaaaa agccaaaatc
     1321 gtgcaggttc ctaccgctgc agtggtcgaa gaatactttc aaagcgccgc ttcacgtcct
     1381 ttcgtagtcg tcgatgcatt gcttggaacg ggcatcagtc gccccatcga aggcgcttat
     1441 ttcgacaccg tacagtcgat caatcgccac gccgacactc taatttcgct agatgttccc
     1501 agtggaaccg acgctaacaa cggaagtctt tggggctcca gcgctcaagc cacccacacc
     1561 attacatttg gctatccgaa aatcggactt taccaaatgc cggctgcttc taaacgcggc
     1621 cgcttgtatt gcgtgccttt gtcatttccg tcgacttggg gtgatcaagg agacaagcgt
     1681 ttgcttaacg aaagcaacac agccacttta ctgcagtctc gcgatcgatt cggacacaaa
     1741 aatagctttg gtcacaccct ttttatcgga ggcagccccg gccgcctcgg tgccgttgtc
     1801 atggccagtc aagcagctct taaaatgggc agcggcttgg taacagtagc gagttggccc
     1861 gagtcctacc cgtcgcttga aaacaaactt tcgcccgaag tcatgaactt caaaatttca
     1921 aaagagagcg gcgagtttta tgttccaaaa cccggtctca acgcgttctc atctattgtt
     1981 gctggcccgg gactaggcgt atcggaagag gccgctcagt tgttacagca gctcatcaaa
     2041 ctttacactg gcgccctcgt actcgatgca gatgccttaa atattctcgc cgaattcaaa
     2101 cttcagaact tgttattaaa acgtgtgcag cccacggttc tcactcctca tcctggagaa
     2161 atggcccgac tcctcggagt cgacaaaaac gtggtcgtcg agcaaccaat tcactgtgtt
     2221 cgacaagctg tcgaagcaac aacggctaca gtggtgttga agggcgcaac gtctttcgta
     2281 cattccgccg aaggcatcac ctggattaac cattacccca acgatggaat ggcaactgct
     2341 ggcagcggcg atgttctggc tggaatgatc ggcggcctta tggggcaggg gatgagcgct
     2401 gtcgactctg ccaagtgcgg tgtgtttctt cacagtttgt ctggaaaagc ggctgctgag
     2461 cgattcgggc atcgctccat gaccgctggg agcattatcg aaaatatttc gactgccttt
     2521 gcgactctag cagaatttca aagcccatgt agtttgtcag agttttatgt cgaactggtc
     2581 tgattatttt gttggatcag tgtccgaggg tgagctcgta tcgttggccc agaagcttgc
     2641 ccac
//
LOCUS       DAOMXO010000322         2642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22194_length_2642_cov_4.411287, whole genome shotgun sequence.
ACCESSION   DAOMXO010000322 DAOMXO010000000
VERSION     DAOMXO010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22194_length_2642_cov_4.411287"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="PLH57_07275"
     CDS             <1..1761
                     /locus_tag="PLH57_07275"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRK02452.1"
                     /translation="IRINDAPTMYLPYAIIPVKRQRQTGFLFPKMQLVSPNGFTYIQP
                     FFWAISRSQDATISGGLYTRKGYKAEGQYRFMFTPRTYGELNSFYLYDRTFTRPYNNR
                     WAFQYKHNVRLPFKIDQKLHLLDTSDRDYPRLIGDIPGRGEPALVSEGVLSRSGRDLS
                     VHVKAKRIKNLLTTSINNWDKNTVQLVPGFSIATTDHRVSSSVPLHWGLKFQFDRFWR
                     QGPSFDAIYDYPVKTATPSNNFFLPGQTPLRQAQRYNLIPELYYTTKLADVIEVIPLA
                     QYRTFHYRFDENVVGPTSRGYLLLQNEVATVIERVYSGKMKHKIRPSLIYSRIPIVQQ
                     NVDHPFNKQIRTAGYQFDELDIVPITSDTQQYFVPLGNSIEYKVNNRFILKSDDPTPT
                     YRKIVEVTSGQTFNFIELQKDSKDERPFSRFYTLLSVDMLRLSGTGSFYYYPYVKRSS
                     LSVGAKYTFGKYLRRVMLFERSLGLRYRTERVISNDHGIGGDLNWSFNDYIGVLFSTD
                     YDFPVQKEGVSRTGRIRKISGGVTYQSPSQCYKMVLMAGWVSDQREISVNFTVPINLT
                     GDGFTDIGDSDSLQNAGGAK"
     gene            1765..2496
                     /locus_tag="PLH57_07280"
     CDS             1765..2496
                     /locus_tag="PLH57_07280"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRK02453.1"
                     /translation="MKRKQPYIAAIEELIRTLSRKNELAVKKVVGRLARSIDNGGLLY
                     VFGSGHSAILVEEVFHRAGGLVPVYPILTTVLTPHTTPKISGPLERLSGIAAILFKKA
                     APTSKDILFIASNSGVNAAAIEMALEAKKNKVWTVAFTSVEHSKAAATRHSSGKRLKD
                     IVDVVLDNCAPVGDATVDFGETKVGAVSTIANSILYHWILTDMCARWKRAGKKLPVYR
                     SANLPGGDEYNERQEKKVRKRIPLL"
     gene            2526..>2642
                     /locus_tag="PLH57_07285"
     CDS             2526..>2642
                     /locus_tag="PLH57_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRK02454.1"
                     /translation="MKILLSNDDSVHAWGLEVLQAHLKKIADVVVVAPLEEKS"
BASE COUNT          710 a          598 c          636 g          698 t
ORIGIN      
        1 attcggatca acgatgcacc aacgatgtat ttgccctacg caatcatccc cgtaaaacgc
       61 caacgtcaaa ctgggttttt gtttccgaaa atgcaacttg tatcgcccaa tgggtttacc
      121 tacattcaac cgtttttctg ggcgatttcc cgcagtcaag atgcgaccat atcgggtggt
      181 ctctatactc gaaaagggta caaagctgag ggacagtatc ggttcatgtt tactccgcgg
      241 acctacggtg agttaaattc tttttatctc tacgaccgta cttttacccg cccctacaac
      301 aatcgttggg catttcagta caaacacaac gtgcgacttc cttttaagat cgatcagaag
      361 cttcatttgc tcgacacttc cgatcgcgat tacccacgct tgatcggaga tattcccggt
      421 cggggcgaac cagcgctcgt gtccgaggga gtgttgtcgc ggtcggggcg cgacctgagc
      481 gtgcatgtaa aagccaagcg gattaaaaat ttattaacta ccagtattaa caattgggat
      541 aaaaatacag tccaactggt cccgggattt tcgattgcga ccaccgacca tcgcgtatca
      601 tcgagtgttc cactgcactg gggtcttaag ttccaattcg accgtttctg gcgacagggc
      661 ccgagtttcg atgcgatcta tgattatcca gttaagacgg cgactccatc gaataatttt
      721 tttcttccag gccaaactcc gctacgccaa gcccaacgct ataacctgat tccggagctg
      781 tattacacca ccaaactcgc cgatgtgatt gaagttattc ccctggcgca ataccgaacg
      841 tttcattacc gcttcgacga aaacgtcgta ggtccaacgt cccggggata tttgttgtta
      901 caaaacgaag tcgccacagt tattgagcgt gtttattccg gcaaaatgaa acacaagata
      961 aggccttcac tgatttactc ccgtattccg attgtgcaac aaaacgtgga tcaccctttt
     1021 aataaacaga ttcgcacggc gggctatcag ttcgacgagt tggatatcgt gccgatcacc
     1081 agtgatactc aacaatactt cgtgccactc ggaaactcga tcgaatataa agtgaataac
     1141 cgctttatct taaaatcgga cgatcctacg cccacctacc gcaaaattgt cgaagtaacc
     1201 tcggggcaaa cgtttaattt tattgagtta caaaaagaca gcaaagacga acgaccgttt
     1261 tcccgtttct atactctgct ttcggtagac atgttgcggc tcagcggtac agggtcattt
     1321 tactactatc cctacgtaaa gcgttcttcg ttgtcggttg gtgcaaaata tacttttgga
     1381 aagtatctcc ggcgagtgat gctttttgaa cgaagtcttg ggctgcgtta tcgtaccgag
     1441 cgagtgattt cgaacgacca cggaatcggt ggggatttga attggtcgtt taacgactat
     1501 atcggggtac tgtttagcac cgattacgac ttcccagttc aaaaagaagg ggtttcacga
     1561 accggacgaa ttaggaaaat ttccggaggc gtcacttatc aaagtccttc gcaatgttac
     1621 aaaatggttt taatggcggg ctgggtgtca gaccaacgag agattagcgt gaattttacg
     1681 gtaccgatta atttaacagg agacggattc acggatatcg gtgattcaga ttctttgcaa
     1741 aacgcaggtg gagcaaaata atatatgaaa cgaaaacaac cttacattgc cgcgatcgag
     1801 gagttgattc gtacgttgag tcgaaaaaat gaattggctg taaaaaaggt agtcgggcga
     1861 cttgctcggt ccattgataa tggcggctta ttgtatgtgt ttggaagtgg tcattctgcg
     1921 attttggtcg aagaagtttt tcatcgcgcg gggggactag tgccagtgta cccaattttg
     1981 accaccgtgt tgactcctca cacaacgcca aaaatatctg gtccccttga acgtttgagt
     2041 ggcattgcag ccattttatt caaaaaagct gctcctactt cgaaagacat tctctttatt
     2101 gcgagcaatt ccggggtgaa cgccgcggct attgaaatgg cactcgaggc gaagaaaaat
     2161 aaagtctgga ccgtggcgtt taccagtgtt gaacactcca aagcggccgc gactcgccac
     2221 tcaagtggca aacgattaaa agacatagtc gacgtggttt tggataattg tgcacccgtg
     2281 ggcgatgcga cggtcgattt tggtgaaaca aaagtgggcg cggttagtac aattgccaac
     2341 agcatacttt atcactggat attgaccgac atgtgcgcgc gttggaaacg ggctgggaag
     2401 aagttgccgg tctatcgaag tgcgaactta cctggcggtg atgagtacaa cgagcgtcaa
     2461 gaaaagaaag tgcgcaaacg gattccgctt ttgtgagttt gtgtgcttta aggtaaaata
     2521 aaattatgaa aattcttcta tcgaacgacg acagtgttca cgcctggggc ctcgaggtgt
     2581 tgcaggcgca tcttaaaaaa attgcggatg ttgtagtagt ggctccccta gaggaaaaaa
     2641 gt
//
LOCUS       DAOMXO010000323         2638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22263_length_2638_cov_4.903213, whole genome shotgun sequence.
ACCESSION   DAOMXO010000323 DAOMXO010000000
VERSION     DAOMXO010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22263_length_2638_cov_4.903213"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>664)
                     /locus_tag="PLH57_07290"
     CDS             complement(<1..>664)
                     /locus_tag="PLH57_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02455.1"
                     /translation="DGKTNMLRLTASGENTIGALESPSFSDQRTNAVIVRNGVIEIVL
                     TADAVTRGVEYFDYRTKNSGDVSIDYWFKNAEPTVQNSRQTPNKSGATASKKSNQANL
                     ARKKITSKKSIHTGVCSERLDLTKDGYTYWNIYHVSFPYSRYFNLDTADAAYEYPFSE
                     QDQKATKKHNARELAHYRLALKLNKESKLALLLRTIAFFEENFPKSELKKELDFLRAA
                     TFV"
     assembly_gap    665..674
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<675..884)
                     /locus_tag="PLH57_07295"
     CDS             complement(<675..884)
                     /locus_tag="PLH57_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02456.1"
                     /translation="MVQSEIKNPTTEKAMKSGLLTALVVLVLTTASAASMPALAGSLE
                     FKSFQTHSHLTIPAAYARKYSVELVG"
     gene            1173..1706
                     /locus_tag="PLH57_07300"
     CDS             1173..1706
                     /locus_tag="PLH57_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02457.1"
                     /translation="MNITRISLAVLLIALSATLSGCGLIFGGDQSVDTKSRDYSVLRL
                     DKLQRSKWRTIESRSTPDSSDVAYENPSTGSIISLNSICRGNEKSSLKELSRALMMGL
                     KKQSSIETREYSISGISALESTVDAETIDHKTVRIRTVVMRKDRCTYDVMYVSPRENF
                     HRELNVFNDFLKGFHVE"
     gene            1696..2493
                     /locus_tag="PLH57_07305"
     CDS             1696..2493
                     /locus_tag="PLH57_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK02458.1"
                     /translation="MLSKDFFRNIGAQFVGGLQFAGGVTALGFDCLKELFRPPFYIRL
                     TLEQMFLIGVKSFSLVTITALATGSVMALQFGYGLEKFGGKLYVPKVMALAFVREMGP
                     VFTSLLVAARIGSGITAEVGSMNVTQQIDAIRALGTNPIKKIVLPRLLATMITLPLLT
                     VMADFIGLFGGMLVTVKELGQTPEFFFTKAVEALTLSDFVMSLFKPFVFAIFIALTAC
                     YRGMRTLGGTRGVGDSTTWVVVTTSVFIMVSDFFLTKAFILFVPSPK"
BASE COUNT          641 a          668 c          599 g          720 t
ORIGIN      
        1 gtacgaacgt agctgcccgc aaaaaatcga gttccttttt cagttcactc ttcggaaaat
       61 tttcttcaaa aaatgcgatt gtacgcaata acagagccag ctttgattct ttattaagtt
      121 tcagagctaa ccggtagtgc gcaagttcgc gagcgttgtg cttttttgta gccttctggt
      181 cttgttccga aaacgggtac tcgtaagccg catccgctgt gtctaaatta aagtagcgac
      241 tataaggaaa ggaaacatgg taaatattcc agtacgtata gccatctttg gtaagatcaa
      301 gccgttccga acaaacccca gtgtgaatac ttttcttaga tgttattttt tttcgtgcta
      361 agttcgcttg attacttttc ttactcgccg tcgcgccact tttgttgggc gtctgccgcg
      421 aattctgaac ggtcggttcc gcgtttttga accagtaatc tattgatacg tcgccggaat
      481 ttttagtccg atagtcaaaa tattccacac ccctggtcac agcgtcagca gtaagaacga
      541 tttcaatgac gccattgcgc acgatcactg cattggtgcg ttgatccgaa aaagaaggtg
      601 actccaacgc cccaatagta ttttcgccgc tcgcggttaa gcgaagcatg ttggtttttc
      661 catcnnnnnn nnnnaccgac gagttctacc gagtacttcc gagcgtaggc ggcaggaatt
      721 gttaaatgag agtgggtctg aaaagacttg aactccaagg aaccagctaa cgccggcata
      781 gaagccgccg aagcggttgt caaaaccaac accactaggg cggtcaacag cccggatttc
      841 atagcttttt cagtggttgg atttttgatt tctgattgaa ccacgatttt gctctctcca
      901 tgagcagtgg gattttccgc gatcacatcc ttgatcgctg cctactaggt aagcagatac
      961 cgtgccaaaa tctgcctgtc gaaatcattg ctttttttgc cgcccggttc tttttttacc
     1021 tgaaaaaacg acacgattcc gacgaaactt cagtcattgt tttgacggga gccatctaag
     1081 catcggcatt aatggcacag tacttcagat tgacgccacc gccgccgcct cctaaactta
     1141 aagcttaaca acggtttgac ccagaaagac ccatgaacat cactcgtatt tcacttgcgg
     1201 tattattgat agctctcagc gccactctct cggggtgcgg tttaatcttc ggtggtgacc
     1261 aatcggtcga cacaaaaagt cgcgactaca gtgtcctgcg gctcgacaag ctccagcgca
     1321 gtaaatggcg aactatagaa tctcgtagca cgcccgactc aagcgacgtt gcttacgaga
     1381 acccgtcgac cggctcgatc atttctctta atagtatatg ccgaggcaac gagaaatctt
     1441 cgctaaagga actctcccga gcccttatga tgggccttaa gaagcaaagc tcgattgaaa
     1501 ctcgggaata ctcgatcagc ggaatttccg ctttagaatc tacggtggat gccgaaacaa
     1561 tagatcacaa aactgtgcgc attcgcaccg tggtaatgcg aaaagaccgc tgtacctacg
     1621 acgtaatgta cgtgtccccc cgcgaaaatt ttcatcgtga attgaatgtc ttcaatgact
     1681 ttctgaaggg tttccatgtt gagtaaagac tttttccgaa acatcggcgc tcagttcgta
     1741 ggtggccttc aattcgcggg tggcgtaacg gcgctcggtt ttgactgtct caaggaactg
     1801 ttccgtccgc ctttttacat tcgcctcacg ctggaacaaa tgttcctcat cggtgtgaaa
     1861 tctttttccc ttgtaaccat taccgccctt gcgactggtt ccgtcatggc tctccagttc
     1921 ggttatggtc tcgaaaaatt cggcggcaaa ctatacgtac ccaaagtcat ggcgctggcc
     1981 ttcgtacgtg aaatgggccc ggtgttcacc agccttctag tggcagcccg aattggttcg
     2041 ggtatcaccg cggaagtcgg ctccatgaat gtcacccaac aaatcgatgc aattcgcgca
     2101 cttgggacca accccattaa gaaaatcgtg ctacctcggt tgttagccac catgattacg
     2161 ctgccattac tcaccgttat ggctgatttt attggtcttt tcggtggcat gctcgtcacc
     2221 gtcaaagaat tggggcaaac acctgagttc tttttcacca aagcggtcga ggctcttact
     2281 ctaagcgact tcgtcatgag cctttttaaa ccctttgttt tcgcgatttt catagcactt
     2341 accgcgtgtt accgcggcat gcgcacgctg ggtggcaccc gtggtgtggg cgactccacc
     2401 acttgggtcg tggtcacaac gagcgttttt atcatggttt ccgatttttt cctcacaaag
     2461 gcctttattc tgttcgtacc ttcgccgaaa tagatacaga atagagaaaa atacatgagt
     2521 gttactgatg ctgaactaaa ccgcgaagta agccgacctg cgtctgtcgc acccgcgatc
     2581 ggaaaacccg tgctctcgct acgcaatatc gtgaagagct tcggctcaaa aaaggtac
//
LOCUS       DAOMXO010000324         2624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22418_length_2624_cov_3.180226, whole genome shotgun sequence.
ACCESSION   DAOMXO010000324 DAOMXO010000000
VERSION     DAOMXO010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22418_length_2624_cov_3.180226"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="PLH57_07310"
     CDS             <1..1726
                     /locus_tag="PLH57_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK02459.1"
                     /translation="MEAKNYIQALEYALVGAEPANAQNALSGEANKDLTPWVYRLAAA
                     ETLFLVGEYDRAERSYQLIVDANTAVSPEALFRLGEVYSARNFWERAVLSFEKAQTRY
                     PKLVNKYPSAMFNLAEAYFRIGKTDLAQKNFKEYIAKFPQDSSVWAANLRIAEIEHIH
                     LKSGDVTGGKKVKALYDHVVNNYPYTPGAELAQLRLSECSPVFSKNDPRREFYLGIFK
                     AEDLKVYHHDLVNLSEVEHWFNLAKARFGLAAAEFDLALSKADLYRTRFKSIPLGASF
                     AKVYHENVVASADYFAREKQFEKLLEIDAHFGDLVTEPKPISYLLPLLKANFEAGRLD
                     VAQRAIQGLASRVREMNAKERDGYYAIAARHEWLLSHDIEKTGEYLNKISDSGEFGPL
                     KLRMLAEVSLAAERYADVIATVGRLEQPSFRSKMSVGDQMEAEMFRLEALAKQSEYQR
                     VAKNANDFELRFGVYDEYANYLVRARDLRAAALYELKDYAAAIRAFSEILARADKHPR
                     RAEFEFKRARSLASVGREDEAKEGFQKIAKGPEDFGIWRKSAQTELEQLQWENDIKNQ
                     TKDRRSLQ"
     gene            1723..>2624
                     /locus_tag="PLH57_07315"
     CDS             1723..>2624
                     /locus_tag="PLH57_07315"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRK02460.1"
                     /translation="MIDIQTQDSRMKDITRLAETASQSKACVLVQGETGTGKGSIAKF
                     IHERSPRNGASFVTLNCAQQPEMLLETEIFGFEKGAFAGTVSEKRGKFEQATGGTLVL
                     DDVSELTPKLQAKVLKVLENNAVERIGSRAGVPVDVRIIAISNKNLADLVAQGKFSEA
                     LYYKLNGILLMVPPLRERMADIKAIVSQLMGRIATRSGRRVAGITEEAVQKLATHGFP
                     GNLRELEGVLEKAMMSAQDGVVRGSDIFVGQASVTSHQGSGAHSLDWEAEAQWAPGRT
                     LDQIERAVILKSLAFHNGNRTHTAR"
BASE COUNT          715 a          603 c          690 g          616 t
ORIGIN      
        1 catggaagcg aaaaactata tacaagcatt ggagtacgcc ttggttggtg ccgaaccggc
       61 gaacgcccag aatgcgctta gtggtgaggc gaataaagat ttaacccctt gggtttatcg
      121 cctagccgcc gcagaaacgc tattcttagt cggagaatat gatcgagcgg aacgttccta
      181 tcaattgata gtcgatgcaa acaccgctgt ctcgccagag gcgctttttc gcttgggcga
      241 ggtctactcg gctcgaaact tttgggagcg agcagttctg tcttttgaaa aagcgcagac
      301 gcggtatccg aagttagtga ataaataccc atcggcgatg tttaatttgg ccgaagctta
      361 ttttagaatt ggaaagaccg accttgctca aaagaatttc aaggaatata tcgccaagtt
      421 tccacaggac tcgtcagtct gggcggctaa tctaagaatc gcggaaatcg aacacattca
      481 tttaaagtcc ggtgatgtga ccggtggaaa gaaagtaaaa gctctgtatg accatgtggt
      541 gaacaattat ccgtacaccc ctggtgcgga actcgcccaa ttgcgactgt cggagtgttc
      601 gccggtattt tcgaaaaacg acccacgccg tgaattttat ttgggaatat tcaaagcaga
      661 agatctgaag gtctatcatc acgacttggt gaatttatct gaagtggaac actggtttaa
      721 tttagctaaa gcccgcttcg gattagcggc agccgaattc gatctcgcgt tatcaaaggc
      781 ggatctttac cgtacccgtt ttaagtcgat tccactcgga gcctcattcg ctaaggttta
      841 tcacgaaaac gtagtagcat ccgccgacta ctttgctcgt gaaaaacagt ttgaaaaact
      901 tctagagatt gatgcgcact ttggtgactt ggtaaccgaa cctaagccca taagttacct
      961 tttaccgctt ctcaaagcta attttgaggc cggtaggcta gatgtagccc aacgcgcgat
     1021 ccaaggtctt gcaagtcggg tacgggaaat gaacgctaaa gaacgcgacg gctattacgc
     1081 tatagcggct cgccacgaat ggctattgag tcacgacatc gaaaaaacag gtgagtatct
     1141 caataaaatt tcagactccg gagaatttgg cccgctcaag ttacgcatgt tggccgaagt
     1201 atcgctagcc gcggaacgct acgccgatgt tattgccacc gtgggtcgac ttgaacaacc
     1261 aagttttcgc tcgaaaatgt cggtcggcga ccagatggaa gctgagatgt tccgacttga
     1321 agcacttgcc aaacaaagcg aataccaacg ggtggctaag aacgccaacg attttgaatt
     1381 gcggtttggc gtgtatgacg agtacgcaaa ttatttagtg cgagcgcgcg atttacgtgc
     1441 cgccgccctt tacgaattga aagactatgc ggccgcgatc cgagcttttt cagagatact
     1501 ggctcgggca gataaacatc cgcgccgcgc agaattcgag ttcaagcgag ccaggagtct
     1561 ggcaagcgtt gggcgcgaag acgaagccaa agaaggcttt caaaaaatcg caaagggccc
     1621 cgaagacttc gggatctggc gcaaatccgc tcaaaccgaa ttagaacagc ttcaatggga
     1681 aaacgacatt aaaaaccaaa cgaaagacag gaggtcttta caatgatcga cattcaaact
     1741 caggactcga ggatgaaaga tataactcgc ttggccgaga ctgcgtcgca gtcaaaagcc
     1801 tgcgtactgg tgcagggtga aaccggtact ggtaaaggaa gcattgcgaa gttcattcat
     1861 gaacgctctc ctcgcaatgg agctagcttc gtgacgctca actgcgcgca gcagccagaa
     1921 atgctacttg aaactgaaat ttttggtttc gaaaaaggtg cgtttgccgg aactgttagc
     1981 gagaaacgtg gaaagtttga acaagcgacc ggtggcacgc ttgtactcga cgacgtttca
     2041 gagttaacgc cgaaattgca agcgaaggtg ttgaaggttc ttgaaaataa cgcagtagaa
     2101 cggatcggct caagagctgg ggtaccagtt gatgttcgaa tcatcgcgat ttcgaataaa
     2161 aatctggcag acctagtggc ccaggggaaa ttttcagagg cactctatta taaactcaat
     2221 ggaatcttgc tcatggttcc tcctctgcgc gaacgaatgg ctgacattaa agcgatcgta
     2281 tcccagttga tgggacgaat tgcaacacgc tcgggccgtc gcgttgcggg aattactgaa
     2341 gaagcagttc agaagcttgc gactcacggc tttcctggca atttgcggga actcgagggc
     2401 gtactcgaaa aagccatgat gagtgcccaa gatggtgtgg tgcgcggttc cgatattttc
     2461 gtgggccagg cgtcggttac ctcacatcaa ggttcgggcg cacactccct tgattgggaa
     2521 gcagaggctc aatgggctcc cggtcgtacg cttgatcaga ttgaacgcgc ggtgatcttg
     2581 aagtctcttg cgtttcacaa tggcaatcgt acccatacgg cgcg
//
LOCUS       DAOMXO010000325         2608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22632_length_2608_cov_3.972973, whole genome shotgun sequence.
ACCESSION   DAOMXO010000325 DAOMXO010000000
VERSION     DAOMXO010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22632_length_2608_cov_3.972973"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLH57_07320"
     CDS             <1..467
                     /locus_tag="PLH57_07320"
                     /inference="COORDINATES: protein motif:HMM:NF039287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRK02461.1"
                     /translation="LGNDFGNLLMRIVKLARKRLGDQVAPVNLVANTNVNFDFEKVHL
                     EMDRLMSAREHNRALDVLWSDLNKVNAYVNASTPWAIKDGDPQFQAVVHQTLQALYCA
                     SILLAPFMPHTSAEALRSLGFKDEDFSKINRKNVSSLTFQLSDPPVLFPRVA"
     gene            complement(503..2341)
                     /gene="glmS"
                     /locus_tag="PLH57_07325"
     CDS             complement(503..2341)
                     /gene="glmS"
                     /locus_tag="PLH57_07325"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRK02462.1"
                     /translation="MCGIIGYVGKKECSRFLVDGLKRLEYRGYDSSGIAISRNDQSNF
                     EVRKAEGKIERLEIALRNSPIQGAIGLGHTRWATHGKPNTINAHPHRAGTTVLVHNGI
                     IENFESIREELLARGASIVSETDSELFGHLVEEELKKTATFEEAVRLSFLKLEGSCSI
                     VVVNDRFPGKIVAVRTGTPLVVSRSQKADGCFVASDAQAIVEHTDEVTYLEPRDLVVC
                     TQSEFKIFPIDKKISPENEIKRSATKLDWKMADFDKAGFPHFMLKEIHEQPRAILDTL
                     DLLIERDTMRARMEEIGALVQRASKIQIIACGTAWHAGLVGKYLLESVARVPVEVDLA
                     SEYRYRDPVIDEHTLVLAVSQSGETADTLAALREAKRKGAMTAAICNVRGSSMPREVA
                     ATLFTAAGPEIGVASTKAFTTQILVFMMLGQFLAHARGVKTKAPDVHFFLQLPHQVQS
                     ALELAPLVQKISESCLQSRGYLYIARGRLFPIALEGALKMKEISYVHAEGYPAGELKH
                     GPIAMVESNMTVVVLAPRDELYEKTMSNLQEVKARGGRIVAVGDHDDFSLQKLSDHFI
                     GMRFAHDWSDSILATIPMQLLAYYTAVLKGTDVDKPRNLAKSVTVE"
BASE COUNT          657 a          650 c          620 g          681 t
ORIGIN      
        1 aactcggtaa cgatttcggt aatttgctga tgcgaattgt gaagctagcg cgtaaacgcc
       61 ttggcgatca agtggctcca gtaaacttag tggcaaatac gaacgtaaat tttgactttg
      121 agaaggttca tttagaaatg gatcgcctaa tgtcagcacg cgaacacaat cgcgcgctcg
      181 acgtgctttg gtcggacctc aataaagtga acgcatatgt taatgcaagc actccctggg
      241 ccattaaaga tggcgaccca cagtttcaag cggtggttca ccaaactcta caagcgcttt
      301 attgcgcaag tatcttgctt gcgcctttta tgccgcatac atcagccgaa gccttgcggt
      361 ctctaggatt caaagacgaa gatttttcaa agattaatcg caaaaacgtg agcagtttga
      421 cctttcagct ctccgacccc ccggtacttt ttccgcgggt tgcttgattg tagaatcggg
      481 catttgcacg cttcagtgat gactattcca ccgtgactga tttagctagg ttccgaggct
      541 tatctacgtc ggtccctttg agtacagcgg tgtagtaagc caagagctgc atcgggatgg
      601 ttgcgagaat agaatcgctc cagtcgtggg caaaacgcat tccaataaag tggtctgaaa
      661 gtttttgtaa cgaaaaatca tcgtgatcgc cgactgcgac aattcgacca ccgcgagctt
      721 ttacttcctg taaattcgac atggtttttt cgtaaagttc atcccggggc gccaacacga
      781 ccaccgtcat gttagactcc accatcgcaa tgggcccgtg ttttaattca cccgccgggt
      841 aaccctctgc atgcacgtac gaaatttctt tcattttcag agcgccttcg agcgcgatcg
      901 gaaacaaacg gccgcgcgca atatagaggt accctcgcga ctgcaaacag ctttccgaaa
      961 ttttctgcac caaaggcgcg agttccaagg cgctttgaac ttgatgaggt aactgtagaa
     1021 aaaagtgtac atcgggcgcc ttggttttta cgccgcgagc gtgcgccaga aactgtccga
     1081 gcatcatgaa aactaaaatt tgagtcgtaa atgctttggt agaggccaca ccaatttcgg
     1141 gacctgcggc agtaaataat gttgctgcaa cttcacgcgg catgctggag ccgcgaacat
     1201 tgcaaattgc tgccgtcatt gctccttttc gcttggcttc gcgaagcgcg gcaagcgtat
     1261 cggcggtttc ccccgactga cttaccgcga gcaccaaagt atgttcatct attactgggt
     1321 ctcggtagcg atactcgcta gccaggtcta cttccacagg tactcgagcc acgctttcaa
     1381 gcaggtattt gccaaccagt ccggcatgcc aagcggtccc acaagctatg atctgaattt
     1441 tcgaggctcg ttggacaagt gcaccgattt cttccattct tgcgcgcatc gtatcgcgtt
     1501 cgattagaag atccaaagtg tccaatatag ccctaggctg ttcgtgaatt tctttaagca
     1561 tgaaatgcgg aaaaccagct ttatcgaagt cggccatttt ccaatctagt tttgtcgccg
     1621 atctttttat ttcgttttca ggactaattt tcttatcgat tggaaaaatt ttaaattcgg
     1681 attgagtaca gaccactaaa tctcgcggct caagataagt gacttcgtct gtgtgttcta
     1741 caatcgcttg agcatcactt gccacgaagc acccgtcagc cttctgcgag cgcgacacta
     1801 ctaggggagt cccggtgcga acggcaacga tttttcctgg aaagcgatcg ttgaccacga
     1861 cgatgctgca ggacccctcc agttttaaaa atgaaaggcg aactgcttct tcaaaagtgg
     1921 cagtcttttt taattcttct tcgaccagat gcccaaatag ttctgagtcc gtttcgctga
     1981 caatcgaagc accgcgcgca aggagttctt cgcgaatgct ctcgaagttc tcgataatac
     2041 cattgtgtac cagcaccgta gtgcccgccc ggtgggggtg agcgttaatg gtgtttggtt
     2101 ttccgtgggt agcccaccga gtatgcccga gaccgatggc cccttgaatc ggagagtttc
     2161 ggagagcgat ctctaagcgt tcgatcttgc cttcggcttt gcgaacttcg aagttcgact
     2221 gatcgtttct tgagatcgca atgcccgagg agtcgtaacc ccgatattcg agccgtttaa
     2281 gcccgtcgac caaaaagcga gaacactctt tttttccaac gtatcctatg attccgcaca
     2341 tactggaacg cagcgtatgt cagaggggtg cggggtcgca aacgaaaata ttgcaatctg
     2401 ttgcaagccg gggattttca gttcagaata taagttcaaa atgagtacca aactcccaaa
     2461 aaagcatggc gaaaaaatta aaaccgataa gctttttcgc ccacaacagg ggggagtgtt
     2521 cgaacggcgt aaacacgatc gatttttggt tacccagcag ttttacagtc taaacctcac
     2581 tgcaaaaaag gccaacgacg aagacttt
//
LOCUS       DAOMXO010000326         2607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22644_length_2607_cov_5.309561, whole genome shotgun sequence.
ACCESSION   DAOMXO010000326 DAOMXO010000000
VERSION     DAOMXO010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22644_length_2607_cov_5.309561"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLH57_07330"
     CDS             complement(<1..598)
                     /locus_tag="PLH57_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRK02463.1"
                     /translation="MATVLIYAEHKEGKVKKVTHELISEANRAGAESVVFVAGQGLDA
                     VNKELGEMGAKRVIAAQGDAVKFYSNEAYGKLFVDAVKSTNPSVILIPATAQGKDLAP
                     RIAALLGVGLASDCTKIQIDGEKLKATRPIYAGKAVADVEFVGPKPYMATVRPNALSV
                     GAASPGASAAVTALSLDTGALKSTVKDVVRGATAKLDVT"
     gene            complement(623..1396)
                     /locus_tag="PLH57_07335"
     CDS             complement(623..1396)
                     /locus_tag="PLH57_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRK02464.1"
                     /translation="MNIFVCIKQVPATETKIKINGSGTGIDTTGVKWIMSPYDEFAVE
                     EALRLKEKNAGSTVTVFSAGPSRIAETLRTALAMGCDNAVCVDTPEEIDSFQAAKCLA
                     EAIRKEGKPDVIFTGKQAIDDDCAQVSQLLATFLELPYATVVLNAEYGSGQITVKRET
                     EGGTIEIIALPTPCLIAAQKGLNEPRFASLPNIMKAKKKEIKTHPMASLGVTDADRKI
                     KLVNMQLPPAKAAGKKLAGDAAAQSKDLVKLLREEAKVL"
     gene            complement(1476..>2607)
                     /gene="lysA"
                     /locus_tag="PLH57_07340"
     CDS             complement(1476..>2607)
                     /gene="lysA"
                     /locus_tag="PLH57_07340"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRK02465.1"
                     /translation="INRTRRLQKSLEPLEDRAGIRFAVKASSNIHILSLLTKLGCGAD
                     IVSGGELFRASLAQIPPDKIVFSGVGKTEEEISRAIVAGIELFNVESLSEVELLNVVA
                     QKHNTNVDIALRFNPNISAQTHRHISTGLKNSKFGLTSDEFWAAVTMSEKLPRIKLIG
                     ISCHIGSQITDLRPFRRAWEQLLILAKKVKHITTLDIGGGLGIDYQCPIESAALEDYG
                     KAIVTFFKSEPYKILVEPGRSLVGPTGVLVSKLLHLKKRTNRNIAVLDAGMNDLLRPA
                     LYGATHGIFPLRSRAPGAKSIVCDVVGPICESADLFLKSQPLPIWGSDSIYALGCAGA
                     YGMSMASQYNSRGRPPEVLVDRDQYRVIRRRETNLDLVNHEL"
BASE COUNT          627 a          651 c          615 g          714 t
ORIGIN      
        1 ctgtcacatc aagcttcgca gttgcaccac gtaccacgtc ttttacggta ctcttcaggg
       61 cgccggtgtc gagtgaaagc gcagttactg ctgcggaggc gccgggacta gccgctccta
      121 cggatagcgc gttcggacga accgtggcca tgtaaggttt tggaccaacg aattcaacat
      181 cggcaaccgc cttcccagcg taaatagggc gagttgcttt gagcttttca ccgtcgattt
      241 gaatttttgt acagtcactt gccagcccaa ctccgagtaa cgccgcaatg cgcggagcca
      301 aatctttacc ttgggcagtg gcgggaatta gaataacgga tgggttagta gacttcactg
      361 cgtcgacgaa cagttttcca taggcttcgt tggaataaaa ttttacagca tccccctggg
      421 ccgcaattac gcgtttggcg cccatttcgc ccagttcttt attgacggca tcgagtcctt
      481 gacccgctac aaataccacc gattcggcac ccgctcggtt cgcttctgaa attaattcgt
      541 gggtaacttt ttttactttg ccttctttgt gttcggcgta aatcaatacg gttgccattt
      601 gaaaatgctc ctatgaatgt aattagagaa ctttagcttc ttcgcgcaac agctttacga
      661 gatctttaga ctgtgcggcc gcgtcgcctg ccagtttctt tccggcagct ttggccggcg
      721 gcaattgcat atttactagc tttatcttgc gatcggcatc ggtcactccg agagaggcca
      781 tgggatgcgt tttaatttct tttttctttg ccttcatgat gttgggcagc gaggcgaaac
      841 gcggttcatt gaggcccttt tgcgcggcaa tcaagcaagg ggttggaagc gcaataattt
      901 caattgttcc cccttccgtc tcacgtttaa ccgtgatttg accactaccg tactccgcgt
      961 ttaataccac cgtagcgtaa gggagctcca aaaacgtggc caacaactga cttacctgag
     1021 cgcaatcatc gtcgatcgct tgtttgccag tgaagatcac gtcgggctta ccttctttgc
     1081 gaattgcttc agccaaacat ttagctgctt gaaaactgtc gatttcttct ggagtgtcta
     1141 cacacacagc gttgtcacaa cccatggcta atgcggtgcg cagggtttcc gctattctac
     1201 tgggaccagc actaaaaacg gtcacggtac ttcctgcgtt tttttctttt aaacgcaggg
     1261 cctcttcaac tgcaaactca tcgtagggcg acatgatcca ctttacgccg gtggtatcta
     1321 tgcctgttcc agaaccattg attttaattt tggtttcggt cgcggggacc tgtttaatac
     1381 acacgaaaat attcatttaa aactccccaa tagttagaca gtgtgacggt tcaacagtat
     1441 tcgattagat cgagtagtca atcttaaaac cggcgctaaa gctcgtgatt aacgagatcc
     1501 aaattagttt cacgccggcg aataacgcgg tactgatcgc ggtctaccaa aacctcgggc
     1561 ggtcgtccgc gcgagttata ttgactggcc atcgacatgc cgtaagcgcc agcgcaaccg
     1621 agtgcataaa tggaatcgga cccccaaatt gggagaggct gcgacttcaa gaataaatcc
     1681 gcgctttcgc aaatcggccc tacaacatca cagacgatcg atttagcgcc cggtgcacgc
     1741 gatcgcaggg gaaagattcc gtgggttgct ccatagagtg cggggcgcaa tagatcattc
     1801 atacccgcgt ccagcactgc aatatttcga ttggttcgtt ttttaagatg taaaagtttc
     1861 gacaccaaaa cacccgttgg gcctaccagc gacctcccgg gctcgactaa aattttatac
     1921 ggctcgcttt tgaagaaggt aacaatcgct tttccgtaat cttccaatgc ggcagattca
     1981 attggacatt gataatctat gcctaaaccg ccgccgatat cgagcgtagt gatatgtttc
     2041 acttttttag ccagaatcaa cagctgttcc caggctcgcc gaaagggccg aaggtcagta
     2101 atttgacttc caatatggca acttattcca attaatttga tgcgcgggag cttttcgctc
     2161 atggttaccg ccgcccaaaa ctcgtcagaa gttagaccaa acttgctgtt tttaagccca
     2221 gtagaaatgt gcctgtgtgt ttgcgccgaa atatttggat taaatcgcaa ggcgatatcg
     2281 acattggtgt tgtgtttttg agcgacaaca ttgagtagct cgacctccga aagtgactcg
     2341 acattgaaga gctcaattcc ggcgactatt gcgcgagaaa tttcttcttc agttttgccg
     2401 acgccggaaa atacaatctt atctggtgga atttgagcta acgaagctcg aaataattcg
     2461 ccgccactca caatatcggc gccgcatcct aactttgtta gtaacgacag tatgtgaatg
     2521 ttgctagaag ctttgacggc aaagcgtatt cccgcgcggt cttcaagtgg ttcaagagat
     2581 ttttgtaggc gacgggtacg gttaata
//
LOCUS       DAOMXO010000327         2604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22692_length_2604_cov_5.541389, whole genome shotgun sequence.
ACCESSION   DAOMXO010000327 DAOMXO010000000
VERSION     DAOMXO010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22692_length_2604_cov_5.541389"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLH57_07345"
     CDS             complement(<1..819)
                     /locus_tag="PLH57_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRK02466.1"
                     /translation="MKGGIGKYKQQQKIAKQAKETIALREELASLQEIVAVENGDSLT
                     AVSFDQVVLSEEAATRLADEAMAEDEDLDLEKIINVAKNSVARPKIDDRSGPDKKKKK
                     EKLGSREEIILEYAPLIKYVAQKIAARLPANIELDDLISSGVIGLMDAIEKYDPTRDN
                     KFKTYAEFRIRGAILDELRAQDWVPRSVREKGKMLERAFAKIEQERGRPATDEEVCEE
                     LKISQDEYYDLLNQVRSVSLLSYDDLSTVSKGDKRTALGMTENGKGANPYVEVNS"
     gene            complement(816..1742)
                     /locus_tag="PLH57_07350"
     CDS             complement(816..1742)
                     /locus_tag="PLH57_07350"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HRK02467.1"
                     /translation="MEQHGSGKRQDQADTLRRLMHGRVAIRPQTFKPIRVISVTSGKG
                     GVGKTNIVANLAVLLQRLGHKVLVLDGDFGLANLNIVMGLEPKGTLYDVLEGTKTIEE
                     VMLTGPGGIRVIPASSGIFKMTQLSMAEKAILMERLEQSPFSFDVLLIDTGAGINSDV
                     TYLNSAATEVIVVATPEPTSIADAYALMKVMSQDYRVKRFKLLVNMVANPKEGLGVYN
                     NILNVADRFLNISIEYLGHVLDDRKISQAVLSRKVACESYPTSLAVRCLETTAKSLID
                     TPAKGELTGNVQFFWRTLLKEDETSAVDRREL"
     gene            complement(1767..>2604)
                     /locus_tag="PLH57_07355"
     CDS             complement(1767..>2604)
                     /locus_tag="PLH57_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02468.1"
                     /translation="EELKNEQAMIADSRVAESAGDAVYAEFQNLLRNGIEKKYASLLT
                     KHVTLVLPREAQRDPDRVLDALAVELMNGIRIEQPLDVLSDQKKVLALVGATGVGKTT
                     TIAKLASQAILKKGLKVGLINIDSYKIAATDQLATYAKILGVPFRQAGTASELERALA
                     EFKPMDLVLIDTSGRSQRDTENLLEMQKILKSAGVESLLILSAPTRDQELYDILTRFS
                     IFDPKGVVFSKLDECTTYGCIYNVAQRSGLPLTYFTVGQRVPEDIEVASRERLASLIL
                     DL"
BASE COUNT          570 a          677 c          580 g          777 t
ORIGIN      
        1 gctgttaact tcgacgtacg gattcgcgcc tttgccgttt tcggtcattc caagtgcggt
       61 tcttttgtca ccctttgaaa cggtggacaa atcatcgtag ctcaacaggg acaccgacct
      121 cacttgattg agaagatcgt agtactcgtc ttgcgaaatt tttagctctt cgcaaacctc
      181 ttcgtcagtc gctggtcgcc ctcgttcttg ttcaattttc gcaaatgccc gttcgagcat
      241 ttttcctttt tcacgcaccg aacgcggaac ccaatcttga gcacgcaatt catcgagaat
      301 tgcaccgcgt attcgaaatt cggcatatgt tttgaacttg ttatcgcgag taggatcata
      361 cttttcgatg gcgtccatta gtccgatcac gccggaacta atgagatcgt ctaactcgat
      421 attcgccggt aatcgagcgg cgattttctg cgcaacgtat tttatgaggg gagcatattc
      481 cagaatgatc tcttcgcggc ttccgagttt ttcttttttc tttttcttat caggacccga
      541 gcgatcatct atttttggtc ttgctacgct attttttgca acatttatga ttttttcgag
      601 atctagatcc tcgtcttccg ccatggcttc atctgccagc cgagtggctg cctcttcgga
      661 aagaaccact tgatcgaaac tcacggcggt aagcgagtcg ccattttcga ctgcgacaat
      721 ttcttgaagc gaagctaatt cctcgcgcaa agcgatggtt tctttggcct gtttggcgat
      781 tttttgctgc tgcttgtact ttccaattcc gcccttcata gctctctcct atcgaccgcg
      841 ctggtctcgt cttcttttaa tagtgttctc cagaagaact gaacgtttcc ggtgagttcg
      901 cccttggctg gagtatcgat taagcttttt gcagtcgtct ctaggcaccg tactgcgagg
      961 cttgtgggat aagattcaca agcgacctta cgcgacagta ctgcctgact aatttttcga
     1021 tcatctagta cgtgaccaag gtattcgatg cttatattca aaaaccgatc ggctacgtta
     1081 agaatgttat tgtagacgcc caaaccttct ttaggattgg ccaccatgtt gactaacagt
     1141 ttgaagcgct tcactcggta gtcttggctc atgactttca ttagtgcata agcatctgca
     1201 atactggttg gctcgggcgt tgccacgaca attacttcag tggccgcaga atttaagtac
     1261 gtaacatctg aattaattcc cgcgccagta tcgatgagca atacgtcgaa cgagaacggc
     1321 gactgttcga gccgttccat gagaatggct ttttcggcca tggaaagctg tgtcatctta
     1381 aaaatgccgg aggaagccgg aatgactcga attccgccag gcccggttaa cattacttct
     1441 tcgattgttt tcgttccctc aagaacatcg tacagagttc ccttaggttc caagcccatt
     1501 acgatattca ggttcgcgag accaaaatcc ccgtctaata ccaaaacctt gtgccccaac
     1561 cgttgcagca agaccgccag gttcgctacg atgttcgttt tcccgacgcc tcccttgccg
     1621 ctagttactg agattacacg tattggtttg aacgtctggg gtcttatggc gacccgtccg
     1681 tgcatcagtc ggcgaagtgt gtccgcttga tcctgtctct taccgcttcc atgttgttcc
     1741 atcgcaatgc tctccttcgc cgttgtttac aaatctaaga tcaagctcgc caaacgctca
     1801 cgcgacgcga cttcaatgtc ttcgggcacc cgctgaccga cagtgaagta cgtcaacggt
     1861 aatccagacc tttgagcaac gttgtaaatg catccgtaag tagtacattc atcgagtttg
     1921 ctaaaaacca cgcccttagg gtcgaaaatg ctaaaacgcg tcaatatgtc gtaaagctct
     1981 tggtccctag tgggagcact caagatcagc aacgactcta cgcccgcgct ttttaatatt
     2041 ttttgcattt ctaaaagatt ctcggtgtct cgttgcgacc gaccgctcgt atcgatcaag
     2101 actaggtcca ttggcttgaa ctctgccaat gcccgttcga gttcactggc ggtaccggct
     2161 tgcctaaagg gtacgcccag aattttggca tatgtggcca actggtcagt ggccgcgatt
     2221 ttgtaggaat caatatttat taaacctact tttaaaccct tttttaagat cgcctgactt
     2281 gcgagctttg cgatggtcgt ggttttaccc actccagttg cgccaactag ggctaatacc
     2341 tttttctgat cactcagcac gtccagtggc tgttctatgc gaatcccgtt catgagttct
     2401 accgccaaag cgtcaagtac gcggtcggga tctcgctggg cttctcgcgg aagtaccagc
     2461 gtcacgtgtt tagtgagaag tgacgcgtat ttcttttcga tgccgtttct caggagattc
     2521 tgaaattccg cataaaccgc atcgcccgcg ctttctgcga cgcgagaatc tgcaatcatc
     2581 gcctgctcgt ttttgagttc ttca
//
LOCUS       DAOMXO010000328         2587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22911_length_2587_cov_4.073460, whole genome shotgun sequence.
ACCESSION   DAOMXO010000328 DAOMXO010000000
VERSION     DAOMXO010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22911_length_2587_cov_4.073460"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLH57_07360"
     CDS             complement(<1..940)
                     /locus_tag="PLH57_07360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRK02469.1"
                     /translation="MLNSEFPKHARVVVVGGGIIGCSVAYHLAELGWKDIVLVERDRL
                     TSGTTWHAAGLMVTFGSTSETSTEIRKYTRELYKKLEAETGQATGFKDVGFVQVATDA
                     DRLEEYRRVSAFNRWCGVNVQEVSPSQVKELFPLARVDDVLAGFYVKEDGRINPVDVT
                     MAIAKAARMRGVKIFEETAATGILKKKNYVTGVRTTRGDIQAEYVVNCAGMWSRQFGD
                     LAGVNVPAQAAEHYYLITEEFEGMTPNLPILEDPSSYGYFREEVGGLMIGLFEPVCAP
                     WKIEGVPQDFSFGEIEPDWDRMGPYLEKAMNRIPISM"
     gene            complement(968..2182)
                     /locus_tag="PLH57_07365"
     CDS             complement(968..2182)
                     /locus_tag="PLH57_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02470.1"
                     /translation="MVSFYIWTLVCVLSSVAWAELPESNPFQNTAQWVNRIDSTVTPI
                     SVYYASDTVPENSVQENGESVRETAQRTLSLLETAWDLSAKMGFAKPVAYNPIDPNRF
                     AGFLVAGNEMGVEAGNPLEIDGGFLIWPSYMSIAPWGIYAGERFASTIVHEFHHAVQA
                     VYDWEEPGSIYEMSSNYVQELFFPGENADAAIEIADFQARPEWAPHYYDDYKTYFMYG
                     SWLYLKFLELRYFEGKADFLVKLWEATAPIPGDRNPIWVEAIETLLPKGVTYADSIAA
                     FARWRTWLEPQARKDARRIGKSVLQSQVVPPPRIHYRFAVTGRRQSLESAGMQLFGSH
                     YIELQCKTSCKGAQVLVEFLNSDAAQTVYWKMQRINQTKSSAIVVVTPLPKKSAFDIA
                     NDSLIPYRITVR"
     gene            complement(2301..>2587)
                     /locus_tag="PLH57_07370"
     CDS             complement(2301..>2587)
                     /locus_tag="PLH57_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02471.1"
                     /translation="TVQKIDELKSQGENLGKERVEEISEIAETLRNDIQNGNADDKKR
                     ALEMLRYMYPENAEEKFRQLESARNAKEASEIVTPAEDEGSEDELYEGDE"
BASE COUNT          649 a          687 c          576 g          675 t
ORIGIN      
        1 tcatagaaat cggaatgcga ttcatagctt tttctaagta aggccccatg cgatcccaat
       61 cgggttcgat ttctccgaat gaaaaatctt gaggcacacc ctcaattttc catggtgcac
      121 aaacgggctc gaaaagcccg atcatgagcc caccaacttc ttcacgaaaa taaccatacg
      181 aagacgggtc ttctagaata ggaagattag gagtcatgcc ttcgaattct tcggtaatta
      241 aatagtagtg ctccgcagct tgcgccggaa cgtttacccc cgcgagatca ccaaactggc
      301 gcgaccacat gcccgcacag ttcacaacat attcagcctg aatatcgcct cgagtcgtac
      361 gcactccggt aacatagttt ttctttttca gaattccggt ggctgcagtt tcttcgaaaa
      421 tttttactcc ccgcatacgg gcagccttgg cgatggccat tgtcacatcg accggattaa
      481 ttcgaccgtc ttctttgacg taaaaaccag ccaacacgtc gtcgacgcgc gcgagcggaa
      541 aaagttcctt aacctgtgag ggtgaaactt cctgaacgtt cacgccacac caacgattga
      601 atgccgaaac gcggcgatat tcttcgagtc ggtccgcatc ggtcgcgacc tgtacaaaac
      661 ccacatcttt gaaaccggtt gcctggccgg tttcggcttc taatttttta taaagttccc
      721 gagtgtattt tcgaatctcg gtagaagttt cggaggtcga tccaaacgtc accatcaacc
      781 cagcggcatg ccaggtggta cccgatgtta aacgatcgcg ctcaacaagt acgatgtctt
      841 tccagcccag ctcggccaga tgataggcca ccgaacagcc gatgatcccg ccgcctacta
      901 cgacaactcg tgcgtgtttt ggaaattccg aattcaacat atatcccctc ttttgttggc
      961 tctaccctta cctcaccgta atgcgatagg gaatcagact gtcgttggca atgtcgaaag
     1021 ccgacttttt aggtaaaggt gtgaccacca caatcgctga acttttggtc tgattgatgc
     1081 gctgcatctt ccaataaact gtttgtgccg cgtcgctatt taaaaactcc actaaaacct
     1141 gggcgccctt gcagctcgtt ttgcattgaa gttcgatgta atgagatcca aatagttgca
     1201 tgcccgcgct ttcgagcgat tggcggcgcc cggtcaccgc gaaccgataa tgaatccgtg
     1261 gcgggggaac cacttgagac tggaggaccg attttccgat tcgccgagcg tctttgcgag
     1321 cttgtggttc aagccaagtt cgccagcgag caaatgccgc gatcgaatcg gcgtaagtaa
     1381 cacctttcgg caagagggtt tcgatcgcct ctacccaaat cgggtttcga tctcccggaa
     1441 ttggtgccgt cgcttcccac aacttcacta aaaaatccgc tttgccttca aaataccgca
     1501 actctaaaaa cttaagatac aaccagcttc cgtacatgaa gtaagttttg taatcatcgt
     1561 aatagtgggg cgcccattcc gggcgagcct gaaagtctgc aatctcaatg gcggcgtcgg
     1621 cattttctcc agggaaaaac agttcttgaa cataattaga actcatctcg taaatacttc
     1681 caggttcttc ccagtcatac accgcttgaa cggcgtgatg aaattcgtga acaatggtcg
     1741 atgcaaatcg ctcacccgcg taaattcccc atggtgcaat cgacatatac gaaggccaga
     1801 ttaaaaaacc gccatcgatc tcaaggggat taccagcctc tacacccatt tcgttccctg
     1861 ctaccaggaa cccggcaaat ctattgggat caattgggtt gtaagccacg ggtttggcaa
     1921 aacccatttt ggcagatagg tcccatgccg tttcaagcaa actcaacgtg cgttgggcgg
     1981 tttctcgcac ggattctcca ttttcttgaa cagaattttc tggcaccgta tctgacgcgt
     2041 aatagactga aattggcgtg acggttgaat caattcgatt cacccactga gcagtatttt
     2101 gaaaagggtt cgattccggc aactcggccc acgcaacaga actcaacacg cacacgagtg
     2161 tccaaatata aaacgaaacc ataggccact gcgtttgcct gcagcgggcg ccataaaaat
     2221 acacgtagtt ccccctaagc taacctgaaa gaggctatca aatgagtttg ggctggtttt
     2281 ggcaataaca aaaaaagacg ctattcgtcg ccttcgtaga gttcgtcttc tgaaccttcg
     2341 tcttcggcgg gagtcacaat ttcagatgct tctttggcgt tgcgagcgga ctcaagctgc
     2401 cggaattttt cttcggcgtt ttcagggtac atgtacctca acatttcgag cgcgcgtttt
     2461 ttgtcgtcgg cgttcccatt ctgaatatcg tttcggagag tttcggcaat ctcgctaatt
     2521 tcctctacgc gttctttacc tagattttcg ccctgacttt taagctcatc aattttttgt
     2581 acagttt
//
LOCUS       DAOMXO010000329         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22961_length_2583_cov_4.949367, whole genome shotgun sequence.
ACCESSION   DAOMXO010000329 DAOMXO010000000
VERSION     DAOMXO010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22961_length_2583_cov_4.949367"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..423
                     /locus_tag="PLH57_07375"
     CDS             76..423
                     /locus_tag="PLH57_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRK02472.1"
                     /translation="MRDIKSPWNGVRNYQARNYIRSMKPNDIALIYHSGKKPAIVGRG
                     TIVSAPYPEPVDGVSKEWTQVDVQFLDKLTQSIALDQLKNEPRLAQLKLFRQSRLSVV
                     PLTQEEYKILIAL"
     gene            485..922
                     /locus_tag="PLH57_07380"
     CDS             485..922
                     /locus_tag="PLH57_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRK02473.1"
                     /translation="MNSLELWRQTNPWRGLSNWSRVMDRIFEDTVPANGDANWFNPAA
                     KVEETPTHYEFSFDLPGVKKEEVKIELHKGQLTVAGERREEKRETSKRQHVLEQSYGS
                     FYRAFQLPQDVNAERVEAKFENGVLHITVAKGEEAKARQITVK"
     gene            1047..1892
                     /locus_tag="PLH57_07385"
     CDS             1047..1892
                     /locus_tag="PLH57_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRK02474.1"
                     /translation="MIKIRPSSAITLKSDTSIGEVVRVMRERKVGSLIITASTYPHQL
                     VGIFTERDLVRWVHEITSGGFWDKPISTIMTKSLITVHISEVDQAAEVMVKNNIRHLP
                     VVFTDDRGVQHLAGVLSMRDLFRRFVRGHTPTQELARGIESKSTANKKTTRIAVISND
                     RSAQAAIDGIVHQSKRYALQSIDLDALLSAVDGGREFPALDILIFDIDFAQTERWGFL
                     LKSILKIEDGPKVLLIFDPLLHSNAEISAIKTLTAFENFRAMPKPLNLLDFFKRLRIW
                     SGANG"
     gene            1885..>2583
                     /locus_tag="PLH57_07390"
     CDS             1885..>2583
                     /locus_tag="PLH57_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02475.1"
                     /translation="MARIAVIDLGTNSVRFDIHELAKSGNYKTLFREKLMVRLGQDVF
                     TKGRLNNQAVQRTVLAFKAFHKVIQSLHVKRVVAFGTSALREASDAPRLINAIARETG
                     IVLKVISGAEEARLISLGVLQNERLGTGQKKFALIDMGGGSTEISLCKKNKTYFSVSL
                     PLGTARLQQIFLRRIPPSPESVQSLREEIRKSLKTALAASKVLHPPIATLIGSSGTVR
                     ALAKVLREREGGRTF"
BASE COUNT          725 a          655 c          575 g          628 t
ORIGIN      
        1 gaataaagtt tagattttcc gctatgcagt actggattct aaaaactgaa cctgaaacat
       61 ttagtataga cgaccttgcg cgacataaaa tcaccttgga acggcgtccg aaactatcag
      121 gcgcgcaact acattcgttc aatgaaaccc aacgacattg cactcattta ccatagcgga
      181 aaaaaaccag cgatcgttgg gcgaggcacc atcgtgagcg ccccctaccc cgaaccggtc
      241 gacggtgtgt cgaaagaatg gacacaagtc gatgtccaat ttctagacaa attaacgcaa
      301 agcatcgccc tagaccaact caaaaacgaa ccgcgactcg cgcagcttaa actgtttcgc
      361 caatcccgcc tttcggtggt gccacttacc caagaagaat acaaaatttt gatcgccctt
      421 taactcgcac cacttgacac ccataaacac gactccatct tttttgcatg gaggcacgaa
      481 aactatgaac tcactagaac tttggagaca aactaatcct tggcgcggac tttctaattg
      541 gagtcgcgta atggatcgta tttttgaaga tacagttcca gcaaatggcg acgctaactg
      601 gtttaatccc gccgctaagg tagaagaaac accaacgcac tacgaattta gtttcgattt
      661 gcctggtgta aaaaaagaag aggtaaaaat cgagctacac aaaggccagc tcaccgtggc
      721 gggcgagcga cgtgaagaaa aacgcgaaac gtctaagcgt caacacgtgc tggaacaaag
      781 ttacgggtca ttttaccggg cttttcaact ccctcaggac gtaaatgccg agcgcgtcga
      841 agcgaaattc gaaaacggcg tactccacat tacggtcgcc aagggcgaag aagcaaaagc
      901 acgacaaatt acagtcaaat agcgcgtaaa accccccctc gtttggcgct attttaaaaa
      961 gaagacgggc ttcccagcaa atgtgggagc ccgttttttt tttggacctt tcgggacgct
     1021 gatctatttt gtatcattta gtcaccatga tcaaaattag accgtcctct gccattactc
     1081 taaaatcgga cacctcgatc ggagaagtgg tgcgcgtgat gcgagagcgc aaagtcggct
     1141 cactcatcat caccgcctct acttatccgc accaactggt aggtattttt acagaaaggg
     1201 acctcgtccg ctgggtgcac gaaattacct ctggtggttt ctgggacaaa ccaatttcta
     1261 ccatcatgac taaatctctc attacggttc atatttccga agtcgatcag gcagcagagg
     1321 tcatggttaa aaacaacatc cgccatcttc cagtcgtgtt caccgacgac cgaggagttc
     1381 agcacttggc gggcgtactt tccatgcgtg acctatttag acgcttcgtt cgagggcaca
     1441 ctcctaccca agagctcgcc cgtggtattg agtcaaaatc cactgctaat aaaaagacca
     1501 ctcgcatcgc cgttatttct aatgatcgta gcgcgcaggc ggcaatcgac ggaatcgttc
     1561 accagtccaa acgctacgca ctgcagtcga ttgatctcga tgcgcttctt agcgcggtcg
     1621 atggcgggcg agagtttccc gcgttggata ttcttatctt cgatatcgat ttcgcacaaa
     1681 ccgaacggtg gggttttctt ttgaaaagca ttttgaagat cgaagatggt ccgaaagttt
     1741 tgcttatctt tgatccgctg cttcattcta atgctgaaat tagcgcaata aaaacattga
     1801 ccgcctttga aaactttcgg gctatgccaa aacctctaaa cctgttggac ttttttaaac
     1861 gattgcgaat ttggagtggg gctaatggct agaatcgcag tcatcgacct cggcactaat
     1921 tctgttcgct tcgacattca tgagttagca aaatcaggaa attacaaaac cctatttcga
     1981 gaaaaactta tggtccgcct cggccaggac gtttttacta agggtcgact aaataaccag
     2041 gctgttcagc gaacggtttt agctttcaag gcttttcaca aagtaattca aagtctccat
     2101 gtgaaacgag tagtcgcatt tggcaccagc gcacttcgcg aggcatcaga cgcccctaga
     2161 ctcataaacg caatcgctcg cgaaaccggc attgtgctca aagtaatttc cggtgccgaa
     2221 gaagcccgtt taatttctct tggagttttg caaaacgaac gcctgggcac cggtcaaaaa
     2281 aaattcgcac ttatcgacat ggggggcggg agtacggaaa ttagtctttg taaaaaaaat
     2341 aagacctatt tcagcgtgag ccttcctctt ggtactgctc gattgcaaca aatatttctt
     2401 cgtagaattc cgccgtcgcc cgaatcagtt cagagcttac gcgaagaaat tagaaaatct
     2461 cttaaaaccg cactcgcagc cagtaaagtt ctgcatccgc caattgccac cctaatcgga
     2521 tccagcggca ctgtgcgcgc gctcgcgaag gtgcttcgag aacgcgaagg cggccgcacc
     2581 ttt
//
LOCUS       DAOMXO010000330         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_22965_length_2583_cov_3.831883, whole genome shotgun sequence.
ACCESSION   DAOMXO010000330 DAOMXO010000000
VERSION     DAOMXO010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22965_length_2583_cov_3.831883"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLH57_07395"
     CDS             <1..472
                     /locus_tag="PLH57_07395"
                     /inference="COORDINATES: protein motif:HMM:NF036827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRK02476.1"
                     /translation="SSEKLVIEAPQPAKRTSSLHAVGVLSERPNGLVLLDDANPAPES
                     ASSKKKTAVKEKKVTQFSGKYELPPLSFLNHPEVSNHTLDRERLVENSKILEQKLTDF
                     GIEGSVVAVRPGPVITMYEFKPGPGVKISQIAALADDLSLALSAQSVRIVAPIP"
     gene            479..1684
                     /locus_tag="PLH57_07400"
     CDS             479..1684
                     /locus_tag="PLH57_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRK02477.1"
                     /translation="MVGIEIPNDEREKVYLREILSTEEFQGSNQTIPLAVGKDIAGTP
                     VTTDLARMPHLLIAGAPGAGKSVFINSVICSLLYKFTPHDMRLLMVDPKQLELNLYDN
                     IPHLLLPVVDDPKKASTALKWAVNEMERRYKIMAKLGVRNLAGFNEKLENDGPEKTIK
                     TLCPMTDDGMPESESLSHLFEHDSNGKPVIERMPFVLVIIDEFADLMMVAPKDIETSV
                     ARLAQKARAAGIHLIIATQRPSVDVITGLIKANLPSRISFQVASKMDSRTILDSIGSE
                     KLLGQGDMLFIPPGLSRLTRIHGAFVKEDEIEKICDHWRSQGAPVFKEEILLAPDEAN
                     AEEADGAGDELYQQAITIVRELGQASASMLQRRLKVGYNRAARMIEAMEMQGIVGPAD
                     GARPREVLY"
     gene            1832..>2583
                     /locus_tag="PLH57_07405"
     CDS             1832..>2583
                     /locus_tag="PLH57_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRK02478.1"
                     /translation="MRHGRARKGWVLALFSLIFTLTQPALVFADAPGEDADREQTPPT
                     ENSWEPILRTGYVTAISDGDTIRFRDDLSYEDDEDLRVRFVSIDTPEIWHNRLWVGQE
                     PWGQHATKYLAWLIPVGTRVSLVDFGKDRYGRHLGWVIYRNVNINYRMVKDGWATPYI
                     ICDGPRCNEEFFQRERVRDYFAACDEARSKELGVFNPENPMLELPFEYRNRINKEAPK
                     RWVADIDTMTLYRPEEYTYVDVCRRVFFRTVK"
BASE COUNT          709 a          639 c          626 g          609 t
ORIGIN      
        1 atcgagtgag aagcttgtga tcgaggcccc ccaacccgct aagcgaactt ctagccttca
       61 tgcagtaggt gttttgagcg aacggcccaa tggcttggtg ctgttagatg atgcgaatcc
      121 tgctccggaa agcgcttcga gcaagaaaaa aaccgccgtc aaagaaaaaa aggtcacgca
      181 gttttcaggc aaatatgaat tgccgccgct tagcttttta aatcacccag aagttagcaa
      241 tcacacttta gaccgcgaac gtttagtcga aaattctaag attttagagc aaaaactaac
      301 ggacttcgga attgaaggaa gcgttgtagc cgtccgaccg gggccagtaa ttacgatgta
      361 cgaattcaaa cctggcccgg gcgtaaaaat atctcagatt gccgcattgg ccgacgattt
      421 atcattggcg ctgtctgcgc agagtgttcg aatcgtggca ccaatcccct gaaaaagcgt
      481 ggtgggcatc gaaattccca atgacgaacg cgaaaaagtt tacttaagag aaatcttatc
      541 gaccgaggag tttcaaggaa gcaaccaaac aatccctctg gcagtcggaa aagacatcgc
      601 tggaactccg gtaactaccg acttggcgcg catgccgcat ttattgatcg cgggtgcgcc
      661 cggtgccgga aagagcgttt tcattaattc ggtcatttgt tccttgctgt ataaatttac
      721 acctcacgac atgcgactgc taatggttga tccaaagcag cttgaattaa acctttacga
      781 caacattcct caccttttac ttcccgtggt cgacgacccg aagaaggctt caacggctct
      841 gaagtgggca gtgaatgaaa tggaacgtcg ttacaaaatt atggcaaagc tcggcgtgcg
      901 caatctagca ggctttaacg aaaaactcga aaacgatggc ccagaaaaaa ccattaaaac
      961 tctctgccca atgactgacg atggaatgcc tgaatccgaa agcctctcac atttattcga
     1021 acacgactcc aatggaaaac cggtcattga acgcatgccg tttgtacttg ttatcataga
     1081 cgagtttgct gatttaatga tggttgcgcc caaagatatt gaaacaagtg ttgcgcgttt
     1141 agcgcaaaaa gctcgagccg cgggcattca tttaattatt gcaacccagc gcccgtcggt
     1201 cgacgtgatc actggtttaa ttaaagcgaa tctaccgagc cgaatttctt ttcaggtcgc
     1261 ttcaaaaatg gacagtcgca ccattctcga tagtattggg tccgaaaaat tactcggtca
     1321 gggagatatg ctttttattc caccgggcct ttcgcgctta actcgaattc atggcgcttt
     1381 cgttaaggaa gacgaaatcg aaaaaatttg tgatcactgg cgatctcagg gagcgccagt
     1441 gtttaaagaa gaaattctac tcgcacccga cgaagcaaac gcagaagaag ccgacggtgc
     1501 gggcgacgaa ctttaccaac aagccattac catcgtgcgt gaactgggcc aagcctccgc
     1561 cagcatgcta cagcgccgct taaaggtcgg gtataatcgc gcggcgcgca tgattgaagc
     1621 gatggaaatg caaggtatcg tgggaccagc cgacggcgcg cgtccgcgag aagttctcta
     1681 ctaaacccct agtgcggcct caccaactaa atgggagcgc ttcgacgctc cgcgattaaa
     1741 atattttcga gagggatttg gaatttcgtg ttttcgcgtt tagattttcc tcattacaca
     1801 acggttccac ccaacgacca aggaggttgc catgcgacac ggacgagcgc gcaagggatg
     1861 ggttctagct cttttttcat taatcttcac actaactcaa cccgcgctag ttttcgccga
     1921 tgctcccggc gaagatgcag atcgagaaca aactcccccc accgaaaact cttgggaacc
     1981 gattcttcga actggttacg taaccgcgat ttcagatggc gacaccattc gattccgcga
     2041 cgacctttcg tacgaagacg acgaagactt gcgggtgcga ttcgtaagta tcgacactcc
     2101 agaaatttgg cataatcgcc tctgggttgg acaagaaccc tggggtcaac atgcgactaa
     2161 gtacctggca tggcttattc cagtagggac acgcgtttca ctagtggact tcggcaaaga
     2221 tcgctacggt cgccatttgg ggtgggtcat ttatcgtaac gttaacatca actaccgaat
     2281 ggtaaaggat ggttgggcca ctccttacat tatttgcgat ggtcctcgtt gcaacgaaga
     2341 gtttttccaa cgcgaacgag ttcgagatta tttcgcagcg tgcgacgaag cgcgatccaa
     2401 agagctcggt gtatttaatc cagagaatcc catgcttgaa ctaccctttg aatatcgcaa
     2461 tcgcataaac aaagaggcgc caaaacgctg ggtcgccgac atcgacacca tgactcttta
     2521 ccgtccggaa gaatacactt acgtagacgt gtgccgcaga gtattcttcc ggacggtaaa
     2581 gag
//
LOCUS       DAOMXO010000331         2559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_23271_length_2559_cov_3.775160, whole genome shotgun sequence.
ACCESSION   DAOMXO010000331 DAOMXO010000000
VERSION     DAOMXO010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23271_length_2559_cov_3.775160"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1128)
                     /locus_tag="PLH57_07410"
     CDS             complement(106..1128)
                     /locus_tag="PLH57_07410"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRK02479.1"
                     /translation="MDKSLHDAGRLLAAAHSVIICTHLMPDGDGLGAEVALFHCLKRL
                     GKDVRIVNPDPVPGRYRFLDRGGVIETLGAGFKEAPFDLAVVVDTNEPKRLGKVWDFF
                     ERQAKTILFLDHHHGGLNGTNGKVHTVVDVRSSSIGELLYRLFHQAQDVLDPDAPSFR
                     LSPEMALGLYVSIITDTNSFRYSRTTALSHRIAAELIDFGLQPEEVYQNIYSSKSAAH
                     VRLIGEVLSRIEEVPLRSPSHENGTLAWVEIPRDVRKKFNASSDDTQSIVNFLLLLKN
                     AEILILLREEDDGRIKGSIKSKGTLVVNTVAQELGGGGHEFAAGFIVSGTMSEIRDRL
                     LRKLAA"
     gene            1246..1557
                     /locus_tag="PLH57_07415"
     CDS             1246..1557
                     /locus_tag="PLH57_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02480.1"
                     /translation="MSESKVDPLYIIFEKHLYDFHEEEENSKEFIDNIIKDYLNHLTS
                     RNVAVPQQWHGVVVEELREQVRNMLVKKTYGCLSISEFIKKEVAKKDQAKTKKLRVGR
                     K"
     gene            complement(1566..>2559)
                     /locus_tag="PLH57_07420"
     CDS             complement(1566..>2559)
                     /locus_tag="PLH57_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02481.1"
                     /translation="KGAGSYNQRLYELSKKNNLTGNRALDLLLRDPKITSFFDTSLSN
                     KEIEETLIAALGRGWPEIRKNSLKLIGDIISVKEGIGTNLLRAYYDEILNEKSFSSAL
                     DMIGKIPTNISNHPPDLRKKRSKMLTDLAVRNFQQKKRMLQRTDPAGSVHSWELDTLT
                     KIQDLVGEQLDSDDVKNFFSLMKNNSYFTLGRRKFASDKDLLLERLVLSAAREHMGNS
                     KIRKLVVEHLAMTGGLSRRPIIISVRTTDLGINLLPSELERILDVWFSKKLVSRDHIA
                     ESVFDFLIHSKILDVSTRVRLVLLLDKATDPSSFANKMLLSLRLQIVLPNLICD"
BASE COUNT          744 a          591 c          518 g          706 t
ORIGIN      
        1 gtaaaagact ttcctgcttt aagaattcgc cccatggaag tagacgctca cgtccgctta
       61 ttcgctgcaa caccaacccg atttcagaat tttttttgtc ttttttcacg ctgccaactt
      121 tctcaagaga cggtcgcgaa tctcgctcat agttccgctt acaataaaac ccgcggcaaa
      181 ttcatggcca ccgccgccca gctcctgagc tacggtgttt acgaccagag tgccctttga
      241 cttgatactc cccttgattc ttccatcatc ttcttcacga agcaaaatta gaatttcggc
      301 gttttttaaa agcaaaagaa aattaacaat cgattgagtg tcgtcgctgg atgcgttgaa
      361 tttcttgcgt acgtctctcg gtatttctac ccaagctaat gtcccgtttt cgtgactagg
      421 actacgaagc ggaacttctt caattcgact caaaacttcg cctattaacc gcacatgggc
      481 cgctgacttt gaagagtaga tattttgata cacttcttct ggctgtaatc cgaagtctat
      541 gagttcagcg gcaatacgat gagaaagtgc ggtggtacgg gaatatctaa aagaatttgt
      601 atcggtaata attgaaacat acaatccgag cgccatttcg ggtgatagtc taaacgacgg
      661 tgcatcggga tcaagaacgt cttgtgcctg gtgaaacagc cgatacaaaa gctcaccaat
      721 tgaggacgaa cgcacatcca ctacggtgtg cactttcccg ttggtaccat tgaggccccc
      781 gtggtgatgg tcgagaaata aaatcgtttt ggcctgacgt tcaaaaaagt cccacacttt
      841 gcccagtctc ttgggctcat tagtgtcaac tacgactgcg agatcaaacg gtgcctcttt
      901 aaagcccgcg ccaagagtct caattactcc cccccgatcc aaaaaacggt accgtccggg
      961 gactggatcc ggattaacga tccgaacatc tttacccagc ctttttagac aatggaatag
     1021 cgccacttcg gcccctagac catcgccgtc aggcataaga tgcgtgcaga tgataacaga
     1081 atgagctgcc gcaagtaacc tgccggcatc gtgtagtgac ttgtccattc ttcagcttat
     1141 cggaacaaga tataagattt gggataggta acatttgccg cttcaccctc tccaggggta
     1201 aggccttgtt accgataaaa tccttgggac cacaaaggag tgttaatgag cgagtccaaa
     1261 gtcgatccac tatatataat atttgaaaaa catctctacg attttcacga agaagaagag
     1321 aattctaaag agtttatcga caacatcatc aaagattatt taaatcactt aacttcgcgc
     1381 aacgtcgcgg tcccacaaca gtggcacggc gtcgtagtag aagaacttcg agaacaagtg
     1441 cgcaacatgc tggtgaaaaa aacctacggc tgtctttcga tttcggaatt cataaaaaaa
     1501 gaagtagcga aaaaagatca agcgaaaact aaaaaacttc gtgttgggcg aaaataatcg
     1561 gaacatcaat cacagattag attcggaaga actatctgca agcgaagact cagtaacatc
     1621 ttattggcaa aagaactagg gtcagttgcc ttgtctagta aaagcactaa gcgcacccta
     1681 gtagacacgt ctaatatttt ggaatgaatt aaaaaatcaa aaacactctc ggctatgtga
     1741 tctcgactaa ctagtttttt actgaaccac acgtctagaa ttctctcgag ttcagaaggc
     1801 agtaaattaa tacctaaatc agttgtgcga acacttataa taatcggtct acgggacaac
     1861 ccaccggtca tcgccaaatg ctctacaacc agtttacgaa ttttcgaatt tcccatatgt
     1921 tctcgtgctg cactcaaaac caagcgttcc aaaagtaagt ctttatcgct ggcaaactta
     1981 cggcgtccca aagtaaagta agagttgttt ttcattaagg aaaaaaagtt cttaacatca
     2041 tccgagtcaa gttgctcccc caccaggtcc tgaattttcg tcagggtgtc tagttcccaa
     2101 gaatgaactg atcctgcggg atccgtgcgt tgaagcatac gttttttctg ctgaaaattt
     2161 cgcacagcta agtcggttaa cattttgcta cgtttttttc ttaagtctgg cgggtgattt
     2221 gaaatattcg ttggaatctt gccgatcata tcaagtgctg acgaaaaact tttttcattg
     2281 agaatttcat cataataggc acgcaagaga ttagttccga tgccttcttt aacagaaata
     2341 atatcgccaa tcagtttcag tgaatttttt cgaatttccg gccaacctct ccctagggca
     2401 gcaatcaatg tttcttctat ctccttgtta ctcaacgaag tgtcgaagaa cgaagtaatc
     2461 tttgggtctc taagtaaaag gtctaacgct cgatttccgg ttaaattatt tttttttgaa
     2521 agttcgtaga gcctttgatt gtaggaaccc gccccttta
//
LOCUS       DAOMXO010000332         2552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_23342_length_2552_cov_4.301161, whole genome shotgun sequence.
ACCESSION   DAOMXO010000332 DAOMXO010000000
VERSION     DAOMXO010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23342_length_2552_cov_4.301161"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="PLH57_07425"
     CDS             <1..1320
                     /locus_tag="PLH57_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRK02482.1"
                     /translation="NTLLTPDDAFYNAIQWRDLADWKTELAPHYQTAKQMLGVAPNPK
                     NWLADEKLLEVAKEMGREHTFKKVDVGIFFGKPGEEGKTVPDPYFGGKGPARTGCLYC
                     GACMVGCRHGSKNTLDKNYLYFAEKNGAEIRAESNVVDIRALASGGYEVDVEKSTAWF
                     FKNRYTLRAKNVVLSAGVLGTVNLLLKCKLLTGGLPNVSDRLGQDVRTNNEALIGVTT
                     RDVSQDHSRGIAITSGFYPDDNTHVEPVRYSKGSSFMRLLAAPMADEGTWITRPLKLI
                     AATVLHPLDTLKLILNTRWAESTIILLVMQNIDNTMRFKLGRGLLTFFRKRLTTEVDP
                     TQKIPSYIAIGHTVARALARKIDGIPQSAVNEVMLQIPTTAHILGGCGIGKDATTGVI
                     STRHEVFGHPGLYVCDGSMIPANLGVNPSLTITAMTERAMDQIPSKK"
     gene            1395..>2552
                     /locus_tag="PLH57_07430"
     CDS             1395..>2552
                     /locus_tag="PLH57_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02483.1"
                     /translation="MSGWGTFPVNLTRIARLAIVMIIALWSEQAVAASGACGLDELIE
                     RASQINKAYTRVDVRNVAMNDLYAAAKATGNPDGSFQRPWVRIRLKDSDESIFVEMAQ
                     YSGPGEKKIALLFRDPSNTHYAEGRVYSSARLADEIESFEILNPGYYYNHPVTNLQPV
                     YKGVPWKKTKMPEEQLLDILSRNATPTNPNSNAPRAGKIVAATLTDNSVVIGELRATR
                     SSKVYSIGGKDYVVSDLRSVELSSADEPVYALRVLREMLEDGKTRTAKERIVRIELND
                     GSIVEGKVLDIDRVVESGKEQYKVSIASKETEEIGDGLYRDIVEDRRINPKNITRITE
                     RSSNIETNTAVFNHTTSAQRKAMADFLDMKPEEFEYIHDEAFYRLTSLKRRM"
BASE COUNT          705 a          653 c          632 g          562 t
ORIGIN      
        1 aatacgctcc tcactcctga cgacgcgttt tacaacgcaa tccagtggcg cgatctcgcc
       61 gattggaaaa ccgagttagc accacattat caaacggcca aacagatgct gggagtggcg
      121 cccaacccaa agaactggtt ggccgacgaa aagttgctcg aagttgccaa agaaatgggg
      181 cgtgagcaca cgtttaagaa agtggatgtt ggtatttttt tcggcaagcc gggcgaagag
      241 ggtaaaaccg ttccagaccc ttacttcgga ggcaagggac cagcccgcac cggttgtttg
      301 tattgcggag cctgcatggt gggctgtcgc cacggttcca aaaatacgct cgataaaaac
      361 tatctttatt ttgccgaaaa aaacggtgcc gaaattcgcg ccgagtcgaa cgtagtggat
      421 attcgagcac ttgcgagtgg tggctacgaa gtagatgtcg aaaaatccac cgcttggttt
      481 tttaaaaatc gctacaccct acgagctaaa aacgtagtcc tctcggcggg agtgctgggc
      541 acggtaaatc ttttgctcaa gtgcaaactc ctaactggag gtcttcccaa cgtgtcggac
      601 cgtttgggtc aagacgtgcg caccaataac gaagccctca ttggggtaac cactcgcgat
      661 gtttcgcagg atcattcccg ggggatcgca attacgtctg ggttctaccc agacgacaac
      721 actcacgtag aacctgtgcg ttactcgaaa gggtcgtcgt ttatgcgcct actggcggct
      781 cccatggccg acgaaggaac ctggatcact cgacccctca aactaattgc ggccaccgtt
      841 ttgcacccac tcgacaccct aaaattaatt ttaaataccc gatgggccga gagcacgatc
      901 atcttgctcg ttatgcaaaa catcgacaac accatgaggt ttaaactcgg gcgcggatta
      961 ctcacctttt ttagaaaacg cttaacgacc gaagtggatc caacgcaaaa gatcccctcg
     1021 tacattgcga tcggacacac cgtcgcacgg gcgctggctc ggaaaatcga cggcattccg
     1081 cagagtgcag tgaatgaagt tatgctgcag attccgacta ctgcccacat attgggcggc
     1141 tgcggcatcg gtaaggacgc aacaactggc gtgatcagta ctcgacacga agtgtttggc
     1201 catccgggac tttacgtgtg cgacggctca atgattcctg ccaatttagg ggtcaacccc
     1261 agcttaacaa taactgctat gaccgagcgg gccatggacc agataccctc taagaaatag
     1321 gtctatctat ttgatataaa tagataattt cacccattca tatttgacta aaaaaatcaa
     1381 ttatatgcgc tataatgagt ggctggggga cgtttccagt gaatcttacg cgcatcgcac
     1441 gcttggcgat cgtcatgatt atcgcactgt ggtcagagca ggctgttgcc gcgtctggtg
     1501 cttgcggcct tgatgaactc atcgagcgcg catcgcaaat aaacaaagcc tacactcggg
     1561 tcgacgtgcg gaacgtggcc atgaacgacc tttacgctgc ggccaaagcg accgggaacc
     1621 cggacggcag ttttcagcga ccttgggtcc gtatacgtct taaagactct gacgaatcaa
     1681 ttttcgtaga aatggcgcag tactccggac ccggtgaaaa gaaaattgca ctcctattcc
     1741 gcgatcccag caacactcac tatgcagaag gacgcgtcta cagttcggcg cgactcgcag
     1801 acgaaataga atcttttgaa attttaaatc ctggttacta ctacaaccat ccggttacca
     1861 acctccaacc ggtgtacaaa ggggtaccgt ggaaaaaaac caaaatgcca gaagagcaac
     1921 tactcgacat tttgagtaga aatgcgactc ccaccaatcc gaattcaaat gctccccgag
     1981 ctggaaaaat tgttgcagct actttgaccg acaacagcgt ggtcatcgga gaacttcgcg
     2041 ctacccgttc gagcaaggtc tacagcatcg gtggcaagga ttacgtagtc tctgatctac
     2101 gttctgtgga actgagtagc gctgacgaac ccgtctacgc acttcgagta ttacgcgaaa
     2161 tgctagagga cggaaaaacc agaacagcca aggaacgcat tgtgcgcatc gaactgaacg
     2221 acggttctat cgtagaaggt aaggtgttag acatcgaccg cgttgtcgag tctggtaagg
     2281 aacaatacaa ggtaagcatc gcatccaagg aaaccgagga aattggcgac ggactttatc
     2341 gggacatcgt cgaagaccga agaatcaacc cgaaaaacat tacgcgcatc actgagcgta
     2401 gcagcaatat cgaaacgaat actgcggttt tcaaccatac gacctcggcg cagcgaaagg
     2461 ctatggccga ttttttggac atgaagccag aagagttcga atatattcac gacgaagcct
     2521 tttaccgact aacttcgctc aaacgtcgta tg
//
LOCUS       DAOMXO010000333         2551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_23359_length_2551_cov_5.221154, whole genome shotgun sequence.
ACCESSION   DAOMXO010000333 DAOMXO010000000
VERSION     DAOMXO010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23359_length_2551_cov_5.221154"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1732)
                     /gene="ade"
                     /locus_tag="PLH57_07435"
     CDS             complement(53..1732)
                     /gene="ade"
                     /locus_tag="PLH57_07435"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRK02484.1"
                     /translation="MTSGHYTETLQAARGIIPAQLLLKNAKLLEVHSGQWVHTDIAVH
                     HGRVVGLDSGYTAHRTIDLKGAAVVPGFIDAHVHIESSCMVPGRFEQAVLARGTTSAI
                     CDPHELANVLGVEGLTYFLDCAEKTNLDLHVMLSSCVPATHLETNGGGTITAAQLAAL
                     KDRSRALGLAEVMNVPGVLNRSPEVHAKLEAFSDRPIDGHAPQLRGKDLSAYAACGIS
                     SCHESSELSEAQEKLLKGMAVWIREGSVAKDLDALLPILNAYSSATVGFCTDDRNPLD
                     IEHEGHIDHLVRKALRSGVPSESVYRAASYSVARHYGLREVGAVTPGRWANIIVLDDA
                     PTCAIRTVYLRGTPVQELAFDDSLTRTNLGAHSIRAAAPTEAELTGPKGRVHVIGAIE
                     GKIITTHSVADSTDSGIARLSVLERYGHGHKPANGYVSGFGASLNGAIASSVGHDSHN
                     LIVVGNNTGDMRAALAHLIKTGGGFCVVQNAKVLADLALPVGGLMTPAPTSEIVTKLR
                     SLRTASRTIGCELSEPFLQLAFLSLPVIPKLKLTDRGLIDVEQFKIISVSA"
     gene            1870..>2551
                     /locus_tag="PLH57_07440"
     CDS             1870..>2551
                     /locus_tag="PLH57_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02485.1"
                     /translation="MFKLSRAIILMSVMGGTGVSALAGPFDATNIANAKSSRDCANVL
                     QARSDRVLFGNFRVEHVSLYFNAALEAFGARTGRVMGDPGNAALVLYQMGHDFEFAVR
                     GPAGIEWHPSSVIGRAMVENSANGTPLEKSDILDLKPGFELTVRWLAILHEAKAALVP
                     EYAEQLPNGAVSNGVIYRREGYFGLDVFALFSRSQMEALLWASLSSVATNVQPSLSRL
                     NLLHVEEIL"
BASE COUNT          597 a          618 c          729 g          607 t
ORIGIN      
        1 acgccgttga ggtaaaaaac tacgaagtca cgagcagcgg atttgagagt tttcatgctg
       61 acaccgagat gatcttaaat tgttctacgt cgattaaacc gcgatctgta agttttagct
      121 tcggaattac gggcaaactc aaaaaagcta actgtaaaaa aggttcgctc agttcacagc
      181 cgatcgtgcg acttgctgtt cgcagagatc gaagttttgt aacaatttca ctcgtgggcg
      241 cgggcgtcat cagcccacca acgggcaagg cgagatcggc tagtaccttg gcgttttgaa
      301 cgacacaaaa gccgccgccg gtcttaatca aatgagccag agccgctcgc atatcgcccg
      361 tattgttgcc tacgacaatt aaattgtggc tgtcatggcc gacactagat gcgatggctc
      421 cgtttaaact agcgccaaat cctgagacgt aaccgttggc tggtttgtgc ccgtgtccgt
      481 agcgttccag taccgaaagt cgagcgatgc cggaatcggt agagtcagcc actgagtggg
      541 tcgtgataat tttaccttcg atcgcgccga tgacgtgcac tctccctttg ggaccggtga
      601 gttcagcttc tgtcggagct gcagcacgaa tggaatgtgc gcccaggttg gtgcgcgtga
      661 gcgaatcgtc gaatgcaagc tcttggactg gagtgccgcg taaatacacc gttcgaatgg
      721 cgcaggtcgg tgcgtcatcg agaacaatga tgtttgccca acgcccgggg gtcactgcac
      781 ctacttcgcg cagtccgtaa tggcgagcta cactgtacga agcggcccga taaacagact
      841 cagacggcac tccggaacgc agtgcttttc gaacgagatg atcgatgtga ccttcgtgtt
      901 cgatgtcgag tgggttgcgg tcatcggtgc agaagccgac agtcgccgat gaatacgcgt
      961 ttaagattgg taaaagcgcg tcgaggtctt tggcgacgct gccctcacgg atccacacgg
     1021 ccatcccttt taaaagtttt tcttgagcct cggataactc cgaagattcg tgacagctag
     1081 atatgccaca cgcggcgtag gcggagaggt ctttgccgcg tagttggggt gcgtggccgt
     1141 cgatcggtcg atctgaaaac gcctcaagct tggcgtgcac ttctgggctg cgattcaaaa
     1201 cgccgggcac gttcatgact tcggctaaac ccagcgcccg cgagcgatct ttaagtgcgg
     1261 caagttgcgc cgccgtgatc gtgccgccgc cgttggtttc aagatgagtg gctggtacgc
     1321 acgagctgag catgacgtgg agatctaaat tggttttttc ggcgcagtct aaaaaatagg
     1381 ttagcccctc gacgcccagc acatttgcca actcatgagg gtcgcaaata gccgacgttg
     1441 taccgcgcgc gagcacggct tgttcgaacc gtccgggcac catgcaagaa ctttcaatgt
     1501 gtacgtgagc atcgataaac cctggtacaa ccgcagcacc ttttagatcg atggtgcggt
     1561 gggcggtgta gcctgagtcc aagccgacca cgcgtccgtg gtgcacagcg atgtcggtgt
     1621 gaacccattg acccgagtgg acttcgagta atttagcgtt tttaagcaat agctgagcgg
     1681 gaattatgcc gcgcgcagcc tgcagagttt cggtatagtg accagaagtc aatagcgaca
     1741 gaaccacctc agattacgat tatgaaatta acctaaaact aacatggggg acaggcagtt
     1801 tcacctgttt tagaaaattg acttcctcca gcaatttcac taatgccagg gaacaaaggg
     1861 gtagacggaa tgtttaaact gagtcgcgcg attatactga tgagcgtcat gggtggcacg
     1921 ggtgtgtcgg cgctcgccgg accgtttgat gccacgaata ttgcgaacgc caaaagctcg
     1981 cgcgattgcg ccaatgttct gcaagctcgt agcgatcgtg tgttatttgg aaactttcgg
     2041 gtcgaacatg taagtttata ttttaatgct gctcttgagg cgtttggcgc acgaactgga
     2101 agagtgatgg gggaccccgg gaatgcggca ctcgtactct atcaaatggg acatgatttc
     2161 gaatttgctg tgcgcggacc ggcgggcatt gagtggcacc cttcgagtgt gattggccgt
     2221 gcgatggtcg aaaactctgc gaatggtaca ccgctagaaa aatcagatat tcttgatcta
     2281 aagccggggt tcgaattaac ggtgcggtgg cttgcgattt tacacgaagc aaaggcggca
     2341 ctggtgccgg aatacgcgga gcagcttcca aatggcgcgg tatcaaacgg agtgatttat
     2401 cgtagagaag gttatttcgg gctcgacgtg tttgcgctct ttagtagatc tcaaatggag
     2461 gcgttgcttt gggcaagtct atcgagcgtc gccacaaatg tgcaaccctc tttgagtcgg
     2521 ctaaacctgc ttcatgtcga ggaaattttg c
//
LOCUS       DAOMXO010000334         2538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_23509_length_2538_cov_4.598872, whole genome shotgun sequence.
ACCESSION   DAOMXO010000334 DAOMXO010000000
VERSION     DAOMXO010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23509_length_2538_cov_4.598872"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PLH57_07445"
     CDS             <1..1178
                     /locus_tag="PLH57_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRK02486.1"
                     /translation="AREALSKVSGLAGLSGVGIQLGLKNSQLTVITPIDDTPAYEAGI
                     KAGDRIVKINGESTKGLGLEEAVAKMRGKKGSDIALTIWRKGLKEPKEYKITRAEIKI
                     KTVKSEVLEKDYLYARLTNFNERSSHDLRTAIENFEKSNRLRGLVLDLRNNPGGLLDQ
                     AVDVTSLFVEDGAVVTMAGKDKSKGETKYVKKGFARKDFAVAVLVNGASASASEIVAG
                     ALQDHKRAVVLGQPTFGKGSVQNVVEIAPDVGLKLTIARYYTPNGRSIQMKGIHPDVL
                     LEEFDQKTLAAAKKAVDYVREKDLKQAMNADGEDEVIEALVMEEKAEKEAKDKEVVFD
                     AADRPVKAKDDYQVREALNYIKSARALIASGVSMGEPEVATALSKGAKTSEENPARQ"
     gene            1182..2414
                     /gene="xseA"
                     /locus_tag="PLH57_07450"
     CDS             1182..2414
                     /gene="xseA"
                     /locus_tag="PLH57_07450"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRK02487.1"
                     /translation="MQTDFDFSRKNITSKKKSETGALSVTEITTQVKFLIEGELPASW
                     VRGEVSGMRPSYSGHVYFTLKDSQSALSCALFAGAARKVKVPLKDGDEVMVFGKISVY
                     APRGTYQLIIEKVEAVGAGALALEFEMLKNRLAAEGLFDSARKRQLPEHPRRVAIVTS
                     PTGAALQDFLNVLGRRNSSISVLVAPVVVQGDGAPAQVVQALETINVGQLADLIVIAR
                     GGGSLEDLWCFNDERIVRAVASSSIPVVSAIGHETDFTLCDAVADLRAPTPSAAAELI
                     SRSGQVLLESLIDQERRMRLLVNARISERRERLLFLQQRLVSPQARLAELRKKIFDTF
                     FQVENLAQRRHLAAVGKLESFSGRLEALSPLKVLGRGYTLVEDRVTGDLLKSVNEAKG
                     GRKVWLRFKDGKTEAQIT"
BASE COUNT          664 a          557 c          718 g          599 t
ORIGIN      
        1 aggcgcgcga ggcgctttcg aaggtgagtg ggctggcggg gttgagcggg gtgggtatcc
       61 aactcggttt aaaaaattcc caactcacgg tgataactcc gatcgacgat acgcccgctt
      121 acgaagccgg tatcaaggcc ggtgatcgaa ttgtaaaaat aaatggggag tctaccaagg
      181 gtcttggtct ggaagaagcg gtagccaaga tgcgcggaaa aaaaggcagc gacatcgcgc
      241 tcactatttg gcgcaaaggt ttgaaagaac ccaaggaata caaaattact cgagcagaga
      301 ttaaaatcaa aacggtcaaa tctgaagtgt tggaaaaaga ctatctgtat gcgcgtttga
      361 cgaactttaa cgagcgatct tcccatgatt tacgaaccgc catcgaaaat ttcgaaaaaa
      421 gtaatcgtct gcgcgggtta gtgctcgatc tacgtaataa tcccggcggc ctattggacc
      481 aggcggtcga cgttaccagt ttgtttgtag aagacggcgc tgtagtgacg atggccggca
      541 aagataaaag taagggtgag acaaaatacg taaaaaaggg ttttgcccgt aaagactttg
      601 cagtcgcggt gcttgtgaat ggcgccagtg cgtcggctag cgagatcgtc gctggggccc
      661 ttcaagacca taaacgtgca gtagttcttg gacagcctac tttcggaaag ggttcagttc
      721 aaaacgtcgt agaaatcgct cccgacgtgg gtttaaagct gactattgct aggtactaca
      781 cgcccaatgg tcgctccatt cagatgaagg ggatacatcc cgacgtctta ctcgaagagt
      841 tcgatcaaaa aactttggcg gcagcaaaaa aggctgtgga ttacgtgcga gaaaaagatc
      901 tcaagcaagc catgaatgcc gacggtgaag acgaagtgat cgaagctcta gtgatggaag
      961 aaaaagctga aaaagaagcc aaagataaag aggttgtttt cgatgcagcc gatcgcccag
     1021 taaaagccaa agatgattat caagttcggg aggcgctcaa ctacattaaa agtgcccgcg
     1081 ccctaatagc ttcgggcgtg tcgatgggtg aaccagaagt tgcaacagcg ttgtctaagg
     1141 gtgccaagac atcagaagaa aaccctgcta gacaatagag ggtgcagaca gattttgatt
     1201 tttctcgtaa aaacataaca tccaaaaaaa aatccgagac cggtgcgctc tcggtgaccg
     1261 aaattacgac tcaggttaaa tttttaatcg aaggggagct gcctgcatct tgggtgcgtg
     1321 gtgaagtttc cggaatgcgc ccctcctatt cgggacatgt ctactttaca ttgaaagatt
     1381 cgcagagtgc gctgtcttgt gcgctgttcg ccggcgctgc gagaaaagta aaagttccgc
     1441 ttaaagatgg cgatgaagtc atggtgttcg ggaaaatttc ggtttacgcg ccgcgcggta
     1501 cctaccagct catcattgaa aaggtagaag ccgtaggcgc cggggctctt gcactagagt
     1561 tcgagatgct aaaaaatcgc ctcgctgcgg aagggttgtt tgatagcgcg cgaaaacgcc
     1621 aacttcccga acatccgcga cgggtggcga tagtgacatc tccgacggga gccgcattac
     1681 aggatttttt aaacgtatta gggcgtcgca actctagcat ttcggtgctg gtagctccgg
     1741 ttgttgttca gggggacggc gcccccgctc aggtggtgca agcgctcgaa acaataaacg
     1801 taggtcaatt ggcagattta atcgtcattg ctcgcggggg tggatcccta gaggatcttt
     1861 ggtgtttcaa tgacgaacga attgtacggg ccgtggcctc cagttcaata ccggtcgtgt
     1921 cggctatcgg tcacgagacc gattttactc tttgtgatgc cgtggccgac ttgcgcgcac
     1981 cgaccccatc tgctgcggcg gaactgatat cgcgcagtgg tcaagtgttg ctggagtcgc
     2041 tcattgatca ggaacgacgc atgcgactgc tggtgaatgc acgaatttcg gagcgacgtg
     2101 aacggttgct gtttttgcaa caacggttgg taagccccca ggcgcgattg gccgaattgc
     2161 ggaagaaaat ttttgacact ttttttcagg tggaaaatct tgcccagcgc cgacacctag
     2221 cagcggttgg gaagttagag tccttttcag gtcgtcttga ggccctttct cctttaaaag
     2281 ttctgggacg gggttatact ttagtagagg accgtgtgac gggcgatttg ctcaaatcgg
     2341 tcaacgaggc caagggtggc cggaaagtgt ggctgcgttt caaggatgga aaaaccgaag
     2401 cccaaattac ttgatatcac tcgccttctt tttattgcgg taagtttttt ttccgcaatt
     2461 tttgcacagg cgcagatcaa agtagaaatt gcgagcggcg ctcccgttga gggcggcgtg
     2521 ttttttgtaa acgtgcaa
//
LOCUS       DAOMXO010000335         2527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_23672_length_2527_cov_11.148058, whole genome shotgun
            sequence.
ACCESSION   DAOMXO010000335 DAOMXO010000000
VERSION     DAOMXO010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23672_length_2527_cov_11.148058"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PLH57_07455"
     CDS             complement(<1..995)
                     /locus_tag="PLH57_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02488.1"
                     /translation="MYGDFIIQNPQLRSKEGKADPISYSLTSEQQQRIDSYLKSRSQE
                     VVAAVKNLEFVGTISNGRVSTIFVSRAFQQDEGLKLRQQEWGWNALYGKPIHLGEPGN
                     KVLLGQALARTLGCRPKEKQKVLTPSGGFREGFREFNCDTEDILLSVTTATGQVNSLD
                     FKVSGITDAGYRDIDARYVMADFKDAQTLVNTDRVGYYTLLLEDGISQNKFIRDFEEI
                     VTTKDPAIKIQRWQDHAVGDLYNKTMNLLSVFRNFVIIVLVAISGLSIATTFSKNVKE
                     RTREIGLLLSLGFYRRSVLNIFLYESMILGLLGVGIGLMAGGAFSIILNASQIYY"
     gene            1138..2373
                     /locus_tag="PLH57_07460"
     CDS             1138..2373
                     /locus_tag="PLH57_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004434354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HRK02489.1"
                     /translation="MKTSSDLHNHYSLLLGLLPPWLVTNVDLKVDQRFVEIDVQWPAD
                     HQVECPECQRSCAIKDHREERRWRHLDTMQFQTIIKSRVPRSECPDHGVKTIAVPWAG
                     PNSRFTLLFERFAIDVMIAAKSIKAAAGLLGLSWDQVHHIQARAVERGLLRRKLDDIK
                     HVGIDEKSFLKGHKYASLMVDIDGARILDVVQDRTLEAAEQLWQKLPGEVREGIDAVA
                     MDMWDAFITSTKKHAPQADIVHDKFHISKYLGEAVDKVRRAEHKAFMKDGDETLKGAK
                     YLFLKNPENMNGIEKDRFNCLRIDNLKAGRAWSIKETFSEFWTYSYQASAVKFFRRWY
                     WWATHSRLKPMIEVAKLMKRHLPNILTYLRHQITNAMMEGFNSKIQSIKANARGYRNF
                     ANYRIAILFYCGKLELHPL"
     gene            complement(2330..>2527)
                     /locus_tag="PLH57_07465"
     CDS             complement(2330..>2527)
                     /locus_tag="PLH57_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02490.1"
                     /translation="DAFNPNWLKEAIDTSSKPVFIFAHTSLRDKERYSGDTAQTFSDI
                     GSSDFLVPREGEVRACRNRIV"
BASE COUNT          713 a          554 c          588 g          672 t
ORIGIN      
        1 ttgtagtata tttggcttgc atttaaaata atcgaaaatg ctccgccggc catcaaaccg
       61 ataccaacac ctaaaagacc taagatcata ctttcatata gaaaaatatt taaaacgcta
      121 cgtcgataaa accctaaact tagtagtaag ccaatttcac gagtacgctc ttttacgttt
      181 tttgaaaatg tagtggcgat ggataatccc gaaatggcga caagtacaat aatcacgaag
      241 ttgcgaaaga cgctcagtaa attcatcgtc ttgttgtaga gatctccgac tgcatgatct
      301 tgccagcgtt gtatcttaat tgcggggtcc ttagtcgtta cgatctcttc gaaatcacga
      361 ataaatttgt tttgtgatat gccatcttca agtaacaaag tatagtaccc cacgcgatcc
      421 gtattcacta atgtctgggc atctttaaaa tcggccataa cgtagcgagc atcgatatct
      481 cggtaaccgg catctgtgat accagatact ttgaagtcta aggaatttac ttggccagtg
      541 gctgtggtta cggaaagtaa tatgtcttcc gtatcgcaat taaattcgcg aaagccttca
      601 cggaaaccac cagatggcgt tagcaccttt tgtttttctt taggtcgaca tccaagcgtg
      661 cgtgctagcg cttgtcctag caaaactttg tttccgggtt cacccaaatg gatgggtttt
      721 ccgtacagag cattccatcc ccattcttgt tgtcttagtt ttaagccttc atcttgttga
      781 aaagcacgag agacaaaaat ggttgaaaca cgcccgttac taatagttcc gacaaattca
      841 agattcttta ccgcggctac gacctcttgg gacctggatt ttaggtacga atctatgcgt
      901 tgttgctgtt ccgaagttag cgaatatgaa atggggtcgg cctttccttc tttgcttcga
      961 agttgaggat tctgaataat aaagtcacca tacatctgtc gatgacggta cgaatttaaa
     1021 tataatctag aaacactttc aatgtaacca tcaaaaatta ggggtattcc gggttttctg
     1081 tgggaagcag agcgtagctg gttgaagata gcctaaattt tgggctttga ttttaggatg
     1141 aaaacttcat ccgatttgca caatcactat agtttattac tgggcctttt gccaccgtgg
     1201 ctcgtcacca atgtcgactt gaaagttgat cagcggttcg tcgagatcga cgtccaatgg
     1261 ccggcagacc atcaagtgga gtgtcctgag tgtcagcggt catgcgcaat caaagaccac
     1321 cgcgaagagc gtaggtggcg tcatctggac acaatgcaat tccagacaat catcaaatca
     1381 cgtgtgccac gcagtgaatg ccccgatcac ggagtgaaaa ctattgccgt accgtgggcg
     1441 ggtccgaatt cgcgttttac actgctcttt gaacggttcg caattgatgt gatgatcgca
     1501 gctaagagca ttaaagcggc agcaggcctg ttggggttgt cgtgggatca agttcatcac
     1561 attcaagcac gggctgtaga gcgcggcctc ttaagacgca aacttgacga catcaagcac
     1621 gttggaatcg atgaaaaaag ttttttgaaa ggtcacaagt acgcatcatt gatggtcgac
     1681 attgatggcg cacgcatctt ggatgtggtt caggaccgca cgctggaagc tgcagaacaa
     1741 ctctggcaaa agctccccgg ggaagtgcgc gaaggaatcg acgctgttgc catggacatg
     1801 tgggatgcat tcatcacatc gaccaaaaaa catgctccgc aggcggacat cgtgcacgac
     1861 aagttccata tctcgaaata cttgggcgag gctgtcgaca aagttcggcg tgctgaacac
     1921 aaggcattta tgaaagatgg cgatgagacg ctcaagggcg caaagtactt gttcctgaaa
     1981 aatccagaga atatgaatgg catagaaaag gaccgattta attgtttgcg aatagacaat
     2041 ctcaaggcag gtcgtgcgtg gtcaatcaaa gaaacgttct cggagttttg gacctactcg
     2101 tatcaagcat cggcagtgaa attctttagg cgctggtact ggtgggcgac acactcaaga
     2161 ctgaagccga tgattgaggt tgcgaaacta atgaaacgcc atcttccaaa cattttgact
     2221 tacctgcgcc accagatcac gaacgcgatg atggaggggt tcaactcaaa aatccaatcg
     2281 atcaaagcca atgctcgtgg ctaccgaaat tttgccaact accgtatcgc tatactattc
     2341 tattgcggca agctcgaact tcaccctctc taggcacaag aaagtcggaa gaaccaatat
     2401 cagaaaaagt ttgcgcagta tcacccgagt agcgttcttt atctcgtaag ctcgtatggg
     2461 caaaaataaa tactggttta gaggatgtgt cgatggcttc ttttaaccag tttggattga
     2521 atgcatc
//
LOCUS       DAOMXO010000336         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_23724_length_2524_cov_3.818145, whole genome shotgun sequence.
ACCESSION   DAOMXO010000336 DAOMXO010000000
VERSION     DAOMXO010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23724_length_2524_cov_3.818145"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2524)
                     /locus_tag="PLH57_07470"
     CDS             complement(<1..>2524)
                     /locus_tag="PLH57_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK02491.1"
                     /translation="LKRNSRPAWGIGRRDVCGAVLGGVYFFQTLSLALALLLGVASYQ
                     AHAKLLEKSTFEITAPEVQAAAEDPARFYVKTQNGSWAQDKFIYSPDTGLLLRLAENR
                     EVTAVHSFGVIPGLTENDLEILSFSSLIPFESEEPSRVIVRHGVWMSIVDAQEDRLIM
                     GNIGPVEPGVGGKWFNREVVVGGKVRYYTFVARTALRNHPQTPENAPDRELMNRLVMV
                     DLETQSVGVLPLRSDIEPIEMRIVGTDAGAIRLVGRYHSPQPGSRNPFQKAEMIVDLK
                     ELNVTQAEGVSFFANRPISNMKVLRAVRGMFSNDQLAERLMLPGITSEQLMSQGNPND
                     TAAKTAALSLVQQKIADAKLIEGAVRDGLRNIHGQKDAIQALVALAQGRATAKKAQVS
                     VAGGTTGTGKTSGVAAFAKALFPQKTQPVFEISFSENTEEKFLQTTLFGSAPGYIGMD
                     TPSGLMVWLLANPDGGVLLFDEIEKAPPEAMKILMSFLDRQEVKITPQLLKFMIASYQ
                     EMKLEVEHWPLAIREATSNGTKLDVGVTLRLTPDHFIFLNTNAGASLYNGSGISNVAS
                     QRLETEQQLRAANARFNGEVIKAELRERGYEDEILNRVSTYIPFKVIMPEDHKYIVRD
                     RLAEIEAYFKKEYFIDLTYSPEAYEYLVQATYSPLDGARFVENQLRKWVTSNLTEALF
                     QTETLRAGQAVRWTLDPSDGRRHNQLVLVHEENGKLNELGRYNVGKAPPAAPTWILER
                     AETRLLPMLVDRIRGHQHEKEQLANRTISNLKKAVKAPSKAGGVVSFEYSDGPPGTGK
                     TEIAKVSAEAIFEDPRAMIVVEMNNINSMDDFYRMLVQPLKAA"
BASE COUNT          604 a          654 c          581 g          685 t
ORIGIN      
        1 cagcggcttt gagcggctgt acaagcatgc gataaaaatc gtccattgaa ttaatattat
       61 tcatttcgac cacaatcatg gcccgtggat cttcgaaaat tgcttccgcg gatacctttg
      121 caatttctgt ttttccggta cccggaggtc catcgctata ctcaaaagaa accacaccac
      181 ctgctttaga gggtgccttc accgcttttt ttagattcga aatggtgcga ttagcgagtt
      241 gttctttttc gtgctgatgc ccgcgaattc gatcgactaa catgggcaat aagcgggttt
      301 ctgctcgttc caaaatccat gttggcgctg cgggcggggc tttacccacg ttgtaacgcc
      361 cgagttcgtt gagtttacca ttttcctcgt ggaccagaac caactgattg tgtcgacgcc
      421 catcgcttgg atctaatgtc catcgcacag cctgacctgc tcgaagtgtt tccgtttgga
      481 aaagggcctc ggttaggttg gaagttaccc acttgcgaag ctgattctcc acgaatcgag
      541 cgccatcaag cgggctatag gtcgcctgaa ctagatattc ataggcctca ggactgtaag
      601 tgagatctat aaaatactct ttcttgaagt aagcttcaat ttcagccagt cgatctcgca
      661 caatatactt gtgatcttca ggcatgatta ccttgaacgg aatgtaggtc gaaaccctat
      721 ttaggatttc gtcctcgtaa cctctttcgc gcaattctgc ttttatgact tcgccattaa
      781 agcgagcgtt ggcggcccgc aattgctgct cagtctcgag acgctgggaa gccacgtttg
      841 aaattcccga accattatag agtgaggcgc cagcattggt atttagaaaa ataaaatgat
      901 ccggagttaa ccgtaaagtt accccgacat ccaatttagt gccgttgctg gtggcctctc
      961 gaatcgcaag cggccagtgc tcaacttcga gcttcatttc ctggtaactc gcaatcatga
     1021 acttcaagag ctgcggcgtg atttttactt cctgacggtc cagaaaagac attagaattt
     1081 tcattgcttc gggaggtgct ttttcaattt cgtcgaaaag caaaactccg ccgtccggat
     1141 ttgctagcag ccaaaccatt aagcccgaag gtgtgtccat tcctatgtaa ccgggtgccg
     1201 aaccaaagag agtggtttgc agaaacttct cttcggtgtt ttccgaaaaa ctaatttcga
     1261 aaactggttg agttttctga ggaaatagtg cttttgcgaa tgcggcaaca ccgctagttt
     1321 tgcccgttcc ggtggttccg cccgctaccg acacttgagc tttcttggcc gtcgctctac
     1381 cttgagccaa ggcaacgagt gcctgaatcg catctttttg accatgaatg ttacggagac
     1441 cgtcgcgaac tgcaccttcg attaattttg catctgcgat tttttgctgc accaatgaca
     1501 gtgcggctgt tttggccgcg gtgtcattcg gattcccctg actcatcagt tgttcggaag
     1561 taataccggg taacatgagt cgttcggcca actgatcgtt ggaaaacatt ccccgcacgg
     1621 cgcgtaatac tttcatgtta gagatcgggc gatttgcaaa aaaactcact ccttcggcct
     1681 gggtgacgtt gagttcttta agatcaacta tcatttccgc tttttgaaat ggatttctag
     1741 agccaggttg cggcgagtga taacgaccaa caagtctaat tgcacccgcg tcggtaccaa
     1801 cgatccgcat ttcgattggt tcgatatcag acctcaaagg caatactccg acgctctggg
     1861 tctcgagatc gaccatcact aatcgattca ttagctcgcg atctggcgca ttttccggag
     1921 tttgcgggtg atttcgaagc gcagtgcgcg ccacaaacgt gtaataacga acctttccgc
     1981 caaccaccac ttcgcggtta aaccattttc cgcctactcc gggttccacc ggtccgatgt
     2041 tgcccatgat caatcgatct tcttgggcgt cgacaatcga catccacacg ccgtgtcgca
     2101 cgattactcg cgacggctct tctgattcga agggaattaa cgacgagaac gacagaattt
     2161 ctaaatcatt ttctgtaaga ccgggaatga ctccaaatga atgaaccgcg gtaacttccc
     2221 gattctccgc caagcgcaag agcaacccag tgtcgggcga atatatgaat ttatcctgcg
     2281 cccaacttcc gttttgagtc tttacgtaaa accgcgccgg atcttcggcg gcggcctgaa
     2341 cctctggtgc cgtaatttca aaagtcgatt tttctagtaa cttcgcgtga gcttggtatg
     2401 aagccacacc caaaagtaac gccagtgcta aactcagcgt ttgaaaaaaa tagactccac
     2461 ctagaactgc cccacacaca tcgcggcggc ctatccccca ggccggacgc gaattccttt
     2521 tgag
//
LOCUS       DAOMXO010000337         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_23736_length_2523_cov_5.587925, whole genome shotgun sequence.
ACCESSION   DAOMXO010000337 DAOMXO010000000
VERSION     DAOMXO010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23736_length_2523_cov_5.587925"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="PLH57_07475"
     CDS             complement(<1..1209)
                     /locus_tag="PLH57_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRK02492.1"
                     /translation="MSITKKLQKVRPSLTQWILIALVGGFLVGVFAPSLVPWLKPFRG
                     LFLNGIKCIVAPLIFATVVTGISSAGSFRQLGSMGVRAFIYFEVVTTAALVVGLVAVN
                     LFRPGDGLTLGHTLNDKVQAAAQTKMSFGSFVEHLLPHNFADAVVRGDVLQIVIFSTL
                     FGFATLSLGSRAKPVIDFSEALSHVMFKFTGYIMTLAPLGVGAAMAASVAEHGWQVIV
                     PLLKLVGTLYIALIAFLLLVMLPIALAFKVPVIAFMKELKGSILLAFATTSSESAYPS
                     ALESLERLGVPRRIASFVLPMGYSFNLDGSTLYLALASVFVAQAAGVELTLWQQLTIM
                     ATLMLTTKGVAAVPRASLVVLSGTLTMFGLPLEGIALILAVDEFMDMARTAVNLLGNC
                     LATAVITRWEG"
     gene            1331..1600
                     /gene="rpsT"
                     /locus_tag="PLH57_07480"
     CDS             1331..1600
                     /gene="rpsT"
                     /locus_tag="PLH57_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRK02493.1"
                     /translation="MANVKSAIKRARQNIKRRDRNQALRTTARSAAKKALDGIKAAST
                     KEAAIIALREGERALQKAVSKGVWPKSRASRKVSRLAAAVTGRFA"
     gene            complement(1612..>2523)
                     /gene="holA"
                     /locus_tag="PLH57_07485"
     CDS             complement(1612..>2523)
                     /gene="holA"
                     /locus_tag="PLH57_07485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRK02494.1"
                     /translation="QGKAEPIYYLYGDEPFKLREFTDKFQDAFLGEQADRSFSVDRLD
                     GSVCSVSEIIDTAATTGFLFSASNESAGRRLVFVRQAQSLKNTEKLLPWCEEQRSVGG
                     GSSVVLLLGDSLDGRKKLHQWFRAQGLAVEFKKAADRELVQWAQYLAKKRGITLSGEA
                     VESLAFLCDGSLNRLDQEIEKAWLYRGGSAGIELAARDVAAVASQDVSHEMADFVAAV
                     LEGKRVRALLSCGKVVRSAEDALGFVGFCVWAIKNPERFRSTGSLLRRGQVQTALLGL
                     ERLDRKLKSSGAEPQSLLNEFILQVTE"
BASE COUNT          684 a          746 c          588 g          505 t
ORIGIN      
        1 cccttcccag cgagtaatca ccgcggtggc aaggcaattt ccaagtaaat tcacggctgt
       61 tcgagccata tccatgaatt cgtcgaccgc taaaatgagg gcgatccctt caaggggcaa
      121 accaaacatg gtcagggtac ctgagagcac cacgagcgaa gcccgaggaa cggccgcaac
      181 gcccttggtc gtcaacatga gggtcgccat gatcgtcaac tgctgccaga gtgtgagctc
      241 cacaccggcg gcctgagcca cgaacaccga ggccaaggcc agatagagcg tcgagccatc
      301 gagattaaaa ctatacccca tggggagcac gaaactggca atgcgccggg gcacgcccaa
      361 acgttcgaga gactccaggg ccgacggata agccgactcg ctcgatgtcg tggcaaacgc
      421 cagaagaata gaccctttta attctttcat aaacgcaatg accggaacct taaacgcgag
      481 cgcaatggga agcatgacta gcaacaaaaa ggcgatcaga gcgatataga gagttcccac
      541 gagctttaac agaggcacga tcacttgcca tccgtgttcg gctacgcttg ccgccatggc
      601 ggcaccaacc cctaggggcg caagtgtcat gatgtagcca gtgaatttaa acatcacgtg
      661 actgagagcc tcggaaaaat cgatcaccgg ttttgcgcga ctccccaggg aaagcgttgc
      721 aaatccaaag agcgtcgaaa atatcacaat ctgcagtaca tcgcctcgaa cgacggcgtc
      781 ggcgaaatta tggggcaata ggtgctccac gaaacttccg aatgacatct ttgtctgagc
      841 ggccgcctgc accttgtcgt tcaaagtgtg gcccagagtt aatccgtcgc ccggtcggaa
      901 aagattcacc gccactaagc ccaccactaa agccgccgtg gtgaccactt caaaatagat
      961 aaacgcccgc acacccatgc tgccgagctg ccgaaaactc ccggccgagc tgatcccagt
     1021 cacgacggta gcgaagatca aaggagctac aatgcatttg atcccattaa gaaatagacc
     1081 tcggaatggc ttaagccacg gcaccaaaga gggagcaaac actccaacca agaagccgcc
     1141 aactaacgcg atcaggatcc actgcgtgag tgacggccta actttttgaa gtttcttggt
     1201 aatcgacacg acgcccccct gaccggccca aaatcagacg ttctacccta cctacaactt
     1261 catgttgaaa agaaaggtaa ttttagataa acgctaggtt cgtttttaac ttattagagg
     1321 agttcactct gtggcaaacg taaaatccgc aatcaaacgc gcgcgtcaga atattaagcg
     1381 ccgcgaccgc aaccaagcgc ttcgcacgac tgctagatcg gctgcaaaaa aagcactcga
     1441 cggcattaaa gctgctagca ccaaagaagc tgctattatc gcgcttcgcg aaggcgaaag
     1501 agctttgcaa aaggcagtaa gcaagggtgt gtggccaaag agccgcgcta gtcgcaaagt
     1561 ttcgcgcctg gctgcagccg ttactggtcg tttcgcttaa gcagcagcga tctattctgt
     1621 tacttgcagg ataaattcat ttaacaagct ctggggttcc gccccggagc tttttaattt
     1681 gcggtccaac cgctctagtc ccaataaagc tgtttgcacc tggccccgcc ttaataacga
     1741 acccgtgctt cgaaacctct cgggattctt gattgcccac acacaaaacc ccacgaaccc
     1801 aagcgcatct tccgcgctcc gtacaacttt gccgcaggac agcagcgcac gcactctttt
     1861 accttcgagt acagcggcca caaaatcggc catttcatgg gagacatcct gactcgcaac
     1921 tgcggcaacg tctctcgccg caagctctat tcccgcactc ccaccgcggt acagccatgc
     1981 cttttcaatc tcttgatcca gacggttcaa agaaccgtcg cacaaaaaag ccagcgactc
     2041 gacggcctcg ccagagagcg taatgcctcg tttttttgcc agatactgcg cccactgcac
     2101 aagctcgcga tccgcagctt ttttgaattc aaccgccaga ccctgagccc gaaaccactg
     2161 gtgaagtttt tttcgtccat ctagcgaatc tccaagaagc aacaccacgc tggatccgcc
     2221 gcccacggac ctttgttctt cacaccatgg cagtaacttc tcggtatttt taagtgactg
     2281 cgcctgacgc acgaacacca gccgtcgccc cgcactttcg ttgctagcgg aaaacaaaaa
     2341 cccggtagtc gctgccgtat cgataatttc actaactgaa cagacagacc cgtctaaccg
     2401 atctaccgaa aaggaccgat cagcctgctc tcctaaaaaa gcgtcttgaa atttatcggt
     2461 aaattcacgc agcttaaaag gctcgtcgcc ataaagataa taaatcggct ccgccttgcc
     2521 ctg
//
LOCUS       DAOMXO010000338         2520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_23779_length_2520_cov_4.853144, whole genome shotgun sequence.
ACCESSION   DAOMXO010000338 DAOMXO010000000
VERSION     DAOMXO010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23779_length_2520_cov_4.853144"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLH57_07490"
     CDS             <1..342
                     /locus_tag="PLH57_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02495.1"
                     /translation="TASQAATPSPLKVREDVFKKSIRLSPAAHILKLQWNVHRSVAPK
                     RIKTYLAVVRNQVCVETLELQPQEHRLLESIKNPVSTESFLNFVAKQTTATKLKKWIT
                     RWSQNNLIQFE"
     gene            362..829
                     /locus_tag="PLH57_07495"
     CDS             362..829
                     /locus_tag="PLH57_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02496.1"
                     /translation="MTNSPEYWRKCTSCKSLIGFGQKYWICSVSTCNRVRTGLVFCKV
                     DCFDAHVPVLNHKDAGAIEKRAPSAAEWMREQSESESVRPQPSASTAHAETKRDILVV
                     VSKTKDYVRAKAGLNTSENVMEVLSDKIRAILDDGIRRAIQDGRKTLMDRDFK"
     gene            complement(830..>2520)
                     /gene="speA"
                     /locus_tag="PLH57_07500"
     CDS             complement(830..>2520)
                     /gene="speA"
                     /locus_tag="PLH57_07500"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HRK02497.1"
                     /translation="HRVITLSEAFRKSIEEAGYKGRYQGVYPVKVNQLREVVEEILDA
                     GAPYNMGLEAGSKAELYASLAMNTNPEALVLVNGYKDSGIIKMALLGQRIGKKVIIIV
                     EKLSELGHILRVGREIGVRPQIGLRCKLHARGSGKWESSSGESAKFGLTTPEILHAIQ
                     ILKEENMVDCVKLVHFHIGSQITDIRSIKDATKECTRVYAKLRKMGVDIEYLDVGGGL
                     GVDYDGSRTSFSSSINYSLQEYVSDIVYTIQEVCAGEEVPEPNIVSESGRALVAYHSV
                     LVLNVFGNIEVGKSPYVLEESPEEADVVREMRYTLANLSPKNLLEAYHDVLQRKEEAA
                     SMFKLGFLTLEDKAKVENLFWRVCLNLSKLIVGQKYVPEELQNMSKHLADQYLCNFSL
                     FQSAPDHWAIQHLFPIVPLHRLNEEPTRQSTLVDITCDSDGKVDKFIDLRDVRETLPL
                     HPLNAEPYYLGMFLMGAYQDIMGDIHNLFGRVNEVHVFEDAEEPGGYYIEETIQGNTV
                     GGILSTIQYNTGEMIRQVKSAIDAKVKAGALKPREGVELLDHYERGMTEYTYID"
BASE COUNT          695 a          646 c          529 g          650 t
ORIGIN      
        1 actgcttctc aagcggccac cccttcccca ttaaaagtac gggaagacgt tttcaaaaaa
       61 tcgattcggc tttcgcctgc cgctcacatt ttaaaattgc agtggaacgt gcaccgtagc
      121 gttgctccta agcgaatcaa aacttatttg gcagttgttc gaaatcaagt gtgcgtcgaa
      181 acacttgaat tacagccaca ggaacaccga ctactcgaaa gcattaaaaa ccctgtttcg
      241 actgaatctt ttttaaactt cgttgcaaaa cagacgaccg ccactaaact caaaaagtgg
      301 attacaaggt ggtctcaaaa caacctcatt caattcgaat gattaggcat atgagcaaaa
      361 tatgacaaac agccctgagt attggcgaaa atgtaccagt tgtaagtctc ttatcgggtt
      421 cggacaaaaa tactggattt gcagcgtctc aacctgcaac cgcgttcgca ctggactggt
      481 gttctgtaaa gtagactgtt tcgatgccca tgtgccggta ctaaaccaca aagatgctgg
      541 agcgattgaa aaacgagccc caagcgccgc tgaatggatg cgcgaacaga gcgagtccga
      601 aagcgttcga ccccagccat cggcttccac tgcccacgcc gaaaccaaaa gagatattct
      661 tgtagtagtt tcaaaaacca aggactacgt tcgcgcaaaa gccgggctca atacttcgga
      721 aaacgtgatg gaagtgctct ccgataaaat tcgtgcaatc ctcgacgacg gcattcgccg
      781 ggccattcaa gatgggcgca aaactttgat ggaccgagat tttaagtaat taatcgatgt
      841 aggtgtattc ggtcatccca cgttcatagt ggtccaataa ctcgacaccc tcacgaggct
      901 ttaaagctcc ggccttgact ttcgcatcaa tagccgactt tacctgccga atcatttcac
      961 ctgtgttgta ttgaatggtt gaaaggatcc cgcctaccgt attcccttga atggtctctt
     1021 cgatataata accaccaggc tcttcggcat cttcaaatac gtgcacttca ttgacgcgtc
     1081 caaaaaggtt gtgaatatcg cccattatat cttgataagc gcccatcaaa aacatgccta
     1141 aataataagg ttcggcattg agtgggtgca aaggtagagt ttcacgcacg tctcgcaaat
     1201 caataaactt atccacctta ccgtcggaat cacaagtaat gtcgaccaga gtagattgcc
     1261 gcgttggttc ttcgttcaag cggtgcagcg ggacaatagg gaacaaatgt tgaattgccc
     1321 agtgatccgg cgcgctttga aatagcgaaa aattacagag atactgatcg gccaaatgct
     1381 tagacatgtt ttgtaattct tcgggtacat atttttgacc aacgattaat ttcgacaggt
     1441 tcaagcaaac tcgccagaaa agattttcga ctttcgcttt gtcttcgaga gtcaaaaatc
     1501 cgagtttgaa catactggcc gcttcttctt tgcgttgaag aacgtcgtga tacgcttcca
     1561 acaagttttt cggcgataaa tttgccaaag tatatcgcat ctctcgcaca acatcggctt
     1621 cttcgggaga ttcttctaaa acatacgggg atttccccac ttcaatgttc ccgaaaacat
     1681 tcaaaacgag cactgaatga taagccacca acgctcgtcc cgactcgctt acgatgttcg
     1741 gttccggaac ctcttcaccc gcacaaactt cctgaatagt ataaacaata tcgcttacgt
     1801 attcttggag agagtaattg atcgagctcg aaaaagacgt gcgactacca tcgtaatcca
     1861 cgcccaagcc gccgccgaca tctaaatatt caatgtcgac acccatctta cgtaacttgg
     1921 catatacccg cgtacattct tttgtagcgt ctttgatcga gcggatgtcc gtgatttggg
     1981 agccaatgtg aaagtgcacc agcttcacac aatcgaccat gttctcttct tttaaaattt
     2041 gaatggcatg gagaatttca ggagtcgtga ggccaaactt ggcggactcc ccgctcgagc
     2101 tttcccactt gccactaccc cgagcgtgca atttacaacg cagtcctatt tgcggacgaa
     2161 cgccaatctc gcgacccaca cgcaaaatgt ggcccagctc actgagcttt tcgacgataa
     2221 tgataacttt ttttccaatt cgctggccca acaaggccat cttgataatt ccagaatctt
     2281 tataaccgtt gaccaatacc aacgcttccg ggttggtgtt catggcaaga ctggcataga
     2341 gctcggcctt cgaacctgct tcaaggccca tattataagg ggcacctgcg tcgagtatct
     2401 cttcaaccac ttcgcgcaat tggttcacct taacagggta aaccccttgg taacggccct
     2461 tgtaccctgc ctcttcgatg gatttccgaa acgcctcgct cagtgtgatt actcggtgac
//
LOCUS       DAOMXO010000339         2518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_23801_length_2518_cov_5.115713, whole genome shotgun sequence.
ACCESSION   DAOMXO010000339 DAOMXO010000000
VERSION     DAOMXO010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23801_length_2518_cov_5.115713"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLH57_07505"
     CDS             <1..728
                     /locus_tag="PLH57_07505"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRK02498.1"
                     /translation="CGCSMGITAENLGAKYGITRGQVDEFALLSQTRAKAAAEAKRFD
                     TEIEPYAIKDKKGNVVEIKADEHPKPATTIESLTKLKSVFKENGLVTPGTASGIVDGA
                     AMFVVSTENFAKRKNLKPLGRLVAYGIAGVDPSIMGIGPAPAVRQALKRAGMSLSQID
                     LVEINEAFGAQYLAVEKELELDRERTNVNGGAIAIGHPLAASGSRITMHLLYELQRRG
                     KRYGLGAACIGGGQGIALVVEAF"
     gene            750..1820
                     /gene="ftsY"
                     /locus_tag="PLH57_07510"
     CDS             750..1820
                     /gene="ftsY"
                     /locus_tag="PLH57_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRK02499.1"
                     /translation="MLFSDPVVLVSTLSVGVGISILAFIFFFRKKSVKTQTSVVSESS
                     KLVPPAKDQPTSASRVHSGMSRTREYFRGAFDRLFSGSQGSEFFSNLEEVLLSADVGI
                     EYAEKIIQKLKEQYGLRTPSREELKSSLHSVLVSGLPKGMPLLDASSDAHPHVVMIVG
                     VNGAGKTTTIAKLCALYRAKGRRVLVGAADTFRAAAVEQLKTWVERTGAEGVFQKEGT
                     DPSAVAFDTVAAAVSRKVDLCLVDTAGRLHTKVNLMEELKKMKRVIAKADSTAPHDVW
                     LVVDGSTGQNALRQAKEFHAALSLTGVVVTKLDGSSKGGAVLALSTELGIPIMFIGVG
                     ESPEDLVPFDANPFVDAILTGL"
     gene            1858..2151
                     /locus_tag="PLH57_07515"
     CDS             1858..2151
                     /locus_tag="PLH57_07515"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRK02500.1"
                     /translation="MESEKKYVTVEILDQKFTFASDAPTERVERVAEFVNASLRDALA
                     RSKKPTPYHAAILVALNSAEKYFELLEKHDDFKSSVTNRSKKILGMLESVKGV"
     gene            2158..>2518
                     /locus_tag="PLH57_07520"
     CDS             2158..>2518
                     /locus_tag="PLH57_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02501.1"
                     /translation="MDLHAPKKNLRVHYRAARAELSKSDHAQFSQKICDRVWYSFFHY
                     DALKDPLLRKRAVALYFPIDHEVNISFLSHKLFSEEIPIALPDFSDPTRPFKLVRDFS
                     EVRMDGKIPLPPADAKPV"
BASE COUNT          662 a          579 c          643 g          634 t
ORIGIN      
        1 attgtggttg ctctatgggc atcacagcgg aaaacctagg tgcgaaatat ggtataacgc
       61 ggggacaagt cgacgagttc gccctacttt ctcagacccg ggcaaaagca gctgccgagg
      121 ctaaacgttt cgataccgaa atcgagccct acgcaattaa agataaaaag ggaaatgtag
      181 ttgaaataaa agcagacgag cacccgaaac ctgcgacgac cattgaatca ctcacgaaac
      241 tgaaatcagt attcaaggaa aacggattag tcactccggg tacggcgagc ggtatcgtgg
      301 acggtgctgc catgtttgtg gtttcgaccg aaaatttcgc taagagaaaa aatcttaagc
      361 ccctcggacg gttagtggct tacggaatcg caggagtgga ccctagtatt atgggtatcg
      421 gtccagcccc tgccgtgcgc caagcgctta agcgggcggg aatgtcactt tctcaaatag
      481 atttggtcga aattaacgaa gcatttggag cgcagtatct tgcggtcgaa aaggagctcg
      541 agcttgatcg agagcgcact aatgtaaacg gcggagcgat tgccattggt caccctttgg
      601 cggctagcgg ttcgagaatc acaatgcatt tactttatga gttgcagcgc cggggcaaac
      661 gctatgggct tggggccgct tgcattgggg gcggtcaagg gattgctcta gttgttgaag
      721 ccttttaatt aagttttagg atttatctca tgttgttttc cgatccagtc gtattggtct
      781 caacccttag cgtaggggtt ggaatttcaa tcttggcgtt catttttttc tttcgaaaaa
      841 aatccgttaa gactcagacc tcggtagtgt ccgaatcttc gaaactcgtg ccgcctgcca
      901 aagaccagcc tacttcggcc tcgcgggttc actcgggaat gagtcgtacg cgagaatatt
      961 ttcgaggcgc atttgatcga ctcttttccg gctctcaagg gtcagaattt ttttccaact
     1021 tagaagaggt acttctctcg gccgacgttg gaatagaata tgctgaaaag ataatccaaa
     1081 agttaaaaga acaatacggt cttcgaactc ctagtcgtga agagttaaaa agttcgttgc
     1141 attcggtatt ggtgagtgga cttcccaaag gaatgccact tctagacgca tcctcagacg
     1201 cccaccccca cgtcgtcatg attgtcggag tcaacggagc cggcaaaact accactatcg
     1261 ctaagttatg tgcgctttat cgggcaaagg ggcgccgtgt gctcgttgga gcagcagata
     1321 ctttccgcgc agcggccgtc gagcagctta aaacctgggt ggaacgcacg ggagcggagg
     1381 gcgtttttca gaaagaggga acggatccat ccgctgtggc cttcgatacg gtggctgcag
     1441 ccgtgagtcg caaagttgat ctgtgtttgg ttgatactgc ggggcgtttg catacaaagg
     1501 tcaatctcat ggaagaatta aaaaaaatga agcgcgttat cgctaaagcc gactcgacgg
     1561 ctccgcacga tgtatggttg gtagttgatg gttccactgg gcaaaatgca ttgagacaag
     1621 cgaaagaatt ccacgcggcg ctttctctca ctggagtggt ggtcacaaaa ctcgatgggt
     1681 cctccaaagg cggtgcggtt ttggctctga gcacagaact cggaattccg atcatgttta
     1741 ttggtgttgg cgagtccccg gaggatctag tcccgtttga cgcaaaccct tttgtcgacg
     1801 caattttgac tggactatag gtcttattaa agtttcaata agcgaactgg gggtcgtatg
     1861 gaatcagaaa aaaaatacgt cactgtagaa attttggatc aaaaatttac cttcgcctcc
     1921 gatgctccga ctgagcgcgt ggaacgggta gcggaattcg taaacgcatc tttgcgagac
     1981 gcgttagcaa gatcgaagaa gccgacgccc tatcacgcgg ccatcctggt tgcgcttaat
     2041 tcggcagaaa aatatttcga attactcgaa aagcacgacg actttaaaag ctccgtgacg
     2101 aaccgttcca aaaaaattct cggtatgctc gaatcagtaa aaggcgtcta gtttctggtg
     2161 gatctgcacg caccaaaaaa aaatctacgc gtccactacc gagcggcccg cgcagaactt
     2221 tcaaaatccg atcatgcaca gttttctcag aaaatttgcg accgcgtttg gtattcgttt
     2281 ttccattatg atgcccttaa agacccactt ttaagaaaac gcgcagtagc gctttatttt
     2341 ccgatagatc acgaagtaaa tatttcattt ttgagtcata aactttttag cgaagaaata
     2401 cccatcgcgc ttccagattt ttcggaccct acgcggccat tcaaacttgt gcgagatttc
     2461 tcagaagtcc gcatggatgg gaaaattcca ttgcccccgg ctgacgctaa accagtac
//
LOCUS       DAOMXO010000340         2513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_23862_length_2513_cov_4.688365, whole genome shotgun sequence.
ACCESSION   DAOMXO010000340 DAOMXO010000000
VERSION     DAOMXO010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23862_length_2513_cov_4.688365"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="PLH57_07525"
     CDS             complement(<1..1279)
                     /locus_tag="PLH57_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02502.1"
                     /translation="MICDPRGSLWRKWDLQVHPPGCKLNDAYDTNDGAERLDKFCSIL
                     EASDVQVFGITDYFSVDAFRAFIGRFNERYPKSEKVFFFNLELRLNETVNKETEEVNV
                     HLLFNPNSLSSVDKFLSRLKVEKTGPRSVAVTCAELATSDDFESATVSRQSIAEAFEE
                     TFGKRAIRQDHFLVITAANNDGIRAVRGKKRKENICDEIDKFSDAFFGGQKNVDHFLK
                     LDRFEDNALNSPRKPTFASSDSHSFGDIEAFLGKRVAEEIEAGGRKQRAITKDVTWLK
                     ADTTYDGLRQTLYEPESGERVYIGPLKPDEKDAYKVIRKLTFGDSADFPAEIVFNDNL
                     CSIIGSRSSGKSALLAYIAHSVDPDGVANIIDGPGEGEDYHWHKIKLEYSIQWADGST
                     NDESPGNIVYIPQNYLFDMTKNADIIKAKIEPIL"
     gene            complement(1298..1573)
                     /locus_tag="PLH57_07530"
     CDS             complement(1298..1573)
                     /locus_tag="PLH57_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02503.1"
                     /translation="MNIPKDVIEGPWYAFALQDFSCWICDRVPTIPFPAIPMIDDEGQ
                     ATTWAQWWGDLNQWWHVTICSPVSYWANCNIRTAANHESTSYNWPSF"
     gene            complement(1711..>2513)
                     /locus_tag="PLH57_07535"
     CDS             complement(1711..>2513)
                     /locus_tag="PLH57_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRK02504.1"
                     /translation="KGRITAGPQRHGYTFRRLFTCAKCGNTLTGEGHKGHVYYRCHTR
                     GCNQASVREEAIDAAIEAAFIPLQFSNRERLYLQGKLRTLKTNWQTTTERELRSLELR
                     HNEVRERLSRLTDAVIDKLINKCEYEERKAELLMEQQDIAERKEAMTASTGSLGETVT
                     KFFELAESLYLGYKMGIPAEKRDLIKMATSNRTVNGKDVVVKPCFPFDLIAKRSGVLK
                     GAPSRARLRTLDEMLAKVVGHFESNPESASRLRGHGDFRDVEGQDCAA"
BASE COUNT          601 a          639 c          566 g          707 t
ORIGIN      
        1 aaagtatagg ctcgatcttc gccttgatta tatcggcatt cttggtcatg tcgaacagat
       61 agttttgagg tatgtagaca atattgccgg gactctcgtc gttggtactc ccatcagccc
      121 attggatcga atactccagt ttaattttgt gccagtggta gtcctcgcct tcaccaggac
      181 catcgataat gttcgcaact ccatccggat caactgagtg agcaatgtac gcgagtaggg
      241 ccgatttacc tgatgagcga ctgccaatga tcgaacataa attgtcatta aatacgattt
      301 ctgcaggaaa gtcggcggag tcgccgaatg ttaattttcg gattaccttg tatgcgtctt
      361 tctcatcagg tttgagtgga ccaatgtaaa cccgttctcc tgactccggt tcatatagag
      421 tttgtcggag cccgtcatac gtggtgtcag ctttaagcca agtcacgtct tttgtaatcg
      481 cgcgttgctt tctaccgcct gcttcgattt cttctgccac cctttttccc aggaaggcct
      541 cgatatctcc aaaagaatgg ctgtcgctgc tcgcaaacgt tggctttctc ggcgaattaa
      601 gagcgttgtc ttcaaatctg tctagcttta ggaaatggtc gacatttttc tgaccaccaa
      661 agaatgcgtc actaaacttg tcgatttcgt cgcaaatatt ctcttttctc ttctttccgc
      721 gtaccgctcg aattccgtca ttgtttgccg ctgttattac caaaaaatgg tcttgtctga
      781 tagccctttt tccgaaagtt tcctcgaatg cctcggctat tgattgcctg ctcactgtcg
      841 cagactcgaa atcatccgaa gtagccagtt cagcacaagt aacagcgacg gatcttggac
      901 cggttttttc aactttcaat ctgcttaaaa acttatccac actcgacagg gaattcgggt
      961 tgaacagcag gtgaacatta acttcctcag tttctttgtt caccgtctca tttagcctta
     1021 gttcgaggtt aaagaagaag actttttccg atttgggata acgctcattg aaacgaccta
     1081 taaacgcccg gaatgcatct accgaaaagt agtcggtgat accgaatacc tgaacgtcgg
     1141 aagcttctaa tatactgcaa aatttgtcga gtcgctctgc gccatcattg gtgtcatacg
     1201 catcattaag tttacatcca ggaggatgaa cctgcaggtc ccattttcgc caaagtgaac
     1261 cacgtggatc gcaaatcatg gaattaatct ttccggctta aaatgagggc cagttgtagc
     1321 ttgttgattc atgatttgca gccgttcgta tattgcaatt ggcccaataa gacactggcg
     1381 aacaaatcgt cacatgccac cactgattaa gatcacccca ccattgagcc caagtcgttg
     1441 cctggccttc gtcgtcaatc atcggaattg ctggaaatgg gatggtcggt actcggtcac
     1501 agatccaaca actaaagtct tgaagagcga aggcatacca tggtccctca atcacgtctt
     1561 tgggaatatt catcacccgt ctcattagcc acaagcgtaa caggtaaggg aaacagaggc
     1621 aagataggca ggacgtgggg actttaggac tttgtagcgg gtatggtgca ccaattcgcc
     1681 taacgcggaa aattcaagct attctcctgc tcaagccgca caatcttggc cttcaacgtc
     1741 cctaaagtcc ccatgtcccc gtagacgtga ggccgactcc gggttagact caaagtgacc
     1801 cacaaccttc gcgagcatct cgtccaaagt tcgaagtctg gctcgtgacg gggcaccctt
     1861 aaggacaccc gaccgttttg ctatcaaatc aaacggaaaa cacggcttaa ccacaacatc
     1921 tttcccgttt accgttcggt tcgaagtggc cattttgatt aaatcgcgct tttctgcggg
     1981 aattcccatt ttatagccta aataaaggct ttctgcgagt tcgaagaatt tcgttaccgt
     2041 ctcacctaga gagccggttg acgcagtcat ggcctccttc cgttccgcaa tatcctgctg
     2101 ttccatcaat agttcggctt tgcgttcttc gtactcgcac ttattgatca gcttgtcgat
     2161 taccgcatcc gttagccggg aaagccgctc gcggacttca ttgtgccgca gttcgagcga
     2221 acgcaattca cgttcggtgg tcgtttgcca gttggtcttg agcgttctca atttgccttg
     2281 tagatacagg cgctcacggt tgctgaactg gagcgggatg aatgccgctt caatggcagc
     2341 gtcgattgct tcttctcgca cagaggcctg gttgcagccc cgagtatggc agcgataata
     2401 aacgtgccct ttgtggcctt cgccagttaa cgtgttacca cattttgcgc aagtaaataa
     2461 gcgccgaaac gtataaccat ggcgctgggg acccgcagtg attcttccct tta
//
LOCUS       DAOMXO010000341         2508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_23927_length_2508_cov_6.319201, whole genome shotgun sequence.
ACCESSION   DAOMXO010000341 DAOMXO010000000
VERSION     DAOMXO010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23927_length_2508_cov_6.319201"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /gene="carB"
                     /locus_tag="PLH57_07540"
     CDS             <1..692
                     /gene="carB"
                     /locus_tag="PLH57_07540"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HRK02505.1"
                     /translation="ASRTVPFISKAVGVPLAKIATQVMLGRTLKELGINDDLDLQLKT
                     FNIKMPVFPFHKFPGSDVLLGPEMKSTGEVMGRARDFSLAYAKALCGSGAKVPTHGTA
                     FLSVADIDKPALLEIAKKLQNMGFRLEATSGTRDFLEKNGLRSEMVNKYGNGSPNCVE
                     GIAANKYQFVINTAYNEQSVLDSYTIRRTALEKKVPYCTLITTARAFLRAIESAQNNP
                     IATSVAPITEP"
     gene            703..993
                     /locus_tag="PLH57_07545"
     CDS             703..993
                     /locus_tag="PLH57_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02506.1"
                     /translation="MSLTCYSCSKTLDPLDFNRADRCPHCGRDTRVCRNCIHYDTSKY
                     NECAETTADRVVDKEKSNFCDHFKPGQPTSKGAGLSAAEKAKLAAEALFKKK"
     gene            1116..>2508
                     /locus_tag="PLH57_07550"
     CDS             1116..>2508
                     /locus_tag="PLH57_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02507.1"
                     /translation="MSFLSGSYAFALPRSMRDVPPTPGALREECRFILEVSSAIEVEK
                     KYTTHEVLQLLNKEEKSIRHEMSWLEAEIGKPDNQRKLKDPRFFSRFRSPEKNPVTIL
                     FREQMISLKTALKLVEANFAIVRSLNEVEKRSSNVEINQRFYNASNASPIDAVLRQWI
                     LSEVANQTLQLVPKARILHANVARGEFDRAIAFSAMIQSSVRTLDTLTNNHASSSVIE
                     TEQTALASLLLRKGQIRIREAIKLKFLNSNHNESVPLDFELAVDLLTASVAQTKSSID
                     AVIQRFGQVSALFPREARLDNTAYAYLTHYSLAHSISAEDAVRQYNQSYKRVTASSTG
                     LERNVKTAVGTQENLALMELYADAAENPHRDTAVPYAFAAARKITDYNDTAAVFLAKA
                     ILVKGTRASESLYRNIQRQLGATSLATNPIALAYLTLISAQLNVPDATVAQTATNFGS
                     KLQFDDPFFNRSPV"
BASE COUNT          712 a          625 c          536 g          635 t
ORIGIN      
        1 gagcaagccg caccgttccg ttcattagta aggccgtggg cgtgccgctt gcaaaaattg
       61 ccactcaagt aatgctcgga cgaactctaa aagaacttgg aataaatgat gatctcgatt
      121 tgcagctcaa aacatttaat atcaaaatgc cagtttttcc atttcataaa tttccgggat
      181 ccgatgtcct tctaggaccc gaaatgaaat caaccggaga ggttatgggg cgagctcggg
      241 acttttcgct ggcctacgca aaggcacttt gcggttctgg cgccaaggtt cccacccacg
      301 gaacagcttt cctttcggtt gcagatatag acaaaccggc gcttctcgaa atcgcaaaaa
      361 aattacagaa tatgggattt cgccttgagg cgacctcggg aactcgcgat tttctagaaa
      421 aaaatggtct gcgctcagaa atggtcaata agtacggaaa tggatctccc aactgtgttg
      481 aaggtatcgc ggcaaataaa taccaattcg tcattaacac ggcatataac gagcagtctg
      541 tgctcgatag ttacaccatt cgtcgaacgg ctttagaaaa aaaggtccct tactgtactt
      601 tgataaccac tgctcgggcg tttttgcgag cgatcgaaag cgcgcagaat aatccgatag
      661 caacatctgt tgcgccaatc acggagcctt agatttagtt ttatgtcact tacctgttat
      721 tcctgcagca agacgctcga ccctctcgat tttaaccgtg ccgaccggtg cccccattgc
      781 ggacgagaca cccgggtttg tcgcaattgc attcattacg atacgagcaa atacaatgaa
      841 tgcgccgaaa ccactgccga tcgcgtggta gacaaagaga agtccaattt ttgtgaccac
      901 tttaaaccag ggcaacccac ttcaaaaggc gcaggcctct cggctgccga aaaagctaaa
      961 ctggccgcag aggctctgtt taaaaagaaa taagaccctg gaattattaa gatttcctac
     1021 actctaaata cccgtttaat taagtatagt atagtgatat attgctgcgc atgcttgggc
     1081 atcaataccg cattgtaagg gactccctgc taatcgtgtc attcctttct ggatcatacg
     1141 cttttgcgct tcctcgctcg atgcgagacg ttccgcctac gccgggagcg ctacgagagg
     1201 agtgtcgatt catcctcgaa gtgagtagcg ctattgaagt agaaaaaaaa tataccaccc
     1261 atgaagtttt gcagctctta aacaaagaag agaaaagtat tcgacacgaa atgtcctggc
     1321 tggaagccga aattggcaaa cccgacaatc aacgaaagtt aaaagatccg cgatttttta
     1381 gtcggtttcg atctccagaa aagaatcccg taactattct ttttcgagaa caaatgatca
     1441 gtctgaaaac tgctctcaaa ttagttgaag ctaatttcgc tatagttcgc agtttaaatg
     1501 aagtggaaaa acgctctagc aacgtcgaaa tcaatcaacg cttctacaat gcctctaatg
     1561 cctctccaat tgatgcagtt ctgcgccagt ggatactctc ggaagttgct aatcaaactc
     1621 ttcaacttgt tccgaaagct cgtattttgc atgcaaacgt ggctcgtggt gaattcgacc
     1681 gggcaattgc tttttcagcc atgattcaat cgtcggttcg aacgttagat actcttacca
     1741 ataatcacgc gtccagctct gtgatcgaaa ccgaacaaac agctctggcc agtctgcttc
     1801 ttagaaaagg gcaaattcga atacgcgaag ctataaaatt aaaatttctg aattctaacc
     1861 acaatgaatc cgtgccgctc gactttgaac ttgcggtcga tttactaacg gcatcggttg
     1921 cgcaaacgaa atctagcatt gatgcagtaa tccaacgctt cggacaggtt tcagcacttt
     1981 tcccacgaga ggcgcggctc gacaacaccg cctacgctta tctaacccac tactcgctcg
     2041 cgcattccat tagcgctgaa gatgctgttc gtcagtacaa ccaaagctat aaacgggtga
     2101 ccgcaagcag caccggttta gaaagaaatg ttaaaacagc cgtgggcact caagaaaacc
     2161 tcgcgctcat ggagctctac gctgacgcgg ctgaaaatcc tcaccgcgac actgcagtac
     2221 cctatgcttt cgcggctgcc cgaaaaatta ctgattacaa cgacacagca gcggtttttt
     2281 tagcgaaagc tatcttggtt aaaggcacac gtgccagcga atcgttgtat cgaaatattc
     2341 aacgccaatt gggggcaact tcactggcta ccaaccctat agctttagcc tatttaacgc
     2401 taatcagcgc ccaacttaat gtgcctgacg ctaccgtggc ccagactgca acaaattttg
     2461 gttccaagct ccaattcgat gatccctttt ttaatcgttc tcctgtac
//
LOCUS       DAOMXO010000342         2503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_24001_length_2503_cov_4.795343, whole genome shotgun sequence.
ACCESSION   DAOMXO010000342 DAOMXO010000000
VERSION     DAOMXO010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24001_length_2503_cov_4.795343"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /gene="thyA"
                     /locus_tag="PLH57_07555"
     CDS             complement(<1..465)
                     /gene="thyA"
                     /locus_tag="PLH57_07555"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRK02508.1"
                     /translation="MRQYLDLMKHVLEKGAKKEDRTGTGTLSVFGYQMRFDLSEGFPV
                     VTTKKLHLKSIIYELLWFLKGDTNIEFLKQNGVSIWDEWADENGNLGPVYGHQWRSWA
                     GADGKSVDQISEVIAQIRKNPDSRRLIVSAWNVGELHKMATKKAWQGGKAILC"
     gene            556..2148
                     /locus_tag="PLH57_07560"
     CDS             556..2148
                     /locus_tag="PLH57_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRK02509.1"
                     /translation="MGKTTLRTAFSRYVVALLCVGLGTIASAKANDLVDEWQVEYSEE
                     QKARDYADQLSLRNGSDWATAFVTALAQNKKALAQALFERRKNFGDLSRVVLNKSGEC
                     LASALIGKSPRFLEILLNEGLDPDCEFVPNLARPLWLSLVTKKVAEAKTLLSHGANTD
                     FYFRSPTQTFWRQLMQEKYAGFAKKLFSTKQLIEQKDPSGTCFFSAAVVWGAEQLVAH
                     ALKKKASIAAPVGGAQDYFGLALMNSQFGAAKLIAQNTTAKSILVNFRDKTDSTYLHW
                     CADLGESSLCETLAEIGLSLTSIDSAGRTPRDRALIAGNQKIARLLVPEGSNNPEKDF
                     LLTQTLNKNCKSIESFFVDRNDPKGILEINDWDKNGNTLLHLASAKGSSKCVRTFLKY
                     GASARLPNRLSETALHLSVVNDHLNSTRLLLAAGADPSDINVQGESPFHLAARHGLVP
                     QIEALYGAGAYKKYIVYLTNSEGRTALHLATIHKQPLAMRALLGKNRAHVTAQDDYGF
                     TAYDYAKKSQDSLAIAILEAAQ"
     gene            2269..2343
                     /locus_tag="PLH57_07565"
     tRNA            2269..2343
                     /locus_tag="PLH57_07565"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2301..2303,aa:Gln,seq:ttg)
BASE COUNT          641 a          616 c          575 g          671 t
ORIGIN      
        1 gcataaaatg gctttaccgc cctgccacgc tttttttgta gccattttat gcaactctcc
       61 cacgttccag gcgctcacta ttaatcttct ggagtcaggg tttttgcgta tctgggcgat
      121 aacttcggaa atttgatcta ccgattttcc gtcagccccg gcccatgatc gccattggtg
      181 accgtaaacc ggacccaagt taccattttc gtcggcccat tcgtcccaaa tcgaaacacc
      241 attttgttta agaaattcaa tatttgtgtc gccttttagg aaccaaagaa gctcgtaaat
      301 gatagacttc aaatgaagtt tcttagtcgt gacgaccgga aacccttcgc tcaaatcgaa
      361 tcgcatttga taaccgaaaa cactgagtgt gcctgtgccc gttcggtctt cttttttggc
      421 gcccttttcg agcacgtgtt tcatgagatc cagatattgg cgcatgaaag ctcctcttca
      481 ctagaagtca tacccccatt gtagggtccg gtccatcatg ttatcgggtc cctggactat
      541 aggggatctt aaaacatggg aaaaacaact ctgcgcaccg cgttttcgcg gtatgtggtg
      601 gcactgttgt gcgtgggact ggggacaatc gcatcggcta aagccaacga tttagtcgac
      661 gagtggcagg tcgaatattc ggaagaacag aaagctcgcg attatgccga tcagcttagc
      721 ctcagaaacg gtagcgattg ggcgacggct tttgtgacag ccctcgctca aaacaaaaag
      781 gcactggcac aggcactatt cgagcgccga aaaaattttg gcgacctctc gcgagttgtt
      841 ctcaacaaaa gtggtgaatg tcttgccagc gcactaattg gtaaatcacc tagatttctg
      901 gaaattctct taaacgaagg tctggacccc gactgcgaat ttgtcccgaa tctcgcccgc
      961 ccgctatggc ttagcttggt gaccaaaaaa gtagctgagg ctaaaactct gctctcacat
     1021 ggcgcaaaca ctgatttcta ctttcgttcg ccgactcaaa cgttttggcg acaactgatg
     1081 caggaaaagt acgcagggtt cgcaaaaaaa cttttctcca ctaagcagct catcgaacaa
     1141 aaagatccaa gtggtacctg ttttttttcc gcagcggtgg tttggggagc ggaacaatta
     1201 gtggctcatg ctcttaagaa aaaagcctcg attgccgccc ccgtcggtgg tgcgcaagat
     1261 tactttgggc tggcactaat gaacagtcag tttggcgctg ctaaattgat cgctcagaat
     1321 acgactgcca aaagtattct ggttaatttt agggataaaa ccgactcaac ttatttacac
     1381 tggtgcgccg acttgggtga gtcttcgctt tgtgaaacac tggctgaaat tggtctttcg
     1441 ctcacctcga tagattcggc aggtcgcacc ccacgagacc gggcactaat tgcgggcaat
     1501 caaaagatag ctcgtttatt agtacctgaa ggttctaaca atcctgaaaa agattttctt
     1561 ttaactcaga ccctgaataa aaactgtaaa tccatcgagt ctttttttgt ggaccgcaat
     1621 gatcccaaag gaattttgga aattaatgac tgggataaaa acggtaatac tctgcttcat
     1681 cttgcgagtg caaagggttc ttcgaagtgc gtaagaactt ttttgaaata tggagcttct
     1741 gctcggttgc ccaaccgcct ctcggagacc gccctgcact tgagcgtagt taacgaccac
     1801 ctgaactcta cccgtttgtt attagccgcc ggagcggacc cctctgacat taacgtccag
     1861 ggagaatcac cgttccacct cgcggcaaga cacggtctag taccccaaat cgaagccctc
     1921 tatggcgcgg gcgcctacaa aaagtacatc gtttatttga ctaacagtga ggggcgtacc
     1981 gccctgcatt tagctacaat tcacaagcaa ccactcgcca tgcgcgcatt gcttggtaag
     2041 aatcgcgccc atgtaaccgc acaagacgac tacggtttca cggcttatga ttacgcaaaa
     2101 aagagtcaag actctttggc aattgcaatt ctagaagccg cccaatgagc aactagggtt
     2161 actcttctta attgtggaga gccattgcaa cctctaagga gtggtgctaa ttatctggcg
     2221 tctcagagat cgtttgtccg gaaattatat tgagtttccg cgtaggcttg gggtgtcgac
     2281 aagcggtaag tctctggatt ttgattccag cattcctagg ttcgaatcct agcaccccag
     2341 ccaaaataaa aggggccgag tcgcgcaagc gtttcggccc cttttatttt gtaatcatcg
     2401 attactagct tcgcgctcgc caggtgccac attcgcgcag tgaaggggca catttaaaag
     2461 tacttttttg ccactttttg aaattttctc gaatttagaa ttt
//
LOCUS       DAOMXO010000343         2494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_24130_length_2494_cov_3.517425, whole genome shotgun sequence.
ACCESSION   DAOMXO010000343 DAOMXO010000000
VERSION     DAOMXO010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24130_length_2494_cov_3.517425"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..747)
                     /locus_tag="PLH57_07570"
     CDS             complement(52..747)
                     /locus_tag="PLH57_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02510.1"
                     /translation="MSCLAMDPRIIALEKSRYRPPRPPKKKPPNGFIPFLLILLLLAA
                     AALHARQSHAGVFDLPSFLEAGQWSFGVEPEVIISNGTGAGINFKPRFGVNNNLNWQA
                     IIGTGTGSRKFRIGAIADFEWFPDHEKQPGIATPFMVKYSRVDDDGVLAIGVMPLLYK
                     SFDGEGGRYTPFISFPIGWDMRSAKVTGFTQVALGSLFKLPTMEKFKYVLEAGFNIHQ
                     SHSYISGGFTYYP"
     gene            886..962
                     /locus_tag="PLH57_07575"
     tRNA            886..962
                     /locus_tag="PLH57_07575"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:His,seq:gtg)
     gene            1086..1889
                     /locus_tag="PLH57_07580"
     CDS             1086..1889
                     /locus_tag="PLH57_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK02511.1"
                     /translation="MIFFENVSKLYRSAKDSNPDSQDSSHHSAAVNNLSLKLEPGKTH
                     VFIGPSGCGKSTLLRLIAGLEAPTSGTVKVKGQIPTHIPPKVYARNIGFVIQEGGLFP
                     HLSVYENLSIGPFGTQLTRTEQSERINARIEELLQLVHLEKLFLKRFPHQLSGGQRQR
                     VALARALMHDPGYLLLDEPLGALDPVVRSDLQQSLKEIFQSLKKTVVLVTHDLMEAAT
                     LGNTLYLMNKGSVVQSGTFKDIHLNPHDDFVRTFITAQKPSQEFLDLWN"
     gene            1880..>2494
                     /locus_tag="PLH57_07585"
     CDS             1880..>2494
                     /locus_tag="PLH57_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRK02512.1"
                     /translation="MELNFKGSTLYAALLFGLTFFFGFTPLYANSFTVGSKVFTENYI
                     LAEIIAQTMESEIKNRQRSESVDRKIGLGGTGIVYQALLAREIDVYPEYTGTISEEIL
                     KQPQLKNINDLKTALETKGLTISLPLGFNNTYAIATSRRFAEEHDLKNISDLKKVPRA
                     RFGISYEFAQRKDGYYAMLSHYKMKDFFVRRMEHSLAYEALAANK"
BASE COUNT          699 a          606 c          560 g          629 t
ORIGIN      
        1 aaagaacctg taaccggcgc atgagtggag tgctggtgca tatttttcta ttcaggggta
       61 gtaagtaaag ccgcccgaaa tgtagctatg tgattggtga atgttgaatc cggcttcgag
      121 cacgtattta aatttctcca tcgtaggtag tttgaacaaa cttccaagtg ccacctgggt
      181 aaaacccgta accttggcgc tacgcatgtc ccacccgata ggaaacgaaa taaacggcgt
      241 ataccgacca ccttcgccgt caaacgactt ataaaggagc ggcatgacgc caatggcaag
      301 cacgccgtcg tcgtcgacac gactgtactt caccataaaa ggagtggcga tgccgggttg
      361 tttttcgtga tccggaaacc attcgaagtc agcgatagct ccaatacgaa atttgcggga
      421 cccggtgccc gtgccaatga tggcttgcca attgaggtta ttgttcacgc caaagcgcgg
      481 tttgaagttg atcccggcac cggtgccatt gctaatgata acttctggtt ccacaccgaa
      541 ggaccactgc cccgcttcta ggaaactagg aaggtcaaac acgcctgcgt gggactggcg
      601 ggcatgaagg gccgccgccg ccaagagcag caaaatcaat aagaatggta taaatccatt
      661 tggcggcttc tttttaggag gccgcggcgg gcgatagcga gacttttcaa gcgcaataat
      721 gcgcgggtcc atagctaaac agctcatgca ataaatgtcc ttaactaatt gatttctgtg
      781 ttctttatat gtcgaccgga gcccaaaata cactcctttt ttggctcatt tgagggttga
      841 ttcctcgccc cgaacccact ataagaattt ttcgaacttt ggacggtggt cgtagctcag
      901 ttggttagag cgtcgggttg tggtcccgaa ggtcgggggt tcgagacccc tcgatcaccc
      961 catttcaaaa ataaaaaggc cctaagcgaa aacgacgggt cttttttctc ttacatatca
     1021 acaagtgcgg cctgcgacga aagccgcgaa acgatttaaa aacctaaatt tttcgccaga
     1081 aacctatgat tttttttgaa aacgtatcga aattatatcg ttcagcgaaa gattctaacc
     1141 ctgattctca agattcgtct caccacagcg cagctgtaaa caatctttca ctaaagctcg
     1201 aaccagggaa aacccatgta tttattgggc ctagtggctg tggcaagtcg actttattga
     1261 gactcatcgc cggactcgag gcgccaacat cgggcaccgt aaaagtaaaa ggccaaattc
     1321 ctacccacat cccgccaaaa gtttatgccc gaaacattgg cttcgtcata caagagggcg
     1381 ggctatttcc gcacctcagt gtttacgaga atctctcgat cgggcctttc ggaacccaat
     1441 taacacgtac cgagcaatcc gaacgcatta acgcacgcat cgaagaactg cttcagctcg
     1501 tgcatctcga aaaacttttt ctaaagaggt tcccgcacca attgagcggc ggccaaagac
     1561 agcgcgtcgc gctagctcgc gcactcatgc acgaccccgg ttatttacta ttagatgaac
     1621 ctcttggtgc tttagacccg gtcgtgagat cagacttgca gcagagtttg aaggaaattt
     1681 ttcagtcgct aaaaaaaaca gtcgtactcg taactcacga cctcatggaa gccgcaactc
     1741 ttggaaatac tctctatttg atgaacaagg gttcggtcgt acagtcggga acatttaaag
     1801 atatccattt aaacccgcac gatgatttcg tgcgaacgtt tatcactgcc cagaaaccgt
     1861 cgcaggagtt tctcgaccta tggaattaaa ttttaaaggc tcgactcttt acgctgcttt
     1921 gctttttggg ttaacatttt tttttggctt cacgcccctt tacgcgaact cattcactgt
     1981 aggttcgaaa gtttttacgg aaaactatat tttagctgaa atcattgctc aaacgatgga
     2041 gtccgagatc aagaacaggc aacgcagtga atcggtcgac agaaaaatcg gactcggcgg
     2101 cacgggaatc gtgtaccagg ctttactcgc ccgcgagatt gacgtttacc ccgaatacac
     2161 cggtacaatt agtgaagaaa ttttaaagca gcctcaatta aaaaacataa atgatctcaa
     2221 gaccgcactc gaaaccaagg gtttaacaat ttcactgcct cttggattca ataatactta
     2281 cgcgatcgca acctctcgca gatttgccga agaacacgat ttaaaaaata tctccgattt
     2341 aaaaaaggta cctcgggccc ggttcggaat tagctatgaa tttgcacaac gcaaagatgg
     2401 ctattacgca atgctttcac attacaaaat gaaagatttt tttgtcagaa gaatggaaca
     2461 ttctctcgct tatgaagcgc ttgctgctaa taaa
//
LOCUS       DAOMXO010000344         2478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_24357_length_2478_cov_8.916220, whole genome shotgun sequence.
ACCESSION   DAOMXO010000344 DAOMXO010000000
VERSION     DAOMXO010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24357_length_2478_cov_8.916220"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLH57_07590"
     CDS             complement(<1..413)
                     /locus_tag="PLH57_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02513.1"
                     /translation="MTMASPEENNPRPRSAVLASIIVVAVFLTVLGSATARYWMELFK
                     KTENPLCANEVVETIVELALQSDYAQLRKWMNETGPEKTKNCIGAVDGRFDRFGQARM
                     ELLKALASMRNEGISGPTRSEWGGALSFAARSILRP"
     gene            complement(417..1418)
                     /locus_tag="PLH57_07595"
     CDS             complement(417..1418)
                     /locus_tag="PLH57_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRK02514.1"
                     /translation="MGTRSRQLWMIFGILFAGMCLVRVLSVSAEEDIEEIYANEHRGS
                     AAYDDEDTSERDEIFESQRAEIRLSRPAEAASRIAVDEEQPLVPGVQELWAREQQTNP
                     NQAVAKLTVPNKNGPGIGRHVEASRGLASLPPNSDGNREASPDYSARDTERHQNEVYS
                     SHQGNSATPLASSAMEPRGLALNVQSIPIDHRAEQPLPDVVNRGGVQEVSVIVSDYGF
                     FPNRVPVIAGVPVKIFLATTTKQTSCFMLDEFGVKKGISPGKVDEVVFVPTGTGRVRF
                     YCPMKSIEGWLVVKEAPSDANRGLAASAISTAELEPIDQPQYNTPKNAGRLRALIED"
     gene            complement(1490..>2478)
                     /locus_tag="PLH57_07600"
     CDS             complement(1490..>2478)
                     /locus_tag="PLH57_07600"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRK02515.1"
                     /translation="VPAEALTAAWMAAVFGDEAGLARHVHSLEGYAEHGPLPDGSHSV
                     GSALSLIETVFGYGGPLSMRAAAERAVATETDGLTPFYAMAHMGFGHAAFISGELNRA
                     VEHLEKVRMSEASPDIIKVMALSLYSLAEGELGRLERSRELAESALEIVESNQLQVMP
                     QAAAAFAALGQVQAAFGKVDDAMDTLEQGLAIRRRYPIGFWGPLHHVMVMARVAVQAE
                     RVPLAQELLSDLAARMSRFPDGMSTMRARQAAIEAALREQLAAQAPEEELTTRELDIL
                     QLLQGSLSIREIGDELRLSPNTVKSHAKALYRKLGAHSRDEAVATARRNQLI"
BASE COUNT          483 a          779 c          697 g          519 t
ORIGIN      
        1 gggcgcaaaa ttgaacgagc tgcaaaggaa agggcgccgc cccattcgga gcgagttggg
       61 ccagagatcc cttcgttgcg catcgacgca agggccttga gaagttccat tcgcgcttgg
      121 ccaaatcgat caaatcgccc gtcgactgcc ccaatgcaat ttttagtctt ttctgggccg
      181 gtctcgttca tccattttct gagttgcgca taatcggact gcagcgcaag ctctacgatg
      241 gtttcgacaa cttcgtttgc gcaaagaggg ttttctgtct ttttaaaaag ctccatccaa
      301 taccgtgcag tggcgctccc aagaacggta agaaaaacag ctacaactat aatcgatgcc
      361 aataccgccg atcgcggacg tgggttgttt tcttcaggag atgccatggt catgaactag
      421 tcctcgatca gagcgcgcaa acgacctgcg ttcttgggtg tattatactg gggttgatct
      481 atcggctcta gctctgctgt actaatcgcg ctcgcagcca agcctcggtt ggcgtcgcta
      541 ggggcctctt tcacgaccaa ccaaccttcg attgatttca ttgggcagta aaaacgcacg
      601 cgacccgtgc cagtaggcac gaacaccact tcatcaactt tcccgggaga aatacctttt
      661 ttcacgccaa attcgtcgag catgaaacat gaagtctgct tggtggtagt agccaaaaat
      721 attttaaccg gtacgcctgc aattactggc acccgatttg gaaaaaaacc gtagtcactt
      781 acgatcacac ttacctcctg cacgccgccg cgattcacga cgtcgggcaa aggttgttct
      841 gctcgatgat caattggaat gctctgtacg tttaaagcga gaccacgcgg ttccatcgca
      901 ctcgatgcaa gtggtgttgc cgagttgccc tgatgactcg agtagacttc attttggtgt
      961 ctttcggtat cacgagctga atagtccggg ctcgcctctc gattgccgtc ggagttcggc
     1021 ggtaaacttg ccagcccacg cgaagcttcg acgtggcgcc caattccggg tccatttttg
     1081 ttgggcactg ttagcttagc cacggcttga ttgggatttg tttgttgttc gcgagcccaa
     1141 agttcttgaa cacctggaac aaggggctgc tcctcatcga ctgcaatacg cgaggcggcc
     1201 tcagccggcc gcgatagccg aatctctgcg cgttgggact cgaaaatctc gtcgcgctcg
     1261 ctggtgtctt catcatcata cgccgccgat ccgcggtgtt cgttggcgta aatttcttca
     1321 atatcttctt cggcggaaac gcttaacacc ctcaccaagc acatgccggc aaagagaatt
     1381 ccgaaaatca tccataattg acgtgatctt gtacccataa cctacttgct cccaagagcc
     1441 tacacccgcc gtctcacccc gggtgatgtc gctacccacc cctgctggtc tagatcagct
     1501 ggttgcgacg cgccgtggcg acggcctcgt cacgcgaatg ggcgcccagc ttgcggtaaa
     1561 gcgccttggc gtggctcttg acggtgttgg gcgagaggcg cagctcgtcg ccgatctcgc
     1621 ggatgctgag tgagccctgc agcagctgaa ggatgtcgag ttcgcgggtg gtgagctcct
     1681 cctcaggagc ctgcgcggcg agctgttcgc gcaacgccgc ctcgatcgcg gcctgccggg
     1741 cccgcatcgt ggacatgcca tccgggaacc gggacatccg agcggccaga tcgctgagca
     1801 actcctgggc cagcggcacc cgctcggcct ggaccgcgac ccgcgccatc accatcacgt
     1861 ggtgaagcgg cccccagaac ccgatgggat agcgccgccg gatcgcgagc ccctgctcga
     1921 gggtgtccat cgcatcgtcg accttgccga acgccgcctg gacctggccc agcgccgcga
     1981 acgccgcagc agcctgcggc atcacctgga gctggttgct ctcgacgatc tccaaggcgc
     2041 tctcagccag ctcccggctg cgctccagcc gcccgagctc gccctcggcc agtgagtaga
     2101 gggacagagc catcaccttg atgatgtcgg gggatgcctc ggacatgcgc accttctcca
     2161 ggtgctcgac cgcccggttg agctcgccgc tgatgaaagc cgcatgcccg aagcccatgt
     2221 gtgccatcgc gtagaacggc gtcagtccat cggtctcggt cgccaccgcg cgctcggctg
     2281 cggcccgcat cgacagcggc ccgccgtacc cgaagacggt ctcgatgagc gacagtgccg
     2341 acccgaccga atgggacccg tccggcagcg gcccgtgctc ggcgtacccc tcgagcgagt
     2401 gcacgtgccg agccaggccc gcctcgtcgc cgaacaccgc cgccatccag gcggccgtga
     2461 gcgcctcggc ggggacga
//
LOCUS       DAOMXO010000345         2464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_24553_length_2464_cov_5.399751, whole genome shotgun sequence.
ACCESSION   DAOMXO010000345 DAOMXO010000000
VERSION     DAOMXO010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24553_length_2464_cov_5.399751"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..669)
                     /locus_tag="PLH57_07605"
     CDS             complement(46..669)
                     /locus_tag="PLH57_07605"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRK02516.1"
                     /translation="MVESSNQGSGRAFQLTSFNSSLILRIMLFLKNAAINTAILATYY
                     ALHSLLASDFLKQRTPVRSETYRKFYILFAILTAGLAYGAWTPLSNHYSLNAIGTLIS
                     LIGLIGLIWTAKHMDLGVFFGLHSEVSAKLTTNGPYRWMRHPTYTFTLCLGVTPWPSR
                     DSMLFVGVTLVYAIVGSSLEEARLIKVFGNSYRSYQKRTSRLIPGII"
     gene            724..1470
                     /locus_tag="PLH57_07610"
     CDS             724..1470
                     /locus_tag="PLH57_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02517.1"
                     /translation="MFDRGDFPMIERDFAHLAERHDHFGEELERDLSVAHDLSVAHDL
                     SAAHDLSVAHDLSVAYDLSVAHDLSAACDLSVAHDLSVAHDLGPVVACVMAGLLAGLG
                     AGPCVDLGDGLAVDLGDGLAVDLGDGLGADHGVDLADCRAQVLDGLALGLLRDLSTRL
                     FHGDRRYELPLGPEVAPRGSCYESHEQCELQRLSRCRPLELLGVQKNRVSYGGDAHVD
                     GLRTSHFCKSFYTLFECGGDPVLGGDEGLH"
     gene            1454..2137
                     /locus_tag="PLH57_07615"
     CDS             1454..2137
                     /locus_tag="PLH57_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02518.1"
                     /translation="MKVCTKEVLIFDKRPVRPVGVVNRSLVAEFKSVLKAGSVPLFKT
                     RQITILECWVVRRNSYEAVGIKERSAEGAIDVVWIRYPAPPEISEVFPVTVVVGDLAP
                     GIERVPYRTIGFMVIKITALKWSPAHRSVVRKPNPKAADDRPSSHAAQKIVIRKVTAV
                     VVVVVVIRRFDIFRFINYVVFRTCVRNSHRDSDRKHREQIDTKKPDAVGARRAMMVWV
                     HSFLLHKNG"
BASE COUNT          589 a          600 c          667 g          608 t
ORIGIN      
        1 aggaaagcga ttttctcgat tctgttcgtt cgctagccgt agggattaaa taattccggg
       61 gatcagccgc gacgtgcgtt tttggtaaga gcggtagcta ttgccaaaaa ctttgattaa
      121 tcgggcttct tccaaagatg atccgacgat tgcataaacc aacgtaacac ccacaaatag
      181 cattgagtcg cggcttggcc atggagtgac tccaagacaa agcgtaaacg tataggttgg
      241 gtgccgcatc caacggtacg gcccgttggt cgtgagtttc gccgacacct cggagtgaag
      301 tccaaaaaaa acgccgagat ccatgtgttt ggcggtccag atcaaaccaa ttagcccaat
      361 caatgaaatt agtgtgccga tggcgttgag agaataatga ttcgaaagag gagtccaggc
      421 cccgtatgcg agcccggcgg ttagaatggc aaatagaatg taaaactttc tataggtctc
      481 ggagcgcacg ggagtgcgtt gttttaaaaa gtcggaggcc agaagcgagt gtaaagcgta
      541 gtaagtcgcg agaatggcag tgttgatggc ggcgttcttg aggaacaaca tgattcgaag
      601 aatgagcgac gagttaaaag acgtcaattg aaatgcccgc ccgctgccct ggttcgatga
      661 ttcgaccatg gcagcgggcg agattacaga ctaaacggcg gagaatttac cgacgatgat
      721 ctcttgtttg accgcggtga tttcccgatg atcgagcggg atttcgccca ccttgccgag
      781 cgccacgatc attttggcga ggagcttgaa cgcgatctct cggttgcaca cgatctctcg
      841 gttgcacacg atctctcggc tgcacacgat ctctcggttg cgcacgatct ctcggttgca
      901 tacgatctct cggttgcaca cgatctctcg gctgcatgcg atctctcggt tgcacacgat
      961 ctctcggttg cacacgatct cgggccggtt gttgcgtgtg tgatggcggg tctactcgcc
     1021 ggtcttggtg ctggtccctg cgttgatctc ggcgatggtc ttgccgttga tctcggcgat
     1081 ggtcttgccg ttgatcttgg cgatggtctt ggcgctgatc acggcgttga tctcgccgat
     1141 tgtcgcgcgc aggttctcga tggtctcgca ctcgggctct tacgcgatct ctccactcgt
     1201 ctctttcacg gcgatcggag gtacgaatta ccactcggtc ccgaggtcgc accacgtggg
     1261 tcgtgttacg agtcccacga acagtgcgaa cttcaacgtt taagtcgatg tcgaccactt
     1321 gaactcctcg gtgttcaaaa aaatcgcgtg tcgtacggtg gcgatgccca tgtagatgga
     1381 ctccgtacca gccatttttg taagagtttt tacactcttt tcgaatgcgg cggtgatcct
     1441 gtactcggtg gcgatgaagg tctgcactaa agaagtactg atattcgata aacgtccagt
     1501 gcgaccagta ggtgtcgtga atcgcagctt ggtagccgag ttcaaatccg tcctgaaagc
     1561 cggctcggta cccctgttca aaacgcgaca gataacgatt ctcgaatgct gggttgtacg
     1621 cagaaattcc tatgaggccg ttggaataaa agaacgatcc gccgaagggg cgatagacgt
     1681 agtctggatc cggtacccag ccccacccga gattagcgaa gtgttcccag tgaccgtagt
     1741 ggtcggtgat ctcgccccag ggatcgagcg agtaccatac cgaaccatag gattcatggt
     1801 gataaaaata accgcgctga aatggagccc agcccatcgg agtgtcgtaa ggaaaccaaa
     1861 cccgaaggcc gccgacgacc gcccaagttc ccatgccgcg cagaaaatcg tcataagaaa
     1921 agtcactgcc gttgtagtcg tagtagtcgt catacgacgg ttcgacatat tccgtttcat
     1981 aaactacgtc gtgttccgca cgtgcgttcg aaatagtcat cgcgattccg acagaaaaca
     2041 tcgcgagcaa atagatacca aaaaaccgga cgcggtgggc gcccggcgtg caatgatggt
     2101 gtgggttcat tcgtttctcc ttcacaagaa cgggtaagaa cgggttttta aaatgcagtt
     2161 tttgcctatc gcacgaatcg ttccagatct ttatagtcag atgttctggt gtaacacgtt
     2221 gtttttaaac aagatgagat ttgtgtcaga ttgaatcact cagttacatt ttgctttttt
     2281 ggggtcgaag tgaccctgca tttgtgaaaa aataacggca tgccaaataa gacactctcg
     2341 agatttacga cttcgatttg caccgcgatc ggtgttggtt ttttgtttac ggcgtgtgcc
     2401 cacaaaccta atccttggcg cacggtgcgg gaaccactga aacaaaaagc ccaggtttat
     2461 ggaa
//
LOCUS       DAOMXO010000346         2460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_24620_length_2460_cov_5.570062, whole genome shotgun sequence.
ACCESSION   DAOMXO010000346 DAOMXO010000000
VERSION     DAOMXO010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24620_length_2460_cov_5.570062"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLH57_07620"
     CDS             <1..449
                     /locus_tag="PLH57_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRK02519.1"
                     /translation="AVVLMSGLFLGPWKGAVSQALYIGLGALGLPFFAAPAALAGPTG
                     GYILGFVLAAFLTGSLSQKIKDHSGVWNKIKTYGLVLIASLGIFVPGVLWLKLLTGQS
                     WSASLAMGFVPFIIGDLIKTAIAVSSYSVAAAAARAAGRIRKNTVF"
     gene            complement(386..1033)
                     /locus_tag="PLH57_07625"
     CDS             complement(386..1033)
                     /locus_tag="PLH57_07625"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HRK02520.1"
                     /translation="MSLYIAYLEWPPGEVVVTSPVRLPLFGALRIRRRRATLETMRIF
                     QKISRLRFMRSPRFAHKLLPKLLEYYPPFWIMGVRIKAVTPDFRKTHLSLPLKWYAKN
                     THGTMFGGYMCAVSDPVAAIMCGEIFRKRGVETWTKAHSVTFMKPGRTALEMKVEVTD
                     EDLAKINNDLDQHGRSTHVFEFFITDKAGVPVAKVQNTVFLRIRPAARAAAAATE"
     gene            complement(1239..1937)
                     /locus_tag="PLH57_07630"
     CDS             complement(1239..1937)
                     /locus_tag="PLH57_07630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRK02521.1"
                     /translation="MNSQKKIEQEALETVIVELERNLEVLELNYRKAVTNLKSLRERV
                     ETDDLTKLMRRGAFMRKLGDLLIDSKETGDEVQLMMIDIDHFKAVNDNYGHQTGDVVL
                     TRVAELVRQYLRTEDLAGRYGGEEIIVAMQVSSGEAQRIAENIRAAVQAQLQVSSARR
                     QDAERAEAKTFHVTLSMGIASTQDFTYEADVLIGEADAALYRAKRGGRNKVLRAKNIK
                     ALSIAELVLEKAAA"
     gene            2262..>2460
                     /locus_tag="PLH57_07635"
     CDS             2262..>2460
                     /locus_tag="PLH57_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02522.1"
                     /translation="MVYVSRSKLLRFFGILTVLAQSFSAVAEEDIKASSVDYENLSLT
                     FGKDNCIQATKDSPDANFYNYG"
BASE COUNT          518 a          604 c          637 g          701 t
ORIGIN      
        1 gcgctgtggt actcatgagc ggtttatttt tgggtccctg gaaaggggcc gtaagtcagg
       61 cgctctacat tgggctcgga gcgctgggac tccctttttt cgcggcacct gcggctctcg
      121 ccggtccgac gggcggttat atcttgggtt ttgttcttgc ggctttttta accggctcgc
      181 tttcacaaaa aataaaagat cattctggag tttggaacaa gatcaagacc tacggtcttg
      241 ttttgatcgc gagtctcgga atatttgttc ccggagtatt gtggcttaaa ttactcactg
      301 gccagtcatg gtctgcttcg ctggccatgg gtttcgttcc gtttataatt ggcgacttga
      361 ttaaaacagc tatcgcggtg agtagttatt cggttgcggc ggcggctgcc cgcgcggcag
      421 ggcgaattcg caaaaacacg gtgttctgaa ctttggccac cggaactcca gctttgtcgg
      481 ttataaaaaa ctcaaacaca tgagtggatc gaccatgctg gtcgagatcg ttgttgatct
      541 tggccaagtc ttcgtcggtc acttccactt tcatttctag cgccgtgcgt cccggtttca
      601 taaaggtaac ggaatgggct ttggtccaag tttcgactcc ccgcttacga aaaatttctc
      661 cgcacatgat cgctgccacc gggtctgaga cggcacacat ataaccaccg aacatagttc
      721 cgtgggtgtt cttggcgtac catttcaacg gcaagctcaa gtgggttttc cgaaagtctg
      781 gggtcacggc ctttatacgc acgcccatga tccaaaaggg tgggtagtat tctaaaagtt
      841 tgggtaaaag tttgtgggca aatcggggac tgcgcatgaa tcgtagtcgc gatatttttt
      901 gaaaaatgcg catggtttct agagtagctc gtcggcgccg aattctcaat gccccaaaaa
      961 gcgggagccg caccggggaa gtcactacga cttcaccggg cggccattct aggtaggcta
     1021 tgtataaaga cactcatact aactgacgtt tgagcattgg ggcgggcatc tccattgtgt
     1081 gaaacaactc gtgggtgggg acattcacca cgagccttgc aacagctagt tggttgcaat
     1141 tcttaaccaa gcgtttaagc tcggctcacc aagcgtttaa gctcagcaaa actttttctc
     1201 cgtcgccgac cgagtgttcc cctcggttgc ggccgtgatt aggcagctgc cttttccagc
     1261 accagctctg cgatcgacaa agctttgatg ttcttcgcac gaagcacttt gttgcgaccg
     1321 ccgcgtttgg cgcgataaag tgcggcgtcc gcttcaccga ttaaaacgtc ggcctcgtac
     1381 gtgaaatctt gggtagaggc gatacccatg cttagcgtga cgtgaaaggt tttagcctcg
     1441 gcacgttcgg cgtcttggcg gcgtgcgctt gaaacttgca gctgagcttg tacggcagca
     1501 cgaatgtttt cagcaattcg ctgagcttcg ccgctactaa cttgcatggc cacgatgatt
     1561 tcttcgccac cataacgacc ggccaagtct tcggttcgca ggtactggcg aaccaattct
     1621 gctactcgcg tgagcacgac gtctccggtt tggtgcccgt agttgtcatt caccgctttg
     1681 aagtggtcga tgtcgatcat catgagctgc acttcatcac cggtttcttt tgagtctata
     1741 agaaggtcac cgagttttcg catgaacgcg cctctgcgca tcaacttcgt gaggtcatcc
     1801 gtttccaccc gttcacgcaa actttttagg ttggttaccg ctttgcggta gttaagttcc
     1861 aacacctcaa ggttgcgctc taattccaca atcactgttt ctagagcttc ctgttctatt
     1921 ttcttctggc tgttcataac gactccctcg gtgacctttt tgtgagcagg gacgaagtgt
     1981 ttgtctttcg acttttttgg ggcaacccag agcaccgttt cagtgggttg ttacttcgtc
     2041 cctgttttgt ggctgctgtt tgcagccgcc tcacgactga tgggtattac atgccgcgta
     2101 ccaattctaa gtagttgatt ttgttggctg ccgtagtggc gagtgcgtcg tattcaccca
     2161 gtcgtgacgt ttaatcgaat agtgcctatc gtgggtccat ttgactcact tgtttgaggc
     2221 cacgctgttg atagactaga caaattgggg gactggcgaa aatggtgtat gtttctcgca
     2281 gtaaactttt acgttttttt gggatattaa cagtcttggc acagtctttc tcggcagtgg
     2341 ctgaagagga catcaaagct agcagtgtag actacgagaa cctcagtctc acgtttggca
     2401 aagacaattg catacaggct actaaagaca gtcccgatgc aaacttttat aattacggat
//
LOCUS       DAOMXO010000347         2459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_24636_length_2459_cov_5.072379, whole genome shotgun sequence.
ACCESSION   DAOMXO010000347 DAOMXO010000000
VERSION     DAOMXO010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24636_length_2459_cov_5.072379"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLH57_07640"
     CDS             <1..346
                     /locus_tag="PLH57_07640"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HRK02523.1"
                     /translation="IPRSGDFFRFFKTSQRKDLDISCVNAALFARKSNRGFSELRIAY
                     GGVAATPLRLKRVETLLRHQVIAADLIERAIAQLAKDITPRSDLRGSEAYRLRVSQNI
                     LRELLTEAAAHG"
     gene            339..>2459
                     /locus_tag="PLH57_07645"
     CDS             339..>2459
                     /locus_tag="PLH57_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRK02524.1"
                     /translation="MDNLSPSHDSARSHVTGTSEYVDDRPILPREVFVDVVHSTHAHA
                     RVLKIDTRAALALPGVVAAFTAKDFAELYWGSIFHDQPLLASDVVQFCGEPLVILAAE
                     SLDTLRAAKTLVEIRYEPLPAILTIHDAIRKKSFIAGERKIERGSSNDDTVPSALDSV
                     IADAPHQLEGTLTLQGADHFYLESQCAIAYPLENNTIEVHSSSQHPTEVQHVIAHALG
                     LADHRVTCVVKRMGGAFGGKESQAAPFAAYAALVAQKLQRPARIAISKDDDMKITGKR
                     NPFFISYRVGFKNDGTLLALDARLFSDGGAYADLSTAIMERALLHSDNAYFVPHLRVR
                     GQVCKTNTHPSTAFRGFGGPKGVTMIEKIIEEIAERLNMDPLDVRQKNVYRPGYDVTH
                     YGQKIENNVLPALFEQLEVACDYRRRRAEISDWNATKAIAQKELRGLSLTAVKFGISF
                     TTRHLNQANALVVVHRDGTVQVSTGATEMGQGVNTRIAQLVAEVFGISHNDVRVLATT
                     TDKNGNTSPTAASSGTDLNGSAAVLAARRIRARLNQVAAHMRRTERDRWATFTAGLGT
                     EKEFELSEDSSDSFMDMFPFREIVTEAYYNRVSLSEYAHYKIPNLGFNKVTGQGRAFL
                     YYTQGAAATEISINTVTGEVKILRTDILMDLGRPINRALDLGQIMGGYIQGLGWMTTE
                     NLFYDDHGALVSHSPSTYKIPNIQD"
BASE COUNT          607 a          706 c          594 g          552 t
ORIGIN      
        1 gattccccgt agcggcgact tctttcgttt ttttaaaacc tctcaacgca aagacctcga
       61 tatatcgtgt gtgaacgcgg cccttttcgc acgcaaatct aaccgggggt ttagtgagtt
      121 gcgcattgct tacggcggcg tcgcggccac gcccttgcga ctcaagcgcg ttgaaacatt
      181 acttcgtcat caagtgattg cggccgattt gattgaacgc gccattgccc agctggcaaa
      241 agacatcaca cctcgaagtg acctacgcgg aagcgaagcc taccgtttgc gcgtgagtca
      301 aaatatctta cgcgaacttc tgacagaggc ggccgcccat ggataatctt tcgccaagcc
      361 atgactccgc gcgaagtcat gtaacaggaa ctagtgagta cgtggacgat cgtcccattc
      421 tacctcgcga agtgttcgtc gacgtcgtac acagtaccca cgcccatgcc cgcgttctca
      481 aaatcgatac tcgggccgcc ttggctctgc ccggagtggt ggcggccttt accgcaaaag
      541 attttgccga attatactgg ggcagtattt ttcacgacca acccttactc gcctccgatg
      601 ttgtgcagtt ctgcggcgaa ccactggtaa ttctcgcagc cgaatctctc gatacgcttc
      661 gagctgcgaa aaccctggtc gaaataagat acgaacctct acccgcgatt ctcacaattc
      721 acgacgcaat ccgaaagaaa agctttatcg ccggcgaacg caagatcgaa cgcggctcga
      781 gcaacgacga cacagtgcca agcgcactcg actcagtaat tgccgacgct ccccatcagc
      841 tcgaaggcac gctaacactt cagggagctg atcatttcta cctcgaaagt cagtgtgcca
      901 tcgcctaccc attagaaaac aacacaatag aagttcattc gtcgtcgcag catcccaccg
      961 aagttcagca cgtcatcgca cacgcgctgg gcctcgccga tcatcgggta acttgtgttg
     1021 taaaacgcat gggcggcgct ttcggtggaa aagaatctca agctgctccg tttgcggcct
     1081 acgccgcact cgttgctcaa aaactgcaac gacctgcgcg tatcgcgatt tctaaagacg
     1141 acgatatgaa gatcaccggc aagcgaaacc ctttttttat ttcttaccgc gtaggtttca
     1201 agaacgacgg caccctgctc gcacttgacg ctcgactttt ttccgacggt ggcgcctacg
     1261 ccgatctctc cacggccatc atggagcgag cacttctaca cagcgacaac gcatattttg
     1321 tgcctcattt gcgggttcgc ggccaggttt gcaagaccaa cacccaccct tccaccgctt
     1381 ttcggggctt tgggggccct aaaggcgtga ccatgatcga aaaaattatc gaagaaatcg
     1441 ccgaacgcct gaacatggac ccgctcgatg tacggcaaaa aaatgtatac cgcccgggat
     1501 acgacgtcac ccattacggt cagaaaattg agaacaatgt tttacctgct ttatttgaac
     1561 aactcgaggt cgcgtgtgat tatcgtcgtc ggcgcgccga gatttctgac tggaacgcca
     1621 cgaaagctat cgcacaaaaa gaattgcggg ggctttcgct tacggccgtg aagtttggaa
     1681 tatcgttcac cacgcgccat ttgaaccagg ccaacgctct tgttgtcgta caccgcgacg
     1741 gcacggttca ggtgtccaca ggcgccactg aaatgggtca aggcgtgaac acccgcatcg
     1801 cgcaactggt ggccgaggtt tttggaattt cacacaatga cgtgcgggta ctcgcaacta
     1861 ccaccgataa aaacggtaac acctcaccaa ccgctgcctc cagcggcacc gatttaaatg
     1921 gctctgccgc cgtactcgcc gcacgccgca ttcgggcgcg cctcaatcaa gtagcagctc
     1981 acatgcgccg cactgagcga gaccgttggg ctacattcac ggcgggactc ggaacagaaa
     2041 aagaatttga actaagcgaa gacagcagtg attcgtttat ggatatgttt ccgtttcgcg
     2101 aaatcgtcac cgaggcctat tacaatcgtg tttccctttc agaatacgca cactataaaa
     2161 tccccaatct aggatttaat aaggtcaccg gccagggccg cgcatttttg tattataccc
     2221 aaggtgccgc ggctaccgag ataagtatta ataccgtgac tggcgaagtg aaaattttgc
     2281 gtacggacat tctcatggat cttggccgcc caatcaaccg cgcactcgat ctagggcaga
     2341 ttatgggtgg ctatattcaa ggtctcggat ggatgacgac cgaaaatctt ttttacgacg
     2401 accacggggc gctggtttcc cacagtccga gcacctacaa aattccgaac attcaagat
//
LOCUS       DAOMXO010000348         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_24788_length_2449_cov_5.060568, whole genome shotgun sequence.
ACCESSION   DAOMXO010000348 DAOMXO010000000
VERSION     DAOMXO010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24788_length_2449_cov_5.060568"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PLH57_07650"
     CDS             complement(<1..133)
                     /locus_tag="PLH57_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRK02525.1"
                     /translation="MARYCESVCRICRRENQKLFLKGDRCYTEKCSFERRGYPPGQHG
                     "
     gene            complement(137..595)
                     /gene="rpsK"
                     /locus_tag="PLH57_07655"
     CDS             complement(137..595)
                     /gene="rpsK"
                     /locus_tag="PLH57_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRK02526.1"
                     /translation="MSEVKQEKEEKVAAPAPVEGTAAAAGETTTRKKKTKKNVSTGNV
                     YIQASFNNTIVTITDSMGNVVCWSTAGAKGFRGSRKNTPFAAQVAAEDAARKAADNGM
                     RTVQVFVNGPGAGRESAVRAMQAAGLRVTFLKDITPIPHNGCRPPKRRRV"
     gene            complement(630..1013)
                     /gene="rpsM"
                     /locus_tag="PLH57_07660"
     CDS             complement(630..1013)
                     /gene="rpsM"
                     /locus_tag="PLH57_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRK02527.1"
                     /translation="MARISGIDLPRNKRMEIALTYIYGIGRSSAKRILAAANVDGNKK
                     SDTLTEVEVSRIRDVLDKSERVEGDLRRETSLNIKRLVDLGCYRGTRHRKGLPVHGQR
                     THSNARTRKGPAVAIAGKKSVKAMK"
     gene            complement(1034..1147)
                     /gene="rpmJ"
                     /locus_tag="PLH57_07665"
     CDS             complement(1034..1147)
                     /gene="rpmJ"
                     /locus_tag="PLH57_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRK02528.1"
                     /translation="MKVRASVKKICDKCKVIRRVGVLRVICSNPRHKQRQG"
     gene            complement(1163..1381)
                     /gene="infA"
                     /locus_tag="PLH57_07670"
     CDS             complement(1163..1381)
                     /gene="infA"
                     /locus_tag="PLH57_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRK02529.1"
                     /translation="MAKEDAIEVEGTVLEPLPNAMFRVELANGHKILAHISGKMRMHY
                     IKILAGDKVTVELSPYDLTRGRITYRVK"
     gene            complement(1416..2165)
                     /gene="map"
                     /locus_tag="PLH57_07675"
     CDS             complement(1416..2165)
                     /gene="map"
                     /locus_tag="PLH57_07675"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRK02530.1"
                     /translation="MIHLKSPQEIEIMRRAGRATANILREMAKNVRPGITTGELDQIA
                     EKMCKELGVKPAFKGYKGFPACVCISVNDQVVHGIPGDRVLENGDIVGLDFGVIHEGY
                     HGDSAITVPVGTITPQAQALVDATRECLMKGIEQAVVGNKLFDISHAVQNYVEGLGFS
                     VVREFVGHGIGKSLHEDPQVPNYGPKGRGVPLKEGLVLAIEPMINAGTHAIKVESDGW
                     TAVTIDGSLSAHFEHTIAITKNGPEILTLPN"
     gene            complement(2177..>2449)
                     /locus_tag="PLH57_07680"
     CDS             complement(2177..>2449)
                     /locus_tag="PLH57_07680"
                     /inference="COORDINATES: protein motif:HMM:NF017042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRK02531.1"
                     /translation="IANTFCRQCGTGYHVDFQPPRAEGVCDRCNGELFQRNDDVAEVI
                     VDRLRVYNDSTAPLLKYYQDKGALRAIDGTGSMTQITATIIGYLEK"
BASE COUNT          586 a          669 c          495 g          699 t
ORIGIN      
        1 ggccgtgttg acctggggga tatccacgtc tttcgaatga acacttctca gtgtaacaac
       61 gatcgccttt caaaaagagt ttttggtttt ctcttcggca aatacgacaa actgattcac
      121 aataacgagc catgaattaa actctcctac gcttcggtgg tctgcaccca ttgtgtggaa
      181 taggtgtaat atcttttaaa aatgtaacgc gaagaccagc tgcttgcatt gcccgcacag
      241 cagattcacg acctgcaccg gggccattca cgaaaacttg tactgttcgc attccgttgt
      301 cggcggcctt tcgggcagcg tcttcagctg cgacttgcgc cgcaaaggga gtgttcttgc
      361 gagatcctcg aaaacccttt gctccagcag tagaccaaca aaccacgtta cccatcgagt
      421 cggtaatggt tacaatcgtg ttattgaaag acgcctgaat ataaacattc ccagtagaaa
      481 cattcttttt cgttttcttc ttacgagttg ttgtttcacc ggctgcagcg gcagttccct
      541 cgacgggagc tggtgcggcg accttctctt ctttttcttg tttcacttcg ctcattcggt
      601 ctccttaaaa aatcttctct actcgaactt tacttcatcg ccttaacaga ctttttgcct
      661 gcgattgcga cagctgggcc ctttcgtgtg cgggcgttcg aatgggttct ctggccatga
      721 actggcaagc ccttacgatg acgagttccg cgataacagc ctaagtcaac gagtcgcttg
      781 atattcaggc tcgtttcacg acgcaaatcg ccctcaacgc gctcactttt atccaaaacg
      841 tctcggatgc gagacacttc gacttcagtc aaagtgtcac tctttttatt tccatcaacg
      901 tttgctgctg cgagaattct cttcgcggaa gatcgcccga ttccgtaaat atacgttaac
      961 gcgatttcca ttcttttgtt tctgggaaga tcgataccag atatgcgtgc cactgttcac
     1021 tccttattaa tgactaacct tgacgctgct tgtgccgcgg attcgaacaa atcactcgta
     1081 gaaccccaac tcggcgaata actttgcact tgtcgcaaat ttttttaaca gacgctcgta
     1141 ctttcattcc acacctacca atctatttta cgcgatatgt aattctaccg cgggtcaaat
     1201 catatggaga caattcaacc gtaactttgt ctccggccaa aatcttgatg taatgcatcc
     1261 gcatttttcc ggaaatatgg gcaagaatct tatgtccatt tgctagttcc actcgaaaca
     1321 tcgcattagg tagaggttca agcactgttc cctctacttc aatggcatcc tcttttgcca
     1381 tgccctctct tctcccacaa aaacagctcc cgccatcaat tcggaagcgt taatatctcc
     1441 ggaccgttct tcgttatcgc tatcgtatgt tcaaagtgcg ccgacaacga accatcgatc
     1501 gtaaccgccg tccaaccatc cgattctact ttaatcgcat gtgttccagc gttgatcata
     1561 ggctcgatag ccagtaccag accctctttt aaagggacac ctctcccctt aggcccataa
     1621 tttggaacct gggggtcctc gtgcagactc ttacctatgc catgaccgac gaactccctt
     1681 accacggaaa atccaagccc ttcaacataa ttttggactg catgagagat atcgaacagc
     1741 ttatttccga ccaccgcttg ctcgattcct ttcattaaac actctcgggt agcatccact
     1801 aaagcttggg cctgcggggt gattgttcct acggggacag ttatagccga gtctccgtgg
     1861 tacccttcat ggatcacccc aaaatcaagc cctacgatgt caccgttctc aagaacccgg
     1921 tcccctggta ttccatgaac gacctggtca ttgacactta tacagacaca agctgggaaa
     1981 cctttgtaac ctttaaaagc aggttttacg cctaattcct tgcacatttt ttcggcaatt
     2041 tgatcaagtt caccggttgt aatccccggc cgaacatttt tcgccatttc cctcaagatg
     2101 ttcgcagttg cacgacctgc tcggcgcata atttcgattt cttgggggga ttttaggtgt
     2161 atcacgccat cacttcctat ttttccaaat aaccaataat agtggcggtg atttgggtca
     2221 tagaacccgt tccatctatc gctcgaagag cacccttatc ctggtagtac ttcaacaacg
     2281 gcgctgttga atcattatag actcgcaaac ggtctacgat tacttcggca acatcgtcgt
     2341 tacgttgaaa aagctctcca ttgcaccgat cgcaaacacc ttctgctcgc ggaggttgaa
     2401 aatcgacgtg atacccagtg ccacactggc ggcagaaggt gtttgcgat
//
LOCUS       DAOMXO010000349         2447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_24815_length_2447_cov_4.192726, whole genome shotgun sequence.
ACCESSION   DAOMXO010000349 DAOMXO010000000
VERSION     DAOMXO010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24815_length_2447_cov_4.192726"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLH57_07685"
     CDS             complement(<1..278)
                     /locus_tag="PLH57_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02532.1"
                     /translation="MGTRLTKTFKNRMTPKTFRKHLLGLSAALVFFIFAGVTKKLWVS
                     GEGMLSSSLNRDVLRSVLGKKIISARSLDSAAGLSIDQEDFQVEYSID"
     gene            complement(332..1135)
                     /locus_tag="PLH57_07690"
     CDS             complement(332..1135)
                     /locus_tag="PLH57_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRK02533.1"
                     /translation="MKRFAKIHANRIYAVFVLVAVLTGAFLSYSGARIGYSTYRRVFP
                     DISALKHSYPVAVEKKDGKITYKLVYERPERWRSVGQISKKAVSAILAAEDATFWKHG
                     GYSAEAIRAAIEHNAKPNAKIKRGGSTITQQVVKNVFLSPEKTISRKLRELLLSVELE
                     RKLPKAKILEIYLNIAEWGPGVYGIEAASWRYFDKPSSLLTAREGAILAFMLPNPIRY
                     KNSYRDGELTKFGSNRVAKILNRLWRSGKISEDEYSASGEPEDQVSSEL"
     gene            complement(1107..1595)
                     /locus_tag="PLH57_07695"
     CDS             complement(1107..1595)
                     /locus_tag="PLH57_07695"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRK02534.1"
                     /translation="MENQKPPFHYHLVLVEPEIPPNTGNAGRLCVATQSTLHIAGTPG
                     FSLDDKDVRRAGLDYWKHVDLKRHESFEKWLEWWGAGHGSSPFFLLSKKAKKSLFDVK
                     FPKEAAFVFGKETLGLPESLLDRYAEQSYNIPMFSPLIRSLNLSNAVSIVLYEAIRQN
                     TR"
     gene            complement(1608..>2447)
                     /locus_tag="PLH57_07700"
     CDS             complement(1608..>2447)
                     /locus_tag="PLH57_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRK02535.1"
                     /translation="FDAPILTPSACEGTSTLFSTPYFDEKAYLTQSGQLYGEVGAMAF
                     GKIYVFGPTFRAEKSKTRKHLTEFWMVEPEVAWNDLNDNMQLAEAFIQHIVQRVLEKR
                     GAELKVLERDLSKLEKIRAPFPRIRYDEALKVLEKAGVGVPFGEDFGAPQESALGEAF
                     DGMPVFIHRFPHEIKAFYFKRDPEDARFALGCDLIAPDGYGEIIGGGQREESAAVLKE
                     RIAEHKLPEKDYQWYLDLRRFGSVPHSGFGLGVERTVAWLCGLDHVRETIPFPRMINT
                     IKP"
BASE COUNT          625 a          581 c          595 g          646 t
ORIGIN      
        1 tggtcaatgg aatactcaac ctgaaaatct tcttgatcga tcgaaagacc ggctgccgaa
       61 tcaagtgacc gggcggaaat aatttttttt ccgagaaccg agcgaagtac gtcgcgatta
      121 agcgacgaac tgagcatgcc ttcgccagat acccaaagct ttttggttac acctgcgaaa
      181 ataaaaaaca ccaatgctgc actgagtcca agtagatgct ttcgaaaggt tttaggtgtc
      241 attcggttct taaaagtttt agtaagtcgg gtacccattt agtaagcttt tcggaccctt
      301 acccccaaac ttgagcacga aactcaagtt gttacaactc tgaagatacc tgatcttcgg
      361 gttcaccgga agcagagtat tcatcttcgc tgatttttcc gcttcgccaa aggcgattta
      421 gaatcttggc aactcgattt gaaccaaatt tggttaattc gccgtcacgg taggaatttt
      481 tgtaccgaat gggattgggt aacatgaagg caagaattgc cccctcgcgg gccgttaaaa
      541 gggatgatgg tttgtcaaaa taacgccaac tggcggcttc gattccgtaa acgccggggc
      601 cccactcagc aatattgagg tagatttcaa gtatcttagc cttcggcagt tttcgttcga
      661 gttcgacgga aagtagaagc tccctcagct ttcgtgaaat ggtcttctca ggactaagaa
      721 aaacgttttt tacgacctgc tgagtaattg tactgccgcc acgttttatt ttagcgttcg
      781 gcttcgcatt gtgttcaatg gccgcacgaa ttgcttcagc cgaatagcct ccatgtttcc
      841 aaaaggtggc gtcttcggcc gcgagaatcg ccgaaactgc ttttttagaa atttgcccaa
      901 cggagcgcca acgctccgga cgttcgtaaa cgagtttgta ggtgatttta ccgtcttttt
      961 tctcaaccgc gaccggatag ctatgtttaa gcgcactaat gtcgggaaac actcggcgat
     1021 aggtggaata accgattcgc gcgccactat aggacaaaaa ggctccagtc aaaactgcaa
     1081 ccaatacgaa gaccgcatat attcggttag cgtgtatttt ggcgaatcgc ttcataaagc
     1141 acaatcgaaa cggcgttcga taaatttaaa ctacggatga gcggcgagaa cattggaata
     1201 ttgtagctct gttccgcgta tcgatcgaga agtgattcgg gcaggccgag cgtttccttt
     1261 ccaaaaacaa aggcggcttc cttgggaaac ttcacgtcga ataggctttt cttagccttt
     1321 ttcgagagta aaaaaaaggg cgatgatccg tgtccagccc cccaccattc tagccatttt
     1381 tcgaaagatt cgtggcgttt taggtcgacg tgcttccagt agtcgagccc cgcgcgtcga
     1441 acgtccttat cgtcgagact aaacccagga gtgcctgcga tgtgtagcgt actttgagtg
     1501 gctacacaca gacgcccggc attgccggtg ttaggcggga tctcgggttc aactagaacg
     1561 aggtggtaat gaaatggcgg cttttgattt tccacgtcta aatacttcta cggctttatt
     1621 gtattgatca ttcgcggaaa cggaatagtt tctcgcacgt gatcaaggcc gcaaagccat
     1681 gcgaccgtac gttcaactcc gagtccgaaa cccgaatgag gtacggagcc gaagcggcgt
     1741 agatccaggt accactgata gtctttttca ggcagtttgt gttcggcgat gcgttctttt
     1801 agtacggcag cgctttcttc gcgttggccg ccgccgataa tttcgccgta gccatctggt
     1861 gcaatcaagt cacatcccag agcaaatcgc gcgtcttcgg ggtcacgctt gaaataaaac
     1921 gccttaattt cgtgagggaa acgatgaata aataccggca tgccatcaaa cgcttcaccc
     1981 aaagcagatt cttggggtgc cccaaagtct tcaccaaatg gaacgcctac gcccgctttc
     2041 tcgaggacct tcaatgcttc gtcatagcga atgcgcggaa atggggcgcg aattttttca
     2101 agtttagaga ggtctctttc gagtaccttt agttccgcac cgcgtttttc gagcacacgt
     2161 tgaacaatgt gctgaataaa ggcctcagcg agttgcatat tgtcattcaa atcgttccaa
     2221 gccacttcgg gttcgaccat ccaaaattcg gtgaggtgct ttcttgtttt tgatttttca
     2281 gcacgaaacg taggaccaaa aacataaatt ttcccaaagg ccatggcgcc cacttcgccg
     2341 tacaattgac cggactgggt gagataggct ttttcatcga agtaaggagt tgaaaaaagc
     2401 gttgaagttc cttcacaggc cgatggggta agtattggag cgtcgaa
//
LOCUS       DAOMXO010000350         2435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_24992_length_2435_cov_4.697059, whole genome shotgun sequence.
ACCESSION   DAOMXO010000350 DAOMXO010000000
VERSION     DAOMXO010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24992_length_2435_cov_4.697059"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLH57_07705"
     CDS             complement(<1..835)
                     /locus_tag="PLH57_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HRK02536.1"
                     /translation="MESSNQAEVQNSLEAGLPTWESLVNNYLLVSEARAHIEALQRLK
                     SPKIVKQLRPLMTGLFTLFSGAIRRPNLPDLVNTHWNELVDLASEQVVRNIHHANSNS
                     LTTGMNSSSILNHKFNQLPCSQKNLLERADLIAEYIGCDAPVLLLGDDDLLSLELAKR
                     GFKNVTVVDIDPTIIQRIQHIAAQHDFPIRAKVHDLQMPPSADLIQNYGLIFMDPPCT
                     VAGLKLFVGSALTFCSALRTTKFIACTHSMSLLQPGIEEFRNYLSSVGLRVTAYKPEF
                     AV"
     gene            complement(813..1451)
                     /locus_tag="PLH57_07710"
     CDS             complement(813..1451)
                     /locus_tag="PLH57_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRK02537.1"
                     /translation="MEVIDVLAFALCGFTGGIVSSAPIGPINLQVLQMHRSGRSTFAF
                     VLGVVLADVALAGLAYFGYAKFISKQQTEYLFLIAGFLLICLGLKEHLQRKQPPRTDV
                     RMREARFRHFDFWFGASICLSNPTFLAFWVLWTTSTLPVFSPSATALTPVVFLSGIFM
                     GDFLWFSFLGRFARVTKSSRTLALIQRFTAGLTILFGGFLLAKGFYGIFKSS"
     gene            complement(1442..1825)
                     /locus_tag="PLH57_07715"
     CDS             complement(1442..1825)
                     /locus_tag="PLH57_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HRK02538.1"
                     /translation="MNTFGKHLILDIWLQDKITAFTVDSILDLVRSQFTVVAETNHPF
                     EPQGLTHVCILAESHFTIHTYPEHQYLSMDLYICNDGFDLGVFEKTILALFKINRYRS
                     ETILRGRGAAQRLPDFSEQRVEEWK"
     gene            complement(1883..2218)
                     /locus_tag="PLH57_07720"
     CDS             complement(1883..2218)
                     /locus_tag="PLH57_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02539.1"
                     /translation="MGFYRAAQWMVLLVFVVEVLNSTTVFVRKSSVATTHSQNTPQFY
                     FQDNTDAPHTLDQSSTPEEVDYLSAEELATDSETSNKTLLTKRHRLPANLAPPQEFYQ
                     SIHRPPLRS"
BASE COUNT          656 a          509 c          648 g          622 t
ORIGIN      
        1 acaccgcgaa ctctggtttg taggcggtca cgcgcagccc gacacttgag agatagtttc
       61 gaaattcttc aatacccggc tgcagcagtg acatgctgtg ggtgcaggcg atgaatttgg
      121 tagttctaag tgctgagcag aaagtcagcg cagatcccac aaacaatttt aatcctgcta
      181 cagtgcacgg cgggtccatg aaaatgagac catagttctg aattagatct gcgctgggag
      241 gcatttgaag atcgtggact ttcgctcgaa taggaaagtc gtgttgagca gcgatatgct
      301 gaatgcgttg tatgattgtg ggatcgatgt cgactacggt tacattttta aatcctcgtt
      361 ttgcgagttc cagacttaat aggtcgtcgt cgcctaaaag caaaactggt gcgtcgcagc
      421 caatgtattc cgcaatcaga tcggcgcgtt cgagtaagtt tttttgactg caggggagtt
      481 ggttgaattt gtggttcaga atggaactcg aattcatacc cgtggtcaaa gagttgctgt
      541 ttgcatggtg aatgtttcgc accacctgtt ccgaagccag atccacgagt tcgttccagt
      601 gagtgttcac caaatctggc agattggggc ggcgaatggc cccgctaaaa agagtaaaca
      661 agcccgtcat gagggggcga agttgtttta cgatcttcgg tgatttgagt cgctgaagag
      721 cttcaatatg ggcgcgagct tctgagacga gcaggtagtt gtttacgagc gactcccagg
      781 tgggaagccc cgcttcaagt gagttttgga cttcagcttg atttgaagat tccataaaac
      841 cctttcgcca gcagaaaacc gccgaataag atagttaggc ccgctgtaaa tctttgtatc
      901 aatgcgagcg tacggcttga tttagtaact ctggcaaacc gcccgagaaa tgaaaaccat
      961 aggaagtctc ccataaatat tccgctaaga aaaactaccg gagtaagtgc cgtggcagaa
     1021 ggggaaaaaa caggcaaagt ggaggtcgtc cacaagaccc agaatgccaa gaatgtcgga
     1081 ttcgataggc aaatgctggc accaaaccag aagtcgaagt ggcggaatcg cgcttctctc
     1141 atgcggacat cggttcgggg aggttgtttt ctttgaaggt gttcctttaa gccaaggcaa
     1201 atgagcaaaa aaccagcaat caaaaataga tattcggttt gctgcttaga aatgaactta
     1261 gcgtaaccga aataagcgag accggcgagt gcgacgtcgg caagtaccac gcccaaaacg
     1321 aacgcaaagg tactgcggcc ggaacggtgc atttgcagaa cttgtaaatt tattgggccg
     1381 attggtgcgg aggaaacaat accgcctgtg aaaccacata aagcgaatgc gagtacgtct
     1441 attacttcca ctcttcgact cgctgttcgg aaaaatctgg cagacgttgc gcagcaccgc
     1501 gacctcttag aattgtttcg ctgcgatatc gattgatttt gaacaatgcc aaaatagttt
     1561 tttcaaacac cccaagatca aaaccatcgt tgcagatata gaggtccatg cttaagtatt
     1621 ggtgctcggg gtaagtatga attgtgaaat gagactccgc cagtatgcaa acgtgagtga
     1681 gcccctgggg ctcgaaaggg tggttagttt ccgccaccac agtaaactgc gaacgaacca
     1741 aatctagtat ggaatcaacg gtaaaggcag ttattttgtc ctggagccaa atgtcgagta
     1801 tgaggtgttt gccgaaggta ttcatgttgt attccgccct ttttgcctgc gctttattca
     1861 gtagagaaat atagaagatg ttttaactgc ggagcggtgg acggtggatt gactggtaaa
     1921 attcctgcgg cggtgcaaga ttggcgggta atcggtgacg ctttgtaaga agtgttttgt
     1981 tggacgtttc agaatcggtg gctaattctt cggcgctcag atagtcgacc tcttcgggag
     2041 tgctactttg gtcgagggtg tggggggcgt cggtgttgtc ctgaaaatag aactggggag
     2101 tgttttgaga gtgagtcgtg gctacagagg atttacgcac aaacactgtt gtcgagttta
     2161 agacctcgac gacaaaaacc aacaacacca tccattgcgc tgcacggtaa aagcccatct
     2221 tcctataagc ttaagttcat ttttaaaaac tgacaaggtt atcgccgcat ggcgggatgt
     2281 tgtttgcctt tcttatcctt ttttcggcaa taaccttcaa gaattcattg tgaaaggcct
     2341 tgcaagcctt tcggtgtgaa agttcggtct aatatttcaa aaaaaatgtg aacgcccaat
     2401 gccataaggg cggcgggaat agcaccacgt aagat
//
LOCUS       DAOMXO010000351         2435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_24994_length_2435_cov_4.368487, whole genome shotgun sequence.
ACCESSION   DAOMXO010000351 DAOMXO010000000
VERSION     DAOMXO010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24994_length_2435_cov_4.368487"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLH57_07725"
     CDS             <1..274
                     /locus_tag="PLH57_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HRK02540.1"
                     /translation="NTPFLTITKVEVMPDLRNASVWISSMELDPEKEEDEKRIRKLED
                     ALNRAAGYLRHQLVQSLNTKVTPALHFRFDRGFCNTMRVNTLLAKI"
     gene            295..1227
                     /gene="truB"
                     /locus_tag="PLH57_07730"
     CDS             295..1227
                     /gene="truB"
                     /locus_tag="PLH57_07730"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRK02541.1"
                     /translation="MKSGIILVDKPAGSTSFDCIRDLKRAWKRSDLGHCGTLDRFATG
                     LLPILVGDGLKLVRFFLESYPNLPTYWKTYQGVLLLGRQTATGDPEGEVLHDLPVPES
                     IDRSKIEEAMKTFENAPYAQTPPAYSAKKIEGSRASDLAREGKLNPESLKAVDVTIEL
                     FRLIDLTREGDKVRVRFEARCSKGTYVRRLAEDLGQKLGTHGTLLELCRTHVGSFKLS
                     DAKPLSELCTTAADDAILDMGVATSFLPRMIALDADVEAMKKGQFAGLHARLANADFP
                     PNVYCAATVAGDRPVALLELRSDQRIDFLRAFQI"
     gene            complement(1234..>2435)
                     /gene="hflX"
                     /locus_tag="PLH57_07735"
     CDS             complement(1234..>2435)
                     /gene="hflX"
                     /locus_tag="PLH57_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRK02542.1"
                     /translation="KTYLGSGKLEELRKQMDELELDLLLVDFDLSPSQLKNVEKITQK
                     LVLDRSGIILEIFNRHARSKEAKLQVETARLQYLMPRLTNLWSHFERQRGSGGGALKG
                     KGMGEKQIEVDRRLVKDRLTHLRRKLVEVEASRDLHRRQRESILKVALVGYTNAGKST
                     LLNALTKSEVLVEDALFATLDASVRLLTPKARPAVLAIDTVGFIDRLPHGLVASFRST
                     LGEVLEADLLVHVLDGSSEEVQRHCEVTMKVLAELGAHQIPMLLVFNKMDQCKKTQML
                     KIWANGLYRETLDANEIAMQNLNGAEPPRFHIPKEGAALFKPVFLSALDLSQVSQLRE
                     RILSIFDSQLEIYELVVPFEDGKTVSQIFDLSRVEVRRPLETGTFFRIRTLPEFAARI
                     NVKRYQA"
BASE COUNT          630 a          648 c          561 g          596 t
ORIGIN      
        1 gaacactcct tttctaacga tcactaaggt cgaagtaatg ccagatcttc gcaatgcgag
       61 cgtttggatt tcttcaatgg aattggatcc tgaaaaagaa gaagacgaaa aacggattcg
      121 caaattggag gacgcgttga atcgagcggc cggctatttg cgccatcaac tcgttcaatc
      181 gctcaacacc aaagttacgc cggcgttgca cttccgattc gatcgcggat tctgcaacac
      241 tatgcgcgta aacacacttc tcgcaaaaat ttgacccatc gggagcatct tcatatgaag
      301 tctggaatta ttctggtcga caagcccgct ggctctacat ctttcgattg tattcgcgac
      361 ctcaagcgcg cgtggaaacg ttccgatttg ggccactgcg gaacactcga tcgctttgct
      421 acgggcctac tcccgatatt agttggcgac ggattaaaac tcgtacgatt tttcttagag
      481 tcctacccaa atctcccgac ttattggaaa acctaccaag gagtcctttt acttggtcgg
      541 caaaccgcga ccggagatcc ggaaggcgag gtactccacg atcttcccgt tcccgagtct
      601 atcgatcgct ctaagattga agaggccatg aagacgtttg aaaacgcgcc ctacgcgcaa
      661 acgcctccgg cctacagcgc aaaaaaaata gaaggatccc gagcaagcga tttagcccgt
      721 gaaggtaaac tcaatccaga atcacttaaa gcggtcgacg tcaccattga actatttcga
      781 ctcatcgatt taaccagaga aggcgacaaa gtgcgggtcc ggtttgaagc tcgttgttca
      841 aagggcacat atgttcgtcg cctggccgaa gacctcgggc aaaaactcgg cactcatgga
      901 acgcttctcg aactttgccg cactcacgta ggctccttca aactctcaga tgcaaaacct
      961 ctttcggagc tttgcaccac ggccgccgac gacgcgattc tcgatatggg cgtggcaacg
     1021 agctttctac ctcgaatgat agccctcgac gccgatgtgg aagccatgaa aaaagggcag
     1081 tttgccggac ttcatgcaag gctcgcgaac gcggatttcc caccaaacgt ttattgcgcc
     1141 gctacagtcg cgggtgaccg ccccgttgca ctcctcgaat tgcgttcaga ccaacgaatc
     1201 gattttctgc gcgcgtttca aatctaagtt ctgttaggct tgataccgtt tgacgttaat
     1261 tctcgccgcg aactccggta gagtgcgtat tctaaaaaaa gttccggtct ccagcggtct
     1321 ccgcacctct acgcgcgaaa ggtcaaaaat ttgagaaacg gtttttccat cttcgaacgg
     1381 aacaaccaac tcataaattt ccagttgaga atcgaaaata ctgagtattc gttcgcgaag
     1441 ctgggaaacc tgagaaagat ctagggccga taaaaataca ggtttaaaaa gggcagcccc
     1501 ttctttcgga atgtgaaatc tgggcggttc cgccccattg agattttgca tagcgatttc
     1561 gttggcatcc aaggtctcac gatacaagcc gttggcccag atcttgagca tctgggtttt
     1621 cttgcattga tccattttat tgaacacaag caacattgga atctgatgag ctccgagttc
     1681 ggcgagaacc ttcatggtca cctcacagtg gcgctgcacc tcttcactag aaccatcgag
     1741 aacgtgaacc aacaggtcgg cttcaagcac ctcccctaag gtcgagcgaa aactcgcaac
     1801 taatccgtgc ggaaggcgat caataaaacc aacggtatca atggccaaaa cggcgggtcg
     1861 agccttcgga gtgagcaagc gcaccgaagc gtcgagcgtg gcaaaaagag catcttccac
     1921 taaaacctca gactttgtga gcgcatttaa cagcgtcgac ttgccagcgt tagtgtaccc
     1981 caccaatgcc accttcaaaa tgctctcgcg ctgacgacgg tgcaaatctc gagaagcttc
     2041 tacttctact aactttcgac gcaagtgggt aagacggtct ttcaccaaac gtctatcgac
     2101 ctcaatttgt ttttcaccca ttccctttcc tttgagtgcg ccgccaccgc taccgcgctg
     2161 tcgctcaaag tgggaccata agtttgtgag tctcggcatc aaatactgca acctggccgt
     2221 ctctacttga agtttggcct ctttgcttcg ggcatgccga ttgaaaattt cgagaataat
     2281 accgcttcga tctaatacga gtttttgagt aattttttcg acgtttttca gttgagaggg
     2341 cgaaagatca aagtctacca acaaaagatc gagttcgagt tcgtccatct gtttacgcaa
     2401 ttcctcgagc ttccctgagc ccaagtatgt ctttg
//
LOCUS       DAOMXO010000352         2433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25025_length_2433_cov_4.821699, whole genome shotgun sequence.
ACCESSION   DAOMXO010000352 DAOMXO010000000
VERSION     DAOMXO010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25025_length_2433_cov_4.821699"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLH57_07740"
     CDS             <1..342
                     /locus_tag="PLH57_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02543.1"
                     /translation="LPSIAAVASCVPKGGHLDYEPKVPHFYTIVAIYSVVVVGAGLLA
                     KNRLLKTFSGLLGIGVVATYFAAEAALMSVWCFFSAILSTILYFYFHSKNRAMGMDSG
                     DGSSANYKAAA"
     gene            563..880
                     /locus_tag="PLH57_07745"
     CDS             563..880
                     /locus_tag="PLH57_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK02544.1"
                     /translation="MGQFYLSPATLSPVEILLDLAHQLTLVARANEKELARIISNILN
                     NAKEADATEVRISVKQSRDVVEVVISDNGRGIPAEVLAKLGSKGATFGKSGGTGLGFV
                     PRH"
     gene            1052..>2433
                     /locus_tag="PLH57_07750"
     CDS             1052..>2433
                     /locus_tag="PLH57_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02545.1"
                     /translation="MRRFFSRFQSSTTVAYFLLLVAIVAYPLFKDIKSIITDFQNRNI
                     QIPITSQEWSVVYGDSEFAVCKASEGCKKDAVRELFQSPYHQADTEHKNRILGQRPPR
                     VTWLGTRIPVETLKRAAELGANQLVLGWALSRHQIWVDGQLYISGYGIDSRFPFVIAI
                     PHERIKKNVPLEIVISVENIYNGVRPDAFSRFHAAGFTTPKASNAILSRDAHYYYVRS
                     IVFGLVNLLLAIVFILCWAVVRDRRHYLYFGFFLLALAARYLQFHLGTNINSNLYHTF
                     QFLMIADFAELSTTILFVLSLSKVAPSLFKPIQVISVLVCLSFSLTSFPKLEMEVYRA
                     PFRSYGIPFVLLFLTLYLVFLFKRRRSVLANRTIFFAIIIIFLIRVTIFAIVAPYAPT
                     NNRDIAFLLLDSGLIFSMLFSAVLFYDFVITFKQSERAQVIAEISAQVYHDIKSPISA
                     LKMLNNDESIS"
BASE COUNT          634 a          514 c          530 g          755 t
ORIGIN      
        1 ctaccctcaa tcgccgcagt agccagttgc gtaccaaagg gcggacatct cgattacgaa
       61 ccaaaagttc cgcattttta tacgattgtc gcaatatact cagtggtagt tgtcggcgcg
      121 gggctgttgg caaaaaaccg ccttcttaaa actttttctg ggctcttggg cataggcgtg
      181 gtcgcaacct atttcgccgc cgaagcggca ttgatgtcgg tctggtgttt tttctctgcg
      241 atcttgagca caattttgta tttctatttt cactcaaaaa atcgcgcaat gggtatggac
      301 agcggtgacg gttcgtccgc taactacaaa gccgcagcct aagtagctga gaaccaccga
      361 gatcactatc aagcggccga gagcgcttcg agaaattttt cgggataagt catttctgga
      421 aaatgccccg cactcggata aacaatgatt ttaggattac ttaaatactt cgcaaacgaa
      481 tcgggcgcag ttagatcatg gcttcgatcg ttgccgcccc aaagaagagg cgagggtacc
      541 gcaattcgcc cttcagcctg aaatgggtca gttctatttg tcgcccgcga ccttaagccc
      601 cgttgagata ctattagatt tagcgcatca acttacgtta gtggcacgcg ctaatgaaaa
      661 agagttagct cgtataattt cgaatattct caacaatgcc aaggaagcag atgctacgga
      721 agttcgcatt agcgttaagc aatcccgtga tgtggtagag gtggtaatta gcgacaacgg
      781 ccgtggcatt cctgctgaag tgttagcgaa actcggctct aagggcgcta cgtttggtaa
      841 gtcaggcggt accggactag ggtttgtacc acgccattaa gcaactcaaa tcctggggcg
      901 gcgaactcaa aattcaaact tccgaagttg gcacgcgagt agagctaatt ttgccgggtg
      961 gttttcctgt agcaacctaa aaatgaatac tatatccaat tcttaaaagg taacatttaa
     1021 gttatcgtaa aaagatttta aggagctggt tgtgagacgt tttttttcac gatttcaatc
     1081 gtcaaccacc gttgcatatt ttctattgct cgtcgcgatt gtcgcctacc cactttttaa
     1141 agatatcaaa agtatcatta ccgattttca gaatagaaac attcaaatcc cgattacttc
     1201 gcaagaatgg agcgtcgttt acggtgactc agaatttgcg gtttgtaagg ctagtgaagg
     1261 ttgtaagaaa gacgccgtca gggaactttt ccagagtccc tatcatcaag ccgataccga
     1321 acacaaaaat agaatcttgg ggcaacgtcc accccgggta acttggcttg gaacacgtat
     1381 tccggttgaa acattgaaaa gagctgcaga gctaggtgca aatcaattgg ttttaggttg
     1441 ggcacttagt cgccaccaaa tctgggtcga tggccagctt tatattagcg gctacggcat
     1501 tgatagtcga ttcccgtttg ttatcgcgat cccgcatgaa cgaataaaaa aaaatgtacc
     1561 gctagaaatt gtgattagtg tagaaaatat ttataacggc gttcgtccag atgcgttttc
     1621 tcgatttcat gctgcggggt tcacgacccc gaaagcgtca aacgcgattc tttcgcgaga
     1681 tgctcactat tattatgtgc gctctattgt atttggttta gtcaatcttt tactcgctat
     1741 cgtatttatt ctgtgttggg cagttgtacg cgaccgcagg cattacttat attttggttt
     1801 ttttctattg gcgttggcag cacgatattt gcagtttcac ttaggtacga atattaattc
     1861 gaatctgtat cataccttcc agttcctaat gattgctgat tttgcagaat tatctacaac
     1921 gattttattt gtactttcct taagtaaagt tgcgccttca ttgtttaaac caattcaggt
     1981 aatttctgtc ctagtatgtt tgtcgtttag tttaacaagt tttcccaagt tggaaatgga
     2041 agtgtatcgg gcgccgtttc gttcctacgg tataccgttt gtactgcttt tccttacgct
     2101 ttacctagtg ttccttttca agcgcagacg atctgtatta gccaatcgca caattttctt
     2161 tgcgataatt attatatttt taattcgggt tacgatattc gcaattgttg ctccatatgc
     2221 gcctactaac aaccgagata ttgcattctt attgttagac agcgggctaa ttttttctat
     2281 gttgttttct gcagtattat tctatgattt cgttattact tttaaacaat ctgaacgagc
     2341 ccaagttatt gctgaaattt ctgcacaagt ttatcacgat atcaagtcac ctattagtgc
     2401 gttaaaaatg ttaaataacg atgagtccat ttc
//
LOCUS       DAOMXO010000353         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25036_length_2432_cov_4.651241, whole genome shotgun sequence.
ACCESSION   DAOMXO010000353 DAOMXO010000000
VERSION     DAOMXO010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25036_length_2432_cov_4.651241"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLH57_07755"
     CDS             <1..395
                     /locus_tag="PLH57_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02546.1"
                     /translation="ENQKMIEFNPRVRNDLQLSFEQFLATKILTDEDFTIQARTEVAS
                     RADALANQNLTETEAFQSQKRALRSRYEDNAIRGFYLKGTLRDTSKSVVQFLFDNTLV
                     DDVFESDEALAGVVLSTLQNFDESKIAG"
     gene            complement(527..2131)
                     /locus_tag="PLH57_07760"
     CDS             complement(527..2131)
                     /locus_tag="PLH57_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HRK02547.1"
                     /translation="MAKEPMSARLRMTWKKSCVATLVSATATLTVSSVASAQISTIID
                     LPKNAPLSEMIRQAARYLQSNDSFHSGRLMGGAQIASEKSDFVLKRATGDAISTTLQY
                     VPVFGGIEVLGRATRFHFDARGKMRFVNDAKAVNVNTTPALSQKDVLATLRDRYNTNP
                     VIETGPTLKIFQDFSGTHRLIYAITTKTTEDHRGQKIYLDAHTGAFIMEAPRTYDLHA
                     ATDTRTFRKMFGDLAAANAQVTGKHVVMSADTQKALSKTGRDGYPSGFDLDWYDTVYT
                     DDKGFDMDIADRAAVNALVNARRVYEYYKSTFGRSSYNNKDSLITSVVHVGRNMNNAM
                     WSDEIGSMLYGDGDERLFRDLTIGLDVAGHEMTHGVISTSANLEYAAESGALNESFAD
                     FFGKMIDYVPGDWDIGATIMAEGFRHKRRALRNMIHPEEFDQPGDNDSNLRESTRGPC
                     SPFNDMCGVHTNSGIPNKASALMVVALGKEKVENLYYQVLTKHLTASSDFSDMRKQTE
                     QACDIMYGKSSTECAAVTKAFDTVKM"
BASE COUNT          580 a          586 c          607 g          659 t
ORIGIN      
        1 ttgagaatca aaaaatgatc gaatttaacc cacgggttcg caatgatctc caattgagtt
       61 tcgaacagtt cttggcaact aagattctta ccgacgaaga tttcaccatt caggcgcgca
      121 ccgaagtagc tagtcgtgcc gacgcgttag caaaccaaaa tcttactgag accgaagcct
      181 ttcagagtca aaaacgggct ttgcgcagtc gctatgaaga caacgcaatt cggggttttt
      241 atttgaaggg cactttgcgt gatacttcca agtcggtggt tcaatttttg ttcgacaata
      301 ccttggtaga cgacgtgttt gagtcagacg aagcgctcgc aggtgtcgtg ctgtccacgc
      361 tccaaaattt cgacgaatcc aaaattgcag gttgaagtca tcttagaaac acaaaacccc
      421 cgcgccgtac taactttttg gggaggcgcg agggttgtat gtgggtctgg ggtttctaaa
      481 cacgcttttg ggggaacgcg tctagaaact ttgatgcggt gtttttttac attttcaccg
      541 tatcaaaggc ttttgtcaca gcagcacatt cagtagacga tttgccatac atgatgtcgc
      601 aagcctgttc ggtttgctta cgcatgtctg aaaaatcaga actcgcagtt aagtgcttgg
      661 tgagaacttg gtaataaaga ttttcgacct tctccttacc gagagcaacg accatcagcg
      721 ccgaagcttt gtttggaatg ccactgttag tgtggactcc gcacatgtcg ttgaatggtg
      781 aacacggccc acgtgtgctt tcacgtaggt tgctgtcatt gtcgcccggt tggtcgaact
      841 cttctgggtg aatcatgtta cgaagggcgc gtcgtttgtg acgaaatcct tcggccatga
      901 tcgtggcgcc gatgtcccag tctcccggta catagtcgat catctttccg aaaaaatcgg
      961 caaacgactc gtttagtgcg cccgactcgg ctgcgtattc gagattggcg ctagtcgaaa
     1021 tcactccgtg agtcatttcg tgtcccgcaa catcgagccc aatagttaag tcgcgaaaca
     1081 aacgctcgtc gccgtcacca taaagcattg aaccgatttc gtcggaccac atagcgttgt
     1141 tcatgttgcg acccacgtgt acgacgctag tgatgagtga gtccttgtta ttgtaggaac
     1201 tccgaccaaa cgttgacttg tagtattcgt aaacacgacg ggcattgacg agcgcattca
     1261 ctgccgcacg gtcagcgatg tccatgtcaa agcctttgtc gtcagtgtac acggtgtcgt
     1321 accagtcgag atcaaacccg cttggatagc cgtcacgtcc agttttcgag agcgcttttt
     1381 gagtgtcggc actcatcact acgtgtttgc cagtgacttg ggcattggcc gccgcgagat
     1441 cgccaaacat tttacgaaag gtgcgagtgt cggtggcagc gtgcagatca taagttcgcg
     1501 gcgcttccat aatgaatgca ccggtgtgtg catccaaata tattttctga ccgcggtgat
     1561 cttcggtggt tttcgtggtg atcgcgtaaa ttaagcgatg ggttccactg aagtcttgga
     1621 atatcttaag cgttggtcct gtttcgatta ctggattggt gttgtaccga tcgcgtaatg
     1681 tagccagtac gtctttttga gacaacgcag gagtcgtatt cacgttgact gctttagcgt
     1741 cgtttacaaa ccgcattttc ccgcgcgcat cgaaatgaaa acgcgtggcg cgaccaagta
     1801 cttcgatgcc accaaatact ggcacatatt gaagtgtcgt cgaaattgcg tcgccagtgg
     1861 cgcgctttaa gacgaagtcg cttttctcag aagcgatttg agcaccaccc atgagtcgcc
     1921 ccgaatgaaa cgaatcgttc gactgcaaat agcgcgcagc ttgccggatc atctccgaga
     1981 gcggtgcgtt ttttggcaga tcgatgatgg tggatatttg agcagatgca acggacgaaa
     2041 cggtgagcgt cgcggtcgcg gaaacaagag ttgcaacaca actctttttc caagtcatcc
     2101 ttaaccgggc actcattggt tctttagcca tgaaccaacc tccttgttga agaagaaaaa
     2161 agtgctattc catgcgcttc ctctgcactc gccctaatgg ctttgtcaga agaagcgccc
     2221 ccttttttgc ttcaggtgtt caaatttctt cgaacccctt cggcttcgct ccgcagccga
     2281 caacccccat gttgtcattt ttcgggccct agagccgcgg agagtgtcat cattgacata
     2341 aacaagagta cgcaagtgca gtgccagctc aaaaatgtgc aggtcgtcaa tcgtttgttt
     2401 ttaaatttca atgagttagt ttttgattaa aa
//
LOCUS       DAOMXO010000354         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25037_length_2432_cov_4.353807, whole genome shotgun sequence.
ACCESSION   DAOMXO010000354 DAOMXO010000000
VERSION     DAOMXO010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25037_length_2432_cov_4.353807"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="PLH57_07765"
     CDS             complement(<1..165)
                     /locus_tag="PLH57_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRK02548.1"
                     /translation="MGKVIGIDLGTTNSCVAVVEGGEPKVIPNSEGLNTTPSIVAFMD
                     NGEKLVGQAAK"
     gene            complement(224..754)
                     /locus_tag="PLH57_07770"
     CDS             complement(224..754)
                     /locus_tag="PLH57_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRK02549.1"
                     /translation="MNTENPESTKPAASASEELENATDSAASGEYKLAELEAQLADQK
                     SKYLYLYAEFETYKKRAIKERSELLKFGHENFAGELLLTADNLERAIQNSTEAPKNLL
                     DGLKMVQQQLQDTFSRFGVTEIQSIEAKFNPELHEAVGQELSENKESGSILAVVQKGY
                     LLHGRLLRAARVIIAK"
     gene            complement(854..1963)
                     /gene="hemW"
                     /locus_tag="PLH57_07775"
     CDS             complement(854..1963)
                     /gene="hemW"
                     /locus_tag="PLH57_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRK02550.1"
                     /translation="MKFRNLYVHTPFCETKCHYCDFFSLPEGKYDSQSQIKVYRNILD
                     EFRFYRLGELETIFLGGGTPSLAPESFLEALFGKLDLDSNCEVTLEANPSSVTPTNAS
                     HWKSLGVNRVSLGVQALSNERLEWLGRVHSKAAAYKALEAVHNANIERVSIDYIVGVP
                     EQSLSQIESELGELLSKFPAVRHVSAYLLTLQTTNPKLAQLPNEEAQLQHLRMVRDVL
                     AAHGLLQYEISNFAEPGFEARHNLNYWNGGSYLGIGPSAHSFDSSEQLRWKNKASLSS
                     YCEDVENALPPIDWKESLDREQRRLEYLMLRLRTSAGIVTHDYSARFGRDLAADNDTR
                     ILQWEAAGLVSRSQDQIRLMGDGYFLSDSIIGAIR"
     gene            2055..>2432
                     /locus_tag="PLH57_07780"
     CDS             2055..>2432
                     /locus_tag="PLH57_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRK02551.1"
                     /translation="MFEKTYENGFELVVVPTQSPVAAVQVWVDVGSIDEAPKEAGYCH
                     FLEHMLFKGTKKRTTQEIAGTVEGSGGEMNAYTSFEHTVYHITLAADRWAIANDILFD
                     MALSSQFLPKEFAPEKEVILEEIK"
BASE COUNT          627 a          552 c          587 g          666 t
ORIGIN      
        1 cttagcagct tgtccaacga gcttttcgcc gttgtccata aaagcgacga ttgacggagt
       61 agtgttcaaa ccttccgagt tcggaataac tttaggttct ccaccttcga cgactgcgac
      121 gcaggagttt gttgttccca ggtcgattcc tattactttt cccataaatt cctccgttat
      181 aaagtttcaa aagttcctta actataaagc tgggactaaa atcttatttg gcaataatca
      241 cccgtgcagc tcgcagcagt cgaccgtgca aaagatagcc cttttgaact accgccaaaa
      301 tagagcccga ttccttgttc tcagaaagtt cctgaccaac ggcttcgtgc agctctgggt
      361 tgaatttcgc ctcaatagac tgaatttccg taacgccaaa acgactaaac gtatcttgca
      421 gctgttgctg gaccattttt agtccatcaa gaagattttt cggtgcttcg gtcgagtttt
      481 gaattgcgcg ctctaggttg tcagctgtca aaagtagctc accagcaaag ttttcatgac
      541 caaacttcag tagttcagag cgctctttaa ttgccctttt tttataggtt tcaaattcgg
      601 cgtaaagata gagatatttg cttttttgat cagcaagctg cgcctcaagt tcagctaatt
      661 tatattcgcc cgacgcagcg gagtcggtgg cgttctcgag ctcctcggaa gcagaagccg
      721 cgggtttggt cgattcaggg ttttctgtat tcaaattgtg ctccataata gtgggtcctt
      781 ccaaattaag atgggattac gcgtaaaaat gtcaagtcaa aaaagtgata actaattgta
      841 attattgtgt aatttatcta atcgccccaa taatggagtc gctcagaaag tacccgtcgc
      901 ccattagtcg aatttggtcc tgggaccgag aaacgagccc cgcagcctcc cattggagga
      961 ttcgagtgtc gttgtccgct gcgagatcac gcccgaaccg agcggagtag tcatgggtaa
     1021 ctattccggc actggttctg agtctgagca tcaagtactc gagacgtcgt tgttcgcgat
     1081 ccagcgactc cttccagtcg atcggaggaa gagcattttc gacgtcttca caatagctac
     1141 ttaaagaggc cttgtttttc caacgcaatt gttcggaaga gtcgaaagag tgagcgcttg
     1201 gtccgatgcc gaggtagctg ccaccattcc agtaatttaa gttgtgccgg gcctcaaaac
     1261 ctggctctgc gaagttagaa atttcgtact gtaacaaccc gtgagcggcg aggacatcgc
     1321 gtaccattcg caggtgttga agttgagctt cctcatttgg cagctgcgcc aatttgggat
     1381 tggttgtttg aagggttagt aaataggcgg atacgtggcg aacggcgggg aatttactga
     1441 gaagctcccc taattctgat tctatttgag aaaggctctg ttccggtact ccgacgatat
     1501 agtcaatgct tacccgctca atgtttgcat tatgaactgc ttcaagagcc ttataggccg
     1561 cagccttcga atgcacccgc cccagccatt ctagtctttc gttcgagagc gcttgtaccc
     1621 cgaggcttac acgattaacg cctaacgatt tccagtgtga agcgtttgtt ggggtgacgc
     1681 tagaggggtt tgcctctaat gtgacttcgc agtttgagtc taggtccaat tttccgaaaa
     1741 gcgcttccaa aaaagactca ggcgcgagag atggggtgcc accacctaaa aaaatggttt
     1801 caagctcgcc taagcggtaa aatctaaatt cgtctaaaat atttcgataa acttttattt
     1861 gggactgaga atcgtattta ccttcgggca atgaaaagaa atcgcaatag tggcatttgg
     1921 tttcacagaa cggagtatga acgtaaagat ttcggaactt cataaaaaaa tgtaggctgt
     1981 gaaagttagc cctgactcca ggggcatttc actaacataa aatcggtctt tatcccagcg
     2041 atgaggagct tacgatgttt gaaaaaactt atgaaaatgg ctttgaatta gtcgttgtgc
     2101 ccactcagtc gccggtagct gcggtacagg tttgggtcga cgtcggtagc attgatgagg
     2161 cgccgaaaga ggccggctac tgccacttcc tcgaacacat gctttttaag ggtacgaaaa
     2221 aacgtacaac ccaggaaatc gcgggcacag tggagggcag cggcggggag atgaacgcct
     2281 atacttcgtt cgaacacacg gtttatcata taacgcttgc cgccgatcgt tgggcgattg
     2341 ctaacgatat tctattcgac atggcgttga gcagccagtt tttgcccaag gaatttgctc
     2401 ccgaaaaaga agtcattctt gaagagatta aa
//
LOCUS       DAOMXO010000355         2430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25075_length_2430_cov_4.922947, whole genome shotgun sequence.
ACCESSION   DAOMXO010000355 DAOMXO010000000
VERSION     DAOMXO010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25075_length_2430_cov_4.922947"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..942)
                     /locus_tag="PLH57_07785"
     CDS             complement(28..942)
                     /locus_tag="PLH57_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRK02552.1"
                     /translation="MAPNDSTYLSISLATLRANIKLGFDVFIHISEKHILYIRGADSI
                     ENERLERLASKNVRHLYILDKDREAYEVFLKASQRAALSDPHMPLAEKAEVINGQAKS
                     AVEKMFENPESKENFEKAEQSARAQVELLKKVPAALETMMKIAEHDKTTYQHSVNVSN
                     LAISLAAKLGAAPEVCEIVGTGALLHDIGRGALTQGDMTADKYSQHPRAGAQMLKGKP
                     YITKDVLDIILLHEERLDGKGYPGGLKKIEQLFQIVGLCNLYDRHVTLMGEPPDQAFA
                     KISAMNPPPYQKELILALKDVLAKHKIL"
     gene            complement(948..1541)
                     /locus_tag="PLH57_07790"
     CDS             complement(948..1541)
                     /locus_tag="PLH57_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRK02553.1"
                     /translation="MKYNFLLDEEGYVRELRTALDGPSKSQSIEEKIRDGSLFRVTHT
                     LKGLSLMIGINSLVPSLTDLERAIAEGNSNAIEMRIRDFLDQFENEAMAHSAVRASKK
                     NEKNSGSQSSLLRLLFNGGESVDLLVWEIEQVLYAPRLISCGKRCYVYYRRSLVPVAR
                     GTHGKWVVVRAVGNGLDALICEAVDLVPVTTQQLKAA"
     gene            complement(1543..1899)
                     /locus_tag="PLH57_07795"
     CDS             complement(1543..1899)
                     /locus_tag="PLH57_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02554.1"
                     /translation="MKRYICFNANGNSYGVATDDLAGVSELSPMASYPGLPSGVSEIA
                     YWKGKLYVVPESDFFSPQAGGAVYLFTHRSAPAYALDLAFKISGNISVKRNDGSFTVL
                     SLDDLQRLRQRGQRAA"
     gene            complement(1968..>2430)
                     /locus_tag="PLH57_07800"
     CDS             complement(1968..>2430)
                     /locus_tag="PLH57_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02555.1"
                     /translation="RPVKAMILWGLVFDTWFTLKFVFMSTFYFFKTRFIYSPKRRSRL
                     TVTAQILKQESTTFLQDLEADARRLLDEQPHVHTVIMGHTHGPMHKVYPDGKVYINTG
                     TWTRMISLDLRGIGSNYRLTFAMIEYDDQGKAFASLQQWDGEHRPHRPFLS"
BASE COUNT          616 a          607 c          563 g          644 t
ORIGIN      
        1 ctcctaaatt accaagcggg agcgaattta caagattttg tgtttggcga gcacgtcttt
       61 cagcgcaaga atcagttctt tttggtaggg tggcggattc attgccgaga tttttgcgaa
      121 cgcctggtca gggggctcgc ccatgagggt cacgtgacgg tcatacaaat tacatagccc
      181 tacaatctga aataactgct cgatcttttt taggccaccg ggataccctt ttccatctaa
      241 acgttcttcg tggagcagaa taatatccag cacgtctttc gtgatgtatg gttttccctt
      301 gagcatttgg gcgcccgcgc ggggatgttg agaatatttg tctgccgtca tgtcgccctg
      361 agtaagggcg cccctaccga tgtcgtgcaa taaagcgccg gtccctacga tttcacaaac
      421 ctcgggtgct gctccaagct ttgccgccaa actgatcgcc aaattcgaga cattcaccga
      481 gtgttggtag gtggttttgt cgtgttcggc tattttcatc atggtttcta acgcagccgg
      541 cacctttttt aagagctcga cctgggcccg agcactctgt tcggcttttt caaaattttc
      601 tttggactcg ggattctcga acattttttc aacggccgac tttgcctggc cgttgatcac
      661 ttcggccttc tcggccaaag gcatatgggg gtccgaaagc gccgcacgtt gcgaggcttt
      721 tagaaaaact tcataagctt cccgatcttt atctaaaata taaagatgac gaacattttt
      781 agaggccagc cgctccagac gctcgttttc aatcgaatcg gcgccccgaa tatacaagat
      841 atgcttttca gaaatatgaa tgaagacatc aaacccgagt ttaatgttcg cgcgaagggt
      901 agcgagcgaa attgaaagat aagtgctgtc gtttggtgcc atgtcgccta ggctgccttt
      961 aactgttgag tcgtaaccgg aacaagatca actgcttcac aaatcaatgc atcgagtcca
     1021 ttacccaccg cacgaaccac cacccacttt ccgtgagtgc ctcgcgcaac tggcaccaga
     1081 ctccttcgat agtaaacgta acatcgcttt ccgcaagaga tcaaacgggg ggcatacaat
     1141 acttgttcga tttcccaaac cagtagatca acagattccc ctccattgaa aagcaagcga
     1201 agtagtgaac tttgactccc tgaatttttc tcgttttttt ttgacgcacg aacggctgaa
     1261 tgggccatcg cttcattttc gaattggtct aggaagtctc gaattcgcat ttcaatcgca
     1321 ttagaatttc cctcggctat ggctcgctcc agatccgtca agctcggaac gagcgagttg
     1381 atgccaatca ttaaagagag tcctttaagc gtatgagtca cgcgaaaaag cgatccgtca
     1441 cgaatttttt cttcaatact ctggcttttc gaagggccat caagggccgt gcgtagctct
     1501 cgtacataac cttcttcgtc gagtaaaaag ttatacttca tttcaggctg cacgctgccc
     1561 tcgctgacga agtctttgca aatcgtcgag ggagagcaca gtaaaagaac cgtcgtttct
     1621 ttttaccgaa atattaccgc taattttgaa tgcgagatcg agcgcgtagg ctggcgcgct
     1681 tctatgggta aacagataaa ccgcgccacc cgcttgcggc gagaaaaaat cagactcagg
     1741 cactacgtaa agcttgcctt tccaatatgc gatttccgaa accccgctgg gcaaaccggg
     1801 ataagacgcc attggactaa gttcagaaac gcccgccaag tcgtcagtcg cgaccccata
     1861 gctatttccg ttggcattaa aacaaatata acgcttcacc gagccacacc ccttcaatag
     1921 ggaatcggta tttcgataaa cgatctagag agcggtattt tgtgccacta actaagaaac
     1981 ggacgatgag ggcgatgctc cccgtcccac tgctgaagtg aagcgaaggc tttaccttgg
     2041 tcgtcgtatt cgatcattgc gaaggtaagg cggtaattac tgccgatccc ccttagatcg
     2101 agactgatca ttcgcgtcca cgtacccgta ttaatatata ccttcccgtc aggataaact
     2161 ttgtgcattg ggccgtgggt gtgccccatg attacggtgt gcacatgagg ctgttcgtct
     2221 aaaagacgtc tggcatcggc ttctaggtcc tgtaaaaaag tcgtactctc ttgttttaga
     2281 atttgtgcag tcactgttag acgacttcga cgtttgggac tgtagatgaa acgtgttttg
     2341 aaaaaataga acgtcgacat aaaaacaaac ttcaaggtaa accaggtgtc gaaaaccagg
     2401 ccccataaga tcatcgcctt aacaggtcgc
//
LOCUS       DAOMXO010000356         2423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25166_length_2423_cov_5.421875, whole genome shotgun sequence.
ACCESSION   DAOMXO010000356 DAOMXO010000000
VERSION     DAOMXO010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25166_length_2423_cov_5.421875"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1163)
                     /locus_tag="PLH57_07805"
     CDS             complement(174..1163)
                     /locus_tag="PLH57_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRK02556.1"
                     /translation="MSVDVNSLTEKVKVHSKFIDNVVSEISQVVVGQQSMVERILMAL
                     LCDGHILLEGLPGLAKTLTIKTMADTIHCGFNRIQFTPDLLPADLIGTVIYNQKTGEF
                     TPKLGPIHSNIILADEINRAPAKVQSALLEAMAEKQVTIAEQTYKLKDPFIVLATQNP
                     IEQEGTYPLPEAQLDRFMFKVKVGYPSVTEEKTILNRMTGTEGPRANRVIESGVIVEA
                     RQVVSSIYMDERIKDYILSIVFASRYPEKYGLSSLKGAIQVGASPRATINLAKGARAS
                     AFLSHRGFVTPEDIKTVAMDVLRHRIQVTFEAEADNVNSETVVRQILDRIEVP"
     gene            complement(1194..>2423)
                     /locus_tag="PLH57_07810"
     CDS             complement(1194..>2423)
                     /locus_tag="PLH57_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02557.1"
                     /translation="PLKPEVRRRLFNLENVAFVVKHLNQSEETITAPDGRRLVRNDLG
                     VLRDLFKMLNSSMPVDQRLNYNREHALSVVTSLTEFGLLRMIGRNIWVYDENGRKIAV
                     GLNSILKTLRDVAVQKTAVGRETNKDTLALLDHLLRKDVKPGVDPARATFDERYMFIG
                     KFIDQYFAWVQNDQCSAPPAHAAFKCSTAAIKSATYGLASFSADANNPLDLSKVLRAV
                     ISNQYTTFLGRNLDVLEDLIATPKAAGFFSGLERAADNENNRAQAYSDLLNITRTSLK
                     DMALSNAINTHHAVSIVKDLKEDAQNRWDKAVATLDSMKADPAYQRHREEVLEPIMKS
                     VVHWLPEKTALRPRLQTYLANHLRNGKTREIVRFGAQECVDDRSYSKIKFIGEPTYVS
                     GYQDFLEDLRAGLQDIH"
BASE COUNT          601 a          589 c          553 g          680 t
ORIGIN      
        1 ccaatcgata tggcgaatat cgtcgcccgg gtcgtattcc cgaaaatcgg aaaactgcat
       61 tccctggcct ttaaatcgcg tgtgatattg gccagaaagt acattgtcta cgactttgcg
      121 cgtggaaatt tctatgcgcc gcacacgacg caaaatttct tctgctcgct ctttcacggc
      181 acctctattc ggtccaggat ttgacgcact accgtctcgg agtttacgtt gtcggcttca
      241 gcctcaaaag taacctgaat gcggtgtcgg agaacgtcca ttgctacggt ctttatgtcc
      301 tccggagtta caaatcctcg atgcgacaaa aaggctgaag ctcgcgcacc ttttgccaag
      361 ttaatcgtag cccgcgggct ggctcccacc tgaatggctc cctttaaaga actgagccca
      421 tatttttcgg gatatcgact cgcgaacaca atcgatagaa tgtagtcttt aattcgttcg
      481 tccatgtata tcgaagaaac aacctgccgc gcctcaacta tcactcccga ctctatgacg
      541 cgattcgccc ttggtccctc agtgccggtc atgcgattca aaattgtttt ttcttcggtc
      601 acagatgggt acccaacttt taccttaaac atgaaacggt cgagctgagc ttcaggcagc
      661 ggataggtgc cctcttgctc aattggattt tgcgtcgcca acacaatgaa tggatctttc
      721 aatttgtagg tttgctctgc aatcgtcact tgtttttcgg ccattgcttc gagtaacgcg
      781 ctttgcactt tggcgggggc ccgattaatt tcgtcagcca aaataatgtt ggaatgtatg
      841 ggaccgagtt tcggagtgaa ctcgccagtt ttttggttat agataacagt tcctattaaa
      901 tctgccggta aaaggtccgg cgtaaactgt atgcggttaa atccacaatg aatcgtatcc
      961 gccatggttt taatcgtcag ggttttcgcc aatccaggca aaccttcgag caaaatatga
     1021 ccgtcgcata aaagagccat aagaattcgc tcgaccattg actgctgacc gacaacaacc
     1081 tgtgaaatct cgctaacgac gttgtcgatg aattttgaat gaactttgac cttctcggtc
     1141 aaggagttga cgtctacgct cataaattgt ccttttgaaa gcttgaatgt gaatcagtga
     1201 atatcttgca agccggcgcg caaatcttca aggaaatcct ggtaccctga cacgtaggtt
     1261 ggttcgccga taaacttaat cttagagtac gagcgatcat ctacgcactc ctgagcgcca
     1321 aaccggacaa tctctcgtgt ttttccattt cgcaaatgat tcgccaagta ggtttgcagt
     1381 cggggtctca atgcggtttt ctccggtagc caatggacta ctgacttcat gatcggctca
     1441 agaacttctt ctcggtgccg ttggtatgct gggtcggctt tcattgaatc aagggtagcc
     1501 actgctttat cccatcgatt ttgagcgtct tctttaaggt ccttaacgat cgacaccgcg
     1561 tggtgggtat taatcgcatt ggagagcgcc atatctttaa gagaggttcg agtaatattt
     1621 aacaaatcgg aataggcctg ggctcgattg ttttcattat ctgccgcgcg ttcaaggcct
     1681 gaaaagaaac cagcggcctt tggcgtagcg ataagatcct cgagcacatc gagatttctt
     1741 ccaagaaacg tagtgtattg gttcgaaatt accgccctta gtacctttga aagatctagc
     1801 ggattattgg catcggctga aaacgacgcc aggccataag tggcgctttt tattgcggcc
     1861 gtggaacatt taaaagccgc gtgcgctggg ggagcagaac actgatcatt ttgcacccac
     1921 gcaaaatact gatcaatgaa tttaccaata aacatatacc gttcgtcaaa tgttgcgcgt
     1981 gcggggtcta cgcccggctt cacatctttg cgtaacaaat gatccagcag tgccaatgtg
     2041 tctttattgg tttcacgtcc tacagcagtt ttctggactg ccacatcacg caaggttttg
     2101 agaatcgagt tcaggccaac ggcgattttt cgaccgtttt cgtcgtaaac ccatatgttt
     2161 cgaccaatca ttcgaagtag cccaaattcc gtcaaagaag tgaccaccga aagtgcgtgc
     2221 tctcgattgt aattcaagcg ttgatcgacc ggcatagagc tattgagcat tttaaaaaga
     2281 tcacgcaata cgccaagatc gtttctaaca agtcgccgcc cgtcgggcgc agttattgtt
     2341 tcctcggatt gattgagatg ttttacaacg aatgcaacat tttccaaatt aaagagtctt
     2401 cgccggacct ctggtttcaa tgg
//
LOCUS       DAOMXO010000357         2423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25169_length_2423_cov_4.380490, whole genome shotgun sequence.
ACCESSION   DAOMXO010000357 DAOMXO010000000
VERSION     DAOMXO010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25169_length_2423_cov_4.380490"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..790
                     /locus_tag="PLH57_07815"
     CDS             71..790
                     /locus_tag="PLH57_07815"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRK02558.1"
                     /translation="MATKNAIVLGGDGINCHRETAAACEMVGFSVDLLHVNDFLQSPE
                     RWLNRARLLVLPGGFSFGDELGSGRILALKLRGRAGSQLDRFLDNGGAMLGICNGFQV
                     LVAMEIFGRAVALAHNANGRFIDRWVTLNITGESIFTKHLPRTTRLELPIRHGEGRLV
                     CTDDAAWSTFRDSQALVLSYASDVNGSRDQVAGLSAREGRVLGLMPHPEAFWSSELHP
                     ASKDEKLTLGTELFAGAFKQL"
     gene            828..2420
                     /gene="purH"
                     /locus_tag="PLH57_07820"
     CDS             828..2420
                     /gene="purH"
                     /locus_tag="PLH57_07820"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRK02559.1"
                     /translation="MTKPQGLQSIRRALLSVSDKSLIEELARALVSAGAELLATGGTA
                     ERLHRANIPFRKVEDFTGNPEAFDGRMKTLSFKLESAILFDRAKSSHLEQAKQHDIEP
                     IDLVVCNFYPFENAVQREATEEELVSEIDVGGPTMIRAAAKNFKSVAALVDPEDYGLL
                     MAELRQYSGSTTLELRKQLMVRAFEYTARYDQIVYEQFAQKKLRYGENPHQAAYFVPS
                     RIKGAIDWELLDRSETELSYNNYLDLQAAFSVVRDARKLWTHGNVHSAPNSSGAVGVC
                     AIVKHNNPCGFATGSTTQEAFERAWAGDPVSSFGGVVVLSSQFTLECARLFDQKFVEV
                     IAAPDFEEKAIEELRRKKKKLRLLKIRTFDALATSDRFQRIAVEGGTLVQERDGGYER
                     EEFRSVTQKTFPRELEQLARFGIIASTHVKSNAVVIVRQTEDGGMQLVATGGGQPNRI
                     DAITSLAIPRAKAIVGRDLSSCVLVSDAFFPFADSVLAAADAGLRWVIQPGGSVKDSE
                     VIAAADERGLGMAFTGRRHFRH"
BASE COUNT          574 a          573 c          689 g          587 t
ORIGIN      
        1 accaccgaca ccagtggcca aatcgctgtt gcgggtgaaa ccttcgtaat tcgggatttg
       61 gagcgcgcgt atggccacta aaaacgcgat agtactcgga ggtgacggaa tcaactgcca
      121 ccgcgaaacc gctgcagcct gcgagatggt tgggttttcc gtcgacttgt tgcacgtgaa
      181 tgactttctt caatcgccag agcggtggct aaatcgggca cgattgttgg ttttgccagg
      241 cggtttttca ttcggagatg aactcggcag cggccgcatt cttgctctca aattgcgtgg
      301 ccgggccggc tcacagctag atcgtttcct cgataacggc ggcgcaatgc tcggcatttg
      361 caatgggttt caggttttgg tcgcaatgga aatatttggg cgcgctgtgg cgttggctca
      421 taatgcgaac gggcgtttca ttgatcggtg ggtcacgctc aatattacgg gcgagagcat
      481 cttcacgaaa cacctgcctc gcaccacgcg tttggaactt ccgattcgcc acggcgaggg
      541 gcgccttgtt tgcaccgacg acgcggcatg gagcactttc cgggactcac aagcacttgt
      601 tcttagttat gcgagtgatg tgaacggttc gcgcgatcaa gtggcaggcc tcagtgcccg
      661 agagggccga gtattggggc tcatgcccca ccccgaagca ttttggagta gcgaactaca
      721 cccagcgtca aaagacgaaa agttaacctt gggcactgag ttatttgcag gtgcctttaa
      781 gcaactttga cgtggccgga ctcgagtgac tgcgctatga aagtattgtg actaagcctc
      841 aaggtcttca aagcattcgc cgagctttgt taagcgtcag tgacaagtcg ctgatcgaag
      901 agttagcgcg cgccttagtc agcgcggggg ccgagttgct cgccactggt ggaaccgccg
      961 aacgtctgca tcgagcaaat ataccgttcc gaaaagttga agatttcacg ggcaatccgg
     1021 aagcttttga tggtcggatg aagactctta gttttaaact tgagagtgca attttatttg
     1081 accgtgcgaa atctagtcac ctcgaacaag cgaaacaaca cgatattgag ccgatcgatt
     1141 tagtagtttg caacttttac ccttttgaga acgcggtgca gcgagaagcg acagaagaag
     1201 agcttgtttc cgagatcgac gtaggtggcc ccaccatgat tcgggccgca gctaaaaact
     1261 ttaagtccgt cgcagcgctc gttgatccgg aagactacgg gctgctcatg gcggaactac
     1321 gccaatacag cggttcgacc acgttggaac ttagaaaaca attgatggtc agagcgttcg
     1381 agtataccgc gcgttatgat cagattgttt acgaacagtt tgcgcagaaa aaactgcgat
     1441 atggtgagaa ccctcatcag gcggcatatt tcgtaccgag ccgaataaag ggcgcgattg
     1501 attgggaatt actcgatcgt tccgaaactg aactgtccta caataattac ctagaccttc
     1561 aggctgcttt ttcggttgtg cgggacgccc gcaaactctg gacccatggt aatgttcact
     1621 ccgcaccgaa ctcaagcgga gccgtgggtg tgtgtgcgat tgtaaaacac aataaccctt
     1681 gcggtttcgc aactggatcc actactcagg aggcatttga acgcgcctgg gccggggacc
     1741 ccgtgagtag ttttggcggc gtcgtagtgc tttcgtcgca attcactcta gagtgtgcgc
     1801 ggttatttga tcaaaagttt gttgaagtga ttgcagcgcc cgatttcgag gaaaaggcga
     1861 tcgaagaact gcggcgcaaa aagaaaaaat taagactact aaaaattcga acattcgacg
     1921 ctttggcaac cagtgatcgg tttcagcgta ttgcagttga gggtggcacc ttggtgcagg
     1981 aacgagacgg tggttatgaa cgtgaagagt ttcgttccgt aactcagaaa acgtttccac
     2041 gcgaattgga acagttggcg cggttcggaa taattgcatc gacgcacgtg aagtctaatg
     2101 cggtcgtcat tgtccggcaa accgaagacg gtggcatgca attggtagcg accggcggcg
     2161 ggcaaccaaa tcgaattgat gcgattactt cgcttgcgat accaagagcc aaagcaatcg
     2221 tgggtcggga tctgtcgtct tgcgtgttgg ttagcgatgc gttttttcct tttgccgata
     2281 gtgtattggc cgccgcagac gctggtctac gttgggttat tcaaccgggt ggtagcgtaa
     2341 aggactccga agtaattgct gcggccgatg aacgcggctt aggaatggct tttaccggac
     2401 gtaggcactt tcgacactga act
//
LOCUS       DAOMXO010000358         2420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25203_length_2420_cov_4.098097, whole genome shotgun sequence.
ACCESSION   DAOMXO010000358 DAOMXO010000000
VERSION     DAOMXO010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25203_length_2420_cov_4.098097"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..826)
                     /gene="lepB"
                     /locus_tag="PLH57_07825"
     CDS             complement(47..826)
                     /gene="lepB"
                     /locus_tag="PLH57_07825"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRK02560.1"
                     /translation="MSNQDQSSSAKPPSTGRAKINRQVIIENVKSIGVAVFLVLIIRS
                     SIIEAFKIPSGSMIPTLLVGDHIFVNKFAYGLKIPFSDFFTDRPIYVVDRDPPKRGDI
                     IVFKFPKDEAFYYIKRVIGVPGDKVRVRDKVVYVNDKPIERVPLDKPDVIASIEGKQY
                     DKTSLSLFEETNSTTGEKYKVLLDRNTYITESYGEMSIPEDHLFVMGDNRDFSYDSRF
                     WGLVPIKNVRGKALFVWLSLWLDFRESQYYFRPSRIGTSIY"
     gene            892..1971
                     /gene="lptG"
                     /locus_tag="PLH57_07830"
     CDS             892..1971
                     /gene="lptG"
                     /locus_tag="PLH57_07830"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HRK02561.1"
                     /translation="MRILDRYVFFEFIRNFAIALVALALVFFLADYLRGVWDEEISAF
                     VLLKYGLMNLPAIACQMAPPAAMIGTMVTLSLLNRKNELTAMHAAGVSLMHISVLIFG
                     AIFIASCLTLVVHDRVVPPLARARTAYYWKVIKNRPDFSMDIKTSKIWYRSKNYIYNL
                     RTYDKKSETIHGIGIYFFDSTFRLIQHVEAENAKFEEGQWDLRNGMLTIFPEDNRFPL
                     SKHFDQKKFTLPETPSDFLQIEKQVESLRLKELRRFIQRNQEAGLETREYEVDFHARI
                     AVSFISLVMGLLAIPYSVRPKRQGGLGKDLAVCVGWIFSYWLLFSISLSLGRSGALAP
                     WVSVWGPSALFFGAALTLVTRVRAT"
     gene            1996..>2420
                     /locus_tag="PLH57_07835"
     CDS             1996..>2420
                     /locus_tag="PLH57_07835"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018206 - peptidyl-methionine modification
                     [Evidence IEA];
                     GO_process: GO:0031365 - N-terminal protein amino acid
                     modification [Evidence IEA];
                     GO_process: GO:0043686 - co-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRK02562.1"
                     /translation="MAILPINTFPDAVLRQRAEEVADFDAALERLVSDMLDTMYDAPG
                     VGLAANQIGLLMRVAVIDVDYEIESDEENTDPAPRKYKGKNPRVFVNPVITKRTGATP
                     FKEGCLSVPGFYEDVKRAVEVHVTYQDIKGTTHTLHATG"
BASE COUNT          643 a          551 c          571 g          655 t
ORIGIN      
        1 gaaaaagagc tgcgcgcaat caagaaaaag tcggctgctt aaaaaatcag tagatcgaag
       61 tgccaattcg agacggtcta aagtaatact ggctttcacg aaaatcgagc cacagagata
      121 gccatacaaa caaggctttg ccgcgcacat ttttgatagg cacgagcccc caaaaccgag
      181 agtcataact aaagtctcgg ttatccccca taacaaaaag gtgatcctct ggaatactca
      241 tctcaccata ggactcagtg atataggtat ttcgatcgag taaaacctta tatttttcac
      301 ccgtcgtgga gttcgtttct tcaaaaagcg aaagcgaggt cttatcatac tgcttgcctt
      361 cgatcgaagc gattacgtct ggtttatcga gtggcactcg ctcgattggt ttgtcattta
      421 cgtaaaccac cttgtctctc actcgaactt tgtcaccggg aactccgata acgcgtttaa
      481 tgtaataaaa cgcttcgtcc ttaggaaact tgaagacaat gatgtcaccc cgcttcggag
      541 gatcgcgatc aacgacgtag attgggcgat cggtaaagaa atcgctaaac ggaattttca
      601 aaccgtaggc gaacttattt acgaagatat gatcacccac caacaaagtg ggaatcatgc
      661 ttccggaagg gatcttgaag gcttcgataa tgctcgaccg aataattaaa accaagaaaa
      721 ccgctacccc gatcgacttt acgttctcaa taataacttg acggtttatt ttggcgcgcc
      781 ctgtcgaggg cggtttcgcc gaggaagact ggtcttggtt tgacatactg gtccaaagat
      841 agctgaaggg tttttcaaaa gcaaacgatt ctgggttatg ctgggtatgt catgcgtatt
      901 ctcgatcgtt atgtcttctt cgaattcatt cggaactttg ctatcgcgct agtcgcgctg
      961 gcgttggtgt ttttcttggc agattaccta cggggtgtct gggacgagga aatctcggcc
     1021 tttgtgcttt tgaagtatgg gctcatgaat ttgcccgcga tagcctgcca aatggcgccg
     1081 cccgctgcca tgatcggaac catggtgacg ctctctttgt taaatcgaaa aaatgaactt
     1141 acggccatgc acgccgctgg ggtaagttta atgcacattt cggtgcttat ttttggtgcc
     1201 atttttatag cgtcttgtct cacattagtg gtgcacgacc gtgttgtccc tccactcgct
     1261 cgcgcccgca cagcctatta ttggaaggtt ataaaaaatc gtcccgattt ttcgatggat
     1321 attaaaacca gcaaaatttg gtatcgcagt aagaactaca tctacaatct gaggacttac
     1381 gataaaaaat cagaaacaat tcatggcatt ggtatttatt tttttgactc cacttttcgt
     1441 ttaattcaac acgtcgaagc agagaatgcg aagtttgaag aaggtcaatg ggatcttcgt
     1501 aatggcatgt taacgatatt tcccgaagac aaccgctttc cacttagtaa acattttgat
     1561 cagaaaaaat tcactctgcc cgaaacccca tcggactttc tccagatcga gaaacaagtg
     1621 gaatctttac ggctcaagga attgcgacgc tttattcaac gtaatcagga ggcgggctta
     1681 gaaacccgcg agtacgaagt agattttcac gcacgcattg cggtgtcttt tatatcactc
     1741 gtcatgggac tgcttgctat tccctattcc gttcgcccaa agcggcaagg cggtcttgga
     1801 aaggatctcg ccgtgtgcgt ggggtggatc ttttcttatt ggcttttatt tagcattagc
     1861 ttatctcttg ggcgatcagg agcattggcg ccatgggttt cggtttgggg gccatccgcg
     1921 ctcttttttg gcgcagcgtt gaccttggtc acgcgagttc gagcaacctg aacctcctcg
     1981 taaaaaggcg aaaagatggc gattttacca ataaacacct ttcccgatgc cgtacttaga
     2041 cagcgagctg aagaagttgc tgattttgac gcagcgctag agaggcttgt ttctgacatg
     2101 cttgacacca tgtacgatgc gccaggagtg gggttggctg cgaaccagat tgggctttta
     2161 atgcgggtag cagtaattga cgtcgactac gaaatcgaat cggacgaaga aaacacggac
     2221 cctgcgcctc gaaaatataa aggaaaaaat ccgcgagtgt ttgtgaaccc tgtaatcacc
     2281 aaacgcactg gagccacacc gtttaaagag ggttgccttt cagtgccggg tttttacgaa
     2341 gatgtaaaac gtgcagtcga ggtgcacgtc acctatcaag atatcaaagg cacgactcac
     2401 accttgcatg ctactggctt
//
LOCUS       DAOMXO010000359         2419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25217_length_2419_cov_4.611252, whole genome shotgun sequence.
ACCESSION   DAOMXO010000359 DAOMXO010000000
VERSION     DAOMXO010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25217_length_2419_cov_4.611252"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="PLH57_07840"
     CDS             <1..1493
                     /locus_tag="PLH57_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02563.1"
                     /translation="FEPYLVPSIDGAIVVHENGGGATNRFLPAGAAPAEIEAKTKEAV
                     EKIAAARSKLGAGFGASSAQDYKKRLLTDVRFRVDEWDRVFRAGLVKANPVKDGTVFK
                     SNKYSFQTITRIKDTYVRKFDTGRVEVYGNNGRLMQVFDRNKNFVRLIYNKSGQMTQL
                     VDNLNRKMTFSYNKNGKVEKILGENGKKVEYRYDGFVMVYRKNSDGQVYEYKYSNNGR
                     YNLLEVKFPDKSTMKIDYNDVAKGETVASLRDRDGTLTKYTYMNDGVNGLYRGTALTV
                     FSSDGKEISKAKYEYWDKNKLDGERYTYKMSTDVDGEKTETVYNECCGLPLVITRNGE
                     KTSFEYDARGHVTRKTTPFEVTELTYDPQTNKVAKVARFPMGEKDPKKVNWATYKYDS
                     KGNLVEARNSAGQGVKIAYNSGTGQIMAMVDQAKRRLEFAYNEANRPVEIRDPALGKI
                     KVQYSASGDVQKVDTVGDRAIAGQITSAFQNLMELIRPAGVSLTSK"
     gene            1579..1836
                     /locus_tag="PLH57_07845"
     CDS             1579..1836
                     /locus_tag="PLH57_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02564.1"
                     /translation="MKLLKLLPFLAILATSGLAFAEQDNCTTSPDDADQLTVRDLSGL
                     QVADAAAAPKHNNNDEITKRPEGSSGGPAKGNSKSITTDDN"
     gene            2030..2272
                     /locus_tag="PLH57_07850"
     CDS             2030..2272
                     /locus_tag="PLH57_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02565.1"
                     /translation="MKKRMLIRWFPGLLGLMGVLFAPVAGAALKVGEIKNTDDMLSHL
                     ECNRQEAREVPQSISDSQIRDQSSPTVGRTVAQRVE"
BASE COUNT          694 a          532 c          607 g          586 t
ORIGIN      
        1 aattcgagcc ttatttggtg ccgtcgattg acggtgctat tgtcgtacat gaaaacggtg
       61 gtggcgccac caatcggttt ttaccagctg gagctgcgcc cgctgaaatt gaagccaaaa
      121 ccaaagaagc agtagaaaaa attgcagcag ctcgttcgaa attaggagct ggattcggcg
      181 cctcaagtgc gcaggactat aaaaagcgac ttttaacgga tgtacgcttt cgcgtcgatg
      241 aatgggatcg agtttttcgt gctggcttag tgaaggccaa cccagttaaa gacggcacag
      301 tattcaaaag caacaaatac agttttcaaa cgattactcg aattaaagac acctatgtgc
      361 gtaaattcga taccggtcgc gtagaggttt acggcaacaa tggtcgtctc atgcaggtat
      421 ttgaccgcaa taagaacttc gtccgattaa tttataataa atccggtcag atgacccagc
      481 tcgtcgataa tcttaatcgc aagatgactt tttcctataa caaaaacgga aaggtcgaaa
      541 agatcctcgg tgaaaacgga aaaaaagtcg agtatcgcta cgacggtttc gtaatggtct
      601 atcgtaaaaa ttcagacggc caagtttacg aatataaata ttctaataat ggccgataca
      661 atttgctcga agtaaaattt cccgacaaaa gcaccatgaa gatcgactac aacgatgtag
      721 cgaaaggcga aaccgtcgcc agccttcgcg atcgtgatgg tactcttaca aagtacacct
      781 acatgaatga cggggtgaac ggcttgtacc gcggcacggc cttgacggtg ttctcgtctg
      841 acggcaaaga aatctctaaa gcaaaatacg aatattggga caaaaacaaa ctcgacggcg
      901 agcgatacac ttataaaatg agcaccgatg tcgacggtga aaagaccgaa acagtatata
      961 acgaatgctg cggtcttccc ttggtcatta cgcgcaatgg tgaaaaaacc agttttgagt
     1021 atgacgcccg agggcatgtt actcgaaaaa ctactccgtt cgaagttacg gagctaacgt
     1081 acgatccaca gactaacaaa gtagccaaag ttgcccgttt cccgatgggc gagaaagacc
     1141 ccaaaaaggt gaattgggct acttacaaat acgatagtaa gggcaacttg gtagaagctc
     1201 gcaattcagc agggcagggt gtgaagatcg catacaatag cggcactggg cagatcatgg
     1261 cgatggtcga ccaagcgaag cggcgtttag agtttgctta caatgaagcc aaccgaccag
     1321 tcgaaattcg cgatccggcg ttagggaaaa tcaaagttca gtattctgcg tcgggcgatg
     1381 tgcaaaaagt tgataccgtt ggagaccggg cgattgccgg gcagatcact tcggcttttc
     1441 agaacttgat ggagctcatt cgaccagcgg gagtgagtct cacttctaag taaaatcaaa
     1501 ggtttataat tgttgatttt attaggtggt ttttagggtg agagctgata aaatattaga
     1561 agtgttgggg aaaattaaat gaagttattg aaattactgc cttttttagc gatcttggcg
     1621 acttcgggac ttgcgtttgc tgagcaggac aattgcacca cttcgcccga cgacgcggac
     1681 caattgaccg ttcgtgacct cagcggcttg caggtggcgg acgccgccgc tgctcccaaa
     1741 cacaataata acgacgagat cactaagagg ccagagggta gctctggcgg cccagctaag
     1801 ggcaactcga agtcgattac taccgacgat aattaagcca ctcggcactg tctaaatatt
     1861 agacactttt agcagctcct aacagcattc atcccttgtc ttagaagccg ggaccctgcg
     1921 tcaatctggc accccccttg caaactccta tctctgtaga gcaatagcgg aaattgagct
     1981 tcacccgttt tggaagctca tgtggatgtt gtagcagagg agagttgttg tgaagaagcg
     2041 gatgttgatt cgttggttcc ctggactttt gggtcttatg ggagttcttt ttgccccagt
     2101 tgccggagcg gccttaaaag taggcgaaat taaaaataca gacgacatgt tgtcgcacct
     2161 tgagtgcaat cgccaagagg cccgcgaggt accgcagtct ataagtgatt ctcagataag
     2221 agatcaatcg agtccgactg tgggtcgaac ggtagcacag agagtcgaat aagcgattag
     2281 gttaatcttc gttcgaagcc tcgtcttcat cgagactgtc ctctcgatca agatggggct
     2341 tcgctatttt cttaccggca ctttttaaaa gtccgctcaa ttcggcgcgc tccatgatgg
     2401 cgtgctcaga cgaaatgtt
//
LOCUS       DAOMXO010000360         2417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25250_length_2417_cov_4.719306, whole genome shotgun sequence.
ACCESSION   DAOMXO010000360 DAOMXO010000000
VERSION     DAOMXO010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25250_length_2417_cov_4.719306"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1539)
                     /locus_tag="PLH57_07855"
     CDS             complement(205..1539)
                     /locus_tag="PLH57_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02566.1"
                     /translation="MLFWFIAVLFPLAAAHGADPSCDVVLAAESRHLPENVRNLLKKP
                     RPEDTLLLPIPVARGASPVFIALDGANLTMTVSTEAALGQQLLPTHSHDAKLTANLIS
                     QFKSIRELPETHRPEALKAFAKSLEQISITTNLNFHSTRDNQITLEVATSDLLGHWLV
                     PFSTFLSQHSRSGNTVTRISPLDFKGSMNAHVLADVNQDDIIISYNSPEFLQWNRIWY
                     VTLHELFHWLQNQRKAAKLNYFPDLASSLLPSLHEGSAFVPRLEVRGTHAFHAKVSQQ
                     IKTYYDEHSLEEAGAFFFQSNMQRLQAQSFFSTDTVHSLDGQRYVTALLFRALEFYWY
                     SLSITRADLLAIKDYLKKNGDDRPDLMDRVVQARDFLRKRQAYQMSAAREFAKSFQEL
                     VHLDLTEVHYHGHPVLLRELVHQLSVFADNAPRIDTAGLHLIDGTSFEAPGK"
     gene            1620..>2417
                     /locus_tag="PLH57_07860"
     CDS             1620..>2417
                     /locus_tag="PLH57_07860"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRK02567.1"
                     /translation="MNAGLLKRLFSYLQSIHYVWGPLTPDRLIEARRNPAHFAIRAQL
                     LGLGLGIAQTSVEEIFPNELYAELQNAGFLFSKEANGESLVFSRYAVSGFSDLFVLHD
                     HWPPKVGLETSFVHVGHESTTLARIIQSELEAFISKSVLDLGCSSGILSLEVAGVAKR
                     VCGIDLCEPATRLATTTASAYGFLNTEFIAESVLDPNLLEHVRRAYWDRVIFNPPMVV
                     PAEVPYPHRDGGKYGIEIPLKFFDFALAALKLGGELFCLCTNPIVKGK"
BASE COUNT          609 a          536 c          629 g          643 t
ORIGIN      
        1 acccagcgaa tcagccttcg acatgagaag gtattgcacc atgaagataa ccagaatgct
       61 ccgcattccg taatagctga agcgctcaca ggcttcttgg ccaatgatat accgaatctg
      121 tggtggaaaa ccgactttgc ttgacatgta atgtccccct tagaggcgaa tagctcaaga
      181 actagggatt gccaatcagg gattctactt gccaggcgct tcgaaagaag ttccgtcgat
      241 caaatgcagg ccagcggtgt cgattcgagg ggcattgtcg gcgaaaacag acagttgatg
      301 gactagctct ctcagtaata ccggatgccc gtggtagtgc acctcggtca ggtcgaggtg
      361 cacgagctct tgaaagcttt tcgcgaattc gcgtgcggcc gacatttgat aggcctgacg
      421 tttgcgtaga aagtccctcg cctgaaccac tcgatccatt aaatctggcc gatcgtcgcc
      481 attttttttc aaataatcct taatggcgag taaatcggcc cgggtaattg agagcgaata
      541 ccaatagaac tcaagtgctc gaaacagcaa tgcagtcaca taacgttgtc catcgagcga
      601 atgtaccgtg tcggtggaaa aaaaagactg ggcctgtagg cgctgcatgt tggattgaaa
      661 aaagaatgcg cccgcttcct ccagggagtg ttcgtcgtag taggtcttta tttgttggct
      721 aactttagca tgaaaggcat gcgtgcctcg aacctcgagc cgaggaacga aagcgctgcc
      781 ttcgtgtaaa gatggcagca gcgatgaagc taaatcagga aagtaattca atttggcggc
      841 ctttcgttgg ttttgaagcc aatggaatag ttcgtgtagg gtgacgtacc agatacggtt
      901 ccactgcaaa aattcgggtg aattatagga aatgataata tcgtcctgat tcacatcggc
      961 aagtacgtga gcgttcatac tccccttaaa gtccagagga ctgattctcg ttacagtatt
     1021 tccgctgcga gagtgttggc ttaaaaaagt tgaaaaaggc accaaccaat ggccgagcaa
     1081 atctgaagtc gcaacttcta gggttatttg gttatctcgg gtgctatgga aattaaggtt
     1141 cgttgttata gagatttgtt ccaggctttt tgcgaatgcc ttcagggctt caggcctgtg
     1201 agtttccggt agttcgcgta tcgatttgaa ctgggatatt aagtttgccg taagtttagc
     1261 gtcgtgactg tgagtgggta gcagctgctg acctaaagca gcttcggtgc tgaccgtcat
     1321 ggtgaggttt gcgccgtcga gtgcaataaa aacaggggat gccccccgag cgacgggtat
     1381 gggcagtaac aaagtgtctt ccggtcgcgg cttttttagt aaatttcgta cgttttcagg
     1441 caggtgccgg ctttcggcgg ccagcacgac gtcgcaactt gggtctgctc cgtgggcggc
     1501 tgctagcgga aataaaactg ctatgaacca aaaaagcata gccccctata taatgaccga
     1561 ctaataatat caacaaatca gtgagcagct tttggaaaat taaatgataa catgatgtta
     1621 tgaacgctgg cctacttaaa agactcttct cttacctaca atccattcac tatgtttggg
     1681 gtccgcttac cccggaccgt ctgatagagg cgcgacgcaa ccctgctcat tttgcgatta
     1741 gggcccagct tttgggttta ggcctaggca tcgcgcaaac aagcgtggaa gaaatttttc
     1801 cgaatgagct ttatgcggaa cttcagaatg ccggcttttt atttagcaag gaagccaacg
     1861 gagaatcgct tgtattcagt cgctacgcgg taagcggttt ttcggatttg ttcgttttgc
     1921 atgatcattg gccgcctaaa gttggtcttg agacgagttt cgtgcacgta ggtcacgaat
     1981 cgaccactct cgcccgaatc attcagtcgg agttggaggc gtttatttca aagtccgttc
     2041 tcgacttagg atgtagcagc ggaattcttt cgttagaagt cgcgggagtg gcgaagagag
     2101 tgtgtggtat tgatctttgc gagccggcga ctcgattagc taccacgacc gcctctgctt
     2161 atggattttt aaatactgaa ttcatcgcag agtcagtgtt agatcctaat ttactggaac
     2221 atgttcgcag agcctactgg gaccgagtga tttttaatcc tccgatggta gtgcccgccg
     2281 aagtccccta ccctcatcgc gatggtggaa aatacggcat cgagatacca ttaaaatttt
     2341 ttgattttgc attggcggca cttaaactcg gcggtgaatt attctgcctg tgcaccaatc
     2401 cgatcgtaaa aggtaaa
//
LOCUS       DAOMXO010000361         2415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25283_length_2415_cov_5.870339, whole genome shotgun sequence.
ACCESSION   DAOMXO010000361 DAOMXO010000000
VERSION     DAOMXO010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25283_length_2415_cov_5.870339"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLH57_07865"
     CDS             complement(<1..723)
                     /locus_tag="PLH57_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRK02568.1"
                     /translation="MMKRSEAKKSKKAKSTRAQVPKVVTPEILEGELAEDEGRSTANS
                     EGAEGDEGEVLEAELVGDPGFPVPAGASGPDLAAELEQLPALPPHLDPFKHYLRDVSK
                     YPLLSPEDEFALAVKLRDTGDIDAAKRLVQANLRLVVKIAMEYRSAYQNILDLVQEGN
                     VGLMKAVSKYDPSKGARLGYYASWWIRSYILKYILDNFRQVRVGTTAAQKKLFFHLMR
                     EKERLEAQGLEAGPKLLAEKLNV"
     gene            1015..1932
                     /locus_tag="PLH57_07870"
     CDS             1015..1932
                     /locus_tag="PLH57_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02569.1"
                     /translation="MKGLFTRTSVIGAVALWSVAGLAASTSTTVSNAPASSGAVSETS
                     IQDVAAQPKALPISAAAVNDFYGPALNDLGARQTADSAKDAVQPLNSRHQIGISYAID
                     ESTKFTPTLDFSLNYTIPKGVENGERNFAWNDSYLKLSRAGLLDGKLGGNAVALDGDI
                     RYMLPTSKGARDADSYGAVRLSLSPSMQIANSNFSIVATNYARIHMYRSVTENLSATR
                     WKFYTGPQVNYAVNDKVTAFVLFEAVAAFDNQGYSNTTNDQLSLADLEPGVELNLHER
                     VSLTPFLNWYTNQPISTTSINLNATIKML"
     gene            2109..>2415
                     /locus_tag="PLH57_07875"
     CDS             2109..>2415
                     /locus_tag="PLH57_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02570.1"
                     /translation="MAQASLFFEHDGKLVGPYSVDEIAQQLHAGSLRVSARLFDADND
                     RWTTAADVIPNLNFESPRDWRPPARPVDLSNVHVAKLNRERTEGIDYFALISERKKEI
                     "
BASE COUNT          585 a          654 c          552 g          624 t
ORIGIN      
        1 cacgtttaat ttttctgcca ggagcttcgg cccagcctcg agcccttgcg cttcaagccg
       61 ttccttttcg cgcataaggt gaaaaaacag ttttttctgg gctgccgtgg ttccaacgcg
      121 cacttggcga aagttatcga gaatgtattt caaaatatag gagcgaatcc accaactcgc
      181 gtaatagccc agccgcgcgc ctttactggg atcatacttg ctcacagcct tcatgagccc
      241 tacgttgccc tcttgaacca gatctagaat gttctgataa gcggagcgat actccatggc
      301 aatcttcaca accagcctaa gattagcctg caccagtcgt tttgccgcat caatgtcccc
      361 agtatcgcgc aacttcaccg caagcgcgaa ttcgtcctcg ggcgaaagaa gtggatattt
      421 cgacacgtca cgaaggtaat gtttaaacgg atctaaatga ggaggaagcg ctggcaactg
      481 ctcgagttca gcggccaaat ccggccccga agcaccggct ggtaccggaa atccgggatc
      541 ccccaccaac tcggcctcaa gaacctcccc ttcgtcgccc tctgcccctt cggaattggc
      601 cgttgatcga ccttcgtcct cggcgagctc gccttcgagt atctcagggg tgacgacctt
      661 cggtacttgt gcccgagtag atttggcttt ttttgatttt ttggcttctg agcgtttcat
      721 catgatcggt gtccccaact ctataatacg aacccttgat tttcacggac cttttcaagt
      781 ataacgcggt gcgatttcgc cgaatggtgc aaagacgact cgtttgttag acatttctgt
      841 aaaaattaca ggaatccaag ggtctcagat gtccaaattc tgaacaacct ttgagtgcta
      901 aaaccttgtt ttacttctga aaaacgggct tttttcgcca tgaaattctg cttggtacac
      961 ggcttgcttt gtgcatcggc ggacttaaag ctttgttttt ttaacggagg tatcatgaaa
     1021 ggtcttttta ctcggacttc agtaatcgga gcagtagctc tgtggtcggt agctggtctt
     1081 gcagcttcga cttcaacaac ggtttcgaac gctcccgctt caagcggcgc tgtttcagaa
     1141 accagcattc aagacgtggc tgctcaaccg aaagcacttc caatctcggc cgctgctgta
     1201 aatgactttt acggcccagc cctgaacgat cttggtgctc gccaaactgc agattccgca
     1261 aaggacgcag ttcaaccact aaacagccgt caccaaatcg gcatttccta tgcgattgat
     1321 gagtcaacta agtttactcc aaccctcgac ttcagcctta actacacgat tcctaaaggt
     1381 gtagaaaatg gtgaacgtaa cttcgcatgg aatgacagtt acttgaagct tagccgcgct
     1441 ggcttgcttg acggcaaatt gggcggaaac gctgtcgctc tcgatggcga cattcgctac
     1501 atgcttccta cgagcaaagg tgctcgtgac gctgactcgt acggtgcagt tcgtctgtcg
     1561 ctttctccaa gcatgcagat cgcgaattcg aacttttcaa tcgtagctac aaactacgcg
     1621 cgcattcaca tgtaccgctc ggttacagaa aatctttcgg ctactcgctg gaagttctac
     1681 acgggcccac aagtgaatta cgctgtgaac gacaaagtta ctgcgtttgt tcttttcgaa
     1741 gccgtagctg cttttgacaa ccagggttac tccaacacca cgaacgatca actctcgctc
     1801 gcggacttgg aaccaggtgt tgaactcaac ttgcacgagc gcgtatcgct cacgccgttt
     1861 ctgaactggt acacgaacca gccaatcagc acgacgtcga tcaacctgaa cgctacgatc
     1921 aagatgctct aaaacggagc acgatctaag agttctaaga acttagaaat caaagccctc
     1981 tcaaacttcg gtttgagggg gctttttgct tttgcaggcg ctacaagttt gattttgccg
     2041 gtcggcaaaa ccttcttctg tttcaccaac attcccgccg atacaatatt ggtcggggaa
     2101 tggtgggaat ggctcaagct tcactctttt ttgaacacga tggcaaacta gttggcccat
     2161 acagcgtcga cgagattgcc caacaattgc acgccggctc acttcgagtt tccgctcgcc
     2221 tgttcgacgc cgacaatgac cgctggacca ctgcggcaga tgtaattccc aatctcaatt
     2281 ttgagtcccc tcgcgactgg cgcccaccgg ctcgaccggt agatcttagc aatgtgcacg
     2341 tggcgaaact taatcgtgaa cgcaccgaag gaatcgatta tttcgcatta atttccgaaa
     2401 gaaaaaaaga aattc
//
LOCUS       DAOMXO010000362         2406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25417_length_2406_cov_4.134411, whole genome shotgun sequence.
ACCESSION   DAOMXO010000362 DAOMXO010000000
VERSION     DAOMXO010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25417_length_2406_cov_4.134411"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1182)
                     /locus_tag="PLH57_07880"
     CDS             complement(58..1182)
                     /locus_tag="PLH57_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02571.1"
                     /translation="MSNENEKSDLLPDLEVHAIDEGFEIETTREATAAPSTKKTEPPK
                     EGTISDFAAPQTEFAAPLEGLDPQSESPIELGSAPEPDQAFSEVNEASAPASDQALSA
                     LQASAPETPLDAPPAGLDQPQHSPEADQSAKATLKSVGEFAGRMTIGHPTVEANPAFS
                     LFAKGTFNAEKIERIKGVLTSDEYGVRFEDVETQLSVGKLMVPRISEYAAIQLGQKIF
                     DLVDTVEIALSDEIVEGQVPDDELIDPDHFRKRTTDAHDLGDEPGSEADVFTATIGDF
                     AGFTITRILSAITLSEIVPASVAEAPQSHEFEQVTEKLTRELASRAFKLGAHGVIGIS
                     FAVKLIEGYRDLGGTDTRAYRMLATGTAVKAKRNLTPPGL"
     gene            complement(1190..>2406)
                     /locus_tag="PLH57_07885"
     CDS             complement(1190..>2406)
                     /locus_tag="PLH57_07885"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HRK02572.1"
                     /translation="SPLLALFTPIYRLWSEAYSLFLAQASLLGLSAVLVYKLARAKKV
                     PREWAVVLGSLFLIYQPLRMANIFDLREDNLFVPVFLGALLALERQRMTLFVLSCVAA
                     WFIKENAGIFTMLLGLWVAGRKKQRHVLVGVSLAVTSLIVLSVVNLYVTPYFSGAGPT
                     MLAKRFGLDAAGIVEASTVRPLQLLEEVWARMIQRENLRYVLKVLTPFVPFAWAAPIP
                     FLIACAGLAMNTALDLKFGFHYECVLIPFLFYVLISGVQTLLSGGVSFFSRRLNPGRP
                     ELLMTLVLLFMLFFGRSPAFTVREFRPDERDAFISKVLEQIPPNVSVTAQMIVHPHVC
                     TRTQADLFRGTPPTTDYVVFDLSPWRSQWGVNNLEKVAASLDPAAYQKVIDTDGFLIF
                     KKFAAPGLIDVQ"
BASE COUNT          618 a          612 c          658 g          518 t
ORIGIN      
        1 tgcaagaata tgaaacattt gaatacccca gaaaaacggt cctactatag cggggtgtca
       61 aagtccagga ggtgtgaggt tgcgcttggc tttaacggca gtgccagtgg ccagcatgcg
      121 gtaggcccga gtatcggtgc cgcccaggtc ccggtaccct tcgattaact ttacggcaaa
      181 cgaaatgccg atcacaccgt gagcccctag tttgaatgcg cggcttgcca gttctcgggt
      241 aagtttttcg gtaacttgtt cgaattcgtg ggactgaggc gcctcggcca ctgaggcagg
      301 aacaatttca ctcaaggtaa tagcagataa aatacgggtg atcgtaaaac cggcgaaatc
      361 gccgatcgta gccgtaaaaa cgtcggcctc ggaacccggt tcatcgccaa gatcgtgggc
      421 gtcagtggtg cgttttcgga agtggtcagg gtcgataagt tcgtcgtcgg gaacttggcc
      481 ctcgacgatt tcgtcggaca aggcgatttc tacggtatct accagatcga aaattttttg
      541 gcccaactga atggcggcgt attccgaaat acgagggacc atgagttttc ccactgaaag
      601 ctgagtttcg acgtcttcaa aacgcacgcc gtattcatcg gacgtcaaga cgccttttat
      661 tcgctcaatt ttttcggcgt taaaggtgcc ttttgcgaaa agagaaaaag ccgggttggc
      721 ttcgaccgtc ggatgcccaa tggtcatgcg gccggcaaat tccccaaccg actttagagt
      781 ggccttggct gactggtcag cttccggaga atgctgaggt tggtcgaggc cagccggtgg
      841 cgcatcgagc ggggtttcag gggcactcgc ctgcaacgcc gacaacgcct gatcactggc
      901 gggagcggac gcttcgttta cctctgaaaa agcttggtcc ggttcgggcg cagaccccag
      961 ttctatgggg gactcggact ggggatctaa gccctcgaga ggggcggcaa actcggtttg
     1021 aggtgcagcg aaatcgctta tcgttccctc tttggggggt tctgtttttt ttgtgctcgg
     1081 cgccgccgtg gcttcgcgag tggtttcgat ttcgaaaccc tcgtcgattg cgtggacttc
     1141 aagatccggc aataaatctg atttttcgtt ctcgttactc acgttgaaat tattgtacgt
     1201 cgatcaggcc aggggctgca aatttcttga agataagaaa tccgtcggta tcgatcactt
     1261 tctgataggc tgcggggtcg agcgaggcgg caactttttc caggttgttc accccccatt
     1321 gagagcgcca cgggcttaga tcgaacacga cataatcggt cgtgggcggg gtgccacgga
     1381 aaaggtccgc ctgggtccga gtgcaaacgt gcgggtgcac gatcatttga gccgtaaccg
     1441 aaacgttagg tggaatttgt tcgagcacct ttgaaataaa ggcatcgcgc tcatcagggc
     1501 gaaactcgcg cacggtaaac gccggactac gcccgaaaaa cagcataaac aaaagcacga
     1561 gcgtcatgag cagctcaggc cgaccggggt tcaagcgtcg cgaaaaaaaa gaaacaccgc
     1621 cgctgagcaa agtttgcacg ccacttatca atacgtagaa taaaaacgga atgagcacgc
     1681 actcatagtg aaaaccgaat ttaaggtcga gagccgtatt catggcaaga ccagcgcagg
     1741 caattaaaaa gggaatcggc gcggcccaag caaacggcac aaatggcgtg agcactttta
     1801 acacgtaacg cagattctcg cgctgaatca tgcgagccca aacttcttct aaaagttgta
     1861 gggggcgaac agtcgaggct tccacgattc cggcggcgtc taatccgaaa cgcttggcca
     1921 gcatggtggg cccggcacct gaaaaatagg gcgttacgta aaggtttact acactcaaca
     1981 caatcaacga agtcactgcg aggctcacgc ccaccaaaac gtgacgttgt ttttttcgcc
     2041 ccgccaccca gagaccaagt aacatcgtaa aaattccggc gttttctttt atgaaccatg
     2101 cagcaacaca agaaagcaca aagagcgtca tgcgctgccg ttccaacgct aatagcgcac
     2161 ccaagaaaac tggaacgaat aaattgtctt cgcgtaaatc gaaaatatta gccattcgaa
     2221 gtggctggta gattaaaaac agagaaccca gaaccacggc ccattcccgg ggcacttttt
     2281 ttgcgcgtgc aagtttgtac accaacaccg cagaaagccc cagcaacgag gcctgcgcca
     2341 ggaacagcga atacgcttcg ctccacaaac ggtaaatcgg cgtgaaaagc gcgagcaagg
     2401 gtgaaa
//
LOCUS       DAOMXO010000363         2405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25434_length_2405_cov_4.360851, whole genome shotgun sequence.
ACCESSION   DAOMXO010000363 DAOMXO010000000
VERSION     DAOMXO010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25434_length_2405_cov_4.360851"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLH57_07890"
     CDS             <1..250
                     /locus_tag="PLH57_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02573.1"
                     /translation="KHDIGSPGRDPGFKFRKQQIQNFYYLLRKNVGLTPKSISAFSWY
                     CFGQLLDDSRNLRRSALKGCLAGIINVARGRLDSVRSD"
     gene            complement(247..864)
                     /locus_tag="PLH57_07895"
     CDS             complement(247..864)
                     /locus_tag="PLH57_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRK02574.1"
                     /translation="MSYVQKYLEEVTEIARRVDRGQVEKMIEVLTQLQRTGGRLFFLG
                     VGGSAANCSHAVNDFRKLAGIETYSPVDNVAELTARTNDEGWDTVFAEWLKVSRLSSK
                     DAVFVFSVGGGNREKNVSVNLVKALELAKETGTKILGVVGRDGGFTAKVADACVIIPT
                     LSTDTITPHAEAWQGVIWHMLVSDPRVLKKQNKWESLSPKESATV"
     gene            905..2329
                     /locus_tag="PLH57_07900"
     CDS             905..2329
                     /locus_tag="PLH57_07900"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRK02575.1"
                     /translation="MVNRIPPRLRPYYAFLITLAQTVADLSAVAVSFWFGYYVYIGSG
                     APAPQTPSEYWLIVAMAVGCYFLIFRILNLYEREISLLNVEELKKIFMATIWASFLFF
                     GLAFYIRSVSLSRIMITVSIVTAGLLVYLERILFYRLHIWFHENGFSRENVLVYGAGY
                     VGKHLVKRLSSSPGLGYLPIGFMDDDSQRFGQIVTFKDGGPRHGLLVFGGLDDLERVV
                     KENQVREVFIAMPNATYERNLEIINKCRALGVSFSIVPNAYQQFVQKISATEIGGIPL
                     LRNKSRKDTLGYLVAKRVLDFFISSLLIVALSPLLMVFSILIKIDSRGPVIFKQKRVG
                     LRGKEFSFYKFRTMRVDAPKYALTPQSTDDPRITRVGRWLRRTSLDELPQLFNVFRGD
                     MSLVGPRPEMPFIVEKYDELQRERLSVKPGITGVWQISCVRGEPIHENVEYDLFYIEH
                     RSMLLDIAILLKTIAGVVRGIGAV"
BASE COUNT          588 a          610 c          549 g          658 t
ORIGIN      
        1 caaacatgat ataggttcgc cggggcgtga ccccggtttc aagtttcgaa aacagcaaat
       61 tcagaatttt tactacctat tacgcaaaaa tgtcggactc acacccaaat ccattagcgc
      121 cttctcatgg tactgttttg gtcaattgct ggacgattct cgaaatttac ggcgaagcgc
      181 cctcaagggc tgcttagccg gaataataaa cgtcgctcga ggacgcctcg actcggtacg
      241 ctcagattaa accgtcgccg attcttttgg cgaaagagac tcccacttat tctgtttttt
      301 aagcacccgg gggtctgaga ccaacatatg ccaaatcaca ccctgccagg cttcggcgtg
      361 tggagtaatc gtgtcagtac tcagggtcgg aataatcacg caagcatccg caactttagc
      421 ggtaaagccc ccgtcgcggc caacgacgcc taaaattttc gtgcctgttt ctttggccaa
      481 ctcaagtgct tttaccaaat tcacgcttac attcttttcg cgatttccgc ccccaacact
      541 gaatacaaaa actgcgtctt tcgaactcaa acgactaacc ttcagccatt cggcgaaaac
      601 ggtatcccaa ccttcgtcgt tggtgcgcgc cgtgagttca gcgacgttat ccaccggtga
      661 ataagtttcg atacctgcca gcttccgaaa atcgttcaca gcatgcgaac aattcgctgc
      721 acttccgcca acccccaaaa aaaacaaccg gccgccagtg cgctgcaact gggtcaaaac
      781 ttcgatcatt ttttcgacct gcccgcggtc aacgcgacgg gctatttccg taacttcctc
      841 aagatatttt tgaacgtagc tcattgattg aaattgcgtg ttttaaggca taacgtcaag
      901 ggcattggtt aaccgaattc caccgcgatt gcggccctat tacgcctttt tgattacact
      961 tgcccagacg gtcgccgatc tatcggccgt cgccgtttcg ttttggtttg gttattacgt
     1021 ctacattggc tcaggtgccc ccgccccaca gactccgagt gagtattggc tgatcgtcgc
     1081 tatggcggtg ggttgttatt tcttgatctt tcgcattctg aatctttatg agcgcgaaat
     1141 tagcctttta aacgtagaag agttgaaaaa gatcttcatg gctacgatct gggcaagctt
     1201 cttgtttttc ggactagcct tttacattcg atccgtatcg ctctcccgga tcatgatcac
     1261 cgtgagtata gttacggcgg gccttttggt ctatctcgag cgcattctat tttaccgcct
     1321 tcatatttgg ttccatgaaa acggcttttc tcgggaaaac gtgttggttt acggcgcggg
     1381 gtacgtcggc aaacatttgg tgaaacgcct gtcttcgtct cctggactcg gttatttacc
     1441 gattgggttc atggacgacg actcacaacg atttggccaa attgttacct tcaaagatgg
     1501 cggaccccgc cacgggctgc tcgtttttgg tgggttagat gacctcgaac gagtggtgaa
     1561 ggaaaatcaa gttcgagagg tgttcatcgc tatgccgaat gcgacctacg agagaaacct
     1621 cgagatcatt aataaatgtc gcgcgctcgg agtttcgttc agcatcgtac ccaacgccta
     1681 tcagcaattt gttcaaaaaa tctcggctac tgaaataggt ggcattcccc tgctgcgcaa
     1741 caaatcaaga aaagacactc tcgggtatct agtggccaaa cgagtgctcg acttttttat
     1801 ttcttctctt ttaattgtag cacttagccc tttgttgatg gtgttttcga tcctcataaa
     1861 aatagattct cgcgggcccg tcatctttaa acaaaaaaga gtagggttaa gaggcaaaga
     1921 gtttagtttt tacaagtttc gcaccatgcg agtagacgct cctaaatacg cactcacacc
     1981 gcaatcgacc gacgacccca gaattacgcg agtgggccgc tggttgcgac gtacaagtct
     2041 tgacgagcta ccgcaactat ttaacgtttt tcgaggagac atgagtttag taggaccccg
     2101 ccccgagatg ccgtttatcg ttgaaaaata tgatgaactg caacgagagc gattgtcagt
     2161 taagccgggc attactggcg tttggcaaat ttcatgtgtt cgcggagagc ccattcatga
     2221 aaacgtcgaa tatgatttgt tctacatcga gcatcgatcc atgttgcttg atattgcgat
     2281 cctattaaaa acgatcgcag gtgtggtgcg tggtatcggc gctgtataaa ttggtccctc
     2341 gtaacaccag atccacagca agccccatgg cccgaatttc gtccagataa tcacgatgaa
     2401 atgct
//
LOCUS       DAOMXO010000364         2403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25466_length_2403_cov_5.648637, whole genome shotgun sequence.
ACCESSION   DAOMXO010000364 DAOMXO010000000
VERSION     DAOMXO010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25466_length_2403_cov_5.648637"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1431
                     /gene="lpdA"
                     /locus_tag="PLH57_07905"
     CDS             28..1431
                     /gene="lpdA"
                     /locus_tag="PLH57_07905"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008383378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRK02576.1"
                     /translation="MEQYDVVYIGGGPAGYVGAIRSAQLGMKTACVEKRKALGGTCLN
                     IGCIPSKALLDSSEHFAFVKHKAAKHGLVVGDLKIDLGQMMKRKDQVVTGLTTGIAGL
                     FKKNKIAHLVGHGRITAPGTVEVVAENGAKETISAKNIVIATGSDVARIPVAALDGVR
                     VISSTEALALPQVPKSMVVIGGGAIGLEMGSVWMRLGADVTVVEYNERIASFADHQIS
                     GELLKILQRQGMKFKLATKVVNVQNVGSKAAVEMEPAAGGTRETIEADVVLVAVGRKP
                     FTDNLGLDKIGLATDKAGRIEIDNHFRTKVAGVYAIGDVVRGPMLAHKAEDEGIAVAE
                     IMAGKAGHVNYDAIPSVIYTWPELATVGMSEQDCKAKEIAVRTGTFPFIANGRAKAMD
                     ESEGMIKIIADAKTDRLLGLHMVGPRASDMIAEAVMVMEFGGSAEDIALSTHAHPTLP
                     EAMKEAALAVHKRAIHF"
     gene            1461..1943
                     /locus_tag="PLH57_07910"
     CDS             1461..1943
                     /locus_tag="PLH57_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRK02577.1"
                     /translation="MRVVSFHYVLKNKSGELLDSSSGGEPMTYLEGSGQIIPGLESQL
                     KSCASGDKKKVEVKAKDAYGEKDSELAFDVPRTQFPPNEDIQVGMQFKIGGPGSDQEA
                     SPVFTVVKTSATHVSVDGNHPLAGQDLFFDVEIVEIRAATPEELSHGHAHGRGGHHHH
                     "
     gene            1946..>2403
                     /locus_tag="PLH57_07915"
     CDS             1946..>2403
                     /locus_tag="PLH57_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRK02578.1"
                     /translation="MRIDDRVAVVGSGPAGLMVADLLSRSGASVVLFEKNPALARKLL
                     IAGSSGLNVTHSTSEETFHERYMGAPNAKFWSEILRAFSPKDWLQFLNELGQQTYLGT
                     SGRYFLKNMKASKLVKAWIDRLEQQGVRIESDTKVTDFDSTSSGPQLRYQT"
BASE COUNT          632 a          542 c          674 g          555 t
ORIGIN      
        1 caacttcttt aggatttgag ccgtaaaatg gaacagtacg atgtagttta tatcggtggt
       61 ggcccggccg gatacgtggg tgcaattcgc agtgcccagc ttggcatgaa gaccgcttgc
      121 gtcgaaaaac gaaaagcact gggcgggact tgtctcaaca ttggctgtat tccttcgaag
      181 gccctgcttg attcgagtga acactttgca tttgtgaaac acaaagccgc caaacacggt
      241 ctggtggtcg gagatcttaa aattgatctt ggccaaatga tgaaacgcaa agatcaagtt
      301 gtaacgggac ttacgactgg aattgctgga ctgtttaaaa aaaataaaat tgctcatttg
      361 gtcggtcacg gccgcatcac ggcgccgggc acggtagaag tggttgctga aaatggtgcg
      421 aaagaaacga tttctgcgaa aaacatcgtg attgccactg gtagtgatgt ggccagaatt
      481 ccggttgctg cgctcgatgg cgtacgagtg ataagttcga ctgaagcgct ggcgctgcct
      541 caggtgccaa aatccatggt cgtcattgga gggggagcga ttggacttga aatgggttcg
      601 gtctggatgc gtttgggcgc tgatgtcaca gtggtggaat acaacgaacg catcgcgagt
      661 ttcgccgatc accaaattag cggtgagttg ttgaaaatat tgcaacgcca aggaatgaaa
      721 ttcaaactcg cgaccaaagt ggtgaacgtt caaaacgtag ggtcgaaagc agctgttgag
      781 atggagccag cggcgggcgg tactcgtgaa acgatcgagg ccgacgtagt attggtagca
      841 gtgggacgta agcccttcac cgacaattta ggactcgata aaattgggct tgctaccgat
      901 aaggctggcc gcattgaaat cgacaatcat tttcgaacaa aggtagcagg cgtgtacgca
      961 atcggcgacg tagtgcgggg gcctatgctt gcccacaagg cggaagacga aggcatcgca
     1021 gtagctgaaa taatggctgg taaggctggc cacgtgaact acgacgctat tccgagtgta
     1081 atctacactt ggccggaact cgcaacagtt ggtatgagcg aacaagactg caaggccaaa
     1141 gagatcgcgg ttcgcacagg gacctttccc tttattgcca acgggcgtgc taaggccatg
     1201 gacgaatccg aaggaatgat caaaataatc gctgacgcaa aaacggaccg acttttgggc
     1261 ctgcatatgg taggtcctcg ggcgtctgac atgattgcag aggccgtcat ggtgatggag
     1321 tttggtgggt cagcggaaga cattgcgctc tcaacgcacg cccaccccac gcttcctgag
     1381 gccatgaaag aagcggcgct cgctgtacac aaaagagcga ttcattttta acggctctaa
     1441 tcaaagaaaa gaggcattct atgcgtgttg taagttttca ctacgttctc aaaaataaaa
     1501 gcggtgagct actcgatagt tcttcaggcg gcgaacccat gacttacctt gaaggtagcg
     1561 gtcaaatcat tccaggactg gaatctcagt taaaaagctg cgctagcgga gataagaaaa
     1621 aagtagaagt gaaagctaaa gacgcttacg gcgaaaaaga tagcgaacta gcattcgacg
     1681 tgccacgcac tcagttccct ccaaacgaag acattcaagt aggaatgcaa tttaaaatcg
     1741 gtggtccggg ttcggatcag gaagcgtctc cggtatttac cgttgttaag acttcggcaa
     1801 ctcacgtgag cgttgatggc aaccacccac ttgcgggaca agatttgttt ttcgacgttg
     1861 aaatcgttga aatacgcgct gctactccag aagaattatc tcatggtcac gcccacgggc
     1921 gcggcgggca ccaccaccac tgagcgtgcg gatcgacgac cgagtcgctg tagttggatc
     1981 aggtccagcc ggtttaatgg tggccgatct gctttcgcga tcaggcgctt ctgtcgttct
     2041 ttttgagaaa aatcctgctc tggcgcgaaa gttgttaatc gccggaagtt cgggcctcaa
     2101 tgtgacgcac tcgacctccg aggagacgtt ccacgagcgt tatatgggcg cgccaaatgc
     2161 caagttttgg tctgaaattc tccgggcgtt ctcgcccaag gattggctgc agtttcttaa
     2221 tgagttgggc cagcagactt atcttggaac ctctggtcga tattttttga aaaacatgaa
     2281 agcttccaag ttggtgaagg cttggattga ccgccttgaa caacagggcg tgagaattga
     2341 gtccgacacc aaggtgacgg acttcgattc tacttcgagt ggtccacaat tgcggtatca
     2401 gac
//
LOCUS       DAOMXO010000365         2401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25497_length_2401_cov_4.912617, whole genome shotgun sequence.
ACCESSION   DAOMXO010000365 DAOMXO010000000
VERSION     DAOMXO010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25497_length_2401_cov_4.912617"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLH57_07920"
     CDS             complement(<1..750)
                     /locus_tag="PLH57_07920"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRK02579.1"
                     /translation="MRRAKLLLRTVVLTSFFCACSAQQIVKREYTETGLPEDLVKAFA
                     VKEIAAPLDASKLGPTESGAQAEKKPKKKKRKTKGLMGDTKSTFVIPNRRPPKDPVWV
                     GEKIFMDVTWLNTTAGEFLLEVNPYKELNGRKVYDIKGSAKTSSLFSLIYKAEDWVQT
                     FIDFEGWFPYKFILHGDETRYVRSNLELFDHAAKKQFVSVYAKRLDKDQIKDEKGYKD
                     LVPFSQDSLSALYFARTHKMDPGTEIRFPMST"
     gene            complement(762..1655)
                     /locus_tag="PLH57_07925"
     CDS             complement(762..1655)
                     /locus_tag="PLH57_07925"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02580.1"
                     /translation="MSPLLSLLIQAVLSPLGLLATFMPRGLELMVGPILGRGLFLVSR
                     RRSVARENLLRCFPEWGPSKIDHVLKKNYEHYGILVFELLHLFSPIPGHYLRYVKRNT
                     HVMGLENWRAAHAKGKGVLFVASHLANWELMVSGGALAGIPITMVTKHLKPEWLHRKI
                     EKSRNSTGVRAAYEPRTLPIIMRALRAGESVGFVLDQYAGPPIGIKVPFFGVSVGTLA
                     AVGTLSNRTGALVVTAKTFRDTDGRINICMNPALDLGELAQDEVRTTALLAKMVENWI
                     REFPDQWLWIHRRFKNIDPMV"
     gene            complement(1615..>2401)
                     /locus_tag="PLH57_07930"
     CDS             complement(1615..>2401)
                     /locus_tag="PLH57_07930"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRK02581.1"
                     /translation="VSTRGYGSSWEQRSGYATDFESAHTQKFPDEALVLLKKTPGVAV
                     AVGKRRVDTLKRNWENLRPDVIILDDGFQHFSMNRQIDVLVHNFEIRNPLFRDFPFWF
                     NRAGLRVAFSDVPAKLNNGPPWVRVNYRLKGVQKVNSKLESIPRTANVFCGIGNPNRI
                     RSFLERNGVKVNAMKVFRDHQDYHLADIEGLKKWNEKNGEFPLVTTLKDAVKLRDYAQ
                     CMGGVSGFDPLWLDIGVEILDHKEYFWRKLNESVALASDSGGP"
BASE COUNT          609 a          617 c          534 g          641 t
ORIGIN      
        1 ggtgctcatt ggaaagcgaa tttcagtgcc aggatccatt ttgtgagtac gcgcaaagta
       61 gagtgcactc agagaatctt gcgagaatgg cactagatct ttgtagcctt tttcatcttt
      121 aatctggtct ttatctaaac gtttcgcata aaccgatacg aattgtttct ttgcggcatg
      181 atcaaaaagc tccaggttgc ttcgcacata acgagtctcg tcgccatgaa gaatgaactt
      241 atagggaaac caaccttcaa aatctatgaa agtttgtacc caatcttccg ctttgtagat
      301 tagggaaaat aaactggagg ttttagcaga tcccttaatg tcgtaaacct tacgcccgtt
      361 aagctctttg tagggattta cttctaaaag aaattcaccg gccgtggtgt taagccaggt
      421 gacatccata aatatcttct cacccaccca caccgggtct ttgggcggtc gtcggttggg
      481 tattacaaat gtgcttttgg tatcgcccat gagccctttg gttttccttt ttttcttctt
      541 aggttttttt tccgcttggg cgccggactc ggtcggccca agtttagaag catctaaagg
      601 ggcggcaatt tcttttaccg cgaacgcctt cacaagatct tctggcaatc cggtttctgt
      661 atactcgcgc ttaacaattt gctgtgccga acatgcacaa aaaaaactgg ttaggactac
      721 cgttcggaga agaagtttcg cgcgtcgcat gggcctctta tttataccat ggggtcaata
      781 tttttaaaac gcctgtgaat ccacaaccat tgatccggaa actctcgtat ccaattttcc
      841 accattttcg ctaacaaggc ggtggtgcga acttcgtctt gcgcaagttc ccccaaatct
      901 agcgcggggt tcatgcaaat gttgattcga ccgtcagtgt ctcgaaaggt tttggcggtc
      961 actactaacg cgccggtgcg attcgacaag gtcccaaccg ctgccagtgt tccgaccgat
     1021 acgccaaaaa agggtacctt tattccgatt ggaggccccg catattgatc caatacgaag
     1081 ccaaccgact ccccagcacg caaagcacgc ataattatcg gcagagtacg gggttcgtag
     1141 gccgcccgca ctccagttga gttccgcgat ttttctattt ttcgatgcaa ccactctggc
     1201 ttgagatgtt tggtcaccat tgtgataggg atcccagcta gagccccccc tgaaaccatt
     1261 agttcccaat tcgctaggtg cgacgctacg aacaatacgc cctttccttt agcgtgtgcc
     1321 gcgcgccaat tttctaaccc cataacgtgg gtatttcgtt ttacgtatcg cagatagtgc
     1381 ccaggaatgg gcgaaaaaag atgcaataac tcaaaaacca atattccgta gtgctcgtaa
     1441 ttctttttga gaacatgatc aattttgctt gggccccact cgggaaaaca gcgcaaaaga
     1501 ttttcgcgcg cgacacttcg gcggcgcgag accaaaaaca gaccgcggcc caaaatcgga
     1561 cctaccatga gctcaagccc acgcggcatg aatgtcgcga gcaatccaag gggactaagg
     1621 accgcctgaa tcagaagcga gagcaacgga ctcattaagt tttctccaaa agtattcttt
     1681 atgatctagg atctcgactc caatatcgag ccacagtggg tcaaaacccg aaacgccccc
     1741 catgcactgg gcgtaatcgc gcaacttaac ggcgtccttt aaggtggtaa caagaggaaa
     1801 ttctccattc ttctcattcc actttttgag tccttcgata tcggccaagt ggtagtcctg
     1861 atggtcgcga aataccttca tggcattaac cttaaccccg ttgcgttcaa gaaacgatcg
     1921 aatcctatta gggtttccta tgccgcaaaa tacgttggct gtccgtggta tagactccag
     1981 tttgctgtta actttttgta cccctttaag tcggtagttt acacgaaccc acggaggccc
     2041 attattcaac ttagctggga catccgaaaa cgccacccgc aatccagctc gattgaacca
     2101 aaaaggaaaa tcccgaaata gcggatttcg aatttcgaag ttatgcacca agacatcgat
     2161 ttgacggttc atggaaaagt gctgaaaccc gtcatctaaa ataatcacat cgggacgtag
     2221 attttcccag tttcgtttca gcgtgtcgac tcttcgcttt cccactgcga ccgcgactcc
     2281 gggtgttttt ttaagcaaaa ccagcgcttc gtcgggaaat ttttgtgtgt gggccgactc
     2341 gaaatctgtc gcgtaccccg atcgctgttc ccacgaagaa ccgtaccctc gcgttgaaac
     2401 g
//
LOCUS       DAOMXO010000366         2393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25623_length_2393_cov_5.147562, whole genome shotgun sequence.
ACCESSION   DAOMXO010000366 DAOMXO010000000
VERSION     DAOMXO010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25623_length_2393_cov_5.147562"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLH57_07935"
     CDS             <1..225
                     /locus_tag="PLH57_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3463 domain-containing protein"
                     /protein_id="HRK02582.1"
                     /translation="VFGWQRPCYLFSENGYAATFAELMNTTDWDKYGSRSGHPKCRDC
                     MVHCGYEPAAVNDSMAGPRNIIRSITAAMR"
     gene            228..1262
                     /gene="fni"
                     /locus_tag="PLH57_07940"
     CDS             228..1262
                     /gene="fni"
                     /locus_tag="PLH57_07940"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRK02583.1"
                     /translation="MSVLEGETDRKNSHLRICLDESVDYYDERLNGFARYRLEHDALP
                     EIRKADIDLSVELFGKKLKAPLLIGAMTGGTERAAEINRRLAIAAERCGVGFALGSQR
                     KMLEDPSLIASYSVKKWAPNLPLLFGNVGAVQLNYGVTPKDIRRLVEAVECNVLNLHL
                     NPLQEAIQPEGDTDFRGLVDKIRETISQVGVPVVLKEVGAGISNATLQKIRTLPVAGV
                     ETAGVGGTSWSKIESLRTSNSIQKSTGELFATWGVSTAESVIACRNALPDKAVIASGG
                     IRTGIEIAKALVLGADAAAMALPMLKAAEQSIEQAVNAIERVIEELRVTMFVTGSSTI
                     SELKKKRTTT"
     gene            1259..2194
                     /gene="mvk"
                     /locus_tag="PLH57_07945"
     CDS             1259..2194
                     /gene="mvk"
                     /locus_tag="PLH57_07945"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HRK02584.1"
                     /translation="MKVVGRSHGKFILLGEHFVVHGGVAALAFPMKDLTCEVTIVPDT
                     VPMYRAEVPNTPERDIVENVMARAVYIAADCFRVDIKGQPIRVESKTNFPVSRGFGSS
                     AAFAVALARAFDEYRTEVAKVRADWMEIERAVSAIETLFHGNPSGVDAATILAGRPIR
                     YENRAVVREVQNKALDFVAIDSGLRENSAPLIARITEFKHKNLDRWTEMTNELKTAID
                     NAEQGLNYGDAAVVVESIRKSQGILSELGLSNETIDALIYEACANGALAGKVSGAGGG
                     GAVILAAPRTKGRELAARMTERGYSVLGIEHGESA"
     gene            2191..>2393
                     /locus_tag="PLH57_07950"
     CDS             2191..>2393
                     /locus_tag="PLH57_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02585.1"
                     /translation="MIRSVELLPKDWELVPVVEKNAVRVFSPSNIAVAKYWGKRDLAL
                     NLPTNDSVSITLKDFGSFTTVEF"
BASE COUNT          657 a          483 c          668 g          585 t
ORIGIN      
        1 gtctttggtt ggcaacgccc gtgttatcta ttcagtgaaa acgggtatgc agctactttc
       61 gcggaactta tgaacacaac agactgggac aagtatggtt cgcgtagtgg tcacccaaaa
      121 tgccgagatt gcatggttca ttgtggttat gagccggcgg cagtgaatga cagcatggcg
      181 ggaccgcgca atattattag gtcgattacc gctgcaatga ggtaaaattg tcggttttag
      241 aaggcgaaac agacagaaaa aatagtcatc ttaggatctg tctggacgaa agcgtggatt
      301 attacgacga gcgcctcaat gggtttgctc ggtatcgcct agaacatgac gcgttacctg
      361 aaattcgcaa ggcggacatc gatctttcgg tcgaactttt tggtaaaaaa ttgaaagctc
      421 cgcttttaat aggcgcgatg actggtggta cagagcgggc tgcggaaata aaccgacgat
      481 tggcaattgc ggccgagcgt tgtggagttg gtttcgcgtt gggctctcaa cgtaaaatgt
      541 tggaagaccc gtccttaatt gcttcgtatt cggtaaaaaa atgggcgcca aatcttccgt
      601 tactttttgg aaatgttgga gcggttcaat tgaattacgg agtgacaccg aaagatatac
      661 gtcgtttggt cgaagcggtt gaatgtaatg tgttaaatct ccatttgaat ccgctacagg
      721 aggccataca acccgaaggg gacactgact ttcgagggct ggtcgataaa atccgtgaaa
      781 ccatttcgca ggtcggagtg cccgtagtcc taaaagaggt tggagcagga atttccaacg
      841 ctacattgca gaaaattcga acgctaccgg tcgcgggagt ggagacggcc ggagtcggtg
      901 ggacctcctg gtcgaaaatc gaaagtctac gaacctccaa ttcaattcag aaatctactg
      961 gtgagttgtt cgccacctgg ggagtatcta ctgcagagag cgtaatcgcc tgtcgaaatg
     1021 cattgcccga taaagcagta attgcttcgg gagggattcg gaccggaatt gaaattgcaa
     1081 aggcactggt gttgggggcc gatgctgcag caatggcgtt gccaatgttg aaagcagctg
     1141 aacaatcaat tgaacaagcg gtaaacgcga tcgaacgagt aattgaagaa ctacgggtaa
     1201 ccatgtttgt cacaggttct tcgacaatct cagaactcaa aaagaagagg acaacgacat
     1261 gaaggttgtg gggcgatctc atggaaagtt tattttgctt ggtgaacatt tcgtagttca
     1321 cggcggagtt gccgccttgg cgtttccgat gaaagatctg acatgtgaag ttaccattgt
     1381 gccagacaca gtgccaatgt accgagccga agttcccaac actccagagc gagacattgt
     1441 ggaaaatgtt atggcacggg cagtgtatat tgccgccgac tgttttcgag tcgacattaa
     1501 aggccagcca attcgggtag agtcgaaaac caatttcccg gtatcgcgag gcttcggtag
     1561 tagtgcagcg ttcgcggtag cattggcgag ggcgttcgac gaataccgta ccgaagttgc
     1621 aaaagtacga gccgattgga tggagattga gagagctgtt tctgcaattg aaactttatt
     1681 tcatggcaat ccgagtggcg tcgatgccgc aacaatcttg gcggggcgac caatacgtta
     1741 tgagaatcgt gcggtggttc gcgaagttca aaataaagcg cttgatttcg tagctattga
     1801 tagtgggctt cgggaaaata gtgccccact aattgcgcgc ataactgagt tcaaacacaa
     1861 gaaccttgat cgatggacag aaatgaccaa cgaattaaaa accgcaattg ataacgctga
     1921 acaagggtta aattatggtg atgctgcggt ggttgtagag tcgattagaa aatcccaagg
     1981 gatattgtcg gaacttggcc tgagcaatga aacaattgat gcattaatat atgaggcgtg
     2041 cgcaaatggt gcgctagcag gaaaagtaag tggagctggg ggcggcggag cagtgattct
     2101 tgcagctcct cgaaccaaag ggcgagaact agccgcgcgc atgaccgaac gaggatattc
     2161 agtgctgggc atagaacacg gagagtctgc ttgattcgaa gcgtagagct tttaccaaaa
     2221 gattgggaac tcgttccggt tgtagaaaaa aacgcggttc gagtattttc tcccagcaac
     2281 attgcggtcg ccaagtactg gggcaagcgc gatcttgccc tgaatcttcc tactaacgac
     2341 agtgtttcga tcacgttaaa agattttgga agttttacta cagttgagtt ttt
//
LOCUS       DAOMXO010000367         2382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25788_length_2382_cov_6.181349, whole genome shotgun sequence.
ACCESSION   DAOMXO010000367 DAOMXO010000000
VERSION     DAOMXO010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25788_length_2382_cov_6.181349"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1033
                     /gene="fliP"
                     /locus_tag="PLH57_07955"
     CDS             182..1033
                     /gene="fliP"
                     /locus_tag="PLH57_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941092.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRK02586.1"
                     /translation="MSTVKILLTLSLLCLMFSNATNAQTARLNTSGQPVAQGPPKTSS
                     QDANVVQRSGNAGTLGIPTLSFGLTEAGKPTEVANALKVLVMLTVLSLAPAILILMTS
                     FTRIVVVLSFLRQAIGTQQMPPNQLIVGLSLFLTFFVMAPTFKNIQTTAIEPYMKDQL
                     TQWDALSKAESPMRAFMFQQTREKDIEAMMGMAKLGRPQNRDAVPTYVLIPSFILSEL
                     KTAFQMGFLLYLPFLVIDMVVSSVLMAMGMMMLPPVVISLPFKLLLFVLVDGWQLVVG
                     SLVKSFG"
     gene            1045..1320
                     /gene="fliQ"
                     /locus_tag="PLH57_07960"
     CDS             1045..1320
                     /gene="fliQ"
                     /locus_tag="PLH57_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRK02587.1"
                     /translation="MSEDLILGIGHEALKVAIFLAGPILLVAMIVGIAISMLQAVTQI
                     NEATLSFVPKILAISIVLVVTAPWMIETITTYTTELIQQIPDYIANN"
     gene            1341..2144
                     /gene="fliR"
                     /locus_tag="PLH57_07965"
     CDS             1341..2144
                     /gene="fliR"
                     /locus_tag="PLH57_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRK02588.1"
                     /translation="MNLFPINEVQLMTAFFVLVRVSAIIALLPLFGDRTVPPTIKVLF
                     SVAMSFVVFPTLWQSGLRFSDVKSQSTGLLVGAVVSELFVGLLLGFFARWIFDAVRAA
                     GYFMGTQMGFSMGSVLDPTTETQTIALAEFQYGLAALLFLAMDGHHVYVNSIMDSFRL
                     IPLGELPVLSKGDTMIRTLIDLTEKVLLLSVHLAAPVLIVMLLVNLAFGLVSRAVPQI
                     NMLVISFAANILVGLMIFAVSLPGFHALVGTQFEEYSQQLRSFMTLLGN"
     gene            2145..>2382
                     /locus_tag="PLH57_07970"
     CDS             2145..>2382
                     /locus_tag="PLH57_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HRK02589.1"
                     /translation="MAEYEERSLDDLTEEPSPQRLEELRKKGTVPQSRDLTSTLVLLG
                     VSCALYLFSNMAVSELSTLMREVFSKDLASKLTLE"
BASE COUNT          555 a          521 c          593 g          713 t
ORIGIN      
        1 actgcacgct atgacggtcc aaaagacttt ggtgctggcc cgcaagatgc attaatgagt
       61 gcaacagaac gcacacgtga ccgtgttgaa atttcacgcg ctgaatctac cagcacggtc
      121 cgcaccggta ttcgagaaaa gttaaccaat ttaaagccgt tgaattagga gcgatgatta
      181 gatgagcacg gtgaaaatac tattaacgct ttcactgctt tgtctgatgt tttccaatgc
      241 taccaacgcg caaaccgctc gcttaaatac ctcgggtcaa ccagtcgcgc agggcccgcc
      301 aaagacttca tcccaagacg cgaacgtggt gcaacgttcg ggcaatgctg gcacgttggg
      361 tatccccact ctttcgtttg gtctgaccga ggctggtaaa cctaccgaag tagcgaatgc
      421 attaaaagtt ttagtgatgc tcacggtgct ttcgcttgcg cccgcgatat tgatcttgat
      481 gacttcattt acgcgcatcg tggtggttct tagtttttta cgacaagcaa ttggtactca
      541 gcagatgccg cccaaccaat taattgtggg actatcgttg tttctcacct tctttgtaat
      601 ggcgccaact tttaaaaata ttcagaccac ggcgatcgag ccctatatga aagatcagct
      661 aacgcaatgg gacgcactgt cgaaagcaga gtctccaatg cgtgcattca tgtttcagca
      721 aacacgggaa aaagacattg aagcaatgat gggaatggcg aagttaggaa gacctcagaa
      781 ccgtgacgcc gttcctacgt acgtcttaat tccgtcgttt attttgagcg aacttaagac
      841 cgcttttcag atggggtttt tgctgtatct accgtttctc gtgatcgaca tggtggtttc
      901 cagtgttttg atggccatgg gcatgatgat gttaccgccg gttgtgattt ccttgccctt
      961 taagttgtta ctgtttgttt tggtcgatgg ctggcaactc gtagttggtt cactagtgaa
     1021 gtcatttggt tgagagagga tacaatgagc gaagatctca ttttaggtat aggacacgaa
     1081 gccttaaagg tggccatatt tctagcaggc ccgatactct tggtggccat gatagtaggt
     1141 atcgcgattt caatgctgca agcagttaca caaattaacg aagcgacgtt gtcatttgtc
     1201 ccaaagattt tggcaattag cattgttttg gtcgtaactg ctccatggat gattgaaacg
     1261 attacgactt acaccacgga acttattcaa cagattccag attacattgc caataactag
     1321 acgttgagta gtgggttagc atgaacttgt ttccaataaa tgaagtgcaa ttgatgaccg
     1381 cattttttgt tttggtgcgc gtgtcagcta ttattgcgct cctcccactt tttggtgatc
     1441 gcaccgtacc accgacgatt aaagttctgt ttagcgtcgc tatgagcttt gtggtttttc
     1501 ccactctatg gcagagcggt cttcgttttt cagacgtgaa atcgcaatct accgggctcc
     1561 tggttggcgc ggtagtgtca gagttgtttg tcggattgct gttgggtttc tttgcgcgtt
     1621 ggatattcga tgcggtacga gcggctggtt attttatggg aacgcagatg ggattttcta
     1681 tgggttcagt gctcgatcct accactgaga ctcaaaccat tgcgttggct gagtttcagt
     1741 atggtttggc tgcacttttg tttctggcaa tggatggcca tcacgtttac gtcaattcta
     1801 ttatggattc ttttcggttg attccgcttg gtgaattacc agtgctgtcg aaaggggata
     1861 cgatgattcg tactctaatc gatcttaccg aaaaagtgct tctcctttcg gtgcaccttg
     1921 ctgcgccggt cttgattgta atgttgttag tcaatttggc atttggcctg gtgtcgcggg
     1981 ccgtaccgca gatcaacatg ctcgtaataa gttttgctgc caatattttg gtaggcttaa
     2041 tgatattcgc ggttagtttg cccggctttc atgcgttagt gggcactcag ttcgaggaat
     2101 attcgcagca gttgcgatct ttcatgacgc tgttggggaa ttagatggca gaatacgaag
     2161 agcggtctct cgatgactta acagaagaac cctcaccgca gcgactcgaa gagttgcgaa
     2221 aaaaaggcac cgtacctcaa agccgtgacc taacgagtac tctggtgctg ttgggcgttt
     2281 cttgcgcgct ctatttattt tcaaatatgg ccgtttctga gctgtcgacc ctcatgcgtg
     2341 aggtattttc gaaagacctc gcctcgaaac taacgctcga ag
//
LOCUS       DAOMXO010000368         2381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25809_length_2381_cov_5.089854, whole genome shotgun sequence.
ACCESSION   DAOMXO010000368 DAOMXO010000000
VERSION     DAOMXO010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25809_length_2381_cov_5.089854"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..504)
                     /locus_tag="PLH57_07975"
     CDS             complement(223..504)
                     /locus_tag="PLH57_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02590.1"
                     /translation="MKKNSRSLSPKLKIFLVIAAGAAFMGSFAWIRLEIVRISYDIHR
                     LEKLEQQARDDVNKVTLKINEARSPVRLERIAREKFDMKKPRPEQVIKL"
     gene            complement(491..1384)
                     /gene="rsmH"
                     /locus_tag="PLH57_07980"
     CDS             complement(491..1384)
                     /gene="rsmH"
                     /locus_tag="PLH57_07980"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRK02591.1"
                     /translation="MSNKHVPVLPKECIEGLSPALQKAGAIFVDGTLGGAGHTRLFLE
                     HFPGLTVLACDIDANAITDARRSLESILAQHKIVFYHGSFSELAAGTNAPIPPPWDAV
                     LVDLGYSSNQLESADYGMSFQIDAPLDMRLRRPSHGPTAWEMIEHCSQQELADIFFRY
                     AEIRESRSLARAIKTALAQGELVNSTRRFAEFVAEKLHDKKRDIHPATTVFQALRIAV
                     NDELRTLDTFLKHVMLNLKKGGRLAVITFHSLEDRIVKSWGNSDQGVLPIYKKPVTAT
                     EEEIKSNPRSRSAKLRIYEKK"
     gene            complement(1381..>2381)
                     /locus_tag="PLH57_07985"
     CDS             complement(1381..>2381)
                     /locus_tag="PLH57_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRK02592.1"
                     /translation="HAHVPYASAVALDPATGRVLALVSHGSSTENLALRATFPAASIF
                     KIVTAAAAIQDGKLQYDSMIPVLGSYHTLYKRNLFSGGGLEPQTAPRFARLISLRDAF
                     GKSVNSVFGKIGLFGVGGTNLRRMAEGFYFGPAIPFELAVDSSSAVVPDGGFELAESA
                     SGFTRQNLMSPLHGALIAATIANQGVMMTPSVIDRVRDSDSDVVYLHKPQELSQPISE
                     TTADQLAKLMNRTITSGTSRRSFRGLDRNPMMAGVYIGGKTGTLNGNDPPGRYDWFVG
                     FANRDGVRIAIATLCIHGKYYGVKASTVARQILESFYEAIVTAEGETKSTAPSKRS"
BASE COUNT          593 a          576 c          605 g          607 t
ORIGIN      
        1 cggggcgtat aaaaagagac cgtaccttta gcgtgacagc taaaccctct ccgttgcgat
       61 ctaaaataag ccctcgccgt gggagagagg taagcttgga gttaaattgt cgtttcgaga
      121 ggcgttctag tttttcatca aatgcaaatt gaagataaac tgtccgggta acgataccgg
      181 cagcaaaaac aaaaaagaaa aagccgagcc accgaattcg gttcataact ttataacctg
      241 ttccggtcgt ggttttttca tgtcaaattt ttcgcgagcg attcgctcga gtcgcacggg
      301 agatcgggct tcgtttattt ttagcgttac tttatttacg tcgtcgcgag cctgttgttc
      361 taacttttcc aggcggtgga tatcgtagct gattcgaact atttcaagac ggatccacgc
      421 gaaactaccc atgaatgcgg ctcccgctgc gataaccaga aatattttta gctttggact
      481 cagcgaacga ctattttttt tcataaattc tgagttttgc actacgggat ctgggattac
      541 ttttaatttc ttcctcggtc gcggtgaccg gctttttgta gatgggcaac acaccttggt
      601 cagaattgcc ccagcttttc acgattcggt cttccagaga gtgaaacgtg atcacggcaa
      661 gtcggccccc cttttttagg ttcagcataa catgtttcaa aaaggtatcg agtgttcgta
      721 actcgtcgtt tactgcgatg cgtaacgctt gaaacacggt ggtcgcgggg tgaatatctc
      781 gctttttatc gtgcaatttt tcggcaacaa attcggcgaa cctacgagtg gaattgacca
      841 attctccttg ggccagcgct gttttaatgg cacgggctaa agaccgagat tctcgaatct
      901 cagcatagcg aaaaaagata tctgccagtt cctgttgtga gcaatgttca atcatttccc
      961 aggcagtcgg cccgtggctt gggcggcgta atcgcatgtc gaggggagcg tcgatttgaa
     1021 aactcattcc gtagtcagcg ctttcaagtt gattagacga gtatccgaga tccaccagaa
     1081 cggcgtccca cggaggtggg atgggtgcgt tagtgccggc cgcaagttcc gaaaaactac
     1141 cgtgataaaa gacgatcttg tgttgggcca aaatagattc cagcgaacgt cgcgcgtcgg
     1201 ttatggcgtt cgcgtcgata tcgcaggcta gtacggtgag accaggaaag tgttcgagaa
     1261 aaagtcgagt gtgacccgct cctccaagtg tgccgtccac aaatattgca ccagccttct
     1321 gaagtgcggg acttagccct tcaatgcact ctttaggtaa aaccggaacg tgtttgttgc
     1381 tcatgatctt ttggagggag cggtcgactt cgtctcgcct tcggccgtta cgatagcttc
     1441 gtaaaagctc tctaaaattt ggcgggcgac ggtagaagct ttaacaccat agtattttcc
     1501 gtgaatgcaa agcgtggcga ttgcgatgcg cacaccgtcg cgattagcga aaccaacgaa
     1561 ccaatcataa cgccccggtg ggtcgttccc attaagagtg ccagttttgc caccgatata
     1621 aacgccggcc atcatgggat tacgatcaag gccgcgaaac gaacggcgcg aggtgcccga
     1681 cgtaattgtg cgattcatga gtttagccaa ttgatctgca gtagtttcgc taatgggctg
     1741 cgatagttcc tgaggtttgt gcaagtaaac cacgtcggaa tcggaatcgc gaacccgatc
     1801 aataacggaa ggcgtcatca tgacgccctg attcgcaatg gtcgctgcta tcagcgctcc
     1861 gtgtaaggga ctcataagat tttgtctggt gaatcccgaa gcactttcag ccaattcaaa
     1921 tcccccgtcc ggtacgaccg ccgaactgga atcaactgct aattcaaagg gtatggctgg
     1981 gccgaagtaa aacccttccg ccatacgacg cagattggta ccgcccactc caaagagccc
     2041 tattttgcca aataccgaat tgactgattt gccaaaagca tctcgtagcg aaatcaaacg
     2101 agcaaagcgc ggcgccgttt gcggctctaa acctccacca ctaaacaaat tgcgtttata
     2161 gagggtgtga taacttccaa gcacaggaat catactgtcg tactgaagtt ttccgtcttg
     2221 tatggcggcc gcagcagtaa caattttaaa gatacttgcg gcgggaaaag tggcccgtaa
     2281 tgccagattt tcggtacttg agccgtggct tacgagtgcc aacactcgac cggtcgcagg
     2341 atctagcgct acggccgacg cgtacggcac atgggcgtgt t
//
LOCUS       DAOMXO010000369         2380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25823_length_2380_cov_3.873978, whole genome shotgun sequence.
ACCESSION   DAOMXO010000369 DAOMXO010000000
VERSION     DAOMXO010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25823_length_2380_cov_3.873978"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1112)
                     /locus_tag="PLH57_07990"
     CDS             complement(99..1112)
                     /locus_tag="PLH57_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02593.1"
                     /translation="MNRRLFSFWLVTASISLVAVSHGALADGRIKKGNKVTHRSRSSG
                     PDEKNFCHATDPRCINTLLRLLSGASLPVVETTVGYSSDSTASDSSVTGGVTVEAGSI
                     EMPIGDASLGRFEALFLSRDRGVRLRLTLGESNVMFECKTREGKTNIPGLGRMNHFCQ
                     PRRNKGVGGKLLELQYDPKKGRFAGRWAQFNAVLDLLGSSHSQTYLQRQLNLSAGLDA
                     ESIWHGDGSDSDHFSRLQLGLAGLVRSADARWEASAQIGYRPVVFGTLDALTDYGLTS
                     EGALVYNFLKNESAVVSTGLQFNAEYWSNPESSLGVLSSIEDRTSIYIGAVLGVRYEY
                     SRF"
     gene            complement(1453..1529)
                     /locus_tag="PLH57_07995"
     tRNA            complement(1453..1529)
                     /locus_tag="PLH57_07995"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1493..1495),aa:Met,seq:cat)
     gene            1628..>2380
                     /gene="tilS"
                     /locus_tag="PLH57_08000"
     CDS             1628..>2380
                     /gene="tilS"
                     /locus_tag="PLH57_08000"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRK02594.1"
                     /translation="MKTLKTIGTSQKLAATVAVSGGVDSLVLLHWVAFHFGNQIDLYP
                     VYVDHGQRADTERDWACVRTLCSRLPHLKSPTRIRIPKRDLLGSASEAQLRSLRYGVL
                     ERFSVSRNAPVVFTAHHADDVLETYLLKLLRGSHPTSLKGMAPKSRKGALWLVRPFLK
                     LHKSELVQYALANRIKWHEDATNSSPKYARNRIRNELLPLIESLRPKSTKKLLTFFSD
                     LEKLSSTLEEARTPSLGGVDQLSSPNGAPSLQL"
BASE COUNT          616 a          660 c          549 g          555 t
ORIGIN      
        1 aatttggcaa atgtcgaagc tgaaaaagcc gatcagaaaa ttgatcgact tctttcgaaa
       61 atccaaagca cggccacaaa agcacggcta atacggcact aaaagcgcga atactcatag
      121 cgcacaccca atacagctcc tatgtaaatg gaagtacgat cttcgatcga actcaatacc
      181 cctagcgaac tctcgggatt cgaccaatat tcggcgttaa actgcaagcc agtcgaaacc
      241 accgcggatt cgttttttaa aaaattataa accaatgcgc cctctgaggt taacccgtag
      301 tctgtcaacg catcgagagt accaaaaact actggtcgat agccgatctg cgcactagcc
      361 tcccatcgtg catcggcgga gcgcaccaat ccggcgagcc ccaattggag tcgactgaag
      421 tgatctgaat cactgccgtc gccgtgccaa atactttcgg catctagacc agcactcaag
      481 ttaagctgtc gctgaagata tgtctgtgaa tgggacgaac ctaacaaatc aagaacggca
      541 ttaaactgag cccagcgacc cgcgaatcgt ccttttttgg gatcgtattg aagctccaat
      601 aatttaccgc ccactccttt attccgtctg ggttggcaaa aatggttcat gcgaccgaga
      661 ccaggaatat tggttttacc ttctcgagtt ttacattcaa acataacgtt tgactcacca
      721 agcgtcagtc gcaaacgcac gccgcgatct cgcgacaaaa atagtgcttc aaaacgcccg
      781 agactcgcat cgccgattgg catttcaata gatcccgctt caactgtgac tccgcctgtg
      841 actgaagaat ctgaagctgt cgaatcactc gagtacccaa cggttgtctc gacaaccgga
      901 agactcgctc ctgagagcaa acgcaatagt gtgttgatgc agcgggggtc ggtggcgtgg
      961 caaaagtttt tctcgtcggg cccacttgaa cgactacgat gggtgacctt gttacccttt
     1021 tttatgcggc catcggctag tgccccatga gataccgcta ccaacgaaat cgaggccgtc
     1081 accagccaaa agctaaataa acgtcgattc aattaaatcc cccccactca atacgcaaaa
     1141 aaacgctagc accggtcttg gggggtgtct agatgggggg agttatcaaa ggctcacgtc
     1201 cgcggcggcg cattggttct ctgattttct ttggtttttt ggtggctgag ggcggagtcg
     1261 acctactaca ccttcgcgcc tcacgcgctc aggctgcgta ggctctcctc ggtcgctcag
     1321 caaaacatcc tgttttgctg ccccgcgcgc agtcgctttg cgactacccg caggtcgctt
     1381 cctcgcgttc gactccgctt caaagtttaa cgacccagcg gcgcattggt tctctgattt
     1441 tctttggttt tttggtggct gagggcggag tcgaaccgcc gacctacggg ttatgaatcc
     1501 gtcgctctca ccaactgagc tacccagcca atcctggtga aattaaagag cctacggtga
     1561 taacggagaa tagtacgaaa ttcaacatcg tttgacgcat tcgttaaagt agagcagtct
     1621 gccctcgatg aaaacactca aaacaatcgg taccagtcag aaattggcgg caacagttgc
     1681 tgtgtccggt ggggtggact ccctcgttct gctccattgg gtggcgttcc actttgggaa
     1741 tcaaatcgat ctttacccgg tttacgtgga ccacggacag agagccgata ctgagcgaga
     1801 ctgggcctgt gtacgcacgt tgtgctccag gttgccgcac ctaaaatcgc cgactcgtat
     1861 acgtattccc aaacgagatt tgctgggatc ggcatccgag gcccaactac gaagcctacg
     1921 ttacggtgtt ctagaacggt tttcagtatc acgaaacgcg ccagtggttt tcaccgctca
     1981 tcacgcagac gacgtgctcg aaacttactt actcaaactg ctaagaggat cgcacccaac
     2041 ttcgctgaaa ggaatggctc ctaaatctcg taagggagcc ctgtggctcg ttcgaccatt
     2101 tctgaaactt cataaatccg aactagttca atacgcgcta gctaatcgaa tcaaatggca
     2161 cgaagacgcc accaattcgt cacccaaata cgcccgcaac cgaattcgca acgaattatt
     2221 accgttgatc gaatcacttc gtccaaagtc gactaaaaaa cttttaacgt ttttctcgga
     2281 cctcgaaaaa ctttcgtcca cactcgaaga ggcccgcaca ccaagccttg gcggggtcga
     2341 ccaattaagc tccccaaacg gagccccgtc actgcaactt
//
LOCUS       DAOMXO010000370         2372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_25950_length_2372_cov_4.084161, whole genome shotgun sequence.
ACCESSION   DAOMXO010000370 DAOMXO010000000
VERSION     DAOMXO010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25950_length_2372_cov_4.084161"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2211
                     /locus_tag="PLH57_08005"
     CDS             <1..2211
                     /locus_tag="PLH57_08005"
                     /inference="COORDINATES: protein motif:HMM:NF018504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HRK02595.1"
                     /translation="KGGLRRMDPILSRIEQDVKTEFEKNNRILTFDEYISLVAKDARK
                     HTRGSAQYMIDMLDHFGTTTSGEERHFRLFDMEFDDPKFRVIAQDEVQQEIYRSLQNF
                     VKEGINNKLILLHGPNGSAKSSIVQCIMKGLEHYSETPDGAMYRFNWIFPVDRAVKGG
                     LGLGTYSPAKGALESYAKLPEEDVSARICCELKDHPLLLLPATARERLIREYGFKGNL
                     PDYLAKGSLCQKCKQIFEALLTAHKGDLKKVLAHVQIERFYISKRYRTAAVTIEPQLH
                     IDAQSRQITMDRSLSFLPASLQSLSLHELSGDLIDGNRGVVEYSDLLKRPVDTFKYLL
                     IACEMGSVNVQSSIAYLDTVLIGSTNELQLDAFKEFPDFTSFKARIELIRVPYLMSYS
                     RERKIYEVSLQKIAGSKHVAPHTTLVAGLWAVLSRLKKPNSIHYPPNVTNLIGSLSPI
                     EKARLYDHGGVPHNLSPEEKKLLRSSLKRLHEEYNAVPYYEGRMGASAREIKGILFDA
                     AQNSEFSCLSPLSALRELEDFVKRVSEYEFLKQDIKDGYHDCHEFITTAKNEYIQILD
                     QEVRESMGVFESRQYGDFLKKYVIHLSHLLKHEKIKNSITGKMESPDLTLIEQFETII
                     GAPSTAAERDTFRNNVISAIGAYALDHPNQPVEYKLVFPDFMQKLEDHYFNQQKSQMT
                     KLSDAIALFGTEKEDTTADHHKLARITVSNMCSKFGYCDQCAPGVISFLLKSKY"
BASE COUNT          677 a          582 c          521 g          592 t
ORIGIN      
        1 aaggggggtc ttagacgaat ggatccaatt ctttcgcgca ttgaacaaga cgttaagacc
       61 gagtttgaaa aaaacaaccg catccttacg ttcgacgaat acatttcttt ggtagccaaa
      121 gacgctcgaa aacacacacg cgggtcggcc caatacatga tcgatatgct ggatcacttt
      181 ggaaccacca ccagcgggga agaacgtcat tttcggctct tcgatatgga gtttgatgac
      241 cctaaatttc gggtaatcgc acaggacgaa gtgcaacaag aaatctaccg ttcgcttcag
      301 aacttcgtta aggaaggcat taacaacaag ttgattctac ttcacggacc aaatggttcg
      361 gccaagtcga gcattgttca atgcatcatg aagggactcg aacattacag tgaaacgccc
      421 gatggtgcga tgtaccgttt taactggatt ttcccagtcg accgcgcggt aaaaggcggt
      481 ctgggcctag gaacctattc gccagccaaa ggagctctag agtcttacgc gaaattgcca
      541 gaggaagacg tttctgcccg aatttgttgc gagttgaagg atcacccgct cttgctgttg
      601 cccgccacag cgcgcgaacg attgattcgc gaatatggct tcaaaggcaa tctgccggac
      661 tacctcgcaa agggttctct gtgccaaaaa tgtaaacaaa ttttcgaggc cctgctcact
      721 gcccacaaag gggacctcaa aaaggttctt gcccacgttc aaatagaacg cttttacatt
      781 tccaagcgct atcgcactgc cgctgtcacg attgaaccgc aactacacat tgatgctcaa
      841 tcacgccaga tcaccatgga tcgcagtcta agttttctgc ctgcctcgct ccaaagcctt
      901 tctctgcacg aactttcggg tgatctaata gacggcaacc gcggcgtagt tgagtattcc
      961 gacctactca aacgcccagt cgataccttt aaatatcttt taattgcctg tgaaatgggc
     1021 agcgtaaatg tacagtcgag catcgcctat ctcgacactg tgttgattgg ttcaactaat
     1081 gaactacaac tcgacgcttt caaggaattt ccggatttta cttcgttcaa agcgcgcatt
     1141 gaattaattc gagtgcccta tttaatgtcc tattcacgtg aacgcaaaat ctacgaggtc
     1201 agcctacaga aaattgccgg ctcgaaacac gtagcgccac acaccacatt agtcgcaggg
     1261 ctttgggccg tgctttcacg attgaaaaaa ccaaactcga ttcattaccc accaaacgtg
     1321 acgaatttaa ttggaagcct cagtccgatt gaaaaagccc gtctgtacga ccacggcgga
     1381 gttccccata atttgtcgcc agaagaaaag aaattattgc gatcttcgct aaaacgtttg
     1441 cacgaagaat acaatgcagt cccctattac gaagggcgca tgggcgcctc ggctcgcgag
     1501 attaagggca tactgtttga cgctgctcaa aattcagagt ttagctgtct cagtccgctt
     1561 tcggcactgc gcgagttgga ggattttgtt aaacgcgtga gcgaatacga attcctcaaa
     1621 caagatatta aagacggata ccacgactgc cacgaattca tcacaaccgc taaaaacgaa
     1681 tacattcaga ttctcgatca agaggtgcgc gagtccatgg gcgtgttcga gagtcggcaa
     1741 tacggagact ttttaaagaa gtacgttatt catttgtcgc atttgctgaa acacgaaaag
     1801 ataaagaatt cgattactgg gaaaatggaa tcaccggacc ttactttgat cgagcaattt
     1861 gaaaccatta tcggtgcccc aagcaccgca gctgagcggg acacctttcg taacaacgtt
     1921 atttcggcca tcggcgccta cgcgctcgat catccaaatc agccggttga gtacaagctt
     1981 gtttttccag attttatgca aaaactcgaa gaccactact ttaatcagca aaaatcccaa
     2041 atgaccaaac tcagtgacgc tatcgcgctc tttggaactg aaaaagaaga cacaacggct
     2101 gaccaccata aactcgcccg aatcacggta tcgaacatgt gttcgaaatt cggttattgt
     2161 gaccagtgcg cgcccggggt tattagcttc ttattaaagt cgaaatacta ggcactcatg
     2221 taaataattg aaaatactga tattttcaaa ttttgacctc attagtcaag catgttataa
     2281 cgcgcccgtg cattgcatta gatttatcgc tatttggaca ttgcttttgc tttcgttggt
     2341 accgaacaat tcgtgggcaa ccaaagagca ga
//
LOCUS       DAOMXO010000371         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26024_length_2367_cov_5.602076, whole genome shotgun sequence.
ACCESSION   DAOMXO010000371 DAOMXO010000000
VERSION     DAOMXO010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26024_length_2367_cov_5.602076"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLH57_08010"
     CDS             <1..608
                     /locus_tag="PLH57_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK02596.1"
                     /translation="KSGMGKSVTLKTTIGLMKPDRGEIYVHGEAVHTASEHTLAQVRK
                     KCGMVFQHPALLDSLTIFENVAFGLETKNRERVIECLKLVHLDESILNRKPQEISYGM
                     QKRVSLARTLAPGTQILLFDEPTTGLDPITTNAVNELIAELSDRLNVTSVVVSHDMGC
                     ALRIADRILVLDQGNILALDTVDRIKSSSEPLIQDFLREAL"
     gene            610..1398
                     /locus_tag="PLH57_08015"
     CDS             610..1398
                     /locus_tag="PLH57_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK02597.1"
                     /translation="MQLVLGFFADIGGMALFSLEVVRTWLRTFGQNGSLIVEQIALVA
                     YRSLVTILFSGFFVGAILVIQFHLMLSKYDAVTLLGGLNTSATIREVGPLIISFLLAG
                     KVGAYTAAELGTMRVTEQIDAIRCLGRDPVQYLIVPRFIAIVLCSLLLLVFGVLVGVA
                     GSMVVAVELYNMNFLQYMESIPRFTGIWTIIAGVFKSLVYGLIVAAVSTYKGYFASGG
                     AQGVGRAVTECAVYTNLLIVFANSFTSYFLENVQQFVNNTFGGF"
     gene            1401..2165
                     /locus_tag="PLH57_08020"
     CDS             1401..2165
                     /locus_tag="PLH57_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK02598.1"
                     /translation="MKKQLFYFVTAVHDVFFIFGKTLLTCLKGPHRAKEIFHHINQIG
                     IGSLPVILLSTAFAGFVITGEIAFHMDEALHTVEMIPGFSAQFILREIGVVIPALLMV
                     SKAGAANTAEVGSMKVTEQIDALRLLQIDPIEYLVYPRFVASIVSLACLTLISIFVTL
                     SCATAIAVIRYHFTWLEYVNSLLVFVTPRDLISALLKGLVFGAVIPVISCFYGFRCTG
                     GAEGVGSATTNSVVTSTILIILLDFVLTFAFSVLLY"
BASE COUNT          539 a          553 c          580 g          695 t
ORIGIN      
        1 gcaagtccgg tatggggaaa tcggttacct taaaaacgac tattggctta atgaaacccg
       61 atcgaggcga aatctacgtt cacggcgaag ccgtgcacac agcaagtgag cacactctcg
      121 cccaagtacg aaaaaaatgt ggcatggtat tccaacatcc ggcgcttcta gattcgctga
      181 ctatttttga aaacgtggcc tttggactcg aaactaaaaa tcgagaacgg gttattgaat
      241 gcttaaagtt agtgcacttg gacgaaagta ttttaaatcg taaaccccaa gaaattagct
      301 acggcatgca aaaaagagtc agtcttgccc gcactctcgc tccaggcacc cagattctat
      361 tattcgatga acctacgact ggcctggatc cgattactac caacgcagtg aacgaactta
      421 tcgcggaact ttctgatcga ttgaacgtca ctagcgtagt ggtttcgcac gacatgggct
      481 gcgcgcttcg aatcgcagat cgcatattgg tcttggacca gggcaacatt ctcgcgctcg
      541 atactgtaga ccggatcaaa tcaagtagcg aacctttgat tcaagacttc ctacgggagg
      601 cactctaaaa tgcagctcgt attaggcttc tttgcagata ttggaggaat ggcgttattc
      661 tcgctcgagg tggttcgcac ctggcttcgc acgtttggcc agaacggttc gctcattgtt
      721 gaacaaatcg ccctcgttgc ctatcgctcg ctggtgacga tcttgttttc gggtttcttt
      781 gtcggtgcga ttttggtgat tcaatttcac ctaatgttgt caaagtacga cgctgttacg
      841 ctcttgggtg gcctaaacac ttcggcgacc attcgcgaag tgggcccgct tatcatttcg
      901 tttcttctcg ctggtaaggt gggcgcctat acggctgctg aactgggcac catgcgggtg
      961 acggaacaaa tcgacgcaat acgttgcctt ggacgcgacc ccgtgcagta tttgatagtg
     1021 ccaagattta tcgcgatagt gctttgctcg ctcctacttt tggtattcgg ggtattagtt
     1081 ggcgtagccg gtagcatggt agtggccgta gaactctaca acatgaattt cctacaatac
     1141 atggagtcga tcccacggtt caccggcatt tggactatca tagccggcgt ttttaaatcc
     1201 ctggtgtacg gactaattgt tgcagccgtc agtacctaca agggttattt cgcctctggc
     1261 ggcgctcaag gggttgggcg cgcggtcaca gagtgcgcag tttataccaa tttattaata
     1321 gtttttgcca attccttcac ttcctatttt ctcgaaaacg tccaacagtt tgtgaacaac
     1381 acgtttgggg gattttagag atgaagaaac aactctttta tttcgtaacc gccgttcatg
     1441 acgtgttctt tattttcgga aaaactcttc ttacttgttt gaaagggccc caccgcgcga
     1501 aagagatttt tcatcacatt aatcagatcg gaataggatc attgccggtg attttacttt
     1561 cgacggcatt cgctggattc gtgatcacag gagaaatcgc ttttcacatg gacgaggcgt
     1621 tgcatacggt tgaaatgatt ccggggtttt ccgcacagtt tatcctacgg gaaatcggcg
     1681 tggttatccc agcgctttta atggtttcta aggcgggagc ggcaaatacc gcggaagtcg
     1741 gttcaatgaa ggtgaccgaa caaatcgacg ctttacgtct actgcaaatc gaccccatcg
     1801 aatatttggt ctaccctcgt ttcgtcgcct cgatcgtgtc gcttgcgtgt ttgactctaa
     1861 tctcaatctt cgtcacccta agctgtgcca ccgcaatcgc ggtgattcgc taccacttta
     1921 cctggctaga gtacgtgaat tcgctactgg tattcgtaac accgcgtgac cttatctctg
     1981 cactattgaa agggctcgta ttcggggcgg tcattccggt catttcctgt ttttatggtt
     2041 ttcgttgcac tggtggtgcc gaaggagtgg gatcggctac tacgaactct gtggttactt
     2101 cgacgatttt gatcatactt ttggactttg ttctcacatt tgcgttttcg gttcttctat
     2161 attaaccgtt ttttactgag gttacgggcc tatgaaattt tccaatgaag cgaaagtggg
     2221 cttactattg tttgccacat tggtgcttac actagtgttc gcctggcttt tgggtgcgaa
     2281 taatccgttt cgccgtaatg tagagatata cgtaacctat aactttgcgg gtggcattga
     2341 tatcggcagt cccgttcgag tggcggg
//
LOCUS       DAOMXO010000372         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26032_length_2367_cov_3.737024, whole genome shotgun sequence.
ACCESSION   DAOMXO010000372 DAOMXO010000000
VERSION     DAOMXO010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26032_length_2367_cov_3.737024"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..206
                     /locus_tag="PLH57_08025"
     CDS             60..206
                     /locus_tag="PLH57_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02599.1"
                     /translation="MLHGRRTGKKFKIGDRLQVVVLRVDMDNGFVDFSLDTENKIIED
                     AAKD"
     gene            208..1893
                     /locus_tag="PLH57_08030"
     CDS             208..1893
                     /locus_tag="PLH57_08030"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRK02600.1"
                     /translation="MQIFRTGKQVTQAVKNVQRLRAILGVFGRHGLQEFLIRMGLERH
                     LSASAKEEGVENRTLPERLRMAFEHLGPTFVKLGQLLSNRPDMIPENFIDEFKKLQDN
                     VAPLPFEAIRTQIEKELGGKISDFFSEIHAEPLASASIGQVHAAKLLDGSDVVVKVQR
                     PDIDHTIKQDISILSFLAAMMEKYLPETKFLSPQQIVEEFSRALHYELDFVIEANTMI
                     RFAENARGNSKVVIPKVFRKLSGKRVLTMERLHGARITDLAAVKAMGINPRDLVDIGA
                     RAFFKNVMIDGLFHGDLHGGNVFAMKNPETGEAQIGVIDFGIVGRLSQKSRDSFSRMV
                     LALVSEDYETLCYEYAELGASGTGIDFDAFQREVRNTLSPYVGAALKDVNAGAILIEA
                     TKIAVRYSIKIPGDWMVVFKAIFTLEGMGRQLDPDFDLLAIGSDLVKTVLKDRYSFQR
                     ITKDFAWLARDVNALLQVAPRQIRWMFRKFNANDFAFEIKFKEVEVIRRQMERSFRGL
                     GLAILTAALFMAAAMSLQMQSGHFVFTYYPLPSILFFSMGLIGFLGLILRSWR"
     gene            complement(1890..>2367)
                     /locus_tag="PLH57_08035"
     CDS             complement(1890..>2367)
                     /locus_tag="PLH57_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02601.1"
                     /translation="RRHTPVRPALPVQRVLPHYLQPSLIRDRLSGRVFRCERTPEYWA
                     ELEKIRTCTRLQWLNEQGEATAYLAYNRGKDLKGVVHEWGGEGNALTKLAAVAASKNS
                     DLTWLTHPGLADPIMGLAENRTSLKPLMQSHLALFKPLTSRVIDYSKFWFWGLDSL"
BASE COUNT          627 a          514 c          598 g          628 t
ORIGIN      
        1 cataagttta tcttcgataa attcctggcg actactatga atttatcgaa gataaactta
       61 tgttgcacgg cagaagaact ggaaaaaaat tcaaaattgg agatcgactt caggttgtgg
      121 ttttaagggt cgacatggat aatggatttg ttgatttttc attggatacc gaaaacaaaa
      181 taatcgaaga cgcagcgaaa gactaagatg caaatttttc gaaccggaaa acaagtgacc
      241 caagcagtaa aaaacgtaca acgtctacgg gcgattttag gagtatttgg tcgacacggg
      301 cttcaggaat ttttaattcg aatgggcctt gaacgccacc tttctgcttc cgcgaaggaa
      361 gagggcgttg aaaatcggac gttaccagaa cgactgcgca tggcgtttga gcacctcggc
      421 ccaacgttcg taaaactcgg acagttgctt agcaatcggc ccgatatgat tcccgaaaac
      481 ttcattgacg agtttaaaaa attacaggac aacgtcgcgc ctttgccgtt tgaggctata
      541 cgcacacaaa tagaaaaaga gcttggcggc aagatcagtg attttttttc ggaaatacat
      601 gcggagcccc tcgcctctgc tagcatcggg caagttcatg cggcgaagtt gttagatggt
      661 tctgacgtag tagtgaaggt acagcgaccc gacattgacc acactataaa acaagatatt
      721 tcgatcttgt catttctcgc ggcaatgatg gaaaaatact tgcccgagac caagtttttg
      781 agcccacaac aaattgtaga agaattttcc agagcccttc attacgaact agatttcgta
      841 attgaagcca atacgatgat tcgtttcgcc gagaacgccc gtgggaattc aaaggtcgtg
      901 attccgaaag tatttcgcaa gctatcgggc aaacgagtgc tcaccatgga gcgtttacat
      961 ggagcgcgga ttaccgacct cgcggcggta aaagcgatgg gaattaatcc acgtgatttg
     1021 gtcgatatcg gagctcgagc tttttttaaa aacgtaatga tcgatggttt atttcacgga
     1081 gatcttcacg gcggtaacgt gtttgcgatg aaaaaccccg aaaccggtga ggcgcaaata
     1141 ggtgttatcg atttcggaat cgtcggacga ttgagtcaga agtctagaga ctctttttcg
     1201 cgaatggtct tagcgctagt aagcgaagac tatgaaactc tgtgttacga atacgcagaa
     1261 ttaggcgcta gcggaacagg tattgatttt gatgcctttc aaagagaagt acgaaatacg
     1321 ctatcgccct atgttggagc ggcactaaaa gatgtaaacg ctggggctat tttaatagag
     1381 gccacaaaaa tagcagttcg gtatagcatt aaaattcctg gcgattggat ggtcgttttc
     1441 aaggcgatat ttacgcttga gggcatgggg cggcagttag accctgattt cgatttactg
     1501 gccattggtt cagatcttgt aaaaaccgta ttgaaagacc gctactcatt tcaacgcatt
     1561 accaaagatt ttgcttggct cgctcgcgac gtaaacgcac tcttgcaagt agccccccga
     1621 caaattcgtt ggatgttccg taaattcaac gccaatgact ttgcttttga aattaagttc
     1681 aaggaagtcg aagtgattcg gcgtcagatg gaacggagtt ttcgtggttt ggggctcgcc
     1741 attttgacag cagcgctttt catggcggct gctatgagtt tacaaatgca gtccggccac
     1801 tttgttttta cctactaccc gcttccctcg attttatttt tctcgatggg cttaattggg
     1861 tttttaggac ttattttgcg aagttggcgt taaagtgagt cgagtcccca gaaccagaac
     1921 tttgaataat caatgacccg acttgttagt ggcttgaaca acgccaaatg cgattgcatt
     1981 agtggcttga gcgaggttcg attttccgct agtcccatga tggggtcagc caaaccgggg
     2041 tgagtgagcc acgtgaggtc cgagtttttg ctcgcagcta ctgccgccaa tttcgtgagc
     2101 gcgttaccct cgccccccca ttcgtgcacg acgcctttca agtccttgcc acggttgtag
     2161 gccaggtaag ccgtagcttc gccttgttca tttaaccact gcaaccgagt acaggtcctg
     2221 attttttcga gctccgccca atattctggg gtccgttcgc agcgaaacac gcggccagag
     2281 aggcgatcgc gtatcaggct tggctggagg taatgcggaa gtaccctctg gacgggtagt
     2341 gcaggccgga caggagtgtg acggcgc
//
LOCUS       DAOMXO010000373         2364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26069_length_2364_cov_5.269381, whole genome shotgun sequence.
ACCESSION   DAOMXO010000373 DAOMXO010000000
VERSION     DAOMXO010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26069_length_2364_cov_5.269381"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLH57_08040"
     CDS             <1..208
                     /locus_tag="PLH57_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02602.1"
                     /translation="FLEKNAGGEDPLLDAKMDLIKNLPPVEALRETGAVDNSEIQTAS
                     DAIKEDKEEASSDLPPSSGVKKKP"
     gene            205..1923
                     /gene="gspE"
                     /locus_tag="PLH57_08045"
     CDS             205..1923
                     /gene="gspE"
                     /locus_tag="PLH57_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HRK02603.1"
                     /translation="MSKPRDPTELESESRIGQLLLKNTTLSPTQLEEALEIQKREGGV
                     LGEILVRRNFVLQHEILRALCAQLDLPYVEDLKPNDVDATLVKDLPINYAKTKEVLPI
                     SKLGDTLKVATSNPFNLSMLEDIRVFTGCRVQLVVSTPARIQDAINRVYERAQANMMT
                     DIKNEFDETYDLEGPIDILDASEDDAPVIKFVNSMVFRAVKERASDIHVEPFEKEMII
                     RFRIDGVLYDILRQPKRAHAAISSRIKVMGQLDIAEKRLPQDGRIKIKLAGKDIDIRL
                     STIPTAHGERCVMRILEQTDRVLELPQLGFSGDTLIHIESLIKKKHGIILVTGPTGHG
                     KSTTLSAALSKLNSPERCIITVEDPIEYVIHGVNQIQVNSKIDLTFARALRSILRQNP
                     DVIMIGEIRDRETAEIAVNASLTGHLVLSTLHTNDSSGAPTRLLDMGIEPFLIASSIL
                     GVVAQRLIRRVCTKCRQEYTPTDFELQELGITRENAKGGHFYKPVGCPNCAGSGYAGR
                     TVVHELLLIDDEVRQLIVKASDSAAIKKKAIEQGMRTLREDGVQKVMQGITTIEELLR
                     AIQADE"
     gene            1923..>2364
                     /locus_tag="PLH57_08050"
     CDS             1923..>2364
                     /locus_tag="PLH57_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRK02604.1"
                     /translation="MPIFEYKALDAAGKIIKGSIDAETAKAAKQKLKKNELFPTEIRE
                     KSAAKSKSTGRPSGGALFSRLKIQDIALATRQLASLVRANIPLVDALKALIEQTEHEK
                     LQVIFEEVRKDVNEGSSLAKALAKHPRAFNNVFINMVEAGETSGT"
BASE COUNT          695 a          533 c          581 g          555 t
ORIGIN      
        1 gtttcttgaa aagaatgcag gtggtgaaga tcctttactc gacgccaaaa tggatcttat
       61 caaaaacttg cccccggtag aggcactgcg ggaaactgga gcagtagata atagcgaaat
      121 tcaaacagcc agtgacgcaa ttaaagaaga taaagaagag gcttcttccg atcttccacc
      181 tagttccgga gtgaaaaaga aaccatgagt aaaccccgag acccgacaga actcgagtct
      241 gaaagtcgta taggtcagct attgctgaaa aatacgacgc tctctcctac gcagctagaa
      301 gaggcgctcg aaattcagaa acgcgaaggc ggcgtattgg gggaaatttt agtacgtaga
      361 aatttcgtat tgcagcacga aattctaaga gctctttgcg cgcaactgga tctgccctat
      421 gtcgaagatc taaaacccaa tgatgtagat gcgaccctcg taaaggatct tcccatcaac
      481 tacgcaaaga ctaaggaagt cctgcctata tctaagttgg gggacacgct caaggtcgct
      541 accagcaatc cgtttaattt gtcgatgtta gaagatattc gggttttcac tggttgccga
      601 gtgcaactcg tcgttagtac gcccgctcga attcaggacg caatcaatcg cgtatacgag
      661 cgcgctcagg ctaacatgat gaccgatatt aagaacgagt tcgacgaaac ctacgattta
      721 gaaggtccaa tcgacattct cgatgcgagt gaagacgacg caccagtaat taaattcgtg
      781 aactccatgg tgtttcgggc agtaaaagaa cgggcttcgg atatacatgt cgagccattt
      841 gaaaaagaaa tgatcattcg atttcgaatc gacggcgtac tgtatgacat tcttcgccaa
      901 ccgaaacgcg cgcatgctgc gatttcgtca cgcattaaag taatgggtca gctcgacatt
      961 gccgaaaaac gcctacccca agacggtcgt attaaaatta aacttgctgg taaagatatc
     1021 gatatacggt tgtcgacaat tcctaccgca cacggtgagc ggtgtgtaat gcgtattttg
     1081 gagcagactg accgcgtgtt agagctgcca cagttggggt tctcaggtga tacattaatt
     1141 catattgaaa gcctcattaa gaagaaacac ggaattattt tggtcaccgg tccgactgga
     1201 cacggtaagt cgaccactct ttccgctgcc ctaagtaagt taaatagtcc ggaacgttgc
     1261 atcattaccg tggaagatcc aatcgagtat gtcattcacg gggtgaatca gattcaggtg
     1321 aattctaaaa tcgatttgac gtttgctcga gcgctacgaa gtatcttgcg tcaaaatcca
     1381 gacgtaatca tgatcggtga aattcgcgac cgtgaaacag ccgaaattgc cgtaaatgct
     1441 tcgctgaccg gtcacttagt tttatctacc ttgcacacca atgattcatc gggtgctcct
     1501 actcgattac tcgacatggg aatcgaaccg tttttgattg catcttcaat actcggggtg
     1561 gtggcgcaac gattgattcg acgtgtttgt accaagtgtc gtcaggaata cacacccacc
     1621 gatttcgagt tgcaggaatt gggaattacc cgcgaaaacg ccaaaggtgg gcacttttat
     1681 aagccagtcg gatgccccaa ctgcgccggc agtggctacg cgggacgaac cgttgtgcac
     1741 gagctgttgc ttattgatga tgaagtgcgc caattaattg ttaaggcctc agactcagct
     1801 gccataaaaa agaaagctat tgagcaaggg atgcgaacgc tgcgcgaaga tggcgtacaa
     1861 aaggtcatgc agggaatcac tactatcgaa gaacttttac gtgcgataca ggcggatgaa
     1921 taatgcctat ttttgagtac aaggcgctcg acgcggccgg aaaaattata aaaggctcaa
     1981 tcgatgctga aacagcaaag gccgcgaagc agaaactaaa gaaaaacgaa ttgtttccca
     2041 cggagatacg agaaaagtcc gcagccaagt cgaaaagtac gggccgcccg agcggaggcg
     2101 ccctttttag tagattgaaa attcaagata ttgctttggc tactcggcag ttggcttcgc
     2161 ttgtacgcgc gaacattcca ctggtggacg ccttaaaagc actgatcgag caaaccgaac
     2221 acgaaaaact tcaggtaatt ttcgaagaag taagaaagga cgtcaacgag ggatcttcgt
     2281 tggcaaaagc tctcgccaag catccccgag cttttaacaa tgtatttatc aacatggtgg
     2341 aggcgggcga aacgtccggc actc
//
LOCUS       DAOMXO010000374         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26198_length_2356_cov_4.420252, whole genome shotgun sequence.
ACCESSION   DAOMXO010000374 DAOMXO010000000
VERSION     DAOMXO010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26198_length_2356_cov_4.420252"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLH57_08055"
     CDS             complement(<1..616)
                     /locus_tag="PLH57_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HRK02605.1"
                     /translation="MCGIAAIISPNPKPLSFDILQMANAIRHRGPDGEGFALISAGRD
                     PRFFSGADTVHESWSFEIPYRPTAEISKAGDLLSHVAFGHRRLSILDLSQTGHQPMFR
                     PEPGLVITYNGEIYNYLELRAELEKRGHRFSTGTDTEVLLCAYEEWGPDCLHRFNGMF
                     AFVLYDLKRNQVFAARDRFGVKPLYFWRSPDGVVAFASEIKQFTV"
     gene            723..1655
                     /locus_tag="PLH57_08060"
     CDS             723..1655
                     /locus_tag="PLH57_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006709261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK02606.1"
                     /translation="MKLIIQIPCLNEAKTLDVTLRDLPKSIPGIDQIETLVIDDGSTD
                     NTSETAKKLGVRHIVRHSINRGLAAAFQTGLRKALDEGADIVVNTDADNQYNGADIAK
                     LVEPIIKGHADMVVGIRPIGEISHFSWLKKRLQRLGSWVVRVLSGTDVRDATSGFRAY
                     TRDCAASIHLITRFSHTLETLIQMGNKNFVVDQVDVRVNEKLRESRLFRSMREYITRS
                     FFDIFRIYLFYKGDKVFAISGTCLFLPGFALGLRFLHIHFFLGEGGRVQSLILATLLM
                     ITGFQCFLVAILFAAQIGNRRLMEIALSRMNKAP"
     gene            1730..1806
                     /locus_tag="PLH57_08065"
     tRNA            1730..1806
                     /locus_tag="PLH57_08065"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1764..1766,aa:Pro,seq:ggg)
     gene            1877..>2356
                     /locus_tag="PLH57_08070"
     CDS             1877..>2356
                     /locus_tag="PLH57_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK02607.1"
                     /translation="MKNDDVFFSLVILCYRAEESILPFVKRVHSIMSLYNFNWEIVLV
                     ANYLPNSTDKTPSIVKKLSDEYPHIRCVSEEKQGMMGWDLRKGLHSTRGQYIAMIDGD
                     GQFPVESIFACFARMLMDDVDLVKTFRVFRYDGFYRTVISRGYNLIFRILFGKHFKDV
                     "
BASE COUNT          634 a          573 c          555 g          594 t
ORIGIN      
        1 acaccgtaaa ctgcttaatt tcggatgcaa acgcaacaac accatccggt gagcgccaga
       61 aataaagcgg tttcacgcca aaccggtccc gtgcggcaaa tacttgattg cgtttaaggt
      121 cgtagagcac aaaggcaaac attccgttga aacgatgaag gcaatcaggc ccccattcct
      181 cgtaggcaca gagaagcact tcggtgtcgg tgccggtaga aaagcgatgt ccacgctttt
      241 cgagttctgc acgcaactca aggtaattgt aaatttcacc gttgtaggta atcacaaggc
      301 ctggttcggg acgaaacata ggctgatggc cagtttggga aagatccaaa atactcaggc
      361 gccggtgccc gaaagctaca tgagataaaa gatcgcccgc cttagaaatt tcagctgttg
      421 gacgataggg tatttcgaaa ctccagctct catggaccgt gtcggcaccg ctaaaaaaac
      481 gcggatcccg gcccgccgaa atcaacgcga aaccttcacc gtcggggccc cgatgacgaa
      541 ttgcgttagc catctgcaag atatcaaaag agagtggttt aggatttggg ctaataatag
      601 cagcgatccc acacatgccc gccagtgtgc tccggtcggt actcaaagac aatactggaa
      661 attcagcggg ccaggaaacc ctagtcaaag cttagatttt taagttagaa tccatgggaa
      721 tcatgaagct tattattcaa attccctgcc tcaatgaagc caaaacgctg gacgtaactt
      781 tgcgtgacct tccgaagtcg attcccggga ttgatcaaat cgaaacccta gttattgacg
      841 atggtagtac cgataacacc tcggagaccg cgaaaaaatt gggggtaaga cacatcgtac
      901 ggcactccat taatagggga ctggccgccg cgtttcagac cggcctgcgg aaagctttgg
      961 acgaaggcgc cgacattgtg gtgaatactg acgccgacaa ccaatacaac ggcgcagata
     1021 tcgccaagtt ggtggaaccc ataattaaag ggcacgccga catggtggta ggtatacggc
     1081 ccattggcga aatttcccat tttagttggt taaaaaaacg acttcagaga ctgggaagct
     1141 gggtcgttcg ggtactttcg ggcaccgacg ttcgcgacgc cacctctggc tttagagcgt
     1201 atacccgcga ttgcgcagct tcgattcatt taattactcg attttcccac accctcgaaa
     1261 cactcattca aatgggcaat aaaaacttcg tagtggacca agtggatgtg cgcgtaaacg
     1321 aaaaactgag ggaatccaga ctatttcgtt caatgcgaga gtacatcact cggtcgttct
     1381 tcgatatatt tcgcatctat ctgttttaca aaggcgacaa agtttttgcg atttcaggga
     1441 catgcctgtt tttacccgga tttgcacttg gattgcgctt cctccacatc catttcttct
     1501 taggcgaagg tggccgagtt cagtctttga ttctggcaac attactcatg atcactggtt
     1561 ttcaatgttt tttagtcgct attctctttg cggcccagat cggaaatcgc agactgatgg
     1621 aaatagcgct ttcgagaatg aataaagcgc cctaatgctt acgtcttgac acaaattacg
     1681 ccaacccggt aaacgctata tggtctgtac cccattgctc actataggtc gggctgtagc
     1741 gcagcctggt agcgcacttc cttggggtgg aagtggtcgc tggttcaaat ccagtcagcc
     1801 cgaccatttg acacgccaag gccagcccgg gagttgacat accccaaagc acctgactac
     1861 atttctcacc aatcatatga aaaacgatga tgtatttttt tcgcttgtga tcttatgcta
     1921 tcgcgctgag gaatcaatcc tacccttcgt gaaacgcgtt cattcaatca tgtcattgta
     1981 caatttcaac tgggagattg ttctcgtcgc taactatctt ccgaactcga cagataaaac
     2041 tccatcaatc gtaaaaaagc tttccgatga atacccgcac attcgctgcg tctcggaaga
     2101 aaaacaaggc atgatggggt gggatctacg caagggtctc cactctacga ggggtcagta
     2161 catcgccatg atcgacggtg atggacaatt tccagtggag tccatttttg cctgctttgc
     2221 gcgcatgttg atggacgacg tagaccttgt aaaaacattt cgtgtgtttc gttatgatgg
     2281 attttaccgc actgtaattt cgcgcggata taatctaatt tttcgaattt tattcggcaa
     2341 acacttcaaa gatgta
//
LOCUS       DAOMXO010000375         2354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26236_length_2354_cov_4.403654, whole genome shotgun sequence.
ACCESSION   DAOMXO010000375 DAOMXO010000000
VERSION     DAOMXO010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26236_length_2354_cov_4.403654"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2354)
                     /locus_tag="PLH57_08075"
     CDS             complement(<1..>2354)
                     /locus_tag="PLH57_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02608.1"
                     /translation="ARRLNLLESNEDKQIYLYPDTENQHFRVDWHSNRLFIKKLGSSE
                     APTKLFDVQPDEMTFFAFPLDGSEEDDAREKKKARTYVALLSIKSGDRGRTHSFHVRD
                     LGKTLLVEQHVVLSDTFYSKAELGRRVVLAEDDRYIFDDVTPPQPSISAYDSVYESSG
                     LHIDILASHENKLARRLRRPIDEVELGPSLAVRLYGYGDEITPPSGVYTERKKGAPLI
                     AGQLLSRANESGEKKYFVRRTTVNLPGENGNKPLTFNVSAAIIDPSFGSGSRGYNVQI
                     FVTSPEESSVPFYAKESLSSPSNSGDGQLDQLEAIELRSGKGENSDKVFLIMAFRDVV
                     AHGKILRKGYIAVNPYTVSLPNKKDAERGRAKEVILSATTKPFVIPDMSISHKELNSR
                     LVFEASTGEPCWEMNPDLDTADANYEVLSFLSGKAWKPNLSENPNAKVERLKDDAYRK
                     GQSWKIHGRNSNRAEIQSLNKMDLSEYRQDVLPKITEVMAEMIEPGTPVGHRVFIVDK
                     TLKSFVVNTLLARYIDELDKAQPRRWNYRNGVPLYLFNPADIKEQSTVMENLEGIRDM
                     KGRPVLVVDLQHLSKTEINPTPGSDGFYVRETKAKKGTGPEAETGDVDVLSEQKVPSL
                     MYLLATEGQRIELVKFPKLSTGYRKISTLFIGTREEWASAKAKTPENKYGLFEKVEVI
                     DLGEPTAEMRKGLFRRSLEAPEIKALELQFDSSEIARHEKNLTNEEQLERILDYIVSR
                     SERLASEENRDIFNAFLRLRNQLVRSLLEDEYIRKNRVVDVRYV"
BASE COUNT          582 a          553 c          503 g          716 t
ORIGIN      
        1 ccacgtatcg aacatccact acccgatttt ttcgaatata ttcgtcttca agcagcgatc
       61 gaacaagttg attgcgcaac cggagaaagg cgttaaaaat gtcgcgattt tcttcggaag
      121 caagccgttc ggaacgagaa actatatagt ctagaattct ttctagttgc tcttcgttgg
      181 taagattttt ttcgtgtcga gcgatctcgg aactatcgaa ctgcagttct agtgctttaa
      241 tctctggggc ttcaagggaa cggcgaaaaa gtccttttct catttctgcg gttggttcac
      301 ctaaatcgat gacttcgact ttttcgaata gtccgtattt attttccgga gtcttggcct
      361 ttgccgaggc ccattcttct ctagtaccta tgaacagggt tgatatcttt cgatatccag
      421 tggacagttt cggaaacttt acgagttcga ttcgctgtcc ctcagtggcc aacaaataca
      481 ttaaacttgg aactttctgc tcagaaagca catctacgtc accggtttca gcttcaggtc
      541 ctgtcccctt tttagctttg gtctctcgca cataaaaccc atcactaccc ggcgtcggat
      601 taatctccgt tttcgagagg tgctgtaaat ctacgaccaa tacagggcga cctttcatat
      661 ctcgaatgcc ttcgagattt tccataacgg tcgactgttc ttttatatcc gcgggattaa
      721 ataaatatag tggcacaccg tttcggtaat tccaacgtcg tggctgtgct ttatctagtt
      781 catcaatgta gcgagcgaga agcgtattca cgacgaaaga ttttaatgtt ttatcgacga
      841 taaaaacgcg atgtcctacc ggagttcctg gttcgatcat ttcagccatg acttccgtaa
      901 ttttcggaag tacgtcttgt cgatattccg aaaggtccat tttatttaaa ctttggattt
      961 cggcccgatt cgaattgcgg ccatgaattt tccaggactg gccttttcgg taagcgtcgt
     1021 cttttaaacg ttccactttt gcatttggat tttctgataa atttggcttc cacgccttgc
     1081 cagaaagaaa acttaaaacc tcgtaattcg catctgccgt gtctaaatct ggattcattt
     1141 cccaacaggg ttctccagtg gaagcctcaa acactaatcg cgagttcagt tctttatgac
     1201 tgatggacat gtcaggaatg acaaacggtt tagtggttgc acttaaaatt acttctttag
     1261 cacgccctcg ctcggcatct tttttatttg gtagagatac tgtgtaaggg ttaaccgcga
     1321 tgtaaccttt acgcagaatt tttccgtgcg ccacaacgtc tcgaaacgcc atgattagaa
     1381 aaactttgtc ggaattttcg cctttgccgg aacgtaattc gatggcctcc aattgatcca
     1441 gctgcccgtc gccggagttt gagggactac tcaaactctc ttttgcataa aatggtactg
     1501 aagattcctc gggacttgtt acgaaaattt gaacgttgta acccctacta ccagagccaa
     1561 acgaagggtc aataattgcg gcactcacgt taaacgtaag aggtttattg ccattttcac
     1621 caggtaggtt caccgttgta cggcgcacga agtatttttt ctcgccactt tcgttggccc
     1681 tactaagtaa ttgccccgca ataaggggcg ctcctttttt acgttccgta tatactccgg
     1741 aaggcggagt tatttcatca ccgtatccgt agagacgaac cgctagagag gggcccaatt
     1801 ccacttcgtc aattggtcgt ctgagtcggc gagccagttt gttttcatgg ctcgcaagta
     1861 tatcgatatg aagcccactg ctttcatata ctgaatcgta agctgaaatt gagggttgcg
     1921 gaggggtaac gtcgtcaaag atatatcgat catcctctgc caacactact cgtcggccca
     1981 attccgcctt cgaataaaaa gtatcgctca aaacaacatg ttgctcgaca aggagcgttt
     2041 ttcctaaatc cctgacatga aacgaatggg tgcgacctct atcgccggac ttgatcgata
     2101 gcagcgcaac gtaggttcta gctttctttt tttcccgcgc atcgtcttct tctgaaccgt
     2161 ctagagggaa agcaaaaaaa gtcatctcgt cgggttgtac atcaaacagt ttcgtaggcg
     2221 cctcggaact gcctaatttt tttatgaaca gtcggttgga gtgccagtca actcgaaagt
     2281 gttgattttc agtatctgga tacaaataaa tctgtttgtc ctcgttgctt tctaacaaat
     2341 tcaagcgacg cgcg
//
LOCUS       DAOMXO010000376         2352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26270_length_2352_cov_3.894645, whole genome shotgun sequence.
ACCESSION   DAOMXO010000376 DAOMXO010000000
VERSION     DAOMXO010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26270_length_2352_cov_3.894645"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLH57_08080"
     CDS             complement(<1..411)
                     /locus_tag="PLH57_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRK02609.1"
                     /translation="MKKSSLHFGTQAIHAGQGPDPSTGAIMTPVYLTSTYAQTSPGKH
                     QGYEYSRTHNPTRRALEDCVAALENGSRGFAFASGCAALSTLLMTLKAGDHVVCSDDL
                     YGGSFRIFDKVFKNFGLEFTFVDMTDLNRTESAFK"
     gene            complement(426..1850)
                     /locus_tag="PLH57_08085"
     CDS             complement(426..1850)
                     /locus_tag="PLH57_08085"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HRK02610.1"
                     /translation="MKSPTQTQEFKSQIHQSILQTLGDTPLVRLNKVAEGIDAEIVAK
                     IEFFNPGGSVKDRIGIAMIEDAEKRGVLKPGGTIVECTSGNTGMGLAIAAAVRGYKCI
                     FVMPDKMSAEKIRNLRAFGAKVVITPTAVAPDDPRSYYSVAKRLAQETPNAVHMNQYD
                     NLSNRKAHFESTGPELWGQTNGDFDAFVAGMGTGGTITGTGMFLKQKKNSIQIVGVDP
                     IGSILHDYKKTGKMTNAHTYKIEGIGEDIIPLNYDFQFIDDIVQVNDKEAFQMTRSLL
                     HQEGIFCGVSSGAAVCGAIKYARKLIAEGRKRPRIIVLLPDSGDRYLSKVFDDDWMRE
                     NGFLEGGLGTVRELLDAVHPPSRGALITAKASDAVGTIVTMMRDKGISQVPVLRGKQM
                     EGLVHESSLLSALFAGKIRTSDSIASLVETTFVQVSLDDDVERLSQSTAAGITPVVVD
                     AGEIISIVSKIDLITYLSSRNQKA"
     gene            complement(1819..2331)
                     /locus_tag="PLH57_08090"
     CDS             complement(1819..2331)
                     /locus_tag="PLH57_08090"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF020460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRK02611.1"
                     /translation="MLLEKPFVFIKSNSGTYGMGILKIEDPAELRSLNRRDRNKMHVV
                     KNNLVVHDVIVQEGIPTRFQVDGVFAEPVVYSLGSELLGGFLRKNPTRSKIDNLNSKG
                     MVFQKLCMTDLRREADRDLSLELVFGTMATISVAAVGLEIAKHGSPLRMLPKIDNSMG
                     DEITHANPGI"
BASE COUNT          569 a          567 c          575 g          641 t
ORIGIN      
        1 cttaaacgca ctttcggtcc gatttaagtc ggtcatgtcg acgaaagtaa attctagacc
       61 gaaattttta aaaactttat cgaaaatgcg aaaactaccg ccgtaaagat catcggagca
      121 aacaacatga tcgcctgctt ttagtgtcat caaaagagtg gatagcgccg cacaaccaga
      181 agcgaacgcg aagccgcggc taccgttttc gagtgcagct acgcaatctt cgagggcacg
      241 tctggtggga ttatgtgtcc gagaatattc gtaaccctgg tgtttgccag gcgaagtttg
      301 cgcataggtc gaggtcaaat agactggggt catgatcgcg ccagtgctgg ggtcgggccc
      361 ttggcctgcg tgaattgctt gagtaccaaa gtgcaaagac gattttttca taacgccccc
      421 tgtttctagg ccttttgatt tcttgagctt aggtaagtga tgagatcgat tttggataca
      481 atcgaaatga tctccccggc atcaactact acgggtgtaa ttcccgctgc ggtggactgg
      541 ctgagtcgtt caacgtcgtc atcaagggaa acttgcacaa aagttgtttc gaccagactc
      601 gcgatcgaat ccgaggtgcg aatctttcca gcaaaaaggg ccgaaagaag gctcgattca
      661 tgtaccaagc cttccatttg ttttccgcga agcaccggaa cttgactaat acctttgtca
      721 cgcatcatcg ttacgatcgt tccgaccgcg tcgctggctt tggcggtgat gagtgcgcct
      781 cgagaaggtg gatgtaccgc atccagaagt tcgcgaacag ttccaaggcc gccttcaagg
      841 aaaccgtttt ctcgcatcca gtcgtcgtca aatactttag ataggtaacg gtccccagag
      901 tcgggcaaca aaacgataat gcgcgggcgt ttgcggccct ccgcaatgag cttgcgagcg
      961 tatttaatag caccgcagac cgcagctccc gacgatacgc cgcaaaaaat accttcctgg
     1021 tgtaagagcg atcgtgtcat ttgaaaagct tctttgtcat ttacctgcac gatgtcgtcg
     1081 ataaactgaa aatcgtagtt gagtgggata atgtcctcac caataccctc aattttatat
     1141 gtgtgggcgt tggtcatttt tccagttttt ttataatcgt gtaaaatcga gccgattgga
     1201 tcaactccga ctatttgaat actatttttc ttttgtttca aaaacatgcc ggtaccagtg
     1261 atggttcctc cggtccccat gccggccacg aacgcgtcga aatctccgtt ggtttggccc
     1321 caaagttcag gaccggtgga ttcgaaatga gcttttcgat tcgaaagatt atcgtattga
     1381 ttcatgtgta cagcgttcgg agtttcttgg gctaggcgtt tggcaacact gtagtaggag
     1441 cgaggatcgt ctggagctac ggcggtgggt gtaatgacca cctttgcgcc gaatgctcgt
     1501 aaatttctaa ttttctcagc cgacatttta tcgggcatta caaaaatgca tttatatccg
     1561 cgcactgcgg cggctatggc gagccccatt ccagtatttc cgctggtgca ttctacaatc
     1621 gtgcctccag gctttaatac tccgcgcttc tcagcatctt cgatcatcgc gatcccgatg
     1681 cggtctttta ccgaaccacc agggttgaaa aattcgattt tggcgacaat ttccgcgtct
     1741 attccttcgg cgaccttgtt gaggcgcact aaaggcgtat cgcctaaagt ttgaagaata
     1801 ctttggtgga tttgtgactt aaattcctgg gtttgcgtgg gtgatttcat cgcccataga
     1861 attatcgatc ttgggcagca ttcgcaaggg agaaccgtgt ttggcgattt ccaatccgac
     1921 tgcggccacc gagatcgtgg ccatggttcc aaacaccagc tctagcgaca aatcgcgatc
     1981 ggcctcgcgg cgtaagtcgg tcatgcagag cttttgaaat accattcctt tggagttcaa
     2041 attgtcgatt ttacttcggg tagggttttt gcgcagaaaa ccaccgagta gctcggatcc
     2101 gagtgaataa acgaccggtt ccgcgaatac gccgtcgact tgaaaacgag tcggaattcc
     2161 ttcctgaaca attacgtcgt gtaccaccaa gttatttttt accacgtgca tcttattgcg
     2221 atccctacga tttagagacc gaagttccgc cgggtcttcg atttttaaaa tacccatgcc
     2281 gtaggtgcca gaattgcttt taataaatac gaagggcttt tctaaaagca attctggcac
     2341 ctacggcatg gg
//
LOCUS       DAOMXO010000377         2346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26362_length_2346_cov_5.116979, whole genome shotgun sequence.
ACCESSION   DAOMXO010000377 DAOMXO010000000
VERSION     DAOMXO010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26362_length_2346_cov_5.116979"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="flgA"
                     /locus_tag="PLH57_08095"
     CDS             <1..816
                     /gene="flgA"
                     /locus_tag="PLH57_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRK02612.1"
                     /translation="DSWIQIRCRHSKNQCCLWNLNGELNRTISREDIQKATASIGLTG
                     YVLRVVGSESVAVQQTHRELLVEEISSKIKEKLVKKFSEDPENIKVENIRISNPVYVS
                     YSGDTGWDLDLPPNLSQKINAKIVSTGVGTQPLGWITAEVARTAKAYVATKTVRPGDL
                     IEKENFELKPINLFGSNDLSFPEDSFPQGRRAQRSIIRGTPLTFSAIEKIQDVRMGDA
                     VTLIIKSDNLTVTTKGTAQSSGAQGDTISVQVNKYNRTFRGRLLSGRQVEVWL"
     gene            813..1562
                     /locus_tag="PLH57_08100"
     CDS             813..1562
                     /locus_tag="PLH57_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRK02613.1"
                     /translation="MNTASTTLLLLLIATLSGCGWVRSLRSEVEDEADTSRAPASDFD
                     YRDPANRMLPPPPLNARDSREAPISGTPVDLAGIRAKSGRVTKQNFIDESMRNENSLW
                     NDDGQSNYLFARNKLKAPGDLVTIVIEDQLRSDMVREVKKILPPEYRDQEIRVPGLTK
                     EVAAAAPDDATRAPAATNSGELSHEDLMTAEVLERYQNGNIRIRGVKRIPFKRSVRNI
                     EVVAIVRGNEISEVDQIKSSRFFEHRVELYR"
     gene            1559..>2346
                     /locus_tag="PLH57_08105"
     CDS             1559..>2346
                     /locus_tag="PLH57_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRK02614.1"
                     /translation="MKKLNVIVIVIVFLATPFVHGSRIKDIASVKGVRDNLLIGYGLV
                     IGLKGTGDSGTDITSKSMIRLFEKMGIQTGATDFKSKNVAAVIATAKLPPFARAGTRI
                     DVTVSSIGDAASLEGGTLLVTPLRAGDQTVYAVSQGPVSLGGLTDGGGKVSFPTTARV
                     PNGALVEKEIDGSFTNKKTFVLSINNPDFTTAARLTKVINTELGGKYAVARDSGTVDV
                     IAPFNFEGSGVELMATIENLDVDADSKARVVLNERTGTVVIGENV"
BASE COUNT          701 a          510 c          580 g          555 t
ORIGIN      
        1 gactcatgga ttcaaattcg atgtcgacac tcgaaaaatc aatgttgtct ttggaatttg
       61 aatggagaat taaatcgcac catttcccgt gaagatattc agaaagcgac cgcatcaatc
      121 ggcctaacgg gttatgtact tcgtgttgtt ggttccgaat cggttgcagt tcaacaaacc
      181 catcgtgaac tgctggttga agaaatatct tcgaaaataa aagaaaaact agtcaaaaaa
      241 tttagcgaag atccggaaaa tatcaaagta gaaaacatca gaatttcgaa tccagtttat
      301 gtttcatatt ctggcgatac gggttgggat ctcgatcttc caccgaactt gagtcaaaaa
      361 attaatgcta aaattgtgag taccggagtc ggaacgcagc cgctcggctg gattacagct
      421 gaagtcgcgc gcactgctaa agcgtatgta gctacaaaaa ctgtgcgacc cggcgattta
      481 attgaaaaag aaaatttcga attaaaacca ataaacttat ttggcagtaa cgatttatcg
      541 tttccggaag atagttttcc gcagggtcga cgggcgcaaa gatcaattat tcgaggcacg
      601 cccttaactt tcagtgccat agaaaaaatt caggatgttc gcatgggcga cgcggtaacg
      661 ttgattataa agtccgacaa ccttacggta actacaaagg gaactgctca gagcagcggc
      721 gcccagggcg acaccattag tgtgcaagta aacaaatata atcgtacatt tcgaggccga
      781 ctattatccg gaagacaagt tgaggtgtgg ctgtgaatac tgcgagcacc acgctcttgc
      841 ttctattaat cgccacgctg tctggttgtg gatgggttcg tagcttgcgt tctgaagtgg
      901 aggacgaggc ggacacgagt cgagcaccgg caagtgattt tgactataga gatcccgcga
      961 atcgaatgtt accaccaccg ccattaaatg cgcgcgactc tcgcgaggct ccaatctcag
     1021 gtaccccggt tgacttggca ggaatcagag caaaatccgg ccgcgtgacc aaacagaact
     1081 ttatcgatga atccatgaga aatgaaaaca gcctctggaa cgacgacgga caaagtaatt
     1141 atctatttgc gcgaaacaaa cttaaggccc ctggcgacct tgtcaccata gtcatcgaag
     1201 atcagttaag aagcgacatg gtacgggaag taaaaaaaat actgccgccc gaatataggg
     1261 accaagagat tcgagttccc ggcctaacaa aagaagtggc agcggccgca cccgacgatg
     1321 ctaccagagc accggctgca accaacagtg gcgaactgtc tcatgaggat cttatgactg
     1381 ccgaagttct cgaaaggtat caaaacggaa acatacgtat acgtggagta aagcgcattc
     1441 cctttaaaag gtcagtgcgc aatatcgaag tcgtagcaat cgtacgtggc aacgaaatta
     1501 gtgaagtaga ccagatcaag tcatcgagat tttttgaaca tcgagtggag ctctatcgat
     1561 gaaaaagctt aacgtcatag ttatagtgat cgtctttttg gcaacgccgt tcgtgcatgg
     1621 ttcccggatt aaggacatcg cttcagtaaa gggcgtgcgt gacaacttgc taattggata
     1681 tggcctcgta attggtctca aaggaacggg tgactcgggc accgatataa cctctaaatc
     1741 catgattcga ttattcgaaa aaatgggcat acaaaccggt gcgaccgatt ttaagtcaaa
     1801 aaacgttgcg gcagtaattg ccaccgcgaa gcttccgccg tttgcccgtg ccggcactcg
     1861 aatcgatgtt accgtttcaa gtattggtga tgctgccagc ctcgagggag ggaccttgct
     1921 ggttactcct ttgcgagcag gcgatcagac agtctacgcg gtatcgcagg ggccagtatc
     1981 tcttggtgga ttaactgacg gcggaggcaa agtatcattt cctaccacgg caagagtccc
     2041 aaatggtgcg ttggtggaaa aggaaatcga tggaagtttt acgaacaaaa agacttttgt
     2101 actttcaatt aacaatccag attttacgac agcagccagg ctcaccaagg taatcaacac
     2161 tgagcttggc ggcaaatatg cagtggcacg agattccgga acagtagatg ttatcgcacc
     2221 ctttaacttt gaaggaagtg gcgtggagtt gatggctaca atagaaaatc tcgacgtcga
     2281 tgcagattcg aaagctcgag tcgttctaaa tgaacgcaca ggaactgttg taatcggtga
     2341 aaatgt
//
LOCUS       DAOMXO010000378         2345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26391_length_2345_cov_3.805677, whole genome shotgun sequence.
ACCESSION   DAOMXO010000378 DAOMXO010000000
VERSION     DAOMXO010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26391_length_2345_cov_3.805677"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1100
                     /locus_tag="PLH57_08110"
     CDS             162..1100
                     /locus_tag="PLH57_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02615.1"
                     /translation="MEFTTFNLAHSNAVGFSLVRDIGERSIRILTELEKKLEHRRILL
                     NLDPTLHHDLVFAFQESIIRKTHRRLIRWAENRGLYIAESDSQGDLLFFSTAPIVGSR
                     FKKFSKNEIGRNFGVQEILVELSEPDNPANSRLIRVVQTHTSFSTPGQLKPEHGIHLK
                     EIAEFIHSPPAVLPENTPEVFLGDINMSRNLRYRNGMVDGATPAWGSFVTSLKPRLEW
                     IPNDDPTWDEINNPLARDPAVILRIIGLGSKGKGWGLQNTVLDHIFKSAHWTTQSIQV
                     TFKEPVEVLSAFEPEEKARRFVQLSDHYGCSAILKL"
     gene            1173..>2345
                     /locus_tag="PLH57_08115"
     CDS             1173..>2345
                     /locus_tag="PLH57_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02616.1"
                     /translation="MYFLRLILIASIFVTHSSVALAQKCKDILIVKDQRLAQKLLIEK
                     LQQYVQPNFIGLSVATFPGGAYEVEYESTLREMNVDLIVIENPLANSSEKTQLGPENG
                     YFEKPEKVHWTLPVQPFSEYLTTRSGKERRRILSKLKKSESVHVVLAPLKVSTFDEWY
                     QIYQKEVVGKTRGRRALLPQWALKQGGDLGKKYHGLFFRDPSNGQMLGGALLSLDKRS
                     HTLMISYAAYSSDSKQLELSVRTVEQAMSFAVRSGFLTLSYGYDSNFYGHHLTPGLME
                     YKAGMGFTPNAAGRTEVVKVLNPVKLLDPYAFFTFSDRDGSPASRLNIFSESGISLIP
                     PANSQLQHFPRSFGVPATTDKIPNAHVPFTLLSQLQDRAFFELLRDDILTKKQSHWI"
BASE COUNT          627 a          596 c          495 g          627 t
ORIGIN      
        1 ttagagcctt agctcgtttg cacatctcgt atgaatttgc tacgactctt aattgagtgg
       61 aaaactttat ccttcgctgg ggactccggt taagcgtctt aaccttgggt ctcttattac
      121 atagcttcgt cgtggcagct aacgggaagt tctcgcatac cgtggaattc acgaccttta
      181 atcttgccca cagtaacgct gttggctttt ccctcgttcg cgacatcgga gaacgttcaa
      241 ttcgaattct tacagaactc gaaaagaagc tcgaacaccg cagaatatta ctgaacctcg
      301 accccaccct gcaccacgac ctagttttcg catttcagga atcaatcatt cgtaaaactc
      361 atcgccgcct tattcggtgg gcggaaaacc gcgggctcta cattgctgag agtgattctc
      421 aaggtgactt gctgtttttt tctactgctc caatcgtagg ctcgcgtttt aagaaattta
      481 gcaaaaatga aatcggtcgc aattttggcg ttcaagaaat tctggtcgag ctttcagagc
      541 ccgacaaccc ggcgaattcg cgactaattc gagtggttca gacacacact tcctttagca
      601 ctccgggcca actgaaaccc gagcacggca ttcacctcaa agagatagct gaattcatac
      661 attcgccacc ggcggtcctg cccgaaaata cacctgaagt atttttgggt gatatcaata
      721 tgagccgtaa tcttcggtac cgcaacggta tggtcgatgg agccacaccg gcctggggta
      781 gtttcgtaac ttcactaaaa cccagactgg aatggatccc aaacgacgac ccaacgtggg
      841 atgaaattaa caatcccctt gcccgagatc cggccgttat tttgcgaatc atcggtttgg
      901 gtagcaaagg caaaggctgg ggactgcaaa ataccgtgct tgatcacatt tttaagtccg
      961 ctcattggac aactcagtct attcaagtca cttttaaaga gcctgttgaa gttctttctg
     1021 ctttcgaacc tgaagaaaaa gcccgtcgtt ttgtgcagtt gagtgatcat tacgggtgtt
     1081 ctgccatcct gaaactctaa atagactact cgcgttattt tctttactaa aacaatcgga
     1141 aataatataa gaccttagac ttaaagggtt ttatgtattt cttgcgcctc attctcatag
     1201 cgtctatttt cgtgacccat tcttcggtcg cgcttgccca aaaatgcaaa gacatcttaa
     1261 tcgtcaaaga tcagcggctc gcccaaaaac tgcttatcga aaaacttcaa caatacgtgc
     1321 agccaaactt tattggcctg agcgtggcaa cattccccgg cggggcctac gaagttgaat
     1381 atgagtccac cctacgcgaa atgaacgtgg acttgattgt tatagaaaac ccgctcgcga
     1441 attcatcaga gaaaacgcag ctgggaccag aaaatggcta cttcgaaaaa ccagaaaagg
     1501 tccactggac tctgcccgtg caaccatttt cagaatacct caccacgcgc tccggaaaag
     1561 aacggcgccg tatactatcg aaattaaaaa aaagtgaatc cgttcacgtc gtactcgcgc
     1621 cattgaaagt aagtactttc gatgagtggt accaaatcta ccaaaaagag gtcgtgggca
     1681 aaacacgcgg ccggcgagcc ttattacccc agtgggccct gaaacagggc ggcgatcttg
     1741 gaaaaaaata ccacggactt ttttttcgtg atccttctaa cggacaaatg ttgggcggcg
     1801 cacttctttc gctcgataaa cgcagccaca ctttgatgat ttcatatgcg gcttattcta
     1861 gtgactccaa acaattggaa ctatcggtac gaaccgttga acaagctatg tcatttgccg
     1921 ttcgttcggg ctttctaact ctgagctacg gttatgattc taatttttat ggacatcatt
     1981 tgacacctgg tctcatggaa tacaaagccg gaatgggttt tacgccgaat gcggctggtc
     2041 gcacagaagt tgtaaaggtc ctgaacccag taaagttatt agacccatat gcgtttttca
     2101 cattttctga ccgcgacggc tcacctgcgt cgcgcttaaa tatattctct gagagtggaa
     2161 tcagtctcat tcctccggct aactcccagc ttcaacattt tcccagaagc ttcggcgttc
     2221 ctgcaacgac tgacaaaatt cctaacgccc acgtcccctt tactctttta agccaacttc
     2281 aagatcgcgc tttttttgag cttcttcgcg acgacatcct gaccaaaaaa caatcccatt
     2341 ggatc
//
LOCUS       DAOMXO010000379         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26441_length_2342_cov_4.615654, whole genome shotgun sequence.
ACCESSION   DAOMXO010000379 DAOMXO010000000
VERSION     DAOMXO010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26441_length_2342_cov_4.615654"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PLH57_08120"
     CDS             complement(<1..743)
                     /locus_tag="PLH57_08120"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HRK02617.1"
                     /translation="MENTSPLLFDDSEKKFFIAMGNKAMGPLSAQEVYERIQRGEVGL
                     LHYYWRKGLSDWKRLAEEKEFAVLVPKKPASSSIKTLRSRIGDITKDVELTQTRVNRP
                     YYLYTNNTQYGPFSEDELLRVLKTQKVSRNAYAWTAGWPNWKRLSSIPEFAEFVASTP
                     APKPTSKASSKSKSKTKSSLKKVPASNDQTMGVRMGTVAGEDLSDKRGGPRRPLVARL
                     FLHNNQDVIIAVCRDISIGGMQVLTDRVPG"
     gene            827..1678
                     /locus_tag="PLH57_08125"
     CDS             827..1678
                     /locus_tag="PLH57_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HRK02618.1"
                     /translation="MYAEQWNGAATGADAPVLLFVHGFGEHLARYRGIFERLKGPFAQ
                     GYAFDLRGHGRSQGTRGHIEHFDDHVDDLEAMVSHIIEKTSRPVVLLGHSMGGLITLR
                     YLLRTGLQTRVRAAAVSSPYLKLAFEAPLLKLVAAYALKRALPSLHLRAELNPSHLSH
                     DPHVVEAYVNDRLVHDRLTSRMFFELQGAAADTRHQIRPIGCPLLFLVPGEDRLIDAA
                     ATRKFYQNLKHRAKDWAEFPGMYHEVLNETRHDEVDRVFLTWVARASEVKKRKQGESY
                     HEHADET"
     gene            1656..2213
                     /gene="pyrE"
                     /locus_tag="PLH57_08130"
     CDS             1656..2213
                     /gene="pyrE"
                     /locus_tag="PLH57_08130"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRK02619.1"
                     /translation="MNTQMKLNLLKLIRTLSYREGQFTLASGKTSTFYVDLKNVTLHP
                     DGVRLIAQMAWSLLNPRDFGGVGGPTLGADPLATALSLGAKEQGVVLPAFILRKEPKK
                     HGTSKWIEGRENLKSGARLLVVEDVVTTGGSSLRSIEKIREEGFTVDTCLCVLDRLEG
                     GAQALAAAGVKCVALATIEELLASR"
BASE COUNT          539 a          560 c          631 g          612 t
ORIGIN      
        1 ccaggaacac ggtcggtgag cacttgcatg ccgccaatcg aaatatcgcg gcacacagcg
       61 atgattacat cttgattatt atgtaaaaat aaacgcgcca ccaacggtcg ccgcggaccg
      121 ccgcgtttgt cgcttaagtc ttccccggcc actgtgccca tgcgcacgcc cattgtttgg
      181 tcgtttgaag cgggcacttt ttttaaacta gatttggttt tggacttcga tttcgaacta
      241 gccttgctcg taggttttgg ggcaggcgtc gaagctacga actccgcgaa ttccgggatc
      301 gaactcaaac gcttccaatt aggccatccg gcagtccaag cataggcgtt gcggctaact
      361 ttttgagtct ttaaaacacg cagtaattcg tcctcggaaa aggggccgta ctgcgtgttg
      421 ttagtgtaca aatagtacgg tcgattaact cttgtttgag tgagttcgac atctttagtg
      481 atatctccga ttcgcgaacg aagagtttta atcgaactac tggccggctt tttgggaacc
      541 aagaccgcga attctttttc ttcggccaga cgtttccaat cgctgaggcc cttgcgccaa
      601 tagtagtgca agagcccgac ttcgcccctt tgaattcgtt cgtagacctc ttgggcgcta
      661 agggggccca tggctttgtt gcccatggcg atgaagaatt ttttttcgga atcgtcaaag
      721 aggagcggac ttgtattttc catataagaa attgtagttt gagtccgtgg caacctacaa
      781 ctcttttgtg atggaacgcc cccgtaaggg agcggaacct ttgaagatgt atgcggaaca
      841 gtggaacggc gcagcgaccg gagccgacgc tcctgtgtta cttttcgttc acggattcgg
      901 cgagcatcta gcacgatatc gcgggatttt tgaacgactt aaggggccct ttgcgcaagg
      961 ttatgctttc gatttgcggg gccacggtcg ttcacaaggc actcgtggcc acattgagca
     1021 tttcgatgat cacgtcgatg atctcgaagc tatggtgagt catataatcg aaaaaacgtc
     1081 gcgtcccgtg gtcctcttgg gccacagtat gggtgggctg atcacgttgc gctacctgtt
     1141 gcgtaccggg cttcaaacgc gggtgagagc agctgcggtt tcgagcccgt atttaaagtt
     1201 agcgtttgaa gcccccttgt taaaactcgt ggcggcttat gcgctaaaac gggctttgcc
     1261 gtcactgcat ttgcgggcag aactcaaccc ttctcatctt tcacatgacc ctcatgttgt
     1321 tgaggcttac gtaaacgacc gtctcgtaca tgatcgactc acgtctcgaa tgttttttga
     1381 actccagggg gctgcggccg atacgcgaca tcagatccgt ccgatcgggt gtccgctttt
     1441 atttttagtt cccggagaag accgtctaat tgatgctgcc gcgacccgta aattttatca
     1501 gaacctaaaa caccgcgcca aagactgggc agagtttccc ggcatgtatc acgaagtatt
     1561 gaacgaaact cgtcacgatg aagtggaccg agtgttttta acctgggtgg cacgggcgtc
     1621 cgaagtcaaa aaacgcaagc aaggggaaag ttatcatgaa cacgcagatg aaacttaatc
     1681 ttttgaagct tatccgcacg ctatcctatc gggaagggca gttcacttta gcctccggta
     1741 aaacgagcac gttttatgta gacttaaaaa atgtgactct gcatcccgat ggcgtgagac
     1801 taattgcgca aatggcttgg tccttgttga atccccgaga tttcggtggg gtaggcgggc
     1861 ccactctggg agcggaccct ctggccactg cgcttagtct tggggcaaag gagcaaggcg
     1921 tagttctgcc cgcatttatt ttgcgcaaag agccgaaaaa acacggaact tcgaagtgga
     1981 ttgaggggcg ggagaatctt aaaagcggcg cccgcctgtt ggtggtggag gacgtcgtga
     2041 ccactggtgg gtcgtcttta agaagtatcg aaaaaattcg tgaagagggt tttacggtcg
     2101 acacctgttt gtgcgtgctc gatcgtctag aaggtggagc tcaggctctt gctgcggcgg
     2161 gggtgaaatg tgttgcgctc gccaccattg aagaacttct ggcctcgcgt tagactttga
     2221 gtatgaatat ctcgcagcga ttgataatcg tcctttgttt cagtttattg acggcctgtg
     2281 caagcacaca tccattagat cgattcgtcg atgagcaaaa aactcgtgtc agcgaaaaag
     2341 cg
//
LOCUS       DAOMXO010000380         2334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26577_length_2334_cov_4.207547, whole genome shotgun sequence.
ACCESSION   DAOMXO010000380 DAOMXO010000000
VERSION     DAOMXO010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26577_length_2334_cov_4.207547"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..982)
                     /locus_tag="PLH57_08135"
     CDS             complement(71..982)
                     /locus_tag="PLH57_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRK02620.1"
                     /translation="MKTSPTSRENNIDWNHVFYFSEVAAHGSIKNAAPKLHLSPSTLS
                     THISQLEKDLAIKLFHRRHRKLSLTAEGVKLYQYAKQMFESGCRLIDVVSPVSLGHYP
                     ISIGLVPCPSMPVAYRIVTNYLTKYDPVNIRVSQAKHDELEKGLAEARFDFGFSNRVP
                     DRKDIGHLLVARSALKFFVAKNIANESFSTLCSKLPIVICGAESRGLDLVEQLFEDLA
                     LKPKSIVTSDYPGLVLDLCLKERAIGVFGEDSIQNLNLDILCPLPYPERSSNRFDSLY
                     VLWPKDSENSKAIQFLKAQLPTFNKEF"
     gene            1116..>2334
                     /locus_tag="PLH57_08140"
     CDS             1116..>2334
                     /locus_tag="PLH57_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRK02621.1"
                     /translation="MYHYGLIGNCQIAALVSKAGSIDWLCLPRPDSPPVFGRMLDDQG
                     GHFSVEPVGKFSSKQSYLPNTNILVTHVTTEDGSEYQITDFCPRFEQHGRMFRPCSLF
                     RVVTPKKGNAKIKVSCKPVNGWEKEIVKPVRGNSHLRYDLREESLRLVTNMSLTYLCD
                     ETPFVLSEPLYFGLTWSYGIEEDLPKITEQFLHQTTVYWRTWVQHCSIPSLYQKETIR
                     SALTLKLHCYEDTGAILAALTTSLPEEAGKERNWDYRFCWLRDSAFILSAFHSLGHFE
                     EMEGFLKYLLNIAHQHEHSREKLRPVYSLDQSLPLPEKEHLNWAGFRGSKPVRSNNQA
                     AEHIQNDVYGEMILTLAPIFFDERFQHLRTRDHEELLQNLFKFCVRSVSQPDAGPWEL
                     RNGWQEHSFSNLMC"
BASE COUNT          646 a          492 c          549 g          647 t
ORIGIN      
        1 taaccgacgc ccaggctatg actgcgagta tcggagttag tacacttaca aatcgtaata
       61 aacttcgcat ttaaaactcc ttattaaagg tcggaagttg cgcttttaga aactgaattg
      121 ccttcgagtt ttccgaatcc ttcggccaaa gtacgtacaa gctgtcgaat cgattagacg
      181 atcgttctgg atagggcagg ggacacaaaa tatctaggtt gaggttctga atagagtctt
      241 caccaaatac tccaatggct cgttctttta ggcaaaggtc tagaacgagc cccgggtaat
      301 ccgaggtcac gatggatttt ggtttgagcg ctaagtcttc aaataattgt tcgaccaaat
      361 cgaggccacg cgattccgcg ccacaaatga cgatcggcaa cttcgagcaa agtgtagaaa
      421 acgattcatt ggcaatgttt ttagcgacaa aaaacttaag tgccgatcgc gccaccaaga
      481 ggtggccaat gtctttgcgg tcaggaactc ggttcgaaaa tccaaaatca aagcgagctt
      541 ctgcaagacc tttttcgagt tcgtcgtgtt tggcttgaga aacacgaatg ttcaccggat
      601 cgtatttggt caaataattg gtcacgatac gataggcgac cggcatcgaa gggcagggaa
      661 caagcccaat cgatattgga tagtgtccca gcgaaaccgg agaaacaacg tctatcaagc
      721 ggcaaccgga ctcaaacatc tgcttggcat attggtatag ttttacgccc tcggcggtca
      781 gactaagttt gcgatgtcgt cgatgaaaga gcttaatcgc aaggtctttt tccaattgag
      841 aaatgtgagt gctcaaagtc gaaggactta agtgcaactt aggggcggca tttttgattg
      901 agccatgggc ggcgacttcc gaaaagtaga agacgtggtt ccagtcaatg ttattttctc
      961 gcgaagtggg cgatgttttc attgtaaaag tccgcaattg ctcaaaattt cagtttgatt
     1021 tttttgtgtc gagaactatt tcacattgga gttttcaatg caatgagata acttattgaa
     1081 ggagtgagca ttttaaatgg ggagaatgtt gcagcatgta tcactatggt cttatcggaa
     1141 actgtcagat cgcagctttg gtttccaaag caggctccat tgattggctg tgtcttcctc
     1201 ggcccgatag ccccccagtt tttggacgca tgctcgacga ccaaggcggg catttttctg
     1261 tcgaaccagt tggaaaattt tcttcgaaac aaagttactt gcccaacacc aatattttag
     1321 taacgcatgt tacgacggaa gacggcagcg aataccaaat tacagatttt tgccctcgat
     1381 tcgaacaaca tggtcgaatg tttcggccct gttcgttatt tcgagtggtt acacccaaaa
     1441 agggcaacgc taagatcaag gtatcctgta aacccgtgaa cggatgggaa aaagaaatcg
     1501 taaaaccagt acgaggcaat agtcatcttc gatatgattt gcgcgaagaa agcctgcggc
     1561 tggtaacaaa catgtcgtta acctatcttt gcgatgaaac accgttcgtt cttagtgagc
     1621 cactttattt cgggctaact tggagttacg ggatcgaaga agatcttcca aaaattactg
     1681 agcaatttct tcatcaaacc acggtttact ggcgcacctg ggttcagcat tgttccattc
     1741 ccagtcttta tcagaaagaa acgattcgtt ccgcactcac acttaaactt cactgctacg
     1801 aagacaccgg tgcaatttta gcggcactta cgacaagtct gccggaagaa gcgggaaaag
     1861 agcgtaattg ggactatcgg ttttgttggt tgcgagattc agcttttatt ttgtcggcct
     1921 ttcatagtct cggtcatttt gaagagatgg aaggttttct aaaataccta ttaaacattg
     1981 cacatcagca tgagcattcg cgtgaaaaat tgcgcccggt ttacagtttg gatcagtctt
     2041 tgcctttgcc ggaaaaggaa catttaaatt gggccggatt tcgtggctca aaaccggtaa
     2101 gaagcaataa tcaagcggct gagcatattc aaaatgatgt ttatggagaa atgattctta
     2161 ctctcgcacc gatttttttt gatgagcgat ttcagcactt gcgaacccgc gatcacgagg
     2221 agcttttaca aaatcttttt aaattctgtg ttcgcagtgt ttcgcaaccg gacgccggtc
     2281 cttgggaatt acggaatgga tggcaggaac actccttttc taatctcatg tgtt
//
LOCUS       DAOMXO010000381         2329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26663_length_2329_cov_3.652595, whole genome shotgun sequence.
ACCESSION   DAOMXO010000381 DAOMXO010000000
VERSION     DAOMXO010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26663_length_2329_cov_3.652595"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2140
                     /locus_tag="PLH57_08145"
     CDS             <1..2140
                     /locus_tag="PLH57_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02622.1"
                     /translation="DRVFTQVFNVPLSLKILPESDPLKKLSKRNAVVEWQKAGYAGAF
                     SLKQQVIETILGQMHRDSGVKVQASFLIVGSSRSGKTFLWETLVKMLGLKKYDFYAKD
                     VAENDDAQAIKIGITESLGRKDSTLDARDLTGAGDVMSLETQLKHLEHFLTRKNGSRG
                     FILFDDIHRFPPRERRILTERIRALLEQEYYVAQSTFDKKIVERPSSGLAVFATLNPP
                     DDVTRIREFDPNWHPWSGEKPHPLILAKAALHDKEVPIEESFFTRWKLPLFIDKFPIG
                     AKSPELVNNIVKLSRDALEDSGNPFLVTPEVIASLVYGFRDENAKTFLSDSSSALIQL
                     IARAQSAYSAPKIIALREGSPLPDLSAEVKIEPYAESKNKKPQIRDHVNQHFVALSFD
                     EGYTGHLELLRLMVDSFRIRVYEELVQELSQSERYALDVKIASSVLAPALYGLGRHMM
                     ERPKLPLKFLQLDAYKLGGTSKADELVFHEELKKLADREPTEFFPQGFDRTQRIKGLI
                     SAIEKRRGTGPEGFTRQQVLREYVEYAQIELYEAFLATMRLRTTQPFPKPMDWLRSLT
                     PQSAGEASPFKVLAPKLVQVFDRFRKDILDARLYHGVPSENIPDFYDVSRLFGFVLDK
                     AMTRLPWSESTTFMVDSLSLLSDDMNLAQMRGVQDFFFLRPTAIIRPTTRDLMIESVV
                     SSPVVQGIDEKRRDELEKRFQLTCKEILEK"
BASE COUNT          637 a          525 c          563 g          604 t
ORIGIN      
        1 ggaccgagtt ttcacgcaag tgttcaacgt accacttagt ttaaaaattc tccctgagag
       61 cgatccgctt aaaaaacttt ccaaacgcaa tgcggtcgtt gagtggcaga aggcgggcta
      121 cgcaggggca ttttcactca aacaacaagt aattgagacc attctcggcc aaatgcatcg
      181 agactcgggc gtaaaagttc aagcatcctt cctaatcgta ggtagctcgc gttcagggaa
      241 aacattcctt tgggaaacat tggtcaaaat gttgggacta aagaagtacg atttctacgc
      301 aaaagatgtt gctgaaaacg acgatgctca agcaataaaa attggtataa cggaaagtct
      361 tgggcgcaaa gattcgacac tcgacgcccg tgatcttacc ggtgccggcg acgtgatgag
      421 tctcgagact cagctcaagc accttgaaca tttcttaact cggaaaaacg gaagtcgtgg
      481 gttcattctt tttgatgata ttcaccgatt cccgccgcga gagcgtcgca tattaaccga
      541 acgaattcgc gctctactag agcaagagta ttatgtggcg cagtccacat ttgataaaaa
      601 gatcgtcgag cgcccaagta gtggattggc ggtttttgcc acgttgaacc cgccggatga
      661 cgttacccgc atacgggagt ttgatccaaa ttggcatccg tggtcgggcg aaaaaccgca
      721 cccgcttatt ttggctaagg ctgccttaca tgacaaagaa gtaccgatcg aagaaagctt
      781 tttcacgcga tggaagctac ccttgttcat tgataaattt ccaatcggcg ccaagagtcc
      841 ggaactcgta aataacatag ttaaactttc gcgcgatgct cttgaggatt ccggaaatcc
      901 gtttttagtc acacccgaag ttatcgcctc tctggtctat ggatttcgcg acgaaaacgc
      961 taaaacgttt ctttcagatt cttcttccgc gttaattcag ttaatcgctc gtgctcagtc
     1021 ggcctattcc gcgcctaaaa ttatcgctct acgtgaaggc agccctttgc ccgatctatc
     1081 tgcagaagta aaaatcgaac cctatgcgga gagcaaaaac aaaaaaccgc agatacgcga
     1141 tcatgtaaat cagcactttg tagcgttgtc atttgacgaa ggttatactg ggcacctgga
     1201 attgctacgt ctcatggtag actcgtttcg aatccgagtt tacgaagagc ttgttcagga
     1261 actgtcgcaa tcggaacgct acgcgcttga tgtaaaaatt gcatcttcag ttttggcgcc
     1321 tgccctctat ggacttgggc gacacatgat ggagcggccg aagttgccac taaagtttct
     1381 gcagctcgat gcttacaagt tgggtggaac ctcaaaagct gacgaattag ttttccacga
     1441 ggaattaaaa aaactagcag accgtgaacc gactgaattt tttccgcagg ggtttgatcg
     1501 gactcagcga attaagggtt taattagcgc aattgaaaag cgacgtggca cgggtcccga
     1561 gggattcacg cgacaacagg ttttaagaga gtacgtcgag tatgcccaga tcgaacttta
     1621 tgaagctttt ttagcaacga tgcgattaag aaccacccaa ccatttccta aaccaatgga
     1681 ttggttgagg tcgctcacgc cgcagtctgc cggcgaagcc agcccgttta aggtgttagc
     1741 tccaaaattg gtccaagtgt ttgatcgttt cagaaaagat attctcgacg cacgacttta
     1801 tcacggcgtg ccctcagaaa atattccgga tttttacgat gtatcgagac tgtttggatt
     1861 tgttttggat aaggcgatga cacgcctacc gtggtcggaa agcacgacct tcatggtcga
     1921 ttcgttaagt cttttgagtg acgacatgaa tttagcgcaa atgcggggtg tgcaggattt
     1981 cttctttcta cgccctaccg cgatcattcg acctactacg cgtgacttaa tgattgagtc
     2041 agtggtgagc tcgccggtag ttcaagggat cgacgaaaag cgccgggacg aattggagaa
     2101 gcgattccaa ctaacctgta aggaaatttt ggaaaagtga gtgaggcgta tttaatgcga
     2161 ttacctagaa tttttcgaaa aactttattg atttcggtga cggtaattgg ctttgcaaac
     2221 agtaacgccg ctgaacctcc aaaaaaacca ggaatcgttg ctgatgacgt acctggtgca
     2281 gcggtttgtc gcgatataat ttctgcagcg gttccacaaa aagaaattt
//
LOCUS       DAOMXO010000382         2322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26784_length_2322_cov_5.313189, whole genome shotgun sequence.
ACCESSION   DAOMXO010000382 DAOMXO010000000
VERSION     DAOMXO010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26784_length_2322_cov_5.313189"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLH57_08150"
     CDS             complement(<1..216)
                     /locus_tag="PLH57_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02623.1"
                     /translation="MRNFVLNIALFLFSVVVFFGSEAHAQPTNIQEIRFIGISKIMAR
                     DLEGELPIVPGDFWKENLHGVIRSTLKK"
     gene            complement(230..>2322)
                     /locus_tag="PLH57_08155"
     CDS             complement(230..>2322)
                     /locus_tag="PLH57_08155"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRK02624.1"
                     /translation="GGRVYGGFRRGSYVAENIDIHKRYGRILGDIVFGSDDILKMKLR
                     TENASTLDVDRLAALGIPYSAPLSIKIDVGGKWGALNGVVESQIGSGVIKGYPIGASR
                     FRLDLGGARWKGNLAVFDTQFENTFDLGWTPGSESHVEFRANSFRFHPLLIGLNTGLA
                     SDSALYGNLGGSAKFSFKTGQVSRLTGDFALDSFVLRRTGYELALGAPVRFSLRNGNY
                     DFTGAEIRSTALGGTQKLNIDGRVREGIINYKLDGGLHLRLLEFVTSEIAGGEGAAAI
                     QARLSGAADAPNLDANLAVKKGYLRLSAVEQPFEEIEAALTVKDNRLAIQQITSRFAG
                     GVVRAQGTALVHLTSVPEFEMSAEIENARVKVYPVTFARSTGKLHLKGSSLPYAVTGN
                     IKVAEAMIRENFNLSEGSRILRSSKFMPSTTSGNREYSLFDLNINVVADRGILVKNEL
                     FDAELRGNLRVINSVKTPRLLGTVDVLSGKLLFKDSYFAIDNGNLRFTNPTVIDPEFD
                     FSGFTEQKGYKVHLIAGGRVSDYKFNLTSQPPLSQNDIVNLLTLGVTSSEYQSLSREN
                     RDAYSRDEVYGLLFNQSSINRGIKEKFGLNVRLDQAQVTTPDNVFRAKSADASENLAP
                     KVVLQKQVTKRLSASVGTTVGVGDSQERSANLEYDIHRNWSVLGTYEDQRGAQLKQSR
                     TSLGADLKFKMRFK"
BASE COUNT          621 a          617 c          485 g          599 t
ORIGIN      
        1 tttttttaag gtacttcgaa ttacgccgtg taaattttct ttccagaaat ctccgggaac
       61 aatcggaagt tcgccctcga gatctcgcgc cataatttta gaaataccaa taaaacgaat
      121 ttcttgaata ttggtaggtt gcgcgtgcgc ttcagatccg aagaacacaa ccactgagaa
      181 caggaaaagc gcgatgttga ggacgaaatt tcgcactaaa gactctccac tatttaaatc
      241 gcatcttaaa cttcaaatca gctcccaaag atgtacgaga ctgtttcaat tgcgcgccac
      301 gttgatcttc gtaagttcca agaaccgacc agtttcggtg aatgtcgtac tccaagtttg
      361 cgctccgctc ctggctatca cccacaccta ctgtagtacc gacagacgcg ctcagccgct
      421 tcgttacttg cttctgcagc accactttcg gcgcaagatt ctctgaagca tcggccgatt
      481 tggcacgaaa aacgttatct ggggtcgtta cttgcgcttg gtccaaacga acgtttaaac
      541 caaacttttc cttaataccg cgattaatac tcgattgatt aaacaacaag ccatacactt
      601 cgtctcgcga ataagcatcg cgattttcac gcgacaaact ctgatactct gaggacgtta
      661 caccgagtgt taacaggttg acgatgtcat tttgagataa aggcggctgg gaagttaaat
      721 taaacttgta gtccgacact cgcccaccag cgatcaggtg aactttatag cctttttgtt
      781 cagtgaaccc gctaaaatca aactccgggt ctataacggt agggttagta aatctcaaat
      841 taccgttgtc gatggcaaaa tagctgtctt tgaacagtag ttttcctgat aaaacgtcta
      901 cagttcccaa taaccgcggt gtctttacgc tgttaattac ccgtagattg ccgcgcagct
      961 cggcgtcgaa cagttcgttt ttaactaaaa tgcctcgatc tgcgacaaca ttaatattta
     1021 agtcaaacag actatattct cgattgcccg acgttgtact cggcataaat ttggaggacc
     1081 gcaaaatacg cgaaccctcg gacaagttaa aattttcacg aatcatggct tctgcgactt
     1141 taatgtttcc ggtcacagcg taggggaggg aactgccctt gaggtgaagc tttccggtac
     1201 tcctggcaaa agtaactggg tacactttca ctcgagcatt ttcgatttcg gcgctcatct
     1261 caaactctgg cacacttgtg aggtgcacca gtgcggtgcc ttgagcccga acgactccac
     1321 ccgcgaacct cgaagttatt tgctgaattg ccaagcgatt atctttaacc gtgagagccg
     1381 cctcgatttc ttcaaagggc tgctctacgg cactcaaacg taaataacct ttttttactg
     1441 cgagattggc atcgagattc ggcgcatctg cggctccact aagccgcgcc tgaatcgccg
     1501 cggccccttc accacctgcg atttcggagg tcacaaattc gagtaatcgc aaatgtagtc
     1561 caccgtctag tttgtaatta ataattcctt ctcgtacacg cccgtcgatg ttcagttttt
     1621 gggtgccacc aagcgcagtc gagcgtattt ccgcaccggt gaaatcgtag ttcccgtttc
     1681 tcaacgaaaa cctaaccggc gcgcccaatg ccaactcgta tccagtccga cgcaatacaa
     1741 aggaatccag cgcaaaatcc cccgttaagc gactaacctg acctgtttta aagctaaatt
     1801 tcgcacttcc gcccaagttt ccataaagcg cggaatccga ggccagacca gtgtttaggc
     1861 caatcaacaa cggatgaaat cgaaaactat tggctcgaaa ttccacgtgc gactcggaac
     1921 cgggagtcca gcccagatca aacgtatttt cgaactgggt atcaaacact gccaagttgc
     1981 ccttccagcg ggcaccacca aggtcgagac gaaatcgcga cgcccctatg ggatatcctt
     2041 tgatcacgcc gctgccgatt tgcgattcga ccaccccatt taaggcaccc cattttccac
     2101 ctacatctat ttttatcgaa agaggggcag aatatggaat tccgagagcc gccaaacggt
     2161 ctacatctag agttgaagca ttttccgttc tcaacttcat tttcagaata tcatcagaac
     2221 caaaaacaat gtcgcctaat atacggccgt atcgcttgtg aatatcgata ttctcagcaa
     2281 cgtatgatcc gcggcgaaaa cctccgtata cgcggccacc tc
//
LOCUS       DAOMXO010000383         2321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26808_length_2321_cov_4.485437, whole genome shotgun sequence.
ACCESSION   DAOMXO010000383 DAOMXO010000000
VERSION     DAOMXO010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26808_length_2321_cov_4.485437"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..>2321
                     /gene="gyrB"
                     /locus_tag="PLH57_08160"
     CDS             143..>2321
                     /gene="gyrB"
                     /locus_tag="PLH57_08160"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRK02625.1"
                     /translation="MTMNPQANDVSNYNADKIKVLEGLEAVRKRPGMYIGDTGARGLH
                     HMVYEVVDNAVDESLAGYCKNVKVTVHADNSVTVEDDGRGIPVDMHSSGVSAAEVVMT
                     KLHAGGKFNEDGGAYKVSGGLHGVGVSVVNALSDSLKLEIHRSGKVYQQLYKRGNPVE
                     SLKQVGVSEKRGTMVTFKPDAEIFEVLEYNHDTLATRLRELAFLNRGLNIVFTDERNE
                     RTQTFCYEGGIVSFVEFMNKSKEKIHDKVIYFSQEKNMITVELAMQWNDGYSENISSY
                     CNNINTIEGGTHVSGLKTALTRVMNKYAEANGFLKNLKEAVQGEDIREGLAAVISVKI
                     PEPQFEGQTKTKLGNSEVEGFVASILNEKLTDYFELNPGVAKKIIQKAIDAATARIAA
                     RKARDLTRRKTALDFGGLPGKMADCQEKDPALCEIFLVEGDSAGGSAKQGRDRRNQAI
                     LPLKGKILNVEKARFDKMLSFEEIKILITALGAGIGKDDFDITKIRYHKVVIMTDADV
                     DGSHIRTLLLTFFYRQMPEVIEKGYLYIAQPPLYKTKKGNKERYLKNENELNDHLLST
                     GVEGLVVKSANGNVPHSQLLSGVKNALKFKRSVEKLAKKYEVNVMRALLSQNVSVDNL
                     SDSEKLKKIMDRVAVYAKAQSTEIGDIKCDIIKDEAHNCFAGHIEVIKNSTRYFVKVT
                     HDLLTSPEYEDLVKQSLAMNVLGQAPFVVTTENQTTETVSTVEA"
BASE COUNT          745 a          450 c          549 g          577 t
ORIGIN      
        1 ttttcgtcgg aacttgattc gaaacggcga gattatttgc tgaaatattt aaacagcaga
       61 gaatctcaag tttttttgag cacgacagaa atcttgaatc tcgggaaaac attttttgtg
      121 aacagaggaa ggatcgtttc ggatgaccat gaacccgcaa gcgaacgacg tcagcaatta
      181 taacgcagat aaaattaaag tactcgaagg tctggaggcc gtaagaaaaa ggcccggaat
      241 gtacattggc gataccggcg cacgtggtct tcaccacatg gtgtacgagg tcgtcgacaa
      301 cgcagtcgat gaatctctcg ctggatattg caaaaacgtt aaagttacgg ttcatgccga
      361 caattctgta accgtagaag acgacggacg aggcattccc gtcgacatgc atagctcggg
      421 agtttctgca gctgaagtcg taatgaccaa gttgcatgct ggtggtaaat tcaacgaaga
      481 tggtggcgca tacaaggtat cgggcggttt gcacggtgtt ggcgtttcag tagtaaatgc
      541 gttgagcgat agtttaaaac tcgaaattca ccgcagtggg aaagtttatc agcagctata
      601 taagcgcggt aatccggttg aatcactgaa acaagttgga gtgagcgaaa aacgtgggac
      661 tatggtcacg tttaagccgg acgccgaaat attcgaagta ctcgaataca atcacgatac
      721 gttagccacc cgccttcgcg agcttgcgtt tttaaatcgt ggtttaaata ttgtttttac
      781 tgatgaacga aacgaacgaa cacagacctt ttgttatgag ggcggaattg tatcgttcgt
      841 cgaattcatg aataagagta aagaaaaaat tcatgataag gtgatttatt tttcccaaga
      901 gaaaaacatg attaccgtcg aacttgccat gcaatggaac gacggatatt cggaaaatat
      961 ttcgtcctat tgcaataaca tcaatacaat cgaaggtggg acccacgtat cgggcctcaa
     1021 aactgcgctc acccgcgtaa tgaataagta cgccgaagca aacggatttt tgaaaaatct
     1081 aaaagaagca gtacagggtg aagatattcg agaaggtctc gctgcagtaa ttagcgttaa
     1141 aattccggaa cctcaatttg aggggcaaac caaaacgaag ttgggtaata gcgaggtcga
     1201 aggctttgta gcctctattc tgaacgaaaa attaaccgac tactttgagc ttaatccagg
     1261 agttgcaaag aaaataattc agaaagcaat cgatgcagcc accgcacgga tagctgctcg
     1321 aaaagcgcgg gatcttacgc gaagaaaaac agcgctggat tttggcggcc ttccaggaaa
     1381 aatggcggat tgccaagaaa aagatcctgc actttgcgaa atttttctgg tcgagggcga
     1441 tagcgccggc ggatccgcaa aacaaggtcg cgatcgaagg aatcaagcaa tcttgcccct
     1501 aaaaggtaaa attttaaacg tcgaaaaagc tcgcttcgat aaaatgttgt cgtttgaaga
     1561 aattaaaatt ttgatcactg cattgggagc gggaatcggc aaagacgatt tcgatattac
     1621 taagattcga taccataaag tcgtaattat gactgacgcc gacgtcgacg gaagccatat
     1681 tcgtactttg cttctcacct tcttttatcg acagatgcca gaagtaatcg aaaaaggtta
     1741 tctctatatt gctcagccac ctttgtataa aacgaaaaaa ggtaacaagg aacgatacct
     1801 caagaatgaa aatgaactta atgatcatct tctttctacc ggtgttgaag ggttggtagt
     1861 aaaaagtgca aatggaaatg tgccgcattc ccaactgtta tctggtgtaa agaatgcctt
     1921 aaaatttaag cgttcggttg agaaattagc gaagaagtac gaagtgaacg taatgcgtgc
     1981 acttctttct cagaacgtat ctgtggataa cttgagtgat tccgaaaaac ttaagaaaat
     2041 tatggatcgg gttgcagttt acgcaaaagc ccagtccacc gaaatcggag acatcaagtg
     2101 cgacattatt aaagacgaag cgcataactg ttttgcgggc cacatagagg taataaaaaa
     2161 cagtactcgc tatttcgtaa aggtcactca cgatcttttg acgtctcctg aatacgaaga
     2221 tttggtaaaa caatctttgg ctatgaacgt tttagggcag gcgccatttg tagtcacaac
     2281 tgaaaatcaa acaaccgaaa cggtttcgac cgtggaagca c
//
LOCUS       DAOMXO010000384         2309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_26991_length_2309_cov_6.805679, whole genome shotgun sequence.
ACCESSION   DAOMXO010000384 DAOMXO010000000
VERSION     DAOMXO010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26991_length_2309_cov_6.805679"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..323)
                     /locus_tag="PLH57_08165"
     CDS             complement(168..323)
                     /locus_tag="PLH57_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02626.1"
                     /translation="MNHRPEATLAEITHAATFGFGMLSATIIQLARLEGMIDDENRIL
                     KHSPPKS"
     gene            455..1846
                     /locus_tag="PLH57_08170"
     CDS             455..1846
                     /locus_tag="PLH57_08170"
                     /inference="COORDINATES: protein motif:HMM:NF033539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HRK02627.1"
                     /translation="MDYPSKWEKNPWKPKTIIYEPTDEKLTASAGLGPLIDAFTQSPE
                     FVEFRKCLPERVGNSSYSAEHLALIVLSGFWYGHDCLDDLEEFKDDPSVEDKLGGLAT
                     SKTIGNYLRDFEDEHVEKLRKFLIKQGYHYRTRIEGKDVSITFDQDSTFHEQHGVKME
                     GVVMTRYNAKGLDSLHTFDDRGFSYDMELRPGSTFSAKHADHMILRVMDDIPNRYEVQ
                     HYYRGDSAFCNEDCLRAAQSKGLKGTVTAHGNTGWESNIADVTNWQPWRYTEDEIAKA
                     ASQKKSLPLIDVGFMMYEPGWAPGVQYPIVIKRTPTTSDGGQISLLGNGGGFKYWGVL
                     SFRGLYPQTPQEILEFHQKRGNMENFIREGKINFDLKHFPCQSIRANHVYGLLALIAH
                     NFLRAMAILMKPDKPHFAKKLRRKMIHVPGRLVKGAGYFRMKIPKRFYEEVMRCRERW
                     LETFKPARVLSSA"
     gene            1934..2074
                     /locus_tag="PLH57_08175"
     CDS             1934..2074
                     /locus_tag="PLH57_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02628.1"
                     /translation="MTLKSDLHHRIWERAVEAALWPMLSDAPLISLKEHPRGPDGEHP
                     AT"
BASE COUNT          602 a          546 c          606 g          555 t
ORIGIN      
        1 catgaaatct ggcacttagc ggttctactg gggtacgtga gtcactcggc catggtgctg
       61 agtttttata agtagcccta caaaatgtgg gcggcaatgt ctgttggtga cgacgttgat
      121 ttcatccgaa ctcgagacag taggcgcaac ctgactcgga ataagaatta tgacttcgga
      181 ggtgagtgtt tcaagatgcg attttcatcg tcaatcattc cttccaaacg cgccagctga
      241 ataatcgtgg ccgacaacat tccaaatccg aaagtagcgg cgtgagtgat ttccgcgaga
      301 gtcgcctctg gacggtggtt cacgcaattc gacgccagaa tgacgcttca ccgataaagt
      361 gaagcgtcaa tcgtcttccg attccggact ctcgacgatc gaaattccgt gatgtagtct
      421 ccttccgtaa actctctcag cggagatcga catcatggac tatccaagca aatgggaaaa
      481 gaatccctgg aaaccaaaaa cgatcatcta tgagccgacg gacgaaaagc tcacggcgag
      541 tgcgggactt ggccccctca ttgacgcgtt cacgcagtcg ccggagttcg ttgaatttag
      601 aaagtgtctt ccagaacgag ttggcaattc gagctacagc gcggagcacc tggctctgat
      661 cgttctttcc ggcttctggt acggtcatga ttgcctcgat gacttggaag aattcaaaga
      721 tgacccgtcg gtcgaagata aactcggggg actagcgacg agtaaaacga tcggcaacta
      781 cttgcgcgat tttgaagatg agcatgttga aaagcttcgc aagtttttga tcaagcaagg
      841 ctaccactac cgaactcgaa tcgaaggtaa ggacgtttcg attacattcg atcaagattc
      901 cacgtttcat gagcagcacg gtgtgaaaat ggaaggtgtt gtaatgaccc gttacaacgc
      961 caaggggctc gatagccttc acacatttga tgaccgcgga ttttcttacg acatggagct
     1021 cagacccgga tcaacattta gcgcgaagca tgcggatcat atgattttgc gggtgatgga
     1081 cgacattccg aaccgctatg aggttcagca ttattaccga ggtgactctg cgttttgcaa
     1141 tgaggactgt cttcgtgcgg cacagtcgaa gggtctcaaa ggcacggtca ctgctcatgg
     1201 caacaccggc tgggaatcaa atatcgccga tgtcacaaac tggcagccgt ggcgctacac
     1261 ggaagatgaa attgcgaagg ccgctagcca aaagaagtcg ctcccattga tcgatgtggg
     1321 tttcatgatg tacgagcccg gttgggcgcc aggggtgcaa tatccgatcg tgattaaacg
     1381 aacgccgaca acttcagatg gcgggcagat ttcgctcctg ggtaatggcg gcggttttaa
     1441 gtactggggc gtattgtcgt tccgagggct ttatccacaa acgccgcaag agattctgga
     1501 attccatcaa aagcgcggca acatggaaaa cttcattcgc gaaggaaaga tcaatttcga
     1561 tctgaaacac tttccgtgtc agagcatcag agccaatcac gtttacggat tgctcgcgtt
     1621 gattgcgcac aacttcttgc gggccatggc gatcttgatg aagcccgata aaccgcactt
     1681 tgcgaaaaaa cttagacgta aaatgattca cgtgcctggc cgcctggtga agggtgccgg
     1741 gtacttcaga atgaagatcc caaaacgatt ctacgaggag gtgatgagat gtcgtgagag
     1801 gtggctggaa acctttaaac cagcccgcgt tttatcttcg gcgtgacgag gatatgaatc
     1861 gagctctttg acaacttcaa tcagtacgga tcttgcgatt cggaaaactt ggaggagtga
     1921 aggacttgaa cccatgacct taaaatctga cttacaccac cgcatttggg aacgagcggt
     1981 cgaagcggcg ctttggccaa tgctcagtga tgcgcccttg atttcattga aagaacatcc
     2041 gcgcgggcct gacggcgagc accccgctac gtaacttcac tcgtcttgga ggttttcgaa
     2101 gcacaattga gtgccgaagc atcgcgtaag atcgaccgcg aacaaaattg acgatcaaaa
     2161 attcgacttc aaattcttaa cccaaatgac gcgcatctat tttcggatcc gggtagttgt
     2221 gttagcggct ctgactgtgt cttgtccctc tttcggattt gggaattact tgatttcgct
     2281 accaaaggat cgacagttct aagactaag
//
LOCUS       DAOMXO010000385         2303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27111_length_2303_cov_4.875000, whole genome shotgun sequence.
ACCESSION   DAOMXO010000385 DAOMXO010000000
VERSION     DAOMXO010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27111_length_2303_cov_4.875000"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /gene="lnt"
                     /locus_tag="PLH57_08180"
     CDS             <1..1059
                     /gene="lnt"
                     /locus_tag="PLH57_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRK02629.1"
                     /translation="MRVAAITAGALILLFLGFGIWGNHRITQLQALERSFSREFRFSV
                     IQANIGDAEKLESESGFARAIYRVLDTYREMTIDAVKKRPDTDLLIWAETAFPLLYLH
                     FDNPIADQSSIGGRSYIKNVAREAGKPLYFGGYAARGKRDYNSAFLLNSDGKILDYYH
                     KSILLAFGEYVPLGPFSSYIQDLVPAIADFGRGAGPMIMSLPLSERDHVRLAPQICYE
                     GLVPEFSRRSVRDGADVLINITNDSWFGDTSEPHQHLGLTRFRSIELRRPMIRATNTG
                     ISAVLGLSGDLNFQTPLFQPTVMQGTIRLPGPEQKFPSTFYARLGEVFAHFASVVALV
                     FLLRNIKTIGRRLRLGRR"
     gene            complement(1025..>2303)
                     /locus_tag="PLH57_08185"
     CDS             complement(1025..>2303)
                     /locus_tag="PLH57_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02630.1"
                     /translation="RFVCASEKANIPKTSRVVFPTESEALKHGIRIEIPCNFNTSQIR
                     IALLDARALIEGSLMNLHKTVTKVAGLKNTCGGNPNQPGCKEWNRKITEVFTADAQQP
                     LGEPAKFRCLAVDAGLEFEVAPRKTASTLPDPFKTFGDLDASVALTDEEYAGSDYEDW
                     IQEYLETCDDNYSLPKIEKCINGSEAALRNIGPFIKAEDCSNVFWPDGTPVLCRWLEH
                     AEATRARAFAHGVRLKQQSLVEKRDAAEKAFLWEHWDSRKWDSEAYSAYLAAEDNLDR
                     VRDLEYDELLNFQAQIKEYMASHLYEFTVQPTSEWSYRGYSIKDAQRSTVQEVCHWKI
                     DCGAALELFERRFAWRTPYPLKEGCWSGIVNFGNAWHPKEPTIGTLAEGQIHCDRAFR
                     PPAEPLPNPASYDNSGDDSDDDSSDDPTSDDDQ"
BASE COUNT          517 a          542 c          599 g          645 t
ORIGIN      
        1 atgcgggtcg cagctataac tgccggtgcc ttgatcctgc tttttcttgg atttggcatt
       61 tggggaaacc accgcattac ccagttacaa gccttagagc ggagtttcag tcgggagttt
      121 agattttcag tcattcaagc caacattgga gatgccgaaa aacttgagag cgagagcggt
      181 tttgcgcgag cgatttaccg cgttctcgac acatatcgtg agatgacgat cgatgcggta
      241 aaaaaacgcc ctgacacaga tttacttatt tgggcggaaa ctgcttttcc gcttttgtat
      301 ctgcatttcg ataatccgat tgccgaccag tcttcaatcg gtgggcgaag ttatatcaaa
      361 aacgtagcgc gtgaagctgg aaaacctttg tattttgggg gttacgccgc ccgcggaaag
      421 cgggattata attcggcgtt tcttttgaac tccgacggaa aaatattgga ctactatcac
      481 aaatcgattc ttctcgcatt tggagagtac gttccgttag ggccatttag ttcgtatatc
      541 caggacttag tgcccgccat cgccgacttc ggccgcggcg cgggccccat gatcatgagc
      601 cttcctttaa gtgaacgaga ccatgtacgc ctagccccgc agatttgtta cgaagggttg
      661 gtgccggagt tttctcgcag gagtgtgcgc gacggtgcag atgtgttgat aaatattacc
      721 aacgattcgt ggttcggcga cacctctgaa ccgcatcaac atttggggct cacgcgattt
      781 cgcagtattg agcttcgaag accaatgatt cgtgcgacca ataccggaat cagtgcggtg
      841 ctggggctta gtggtgattt aaattttcaa acaccattgt ttcaacccac tgtgatgcag
      901 ggaactattc gtttacctgg acccgagcaa aagttcccgt cgacctttta cgcgcggcta
      961 ggcgaagtat tcgcgcattt tgccagcgtc gtggcgctag tatttttgtt gaggaatatc
     1021 aaaactattg gtcgtcgtct gaggttgggt cgtcgctaga gtcgtcgtca gagtcgtcgc
     1081 cagagttgtc gtacgaagca gggttgggca atggttcggc tggtgggcga aacgctcggt
     1141 cgcagtgaat ttgtccctcg gccagagtac caatggttgg ttctttcgga tgccatgcgt
     1201 tgccaaagtt tacgatgcca ctccaacatc cttcttttaa agggtagggg gtgcgccatg
     1261 caaaacgtcg ttcaaatagt tccagcgccg ccccgcagtc gattttccaa tggcacacct
     1321 cttgtacggt gcttctttgc gcatctttta tcgagtaccc gcgatagctc cattccgagg
     1381 ttggttgaac tgtaaattcg tagagatggc tagccatgta ttcctttatc tgagcctgga
     1441 agttcaataa ttcatcgtat tctaaatcac gaactcgatc tagattgtct tctgccgcca
     1501 ggtaggcgga ataagcttca gagtcccatt tgcgggaatc ccagtgctcc caaagaaatg
     1561 ctttttctgc tgcatctcgt ttttcaacaa gagactgttg ttttaatcga acgccatggg
     1621 cgaaggctcg cgctcgggta gcttccgcgt gctccaacca gcggcaaagc accggcgtgc
     1681 cgtcgggcca gaacacattt gagcagtcct ctgctttgat gaaaggtccg atattccgaa
     1741 gtgcggcttc agagccgtta atacattttt caattttcgg aagactgtaa ttgtcgtcac
     1801 aagtctcaag gtattcttgg atccagtctt cgtagtcact gcctgcgtat tcttcgtcgg
     1861 tcagagcaac tgatgcatct aaatcgccaa aagttttaaa tggatctggc aaggttgaag
     1921 cggttttgcg tggagctact tcgaattcca aacctgcatc aaccgctaaa catcggaact
     1981 tggcaggttc gcccaaaggt tgttgagcgt ccgcagtgaa gacttcagta attttccgat
     2041 tccattcttt gcagccaggc tggttggggt tgccaccaca ggtgtttttt aagcctgcga
     2101 ctttggttac ggttttgtgt aaattcatca gactgccctc gattagggcg cgggcgtcaa
     2161 gtagtgcgat gcgaatttga ctcgtgttaa agttgcaggg gatttcaatg cgaattccat
     2221 gttttaaagc ttcggattcg gtgggaaaca ccacccggga tgttttcgga atgttcgcct
     2281 tttccgaggc gcaaacaaag cgt
//
LOCUS       DAOMXO010000386         2303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27116_length_2303_cov_3.625000, whole genome shotgun sequence.
ACCESSION   DAOMXO010000386 DAOMXO010000000
VERSION     DAOMXO010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27116_length_2303_cov_3.625000"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLH57_08190"
     CDS             <1..291
                     /locus_tag="PLH57_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02631.1"
                     /translation="KELESKLEILRKDSETLIASRENKILDLKRKVDLLEFNYDVLQD
                     KNAIERGNVQKANEKIERVLKVLRLALGMIESDDLTGETGASEQKDKDVQVS"
     gene            310..1689
                     /locus_tag="PLH57_08195"
     CDS             310..1689
                     /locus_tag="PLH57_08195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRK02632.1"
                     /translation="MLNNKLNILIVESDTRQTELMVDLINDVAFRPSVDTTNSPERSL
                     ELIGRSNYQLVIMDTGTGKVDGVSILERIKSISPSTSVIMTSSFATIEEAVKAVRLGA
                     DEYFKKPFNPEQFKLAVRRALDRRDLYSGDEAVVGLMNLLNCCQLISGCLEEEKIFDT
                     VLGYLKRETHSQAAACFIYTNNKKKSEPKRIELSNEQDADVVEVMVSSVNVIQSHATE
                     PGSMKILPKAQGNPEIVIFRFAWNEERSYYVVCASPTWAISVDEVDSRVRLLQSQIHM
                     TARNIDSYRDVQHLLNLDEPTGLFNTRYLHKSLEDLFAKRKAGEKSTFSVLFVDVDKF
                     KSVNDQYGHLIGTKLLHEIGQITRGCLRMGDLAFRYGGDEFVVILKNCPTKAAIDIAE
                     RLRAGVEKNEFLSKEKLNVRLTVSIGVANCPEHATSKRAIIEAADAAMYAVKRSTRNR
                     VYVAELKAA"
     gene            1765..>2303
                     /locus_tag="PLH57_08200"
     CDS             1765..>2303
                     /locus_tag="PLH57_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02633.1"
                     /translation="MLIRAAILLIASLLSVQVFAAKKPPKKPEIQPIPGTDVARVLSE
                     AECVLVLNQDPSEPQSELKYFKSIKLLHTAELVKLGDDLTTSVRNSPSGRFVAKGTIP
                     GTLSVASPLGGNPVILQAHIGAISQIEFDHDATRMLTLGGEDQEVKLFSYREGEQAAE
                     IVPLATLAFERQKNWGNDT"
BASE COUNT          681 a          509 c          563 g          550 t
ORIGIN      
        1 aaagaactag aaagtaaatt ggaaattctc agaaaagatt ctgaaacgtt aattgccagt
       61 cgagaaaaca agattttaga tctaaagcga aaagtcgatc tattggaatt caattacgat
      121 gttctacaag ataaaaatgc catcgaacgc ggcaatgtgc agaaggcaaa cgaaaaaatt
      181 gagcgcgtac tgaaagtgct acggcttgca ttaggcatga tcgaaagtga cgatttgacc
      241 ggtgaaactg gggcttcaga gcaaaaggac aaggacgtac aagtttccta agtaatataa
      301 gggcgtacga tgttgaataa taaattgaac attctcatcg ttgaaagtga tacgcgccag
      361 accgagctca tggttgattt aatcaatgac gtcgcgtttc gccctagtgt agacaccact
      421 aactctccgg agcgttctct cgaactaata ggacgatcta attaccagtt ggttataatg
      481 gataccggaa ccggcaaggt cgacggtgtt tcaattcttg aacgtataaa aagcattagc
      541 ccctctactt cggtgatcat gacttcaagc ttcgccacga tcgaagaagc ggttaaggcc
      601 gttaggttgg gggcagacga atactttaaa aaacccttta atccggaaca attcaagcta
      661 gcggttcgtc gcgccttgga ccgccgcgat ctttattcag gcgatgaggc tgtagtcggc
      721 ctaatgaatt tgctcaactg ctgtcagttg atatctggct gtctcgaaga agaaaaaatt
      781 tttgatacgg ttctgggcta cttgaaacgc gagacccact cgcaagcagc agcttgtttt
      841 atttatacca ataataaaaa aaaatcagaa ccaaagcgca tcgaactctc taatgagcaa
      901 gatgctgatg tagttgaagt tatggtcagt agcgtgaatg taatccaaag ccacgccacg
      961 gaaccaggtt caatgaaaat tcttccaaaa gcgcaaggca accccgaaat agtgattttt
     1021 cgattcgctt ggaacgaaga acgttcctac tatgtggtgt gtgcgagccc aacttgggct
     1081 atttcggtcg acgaagtaga ttcacgcgtg cgccttcttc aaagtcaaat acacatgacc
     1141 gcgcgaaaca tcgacagtta tcgcgacgta caacacttgt taaatctcga cgaacctacg
     1201 ggattgttta atacccgtta ccttcataag agcctggaag atcttttcgc aaaacgaaag
     1261 gccggagaaa agagcacctt ctctgttttg tttgttgatg tagataagtt taagtcggtc
     1321 aacgaccagt acggtcattt gataggcacc aaattgcttc acgaaattgg tcagattacc
     1381 cgagggtgtt tgcgcatggg agatctggcg tttcgttacg gtggcgacga gttcgtcgta
     1441 attcttaaaa actgtccgac caaagccgca atagatattg ctgagcgttt aagggcagga
     1501 gttgaaaaga acgaatttct aagtaaagaa aaactgaacg tcagattgac ggtgagtatc
     1561 ggagtggcca attgcccaga gcacgctact tcgaaacggg caatcattga ggccgccgat
     1621 gccgcgatgt acgctgtaaa gcgtagtacc cgcaaccgag tttatgtcgc cgaactcaag
     1681 gccgcctgaa aattaaacaa ttcccgacgg cgtttgacaa cgattccccc gattggtatt
     1741 gcgatgcgac accggggggt tggaatgttg attcgagcgg ccatattgtt gattgcttct
     1801 ctgctgtctg tgcaagtttt cgcggccaaa aaaccaccaa aaaaaccgga aatacagcct
     1861 atcccaggca ctgatgtcgc gcgcgttttg agcgaggccg agtgcgtact ggtgttaaat
     1921 caggacccta gcgaacctca gtctgaactc aaatatttca agtcgatcaa actgctgcac
     1981 accgcagaac tagtaaaatt gggtgatgac ctcactacca gcgttcgaaa ttctccgagt
     2041 ggaagattcg tggccaaagg gacgattcca ggcactctct ctgtcgcatc gccacttggc
     2101 ggtaacccag tgatcctcca ggcccatatt ggcgcgataa gtcagataga gttcgatcac
     2161 gatgcaacgc ggatgctcac cctgggcgga gaggatcaag aagttaaact gttcagttac
     2221 cgagaagggg aacaggcagc ggaaatagtg ccacttgcga cattagcttt cgagcgccaa
     2281 aaaaactggg gcaacgacac aga
//
LOCUS       DAOMXO010000387         2299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27177_length_2299_cov_5.337344, whole genome shotgun sequence.
ACCESSION   DAOMXO010000387 DAOMXO010000000
VERSION     DAOMXO010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27177_length_2299_cov_5.337344"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PLH57_08205"
     CDS             complement(<1..895)
                     /locus_tag="PLH57_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02634.1"
                     /translation="MKATDLRIALIKVVVGCTIFSTVLSHAAQEKAGAFNISERRAVK
                     QNLELTTGIQKTVDLNFDPCPNLQDCVRVTNSDLFKIQYSKDKRQLIFTPVKDGDTTV
                     TIRDQNGDIRLILNTLVLKANLSRRIGELKHLLRDVEGIEIKPLGDKILVDGEVVVMN
                     DLNRLYAVLSDESYKSFVLNLVGLSPLGMQVLAEKIQAEINNPGIRVRILNSLFVVEG
                     QVDNIDQANTALDVARNMIQGFLLPNFNYEGRNPFDVKKTLTKDPVVPRITIAKRKDP
                     PPPKLIRITVDFVELSKDYLRN"
     gene            complement(908..1696)
                     /gene="cpaB"
                     /locus_tag="PLH57_08210"
     CDS             complement(908..1696)
                     /gene="cpaB"
                     /locus_tag="PLH57_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HRK02635.1"
                     /translation="MNNKAFTFSLALALLATVMVYSYMTSKEEEWQTKYGTPTAVVVA
                     KEDIKELETIDETKIEILKKPQQYVEPGALTSKDEVVGFIASVPLKRGEQVTLSKAMT
                     PGLRTGLARQVSPGKRAIALSVRDDNAVGRLIKPGDRIDVLAIIQPPGQRGSDIAKLV
                     LQDVLILAVGEYITTLPARKVQKDEMSGKVITKNLNLDRNFNTITLEVDPNSAQQVLL
                     LQSMGMGLYVMLRNNDDTERVALGASTLPEILNSGGTRGVATQR"
     gene            complement(1730..>2299)
                     /locus_tag="PLH57_08215"
     CDS             complement(1730..>2299)
                     /locus_tag="PLH57_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRK02636.1"
                     /translation="KWPRNERYMTMAAKSARLGVGKNKLKRGARGQSVVEFALLLPVL
                     VGLFYILIQANSAITTAIVHQKYVRMRLHFMALNHRYYPQLDTLSGRASDNVFQRWWI
                     GIGDPVTYGLIDPPTRAPEISIGRVSVAEDQMPKEEYPSIQRRQKVRVRVAAFTCLAP
                     TAAKFGQNYSEGYLGEETFAGGNYRYCQR"
BASE COUNT          566 a          571 c          512 g          650 t
ORIGIN      
        1 aattacgtaa gtagtctttt gacaattcca cgaagtcgac tgtgatacga ataagcttgg
       61 gcggtggtgg atccttccgt tttgcaatgg taatgcgagg taccaccggg tccttggtta
      121 aagttttctt cacgtcaaat gggtttctac cttcatagtt aaaattcggg agtaaaaagc
      181 cttgaatcat attgcgagca acgtcgagcg cagtattcgc ttgatcaata ttgtcgacct
      241 ggccttcaac cacaaaaaga ctattcaaga tacgaacccg aattcccgga ttgttgattt
      301 cggcttgaat tttttccgct aatacttgca taccgagcgg gcttaaccct acgagattga
      361 gtacaaaact tttatacgac tcatcgctta ggaccgcgta tagtcgattg agatcgttca
      421 ttacgaccac ttcgccatct actaaaattt tgtcgcccag aggtttaatt tcaatgccct
      481 caacatcgcg aagcaaatgt tttagctccc caattcgacg cgataggttc gccttaagca
      541 ccagagtatt tagaatcaaa cgaatatcgc cgttttgatc acgaatcgtt acggtggtgt
      601 cgccgtcttt cactggcgta aaaatgagct gtcgtttgtc tttggaatac tgaattttaa
      661 ataggtctga gttggtgact cgcacgcaat cttgcaaatt gggacaagga tcaaaattta
      721 ggtctaccgt tttttgtata cccgtagtga gttcgaggtt ttgttttact gcccggcgtt
      781 cagaaatatt aaatgcgccg gccttctcct gagccgcgtg actcagaacg gtcgaaaaaa
      841 ttgtgcatcc aaccacaact tttattaggg cgattcgcaa gtccgttgct ttcattttct
      901 cacccaatta tcgctgcgta gcgactcctc tcgtaccacc actgtttaaa atctcaggaa
      961 gagttgacgc tcccaacgcg actcgttccg tgtcgtcgtt gttacgcaac atcacatata
     1021 accccattcc catactttgc agtaacagaa cttgttgtgc ggaattgggg tccacttcga
     1081 gtgtgatcgt attgaaatta cgatcaagat ttaaattttt tgtaatcacc ttaccgctca
     1141 tctcgtcttt ttgaacttta cgagctggaa gggttgttat gtattcgcca accgcgagaa
     1201 ttaaaacgtc ctgtaaaacg agtttggcaa tgtcggagcc cctttggccg ggcggttgaa
     1261 taatggccaa cacgtcgatt cggtcgccgg gcttgatcag gcgaccaact gcgttgtcgt
     1321 cgcgcactga taaagcaatg gcgcgcttgc cgggcgacac ctgccgcgcg agaccagtgc
     1381 gcaaccccgg tgtcattgcc ttcgaaagtg tcacctgttc accgcgcttg agcggcaccg
     1441 atgcgataaa tccaaccact tcatctttcg aggtcaaggc acctggttcg acgtactgtt
     1501 gtggtttttt tagaatctcg attttggttt catcaattgt ttcgagttct ttaatatctt
     1561 ccttagcaac tacgactgcc gtaggggtgc catacttggt ctgccactcc tcctctttcg
     1621 aggtcatgta gctatagacc atcaccgttg caagcaacgc gagtgctaaa ctaaatgtga
     1681 atgccttgtt attcatctag tacttgaaaa cgaaggcagg gccaaaagac tatctttgac
     1741 aataccgata gttgccgccc gcgaacgttt cctctcctag atacccttcg gagtaattct
     1801 gcccaaactt agcggcagtg ggggctaaac aggtaaatgc tgcaactcga acgcgcactt
     1861 tttgccggcg ctggatggaa ggatattcct cctttggcat ttgatcttct gctacgctca
     1921 cccgaccgat cgatatttcg ggcgctcggg ttgggggatc aataagtccg taggtaaccg
     1981 gatcgccaat accgatccac cagcgttgga acacgttatc actcgctcgg cccgacaaag
     2041 tatccaactg tggataatat cggtgattaa gcgccataaa atgcaatcgc attcgaacat
     2101 acttttggtg cacgatcgca gtggtgatgg cggaatttgc ctggatcaaa atataaaaaa
     2161 gccccaccag taccggcaac aacaaggcaa actctacgac actctgccct cttgcgcctc
     2221 gctttaattt atttttaccc actcctaacc gcgcagattt agcagccatt gtcatatagc
     2281 gctcgtttcg cggccattt
//
LOCUS       DAOMXO010000388         2293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27266_length_2293_cov_4.320822, whole genome shotgun sequence.
ACCESSION   DAOMXO010000388 DAOMXO010000000
VERSION     DAOMXO010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27266_length_2293_cov_4.320822"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..693
                     /gene="dcd"
                     /locus_tag="PLH57_08220"
     CDS             127..693
                     /gene="dcd"
                     /locus_tag="PLH57_08220"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRK02637.1"
                     /translation="MSIKSDRWITKMALEHGMIEPFEPRQMRKSDKTPIISYGVSSYG
                     YDVRVADEYKIFTNVNNTLVDPKNFDPNSFVSYKGDVCIVPPNSFALARTIEYFRIPR
                     NVLTICLGKSTYARCGIIVNVTPFEPEWEGIVTLEISNTTPLPAKIYSNEGIAQVLFF
                     ESDEPCEVSYKDKNGKYQAQRGVTLPRM"
     gene            711..2114
                     /locus_tag="PLH57_08225"
     CDS             711..2114
                     /locus_tag="PLH57_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRK02638.1"
                     /translation="MASFNSLTAAMIAELKSVLSSPQILLQDAEAIEKYSHDFTEDLR
                     FSPNCAVLARNRDDVVATLKFANKHRIPVTPRGAGTGLSGAALPVFGGIVLSLEKMNR
                     ILEIDENNLVAVVEPGVITEILQNAVQEKGLYYPPDPASRGSCMLGGNLSHCAGGPHA
                     VKYGVTKDYIYGVEAVLPSGEIIEHGGKLLKNSTAYNLTQLLVGSEGTLAVVTKIFLK
                     LVPNPTHKALVLAPFRTLEDPARAVAALFQAGLTPSACEYMEKSAVEAGAKQTSQPNP
                     FPWAEAMLLIEVDGFSQTEIERQLEKLAEVLDRFGAADMALAETPEQQAKLWRLRRSI
                     GEAVKTRAIYKEEDTVVPRFALPELLMSVKKICARYGVANVCYGHAGDGNLHVNLLKE
                     NLSDDAWVRELPECITEIFQEVKRLGGTVSGEHGVGYVQRAYLPIVYGEAELALMKRL
                     KFAFDPNGILNPGKIFK"
     gene            complement(2107..>2293)
                     /locus_tag="PLH57_08230"
     CDS             complement(2107..>2293)
                     /locus_tag="PLH57_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02639.1"
                     /translation="RTFGKLCASDRSQEMSYQFVLWVLVLFVGMIIDPLRWPAVQVIA
                     TILVQRVWLWAWRRGII"
BASE COUNT          633 a          561 c          537 g          562 t
ORIGIN      
        1 aaatcctcct tgattcaagc aacaatggat ctctcaaaat tgttcgccca gaacgatttc
       61 aagtcaaaca atttttagga tttgttttga tttatgttag ttctgaaatc gggaggacct
      121 ctgactatgt cgattaaatc agatcgctgg atcaccaaaa tggcactcga acacggaatg
      181 atcgaaccct ttgaaccgcg acagatgcgg aaatccgaca agaccccgat tatcagttat
      241 ggggtttcct catacggcta cgatgtacga gtggccgatg aatataaaat ttttacaaat
      301 gttaacaata ctctagttga ccccaaaaac ttcgatccca attcgtttgt tagctacaaa
      361 ggtgacgtgt gcattgtgcc gcccaactct tttgcccttg ctcggacgat cgaatatttt
      421 cgtattcccc gaaacgtact tacgatttgt ttaggaaaaa gcacctacgc gcgctgcggc
      481 atcattgtaa atgtaacgcc ttttgagcca gagtgggaag gtatcgtgac gctcgagatc
      541 agcaacacta ctccactgcc ggctaagatc tactccaacg agggaatcgc ccaggtacta
      601 tttttcgaaa gtgacgagcc ttgcgaggtc tcctacaaag acaaaaacgg aaaataccaa
      661 gcacaaagag gcgtgactct gccgcgaatg tgacgtaaat ttggcgccct atggcatctt
      721 tcaactcgtt gaccgcggcc atgatcgcgg aactaaaaag cgttctaagt tcgccgcaga
      781 ttctgttgca agacgctgaa gcgatcgaaa aatactcgca cgacttcaca gaagacctgc
      841 gtttttcgcc aaactgtgcc gtacttgcac gaaaccgtga cgacgtcgtt gcgactttga
      901 agtttgcaaa caaacaccga attcctgtaa caccacgcgg cgcgggaact ggcttaagcg
      961 gcgcggcgct tcccgtcttt ggcggcattg tactttccct cgaaaaaatg aaccgcattt
     1021 tagaaatcga cgaaaacaac ctggtcgcgg tggtagagcc cggtgtaatc accgagattt
     1081 tgcaaaacgc tgtccaagaa aaagggctct actacccacc cgatcccgcc agtcgcggca
     1141 gctgtatgtt aggcggcaat ctttcgcact gtgccggtgg accacatgcg gtgaaatacg
     1201 gcgtaaccaa ggactatatt tacggcgtcg aagccgtact cccttcgggc gaaattattg
     1261 aacacggcgg caaattattg aaaaattcga cggcctacaa tctcacccaa cttctagttg
     1321 gttccgaagg tactcttgcg gtagttacca agatttttct caagttagtt ccgaacccca
     1381 cccacaaagc cttggtttta gcgccatttc gtactctcga agatccggca cgtgcggtag
     1441 ccgctttatt tcaggctggc cttacaccca gtgcttgcga gtacatggaa aaaagcgcag
     1501 ttgaagcagg cgccaaacaa acttcccagc cgaatccttt cccttgggcc gaagccatgt
     1561 tgttgattga agtagatggt ttcagccaga cagaaattga gcgacaactc gaaaaattgg
     1621 ctgaagtgtt ggatcggttt ggagcagccg acatggcttt agcggaaact cccgaacaac
     1681 aagccaagtt atggcgtctt cgtcgctcaa tcggtgaagc tgttaagacg cgagcaattt
     1741 ataaagaaga agataccgtt gtacctcgtt tcgctctccc cgaattgctc atgtcggtta
     1801 aaaaaatctg tgctcgttat ggtgttgcca atgtttgtta cggtcacgcg ggcgacggaa
     1861 atttacatgt aaatttgctg aaggaaaatt taagtgacga cgcttgggta cgagagcttc
     1921 cagagtgtat caccgaaatt tttcaagaag ttaaacgcct tgggggtaca gtcagtggcg
     1981 aacatggcgt tggttacgtt caacgtgcct acttacctat cgtttatggc gaggcagaac
     2041 ttgcgcttat gaagcggctg aagtttgcgt tcgatccgaa cggaattttg aatccaggaa
     2101 aaattttcaa ataattccgc gtcgccaggc ccacagccaa acgcgttgaa ctaaaatcgt
     2161 tgctatcact tgcacggcag gccagcgaag cggatcgata atcatgccca caaacaaaac
     2221 caaaacccaa agaacgaact ggtaactcat ctcttgcgat cgatcagagg cacaaagttt
     2281 tccgaaggta cga
//
LOCUS       DAOMXO010000389         2292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27275_length_2292_cov_5.974519, whole genome shotgun sequence.
ACCESSION   DAOMXO010000389 DAOMXO010000000
VERSION     DAOMXO010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27275_length_2292_cov_5.974519"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLH57_08235"
     CDS             <1..663
                     /locus_tag="PLH57_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02640.1"
                     /translation="VSVVFGIGKWQSNKSLGIAPPSTPKLDLYRNTGISAAALESSRK
                     EPLPAMEAQKTALSLARKVLKSREDLRNLEKILEKGGPNNLVVQAKKTLLELRIRSDN
                     YEAEAEAIQKRMDAIEILLVAVQSGPEPIKQESIRAILSVLSTPPVATKNSAGKVNSI
                     LGRKADIADRVELYRILKFLDPKLVAELEHSVANSDLQRIYGLAKAIDADISKNIKEK
                     SI"
     gene            660..1181
                     /locus_tag="PLH57_08240"
     CDS             660..1181
                     /locus_tag="PLH57_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02641.1"
                     /translation="MKKFRTMKTILAVLLLTAVPSAGRAATIQLGDEFETSTTHFVVT
                     DIKECDKKSKSAQSAYDLTMEEVLELEFADPMADGAVLSCKTKETVEFAIGIAQLSIA
                     TGSVIAMCSAIVAPFGAVIAGAAVAVQFVDFALDKVDCYDEASEEKNRALIKQQICEA
                     MIANGLECDPNKV"
     gene            1191..1484
                     /locus_tag="PLH57_08245"
     CDS             1191..1484
                     /locus_tag="PLH57_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02642.1"
                     /translation="MNNLVKDLTNNNKKHGLIVAAAFFTVPVSVILIVDRSGDLIKWI
                     GWPEILASQTYEFRGYLGFLILHIALITTGVIFGRNGLRNQLRAKYKDIVQPQ"
     gene            complement(1495..2175)
                     /locus_tag="PLH57_08250"
     CDS             complement(1495..2175)
                     /locus_tag="PLH57_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRK02643.1"
                     /translation="MFSSGNRCTCKATEVKLKQMTHDLRSPVAALGFSIEHLLRDSCF
                     RGPAENVMRASLERIRAILEDARVACSRSATDVELNQVLDLISREKEQEYRGKLRVRL
                     VNRRSGKIEIKAHKSGLERMLSNVLNNAAEALINQNPKSEGPLVVGIEVVTESNLVGI
                     RVSDGGPGMPDEVLERLGKVPISFGKKDGMGLGLYHAAQTLQSWGGRLEVQSTDAGTD
                     VTLWVPRV"
BASE COUNT          628 a          584 c          517 g          563 t
ORIGIN      
        1 gtttctgtgg tttttggaat aggaaagtgg cagtcgaata aatcgctggg aatcgccccg
       61 ccatcgacgc ccaaactcga cctctaccga aacaccggta ttagtgcggc tgcgctcgag
      121 tcttcccgga aagaaccttt accggcaatg gaagcccaga aaacggccct ctccttagct
      181 agaaaggtgc taaaaagccg cgaagactta agaaatctcg aaaaaattct cgaaaagggc
      241 ggcccgaata atctcgtcgt ccaagcaaaa aaaacattac tcgaactacg cattcgctca
      301 gacaactatg aggctgaagc ggaagctatt caaaaaagaa tggatgcgat tgaaattcta
      361 ttagtcgcag tccaaagcgg accggaacct ataaaacaag agagtattcg agccatactc
      421 tcagtcctat cgacgcctcc cgtagcgact aaaaattcag cgggcaaagt caattcgata
      481 ctcggccgaa aagccgacat cgccgaccgt gtcgaactct accgcattct taaatttctg
      541 gaccccaaac tggtcgcaga actcgagcac tcggtcgcga atagcgatct tcaaagaatt
      601 tacggactgg ctaaagctat cgatgccgat atttcaaaaa acattaagga gaagtctata
      661 tgaagaagtt ccgaacaatg aaaacgatcc tagcggtatt gctgctaact gcggtaccat
      721 ccgcggggcg cgccgctacc attcaattag gtgacgaatt cgaaaccagc acgacccact
      781 tcgtggttac cgacattaag gagtgcgata aaaaatcgaa atcagcgcaa tccgcttacg
      841 acctgacaat ggaagaagta ttagagctcg aattcgccga tcccatggca gacggcgcgg
      901 tactaagctg caaaacgaaa gaaacggtgg agttcgccat cggcattgct cagctttcca
      961 tagcgaccgg ctctgtcatc gcgatgtgca gtgccattgt cgctccgttt ggagcagtga
     1021 tagcgggcgc tgcagtggcg gttcaatttg ttgatttcgc attagacaaa gtagattgtt
     1081 acgacgaagc cagcgaagaa aaaaatcgtg ctctgattaa acaacaaatt tgtgaagcga
     1141 tgatcgcaaa cggtctcgag tgcgatccga acaaagttta aggagtaaat atgaataacc
     1201 ttgttaaaga tctaacgaac aacaacaaaa aacatggcct aattgtagct gctgcttttt
     1261 tcacagtacc agtcagtgtg attctaatag tggaccgaag cggcgacttg ataaaatgga
     1321 tcgggtggcc tgaaatcctc gcgtcccaaa cctatgaatt tcgcggatac cttggatttt
     1381 tgatcctgca catcgcctta attacgacgg gagtgatctt tgggcgcaac ggattaagaa
     1441 atcaattgcg ggctaaatac aaagacatcg ttcaaccgca gtaactttct gtcgctagac
     1501 gcgtggaacc cacaaagtga cgtcggttcc cgcgtctgtc gactgaactt cgagcctgcc
     1561 gccccagctt tgtagcgttt gcgctgcgtg ataaagaccc agccccattc cgtctttctt
     1621 accaaaactt attggcactt taccgaggcg ctccagtact tcgtcgggca tacccggtcc
     1681 accgtcggac acgcgaatac ccaccagatt cgattcagtg accacctcta ttccaacgac
     1741 caacggccct tcgcttttcg gattttgatt aattagtgcc tccgcggcat tattcagaac
     1801 gttcgacaac atccgctcga gtccactttt gtgggctttg atttcgattt tgccgctcct
     1861 tcggttgacc aatcgcacgc gtaactttcc acgatattcc tgttcctttt ctcgcgagat
     1921 taggtctaac acttgattta attcgacgtc ggtcgcgctc cgagaacagg ccactcgagc
     1981 gtcctctaat atagcgcgta ttcgctcgag cgacgctcgc attacgtttt cggcgggacc
     2041 tctaaaacaa gaatccctta gcaagtgttc tatcgagaac cctaatgccg cgaccggcga
     2101 tctcaaatcg tgagtcattt gtttgagttt tacttcggta gccttgcagg tacagcgatt
     2161 tccagagcta aacatagtgt gcgtcccctt cgttggttcc ttctatttca atttctgggc
     2221 cgcactttcc gcagtctaaa tcattgatat atttgaatta attttcatac ctacctaaaa
     2281 aaagctgtcg aa
//
LOCUS       DAOMXO010000390         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27546_length_2275_cov_5.741441, whole genome shotgun sequence.
ACCESSION   DAOMXO010000390 DAOMXO010000000
VERSION     DAOMXO010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27546_length_2275_cov_5.741441"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLH57_08255"
     CDS             <1..192
                     /locus_tag="PLH57_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02644.1"
                     /translation="ETQYMGFGYLTLSDWGGGFNWSGPFTQSLKNWAADKEWFGFADV
                     NDSSEEAQIETIKKFFGKK"
     gene            550..864
                     /locus_tag="PLH57_08260"
     CDS             550..864
                     /locus_tag="PLH57_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02645.1"
                     /translation="MLKRTSKNLILALVVGTPMVVASVPKSREISNDTSIYQNLREVR
                     LSPVKHTDLEARIDAGFEKLSATEKQHRTRVLPMRVSGPMDRVMKTPYSPSFKSHKRD
                     RR"
     gene            997..2259
                     /locus_tag="PLH57_08265"
     CDS             997..2259
                     /locus_tag="PLH57_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRK02646.1"
                     /translation="MTRGAYKVADERLAEQGRLKIEWAESRMPVLMKLREKYAKTQPL
                     KGQRIAGCLHVTKETAVLVRTLKAAGAEVSWSGCNPLSTQDDIAAALAAEGISIYAWH
                     GMNKEEFYWCIDRTLDFKPTLTLDDGADLIFTVHSKYKELCSTIIGGTEETTTGVHRL
                     RAMAEDGALKYPVIAINDAITKWDFDNVFGTGQSSIDGILRATSVMLAGKTFVVAGFG
                     HCGRGCAMRARGMGARVVVTEILPTSALKATLEGYEVMTMDEAAKIGDIFITATGMKD
                     IIVDRHFRSMKDGAIVCNTGHYDCEINLEQLGKLAKRVREVRPNNEEHTMADGRRIYV
                     LAKGRLVNLAAAEGHPSEVMDMSFANQFQGMLRLAREGKTMKPNVYTLDPELDQEIAG
                     IKLETSGIKIDHLTAEQEKYARDYSAGT"
BASE COUNT          608 a          524 c          598 g          545 t
ORIGIN      
        1 gaaactcaat acatgggttt tggttattta accctcagtg attggggcgg aggcttcaac
       61 tggagcggcc cttttaccca aagcttaaaa aactgggcgg ccgataaaga gtggtttgga
      121 tttgccgacg tgaatgactc ttcggaagaa gcccaaatag aaacgattaa gaagtttttc
      181 ggaaaaaaat agtagcttcc ttgagttttt aatttcggct agagccaggc gagccacacg
      241 agccaggcac tcatggctat caatgtaatg gcacttgcgc gtgcatggca gtggtcggtg
      301 tgggtttttt gaggaccaaa atctggtcta atgttcatat accctaaagt agcaagtcat
      361 gtgccgtgtc agcagcgcct aagggggcag ggtcgccgcc gccctttggc tagggtggct
      421 tgacgagtga ctaaaagttc gacgattctc gagagcagcc cttagggaac ccctgggagc
      481 gacttttggc acctcatttg cttatggagc catcagcgtt tttaagaaag gcgctgttgg
      541 aggttcatta tgttgaagcg cacgtctaaa aatttgatct tagctctggt agttggaaca
      601 cccatggtcg tggccagtgt gccgaagtcg cgtgaaattt cgaacgacac gtcgatttat
      661 caaaatctgc gagaagttcg cctgagtcca gtgaagcaca ctgacctcga ggcgcgcatt
      721 gatgcagggt tcgaaaaact ctccgcgacg gaaaaacaac accgcacgcg agtattgccg
      781 atgcgggtat ctggtccgat ggaccgggtt atgaaaaccc cttattcgcc gagctttaaa
      841 tcgcataagc gcgatcggcg ttaggtccta ccgcccatag cggacgaggt gccccccgcc
      901 ccgggtccta tggtcccggg gggcggcccc aacgagcacg tgacattttc ggccgcatct
      961 aataggttca cttcatattt attaaggaga tgaaccatga cccgtggagc ttataaagtt
     1021 gccgatgaac gccttgcaga acaaggccgt ctaaaaattg aatgggccga gagccgtatg
     1081 cccgtgctca tgaagttgcg tgaaaaatac gcaaaaactc aaccactcaa aggtcaacgc
     1141 attgcgggtt gtttgcatgt tacgaaggaa accgctgtgc ttgttcgcac gctgaaagct
     1201 gctggcgcag aagttagctg gtctggctgt aatccacttt cgactcaaga tgatattgca
     1261 gcagcactcg ccgcagaagg cattagcatt tatgcatggc atggcatgaa taaagaagag
     1321 ttttactggt gcatcgatag aactcttgat ttcaaaccca cgcttacgct cgacgacggt
     1381 gcagatttaa ttttcaccgt tcactccaaa tacaaagaac tttgttcgac cattattgga
     1441 ggaacagaag agaccaccac cggtgtacac cgtttgcgtg caatggcaga agacggagct
     1501 ctcaagtacc cagtaatcgc catcaacgat gcgatcacta agtgggattt tgataacgta
     1561 tttggcaccg gacaatcttc gatcgacggc atcttgcgtg caacgagtgt aatgctcgcg
     1621 ggtaaaactt tcgtagttgc aggatttggc cactgcggcc gtggttgcgc aatgcgcgct
     1681 cgtggcatgg gagcccgggt tgtagttact gaaattcttc ctacttcggc gctcaaggca
     1741 acccttgaag gttacgaagt gatgactatg gacgaagctg cgaagattgg cgacatcttc
     1801 attaccgcaa ctggaatgaa agatattatt gtcgatcgac acttccgttc catgaaagat
     1861 ggcgcgattg tttgcaatac tggtcactac gattgcgaaa tcaatttgga acaactcgga
     1921 aagttagcaa agcgagtgcg tgaagttcga ccgaacaatg aagaacacac gatggcagat
     1981 ggtcgtcgca tatatgttct tgccaagggc cgactagtaa atcttgcggc ggcagaaggt
     2041 cacccttctg aagtaatgga catgagcttt gcaaaccaat ttcaaggaat gttgcgtttg
     2101 gcgcgcgaag gcaaaaccat gaaaccaaac gtttacacct tggacccaga gcttgatcaa
     2161 gaaatcgccg gaattaagct cgaaacaagc ggaattaaaa tagatcatct caccgccgag
     2221 caagaaaagt acgctcgcga ttattctgca ggtacttgat agggtattaa cattc
//
LOCUS       DAOMXO010000391         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27548_length_2275_cov_4.881982, whole genome shotgun sequence.
ACCESSION   DAOMXO010000391 DAOMXO010000000
VERSION     DAOMXO010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27548_length_2275_cov_4.881982"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLH57_08270"
     CDS             complement(<1..829)
                     /locus_tag="PLH57_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02647.1"
                     /translation="MKKQVRFGLGVLGILMLVSCTRSTAYRDDQDYYRRDGGSATSRA
                     SKFGQPKKKIFFYSFHNDTPFLADGLGDFMVSELKREVRTSGKGVVPDDLRPKETSKD
                     FYSGDKIRLSPLIREAKRMGVAMVAVGKIKKIIYRRKGDEVGLFRKKLSVAAVDLEMR
                     VFSVGDNKELLFVEKSVDAESSEVDFVEDEAEDPKSQRVELIKMALRNGAHQFGTDIN
                     LMLDKISWEGRIAKIGGGKIYINAGRASGILVGDILKVLTPGEDVYDPATNAYLGRSQ
                     "
     gene            complement(840..1910)
                     /locus_tag="PLH57_08275"
     CDS             complement(840..1910)
                     /locus_tag="PLH57_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02648.1"
                     /translation="MIKLLQAKMRTLTFIVGVVVANAAIAQTPKPVKTTKTFDEWVLE
                     AIQKTYSELKSSGRTAPGADTAFNGEVLSAAMVFVRNYEVTRETTSVDVDRDRLRGFL
                     NFSRDTFTNRPDVSICVGVRRLADCAMCGDFALEIQKGVTALLNKRGVQAVAGPEFAS
                     DSTLTGERAFEDYALRAQGQGCDFALLSTISASSDASVEAGSLKIDTALLMRDRRQRS
                     LRAQSTFKISEAAVLELTTNKTPKLAVNVVAEVLAVAANRAKEEVVNVGLGQEGFLKI
                     ENLPNYATLLVIKNRLNLLVKGLELKERFLTPGAIEFVYPISFKEDLHHEAKREANQF
                     GGIGGVNAEVKGDSTGLTIRIL"
     gene            complement(1929..>2275)
                     /locus_tag="PLH57_08280"
     CDS             complement(1929..>2275)
                     /locus_tag="PLH57_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02649.1"
                     /translation="VPLIISGNIGLEEETAKIFFRTIDRVEEYQNKRINGVTIRLNPS
                     QVNPHVLAQLRAVLVRHRGKCAIALEYSGELGGARFRSRHQLPKELYTTPSTQMAHEV
                     NALFGGDVVKFV"
BASE COUNT          534 a          648 c          493 g          600 t
ORIGIN      
        1 cttgactgcg tcctaggtag gcattggtcg cggggtcgta gacatcttcg cccggcgtga
       61 gaacctttaa aatgtcaccc accaaaattc cggaggcccg cccagcatta atgtaaattt
      121 ttccaccgcc aattttggca atgcgccctt cccaactgat cttatcgagc atgagattga
      181 tgtccgtacc aaattggtgc gcaccgttgc gaagcgccat ttttataagc tccactcgct
      241 gactctttgg gtcctcggct tcgtcctcta caaaatctac ttcactactt tcagcatcga
      301 ccgacttctc gacgaacaag agttctttat tatctcctac gctaaacact cgcatttcta
      361 agtccaccgc ggcgaccgag agtttttttc ggaacaaccc gacttcgtcg cctttccggc
      421 gataaataat ctttttaatt tttccgaccg ccaccattgc cactcccatg cgcttggctt
      481 cacgaatcag cgggctaagc cggatcttat cgccgctgta aaaatcttta gacgtttcct
      541 tgggccgcag atcgtcgggc accacgcctt ttcccgaggt tcttacttcg cgtttaagtt
      601 ccgacaccat gaagtcgcca agaccgtcag ccaaaaatgg agtgtcgttg tgaaaggaat
      661 aaaaaaagat cttctttttt ggctgaccaa actttgaagc ccgcgaagtg gccgacccac
      721 catcgcggcg atagtaatct tggtcatcgc gatatgcggt ggaacgcgtg caagagacca
      781 acattaggat acctaataca ccaagtccaa agcgcacttg ttttttcata tttgattcct
      841 cacaaaatcc ggatcgttag acccgtacta tcgcctttaa cttctgcgtt cacgcccccg
      901 atgccgccga attgattggc ctctcgcttg gcttcatggt gcaggtcttc tttaaaactt
      961 attggatata cgaactctat cgccccaggt gttaaaaatc tctctttgag ttccagtcct
     1021 tttactaaca agttcaatcg attttttatg acgagtagcg tggcataatt cggaagattc
     1081 tcgattttca aaaaaccttc ctgaccaaga ccaacgttca caacctcttc tttagctcgg
     1141 ttggctgcca ccgccagaac ctcggcaact acgttcaccg ctaacttcgg agttttatta
     1201 gtcgtcaact ctagcacggc tgcctccgaa atcttaaacg tactttgagc tcttaacgat
     1261 cgttgacgtc gatcacgcat caatagtgct gtgtcgattt tgagcgaacc cgcctccacc
     1321 gaggcatccg acgaagccga gatagtactc aatagcgcga aatcgcaccc ctgcccctgc
     1381 gcgcgcaacg catagtcttc aaaagctcgt tcaccggtga gcgtcgaatc gctcgcgaat
     1441 tctggcccag ctaccgcttg cacgccgcgt ttattcagca atgcggtaac gcctttctgt
     1501 atttcgagcg caaaatctcc gcacattgca cagtctgcca aacgccgaac tcccacacaa
     1561 atcgatacgt ctgggcgatt tgtgaaagta tcgcgtgaaa agttcaaaaa cccgcgaaga
     1621 cgatcgcggt ccacgtcaac cgacgttgtc tcgcgggtga cttcataatt gcgaacaaaa
     1681 accatggccg ccgataaaac ttcgccgtta aacgcagtgt cggcacccgg agcggttcgc
     1741 cctgaactct tgagttcgct gtaggttttc tgaatcgcct cgagcaccca ttcgtcaaac
     1801 gtcttggtcg tttttaccgg tttcggtgtc tgagcaatcg cggcgtttgc cacaactacc
     1861 ccgactatga acgtaagggt tctcattttg gcttggagca acttaatcat acccctattt
     1921 caacacgact aaacgaactt cacaacgtcg ccgccaaaga gtgcgttcac ttcgtgggcc
     1981 atttgggtcg aaggggtggt atacagctct ttgggaagtt ggtggcgact tcgaaatcgt
     2041 gcgccgccca attcaccgct atactccaga gcgatggcac attttcctcg gtgccgcacc
     2101 agcactgccc gcagttgcgc caagacatgc gggttcacct gcgacggatt caggcgaatc
     2161 gtcacgccgt tgattctttt attctgatac tcctcaacgc gatcgatcgt tctaaaaaat
     2221 atttttgcgg tctcttcttc gagcccaatg ttgccagaaa ttattaacgg aacgc
//
LOCUS       DAOMXO010000392         2269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27649_length_2269_cov_6.325203, whole genome shotgun sequence.
ACCESSION   DAOMXO010000392 DAOMXO010000000
VERSION     DAOMXO010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27649_length_2269_cov_6.325203"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLH57_08285"
     CDS             <1..758
                     /locus_tag="PLH57_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRK02650.1"
                     /translation="LVTVLVGEDPASQIYVSKKGSTCHALGMAHEDFKLSSNISEGEL
                     LLLLDRLNADDSVDGILVQSPLPKQISSQRVYDHIRPDKDVDCFSATNVGLCAQNRAL
                     LLPCTPAGIMELLRFYKIAVAGKNALVLGRSDIVGKPMALLLLHADATVTVAHSKTSD
                     LAEHIANSDLVVSAIGRRGVLTGTLPWKSSCTVIDVGIHRIADGRVVGDCEFSEIEQK
                     VAAITPVPGGVGPMTIAWLMANTVKAAYFRKGN"
     gene            760..1869
                     /gene="gcvT"
                     /locus_tag="PLH57_08290"
     CDS             760..1869
                     /gene="gcvT"
                     /locus_tag="PLH57_08290"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRK02651.1"
                     /translation="MNTPKSNQLKRTSLYDEHVRLNGKIVDFAGWEMPVQYTGLADEH
                     DTVRNKVGLFDVSHMGEVRVRGRGSLEFLNKLLTNNVAKIQLGQAQYNVMCYEDGGCV
                     DDLIVHKMGEEDYFICVNASNTEKDFEWMRQHAPASLSLENVSSEYTQIAIQGRFAAE
                     TLAPLTDASLDSIKYYWFQQSSILGKPAIIARTGYTGEDGFEVYIPWDHGPKVWQTLL
                     ESGAGRGIKPCGLGARDTLRTEMKFALYGHEIEKDLNPLEAGLGWVVKLEKSDFIGAG
                     ALRRIQETGPKRALVGLKSLERGIPRQGYKVEHNGREVGLVTSGTMSPSLRVGIAIAY
                     VERDLSAVGTTLDVVIRDQKVRHEIVTTPFYKRPY"
     gene            1894..>2269
                     /gene="gcvH"
                     /locus_tag="PLH57_08295"
     CDS             1894..>2269
                     /gene="gcvH"
                     /locus_tag="PLH57_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRK02652.1"
                     /translation="MEFPSGLYYTKDHEWAKWDEKTGTVTVGITDHAQDSLGDIVYLE
                     LPQKGRSLKTHETFGVVESIKAVSDLYSPVSGEVVETNAKAAEDPSVLNRAAYTDGWL
                     MRLKVADSAALSGLMSSKDYEIY"
BASE COUNT          590 a          545 c          571 g          563 t
ORIGIN      
        1 agcttgtcac cgttttagtt ggagaggacc ccgcaagtca gatctatgtt tcgaaaaaag
       61 gttccacgtg tcacgccctt ggaatggctc acgaggactt taagttaagc tcaaacatct
      121 ccgagggcga attgctgttg cttctagatc gcctaaatgc tgacgattcg gtcgatggca
      181 ttttagttca aagtccactt cccaagcaga tttcgagtca acgggtttat gaccacattc
      241 gacctgataa agacgtcgac tgtttttctg ctaccaacgt tggtctttgt gcgcaaaatc
      301 gcgcattact actaccctgc actccggcgg gcatcatgga acttctgcgc ttttataaaa
      361 tagccgtagc tggaaaaaac gcactcgttt tgggtcgctc ggatatcgtt ggaaaaccaa
      421 tggccctttt gcttctgcac gcagatgcta ccgttactgt ggcccactcg aaaacctccg
      481 atcttgccga acatatcgct aactctgacc tggtcgtttc agccatcggg cgtagaggcg
      541 tgttgacggg gacgctcccc tggaaatctt cctgcacagt catcgatgtc gggattcatc
      601 gcattgcgga cggcagagtt gttggagact gcgaattttc agaaattgaa caaaaggtcg
      661 ccgctatcac tccggttccg ggcggtgtgg gccccatgac catcgcatgg ttgatggcca
      721 acactgttaa ggcggcctat tttcgcaaag gaaactgaag tgaacacacc gaaatcgaat
      781 caacttaaac ggacttcttt atatgacgaa cacgttaggc ttaacggcaa aatcgtagac
      841 ttcgccggct gggaaatgcc tgtgcagtat accggcctcg ctgatgaaca cgacaccgtt
      901 cgcaataaag ttggattgtt cgatgtttct catatgggcg aagttcgagt tcgaggacgt
      961 ggttcgctcg agtttctcaa taaattactc accaacaacg tagcaaaaat tcagttgggt
     1021 caggctcaat acaacgtcat gtgttatgag gatggcgggt gtgtagatga cctcattgtc
     1081 cataaaatgg gcgaagagga ttatttcatc tgcgtaaatg cttcaaacac cgaaaaagac
     1141 ttcgaatgga tgcggcaaca cgctccggcg tctttatctc tcgaaaatgt ttctagcgaa
     1201 tacactcaaa ttgccattca gggtcggttc gcagcggaaa ctctagcccc cctcaccgac
     1261 gcgagtctcg attcgattaa gtactactgg tttcagcaat cttcgatttt gggtaaacct
     1321 gccataattg ccaggactgg ttacaccgga gaagacgggt tcgaagttta tattccctgg
     1381 gaccatggtc ccaaggtgtg gcaaacatta ctcgaaagtg gcgctggccg aggaattaag
     1441 ccgtgcggac taggcgcccg tgataccttg cgtacagaaa tgaagttcgc actttatggt
     1501 cacgaaatcg aaaaagattt aaatcccctc gaagccgggc tcggttgggt ggtaaaactt
     1561 gagaagtcag attttattgg tgccggcgcg ctgcggcgaa ttcaagagac gggccccaaa
     1621 cgcgcgttgg ttggccttaa aagcctagag cgcggaattc cccgacaggg ttataaggta
     1681 gagcacaacg gtcgggaagt tggactggta acctcgggga ctatgtcacc ttcattgcgc
     1741 gtcggaatcg cgattgctta tgtagaacgc gatttatctg ctgtcgggac tacgcttgat
     1801 gttgtaataa gggatcaaaa agtacggcat gaaattgtaa caacgccgtt ctacaaaaga
     1861 ccctattgaa tgcgatagca ggagaatacg ccgatggaat ttccctctgg cctttactac
     1921 acaaaagatc atgaatgggc aaaatgggac gaaaagaccg gaactgtcac agtaggcatt
     1981 accgatcatg ctcaagattc tcttggagac attgtctatt tagagcttcc tcaaaaagga
     2041 cgctccctta aaacgcacga aacatttggc gtggtcgaat cgatcaaggc cgtcagcgat
     2101 ctctattccc ccgtatctgg ggaagtggtc gagactaacg ccaaagctgc agaagatccg
     2161 tcggttctca atcgcgcggc ctacacagat ggctggctta tgcgcttaaa agttgccgac
     2221 tccgctgcac tttccgggct catgagctcc aaagattacg aaatataca
//
LOCUS       DAOMXO010000393         2269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27653_length_2269_cov_5.109756, whole genome shotgun sequence.
ACCESSION   DAOMXO010000393 DAOMXO010000000
VERSION     DAOMXO010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27653_length_2269_cov_5.109756"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLH57_08300"
     CDS             complement(<1..1010)
                     /locus_tag="PLH57_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HRK02653.1"
                     /translation="MKVLVTGVAGFVGFSVAKALLEKDLSIVGIDNLSQYYDPTLKKA
                     RLASLGERKRLQVLIVDISDSAAVLDIFKTERPDIVVHLAAQPGVRYSISHPHSYIQS
                     NIVGFLNILEACRTHPVKHLIYASTSSVYGLNSKLPFSIHDHASHPISLYAATKRSNE
                     LMAHNYSHLFGIPSTGLRFFTVYGPWGRPDMSFFTFTKNILAGEPIEVFNEGRHRRDF
                     TYIDDVVDSILKVIETPPPAPNPDWDRTVADPASSSAAFKIYNIGGNKPVELLKYIQQ
                     IEERVGKKAIKLLKPLQPGDLEVTEADISESKLELGYSPRVPLEEGISRFIHWYRGYY
                     NI"
     gene            1075..>2269
                     /locus_tag="PLH57_08305"
     CDS             1075..>2269
                     /locus_tag="PLH57_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRK02654.1"
                     /translation="MNKDVKFALIGNGRVASRHIEALTKRISGGQLVAVCDSNPSRLE
                     DKRSFGDRVEVFNSSADLYGWGKFDVAAIATPSGDHYEHAKEAILAGKHVVVEKPVAL
                     RIEHANELILLAERNNVKAWVIHQNRYNPAILKTKEAIERGRLGTKVLATVRVRWCRN
                     AEYYTQDNWHGRWASDGGVISQQAIHHLDALRWLFGDVESVEAVCRTRLAPMECDDLT
                     IATMSFKNGALGIVEATTATRPRDLEASLSLLGEQGTIVLGGIAMNTIQTWEFTAPGD
                     GDSDVPQRYSQVIPSAYGLGHDVLYQKVVDSIVDGAPIEVSLDEGKKTLEIVHALYAS
                     NELGRRVKLDEGLSSAKLGVRTLGKNEPGKAHKELQLVQKNISNQLPEFSKNIVNFAG
                     IIGESP"
BASE COUNT          629 a          453 c          574 g          613 t
ORIGIN      
        1 taaatgttgt agtatcctct gtaccaatga atgaatcggc ttatgccttc ttctaatgga
       61 actcggggcg agtaacccag ttcgagtttc gattcggaaa tatccgcctc cgtaacttcg
      121 agatccccgg gttggagtgg ttttaatagc tttatggctt tttttccgac gcgctcttcg
      181 atctgctgta tatatttcaa aagctcgaca ggtttgttgc cgccaatatt atagatttta
      241 aaagcagcgc tactagaagc cggatcggct acggtcctgt cccagtctgg gtttggagct
      301 gggggcggtg tttcaatgac ctttagtatc gaatcaacta cgtcgtcgat ataagtgaaa
      361 tctctacggt ggcggccctc gttaaacact tcgatcggtt ctcccgctag gatattttta
      421 gtaaatgtga agaaagacat gtctggtcta ccccatggac cgtagacagt gaaaaatcga
      481 agaccagtag agggaattcc aaaaaggtga ctatagttat gagccattag ctcattcgag
      541 cgcttagttg cggcatatag tgaaatcggg tgactcgcat gatcatgaat tgagaacgga
      601 agttttgagt taagcccgta gaccgaacta gtagaagcat atattaggtg cttaactgga
      661 tgggttcggc aggcctctaa gatatttaaa aagcctacga tattagactg aatatacgag
      721 tgggggtgag agatcgaata tctaaccccg ggttgcgcag ctaaatggac gacgatatca
      781 ggacgctctg ttttgaaaat atccaacacg gcggctgaat ccgaaatgtc aacgatcaaa
      841 acctgaagtc gtttgcgttc gcccaggcta gccaaacgag ccttcttgag cgtcggatcg
      901 taatattggc tcagattgtc gatcccaaca atgcttagat ctttttcgag aagagctttg
      961 gcaaccgaaa agccaacaaa tcctgcgact ccggtgacga gaactttcat tcggcatatt
     1021 tacgctattt tgatggacat tcaaaattca tttttaatat gaatacatat atttatgaac
     1081 aaagacgtta aatttgccct tattggaaat ggccgcgttg cttctcgtca tatcgaagcg
     1141 cttactaaga gaatcagtgg tgggcaattg gtcgccgttt gtgattccaa cccctcccgc
     1201 ttggaagaca agcgatcctt tggtgatcgt gtcgaagtct tcaattccag tgcggacctt
     1261 tatggttggg gcaaattcga tgttgcggcg attgctactc ccagtggtga tcattacgag
     1321 catgcgaaag aggctatttt agctggaaag catgtcgtag ttgaaaaacc cgtcgcgcta
     1381 cgcattgaac atgcgaatga gctaatttta ctggctgaga gaaacaacgt aaaagcgtgg
     1441 gttattcacc aaaacaggta caatccagcc attttaaaaa cgaaagaagc gatcgagcga
     1501 gggcgattag gtacaaaggt gcttgcaaca gtgcgggtga gatggtgtcg aaacgccgag
     1561 tattatactc aagataattg gcatgggcgt tgggcttcgg atggcggagt tatatcacaa
     1621 caggcgattc accacttaga tgcgttgcgt tggcttttcg gggatgtaga aagcgtcgaa
     1681 gctgtttgtc gaactcggct agctccaatg gagtgtgacg atttaactat agcgacgatg
     1741 agtttcaaaa atggtgcgtt gggaattgtg gaagcgacga ccgccactcg tccgagagat
     1801 ctagaagcgt ctttgtcgtt gttgggcgaa cagggaacga tagtgctagg tggaatcgcc
     1861 atgaacacca ttcagacgtg ggagtttact gcccctggtg atggagattc agatgtcccc
     1921 caacgatatt ctcaggtaat tcccagtgcc tatggtttag gccacgatgt tctttatcaa
     1981 aaggtggtgg attcaattgt tgatggagct ccaattgaag tttctttaga cgagggaaag
     2041 aaaacattag aaattgtgca cgcactttat gcaagcaacg aactgggacg gcgagtaaaa
     2101 ctagatgagg gccttagttc tgcaaaactg ggagttcgaa ctttagggaa aaatgaacct
     2161 ggaaaagccc acaaagaact tcaattagtt cagaaaaata tctcaaatca acttccggaa
     2221 ttttctaaga atattgttaa tttcgctgga attatagggg aatctccgt
//
LOCUS       DAOMXO010000394         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27720_length_2265_cov_4.823982, whole genome shotgun sequence.
ACCESSION   DAOMXO010000394 DAOMXO010000000
VERSION     DAOMXO010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27720_length_2265_cov_4.823982"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1754)
                     /locus_tag="PLH57_08310"
     CDS             complement(162..1754)
                     /locus_tag="PLH57_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRK02655.1"
                     /translation="MSKKKTSTGLSNGIKVFLEWVERTGNKLPDPVFLFIYLTVALVL
                     VSVVCALLGLSAIHPTQKSPDGGAFVIRAASLLSPANIQKFWVDMPATFTHFHPLGYV
                     LVVMLGAGVAERSGLFATAMRFAVSKAPVALLTPLVALIAMISSHAADAGYVVLIPLA
                     GILFAAAGRHPLAGIGAAFAGVSGGFSANITPGQLDALLLGITETAVVASKLDPKWTV
                     NLAGNWWFIIGMTLVFLPTIWFVTDRIIEPRLGKWTPSDSSPVANSEKKNISTAERRG
                     LGRAGWALLGVIGLWVFMVVGPGAPLLGKGAPDAAWYVKAAPLFKSLVSAFFLLFLAS
                     GWAYGAAAGTIKNHRDLVKMMADSMKDMGYYLVLAFVAAHFVAMFGWSNLGLIFAIHG
                     ANGIESTGLPMPALLVLILLFSSALNILIGSASAKWALMAPVLVPMLMLLGVSPEATT
                     AVYRVGDGATNIITPLMVYFPLVLVFAQKWRPDFGLGSLTALMLPYSVAILLVGTLLV
                     LLWVLVGAELGPNAPLHYILPR"
     gene            1901..>2265
                     /locus_tag="PLH57_08315"
     CDS             1901..>2265
                     /locus_tag="PLH57_08315"
                     /inference="COORDINATES: protein motif:HMM:NF018604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HRK02656.1"
                     /translation="MFNHRFDETPNNRVVFHWFLLSFSGGCINSGGFLATGRFVSHVT
                     GFATLFGVDIMTHQIEAALGILSVPLFFLLGAFIAGLMIDRPIYHGNKPHFDWVMGLS
                     ALCLFLAASGGELLHFGIFG"
BASE COUNT          590 a          619 c          537 g          519 t
ORIGIN      
        1 gttcggtttt ttttgggcgc tcttcggttc acgctcaacg tcctactcat gcgccacaaa
       61 attaagctcg caagacagac cgtcccccgg ttttttgtgt cggatgccgt tttaatgcag
      121 cacaagtagc cgatggagta cggtagcaat ttcgctacgc actacctcgg caaaatgtag
      181 tgcaatggcg cgttaggtcc cagttccgca ccaacgagta cccacagcag taccagcaag
      241 gtcccaacta acaggatggc taccgaatac ggcaacatga gcgcggttag gcttccgagc
      301 ccaaaatccg gtcgccattt ttgggcaaac accaatacca atgggaaata caccatgagc
      361 ggagtaatga tattcgtagc gccatcgcct acccgataca cggccgtcgt agcctcggga
      421 ctcacaccaa gaagcatgag cataggcact aatactggcg ccatcagcgc ccactttgcg
      481 ctcgcggaac caattaaaat attgagtgca gaactgaaca aaagaatgag cacgagcaag
      541 gctggcattg gaagcccggt gctttctatt ccgttggccc catgaattgc gaaaatcaaa
      601 ccaagattcg accagccaaa catggcaacg aaatgtgccg ccacaaacgc gagtaccaaa
      661 taatagccca tgtctttcat tgaatcggcc atcatcttaa ctaggtcgcg atgatttttt
      721 atcgtgcctg cagctgcacc gtaagcccaa ccagaggcga gaaacaataa aaagaacgcc
      781 gaaacgagcg acttaaataa gggtgcggct tttacgtacc acgcagcatc aggcgcccct
      841 tttccaagca agggtgctcc aggtcctacc accatgaaga cccacaaacc tataacaccg
      901 agcagagccc aaccagctcg ccccagacct ctgcgttcgg cagtggagat attctttttt
      961 tcggaatttg cgactgggct tgaatcgctc ggcgtccatt tcccaagccg cggttcaata
     1021 attcgatcgg tgacaaacca aatcgtcggc aaaaagacga gcgtcattcc gatgataaac
     1081 caccaattcc ctgcaagatt cacggtccac ttagggtcaa gtttacttgc caccaccgct
     1141 gtttccgtga ttccgagcaa aagtgcgtca agctgtccgg gcgtaatgtt ggccgaaaac
     1201 ccgcccgata cgcccgcgaa tgcggcacct attcccgcaa gagggtgccg ccccgcggcc
     1261 gcgaataaaa ttccagccaa gggaatcaac accacgtaac ctgcatcagc ggcgtggctg
     1321 gaaatcatag cgatcagggc caccagtggc gtcaaaagcg ccacgggcgc cttactgacc
     1381 gcaaaccgca ttgccgtcgc gaagagtcct gaacgttccg ccacacccgc acctagcatg
     1441 acgactaata cgtagccgag cgggtgaaag tgcgtaaaag tggccggcat atcaacccaa
     1501 aatttttgaa tattagcagg cgacaacaaa ctcgccgccc gaatgacgaa ggccccgccg
     1561 tctggacttt tttgggtagg gtgaatagcg gaaagtccta aaagcgcaca aactactgaa
     1621 accaatacca acgccaccgt taagtagatg aataagaaca ccggatccgg gagcttgttt
     1681 ccagtgcgct caacccattc gagaaagacc ttgattccgt tcgataagcc ggttgaagtt
     1741 ttctttttac tcattcggta tgtaccccta ccggggggtt actacagcga cgcgcatttc
     1801 gcaatcctcc atcgccggtg cacttagaac tttgaacgct atcatttcaa tcgactgtcg
     1861 gtcttataaa acttaaagat tagtggtaca aaggctatta atgtttaacc atcgtttcga
     1921 cgaaacccca aacaatcgcg tagtattcca ttggtttttg ctaagtttta gcggcggctg
     1981 tatcaattcc gggggttttt tggccacggg gcgattcgtt agtcacgtta caggtttcgc
     2041 caccttgttt ggagtcgaca ttatgactca tcaaattgaa gccgcactgg gcattctttc
     2101 agttccactt ttctttctgc ttggggcctt tatagcgggc cttatgattg atcgaccgat
     2161 ctaccatggc aataagcctc actttgactg ggttatgggt ctcagcgcgc tttgtttgtt
     2221 tttagcagca agcggcggag agttgctaca tttcggaatt ttcgg
//
LOCUS       DAOMXO010000395         2263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27761_length_2263_cov_4.319746, whole genome shotgun sequence.
ACCESSION   DAOMXO010000395 DAOMXO010000000
VERSION     DAOMXO010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27761_length_2263_cov_4.319746"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLH57_08320"
     CDS             complement(<1..842)
                     /locus_tag="PLH57_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02657.1"
                     /translation="MFNRVLVMFLVLMVFAASGTRAASPIDCNFALVPLTPDQIANRV
                     DVSFERARKKAPRFQRFFLKIKSKRLRKNIISACERRADCTVLHVDHEVAKGFRSYKE
                     KMQRLNGFGKVLLIFGGAMVLPNLAVYLMPDDLVAQYGTAVRYGGMALVGALGAPFLS
                     TVKSETWEWMSGITANGEFDLTTPEVAKRLKDGWRRTQDNYGPNDLSHATRLILYLMY
                     IESTLPDVAKRLRSRTAPERERAVADFAGKLMVVRELAVDLDQKNLLVQETVRSYLKF
                     RDQEA"
     gene            complement(920..2182)
                     /locus_tag="PLH57_08325"
     CDS             complement(920..2182)
                     /locus_tag="PLH57_08325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRK02658.1"
                     /translation="MTETQENPSVEDESSETAESAESAESNDSSDSQEAGSENNESSA
                     AVGAPPASEVGPAWEDLGLSSEVLELVRKEGYTAPTPVQGQAIPALLAGRDLIVSAQT
                     GTGKTAAFVLPLVEKLKGRSGTLGLILAPSREIALQTQAVMEVFGTPLGVRSVALIGG
                     IDLKIDAEALRTYPQVIVATPGRLCDHADRGNVWLEYLEFLILDEADRMLDMGFSAQL
                     NRILEETPKSRQSLLFSATFPPPVEKLANAMLTDPQRIQIGKSVSAATTVEQRFLFMP
                     EKAKLNQLQRLLEREEGTVFIFTRSKDGASRLWRSLRGRGFENVTQLHSDLRQADREQ
                     ALEDFKNGVYRIMIATDVIGRGIHVEGVAHVVNYDLPRDAEDYVHRVGRTGRAKARGR
                     ATSFITPGDYFTLAKIERLLGRRIRPRG"
BASE COUNT          554 a          621 c          527 g          561 t
ORIGIN      
        1 gcctcttgat cgcgaaattt taaataggag cgaacggttt cctgaacaag taaatttttt
       61 tgatctagat cgaccgcaag ctcacgcact accatgagtt tgccagcaaa gtcggcaact
      121 gcacgttcgc gctcaggcgc cgtgcggctt ctgagtcgtt tcgctacgtc gggcagcgta
      181 ctttcgatat acattaggta cagaataagc cgagtcgcat gcgacaaatc atttggacca
      241 taattgtctt gagttcgacg ccaaccatct ttaagtcgct ttgcgacttc tggagttgtg
      301 aggtcaaact caccgttggc ggtgatcccc gacatccact cccaagtctc ggactttaca
      361 gtagacaaaa atggggcacc caacgctccc accaacgcca tgccaccata tcggactgcg
      421 gttccgtatt gcgcgaccag gtcatcgggc atcaggtaga ctgccaagtt gggcaaaacc
      481 atcgctcccc cgaaaatgag aagcactttt ccgaatccat ttaatcgctg cattttttct
      541 ttataacttc tgaacccctt cgccacttcg tgatctacat gcaagaccgt acagtccgct
      601 ctacgttcac aagcagaaat tatatttttt cgaagccgct ttgattttat tttaagaaaa
      661 aatcgctgaa aacgcggtgc ctttttccgc gctctttcaa aactaacgtc gacacggtta
      721 gcaatctgat ccggcgtcaa tggaactaaa gcaaaattgc agtctatagg actagccgct
      781 cgcgtgccag acgccgcaaa caccatgagt accaagaaca ttaccaacac gcgattaaac
      841 acgatgacac cccaatctct aagtctcgca gagttacagt tggggttgtc ctagcattcg
      901 atggccgagg ccgccagaat taaccgcgcg gacgaattcg acgcccgagc agacgctcga
      961 ttttcgctaa agtaaaataa tcgcccggtg taatgaaact agttgcccga cctcgagcct
     1021 tggctcggcc cgtgcgacca actcgatgca cgtaatcttc ggcgtcgcga ggaaggtcgt
     1081 aattcacaac gtgagcgacc ccttcgacgt gaatgccgcg gccgatcaca tcggtggcga
     1141 tcatgattcg atatacaccg tttttaaagt cctcaagcgc ttgttcgcga tcggcttgtc
     1201 gtagatctga atgcagttgc gtaacgtttt caaagccgcg tccacgtagc gaacgccaca
     1261 accgtgaagc tccatctttg gagcgcgtga aaataaaaac tgtgccttct tcacgttcca
     1321 ataagcgttg caactgattg agtttcgctt tttcgggcat aaacaaaaat cgttgctcca
     1381 ctgtagttgc cgccgaaaca gattttccaa tttgaattcg ctgcggatcc gtgagcatgg
     1441 cattggcaag tttttctact ggcggaggaa acgttgcaga aaacaaaagc gattgtcgcg
     1501 atttcggcgt ttcttcgaga atgcgattta gttgcgccga aaatcccatg tctaacatgc
     1561 gatctgcttc gtcgagaatt agaaactcta aatattccaa ccacacatta ccgcggtcgg
     1621 cgtgatcaca cagacgcccg ggtgtggcca caatgacctg ggggtaagtg cggagtgctt
     1681 cagcgtcgat cttgaggtcg attccaccaa tcaatgccac cgaacgcacg ccgagtggcg
     1741 tgccgaacac ttccattacc gcctgagtct gcagagcaat ttcacggctt ggcgccagta
     1801 ttaacccgag ggtgccggag cggcctttta atttttcaac caacggcaac acaaacgccg
     1861 ctgttttgcc ggtaccggtc tgagccgaaa caatcaaatc tctaccggcg agcagagccg
     1921 gaatggcttg gccctgcact ggcgtcggag ccgtgtaacc ttccttgcgg acaagctcta
     1981 aaacctcaga actcaaaccc agatcttccc aggccggccc cacttccgaa gcgggaggag
     2041 cgcccaccgc agcactggac tcgttgtttt cggaccctgc ttcctggcta tcgctggaat
     2101 cgttggattc ggcggattcg gcggattcag ctgtttccga agattcgtct tcaacgctcg
     2161 gattttcttg agtttctgtc attttttcgt cttgcaaaat ctattaccaa actttctgtt
     2221 ttgatatcct tataccctaa attgaccgat cccgacactg cga
//
LOCUS       DAOMXO010000396         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_27951_length_2251_cov_4.950820, whole genome shotgun sequence.
ACCESSION   DAOMXO010000396 DAOMXO010000000
VERSION     DAOMXO010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27951_length_2251_cov_4.950820"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLH57_08330"
     CDS             <1..260
                     /locus_tag="PLH57_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HRK02659.1"
                     /translation="MNKNKKKYPSITNVGVKPTFAKPEDQRPPVVIETHLFDFNDDLY
                     GRTIEIELVERIRDEQKFDSIEKLVEQIRKDVAQTRQLFTI"
     gene            351..2060
                     /gene="pilB"
                     /locus_tag="PLH57_08335"
     CDS             351..2060
                     /gene="pilB"
                     /locus_tag="PLH57_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HRK02660.1"
                     /translation="MSRALADILLRDKIITQQQHQEAVDANKARGESHVRYLIAKKYV
                     AEPKLLQFLSQKYSMPAINLAKYEINQEIIKIVPMDLAKKHNVVPIQTNKNVLVVAVA
                     DPTVLVNIEDIKFRTKMNIEAVLTTPSALDATLNRYYSGSVLMADAVSQFQKTQEKDN
                     ENAFEGLNASAIQIDAGAMTDKDAPVVSLVNNILVEAMRKGASDIHIESYEHRSRVRL
                     RIDGVLQEVIQIPPEMKRPLMARVKIMSKLDISESRMPQDGRIKIKVGTGEIDFRVST
                     MPCLFGEKAVLRLLSKGNLQLDLGKLGFEPKQLEIFRKGTYQPNGMVLVTGPTGSGKT
                     TTLYSALAELNKSTDNISTAEDPVEYNLDGINQCQVNKDINLTFASVLKTFLRQDPDI
                     ILVGEIRDAETAEVSIQAALTGHLVLSTLHTNDAPSTVMRLMNMGIEAFLVTASVNTI
                     VAQRLLRSVCPKCREPMQVPIARLVELGIAEEIATQLKLFKGRGCPTCNNTGYKGRTA
                     VYEVLDFTPTLKEMILAGATALEFKRAAIREGMKTLRMSALQRAAEGKTTLEEALSIT
                     AAE"
     gene            2064..>2251
                     /locus_tag="PLH57_08340"
     CDS             2064..>2251
                     /locus_tag="PLH57_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HRK02661.1"
                     /translation="MAKVPLPALLKAMVDQNASDLHVTVGSPPQFRIEGRMVRIKMES
                     LGPQETKELCYSVLTDSQ"
BASE COUNT          709 a          488 c          511 g          543 t
ORIGIN      
        1 cgatgaataa aaacaaaaaa aaatatccat cgattacgaa cgtaggggta aaaccaactt
       61 ttgctaaacc agaagaccag cggcccccag tcgtgattga gactcatctt tttgatttta
      121 acgatgatct ttatggccga actattgaaa ttgagctggt cgaacgaatc agagacgagc
      181 agaagtttga ttcgattgaa aagcttgtcg aacaaattcg aaaagatgta gcgcagacgc
      241 ggcaactttt taccatttaa gtgcaatctt tgccgggagt agcaaacttt accggctgtc
      301 gaaaaatgtc actgttcgtt agcattggat gaagaaggat aaagaatcca atgtctcgag
      361 cactagcaga tattctgctg cgtgacaaaa tcatcacgca gcaacaacac caggaagcag
      421 tcgatgccaa caaggcacgc ggcgagtctc acgtacgtta tttgatagcc aaaaagtacg
      481 tggctgagcc gaaacttttg caatttctaa gtcagaaata cagcatgccc gcaattaatt
      541 tggcaaagta tgaaatcaat caggaaatta taaaaattgt gccaatggat ttggccaaaa
      601 aacacaatgt agtgccgatt caaacgaata aaaacgtcct agtggtagcg gtcgcagacc
      661 cgacggtact agtaaatatc gaagacatta agtttcgcac taaaatgaat attgaagcgg
      721 tgttaaccac accgagcgcc ttagatgcca cattaaatcg ctactattcc ggcagtgttc
      781 tgatggcaga tgcagtgtct caatttcaaa aaactcaaga aaaagataac gaaaatgcat
      841 tcgaaggctt gaatgcctcg gccatccaaa tcgatgccgg agcaatgacc gataaagacg
      901 ccccagtcgt aagcttagtg aacaatatac tcgtagaggc aatgcgaaag ggggccagcg
      961 atatacatat agaatcttac gaacatcggt ctcgcgttcg attgcgaata gacggtgtcc
     1021 tgcaagaagt aattcagatt ccaccagaaa tgaagcgacc gctcatggcg cgtgtaaaaa
     1081 ttatgagcaa acttgatatc tcagagtcgc gcatgccaca ggatgggcgt ataaaaatta
     1141 aagtcggtac tggtgaaatc gactttcgtg tatcaacgat gccatgtttg ttcggtgaga
     1201 aggccgtttt acgtctactg tcaaaaggca acttgcagtt agatcttgga aaattaggtt
     1261 tcgaacctaa acaactagaa atctttcgca agggcaccta tcaaccaaat ggaatggtac
     1321 tagtcaccgg acccaccggt agcggcaaaa ccacgacact ttattctgcc ttagcagagt
     1381 tgaataaatc gaccgataat atttccacag ccgaagatcc tgtcgagtat aatctcgatg
     1441 gaatcaatca atgtcaggtc aataaagaca tcaacctgac ttttgcttct gttcttaaaa
     1501 cgtttttgcg ccaagatcct gatattattc tagtcggaga gattcgagac gccgaaactg
     1561 ccgaagtctc aattcaagct gcgctaactg gccacttagt attgtccact cttcatacga
     1621 atgacgctcc gagtaccgtg atgcgattaa tgaacatggg aattgaggcg tttctggtga
     1681 ctgcatcggt aaatacaatt gtggcacaac ggcttctcag gtcggtatgt cccaagtgtc
     1741 gcgagccgat gcaagttccg atagctcgcc ttgttgaact tggaatcgcg gaagagattg
     1801 ctactcaact aaagttattt aaaggtcgtg gttgccccac ctgtaacaat accggctata
     1861 agggtcgtac cgcagtctac gaggtgttgg acttcacccc taccctaaaa gaaatgattt
     1921 tagctggtgc tacagcgtta gaattcaagc gagcagcgat tcgcgaagga atgaagacgt
     1981 tgagaatgtc ggcgctccag cgcgctgcag aaggcaaaac aactttggag gaggccctgt
     2041 ctataacagc ggcagaataa tttatggcaa aagtccctct tcccgcactg ctgaaagcaa
     2101 tggtcgatca aaacgcctca gatttacatg taactgttgg gtcccctccg caatttcgca
     2161 ttgagggacg aatggtacgt ataaaaatgg agagccttgg accacaagaa acaaaagaac
     2221 tttgttacag tgtattaacc gactctcaaa a
//
LOCUS       DAOMXO010000397         2236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28247_length_2236_cov_4.324163, whole genome shotgun sequence.
ACCESSION   DAOMXO010000397 DAOMXO010000000
VERSION     DAOMXO010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28247_length_2236_cov_4.324163"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PLH57_08345"
     CDS             complement(<1..1143)
                     /locus_tag="PLH57_08345"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HRK02662.1"
                     /translation="MQRSLTFLLGLALLVGCSMEVPKWSGSGLRIILEEQPQNTLITN
                     LHNTFNTFAPTIDAPTSISDFNCFAVNVTGEGIQSNPDALSDCSSTDNFAGVGVGIVS
                     KGLFSRGQSITLDIPAGPKRNIDVYGIYPPANDCPGGASGASGSSSDSGYFLGRATRD
                     LSEPATVVIPISYSGASATITCTEPTPNSSGSSTSMAVGTRDTSFNNGGAGFSTPAVN
                     FILPELNTGTGNGTGSYLIVNGNSTYNGNTVPNKLVRIFSNGTADTSFNNGGAGFDGP
                     AYAALQQVYAGSLSGSFIVGGSFTTYNGNIVPDGLMKIDSSGNHDTTFNNSGVGFDGA
                     VYTLAQYPPSETLIAGGSFTTYNGTSANRIVRLNNSGTPDATFLTNM"
     gene            1250..1609
                     /locus_tag="PLH57_08350"
     CDS             1250..1609
                     /locus_tag="PLH57_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRK02663.1"
                     /translation="MLKLLRLFVPALLPIVSIAAPPPSTPASVAKGKQIFSIHCIVCH
                     GENMDGQGPAGKALDPRPRNLISDKLKKGETRDELFKTISEGLAGTAMIAYGHLPTEE
                     RWALVDFILSKRKAKNK"
     gene            1621..>2236
                     /locus_tag="PLH57_08355"
     CDS             1621..>2236
                     /locus_tag="PLH57_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02664.1"
                     /translation="MTLNVKFESKRKRCSLRGALYFVAVISLVFCHTLHATNPKPPKD
                     RTVLRKCVTILKQYSDTEIKKMGPLLLGTALSRSLVPTNTEKSGDNTVKLVVQITGSL
                     SPRHGEASALSLSDLWEVEQVFVGLVSAMIVNVPIKNLPAFLAHDSVLAAEIEQANVN
                     LNLFSTADENLLRELLHSDSKVVLDLRVIGSAREFEESERLAAAY"
BASE COUNT          598 a          507 c          548 g          583 t
ORIGIN      
        1 catatttgtt aaaaaggtgg catcgggagt acctgaattg tttaagcgca cgattcgatt
       61 agcgctcgtt ccgttatagg tcgtaaagct accgcccgcg atgagagttt cgctgggggg
      121 gtactgagca agtgtataaa cggcgccgtc gaatccaacc ccactattgt taaacgtagt
      181 gtcgtggttg cccgaactgt caattttcat caaaccatcg ggaacgatgt taccgttgta
      241 agttgtaaag ctgccaccca ctataaatga accactgagc gacccggcat atacttgctg
      301 aagcgctgca taggccgggc cgtcaaaacc cgctcctccg ttattgaacg atgtatctgc
      361 agtaccgtta ctaaatattc gcaccaattt attaggcacc gtatttccgt tgtaagtact
      421 atttccgttc acgattaaat aagacccagt gccgttgccg gtgccggtat ttaattcggg
      481 caatatgaag ttaacagctg gggtcgaaaa tcctgcgcca ccattgttaa acgaagtgtc
      541 gcgagtacca accgccattg aggtagacga cccagaagag ttcggggtgg gttcagtgca
      601 agtaattgtt gctgaagcgc cagaatagga aatgggaata accacagtcg ctggttcaga
      661 aagatctcga gtcgctctac ccaaaaaata gccactgtcg ctactagacc ccgaagcacc
      721 cgaagccccg ccggggcagt cgtttgccgg cggataaatt ccgtaaacgt cgatgtttct
      781 tttggggccc gcgggaatgt cgagggtaat actttgacct cgcgaaaaaa gcccttttga
      841 aacgatgcca actcccacgc ctgcaaagtt gtccgtcgaa gaacagtccg acaaagcgtc
      901 cggattggat tgaatgcctt cgccagttac atttactgcg aaacaattaa aatcgcttat
      961 cgacgtaggt gcgtcgatag tcggtgcaaa ggtattgaag gtattgtgaa ggtttgtaat
     1021 cagtgtgttt tgcggttgct cttcgagaat aatgcgtaag cccgaaccgg accacttagg
     1081 tacttccatt gaacaaccta ccaatagtgc cagccccaat aaaaacgtaa gtgatctttg
     1141 cattcgacaa tcgtgctatt tgattggtgc tttttcaaga ctaagtaaaa ccaatgtttt
     1201 taattcacaa actatgtgcg tcggcgttat ggctcggttt ggaggattta tgttgaagtt
     1261 acttcgtttg ttcgtacctg ctctgttacc aatagtatcg attgcggctc cccctccttc
     1321 gacgccggcc agcgttgcca aaggcaaaca gattttctct attcactgta tcgtgtgtca
     1381 cggtgaaaac atggatggcc aaggtccggc gggaaaagcg ctcgatccgc gccctagaaa
     1441 cttaatttcg gacaaattga agaaaggtga gacccgcgac gaacttttca aaacaatatc
     1501 tgagggatta gcgggaaccg caatgatcgc ctatggccat ttaccaaccg aagagcgctg
     1561 ggcgttggtc gattttattc tctcaaagag aaaagcaaaa aataaataaa ttagaactaa
     1621 atgacgctca atgtgaaatt tgaatccaag cgaaaaaggt gtagcctccg aggggcgctt
     1681 tattttgtcg cagtcatctc actggttttt tgtcacacac tgcacgctac gaatccaaaa
     1741 ccgccaaaag atcgaacggt tttacgtaaa tgtgtaacca ttttgaaaca atattccgat
     1801 accgagatta agaaaatggg gcctctgttg ttgggcacgg ccctgtcgcg gagtctggtt
     1861 ccaacaaaca ccgaaaaaag tggcgacaac acggttaaac tagtggtgca gattacgggt
     1921 tcactttctc cccgtcacgg cgaggctagt gcgctttctc ttagtgatct ttgggaggtg
     1981 gagcaggtgt tcgttgggct agtttcagcc atgatcgtaa atgttcccat taaaaatctt
     2041 cccgcttttt tggcgcacga tagtgttttg gcagcagaga tcgaacaggc caatgtgaat
     2101 ttaaatcttt tttccacagc cgatgaaaat ctattgagag agctgcttca tagcgattcg
     2161 aaggtggttc ttgatctaag ggtgatcgga tcggccaggg aatttgaaga gtccgaaaga
     2221 cttgctgcgg catact
//
LOCUS       DAOMXO010000398         2234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28282_length_2234_cov_4.656264, whole genome shotgun sequence.
ACCESSION   DAOMXO010000398 DAOMXO010000000
VERSION     DAOMXO010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28282_length_2234_cov_4.656264"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLH57_08360"
     CDS             <1..527
                     /locus_tag="PLH57_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02665.1"
                     /translation="NRHELRLLFAPLQFEIKGQFRSPVKYIGSDFAAETETTAFYKFN
                     SYRLTYAYHFDPISQWELALGFTAKVRDAEVRLTQGNKLERKTNIGFVPLLHFQARRP
                     LFADLFFRFDFDGLAAPQGRAFDAALFLERSFIAPAYTFFLGYRTVEGGANNDTVYNF
                     AWFHYALGGVRASF"
     gene            complement(546..>2234)
                     /locus_tag="PLH57_08365"
     CDS             complement(546..>2234)
                     /locus_tag="PLH57_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRK02666.1"
                     /translation="GNDITSSVLYVSALTIGVAGIYAPIGLLLVSLVLYGFRKVYGEV
                     GAALPLNGGAYTVLLNTTNKKVAAGAATLTIISYLATAVISATEAMHYLHSILPSIDP
                     APATVALLGAFALLSIVGITESSKVAVVIFIVHIVSLTMLVLTCVALVFTDGFAFSLD
                     RMFESGRQVSPLTGIFVGFCAGMLGISGFESSANFIEEQKPGVFPKTLRNMWAAITVF
                     NPLICVLTLLVIGAASTQEYENSLLSELARRSGGPWLQRLIGIDAFLVLSGAVLTSYV
                     GVIGLVRRMTLDRCLPQFFLKENKWRNTNHWIILGFFLLSCAILVLTGGKVSALAGVY
                     ALSFLSVMALFALGNLFLKKTRARLPRSEEAKLSVVAVCLFAVLVAGVYHVTVENIRS
                     LLVFVTILGGAVAIMFSRILILRLGLFSLKSVVDSFAGASHHFQKFLYNAIENANSQA
                     VIYFSRGDDLKILNHAALYVLRNEQTNNLIVINVYRNENEIPPNLANQLRVIDQLYPE
                     IRIDFLAVQGAFGPQVVEKLSKRLGIPKNCMFIGTPGTNFKHSLAELGGVRIIL"
BASE COUNT          637 a          601 c          492 g          504 t
ORIGIN      
        1 ataaccgtca cgaactgcga ctactctttg cacctttgca gtttgaaatt aaaggtcagt
       61 tccgtagccc tgttaaatac attggcagcg acttcgccgc cgaaaccgaa accactgcgt
      121 tttacaaatt caattcctac cgcctgacct acgcctacca ctttgatccg atcagccagt
      181 gggaacttgc gctcggcttc accgccaagg tgcgtgacgc ggaagtacgc ctaacccagg
      241 gcaacaaact cgagcgcaaa acaaacattg ggttcgttcc actgttgcac tttcaagctc
      301 gaaggcccct ttttgctgac ttattttttc gttttgattt cgatgggctc gcggccccac
      361 agggtcgcgc gtttgacgcc gcactttttc tagagcgtag ttttattgct ccggcttaca
      421 cttttttcct gggataccgc accgtcgaag gcggcgccaa taacgacacg gtctataatt
      481 ttgcgtggtt tcactacgcg ctcggcggcg taagagcatc attctaactt tttatagttt
      541 ccgccctaca aaataatgcg cacaccaccg agctccgcca gcgaatgttt gaaattagta
      601 cccggcgtcc caatgaacat gcagtttttt ggaattccca aacgcttcga cagtttctct
      661 acgacctgcg ggccaaacgc tccctgtact gccagaaaat ctattcgaat ctcaggataa
      721 agttgatcaa tcacccgaag ttggtttgcg agattagggg gaatttcatt ttcgtttcga
      781 taaacgttaa tcacgatcag attgttagtt tgttcatttc gcaaaacgta aagggccgcg
      841 tgattaagaa tttttaagtc gtcaccccgt gaaaaataaa taaccgcctg actattggca
      901 ttttcaatag cgttataaag aaatttttga aaatggtggc tagctccggc aaatgaatcc
      961 accacacttt ttagtgaaaa cagcccaaga cgcagaatta aaatccgcga aaacatgatc
     1021 gcgaccgcgc caccaagaat agtaacaaac accaagaggc tacgtatgtt ttcgacggtg
     1081 acgtggtaaa caccggccac caagaccgca aataaacaca cggccaccac cgagagtttg
     1141 gcttcctcac ttcgaggcag tctggcccga gttttcttga ggaacaagtt tccaagtgca
     1201 aacaaagcca tgaccgacaa aaacgagagc gcgtaaactc ccgccaaggc cgacacctta
     1261 cccccggtta gaaccaaaat cgcacacgaa agtaggaaga accccagaat gatccagtga
     1321 ttggtgtttc tccacttgtt ttctttcaag aaaaactggg gcaaacagcg atccaaggtc
     1381 atgcggcgta cgagtccaat aacaccaaca taagaagtaa gaactgcacc cgaaagcacc
     1441 aaaaacgcgt ctattcctat cagacgctgc agccaaggcc ccccgcttct gcgtgctagt
     1501 tccgacagca aagaattctc gtactcctgg gtagatgccg cgccaatcac caaaagcgtt
     1561 aaaacacaga ttagtggatt aaaaactgta atcgcagccc acatgttacg aagagttttc
     1621 ggaaatactc cgggtttttg ttcttcgata aagttagcgg aactttcaaa tccagaaatt
     1681 cccaacatgc ctgcgcaaaa ccccacaaaa ataccagtca gtggcgacac ctggcgcccg
     1741 gactcgaaca ttctatcgag ggaaaacgcg aacccatcgg tgaacactaa ggctacgcac
     1801 gtaagcacca gcatggtgag cgaaacgatg tgaactataa aaataaccac tgcgaccttg
     1861 gaagattccg taatacccac gatcgagagc aacgcaaacg cccccaatag tgcgaccgta
     1921 gccggagccg ggtcaatact tggcaaaatc gaatgaagat aatgcatcgc ctcagtagcg
     1981 ctaatcaccg cggtcgcgag gtaagaaatg atagttaatg ttgcggcgcc ggcggctact
     2041 tttttgttcg tcgtgtttag caaaacggta tacgccccgc cattgagcgg aagtgccgcg
     2101 ccgacctcac cgtacacctt gcgaaacccg tagagcacga ggctcaccag caaaagaccg
     2161 atcggagcat agattccggc cactccaatg gtgagcgccg acacataaag cacacttgaa
     2221 gtaatgtcgt tacc
//
LOCUS       DAOMXO010000399         2234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28286_length_2234_cov_3.515833, whole genome shotgun sequence.
ACCESSION   DAOMXO010000399 DAOMXO010000000
VERSION     DAOMXO010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28286_length_2234_cov_3.515833"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..1994
                     /locus_tag="PLH57_08370"
     CDS             318..1994
                     /locus_tag="PLH57_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02667.1"
                     /translation="MRQFLSARKSRPEALTKAQSHTTSAVIRGITIVLALSFGVQTAL
                     ARGTRKTKTPPNNVSVVALPEITPAPKVATPKPEAEAKYKKTKYDEELLDKHSVIIIE
                     RHAEPSNATEYTQEEFAKMVANKELPRLEYHFDLRAIYEDRDALAVAMGLMEDDGSVK
                     FKKCKSFRKNRDGEMVPCGYFYTAWRIEPGKEPVSLAGKRGGYSFDEFSQALGVGAKN
                     IDDYYNKEYGISRTNLKWAVGGTLGALGAGAWIWRYKAWARKIDAWREAGKTDKQLWA
                     GARLMQGWASWFVGHDRKVLGKTAFGRGAKKVGFFIKDKMMFTIGAHMYICTFTGHNA
                     ASNVTSNSIASYQCGLLGMPWVPSFGEMILGGLFKNDDGIAKVGGWLDNRATRAIGEM
                     GFITGKMAETQCLLGALLPKESDAAFSCGNDAALKANEKFSMKAALAESSQELEDIEL
                     EEDDADIEAFEAYEKRMDEERERMRKKIEQEKWLGQQQLAIATVMADVIDPQHAERGI
                     DFEQKAVKRFFSIKFSKNPKKGIVTTPGTRYQPKYEDIRAKAESDEEQRP"
     gene            1994..>2234
                     /locus_tag="PLH57_08375"
     CDS             1994..>2234
                     /locus_tag="PLH57_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02668.1"
                     /translation="MQKNRYFMNMKTPLLRILGVQILVALSVVVMTALERPAFSADQL
                     VKDKLEALVGISQKTSREFYRLLSAELRKDQEKSGS"
BASE COUNT          621 a          493 c          606 g          514 t
ORIGIN      
        1 caaacggcta agggtgtaaa tccagccatt gctaagctcg tagggatctt ataagtcccg
       61 cccaattagg ggtttagggg aatattgtta aatcacaata cctcttttgt tgttatgcaa
      121 agaccctgga gagcgaaagc tctctggggt tttttttcgt ccataggagt acccgagagg
      181 tggtctcggg tttgcatttt taccgggaaa ggccgggtgt acccagattc tagggcgtga
      241 gccctagatg gagctgtcta acaaattgac agttgcacca ggtgtttcca tgtgacttga
      301 aaggttttgg cgtttttatg agacagtttt taagtgctcg caagtcccga ccggaagccc
      361 ttacaaaagc ccaatctcac acaacctctg ccgtgatccg tggaatcaca atcgttctgg
      421 ccctgtcgtt tggcgtacaa accgcgctcg ctcgtggcac ccgaaaaacc aagacccctc
      481 ctaataacgt ttcggtagtc gcgcttcctg agattacgcc ggcgccaaag gttgccaccc
      541 caaagcctga agctgaagct aaatacaaga aaaccaaata cgacgaagag ctactcgata
      601 aacattcggt gattatcatt gagcggcatg ccgagccgag caatgcgacc gaatacaccc
      661 aggaagagtt cgcaaaaatg gtagcgaaca aagaactgcc tcgtctggaa taccacttcg
      721 acctccgcgc aatttacgaa gaccgtgatg cgctggccgt ggctatgggt ctgatggaag
      781 acgatggttc ggttaaattt aaaaaatgta aaagctttcg caaaaaccgc gatggcgaaa
      841 tggtaccgtg cggatatttc tataccgcat ggcgcattga gcccggtaaa gaaccggtga
      901 gtctcgccgg caagcgcggc ggttattcgt tcgacgagtt ttcccaggct ttgggcgtgg
      961 gagctaaaaa tatcgacgat tactacaaca aggaatacgg catcagtcgt acaaacttga
     1021 agtgggcagt cggcggcact ctgggagcct taggagctgg tgcgtggatt tggcgctaca
     1081 aagcttgggc tcgtaagatc gacgcttgga gggaagctgg caaaactgac aaacagctct
     1141 gggccggtgc gagattgatg cagggttggg cttcgtggtt tgtgggtcat gatcgaaaag
     1201 tactaggcaa aacggccttt ggtcgcggtg ctaagaaggt tggctttttt ataaaagaca
     1261 agatgatgtt tacgatcggt gctcacatgt acatttgtac ttttaccggt cacaatgcgg
     1321 cgtcgaacgt gacttcaaat agcattgcat cttatcagtg cggcttgctc ggaatgcctt
     1381 gggtcccgtc gtttggtgaa atgattttgg gcggtctctt taaaaacgat gacggcatcg
     1441 caaaggtagg cggttggctt gataatcgag ctactcgtgc tatcggcgaa atgggtttta
     1501 tcaccggaaa aatggcggaa actcagtgtc tcctaggagc gctgcttccg aaagaaagcg
     1561 atgctgcttt ttcgtgcggt aatgacgcag cattaaaggc caacgaaaaa tttagcatga
     1621 aagcggcgtt ggcagaatca agccaagaac tggaagatat cgagctcgaa gaagacgacg
     1681 ccgacattga ggcgtttgaa gcctacgaaa agcgcatgga cgaagaacgg gaacgtatgc
     1741 gcaaaaaaat cgaacaagaa aagtggcttg gtcaacagca gctcgccatt gcgacggtga
     1801 tggccgacgt gatcgaccca caacatgcag aacgaggtat cgatttcgag caaaaagcgg
     1861 tgaaacgatt cttttcaatt aagttttcta aaaatccaaa aaagggaatc gtgacgacgc
     1921 cgggaactcg ttaccagcca aagtacgaag acatacgtgc aaaggctgaa agtgacgaag
     1981 agcaacggcc gtaatgcaga aaaataggta tttcatgaat atgaaaacac cgcttcttcg
     2041 gattctggga gttcaaatat tggtggcgct cagtgtggtc gtgatgaccg cgctcgagcg
     2101 gcccgccttt tcggcagacc aactggtaaa agataagctg gaagcgttag ttggaatttc
     2161 gcaaaaaacg tcccgcgaat tttaccgact actttcggcg gaattacgaa aggaccaaga
     2221 aaaatcagga tcta
//
LOCUS       DAOMXO010000400         2233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28296_length_2233_cov_4.078512, whole genome shotgun sequence.
ACCESSION   DAOMXO010000400 DAOMXO010000000
VERSION     DAOMXO010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28296_length_2233_cov_4.078512"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..532)
                     /locus_tag="PLH57_08380"
     CDS             complement(110..532)
                     /locus_tag="PLH57_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRK02669.1"
                     /translation="MIKRIGVFSVFLFFTTIGQLQATFSFEENEALPIVYFNIVVNAG
                     SANDPKDKLGLTNMTYQMLLRGTQKRSKVEFDEALDNIGGRLGVDVRTEGTVFRGAVL
                     SENLKPFLDLVYEALTSPRFAAVETAKIKKEIEKPIKI"
     gene            complement(529..1854)
                     /locus_tag="PLH57_08385"
     CDS             complement(529..1854)
                     /locus_tag="PLH57_08385"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRK02670.1"
                     /translation="MLLTSCAHNPKTKTALGWKKIGSYPYLGANSVHRYEYPNGLKLL
                     VLEDHSAPVFTYHTWFNVGSKDEQPGLTGLAHLFEHMMFKATKNYPDGHFDRTLERAG
                     SEMTNAFTSRDYTAYVQSMPNEHLELLVKLESDRIVNLIVDDQALNKEREVVQNERRF
                     RNENNPDGKLYESIFTLAFKKHSYRWPVIGYAEDLERAKSAECYEFYKRFYAPNNATI
                     VVVGDVVPDDVAEVILKHYGHLTPTEIKRHKSPVEPPQIAERTQTLRQKIQVEKLVVG
                     YHIPPVTHPDTAALGVLRSVLATGKSSRLHRKLVDAGIASEISVDDGESRDPGLMVFF
                     VTLQKGRSAKRALKIIDQEIKAIQKSGVTQIEIDRAVAQNRFSLIDFQASLHYRAMFL
                     GYYESIAGDFKKGVELVNSYKTIRSKDVSRMAMQYLQPTQRSVVYGVPQ"
     gene            1939..>2233
                     /locus_tag="PLH57_08390"
     CDS             1939..>2233
                     /locus_tag="PLH57_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02671.1"
                     /translation="MMKQKQQQRALGSQSRRRRSRLLWLSVVTLVAAVILITLGPKIL
                     FETSHWITERVSRNFLLEEIEWQEIGDSNLSAFVTQELLWQTSGLRKGDEVLKV"
BASE COUNT          579 a          456 c          505 g          693 t
ORIGIN      
        1 attttatttg tctgatttac tttcactttt tttttcattt tcttaagtaa tttatgattt
       61 ttatgatttt cgtttttatt tcacttattt cttcttctca ctgctttttt catattttaa
      121 ttggcttttc gatttctttt tttattttgg ccgtttcgac tgccgcaaat ctcggagaag
      181 ttagtgcttc ataaactaaa tcgagaaaag gttttaaatt ttccgacaag accgcaccgc
      241 gaaagacagt tccctctgtg cgaacgtcaa cacccagccg accgccgatg ttatcgagag
      301 cttcgtcgaa ttccactttt gaacgctttt gagtacccct taaaagcatt tggtaggtca
      361 tgttagtcag ccccagcttg tctttcgggt cgttggcgga accggcgtta acgacaatgt
      421 tgaaataaac aataggtaaa gcttcgtttt cttcgaacga aaatgtcgcc tgtaattggc
      481 caatagtggt gaaaaataag aaaaccgaaa agacaccaat tcgtttaatc attgtggcac
      541 tccgtacacc accgaacgtt gggtgggttg caaatactgc atagccattc ttgaaacgtc
      601 ttttgatcga atagttttat aagagtttac gagttcgaca ccttttttaa aatctccggc
      661 gatcgattcg tagtaaccga ggaacattgc gcgatagtga agcgatgcct ggaaatcaat
      721 aagcgaaaaa cgattctgtg ctacggctcg atcgatttcg atttgagtaa ctccagattt
      781 ttgaattgcc ttaatttctt gatcaatgat tttgagagct cgtttcgcag agcgtccttt
      841 ctgaagagta acgaagaata ccatgagacc tggatctcgc gactcaccgt catctactga
      901 aatttcagat gctattccag cgtctacgag tttgcgatgt agacgagaac tttttccggt
      961 agccagaacg ctgcgtaata ctcccagcgc agcagtatca gggtgagtaa cgggtggtat
     1021 gtgataccct accaccaatt tttcgacctg aattttctga cgaagtgttt gggtgcgctc
     1081 cgcgatttgt ggtggttcaa cagggctttt atggcgttta atttcagtag gggtgaggtg
     1141 tccgtaatgc ttcaaaataa cttcggcgac atcgtcgggc acaacgtctc caacgactac
     1201 tatggttgca ttgtttggtg cataaaatcg tttgtaaaac tcgtagcact cagcgctttt
     1261 agctcgctcg agatcctcgg cgtagccgat caccggccag cgataggaat gtttcttaaa
     1321 ggcgagggta aaaatacttt cgtatagttt accgtctggg ttgttttcgt ttcggaaccg
     1381 tcgttcattt tgaaccactt cgcgttcttt gttgagggct tgatcgtcga cgattaaatt
     1441 cacgatgcga tcagattcga gttttacaag aagttccagg tgttcgttgg gcatactttg
     1501 tacataggca gtgtagtctc tactggtaaa agcattcgtc atttcggaac ccgctcgttc
     1561 caatgtccga tcaaagtgtc catctggata atttttggtc gccttaaaca tcatgtgctc
     1621 aaacaagtga gccaaacctg tgaggccagg ttgttcatct tttgaaccta cgttgaacca
     1681 ggtgtgatag gtaaaaactg gcgcgctgtg atcttccaaa accaaaagct tcaagccatt
     1741 aggatattcg taacgatgca ccgaatttgc accaaggtac gggtagcttc cgattttttt
     1801 ccaacccagg gcggttttgg ttttgggatt gtgggcgcaa gaagtaagca acatcagcgc
     1861 aacggcccca atgattttta tcgaaaattt tgaacacgaa aagttgcgag tcgtaatcat
     1921 agtagggtag cgtatacgat gatgaagcaa aagcaacaac aacgtgcctt gggcagtcag
     1981 agtagacggc ggcgatctcg tcttttatgg cttagtgttg ttacgcttgt tgcggcagtc
     2041 atattaataa cactcggtcc taaaatatta tttgaaacat cgcattggat taccgaacga
     2101 gtatctagaa actttttgtt agaagaaatc gagtggcaag aaataggcga ttcgaatttg
     2161 agtgcgtttg ttactcagga attattgtgg caaacctcag gtcttcgaaa aggcgatgaa
     2221 gttttgaaag tcc
//
LOCUS       DAOMXO010000401         2223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28480_length_2223_cov_5.399908, whole genome shotgun sequence.
ACCESSION   DAOMXO010000401 DAOMXO010000000
VERSION     DAOMXO010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28480_length_2223_cov_5.399908"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      259..359
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            669..1328
                     /locus_tag="PLH57_08395"
     CDS             669..1328
                     /locus_tag="PLH57_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRK02672.1"
                     /translation="MDERLKITEIFHSIQGESTLAGERFSFVRLTGCPLRCTYCDTTY
                     SYTGGQWRTVHEIVTEVLTHQTRWVCITGGEPLVQKNCLALVNALRSHDLNISIETDG
                     EEDVAPYAGLAKIILDIKTPGSGELAERAFANLDHLKATDEIKFVLTGRVDYEWAKTV
                     VTTHRLDARFTVLFSPVFKTTQDGLELKSLADWIIADSLNVRLQTQLHKHIWGTDRRG
                     V"
     gene            complement(1325..>2223)
                     /locus_tag="PLH57_08400"
     CDS             complement(1325..>2223)
                     /locus_tag="PLH57_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK02673.1"
                     /translation="KHLDSLLLENSDTILLKIKTITESVGAIAQKKIEFETVCEVAAL
                     EKTLVAEIFECVLHLVRNAVDHGIEVHDERVSAGKNPVSRVAIRLSVEQRNLCITVRD
                     DGRGIQTAKVLAVAKKRGLVPAAQKSLSETELARILFSPGFTTKEVATDLSGRGVGLD
                     AVEAVAVRNAGTVLVRSEEGQYTEFELRIPLRMYADRLLVCRAGGTLVAFAQKQIANI
                     TKSAAKSEAPVNDAGTLLGRKSNRITQILRLMSGAEWGVEDILGNDMYVVRCLNSQDL
                     DDQRVIGVTRVDSGEPVLVLVL"
BASE COUNT          564 a          558 c          545 g          556 t
ORIGIN      
        1 tattgcgttt ttaagtattt gatcgagcag gatttttcta aaaatttgac tgtactacgc
       61 aatgcgttac actaatcaat gggacaacta atagggtata gggaggttct agagcagaac
      121 aaaaacctaa aaaattacaa aggcttggcc ttgtttgaaa ccttccaatg atgggagacc
      181 gcgtgttcgt aagccaagga ggttgatgcg gaaaaggctt cagacaggcc agatgaagaa
      241 tgcgctgccc gcgcttccct ttgaaaaagg ctaactcaga ggaaactctg aggtcgcaaa
      301 gccatcgggg ctaccgaggc gacggtgacg tcgtcctcca ggccggccgg gttgccaaag
      361 gagtggtttc gagtcgctac cgatccaaac ccccctcata aagggcggta aggctcatag
      421 agtagacagc agggactcgg acatcgagac ttatcgctcc tattggtggt gatgagaaac
      481 tcgtcgtgca cagctcaagg ggaattattc gactgggacc tggccgctcg tcccccacac
      541 taacggccac gctccgcgtg gcctaggccg cccagtctga gttcttcata aatgacaggg
      601 tcgctgcttt acctagggaa tagggtgcag cggccctgtc gcgttttatg gtatagcgtc
      661 gaccgaagat ggatgaacga cttaaaatta ccgaaatctt tcacagcatc cagggcgaat
      721 caactttagc aggcgaacgc tttagttttg tgcgcctgac cggttgcccg ctgcgctgta
      781 cttattgtga cacgacttat tcctacactg gcggccagtg gcgaacggtt catgagattg
      841 tcaccgaagt gttaacgcac caaactcgct gggtgtgtat tactggcgga gaaccacttg
      901 ttcagaaaaa ctgtttagca ttggtgaacg cactgcggtc ccacgattta aatatttcta
      961 ttgaaactga cggtgaagaa gatgttgccc cttatgctgg tttagcaaaa attattctag
     1021 atattaaaac ccccggcagt ggtgagctcg cagaacgtgc attcgcaaat ctggatcact
     1081 taaaagctac cgacgaaatc aaatttgtac tcacgggtcg tgtagattac gagtgggcta
     1141 aaacagtcgt aaccacacat agactcgacg cacgtttcac cgtgcttttt tcccctgttt
     1201 ttaagactac acaggacgga ctcgaactta aaagtcttgc ggactggatt atcgctgata
     1261 gcttaaatgt tcgcctacaa actcaactgc acaaacacat ttgggggacc gatcggcgag
     1321 gagtttagag gaccaacacc agtactggtt cgcccgaatc gactcgcgta acaccgatga
     1381 cgcgttggtc gtcgagatct tgggaattta aacatcgcac cacgtacatg tcgttaccca
     1441 agatgtcttc gacaccccac tccgcgcccg acattaagcg tagaatctgg gtaattcgat
     1501 ttgattttct tcccaacagg gtccccgcat cgttgaccgg agcctccgat ttcgccgctg
     1561 atttcgtaat gtttgcgatc tgtttttgag caaatgcaac tagtgttccg cccgccctac
     1621 aaacaagtag ccgatcagcg tacattcgca atggaatgcg cagttcgaat tcggtgtact
     1681 gcccttcctc actacgcacc aggacagtac ccgcattgcg aacggcaacg gcttctaccg
     1741 cgtcgaggcc aactccacgc cctgaaagat cagttgcaac ctctttagtc gtgaaccctg
     1801 gtgaaaataa aattctcgcg agttcggttt cggaaagact tttttgagcg gcgggcacca
     1861 gccccctttt ttttgcgaca gcgagaacct tagcagtttg aattcctcgc ccgtcatcgc
     1921 gtaccgtaat acataggtta cgttgttcta cgctcaatcg aatagcgacc cgcgagacgg
     1981 ggtttttgcc ggcgctcacg cgctcatcgt gaacttcgat tccgtgatca acggcgttgc
     2041 gaaccaaatg caaaacacac tcgaagattt cggcaactag ggttttttcc aaagcggcta
     2101 cttcgcaaac agtttcgaac tctatttttt tttgagcaat cgctccgacg ctttcggtaa
     2161 tggttttaat cttaagaaga atggtgtcag agttttcgag gagcaacgag tctaaatgct
     2221 tgc
//
LOCUS       DAOMXO010000402         2222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28499_length_2222_cov_5.014767, whole genome shotgun sequence.
ACCESSION   DAOMXO010000402 DAOMXO010000000
VERSION     DAOMXO010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28499_length_2222_cov_5.014767"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLH57_08405"
     CDS             complement(<1..209)
                     /locus_tag="PLH57_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02674.1"
                     /translation="MGTVLRFSAKFVFKIPAIVLLIAVFALTLARPKLAHAQNEPSGE
                     DEYNFSWLDTDKKVFVLQNRKYKKA"
     gene            complement(253..>2222)
                     /locus_tag="PLH57_08410"
     CDS             complement(253..>2222)
                     /locus_tag="PLH57_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRK02675.1"
                     /translation="GDLEKVAVELLRNQEIADYFRKRYSDVFVDEFQDTNQAQCELVR
                     SISSRNQGLFVVGDVKQSIYRFRGSDVRVFEEFVAEVSSQRVLSTNYRSHPAIIQAVN
                     TVFDGVIRDFQPMRAFRKSEGGDRVFRVDANESAIGIAAAVARARSDGYAPGEIVLLL
                     RRIRGNEKLLEDLRSYGINTVFSLPRDLTENPKLFALCSLWITLHFSDLRGPALHAVA
                     ELGITNEEIEDLASRVSETIICTSLLAAIDAQWSMHQRFGITWSLFGDLIYLAHETGM
                     SGPGLAQEFLRLWQGRTPSGTLPLLVNSPEPPVTGSSSIRVTTVHSSKGMEYPVVILA
                     DLNDRRRSLGRVSLVKDHVWVFAPDSDGEIDRSSEHFKEALEEEKRESTDESNRLLYV
                     ALTRAKEQLYWVDVPGARGVRSGWCDLLHRKLLGQTAEITAASHSERFGEAEPTKVEP
                     IETINDSAPIVPVVPSGIARIPVTEYIKLQRVRGSNKRTFAKDDPSQSDRTAQKGTVV
                     HQYLQREAWEELWMYGDQENLDLSKFRAWLATESGRRVFDRKHAPIPQRLEREAAFEW
                     LHDGHILSGAIDRLIVWSDEAWIIDYKLIQRRGGLGREELQHQYGPQLSLYASIVKKM
                     THIARTRLFIIDLLASSGEVWVELRQVDDTL"
BASE COUNT          540 a          610 c          506 g          566 t
ORIGIN      
        1 tgggcttttt tatattttcg attttggaga acaaacactt ttttatcagt atcgagccaa
       61 gaaaaattgt attcgtcttc cccactgggt tcgttttgag cgtgtgccag tttcggtcga
      121 gccaaggtta acgcgaagac cgcaataagc agcactatcg ctggaatttt aaatacaaat
      181 ttagctgaaa acctgagtac ggtacccacg ttaaaacgcc ccctcgtgtt caaggttcag
      241 aaaacggtct ctctatagag tatcatcgac ttggcgcagt tcaacccaga cttcaccgct
      301 cgatgccaaa aggtcgatta taaaaagccg tgttcgcgca atatgagtca tttttttgac
      361 gatcgaggca tacaacgaaa gctgcggacc gtattggtgc tgaagttcct cgcggcccag
      421 tcctcctcgc cgctgaatca atttgtagtc aataatccac gcctcgtcgg accaaacgat
      481 caggcgatca atagcaccgg acaaaatgtg cccatcgtgt agccactcaa atgcagcctc
      541 acgctccaaa cgctgcggta tgggagcatg cttccgatca aaaacgcgtc gaccagattc
      601 cgttgccagc caagcccgaa actttgagag atcgagattc tcctgatctc catacatcca
      661 aagttcttcc caggcctctc gctgtaggta ctgatgaacg acggttcctt tttgtgcggt
      721 tcgatccgac tgactcggat cgtccttcgc aaacgtccgc ttgttcgaac cccttactct
      781 ttgcaatttt atatattcgg tcaccggtat tcgagctatg ccgctcggaa cgaccggaac
      841 tatcggagca ctgtcattta tcgtctcgat gggctcgacc ttcgttggtt ccgcttctcc
      901 aaatctctcc gaatgcgaag ccgccgttat ttcagctgtt tgaccgagca attttctgtg
      961 aagcaaatcg caccaacccg atcgcacccc tcgggcgcct ggtacgtcga cccagtacag
     1021 ctgctccttc gctcgggtaa gcgccacgta aagtaaacgg ttcgattcgt cggtcgattc
     1081 acgtttttct tcttctagtg cttctttgaa atgttcactc gaacgatcga tttcaccatc
     1141 actatcgggc gcaaacaccc agacgtggtc ttttaccaat gacacgcggc cgagcgagcg
     1201 tcttcggtcg ttcagatccg ctaggatgac gaccggatat tccatgcctt ttgatgaatg
     1261 caccgtggta acgcggatcg agctcgaacc cgtcaccgga ggctcaggtg agttcacgag
     1321 taacggtagt gtgccgcttg gtgttcgccc ctgccacaat cgcaagaact cttgcgcgag
     1381 tcctgggccg gacatgccgg tttcgtgggc caaataaatc aaatcaccga aaaggctcca
     1441 tgtaattcca aaccgctggt gcattgacca ctgcgcatca attgccgcaa gtaaactggt
     1501 acatatgatg gtttccgaca cccgtgaggc gaggtcctca atttcttcgt tcgtaatacc
     1561 cagctctgca actgcgtgca gggcaggccc ccgtaagtcg ctaaagtgca acgtgatcca
     1621 caaactacat aaagcgaaca gtttggggtt ctcggttaga tcccgcggaa gcgaaaatac
     1681 agtattgata ccgtatgaac gaaggtcttc gagtaatttt tcgttgcccc gtattcgacg
     1741 caacaacaac acaatttcac cgggtgcgta accatcagaa cgcgctcttg ctactgcagc
     1801 cgcaattcca atcgccgact catttgcgtc tactcgaaac acccgatctc cgccttccga
     1861 tttgcgaaat gcccgcatcg gctgaaaatc tcgaatcacg ccatcaaaaa cggtgttcac
     1921 cgcctgaatt atagcgggat gggagcgata attggtcgaa agtactcgtt gggaacttac
     1981 ttcggcgaca aattcttcga acacgcgaac gtcgctgccc cgaaagcgat aaatactttg
     2041 ttttacgtcg cccaccacga agagaccttg gtttcgcgag cttatgctgc gtacgagttc
     2101 acactgagct tgattggtat cttgaaactc atcgacaaac acgtcgctgt atcgtttccg
     2161 aaaataatcg gcgatttctt gatttcgaag taactcgact gcaacctttt ctaaatcgcc
     2221 gt
//
LOCUS       DAOMXO010000403         2218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28571_length_2218_cov_5.457235, whole genome shotgun sequence.
ACCESSION   DAOMXO010000403 DAOMXO010000000
VERSION     DAOMXO010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28571_length_2218_cov_5.457235"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..743)
                     /gene="pgsA"
                     /locus_tag="PLH57_08415"
     CDS             complement(12..743)
                     /gene="pgsA"
                     /locus_tag="PLH57_08415"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRK02676.1"
                     /translation="MLLSKIMTTSNTENNSQAGNIPDAPDQGYDNLPNRITALRIAFV
                     PLVVFFLTKDSPLYGFIAALLFGVAAITDYFDGYYARTRKLVTVLGKLLDPLADKFLV
                     VSCLIMLMHLDRIHPYVVILLICRELAITGLRAIASAEGIVIAASNLAKWKTVTQMIA
                     IPMLMLHETYLHVPMQLLGTVFTYLSLLISLWSAKDYIVDFFKTTRAMAREKREGKRA
                     RRLAKRAERLQKLPISFPTCSCSSV"
     gene            777..1424
                     /locus_tag="PLH57_08420"
     CDS             777..1424
                     /locus_tag="PLH57_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02677.1"
                     /translation="MQAQSGPKQWLTLVWLVVLVGAPVRVDATGILWSKHKPTPESLV
                     KEKTLKRVREGSASVTNANWNDSDDFTFLSSTLVRTPLRFARPHVVDPNFYARLTDAI
                     KKLTYDPRTKTAEIIGEAGGLTMHSWLKMDDRLWDEVHFTIIKGDLKGMRIRTYLWEE
                     SGKTLIIGKGELKNARSKFGGLVARVLPTVAEVVIDVATGRFRGYIEEEYQKKRL"
     gene            1458..>2218
                     /locus_tag="PLH57_08425"
     CDS             1458..>2218
                     /locus_tag="PLH57_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRK02678.1"
                     /translation="MTEKKKTTRKTKGGAKKNSSASTNTKSQTDATDRANLRLVERRR
                     HPRFLLSREQFREIKTGKTFPVYDLSMNGLSIRSDEQYWPAGSLMSGVLNIHPESIEV
                     TTRLLGYYGDRAALRLESVSTYARSVLEKALSPKRLGQSLHLVREHLPLADYWYHGVC
                     NTDVLLRLNGEGKIAKAEVFFSNSYWSWTEHSDLMATGVCQSIGEKEREATLIAEEPV
                     KLESVSLEMDGAVDPVKVRWAKGILESAPLEPLLK"
BASE COUNT          602 a          511 c          588 g          517 t
ORIGIN      
        1 cgccgccatc gtcagacgct gctgcatgag caggtcggga acgaaatagg gagtttttgt
       61 aagcgttccg cgcgcttagc cagacgcctt gctcgttttc cttcacgttt ttcgcgggcc
      121 atcgcgcggg tagttttaaa gaaatcgacg atataatcct tcgccgacca gagcgaaatc
      181 aaaagtgaaa ggtacgtgaa aacagtaccc agcaactgca ttggaacatg caagtatgtt
      241 tcgtgcagca tcaacattgg aatggcgatc atttgggtca cggttttcca ttttgccaga
      301 ttgctggcag cgatcacgat tccttcggcc gatgcaatag ctcgcaggcc agtaatggct
      361 aactcacggc aaatcaagag gatgacgaca taggggtgaa tgcgatccaa gtgcatgagc
      421 atgatcaaac agctcactac aaggaacttg tcggccagag gatctaacaa ttttcctagt
      481 acagtgacca gttttcgagt tcgcgcgtag tacccgtcga aataatcggt aatcgcggca
      541 accccaaata aaagagcggc tataaaacca tagagcggcg agtctttcgt tagaaaaaaa
      601 accactaacg gcacaaacgc gatacgtagg gccgtgatgc gattcggtaa attgtcatag
      661 ccttgatcgg gagcgtccgg aatattgcca gcctgcgaat tgttttcagt gttcgatgtc
      721 gtcatgatct tgctcaagag catactccgc gtacaatcaa tagtcgaggt cttctaatgc
      781 aagctcagag tgggcctaag cagtggttaa ccctggtatg gttggtagtc ctagtgggtg
      841 cgcctgttcg ggtggatgcc actggcatcc tctggtcgaa acacaaaccg accccagaga
      901 gtcttgtgaa ggaaaagact ctaaaaaggg ttcgtgaagg cagcgcctca gtaactaacg
      961 ccaactggaa tgattccgat gattttacgt ttctttcgtc gactctcgtg cggaccccct
     1021 tgcgtttcgc gcggccccat gtggtggatc caaattttta cgcccgactc accgatgcga
     1081 ttaaaaaatt aacctacgac ccacgcacca aaaccgcgga aataattggt gaggccggcg
     1141 gactcacgat gcactcgtgg ttaaaaatgg atgatcgact ttgggatgaa gtgcatttca
     1201 cgatcattaa aggtgatctc aaaggaatgc gcatccgaac ctatttgtgg gaagagtccg
     1261 gtaaaacact gattatcgga aaaggagaac tcaaaaatgc ccgttccaaa ttcggtggtt
     1321 tagtcgcgcg agttttgccc acggtagcgg aggtggtgat tgacgttgca acgggtagat
     1381 tccgcggtta cattgaagag gagtatcaga aaaagcgact ctagatctta cgggaactca
     1441 aaaaaagata aacctcgatg accgaaaaga agaaaacgac gcgaaagacg aagggcggag
     1501 ccaagaaaaa tagttctgcg tcaacaaata caaagtcgca gactgacgcg acggaccgag
     1561 caaatttgcg tttagttgaa cgacgtcggc acccgcgttt tttattgagt cgagagcaat
     1621 ttcgcgaaat caaaaccgga aaaacttttc cggtctacga tctcagcatg aacggccttt
     1681 cgattcgaag cgatgaacaa tattggccag cgggttcctt gatgtcgggc gtacttaata
     1741 ttcatccaga aagcatcgaa gtcaccacta ggttgcttgg ctattatggc gatcgcgcag
     1801 ccttgcggtt agagagtgtc tctacctacg cgcgatcggt gctcgaaaaa gcgttgagcc
     1861 ctaagcgttt ggggcaaagt cttcatttag tgcgcgaaca tttgcccttg gcagattact
     1921 ggtaccatgg agtttgcaac accgacgtgt tgttgcgcct caacggtgaa ggtaaaatcg
     1981 ccaaagcgga ggtgtttttt tcgaactcct attggagttg gactgaacac tcggatctta
     2041 tggccacggg agtgtgtcag tcgattgggg aaaaggaacg cgaagccacc ttaatcgcag
     2101 aagagcctgt aaaactcgag tccgtctcgc ttgaaatgga cggtgccgtg gatcctgtga
     2161 aagtgcgttg ggccaaagga attctcgagt ccgcgccgct agagccgctg ttgaaaca
//
LOCUS       DAOMXO010000404         2216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28610_length_2216_cov_5.826932, whole genome shotgun sequence.
ACCESSION   DAOMXO010000404 DAOMXO010000000
VERSION     DAOMXO010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28610_length_2216_cov_5.826932"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PLH57_08430"
     CDS             complement(<1..666)
                     /locus_tag="PLH57_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRK02679.1"
                     /translation="MSGYTQVTPPKNGQKIKMRADMTLEVPDNPILPYIEGDGTGPDI
                     WRASQRVLDAAVEKAYQGKKKIEWFEVYAGEKSKNKFDNWLPEDTITAFKEYLVGIKG
                     PLTTPVGGGIRSLNVALRQMLDLYVCLRPVRYFKGVPSPVKRPEKVDMVIFRENTEDI
                     YAGIEWQSGTPEAQKVIQFLEKDMGVKKIRFPKTSGIGIKPVSQGGSRGCNPGLRIPH
                     SCPI"
     gene            848..1525
                     /locus_tag="PLH57_08435"
     CDS             848..1525
                     /locus_tag="PLH57_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK02680.1"
                     /translation="MQASYSARYEDRLFCIDQHLIGSMRNFVYLITSDKQSFIVDSQK
                     DLSAWEKTRDELGSTLKGVLLTHTHHDHVAGVDELAEKYDVPFYLHRGDIRGLNKKSQ
                     LVQSRTKYLNEGDKLRLGKIDIEILHTPGHSAGECCFLIRGEKHWHLFTGDTVFVGNV
                     GRTDLETGSTPVMLQTLARLRALPASTVIYPGHDYGATWTTTIERELRESAAFTAQTV
                     AEFDALP"
     gene            1538..>2216
                     /locus_tag="PLH57_08440"
     CDS             1538..>2216
                     /locus_tag="PLH57_08440"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRK02681.1"
                     /translation="MIYKSFVKPLLFRLDPEVAHNLMVRVCSILWQFRLGRALSRFIF
                     SRKTAFSTRSTNLAGLKLKNPIGLAAGFDKSGELAIGLETLGFGFIEIGTVTPRPQAG
                     NPKPRLFRDPVNLALINRMGFNNDGAIVVAERLRRSKKFISIPVGVNIGKNKDTPIER
                     AVEDYRECARAFRGLADYLTVNISSPNTPGLRDLQNVAFLTELATSLNAEQLGCPVFL
                     KLAPEVTG"
BASE COUNT          540 a          568 c          536 g          572 t
ORIGIN      
        1 gattgggcac gaatgcggga tccgtagccc ggggttgcac ccccgggacc ctccttgcga
       61 aactggcttt attccaatgc cgctggtttt cggaaacctg atttttttta cgcccatgtc
      121 tttttctaaa aattgaataa ctttttgggc ctctggagta ccagactgcc attcgatgcc
      181 agcatagatg tcttcggtgt tttcgcgaaa gatcaccata tcgacttttt cgggccgctt
      241 cacgggacta ggcacgccct tgaagtaacg aacaggtcgt aaacaaacat agagatcgag
      301 catctgacga agagctacat tgagcgaacg aatgccgccg cccaccggag tagtgagcgg
      361 gcccttaatg cccaccaaat attctttaaa cgcagtaatt gtgtcttcag gaagccagtt
      421 gtcgaatttg tttttcgatt tttcaccggc gtagacttcg aaccattcaa ttttcttttt
      481 accctggtag gccttttcga ccgctgcatc gagaactctt tgggaggctc gccaaatatc
      541 agggccggtg ccgtcgcctt cgatgtacgg caaaatcgga ttatcgggca cctctagcgt
      601 catgtccgca cgcattttaa ttttctgacc atttttgggt ggggttactt gggtatagcc
      661 gctcatgaaa tgcactccga tctaataggt ataggctcaa ttattgtaat tgtgtctaat
      721 gaggtagcaa gaaatttaac ggagcgtcaa ttgggaagcg caacaccttg cgagttgttt
      781 gcccctaacg cggcgtttac ataaaatgct atcttctact aacccttttt ttagctataa
      841 gggagccatg caggctagtt actcggcacg ttacgaagac cgattgtttt gcatcgacca
      901 acacctcatt ggatcgatgc gcaattttgt ttacttaatc acaagtgaca aacagtcctt
      961 cattgtggat tcgcagaaag acctgagtgc gtgggaaaaa acacgggacg agctcggatc
     1021 caccttaaaa ggtgttttac taacacacac ccaccacgat catgtcgctg gagttgacga
     1081 attagccgaa aaatacgacg tcccgtttta cctacaccgc ggggatattc ggggactaaa
     1141 taaaaagtcg cagctcgttc aaagtcgtac gaaataccta aacgaaggtg acaaattgcg
     1201 cctcggaaaa atcgatatcg agattttgca caccccaggc cacagcgctg gtgagtgctg
     1261 ctttctcatt cgcggggaaa aacattggca cctattcacc ggcgacacgg tgttcgtggg
     1321 caacgtcggg cgaaccgact tggaaacggg atctaccccc gtcatgctgc aaacgcttgc
     1381 gcggctgcgt gcactgccgg catccaccgt catttacccc ggccacgatt acggtgcgac
     1441 ctggaccact accatcgaac gcgaacttcg cgagtctgcc gcgtttaccg cccaaaccgt
     1501 tgccgaattc gatgccctgc cataagttgt cagagccatg atttataaat cgttcgtaaa
     1561 accactgctt tttcggcttg atcccgaagt tgcgcacaat ctgatggtta gggtttgctc
     1621 cattctctgg cagtttcgct tgggccgagc tctgagccgt tttatttttt cccgaaagac
     1681 tgctttttca acccggtcaa cgaatctggc aggactaaaa cttaaaaacc ccatcgggct
     1741 tgctgcgggt ttcgataaaa gcggcgagtt ggccattggc ctcgaaacgc tgggttttgg
     1801 tttcatagaa atcgggaccg ttacgccacg acctcaagcg ggtaatccga aaccgcgact
     1861 ttttagagac cccgtgaatt tggccctcat aaatcgcatg gggtttaata acgacggtgc
     1921 gatagtcgtg gcggaacgac tgcgcagaag taaaaagttt atttcgattc cagttggcgt
     1981 gaacattggc aaaaacaaag acacgcccat tgaacgcgcg gttgaagatt atcgtgaatg
     2041 tgctcgcgcg ttccgaggcc tagccgatta tcttacggtc aatattagtt cccccaacac
     2101 accgggcctt cgcgatcttc aaaacgtagc ctttttgact gagttggcga caagcctaaa
     2161 tgccgaacaa ctcggctgcc ccgtgtttct taagcttgcc ccagaggtca ctggcg
//
LOCUS       DAOMXO010000405         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28810_length_2205_cov_5.626977, whole genome shotgun sequence.
ACCESSION   DAOMXO010000405 DAOMXO010000000
VERSION     DAOMXO010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28810_length_2205_cov_5.626977"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PLH57_08445"
     CDS             complement(<1..1167)
                     /locus_tag="PLH57_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRK02682.1"
                     /translation="MSKKAKIKIAERAPLKLVPAVAPMPPFVRVTKNEKVAEQLEALF
                     ENANDAILVVDLKKKEIAAANLQALELTGYLRSEIVGDSVSKLFPTNRELPEHSQGLA
                     LTHLENSGFFEDLVLQNKDGMYRFVSLSVKVLSLGRNHVAMCIFRDVTEKKNMERELI
                     TKHGELRNAYLSLEKANAEHRAMQDTLVESGKLAALGELAAGIAHELNQPLTGVKGFA
                     QEALATIPNEKKLESIKTCLEEVVKGAEKMERIITHLRKFTRKSTEDFEWVDVHEVID
                     ESLVMLAKQFRSRGIEVIREYGSDVPKIYCNPFQLEQVFINLATNARDAIESKRAGRG
                     KIVIRTLRHSSKIVEVQFEDDGTGITEAARSKIFNPFFTTKEVGKGMGLGLSITH"
     gene            complement(1164..>2205)
                     /locus_tag="PLH57_08450"
     CDS             complement(1164..>2205)
                     /locus_tag="PLH57_08450"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRK02683.1"
                     /translation="NVTVGFTDTAYGRNSRMGNPKLGPHLRNEAQIPKGFNQLLEKLH
                     DIPTLPIVATKVTELINNPNSSSADIANVLKKDQVLTAKVLKLINSPYYGIPGGVTDV
                     KRALAFLGFNTLAQLVLGLSVFSLFPGEGSEEFPMLDFWRHALATGVASEVIAKKIKH
                     PKPEECFTCGLLHDIGKIVMHQVARETLFTIVRHARKNQISFTQAESELDAPSHAFLG
                     EHIAAKWGLPQVIRTTIRYHHVDVRNSETILPSAKPSIYIVAVANALAIEMKIGHSGD
                     YSDGGLQPYMYEMLGLQASDMETIQNIVREEMRRAGAFLSAAGGGAAREDDTKADSNG
                     APPSRGEGSTAA"
BASE COUNT          492 a          566 c          508 g          639 t
ORIGIN      
        1 gtgggtaata cttagcccca gtcccatgcc ctttccgact tctttggttg taaaaaacgg
       61 attaaaaatt tttgatctcg ccgcctcggt aatgcccgtg ccatcgtcct cgaattgcac
      121 ttcgactatt ttcgaagaat gtcgaagcgt tcgaataaca attttacctc gccctgcgcg
      181 cttgctctcg attgcgtcgc gggcattggt ggctaaatta ataaagacct gctctaattg
      241 aaatggatta caatatattt taggtacgtc cgagccatac tcccgtatca cttcgatgcc
      301 ccgagatcta aactgttttg ccaacattac cagggactca tctatgactt catgcacatc
      361 gacccactca aaatcttcgg tgctcttacg ggtaaatttc cgtagatgag taatgatacg
      421 ctccattttt tcggcacctt ttacgacctc ttctaaacac gttttgatac tttctagttt
      481 tttttcgttc ggtattgtcg ccagtgcttc ttgggcaaac cccttcactc cagtgagcgg
      541 ttgattaagc tcgtgtgcga tacccgctgc gagttcgcct agcgctgcga gtttacccga
      601 ctcgaccaaa gtgtcctgca tagctcggtg ttccgcattg gctttttcta aacttaggta
      661 cgcgtttcgc aactcgccat gtttcgtaat caattctcgt tccatgtttt tcttctcagt
      721 aacgtcccga aaaatgcaca tagctacgtg attgcggcca agcgagagca cttttactga
      781 caaactcaca aaacggtaca tcccgtcttt gttttgcaaa acgagatctt cgaaaaaacc
      841 actgttttca aggtgtgtca gagccaaccc ctgggaatgc tctggtagtt cgcgattggt
      901 cggaaaaagc ttgctgacag aatcaccgac gatttccgaa cgcagatatc ccgtgagttc
      961 gagagcctgt aaatttgcgg ctgcgatttc ctttttcttt agatcgacca ccaaaatagc
     1021 atcgttggcg ttttcaaaaa gcgcttcgag ctgttccgct actttttcgt tcttggttac
     1081 ccgaacaaac gggggcatcg gcgccactgc gggcaccagt ttaagagggg cgcgttcggc
     1141 gattttgatt ttcgccttct tactcatgcc gccgtactcc cttcaccacg gctcggaggc
     1201 gcaccgttgc tgtcggcttt agtgtcatct tcacgggcgg cgccaccgcc ggcagccgat
     1261 aagaacgcgc ctgcgcgccg catttcttcc cgtacaatat tttgaatcgt ctccatgtca
     1321 gatgcttgca accctaacat ttcgtacatg tagggttgta gtcctccgtc ggagtaatct
     1381 cccgagtggc cgattttcat ttcaatagcg agcgcatttg ctaccgccac aatgtagatc
     1441 gacggtttag cactcggtaa aattgtttct gaattgcgca catcgacgtg atggtaacga
     1501 atcgtcgtgc gtatgacttg tggaagcccc cactttgccg ctatgtgttc tccaagaaaa
     1561 gcgtggcttg gcgcatcgag ttcgctttca gcctgagtaa agcttatttg attcttgcga
     1621 gcgtggcgta cgatcgtaaa cagagtctcg cgcgccactt gatgcataac tatttttcca
     1681 atgtcatgta atagtccgca cgtaaaacac tcctctggtt tgggatgttt tattttctta
     1741 gcaatcacct cactcgcgac tccagtggcc agtgcgtgac gccagaaatc gagcatgggg
     1801 aattcttcag aaccttcgcc cggaaacaaa gaaaacacgc ttagtcccag taccagctgc
     1861 gcaagggtgt tgaagcccaa aaacgctaac gcacgtttta cgtcggtcac gccgccagga
     1921 atgccgtagt acggagagtt aataagtttt agaactttcg ccgtgagcac ttggtctttt
     1981 tttagaacat tcgcaatgtc ggctgaactc gagtttgggt tgttgataag ttcagtgact
     2041 ttagttgcca cgattggcag cgtcggaatg tcgtgtagtt tttcgagcaa ctggttaaaa
     2101 ccctttggaa tctgggcttc gttgcgtagg tgtggaccga gttttggatt ccccatgcgg
     2161 ctgtttctcc cgtaagccgt atcggtaaaa cccacggtca cattg
//
LOCUS       DAOMXO010000406         2202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28867_length_2202_cov_5.010713, whole genome shotgun sequence.
ACCESSION   DAOMXO010000406 DAOMXO010000000
VERSION     DAOMXO010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28867_length_2202_cov_5.010713"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..>2202)
                     /locus_tag="PLH57_08455"
     CDS             complement(1..>2202)
                     /locus_tag="PLH57_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRK02684.1"
                     /translation="LMTQERQSRPSIIYSDIFVIRKDAIFENQFLLDRLADLRIPFRF
                     ENGKLEYAARSFEYPETLRDPGVHFPVANALIAVETSQGLISAISVDGESHDAIALEP
                     TTIAQLSGNSLSIRDYVKIGEIPTQLLQAIIAIEDQRFLDHPGFDLRSLMRAMWVNLQ
                     SGSFSQGGSTITQQLIKNLLGTRQKTVARKARELVLAILMEARYTKEQILEKYLNEVY
                     FGQIGSLEVHGVAEAARYFFAKSIDKLSLAEMALICGVIRGPGLYSPYKNMQKAIERK
                     DTVLGKMMELRIISGEDYELARGEQLTFAPPTQAGNRAPYFVDYVKAQVLDQLEDRFS
                     ADDLSAQGLRIYTTLDLQLQKKADAAVAAWVRELESRHKIERPLRLEGLLVVGDQKSA
                     SLRALVGGRSYAETNFNRVLNMKRQAGSTFKPIIYLAAFEKEKDASGVSYTPAYLIDD
                     EPWTHKYVGDQQWSPRNYEKTYRGRIGLREAFVNSINIPAAKIGVDVGPAAIASLARK
                     LGVQSAIPEVPSVSLGSVDVSPLDLLSVYTTFANQGAWKEMTAVTAITDASGGILARF
                     VAKTGQAAEQANVDLLNHLLNAVTTEGTAKSLPALGYKKTSFGKTGTTSFYRDAWFAG
                     FSEDLVAVAWAGFDELKVPDSEDTKSLETFKSPAQLTGAGSALPIWARFFATARPAAL
                     AAEQPLLETDLEKQSIDRSTGLISKSTCPNEWVYEEKFKPGTLPEKECDQH"
BASE COUNT          546 a          547 c          528 g          581 t
ORIGIN      
        1 ttagtgttgg tcacactctt tttcaggcag tgttccgggt ttgaattttt cttcgtaaac
       61 ccactcgttg ggacaagtcg attttgaaat caacccggtc gatcgatcga tgctttgttt
      121 ttcgaggtca gtctccagca gcggctgttc cgcggccaat gcagcaggtc gagctgtggc
      181 aaaaaacctg gcccaaattg gtaacgctga acctgcaccc gtaagttgcg cgggagattt
      241 aaatgtttca agacttttcg tgtcttcact gtcaggcact tttagttcgt caaacccagc
      301 ccaggcgacg gctactaggt cttcggaaaa accggcaaac caagcatcgc gatagaaact
      361 agtagtacca gtttttccaa atgaggtttt tttgtaaccc agtgccggca aactttttgc
      421 ggttccctca gtagtcactg cgttcaacaa atggttaagc aggtccacgt tcgcctgttc
      481 ggcggcctgt ccggttttgg ccacaaatcg tgcgagaatt ccaccggagg cgtcagtaat
      541 cgcagtgacc gccgtcattt ctttccaggc accttgattc gcaaatgttg tataaacact
      601 caacaaatcc aacggactaa cgtccaccga acctaggctt accgatggta cttcgggtat
      661 ggctgactgc actccgagct tacgcgccag cgatgcgatc gcagcgggtc ctacatcaac
      721 accaattttt gctgcaggaa tattaattga atttacaaac gcttcccgca aaccaattcg
      781 gccgcggtag gttttttcat aattgcgagg actccactgt tgatcgccta cgtatttgtg
      841 agtccacggt tcatcgtcaa tgaggtaagc aggagtgtag gagacgcccg aagcatcttt
      901 ttctttttcg aacgccgcta aataaataat gggcttaaaa gtactacccg cttgtcgttt
      961 catatttagc actcggttaa aattagtttc agcgtaggaa cgtcctccca ctaaggctcg
     1021 caaagaggcc gacttctgat cgccgactac aagcaagccc tcaagtcgaa gcggtcgctc
     1081 tattttgtgc cgtgactcca gctcgcgtac ccacgcggct accgctgcgt cagccttttt
     1141 ttgcagctga agatcgagcg tggtgtaaat gcgtaaacct tgcgcactta gatcgtcggc
     1201 cgaaaaacga tcctcaagtt ggtctaatac ctgcgctttt acgtaatcga caaaatatgg
     1261 ggcgcgatta ccggcttggg tcggtggcgc aaacgtgagc tgttctccgc gggccaactc
     1321 atagtcttcg cccgaaataa ttcggagttc catcattttt ccgagcacgg tgtctttgcg
     1381 ttcaatggct ttttgcatgt tcttgtacgg agagtacaac ccagggccgc gaataacgcc
     1441 gcaaattagg gccatttcag cgagagagag cttgtcgatt gattttgcaa aaaagtagcg
     1501 ggcggcttct gccacgccgt gaacttcaag tgaaccaatt tgcccaaaat aaacttcgtt
     1561 caggtatttc tctaaaattt gctctttagt gtaccgagcc tccatgagaa ttgccaaaac
     1621 cagttcccgt gccttgcggg ccacggtctt ttgccgagta cccaaaaggt ttttaatcag
     1681 ctgttgagtg atcgtacttc cgccttggga aaaacttcca gactgcaaat ttacccacat
     1741 tgcgcgcata agactgcgta agtcgaagcc gggatggtcc aagaaacgct ggtcttcaat
     1801 cgcaataata gcctgcagaa gttgagtggg aatttctcca atttttacgt aatcgcgaat
     1861 agacaaagag ttgcccgata actgagcgat ggttgttggt tcaagagcaa ttgcgtcgtg
     1921 agattcgcca tctaccgaaa tagcagagat taaaccctgg gacgtttcaa ccgcaatcag
     1981 tgcattggcc accggaaaat gaacaccggg atctcgcaac gtttccggat actcgaacga
     2041 gcgagcggcg tattcgagtt tgccgttttc aaaccgaaac ggaatgcgca aatcggctaa
     2101 gcggtcgagt aaaaactgat tttcgaaaat cgcgtcctta cgtattacga atatatcgga
     2161 atatattata gatgggcgcg attgccgctc ttgagtcatt ag
//
LOCUS       DAOMXO010000407         2202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28868_length_2202_cov_4.432697, whole genome shotgun sequence.
ACCESSION   DAOMXO010000407 DAOMXO010000000
VERSION     DAOMXO010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28868_length_2202_cov_4.432697"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PLH57_08460"
     CDS             complement(<1..1016)
                     /locus_tag="PLH57_08460"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRK02685.1"
                     /translation="MGRSFLTLNILLFIISSSSLANSGRKPASESPSAKAPFTGVVVL
                     TDGTVYSQRGSVHYTRPNLGRKIKVNDFGQYQDAPAMPVFVKFATDSQRYLKPNGLVF
                     PGDVLLTAGEGFTKILFRDNSLVDMGPGAALEIAKFDVDQDLSKKRDVELRIAYGKFR
                     VIVADPLRDGQTFKVYTPNTMMNVRGTEFIVNVFTDKEGLSQTDVVCLHGQVSVDLAK
                     PTTKGLVYHQTAVVNPGVRFRTEGHNGLTREVMSKALNPAQLREAVKSTSPLVNALAT
                     YAAVLPRALDVPGTGVSNIAQARRYPDFKALANKPTGEELLQPSRRIASLKNFDTPEK
                     DFDVS"
     gene            complement(1095..>2202)
                     /locus_tag="PLH57_08465"
     CDS             complement(1095..>2202)
                     /locus_tag="PLH57_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02686.1"
                     /translation="AVWKPASPLNAATNPPVQNTTIGNYHLFSSLSPRIWSPVGEFSS
                     SYWTAGVSLLGWDDLDQLRYSLTPWYDSFSKKPMGNASTRHRIGTFYLGLSAEREVTD
                     RFSFASGLEFYSIDTTFGASLMRPWTWIEWQFTPTVVFERNQASFGGSFLHRTFEPDY
                     RAGLDLRLSSIESFDFSIGPDEGFGAVLSGRKFWSSGDQPLKTYAQMRKIFSLWSQHT
                     VLDTQIFGAIAHEASYDIPDSIIEVGGSGDSSRLDLPLRGYPSNALLGNRAAAVQFEL
                     RIPISQVFRGTDTFPMFNRNFGAFVFYDAAKVRETDDEWRGYSAGAGGGLVFNLTAFY
                     HVPFNLKFQYARGLHRAHRGESAFNILIGGGLPF"
BASE COUNT          583 a          521 c          575 g          523 t
ORIGIN      
        1 gaaacatcaa aatctttttc gggagtatca aagtttttca aactcgcgat tcgtcgcgat
       61 ggttgcaaaa gttcctctcc ggttggttta tttgctaacg ctttaaagtc cggatacctc
      121 cgggcttgcg cgatgttcga gactccggtt cctggtacgt cgagcgctcg cgggagcacg
      181 gcagcataag tggcaagggc gttgaccagt gggctcgtgg actttactgc ttcgcggagc
      241 tgcgccggat tgagtgcttt actcatgact tctcgtgtga gcccattgtg cccctcggtc
      301 cggaacctaa cgcctggatt caccacggca gtctggtggt agactaggcc cttggtggta
      361 ggctttgcca gatcaactga aacctgaccg tgcagacaaa caacgtcagt ttgcgagaga
      421 ccttctttgt cggtgaatac gtttacgata aattcagtac cacgcacatt catcatggtg
      481 ttgggagtgt aaaccttaaa ggtttggcca tcgcggagtg gatcggccac aataacccga
      541 aattttccat aggcgattcg caattcaacg tcgcgttttt tactgaggtc ctgatcaaca
      601 tcgaatttcg ctatttccag agcggcgccg gggcccatgt cgacgagcga attgtcgcga
      661 aacaatattt tggtgaaccc ttcgccagct gtcaaaagca cgtcgcccgg aaagacaagt
      721 ccgtttggtt ttaagtaacg ttgggaatcg gtagcgaatt tcacaaatac cggcatggcg
      781 ggagcgtctt ggtattggcc aaaatcattg actttaattt tgcgtcctaa attcgggcga
      841 gtgtagtgaa ccgaacctct ttgagaatat accgttccat cggtcaacac tactactccg
      901 gtgaacgggg cttttgcgga gggcgattcc gacgctggtt tgcggccgga attggccaga
      961 gaagaactgc tgatgataaa caacaaaata tttagtgtta aaaaacttcg gcccatctat
     1021 ttaaagatag cgtaaaccgg ccgcgagcgg cacgggcaga ttttgccgtt tgagtttcat
     1081 ttttgcctgc cggactaaaa cggcaagccg ccaccgatta ggatattgaa tgcgctctct
     1141 ccacgatgtg ctcggtgaag accgcgggca tactgaaatt taaggttaaa tggcacgtgg
     1201 taaaaagccg tgaggttaaa caccagacct ccgccggctc ccgcagaata accgcgccac
     1261 tcgtcatcgg tttcgcgaac tttagcagca tcataaaaca cgaaggctcc gaagttacga
     1321 ttgaacattg gaaaagtgtc ggtaccccga aaaacctgtg aaattggaat gcgcagctcg
     1381 aactgcacgg ccgcggctcg atttcccagc aatgcattgg aaggatagcc acgcagaggt
     1441 agatctaagc gtgaagaatc gccgctgcct ccaacctcta taatagagtc aggaatgtcg
     1501 taggatgctt cgtgggcgat ggcgccaaaa atctgagtgt ctaagaccgt atgttgagac
     1561 cagagtgaaa aaatttttcg catttgcgcg taagttttca gaggttggtc tccagaactc
     1621 caaaatttac ggccggacaa gactgctccg aagccttcgt cggggccaat cgaaaaatca
     1681 aaagactcga tacttgataa acgaagatct aaaccggccc gataatctgg ttcaaacgtg
     1741 cggtgaagaa aactaccgcc gaacgaagcc tgattgcgct caaaaactac ggtgggagtg
     1801 aactgccatt caatccaggt ccaaggtcgc attaaagatg caccgaacgt ggtatcgatt
     1861 gaataaaatt cgagcccact ggcaaatgaa aatcgatcag tcacttcgcg ctcggcagaa
     1921 aggccaagat aaaacgtacc gattctgtgg cgagtagagg cgttccccat gggttttttt
     1981 gaaaaagaat cataccaagg ggtcaaggaa tatctcagtt gatcgaggtc atcccaacct
     2041 aacaaactaa caccggcggt ccagtagcta gaggagaatt caccaacagg gctccaaatt
     2101 cttgggctca acgaggaaaa tagatggtag ttcccaatag tggtgttttg gacaggggga
     2161 ttggttgcag cgttcaacgg acttgcgggt ttccagacgg cg
//
LOCUS       DAOMXO010000408         2202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28871_length_2202_cov_3.974849, whole genome shotgun sequence.
ACCESSION   DAOMXO010000408 DAOMXO010000000
VERSION     DAOMXO010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28871_length_2202_cov_3.974849"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLH57_08470"
     CDS             <1..360
                     /locus_tag="PLH57_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK02687.1"
                     /translation="ADVVTLHLPANKETKGLLSRERLSTLKDGAWLVNCARGGLVDEN
                     AVCDLLDSGKLRGAAFDVFREEPVQFPDRLAAHPKAIVTPHLGASTAEAQLRVGMTAA
                     DQITGFFLRGERAGVLN"
     gene            375..1667
                     /locus_tag="PLH57_08475"
     CDS             375..1667
                     /locus_tag="PLH57_08475"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRK02688.1"
                     /translation="MANVAILGAQWGDEGKGKVVDIYSEKSEFIVRYQGGNNAGHTLV
                     VNGEKTVLHLIPSGVLHEGKTCVIASGVVLDPAVFMEEIDELTKRGLLSEKRRTKVVI
                     SERAQVIFPYHKLLDRLREEHGGKGGKIGTTVRGIGPAYEDKVARRGILICDLYDPEL
                     LREKLERAVKEKNTVLEHLYGQPKLSIDELHQWAIEFGKKLKPMVCDTRVLVQEALAN
                     GKSILFEGAQGTLLDVEHGTYPYVTSSNTITGGIFTGCGVGPKSLEHIVGISKAYTTR
                     VGTGPFPTELADALGEQIRAKGAEFGATTGRPRRCGWLDLVALRYAVEVNGLTAIALM
                     KADVLSGISEIKVCTAYEIDGKRVNTLPANIRAFERAKPVYETLKGWGDLPSTAKGLS
                     DLPREFAAYVEYIERSLGVPVVTVSMGPGREQTLMRMM"
     gene            1744..1819
                     /locus_tag="PLH57_08480"
     tRNA            1744..1819
                     /locus_tag="PLH57_08480"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1777..1779,aa:Lys,seq:ttt)
BASE COUNT          567 a          543 c          615 g          477 t
ORIGIN      
        1 gccgacgttg tcactctcca tttacctgca aacaaagaga ctaaagggtt gttgagccgc
       61 gaacggttaa gcaccctgaa agacggtgct tggcttgtaa actgcgcgcg cggcggactg
      121 gtcgacgaaa acgcggtttg cgacctactc gattccggca agttacgcgg agctgctttc
      181 gacgtttttc gtgaagagcc cgtacagttt ccggaccgtt tggctgccca tcctaaagcg
      241 atcgttactc cacacctagg ggcatcgacc gctgaggcac agctgcgggt aggtatgact
      301 gcagcggacc aaataacagg ttttttcttg cgcggtgaac gcgcgggcgt tttgaactaa
      361 agaaaagagg atttatggca aatgtagcga ttctcggtgc tcaatggggt gatgaaggca
      421 aaggcaaggt ggtcgacata tattcggaga aatccgaatt tatagtgcgc tatcaggggg
      481 gcaacaacgc cggccacact cttgtagtga acggtgaaaa aaccgttttg cacctgattc
      541 catctggcgt tcttcacgaa gggaaaactt gtgtgatcgc aagcggcgtg gtcttggacc
      601 cagccgtttt tatggaagag atcgacgaac tcactaagcg cggcctactt agcgaaaaac
      661 gacgcactaa agtagtgatt agtgaacgag ctcaagtgat ttttccgtac cataaactac
      721 tagatcggct gcgcgaagag cacggtggca aaggcggcaa gattggtacg actgttcgtg
      781 gcattgggcc tgcgtacgaa gataaggtag ctcgacgcgg aattttgatt tgtgatttgt
      841 acgaccctga attgttgcgt gaaaagcttg aacgtgcggt gaaagaaaaa aacactgtcc
      901 ttgaacacct ctacggccaa cccaagcttt caatcgacga gctgcaccag tgggccattg
      961 aatttgggaa gaaattaaag cccatggttt gcgacactcg tgttttagtc caagaggcac
     1021 tggccaacgg aaaatcgatc ttgttcgagg gtgcgcaagg tacattgctc gacgtggaac
     1081 acggcaccta cccctacgta acctcgtcga atacgattac gggtggaata ttcacgggct
     1141 gtggagtggg accaaagagt ctcgagcaca tcgtgggtat ttcgaaggcc tatactactc
     1201 gtgttggcac cggtccgttt cctacggaac tggccgatgc tcttggcgaa caaattcgcg
     1261 caaagggcgc ggaatttggc gctaccacgg gtcgcccccg tcggtgcggg tggcttgacc
     1321 tagtggcgct ccgttacgct gtggaagtga atggtttaac ggcgatcgct ctcatgaagg
     1381 ccgatgtttt aagtggcatt tcggaaatta aagtttgtac ggcctacgaa atcgacggca
     1441 agcgcgtgaa cacgctacct gccaatattc gagccttcga gcgtgccaag ccggtttatg
     1501 aaactctaaa gggttggggc gacctgccgt cgaccgccaa gggtttgagt gaccttccca
     1561 gagagtttgc tgcttacgta gagtatatag agcgctcgtt gggagtgcct gtcgtaacgg
     1621 tgagcatggg tccaggccgc gagcagacat taatgagaat gatgtaacaa gagattgttg
     1681 acgacgcctc gccgggcggc tatacactca agttcctgtt tttgtaccga gattgtccat
     1741 tgggagcccg tagctcagtc ggtagagcat ttcactttta atgaaagggt cgacagttcg
     1801 attctgtccg ggctcaccac tcaaaaccca aagaaaacaa cccaaccccg aatcaacgcc
     1861 aactacgttt gtccaccaga cagaatcgaa cggaaatttg cggccgcagc ggctcgcaaa
     1921 gcgagtcgat gcggcaagtc gcacacgaag tgcgacttag caaattttcc atgccgagcc
     1981 ctgagcgcag gagcgcgaag gcgaaggccg attcagagat gagaccaacg caaggcacct
     2041 cacatccccg ccctaataaa cgagcgagtt tgagcgaagc gaaaacaagc ccgagcacaa
     2101 ttcagttccc gcccaagaaa accagcgagt ttgagcgaag cgaaaacaag cccgagcaca
     2161 attcagttcc cgccctaata aaccagcgag tttgagcgaa gc
//
LOCUS       DAOMXO010000409         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28979_length_2197_cov_5.252568, whole genome shotgun sequence.
ACCESSION   DAOMXO010000409 DAOMXO010000000
VERSION     DAOMXO010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28979_length_2197_cov_5.252568"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PLH57_08485"
     CDS             complement(<1..721)
                     /locus_tag="PLH57_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02689.1"
                     /translation="MHSDNLPNWNGSRESFYEVWYFKLNLVGPSGPGTKSPALWLRFT
                     TLSMQGGLKRVAEVWGIFFEPDAAGNVNKIAVKNTAKLSAPGMMENGKLSLGALQYSG
                     TGQVSIEDSVFGANFTRGSVVSRSSEISWDLSFEPNEFTFFHVPTALTKLKLSKSTVC
                     KPNVNVTFFGSFTVNGKRYECGTGAPGCQGHIWGTRYAHDWAWAHCNSFETKSGPAVI
                     EALTARVKLGGAIVSPQMSAMF"
     gene            complement(714..1184)
                     /locus_tag="PLH57_08490"
     CDS             complement(714..1184)
                     /locus_tag="PLH57_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02690.1"
                     /translation="MIKKSMIQYSLLMRVLGVVYLAAGAGFFFFPAETFYLINVGPKV
                     FKMFQDVPESTERFWLVLTFSMMMMLSFIAFYSSFQPKNYALALVHLLSKLISTLGFV
                     YVFFNSAPLFAYIVGAATDGTIFLVVLVATIRVMAAGSEKMVEEPTVETNTPHA"
     gene            complement(1709..>2197)
                     /locus_tag="PLH57_08495"
     CDS             complement(1709..>2197)
                     /locus_tag="PLH57_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF/VirB11 family protein"
                     /protein_id="HRK02691.1"
                     /translation="KTSLLNFLAQWIAPTERVITIEDTVELALNSTNHVQLQARVPNA
                     EGIGTVSLRDLLRCALRMRPDRIIVGECRGAEVLDMLQALNTGHPGSLTTVHANSPPE
                     ALHRLELLALLGAPNLSATSIARWIRQSVDWVVQVEREPSGQRRVALISEIVDNELRT
                     LG"
BASE COUNT          572 a          540 c          569 g          516 t
ORIGIN      
        1 aaaacatggc cgacatttga ggggatacaa tcgcgccgcc caactttact cgcgccgtaa
       61 gggcctcgat cacagctggc ccggatttgg tttcaaaact gttgcagtga gcccaggccc
      121 aatcgtgcgc atagcgagtg ccccagatat gtccctggca acctggcgca ccagtgccgc
      181 actcgtagcg ctttccgttg acagtaaaag aaccaaaaaa cgtgacgttc acgttcggtt
      241 tgcagacggt gcttttgcta agtttaagct tggtcagtgc ggttgggaca tgaaagaagg
      301 taaattcatt gggctcaaaa gaaaggtccc aggaaatctc cgaggaacgc gaaactaccg
      361 aaccgcgtgt aaagttggct ccaaatacgc tgtcttcaat cgaaacttga ccggttccgc
      421 tatattgaag cgcaccgagg ctaagcttgc cattttccat cattccaggt gcggaaagtt
      481 tggcggtgtt tttaacggct atcttattaa cgtttccggc ggcatcgggt tcaaaaaata
      541 taccccaaac ttccgcaact cgtttaaggc cgccctgcat tgatagagtc gtaaaacgca
      601 accaaagcgc gggggacttg gttcctggtc cgctagggcc gactaggttt agtttgaagt
      661 accaaacttc gtagaaactc tcacgggaac cgttccagtt cggtaaatta tcactatgca
      721 tgaggagtgt tggtctccac tgttggttcc tccaccattt tttcagaacc tgcggccatc
      781 acgcgtatgg tcgccactaa cacgactaga aaaatcgtgc cgtcggtggc agctcccacg
      841 atataagcga acaacggggc gctattgaaa aatacataaa caaaccccag ggtggagatc
      901 agtttcgaga gtaggtgcac tagcgctaaa gcatagtttt tgggttgaaa gctcgaataa
      961 aatgcaatga acgaaagcat catcatcatg ctgaatgtaa ggaccagcca gaatcgttcg
     1021 gtagactcag gaacgtcttg gaacatctta aaaactttag gtccaacatt gatcaaataa
     1081 aaagtttcag ccgggaaaaa gaaaaaacct gctccagcgg cgaggtacac gacgcccagt
     1141 actcgcatca gaagactgta ttgaatcata gattttttga tcatttgggt tctcgtaagg
     1201 ctccattgtt agattggggg acttgttcac aaacctatca gggaattttt gctcttccaa
     1261 gatttgggtc aaacaatatt cgtcgagatc cggggtccag ccgcccaaat agccgctccg
     1321 ataacgcgga gtgccttttg aagtgccgaa acacaataga gggtcgatgc accaaatatt
     1381 gggaatagtt ccttttgtcg ggaagtcgat cagaatattg cgtggcgaaa gatcgccgtg
     1441 tacgaagtcg ttttgccgcc aattaagctg cgttgtgcga aacatcgaat accagggcag
     1501 gccccgattt ctgaaatgta aaagtggcgt gccccgaacg tactgccaca caacacgagt
     1561 gagggtaaat tttcctactc gctgagctgt tcgttcgaac gattcaaggc gaggaacccc
     1621 caaaagggca cccgaacgaa gaactgcgat tcggcttgaa atttgaggct cgtctaggag
     1681 ccactcccat ttgacaagtt tttgggccct agcccaaggt tcgaagctca ttgtcgacaa
     1741 tctctgagat tagagccaca cgccgctgtc cgcttggttc gcgttcaacc tgtacgaccc
     1801 aatcgactga ttggcgaatc cagcgtgcaa tacttgtagc cgaaaggttg ggcgcgccga
     1861 gcagcgcgag tagctccagt cgatgaagtg cttccggggg cgaattggcg tgtacggtgg
     1921 ttagactacc tggatgccca gtgttgagcg cttgaagcat gtcgagcact tcagctccgc
     1981 gacattcacc gacaataatt cggtccggtc tcatgcgcag ggcacagcgc aaaagatcgc
     2041 gaagtgagac tgtgccaatg ccctccgcgt tggggactcg cgcttgaagc tgcacatggt
     2101 tcgtcgaatt gagcgcgagc tcgacggtgt cttcaatcgt gatcacgcgt tcggtcggag
     2161 caatccattg ggcgagaaaa ttcaacaagc ttgtttt
//
LOCUS       DAOMXO010000410         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_28985_length_2197_cov_4.215219, whole genome shotgun sequence.
ACCESSION   DAOMXO010000410 DAOMXO010000000
VERSION     DAOMXO010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28985_length_2197_cov_4.215219"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="PLH57_08500"
     CDS             complement(<1..1459)
                     /locus_tag="PLH57_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRK02692.1"
                     /translation="MNLDSKIPSGPLTQKWENHIANLKLVNPANKRKYTVIVVGTGLA
                     GASAAASLAELGYNVKAFFIHDSPRRAHSIAAQGGINAAKNYPNDGDTVWRLFYDTIK
                     GGDYRAREANVYRLAHLSTAIIDQCVAQGVPFAREYSGELANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQAMLRQVDAGKVTMYPRREMLDLVLKDGRAEGIVARDLVTGKIERHA
                     AHAVVLCTGGYGRVFYLSTNAMNSNATAAWRCHRKGAAFANPCFTQIHPTCIPVAGEH
                     AQSKLTLMSESLRNDGRVWVPKRKEDAKKHPSEIKDEDRDYYLERKYPSFGNLVPRDV
                     ASRNAKDVCDEGRGVGSSGLSVYLDFRDAIKRDGENVIREKYGNLFDMYHRITDENPY
                     QVPMRMYPAVHYTMGGLWVDYNLMSNIPGLHVLGEANFSDHGANRLGASALMQGLCDG
                     YFVIPYTIGHYLAQVGAFKLDSESSEFKAAEAAVNN"
     gene            complement(1456..2145)
                     /locus_tag="PLH57_08505"
     CDS             complement(1456..2145)
                     /locus_tag="PLH57_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRK02693.1"
                     /translation="MLPKIFSSSVFKKILMSLTGFGLVGFLITHLIGNANLYRSGSDP
                     FNTYAAKLHSLGPILEVAEIGLLGMFVLHIVTALQLKFRSRNARQDRYAVNASKKGPS
                     KSGFISQNMALSGLLILAFVVLHVKQLHFGPGMDAGYVTNVTGESTRDIHRLVVETFS
                     DPLNVALYVAAMIVLGLHLRHGFWSAFQSLGLTCERSSKALYIASGLLAALIAGGFLF
                     MPIWIYFGVQS"
BASE COUNT          587 a          572 c          520 g          518 t
ORIGIN      
        1 cgttgttcac cgcggcttcg gcagctttga actcggaact ttcggagtcg agcttaaaag
       61 cgccgacttg ggcaaggtag tgaccgatcg tataaggaat gacaaagtac ccgtcacaca
      121 acccctgcat gagtgcgctg gcgccaagtc gattagcacc atggtccgaa aaatttgctt
      181 cgccaagtac gtgaagcccg ggaatgttac tcatgagatt gtagtctacc cacaacccac
      241 ccatggtgta gtgtaccgct ggatacatgc gcatgggcac ctggtatggg ttttcgtcag
      301 tgatccggtg atacatgtca aagaggtttc catatttttc gcgaattacg ttttcgccat
      361 cacgtttaat ggcgtcacga aaatcaagat aaaccgaaag cccactactg ccgacaccac
      421 gaccttcatc gcacacgtct tttgcatttc gtgaggcaac atctcggggt accaagtttc
      481 cgaaacttgg gtatttacgc tctaaataat agtcccggtc ttcgtcttta atttcgctgg
      541 ggtgtttctt ggcgtcttcc ttgcgtttgg gcacccacac tcgcccgtcg ttacgcagac
      601 tttcgctcat aagagtgagc tttgactgcg catgttctcc cgctaccgga atgcacgtcg
      661 gatgaatctg cgtaaaacag ggattggcga aagctgcacc cttgcgatga cagcgccaag
      721 ccgccgttgc atttgaattc atggcattgg ttgaaaggta aaacacgcga ccatacccgc
      781 ccgtgcacag cactaccgca tgggccgcgt gccgttcaat tttgcccgtc accaaatcgc
      841 gcgcgacaat accttcggcg cgcccatctt ttaaaacaag atctaacatt tcgcgacggg
      901 ggtacattgt cactttaccg gcatcaacct gacgaagcat agcctggtac gcgccgagca
      961 aaagctgttg gcctgtttgg cctctagcat agaaggttcg cgaaacttgg gctccgccaa
     1021 acgaacgatt agccagttca ccgctgtatt cgcgagcaaa cggaactccc tgagccacgc
     1081 attggtcgat aatcgcagtg ctcagatgag ccaatcgata aacgttggct tcacgagcgc
     1141 gatagtcgcc gcctttaatt gtgtcgtaga acaaacgcca aacggtatcg ccgtcgtttg
     1201 gataattctt agccgcgttg atgccacctt gagccgcgat cgaatgggca cgtcggggag
     1261 agtcatgaat aaaaaaagct ttaacattgt agccaagctc cgccagcgaa gctgcggccg
     1321 aggcccccgc caaaccagtg ccgaccacga tcacagtata ctttcgtttg ttagctgggt
     1381 ttacgagttt taagttcgct atgtgatttt cccacttttg agtaagggga cctgacggaa
     1441 ttttagaatc taaattcatg attgcacccc gaagtaaatc caaattggca tgaacaaaaa
     1501 accacccgca ataagtgcgg cgagtagccc agaagcaatg tacagggcct tagagcttct
     1561 ttcgcaagtg agtcctaaag attgaaaggc gctccaaaaa ccgtggcgca gatgcaatcc
     1621 aagtacaatc atggctgcaa catagagcgc gacgtttaaa ggatccgaaa aagtttcaac
     1681 aaccaagcga tgaatgtctc gagtggactc gccagtgacg ttcgtcacat aacccgcgtc
     1741 cattccggga ccaaaatgta attgttttac gtgaaggacc acaaacgcca aaatcaaaag
     1801 cccactcaga gccatgttct gagaaataaa accacttttg ctagggccct tttttgaggc
     1861 attcacagcg tagcgatctt gacgcgcgtt ccgagaacga aactttaact gcaaggcagt
     1921 cactatgtgt aacacgaaca tgcccaacaa acctatttca gcgacctcta aaatgggtcc
     1981 gagactatgt aatttagctg cgtaggtatt aaaagggtcg ctgcccgacc ggtagaggtt
     2041 cgcgttgcca atcaggtggg taatcagaaa acctaccaac ccaaacccgg tcaaagacat
     2101 caaaattttc ttgaaaactg aggacgaaaa tattttcgga agcattcaca ctccagttgc
     2161 acaatttttg gatgcacctc ctaaattacc aagcggg
//
LOCUS       DAOMXO010000411         2190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29114_length_2190_cov_3.773302, whole genome shotgun sequence.
ACCESSION   DAOMXO010000411 DAOMXO010000000
VERSION     DAOMXO010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29114_length_2190_cov_3.773302"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLH57_08510"
     CDS             complement(<1..181)
                     /locus_tag="PLH57_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02694.1"
                     /translation="MALLRTFAVFTMMSVLLVRPPSAFANDSSPKSDGAKQCLQIVQA
                     SNTSDSSLAEADEIEI"
     gene            complement(243..845)
                     /locus_tag="PLH57_08515"
     CDS             complement(243..845)
                     /locus_tag="PLH57_08515"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRK02695.1"
                     /translation="MKKILSNALGTREAYQLLTSSIGPRPIAFVSTSNKNSVPNAAPF
                     CFFMGVTPSPPTIAFSVMRRGETKKDTIRNIENTRDFVVNIVDENLANAMNMASGSYP
                     PDMSEFDVTGLTPLKSELVTAPRIAESPIQLECKLKSILDLGDVQASIVIGEIVCFHF
                     KDGLLSDDGVVDIQKLRPIGRINENVYVRVTDFFEMNRPA"
     gene            complement(900..1169)
                     /locus_tag="PLH57_08520"
     CDS             complement(900..1169)
                     /locus_tag="PLH57_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02696.1"
                     /translation="MTNTNNTIRKAGALFGFAFALTLVLGAQSGFAGIANSDLKDFPT
                     VKDRKIAAEKQTVESNAKSESSATVARSETKTKRRPYFHRGVLAD"
     gene            1403..1858
                     /locus_tag="PLH57_08525"
     CDS             1403..1858
                     /locus_tag="PLH57_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02697.1"
                     /translation="MRAGCSSCGASRESFKTGGATAEAVVFCERCGSPNWFSEKLDYD
                     PFRASRHWRVGRVDRRAKMVVRAGCLLVGAIVLLSGMVLFYGAWLNMGGSAEGGGHDI
                     SRLWVSRLLAPGLLVVGIIFVLSAFRAPPAAPVIQLETERALRRGRSHG"
     gene            1941..2017
                     /locus_tag="PLH57_08530"
     tRNA            1941..2017
                     /locus_tag="PLH57_08530"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1975..1977,aa:Pro,seq:tgg)
BASE COUNT          482 a          484 c          641 g          583 t
ORIGIN      
        1 taatttcaat ttcgtcggct tccgcaaggg aagagtcgct tgtgttgctg gcctgtacaa
       61 tttgaaggca ttgtttggcg ccgtcggatt tgggcgaaga atcgttggca aacgcactcg
      121 gtggacgaac gagtagaacc gacatcattg tgaagactgc gaacgtgcgt aaaagagcca
      181 tatggagcgt gaatacgaga aagtgttcgt cgtgtctatt tttgattttt gacggctgct
      241 ttttacgcgg ggcggttcat ttcgaaaaaa tctgtgacgc ggacataaac attttcgttg
      301 atacgtccga tggggcgaag tttttggatg tcgaccacgc cgtcgtctga aagcaaaccg
      361 tctttgaaat gaaagcaaac aatttcgcca atgacgatcg atgcttgcac atcaccgaga
      421 tcgagaattg atttcagttt gcattcgagc tgaatgggac tttcggctat gcgcggtgcg
      481 gtaaccaatt cgctttttag cggtgtgagc cctgtgacat cgaattcgct catgtcgggc
      541 ggataactgc cggacgccat gttcatggcg tttgcgaggt tttcgtcgac aatattgacc
      601 acgaagtctc gagtattttc gatgttgcgg atcgtatctt ttttggtttc gccgcgacgc
      661 ataacactaa acgcaatcgt gggcggggaa ggtgtaactc ccataaaaaa acaaaaaggc
      721 gccgcattgg gaactgagtt tttgttggaa gtagagacga acgcgatggg tcgcggacca
      781 atacttgaag tgaggagctg gtaggcctcc cgcgtgccca acgcgtttga aagaattttc
      841 ttcatgctcc ctccaatagt cttttaaaga ctattggagt ttgcagcgcc cggcaaggcc
      901 tagtcagcta acacgccgcg atgaaaatag ggccgtcttt tggtctttgt ctcggatctt
      961 gcgacggtag ccgacgactc agatttggcg ttcgattcaa cagtttgttt ttcggcggcg
     1021 attttacggt ctttcaccgt tggaaaatct tttaagtcgc tgttggcgat tcccgcgaac
     1081 cctgattgag cacccaaaac gagagttaga gcaaatgcga atccaaaaag cgctccggcc
     1141 ttgcggattg tattgttagt gtttgtcata aaaagagacc tccatatttc ttctgacgta
     1201 ttgccgggaa ttttcttaca gcgcgtgtgg gaggctgaag cctaagcgca gacccccgtg
     1261 ggacgcgggg aggggggaga acccttctgg ttcaaggccc gatcctgtat aaaagtgtag
     1321 ggccgaaccc aaggggcgtc aagttggtgg gcggcaagat tttgccggcg acttcgtgta
     1381 gactgaggct gggagggctt tcatgagagc aggatgctcc agttgtggtg cctcgagaga
     1441 atcatttaaa acgggcggag ctaccgccga agcggtagtg ttttgcgaac gttgtggttc
     1501 gccaaattgg ttttcagaaa aattggatta cgaccctttc cgggcaagtc gtcactggcg
     1561 ggttggacgg gttgatcggc gcgctaagat ggtggtgcgg gccggttgtc ttttggttgg
     1621 ggccattgtg ttgcttagcg gaatggttct attttacggg gcctggctaa atatgggggg
     1681 ctcggccgag ggcggcggcc acgatatttc gcggctttgg gtgtcgcgtt tgttggcacc
     1741 cggtttactc gttgtaggga tcatattcgt tttaagtgct tttcgggctc ctccggcagc
     1801 ccccgtgatt cagttagaga ctgaacgagc ccttcgtcga ggtcggtctc acggttaagc
     1861 tttgcaattt tcaagatttt tgctaattct tgctcctcgt ttttaagccg atcaagagac
     1921 cagggttcgg ttcagtcagt cggggtgtag cgcagcctgg tagcgcactt ggtttgggac
     1981 caagttgtcg caggttcaaa tcctgtcacc ccgaccattt aaaaaagccc gccatgctaa
     2041 tgcatggtgg gcttttttat ttgtctgagc gacagatttc gaagcaagcc ccgaacgaag
     2101 tgaggagcag gttcttcaaa aaagccatga aggctttttt gcacgagcac ggccaaaggc
     2161 cgcgcgcagc ccgaagggca gctggcaaga
//
LOCUS       DAOMXO010000412         2186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29190_length_2186_cov_6.088221, whole genome shotgun sequence.
ACCESSION   DAOMXO010000412 DAOMXO010000000
VERSION     DAOMXO010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29190_length_2186_cov_6.088221"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..645)
                     /locus_tag="PLH57_08535"
     CDS             complement(25..645)
                     /locus_tag="PLH57_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02698.1"
                     /translation="MTRWIEVMPSFYQTNYQGENNMERHEGNKLEVQTKRAWTRAEAK
                     AIWKANFRELLLIGMFAGLGAAGLLAVMFVTTARAALAPGASVVQEQVFEFQANGSAA
                     KKALGDLQIRLIENGSRAVRGSIWSIQSKSRKILANCVEEKIERFSAPPSGPKLVHFE
                     CTDASLGTMRMPLIIVWSNANAPNVARASLGSALFGNKLQGSLKKL"
     gene            835..1158
                     /locus_tag="PLH57_08540"
     CDS             835..1158
                     /locus_tag="PLH57_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRK02699.1"
                     /translation="MNMTKTEIEKHAPEVSRTLKLLAHPIRLKLLCALSMGEKTVTEL
                     VQYVEASQSWVSQFLTRMRLEGLVDVRRDSKFAYYRIRDVRILSIMEVIQTQYCKPTR
                     RKNHD"
     gene            1151..1702
                     /locus_tag="PLH57_08545"
     CDS             1151..1702
                     /locus_tag="PLH57_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRK02700.1"
                     /translation="MTEVILDVRERDEFEAEHVPGSISVPLSHLDSVAPGVLDQLKER
                     NVTLMCRSGARAKLAYEGIQRLGFGDKVALTVFDGGILEWKKQGKPTTVRKRGHLPIM
                     RQVQLAAGLMILASVAAGFWLAPAFFGVTVFVGAGLTVAGSTGFCGMANLLALMPWNK
                     AVDSTKEELCAVSPSSQTCKEEE"
     gene            1706..>2186
                     /locus_tag="PLH57_08550"
     CDS             1706..>2186
                     /locus_tag="PLH57_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02701.1"
                     /translation="MKTIVKELSEIMTDGFKIPAPWRARVLVNDDNMTYLVWNENTRE
                     GLFVDPVAGDLDLQLSEAAKIPNTRWIAVIDTHTHADHISNAGELAAKLTAPLIMHAN
                     APTKLVHLRVSRDGLLPTAGGPLRFLVTPGHTHDSITPIWGPFIFGGDTLLYGETGRD
                     "
BASE COUNT          533 a          585 c          545 g          523 t
ORIGIN      
        1 ttgtttcgaa gacgtctgga ataatcagag tttttttaac gagccctgga gtttgttgcc
       61 aaacaaagcg ctccctagac ttgctcgcgc aacattgggg gcgttggcgt tactccacac
      121 gatgataagc ggcatgcgca tcgtacccaa cgaagcgtcc gtgcattcga agtgtacgag
      181 tttgggacca ctgggtggcg cagaaaatct ttcaattttc tcttccacac agttcgccaa
      241 aatcttccgt gattttgact gtatgctcca gatcgagccg cgcaccgcac gactgccgtt
      301 ttcgatcaga cgaatctgaa gatcccccag tgcttttttg gccgcactcc cattcgcctg
      361 gaactcgaaa acttgctcct gcaccacact cgcaccagga gcgagtgcag ctcgcgcagt
      421 ggttacgaac attaccgcaa gcaggcctgc agcgccgagg cctgcgaaca tcccgattaa
      481 caataattct cgaaaattgg ccttccaaat ggcctttgct tcagcgcgcg tccatgcacg
      541 cttggtctgc acctcgagtt tattgccttc atggcgttcc atattattct ctccctgata
      601 atttgtttga taaaaactcg gcatcacttc aatccatcga gtcattctgc gcctcctccg
      661 tcgaggccgt actctcctat cgcaaggtta gtgccaacca ctgctgggcg gcgcgctcga
      721 gggaaaaagg ggacaagggg ggctttacca gtaaattcag ggcttttttg gcaccagagc
      781 gaggggccat tttggccact tgacatatta taaaataata atattataat atgtatgaac
      841 atgaccaaaa ctgaaattga aaaacacgcc cccgaagtca gccgaacact taagctcctt
      901 gcccacccga ttcgactcaa acttctgtgt gcgctttcaa tgggtgaaaa aacggtaacc
      961 gagcttgtgc aatacgttga agcgtcccaa tcctgggtgt cgcaatttct cacccgcatg
     1021 cgtctggagg ggctcgtcga tgttcgccgc gattcgaaat tcgcctacta tcgaattcgc
     1081 gatgtgagaa ttttaagcat tatggaagtg attcagaccc aatattgtaa accaaccagg
     1141 aggaagaacc atgactgaag ttatattaga tgtacgcgag cgagacgaat tcgaggccga
     1201 acatgtaccg gggtccatta gtgtaccctt atcgcatctg gattcagtgg ctcccggcgt
     1261 cttagaccaa ttgaaagaac gaaacgttac gctcatgtgt cgttccgggg ctcgcgccaa
     1321 actggcatac gaaggtattc aacgactcgg gtttggcgac aaagtcgcac tcaccgtttt
     1381 cgacggcggt attttggagt ggaaaaaaca gggcaaaccc accacagtgc gaaagcgagg
     1441 tcatctccca atcatgcggc aagttcagtt ggccgcggga ctcatgattc ttgcgagtgt
     1501 cgcggctggt ttctggctcg cacccgcgtt ctttggcgta acggtgtttg tcggcgctgg
     1561 actcaccgtg gccggaagta cggggttctg cggaatggcc aatttgctgg cactcatgcc
     1621 gtggaacaaa gcagttgata gcaccaaaga agaactatgc gctgtgagcc ccagctctca
     1681 gacctgtaaa gaagaggagt gaactatgaa aacaatcgta aaagaacttt ccgaaataat
     1741 gaccgatggt tttaaaattc cggctccctg gcgtgctcgg gtactcgtta acgacgacaa
     1801 catgacctat ctcgtttgga acgaaaacac ccgcgaggga ttgttcgtgg atccggtcgc
     1861 cggggacctc gaccttcagc tcagcgaggc tgctaaaatt ccaaacactc gctggatcgc
     1921 cgtgatcgac acccacaccc acgcagatca catttcgaac gctggtgaat tggctgcaaa
     1981 gctaactgct ccgcttatca tgcacgcgaa tgcgccaacc aaacttgtac atctgcgggt
     2041 gagtcgcgat ggactgcttc cgacagcagg tggcccactt cgttttttag tcacccccgg
     2101 tcatactcac gattcgatca caccgatttg ggggcccttt atattcggcg gagacaccct
     2161 cctgtacggt gaaactggtc gcgacg
//
LOCUS       DAOMXO010000413         2186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29199_length_2186_cov_3.062412, whole genome shotgun sequence.
ACCESSION   DAOMXO010000413 DAOMXO010000000
VERSION     DAOMXO010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29199_length_2186_cov_3.062412"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PLH57_08555"
     CDS             complement(<1..1065)
                     /locus_tag="PLH57_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRK02702.1"
                     /translation="MKILLTGATGYIGGRLVPKLLEQGHEVRVLVRDPNRIAGRPWTS
                     RVQIFTGDLVQNKGLVPALDGVDAAYYLVHSMLSGPDFQNLDHKAAENFAAAAKHVKH
                     VIYLGGLVPKAGAISEHLKSRAEVGDILRKALPTTEFRAGPIIGSGSASFEMLRYLTE
                     RLPIMIAPRWIKNEIQPLAVRDVLGYLVAALNVAPSGVIDVGADRLTFKKMMQVFAEV
                     RGLRRTILPVPVFAPGLASRWVGLVTPIPNSIAVPIIEGIIQPLIADTAQAERLFPDI
                     KPIPYRKAVELSLARLQSGGTETRWSNALGQDSNASYLVKNEEGMIREVRRLKVNASP
                     ESLFKVFSQLGGEKGWLAWRW"
     gene            1203..1619
                     /locus_tag="PLH57_08560"
     CDS             1203..1619
                     /locus_tag="PLH57_08560"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRK02703.1"
                     /translation="MKILFMCVANSARSQLAEGLARKLFPDAEIQSAGSQPGKLNPYA
                     VAVMNELGIDISSHYSKSLDDLSTEFIQNLDYVITLCAEEVCPTLVSKAKKIHWPHPD
                     PASKTELPESEALQRFRVARDAIQQKLKTFKSELAL"
     gene            1616..1741
                     /locus_tag="PLH57_08565"
                     /pseudo
     CDS             1616..1741
                     /locus_tag="PLH57_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019817018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
     gene            1879..>2186
                     /locus_tag="PLH57_08570"
     CDS             1879..>2186
                     /locus_tag="PLH57_08570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRK02704.1"
                     /translation="MFIRSRFFVLLFSASTALLFTSNAVAGARSHVKRFVKTRVLTML
                     PANVLIVRGDPNSKKVALTFDDGPDEMTPKYVEVLESLNVKATFFLLGKQIEKFSYYA
                     "
BASE COUNT          529 a          528 c          563 g          566 t
ORIGIN      
        1 ccaacgccaa gcgagccagc ctttttcacc accgagttgg ctgaagactt taaataaact
       61 ctcgggcgag gcattcactt tcaagcgacg tacttcgcga atcatgcctt cttcgttttt
      121 gactaaataa gatgcattgc tgtcttggcc taaagcgttg ctccaacggg tttcggtacc
      181 accgctttgc aagcgcgcaa gagaaagctc gacggctttg cggtaaggaa taggtttaat
      241 atcgggaaac aagcgttcgg cttgggcagt gtcggcgatg agtggctgaa taatgccttc
      301 gataattggc accgctatgg aattcggaat cggagtcact aaccccaccc agcgtgatgc
      361 aaggccaggg gcgaacaccg gcaccggcaa aatcgtgcgc cgcagtccgc gtacttcggc
      421 aaatacttgc atcatttttt taaaggtgag tcgatcggcc ccaacgtcga tcacgccgct
      481 tggagccacg ttgagtgcgg ccactaaata accgagtacg tcgcgcacgg cgagaggttg
      541 aatttcgttt ttgatccagc gtggggcgat catgattggg agccgttcgg tgaggtagcg
      601 aagcatttca aacgacgccg aaccggaacc gataattgga ccagctcgaa actcggtggt
      661 gggtaaagcc ttgcgtaaaa tgtcgccaac ttcggcacga ctttttaaat gctccgatat
      721 ggcgccagcc ttgggcacca gaccgcccaa atagattacg tgtttgacgt gctttgcagc
      781 ggcggcaaaa ttttcggccg ctttgtggtc gagattttga aaatctggcc cagagagcat
      841 tgaatgaacc aggtaataag ccgcatcgac gccatcgagc gcaggaacca aacctttgtt
      901 ctgcaccagg tcgccggtga agatttgaac tctcgaagtc caaggtcgac cggcgatgcg
      961 gttagggtcg cgcaccagca cgcgcacctc gtggccttgc tccagaagtt taggaacaag
     1021 tcgtccgccg atatagccgg ttgcgccggt gagtagtatt ttcatcttgg ctgtcattat
     1081 atgccgtctt ttaaaagagc cataaatttt ttgggttcat tgttgggata tttctcgttg
     1141 agatatttaa gatttggttt agaggctttc ttgtgcccag agttcttgtt agaacaaaaa
     1201 ccatgaaaat actttttatg tgtgttgcca attccgcccg cagtcagctg gcggagggtc
     1261 tagctcgcaa gctgtttcca gacgccgaaa ttcaaagcgc tggctcgcaa ccgggaaaac
     1321 taaatcccta tgccgtggcg gttatgaacg agcttggaat tgatatttcc agccattatt
     1381 cgaaatcgct agacgatctt tcaactgaat ttattcaaaa cctcgactat gtgattaccc
     1441 tttgcgcgga agaagtgtgt cccacactag tgtcgaaggc caaaaaaatt cattggccgc
     1501 accccgatcc ggcgagcaaa accgagttgc cagagtctga ggccctgcaa cggtttagag
     1561 tcgctcgcga tgccattcag caaaaactca aaacttttaa aagtgagctt gcgttatgat
     1621 tcggtcacga gtgtttattg gcgaattcct gggaacggcg tttcttttaa tggttgtcgt
     1681 tggttcagga atcatgggcg aaagtctggc tcagggcaat gcagcgatcg cgcatttggc
     1741 cgcccttgcc ccgactttta atcgacttct aagttggggg gcatcaaatc atgcgtatta
     1801 tcgagtgttt acgtcggcca aaaggattgg gcaaaccccc gatagtggat tctacgattt
     1861 agtgctataa ggccgcacat gtttattaga agtcgttttt tcgtgctctt gttttcggcc
     1921 tctaccgcac tgctttttac gagcaatgct gtcgcaggtg ctcggtctca cgttaaacgg
     1981 tttgttaaaa ctcgggtcct aactatgctt cccgcaaatg ttttgatcgt gcgcggagac
     2041 ccgaactcca aaaaagtggc gctcactttt gatgatggcc ccgacgagat gacgcccaaa
     2101 tacgtagaag tgcttgaatc gctcaatgtg aaagcaacat tttttctact gggcaaacag
     2161 atcgaaaagt tttcgtacta cgccca
//
LOCUS       DAOMXO010000414         2178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29366_length_2178_cov_4.477155, whole genome shotgun sequence.
ACCESSION   DAOMXO010000414 DAOMXO010000000
VERSION     DAOMXO010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29366_length_2178_cov_4.477155"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..775)
                     /locus_tag="PLH57_08575"
     CDS             complement(98..775)
                     /locus_tag="PLH57_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRK02705.1"
                     /translation="MKARILVVEDETDIRDLMLLHLKRDGYEVDSVDNGEEALKKIEN
                     ENYSLIVLDWMLPGISGLDVCKKIQNKAIPVLMVTARADTADIVVGLEMGADDYITKP
                     FELPIFNARVRALLRRLLLPRNLMNAQTFEVGALRVRVDAHEVLCDNNPIQLTTSEFK
                     LLVALLANQGKVLSRSKLVEMVQGEGVSVVDRTVDTHVFGLRKKLGPCAEVIETIRGV
                     GYRVQSN"
     gene            complement(778..1455)
                     /gene="phoU"
                     /locus_tag="PLH57_08580"
     CDS             complement(778..1455)
                     /gene="phoU"
                     /locus_tag="PLH57_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRK02706.1"
                     /translation="MERHFDIELRSLKNELIKMAGFVEQSVAYAVEAIRAQDPNIAQT
                     KFKQIFEVERKVNQAHKDVDDACVKLLALQQPLAADLRLIVAIIKMNSDLERMGDQAV
                     NIAHNSERFLKLEPKQTMEDLVVMGDEVRTMVRGALDCFINRDGEMARKILALDDSID
                     EARNRIFSEMKTYLKTHPENIEHGFNVIFIARNLERIGDHATNIAEDVVFAITGEDIR
                     HGGSKGV"
     gene            complement(1463..>2178)
                     /gene="pstB"
                     /locus_tag="PLH57_08585"
     CDS             complement(1463..>2178)
                     /gene="pstB"
                     /locus_tag="PLH57_08585"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRK02707.1"
                     /translation="DLDAVKGIDLRFEKNSVTAIIGPSGCGKSTYIRCLNRLHEEVPG
                     AYSSGEVLLNGENIQGLRVDPVAIRRRIGMVFQKPNPFPSLSIFDNVCVGLKLSGVRK
                     SSVLKEVVETSLRAAALWDEVKDKLDAPGVSLSGGQQQRLCIARTLAVEPTVLLMDEP
                     TSALDPISTAKIEELVTDLKKRVTIVIVTHNMQQAARISDRTAFFLLGDLIEFDDTSR
                     LFTAPKQEQTERYITGRFG"
BASE COUNT          568 a          562 c          443 g          605 t
ORIGIN      
        1 ataacctact ccgcgtatgg tttcgatcac ctcggcacag ggacctaatt tttttgaacc
       61 gcgagatgag gcgccaaggc agatgattca tagatattta gttactctga acgcgataac
      121 ctactccgcg tatggtttcg atcacctcgg cacagggacc taattttttt cgcaaaccaa
      181 aaacatgagt atccactgtg cgatcgacaa cactgactcc ttctccctga accatttcta
      241 ccaatttcga ccttgaaaga actttccctt ggtttgcaag caaggcgaca agcaacttaa
      301 actctgacgt tgtcagctga atcggattgt tgtcacaaag aacttcgtgg gcgtcaaccc
      361 gcacgcggag agctccaact tcaaaagtct gggcgttcat taaatttcgc ggcagtagca
      421 gtcttcgcag taaggctcgc actcgcgcgt taaaaattgg tagctcaaat ggtttggtaa
      481 tgtagtcgtc ggcacccatt tcaaggccga ccacaatatc ggcggtatcg gcccttgcgg
      541 taaccataag tacaggaatt gctttatttt gaatcttctt acatacgtca agacccgaaa
      601 tcccaggcaa catccaatcg agcacgatta acgaataatt ctcattttct atttttttta
      661 gtgcctcttc gccgttatcg accgaatcga cttcataacc atctcgcttt aaatgcagca
      721 gcattaagtc gcgaatatcc gtttcgtctt cgactaccaa aattctagcc ttcatcacta
      781 aactcctttg gagcccccgt gccgaatatc ttcgccggtt atggcaaaca ctacgtcttc
      841 cgcaatattt gtggcatgat ctccaatccg ttccaaattt cgagcaataa aaataacgtt
      901 aaaaccgtgc tcgatatttt caggatgggt ttttagataa gttttcattt cagaaaaaat
      961 tctatttcga gcctcgtcta tcgaatcatc aagtgccaaa atttttcggg ccatttcgcc
     1021 gtcgcggttg atgaaacagt cgagcgctcc gcgcaccatg gtgcgcactt cgtcacccat
     1081 aaccacgaga tcttccatag tttgttttgg ttctaacttc aaaaagcgtt cagagttatg
     1141 agcaatattt actgcctgat cgcccattcg ctccaaatca gaattcattt taatgatcgc
     1201 tacaattagg cgaagatcag ccgccaatgg ctgctgaagt gccagtaatt ttacgcaagc
     1261 atcgtccacg tctttgtggg cttgatttac ttttctttcg acttcgaaaa tttgcttaaa
     1321 tttagtttgg gcaatattgg ggtcctgcgc tcgaatcgct tccaccgcgt aggcgacaga
     1381 ctgttcgaca aaacctgcca tcttaatcag ttcattttta aggcttcgaa gttcaatatc
     1441 aaaatgccgt tccataggac gatcaaccaa accttccggt aatataacgt tctgtttgtt
     1501 cttgtttcgg tgccgtgaac aagcgcgagg tatcgtcgaa ttcgattaaa tcaccgagca
     1561 gaaaaaacgc tgtccgatcc gaaatacgcg cagcttgctg catattgtgc gtcacaatga
     1621 caattgtcac acgtttcttt aagtctgtca caagctcctc gatcttggca gtagagatcg
     1681 gatcaagcgc acttgtaggt tcgtccatca gtaaaaccgt aggttctacg gccaacgtac
     1741 gggcaatgca cagccgctgc tgttgcccgc cagaaagact tacgcccggc gcatctagct
     1801 tgtctttgac ttcgtcccaa agtgcggctg ctcgtagact ggtctccacg acttccttca
     1861 acacactaga ttttcgtaca cccgacagtt tcagccctac acacacatta tcgaaaatag
     1921 agagcgaagg gaacggattg ggtttttgaa aaaccatgcc gatccgccgg cgaatcgcca
     1981 ccggatcaac gcgcagccct tgaatgtttt cgccgtttaa caacacttcc ccagaagaat
     2041 aggctccggg cacttcttca tgcagtcgat ttaaacatct gatgtaggtg gattttccac
     2101 accccgacgg tccaataatg gcagtaactg aatttttttc aaatcgaaga tcgattccct
     2161 taaccgcatc gagatcgc
//
LOCUS       DAOMXO010000415         2170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29508_length_2170_cov_8.523877, whole genome shotgun sequence.
ACCESSION   DAOMXO010000415 DAOMXO010000000
VERSION     DAOMXO010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29508_length_2170_cov_8.523877"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..1297
                     /locus_tag="PLH57_08590"
     CDS             479..1297
                     /locus_tag="PLH57_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02708.1"
                     /translation="MSRYLNKTLALAALCITLGITNSLGQSKLTNRNNRELSTVFSDL
                     ANFHSATYEQSLRALFSLPPSQFDSVLNAVTSSNLDESDGAQRLIRYLGDRELLSKLV
                     ENCKTRCLLSGPVPIPLHPLDYREIEYLTRAGDVCKWGEFDMRYVFALFLDTTQPALE
                     LRRRVENGSRQCASNAILTSTIRTIHDTKGLETAFTLQNSVEAVRSELFFLTEVERER
                     AKIQFLGFNFQKNRALFEVLVNNGILASRYVHVVFHKVDSKNWQFYSITVVNQS"
     gene            1486..1875
                     /locus_tag="PLH57_08595"
     CDS             1486..1875
                     /locus_tag="PLH57_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02709.1"
                     /translation="MKLAFKLIFGLIYTVVYVVLAVDSTGGGHGAFIFFAPLFTWPLI
                     LMALGLTTRVNNSIVRILVPMLLIVSYISIGAMFLDECIHGDGSVQRRWEMSPYFVIL
                     ILGWYFAGQLIIWSTYILELRRQNRLD"
BASE COUNT          564 a          515 c          582 g          509 t
ORIGIN      
        1 tgacacagaa agaaagcaga aggaaaagcg ctcgatccgg caaaactgcg atattctgct
       61 taagtcccag aaaccggcga aacaaccgtc ttcgcgtatg atgcttcaaa caggcgggtt
      121 gccgagtatt caaccatcgt cgaaccggcc gcaaccgcca aaaccagcta tttgacaacc
      181 gatcatcttg ggagcccgcg ggtggtgacg gacgaggacg ggcgggtgac gagccggcgg
      241 gactacactg cctttggcga ggagagcgtt acgccggagc gggcggcggg gcttggctat
      301 gcgggccaga gcgagacgcg gaagggctac accggctacg agaaggataa tgaaagcgga
      361 ctcgaattcg cccaggcgag gtactacaac gcgacccacg gccaattgag ccgattccgg
      421 ttcggaggac actgatttgg caacgatctg tgggcgtcgt gaggaggtag caatacacat
      481 gagtcggtat ctcaacaaaa cgttagctct ggcagctctc tgtattaccc tgggaataac
      541 gaattcgctg gggcaaagta aattaacaaa caggaataat cgagaactat caacggtttt
      601 ttcggatctg gcgaactttc attcggcaac ctatgagcaa tcgttgcgcg cacttttttc
      661 gttgccgccg agtcaattcg acagtgtgtt gaatgcggtg acgagctcga acctcgacga
      721 atccgatggg gctcagcgtt taattagata tctcggcgac cgtgaacttc tgagtaaact
      781 agtcgaaaat tgcaaaacgc gctgcttgct ttctggcccg gtgccaattc cgcttcatcc
      841 gctagactat cgtgaaatcg agtatctgac gagagcaggc gacgtctgta aatggggtga
      901 attcgatatg agatacgtat ttgctctttt tctcgacacc acacagccag ctctagagtt
      961 gcggaggcga gtagaaaacg gctcccgcca atgtgcatca aacgcgatat tgacatcaac
     1021 gatccgaaca attcacgaca caaaaggact tgaaaccgcg tttactttac agaattcagt
     1081 tgaagcagtc cgatccgagc tgtttttctt aaccgaagtc gaaagggaac gtgcgaagat
     1141 tcaatttcta ggtttcaatt tccaaaagaa tcgcgcgctg ttcgaagttc tagtaaacaa
     1201 cggaattctc gcaagtcgtt acgtccatgt tgtttttcac aaggtcgatt cgaagaattg
     1261 gcaattctat tcgatcaccg tggtgaacca gtcgtaatga gcacctgatc ctcttttgaa
     1321 ttgccgcaaa acagcgaacc aaggctactg gaactatgcc ggcagcttca ccgaaaacgc
     1381 ggcggtgcgg tttcgagcac gaagctcggg aacggaaaaa tgagaaaaca aaaactaccg
     1441 tgaaagtaat tgaggaggaa taagcgggtc gtttgggagg ttgacgtgaa attagcattt
     1501 aagttaatct tcggcttaat ttacaccgtt gtgtacgttg ttctcgcagt tgatagtaca
     1561 ggtggcggac atggagcttt tatttttttt gcaccacttt tcacctggcc cttgattctg
     1621 atggccctcg ggcttaccac cagagtcaat aactcgatag tgcgcatatt ggtaccaatg
     1681 ctgctaatcg tttcatacat cagtattggc gcaatgtttc tggacgagtg cattcacggt
     1741 gatggttcgg tccaacgccg ttgggaaatg tcgccttatt tcgtaatact aattcttggc
     1801 tggtacttcg cgggtcagtt aattatttgg agcacgtaca ttctcgagtt gcggcggcaa
     1861 aaccggttag attgaatttt acattccaaa gatgggccaa ggagcagtct cttacacaac
     1921 ttcgcccgag cattggtcga gcagacctat ccctcaggcc ggaagttgaa gaacgtgctc
     1981 gacgcgagcg gtgatctctc gatggttcag agtgcccggt gcatggacgc cgcggcgggc
     2041 acggacgccg actgcgccgc acaggccggc ctctggaact acgcaaagaa tttcacctac
     2101 aacgcggctg gtgctgtgac gagcatgcaa ctgggcaatg gaagatggga atctacgcaa
     2161 tttaattcaa
//
LOCUS       DAOMXO010000416         2170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29516_length_2170_cov_4.486525, whole genome shotgun sequence.
ACCESSION   DAOMXO010000416 DAOMXO010000000
VERSION     DAOMXO010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29516_length_2170_cov_4.486525"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PLH57_08600"
     CDS             <1..729
                     /locus_tag="PLH57_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02710.1"
                     /translation="ASTCGDNLVSSTSKITRIPSLRVVPNIFFQADPEFDSDKKIEEL
                     KLNTSEPFLSDTFLISGPAYRFHAGMGIINDGSGDIENVVLVVFIRDPAVFVIGSIGG
                     SIFLGNTFLFNQSPAMIDKNEPVFGGDYLNPDPYPGYVGYVALGPLRAGDGLVFDVKM
                     GVGPGTLMHFEAVGFINENTWAVPVGAVHLEGNNPVSGTSVRIASPELNSKVERYFEI
                     PAVQVGEPSKSVVETLQKTRAVSE"
     gene            1277..>2170
                     /locus_tag="PLH57_08605"
     CDS             1277..>2170
                     /locus_tag="PLH57_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02711.1"
                     /translation="MRTQFWLPDKVEAIKAIDGLIPGNQLQAVALKRLNASLAQLWDN
                     ERRLTTEAFKRLNRNRLQALALRLHSTELSLARINSQFQQIQSELSKIEAAEQATSSA
                     HQQWRKTANGFESLYAEVQNLHSKIKQQLDEQNENNKKCIEKFKANTKAISRLAEHVD
                     TSIVLPPSVVSAIQKSQESAGDTSKFLNESTDTMNQAYDEIKKIDEKAKQVSDALAKL
                     ETDKMLVIMIDNGRLKSATYESEKDQQQYEQIKRNIEVLRKQREELVRLNIAAKTPTE
                     AAANMRKIADLDFKIQQAQDST"
BASE COUNT          660 a          488 c          486 g          536 t
ORIGIN      
        1 gctagtacgt gtggcgacaa tctagtgtct tcgacttcca aaattacccg cataccttcg
       61 ctgagggttg tacccaatat tttttttcaa gccgacccag agtttgattc cgataaaaaa
      121 attgaagagc tgaaattaaa cacatccgag cctttcttga gcgatacttt tttaatttca
      181 gggcccgcat atcgatttca tgccggaatg ggcattataa acgatggatc gggcgatata
      241 gaaaatgtag tgctcgtcgt ttttattcgg gaccctgccg ttttcgtgat tggatcgatc
      301 ggcggatcaa tatttttagg caatacgttt ttatttaatc agtctcctgc aatgatcgac
      361 aagaacgaac ccgtttttgg aggagattac ctaaatcccg acccctaccc aggttacgtg
      421 ggttatgtgg cacttgggcc gcttcgtgcg ggcgacggtt tagttttcga cgtcaaaatg
      481 ggagttgggc caggtacctt aatgcatttt gaggccgtcg gctttattaa tgaaaatact
      541 tgggccgtgc cggtcggagc tgttcatttg gagggaaaca acccggtgag tggcacgagt
      601 gtcagaattg ccagtcccga gctaaactct aaggtggagc ggtactttga aattccggcc
      661 gttcaagttg gcgaaccgtc aaagtccgtg gttgagacgc tgcaaaaaac tagggccgta
      721 tctgagtgaa aattctcgtc tatagggcgg agagggtggg gaacataaaa tccgtgaata
      781 tcaacgcctg ctttgcgagc gtagatcatc atggtttcga tatccctcca gccccccatc
      841 ttcggcatac ccctaccgat cggccaaacc ggagaacaaa tcgtgattgg cggattcgcg
      901 ggctccgctg taattccaag ttgttagggt gccacggaac cactaaattt ggctttgtta
      961 caaatctgaa gctttaaaag ggtatcgaat tcgtggagat ttagaacacg cctcgcaatc
     1021 gtctggccta ccagacaatc cattacgcaa ttccagaatt caatacgcac catataccag
     1081 cgcatactgt gtactcgcat tggcaccgcg tttgctttgt ccaattgcaa tgatacttct
     1141 acaaacgcta ttatacatat tggttagtat tgtgtttaat cgcgacgtac tcgcggcaaa
     1201 caatttcgac gaagcaaaaa acgcgggcca ttttgtaata agcactagaa gcgaaaattg
     1261 gtcaaatcgc tctgaaatgc gaacacaatt ctggttgccc gataaagttg aagcaattaa
     1321 agcgatcgac gggctaattc ctggcaatca acttcaagcg gtggcactaa aacgtctgaa
     1381 tgcttcttta gcacagctct gggataacga acgcagactg accactgaag cattcaaacg
     1441 gctcaatcgc aatcgtctgc aagccttagc gctgcgttta cacagcaccg aactgagtct
     1501 tgcacgaata aattcacagt ttcaacagat tcaatccgaa ctcagtaaaa tcgaagctgc
     1561 agagcaggcc acctctagcg cgcaccaaca atggcgcaaa acagcaaatg gttttgagtc
     1621 gctatacgcg gaagtgcaga atttacacag caaaattaaa cagcagctcg acgaacaaaa
     1681 tgaaaataac aaaaagtgta tcgaaaaatt taaagcaaac accaaagcca tttcacgact
     1741 tgcagaacat gttgatacgt cgattgtgct accaccaagc gttgtgagcg cgattcaaaa
     1801 gtcgcaagag tcagcgggag atacatcgaa attcttaaat gaatctaccg acacaatgaa
     1861 tcaagcgtat gatgaaatta aaaaaatcga cgaaaaggca aaacaagttt ctgatgcttt
     1921 agcaaaactt gaaaccgata aaatgctcgt tatcatgatt gataacggac gtctaaaaag
     1981 tgccacctac gaaagcgaaa aagaccaaca gcaatatgag caaattaagc gcaatattga
     2041 agtcttaaga aaacaacgtg aagaacttgt gcgccttaat attgccgcaa agactcccac
     2101 cgaagctgct gcaaacatgc gtaagattgc ggacttagat tttaaaattc aacaagcgca
     2161 agactctact
//
LOCUS       DAOMXO010000417         2169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29538_length_2169_cov_5.049669, whole genome shotgun sequence.
ACCESSION   DAOMXO010000417 DAOMXO010000000
VERSION     DAOMXO010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29538_length_2169_cov_5.049669"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..468
                     /locus_tag="PLH57_08610"
     CDS             94..468
                     /locus_tag="PLH57_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRK02712.1"
                     /translation="MSDLNRIFLMGRVGNELELKTSQNGKPYLRISLATHGNGTSGES
                     TQWHRIVVFGAQAENCARYLQKGSLIFVEGSSETSTYTDADGKKSTSTSVIAYRIQFM
                     SSYSKKSNEDQNDVPAAESASA"
     gene            481..1689
                     /locus_tag="PLH57_08615"
     CDS             481..1689
                     /locus_tag="PLH57_08615"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HRK02713.1"
                     /translation="MLFYEKDVTFPNELSTALEVALAENGQTFSAREINRLGDTLSRL
                     WPELAEKRGIAEQNHYSHRKEFAATYAAYYLPANLLKWACVLDEVQFVAPLANESTKI
                     CDFGCGPGTVYWGAAWWHWKMNRKLEFVGIDQSHQFSGLAPNLAQSLERSLGAQAGTL
                     RARWIDRKLTLESILETVKTERPTMVTFSNALAEIAPKIEDREKIVLALLKTLSVLTD
                     QDSLERKLILLEPASISASRELLELRARLIAHEGVHVELPCLNNRPCGALAREGDWCH
                     EEIAVRFPEWMNQIGKQAGLKKENLLFSYLVLGQKAQTGRLARLPRVVSHRFVEKGLT
                     KCWICTETGKRQIRLLNSRKNEKNESFEGIRRGDLIEELTLSEKSDVQSLLLFRDLDE
                     VADHNAIVPS"
     gene            complement(1630..2043)
                     /locus_tag="PLH57_08620"
     CDS             complement(1630..2043)
                     /locus_tag="PLH57_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02714.1"
                     /translation="MKRVRFTTASLVAGILLILFASTNARAEDFYVTSVVRDFPLSGN
                     EKSPVDYYINAGSVNGLRKGISIEAIRKMPIYDNMNSKLMGDTSVKIARMKVIHVDKT
                     YSVARLVKMYDKTETPLAGHYSVMIGDLIEVSEKQ"
BASE COUNT          607 a          507 c          521 g          534 t
ORIGIN      
        1 aatttttcga gcatcgcaaa ctaagatggc gcgggttttg ctttaggtca actcgcaact
       61 gtaacacttt caatgaaacc aggagtaaaa tctatgtcag acctgaatcg tattttttta
      121 atgggccgcg tcggcaacga actagaacta aaaacttcgc agaacggcaa accctacttg
      181 cgcatttcac ttgcgaccca cggcaatggc accagtggcg aaagcaccca gtggcaccgc
      241 atagtggtat tcggcgcaca ggctgaaaat tgtgcgcgct atttgcaaaa aggttctttg
      301 atatttgtgg aaggtagtag cgaaacaagc acctacaccg acgccgatgg taaaaaatct
      361 acttcaacca gtgtaatcgc ataccgaata cagtttatgt cgtcgtactc aaaaaagagt
      421 aatgaagacc aaaacgacgt tcctgctgcg gaaagtgcct ccgcatgata aatcggtggc
      481 atgctgttct acgaaaaaga tgtaaccttt ccgaatgaat tatccaccgc tctcgaagtg
      541 gcgctcgccg aaaacggtca gactttttcg gcgcgcgaaa taaaccgatt gggcgacaca
      601 ctcagcaggt tgtggcctga gctcgctgaa aaacgtggca tcgcggaaca gaatcactac
      661 tcacatcgta aagagttcgc cgcgacttac gcagcgtatt acttgccggc caatcttttg
      721 aagtgggctt gcgttttaga cgaagtgcaa ttcgttgcac cactcgcgaa cgaatctact
      781 aagatctgcg actttggttg tggccctggt acggtttatt ggggagcggc gtggtggcac
      841 tggaagatga atcgaaagtt agaattcgtt ggaatcgatc agtcgcacca gttttccgga
      901 ttagccccaa atctggcgca atctctggaa cgcagccttg gagcacaggc cggtacactt
      961 cgggcgcgtt ggatcgatcg caagctaaca cttgagtcta ttctagaaac tgtaaaaacc
     1021 gaacggccaa cgatggtgac tttttcgaat gctctcgcgg aaatcgcacc gaagatcgaa
     1081 gaccgggaaa aaatagtgct cgcactactg aaaacgctga gcgtgttgac agaccaagac
     1141 agcctagagc gaaaactaat tcttttggaa ccagcttcaa tttccgcatc gcgggagctt
     1201 ttggagttgc gagcgcggtt gatagcgcac gagggtgtgc atgttgagct gccttgttta
     1261 aacaaccgac cgtgtggagc tctggcacga gaaggcgact ggtgccacga agaaattgca
     1321 gttcgatttc cggaatggat gaaccaaatc ggtaaacaag ctggattaaa aaaagaaaat
     1381 ctcctttttt cctaccttgt actcggtcaa aaagctcaaa ctggacgctt ggctcggctc
     1441 ccgcgagtag taagtcacag gtttgttgaa aaaggcctga ccaagtgctg gatttgtacc
     1501 gaaaccggta aaaggcaaat tcgactattg aactctcgaa aaaacgaaaa aaatgaatcg
     1561 tttgaaggca ttcggcgcgg cgacttgatt gaagagttaa cgctatcgga aaaatcggat
     1621 gtgcaatcgt tactgctttt cagagacctc gatgaggtcg ccgatcataa cgctatagtg
     1681 cccagctagt ggggtttcag ttttatcgta catttttact agacgggcca cgctataggt
     1741 tttgtcgaca tgaatgactt tcatgcgggc aatttttaca gacgtgtcgc ccatgagttt
     1801 gctgttcata ttgtcataaa ttggcatttt ccgaatggcc tcaattgaaa tgcccttacg
     1861 aagaccgttc acgctacccg cgttaatgta gtaatcgact gggctttttt cattgccaga
     1921 taatggaaaa tcccgaacga ctgatgtcac ataaaaatct tccgcacgcg catttgtcga
     1981 tgcaaataga atcaacaaga ttcctgcaac aagactggct gtagtaaatc gtacacgttt
     2041 catgacaaca gttcccctac ccacaaacct atcgtcaggg ttttggcggg acttgagcac
     2101 atcaactgca cctgcagtac ctgcccatca tgagttctta atctttcgca gcaaacaacc
     2161 gaacctatt
//
LOCUS       DAOMXO010000418         2169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29541_length_2169_cov_4.470199, whole genome shotgun sequence.
ACCESSION   DAOMXO010000418 DAOMXO010000000
VERSION     DAOMXO010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29541_length_2169_cov_4.470199"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PLH57_08625"
     CDS             <1..1027
                     /locus_tag="PLH57_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRK02715.1"
                     /translation="TDDHIQEKHEKFKIPIEVNHLVLKWVHFFTVTDRDRFQRFLDNG
                     AVYQNLVKKVLADYGIPTELYYLAMIESGYYLGARSRARAVGPWQFMLGTSRMYGLKT
                     NAFVDERQDIVRSTEAAARYLKALQSKFDSWYLAMAGYNAGEGRIAGAIRRGRSRDFW
                     VLVEKKVLPPETRDYVPKFLAAVIVGRNLKKYGFESSESIEKFPTVRTVSVPGSVRLD
                     DIAKSIDMRASDLKRLNRHILRDITPVGMRRYGVWVPVERVAQLEMSKSNLARHRIAP
                     VREKKRYVASVRKRGRVRYHHVRRGEALASIANQYNVSTEKLKRLNRLKSNLIYAGQR
                     LVLPKNL"
     gene            complement(1149..1226)
                     /locus_tag="PLH57_08630"
     tRNA            complement(1149..1226)
                     /locus_tag="PLH57_08630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1189..1191),aa:Arg,seq:acg)
     regulatory      1306..1421
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1409..>2169
                     /locus_tag="PLH57_08635"
     CDS             1409..>2169
                     /locus_tag="PLH57_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK02716.1"
                     /translation="MNRFFLIFYILGLLGCSVVLAEESIFRVRLVEDLGTLDWGYGEV
                     NAQVVDQLMEGLTFVDRRGNATPAVARKWVSTGNITQFFLRSDAKWSDGRRVCAEDFE
                     IAWQRIREAQIVSPYSHLFKDIQAVRAKSCEKLEVETKTPLSYLPELLSHWVFYPVQK
                     NSYQKQRDKFFEPENLISNGPYKIKKWHKDSRYVLEKNEYYFAEKARPLLLEFLVVSD
                     DQTALTLYKAGKIDWLKDPPILSRDQIVRSEGFRV"
BASE COUNT          577 a          475 c          559 g          558 t
ORIGIN      
        1 caccgatgat catattcaag aaaaacacga aaaatttaaa ataccgatcg aggtgaatca
       61 tctcgttttg aagtgggtac actttttcac ggttaccgac agagatcgat ttcaaagatt
      121 tttagataac ggtgcggtct accagaactt ggttaaaaaa gtattagccg actatggcat
      181 tcccaccgag ctttattatt tggcaatgat cgagagtggc tattatctcg gagcgagatc
      241 gcgcgcccgt gcagttggcc cgtggcagtt tatgcttgga acgtctcgaa tgtacggttt
      301 gaagaccaac gcgtttgtcg atgagcgtca ggacatcgtg cgttcaacag aagccgccgc
      361 gagatattta aaggcgctcc agtctaaatt cgattcatgg tatttagcga tggccggata
      421 caacgctggc gaaggacgaa ttgccggcgc catacgccgg ggtcgcagtc gagatttttg
      481 ggtacttgta gaaaagaaag tattgccgcc agagacccgc gactatgttc cgaaattttt
      541 agctgccgtc attgttggtc gaaatttaaa aaaatatggg ttcgaatcaa gcgagagcat
      601 cgagaaattc ccgactgtgc gaacagtgtc ggtcccaggt tctgtccgac tcgacgatat
      661 tgccaaatct attgatatgc gggcatccga cctcaagcgc ctgaatcgcc acattttgcg
      721 agatattacc ccggtcggaa tgcgacgcta cggtgtttgg gtaccagtcg aaagggttgc
      781 gcaattggaa atgtccaagt caaatcttgc gcgtcaccga attgcgccgg ttcgtgaaaa
      841 aaaacgatac gtggcgtccg ttcgtaaacg cggtcgcgtt cgatatcacc atgttaggcg
      901 tggagaagct cttgcttcga tcgcgaatca atacaatgtt tccacggaaa aattgaaacg
      961 tctaaatcgt ttgaaatcaa atcttatcta cgccggccaa cgattggtgc ttccaaaaaa
     1021 tttgtagttt agagaaaaaa tggcgcactc ggcagggttg gctcgctttg ctcgcccaga
     1081 accgtagaac ccaagtgcag caaaaataaa aaagccgtcg aggcgttgcc ccaacggctt
     1141 ttttattatg gcgcacccgg cagggttcga acctacgacc ttcagatccg tagtctgacg
     1201 ctctaatcca gctgagctac gggtgcaccg ctacgttaaa taggtgacag tcttataggg
     1261 ttttttggct tggagggcaa gtgtttttca ggtaatcccg ggggaaaaca ccagcggggg
     1321 agctgctaat gtctgcggct gagaggctcg attttgattc gggcgacccg aaaacttgat
     1381 ccggataatg ccggcgtaag gacatctaat gaaccgtttt ttcttaattt tttatattct
     1441 tgggctgctt ggctgttcgg tcgtcttagc ggaagaatcg atattccgtg tgcggctggt
     1501 tgaagacctg ggcacgttgg actggggcta tggcgaagta aacgcccagg tcgtagatca
     1561 gctaatggaa ggtctaacct ttgttgatcg aaggggcaat gcgacgccag cggtcgcgcg
     1621 aaagtgggta tcgacaggga atatcacgca gtttttttta cgctccgacg ccaagtggtc
     1681 agatggccgc cgtgtttgtg ctgaggattt cgagattgcc tggcaacgaa ttcgtgaagc
     1741 gcagatagta tcgccctatt ctcacctgtt taaagatatt caggcggtgc gggctaaatc
     1801 ctgcgagaaa ctagaagtcg aaaccaaaac tcccctttcc tacctaccgg aactactaag
     1861 tcattgggtg ttttatccgg ttcaaaaaaa tagctatcag aaacagcgcg ataaattctt
     1921 tgagcccgaa aatttaattt ctaatggacc gtataaaata aaaaaatggc acaaagattc
     1981 tcgttatgta cttgagaaaa atgaatacta tttcgccgaa aaagcgcgcc cgcttctgtt
     2041 ggaattttta gttgtttcag acgatcaaac cgccctcaca ctgtataaag caggaaaaat
     2101 tgattggtta aaggacccac cgatattgag ccgggaccag atagtgcggt cagagggttt
     2161 tcgagtgtt
//
LOCUS       DAOMXO010000419         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29623_length_2165_cov_3.756872, whole genome shotgun sequence.
ACCESSION   DAOMXO010000419 DAOMXO010000000
VERSION     DAOMXO010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29623_length_2165_cov_3.756872"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLH57_08640"
     CDS             complement(<1..487)
                     /locus_tag="PLH57_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HRK02717.1"
                     /translation="MAKISKKKSAKPVARNKGINFVEQYCDAFVQFGATRSNTASEQM
                     AEVVAVAPGVGNGEKNRSSFTLTKDCKDYTTKCAELIQSQELSTAAGSTCLVRLGGLR
                     KAKNLLFVGLGKEKKNYDFASKSERLRQIGGAIARKLVAEKQSSANVVLDSFLNSASN
                     TN"
     gene            581..1975
                     /locus_tag="PLH57_08645"
     CDS             581..1975
                     /locus_tag="PLH57_08645"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRK02718.1"
                     /translation="MLRKYYAVFGPALRLMDASVLLFCWLAAYYLRKHYPLAIMTNAI
                     PPFDEYAAYGVVIVFLWGSVFSIFNLYSSQRMVRRTAEAYRVLRAHTLACLIFIALTY
                     LVSTYKLSRGVFVYFYFGSAVLLVFGRIQLRNTLRRFRAKGFNQNAVLLVGSGVPAGH
                     TYSKLSRHPELGLRFVGFAAPKGTEPPVKGLAWLGTYEEIAKIAKEHQVDKVVVALSR
                     NEMPQLEAILGSVKDEILDVVLVPDIYDYIAIGCEVEDFDGLPMVSLNETPMMGFNML
                     LKRVSDIFLAMVALIIAGPVMVLLAALIKLTSKGPVFYRQERMSLNGKRFGMIKFRSM
                     TVDQIGDVELLTKKDDPRVTAIGRIMRKTSLDELPQFFNVLLGDMSIVGPRPERTWVV
                     DKVRSQVPRYMLKHKVKAGITGWAQVNGWRGDTSLEKRIEHDLYYIRHWSLLFDLKIL
                     FLTVFRGLIGKNAY"
BASE COUNT          508 a          484 c          540 g          633 t
ORIGIN      
        1 cgttggtgtt ggacgctgaa ttcaaaaacg aatcgagtac tacgttcgca gaagactgtt
       61 tttcggccac tagttttcta gctatggcgc ctccgatttg acgtagacgt tctgatttag
      121 atgcaaagtc gtagtttttc ttttcttttc cgagtcccac gaaaagtaag tttttagctt
      181 ttcgaagccc gcctaatcgc accaagcagg tagagccagc agcagttgaa agttcttgag
      241 attgaatcaa ttcggcgcat ttagtcgtat agtctttgca gtccttagtg agcgtaaagg
      301 aactgcggtt tttttcgcca tttccaacgc cgggggctac ggccaccact tctgccattt
      361 gctcggaagc ggtattggat ctagtcgccc cgaattggac gaatgcgtcg cagtactgtt
      421 cgacgaagtt tatacccttg tttcgggcaa ctggtttcgc ggacttcttt ttgctgattt
      481 tagccataaa aactccctta gagttcgtaa gaaaatgaag ctccgatcta tcaaaaatac
      541 ctgcgttgca aaagctctaa agcgccggta ttgatgagat atgcttcgaa aatactacgc
      601 ggtcttcggc ccagcgcttc gcttgatgga cgcgtcggtt ttgctcttct gttggttggc
      661 ggcctactat cttcggaaac actatccgct cgcgataatg accaacgcta ttccgccgtt
      721 tgacgagtac gcggcctatg gcgtggtaat tgtttttctg tggggctcgg tcttttcaat
      781 atttaattta tactcaagcc aaagaatggt tcgccgaacc gctgaggcct accgagtcct
      841 tcgagcccat actctggcct gtttaatatt cattgcgctg acatatttgg tgtcgactta
      901 taagctctcc cgcggggtat ttgtttattt ctactttggc tcggcagtct tgttggtgtt
      961 tggtcgaatt caactgcgca atacgctgcg tcgattccgg gcgaaaggat ttaatcagaa
     1021 cgcggtgcta ctcgttggat ctggggttcc cgccggccac acctactcca agttgtctcg
     1081 gcaccccgaa ttaggtttgc gattcgtggg tttcgcggcc cctaagggca ccgaaccccc
     1141 cgtaaaaggc ttagcttggc tgggtaccta cgaagagatc gctaaaatcg cgaaagagca
     1201 ccaggtagat aaagtggttg tcgcactttc gcgaaacgaa atgccgcagt tggaagcgat
     1261 tttgggttcg gttaaagacg aaatacttga tgtcgtatta gtgccagaca tttacgacta
     1321 tattgcgatt gggtgcgaag tagaagattt cgacggtttg ccgatggtca gtttaaatga
     1381 aaccccaatg atgggtttta acatgttgct caagcgagtg tccgacatat ttttggccat
     1441 ggtcgctttg ataattgcag ggccagtcat ggtattgttg gcggccttga ttaaactgac
     1501 ttcgaaaggc ccagtgtttt accgtcagga acgcatgagc ctcaacggca aacgatttgg
     1561 aatgattaag tttcggtcga tgaccgttga tcaaattgga gatgtagaac tacttactaa
     1621 gaaagatgat cctcgtgtca cggccattgg ccggataatg cgcaaaacaa gcctcgatga
     1681 gttgcctcaa ttttttaatg tgctactggg cgacatgagc attgtcgggc cgcgcccgga
     1741 acgaacctgg gtggttgata aagtgcgctc tcaagtgccg cggtacatgc tgaagcacaa
     1801 agtcaaagcg ggtatcactg gttgggcgca ggtgaatggc tggcgtggcg atacttcact
     1861 cgaaaaacga atcgaacatg atttgtacta cattcgccac tggtcgttat tgtttgacct
     1921 caagatcttg tttctcacgg tattcagagg tctaataggt aagaacgcct actaactata
     1981 gggcgtagaa atcaaaacat gagagcttac cgctatatac ttaccgaagt gttagttcca
     2041 tttttcggag cgttcttttt tttcatgttc gtatttctca tgttccaagt ggtgcggctt
     2101 actgattact tcattaatca tggagtgggc ctgacgcttc taaccaagat gaccatttat
     2161 atttc
//
LOCUS       DAOMXO010000420         2161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29692_length_2161_cov_5.445869, whole genome shotgun sequence.
ACCESSION   DAOMXO010000420 DAOMXO010000000
VERSION     DAOMXO010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29692_length_2161_cov_5.445869"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..>2161)
                     /locus_tag="PLH57_08650"
     CDS             complement(213..>2161)
                     /locus_tag="PLH57_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK02719.1"
                     /translation="DVKKKYKMGEQTLEILKGVSLTIEDGEYVAIMGPSGSGKSTLMN
                     ILGLLDVPSEGSYKLNGVEISQMSEDELSEVRRGEIGFIFQQFNLLPRMTAVENVALP
                     LLYSKRFLGSERAQRLLASVGLQERGHHRPSELSGGQQQRVAISRSLINSPKMVLADE
                     PTGNLDSQSQAEIMNILKKLNDSGITIVIVTHEEEVAKTAHRLIRIRDGVIQSDERRP
                     LTPSKPTNLESLHEKPVVVEKNSFFQDILDYFRQGFKTLAANKVRTGLSMLGILIGVA
                     AVVAMLALGKGAQKAIEEQLSSLGSNLLTVRPGSVRIGGVTQESGAANRLLLEDIDII
                     STRDKNVARISGIVNGRVQVRSSTNNWNSSVSGVSPHWASMHTSVPPIGRFFTEEENK
                     KRALVAVIGETVRRELFGDKSAIGEMIKINRINFQVIGILPEKGANSFRDQDDTVVVP
                     VETAMKRLLGRTNLEMIEIEINSAENIEQSIDRITQILTDQHKIPLSQQSEAFQIRNM
                     ADIQKAMQQSSETMSMLLAAIATISLLVGGIGIMNIMLVSVTERTREIGLRKALGAKR
                     KDILSQFLSESLVVSVVGGMFGILLGWSLTLILSSVIGWTTYVSLSSVLVSFFFSAMI
                     GIVFGVYPARKAALLNPIDALRRD"
BASE COUNT          592 a          553 c          422 g          594 t
ORIGIN      
        1 tcaccagccg aaaagaaata aacgaatttt tctggaactt taagtgcggt ctgagtgctc
       61 tcagtcgtct gcatggacta accccttttt tgagaatgaa cgtaccgcca gagttagcaa
      121 ttggtcgcaa aatagtctat cggaactccg taattaacag ggtgcagtca aaactttcgg
      181 gcccaaaatg caattactct attttcagaa tcctaatcgc ggcggagagc atcgatggga
      241 tttaataaag cggcttttcg tgccggataa acaccgaaca caattccaat catcgccgag
      301 aaaaagaacg aaactaaaac cgaggaaagc gatacatagg ttgtccaccc aataacagat
      361 gacaatatca gtgtaagact ccagcccaac aaaataccaa acataccgcc gactacgcta
      421 accaccaaag attcggaaag gaactgagac aagatgtcct ttctttttgc gcctaaggct
      481 ttacgcagac caatttcccg cgttcgctcg gtaacggaga ccaacatgat attcatgatt
      541 ccaatacctc caaccagtaa cgaaatcgtg gcaatcgctg ccaacagcat agacatagtt
      601 tcgctacttt gttgcatcgc cttttgaata tcggccatgt ttctgatctg gaaggcttca
      661 ctttgttgag agagcggaat tttatgctga tccgtaagta tttgggtaat acgatcaatc
      721 gactgctcga tattttccgc agagttgatt tcgatttcaa tcatttccaa attggttcgt
      781 cccaataatc gtttcatggc cgtttcgacg ggtacaacca cagtatcgtc ctggtcccga
      841 aaactattcg cccctttttc aggaagaatg cctatcacct gaaagttgat acgattaatt
      901 ttaatcattt caccaatcgc ggacttatct ccaaacagtt cacgccgaac cgtttctcca
      961 atgaccgcaa ctaaggctct ttttttattt tcctcttcag taaagaaccg tccgatgggt
     1021 ggcaccgatg tatgcatcga ggcccagtga ggcgaaaccc cgctcacact cgagttccag
     1081 ttgttggtgg aactacgcac ttgaacacga ccgttcacaa tgccggaaat acgcgctacg
     1141 tttttgtcac gcgtactgat gatatcgata tcttctagca gtaaccgatt cgctgcgcct
     1201 gattcttggg tgacaccacc gatacggacc gagccgggac gaaccgtcaa caagttagat
     1261 cctagtgatg aaagctgctc ttcaatagct ttttgtgcgc ccttgcccaa tgccaacatc
     1321 gcgacaactg ccgccacacc gatcaaaatt cctagcatgg acaatcccgt gcgcactttg
     1381 ttagccgcta acgtcttaaa cccctgtctg aaatagtcta aaatatcttg aaaaaaacta
     1441 tttttttcaa ctactaccgg tttttcatgt agggattcaa gattggtcgg cttcgagggc
     1501 gtaagtggcc gacgctcgtc cgactgaata accccgtcgc gaatacgaat caatctatgc
     1561 gctgttttcg ccacctcttc ctcatgagtc acgataacaa ttgtgattcc ggaatcattc
     1621 agttttttga gaatattcat aatttcagct tggctttgcg aatctaaatt tcctgttggt
     1681 tcatctgcta aaaccatttt tgggctgtta ataagagacc tagaaatcgc aactcgttgc
     1741 tgctgcccac ccgacaattc actgggccga tggtgtccac gttcttgtaa gccaacggac
     1801 gccagcaatc tctgcgcccg ttcgcttcca agaaagcgtt ttgaatataa taatggcaac
     1861 gccacatttt cgactgcggt catgcgcggt aacaaattga attgttgaaa tataaaaccg
     1921 atttcacctc gacgaacttc agacagttca tcttcactca tctgcgaaat ctcaacgcca
     1981 tttagcttat atgacccttc gctaggcaca tccagcaaac ccaagatgtt catcaacgtc
     2041 gactttccag atccagatgg gcccatgatc gcaacgtatt caccgtcctc aatcgtcaag
     2101 ctcactccct ttagaatttc taaagtttgt tcgcccattt tatatttctt tttaacatct
     2161 t
//
LOCUS       DAOMXO010000421         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29733_length_2159_cov_4.222909, whole genome shotgun sequence.
ACCESSION   DAOMXO010000421 DAOMXO010000000
VERSION     DAOMXO010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29733_length_2159_cov_4.222909"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PLH57_08655"
     CDS             <1..1157
                     /locus_tag="PLH57_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK02720.1"
                     /translation="LITAISVGGVAVGVYTLIVTLSVMAGFESELTKRLFASDTHIRI
                     EPESGSTLVRDPNVIGKIAQLDTKITGVYPVFQTEIILRAGKKVSGSVLKGIGPDQLR
                     WLKSMVSEWAATELIDPSVDGRLFIGSELAFDIGVIPGDVITTVSPVPTDGPLGAVPR
                     VKKFVVEGVYKSGIPEQELHVVFAEMKHVESFLRKDDVWSQIEVRVDSLKNAPNVAAK
                     LKAENLVAHSLVRPWQEINAHLFSSLKLERTAMFCILIFIVIVASFNITSTLTMMVVE
                     KRRSLSILRAMGATPRHIRSIFQSEGLAIGLVGIAIGGLLAFGTCWFLRKYPLLELPE
                     YFFDRTVPVVLQPLGIMLVCFCALIIVMVAARVPARRAGLLTPIEGIRNQ"
     gene            1246..1893
                     /locus_tag="PLH57_08660"
     CDS             1246..1893
                     /locus_tag="PLH57_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02721.1"
                     /translation="MAQAEGDESIVVDIDSEEQAWQGIQTRAGNYQQLDGAVVVPAED
                     VTDVLGNSIPFIDAFLGPLADELESVDIDRKDNGIEMKLHLLRAIKFPVASKNKKKSK
                     KGEIVRVEVDREVAMTFRNEAFRIRFHHISGIGVVIRMPPILRIPGFSMQRLDLSAIN
                     IDFKKQKLSFMVGAANRMVRLTASVDLKEGRFEGINIWETLALNGPALIALLGLL"
     gene            complement(1868..>2159)
                     /locus_tag="PLH57_08665"
     CDS             complement(1868..>2159)
                     /locus_tag="PLH57_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02722.1"
                     /translation="SKSPSFEASRSLLKIVEMRFLNRFEGRSQRPEILQKQKPICRLQ
                     FELANRKETLDVYSDASGGLWVRNTNLPNVQFEVKKELVTKVDSLKAVPATQ"
BASE COUNT          529 a          471 c          581 g          578 t
ORIGIN      
        1 cactcattac tgcaatcagc gtaggtggag tggccgtcgg ggtttatacg ctgatcgtta
       61 cgctctctgt aatggcaggc ttcgagtctg agctgacgaa gagactgttt gcttcggaca
      121 cccacattcg cattgaaccc gaaagtggca gtacacttgt tcgagacccg aacgtcatcg
      181 gtaagatcgc gcagctcgac actaaaataa ctggcgttta tccggtgttt cagaccgaaa
      241 tcattttacg ggcagggaaa aaagtttccg gaagcgtatt gaagggaatc ggtcccgatc
      301 agttgcgctg gttaaaatca atggtttcgg agtgggccgc cacggaactc atagatccct
      361 cagtcgatgg gcgcttgttc attggttcgg agctggcgtt cgacattgga gtgattcctg
      421 gagatgtaat cacgacggta tctcccgtac caactgacgg gccacttggg gccgtgccgc
      481 gagtaaagaa attcgtcgtc gaaggcgttt ataagtctgg gattccagag caagaactcc
      541 atgtcgtgtt tgctgaaatg aaacacgtgg agtcgttttt acgaaaagat gacgtatggt
      601 cacagataga ggtgcgagtc gattcactta aaaacgcacc aaacgtggct gccaaactga
      661 aggccgaaaa cctagtggcg cattcgctag tgcgaccgtg gcaggagatc aacgcccacc
      721 tttttagttc actcaaactc gaacgaacgg caatgttctg cattttaata tttatcgtga
      781 ttgtcgccag cttcaatatt acgagcacgt tgacgatgat ggtggtcgaa aaacgtcggt
      841 ctctttcgat tctacgtgcg atgggagcaa caccgcgaca tattcgctcg atttttcaga
      901 gcgaggggct ggccattggt ctagtcggaa ttgcgattgg cgggctgctg gcatttggaa
      961 catgttggtt tttaagaaaa tacccgcttt tagagctgcc ggagtacttc ttcgaccgaa
     1021 cggttccagt ggttttacaa ccccttggaa ttatgctcgt atgtttttgt gcacttatca
     1081 ttgtgatggt ggcagctcga gttcccgctc ggcgagcggg cctgttgaca cccatcgagg
     1141 gaatacgaaa tcagtaggtg tgatgcaatg ggtttttcta agatgagatt tccgaagatt
     1201 gtcgtgttat gcggattgtt ttgtttggca acggcgacat cgagtttggc acaagctgaa
     1261 ggcgacgaat ccattgttgt agatatcgat tcggaagagc aagcctggca gggcattcaa
     1321 acgcgagctg gcaattatca gcagctcgac ggtgcagttg tggtgcccgc cgaggacgtc
     1381 acggacgtgc tcggaaattc gattccgttt atcgacgcgt ttttgggacc actcgctgac
     1441 gaactcgaat cagtcgatat cgatcgtaaa gataacggca tagaaatgaa attgcatttg
     1501 ctgagagcga ttaaatttcc ggttgcctcc aagaacaaga aaaaatcgaa aaagggcgaa
     1561 attgttaggg ttgaagtcga ccgtgaagtg gccatgacct ttaggaatga agcttttcga
     1621 attcgcttcc atcatatttc gggtattgga gtagtgattc gcatgcctcc aatattacga
     1681 atccccgggt tctcgatgca gcgcctggat ctgagcgcga tcaacattga ttttaaaaaa
     1741 caaaaactaa gttttatggt tggggcggcc aatcgcatgg tgcgccttac cgcctcggtg
     1801 gacctgaaag agggaaggtt cgaaggcatc aatatttggg agaccctcgc tcttaacggg
     1861 cctgcactta ttgcgttgct gggactgctt taagggaatc tactttggtt actaattcct
     1921 ttttcacttc aaactgaacg tttggaagat tcgtgttgcg aacccataaa ccgccgctag
     1981 cgtcgctata gacgtcgagg gtttcctttc gattagctaa ttcgaattga agtctgcaga
     2041 ttggtttttg cttttgaaga atctcgggcc gttgacttct accttcgaaa cggttcaaga
     2101 agcgcatctc tacgattttt aaaagtgacc gagaggcttc gaaagagggg ctcttgctc
//
LOCUS       DAOMXO010000422         2156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29789_length_2156_cov_5.031414, whole genome shotgun sequence.
ACCESSION   DAOMXO010000422 DAOMXO010000000
VERSION     DAOMXO010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29789_length_2156_cov_5.031414"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLH57_08670"
     CDS             complement(<1..306)
                     /locus_tag="PLH57_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRK02723.1"
                     /translation="MSEKNLEIFSPNRISFLTTDSREVQPGCGYFAVKGTNFDGRLFI
                     KDAVELGASVVVTDSPSVPAEFTGTKTQIIVVKDIRKFLAEASCHFFEHPTRKLKLLG
                     "
     gene            complement(269..>2156)
                     /locus_tag="PLH57_08675"
     CDS             complement(269..>2156)
                     /locus_tag="PLH57_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRK02724.1"
                     /translation="LFIRPELMRKELNASERARLSNIISKGIHWPKQRVIEKIRSDRG
                     FVWVKRQLSQTEESFLRSHKILEFGDSVGLVEETRRFYPNKELAAHVLGSVGIDGQGL
                     EGLELLYDAILSGEQSKIDSSRDARGRRIFRGENGLFAFKDGQSLVLSIDRTIQFDAE
                     KALDQAVREFGAKAGTAIVAEVETGEILALANAPTFNPNVANQYSADSRRNRAITDAY
                     EPGSTFKPLIAGLALENGKTTRSKIYCEMGSFKVADRVISEAETHEQWGWLTLGEVIQ
                     HSSNIGAAKIALELGVDSITGFFDKLGVGKKTGLDLPGEAPGRYSSSEARSSVRLANV
                     GFGQGFTTTSLQMLSFYLSIANNGQWIQPRIVKAILNEDPESIVRGTLRWKLDHRFEN
                     SKSKKIFEPKTAKDITRMLELVVNENGTGKLAQLEDWPVAGKTGTAQKIDQETYRYSR
                     SKYISSFVGFAPAKNPKLVTLVMLDEPTKKYYASETAAPVFREIMQAALIRTQVPPVD
                     SKVRLMKLANQSSIDAIEESTEATAPRGGSESLASGVAKSPDVKLKRNDKNEIVLPDL
                     RGLSIREAVRAIGTEPVKLEIHGSGIVREHFPKPHETLEPGGRLRLVCSSHERKKSRD
                     LFPE"
BASE COUNT          514 a          513 c          527 g          602 t
ORIGIN      
        1 acccagcagt ttaagttttc gtgttgggtg ttcaaaaaaa tggcaagaag cctctgccaa
       61 aaactttcgg atatctttta caacgatgat ttgagttttg gtcccggtaa attcagcggg
      121 gacggacggc gaatctgtaa ccaccaccga agcaccgagt tcgaccgcgt ctttaataaa
      181 cagtcgtcca tcgaagttgg tgcctttaac cgcaaaatag ccgcaaccgg gttggacttc
      241 acgactatcg gtcgtgagaa acgagatcct attcggggaa aagatctcta gatttttttc
      301 gctcatgaga gctgcatact agccggagcc ggccaccggg ttcaagggtt tcgtggggtt
      361 ttgggaagtg ttctcgcact attccggagc catgaatttc cagtttaaca ggttcggtac
      421 cgatcgcgcg gaccgcctcg cgtatagata gtccgcgcaa atctggaaga acgatctcgt
      481 tcttatcatt gcgcttcagt ttgacatcag gcgatttggc aactcccgaa gctaatgatt
      541 cggacccacc acgtggggca gtcgcttcgg ttgattcctc tattgcgtcg attgaacttt
      601 ggtttgctag cttcatgaga cgaaccttac tgtcgacagg cggtacctgg gtgcgaatga
      661 gtgccgcctg cataatttct ctaaaaactg gggcagctgt ctctgaagcg tagtatttct
      721 ttgtaggttc atcgagcatg accaatgtga ctaatttggg gtttttagct ggagcgaagc
      781 caacgaaact cgaaatgtat ttcgaccgcg agtaacgata ggtttcttga tctatttttt
      841 gagcagtacc ggttttgccg gcgacgggcc agtcttcaag ttgggcaagc tttccagtgc
      901 cattttcgtt aactacgagc tcaagcattc gagtaatgtc tttcgcagtt ttgggctcaa
      961 atattttttt tgatttagaa ttttcaaaac gatgatcaag tttccagcga agtgttccgc
     1021 gtacgattga ttcggggtct tcattgagta tagctttaac gattcgaggt tggatccatt
     1081 gcccattatt ggcgatcgac aaataaaacg aaagcatttg cagcgaagtt gtagtgaaac
     1141 cttggccaaa acctacgttg gcaaggcgta cgctcgatcg ggcctctgat gatgaatacc
     1201 ttccgggagc ctctcccggc aggtccaggc cggtcttttt tccgacgccg agtttatcaa
     1261 aaaaaccagt aatggagtcg actcctagct caagcgcaat cttggcggct ccaatattgc
     1321 tggagtgctg aatgacttcg cccagtgtca gccaccccca ctgttcgtga gtttcagctt
     1381 cggagatcac tcgatcagca actttaaatg agcccatttc acagtaaatc ttagaccgag
     1441 tggttttgcc attctccagc gccagaccag cgatcagggg tttgaacgta ctgccgggtt
     1501 cgtaagcatc ggtgattgca cgattgcggc gtgaatcggc tgagtattga tttgcgacat
     1561 tcggattgaa ggttggggca ttagcgagag ctaaaatttc cccggtttct acttctgcga
     1621 caatcgcggt gccagctttt gcaccaaatt cgcgcactgc ctgatcaagc gctttttcag
     1681 cgtcgaattg aatagtgcga tcaattgata acactaagga ttggccgtct ttaaacgcga
     1741 agagtccgtt ttctccgcga aaaatgcgtc taccgcgtgc gtcgcgtgac gaatcgattt
     1801 tcgactgttc gccacttaaa atagcatcgt agagcaactc caagccttct aagccctggc
     1861 catcgattcc aacgctaccc agtacgtgcg cggcgagttc tttattcggg taaaagcgac
     1921 gcgtttcttc cactaagccg accgagtcgc cgaattctaa aattttgtgg gacctcaaaa
     1981 agctttcttc ggtttggctt aattgccttt ttacccaaac aaatccccga tcagatcgaa
     2041 ttttttcgat gactcgctgt tttggccaat gaatgccttt cgaaataata tttgataatc
     2101 gtgcccgttc cgaagcgttc aactccttac gcatgagttc ggggcgtata aaaaga
//
LOCUS       DAOMXO010000423         2148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_29943_length_2148_cov_3.918777, whole genome shotgun sequence.
ACCESSION   DAOMXO010000423 DAOMXO010000000
VERSION     DAOMXO010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29943_length_2148_cov_3.918777"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLH57_08680"
     CDS             <1..795
                     /locus_tag="PLH57_08680"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRK02725.1"
                     /translation="GFFYHPDREKYFDPHKNGFNPQDVWFTAGDGAKLHGWWFAVPNA
                     VGTIVQFHGNAENLTSHFASLAWLTRERYNLFIFDYRGYGQSPGNPDAAGLYLDGMAA
                     LDTAWDLHQRTSRSSNRFVVFAQSLGVIVATRAFSDFQHRDATWMLVLDSGFCSFKDL
                     VQEKMADIWITWPFSPLGRLSVSDRYACRSVLPSLRVPTLVIHDENDPVVDIRNSEMI
                     FDALTQQEKHFWRISEGRHVAVFAIDQPNRRKLFLDLLSSRLARVK"
     gene            811..1278
                     /locus_tag="PLH57_08685"
     CDS             811..1278
                     /locus_tag="PLH57_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02726.1"
                     /translation="MLTATKENRDGKQFVLLGGTIDETDDLAKVIGMPTGPLIVSCRD
                     LTRINSVGVKNWMKYFSQLQQNKLQFEFEELSVALVEQVNIVKNFACGGPVLSIAMPF
                     RCNSCQTGLTGAIKTEVVRSMNFNIAPVQCPKCGGSAEFDDVAEEYFSFLTRS"
     gene            1290..>2148
                     /locus_tag="PLH57_08690"
     CDS             1290..>2148
                     /locus_tag="PLH57_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02727.1"
                     /translation="MSVNKLVLVSSGPEDLDFALKVCQRFRIQLAVPKSWHELLETLS
                     ESYSTYIFWALGDLELHDASHPFFIGAIEEALTGQVDPSRVFALTTKPIHHYQDMFEK
                     FPTIKGRFQHNLIRNYPPGCEILYARLLEYCSAPEPSGLQKLFDPTTKIQRLALKNSS
                     QKNSAVSALEKTLLKTGFDERIASRIAQATDELLMNAIFDASRDSSGVANRQMLDRRV
                     HFAFGPQEPVEMEFGLNQEYFAICVTDHFGSLKKETLFKFLHQNYQDKDYQLRKYGPG
                     AGLGLYGIVA"
BASE COUNT          533 a          556 c          502 g          557 t
ORIGIN      
        1 ggtttttttt accatcctga tcgtgaaaaa tattttgacc cccataaaaa tggttttaac
       61 ccgcaagacg tttggttcac tgctggagac ggcgccaagc tccacgggtg gtggttcgcg
      121 gtgccaaatg ctgtaggcac gatcgtgcag tttcatggca acgccgaaaa tctaacgagc
      181 catttcgcca gcctggcttg gcttactcgg gaacgctaca atctttttat tttcgactac
      241 cgaggttacg ggcaatctcc gggcaacccc gacgcggcgg ggctatactt agacggcatg
      301 gctgcgctcg acaccgcgtg ggatttgcat cagcgcacgt ctaggtcgtc gaaccgtttc
      361 gtggtctttg cccaaagctt gggagtaatc gtcgcaacac gtgccttttc ggactttcaa
      421 caccgcgacg ccacttggat gctagtgttg gactctgggt tttgctcttt taaagacctc
      481 gtgcaggaaa aaatggcaga catctggatc acctggcctt tcagtccgct tggccgccta
      541 agcgtttcgg atcgatacgc ctgtcgttca gtgttacctt cacttcgcgt tccaaccctt
      601 gtcattcacg atgaaaacga cccggtcgtt gatattcgca attctgaaat gattttcgac
      661 gcactcaccc aacaagaaaa acatttctgg cgaattagcg aggggcgcca cgtcgcggta
      721 ttcgcgattg atcaaccgaa tcgaagaaaa ctctttttag acctcttgtc gtctcgcctc
      781 gctcgggtaa aatgaatcaa agaggaatac atgctcaccg caaccaaaga aaatcgcgat
      841 ggtaaacaat ttgttttgtt aggtggcaca atcgacgaga ccgacgattt ggcaaaagtg
      901 attggaatgc cgaccggccc cttgattgtt tcttgccgag atttaacgcg aataaattca
      961 gtcggcgtaa aaaattggat gaagtacttt tcacaactcc aacaaaataa attgcagttt
     1021 gaattcgaag aactttcggt agcgctggtc gagcaagtta atattgtcaa aaacttcgct
     1081 tgcggcggcc cagtgctttc gatcgccatg ccgtttcggt gtaattcgtg tcaaacggga
     1141 ttaaccgggg ccatcaagac agaagtcgta cgatcgatga atttcaatat tgcaccggtt
     1201 cagtgtccaa aatgtggcgg atcggcggaa ttcgacgacg tggccgaaga atatttttcg
     1261 tttctcacgc gcagctagag ggtcgcacca tgagcgtcaa taagctagta ctggtgtctt
     1321 cgggccctga ggatctcgac ttcgcactaa aagtttgtca gcgttttcga attcagttgg
     1381 ccgtgcctaa atcctggcat gaactactag agactttgag tgaatcctac tccacctata
     1441 ttttctgggc cctcggtgat ttggaacttc acgacgcctc tcacccgttt ttcatcggag
     1501 ccattgaaga ggcactcacc ggccaagtcg acccttctcg agttttcgct cttacgacta
     1561 aacccataca ccactaccaa gatatgtttg aaaagttccc cacgatcaag ggccgctttc
     1621 agcacaattt gatccgcaat tatccgccgg gctgtgaaat tctttacgct aggctacttg
     1681 aatactgttc tgctcccgag ccatcagggc ttcaaaagct ttttgatcct acgactaaga
     1741 ttcaacgact cgcgctcaag aattcttccc aaaaaaactc cgcggtttcc gccctcgaaa
     1801 aaactctgct taaaaccgga ttcgatgaac gaattgcgtc ccgcattgcg caggcgactg
     1861 acgaactact gatgaacgca atattcgacg catcacgcga ctcctcaggg gtggccaacc
     1921 gtcaaatgct cgatcgccga gtgcatttcg cctttggccc tcaagaaccg gtggaaatgg
     1981 agttcggcct caatcaagaa tattttgcga tctgcgtcac agaccatttc ggctccttaa
     2041 aaaaggaaac tctatttaaa tttcttcatc aaaattacca agataaggac taccagctcc
     2101 gtaaatacgg tcctggagcc ggtctcgggc tctacggcat tgtggcgg
//
LOCUS       DAOMXO010000424         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30051_length_2142_cov_6.151414, whole genome shotgun sequence.
ACCESSION   DAOMXO010000424 DAOMXO010000000
VERSION     DAOMXO010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30051_length_2142_cov_6.151414"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLH57_08695"
     CDS             <1..561
                     /locus_tag="PLH57_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02728.1"
                     /translation="LDSLNKKVGELDFAQGDYEITLTYVSGYDELFADLVAVILTEDP
                     KAIEKSVDFAGMRSYVSHSRFDQWAKHDDAVSMRLSTKQERARLSGRLNARRFSSRPG
                     DGIAEMGAIVSPHELFAGTRAFLGKYYLSHHNYRNNPKVILEKVFDAIVRQIKVLQSN
                     PKKFAEKTLKEINQDLNETIEELLPR"
     gene            complement(633..1925)
                     /locus_tag="PLH57_08700"
     CDS             complement(633..1925)
                     /locus_tag="PLH57_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02729.1"
                     /translation="MKRKILLTAVLATWLVGSNAFASIARLAVLGTQPSFTAANSSVA
                     TNVVNGSLWYDDSYNMFYNPSAIMDYKDYVTFQKGNSTSGNGGGEAGWVSSVARSFAY
                     GIYVNRGGSAAAAYGDTARLVAPGINSATNTAFAGTSANALNTQRAIDLLFGGDTGIK
                     WGGKLTWAYNRNQSIATQAQDDLEATTRYWHLALGAQVMGFEPFIGTTISSKFHRNNT
                     SDKTLQATDLVDDMNVGVRYKYEAWTPYVTYRKFRRAGDGVGVNDPETSSGSTQVQTS
                     MNVLGLGVGHDMKIADGVHLLKNVGVYYNSVVDTAGATYTNRDYKDYVLPVNFAIEAD
                     AASWLTLRAGAEYHLWNERKFASNGTNSTTNATAAKDKKTSQAGISSVRIGSTFKFGK
                     LHIDSAFGNGATTNQTDQTQAIGFDANTFAFVSASYNW"
BASE COUNT          589 a          527 c          484 g          542 t
ORIGIN      
        1 ctggactccc tcaacaaaaa agtcggggag ctcgactttg ctcaaggtga ctacgaaata
       61 acccttacgt acgtcagtgg gtacgatgaa ctatttgcag atctggtagc ggtaattctc
      121 acggaagatc ctaaggcaat tgaaaaatct gtggatttcg cgggcatgcg ttcttacgtg
      181 agccattcac ggtttgacca gtgggccaaa cacgatgatg cagttagcat gcgcctttcg
      241 accaaacagg aacgggcacg cctatctggc cggttaaatg ctcggcgttt ctcgagtcga
      301 ccgggcgacg gcatcgcgga aatgggtgcc attgtttctc ctcacgagct attcgccgga
      361 acacgggcat ttttgggtaa gtactatttg agtcatcata attaccgtaa caaccccaag
      421 gtgatactcg aaaaggtttt tgatgcgatt gtgcgccaaa taaaggtgct tcagagcaac
      481 cctaaaaaat tcgctgagaa aacactaaaa gaaatcaacc aggatctaaa cgaaacaatc
      541 gaagagcttc taccccgtta aaaaagaaaa gggcccttgg tcgaaaccaa gagccccaac
      601 tttttcgctt attcggcgaa gcttgactgg aattaccagt tgtagcttgc gcttacgaaa
      661 gcaaaggtat tcgcatcaaa accaattgct tgagtttgat cagtttggtt agtggtagca
      721 ccgtttccga aagcggaatc gatgtgcaac ttgccaaact tgaacgtcga accgatgcga
      781 acgcttgaaa tcccagcttg gctggttttc ttgtctttcg cagcagtagc attggtagtc
      841 gagttagttc cattggatgc gaacttacgc tcgttccaaa ggtggtactc ggcgcctgca
      901 cgaagcgtca gccaactagc agcgtctgct tcaatcgcaa agtttacagg caacacgtag
      961 tctttgtaat cgcggtttgt ataagtagcg ccggcagtat ctacaacaga gttgtaataa
     1021 acgcctacgt tcttaagcaa gtgcacgcca tcagcaattt tcatatcgtg accaacacca
     1081 agaccgagaa cattcatgct cgtttgaact tgagtgcttc ccgacgaggt ttctggatcg
     1141 tttacgccaa cgccgtcacc agcacgacga aacttgcgat aagtaacata tggagtccac
     1201 gcttcatatt tgtagcgaac accaacgttc atatcgtcaa ccaagtcagt tgcttgcaag
     1261 gttttgtcag acgtgttgtt tctatgaaac ttcgacgaaa tggtcgttcc gatgaatggc
     1321 tcaaagccca ttacttgcgc gcccaaagca aggtgccagt aacgagtcgt agcttcaagg
     1381 tcatcttgtg cttgagtagc gattgattgg ttgcggttgt acgcccaagt caatttacca
     1441 ccccacttga tgccagtgtc accaccgaac aacaagtcga tcgcgcgttg ggtgttcaat
     1501 gcgttagcac tggttcccgc gaacgccgta ttcgtagccg agttgatgcc cggagcaacg
     1561 agacgagctg tatcaccgta cgctgcagca gcactgccac cacggtttac ataaataccg
     1621 taagcaaagc ttcttgcaac tgacgaaacc caaccggctt caccaccacc gttaccggaa
     1681 gtgctgttac ctttttggaa ggttacgtag tctttatagt ccataatagc tgacgggtta
     1741 taaaacatat tataactgtc gtcataccag agtgaaccgt taaccacgtt agtggctacg
     1801 ctgctgttcg cagctgtaaa cgacggctga gtaccaagta ccgccaatcg cgcaatacta
     1861 gcgaaagcgt tgcttccaac aagccacgtc gctaacactg ctgttaataa tatcttgcgt
     1921 ttcatgcttc ctcctcttaa ctaggccaag ttttctggct attgctttaa gccctaagca
     1981 ttaaagcatt attctccaaa cttctttgcc caaaacaaaa gctgtctact ctaaaagaac
     2041 cacactaaaa gcagaagcta actaagtttg tacggctaat agtttactga caatatctag
     2101 aatcacctcg actacgaagg gtgtcaagaa aattgcagca ct
//
LOCUS       DAOMXO010000425         2140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30097_length_2140_cov_6.216307, whole genome shotgun sequence.
ACCESSION   DAOMXO010000425 DAOMXO010000000
VERSION     DAOMXO010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30097_length_2140_cov_6.216307"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLH57_08705"
     CDS             complement(<1..712)
                     /locus_tag="PLH57_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02730.1"
                     /translation="MKIELILRKKVFWLCILLVLVVARIVTTHTQLSQTSRVNAINKR
                     AAEVNVSSPRLSTANTIDAYSSRDESESVHYESDALYTPKKTANEIIDQFLIHSQPFL
                     NARKKMILSPGDVTYLARAAQDRVLMADSAEVLNTSQLDRNSEHARFLAVDYLSFALR
                     LTHGNDRFLLQLVDDLISNPLEGDVKNARLQKSKLGDRIELFKTLYETDPKRAKEVLS
                     KLKNTQPRVEKFLQSVVQV"
     gene            complement(709..>2140)
                     /locus_tag="PLH57_08710"
     CDS             complement(709..>2140)
                     /locus_tag="PLH57_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02731.1"
                     /translation="DDRSRFFSIAATNPDESALSELQRVSLGAYRAYLKHNSSSLVQA
                     TYTFGSLAGCAEKCFIMQTLATNSTFVPSRYTVKLSRMYVLGNLEREVIYIFEPKQSQ
                     PAAVLALNAGQGVSFIMLRDDEGSSYTFYDRSWTQLGTHSMNSAVQSNRPSKITYNSP
                     SGRDTNLQPPLMGVCQHRWNMSLLKQAGFLDQLTLADYSPGQNLAYRDPRTNLFALGP
                     DGYTWWTHQSAMLEKIVAENKSLTLLPMTETQCFSTNSFLRWAPSATHDGLRTVLVMN
                     SQEMTREICSSKYHKALSDPNIVWVVDAGSNGERNPSRLRCPQNALANTGTGLVVAAS
                     DNHEIDRYSNFGNEYADIAAPKGGEWSVYESFAAVTVARVVAEIGRAFPELDARSLRM
                     AILLGAHIPNAEQPLDVRAGGILDEQRSHQIAMYLETARRQNKDVTDRDTTKTVLQNV
                     FCGSSVQECHIAAVKLKLLLDAGVIK"
BASE COUNT          533 a          482 c          507 g          618 t
ORIGIN      
        1 ttacttgaac aacgctttgc aaaaactttt cgactcttgg ttgtgtgttc ttaagtttac
       61 tcaaaacctc ttttgctcgt tttggatctg tttcatagag ggttttaaat aactcaatac
      121 ggtctcccag tttcgatttt tgaaggcgcg cgttcttaac gtcgccctca agtgggtttg
      181 atattaaatc gtcgacaagc tgtaatagaa accgatcgtt accatgcgtc agccgtagcg
      241 cgaagctaag atagtcaacc gcaagaaaac gagcatgctc tgagttccga tctaattgag
      301 aagtgttcag tacttctgcg gaatcggcca taagtacccg atcctgtgct gcgcgcgcga
      361 ggtatgttac gtccccagga gacaatatca ttttttttct cgcgttcagg aatggctgag
      421 aatgtattaa aaattgatcg atgatttcat tggcagtttt ctttggtgta tacagcgcgt
      481 cggattcata gtgaaccgat tcagattcat cgcgagatga gtatgcatcg attgtatttg
      541 cggtggacaa ccgtggtgaa cttacattga cttcagctgc tcgcttattt atcgcattta
      601 cacgagacgt ttgtgatagt tgtgtgtgtg ttgttacaat tcgcgcgacc accaacacga
      661 gtagaatgca tagccagaat acttttttgc gcaagattaa ttcgattttc atttaatcac
      721 ccctgcatcc aagagcaact tcaacttaac agcagcaata tgacattctt gcacactgga
      781 accacagaat acgttttgca gcaccgtttt tgttgtgtct ctatcggtaa cgtctttatt
      841 ttggcgtcgc gcagtttcga ggtacattgc tatttgatgc gatctttgtt catcaagaat
      901 acctccagcg cgaacgtcga gtggttgttc agcgttggga atatgtgcgc caaggaggat
      961 cgccattcgc agcgagcgag catctaattc gggaaacgcc ctgcctattt ctgccactac
     1021 gcgggctact gtcacagcag caaatgattc gtacactgac cactcgcccc cttttggcgc
     1081 agcgatgtcg gcatattcgt ttccgaagtt tgagtaccta tcgatttcgt gattgtctga
     1141 agccgcgacg actaaaccgg ttccagtatt cgcaagggca ttttgggggc atcgaagcct
     1201 cgatggattt cgctcgccat tactaccggc atctactacc caaactatgt tcgggtcgct
     1261 caaagccttg tgatatttcg aactacagat ttcccgtgtc atttcctggg agttcattac
     1321 gagcacggta cggagtccgt catgggtcgc gctcggtgcc cacctgagaa atgagtttgt
     1381 agaaaaacat tgagtttcgg tcattggaag aagagtgagg ctcttattct cggctacgat
     1441 tttttctaac atggcggatt ggtgcgtcca ccaagtgtag ccatctggcc ccagtgcaaa
     1501 caaatttgtc ctagggtcgc ggtaggctaa attttgacct ggggagtaat cagccaatgt
     1561 cagttgatct aagaaacccg cttgttttaa taaggacata ttccagcgat gctgacagac
     1621 gcccatcaac ggaggttgga gattcgtatc tcttcctgat gggctgttgt atgtgatttt
     1681 gctaggccga ttactttgta cggcactatt catactgtga gtacccaact gggtccaaga
     1741 gcgatcgtaa aacgtatagg aactcccttc gtcgtcacgt aacattatga atgaaacccc
     1801 ctgcccggcg ttaagggcaa gaacggcggc aggttgtgat tgttttggtt caaaaatata
     1861 tataacttcg cgttcaagat tacctaaaac gtacattcgt gaaagcttca cggtatatcg
     1921 cgagggtaca aacgtagaat tcgtcgccag cgtctgcatg ataaaacact tttccgcgca
     1981 tccagcgaga gaaccaaaag tgtacgtagc ttgtacaagt gaactgctgt tgtgttttaa
     2041 gtaggcgcga taggcaccca acgaaacacg ttgaagctct gaaagtgcgc tttcatcggg
     2101 gtttgttgct gcaatagaaa aaaacctcga tcgatcgtca
//
LOCUS       DAOMXO010000426         2135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30199_length_2135_cov_3.025000, whole genome shotgun sequence.
ACCESSION   DAOMXO010000426 DAOMXO010000000
VERSION     DAOMXO010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30199_length_2135_cov_3.025000"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PLH57_08715"
     CDS             <1..916
                     /locus_tag="PLH57_08715"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRK02732.1"
                     /translation="LTLLTQSAAAVDSKNAQNPTSELRVVTYNIFYGKALDLERKLYP
                     AFLKKRVSIARFLNRHSALKNFDILGLQEICEGRPGSASDQQEYFREILRAQGISPHG
                     RFVAANPRSNDRCRSGQMILSRFPIVESGTFELPLLRPGGKQALWVDIEVPTVGGTKR
                     VRVDIEVPTVGGTKRVRVDNLHLDNRGKSLLAEKGRWKQIQTVLTHLEHWQSKNPDTP
                     VIVLGDFNALNKLYNPWKREKTIREMSKLFQPSLKKYKATHITGHQLDWIFSRNLKLK
                     KSRVAYKMLSDHFPVSADFEIKTMPSNP"
     gene            complement(891..1298)
                     /locus_tag="PLH57_08720"
     CDS             complement(891..1298)
                     /locus_tag="PLH57_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRK02733.1"
                     /translation="MLIKKTIVAGLFSLFFFSVLGLQVEAKTDCSDDSRYGEIEKSEL
                     QAKIKDGKVSLIDVNDEETFAKNKIGNAINYYEKKNDLAWVLPKDKSALVVAYCGSTR
                     CTAWKKAAKAACEMGYTNVKHFKPGIKGWMASS"
     gene            complement(1329..>2135)
                     /locus_tag="PLH57_08725"
     CDS             complement(1329..>2135)
                     /locus_tag="PLH57_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02734.1"
                     /translation="FRQAEANSINGVVEGALDDATAFSRFGLDLLERAYERDKTPFKL
                     AGNRWLGFQGVSDEDARVVEIDQLDAVAAAKYMRNRFMPLGHLVILTDPLTKYGGRFD
                     LPQFDEIREAAHFLEASAEDMTLREVETNPDLAAFRGLPEEDLPKRRSQLEKIFLHKF
                     GHLDYLVNGLPGGHPWPDASDRESQARLRAYRDFIAPGKTFVEIYLDHLNRSNGWRSK
                     RIHVTLEDIMNAKPGRKTKAEYFSEVYTMWFVKEPQRVLQIYQEMGVIPK"
BASE COUNT          554 a          518 c          506 g          557 t
ORIGIN      
        1 gctgactttg ctgacgcaaa gcgccgctgc ggttgattcc aaaaatgcgc aaaacccaac
       61 ctcggaattg cgagtggtca cctacaatat cttctacggt aaagcgctcg atctagagcg
      121 gaaattgtac ccggcgtttc tcaaaaaaag agtgtcgatc gcgaggtttt taaaccgtca
      181 ttcagcgcta aagaactttg atatattggg gttacaggaa atctgtgagg gccgccccgg
      241 aagcgcgagc gatcagcaag agtattttcg ggaaattcta agagcccagg gaatcagccc
      301 gcacgggcga tttgttgcgg caaatccacg atctaacgat cgatgtcgtt ccggtcaaat
      361 gatattgagt cgcttcccga ttgtggaatc gggaaccttc gagcttccat tgttgcgccc
      421 cggtggcaaa caagcgcttt gggtggatat cgaagtgccg accgtcggcg gaaccaaacg
      481 cgtgcgggtg gatatcgaag tgccgaccgt cggcggaacc aaacgcgtgc gggtggataa
      541 tctgcacctc gacaaccgag gaaagagctt attggccgaa aaaggccgct ggaaacaaat
      601 tcagaccgtg ttgacacacc tggaacattg gcaaagcaaa aatcccgata cccctgtcat
      661 tgttttaggg gattttaatg cactaaataa gctctacaat ccatggaagc gcgaaaaaac
      721 tattcgcgag atgagtaaat tatttcagcc gtccctaaaa aaatacaaag ccacccatat
      781 tacgggtcac caattagact ggatcttctc gagaaatcta aaactcaaaa agtcccgcgt
      841 ggcctataaa atgctttcgg atcacttccc cgtatcggcg gatttcgaaa tcaagacgat
      901 gccatccaac ccttaattcc gggtttaaaa tgtttgacgt tggtgtagcc catttcgcaa
      961 gcggctttag cagccttttt ccaagccgtg caacgggtgc tgccacaata ggctacgact
     1021 agtgctgatt tatcttttgg cagaacccaa gcgagatcgt ttttcttttc gtaataatta
     1081 atggcattac caattttatt tttggcaaaa gtctcttcgt cgttgacgtc tatgagtgag
     1141 actttcccgt cttttatttt tgcctgtaat tccgattttt caatttcacc gtatctagaa
     1201 tcatcgctac agtccgtttt tgcttcgact tgtagtccga gaacggaaaa aaagaataat
     1261 gaaaataatc ccgcaacaat agttttttta atcaacatca gagtcccccc gggtgagata
     1321 aaattttact atttcggaat tacacccatc tcctggtaaa tctgaagcac gcgctgcggc
     1381 tcttttacga accacatggt gtaaacctcc gaaaagtact cagccttggt tttgcgacca
     1441 ggcttcgcgt tcatgatatc ttccaaggta acgtggattc gcttacttct ccagccgttt
     1501 gatcgattta gatgatccaa gtaaatctca acaaatgttt tacccggtgc aataaagtct
     1561 cgataggcgc gaaggcgggc ctgactttcg cggtcacttg cgtcgggcca tgggtgtccg
     1621 ccgggaaggc cgttgaccag gtagtccaag tggccaaatt tatgtaaaaa tattttctcc
     1681 aattgagagc ggcgttttgg caggtcctct tcggggagac ctctgaaggc cgctaaatct
     1741 ggattggttt ctacttctcg caaagtcatg tcttccgccg acgcttcgag aaagtgcgca
     1801 gcttctcgaa tttcgtcgaa ctgtggaaga tcaaatctac cgccatattt agtcagcgga
     1861 tcggttaaga tcaccagatg ccccagtggc ataaaacggt tgcgcatgta tttggctgcg
     1921 gcgacggcat ctaactggtc gatttcgacg acgcgggcat cttcgtcgga tacaccctgg
     1981 aatccgagcc agcggtttcc ggccaatttg aagggagttt tatctctttc gtaagctcgc
     2041 tccagcaaat cgagtccaaa gcgactgaag gcagttgcat cgtcgagtgc cccttctacg
     2101 accccattaa tagagttagc ttcagcttgc cgaaa
//
LOCUS       DAOMXO010000427         2130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30293_length_2130_cov_4.414940, whole genome shotgun sequence.
ACCESSION   DAOMXO010000427 DAOMXO010000000
VERSION     DAOMXO010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30293_length_2130_cov_4.414940"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /gene="purD"
                     /locus_tag="PLH57_08730"
     CDS             <1..1114
                     /gene="purD"
                     /locus_tag="PLH57_08730"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRK02735.1"
                     /translation="GPDDLLAQGVGDFLEKKDFVVFGPKKAAAKLEWSKAFAKEMMQT
                     CGVPTAESVELTAADLDTFESKLETLLPYPVVLKYDGLALGKGVVIAQNTDEARSFLK
                     EVFEEKKFERVGFESDTKAAAPVVLAEEFLTGFEVSLFAICDGKDYALLQPACDYKKL
                     KTGNRGPNTGGMGAYSPVPWLPDARCREIADKIFKPILDEMRARKVTFRGLLYAGLMV
                     RGQDFWVLEFNVRFGDPETQALLPRLSSDLALVLYGAALGELEKALDLAPLRWKSEAC
                     VNIVAASRGYPEKPETGFEVHFDGFRPSEQQRLFFAGVRNLDGKIQTSGGRVFGISQL
                     GPTIETALDAALVAMSQVGFEGMYFRSDIGRIGDDA"
     gene            1111..1746
                     /gene="purN"
                     /locus_tag="PLH57_08735"
     CDS             1111..1746
                     /gene="purN"
                     /locus_tag="PLH57_08735"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRK02736.1"
                     /translation="MSAIPRVAVFASGGGSNFKALIEHQKTGKLRAQIVGLITDQPKA
                     GALEFARAQKIANAVHAESDRKVREQNILATLQEWKPDWLVLAGFMRLLSPEFLNHFR
                     DKIQECFRVVNIHPSLLPAFPGRDSYRQAFDHGVATTGVTVHFVSSGVDDGPIIAQAP
                     LQILPHDTVDSLKARGLALEHIIYSATLERLWSEKWELRPTASGRRRLHWL"
     gene            1737..>2130
                     /locus_tag="PLH57_08740"
     CDS             1737..>2130
                     /locus_tag="PLH57_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02737.1"
                     /translation="MAVISRLRVERTVAAEEFDAVAHRIVQEIPLYFPAWKDLKVQCR
                     STELFWTCAKVPRSELETAAKLALASSLYVVTSDQFTFAPHAASAGREWCLEKHFRFG
                     VTDNSGNVLAEALALASPTPLADVRARSG"
BASE COUNT          503 a          485 c          608 g          534 t
ORIGIN      
        1 aggtcccgac gacttgttgg ctcaaggtgt gggggatttt ctcgaaaaga aagatttcgt
       61 ggtctttggt cctaaaaaag cggcggcgaa acttgaatgg agtaaagcgt ttgcaaaaga
      121 aatgatgcaa acttgcggcg taccgacggc agagtccgta gaacttacgg ccgccgatct
      181 tgatacgttt gaaagcaaac ttgagacgtt gttgccgtac ccggtggtgt taaaatacga
      241 tggtttagca ctaggaaaag gcgtagttat tgcgcaaaac accgacgaag cgcgatcttt
      301 tctcaaggaa gtgttcgaag aaaaaaaatt cgaacgtgtg ggttttgagt ctgataccaa
      361 agctgccgca ccagtggtat tggccgaaga attccttacc ggattcgagg ttagtttgtt
      421 cgcaatttgt gacggcaaag attacgcgct gttgcaacct gcgtgtgact acaaaaagtt
      481 aaaaactggc aatcgaggtc ccaacactgg cggcatggga gcctacagcc ctgttccctg
      541 gttgcccgat gcgcgctgcc gggagatcgc agacaaaatt tttaaaccga tattagatga
      601 aatgcgggca cgcaaagtca cctttcgcgg cctactgtat gccggtctga tggttcgggg
      661 ccaagacttc tgggtgctag aattcaatgt gcgatttgga gatccggaaa cgcaggcgct
      721 attgccgcga cttagttcgg acctagctct cgtactctac ggtgctgcac tgggagaact
      781 cgaaaaagct ctcgacctag cgcctcttcg ttggaaaagc gaagcttgtg tgaatattgt
      841 tgctgcgagt cgcggttatc ccgaaaaacc tgaaacgggt ttcgaagttc atttcgacgg
      901 gtttcgtcct agcgaacagc agcgattgtt ttttgctggt gtgcgaaatt tggacggtaa
      961 aatccaaact tcgggtggac gcgtgtttgg aatttcgcaa ttgggcccca cgatcgagac
     1021 cgcgcttgat gccgccttag tggctatgtc tcaggtcgga ttcgaaggaa tgtattttcg
     1081 cagcgatatc ggacgaattg gagacgatgc atgagtgcga ttccaagggt ggcggtgttt
     1141 gcgagcggtg ggggatcgaa ctttaaggcg ttgatcgagc atcaaaaaac aggaaagttg
     1201 cgggcccaaa ttgttggact cattacggat cagccgaaag caggggcttt ggagtttgcg
     1261 cgcgcgcaaa aaattgcaaa cgcggtgcat gcggagtctg atcgtaaggt gcgagaacag
     1321 aatattctag cgactcttca agagtggaaa ccagactggt tggtattagc tgggtttatg
     1381 cgactactca gtccggaatt tttgaatcat tttcgcgata agattcaaga gtgttttcgg
     1441 gtagtcaata tccacccgtc gcttttgccc gcgttcccag ggcgtgactc ctaccgacag
     1501 gcgtttgatc atggagtggc aaccaccgga gttacagttc attttgtaag ctcgggagtc
     1561 gacgatgggc ccatcatagc ccaagcaccc cttcaaatct tgccacacga cactgttgat
     1621 tcgctcaagg cgcgcggatt ggccctggaa catatcattt attcggccac tctcgagcgt
     1681 ttgtggagcg aaaagtggga attacgcccc acggctagtg ggcggcggag gctccattgg
     1741 ctgtgatatc gagacttcga gttgaacgca ccgtggctgc cgaagagttc gatgctgtcg
     1801 ctcatcggat cgttcaagaa attccgctct attttccggc ttggaaagac ctcaaggttc
     1861 aatgtcgatc gaccgaattg ttttggacct gtgcgaaagt tccgcgctct gaacttgaaa
     1921 cagccgcgaa attggctctg gcgtcctcgc tctacgtggt gacgagtgat caatttactt
     1981 ttgcgcctca tgccgcctcc gcagggcggg agtggtgttt agaaaaacat tttcgttttg
     2041 gcgtaactga taattcaggc aacgtgttag ccgaagcgtt agcgctggcc agtccgactc
     2101 cgttggccga cgtgcgggct cgctctggac
//
LOCUS       DAOMXO010000428         2124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30415_length_2124_cov_4.647173, whole genome shotgun sequence.
ACCESSION   DAOMXO010000428 DAOMXO010000000
VERSION     DAOMXO010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30415_length_2124_cov_4.647173"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1943)
                     /gene="pnp"
                     /locus_tag="PLH57_08745"
     CDS             complement(<1..1943)
                     /gene="pnp"
                     /locus_tag="PLH57_08745"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRK02738.1"
                     /translation="MFNPVTVSCDFGGRKLTIETGRLAKQADGSVLVTYGDTVVLVTA
                     VSKSDMKDGIDFFPLTVEYQEKFYAAGRIPGGFFKREGRPSSDATLTARLIDRPIRPL
                     FPEGFFYDTQVIATVLSVDKENDPDVVASVGASAALAISSIPFQGPTAACRVGRVNGQ
                     LICNPTIAQQEESDMEIFVASTKSAIMMVEGGARVVPEEEVLQAILFGQKAMQPVIDI
                     IESLKSKVGKTKRTFEPKKVEDTFKKSAEKIMLPLVLEALATQKKEDRYGLYDKAYDT
                     AEAQLIKDGDENASERKKELEGILENLKYVQMREMILSKKSRIDGRNLVTVRPIAVEA
                     HLLPRVHGSALFTRGETQVLAAVTLGTGDDVQMIDGMRGSYDKKFMLNYNFPPFSVGE
                     TGRVGSPGRREIGHGALAERALVNVMPKDCPYTVRVVCEVLESNGSSSMGSVCSGSMA
                     LMDAGIEFKKPVAGIAMGLIKEGSRFAVLSDILGDEDHLGDMDFKVAGTDEGVTAIQM
                     DIKIEGVNEEIMRTALKQAHEGRMHILSKMAPAIEAPRAAVSDYAPTFTTFKIKPDKI
                     RDVIGSGGKVIKGIIEQTGVKIDVEDDGTIKIASTDPVQGRKAEDIIRNIVAEPEVGR
                     TYTGTVRSIVEFGAFVEIIPGTDG"
BASE COUNT          491 a          567 c          489 g          577 t
ORIGIN      
        1 ccatcggtac caggaataat ttcgacaaac gcgccgaact ccacgatcga acggaccgtg
       61 ccggtatagg tgcgaccaac ctctggttcc gctacgatat tgcgaataat gtcttcagcc
      121 ttacgcccct gtactgggtc ggttgaagca atcttgatgg tgccgtcatc ttctacgtcg
      181 atcttaacgc cggtctgctc gatgatacct ttgatcacct tgccaccact accgatcaca
      241 tcccgaatct tatcgggctt gatcttgaac gtggtgaacg tcggagcata gtccgaaacc
      301 gccgcgcgag gagcttcgat tgccggtgcc attttcgaaa gtatgtgcat gcggccctcg
      361 tgcgcttgtt tgagcgcggt acgcatgatt tcttcgttaa cgccttcaat tttaatatcc
      421 atttgaattg cggtcacgcc ttcgtcagta cccgcgactt tgaagtccat gtcgcccagg
      481 tggtcttcgt cccccaaaat gtcgctcaat accgcaaagc gcgagccttc tttaataagg
      541 cccatggcaa ttccagccac tggttttttg aattcaatgc ccgcgtccat aagcgccatg
      601 gatccagagc acaccgaacc catcgaacta gaaccgttac tttcgagcac ttcgcaaact
      661 acccgcacag tgtaggggca gtcttttggc atcacgttta ctaatgcgcg ctcagccaac
      721 gctccgtggc caatttcgcg gcgtccaggc gaacctactc ggcccgtctc gccaacgcta
      781 aatggaggaa agttataatt gagcatgaac ttcttgtcat acgacccgcg catgccatcg
      841 atcatttgca catcatcgcc cgttccaagc gttactgcag caagcacctg tgtctcgccg
      901 cgcgtaaaaa gtgccgaacc gtgaacgcgt gggagcaagt gtgcttccac tgcgatcgga
      961 cgaacggtaa ctagattacg tccgtcgatg cgcgattttt tcgaaagaat catttcgcgc
     1021 atttgaacgt atttaagatt ctcgagaatt ccttcgagtt cttttttccg ttcagaggcg
     1081 ttctcatcgc cgtctttaat gagctgggct tcagccgtat cgtaggcttt atcgtaaagc
     1141 ccgtatcgat cttctttttt ctgggtagca agcgcttcga gcactagagg aagcatgatc
     1201 ttctcggccg atttcttaaa cgtatcttca acttttttcg gttcgaaagt tcgtttcgtt
     1261 ttgcccactt ttgattttag tgactcgatg atgtcgatca caggctgcat agctttttgt
     1321 ccaaaaagaa tggcttgtaa cacttcttct tctggcacaa ctcgcgcgcc gccttcgacc
     1381 atcataatgg cgctcttagt gctagctacg aaaatctcca tgtcggattc ttcttgctgc
     1441 gcaatcgtgg gattgcagat caattggcca ttaacgcgac caacgcgaca agcagccgtt
     1501 ggaccctgaa acggaatcga ggaaatcgca agtgcggccg aagcgcctac gctcgcaaca
     1561 acgtccgggt cattttcttt atcgacactc agtactgttg caatcacttg cgtgtcatag
     1621 aaaaagcctt ctggaaacaa cgggcgaatt gggcgatcaa tgagccgtgc agtcaatgtt
     1681 gcgtcggatg aaggtctccc ctcgcgttta aagaagccac ctggaattct accggcagca
     1741 taaaattttt cttggtattc tacagtcagc ggaaaaaaat cgattccgtc tttcatgtca
     1801 cttttgctca ctgcggtgac aagcaccaca gtgtcgccgt aagtcacgag taccgaacca
     1861 tctgcctgtt ttgccaagcg gccggtttcg atagtgagtt tgcgtccgcc gaaatcgcaa
     1921 gacacggtta ctggattaaa cataattgat cactccttgt ttttgtatga tgagagcgta
     1981 ggagggttta tgaaacctga gtcgcgacca ctgtcttgtg gtcgaaccac gaatcggaaa
     2041 aatagatttt tagttaaatc tttttttcca attcgtggtt tctatcaaac ccctaaaaaa
     2101 aagggccctt tcgggccctc tctc
//
LOCUS       DAOMXO010000429         2123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30443_length_2123_cov_4.585106, whole genome shotgun sequence.
ACCESSION   DAOMXO010000429 DAOMXO010000000
VERSION     DAOMXO010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30443_length_2123_cov_4.585106"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLH57_08750"
     CDS             <1..418
                     /locus_tag="PLH57_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02739.1"
                     /translation="TVGGLVLQSPWFSAIFALVVFAAIGFPGIGLFGPRTLIFLGAFK
                     ERAVLTLVGLFGILLTAGFLMGAYRRVFLGKPGHDSERVFDLSLGEKAIVLPMVALII
                     FIGVSPGYLIRVSENTVAKLVSALHAPEESKTDVQQ"
     gene            381..1889
                     /locus_tag="PLH57_08755"
     CDS             381..1889
                     /locus_tag="PLH57_08755"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRK02740.1"
                     /translation="MRQKKVRQMFNSDLSQNLSVLPEAIILLTAVLVILADTFFAYKD
                     NSTRELSGFLTFIGIFAALVVLVMTWNLAPSRLAQDSLMIDPVSQFLKAMAVLCGLAT
                     VPMVASTKEIRIETRGEFYALLLLMLFSSMIIVSAQNLLAAYVAIQMCILSATLLTAF
                     RARPKTSSEAGLKYFLHSQIVLAVFLFGMALLFFHTDSLYIPEIRAQLESRGTAAGFS
                     LVTFSFLALCPLVLAWVFPFQMLAPDASQGAPTVFSGFILAVPFISGLSLLLKFCVQL
                     FGVPQDGGWAPIPSVRWDLLFSILSALTISVGTLGSVRQSNVKRFLAYCAVAQVGYIL
                     LGMAVLDQRAIVAILSGVTSFGVALFGIFAVTHRIIESSQGESFESFRGLVWRDGLIG
                     VAACIFLLSLAGLPPFAGFASRFELLGAVVHGKLYWLAGVVVLNWVIGLVAYGKLIKE
                     IFYPIRQLEEFRGSANAARFVVIAMLVPTVILGVAWNTLHGALAESVSKLTW"
BASE COUNT          431 a          436 c          568 g          688 t
ORIGIN      
        1 aactgtaggt ggattggtcc tacagtcccc gtggtttagc gcaattttcg cgcttgtggt
       61 gtttgccgcc atagggtttc ccggcattgg tctttttggc cctcgaactt tgattttctt
      121 aggagcgttt aaggagcggg cggttctaac tctagttgga cttttcggca tattgttgac
      181 ggctggattt ttgatgggag cctatcgaag ggtgtttttg ggcaagccag gtcatgactc
      241 ggagagagtt tttgatctga gtcttggtga aaaagccatt gtgttgccaa tggtcgctct
      301 aattattttt atcggggtat cgccaggtta tttaattcgg gtctccgaaa acactgtggc
      361 caaattggta tcagcgctcc atgcgccaga agaaagtaag acagatgttc aacagtgatc
      421 taagtcagaa tttatctgta cttccagagg caattattct gctcacggca gttcttgtga
      481 tattggcgga tacgtttttt gcttataagg ataattcgac acgggaactt tcgggctttt
      541 tgacctttat cggaattttt gctgcgcttg tggttttggt aatgacctgg aatttggcgc
      601 caagtcgatt ggcccaggat tcattaatga tcgacccagt tagtcagttc ctaaaggcga
      661 tggcggtgtt gtgcggtttg gccaccgtac ccatggtggc ttcgactaaa gagattcgaa
      721 ttgaaacccg aggcgaattt tacgcattac ttctgctgat gctattttcg tctatgatca
      781 tcgtgtcggc gcaaaaccta ttggccgctt acgtagccat tcaaatgtgc atattgtccg
      841 caactttgct gacggcattt agagcgcggc cgaaaacctc cagcgaagca ggattaaaat
      901 actttcttca ctcacagatc gttttggccg tttttctttt tggcatggct ttgttgtttt
      961 ttcataccga ctccctctat attcctgaaa tccgcgctca gctcgagtcg cgcggcacgg
     1021 cagcggggtt ttcgttggtt acattttcct ttctggcgct gtgcccgttg gtattggctt
     1081 gggtgttccc gttccaaatg ctcgctccag atgcaagcca gggcgcgccg acggtgtttt
     1141 cgggatttat cttggcagta ccctttatta gtggactttc gttactgcta aaattttgtg
     1201 tacaactttt tggagttccc caggatggcg gatgggcgcc tattccgagt gtgcgctggg
     1261 atctgctctt ttcaattctc agtgctttaa caattagcgt ggggactttg ggttcggtta
     1321 ggcagtccaa cgtgaaaagg tttttggctt actgtgcagt agctcaagtg ggttacattc
     1381 ttctcggaat ggcagtgttg gaccaaaggg cgattgtggc gatcctttca ggagtaacta
     1441 gttttggggt ggcgcttttt gggatcttcg cggttacgca ccgcataatt gaatccagtc
     1501 agggcgagtc cttcgagtct tttagagggt tagtttggcg tgacggtctt atcggagtag
     1561 ctgcatgtat ttttttacta agtttggccg ggttgccgcc attcgcgggg ttcgccagcc
     1621 gttttgaact gctgggagcg gttgttcacg gaaagctgta ttggctggcc ggcgttgtgg
     1681 tcctgaattg ggtgattggt ttggttgctt atgggaagct gattaaagaa atattttatc
     1741 ccattcgcca gctcgaggaa tttcgcgggt ccgccaacgc tgcccgattc gttgttatcg
     1801 ccatgttggt acccaccgtg attttgggag tggcgtggaa tacccttcac ggagcgttgg
     1861 cagaatctgt ttcgaagcta acttggtaaa aatcgagttt tttgtattta ttttgtacgc
     1921 gaacaaaaat ataccaagtt catccattta tttataatat ccatctaacg atcataaatt
     1981 ttttggaaag gggttcgatg tgagtgcata cgtgcccgtt ttaattttaa tgggtgtcgg
     2041 catgacaatc gctggagtcg ttctggtgat cacccatatt tttggaccga agaacccgaa
     2101 cgttctgaaa atggacgtat atg
//
LOCUS       DAOMXO010000430         2119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30525_length_2119_cov_4.192345, whole genome shotgun sequence.
ACCESSION   DAOMXO010000430 DAOMXO010000000
VERSION     DAOMXO010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30525_length_2119_cov_4.192345"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..634
                     /locus_tag="PLH57_08760"
     CDS             101..634
                     /locus_tag="PLH57_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRK02741.1"
                     /translation="MDHVLVLNASFEPIVVIHWQKAMQLLFQGKVEVLEEYEREIRTV
                     SLTFRLPSVLRLIKYIPIARKKNIVRFSRANIFVRDGFCCQYCGRKRARHELTLDHIV
                     PVVQGGRKTWENIVTACIQCNQRKGGRTPHEAGMRLICRPAEPQWLPTFTIRYRLNSA
                     PETWKLYFSWNIQTGSA"
     gene            607..2076
                     /locus_tag="PLH57_08765"
     CDS             607..2076
                     /locus_tag="PLH57_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02742.1"
                     /translation="MEHSNRLSIEGRDKQLPSFAERVYKAHFGFLKEALETFVPPEAW
                     NEVKVSYARRIRVEGYEWSNGKYTHLGVYGVLFQVLLSRTDLLEYLDLPFERGYLLWL
                     RDRYDLSPRDLGAAVRCSEGTVRTRILQSRKTTLERLRSLEPIELNFSRKSNKTTHHY
                     CIQSQEIVENWTPVISATGPLRTNFSVDLPAVVAESLDSNTGSCEGCVNYAEARARGY
                     IYAQKRQNLNLTSDRSQIGTAEFEANQADSESDFPIRAILEKKEHSLSLNWEAAPWYL
                     KIIIEGFLATSLIVGVVLAVPRIKSLYEYWLEKRFDLYSITEVASNLSSELVDVAQTI
                     SEEKPSESEIYGPADPPTTDSLAVVARTRKELPSSKPTQEKIAIQAETEFGGGESLAT
                     ASSKIFRVFLKTDSPESLSENVLQILSKVEHKAADDAIPLGSQIPGGVLIDVYVPVKN
                     YTSIINEVTKLGDSKLIITSAKGKLVPGKARIRIWIQRI"
BASE COUNT          566 a          508 c          507 g          538 t
ORIGIN      
        1 acttttacaa ccatgtccgg cagacaccct aaataatgca tcgcaccaca tttctttgcc
       61 gatttgaact cggacgaaaa tcttcatata cttggggagt atggaccatg tcttggttct
      121 caatgcgtct ttcgaaccga tcgtagtgat tcactggcaa aaggccatgc agttattgtt
      181 tcagggcaag gtagaggtcc tggaagaata cgagcgggag attcgcacag ttagtcttac
      241 atttcgtctg ccttcggtat tgcgtttaat caaatacatc ccaattgctc gaaaaaagaa
      301 catcgtgcgc ttttcacgcg ccaatatttt cgtacgcgat ggtttttgtt gccagtattg
      361 cggtcgaaag cgggcgcgcc acgagctcac tctcgatcat attgttcccg tggtccaggg
      421 tggccgaaaa acttgggaaa acatcgtcac cgcctgcata caatgcaacc aacgaaaggg
      481 cgggcgcact ccgcacgaag ccggaatgcg tttgatttgc cgtccagcgg agccgcagtg
      541 gttacccact tttacgatcc gttatcgctt gaattccgct cctgaaacct ggaaactgta
      601 tttctcgtgg aacattcaaa ccggctcagc atagagggtc gcgacaaaca actcccgtca
      661 tttgccgaaa gagtttataa ggcgcatttt ggcttcctaa aagaggctct cgagactttc
      721 gtaccgccgg aagcgtggaa tgaagtaaag gtctcgtacg ctcgtcgaat tcgagtcgag
      781 ggttacgagt ggtctaatgg aaagtacacc catttggggg tttacggagt acttttccag
      841 gttttacttt cacgaaccga cttactcgaa tacttagacc taccgtttga acggggctac
      901 cttctatggc tccgagatcg ctacgatctt tcacctcgcg acctcggagc ggccgtacgg
      961 tgttccgaag gaactgttcg aacaagaatt ttgcaatccc gtaaaactac gttggagagg
     1021 cttcgttcgc ttgaaccgat cgaactaaac ttttcaagaa aatccaataa gacgactcat
     1081 cattattgta ttcagtctca agaaatcgtt gaaaactgga cgcccgttat atcagccacc
     1141 ggaccgctac gaacaaattt ttccgtcgac ttaccagcag ttgtagccga gagcctagat
     1201 tcgaatactg gtagctgcga gggttgcgta aactatgccg aggcacgggc tcgcggatac
     1261 atttatgcgc aaaagcggca gaatttaaat ttaacgtctg accgatcaca gattggcacc
     1321 gctgaatttg aggcaaacca ggcggactct gagtcagatt ttccgattcg cgcaatccta
     1381 gagaaaaaag aacattcgct ctctttaaac tgggaagctg cgccttggta tctaaagatc
     1441 attattgagg gttttctcgc aactagccta attgtgggag tggtgctggc agttcctcgc
     1501 atcaaatcac tctacgagta ctggctagaa aaaagattcg acttatacag cataaccgag
     1561 gtggcttcga atctttcgtc ggaactggtt gacgtggccc aaaccatctc agaggaaaag
     1621 ccatcggaaa gtgaaatata cggcccagca gaccctccga cgacggattc actagccgtc
     1681 gtggcgcgaa ccagaaaaga gctcccgtct tccaagccaa cgcaagagaa aatcgccata
     1741 caagcggaga ccgaatttgg tgggggcgag tctcttgcaa cagcttcttc caaaattttc
     1801 agggtatttt tgaaaaccga ctctcccgaa tcgctttcgg aaaatgtttt gcagatttta
     1861 tcaaaggttg aacacaaggc tgccgatgat gcgatcccac tcggatccca gattcctggt
     1921 ggcgtactga ttgacgttta tgttcccgtg aagaattaca cctcgataat caatgaggtc
     1981 actaaactcg gtgactcaaa gctaattatt acatccgcga aggggaaatt agttcccgga
     2041 aaagccagaa tccgaatctg gattcagaga atttagaaat tttcttaata ttgctagggg
     2101 ctggtggggt ccattagtg
//
LOCUS       DAOMXO010000431         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30740_length_2108_cov_3.419873, whole genome shotgun sequence.
ACCESSION   DAOMXO010000431 DAOMXO010000000
VERSION     DAOMXO010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30740_length_2108_cov_3.419873"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1280)
                     /locus_tag="PLH57_08770"
     CDS             complement(54..1280)
                     /locus_tag="PLH57_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRK02743.1"
                     /translation="MGATANKKLIAVLKSLLVVSGIVSWDLGLGAEIKNSMMAASKLN
                     ADAIVHLRSEITSLQRELTESKVRETSSNSQLKKIKKLLSLQAREIQLSEQRILQLNT
                     SLDELSRRRLEVALRIEEQKKIIRKRLLELYKLAGVDHFGDRFKLLEILNADVFAQKS
                     YFLNKVLTRDIASVHKLQGDVQEALALELKILEERNKLDYYVFELKDKSAELTANRQI
                     HQEILKTNRVNHLEALKRIRSLRESEMELEQMLDQFQHKKSETSEPFSLASLKGQLEL
                     PVDGAIVSEFGRSFNPKTNLLTFQKGISIETAANSNVRAVHEGQVVYQGPLKNYGSIV
                     ILEHPGQYFSLYGQLAKTGLKVGDVLRKGELVGATSEAPFYFEIREKNVAVNPVQWIN
                     HQAVANFKNGGKHVVQ"
     gene            complement(1253..>2108)
                     /locus_tag="PLH57_08775"
     CDS             complement(1253..>2108)
                     /locus_tag="PLH57_08775"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRK02744.1"
                     /translation="VLRMTLTSLKHRWGARSVSVLSMTTILVLTGLIFEVITSFSGVI
                     SDLRTSRWMTLYLSPLVSESQELTVLSGVKKISGVQSAQLVSKDAFISNFQRYFPSLA
                     KGLEELELDTIPRYIKVKAEANKLDQIKKIASTDRTIEQIETNEARFAGLIRMLALLK
                     TFCLALLVGLGLAGATLIVHRNQLRNRLQEQLRRTLVDLGARRSLRALPSVLEGMVEG
                     VLAGALAAGILVYAGSRLQKSLAQLFGAMGYESLPVRPLWSLFFILFLGFFSGMIGSV
                     WAQLRIKN"
BASE COUNT          556 a          532 c          450 g          570 t
ORIGIN      
        1 aactcgccat cgctaccgtc gcaactactg ccatccaagg caaacccaaa gacctattgt
       61 acgacatgtt ttcccccgtt tttgaagttc gccaccgctt gatggttgat ccactgtact
      121 ggatttactg ctacgttctt ttctctaatt tcgaaataaa aaggcgcttc cgaagttgcc
      181 cccacgagct ccccttttcg caaaacatct ccaactttta ggccggtttt agcaagctgc
      241 ccatagagtg aaaaatattg cccagggtgc tcgagaatca caatagaacc gtaatttttg
      301 agtggtccct ggtataccac ctggccttcg tgtaccgcgc gcacattgga attggccgca
      361 gtttctatgg atattccttt ttgaaacgtt aacaagttgg ttttgggatt aaacgatcta
      421 ccaaactcgc tcactattgc accgtctacc ggaagttcca gctgaccttt gagtgacgcc
      481 aaggaaaacg gttccgaagt ttcacttttt ttatgttgaa actgatccaa catttgctca
      541 agttccattt cgctttcgcg cagtgaacga attcgcttaa gtgcttctaa gtgatttacc
      601 cggttggtct taagaatttc ctggtgaatt tgtctattcg ccgtaagttc ggccgattta
      661 tctttgagct caaaaacata gtaatcaagt ttattgcgtt cttcgagaat cttaagttca
      721 agagcaagag cttcctgcac gtcgccttgc aacttatgca ccgaagcgat gtctcgagtg
      781 agaaccttgt ttaaaaaata gctcttctga gcgaacacgt cagcgttcaa aatttctaac
      841 aatttgaaac gatcaccgaa atggtcgaca cccgcgagtt tataaagctc caacagtcgt
      901 ttgcgaatta tttttttctg ttcctcaatt cgaagggcca cctctagccg ccgtctcgat
      961 aactcatcaa ggctggtgtt gagctgcaga attcgttgtt cagagagctg aatctcgcgg
     1021 gcttgcaacg agagtagctt tttaattttc tttaattggg aatttgaact tgtctctcga
     1081 accttcgatt cagttaattc tctctgaaga gaagtgattt ccgaacgcag gtgcactatc
     1141 gcgtcggcgt tgagtttaga cgccgccatc atactattct taatttcggc acccaaacct
     1201 aagtcccacg agacaatgcc tgaaactacc aacagtgatt ttaaaaccgc gattaatttt
     1261 ttattcgcag ttgcgcccat acacttccga tcatccctga aaagaagccg agaaacaaaa
     1321 tgaaaaagag tgaccagagg ggtcgcactg gtagcgactc gtaccccatc gcaccaaaaa
     1381 gctgagccaa gcttttttgt agacgacttc ccgcataaac caagattccg gcggccaaag
     1441 ctcccgccaa tacgccttcg accataccct caagcaccga cggcaacgcg cgcagtgagc
     1501 ggcgagcccc caaatctacc agagtccggc gcagctgctc ttgcagacgg ttccggagtt
     1561 gattgcgatg aactatgagg gtagcacccg ccagcccgag tccaacgagt agcgccaaac
     1621 aaaaggtctt cagtagcgct agcatgcgaa tcaacccagc gaatcgcgct tcattggttt
     1681 caatttgctc aatggtgcga tcggtgctgg ctatcttctt aatttgatcg agcttattgg
     1741 cctcggcctt aactttgatg tagcgcggaa tggtatcgag ctctaactct tcaagtcctt
     1801 ttgcaagact tggaaagtaa cgttgaaaat tagaaatgaa cgcgtcttta ctcaccaact
     1861 gtgcactttg cacgccggaa atctttttta ctccgctcaa cacagtcaac tcttggcttt
     1921 ctgagactag aggagaaaga taaagcgtca tccaacgcga agttcgtaaa tcggaaataa
     1981 cgccggaaaa gctcgtaatc acttcaaata tcagacccgt gagcactaat atggtcgtca
     2041 ttgaaagaac cgaaactgag cgggcccccc aacggtgctt aagagaggtt agggtcattc
     2101 ttagtact
//
LOCUS       DAOMXO010000432         2106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30781_length_2106_cov_4.702584, whole genome shotgun sequence.
ACCESSION   DAOMXO010000432 DAOMXO010000000
VERSION     DAOMXO010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30781_length_2106_cov_4.702584"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLH57_08780"
     CDS             complement(<1..262)
                     /locus_tag="PLH57_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK02745.1"
                     /translation="MADDNQIEFFSSPEKLSPEASSLNSQRPLADRMRPRRLEEYFGQ
                     HHLAATNASLRRAIEKDLVGSMIFWGPPGTGKTTLARIVATHT"
     gene            370..>2106
                     /gene="ppdK"
                     /locus_tag="PLH57_08785"
     CDS             370..>2106
                     /gene="ppdK"
                     /locus_tag="PLH57_08785"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRK02746.1"
                     /translation="MATQYTYFFGNKSAEGDGSQKDLLGGKGANLHEMTKLGLPVPAG
                     FTISTEVCTHYYANGRKYPSELDGQVMASLKKIEETMGLRFGDPERPLLVSVRSGARA
                     SMPGMMDTILNLGMNDRVCEGIVGISKDERFAYDSYRRFITMYSDVVLNVNSNFFEDA
                     LDRKKESRGVHNDTDLTAADWKALVKEYKGIVLEQTGKKFPEDPIEQLWGAVGAVFGS
                     WMNPRANTYRKLNDIPESWGTAVNVQAMVFGNMGDDCATGVAFTRDPSTGERKFFGEF
                     LINAQGEDVVAGIRTPQPINDSSKNDANKSLVTLERAMPKAYAELVTVYQLLEKHYRD
                     MQDIEFTIQQNKLYMLQCRSGKRTAAAALRIAVEMEKEGLIDKKEAVMRVKPEQLDQL
                     LHPTLDPKAKKEHLCKGLPASPGAATGQVVFDPNTAEKWVNELGRKIILVRVETSPED
                     IHGMSVAQGILTARGGMTSHAAVVARGMGKCCVAGCGALKISYERKEMTIDNRVIKEG
                     DFITLDGSAGEVMLGKVETIAPAMNEHFKILMNWVDDFRKLKIRTNADTPLDSKVARS
                     FGAEGIGLCRTEH"
BASE COUNT          576 a          461 c          572 g          497 t
ORIGIN      
        1 cggtgtgggt ggcaacgata cgcgcgagag tggtttttcc ggttcccggt gggccccaga
       61 aaatcataga tcctaccaga tctttttcaa tggcgcgtcg cagcgaagcg ttggtcgcgg
      121 ccaaatgatg ttgtccgaaa tattcttcaa gacgtcttgg tcgcatgcga tcggcaaggg
      181 gacgctgaga attcagcgaa gaagcttcag gagaaagctt ttcgggagag ctaaaaaatt
      241 ctatttggtt gtcgtctgcc atttgtcgct cgctatcgtt cagtgccgtt tatcatagtc
      301 agcggtggaa agctcttgcc taaaacctgt tggccttctt atagttcgcc atgcagacgg
      361 aggttaggca tggcaacaca atatacgtat ttcttcggta acaagtccgc tgagggggat
      421 ggttcccaaa aagatctgct cggtggcaag ggtgcgaatt tacacgagat gaccaaactc
      481 ggattgcctg tacccgccgg ttttacaatt tccaccgaag tttgcacaca ctattatgcc
      541 aacggccgga agtacccgtc agaactggac ggccaggtaa tggcttctct taaaaaaata
      601 gaagaaacaa tgggactgcg tttcggagac cccgagcgcc cgcttctagt gtccgtgcgc
      661 tctggtgcgc gagctagtat gccaggtatg atggatacca ttttgaatct tggcatgaac
      721 gatcgcgtgt gcgaaggaat cgtcggaatt tcgaaagatg agagattcgc ctacgatagt
      781 tatcgaagat ttattacgat gtattcggat gtcgttttaa acgtgaattc gaattttttc
      841 gaagatgccc ttgatcgaaa aaaagaatca agaggcgttc acaatgacac cgatcttacg
      901 gctgcggatt ggaaagcgct ggttaaagag tacaaaggta tcgttttaga acaaaccggc
      961 aagaagtttc cagaagaccc gattgagcag ctttggggag cggtcggggc agtatttggt
     1021 tcttggatga accctcgcgc aaacacctat cgaaaactaa atgacattcc tgaatcttgg
     1081 ggaacggcgg taaatgttca ggcgatggtg ttcggaaata tgggcgacga ttgcgcgact
     1141 ggagtggcgt ttactcgcga cccttccacc ggtgaaagaa aattttttgg agaattccta
     1201 atcaacgctc aaggcgaaga tgtcgtggct ggcattcgta ccccacaacc gattaacgat
     1261 tcttctaaga acgacgcgaa caagagtttg gttacactcg aaagagcgat gcccaaggct
     1321 tacgctgagc tcgtgacagt gtaccaactg cttgaaaaac actaccgaga catgcaggac
     1381 atcgagttta ccattcaaca aaacaagctt tacatgttgc agtgccgctc cggaaagcgc
     1441 acggctgcag ctgcgcttcg aatcgcagtg gaaatggaaa aagagggttt gatcgataag
     1501 aaagaagcgg tcatgcgtgt gaaaccggag cagctcgatc aattgctgca tccgacgttg
     1561 gatccgaagg caaaaaaaga acatttgtgt aaaggcctac ccgcaagtcc gggagctgca
     1621 actggtcagg ttgtgtttga cccgaacaca gctgagaagt gggtcaacga actcggtcga
     1681 aaaataatct tggtaagagt tgaaaccagc ccggaagaca ttcacggcat gagtgtagct
     1741 caaggtattt taaccgctcg cgggggaatg acttctcacg cagcggtcgt tgctcgaggg
     1801 atggggaagt gctgcgttgc cggttgcggt gctcttaaga tttcgtacga acgaaaagaa
     1861 atgacgatcg ataaccgagt catcaaagaa ggcgacttca ttactctcga cggaagtgcc
     1921 ggagaagtaa tgttgggcaa ggtcgaaacc attgcaccag ctatgaacga gcattttaaa
     1981 attcttatga actgggtaga cgattttcga aagctcaaaa ttcgaaccaa cgccgatacc
     2041 ccactggact ctaaggtagc tcgtagtttc ggagctgaag gcatcggact gtgtagaact
     2101 gagcac
//
LOCUS       DAOMXO010000433         2102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30860_length_2102_cov_4.547142, whole genome shotgun sequence.
ACCESSION   DAOMXO010000433 DAOMXO010000000
VERSION     DAOMXO010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30860_length_2102_cov_4.547142"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PLH57_08790"
     CDS             complement(<1..530)
                     /locus_tag="PLH57_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011610342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRK02747.1"
                     /translation="MRSELENKRIVVTGGSGFLGRHIVSELENQGAESVFVPRSKEYD
                     LRDMSQCMRVCERADIVIHLAAHVGGIGLNQQKPGELFYDNAIMGIQLIEAARRQKVA
                     KLVVAGTICAYPKFTPVPFKEENLWEGYPEETNAPYGVAKKALLVQLQSYRAQYGLNG
                     IFLLPVNLYGPHDNFDL"
     gene            complement(542..1558)
                     /gene="gmd"
                     /locus_tag="PLH57_08795"
     CDS             complement(542..1558)
                     /gene="gmd"
                     /locus_tag="PLH57_08795"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRK02748.1"
                     /translation="MDQKIALITGITGQDGSYLAELLLEKGYTVHGIIRRSSSFNTDR
                     IDHLYRDPHLSGVRLFLHYGDLSDSTGINRILRKIKPDEIYHLGAQSHVRVSFDTPEY
                     TADVTGVGTIRLLDAILDTGINTKFYNAGSSEMYGKVQEIPQKEDTPFYPRSPYGCAK
                     VFSYWSTVNYREAYNLFACNGILFNHESPRRGETFVTRKITRALAQILAKKQDKLYLG
                     NLDAKRDWGFAKDYVEAIWLMMQQDKADDYVIATGETHSVREFLDEAFGYVGLDWKKY
                     VEIDQRYFRPTEVDLLVGDPAKAKKHLGWKPTLSFRELARLMVREDLKNAGIDPEQHI
                     TNRG"
     gene            complement(1578..>2102)
                     /locus_tag="PLH57_08800"
     CDS             complement(1578..>2102)
                     /locus_tag="PLH57_08800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK02749.1"
                     /translation="DVFFGSRLSPEKGIKTFLTAIENLPQLKFAMAGDGELKGAVLDA
                     AQRLPNLTYLGVLSRSDVLARVKKCKITVLPSQCNEALPTSVIEAFVCGKRCVIPDME
                     STRWLADKPFPGVLANTFDAVQLASAILKAAEMSPIPTEQINEMKAFFGFDRYRINLG
                     NLIEKTLAKPSGLR"
BASE COUNT          540 a          508 c          489 g          565 t
ORIGIN      
        1 agatcaaagt tatcgtgtgg accgtagaga ttcacgggta acaaaaaaat tccgttgagt
       61 ccgtattggg cgcgatagga ttgaagctga acaagtagag cttttttggc gacaccatag
      121 ggcgcatttg tttcttctgg ataaccttcc caaagattct cttccttaaa cggcaccgga
      181 gtaaatttcg gataggcaca aatagtacct gcgactacga gcttcgcgac tttttggcgc
      241 cgcgcagctt ctataagctg aatgcccatg attgcgttgt cataaaataa ttcgccaggt
      301 ttttgctgat tgagtccaat gccacctacg tgggccgcta gatgaataac gatatccgcg
      361 cgctcgcaca ctctcatgca ttgtgacatg tcgcgaaggt cgtattcctt cgatctaggt
      421 acaaaaacgc tttcggctcc ctgattttcg agttcgctca ctatatggcg accaagaaaa
      481 ccggatccac ctgtcactac tatgcgttta ttctctaatt ccgatcgcat gaaaatattg
      541 tttacccgcg attggtgatg tgttgctcgg ggtcaatgcc tgcatttttt aaatcttctc
      601 tgaccataag acgcgccaat tctctgaatg aaagagtcgg tttccagcca aggtgttttt
      661 tggcctttgc cggatcacca acgagaaggt cgacttcggt ggggcgaaaa taacgttggt
      721 cgatttccac gtattttttc caatctaacc ctacatagcc aaacgcttcg tcaagaaact
      781 cacgcaccga atgcgtctcg cctgtcgcga tcacatagtc gtcagcttta tcttgctgca
      841 tcataagcca aatcgcttcg acatagtctt ttgcgaaccc ccagtcacgt ttcgcatcga
      901 gatttccaag ataaagcttg tcctgtttct tagctaaaat ttgggccaat gcgcgggtga
      961 ttttgcgagt tacgaaggtt tcaccacgac gaggtgattc gtgattaaac aatatgccgt
     1021 tgcaggcgaa aagattgtag gcctctcggt aatttacggt agaccagtac gaaaaaacct
     1081 ttgcgcatcc ataggggcta cgcgggtaaa aaggcgtgtc ttctttttgg ggaatttcct
     1141 gcaccttacc gtacatttcg cttgaaccag cgttgtagaa cttggtgttg attccggtat
     1201 ccaaaatcgc gtcgagcaaa cgaatcgttc caacgcccgt gacgtcggcc gtgtactcgg
     1261 gggtgtcaaa acttacccgt acatgggact gagctccgag gtggtaaatt tcatcgggtt
     1321 taatttttcg aagaatgcgg ttgataccgg tggaatctga aagatcgccg tagtgcaaaa
     1381 acaaacgcac acccgacaaa tgtgggtccc tgtagagatg atcgattcga tcggtattaa
     1441 acgaactcga tcggcgaata atgccatgga cagtgtaacc tttttcaagc aagagctcgg
     1501 cgaggtaaga accgtcttgt cccgtaattc ccgtaataag cgctattttc tgatccattt
     1561 ttgccatagg gcccaagcta acgaaggcct gaaggcttcg caagagtctt ttcgataagg
     1621 ttccctaggt ttattcggta ccgatcaaac ccaaaaaacg ccttcatttc gttgatttgt
     1681 tcggttggaa taggcgacat ttccgcggct ttaagaatcg ccgacgccaa ctggaccgcg
     1741 tcgaaggtgt tggccaacac ccccggaaag ggtttatctg ccaaccaacg cgtcgattcc
     1801 atgtcgggaa tgacacaacg ttttccacaa acaaaagcct cgattaccga cgttggcaaa
     1861 gcttcattac attgacttgg cagtaccgtt attttgcatt ttttgactcg ggccaacaca
     1921 tctgaccgcg acaagacgcc taggtaggtg agattgggca gacgttgagc ggcatcaagt
     1981 accgcacctt ttaattcgcc gtcacctgcc atcgcgaact tcagctgcgg aagattttcg
     2041 atagcagtca aaaaagtctt aatacctttt tcaggagaaa gacggcttcc aaaaaaaaca
     2101 tc
//
LOCUS       DAOMXO010000434         2099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30918_length_2099_cov_5.040117, whole genome shotgun sequence.
ACCESSION   DAOMXO010000434 DAOMXO010000000
VERSION     DAOMXO010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30918_length_2099_cov_5.040117"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLH57_08805"
     CDS             <1..499
                     /locus_tag="PLH57_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRK02750.1"
                     /translation="ACQFGVDLCLEFGDRGNRISLLNGSGTVFDIQVGTRNNPNVDRI
                     TFFDSSSADVGVVALGINLSNVADKASAQNTLTSIDNAIVSVSSIRADFGAMQNRLQS
                     IINNLAISIENMSAANSRIRDTDVALETAELTKNNILVQAGTAVLSQANASIQGALSL
                     LKGTG"
     gene            865..1698
                     /locus_tag="PLH57_08810"
     CDS             865..1698
                     /locus_tag="PLH57_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRK02751.1"
                     /translation="MGFRIVTNTSAINAQRTLGNNRRMLDKSLEKLASGSRINRSGDD
                     AAGLAISENLIAQIRGLRQAKRNAQDGISLIQVSEGGLNEISSILIRLRELAVQAASD
                     TIGDTERKFTDREFQNLKDEVDRISNATEFNGTPLLNGRAGILEFQVGTRNNPILDRI
                     VYNGEGANATLAALKLGGESVSTKLGAQTSLAVIDDSLIQVNSIRADLGAMQNRLQST
                     INNLAIADENLAAANSRIRDVDVAEESAELTRNSILLQAGTSVLAQANMASKLALKLL
                     E"
     gene            1891..>2099
                     /locus_tag="PLH57_08815"
     CDS             1891..>2099
                     /locus_tag="PLH57_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02752.1"
                     /translation="MFARLTIVLLLTAMSAHAQMMGVGGMFYPFKHSSEMTCEEAINS
                     LNGVSAANLSGLSDFQAPFVMKDGK"
BASE COUNT          549 a          516 c          552 g          482 t
ORIGIN      
        1 cgcttgccag ttcggcgtcg acctgtgcct cgagttcggc gaccgaggaa accgcatctc
       61 tctacttaat ggtagcggta cggtgttcga tattcaggtc ggtacccgca ataatccgaa
      121 cgtcgaccgt attacgtttt tcgattcatc cagtgccgac gtcggtgtgg ttgctctagg
      181 aattaacctt tcaaacgtgg ccgacaaagc tagtgcgcag aatacattga cgtcaatcga
      241 caacgcgatc gtgtcggttt cgtcaattcg cgccgacttc ggtgccatgc agaatagatt
      301 gcagtccatt attaacaacc tggcgatcag catcgagaac atgagtgcag ccaacagccg
      361 aattcgcgac accgacgtgg cactcgaaac agccgagctc acgaagaaca acattctggt
      421 tcaagcgggc acggcggtac tctctcaagc taacgcgtca attcaaggcg cgctgagctt
      481 gttgaaaggg actggttaaa gacttaaacc cgctgtctca cttgctagac gctttcgcgg
      541 cgcctggcta gagagcagag gttaagacag gcaattgcct ggtctgtttc ctgtggactt
      601 tatgtgtggg tgatctcacg gagtgggatt acccgcacat cagtcacctt tgtgagtcaa
      661 ggcacgaggg ggcagatgta agtaaagtcc aaaaaatggc gggagaataa aatcttacgc
      721 cagggttttt gggaaacggc ctgctttgcg cgagcgaagc gggtaacgca gtacttgttt
      781 caaagcgacc aaggaacggt ccgccctcgt atggatacgc gggatatttc aaggaggatg
      841 ggtttatgaa cggaggctag aaacatggga tttagaatag taaccaatac ctcggcgatc
      901 aacgctcaac gtactttagg caacaaccga cggatgctag acaagagttt ggaaaaactc
      961 gcgtctggta gccgaatcaa ccgatcgggc gacgacgctg ctggtttagc gatctctgaa
     1021 aacttaatag ctcagatccg aggcttaagg caggcaaagc gaaacgcgca agacggcatc
     1081 agtttgattc aggtgtcaga aggcggcttg aacgaaattt caagcatcct cattcgtcta
     1141 cgcgaacttg cggtgcaagc tgcttcagat acgattggcg atacggaacg taagttcacg
     1201 gatcgcgagt ttcagaatct gaaggacgaa gtcgatcgta tttccaacgc cacggaattc
     1261 aacggtactc cgctgcttaa cggccgcgcc ggtatcttgg agttccaagt tggcacacgc
     1321 aacaatccaa tcctggatcg tatcgtgtac aacggtgaag gtgcaaacgc cacgcttgca
     1381 gcattgaagc ttggcggcga atcggtttcg acgaaactcg gagcgcaaac gtccctcgca
     1441 gttattgacg actcgctgat tcaagtgaac tccatacgcg ccgacctagg tgcgatgcag
     1501 aatcgcttgc aaagcacgat caacaacttg gcgattgcag acgaaaactt ggcagcagcg
     1561 aattcacgta tccgtgacgt cgatgtagcc gaggaaagcg ctgagctgac tcgtaacagt
     1621 atcttgctcc aggccggtac ttcggtactt gcccaagcca acatggcgag caagcttgca
     1681 ttgaagttgt tagaatagtc caaaaaccct ggcagccccg gctggcgcgc gacccttcgc
     1741 ggctagtcgg ggttccttat tttggcaatc caaacggttt ttgcgaccga ttttgaacta
     1801 aagtaaaagc taacctacaa aatttgccga gttgtttaaa gcgtgccttt tgcgaaaatc
     1861 agagatgaaa actttcgcca aggagccaaa atgtttgctc gtctcacaat agttcttcta
     1921 ttaactgcaa tgtccgccca cgcccaaatg atgggagtgg gaggaatgtt ctaccctttt
     1981 aaacattcct ctgaaatgac gtgcgaagaa gccattaact cgctcaatgg tgttagcgct
     2041 gcaaaccttt cgggattaag cgactttcag gccccgttcg tcatgaaaga cggtaaaat
//
LOCUS       DAOMXO010000435         2096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30978_length_2096_cov_5.176874, whole genome shotgun sequence.
ACCESSION   DAOMXO010000435 DAOMXO010000000
VERSION     DAOMXO010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30978_length_2096_cov_5.176874"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /gene="pilM"
                     /locus_tag="PLH57_08820"
     CDS             complement(<1..927)
                     /gene="pilM"
                     /locus_tag="PLH57_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRK02753.1"
                     /translation="MTEVIIKVMGLFGSSTQIGVSIGGSSIKVAELKKSGKSFALQRF
                     GIAQLPEDAIVNREIINHMGVVDSVRSLVDQLKIKGRSVVTSVSGAGVIVKKILLDQV
                     PQKELADAVLWEAEQYVPFNMNEVVHDFQVLNKSGPEGKMEIMLVACKRATVESFEAV
                     LKDAGLNTQCIDVDFFALENIFEANYPVDTASAIVDVGASSLKLVVCHNGQPLFTRDS
                     AIGGKTLTMEIQKHLNLSYQEAELLKIDGNSNGQMPQEVSDLMHVMAENFAAEIKRSM
                     DFFTASNSAVHVSYILLAGGSARLPNLSKIVED"
     gene            complement(1012..1512)
                     /locus_tag="PLH57_08825"
     CDS             complement(1012..1512)
                     /locus_tag="PLH57_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02754.1"
                     /translation="MICVGLFIYPEARDYQYRWSALRGARKIALEINRLRTQAIIGRK
                     ALEMRFIGPAQITIHEVENCGPQAQGKQIAEMQLESFDPNLVFAPTEWVRNEVIPILD
                     DPELTAERVANRYCYDALYGSSLQADGVASTSIYVLHQDSLKKQRPYDTVGIRLSGTN
                     GDVQFF"
     gene            complement(1598..>2096)
                     /locus_tag="PLH57_08830"
     CDS             complement(1598..>2096)
                     /locus_tag="PLH57_08830"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HRK02755.1"
                     /translation="YLHFGFSVLTVYYAFFAAALIAVTFIDLDFRIIPDWISILGMVL
                     GLIGAMIHPALSASSHVLGLLFGGGVFWLFSFLYLKWTGRDGLGFGDVKLLAMIGAFL
                     GLQGAIGTLLASSVIGSIVGLSLMFIQKKDLKMAIPFGPFLAIGALIMLFWGDSFVLR
                     FYSGE"
BASE COUNT          618 a          507 c          429 g          542 t
ORIGIN      
        1 gtcttcgaca atttttgata aatttggcag ccgcgcgctg ccaccagcga gcaaaatata
       61 ggacacgtga actgcgctgt ttgaggccgt gaaaaaatcc attgagcgct ttatttcggc
      121 ggcaaaattt tctgccatca cgtgcatgag atcggaaact tcttggggca tttgaccatt
      181 cgagttgccg tcgattttta gaagttcggc ttcctgataa cttaaattta aatgtttttg
      241 aatttccatc gtcaaggtct tgccaccgat ggcgctatca cgcgtgaaga gcggttgacc
      301 gttatgacag actaccagct ttaggctact cgcccctaca tcgacaatgg cagacgctgt
      361 atcaaccggg taattagctt caaatatatt ttccaaggca aaaaaatcga catcaatgca
      421 ctgagtattt aatccggcgt cttttagaac agcctcgaag ctttctactg ttgccctttt
      481 gcaggccacc aacatgattt ccatttttcc ttcgggacca cttttgttga gtacttgaaa
      541 atcatgcacg acttcgttca tgttaaatgg cacgtattgt tcggcttccc agagaactgc
      601 atcggcaagt tccttttgcg gaacttgatc gagaagaatt ttttttacaa tgactcctgc
      661 acccgaaaca gacgttacta ctgacctacc tttaatttta agctgatcga ctagactacg
      721 tacgctatcc acaacgccca tgtgattgat aatttcacga ttcacaatgg cgtcttctgg
      781 aagctgagca ataccaaaac gttgaagcgc aaaagatttt ccgcttttct tgagctccgc
      841 aaccttaatg ctgctgccac caatacttac accgatctga gtagacgaac cgaaaagtcc
      901 cataacttta atgataacct cagtcattgg agcgtcaaag caaaggtgca caataagagg
      961 ggaagaatct gcaggggtcg cgtcaagaat ttgacacaaa agaaaaacga actaaaaaaa
     1021 ttgaacgtcg ccgttagtcc cagaaagccg aatacctacc gtatcgtagg gtctttgttt
     1081 ttttaggctg tcctggtgca gaacgtaaat agacgtgctc gccactccat cagcctgaag
     1141 tgacgaccca tataacgcat catagcaata gcgatttgca actctttctg cagtcagttc
     1201 tggatcgtcg agtattggaa tcacttcgtt gcgtacccat tcagtggggg caaatactaa
     1261 gttagggtcg aaactttcca actgcatttc cgcgatttgt ttaccttgag cctgggggcc
     1321 acaattttct acctcgtgaa tcgtaatctg cgccggcccg ataaatcgca tttcaagtgc
     1381 cttacggcca atgatcgctt gggttcgcaa gcggttaatt tccaacgcta tttttcgagc
     1441 cccacgaagt gccgaccatc gatattgata gtcacgggcc tctgggtaaa taaaaagacc
     1501 aacacagatc acgaacgcga aaattgtaag cgccgttcct ttttctttcg cgctactgtt
     1561 tctgagtcaa tgatctcaat ttgagtcgcc attacattta ttctccggag taaaaccgca
     1621 gtacaaacga gtcaccccag aataacataa ttagggcacc aatagctaaa aacggaccaa
     1681 aaggaattgc catcttaaga tctttttttt gaatgaacat taggcttaat cccactattg
     1741 aaccaatcac cgaacttgcg agcagagtcc caatggcacc ttgaaggcca agaaacgcac
     1801 cgatcatggc aagcaacttt acgtcgccaa aacctaatcc gtcacgcccc gtccatttta
     1861 gatacaaaaa ggaaaacaac caaaacacgc ctccaccgaa tagaagtcct aaaacgtggg
     1921 aagatgcgct taaggcaggg tgaatcatgg cgccgattaa tcccaacacc atgcctaaaa
     1981 ttgaaatcca atctggaatt attcggaaat cgaggtcaat aaaggtaacc gcaatcaaag
     2041 ccgctgcaaa aaaggcgtaa taaactgtca gaactgaaaa accgaaatgc aaatag
//
LOCUS       DAOMXO010000436         2096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_30979_length_2096_cov_4.868202, whole genome shotgun sequence.
ACCESSION   DAOMXO010000436 DAOMXO010000000
VERSION     DAOMXO010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30979_length_2096_cov_4.868202"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..846)
                     /gene="lexA"
                     /locus_tag="PLH57_08835"
     CDS             complement(151..846)
                     /gene="lexA"
                     /locus_tag="PLH57_08835"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRK02756.1"
                     /translation="MSTEHFLTPKQQQLLAFIQGYQQAHGFAPTQQEIAKQFGFRSLG
                     TVQNYLVRLERQGLLKKSWNARRSLQPQPLSAAKSASLSGSLKKTLALSSHSFFSLPL
                     LGRVAAGLPIEAVESKEHVDIPAHMLRSAKGTLREVGDHFVLRVVGDSMIDDGILDGD
                     IVVLRKTPIAENGQTVVALIQNEATIKRFYKKAGRIELHAANAKYAPIIISEEDEKSF
                     SIAGVLSGVLRTY"
     gene            complement(879..>2096)
                     /locus_tag="PLH57_08840"
     CDS             complement(879..>2096)
                     /locus_tag="PLH57_08840"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRK02757.1"
                     /translation="GAIYSSAYVRLTGVTLSNNTAGGGGAFYAGYGSTAYFDRVLASE
                     NTALGGGTLMSAGYVSVLNSKFERNWATEGVGGAIAGTADLNVRNSVFLANSAKTKGG
                     AIYYRSRDFLNPLYRLSVVHSVFTNNESTNGSGGAVSMNPGSVHDSLFENNAAVAAYG
                     GALEGVSAIVNRSVFRNNSALSGGALQIYGTDVFSSAVYGNSASERGGAIHSSGGVRI
                     LNSTIDSNYAGVLGSAIAHELESLQIKNCTISDNGDGSSPAVWASVFASAWIGHTAIK
                     GAGPRCDFDGGIVSAGYNVEDGASCGLTTGSDRQNTTVALGALAGNDGPTVGSPYFSY
                     TATLPSRLPLGVRNALVDRGADFANQTDDLGSDGCLKLDVRGLARPEGRACDIGAVEL
                     EGLVSEPSPSSGT"
BASE COUNT          549 a          585 c          478 g          484 t
ORIGIN      
        1 gagcctggtt tgccagccaa ctcaaaaaca tgtcccgcgt aaacgggatc cgcactccat
       61 gtacaacgaa actcttttcg cttaaaagag ctctcggcaa tcaaggcctt tgcaaacatt
      121 tccttcaaaa ccgccgcatt cactttcact ttagtaggtc ctcagaacgc cacttaaaac
      181 cccggcgatt gaaaaacttt tttcatcttc ttcagaaata atgatgggcg cgtatttggc
      241 gttagccgca tgcaactcaa tacgacctgc ttttttgtaa aatcttttaa tagttgcttc
      301 gttttgaatt agggccacga cggtctgccc gttttcagcg atcggcgttt ttcgtaacac
      361 aacgatatct ccgtctaaga tgccatcgtc gatcatcgaa tcacctacaa ctcttaaaac
      421 gaagtgatca ccaacctcac gtaaagttcc cttggcactg cggagcatgt gcgcaggtat
      481 atcgacgtgt tctttagact ctactgcttc gataggaaga ccggccgcca ctcgccccaa
      541 aagcggtagt gaaaaaaaac tgtgggagga gagcgccagc gtctttttga gcgaaccgga
      601 gagcgaagca gacttcgccg cagagagtgg ttgtggttgc agacttcgac gcgcattcca
      661 ggattttttc aaaagaccct ggcgctccaa acggaccaag taattttgaa cagttccaag
      721 cgaacgaaag ccaaactgct tggcaatttc ttgttgagtc ggagcaaacc catgagcctg
      781 ttgatagcct tgaatgaagg ccaacaactg ctgctgtttt ggagttaaaa aatgttccgt
      841 actcaccctt aaaagggtat gcgtaagtta tgcgtaagtc aagtaccgct cgatggactt
      901 ggttcgctca ccaacccctc cagttcgacg gcaccaatgt cgcaggctct accctcgggg
      961 cgagcaagcc cacgaacgtc gagttttaaa caaccatcac tacctaaatc atcggtttga
     1021 ttcgcaaagt ctgcgccacg gtccaccaac gcgtttcgaa cccccagcgg aagacgggaa
     1081 ggcaatgtgg ccgtgtagga aaaatacggg cttcccaccg taggcccatc attaccggca
     1141 agggcaccta gtgccaccgt cgtattttgg cgatctgacc cagtcgttaa cccacagctc
     1201 gcgccatctt ctacgttgta accggccgaa actatccctc cgtcgaaatc acaacgaggc
     1261 ccggcgccct taatggccgt atgaccaatc caggcagacg cgaacaccga cgcccaaaca
     1321 gcgggtgaac tgccatcgcc gttgtcagaa atcgtgcaat tttttatctg aagagattca
     1381 agttcgtgag ctatcgcgct cccaagtacg ccggcgtagt tcgaatcaat agtggaatta
     1441 agaattctaa cgccacccga actatgaatc gctccaccac gttccgaagc tgaattgcca
     1501 tagacagcac tcgaaaatac gtcagttcca tagatttgca acgctccgcc actgagcgcg
     1561 gagttattgc gaaatactga tcggttcaca attgccgaaa cgccttcaag tgcgccgccg
     1621 taagcagcca ctgccgcgtt gttttcaaag agcgaatcgt gaacagagcc tgggttcatc
     1681 gaaactgccc cgcctgagcc gtttgtggat tcgttattcg taaacaccga atggacaaca
     1741 gacagtcgat aaagcgggtt caaaaagtcc ctcgaacgat aatagatcgc tccacccttt
     1801 gttttcgctg agtttgcaag aaagaccgaa ttgcgaacgt ttaaatccgc cgttcctgcg
     1861 atggcccccc ctactccctc ggtggcccaa tttctttcaa acttcgaatt caagacactg
     1921 acataaccgg cgctcattag agtaccaccc ccgagcgcgg tattttcaga ggctaagact
     1981 cggtcaaaat aagctgtgga cccatagccc gcgtaaaagg cgccaccgcc tccggccgtg
     2041 ttgttcgaga gcgtaacgcc cgttaaccga acataagcgc ttgaatagat ggcacc
//
LOCUS       DAOMXO010000437         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31017_length_2094_cov_4.842570, whole genome shotgun sequence.
ACCESSION   DAOMXO010000437 DAOMXO010000000
VERSION     DAOMXO010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31017_length_2094_cov_4.842570"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2065)
                     /locus_tag="PLH57_08845"
     CDS             complement(<1..2065)
                     /locus_tag="PLH57_08845"
                     /inference="COORDINATES: protein motif:HMM:NF016201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HRK02758.1"
                     /translation="MKIARQAKGAAGVLEFVTPGCPTCRSFYRDTSSFPVQISIMFHV
                     AGHNDFSVYSPFALRRQADPIAASLELAEMVQDYSRRFDADAVHLFYQYLSSFSFLQD
                     INYGEFTHPDHLNNAGLRDADLKRVVHGEGKRDPYREAGVIGPFPKQPNISGLQAMVG
                     YLKGRGPEWKYKLASKYEEMVRVYGYVPVTKIMNEGWATFLQIFLTAHSRWNNSKSVL
                     EFANLLSGVAYPQLTNPYWMGLEAWLRVYKNFVKENPIGPGMTMLERDRRFVQHAHGM
                     IEWMTDADFLRHALDEQWVTDMNLFLSRPASEQEVDPTLPPDPMHPDYNEQNVVVSRD
                     PKRVINFITDLVANKKKHLPRITVKNMRSLDRNVFHFAHEVYENYPLERSSTAQTLYV
                     AAQVLGAPMALDTIDQRKVIKQMPYPEDWPDFWRRFMPPPTTVTYEPVPIRVEVDLGG
                     KVAVFEQVKNAADGELLERENKEIATEMQAAIAAYQDSVTGLVNGDLERDWRHLYADS
                     GKMMMRIADHNTTHVIEGVVHDPNSSAAIAEYTRVIKSVGPARLRELMKRARRTATGI
                     AIPILPDIPEFSFDREMVETLKNLEPPAPVDANERIRPFEKILSATSKTSNSGVGALI
                     ASTTIDLSRYAGTSASVQMLLDQVKSFVNQAGRREIIFTGSDKAERLVQEVMNVLLKE
                     SVSTID"
BASE COUNT          530 a          532 c          487 g          545 t
ORIGIN      
        1 catcaatagt ggaaaccgat tctttcaata aaacgttcat tacttcttga accaaacgtt
       61 ccgccttatc ggagcccgta aaaataattt cacgtcggcc cgcttgattt acaaaactct
      121 tcacttgatc aagcagcatt tgcactgaag cggaagttcc ggcgtaacga cttaggtcga
      181 tggtggtcga tgcaatgagc gcgccgacgc cactgttaga ggttttgcta gtagcgctta
      241 aaattttctc aaatgggcgg atacgctcgt ttgcatcgac aggtgccgga ggctcgagat
      301 tttttagcgt ctctaccatt tcgcgatcaa aagagaattc aggaatgtcc ggcaaaattg
      361 gaattgcgat gccagttgca gtacgacgcg cgcgtttcat gagttcccga agtcgagcag
      421 ggcccaccga cttgatcacc ctagtgtact ccgcaattgc cgcggaggaa ttggggtcgt
      481 gcactacacc ttcgataaca tgagtggtgt tgtgatccgc gatgcgcatc atcattttgc
      541 cactatcggc ataaagatgg cgccaatctc tctcaagatc accatttacc agtcctgtta
      601 cactgtcttg ataagccgcg atcgctgctt gcatctcagt cgcaatttct ttgttttcgc
      661 gctctaaaag ttcaccatcc gcagcattct taacttgctc gaaaacggcg actttcccac
      721 caagatccac ttccactcga atcggaacgg gttcgtatgt caccgttgtt ggcggtggca
      781 taaagcgtcg ccaaaagtcc ggccagtctt ctgggtacgg catttgtttt attacttttc
      841 tttgatcaat cgtatcgagt gccatcggtg caccgagcac ttgagcagcc acgtagagtg
      901 tctgcgcagt actgcttctt tcgagaggat aattttcgta aacctcatgc gcaaaatgaa
      961 atacgtttcg atcgagcgag cgcatatttt ttaccgtaat gcgtggcaag tgctttttct
     1021 tatttgcgac caagtcggta ataaaattaa taactcgctt cggatctcgc gacactacta
     1081 cgttttgctc attgtaatcg gggtgcattg gatcaggcgg tagcgtagga tccacttctt
     1141 gttcacttgc tggccgggac agaaaaagat tcatatccgt gacccactgc tcatcgagcg
     1201 cgtgtcgaag aaaatcagcg tccgtcatcc attcgatcat gccgtgcgcg tgctgcacga
     1261 atcggcggtc gcgctcgagc attgtcatgc cggggccaat tggattttcc tttacgaagt
     1321 ttttgtaaac tcgaagccac gcctccaacc ccatccaata gggattcgtc agttgcggat
     1381 aagccactcc tgacaataaa tttgcgaatt ccagaacgct ctttgaatta ttccatcttg
     1441 aatgggctgt taaaaagatt tgcagaaagg tggcccaacc ttcgttcatg attttagtga
     1501 cgggaacata gccgtacacg cgaaccattt cttcgtactt cgaagcaagc ttgtacttcc
     1561 attccggccc gcggcccttt aaataaccga ccattgcttg aaggccgcta atattgggct
     1621 gcttgggaaa cggtccaatc actcctgctt cgcggtaggg atcacgctta ccctcaccgt
     1681 gcaccactcg cttaaggtcc gcgtcacgca atcctgcatt atttagatga tcaggatggg
     1741 taaactctcc gtagttgatg tcctgcaaaa aactaaaact gcttaagtat tggtaaaaaa
     1801 gatgcaccgc atcggcatcg aaacgccggg aatagtcttg aaccatttcg gcaagttcaa
     1861 gagaagccgc gatcggatca gcttgcctgc gcaacgcaaa aggcgaataa accgaaaaat
     1921 cattgtgccc agccacgtga aacatgatcg aaatttgaac aggaaacgaa gacgtgtcgc
     1981 gataaaaaga acgacaagta ggacaaccgg gcgttacaaa ctccaacaca ccagccgcac
     2041 cttttgcttg acgagcgatt ttcatgccgt cgtaccaatg cggaatcgcg tggt
//
LOCUS       DAOMXO010000438         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31109_length_2090_cov_5.852088, whole genome shotgun sequence.
ACCESSION   DAOMXO010000438 DAOMXO010000000
VERSION     DAOMXO010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31109_length_2090_cov_5.852088"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLH57_08850"
     CDS             complement(<1..696)
                     /locus_tag="PLH57_08850"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRK02759.1"
                     /translation="MTNSTVKDAAITKLIALESERQRDGLELIASENYVSPMVQEAQG
                     SILTNKYAEGYPGKRYYGGCEVVDQIEQIAIDRVKQLFQARAANVQPHSGSQANMAVY
                     FSCLKHGDKILGMNLSHGGHLTHGSPVNFSGRLFQTVFYGVEKESGRINYDEVRSIAK
                     KERPAAIVAGASAYARALDFKLFREIADEIGAKLIVDMAHIAGLVATGLHQSPIPHAH
                     FVTSTTHKTLRGPR"
     gene            complement(703..1137)
                     /gene="rpiB"
                     /locus_tag="PLH57_08855"
     CDS             complement(703..1137)
                     /gene="rpiB"
                     /locus_tag="PLH57_08855"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRK02760.1"
                     /translation="MTTKQPTLAIASDHAGFELKEWLRTNFTSATWIDAGCSSVARCD
                     YPDFAEKACQLVLSGKASAAVLICGSGVGMSMAANRKNGIRAVLAHSEIAARLSRAHN
                     DANVLCLGARITANEYALDILSQWLATPFEGGRHQDRIAKMG"
     gene            complement(1139..>2090)
                     /locus_tag="PLH57_08860"
     CDS             complement(1139..>2090)
                     /locus_tag="PLH57_08860"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRK02761.1"
                     /translation="VGVNVSAGMGGLPEIQEWDRELVSKEKKSITPFFVPMIIPNLIS
                     GHTSIFTNIQGPNLCVVTACATSAHALGEAKRIIERGDADIMVSGGAEAVVCEIGIVG
                     FASMRALSTRNDAPKEASRPYDKNRDGFVVAEGAAVLILEELEHAKKRGAKIYCELAG
                     YGYSGDANHITSPCADGSGAIRSMSGALKDAHISASQVNYVNTHGTSTTVGDVAEAQA
                     IAKLWGPDADALHISSTKSMTGHLLGATGSFEAAVCALAIQHSLVPPTINLNTLDDEC
                     AATKLNFTPNKAVEKKISAALTNSFGFGGTNASLVLREFK"
BASE COUNT          467 a          539 c          551 g          533 t
ORIGIN      
        1 acgcgggccc cgaagggttt tatgggtggt ggaagtcaca aagtgcgcgt gcggaatagg
       61 actttgatga agaccggtag cgaccaaccc tgcaatgtgg gccatatcga cgattaactt
      121 agctccgatt tcatcagcga tttcccgaaa aagtttaaaa tcaagtgctc gcgcataggc
      181 actcgctcca gctacgatcg cagctggccg ctcctttttt gcgatcgatc gcacttcgtc
      241 gtaattgata cgaccggatt ccttctcaac tccataaaac accgtttgga acaaacggcc
      301 cgaaaaattc actgggctgc cgtgagtgag atgacctccg tgggaaagat tcattccaag
      361 gatcttatcg ccatgtttga gacacgaaaa atacaccgcc atattggctt gggagccgct
      421 gtggggctga acgttggccg cgcgtgcttg gaagagctgt tttactcgat caatcgcgat
      481 ctgctctatt tggtccacga cttcgcaacc gccgtaatat ctttttccag ggtaaccttc
      541 ggcatattta ttcgttagaa tgctaccctg cgcttcttgc accatcggcg atacatagtt
      601 ctccgaagcg attagttcga gcccgtcacg ctgccgttca ctctcgagtg ctatcagttt
      661 agtgattgcg gcgtctttta cggtggaatt ggtcatgtgt atttacccca tttttgcgat
      721 acggtcctgg tggcggccac cttcgaacgg tgtggcaagc cactgactca aaatatctaa
      781 cgcgtattcg ttcgcagtga tgcgagcgcc gaggcaaaga acattggcgt cgttgtgtgc
      841 ccgcgaaaga cgggcagcga tctctgagtg agccaaaaca gcgcgaattc cgtttttgcg
      901 gttggcggcc atcgacattc caacaccgga cccacaaatc aatacggcgg cagatgcttt
      961 accactcaaa acgagttggc aggctttttc ggcgaagtcc ggatagtcgc agcgggcaac
     1021 tgaggagcag ccggcatcga tccaggttgc gctcgtgaaa ttggtacgga gccattcttt
     1081 taattcaaat cctgcatggt ctgaggcgat cgcgagagta ggctgtttcg tcgtcatttt
     1141 atttgaactc gcggagcacg aggcttgcgt tggttcctcc gaagccaaaa gaattcgtta
     1201 gagcagcgga gatctttttt tctaccgcct tattaggtgt gaagttaagt tttgtggctg
     1261 cgcattcgtc gtcgagcgta ttgaggttga tcgtcggagg taccaaacta tgctgaatcg
     1321 ctagtgcgca aaccgcggct tcaaatgagc ccgtggcgcc aagtaaatga ccggtcatgc
     1381 ttttggttga actaatatgg agtgcatcgg catcggggcc ccagagttta gcaatcgctt
     1441 gcgcttcggc aacgtcgccc accgtcgtcg aagtgccgtg ggtgttaacg taattaactt
     1501 gagaggccga gatgtgagcg tccttcaagg cgccactcat cgaacgaatc gctccgctgc
     1561 catcagcgca cggcgaagtg atgtggttgg catcgccgga gtaaccgtaa cccgcgagct
     1621 cacagtaaat cttagcgcct cgtttttttg catgttcgag ttcttccaag attagaacgg
     1681 ccgctccctc tgcaacaacg aaaccatctc gatttttgtc gtacgggcgc gaggcctctt
     1741 tgggagcgtc gtttctggta gataacgcgc gcatggaggc aaaacctaca atgccgattt
     1801 cacaaaccac tgcttcggcg ccaccagata ccatgatgtc cgcgtcgcca cgttcgatga
     1861 tccttttggc ttcgcccaat gcatgtgcgg acgtcgcgca ggctgttacg acgcatagat
     1921 tcggaccttg gatgttggta aatatgctcg tgtgacccga tatcaaattt ggaatgatca
     1981 tcggcacaaa gaaaggagtt atcgattttt tttctttcga aactagttct ctgtcccact
     2041 cctgaatttc ggggagaccg cccatgccgg ccgaaacgtt cactcccacg
//
LOCUS       DAOMXO010000439         2088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31162_length_2088_cov_4.131825, whole genome shotgun sequence.
ACCESSION   DAOMXO010000439 DAOMXO010000000
VERSION     DAOMXO010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31162_length_2088_cov_4.131825"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1066
                     /gene="htpX"
                     /locus_tag="PLH57_08865"
     CDS             155..1066
                     /gene="htpX"
                     /locus_tag="PLH57_08865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRK02762.1"
                     /translation="MAWFKRIGLFLAVNALVIITISTLMSVLGVKPYITAYGLNYESL
                     MIFCLIWGMGGAFISLGLSRVMAKWMMGVRVISPDTRDSELAGLVQMVHEMSRAAHLR
                     VMPEVGIYESPEVNAFATGPTKNRSLVAVSTGLLHRMRQEEVRGVIGHEVAHIANGDM
                     VTMTLVQGVVNAFVMFLARAIAYGLTMARGSDGEERQGTPMSYYLVQFALEMAFMVLG
                     SIVVAWFSRLREYRADAGGARLAGRDSMIRALEALRRTFEGVDPNAQPAIQALKISSR
                     PGGIMKYFSTHPPLEERIERLKRATAA"
     gene            1063..1572
                     /locus_tag="PLH57_08870"
     CDS             1063..1572
                     /locus_tag="PLH57_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HRK02763.1"
                     /translation="MKKIEHLFEGVIFSSRWVQAPIYGGLIVGAVLYSYKFLIELIHL
                     CTTVRTISEEMLMLGILTLVDISMVLNLLVMVIIGGYATFVSRMNLESHEDRPDWLQK
                     VDAGTLKVKLAGALVGISGIHLLKSFINIGNKDFTQVKWQIVIHVVFLFSTLMLAYTE
                     KILHSSHRK"
     gene            1569..>2088
                     /locus_tag="PLH57_08875"
     CDS             1569..>2088
                     /locus_tag="PLH57_08875"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02764.1"
                     /translation="MTNIISNVSVVPNWLWAAFSGGVLVLLLIDLSLFGRGDHKISRK
                     AALSESALWIAVALAFNAWFASKFGRELGLEFLTGYLVEKSLSVDNLFVILLILGSFR
                     VPEQYQHRVLFYGVLGAIVLRAIFILLGAHVLHKFHWILYVFGAVLIITAIKFLRETD
                     QKIDVKESLTIRL"
BASE COUNT          487 a          456 c          547 g          598 t
ORIGIN      
        1 ggcaatagcc cgaaagtaaa agagatgatg gtaattcaac cactgagcca cgggactcct
       61 ttattcgctt taaacgaagt atttaattat atttatcgta ttttaaaaag tatctgtcaa
      121 acctagaatg gacgttcgta ggaggatatt ttttatggct tggttcaaac gtatcggtct
      181 ttttctcgcg gtaaacgcgc ttgtgattat caccatttcg acactcatga gcgtcttagg
      241 ggtgaaacct tatattacgg cctacggctt gaactacgaa tcgctcatga tcttttgttt
      301 gatctggggc atgggcggtg cattcatttc gctcgggctt tcaagggtca tggccaagtg
      361 gatgatgggg gtccgggtta tttcgcccga cactcgcgat tccgagttgg ccggtttggt
      421 ccagatggtt cacgaaatgt cccgcgccgc gcacctacga gttatgcccg aggttggaat
      481 ttatgagtcg ccagaggtga atgcgtttgc gactggccct acaaagaata gatctctcgt
      541 cgcggtatca actggattac ttcaccgcat gcgtcaggaa gaagtgcgtg gcgtgattgg
      601 ccacgaagtg gctcacattg ccaacggtga catggtcacc atgacgttgg ttcaaggcgt
      661 agtgaacgcg tttgtgatgt ttctggcacg cgccattgcc tacggactta cgatggcacg
      721 tgggagcgat ggcgaagaac gccagggaac tccgatgtct tactatttgg tgcagttcgc
      781 actggaaatg gcatttatgg ttttgggttc catcgtcgtg gcctggtttt cgcgactacg
      841 cgaatatcga gccgacgctg gcggagcccg tcttgccggg cgtgacagca tgataagagc
      901 gcttgaagct ttgcgacgaa cgtttgaagg cgtcgatccg aatgctcagc cggctataca
      961 agctctcaaa atatccagtc gcccgggtgg gatcatgaag tatttttcta cccacccgcc
     1021 gctcgaagag cgcattgaaa gattgaagag agcgacagcg gcatgaaaaa aatcgaacac
     1081 ctcttcgaag gggttatttt ttcgagccgg tgggttcagg caccgatcta tggaggtctc
     1141 attgtcggcg cggtgctcta ttcctacaag tttttgattg agcttattca cctttgcacc
     1201 accgtacgaa ctatcagcga agaaatgttg atgttaggaa ttttaacgtt ggtcgacatt
     1261 tcaatggttc taaatctctt ggtgatggtc attatcggag gttatgcgac gttcgtgagt
     1321 cgaatgaacc tagagtctca cgaagaccga cctgattggc ttcaaaaggt ggacgccgga
     1381 acgctcaagg tgaaactcgc cggcgccttg gtgggtattt caggaattca tttactgaag
     1441 tcgtttatta atatcggaaa taaagacttt acacaggtga agtggcagat agtcatccac
     1501 gtcgtgtttt tgttttcgac tttaatgctg gcctatacag aaaaaatatt acactcctct
     1561 catcgcaaat gacgaatata atttcgaacg tatcagtggt tccgaattgg ctgtgggcag
     1621 cctttagtgg tggtgttctt gtacttctgc taattgacct tagtctcttt ggtcgtggtg
     1681 atcacaagat ttcccgcaag gcagccttgt cggaaagcgc actgtggatt gcggtggcgc
     1741 tggcctttaa tgcttggttt gcatcgaaat ttgggcggga actagggcta gaattcctca
     1801 ccggttattt ggttgaaaaa agtctaagtg tcgacaatct tttcgtcata cttcttatcc
     1861 ttggttcttt tcgagttccc gaacagtacc aacaccgcgt gctattttat ggagtgctcg
     1921 gggccatcgt attgcgggcc atatttattc tgcttggggc ccacgtgctc cataaatttc
     1981 attggatcct ttacgttttt ggggcggtgt tgattataac cgccataaaa tttctgcggg
     2041 aaaccgacca aaaaatcgac gttaaggaaa gtttaaccat ccggctac
//
LOCUS       DAOMXO010000440         2088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31164_length_2088_cov_3.808165, whole genome shotgun sequence.
ACCESSION   DAOMXO010000440 DAOMXO010000000
VERSION     DAOMXO010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31164_length_2088_cov_3.808165"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLH57_08880"
     CDS             complement(<1..283)
                     /locus_tag="PLH57_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRK02765.1"
                     /translation="MVILLLGPPGSGKGTQAKVLVERLGIPQLSTGDMLRAAVKDQTT
                     LGVKAQSFMKKGELVPDNLVIGLIEERVVQKDCQKGFILDGFPRNVAQAD"
     gene            complement(287..1585)
                     /gene="secY"
                     /locus_tag="PLH57_08885"
     CDS             complement(287..1585)
                     /gene="secY"
                     /locus_tag="PLH57_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRK02766.1"
                     /translation="MSGAQGLVRIPELRRRIIFTLMMLAVYRVGVHVSTPGVDTAALS
                     QFFENSKGTIFSWFNLFSGGALERFSVFALGVMPYISSSIIFQLLTVVWPYLHELQKE
                     GEQGRKKITQYTRYGTVLLCIIQGYGIAVGLEQMANPSVVIQPGWGFRLLTTITLTAG
                     TVFLMWLGEQITERGIGNGMSLIIFAGIAAGIPGGFSQTVSLVRTGEISFISLLMVGA
                     VIVATFALIIFVERGQRRIPVQYAKRIVGRKVYGGQNTHLPLKINSAGVIPPIFASSL
                     IMFPATIASFVSNQWTQKLQDLLQLGGMAYNVVYVTLIVFFAYFYTAVTFKSEDVAEN
                     LKKHGGFIPGIRPGQKTAEFLDHVLSRVTLGGAVYISLICVLPTILAEKAKVPFFFGG
                     TSVLILVGVALDTVAQIESHLLTRNYEGFVKSARIRGRRV"
     gene            complement(1634..2080)
                     /gene="rplO"
                     /locus_tag="PLH57_08890"
     CDS             complement(1634..2080)
                     /gene="rplO"
                     /locus_tag="PLH57_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006093108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRK02767.1"
                     /translation="MLKLGEIRNARGATSETRRRGRGSSSGRGGTAGKGHKGQLARAG
                     GRVRLGFEGGQMPLYRRLPKRGFTNHFSKEFAVINLADLNESEIKDVSIESLKESGLL
                     KTKLDRLKVLGTGTVERALTVKAHAISESAKKKIEKTGGTVQILGK"
BASE COUNT          595 a          541 c          441 g          511 t
ORIGIN      
        1 tgtctgcctg cgccacattc cgaggaaacc catcaagaat gaagcctttt tgacaatcct
       61 tttgaacaac gcgttcctcg attaacccaa tgacgagatt gtcgggaacc aactcccctt
      121 ttttcataaa ggactgggct ttgactccaa gggtcgtttg gtccttaaca gctgcccgca
      181 acatgtcacc ggtcgaaagc tgaggaatgc ccaggcgttc caccaatact tttgcctgtg
      241 ttcccttacc ggaccctgga gggcctaaca acaaaatcac catgaattat actcggcgac
      301 ctctgatacg tgcgctcttt acaaagcctt cgtagtttcg agtaagcagg tgcgattcaa
      361 tttgggcgac cgtatctaga gctacaccaa caagaatcaa aacgctggtt ccgccgaaga
      421 aaaatggcac cttcgctttt tcagcaagta tggtcgggag tacgcatatg agagatatgt
      481 atacggctcc gcctaatgtc actctggaaa gcacatgatc aagaaattct gcggtctttt
      541 gccccggacg aattccagga ataaatccac catgtttttt caaattctca gcgacgtcct
      601 cgctcttaaa ggtaacggca gtatagaaat aagcaaaaaa tactatcaga gtaacgtaaa
      661 cgacattgta cgccatgccg ccgagctgca ataagtcctg caatttctga gtccattgat
      721 tgctaacaaa actcgcaatg gttgccggaa acataataag agaagaagca aaaataggag
      781 gaataacgcc cgcactatta atcttgagag gcaaatgtgt attttgaccc ccatatactt
      841 ttcgaccgac aatacgtttt gcgtattgga caggaattcg ccgctgccca cgctcgacaa
      901 aaataattag ggcgaaagtc gccacaatga cggcgccaac cattaacagc gaaataaagg
      961 atatctcgcc agttctcacc aaacttaccg tttgactaaa gccacctgga attccagctg
     1021 cgatccccgc aaatataatg agggacatgc catttccaat accgcgttca gtgatttgct
     1081 cgccaagcca cattagaaaa acagtaccag ccgtgagagt aatggtggtc aataaacgaa
     1141 acccccagcc gggttgaata accaccgacg ggttcgccat ctgctctaaa ccaaccgcga
     1201 tgccgtaacc ttgaatgata caaagcaata cggtgccgta acgagtgtac tgagtaattt
     1261 tttttcgccc ttgctcgcct tctttttgaa gttcgtgcaa atagggccac acgaccgtta
     1321 atagctgaaa gataattgag gagctaatgt agggcattac gcccagagcg aataccgaaa
     1381 atcgctcaag cgcccctccg gagaaaaggt tgaaccaaga aaaaatggtc cccttggaat
     1441 tctcaaaaaa ctgagaaaga gcagcggtat ccacaccggg agttgagaca tgcacgccca
     1501 ctcgataaac agccaacatc attaaagtga agataatgcg tctacgaagt tcagggatcc
     1561 gaactaaacc ttgggcgcca gacattgttg cttattctcc cgcgtgtttc gaaataccaa
     1621 gttttgaagt taactacttg cccaaaatct gaacggttcc gcctgttttt tctatttttt
     1681 tcttagcgga ttcagaaatc gcatgggcct taactgttaa cgctctttct acagtccccg
     1741 tacccaaaac cttaagtcga tccagcttgg tcttaagaag tccgctttct ttcaaggatt
     1801 cgatagaaac gtctttaatt tcggactcat taaggtccgc caagttgata acagcgaatt
     1861 ccttcgaaaa gtgatttgtg aagccgcgct taggaaggcg acgataaagc ggcatttgac
     1921 caccctcgaa gcccaaacga acacgtccac ctgctctcgc gagctgtccc ttgtgacctt
     1981 taccggctgt tccacctcgt cccgaagaag aaccccgtcc acggcgccgt gtttcgctag
     2041 tcgcgcctct tgcattacga atttcgccta atttaagcat ttcctacc
//
LOCUS       DAOMXO010000441         2087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31193_length_2087_cov_4.889272, whole genome shotgun sequence.
ACCESSION   DAOMXO010000441 DAOMXO010000000
VERSION     DAOMXO010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31193_length_2087_cov_4.889272"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLH57_08895"
     CDS             complement(<1..840)
                     /locus_tag="PLH57_08895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRK02768.1"
                     /translation="MADSLRKFGKYFLLDLLAQGGMAEIYRARQAALDGGNRILAIKR
                     ILKSYSENSEFVAMFRSEIKTTSGFTHPNIVQVYDYGEEDGMLYIAMEWVDGKNLRQF
                     ISKFGELKTTFPLEVSCYLVEQAARALAYAHSYKDRFSGKSLNIVHRDISPQNILISY
                     DGAVKVIDFGIAKAVTNIEATRAGVIKGKPSYLSPEQIAGDSLDGRSDIFALGIVFWE
                     LLIGKKLFQGENDYAVLKLIENCGHTIKAPSLINPKIPKELDVIVMKCLAKNRSRGLR
                     SRWT"
     gene            complement(931..1641)
                     /locus_tag="PLH57_08900"
     CDS             complement(931..1641)
                     /locus_tag="PLH57_08900"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK02769.1"
                     /translation="MNSKTFLQENTLVPLAGQGSATLGELNDTQIFMCQEVGRGKRVV
                     DLGCGDGRMLERLNEQCNEAFGVEYNRRLVSQAQQRGLRVRNFDLNEGIPFEDEYFDC
                     VVASDVVDSIFDSRFMFEEVHRILRRRGIFLFSVPNLNSLSNRIQMIRGSYLKRAGGF
                     PEDTDGMRVRFFNTQKIYELCRFSGFHVHQIWGLPSAESSDAQGNRDVLRSIARPLYR
                     WRPEVADILVVRARRIDV"
     gene            complement(1649..>2087)
                     /locus_tag="PLH57_08905"
     CDS             complement(1649..>2087)
                     /locus_tag="PLH57_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK02770.1"
                     /translation="DKGMFVDPSGVYFHAEAQFVLSGFAIESEPDGFNFDYDGQSFFE
                     EHIWSALHERSSGFEMLKHVTGWAGLYEVSPDRCGIMGSVNNSSQIFEAHSFSGRGVM
                     QSWAVGRGLSELILNERFETIDLAALSGERFKEGKLVRENLLI"
BASE COUNT          561 a          547 c          421 g          558 t
ORIGIN      
        1 cgtccatcgc gatcgaagtc cgcgactgcg attcttagca aggcacttca tgacgatcac
       61 gtcgagctct tttgggattt ttggattgat caaactcgga gccttaatag tgtgcccaca
      121 attttcgatc aatttcagca ccgcgtagtc gttttcccct tgaaacaact tctttccgat
      181 taagagttcc caaaaaacaa tccccaatgc aaaaatatca cttcgaccat ctaacgaatc
      241 gcctgcgatt tgttcgggcg ataagtaact gggcttgcct ttgatcactc cggcacgagt
      301 cgcctctata ttggttaccg ccttggcgat tccgaaatcg attactttaa cggctccgtc
      361 gtaggaaatt agaatatttt gcggactaat atcgcgatgc acgatattta aactttttcc
      421 agaaaatcga tctttgtaag aatgcgcgta agcaagtgcc cgagccgctt gctcaaccag
      481 ataacaactc acttctagtg gaaaagtagt ttttagttca ccaaatttcg aaataaattg
      541 tcgaaggttt tttccgtcca cccattccat ggcaatgtat aacatgccgt cttcttcgcc
      601 gtagtcgtag acttgcacga tattgggatg agtgaacccg ctagtggttt taatttcact
      661 gcgaaacatg gcaacgaatt cagaattttc agaatatgat tttagaattc gtttgatagc
      721 aagaattcgg ttaccaccat cgagggctgc ttggcgtgca cgataaattt ccgccatgcc
      781 tccctgagct agtaaatcga gtaggaaata tttgccgaac ttccttaaac tatcggccac
      841 ccggaaccct ccgtctccag aagttatcgg aaggaggagg ttcaaatatt aggctcttgc
      901 gactaccaat tttgccggtt tcgggaacta tcagacgtcg attcgccggg cccgaaccac
      961 taagatatca gccacttcgg gccgccagcg gtaaaggggg cgagcgatgc ttcgcaacac
     1021 atcgcgattc ccctgcgcgt cggatgattc cgccgacggc aggccccata tctgatgaac
     1081 atgaaaccct gaaaacctac aaagttcgta aattttttga gtattaaaaa aacgaactcg
     1141 catgccgtct gtgtcttccg ggaaccctcc agctcttttc agataacttc cacgaatcat
     1201 ctgaatgcgg ttggaaaggg aatttaagtt cgggaccgaa aacaaaaaaa tgcctctacg
     1261 acgtagtatt cgatgcactt cctcgaacat gaatcgggaa tcgaatatgc tgtcaaccac
     1321 atcactggct actacgcagt cgaagtattc atcttcgaac gggatccctt cgtttaagtc
     1381 gaagttacgt acccgcaaac ctcgctgctg cgcctgcgaa actaagcgtc ggttgtattc
     1441 cacgccaaat gcttcattgc attgttcatt tagcctctcc agcattcggc catcgccaca
     1501 gcccaaatct acgactcttt ttcctcggcc gacttcttga cacataaaaa tctgagtgtc
     1561 attgagttcg ccgagcgtcg cacttccctg tcccgcaagc ggtactaagg tattttcttg
     1621 taaaaaggtc ttcgaattca tagtgagcct aaataagcag gttttctcga accagctttc
     1681 cttccttgaa acgttctcca ctcagagccg ccaagtcgat cgtttcaaat ctctcattta
     1741 aaatcaattc agatagtccg cgacctacag cccaagactg cattacacca cgccccgaaa
     1801 aagagtgcgc ctcaaaaatt tggctactat tatttaccga acccattatt ccgcatcgat
     1861 ccggggaaac ttcatagagt ccagcccaac cagttacgtg tttaagcatt tcaaacccac
     1921 ttgatcgctc gtgaagggct gaccagatat gttcttcaaa gaacgactgc ccatcatagt
     1981 cgaaattaaa accgtcaggt tcactttcga ttgcgaaccc gctcaatacg aactgcgctt
     2041 cagcatgaaa ataaactcca ctcggatcta caaacatccc tttgtcg
//
LOCUS       DAOMXO010000442         2083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31274_length_2083_cov_6.472880, whole genome shotgun sequence.
ACCESSION   DAOMXO010000442 DAOMXO010000000
VERSION     DAOMXO010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31274_length_2083_cov_6.472880"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /gene="groL"
                     /locus_tag="PLH57_08910"
     CDS             complement(<1..1635)
                     /gene="groL"
                     /locus_tag="PLH57_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRK02771.1"
                     /translation="MSAKELRFSEQARSSILQGVNVLADAVKVTLGPKGRNVVIEKAF
                     GAPSVTKDGVTVAKEIELENKFENMGAQMVKEVASKTNDDAGDGTTTATVLAQAIYRE
                     GAKIVAAGHNPMELKRGIDKAVEKIVEELKKISKPCKSEKEIAQVATISANNDSTIGK
                     IIAEAMEKVGKEGVITVEESKTAETTLDVVEGMQFDRGYLSPYFVTNPEKMEASLDSP
                     YILIYDKKISSMKDLLPLLEKVVQRSKPLVIVAEEVEGEALATLVVNKLRGTLHVCAV
                     KAPGFGDRRKEMLQDLAVLTGGKVISEDLGAKLENARLEDLGQAKKVSIDKDNTTIVD
                     GAGKKADVESRVKLIRKQIEETTSDYDREKLQERLAKLVGGVAVINVGAATEVEMKEK
                     KQRVEDALNATRAAVEEGIVAGGGTALLRAAAGLTKGKLDSAEQQAGYSIIRRAAEEP
                     LRMIAGNAGWEGSVVVDKVLSNDNPNWGFNALTETYEDLVKAGVIDPAKVTRCALQNA
                     ASVAALMLTTETLIADKPKKDSAGPAMPGGMGGMGGM"
     gene            complement(1660..1947)
                     /gene="groES"
                     /locus_tag="PLH57_08915"
     CDS             complement(1660..1947)
                     /gene="groES"
                     /locus_tag="PLH57_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRK02772.1"
                     /translation="MQVKPLHDRILVKRLSEEEKTKGGIIIPDTAKEKPAEGQVMAVG
                     AGRRTEDGKLISLEVKKGDKILFGKYSGTEIKIDGADYLIMREDDVLGIMN"
BASE COUNT          445 a          553 c          458 g          627 t
ORIGIN      
        1 catgccgccc attccaccca tgccacccgg catagcagga ccggccgaat ctttcttcgg
       61 cttgtctgca atgagagttt cagtggttaa catgagcgca gctacactgg ctgcgttctg
      121 tagagcgcaa cgagtaactt tcgcagggtc aatcacgccc gctttgacga gatcttcata
      181 ggtctctgtc aatgcgttga agccccagtt agggttgtcg ttcgaaagaa ctttatcaac
      241 cacaactgaa ccttcccaac ctgcattgcc agcgatcatg cgaagcggct cttcagcagc
      301 tcggcgaatg atcgaatagc cagcttgttg ttcagcgcta tcgagtttgc ccttggtcaa
      361 cccagctgcg gcacgaagca atgcggtacc accgccagca acgatgcctt cttcaactgc
      421 agcgcgagtt gcattcaaag cgtcttctac gcgttgtttc ttttctttca tttctacttc
      481 agtcgcggca cctacgttga ttacggctac gccaccaacg agttttgcaa gacgctcttg
      541 aagcttctca cgatcgtaat ccgaagtagt ttcttcgatt tgtttacgaa tgagtttcac
      601 gcgagactca acatcagctt tttttccagc gccgtcgacg atggtcgtgt tatctttgtc
      661 gatcgacact tttttagctt ggccgagatc ttcgagacgt gcattttcaa gcttcgcacc
      721 gagatcttcg ctgatcacct taccaccggt aagaacagcc aagtcttgga gcatttcttt
      781 acgacgatcg ccgaagcctg gcgccttcac tgcacaaacg tgcaacgtgc cacgaagttt
      841 gtttactaca agagtagcaa gcgcttcgcc ttcaacttct tcagctacaa taacgagcgg
      901 cttgctgcgt tgaacaactt tctccaacaa tgggagaagg tctttcatgc tgctgatctt
      961 cttatcgtaa ataagaatgt atgggctatc gagtgatgct tccatcttct ctgggttagt
     1021 tacaaagtac ggcgaaagat agccgcggtc gaattgcatg ccttctacta cgtcgagcgt
     1081 agtttcagct gttttgcttt cttcaaccgt gatcacgcct tctttgccaa ctttttccat
     1141 cgcttcagca atgatcttgc cgatcgtaga atcgttgttt gccgaaatcg tagcaacttg
     1201 cgcgatttct ttttccgact tgcaaggttt cgagatcttt ttgagttctt caacgatctt
     1261 ctctaccgct ttgtcgatgc cgcgtttcaa ttccattggg ttgtggcccg cagcgacgat
     1321 cttggcgcct tcgcgataaa tcgcttgagc caaaacggtc gcagtcgtcg tgccatcacc
     1381 agcgtcgtca ttggttttgg aagctacttc cttcaccatt tgcgcgccca tgttttcgaa
     1441 tttgttttcg agttcaattt ctttagccac ggtgacgccg tctttcgtta cgcttggagc
     1501 gccgaaagct ttttcaatca ccacgttgcg gccctttggt ccaagggtca ctttcactgc
     1561 gtccgcgagt acgttgacgc cttgaaggat cgatgaacgt gcctgttctg aaaaacgaag
     1621 ttcttttgca gacatttgtt gtcctcctag tttctaaatt tagttcatga tgccgagaac
     1681 atcgtcttca cgcatgatca agtaatcagc gccgtcgatt ttaatctcgg ttccagaata
     1741 tttgccaaag aggatcttat cgcccttttt gacttctaaa gagatgagtt tgccgtcttc
     1801 agtgcggcgg ccagcgccca cagccattac ttggccttca gctggttttt cttttgctgt
     1861 atcagggatg ataattccac ccttcgtttt ttcttcctcg ctcaaacgct ttacgaggat
     1921 gcggtcatgc aatggtttta cctgcatttt aaattcctcc ttctttctac attcaattta
     1981 acgaaaaaca tggcgccaac acgcgctgca tgagctcgcg ctaccccgtg aatttctcac
     2041 tgaatttcat ggtttgggcg aacactagca gaatcatatt gga
//
LOCUS       DAOMXO010000443         2074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31470_length_2074_cov_4.444279, whole genome shotgun sequence.
ACCESSION   DAOMXO010000443 DAOMXO010000000
VERSION     DAOMXO010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31470_length_2074_cov_4.444279"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLH57_08920"
     CDS             <1..839
                     /locus_tag="PLH57_08920"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRK02773.1"
                     /translation="LDEIIMEKMGIWTPRPDFSEWERLVYKVKNPSRHVKIGVVGKYV
                     DLKESYKSLSEALIHGGLANDAKVEIVYVDSEELQQGKGVQLLEQVDGILIPGGFGER
                     GIEGKIKAVEYARLHKKPFFGICLGLQLAVIEFARNVCGLKNATSYEFNPNDPCAVIT
                     VMEDQKSITNKGGTMRLGGYDCVLRPGTRAYSAYQKEVVSERHRHRYEVNNEFRKELE
                     SKGLVISGVYRSPKAELVEMIELADHPWFVACQFHPEFKSKPLKPHPLFAAFVSASLK
                     RT"
     gene            853..1677
                     /gene="kdsA"
                     /locus_tag="PLH57_08925"
     CDS             853..1677
                     /gene="kdsA"
                     /locus_tag="PLH57_08925"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRK02774.1"
                     /translation="MKRNDRFIFICGPCVIESEGLLDEVAGFLKETLARVPAWDFYFK
                     ASFDKANRSAHDSFRGPGLERGLKMLSGIKKKFGVKVLTDVHLPEHCGPAAETVDALQ
                     IPAFLCRQTDLVIEAATQAAKNGKSLNIKKGQFLAPWDMGNVIEKVESTTNQKRPPWL
                     WLTERGTSFGYNNLVVDMTSFPIMRKLGVPVLFDATHSVQLPGGAQGGKVTGGRREMI
                     EPLARAATAAGIDGIFMETHPRPAEAKSDAANAFPLTELPRFIESIERIRELEHIS"
     gene            1674..>2074
                     /locus_tag="PLH57_08930"
     CDS             1674..>2074
                     /locus_tag="PLH57_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02775.1"
                     /translation="MTTEFRRSSWSTTDAVAQVWVDELIWKMLRCCAAEEELGTATIP
                     TASGSSVELYKTAHAVVAAHGLLGVADLVYGRQLLDLKDVGAYWVDDAEKSRKRSDIL
                     NHELNWLVSPKLGGRVLAIDSAGMRSLYASIS"
BASE COUNT          561 a          432 c          567 g          514 t
ORIGIN      
        1 gtctcgacga aattattatg gagaaaatgg gaatctggac tccgcgacca gatttcagcg
       61 aatgggaacg attggtttac aaagtaaaga acccaagtcg ccatgttaaa atcggcgttg
      121 ttggtaagta tgttgatcta aaagaaagct ataagagttt gagcgaggcg cttattcatg
      181 gaggactcgc caacgatgca aaagtggaaa ttgtatatgt cgactccgaa gaattacagc
      241 agggtaaggg cgtgcagttg ctcgagcaag tggacggtat cttaattcct ggcggatttg
      301 gtgagcgggg aattgaagga aaaattaaag cagtagagta tgctcgactg cacaaaaaac
      361 cgttcttcgg aatttgtctt ggccttcaat tagcagtcat cgagtttgcg agaaatgttt
      421 gtggtctaaa aaatgctacc agctacgaat ttaaccctaa cgacccgtgt gcggtaatta
      481 cagtaatgga agatcagaaa tcgatcacca acaagggtgg taccatgagg ctcggtggtt
      541 acgattgtgt cttgcgtcca gggactcgag cctattccgc ttatcagaaa gaagtagtct
      601 cggagcgcca ccgccatcga tatgaagtga ataatgagtt caggaaagaa ctcgagtcga
      661 aaggtttggt cataagtggg gtttaccgct cgccgaaggc tgagcttgta gaaatgatcg
      721 agcttgcaga tcatccgtgg ttcgtggcct gtcaatttca tccggagttt aaaagtaaac
      781 cactaaagcc gcacccgttg ttcgcggcct ttgtgagtgc atcgcttaaa agaacgtaga
      841 aagaggccgt ctttgaaaag aaacgatcgc tttattttca tatgtggccc gtgcgtgatc
      901 gagtcagagg gcctgctcga cgaggtagct ggatttttaa aagaaactct agcccgagtt
      961 ccggcttggg acttttactt taaagcgtct ttcgacaaag caaatcgtag tgctcacgat
     1021 tcctttagag ggccgggact cgaacgtggg ctcaaaatgt tgagcggaat aaaaaagaag
     1081 ttcggagtaa aagtcttaac agacgttcat ttgcccgaac attgtggtcc cgccgctgaa
     1141 acggtagatg cgcttcagat accggcattt ctttgtcgcc aaacggatct tgttattgaa
     1201 gccgccactc aggccgctaa aaatggaaaa tcgctaaata ttaaaaaagg gcagtttttg
     1261 gcgccttggg acatgggaaa tgtcatcgaa aaggttgaaa gtacaaccaa tcaaaaaagg
     1321 ccgccttggc tttggttaac cgagcgaggt accagtttcg gatacaataa cttagtagtc
     1381 gatatgacgt ctttcccgat aatgcgaaaa ctcggagtac cggtactttt tgatgccacc
     1441 cattcggtgc aactacccgg gggcgcgcaa ggcggaaaag taactggcgg gcgccgggaa
     1501 atgatcgaac ctctcgcgcg tgcggcgacg gcagctggta tcgatggaat ttttatggaa
     1561 actcatccga ggccagccga ggcgaaaagt gacgcggcaa acgcattccc gttgaccgaa
     1621 cttccgcgtt ttatcgaaag tattgaacgc attcgtgagt tggagcatat ttcgtgacca
     1681 ctgaatttcg gcgctcgagc tggtccacta ctgatgcggt ggcgcaggtt tgggtcgacg
     1741 agttaatctg gaaaatgttg cgatgttgcg ccgctgaaga agaattggga actgcgacca
     1801 tccctacggc aagcggtagc tccgttgaac tttataaaac agctcacgcg gttgttgcgg
     1861 ctcatggttt actgggagtt gccgatttgg tgtatggtcg acaactactt gatttaaagg
     1921 atgtcggtgc atactgggta gatgacgcag aaaaatctcg caagcggtcc gatattttaa
     1981 accacgagtt gaactggctc gtaagtccta agcttggggg ccgagtgctc gccatcgatt
     2041 cggcgggcat gagatcgctt tacgcaagta tttc
//
LOCUS       DAOMXO010000444         2074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31474_length_2074_cov_3.793462, whole genome shotgun sequence.
ACCESSION   DAOMXO010000444 DAOMXO010000000
VERSION     DAOMXO010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31474_length_2074_cov_3.793462"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PLH57_08935"
     CDS             <1..1044
                     /locus_tag="PLH57_08935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK02776.1"
                     /translation="FLSSWRDSAFIDDQARALGAEFEVRFFDCAHSFSIIPKGIQIRL
                     QARRIIRDCRQQAWVPDLIHAHVAYPAGVIGQLVKKEFGVPLVLTEHTGPLELLEPYF
                     GSETAFNAHFLNYDQIICVSRFLKNQLSKRVPTHDQISVLGNIVGKKCFDTEIKTEIA
                     QQSALYVGRLTEEKGIIELSEALKLVDGCLKFKFIVKVIGNGPLRSLLESTALELKNI
                     KLEFLPEGPSVVCETLNEVRFLLLPSRIETFSMVAAEALAMGRPVLGFDCGGPSDFVS
                     EKMGHLIRERDPKLFANKMLEMINMREWELTSDECQNRRHSIERRFSAEAIFKGLEQV
                     YLRTLRVGRPQVY"
     gene            1062..1439
                     /locus_tag="PLH57_08940"
     CDS             1062..1439
                     /locus_tag="PLH57_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02777.1"
                     /translation="MKLTMSDVQLKFLLIAVSVGVSLIAQSNLKATSPQLPNNFDQLM
                     NGNIKVQLWILSRAIFFSGLSLVISWYAYKKFGFLELMVAQSVIYFAALLVSYFVFHE
                     PVGLNKIGAVTLISMGVALFYWK"
     gene            1442..>2074
                     /gene="hisH"
                     /locus_tag="PLH57_08945"
     CDS             1442..>2074
                     /gene="hisH"
                     /locus_tag="PLH57_08945"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRK02778.1"
                     /translation="MSGDTVIIDYGVGNLGSIQNMLQRVGVRSLVSSNSEEIHGAQRL
                     IFPGVGAFDHGIKNLRARGLEPVLKEAVLSKRIPILGICVGMQLLANGSDEGAEAGLG
                     WIPGRVVKFSKVDPNMKIPHMGWNRVEPKSDLCLFRGLDSPEFEDPRFYFVHSYYFRC
                     ERPNDILGTTEYYVPFVSAIRHENIWGVQFHPEKSHKFGMKLFRNFIEVTG"
BASE COUNT          552 a          374 c          519 g          629 t
ORIGIN      
        1 tttctctcat cttggaggga ttctgctttt atcgatgacc aagcgcgggc attgggtgcg
       61 gaatttgaag ttaggttttt tgattgtgcg cattcatttt caataattcc gaaggggatt
      121 caaattcgtc ttcaggctcg aagaataatt agggattgtc ggcaacaggc ctgggtaccg
      181 gatttaattc acgctcacgt ggcttatcca gcgggcgtca tcggtcagct cgtgaaaaaa
      241 gaattcggtg taccgttagt ccttactgaa cacacgggac ctctcgaact gttggagccc
      301 tactttggtt cagagactgc atttaacgct cattttttaa attatgatca gataatatgt
      361 gtgagcaggt ttctaaaaaa tcaactttca aaacgcgttc ctacgcacga tcagatatcg
      421 gtactcggaa atattgtcgg aaaaaaatgt ttcgataccg aaataaaaac cgaaattgcg
      481 cagcagtctg cactctatgt cggacgactg actgaggaaa aaggaattat tgagcttagt
      541 gaggctctaa agttagtgga tgggtgccta aagtttaaat tcattgtgaa agtgattgga
      601 aacggacctc tgcgatctct tttagaaagc actgctttag aattaaaaaa tatcaaactg
      661 gagttcttac ccgagggtcc gtcagtggtg tgcgagactc taaatgaagt aagatttctc
      721 ttgttgccta gtcgtattga gaccttttcc atggtggcgg ctgaggcgtt ggcaatgggg
      781 cggccggttt tgggcttcga ttgcggaggc ccttctgatt ttgtctccga aaaaatgggt
      841 catttgattc gggagcgcga ccccaaatta ttcgcaaaca agatgttaga gatgataaat
      901 atgcgcgagt gggaactcac ttcggacgag tgtcagaatc ggcggcatag tattgaaaga
      961 agattttcag ccgaggcgat ttttaaggga ttagagcagg tttatttaag aacattgcgg
     1021 gtaggccgac ctcaggtgta ttgatttagg gggagtttaa attgaaactt acaatgtccg
     1081 atgtgcaatt aaagtttttg cttattgctg tttcagttgg ggtttcactc attgctcaga
     1141 gcaatttgaa agcaacctcc ccgcaacttc caaacaattt tgatcaatta atgaacggca
     1201 acattaaggt tcaattgtgg atacttagta gagccatatt tttttcaggc ctttcactgg
     1261 tgatttcttg gtatgcttac aagaagtttg gatttttaga gttaatggtt gctcagtcgg
     1321 tcatttattt tgcggcgctt ttggtcagtt acttcgtatt tcatgagcca gtaggtctta
     1381 ataaaattgg agcagttacg ttaattagca tgggcgtagc gcttttttat tggaaataaa
     1441 gatgtcgggc gacactgtaa ttatagacta tggagttgga aatttaggct caattcagaa
     1501 catgcttcag cgagtgggtg tacgctcgct cgtcagttcc aattctgaag aaattcatgg
     1561 tgcacaacgt ctaatttttc cgggagtcgg agcctttgac cacggtatta aaaatctgcg
     1621 agcaagaggg ctggagccag tattaaaaga ggcagttttg agcaagcgta ttccaattct
     1681 tggtatttgc gtgggaatgc aacttttagc aaatggcagc gacgaaggag ccgaggcggg
     1741 cctgggttgg attcctgggc gtgttgtaaa attttcaaag gtagatccga acatgaagat
     1801 tccgcacatg ggctggaacc gagtagaacc caaaagtgat ctgtgcctgt tccgtggtct
     1861 agattcacct gagtttgaag accctcgttt ttatttcgta cattcgtatt acttccgatg
     1921 tgaacgccca aacgatattt tgggtactac agaatattat gttccctttg tttcagcgat
     1981 tcgtcatgaa aatatatggg gcgtgcagtt ccatcccgaa aaaagccata agtttggcat
     2041 gaagttattt agaaatttta ttgaagtgac tggg
//
LOCUS       DAOMXO010000445         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31513_length_2072_cov_5.083292, whole genome shotgun sequence.
ACCESSION   DAOMXO010000445 DAOMXO010000000
VERSION     DAOMXO010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31513_length_2072_cov_5.083292"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLH57_08950"
     CDS             complement(<1..454)
                     /locus_tag="PLH57_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRK02779.1"
                     /translation="MVRASDLIDQTFHAYNAARLREACQVFTQSMLKPDVTIGMSLTG
                     ALTPAGIGRSCLIPLVRAGFVDWIISTGANLYHDAHFGLKKKLVRGNPNIDDRLLRKE
                     GVIRIYDVLFDYDVLLSTDAFFREIIQHEDFLSPVRAARAACRRATGRD"
     gene            651..1955
                     /locus_tag="PLH57_08955"
     CDS             651..1955
                     /locus_tag="PLH57_08955"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRK02780.1"
                     /translation="MEIRDVIHGVVEVSSIEAQIVDSPFFQRLRNIKQLGFAENSYPG
                     ATHNRYSHSIGAKYLAQTAFRTIFGGRAGLLKRRPKTAERFEAVLRCAAMLHDIGHGP
                     LSHTTEFAMPKVDKLGLAQLACLPGKACVFSGSRQADHEDYTIKIILQSSLTAKLRRA
                     LGPLGAEPIHIACVINEDIYCPDSFFVDEGIDYRPVLHQLVSSEIDVDRMDYLTRDSY
                     YAGVNYGNFDLQWLLSHLTSHVHRNKCYLALGHKAIYAFDDFLLSRYHMFLNVYFHHK
                     SVIFDEMLSRYFKSSDCDYEIPSDIESYIFYDDYHLYTHLSKSTNSWAKRIYHKNPFK
                     MAAEFHSGIPQGKDHAREQKEELDRTIQGFKNRGIDYIVKTTTGELSKYFRRNERVPI
                     FVHYTSGIGNEKFYPLEECTDLFQKYANTRSITRVYTPKDPS"
BASE COUNT          550 a          471 c          467 g          584 t
ORIGIN      
        1 catctcgccc agttgctcgc cgacatgcgg cgcgtgctgc tctgaccggg gacagaaaat
       61 cttcgtgttg aatgatttct cgaaaaaaag cgtcagtcga aagcaatacg tcgtagtcaa
      121 agagtacatc gtaaatgcga ataacgcctt ctttacgaag taggcgatcg tcgatattcg
      181 gattgcctcg cactagtttt ttcttaagac caaaatgtgc gtcgtgataa agattggcgc
      241 ctgtactgat aatccagtcc acaaaaccgg cgcgtaccaa cggaatcaag caagaacgac
      301 ctataccagc cggagttaga gctcccgtaa ggctcattcc gatcgtaacg tcgggtttga
      361 gcatagattg agtaaatacc tggcaagcct ctcttagtcg cgcggcgtta taggcgtgaa
      421 atgtttgatc gatgagatcc gacgcccgaa ccatgcgtcg aatgccgtcg gggagaattt
      481 gtttgccttg aagaaaacga cttttcgatt tgccttcaat ggctcgaccg ccgcggtttt
      541 tttcagatat tgcggtcaat tgacgtttgg ttctgctcat aacaacgcat tgctccgatc
      601 ttggtgttta acttgctcgc acgtagcttg ctctgtatac cgggttttgt atggaaattc
      661 gagatgtcat tcacggagtc gtagaggtaa gttcgattga ggcccaaatt gtcgattcac
      721 ctttttttca gcgccttcga aatattaaac agctgggatt cgctgaaaat agctatcccg
      781 gtgcgaccca caatcgatac agtcacagca ttggtgcaaa gtatcttgcg caaactgcat
      841 ttcgaactat tttcggcgga cgagctggcc tgctgaagcg acgcccgaaa actgcagagc
      901 gtttcgaggc ggttctgcga tgtgcggcca tgctgcatga tattggtcat ggtccgcttt
      961 cgcacactac ggaatttgct atgcccaagg tcgacaaatt ggggttagcg caactcgcct
     1021 gtttaccagg aaaagcgtgt gttttttccg gtagtcgaca agccgatcac gaagactaca
     1081 ctatcaaaat tattttgcag agctcgctca ctgctaagtt gcgacgcgcg ttagggccgc
     1141 tcggtgcgga accaattcac attgcgtgcg ttataaacga agacatttat tgtcctgatt
     1201 cattttttgt cgacgaagga attgattatc gaccagttct gcatcaacta gtatccagtg
     1261 aaatcgatgt cgatcgtatg gattatctta cgcgcgactc ctattacgcc ggagtaaact
     1321 acggaaactt tgatttgcaa tggctgttgt cccatttaac ttcgcatgtg catcgcaata
     1381 aatgttattt agcgttggga cataaagcaa tttatgcttt cgacgatttt ttattgtcgc
     1441 gctaccacat gttcttgaac gtttattttc atcacaaaag tgtgattttc gatgaaatgc
     1501 tttcgcgcta ttttaagtct tcagactgcg actacgaaat tccaagtgat atagagtcgt
     1561 acattttcta cgacgactat catctgtaca cccatctttc gaaatcaacc aattcgtggg
     1621 ccaaacgtat ttatcacaaa aatccgttta agatggccgc ggagtttcac tccggaatac
     1681 cccaaggtaa agatcacgcg cgagagcaaa aagaggaact cgatcgcacc atacaagggt
     1741 ttaaaaaccg tggcattgat tacatcgtaa aaactaccac tggtgaactt tcaaaatact
     1801 ttcggcgcaa tgagcgcgtg ccaatttttg tgcattacac gagcggaatc ggaaacgaga
     1861 aattttaccc ccttgaagag tgcacagatc tgtttcaaaa atacgcaaat actcgttcaa
     1921 ttacgcgggt ctacaccccc aaagacccta gctaggccac gttaaaagtt aaaatttctg
     1981 aacttttatt tatatctatt tgatattgtt gatgtaattt taactttagc gaattgagct
     2041 aacgcccgtt gcctcttaca atgggagttg at
//
LOCUS       DAOMXO010000446         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31639_length_2066_cov_4.866733, whole genome shotgun sequence.
ACCESSION   DAOMXO010000446 DAOMXO010000000
VERSION     DAOMXO010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31639_length_2066_cov_4.866733"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLH57_08960"
     CDS             complement(<1..329)
                     /locus_tag="PLH57_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02781.1"
                     /translation="MVNLQRSRQQASMLRSSWWSWATLRGPNWTPSTTFSWAVAVTCF
                     ISCSLAFANESQTPKFKSTRDILLETYKDSPNADCKSVLNSNSNNLTLSYENIDERIR
                     IQHRFAV"
     gene            complement(442..1344)
                     /locus_tag="PLH57_08965"
     CDS             complement(442..1344)
                     /locus_tag="PLH57_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02782.1"
                     /translation="MLINPGGKTTLAPGAIGRVCSWGIFVLFLFNVGTGYAASYGDAN
                     WENSLLECGDCWGISGVDLGRTLPVVNRWLANVCLEDAKLERTTSKPIMVQEDDYSLC
                     KKGGFFSKTTHLASKTLYWISASTEAIHLHMRLRLAFGPKKLAHDRAATMTDRLEDCL
                     REASEVWARYGINLDIAVDWKADPVFEGAVSPITYVDDYGRSNSETFYFRGFPLKKGE
                     KPRTEKVRQAGFCTMVVHELGHLLGLDDEYEEVQACPDRPFLAERTEPPYSFMERNQS
                     NRQELEFFPRHVARVISPLCKWSK"
     gene            complement(1366..1629)
                     /locus_tag="PLH57_08970"
     CDS             complement(1366..1629)
                     /locus_tag="PLH57_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02783.1"
                     /translation="MDREAKLDLMRRALGIKHKLKVHDSMPRAETHEQVAATELARGE
                     LEEELAAIDELLFEARAEAIAEKKKEILKSGVKVKKLESSKED"
     gene            1730..>2066
                     /locus_tag="PLH57_08975"
     CDS             1730..>2066
                     /locus_tag="PLH57_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRK02784.1"
                     /translation="MMKTLVRVMGSRSLLAILVALVAPAAGASGSEAGHGHATGTKLS
                     PDEAIAEMKRGNERFVKGELVSAKADLKVRKSLVGGQKPHTIVLSCSDSRVAPELVFD
                     KGLGEIFPIR"
BASE COUNT          531 a          502 c          524 g          509 t
ORIGIN      
        1 cagactgcaa atcggtgctg aattcgaatc cgttcgtcga tattttcata agaaagagtc
       61 aaattgttag aattcgaatt cagcaccgat ttgcagtctg cattgggtga gtctttatag
      121 gtttcgagta ggatatctcg ggtagattta aatttaggag tttgagattc gtttgcgaat
      181 gcgagagagc aggaaataaa acaggtgacg gccacggccc aactgaacgt agtggaaggt
      241 gtccagtttg gccctcttag tgtggcccag ctccaccaac tggagcggag catcgacgct
      301 tgctgtcgcg atcgctgaag atttaccatt ttgctaagta ccccaaagtt aagtccaaaa
      361 atgaccaaaa ctaaagtaga cggctacttg gaaatacgcc tcagtgtcaa tacggagcgt
      421 taaaaaaaac cgaaaaagcg attatttcga ccacttacag aggggggaaa tgacccgtgc
      481 cacatggcgg gggaaaaact ctagctcctg acggttagac tgattgcgtt ccataaaact
      541 gtagggtggc tcggtacgtt ccgctagaaa cgggcggtcg ggacaggcct gaacctcttc
      601 atattcgtcg tctaatccta acaagtggcc cagctcatga actaccatcg tgcaaaaacc
      661 ggcctgccga actttttcgg tgcgtggttt ttcgcccttc ttcaaaggaa aacctcggaa
      721 ataaaaggtt tcggagttcg agcggccgta gtcgtccaca taggtgatcg gactaactgc
      781 accctcgaaa acggggtcgg ccttccagtc cactgctatg tcgaggttaa tgccgtagcg
      841 agcccaaacc tcagaagcct ctcttaggca atcttcaagg cgatcagtca tagtggcggc
      901 tctgtcatgt gcgagttttt taggaccaaa cgccaaccga aggcgcatat gtagatggat
      961 cgcttccgtc gaggcggaga tccaatataa cgttttacta gccaggtggg tagttttcga
     1021 aaaaaaaccg ccctttttac agaggctgta gtcgtcctcc tgaaccatga tcggtttgga
     1081 agttgtccgt tcaagttttg cgtcttcaag gcaaacattt gcaagccagc ggttgacgac
     1141 tggcaatgtg cggccgaggt ctaccccact gataccccaa cagtctccac actccagtag
     1201 cgaattttcc caattggcat cgccataact agctgcatag ccagtgccaa cgttaaatag
     1261 aaataaaacg aaaattcccc aactgcaaac gcgcccaatc gcaccaggtg cgagggtcgt
     1321 ttttccccct ggatttatga gcatttcccc ccggaaacgg ctggatcagt cttcttttga
     1381 cgactccagc tttttaactt taacgcctga cttcaaaatc tctttctttt tttcggcaat
     1441 ggcttcggcc ctagcttcaa acaacagttc gtcgatcgca gcgagctctt cctctagctc
     1501 cccccgggct aattcggtgg ccgccacctg ctcgtgagtt tcggcgcggg gcatgctgtc
     1561 atgcactttg agtttgtgtt taattcccag agcccggcgc attaaatcta acttcgcttc
     1621 gcgatccata gtgaggacca ctaacacgaa ttgagtagct aggcaaaagt gggaagtgag
     1681 atgattttgc aatctaaagc cgcggtccga atgtcacgaa aagcaggaca tgatgaaaac
     1741 attggttcgt gtgatgggtt ccaggtcttt actggcaata ttggtggcat tggttgctcc
     1801 ggcggctggg gctagcggaa gtgaagcagg acatgggcac gcgaccggta ccaaacttag
     1861 tcccgacgaa gccattgccg aaatgaaaag gggcaacgaa cgtttcgtaa agggagaact
     1921 cgtttcagca aaggccgatc tcaaagtaag aaaatcattg gtgggagggc aaaagcctca
     1981 caccatcgtt ctttcttgtt ccgattcaag agtggcgccg gaactggtgt tcgataaggg
     2041 cctcggtgag attttcccaa ttcgag
//
LOCUS       DAOMXO010000447         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31660_length_2065_cov_4.329851, whole genome shotgun sequence.
ACCESSION   DAOMXO010000447 DAOMXO010000000
VERSION     DAOMXO010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31660_length_2065_cov_4.329851"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PLH57_08980"
     CDS             <1..1118
                     /locus_tag="PLH57_08980"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HRK02785.1"
                     /translation="RIARAAVVRASMQATHLSVSWTDDSWKKKIQPTFAAHSYVRSPA
                     AQGLGGHWHSRYIDIDRFLAESSLHAAEKTLSQKIFRLIAEAEAHAHGCPVNEVAFHE
                     VGTVDSILDVVMAARCIRLVAADDYFASPVKLGRGVVQIEHGTHAVPPPAAAWLVQQM
                     QTSETPRAIVRENVELSTPTGLAILRALDVKFLDQWPSGRVLATGSGAGTMDLGNYPN
                     IFRVTLVATEAKVSKTEQQINPTPARFEEDQVTEVVLNVDDQTAERTAWILESLMARG
                     ALDAWLTPITGKKGRLATQISVLCAAALANDLMEWILENSSSFGLRYRTWDRKILSRR
                     FEDRETELGTMRFKLGSDLQGRILKEKIEFEDWRKRQ"
     gene            1115..>2065
                     /locus_tag="PLH57_08985"
     CDS             1115..>2065
                     /locus_tag="PLH57_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02786.1"
                     /translation="MNLGPKDRREGSLFYLLVAFFCRVTYRSLMMLFRFHRSEAAMVL
                     AGCLLGTTQAGWATPPRGAAEIFAEHERGVNSPCGEILFVKKLAAELPALDAPTAQQL
                     REMIERHFEKHLRQLPLWIAEALANDQDPELRDAAFAALETTAKIPGNKLLERVVQVL
                     NQLPPKIAEVATQRLKNNFSSQHLSFLWPLSSKHRHLVSADLWKQWRVADQLVHGSSG
                     AATQKYVAPNDILTHLEQILTLPIDSPEFSSASELSLAKIEELFQTARDVGLAGMFVD
                     RARVLYSSREEVVRRLNYLYLQSSAIDRPLDLDFIPRSHEF"
BASE COUNT          497 a          505 c          567 g          496 t
ORIGIN      
        1 tgaggattgc gagagctgct gtagtgcggg cgtcgatgca ggcgacgcac ctaagcgtga
       61 gttggaccga cgattcgtgg aaaaagaaga ttcaaccaac gtttgccgct cattcctacg
      121 tgcgttcccc cgcagcgcag ggactcggcg gccattggca ttcgcgttac atcgatatcg
      181 accggttttt agcggagtcc tcgttgcatg cggcggaaaa aacgctttcc caaaagattt
      241 ttaggctcat cgcagaagcc gaagcccatg ctcacggatg cccggtaaac gaggtggcgt
      301 ttcatgaagt gggaaccgtt gattcgattc tagacgtcgt gatggccgcc cgctgcataa
      361 gacttgtagc agccgacgac tattttgcct cgcctgttaa gcttggacgt ggcgttgttc
      421 agatcgaaca tggaacgcac gcagtgccgc cgccagccgc tgcttggttg gtgcaacaaa
      481 tgcaaacttc ggaaactcct cgggcgatcg tccgggaaaa tgtggagctc tcaacgccga
      541 ctggccttgc aatcttgcgg gcgctcgacg tcaagttctt agaccagtgg ccgtcgggca
      601 gagtcttagc gaccgggtcg ggggcgggta cgatggacct tgggaactat cctaatattt
      661 tcagggtcac actcgtcgcg acggaggcaa aggtttctaa aaccgagcag caaataaacc
      721 caacgcccgc gcgcttcgaa gaagaccagg ttaccgaggt agttttaaac gtagacgatc
      781 agaccgccga acgtacggcg tggattctag agtcgctgat ggcgcgaggg gcactggatg
      841 cttggcttac accgatcacc ggtaaaaaag ggcgccttgc cacccaaatt tcggttttgt
      901 gtgcggcagc cttggcgaac gatctcatgg aatggatatt ggaaaacagt tctagcttcg
      961 gtttgcggta ccgtacttgg gatcgaaaaa ttctgagtcg ccgttttgag gatcgtgaaa
     1021 ctgaactggg caccatgcgc tttaaattag gaagcgatct tcaggggcgg attctcaagg
     1081 aaaaaattga attcgaggat tggcgaaaac gacaatgaat ctcggaccca aggacagaag
     1141 agagggttcg ttattttatt tgttagttgc ctttttttgt cgggtaacgt atcgctccct
     1201 catgatgttg tttaggtttc atcgcagtga agcggcaatg gtacttgcgg gttgcttgct
     1261 tggaaccact caggctggat gggccactcc gcctcgtggg gccgccgaga ttttcgccga
     1321 acacgaacgg ggagtgaaca gtccgtgcgg tgaaatatta ttcgttaaga agctggcggc
     1381 cgagctacct gcactcgacg ccccgacggc acaacagctc cgggaaatga tcgagcgtca
     1441 cttcgagaaa cacctaaggc agcttcctct ttggattgcc gaggcactcg caaatgatca
     1501 ggacccggaa ttgcgagatg ccgcgtttgc ggctttggaa actactgcaa aaattcccgg
     1561 aaacaagtta ttggaaaggg tggttcaggt gctcaatcag ctccccccta aaattgcaga
     1621 agtcgcaact caacgattaa aaaacaattt ttcttcgcaa cacttgagtt ttctctggcc
     1681 actttcgtcc aaacatcggc acttggtatc ggccgattta tggaaacagt ggcgagtagc
     1741 cgaccaactc gtacacggat cgagtggtgc tgcaacccag aaatatgtag ctccaaacga
     1801 cattctgact catttggaac aaatccttac attgccaatt gattctccgg aattcagttc
     1861 tgcctctgaa ctatcgttgg caaaaatcga agaacttttt cagacggcgc gagacgttgg
     1921 gctcgcggga atgtttgttg accgcgcacg cgttctttat tccagtcgag aagaagtcgt
     1981 gcgaagactc aattacctgt atttgcaaag tagcgcgatt gatcgccctc ttgatttaga
     2041 ttttattccg cgtagtcacg aattt
//
LOCUS       DAOMXO010000448         2061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31747_length_2061_cov_3.294118, whole genome shotgun sequence.
ACCESSION   DAOMXO010000448 DAOMXO010000000
VERSION     DAOMXO010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31747_length_2061_cov_3.294118"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PLH57_08990"
     CDS             complement(<1..908)
                     /locus_tag="PLH57_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011650572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRK02787.1"
                     /translation="MTSSFRFAVLGCGRIAPKHADSLKKIDGVSLVACADRLPDRAKS
                     YAEKNAIKAFDSLEELLSWGQFDVLSVCTPSGMHADHAVAAMNAGKHVVVEKPMALLL
                     EDADRMIEASIRNQVRLFVVKQNRYNLPVLALRQALDEGRFGKLVLGTTRVRWCRDQK
                     YYDQDEWRGTWAQDGGVFANQASHHIDLLEWCMGDVESVLAKTATHLVDIECEDTGLV
                     ILKFVSGALGLIEATTATRPKDLEGSLSILGERGSVEIAGFAVNEIRHFNFVDVRPED
                     VEVKNKFSSNPPSVYGFGHHNYYQNVV"
     gene            complement(905..>2061)
                     /locus_tag="PLH57_08995"
     CDS             complement(905..>2061)
                     /locus_tag="PLH57_08995"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRK02788.1"
                     /translation="DFMNREIKFQDLARLHRSIRSELNAAFDDVLESSAFIQGKYVRK
                     FEEDFSKFVGIPRCFGVANGTDALEIALKAIGISSGDSVLVPAMTFAATAEAVLNVGA
                     VPVFVDISPDTLCATERHFEEALGSTNAKAKAFISVPLHGLADRLPELADFAKTHRLE
                     WIEDCAQAHGALVQGRHVGGFGAAGTFSFYPGKNLGALGDAGAIVTNSGHIAERVVSL
                     RDHGRTEKYLHTSVGRNSRLDGLQAAFLSVKLKYLSEWTLKRRTLAQRYFENLEKAEQ
                     PLRLLARPKDEQNHVFHNFVVRIVDDSNPQTNKWRARVIELLGKSGIETGIHYPVALS
                     EQPAFKQFRRKTEQAERVSREVLSLPIDPLMSLDDVDYVSEVLIEAVRNL"
BASE COUNT          535 a          555 c          434 g          537 t
ORIGIN      
        1 actacgtttt gatagtaatt atgatggcca aacccatata cactcggggg attcgaactg
       61 aatttatttt ttacttcgac gtcttctggt cggacatcta caaaattaaa gtgcctaatt
      121 tcgttcaccg caaatccggc gatttcaacg ctcccgcgtt ctcctaaaat actaagacta
      181 ccttcaagat ccttcggacg ggtcgccgtg gtcgcttcta tgagaccgag cgctccacta
      241 acaaatttca atataacaag gccggtgtct tcgcactcta tgtcgactaa atgagttgca
      301 gttttcgcga gtactgattc cacgtcgccc atacaccact cgagcaaatc aatgtggtga
      361 cttgcttgat tagcgaatac cccaccgtct tgggcccatg ttcctcgcca ttcgtcttga
      421 tcgtaatatt tttgatcgcg acaccaacgc acgcgagttg ttccaagtac tagttttcca
      481 aatcgcccct catctaaagc ctgacgcaag gccaaaacgg ggaggttgta gcgattctgt
      541 ttcaccacaa aaagccgaac ttggttacgt attgaagcct cgatcatgcg atctgcgtct
      601 tccaaaagaa gtgccatggg tttttcaacc accacatgtt ttccggcatt catcgccgcc
      661 actgcgtgat ctgcatgcat gccacttgga gtgcatactg agagcacgtc aaactgaccc
      721 catgaaagca gttcctccag tgaatcaaaa gccttgatgg catttttttc ggcataggac
      781 ttagcacgat ctggaagccg atctgcgcaa gctacgaggc taactccatc aatttttttt
      841 aaactatcag cgtgtttcgg cgcaattcta ccacagccca atactgcaaa acgaaacgac
      901 gaggtcataa atttcgtacc gcctcgatta gaacttctga tacgtaatct acgtcgtcga
      961 gtgacataag cgggtcgata ggcagactca acacctcgcg acttacgcgc tcagcttgtt
     1021 cagtttttcg tcgaaattgt ttaaatgcgg gctgttcact caaggcgacc ggatagtgaa
     1081 tacccgtttc tatcccgctt ttcccaagca attcgattac ccgcgcccgc cacttgttcg
     1141 tctgcgggtt agaatcgtct acgattcgaa caacaaagtt atgaaaaacg tgattttgct
     1201 catccttggg acgagctagc aatcgaagcg gctgctcggc tttttccaag ttctcgaaat
     1261 aacgctgcgc gagtgttcgc cgttttagag tccactctga tagatatttt agttttactg
     1321 acaaaaatgc ggcctgaagg ccatctaacc tcgagttacg cccaacacta gtgtgcaaat
     1381 atttttcggt ccgaccgtga tctcttagtg aaacaactct ctctgcaata tgcccgctat
     1441 tggtcacaat cgctccggcg tcacccaatg cacctaggtt tttgccaggg taaaaactaa
     1501 aggtgcccgc ggcaccgaaa ccacctacgt ggcgcccttg aactagtgct ccgtgagcct
     1561 gtgcacaatc ttctatccat tctagccgat gagtctttgc aaaatccgcc aactcgggca
     1621 atcggtcggc caatccatgc aggggaacag aaataaacgc cttagctttc gcgttcgtac
     1681 ttccaagtgc ttcttcgaag tgacgctcag tcgcacagag cgtatcgggc gaaatatcca
     1741 caaaaaccgg tacagcacct acgttgagaa ccgcctctgc agtagctgca aatgtcattg
     1801 caggaaccaa taccgaatcg ccggaactga ttcctattgc cttcagggcg atttcaagcg
     1861 cgtcggtgcc gttggcaact ccaaaacaac gcggaattcc tacaaatttc gaaaaatcct
     1921 cttcaaactt tcgaacgtat ttaccctgaa taaaggcact ggactcaagc acatcgtcaa
     1981 acgccgcatt caattcgcta cgaatagaac gatgtaaacg ggcaaggtcc tgaaatttaa
     2041 tttcgcggtt catgaaatcc c
//
LOCUS       DAOMXO010000449         2058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31800_length_2058_cov_4.480779, whole genome shotgun sequence.
ACCESSION   DAOMXO010000449 DAOMXO010000000
VERSION     DAOMXO010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31800_length_2058_cov_4.480779"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2058)
                     /locus_tag="PLH57_09000"
     CDS             complement(<1..>2058)
                     /locus_tag="PLH57_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HRK02789.1"
                     /translation="YVVRKYGRDKVSQIITFGKLLARGVIRDVGRVMSLTYAEVDSLA
                     KLVPEELGITLEEAFAKEPRLRELTDKEPRVAKLFENARALEGLGRHASVHAAGVVIT
                     NRPLVEYCPLYVAKDKENETVVQFDKDFAEKIGLVKFDFLGLKTLTVIENAVKYVRET
                     QKIKNVPENQYFTIDAVNYEDPNVYALISSGDTDGVFQLESSGMKDLCMRVQPTNLED
                     ITAICALYRPGPLNSGMVDDYINRKHGRTEVKYELPQLKEVLEETYGVIVYQEQVMRV
                     ARVLAGYSLGEADILRRAMGKKKPEEMAKQKDRFVKGCEANQIATATADHIFDLLAKF
                     AEYGFNKSHSAAYGVLSYQTAYLKHYYPAQFLAALMATEMDNTDKITQYIADAKRRGI
                     EVHPPDVNASQKTFSVVPPQGQGTDGVVKTPDVQHENIRFGLEAIKGVGGVAVDAILA
                     ERTEKGPFTSFVDFCKRVNLRKVNKKVVECLVSTGAFDSIAEQNRATLLESMQAIMDY
                     ASSVQEEASLGQVSMFDQYQATGLKMESDTSHLFTPTPDWPESKRLQMEKQLVGFYVS
                     GHPMEKWWPLVRGFVHGDLEWLNTEFERRKALPLKERGVMVQRESFGPGAYRNEGPRW
                     DVVLCCMVAETREIITKKGTRMAFVELEDARGKLEAICFPEAYEQYGAMLKESIDQCV
                     PLI"
BASE COUNT          452 a          580 c          476 g          550 t
ORIGIN      
        1 ttattaacgg aacgcattgg tcgatcgact cttttaacat tgctccgtac tgttcgtatg
       61 cttccggaaa acaaatcgcc tctaacttac ctcgtgcgtc ctcgagctcc acaaaggcca
      121 tgcgtgtgcc ttttttagtt ataatttctc gagtctctgc aaccatgcaa cacaacacca
      181 cgtcccagcg cggcccttcg ttgcggtagg cgccgggacc aaaagactca cgttgcacca
      241 taacaccgcg ctctttgagt ggcagcgcct tacgacgttc aaactcggtg ttcaaccatt
      301 ctaggtcgcc gtgcacaaac ccacgcacca acggccacca tttttccatg gggtgccccg
      361 aaacgtaaaa acccacgagc tgtttttcca tttgcaaacg cttgctctcg ggccaatccg
      421 gcgtcggcgt aaataaatgg gaagtgtcgg actccatttt taaaccagtt gcctggtatt
      481 ggtcgaacat gctcacttgc ccgagactag cttcttcctg cacgctcgag gcgtaatcca
      541 tgatcgcttg catgctttca agcagtgttg cgcgattttg ttctgcgata ctgtcaaacg
      601 ccccggtgga caccaaacat tccacaactt ttttatttac cttgcgaagg ttcacgcgtt
      661 tgcaaaaatc gacgaaactc gtgaagggcc ctttttctgt tcgttcggcc aaaatcgcgt
      721 cgactgcaac cccaccaaca cctttgatcg cttcgagccc gaaacggatg ttttcatgct
      781 gcacgtccgg ggttttgacg acgccgtcag tcccttgtcc ctgagggggg accacactaa
      841 atgtcttttg gctggcgttg acgtccggcg ggtgaacttc aatgccgcgg cgtttggcgt
      901 cggcaatgta ttgagtgatc ttatcggtat tgtccatttc agtcgccatt aaggcggcca
      961 gaaactgggc tggataataa tgttttaaat aggccgtctg gtacgacaac actccatagg
     1021 cagcggagtg cgacttgtta aatccgtatt ccgcgaactt ggcgagcagg tcgaaaatgt
     1081 ggtccgcagt ggcggtggca atttgattcg cttcgcaacc ctttacgaag cgatctttct
     1141 gtttggccat ctcctctggt tttttcttac ccatcgcgcg acggagaata tcggcttcac
     1201 caagagagta cccggccaac acccgagcca cccgcatcac ctgttcttgg taaacgatca
     1261 caccataggt ctcttcaagt acttctttaa gttggggaag ctcgtatttt acttcggtgc
     1321 gcccgtgttt gcgattaatg tagtcgtcga ccattcccga attcaaaggg cccgggcgat
     1381 agagagcaca gatcgccgta atatcctcga gattcgttgg ctgcacgcgc atacaaaggt
     1441 ctttcatgcc cgagctttcg agctgaaaca ccccgtccgt gtccccgctc gaaatcaatg
     1501 cgtacacatt gggatcttcg taatttaccg catcgatcgt gaaatattga ttctccggta
     1561 cattcttgat tttctgagtt tcgcgcacat acttcaccgc gttctcgatg accgtaagcg
     1621 ttttgagccc caggaagtcg aacttcacga gaccaatttt ctcggcaaaa tctttgtcga
     1681 actgaactac tgtctcattc tctttgtcct tggccacata aagcgggcag tactccacca
     1741 gtgggcgatt tgtaatcacc acaccggccg cgtgcaccga cgcgtgacga ccaagcccct
     1801 cgagcgcccg cgcgttttca aaaagttttg cgactcgcgg ttctttatct gtgagttccc
     1861 gaagtcgagg ttctttggca aacgcttctt ctagcgttat tccaagttct tcgggtacta
     1921 gtttagccag cgaatccact tctgcgtatg tcaaactcat cactcggccc acgtcacgaa
     1981 tcactccgcg cgcgagtaat ttaccgaaag taatgatctg cgaaaccttg tcgcgtccgt
     2041 acttgcgtac gacgtaat
//
LOCUS       DAOMXO010000450         2058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31803_length_2058_cov_4.078882, whole genome shotgun sequence.
ACCESSION   DAOMXO010000450 DAOMXO010000000
VERSION     DAOMXO010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31803_length_2058_cov_4.078882"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..559)
                     /locus_tag="PLH57_09005"
     CDS             complement(113..559)
                     /locus_tag="PLH57_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRK02790.1"
                     /translation="MPSIKERISEDMKNAMKSGDKARLAAIRMLHSAVRKREIDDRTD
                     LDDAGVLKILATLVKQRQESIEQFKAGNRADLVEKETAELTLLQAYMPEQMSEAELIP
                     IVEAAIKEAGASTKKEMGNVMKVLMPKVAGKADGKLVNQLVQARLQ"
     gene            complement(645..839)
                     /gene="rpsU"
                     /locus_tag="PLH57_09010"
     CDS             complement(645..839)
                     /gene="rpsU"
                     /locus_tag="PLH57_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRK02791.1"
                     /translation="MPGIRINDGESFEGALKRFKKQCEKAGILSDVRKREHFEKPSVK
                     RKKKAIAARKRAMKKVRRFY"
     gene            complement(917..1993)
                     /locus_tag="PLH57_09015"
     CDS             complement(917..1993)
                     /locus_tag="PLH57_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02792.1"
                     /translation="MKNPVFLILLACGSVSVVSVARASENYLPGAEIESFSGTCQDYL
                     RLETKKKKAIFRRLHAALKYGDGLKLIENTIPLFRQRIGRALQVSQERWTESNVIDEN
                     AKKEVVESVVLERMGIPVTEDIDQEMPTFQNKIASHRPKKLRKRFVPMPPVPYAEAGV
                     LHTYGYLFSQIQTPYGQKSDRWLVHGLEEKLGLVPGSLSPFSERGEFLSNLTDALDQV
                     VAGQNEFTKPEMVLHEKIIWRTPPGSIDFYGETSSEIELRRAVLTTRVYQLTNATALS
                     NGSPLDRLVVYSMRFIEQSRERYVTAFPADADFAKRLTARVETEGLSIEGLGPRYNLF
                     IPQSWQQVFYEPVFEVRGFTKMRR"
BASE COUNT          505 a          516 c          431 g          606 t
ORIGIN      
        1 gcaaagcccc atgtgatttg caccagcttt acgaagtcgt acaaattcgc ctacgacttc
       61 ggcaatattg ctgcgttctt taagttgtcg aacgaaatcg ctcatctaga aactattgca
      121 agcgcgcttg caccaattga tttacaagtt ttccgtcggc cttacctgcg accttcggca
      181 taagtacctt cattacattg cccatttctt ttttggtcga agctccagcc tcttttatgg
      241 ctgcttcgac aatgggaatt aattccgctt cggacatttg ttccggcata taagcttgga
      301 gcaaagttaa ttctgccgtt tccttctcga ctaaatcggc acgattgcca gctttaaact
      361 gttcaatcga ttcttgtcgt tgtttgacca aggtggctaa aattttcaac acccctgcat
      421 cgtccaaatc ggttcgatcg tcgatctcac gcttgcgaac tgcagagtgc agcatgcgaa
      481 tggcggcaag cctggctttg tcgcctgact tcatggcgtt tttcatatct tctgaaatgc
      541 gttctttgat cgatggcata tttcctcaca agtgacggca aataaaaaaa gcgcgaccaa
      601 gttttatcaa aaaacccggc cgcgctctat gtgattgtgg tcaattaata aaagcgacga
      661 accttcttca tcgcacgctt gcgcgcagcg atagcttttt tcttgcgctt tacacttggt
      721 ttttcaaagt gttcacgttt gcgcacgtct gaaaggatgc ctgctttttc acactgtttt
      781 ttgaagcgct ttaacgcgcc ttcaaaggat tctccgtcat taatacgaat tcctggcata
      841 ccaatcacct cctctcagca aaaattattg ggtcgaaact cgccccagaa ctaacgaaca
      901 ttttttggca ctcaagtcaa cggcgcattt tagtgaaacc acgtacctca aacaccggtt
      961 cgtagaatac ttgctgccaa ctctgcggaa taaaaaggtt atatcgaggc cccagcccct
     1021 caatagatag gccttctgtt tcgacacggg cggtaaggcg cttggcaaag tccgcatcag
     1081 ccggaaaggc cgttacatag cgttcccgcg actgttcaat gaatcgcatg gaatacacca
     1141 ctaggcggtc tagcggcgac ccattcgata gcgcagttgc atttgtaagc tgatagaccc
     1201 tagtggttaa tactgctcgg cgtagttcga tttcagaaga tgtttcaccg taaaaatcga
     1261 tacttccggg cggagttctc caaataattt tttcgtgaag taccatttcg ggcttagtga
     1321 attcattttg accggcgacc acttggtcca gcgcgtctgt taaattactt agaaattctc
     1381 cccgttccga gaatggcgac aaagagcccg gcactaaacc tagtttttct tcgagtccat
     1441 gaactaacca acgatcgctt ttttgtccgt aaggtgtctg aatctggctg aataaatacc
     1501 cgtaggtatg caacactcct gcttcagcat agggaaccgg aggcatgggc acgaatctct
     1561 tccgtagttt ctttggacgg tgactcgcaa tcttgttctg aaacgttggc atttcttggt
     1621 cgatatcttc ggtcactgga attcccattc gttcaagtac cactgattct accacttctt
     1681 ttttcgcgtt ttcgtctatg acgtttgatt cggtccaacg ttcctgcgaa acttgcaaag
     1741 ctcgtcctat gcgttgacga aaaagaggaa tcgtattttc gatcagtttt agtccgtcgc
     1801 cgtatttcaa agcggcgtga agacgtcgga atatagcttt cttttttttg gtttcgagcc
     1861 gtaagtaatc ttggcaggtt cccgaaaaac tctctatttc tgcgcctggc aaatagtttt
     1921 ccgaagcccg agcgaccgac accactgaaa ctgaaccgca cgccaatagg attagaaaaa
     1981 ccgggttttt caccttttaa actccgcaaa ataatttcaa aaactgctcg ctcctattac
     2041 catactaaat tactctgg
//
LOCUS       DAOMXO010000451         2055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_31883_length_2055_cov_4.331500, whole genome shotgun sequence.
ACCESSION   DAOMXO010000451 DAOMXO010000000
VERSION     DAOMXO010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31883_length_2055_cov_4.331500"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="PLH57_09020"
     CDS             <1..1100
                     /locus_tag="PLH57_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRK02793.1"
                     /translation="SYIQNVKEMVAAVAKNYTGDHDPVVLIHGESGVGKEVVARYIHR
                     KVFDRDEKNKPFVAINCAAFPDHLLESELFGHEKGAFTGAANRKMGLFELANRGTLFL
                     DEIGEMDIKMQAKMLRVLQERTIRRVGGTADIKVNAHIVTATNRDLSKAVAEGKFRED
                     LFYRLNTIPLTVPPLRSRPEDIPLLADHFLKVLGTNRGKSFTGFSVAAVKAMAQYRWP
                     GNVRELKSVIERALILEKGPVLELSQLRQETLRATMREVASTESVGVVNDNNVHAMGG
                     TGTTPTAGTGSLAPTGPRLYAAVSEKGLANVRKEVDEDLTRDVLVRWLTETQGNVSAI
                     SRDLKLDRANLLRLLRRFEIDPDLFRHKKAS"
     gene            complement(1097..>2055)
                     /locus_tag="PLH57_09025"
     CDS             complement(1097..>2055)
                     /locus_tag="PLH57_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02794.1"
                     /translation="EFQVTPEYVTNFQAPMNEFLAHSLGAEKADEIWERKTSLVSDIG
                     EIQALLVSDLEKIFGPDPQLKDIRVTLLSQGGMHHVYEISVPDTIAADPRFPGWRILR
                     VQKAMRHRSPKTHPNESPAFLERANKQFADSDNNFYYSVIAREIGISYLAASNLWRLP
                     VLNITSDETFARRGWTATTKAVGHSVKTALTPGNGIFAPTLMQQARLSKLIRQATTRG
                     KAFATMSQRYFYSRLKGSHLSKMLDRKRYTDVTGWDVRMPDDLILVQLSNLEAGVLRH
                     QSNDWIVRLNVIRKNADGSVITNRDGSPVVAPVWFKVILRDF"
BASE COUNT          524 a          457 c          561 g          513 t
ORIGIN      
        1 cttcgtatat ccagaatgta aaagaaatgg tcgccgcagt cgccaaaaat tacactggag
       61 accatgaccc cgtcgtttta attcacggtg agagcggggt gggaaaggaa gtcgtagcgc
      121 gctatattca ccgtaaagta tttgaccgcg acgaaaaaaa taaacctttc gttgcaataa
      181 actgtgccgc gtttccggac cacttgctgg agagtgagtt gtttgggcat gaaaagggcg
      241 cctttactgg agcggctaat cgtaaaatgg gtttatttga actggctaat cgcggtacac
      301 tgtttctcga cgaaatcggc gaaatggaca taaaaatgca ggctaagatg ctgcgggtgt
      361 tgcaggaacg caccattagg agagttggcg gcacggcaga cataaaagta aatgcccata
      421 tcgttacggc aactaatcgc gatttatcca aagccgtagc cgaaggtaag ttccgcgaag
      481 atctcttcta ccggttaaac actattccgc ttaccgtacc acctctgcgg agccgcccgg
      541 aagatattcc gttgctcgcg gatcattttt taaaagtact gggcactaat agaggaaaat
      601 cgtttaccgg attttcagtg gcggcggtaa aagcgatggc tcagtatcgc tggcccggca
      661 acgtgcgtga actaaagagt gttatagagc gcgcacttat tttggaaaag ggcccggtac
      721 tcgagctctc gcagttgaga caggaaaccc tacgcgctac gatgcgcgaa gtagctagta
      781 ccgagtccgt cggggtcgtc aacgataaca atgttcacgc tatgggagga acaggaacaa
      841 ctcctacggc aggaactgga tcgcttgcac caaccgggcc gcgtctttat gcagccgtga
      901 gcgagaaggg gctcgccaac gttcgtaaag aagtagacga agacttaacg cgcgacgttc
      961 ttgtgcgatg gctgaccgaa actcaaggca atgtctcggc gatttctcgg gatctaaaac
     1021 tagatcgcgc gaatttgtta cgtttgctgc ggcgatttga aattgaccca gacttgtttc
     1081 gtcacaaaaa ggcgagttag aaatcgcgca gtatgacttt gaaccaaaca ggcgctacca
     1141 cgggtgatcc gtcgcggttg gtgatcacgg aaccatctgc atttttgcga attacgttga
     1201 ggcgcacgat ccagtcgttg ctttggtgtc taagcacgcc ggcctctaag ttagaaagtt
     1261 gcaccaagat aagatcatca ggcatacgga catcccaacc ggtaacatcg gtgtatcgtt
     1321 tacgatcgag cattttcgat aagtgggagc ctttgagtct agagtaaaaa tagcgttgag
     1381 acatggtggc aaaggctttt ccgcgtgtgg tggcctggcg tatgagtttc gaaagtctcg
     1441 cctgttgcat gagtgtgggt gcaaaaattc catttcctgg agtgagggcg gtttttacag
     1501 agtgacccac cgccttggtg gtagcagtcc aaccgcgacg ggcaaacgtt tcgtcgctgg
     1561 taatgttaag aactggcagg cgccagaggt ttgaagccgc taaataactt attccgattt
     1621 cacgtgcgat aacggagtag taaaaattat tgtcagagtc cgcaaattgt ttgttcgcgc
     1681 gctcgagaaa tgccggactt tcattcgggt gggttttggg cgagcggtgg cgcatggctt
     1741 tttgtacgcg gagaatgcgc caaccaggaa atcttggatc tgcagcgata gtatctggaa
     1801 ctgagatttc gtaaacgtga tgcattccgc cttggcttaa aagagtgact cgaatgtctt
     1861 ttagctgtgg gtcagggccg aatatttttt ccaggtccga tacgagcaga gcctgaattt
     1921 cgccaatatc gctaactaga ctggttttac gttcccagat ttcgtcggcc ttttcagcgc
     1981 ctaagctgtg agctagaaat tcgttcatag gagcctgaaa attcgtcaca tattctggcg
     2041 tgacttgaaa ttctt
//
LOCUS       DAOMXO010000452         2044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32143_length_2044_cov_3.904977, whole genome shotgun sequence.
ACCESSION   DAOMXO010000452 DAOMXO010000000
VERSION     DAOMXO010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32143_length_2044_cov_3.904977"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>479)
                     /locus_tag="PLH57_09030"
     CDS             complement(<1..>479)
                     /locus_tag="PLH57_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02795.1"
                     /translation="GGARLVGKTAAIGMGVGGTLGAGLGKAMSHLTETTPEDAAALEG
                     ENRKLRALVSRGLAQESELLSSNVALAEIKNQDPVVNGLITGVIGGLIEADIINNTVV
                     KNLDDIAQDSLKAEARSLEKNARRAAKNLPKARVVKYKTKTATLPQSILGGIGMQYK"
     assembly_gap    480..489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<490..>2044)
                     /locus_tag="PLH57_09035"
     CDS             complement(<490..>2044)
                     /locus_tag="PLH57_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02796.1"
                     /translation="LIEPILSANIAKNLIDDSPDEYLVQMAQGAVCVSGLFRRLQKLD
                     VILGGPRFKTKNYSTGTALLAEPVPESDFADVAFEATAQVATSLAGLGVLGMLGDQGM
                     MGSSQTFPDGETLYDVCSPSSKFWSTYNTGTASITIDGTNYSYFYSKKDTPKRGTYSV
                     GEFCKQIGAEYKAIADEINEIVGSYSVLSTPIGKNRAYFELTQKLVDANLEIPKNIKN
                     FEHGNDWNDPAKWVCGVSFNHPETGEKVSTPVYSRSGDFIYEKSTCAQSVPQDILDEI
                     RALNLPNLKYHALGIDYNSRKYRDNPENKSPSCMTRAKNSFNTKSEQLALAKAASEIV
                     TVNKTKVLSGIEDEILRVACGKEKVVASRNLSGPNDPLRPNEKLVAVDAVTTNTRNYK
                     RNKSYRADLIARTIDDSEKTGIRDEVLLRPELMSAFLNCKRRPFERLYTLSGLDVESA
                     KNLSQKMSCDGRDKYRDYACVLKSNIVDPAIADKDIRKAARAKLLADLGQALSFVEAV
                     TAPVLVPMG"
BASE COUNT          447 a          500 c          487 g          600 t
ORIGIN      
        1 tctttatact gcatgccgat gccaccgaga atgctctgag gtaaagtggc tgtcttggtc
       61 ttatatttta cgacacgagc tttaggcagg ttttttgctg cccttcgagc gttcttttcc
      121 aacgatcgag cttctgcttt caacgaatcc tgcgcaatgt catctaaatt tttgacaacg
      181 gtattgttga tgatatcggc ttctattagg cctccgataa cacccgtaat cagcccgttt
      241 acgacagggt cttggttctt tatctctgcg agcgccacgt tacttgaaag aagttcagac
      301 tcttgcgcta gccctcgtga gaccagtgca cggagtttgc gattttcgcc ctcgagggcg
      361 gcagcatcct ccggagtggt ttcggtgagg tgagacatcg ctttgcccag gccagcgcct
      421 aaagtgccgc ctacacccat tccaattgct gcggtcttcc cgacaagccg agcaccgccn
      481 nnnnnnnnna atcccatagg tacgagtacg ggagcagtca ccgcttcaac aaaggaaagc
      541 gcctgtccaa gatcggctag gagtttcgcc cgagcggctt ttctaatatc cttatccgct
      601 atcgccgggt ctacaatgtt ggattttaga acgcaggcgt aatctcgata cttgtccctt
      661 ccatcgcaac tcattttttg agacaggttc ttggccgatt cgacatctaa tccagagagg
      721 gtgtacaaac gctcaaatgg gcgccgttta cagtttagaa atgccgacat gagttcgggg
      781 cgtaataata cttcgtctct gatccctgtt ttttcggagt catctatcgt gcgggcaata
      841 aggtctgccc gataggactt gtttctcttg tagttgcgag tgttggtggt gacggcgtcg
      901 actgcgacca gcttttcgtt agggcgcagg gggtcgttag gtccagaaag attcctagac
      961 gcaactactt tttcttttcc acacgcaacc cttaaaatct cgtcttcgat tccactcagt
     1021 actttagttt tatttaccgt gacaatttca gaggctgcct tagcgagcgc taattgttcg
     1081 gacttcgtat tgaacgagtt ctttgcgcgg gtcatacagc ttgggctctt gttttctgga
     1141 ttgtctctat actttcgcga attatagtcg atccccaacg cgtggtattt taggttcgga
     1201 aggttgagtg ctcgaatttc gtctaagatg tcttgtggga ccgactgtgc gcaggtactt
     1261 ttttcataaa taaaatcgcc cgaccgtgag taaacagggg tgcttacttt ttcgccagtt
     1321 tcggggtggt taaatgaaac cccacacacc cactttgcag gatcgttcca gtcgtttccg
     1381 tgttcaaaat ttttgatgtt tttaggaatt tctaagttcg cgtcgaccaa tttttgcgtc
     1441 agctcaaaat acgcccgatt ttttcctatc ggagtagaaa gtaccgaata agaacctacg
     1501 atttcattga tttcgtcggc gattgccttg tactcggcgc caatctgttt gcagaactcg
     1561 cccacggagt atgtgcctcg tttaggagta tcttttttag agtaaaagta actgtaattt
     1621 gttccgtcta tggtaatcga ggctgtgccg gtgttgtacg tgctccagaa tttgctgctt
     1681 gggctacata cgtcataaag ggtctcaccg tccggaaagg tttgactact gcccatcata
     1741 ccctgatcgc caagcatgcc caaaacacct aggcctgcta aactcgtcgc tacttgggcg
     1801 gtggcttcga atgcaacgtc ggcaaaatca gactcaggta ctggctctgc caaaagagcc
     1861 gtgccggtag aataattctt cgtcttaaaa cgtggcccac ctaaaattac gtccaatttc
     1921 tgtagacgtc gaaaaagccc gctaacgcag actgcaccct gggccatttg gaccaggtac
     1981 tcgtcagggg aatcgtctat taaatttttt gcgatgtttg cgctcaggat cggctctatg
     2041 agtt
//
LOCUS       DAOMXO010000453         2044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32148_length_2044_cov_3.198089, whole genome shotgun sequence.
ACCESSION   DAOMXO010000453 DAOMXO010000000
VERSION     DAOMXO010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32148_length_2044_cov_3.198089"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLH57_09040"
     CDS             complement(<1..1115)
                     /locus_tag="PLH57_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02797.1"
                     /translation="MKSPCGEFNKFYFTILVLGAMSAANAMGADAKYSYHPALTFALG
                     KGVHTANPSSERLRCVNFKESAPYNDTAVETSVDFYLIRQTQDLHRVFNIDASANASV
                     LMFKGNSKYDLQSHFNSKSSSVTLAIEGHSVFAPVVADNIEIQPRYQKMLDDGKMDDF
                     ESFCGSRFVSSEKRVVRVAVLVTIDDVSSDDRVKLTIDNGGGAGLGPISASAKVHVDA
                     LIQDRKTSRKVKFRVVATGGTGLDSLEKVIPAALTASDPLDSIRNELGSFIKTFSFKN
                     AAPGEFETMGYPGFDVAKERLISKEKMKRLTKLVESYRMWDSVYQLMTNVTGNTASEE
                     YKAVRKLFGATAISKAKDEMDSLNEFLENTANTHAEC"
     gene            complement(1096..>2044)
                     /locus_tag="PLH57_09045"
     CDS             complement(1096..>2044)
                     /locus_tag="PLH57_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02798.1"
                     /translation="EEKKECRCDALHAFRSENSTGRCLEKKVLRDPQFGGFRTGVSRF
                     FDSNEVVEGGTIIPIWDSTLPQESEEIFRLGIGLLAADVNWGGRSMVQYSKNAPNLAE
                     TLVAAAFAYRLDGESMANPIATGAIPPALWEGNILFVAPALGAVEVLESLKENDRIDF
                     ESFKKPRRMANDPTYSDFLKGIETAYKVFRNTETFEFTEFKLKGLAGCAESCLLKMPL
                     EIIASDDLNSYLLVRYDYGAISYQGIHLSQTVGDITRRAVLVLNAAKTVSFLDLIEAR
                     AVPKTRKINEAHVVFDRYGNLVTKMQRQTLRSEYEKSLW"
BASE COUNT          531 a          533 c          430 g          550 t
ORIGIN      
        1 aaacactcag cgtgagtgtt cgcggtgttc tccaaaaact catttaagct atccatctcg
       61 tctttggcct ttgatatcgc ggttgcacca aacagttttc gaacggcctt atattcttcc
      121 gaagcggtat taccggtgac attcgtcata agttggtaga cggaatccca catccggtaa
      181 ctttcgacca acttcgtcaa acgcttcatt ttttctttgg atattaagcg ttccttcgcg
      241 acatcaaaac cgggataacc cattgtttca aactctcctg gagcggcatt tttaaaacta
      301 aaggttttaa taaaggaacc cagttcgttt ctgatcgaat cgagagggtc actcgcggtg
      361 agggcggctg gaatcacttt ttctagagaa tcgaggccag ttcctccagt tgccacaacc
      421 cgaaacttaa ctttacgact ggtttttcga tcttgaatca atgcgtctac gtgcactttc
      481 gccgatgcgc tgatcggacc aagtcctgcg ccaccgccat tgtcgatagt taacttcact
      541 cggtcgtccg atgagacatc atcgatcgtg accaaaacag ctaccctgac cactcgcttt
      601 tccgaactta cgaaacggga accgcaaaat gattcaaagt cgtccatttt cccgtcatcg
      661 agcattttct gataccgagg ttgaatctca atattatcgg cgacgactgg cgcgaaaacc
      721 gaatgcccct cgatagcaag ggtaacactg ctgcttttgg aattaaaatg agactgcaaa
      781 tcgtacttgg agttgccttt gaacatcaaa acactggcat tcgcggacgc gtctatattg
      841 aacactctgt gcaggtcctg ggtttggcga atcaagtaga aatcaacgct cgtttctacc
      901 gccgtatcgt tgtacggtgc agactcctta aaatttacgc atcgtagtcg ttccgaacta
      961 ggattggccg tatgcacgcc tttccctaac gcgaaggtaa gtgccgggtg ataactgtac
     1021 tttgcgtcag ctcccattgc gttcgcggca gacatggctc ccaaaaccaa tatcgtaaaa
     1081 taaaatttat taaactcacc acaaggactt ttcatactca ctcctaagcg tttggcgttg
     1141 cattttggta accaaattac cgtaacgatc aaatacaacg tgtgcttcat tgatttttct
     1201 cgtttttgga acagcccgag cttcgattag atccaaaaaa gacactgttt tcgctgcatt
     1261 tagaactaaa accgcacgtc tcgtgatatc cccaacggtt tgggaaaggt gaattccttg
     1321 atacgaaata gccccgtagt cgtagcgaac caaaaggtac gaatttagat cgtcgctggc
     1381 gataatttcc aatggcattt tcaataaaca actctcggca cacccagcta gtcctttcag
     1441 cttaaattcc gtaaactcaa aggtttcagt gttccgaaac accttgtacg cggtttcgat
     1501 tcctttcaag aaatctgaat aggtagggtc attagccata cggcggggtt tcttaaacga
     1561 ctcaaaatca attcggtcat tttccttgag gctctcgagg acttccacag ctcccaaggc
     1621 cggggcgaca aataggatat tcccttccca gagagctggg ggtatcgctc ctgtcgcgat
     1681 tggatttgcc attgattccc catcaagtcg gtaagcgaac gcggccgcga cgagtgtttc
     1741 agccaagttc ggtgcatttt tggaatactg caccatcgat cggccgcccc aattaacatc
     1801 cgccgccaac agcccaatac cgagcctaaa aatttcctcg gattcctggg ggagtgtgga
     1861 atcccagatc ggaataatcg ttcccccctc caccacttca ttcgaatcga aaaaacgaga
     1921 tacgcccgtt cgaaacccac caaactgcgg gtcgcgtaaa actttttttt ccagacatct
     1981 accagtagag ttctccgaac gaaacgcgtg caacgcatca cagcggcatt cctttttttc
     2041 ttca
//
LOCUS       DAOMXO010000454         2038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32287_length_2038_cov_4.872416, whole genome shotgun sequence.
ACCESSION   DAOMXO010000454 DAOMXO010000000
VERSION     DAOMXO010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32287_length_2038_cov_4.872416"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="PLH57_09050"
     CDS             complement(<1..2002)
                     /locus_tag="PLH57_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02799.1"
                     /translation="MKFASGEERYFPALSRDGIGDSATLPHLDMDQKELGHAPPYELI
                     PVGEYVSRSSADPRMLHRIQKELDHFEKVDAPFKGEFEYRLQRGARRLASDPERAQLL
                     VEEVSGDLRLQRTIDELNARLVDPSERAITEGPLSLIEIEEARRFLKKRAKLKDDLID
                     ALLDSGVVGRPGSYETIDVAAARIYGSGEKIAKGRPVIVIPRKGKSYSSNLEVIESVT
                     IATNPVSGKKVVKDIKKVRDTEVVRSRDLDEVVLVDAQEYFANPRLRLPKESKLRADY
                     DGILARQAIEKVKAAAKIAERAGGKKPAKAVYSDTHQELVVVRLKNGEVFVARNGRRA
                     IADQKWTFEVEGNLQQLDEATGYKKGYVIYDSAEVAEVIPVRKLRPIDAWTDPKNKDL
                     VDAFEKFDALPNRIRRARLRRAQILEDKLDAAKEAAKKRGETFTIEEQAKIEMQIARD
                     FEVIRKIEEEILKNPKSKRAKAIDAAHRAGEVSDPPKALTKKQLKEKDKILIEGGLTA
                     AERRALMDLGITGDELQSAFAPYVYINGVQYAKRYKDVIVVRTDGTIQLVNELGYVQG
                     DLFSLNLFEINPRPMDEVIVYGKGFTVKAGEFSIVGIDDPIVFTDPRFARVRTLLEER
                     TSIFGWAKSLNQEIVPPKSLTSEPVAFFPNAQGLQCDSAFGYE"
BASE COUNT          436 a          538 c          422 g          642 t
ORIGIN      
        1 tttcataacc aaacgcagaa tcacactgca ggccctgagc atttggaaaa aatgccacag
       61 gttcggatgt cagcgacttg ggcggaacaa tttcttgatt taaactttta gcccatccga
      121 atatactggt ccgttcttcg agcaacgttc gcactctcgc aaacctcggg tctgtaaaaa
      181 caattgggtc gtcaattccg acaatcgaaa attctcccgc cttaacggta aaaccttttc
      241 cataaacaat tacttcgtcc atcggccgcg gattaatttc aaataagttg agtgaaaata
      301 ggtcgccctg aacataaccc aactcgttta ctaactggat cgtgccgtcc gttcgcacca
      361 cgatgacgtc tttatatcgt ttggcatact gtacgccgtt aatgtaaacg tagggcgcga
      421 atgcactttg tagctcgtct cctgtaatac ctaggtccat caacgctctt cgttcggctg
      481 ccgtaagccc gccttcaatt aagattttgt ctttttcttt tagctgcttt tttgtaagcg
      541 ctttaggcgg gtccgaaact tcgccggctc tgtgtgcggc atcaatagcc ttcgctcttt
      601 tgcttttcgg attttttagg atctcttctt caattttccg gataacttca aagtccctgg
      661 ctatttgcat ttcaatcttg gcttgttctt caattgtgaa agtttcgccg cgtttttttg
      721 ctgcttcctt agctgcatct aatttgtctt ctagaatttg ggctctacgt aatctcgccc
      781 gtcggattcg attcggaagt gcatcaaatt tctcaaatgc atctaccagg tccttatttt
      841 tagggtcagt ccaagcgtct atggggcgta attttcgaac cggtattact tccgcaactt
      901 ctgcagaatc gtaaattaca tatccttttt tgtagcccgt ggcttcgtcg agttgttgaa
      961 gattaccttc gacttcaaaa gtccactttt ggtcagcaat tgcacgacgc ccgtttctag
     1021 caacgaagac ttcgccattt tttaagcgaa ctactactaa ttcctgatga gtgtcggaat
     1081 acactgcttt cgcgggtttc ttcccgcctg ctctttcggc aatttttgct gctgccttaa
     1141 ccttttctat cgcctgtcgg gcaagtattc cgtcgtagtc tgctcgtaac ttgctttctt
     1201 taggcagtcg tagacgcggg ttggcgaaat attcttgcgc atcgaccaaa actacttcgt
     1261 ctaggtctcg agatcgcacg acttcggtgt ctctaacctt cttgatatct tttactactt
     1321 tttttccaga aaccggattg gttgcaatcg ttacgctctc tataacttcc aggttactac
     1381 tgtaactctt cccctttcgt ggaatcacaa tgaccgggcg acctttcgcg atcttttccc
     1441 ccgaaccata gattctcgcg gccgctacat cgattgtttc gtagctaccc ggtcggccca
     1501 cgactccgct gtcgagcaac gcgtctatta gatcatcttt gagttttgcc cgcttcttta
     1561 gaaatcttcg cgcttcttcg atttcaatta aactgagcgg cccttcagtg atcgctcgct
     1621 cgctcggatc caccaggcgc gcgtttagtt cgtcgatcgt tcgctgcaat ctaagatcgc
     1681 ccgacacttc ttctaccagt agctgggccc gttcggggtc gctcgcaagg cgtcttgcac
     1741 ctcgttgcaa acggtactcg aactcgcctt taaacggtgc atcaactttt tcgaagtgat
     1801 cgagttcctt ttgaattcga tgcaacattc tgggatcggc actgctacga cttacgtatt
     1861 ctccaacagg tataagttca tagggcgggg cgtgacccag ttctttttgg tccatgtcaa
     1921 gatggggcaa cgtcgcggag tctccaatac cgtcgcgcga gagtgctgga aaataacgtt
     1981 cctctccact agcaaatttc acgatcacgc cacttgagtc attaattaaa acactctc
//
LOCUS       DAOMXO010000455         2036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32331_length_2036_cov_5.864210, whole genome shotgun sequence.
ACCESSION   DAOMXO010000455 DAOMXO010000000
VERSION     DAOMXO010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32331_length_2036_cov_5.864210"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1116
                     /locus_tag="PLH57_09055"
     CDS             214..1116
                     /locus_tag="PLH57_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02800.1"
                     /translation="MKISQIKKFGVVVCLLATAALSAGCPEKPGDDEGPVDWTTALKR
                     ELGCAKEQFFSNRYMRSVDDPFEIQGLIDRIKADAPIDKGIQRKLEDAARNADMFFDV
                     GLRELDHIAKRYAVASVIPAYLIADGTQDPAAASNCKPDLMTAVAELEKLVKDGKVFS
                     KSCRYKTVGFSIQKASDDSTKARDVTPRVENRVLELSNQIASCGLQLQMTGNAQWMDI
                     FVLNKYVRDGNMSGVSVTDPAEIYAGLKPVYDAMFKLLRQPTLSTADLAAVTSYRSSE
                     KLLLISLREALKRQADVAQTYLGK"
     gene            complement(1117..>2036)
                     /gene="nadA"
                     /locus_tag="PLH57_09060"
     CDS             complement(1117..>2036)
                     /gene="nadA"
                     /locus_tag="PLH57_09060"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012412656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRK02801.1"
                     /translation="KDLFSEIQALKKSKNAILLAHYYQDADIQDVADFVGDSLDLSKK
                     AAATDADIIVFAGVHFMAETAKILNPSKKVLIPDLKAGCSLADTCPPDRFRAFREKYP
                     DTVVVSYINCSAEIKALSDYICTSSNAEKIISAIPRDKKILFAPDKNLGRYLAEKLGR
                     DLTLWPGSCIVHEIFSEKKITQLKVRHPAALVIAHPECEEGVLKLADHIGSTSSLLKF
                     TQTSPAKQFIVVTEAGIIHQMQKASPDKEFIAGPPQNECSCNECPYMKLNTLEKVRDC
                     LLHEKPELTLSEDLRVRALVPMQRMLSLS"
BASE COUNT          515 a          448 c          513 g          560 t
ORIGIN      
        1 ccaagaaaaa agctaagaag aaaaagaagt aacttctgct ttggatggta gtataggttt
       61 tatcccgaag ggggagcagc gaaagcggct tccccttcgg gctttctagg gcccacgatt
      121 tggccccagt tcctggggct ttccaaataa taatctctct gttagacccc gaagtgcgat
      181 gcccgtgggc gtcgatttat tttgggggaa atcatgaaaa tcagtcaaat taagaagttt
      241 ggtgtcgtag tgtgcctttt ggccactgcg gcgttgtccg caggatgtcc agaaaagcca
      301 ggtgacgatg aaggtccggt agattggacg actgcgctta agcgtgagct gggttgcgcg
      361 aaagaacagt ttttttcaaa ccgctatatg cgaagtgtcg acgacccgtt cgaaattcag
      421 ggcttgattg atcgaatcaa agccgatgcc cctatcgaca aaggcattca acgcaaattg
      481 gaagatgcgg ctcgcaatgc cgacatgttt tttgatgttg gactacgaga gttggatcac
      541 attgctaagc gatacgcagt ggcgagtgtt attccggcct acttaattgc cgatggtact
      601 caagatcccg ctgctgccag taactgcaaa ccggatttaa tgacagcggt ggctgagctt
      661 gaaaagctag tgaaagacgg aaaggtcttt tcgaagagct gtcgatacaa aacggtaggc
      721 tttagtattc aaaaagcttc agatgactcc acaaaggcac gcgacgttac tcccagggta
      781 gaaaatcgcg tgttggagct ctcaaatcag attgcttctt gtggcctgca gcttcaaatg
      841 acgggcaatg ctcagtggat ggatattttc gtcctcaaca agtatgtgcg tgatggcaac
      901 atgagcggtg tttcggtaac cgatccggcc gagatttacg ccggtctgaa gccagtttac
      961 gatgcaatgt tcaaactatt gcgccagcca actctgtcga cggctgattt ggctgccgtt
     1021 acgtcttatc gttcttcaga gaagttgctc ctgatctctc tgcgcgaggc gctaaagcgt
     1081 caagcagatg tagctcagac ctatttgggc aaataatcaa gaaagtgaga gcattcgttg
     1141 cattggcacg agtgctctca ctctcaaatc ttctgatagt gttagctccg gtttttcatg
     1201 gagcaaacag tcccgcacct tttctagcgt attgagtttc atgtaagggc actcgttaca
     1261 ggaacattcg ttctgaggcg gtcccgcaat gaattctttg tcgggcgacg ctttttgcat
     1321 ttgatgaatt ataccggctt ccgtcaccac gatgaactgt ttggcaggcg aagtttgagt
     1381 gaatttcaat aaactcgagg tcgacccaat gtgatcggct agcttcaaca caccctcttc
     1441 gcattccgga tgcgcaatga cgagtgccgc agggtgtctt accttgagct gagtaatctt
     1501 tttttctgaa aaaatttcgt ggacgataca actgccaggc caaagagtga ggtcgcgacc
     1561 aagtttttcg gccaaatagc gacctaaatt cttgtcaggc gcgaagagaa tttttttgtc
     1621 tctaggaatc gccgaaataa tcttctctgc gttactactg gtgcaaatgt aatcgctaag
     1681 cgcttttatt tcagcagaac aattaatgta agatacgacg actgtgtctg gatatttttc
     1741 tctaaacgcc ctaaatcgat ctggtgggca ggtgtccgct agtgaacagc ccgccttaag
     1801 gtcgggaatc aatacttttt ttgatgggtt taaaattttt gctgtctcgg ccatgaagtg
     1861 aacgccggcg aacactataa tatcggcgtc cgtggctgcc gctttttttg ataaatcgag
     1921 gctatcgccc acgaaatcag caacgtcttg gatgtcagcg tcttggtaat agtgcgccaa
     1981 caaaatcgca tttttggatt ttttgagagc ttgaatttcc gaaaataaat ctttca
//
LOCUS       DAOMXO010000456         2030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32475_length_2030_cov_5.413671, whole genome shotgun sequence.
ACCESSION   DAOMXO010000456 DAOMXO010000000
VERSION     DAOMXO010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32475_length_2030_cov_5.413671"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..216)
                     /gene="rpmD"
                     /locus_tag="PLH57_09065"
     CDS             complement(16..216)
                     /gene="rpmD"
                     /locus_tag="PLH57_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRK02802.1"
                     /translation="MKAKSKGTITVRLKKGLSQCTVDQRSTVKGLGLRRREDSRTLEN
                     TSAVRGMIKKVLHLLDVTAETR"
     gene            complement(234..749)
                     /gene="rpsE"
                     /locus_tag="PLH57_09070"
     CDS             complement(234..749)
                     /gene="rpsE"
                     /locus_tag="PLH57_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRK02803.1"
                     /translation="MASQNFKGNNEDNEFEDRVVHINRCAKVVKGGRRFSFAAIVVVG
                     DKKGQVGIGLGKAGEVPEAIKKATKQARKNLVRVSLQGTTIPHEITGVFGASRVLMKP
                     APEGSGVIASSSVRAILECAGIQNILTKSLKRDNPHNVVKATLEGLKALRQIDEIIRV
                     RSKNSGIEATA"
     gene            complement(767..1144)
                     /gene="rplR"
                     /locus_tag="PLH57_09075"
     CDS             complement(767..1144)
                     /gene="rplR"
                     /locus_tag="PLH57_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033008681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRK02804.1"
                     /translation="MATKVRKHTDEKKVIRFKRKRRIRGTVFGTAEKPRFAVFKSNAN
                     LFVQLINDDGGATLVAASTCEKETRGKVKNTREGAKALGELIAKRALEKKIDRVVFDR
                     SGYLYHGKIKTLADAAREAGLKF"
     gene            complement(1147..1692)
                     /gene="rplF"
                     /locus_tag="PLH57_09080"
     CDS             complement(1147..1692)
                     /gene="rplF"
                     /locus_tag="PLH57_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRK02805.1"
                     /translation="MSRVGKQPIKLPSGVKAEVKDGIIKVEGPKGKLSYKMPLGLNVK
                     TEAGVLRLEADEKKAPNARSLHGLVRTLVHNMVHGVSQGFTKELEIVGVGYRAQTKGN
                     VLNLTLGYSHPIDYKLPEGIQAKVDANTKLTISGADKMLVGLVAAKIRSFKEPEPYQG
                     KGVRYTNETIIRKQGKAAGAK"
     gene            complement(1698..>2030)
                     /gene="rpsH"
                     /locus_tag="PLH57_09085"
     CDS             complement(1698..>2030)
                     /gene="rpsH"
                     /locus_tag="PLH57_09085"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRK02806.1"
                     /translation="EKVDVPASRLKANIVKVLRDEGFVKSFRLVREEGRAWIKIQLRY
                     DDKGDAVVRGIRRVSRPGLRRYVGFEDVARVRSGAGVAIVSTSKGILTGEKAKAARVG
                     GEYLCEVW"
BASE COUNT          486 a          563 c          366 g          615 t
ORIGIN      
        1 catttcctac ctctactatc ttgtttctgc ggtcacatct aaaagatgta aaactttttt
       61 aatcattccg cgaaccgccg aagtattttc cagcgttcga gaatcctctc ttcgacgaag
      121 cccgagaccc ttgacggtag aacgttgatc caccgtacac tgtgagaggc ccttttttaa
      181 acgaacagta attgtacctt tgctcttagc cttcataaaa tgctcacact ttcttaagcg
      241 gttgcttcta tcccactatt cttggaacgc actcgaatga tctcatcaat ctgacgaagc
      301 gctttaagtc cttccaacgt cgctttcact acgttgtggg gattatcgcg tttcaaggac
      361 ttcgtaagaa tattctgaat tcctgcacat tctaatattg cgcgaaccga ggaactcgca
      421 ataacgcccg atccttccgg agccggtttc attaacacac gactcgctcc gaatacaccg
      481 gtgatctcat gtggaattgt cgtgccctgc aacgacactc gcactaaatt ctttcgcgcc
      541 tgtttcgtag ctttcttaat agcttcagga acttcgccgg cctttccaag tccgatacca
      601 acttgtccct ttttatcacc tacgacaacg attgcggcaa aactaaaacg acgtccgcct
      661 ttgaccactt tcgcacatcg attgatatgc actacgcgat cttcgaactc gttatcttcg
      721 ttatttccct taaaattttg gctagccaaa tgattatcct ccagacttag aatttcaagc
      781 cggcttctct agcagcatct gccagagttt taatttttcc gtgatacaaa tacccagatc
      841 gatcgaaaac cactcgatcg atttttttct ccaatgcccg ttttgcaatt aattcgccca
      901 aggctttcgc gccctcacgg gtgtttttta ccttgccacg agtctctttt tcgcaggtcg
      961 acgccgctac taaagttgcc ccgccgtcgt cattgatcaa ctgaacaaag agattcgcat
     1021 tgctcttaaa cactgcaaat cttggtttct ccgcagttcc aaaaacggtc ccacgaattc
     1081 ttcgctttct tttaaaacga atcacctttt tttcgtcggt gtgcttacgt actttagtag
     1141 ccattgttac ttcgctcccg ccgctttacc ttgcttacga atgatagttt cattcgtgta
     1201 tcgaacgcct tttccttgat atggctcagg ctctttaaac gatcgaattt tagcagcaac
     1261 taatccgacc aacattttat cagctcccga tatcgtcaac tttgtgttgg catcgacctt
     1321 cgcttgaatt ccttcaggca atttgtagtc gatggggtga gagtatccca acgtaaggtt
     1381 caaaacattt cccttagttt gggctcgata tcctacgcct acgatttcca attcctttgt
     1441 gaacccctgg ctaactccat gaaccatatt gtgcactaag gtccgcacaa gcccatgaag
     1501 tgatcgcgca ttaggagcct tcttttcgtc ggcctcaaga cgcagcactc cggcctcagt
     1561 tttaacgttg agacctaaag gcattttata cgacaactta cctttagggc cttcgacctt
     1621 aataattccg tcttttactt cggctttgac accagacggt aacttaattg gttgttttcc
     1681 tactcgtgac atacttttta ccaaacctca cacaggtatt cgccaccaac gcgagcggct
     1741 ttagcttttt caccggttaa gatgcccttc gaggtagata cgattgctac tccggctcca
     1801 ctgcgaacac gcgctacgtc ttcaaaacca acgtaacgtc gcaaaccggg gcgactaact
     1861 cgtcgaattc cgcgaacaac cgcgtctccc ttgtcatcat aacgaagctg gattttgatc
     1921 catgctcgcc cctcttctcg aacaagtcga aaagacttaa cgaaaccttc gtcccttaaa
     1981 acctttacaa tgtttgcttt caaacgagag gctggaacat ctactttttc
//
LOCUS       DAOMXO010000457         2021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32686_length_2021_cov_4.071719, whole genome shotgun sequence.
ACCESSION   DAOMXO010000457 DAOMXO010000000
VERSION     DAOMXO010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32686_length_2021_cov_4.071719"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1673)
                     /locus_tag="PLH57_09090"
     CDS             complement(210..1673)
                     /locus_tag="PLH57_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRK02807.1"
                     /translation="MSGEFSTHKSDNPFRHCNHTLVASDIHLTTAEPVHPKDKLWKRY
                     KQKEFFIDAAFEAFLLAVQKDVFGDKIELVLSGDIFDFDGVTQLPLRQKFFMTWYEKR
                     RGLDPEERKSRFKMKLIIEDHPIFFRSLKNFIKNGNRVIFIIGNHDLELHWDSVKRQV
                     LGALELNSEERERVRFNNWFYISNEDTLIEHGNQHDSHNNCQNPINPIISGRIKKRIR
                     LPFGSIANRFMMNGMGYFNPHSDRSYIMTFTQYLKFALKHLLKREPLIMWTWFWGAVA
                     TLYVTFREGLTPEVKDPLRVEDQVEEIAFNSNATPKIVRALKEIHAPAAIFDPLGVMR
                     ELWLDRAFIVAISAVIVFEIMTLINLIVPVSFFWTFALFALFIPFFIFYFKSIRSKVI
                     VPLRFYERAAKLGARITGVKRVIYGHTHEFMHTNFGGIEYLNSGTWSPAFDDIECTKP
                     SCPKTFVWIKPDPNDPEHKARVANVYQWKDHQIGLLC"
     gene            1689..>2021
                     /locus_tag="PLH57_09095"
     CDS             1689..>2021
                     /locus_tag="PLH57_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02808.1"
                     /translation="MMNSLLIILVAFSSVASIIAIFMALRQGRNDQSLELTRTLMEVS
                     AGLARSSAEIRQEVGEKLAQFGSDVQGKLDKNIRDGFSHFQKVQELLTNAEKQLSGLN
                     NVGKSIQDL"
BASE COUNT          540 a          436 c          486 g          559 t
ORIGIN      
        1 ggagccgttt aaacctagaa cgccaatttt tgccccgtag tagtaagaaa gataaatgtc
       61 tttcagaacc tgttttttcg gggggtgaac gcggcctacg ccctgcatgg tgcagataat
      121 ttgtttggaa ctcatgattg ctcctgtttt ccaggtgctt accacaaaat agctaggaat
      181 tatgagtaaa ttcaggtcgg aaacgaggtt tagcagagta agccgatttg atggtctttc
      241 cactgataga cgttggcaac ccgggccttg tgctcggggt cgttggggtc gggcttgatc
      301 cagacgaatg ttttggggca acttggtttg gtgcactcga tgtcgtcaaa cgccggggac
      361 caggtgccgg agtttaggta ttcgatgccg ccaaaattcg tgtgcatgaa ttcgtgtgta
      421 tggccataga tcacgcgttt tacacccgta attcgcgcgc ccagtttggc cgcgcgttcg
      481 taaaaccgca gcggtacaat cactttcgat cgaatcgact taaaataaaa aataaaaaac
      541 ggaataaata aggcaaacag tgcaaacgtc cagaaaaacg aaactggtac gatcaaattt
      601 attagcgtca taatttcgaa aacgataacg gcgctaattg ccacaatgaa tgcgcggtct
      661 aaccaaagtt cccgcataac cccgagcggg tcaaaaatag cagcgggtgc gtggatctct
      721 ttaagtgctc gaacaatttt gggagtcgcg ttggagttaa aagctatttc ttccacttgg
      781 tcttcgaccc gcaaagggtc cttaacttcc ggtgttagcc cttctcgaaa cgtcacgtaa
      841 agagtggcta cagcgcccca aaaccaggtc cacattatta ggggttctcg tttaagtaag
      901 tgtttcaggg cgaacttcag atattgagtg aaagtcatga tgtatgatcg atccgaatga
      961 gggttgaagt aacccattcc attcatcatg aaccgattag cgatacttcc gaacggaagg
     1021 cgaattcttt tcttaatccg accgctaatt atcggattga tggggttttg acagttgttg
     1081 tgagaatcgt gttggttccc gtgctcgatt agagtgtctt cgttgctaat ataaaaccag
     1141 ttgttaaagc gcactctctc gcgttcttct gagtttagct cgagcgcacc taatacctgc
     1201 cgcttgacgc tatcccagtg cagctcaaga tcgtggttgc ctataataaa aatgacgcga
     1261 ttgccgtttt tgataaaatt cttcaaagat ctaaaaaata ttggatgatc ttcgataatc
     1321 aatttcattt taaatcgaga ctttcgttct tcagggtcta ggccacggcg cttttcatac
     1381 caggtcatga aaaatttctg gcgcaacgga agttgggtaa cgccgtcgaa atcgaaaatg
     1441 tcgccagaaa gcactaattc gattttgtcg ccaaaaacat ctttttgcac cgcgagcaaa
     1501 aaggcttcga acgctgcgtc gataaaaaat tccttttgtt tgtagcgttt ccacaactta
     1561 tctttggggt gtaccggttc ggcggtagtt aagtgaatat cgcttgcgac caaggtgtgg
     1621 ttgcaatggc gaaaaggatt gtctgatttg tgcgtggaaa attccccgct catgtgggag
     1681 taacctctat gatgaactct ctattgatta tacttgtggc gtttagctct gtcgcttcca
     1741 ttattgctat ttttatggcc cttagacagg gtcgaaatga ccaaagttta gaactaacgc
     1801 ggaccctcat ggaagtttct gccggccttg ctcggtcgag cgccgagatc aggcaggaag
     1861 tcggcgaaaa gcttgcccaa tttggcagcg atgtgcaggg aaaactcgac aaaaacatac
     1921 gcgatggttt ttctcacttt caaaaagttc aggaactgct cacaaacgca gaaaaacaac
     1981 tctcgggttt aaataatgtc ggcaagtcaa ttcaagatct c
//
LOCUS       DAOMXO010000458         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32750_length_2019_cov_2.958758, whole genome shotgun sequence.
ACCESSION   DAOMXO010000458 DAOMXO010000000
VERSION     DAOMXO010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32750_length_2019_cov_2.958758"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..692)
                     /locus_tag="PLH57_09100"
     CDS             complement(180..692)
                     /locus_tag="PLH57_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRK02809.1"
                     /translation="MTFRVRRALLVVLSMTTLVFALAFSGCAAKKTKTGDEDSSGVAD
                     SDISGGMADSDSDKAMGLQTVNFAYDSSILDSWARNKLKENAKILRENSSLRVQIEGH
                     TDERGGIQYNLALGERRAGAARAYLIDQGIDADRMTVISYGKEKPVDPSSSEGAWAKN
                     RRANFRITSN"
     gene            complement(740..>2019)
                     /locus_tag="PLH57_09105"
     CDS             complement(740..>2019)
                     /locus_tag="PLH57_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HRK02810.1"
                     /translation="PFVLVLLLTLVTFALALWVTPAQPSQLLSFWNGGFWELLSFAMQ
                     MVLIVVTGEAIAETELVRNAIQRLCGIPKSAYSAVLLLTTVSILLGWLHWGFGLVAAS
                     LMGRELARNLHKRKIFVHYPILGTAAYMSMLLWHAGTTASAPLLINTPGHFLEKQIGL
                     VPLTETIFLPSNLLVCLILAITIPFVIAGLMPRRHYRTIDYFEIEEPKPIRASEEKGK
                     TIAEKLERSYSLNLTIGVLGLVFIAQYFRKNGLLGSNHNIVNLLLLSLGLVLHKSPLA
                     YARAVAQSVRGTSGIILHFPFYGGIMGLMRESGLGHEISTIFVQMATGETLPFFAYLA
                     SVVTKLFIPSGGGEWAVEGPVMLAAAKELSSSVGLTTMGIAYGNMVGNMFQPFWAIPL
                     LSIMGLKARDIMGYCLVVFVFAFPILGIALLMS"
BASE COUNT          562 a          514 c          457 g          486 t
ORIGIN      
        1 gctttgcgat agagttcggg cgctagagaa tctgcattga gatctttggc agctcgaatt
       61 gcggtttcag cgttggacat ttcctgtacg gggcgcgtag ccgcgacgga acaccccatc
      121 gcgaccacgc aaacccatac caaacgtaca gaaaattcaa agtgtctcag cactttttat
      181 taattcgaag taatcctaaa gttagcacgg cgatttttag cccaagcacc ttcgctgctc
      241 gaagggtcta ctggcttttc tttaccgtac gaaataactg tcatacgatc cgcgtcgatc
      301 ccttgatcga taagatacgc tcgcgcagct cctgcacgac gctcacccaa cgcaagattg
      361 tattgaattc cgcctcgttc gtcggtgtga ccttcgattt gtacacgaag cgagctattt
      421 tctctgagaa tcttagcgtt ctccttaagc ttattgcgcg cccacgaatc caggatcgac
      481 gagtcgtacg cgaaatttac agtttgaagg cccatggctt tgtcggaatc actgtcggcc
      541 attccacctg aaatgtccga atctgctacg ccgctggaat cttcatcgcc ggtttttgtt
      601 ttctttgcag cacaaccgct gaatgccaga gcgaatacta aagttgtcat ggataaaacc
      661 acgagcaatg ctcgccgtac cctaaaagtc atgatgaact ccttctgaga tttattttcg
      721 tattgtcgaa aagctagcac taggacatca acagtgcaat gccaagaatc gggaacgcaa
      781 aaacgaaaac gaccaaacaa tatcccatga tgtcgcgagc tttgagtccc atgatgctta
      841 gcagcggaat ggcccaaaaa ggttgaaaca tgtttccaac catattcccg taggcaattc
      901 ccatagtagt cagaccaacc gacgaactta gttccttagc ggcggccaac atgacgggtc
      961 cctcgaccgc ccattctccc cctcccgaag gaataaaaag tttggttacc acgctcgcga
     1021 gatacgcgaa aaaaggcagg gtttcacccg tagccatttg tacaaatata gttgatattt
     1081 cgtgccctaa ccccgactcc ctcataaggc ccatgatgcc gccatagaat gggaagtgta
     1141 atataatccc agaagtgccc cgcactgact gcgccaccgc tcgtgcgtaa gcgagcgggc
     1201 ttttatgaag gaccaaaccc aaactcagta acagtaggtt cacaatatta tgattagaac
     1261 cgagcaagcc attttttcga aaatactgtg caataaaaac caggcccaac accccgatgg
     1321 tgagattcaa agaatagctt cgctccaatt tttctgcgat ggttttaccc ttttcttcag
     1381 aggcgcgtat gggttttggt tcctcaatct cgaaataatc tatggttcgg taatgccgcc
     1441 taggcattag gcccgcgatt acaaacggta ttgtgattgc taatatgagg cacaccagca
     1501 aattgctcgg tagaaaaatg gtttcagtga gcggtactag gccaatttgt ttttctaaaa
     1561 agtgcccagg ggtattaatg agcaatggag ccgaagccgt cgtacctgcg tgccagagca
     1621 acatgctcat gtaggcggcc gtaccgagta ttggataatg aacgaaaatt tttcttttat
     1681 gtagatttcg tgccagctcc cgccccatca aactggcggc cacaagaccg aaaccccaat
     1741 gtagccaacc gagtaaaata gaaactgtcg tcagtagcaa caccgcggag taagctgact
     1801 tgggaattcc acacaatctt tgaatggcgt tacgcaccaa ttcagtttcc gcaatggcct
     1861 caccggtgac gacgatcaag accatctgca tggcgaaact aagaagttcc caaaaaccgc
     1921 cgttccaaaa gctaagtaac tgactgggtt gggcaggtgt aacccaaagg gcgagagcaa
     1981 aggtgacaag cgttaatagt aaaaccaata cgaaagggt
//
LOCUS       DAOMXO010000459         2016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32800_length_2016_cov_6.314635, whole genome shotgun sequence.
ACCESSION   DAOMXO010000459 DAOMXO010000000
VERSION     DAOMXO010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32800_length_2016_cov_6.314635"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLH57_09110"
     CDS             <1..226
                     /locus_tag="PLH57_09110"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRK02811.1"
                     /translation="KGDRMGNLIFRKAARNFNPMMATAGKITIVEVEELVDVGALDPD
                     QIHLPGIYVHRIFQGRAYEKRIEKKTVRAG"
     gene            232..891
                     /locus_tag="PLH57_09115"
     CDS             232..891
                     /locus_tag="PLH57_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRK02812.1"
                     /translation="MALTREQIAQRIALELRDGFFVNLGIGIPTLVANYIPKGMNVVL
                     QSENGLLGIGPYPRENEVDADLINAGKETVTTRAGSSFFSSAESFAMIRGGHVDLTVL
                     GAMEVDEHGNIANWMIPGKLVKGMGGAMDLVAGAKRVIVAMQHVAKDGSSKVLKNCKL
                     PLTGVKCVNRIVSELAEIEVTPRGLQLVARAPGVSVTEVRRATECELIVEGDVPEMTF
                     V"
     gene            complement(888..>2016)
                     /locus_tag="PLH57_09120"
     CDS             complement(888..>2016)
                     /locus_tag="PLH57_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02813.1"
                     /translation="WTYDSFIVMSYFPDLSTRTASILAALTAVAAGNLFGACSLEPIE
                     DTASAINIRIENEPPGLLAMLGDPFQINAPTQPSDFNCFAVNVTGDGITPSASIGGCV
                     SSDNFNGTGPGITSRHFLRGTAATIYVPAGDLRRVDVYGFYPSTPECGGSAGAVYSGY
                     FVGSKSIKLAEGGDVSIPISYSGANASVSCSEPEVNLIPTHVRQRNTTSSVCTFNSSA
                     PPTFPTPPSGPIIGPSDTNYLVEDGNLNVSECDTAPTETMDIQFRFDATGLDLSTFGR
                     MEVHWKGRGGTSGTGCNGSASQTLTTGGATAWLYEVSTDSWIQIGSASGTSVQLQGGD
                     FFQPLSRFVTSNYIFLRVVTPQLASGCAIIHTDVARIRLVR"
BASE COUNT          551 a          452 c          566 g          447 t
ORIGIN      
        1 gaagggcgat cgcatgggca atttgatatt tcgaaaagcc gcacgcaatt ttaatccgat
       61 gatggcgacg gctgggaaaa ttacgatcgt cgaagtggaa gaattagtag atgtgggagc
      121 gttagacccc gatcaaattc atttgcccgg aatatacgtg caccgtattt ttcaggggcg
      181 agcctatgaa aaaagaatag aaaagaaaac cgtaagggca gggtaaaaaa aatggcatta
      241 actcgagaac agatcgcgca acgaatagcc ctcgaactgc gagacggatt tttcgttaac
      301 cttggaattg gaatacctac attggtcgca aattacattc cgaaaggaat gaatgtggtt
      361 ttacagtccg aaaacgggct tcttgggatc ggaccctatc caagggaaaa tgaagtagat
      421 gcggatctca tcaacgcagg aaaggaaaca gtcacgaccc gcgcgggatc ctcttttttt
      481 tcgagcgctg agagtttcgc gatgattcgg ggtgggcacg tagacctcac cgtactgggc
      541 gccatggaag ttgacgaaca cggtaacata gcgaactgga tgattcccgg aaagctcgtt
      601 aaaggtatgg gtggggctat ggatctggtg gcaggagcca aacgagtcat cgttgcaatg
      661 caacacgtgg cgaaagatgg gtcgagcaag gtgttaaaaa actgtaaact accactcacg
      721 ggtgtaaagt gtgtgaatcg tattgtttca gaacttgcgg aaattgaagt gactccgcgc
      781 ggattgcagc tcgtggcccg agcacccgga gtttcggtta ccgaagttcg gcgcgcaacg
      841 gagtgtgaac tgattgtcga gggcgatgtt ccggaaatga cgtttgttta acgaaccagt
      901 cggatgcgcg cgacatcagt gtggatgatc gcgcagcctg aagccaactg tggagttacc
      961 actcgcagga atatataatt tgaagttaca aaccgactta agggttgaaa aaaatcgcct
     1021 ccctgtagtt gaacggaagt gccggaggcg cttccaattt gaatccaact atctgtcgat
     1081 acctcgtaca accaggcagt tgctccccca gttgtgaggg tctgactcgc acttccgttg
     1141 cacccggtgc cagaagtgcc gccgcggccc ttccaatgaa cctccatgcg accaaaggtc
     1201 gataaatcca atcctgtagc gtcgaaccga aattgaatgt ccatggtttc ggtcggcgcg
     1261 gtgtcacatt ctgaaacatt gagattgccg tcttcgacga ggtaattggt gtcagatggt
     1321 ccaatgattg ggcccgaggg tggagtcgga aacgttgggg gagcagagct gttaaaagtg
     1381 cagacggaac tcgtcgtatt gcgctgacga acatgagtgg gaatcagatt gacctctggt
     1441 tccgaacaac taactgacgc attggcgccg gaataggaaa tggggatcga aacgtcgcca
     1501 ccttcagcta atttaatgct ttttgatcca acaaaataac ccgagtaaac agcgcctgcg
     1561 ctaccgccgc actcaggagt cgatggataa aaaccgtaga catcaactct gcgaagatca
     1621 cccgccggta cgtatattgt cgcagcggtg cctcggagaa agtggcgaga agtaataccg
     1681 ggaccagttc cattaaagtt gtcgctcgaa acacacccac cgattgaagc gctgggcgta
     1741 attccgtcgc cggtaacatt gacggcaaag cagttaaagt cgctcggttg agtgggagcg
     1801 ttgatttgaa aaggatcgcc aagcatcgcc aaaaggcccg gcggctcatt ttcgattcgg
     1861 atgttaatcg cggaggcagt atcttcgatt ggttcgagcg aacaggctcc gaataagtta
     1921 cccgctgcaa ccgcggtaag tgccgcgaga atcgaagcag ttcgtgttga taagtccgga
     1981 aagtaactca tgacgatgaa tgaatcgtat gtccag
//
LOCUS       DAOMXO010000460         2015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32821_length_2015_cov_4.845408, whole genome shotgun sequence.
ACCESSION   DAOMXO010000460 DAOMXO010000000
VERSION     DAOMXO010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32821_length_2015_cov_4.845408"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLH57_09125"
     CDS             <1..605
                     /locus_tag="PLH57_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRK02814.1"
                     /translation="ASNVGFEVEWASEFLKEAEGQPIKNIGLETTRGSMRGELLVTQY
                     GLEGTPIYTLGISGPAWIDLFPDYTSEMIVEKFSKVREKLGPIRLVQRALRPQKTVQA
                     LLYHHGSLVENPMELARLLKRFPIKLNRSRPIDEAISTSGGVAWTEVDQNLMLKRYPG
                     VYLAGEMLSWDAPTGGYLIQGCVSLAAHVARAIKNQPKRS"
     gene            complement(580..1380)
                     /locus_tag="PLH57_09130"
     CDS             complement(580..1380)
                     /locus_tag="PLH57_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02815.1"
                     /translation="MILGILSFVFLATAHADTPTVAAPKEKHTVYVTPDHMFVPPGYD
                     STDVNVEVGLVGTLPNNCFELAGSPVVDHSRLDQGIIVLRQRAIYKPKLICTQISEPY
                     LQIANLGAEIPAGNYQIIVESSEKPTEPFTTAMTIAETETGDIDNFHYLNIQDAFLTK
                     KTVETVGQSEEETLLEDPKNKIKRFLNLRGEHRNVCVSLRDVDIKVFLKERIIQVLPI
                     VDLNQDPSCAKKVAEVVFSGELPKLPEGQYLVHIRSAFGKSKNVLVDF"
     gene            1451..>2015
                     /locus_tag="PLH57_09135"
     CDS             1451..>2015
                     /locus_tag="PLH57_09135"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HRK02816.1"
                     /translation="MSSKPNNATSYQIVVFSDGACKGNPGPGGWGAIVCIGDEVRELG
                     AGEALTTNNIMELTGVACALLFVERELARLLPKSAVAIQVYSDSKYVLQGASSWRNGW
                     KRAGWKKQDGTPVANLRQWQELDALIERLAVRASLSWTYVEGHAGIPGNERCDAIAEG
                     FALGHHVELYDGPADSYSVDLSRLVPTQ"
BASE COUNT          502 a          454 c          551 g          508 t
ORIGIN      
        1 gtgcctcgaa cgtagggttc gaagtggaat gggcatcaga gtttttaaaa gaggctgaag
       61 gtcagcccat aaaaaatatc ggtttggaaa cgacacgcgg gtcaatgcga ggcgaactcc
      121 tcgtaacgca atatggcctc gagggaacac ccatttatac gttggggatt tcgggaccag
      181 cttggattga tttgtttcca gattatacta gtgaaatgat cgtcgaaaaa tttagtaaag
      241 tacgcgaaaa acttggtccg attcgactgg tgcagcgcgc tcttcggcct cagaaaaccg
      301 ttcaagcgct gctttatcac cacggaagtt tggtcgagaa tcccatggaa ctagctcgct
      361 tgctcaaaag gttcccaatt aaattaaatc gcagccgacc gatcgacgag gccatttcca
      421 ctagcggtgg ggtggcgtgg accgaggtgg accagaactt aatgttaaag cgataccctg
      481 gcgtctacct cgccggagaa atgctctcat gggacgcccc gactggcggt tatttaattc
      541 aaggctgtgt gtcgcttgcc gcccacgtgg cgcgtgcgat taaaaatcaa ccaaaacgtt
      601 cttagatttt ccgaaggccg agcgaatatg aaccaagtac tgaccttccg gtagcttagg
      661 aagctcccca ctaaacacga cttcagcgac ttttttagca cacgaaggat cttggttaag
      721 atcgactatt ggtaaaacct gaatgatgcg ctcttttaga aaaactttaa tatctacgtc
      781 gcgaagactg acgcacacgt tgcggtgttc gccgcgcaga tttaaaaagc gtttaatttt
      841 gtttttagga tcttcgagaa gagtttcttc ttcggactga cccaccgttt ctaccgtttt
      901 tttcgtgaga aaggcgtctt gaatgttgag gtaatgaaaa ttgtcgatgt cgccggtttc
      961 ggtttcggca atggtcattg ctgttgtaaa gggttcagtc ggcttttcgg aggactcaac
     1021 aatgatctgg tagttgccgg ctggaatttc cgcgccaaga tttgcgattt gcaaataggg
     1081 ttctgaaatt tgagtacaga tcagtttagg tttgtagatc gcccgttggc gcaaaacgat
     1141 aataccctga tccaaacggg aatggtcgac caccgggcta ccggctagtt cgaagcagtt
     1201 gttcgggagt gttccgacga ggccaacctc gacattcacg tcagtgctgt cgtacccggg
     1261 gggcacgaac atgtggtctg gggtaacata aacggtgtgt ttttctttcg gtgccgcgac
     1321 agtcggagta tcggcatgag cggtcgccaa gaaaacgaaa ctaaggattc ccaatatcat
     1381 ggttaattcc cttctattca gttgaaatac tgttgaaaat tttggaccat cgtaacgaac
     1441 gaagtctctg atgtcgagta aaccaaataa cgcaacaagt tatcagatcg tcgtttttag
     1501 cgacggcgca tgcaaaggaa atcccggccc cggtggctgg ggggccattg tgtgtattgg
     1561 agacgaagta cgagaactcg gagcggggga agcacttacg accaataaca ttatggaact
     1621 cacgggggtg gcgtgcgcgc tactttttgt tgaacgcgaa ttagcgcgtc tgttgccaaa
     1681 gagtgctgtt gccattcagg tatattccga ctccaaatat gtgcttcagg gcgcctcttc
     1741 ttggcgcaac ggctggaaac gagcgggttg gaaaaaacaa gacggcactc ccgtggcaaa
     1801 tcttaggcag tggcaggagc tggacgcact tatcgagcgt ctggccgtgc gcgcaagttt
     1861 gagttggacc tacgtcgaag ggcacgccgg aattccgggt aatgaacggt gtgacgcgat
     1921 agctgaaggt tttgcactgg ggcaccacgt ggagctctat gatgggcccg cagattctta
     1981 ttctgtagat ctttcgaggc tcgtgccgac ccaac
//
LOCUS       DAOMXO010000461         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32938_length_2010_cov_6.372890, whole genome shotgun sequence.
ACCESSION   DAOMXO010000461 DAOMXO010000000
VERSION     DAOMXO010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32938_length_2010_cov_6.372890"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..944
                     /locus_tag="PLH57_09140"
     CDS             45..944
                     /locus_tag="PLH57_09140"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRK02817.1"
                     /translation="MRKAERFYILFGKHGQVGRRLLERVQSEIGAQVLAFDREEYDLA
                     GSSSELRSRLNQIDGQIEKFRNTHEIVALFTAAYTHVDGCELRPEFCRHVNVTNTIEV
                     LNHADRNWGAKIVFFSSDYVFDGRSGPYLETDVQRPLSVYGRAKKEVEDWIETHCADR
                     LILRTTGVFDYDRSSKNFVMQVLERLERGQAVHVVRDQYATPVWAKELSDATIDLVRA
                     GAVGVYNVAGGGPVINRVELARAVAQAFGYPTQNIVGISTASLRQPAARPKQGGLLSH
                     KTKQLIGWSPRSAAEALRILARK"
     gene            1051..>2010
                     /locus_tag="PLH57_09145"
     CDS             1051..>2010
                     /locus_tag="PLH57_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRK02818.1"
                     /translation="MSKRQALKTLPVASASHSTIHSPMEILNYQFEQAANLIKLEPNL
                     QQLLKEPYRELHVQVPLKHDNGELKIYHGYRIQHNAVRGPYKGGIRFHQDVDRNEVLA
                     LATLMTWKTAIVNIPFGGAKGGIAIDPTSLSSKEMQALTRGFTSKIDLIIGPHRDIPA
                     PDVNTDAKVMAWMMDEYGKKHGYTPAIVTGKPIALGGSAGRSEATGRGTVFTIVEACK
                     AYGIELSKATAVIQGFGNVGSFTAKFLRELGCKVLAVTDVKGGVFNANGLDIPALLKY
                     VQKEKTVVGFPGSEPISNDQIFYTKCDIVVPAALGGVLTGDVAR"
BASE COUNT          530 a          461 c          535 g          484 t
ORIGIN      
        1 gggagtttac ctgtctgcgc ctttaatata caaagagagc accaatgcgc aaagcggaac
       61 gattttatat tttatttggc aaacatggtc aggtaggccg acgcctcttg gagcgagtac
      121 aaagcgaaat cggtgcacaa gtgttggctt tcgaccgcga ggaatatgat cttgcgggtt
      181 ctagttcaga actacgttca cgattaaatc aaatcgatgg gcaaatagaa aaatttcgaa
      241 acacccatga aatagtagcc ttgtttaccg ccgcttacac ccatgtcgat gggtgtgaac
      301 tgcgccctga gttttgccga cacgtaaatg taaccaatac aatcgaggtg ttgaatcatg
      361 ccgatcgaaa ctggggagct aagattgtat tttttagctc cgattatgtt tttgatgggc
      421 gatctggccc ctatcttgaa accgatgtgc agcgtccgct tagcgtatat gggcgtgcaa
      481 aaaaagaagt tgaggattgg attgaaacac attgtgcgga ccgtttgata cttcgtacga
      541 caggagtatt cgattacgat cgttcgtcga aaaattttgt aatgcaggtc cttgaacggc
      601 tcgaacgagg tcaagcagtt cacgttgtgc gcgaccaata tgctacgccc gtttgggcca
      661 aggaactttc cgatgcgacc attgacctgg taagggctgg ggcggtggga gtgtataacg
      721 tcgcaggagg gggcccggtg atcaatcgag tagagctcgc tcgcgctgtg gctcaagcat
      781 ttggttatcc tactcaaaat atagtgggca tttccaccgc tagcttgagg caaccggctg
      841 cgcgcccgaa acaaggcggg cttttgagtc ataaaaccaa acagctaatc ggctggtctc
      901 cccgatctgc cgccgaggca ctacggattt tggcccgtaa gtagtggtaa acaccccagt
      961 ttaatttggc ttaagttcta ggacatttct gccgataggt aagagtgggc caaaagccct
     1021 gtttcaaact tgtcacgata ggggacaaaa atgtcgaagc gccaagccct gaaaacctta
     1081 ccggtcgcat ctgccagtca ctcaactatt cacagcccaa tggaaatttt gaattaccag
     1141 ttcgaacagg ctgcgaatct cattaagtta gagcctaatc ttcagcagtt gctcaaggaa
     1201 ccctatcgcg agctccatgt gcaggtgccg cttaaacacg ataatggcga attaaagatt
     1261 tatcacggct atcgtattca acacaacgca gtacgggggc cttacaaggg ggggattcgt
     1321 tttcaccaag atgttgaccg caatgaagtg ttggcattag cgaccctcat gacttggaaa
     1381 actgcgattg tgaacattcc gttcggcggt gccaaggggg ggattgcgat cgatccgacc
     1441 agtctttcga gcaaagaaat gcaagccttg actcgtggat ttacgtcgaa aatcgatttg
     1501 attatcgggc ctcaccgcga cattcccgcg ccagacgtaa ataccgacgc taaagtaatg
     1561 gcctggatga tggacgagta cggaaaaaaa cacggttaca ccccagccat tgttacagga
     1621 aaaccgattg cattgggcgg aagcgccggt cgaagtgaag cgactgggcg agggaccgta
     1681 tttacgattg tcgaggcctg caaagcttac gggatagaac tcagcaaggc cactgcggtg
     1741 attcagggct tcggcaatgt cggctcgttc acagctaaat ttctgcgcga acttggttgc
     1801 aaagtattgg cggttacaga cgtaaaaggc ggagtattca atgcaaacgg gctcgatatt
     1861 cccgccctgc ttaagtacgt tcagaaagaa aaaaccgtag ttggatttcc gggcagcgaa
     1921 cccatatcga acgatcaaat tttttatacc aagtgcgaca tcgtagttcc cgcggcgctt
     1981 ggcggggttt tgacgggtga cgttgcgcgc
//
LOCUS       DAOMXO010000462         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32951_length_2010_cov_3.073657, whole genome shotgun sequence.
ACCESSION   DAOMXO010000462 DAOMXO010000000
VERSION     DAOMXO010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32951_length_2010_cov_3.073657"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLH57_09150"
     CDS             complement(<1..844)
                     /locus_tag="PLH57_09150"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HRK02819.1"
                     /translation="MSTYSMTGFGRAEYKTSNLVFTAQVRSVNHRFLDIKVKLPRSEW
                     VALDHRVRKIVSQKCSRGSVEVSVSVAPLGSKHAAAQSLQLDTVDAFIQQIDHWKKQK
                     RKKRTWLKETVQLDTLLRMPGVFGNTVTSVLDQFTDEAKLLECLVEPALEELQKSRTS
                     EGSALQKHIRSLLDQLRIQLQAIIKLEPLEQSRVRDSLADRIKATLELLGKTNVSDDF
                     AKRLNEEAAFWIDRRGTEEERVRFEAHLVAFENTLQKETGPKGRKLEFLQQELHREIN
                     TLGNK"
     gene            complement(846..1838)
                     /locus_tag="PLH57_09155"
     CDS             complement(846..1838)
                     /locus_tag="PLH57_09155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRK02820.1"
                     /translation="MEAVFEFEKPIAALERRIAELRAMSQTDNVNFQSEIKGLEGKVH
                     ALIDEIYGTLSPWQRVQLSRHPGRPYALDYIPLLFSEFTELHGDRHFGDDPAIVCGLA
                     RFGKTPVAIIAQQKGRNTKQKVERNFGMGKPEGYRKSERIMELAHRFHLPIFTFIDTP
                     GAFPGMDAEERGQSEAIASAIEKMFEIESPIIATVIGEGGSGGALAIGVADRVLMQAY
                     STYSVISPESCASILWSDSSRQEQAAQIMKMLPEDLLRLGVVDAIIPEPKGGAHRSLE
                     EAATLLKKHLTKHLGEVLKSAKTPETLIKSRFEKFRRIGQSAIREATPTKTGGR"
BASE COUNT          481 a          488 c          520 g          521 t
ORIGIN      
        1 ttttgttacc cagggtgtta atttcacggt ggagttcctg ttggagaaac tctaactttc
       61 gacctttagg gcccgtctct ttttgaagtg tattttcaaa tgccaccaga tgggcttcaa
      121 aacgtacacg ttcttcttca gtgcctcgcc gatcgatcca aaacgcggct tcctcgttga
      181 ggcgtttcgc aaagtcgtcg gaaacgttgg tttttcctag tagctcgagc gtcgctttga
      241 tgcggtccgc aaggctatcg cgaacgcgac tttgttctaa tggttcgagt ttaatgattg
      301 cttgcaattg aatacgcagt tggtccaaca gagagcgaat atgtttttga agtgccgagc
      361 cttcactagt gcgagacttt tgtagttcct caagagctgg ttccactaaa cactcgagga
      421 gcttagcttc atccgtgaac tggtcgagta ccgaagtcac ggtattccca aaaacaccgg
      481 gcatgcgtaa aagcgtgtcg agttgcacgg tttccttgag ccaagtgcgc tttttacgct
      541 tttgtttttt ccaatgatca atttgttgaa tgaaggcatc aaccgtgtcg agttgcagac
      601 tttgagcggc ggcgtgtttc gacccgagag gggcaacgga aacgctcact tcgacagacc
      661 cgcgagaaca tttttgagac actatctttc gaactcggtg gtcgagggcc acccactctg
      721 atctcggaag tttaactttt atgtcgagaa atcggtgatt gaccgagcga acttgcgcgg
      781 taaacaccaa attagatgtt ttgtactccg cacgaccgaa tccagtcata gagtaggtgg
      841 acatattagc ggccacctgt tttggttggg gtcgcctcgc gaatggccga ttgaccaata
      901 cggcgaaatt tttcgaaacg tgacttaatg agggtttcgg gagtctttgc cgattttaaa
      961 acttcgccga gatgcttggt tagatgtttt tttagcaatg tagcggcttc ttcaagactg
     1021 cggtgagccc cgcctttcgg ctctgggata atggcatcga ctacccctaa tcgcagaaga
     1081 tcttcgggga gcattttcat aatctgagca gcctgttcct ggcgcgaaga gtctgaccat
     1141 agaatagagg cgcaggactc aggtgagatg actgagtaag tggaataggc ttgcatcaag
     1201 acgcgatccg ccaccccgat agccagggcc ccgccgcttc caccttctcc aataacagtt
     1261 gcgatgattg gactttcgat ttcaaacatt ttttcaatcg ccgatgcgat agcttcagat
     1321 tgaccgcgtt cctcagcgtc cattcccgga aacgcgcctg gtgtgtcgat gaaggtaaaa
     1381 atgggcaaat gaaaccggtg cgcgagttcc atgattcgtt ccgacttgcg atatccttcg
     1441 ggcttgccca ttccaaagtt gcgttcgact ttctgcttgg tgttgcgccc tttttgctga
     1501 gcgatgatcg caaccggagt ttttccaaat cgcgcaaggc cgcatacgat agcgggatcg
     1561 tctccaaaat ggcggtcccc atgaagctcg gtaaactcgc taaaaagtag ggggatatag
     1621 tctagagcgt acggccgccc tgggtggcgg gagagttgca ctcgttgcca aggggacaag
     1681 gtgccataaa tttcatctat gagagcgtga acctttcctt ccaaaccctt gatttcggat
     1741 tgaaagttaa cgttgtcagt ctgtgacatt gcgcgcagct cggcaatacg tctctcgagc
     1801 gcagcgattg gtttttcgaa ctcaaaaaca gcttccattt aagaaaaaca agctaacaaa
     1861 agccgaaggg ggagtaaagc gttgcaaaaa cctaaagcgc cgttaaactt gaagaaatat
     1921 ggccgaagct ccagcaaccc caccagatac cgcgaaaaaa gacgcaccac agcaatcggg
     1981 ggcgccagcc tctgctgatg cggcgggcgc
//
LOCUS       DAOMXO010000463         2009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_32963_length_2009_cov_5.625896, whole genome shotgun sequence.
ACCESSION   DAOMXO010000463 DAOMXO010000000
VERSION     DAOMXO010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32963_length_2009_cov_5.625896"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLH57_09160"
     CDS             <1..253
                     /locus_tag="PLH57_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02821.1"
                     /translation="AKDLIGSDDIKSNTVEFVLTKTYFENLGVLMQEYLTTTSVGDLI
                     RKARGHDVQKNVGTDTVRPMIAPEATRGETTTKLGRNLS"
     gene            216..1388
                     /locus_tag="PLH57_09165"
     CDS             216..1388
                     /locus_tag="PLH57_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRK02822.1"
                     /translation="MKRLRSLDAIYLDYNASAPFPTELNEEFSSVLLSGYGNPSSLHF
                     FGRKSRRLINEATEFVAKFVNADPAFCYWTSGASEANTWVLHSAVLRAKAEGRSPQLL
                     VSAIEHESTLLAAKHLASQFKDVRVDLLPVLRNGQIDLEALAQMLTQARYDLVSVLHA
                     NNETGVVQPVDQLTEICARANIPVHIDCVQTLAKIPFTIADRGIQFLTFSGHKIGAPK
                     GVGLLAVRGEGRLLYPLIHGKQQKGLRGGTENVTGVTMFARTLSAYQNGKIRSFEHCQ
                     TLHDRFEKQLTETIPGTIIHGLGAARLPNTTYVGFEGLDGDSLLMGLDLEGVCASSGS
                     ACSSGSLDPSHVLSAMGCTPELARSSVRFSSGPNTTWSEFETLLNVLSELVSRLRT"
     gene            1456..>2009
                     /locus_tag="PLH57_09170"
     CDS             1456..>2009
                     /locus_tag="PLH57_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02823.1"
                     /translation="MSLLRTFRLFSLGVLLLGLQFLSVASAADSEANEPNLEIPQDTT
                     PVSERTPVRIWIEADLLRSMTPEQREDVQKIIAIAEERLFTWKNFGFPEMPESMFSPD
                     EPAIVEIASLFMTPTSNEVPKLATGKGSLTPAQLNDIRSHGVFRVPSREFPGQFHVWK
                     VNSWLQEGIEHIEVRFYKNRDTGL"
BASE COUNT          503 a          517 c          475 g          514 t
ORIGIN      
        1 cgctaaagac ttgatcggat cggacgacat taagagcaac acggtagaat tcgttcttac
       61 caagacttac tttgagaacc ttggcgtgtt gatgcaagaa tatttgacta caaccagcgt
      121 tggcgatctc attcgcaaag cgcgcggcca cgacgttcaa aagaacgttg gaaccgacac
      181 agttcgcccg atgattgctc cagaagcaac tcgtggtgaa acgactacga agcttggacg
      241 caatttatct tgattataat gcttccgcac cttttccgac ggagctgaac gaagagttca
      301 gctccgttct tctttctggt tacggcaatc cttccagtct ccactttttt ggacgaaaat
      361 ctcgacgcct catcaatgaa gcaactgagt ttgttgcaaa gttcgttaac gccgatcccg
      421 cgttttgtta ttggacttct ggcgcatctg aggccaatac ctgggttttg cattcagcgg
      481 tacttcgtgc caaagccgaa ggccgttcac cacaattact tgtctccgcc attgagcacg
      541 aatcgacatt gttagcggcc aagcacctgg caagtcagtt taaagatgta cgcgtcgatc
      601 tattgccagt acttcgcaat ggacagattg atctagaagc tctcgctcaa atgctcacgc
      661 aagcgcgtta tgatttagtg agcgtcttgc acgctaataa cgaaacaggc gtagttcaac
      721 cagtcgatca gctaaccgag atttgcgctc gtgcgaatat tcccgtccat attgattgcg
      781 ttcaaacatt ggcgaaaatt cctttcacga ttgcagatcg cggcattcag tttttgactt
      841 tttctggtca caaaattgga gccccaaagg gagtaggttt gctcgcggtt cgcggggagg
      901 gacgtctact gtatcccttg attcatggta aacagcaaaa aggcttgcgc ggcggaaccg
      961 aaaacgtaac gggcgttacc atgttcgctc gcacgctaag cgcttatcaa aacggaaaaa
     1021 ttcgttcgtt cgaacactgc cagaccctgc atgatcgttt cgaaaaacaa ttaacggaaa
     1081 ctattccggg aacaatcatt cacggcctag gtgccgcgag attgccaaac accacttacg
     1141 tcggctttga agggctcgat ggcgacagtt tactcatggg tcttgatctc gagggcgttt
     1201 gcgcttccag cggatcggcc tgtagctcgg gaagtctcga ccccagtcac gtgctaagtg
     1261 ccatgggttg tacacccgaa ctcgccagat cttccgtgag atttagttcc ggtcccaata
     1321 cgacttggtc cgaatttgaa actcttttaa acgtgctctc cgaactcgtc tcgcgacttc
     1381 gcacctgaag ttgccaaccc cactattgga tgctaggcgt cacaccacga ttctaaaagt
     1441 tcaaaagggg tgtatatgtc tttgctacga accttccgtt tgttcagcct tggtgttttg
     1501 cttcttggcc tacagtttct ctcggtagca agtgccgctg attcagaggc gaacgaacca
     1561 aacctcgaaa tccctcaaga cacgaccccg gtgagcgaac gcactccagt acgaatttgg
     1621 atcgaggcag acttactccg aagcatgacc ccagaacaac gtgaggatgt tcaaaagatc
     1681 attgcaattg cggaagagcg actttttact tggaaaaact ttggttttcc cgagatgccc
     1741 gaatcaatgt tcagcccgga tgaaccggcg atcgttgaaa tcgcgtcttt attcatgaca
     1801 cctacttcaa atgaagttcc aaaattagct acgggcaaag ggtccctcac gcccgcgcag
     1861 ctcaatgata ttcgttccca tggtgtcttt cgcgtaccca gtcgggagtt tcccggacag
     1921 ttccacgtct ggaaagtgaa cagctggctt caagaaggta tcgagcacat cgaggttcga
     1981 ttctacaaaa accgcgacac cggtttgtt
//
LOCUS       DAOMXO010000464         2000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_33185_length_2000_cov_4.122879, whole genome shotgun sequence.
ACCESSION   DAOMXO010000464 DAOMXO010000000
VERSION     DAOMXO010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33185_length_2000_cov_4.122879"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1070
                     /locus_tag="PLH57_09175"
     CDS             144..1070
                     /locus_tag="PLH57_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRK02824.1"
                     /translation="MNFALLMDSFTVVVAMAFVFVSVYLTARAFFHSEETRSARELLG
                     EQNQTKKSRDAIVKYSRLVFSRYFVPVVANLKIEEVRKKTRRKLISAGMTEEFTADEF
                     LAFKAFMIFGAPAATGIFIYIMEIPLPWYGYFIVSLVGFFYPDMMVKGAIEGRQKEVR
                     KALPFVVDLLALSTEAGLDFMQAVGKVVEKATPSPLIDELSQVMREIKVGASRSEALK
                     EMGVRLNMREVSSFVAILVSADQMGASIGKILRQQSEAIRNERFVNAESQGAKAAQKV
                     MGPLFVFIIPAIIIMIFGPVALQMMKGGGMGP"
     gene            1067..1417
                     /locus_tag="PLH57_09180"
     CDS             1067..1417
                     /locus_tag="PLH57_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRK02825.1"
                     /translation="MNTQFKSITRKSDGYCVVRELENATSVIARMKGLLGRNNLPIGR
                     GLLIEPCNSVHTWFMRFSIDVVYLSKEMRVLKINRDMKPWRVDFPVRHARSVLELASG
                     AAYVLAEGDELCIN"
     gene            1405..1782
                     /locus_tag="PLH57_09185"
     CDS             1405..1782
                     /locus_tag="PLH57_09185"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRK02826.1"
                     /translation="MYKLTVVGGPTRGSTYSVNDGNVSIGRVSGNDIILESNRVSKRH
                     CVLVVDNTGVTVKDAGSSNGTFVNGVLAKIKKLVPGDRISVGEYILELTRIEIAAPPP
                     KVESNFSNVVPFPTPVVDPSAAD"
     gene            complement(1730..>2000)
                     /locus_tag="PLH57_09190"
     CDS             complement(1730..>2000)
                     /locus_tag="PLH57_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02827.1"
                     /translation="TQTRSQLITAREGLNDQARDQEVLAGERNRLSALNQQLEEQLGS
                     LRYLWNSKSDELEKTRASLTAMERLNADLSRQLTDLRRASETEQR"
BASE COUNT          514 a          437 c          522 g          527 t
ORIGIN      
        1 ctttttgccg actctagcac caaagaacaa aaatttcgaa gtggacgcgg cgttttcgcc
       61 ccagtcgttc gtacgaaccg caagattaag ttaatgattt tgctcatatt ccgaatagta
      121 acacaggaga gaagggtaaa agcatgaact ttgctttgct catggattcg ttcacagtag
      181 tcgtggcaat ggcgtttgtc tttgtgtcgg tatacctaac ggcacgggca ttctttcatt
      241 ctgaagaaac gcgttcagcg cgcgaactct tgggcgaaca aaatcagaca aaaaagtcgc
      301 gcgacgccat cgtaaaatat agtcgattgg ttttctcgcg gtatttcgtg ccagtagtcg
      361 ccaacctcaa aatcgaagag gttaggaaga aaactcgccg taagctaatt tctgcgggaa
      421 tgaccgagga attcaccgcc gacgaatttt tggcattcaa agcgttcatg attttcggtg
      481 cacctgccgc aactggaatt tttatatata tcatggaaat tccactgcct tggtacggat
      541 acttcatcgt gtcgctcgta ggttttttct atccagacat gatggttaaa ggcgctatcg
      601 agggccgcca gaaagaagtg cgtaaggcgc tgccgtttgt agtcgatttg ttggccctct
      661 ctaccgaagc cggactagac ttcatgcagg cggtgggtaa ggtcgtagaa aaagctacgc
      721 ctagtcctct gatagacgaa ctttcgcaag taatgcgtga aataaaggtt ggggcctctc
      781 gatctgaggc gctcaaagaa atgggagtgc gtttgaatat gcgagaagtt tcgtcattcg
      841 tagcaattct tgtttcggcc gaccaaatgg gcgcatctat cggaaaaata ctacgtcaac
      901 agtctgaagc gatcagaaac gagcgatttg taaacgcgga aagccaaggt gccaaggccg
      961 ctcaaaaagt aatgggtcca ctattcgtat ttattattcc agcaatcatc attatgattt
     1021 tcggtccagt tgcgctgcag atgatgaaag gcggaggaat ggggccatga acacacaatt
     1081 caaatcgatc acgagaaagt ctgacgggta ttgcgtagta cgagagttag aaaacgcgac
     1141 cagtgtaata gcgcgaatga aaggcctatt ggggcgtaac aacttgccga ttggacgtgg
     1201 ccttctgatc gaaccatgca attcggtaca tacttggttc atgcggtttt ctatagacgt
     1261 cgtctatttg tcgaaagaaa tgcgagtttt aaagataaat cgcgacatga aaccttggcg
     1321 agtggatttt ccagttcggc acgcgcgtag tgtgttggag ctcgcgagcg gagccgctta
     1381 tgttttagca gagggggacg agttatgtat aaactaactg tcgtcggtgg tcccactcgc
     1441 ggctcgactt actccgtgaa tgacggcaac gtatcgatcg gtcgggtctc tggaaacgat
     1501 attatcttgg agtccaatcg ggtctcgaaa cggcattgtg ttttggtagt ggacaatacc
     1561 ggggtcacag taaaagacgc gggtagttcg aatggtacat tcgtaaacgg ggtattggcg
     1621 aaaattaaaa aactcgttcc gggtgaccgc attagcgtag gtgaatacat cttagaactc
     1681 acccgaattg aaatcgcagc gccgcctcct aaagtagaat caaatttttc taacgttgtt
     1741 ccgtttccga cgcccgtcgt agatccgtca gctgccgact aagatcggca ttcaatcgct
     1801 ccatggccgt caaagaggca cgagtttttt caagctcgtc tgatttgcta ttccagagat
     1861 atcgcaagct tccgagttgc tcttcaagtt gttgattgag cgcgctcagt cggtttcttt
     1921 cgccggccag gacctcttga tcgcgagctt gatcattcaa gccttcgcgg gccgtgatga
     1981 gttgtgagcg tgtttgcgta
//
LOCUS       DAOMXO010000465         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_33262_length_1997_cov_4.300721, whole genome shotgun sequence.
ACCESSION   DAOMXO010000465 DAOMXO010000000
VERSION     DAOMXO010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33262_length_1997_cov_4.300721"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /gene="gspN"
                     /locus_tag="PLH57_09195"
     CDS             <1..839
                     /gene="gspN"
                     /locus_tag="PLH57_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HRK02828.1"
                     /translation="FLIFKFPEGRIQNYLIAHARIVMQNEGLSFAAEKVSLSFIFGPA
                     VKFYNIEIRAIDDDSQILKIPFLKIRPKISSLIFKNKKLGISAELLGGELSGTVGAGA
                     SGSMLIDLDVDRINLALSTLVKRYTIVELKGEVDGKVEIDFDGVDPSKTEGIIALKLK
                     NIQIPDQIAYGFNLPTLNIKQCVIDIPIGQGKADFRLVECGKDVNKDDIIGNLSGQIT
                     FDRVINNSKLNLRTVFQLSDTVVKAFPLLDALLASAKSSDGKYLYRIGGSISGIDPVP
                     GG"
     gene            847..1875
                     /locus_tag="PLH57_09200"
     CDS             847..1875
                     /locus_tag="PLH57_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02829.1"
                     /translation="MFSRSKLRPILVVGDSWPTIEVDRDSSLYLLDQGTRNHRVAAWW
                     AEPKDLFVRANELWVNAFPVLFKDSAVVPFPKLEKKRKLIPVKKFSHLLWRCDPPVTL
                     ETHRMWALLDCDSESNVVNDPRAMAVWNEKFALLKYSKWAIPSWIINDEAALRRELTG
                     LRGKSLLLKPSAAAASYGVKLLSKSLKSNLAIYRNTVKKYGPVVVLQEVDHRIHQLGE
                     FRFFAIGGRVVDAVQKFPGSNDPVMNWYTTKINQPRLQMADSNTVEFRKAERRARQVA
                     KDLMRDGIFLASIDFIGERILEINVTSPGLLKWVDEVGRFTQKKPKRKTLAELFWGEL
                     FTSSNKRR"
BASE COUNT          552 a          412 c          485 g          548 t
ORIGIN      
        1 tattcctcat ttttaagttt ccggaaggtc gaattcagaa ctatttgatc gcccacgctc
       61 gaattgtgat gcagaacgag ggtctttcat ttgcggccga aaaagtttct cttagtttta
      121 tttttgggcc agctgtaaaa ttttataata tcgaaataag ggcaattgac gacgactctc
      181 agatactgaa aattccattc ttaaaaattc gacccaaaat tagttcactc atttttaaaa
      241 ataaaaaatt aggaattagt gccgagttgc tgggtggtga gttgagtggg accgtcggtg
      301 cgggcgcgag tgggtctatg ctgatcgatc tcgatgttga tcgaataaat ttggcgctct
      361 ctactctcgt taagaggtac accattgtag agttaaaggg cgaggtcgac ggtaaggtcg
      421 aaatcgattt cgacggtgtt gatccatcta aaactgaagg aataattgcg cttaaactca
      481 agaatataca aattcccgat caaattgcct acggatttaa tcttccgacg ttgaatatca
      541 aacaatgtgt tatcgacatt ccgatcggcc agggtaaggc ggattttcgg ttggtcgaat
      601 gcgggaaaga cgtaaataaa gacgacatta tcggaaattt atctggtcaa attacgtttg
      661 atcgagtcat caataattcc aaacttaatc ttagaacggt gtttcaatta tccgataccg
      721 tcgtaaaggc atttccgctc ctagacgcat tgctcgcaag cgctaagtcc tcggacggaa
      781 aatatctcta tcgcattggt gggagtattt cgggcatcga ccctgttccc ggaggttagc
      841 gaaccaatgt tttcgagatc gaaactacga cccattcttg ttgtcggcga ctcttggcct
      901 acgatcgagg tagatcgaga ttcttcttta tatttactag atcaggggac acgaaaccac
      961 cgcgtagcgg cttggtgggc tgaacccaaa gatctcttcg tgcgagctaa tgagttatgg
     1021 gtcaatgcct ttcctgtact tttcaaagac agcgctgttg tgccgttccc taaattagaa
     1081 aaaaaacgaa aactaattcc cgtaaaaaaa ttttcccatc tactttggcg gtgtgatccg
     1141 cccgtaacac tcgaaaccca tcgcatgtgg gctttgttag actgtgattc agaaagcaat
     1201 gtagtgaacg acccgcgagc catggcggtg tggaatgaga aatttgctct tctcaagtat
     1261 tcgaagtggg caattccgtc atggatcatc aacgacgaag ccgctctgcg acgggagcta
     1321 acaggtcttc ggggaaaatc actcttgttg aaaccaagcg ccgcagctgc aagttatggc
     1381 gtaaagcttc ttagtaaatc tttgaagtcg aacttggcaa tctatcgaaa cactgtgaaa
     1441 aaatacgggc cagtggtcgt acttcaagag gttgatcatc ggattcatca gctaggggag
     1501 tttcgttttt tcgctatcgg cggaagagta gtagatgccg ttcaaaaatt cccaggttcg
     1561 aacgaccctg taatgaactg gtacacaacg aaaattaatc aaccgcgctt gcagatggcc
     1621 gattccaaca cggtggaatt ccgaaaggcc gaacgtcgag cgcggcaggt cgccaaagat
     1681 ttaatgcgag acgggatatt cttggccagt atcgatttca tcggcgaacg cattttggaa
     1741 attaacgtta ctagcccggg cttattgaaa tgggtggacg aagtagggcg gtttactcaa
     1801 aaaaagccaa aacgaaaaac tctggccgaa ctcttttggg gcgaattgtt tactagttcg
     1861 aacaaaaggc gatgagggat tgagtttcga ggatcgcttc attgattaat ctgaagcgat
     1921 cttggtcgat tgcaatcttg attttctttg aagcagtgag ttcgcccgat tttcgcattt
     1981 cggcgtcgcg ggcgaaa
//
LOCUS       DAOMXO010000466         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_33334_length_1994_cov_4.425477, whole genome shotgun sequence.
ACCESSION   DAOMXO010000466 DAOMXO010000000
VERSION     DAOMXO010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33334_length_1994_cov_4.425477"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLH57_09205"
     CDS             complement(<1..192)
                     /locus_tag="PLH57_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HRK02830.1"
                     /translation="MKAPVKRVEKPWGYELIFAATKDYVGKILHIKKGHKLSLQYHRV
                     KEETIYISQGEMLFVFEEDG"
     gene            complement(189..824)
                     /locus_tag="PLH57_09210"
     CDS             complement(189..824)
                     /locus_tag="PLH57_09210"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRK02831.1"
                     /translation="MFNKKEIVIILLLFVLVALLSFTLGVRMGRSLNETKLYTETPQE
                     APLKEKAALPIEKAPAHGEDQSAHDNQGDSKSLLKKESATEKESAKAEDLADGELQAE
                     LTKSGVKTDRPVSMELPTEKKGEMAPAKSVAGRFTLQVGSHRTVSEAAEQVTSLKAKG
                     LDAFYIEAVLPGKGTWYRVGVGNFDSKEKAELYAARYKNELPPFIVQRMSE"
     gene            981..1220
                     /locus_tag="PLH57_09215"
     CDS             981..1220
                     /locus_tag="PLH57_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02832.1"
                     /translation="MNNNVNGTNCPKYLVRGQGLVEYIALTALVAVVCIGTVKVFGSK
                     VTRQLNSVSNKFDQTIRKGLNTKVANPDPFRDDQD"
     gene            1609..>1994
                     /locus_tag="PLH57_09220"
     CDS             1609..>1994
                     /locus_tag="PLH57_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02833.1"
                     /translation="MNLQRNLDRCLVNILKDRCATLATLSTQNARLVSLARAHRAAML
                     ATANPATAGIAQGTARALEAAAKGLVILQDIELWRARSAAVHLMTCGATLLDYAPLRI
                     RRKKFDTTTVPSFPKPLEWLNAKSAA"
BASE COUNT          484 a          512 c          450 g          548 t
ORIGIN      
        1 gccatcttct tcgaagacaa aaagcatttc accttgagaa atatagatcg tttcttcttt
       61 tactcggtga tactgcagcg aaagtttgtg gcctttttta atgtgtaaaa ttttaccgac
      121 gtaatctttg gtggctgcaa atatcagctc gtagccccaa ggtttttcta cacgtttaac
      181 aggcgccttc actccgacat cctctgtaca ataaatgggg gaagctcgtt tttgtagcga
      241 gcagcataca gctcggcttt ttccttgctg tcaaagttcc ctactccaac tcggtaccaa
      301 gtgcctttcc ccgggagcac cgcttcaata taaaacgcat cgagcccttt agcctttaag
      361 gaggtcacct gttctgcggc ttcagatacc gtgcggtgag acccaacctg caaagtaaaa
      421 cggccagcca ctgatttagc aggtgccatt tctcctttct tttcggttgg tagttccata
      481 ctaactggtc gatcagtttt tacgcccgac tttgtgagtt ccgcttgtag ttcgccatcg
      541 gccaaatcct ccgccttagc ggattccttc tcggtagcgg attccttctt tagtaaactt
      601 ttcgaatcac cttggttgtc atgagcgctc tgatcttccc cgtgggctgg ggctttttct
      661 atcgggagag ccgctttttc ctttaatggc gcctcttgag gtgtttctgt atagagtttt
      721 gtttcattca gcgaacgtcc cattcgaacg cccaaagtga acgacaaaag cgccactaaa
      781 acaaagagta agagaatgat cacaatctct tttttattaa acacaaccaa ttcgtgtcgt
      841 ggttccgaat gcctggtatt ttgcgatgtg gggtttgcca tagtgtctga gtctgacttt
      901 aagctttaga actaagcctc gtcaagccaa tggtccgaac cttgctacag gttcatgcgt
      961 tatattttta aggagtaaac atgaacaaca atgtaaacgg gaccaattgc cccaaatatc
     1021 tagttcgcgg ccagggcttg gtggaatata tcgcgctcac cgcattggtt gccgtggtct
     1081 gtatcggaac cgtcaaagta ttcggatcaa aagtaactcg gcaactaaac agtgtttcga
     1141 acaaatttga tcaaactatt cgcaagggtt tgaacacgaa ggtagctaac ccggacccat
     1201 tccgagatga ccaagattaa tgaaatccct tcgccacatc aacaggcgcg gtcaggcgct
     1261 actggaatgg gttattgttt tagtcgggac aaccctcatt ctcagcacta ccctgttttt
     1321 caccaaacaa gcgttccacg ctacgcttgc ccaaacctgg acgctatttg cggctcgcgc
     1381 caaactttcc cgagtagagc ttggcacacc tcgaggcgtt cggattcatt tatacagaca
     1441 ccgaaaccaa gtagtcgcta cctcagggtt tggagccgca aaaatccaag cgaaagtgtc
     1501 ggtgccagag aaaaaggatt cgctagcgtc ttctttttgg tctgttttct accggtaatt
     1561 ctcgtatggg cactaattct aggaattcga aaaaatgctc ttcaagaaat gaatcttcag
     1621 cgaaacctcg atcgatgttt ggtgaacata ctgaaagatc ggtgtgcgac tcttgccacg
     1681 ctatcgactc agaacgcccg tctcgtatct ttggctcgag cccaccgcgc tgcaatgcta
     1741 gctactgcaa atccagccac tgcgggcatc gctcaaggaa ccgctcgggc gctcgaggcc
     1801 gcggcaaagg gattagtgat tttgcaggac atcgaacttt ggcgcgctcg cagtgcggcg
     1861 gtgcatctca tgacctgtgg tgctactttg ctggactatg cacccctgcg aattcgccgc
     1921 aaaaaattcg acacgactac ggttcccagt ttcccaaaac cgctcgaatg gctcaatgcc
     1981 aaatcagcag cttt
//
LOCUS       DAOMXO010000467         1982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_33643_length_1982_cov_5.014530, whole genome shotgun sequence.
ACCESSION   DAOMXO010000467 DAOMXO010000000
VERSION     DAOMXO010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33643_length_1982_cov_5.014530"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..>1982
                     /locus_tag="PLH57_09225"
     CDS             54..>1982
                     /locus_tag="PLH57_09225"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRK02834.1"
                     /translation="MTVGKIFPYRLMIMQFRAVFFRGLVLASFLVFLSVLSVVASANE
                     GVATVGDVLVERDRPRFNDQIAHDPSKIRLERPNLDAELRETVGKLPLLTSGSRSGFG
                     SASSVITATGLSSAATAVSIDGVPIVDPNGVGFNFSLVPSALVESVEYWLPHHQALDP
                     ASQTLASTSGRMNLRTKASRRLRNRFGSTFTVGSYQSILGSAVYERVTNSDSAAFAIT
                     GHNTKGDYEYKNDRNGSRERRANNSSAGGGALARWSRVFDRDGASGVDVFGLASGNAR
                     TNPGSLEAPGRDRQEDLFSVIGASARNPEFFNKSSGASVALAGSLSKVAIKNPPLYEN
                     RTFGGFAKVAVEKRLRGATGKGADLHFAVDDHVDYVRADSGRLNRNILGLAAAVPVHL
                     TSEITFAPWVRQELANRMPSTRDAIAAMTWSLGRDNHFTASYGYLHAYPTITQIAGEH
                     TPFGEVLPNNSLKLERSGVWTAMFTHESERVQFLSLVQHARIRNRISFESVFNGTSFR
                     SQYVNAADAFVTAWSNDLQWNVVSFIGLRAAGTVSRSFDFQTRRDLAYKPRVIGLGSV
                     VCDLGAFARALTLLPKARSGFLFLSLEESVWGKRWTGRTGVDTISPIAQTHVRLALQS
                     GPLEMAVRAANIFEESGFD"
BASE COUNT          397 a          497 c          581 g          507 t
ORIGIN      
        1 gtgctcaccc aaagaggtta acacgaatta ttttgaatca cttcgaagtg atcttgactg
       61 tcggcaaaat attcccgtat aggctgatga tcatgcagtt cagggccgtt ttcttccgag
      121 gcttggttct agcctctttt ttggtttttc tctcagtctt gagtgtggtt gcgagtgcta
      181 acgaaggcgt cgccacagtg ggagacgtgt tggtcgagcg cgatcgacct cggtttaatg
      241 atcagattgc tcacgatcct tcgaaaattc gattggagcg gccaaatcta gatgccgagt
      301 tgcgtgagac ggttggtaag cttccgctgc tgacaagtgg gtcgcgctct ggttttggga
      361 gtgcgagttc ggtgattacg gcgaccggac tttcgtcggc ggccactgcg gtgagtatcg
      421 acggtgtgcc gatcgtagac cctaacggcg taggttttaa tttttctctt gttcccagtg
      481 ccttagttga gagtgtcgag tactggttgc cgcaccatca ggcgctcgat cctgcgtcgc
      541 agacgctggc ctcgacgagt gggcgcatga atcttcgtac caaggcttcg cgccggcttc
      601 gcaatcgctt tggttccacg tttaccgtcg gttcgtatca gagcattctt ggatctgctg
      661 tttacgagcg agtgacgaat tccgattccg cggccttcgc cataaccggg cacaacacca
      721 agggcgatta cgagtataaa aatgatcgca acgggtcccg cgaacgacgc gccaacaata
      781 gttcggccgg tggtggcgca ctcgcccgtt ggtcgcgggt ttttgaccgc gacggcgcca
      841 gtggcgttga tgtgtttggt ctggcctcgg gcaatgcacg cactaatccc ggttcacttg
      901 aggctcccgg gcgcgatcgc caggaagatt tgttttcggt cattggcgct tccgcgcgta
      961 atcccgaatt ttttaataaa tcgagcggcg cgagtgttgc gttggcgggt tccctatcta
     1021 aagtagccat caagaaccca ccgctttatg aaaaccgcac ttttgggggc ttcgctaaag
     1081 tggcggttga aaaacgattg cggggagcca ctggaaaggg cgccgatttg cattttgccg
     1141 tcgacgacca cgtcgactat gttcgtgccg acagcgggcg tttaaatcga aatatcttgg
     1201 ggctagcagc tgcggtacca gtccatttga cttcggaaat cacgtttgcc ccttgggtgc
     1261 ggcaagagct tgcgaatcga atgccgtcga cccgcgatgc aattgccgcg atgacctgga
     1321 gtctggggcg cgataatcac ttcactgcat cgtacgggta tttgcacgcc tatccgacaa
     1381 tcacccaaat tgcgggcgaa catacgccgt ttggtgaagt cttgccgaat aactctttaa
     1441 agctagagcg cagcggtgtg tggaccgcca tgttcactca cgagtcagag cgcgtgcagt
     1501 ttctttcgct cgtgcagcac gcccgcattc gcaatcgaat tagttttgaa agtgtcttta
     1561 acggcacgag ttttcgatcg cagtacgtca atgcggccga cgcttttgtg accgcctggt
     1621 caaatgacct acagtggaac gtcgtctctt ttattggact gcgggccgcc ggaacggtga
     1681 gtcgctcatt tgattttcaa accaggcgag atcttgccta taaaccccgg gtaattggac
     1741 tagggtcggt agtgtgcgat ctgggagcgt tcgcgcgggc gctaactttg ttgccgaaag
     1801 ctcgaagcgg gtttctgttt ttgtcgctcg aagagtcggt ctggggaaaa cgctggaccg
     1861 gacgaacggg agtggacacg attagtccga ttgctcaaac tcacgtgaga ttagcattgc
     1921 agtccgggcc cctagagatg gcggtacggg ccgccaatat ttttgaggaa agcggattcg
     1981 ac
//
LOCUS       DAOMXO010000468         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_33959_length_1970_cov_4.137337, whole genome shotgun sequence.
ACCESSION   DAOMXO010000468 DAOMXO010000000
VERSION     DAOMXO010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33959_length_1970_cov_4.137337"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLH57_09230"
     CDS             complement(<1..405)
                     /locus_tag="PLH57_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02835.1"
                     /translation="MKALAALAVHLKLFAKYLLFSVVVIGVFQFPEILAKFWSEKIPF
                     QTIKLNPREIKTAPLALEAGHYFVSYGIVRGACDALLNDKVADSNMSEGNGIRSKIML
                     GHEFIVIKDTPVTLALRCDYQAGFTTSLANEPL"
     gene            complement(402..>1970)
                     /locus_tag="PLH57_09235"
     CDS             complement(402..>1970)
                     /locus_tag="PLH57_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02836.1"
                     /translation="KLMSGLRLIEFSADDYRIADVLSRPHLEKLNDSQKISAALSLYF
                     QWREHRRCFISVADNKEFVECDLAVDQDLVTPIEIVALVDKKLVDAVPDRARVRFEIF
                     RTCYEFSSPITADLSDSTKSAFAFDIPSEVYVHKARTSPRYAVPLDNPLRRSKWIDPN
                     GSEQEVEVIDLSVKVLHVAANNRILSDFGLIELDGHRFNAQLIRKSSVGHAVRIKFEH
                     SGEFGKFFDRFIKVAYPELAPREHFKNENIYELYRDTGYLSSFNLGECEEEEEKRREL
                     LVKYWNESQNETHQTKVDYVLKDHADKACGASGLARIFKKDGKAIWAFHQLCVRKESA
                     SLTGTGTLYNWRVEYLEGLGAPVEAVVWYKSSSKWLERIFTKLAVANARDTQLEAVHL
                     IRLPRQKSTEALSDSLEVDGPRKFMNMPGVLVGSGPEFLNASRLFEMVWVTDQTTNQE
                     ELTHAREVYHRLAGDNEYDFYSISIPAKSDIKLEHETRPTDRCFRLNPAAFSDLISSV
                     EHSIAVTKKKQAKT"
BASE COUNT          495 a          492 c          451 g          532 t
ORIGIN      
        1 gagcggttca ttggcgagcg aagtcgtgaa tccagcttgg tagtcgcagc gtagtgccaa
       61 cgtcaccggt gtgtctttga tgacgatgaa ttcgtgaccc aacatgattt tggatcgaat
      121 gccgttacct tcgctcatgt tgctatctgc cactttatca ttcaacaaag cgtcgcaagc
      181 ccctctcact ataccgtaac tcacgaagta gtggcccgcc tcaagcgcta gtggtgctgt
      241 ttttatctcg cgcgggttca gcttaatggt ttgaaacggt attttttcgg accagaactt
      301 ggccaaaatt tcgggaaact gaaaaactcc gatcaccacc accgaaaata gcagatactt
      361 cgcaaatagc tttaaatgaa cagcaagagc agctagtgct ttcatgtctt agcctgcttt
      421 ttcttggtga ccgctattga atgctcaacc gaactaatca aatccgaaaa tgccgcaggg
      481 ttcaaccgaa agcagcggtc ggtggggcga gtttcatgtt ctagtttaat gtctgatttt
      541 gcgggaatcg aaatgctgta aaaatcgtat tcgttgtcgc ccgcaagccg gtggtaaacc
      601 tcacgggcgt gggttagctc ttcttgattc gtcgtctgat ctgtcaccca aaccatttca
      661 aaaagccgac tggcgttcaa gaactcgggc cctgaaccga cgagcacgcc aggcatattc
      721 atgaactttc gcgggccatc gacttcgagc gagtccgata gtgcctcagt gctcttttga
      781 cggggaagcc ttattaagtg caccgcctca agttgggtat cgcgggcatt cgctaccgct
      841 aatttggtga aaatacgctc gagccatttg ctggacgatt tgtaccaaac caccgcttcg
      901 accggtgcac ccaaaccttc gagatactcg actcgccagt tatacagggt accagtcccc
      961 gtaagagaag cggactcttt tctcacgcaa agctgatgaa atgcccaaat ggctttcccg
     1021 tcttttttga atattcgagc aaggccagac gcgccacaag ctttgtctgc atggtcttta
     1081 aggacgtagt caactttggt ttggtgcgtt tcgttttgag actcgttcca atatttgacg
     1141 agtaattctc gacgtttttc ctcctcttct tcgcactcgc caagattaaa tgaacttaag
     1201 taacccgtgt cacgatacaa ttcgtagata ttttcattct tgaaatgttc acgcggggct
     1261 agctcgggat aggctacttt tataaaacga tcgaaaaatt ttccaaattc tcctgaatgc
     1321 tcaaatttta ttcgcactgc atgaccaaca ctcgacttgc gtattagctg cgcgttgaag
     1381 cgatgaccgt ctaactcaat taggccaaaa tcgcttagaa ttcgattatt agcagcgacg
     1441 tgtaacacct tcacgctaag atcgatgact tccacttctt gttccgaccc gttcggatcg
     1501 atccactttg aacggcgtag cggattgtcg agcggcaccg catagcgcgg actggtgcgc
     1561 gccttgtgga cgtaaacttc actaggaatg tcgaacgcaa acgcggattt cgtgctgtcc
     1621 gataaatcag cggtaatggg actggaaaat tcgtaacagg ttctgaaaat ctcaaaacgt
     1681 actcgcgcgc gatccggcac cgcatcaacc agttttttat cgactaatgc gacgatctcg
     1741 ataggcgtaa cgagatcctg atccaccgct aaatcacatt caacgaactc tttattgtct
     1801 gcgacactta taaaacaacg tcgatgttcg cgccattgga agtacagcga caaagctgct
     1861 gagattttct gtgaatcatt caatttttct aaatgcgggc gactcaggac gtcggcaatt
     1921 cggtaatcgt cagctgaaaa ttcgattaac ctaaggccgc tcatgagctt
//
LOCUS       DAOMXO010000469         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_33991_length_1969_cov_4.033438, whole genome shotgun sequence.
ACCESSION   DAOMXO010000469 DAOMXO010000000
VERSION     DAOMXO010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33991_length_1969_cov_4.033438"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLH57_09240"
     CDS             complement(<1..281)
                     /locus_tag="PLH57_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRK02837.1"
                     /translation="MGKKLFVGNLPFSATDESLAQIFAQVGTVESAKVITDKMSGRSK
                     GFGFVEMADDAQAADAVARFNGIELEGRPLTVAEAKPQEPRQDRGGRGG"
     gene            complement(601..>1969)
                     /locus_tag="PLH57_09245"
     CDS             complement(601..>1969)
                     /locus_tag="PLH57_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02838.1"
                     /translation="KVNDLLSAMYMEPHSPGGLHFVTFETLGENPTPVPFKDLPTIDS
                     SKLVMKTHSLSLSYLSYPFAHTPTLPLVAGSLRLENRTVGVPDLVPSDRGSRPLVLGQ
                     VTESLALDVPIGGTNHLISVSEVRYESGIALLGDTLEARGRFRDAVLHEARTDLRESL
                     KRNRGHEPVNRAALNRMFPGLEVSAKSLMASLRPFLIAEDASEVDATLKRLEEFFYRL
                     AAQSPTLVETSERDFLAGQKIPSTLPRSVPNPETATVRLTRLSEFFFESAQSSHLARE
                     RGEIENAKIAATNAFRVLLEMHVVIGKNLGSPGTKRTLHALGDVGIMIASMIASWNTD
                     GGAHAGLLALSFAAMADLFRTTWVRDNVLRSPRFLFPGIRGSVERNSARDLLSQGRFE
                     NLLQVGLRSPVIINPQFQDWHRAASELAENGKLVSAREMLDVLKLASKLEADALVSCA
                     TLLEN"
BASE COUNT          473 a          492 c          539 g          465 t
ORIGIN      
        1 aaaccaccgc ggccgccacg atcttggcgg ggctcctgcg gtttagcttc agctactgtg
       61 agtggtcggc cttcgagttc aatgccattg aagcgcgcaa cggcatcggc tgcttgcgca
      121 tcgtcggcca tttctacgaa tccaaagcct ttactgcgcc cggacatttt gtcagtgatc
      181 accttcgcgg actcaacggt gccgacttgt gcgaaaattt gcgcaagcga ctcgtcagtt
      241 gccgaaaatg gaaggttgcc gacaaatagt ttcttaccca tgaggtctcc tctttctaga
      301 accagaacta tcgaaatccg gacaagaggt ctggaccatt gggaagacgt cttgggcggg
      361 tctgatagtg gacattaaca cccgttcgag gactattcct ttaaacgggg taccacctca
      421 cagacgtgtg acgcagtacg tcttcaatat tatcatgctt cgctacaaag gccaaaaggg
      481 aagtgttaag gacgataaac tatcgagaat aatcggtttg tccgacgagt tcggcagtcc
      541 atcaaaggga actccaatgt catttgcgat cacccggcag aggtgagtct gcgtgcgctt
      601 ttagttctca agtagcgtcg cgcaagaaac caatgcgtcg gcctcgagtt tggacgcaag
      661 ttttagaacg tcgagcatct ctcgcgcact tacgagtttg ccgttttctg cgagttcaga
      721 agcagctcga tgccaatcct gaaactgggg gtttattatt acagggctcc ggagccctac
      781 ctgcagtagg ttttcgaaac gtccttgact gagcaggtcc cttgcggaat tgcgttcgac
      841 gcttccgcga atgccgggaa ataaaaatct tggtgagcga agtacgttgt cgcgcaccca
      901 agtcgttcta aataaatcgg ccattgcggc gaaactcagc gccaagagac cggcatgggc
      961 cccgccgtcg gtgttccacg acgcaatcat tgatgcgatc atgatgccaa catcgcccag
     1021 ggcatggaga gttcgcttgg tgcccggaga accgagattt tttccgatca caacgtgcat
     1081 ttcgagtaac actcgaaatg cgttagtggc cgcaatttta gcattttcga tttcgccgcg
     1141 ctcccgtgct aagtgcgacg attgagcaga ctcgaaaaaa aattcagaca atcgcgtgag
     1201 ccttacggtc gcggtttctg ggttcggtac agatctcggc aaggttgagg ggattttctg
     1261 gccggccagg aaatcacgct ctgacgtttc aacgagtgtc ggagattggg ccgcaagccg
     1321 ataaaaaaac tcttccaatc tctttagagt ggcgtcgact tccgatgcat cttcggcgat
     1381 taaaaaaggc cgtaaactgg ccatcaagga ttttgcgcta acctcgagtc ctggaaacat
     1441 gcggttgagg gcggctcgat tgactggttc gtgacctcgg ttgcgtttta aactttctct
     1501 tagatcggtt cgagcttcgt gaagaactgc gtcccgaaaa cgaccgcgcg cctctagggt
     1561 gtcgcctaaa agagcgattc ccgactcata acgaacttcc gaaacggaaa tcaggtgatt
     1621 ggttccaccg attggaacat ctagtgcgag gctctcggtt acctggccaa gcacaagcgg
     1681 gcgagagccg cggtctgaag gaactaaatc gggtacgccc acggttcggt tttcaagccg
     1741 tagtgaaccc gcaaccagcg gcagtgtggg tgtgtgagca aaggggtaac tcaaataaga
     1801 aagcgaaaga gagtgggtct tcatgacgag cttcgaagaa tcgatggttg gtaaatcctt
     1861 aaacggcacg ggtgtggggt tttccccaag agtttcaaat gtgacgaagt gcagtccacc
     1921 cggggagtgg ggctccatgt acatcgcgct caaaagatcg ttaaccttt
//
LOCUS       DAOMXO010000470         1962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_34146_length_1962_cov_4.545359, whole genome shotgun sequence.
ACCESSION   DAOMXO010000470 DAOMXO010000000
VERSION     DAOMXO010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34146_length_1962_cov_4.545359"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..749
                     /gene="queG"
                     /locus_tag="PLH57_09250"
     CDS             60..749
                     /gene="queG"
                     /locus_tag="PLH57_09250"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRK02839.1"
                     /translation="MSKYAHGPDYHEIMEKKLNELIEKISDHLGGAHRLFVDRHPVMD
                     RTWATLGGLGWIGKNTCVINRKAGSFFFIGGFFSTLKINADRPATDHCGRCRLCIDAC
                     PTHAILEPKQTGLNRHVIQSDLCISYQTIENRGEIPSAIQNKMGSWVVGCDICQQVCP
                     WNRQRGETMQPRQTWFKSQHSLASLTYEEGESLTASDWKSRVKGSAISRLKYQQFIRN
                     LKIAKKNSSSE"
     gene            complement(736..1938)
                     /locus_tag="PLH57_09255"
     CDS             complement(736..1938)
                     /locus_tag="PLH57_09255"
                     /inference="COORDINATES: protein motif:HMM:NF037020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02840.1"
                     /translation="MAKIDIGEDKNEIVPDKHPLSKSLSIFTDDLPKTQEDAVAEVRK
                     NIWTHWINSEPLLSAHIEDDGRSSIFFINNDEIKNKIILLDICDFTLLPCRRSSRYVK
                     EWYRRYSSAGLVVIGIHTPFYEFAKDRRIVEALLYAMDIQYPVVMDNDYLYWKAFDNR
                     FWPRRVLIDSKGKTHADFVGEGAYPEFEKKMQDLLRALSPGLPCPPILKPMRDVDDET
                     ASAAQTTSEIFLGTKYKQRLGNKAVPLRPNEEFLFTDETSDSLIPDVPYFKGAWSLHS
                     DHMEVYVPVDPKRAHKGEHSVTIQFTGTDVFLVAKTKSKILGDPTQSVKLNFLINGKP
                     LAEENFGEDISYGDTYRPQLIVRDPRLFHAVSKLDYGTHRFTVIVDPEGPDTAEIYAF
                     FFDSLTQS"
BASE COUNT          520 a          426 c          472 g          544 t
ORIGIN      
        1 cagtggctta agggtgttgt cgtctttttc gtaatcggcg gcactaccag aagccactga
       61 tttcgaaata cgctcacgga cccgattacc acgagataat ggaaaaaaaa ctcaatgagt
      121 taattgaaaa aatttctgac cacctgggag gggcacatcg attgtttgtt gaccggcacc
      181 cggtgatgga tcgcacttgg gcaactctcg gtggactcgg atggatagga aaaaatactt
      241 gtgtgataaa ccggaaggcg ggttcgtttt tttttattgg cggctttttt tccacgttaa
      301 aaataaatgc cgatcgtcca gcgacagacc attgtggtcg ttgtcggttg tgcattgatg
      361 cttgcccaac tcatgcgatt cttgagccaa aacaaacagg actcaatcga cacgtgattc
      421 agagcgattt atgtatttcc taccaaacca ttgagaacag gggcgagatt ccctctgcga
      481 ttcaaaacaa aatggggtcc tgggtggtgg gttgcgatat ctgtcagcaa gtgtgtcctt
      541 ggaatcgaca aaggggcgaa acaatgcaac cacgtcaaac ctggttcaaa agccaacact
      601 cgcttgcgtc attgacctac gaggagggag aatcgttgac cgcatccgat tggaagtcgc
      661 gagtgaaggg cagtgcgatt tctcgactta agtatcagca attcattcgt aacctgaaaa
      721 ttgcgaagaa gaactctagc tctgagtgag cgaatcgaag aaaaaggcgt aaatctcagc
      781 cgtgtccgga ccttcggggt ccacaatcac tgtaaaccga tgggtcccgt agtccaattt
      841 cgaaaccgca tgaaagagtc tgggatcccg cacgataagt tggggtcggt aggtgtcgcc
      901 gtaggaaata tcttcaccga aattttcttc ggctaaaggt ttaccgttga tgagaaaatt
      961 gagtttcacc gattgagtag gatcgcccaa gatttttgat ttagtcttgg ccactaaaaa
     1021 tacgtcagtt ccggtaaact gaatggttac gctgtgttca cctttgtggg cccgcttcgg
     1081 atccaccgga acataaactt ccatatggtc cgagtgaagt gaccaggcac ccttaaagta
     1141 cggaacatct ggaatcaatg agtccgaagt ttcatcggta aacagaaact cctcgttggg
     1201 ccgcaaaggc acggctttat taccaagtcg ctgtttgtat ttggtgccaa gaaatatttc
     1261 tgaagtggtt tgcgcggcac tggccgtttc gtcatccaca tctcgcatgg gttttaaaat
     1321 tggcgggcag ggaagtcctg gtgagagggc ccgcaaaaga tcctgcattt ttttttcgaa
     1381 ttcaggatat gcgccctctc ctacgaaatc ggcgtgtgtt tttcccttcg aatcgataag
     1441 cacccttcta ggccaaaatc tattatcgaa tgctttccaa taaaggtaat cgttgtccat
     1501 caccacgggg tactgaatat ccatagcgta caaaagcgct tctacaattc ggcggtcttt
     1561 tgcaaactca taaaacgggg tgtgaattcc gataaccact aaaccggccg aggaatatcg
     1621 gcgataccac tcttttacgt atctcgacga tcttcggcac ggaagcaatg tgaagtcaca
     1681 aatatcaaga agaatgattt tattttttat ttcgtcgtta ttgataaaaa atatagaact
     1741 tcgaccgtcg tcttcgatgt gagcacttag taacggttcg ctatttatcc aatgtgtcca
     1801 aatatttttt cgaacttcgg cgacggcgtc ttcttgggtc tttggtaaat cgtcggtgaa
     1861 aatcgagaga gattttgaaa gtgggtgttt atcgggaaca atttcgtttt tgtcttcgcc
     1921 tatatcaatt tttgccattg gatttcgcgt cttctctcaa ta
//
LOCUS       DAOMXO010000471         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_34179_length_1961_cov_3.986359, whole genome shotgun sequence.
ACCESSION   DAOMXO010000471 DAOMXO010000000
VERSION     DAOMXO010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34179_length_1961_cov_3.986359"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..958)
                     /gene="flgG"
                     /locus_tag="PLH57_09260"
     CDS             complement(176..958)
                     /gene="flgG"
                     /locus_tag="PLH57_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRK02841.1"
                     /translation="MIRSLSTAATGMQAQEAKLDQISNDLANMNTTSYKRGRTEFHDL
                     MYQTIKDPGGAPGVNQAPVGVQVGSGSKVAAQYSIQEQGPVKITNGLFDLMINGEGFF
                     ACQLPNGQIAYTRDGSFKMDAQGRLVTSSGYPMIPPIQIPPGTAGVTITGNGQVRVMT
                     AEGQDAEIGQIQTVNFLNPGAMKKIGENLVTPTASSGPPVQGTPGENTLGTIQQGAVE
                     ASNVKPTESIMDMISAQRTYESNARVMTVGDQMWSTLNNVMK"
     gene            complement(955..1923)
                     /locus_tag="PLH57_09265"
     CDS             complement(955..1923)
                     /locus_tag="PLH57_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HRK02842.1"
                     /translation="MGSPTGLWTSVSGSMAQSQAIDIAANNLANANTAGFKKDEPTFK
                     EYLTIHERPPSPDVDIPRTIFRDSDFYHHDGREHAMVNVDAVVTDHSQGTFRQTNGPL
                     DLAIEGPGYFAVQTPNGLAFTRAGDFKIAGNGQLMTNDGYRVLSLAGAAAEAGGETAD
                     AQAEVGRAPAAASPFSGALPPWGGGEQNAPGAVPQDQAQPGGLQPINLAEAMSSGSKL
                     RITDDGKIFVGNEEIAQIAMAEFSNPSQLLKKGAAIFTNPSPTNVASVARGSRIHQGF
                     IEGSNVNAVSEIMNVIKANRLFESNMKAIKAYNEMAVKEANEVGKL"
BASE COUNT          434 a          506 c          467 g          554 t
ORIGIN      
        1 tgaatccatg agtcttccac caaatcctgc aaacacaccg actgagagct gaccattgct
       61 gcgtttttta gacgtatttc tctttcgatc gccaggtcag gagtcgcaac aagtcccgcc
      121 atcgatgtgc tggacacgaa cagtaagaga gtaaagtact ttgtcatatc acccactatt
      181 tcattacgtt attgagagtg gaccacattt ggtcgccgac cgtcataacc cgcgcgttag
      241 attcgtatgt gcgttgtgcg gaaatcatgt ccattataga ttcagtcggt ttgacatttg
      301 acgcctccac ggcgccctgc tgaatggtgc ccaaagtatt ttccccggga gtgccctgca
      361 ctggtgggcc acttgaggcc gtcggagtaa ctaggttttc tccaattttt ttcatagcgc
      421 ccgggttgag aaaattgaca gtctgaattt gtccgatctc ggcatcctga ccttcggccg
      481 tcatgactct tacctgacca ttgccggtta tggttacgcc ggcggttcct gggggaatct
      541 gtattggtgg aatcatcgga taaccagaag acgtgaccaa acgaccttgt gcatccattt
      601 taaaagatcc atctcgagtg tacgcgatct gcccgtttgg caattggcac gcaaaaaaac
      661 cctcgccgtt gatcattaaa tcgaaaagac cattcgtgat tttcactggt ccttgttcct
      721 gaatggaata ctgtgctgct acttttgatc ccgatcctac ttgtacaccc acaggcgctt
      781 gatttacgcc aggcgcccca ccaggatctt ttatcgtttg atacatgagg tcgtggaatt
      841 cggtccgacc ccgtttatac gacgtcgtat tcatattcgc taaatcgttg gagatctgat
      901 ctaatttagc ttcttgcgcc tgcatacctg ttgcagctgt tgataaagat cgaatcataa
      961 tttacctacc tcattcgctt cttttactgc catttcgtta tatgccttga tggctttcat
     1021 attggattcg aacaaccgat tcgcttttat gacattcata atttcggata ccgcgtttac
     1081 gttgctaccc tcgataaaac cttggtgaat tcgcgaacct cgagcaacac tcgctacatt
     1141 tgtggggcta ggattggtaa aaatcgcagc tccttttttt aatagttggc taggatttga
     1201 aaattcggcc atcgcgatct gcgcaatttc ctcattgcct acaaaaattt ttccgtcgtc
     1261 ggtgatgcga agtttggatc cggaactcat cgcttcagct aagttaatgg gctgcagccc
     1321 gcctggttgt gcttggtcct ggggcacagc gcctggggcg ttctgctctc cgccacccca
     1381 aggcggaagc gcgccactaa acggacttgc agcggctgga gctctaccta cttctgcctg
     1441 agcatccgct gtttcaccgc cagcttcggc ggcggcgcca gctagtgaga gcacgcggta
     1501 accgtcgttg gtcattaact gaccgttacc ggcaatttta aagtcaccgg ctcgggtaaa
     1561 ggcgagaccg tttggcgttt ggactgcgaa ataccccggg ccttctatag ccaagtctaa
     1621 ggggccgttt gtttgtcgga aagttccctg cgagtggtcc gtaaccactg cgtcaacatt
     1681 gaccatcgca tgctcgcggc cgtcatgatg gtaaaagtca gagtctctaa agatcgttcg
     1741 gggaatatcg acatcgggcg acggcggcct ttcgtgaatt gtcaggtact ctttgaacgt
     1801 aggttcatct tttttaaagc ccgcagtatt tgcgttagct aagttatttg cagcgatatc
     1861 gatggcctgg ctttgcgcca tgctacccga aaccgaagtc cataatccag taggtgaacc
     1921 catgtggtcg ctttccccca atctctaaga aagcaaactg t
//
LOCUS       DAOMXO010000472         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_34241_length_1959_cov_3.432773, whole genome shotgun sequence.
ACCESSION   DAOMXO010000472 DAOMXO010000000
VERSION     DAOMXO010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34241_length_1959_cov_3.432773"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLH57_09270"
     CDS             <1..406
                     /locus_tag="PLH57_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRK02843.1"
                     /translation="QETLKMIKDEGLENVFKRHETLARATRAGIKALGLEMLAKDSPS
                     IAMTAVLVPSEIKDGKGIPKTMRDKYGVTIAGGQDELEGKIFRLTHFGYVDKFDILIG
                     IGALELTLKDLGYTKFEFGAGTGAVLKALASS"
     gene            complement(403..1131)
                     /locus_tag="PLH57_09275"
     CDS             complement(403..1131)
                     /locus_tag="PLH57_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRK02844.1"
                     /translation="MNIVRLFGGAGFLINLLVNTGWADQRGTLATVVKRRWGDAVVRI
                     VVKSKSKKELAYGSGFFISNNGVLVTNFHVIEPFVKVKTSRLEFELFDGRIVRDFEVG
                     NCEKRVLTDLCILRLAVEPEKHFTLDSREPIKGESVFNIGNPGKSKFRVGSGKYLGLY
                     KVRNGGIGYISFTNPIRPGDSGGPIFTIEGQLVGVATLRISFGNNKTTKHFVGVGAHE
                     VARLRKTFSRFEPWQKHFARLGLR"
     gene            1290..>1959
                     /locus_tag="PLH57_09280"
     CDS             1290..>1959
                     /locus_tag="PLH57_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02845.1"
                     /translation="MFLLFLIAIWAPATAFSGDFQENVLFCGTAAGSYTIGDERLPLF
                     KATQDQNGDQYVKPDERSSSKERTYRIESFSYLEDGVVRIHRKNKSDLERSASAGLMA
                     EFDLRSVMNQKIRGTEICIFGDLISNAYTNQYYIRPRRFVVYTNALSFLVPSDLDGVY
                     FGKAKHKSDKIELETRVSSLDFQSITESLISTSGAPCAFPSADENDSVTVSRISAKEL
                     SVKIL"
BASE COUNT          540 a          489 c          435 g          495 t
ORIGIN      
        1 gcaagaaact ttgaaaatga tcaaggacga gggtttagaa aacgtcttta aacggcacga
       61 aacactcgct cgcgccaccc gtgcgggaat taaggctctg ggtcttgaaa tgctcgctaa
      121 agatagcccg tcgattgcca tgaccgcggt gcttgtgccc tcggaaatca aagatggcaa
      181 gggcattcca aagaccatgc gtgacaagta tggcgtcaca atcgccggcg gccaagacga
      241 actcgaagga aaaatctttc gactcaccca ctttggttat gtcgacaaat tcgacattct
      301 cataggtatt ggagctctcg agctcacgct caaagacctg ggctacacta agtttgaatt
      361 tggcgcaggt accggtgcgg tgctcaaagc actagcttcg agctagcgaa ggcctagcct
      421 agcaaagtgt ttttgccagg gttcgaaacg cgaaaatgtt ttacgtaagc gagctacttc
      481 gtgggcgccg acgccgacaa agtgtttggt agttttgtta ttaccaaaac ttattcttag
      541 cgtagccaca ccgacgagtt gaccttcgat tgtaaaaatc ggtccaccgc tgtcgcctgg
      601 cctgatagga ttggtgaaag agatataccc gattccaccg ttacgaacct tgtagagtcc
      661 aaggtatttg cccgaaccaa cccgaaactt ggatttacca ggatttccga tgttaaagac
      721 ggactctcct ttgatcggtt cacgcgagtc cagcgtaaag tgcttttcag gttcgaccgc
      781 cagtcgcaaa atacaaagat ctgtaagaac ccttttttca caattaccaa cctcaaagtc
      841 acgaacgatt cgaccatcga atagttcaaa ttcgagtcga ctagttttca cttttacaaa
      901 cggttcgatg acatgaaaat tcgtaaccag aacgccgtta tttgaaatga aaaaacccga
      961 accgtaggcg agctcttttt tagactttga ttttacaact atacgaacga ctgcatcgcc
     1021 ccaccttcgt tttacaactg tggcaagcgt ccctctctgg tcggcccatc ccgtgtttac
     1081 gagcaagttt attagaaaac cagccccccc gaatagacgc acaatattca taagttctag
     1141 tttaaggtct taaaagcgct aataaaaccg gataaacttg tccgcattaa agtgcacact
     1201 tagcccttat ttcaatacta ttttgatcaa caattaaact gactatttta cttagaaaac
     1261 aagagcaaat cgtgttatag ctcgcccgaa tgtttttgct gtttttaatc gctatttggg
     1321 cccctgcgac cgccttttcc ggcgatttcc aagagaacgt gctgttctgt gggaccgcgg
     1381 cgggctcgta caccataggc gacgaacggc taccactctt caaagctacc caggatcaaa
     1441 acggtgacca gtacgtaaag cccgacgaac gttcgtcgag caaggaacgg acctatcgaa
     1501 tcgagagttt tagttatctc gaagacgggg tggtacgcat ccaccggaaa aacaaatccg
     1561 acctggaacg ctctgcatcg gctggtctca tggccgagtt cgatcttcgt tcggtgatga
     1621 atcaaaaaat cagaggcact gagatttgca tttttggtga tctcatttcg aatgcctaca
     1681 ccaatcaata ttacattcgc cccagacgct tcgtggtcta caccaatgca ctgagctttc
     1741 tagtaccttc ggatctcgac ggagtctatt tcggaaaagc taaacacaag agcgacaaaa
     1801 tagaactcga gaccagggtt tcatctcttg attttcaatc gatcactgag tctttgattt
     1861 caacttccgg cgccccttgc gcgttcccga gcgcagacga gaacgattcg gtgaccgtgt
     1921 ctcgcataag cgcaaaggaa ctatcggtaa aaatcttac
//
LOCUS       DAOMXO010000473         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_34294_length_1957_cov_3.364353, whole genome shotgun sequence.
ACCESSION   DAOMXO010000473 DAOMXO010000000
VERSION     DAOMXO010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34294_length_1957_cov_3.364353"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PLH57_09285"
     CDS             <1..770
                     /locus_tag="PLH57_09285"
                     /inference="COORDINATES: protein motif:HMM:NF017697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HRK02846.1"
                     /translation="SVLMSVNITLRKIYTFAATFLVAGGAAYANNTHGNHIDRFDSFF
                     ELRKEFTEDRDFRVAAILRRSPILVLAIHGGNIEPGTSELADLIAGGEHSLYKFESLL
                     PEEPAWDHHVPSTLFDEPRALEMLERASTSVSVHGFYWFGNPIENERATVILGGQNSL
                     LRDKIAARLEDFAERTMPSLHILPWEEARKFAGMHPRNIVNRTAQQGVQIELSRSLRK
                     IFMDHPELAKSFARSVRLAIDAHRKSGFCKELFNETF"
     gene            complement(754..>1957)
                     /locus_tag="PLH57_09290"
     CDS             complement(754..>1957)
                     /locus_tag="PLH57_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02847.1"
                     /translation="LALSASPLMCRDLEEFNELAGISAQVDDFQDRIVEEAAVLEVVY
                     GMCKGLKTASSKWKRSRGKKGGLDLERKLLASLRAHSDLPKFALYEKWPGCPCDKDKS
                     SRKIKGVCFKGLTPYDPIDERVPQLLERYAEKLQAWAVANGMTLSSVQMGGCSFARLA
                     RAGGHEDGTFDHSDISSHAESSACDLYSATFEETVKSGKDRVKTKTKTVKFFDLRSRK
                     RKKIQSHPFADAFQKYLNWMDKEKPLIERMAFKRHDVELKREIVKRVNSSKDRTKGKH
                     HDIEEKKSKSRIPEAIYDYRKLADELEKNGCPLKDENSCDQLRLGFLLINAGRDVGFL
                     TIAPGVGSHDGHVHLGASPDEQDSLRLTSGLMPTECHDKLIDYYVIRAAGLELPYPQC
                     AERFKMSR"
BASE COUNT          485 a          503 c          441 g          528 t
ORIGIN      
        1 gatcggtctt aatgtcggta aatattactc ttcgaaaaat ttacacattt gctgctacct
       61 ttttggtggc cggcggagcc gcctatgcga acaatactca cggaaaccac atcgatcgat
      121 tcgatagttt ttttgaacta cgaaaagaat tcaccgaaga ccgcgacttt cgagttgcgg
      181 ctattttgcg acggtcgcca attttagtgc tcgcaattca cggcggtaat atcgaaccag
      241 gcacgtcgga actcgccgac ttgatagccg gcggcgaaca ctcgctgtat aagtttgaat
      301 cccttcttcc cgaagaaccg gcctgggatc accatgttcc cagcactttg ttcgacgaac
      361 cccgagccct tgaaatgctc gaacgagcct ccactagcgt gagcgtgcac gggttttact
      421 ggttcggaaa cccaatagaa aatgaacggg ccactgtgat cttgggcggt caaaacagtt
      481 tattgcgaga taaaatcgcg gctcgtctag aggacttcgc agaacgaacc atgccaagcc
      541 ttcacatttt accgtgggaa gaagctagaa aatttgcggg aatgcacccg cgaaatattg
      601 tcaatcgaac cgcgcaacag ggcgttcaga tcgaattatc cagaagtctg agaaaaattt
      661 ttatggacca ccctgaatta gcaaaatcat tcgcgcgaag cgttcgcctg gcgatcgacg
      721 cccatcgaaa atctggtttc tgcaaagagt tgttcaacga gacattttaa agcgctcggc
      781 gcactgcggg tacggcagtt caaggccggc ggctcgaatc acgtagtagt cgataagttt
      841 atcgtgacac tcggtgggca tcaacccgct ggtcaaacga agagagtctt gttcgtcggg
      901 cgaagcacct aagtgaacgt ggccatcgtg actgccgaca ccgggggcaa tcgtgagaaa
      961 ccctacatcg cgtcctgcat ttattaaaag aaatcccaag cgcagttgat cgcaggaatt
     1021 ttcatctttt agcggacaac catttttttc cagctcatcg gccaattttc ggtagtcata
     1081 aatcgcctcg ggaattcggc tcttcgattt tttttcttca atatcgtggt gtttgccttt
     1141 agtgcgatct ttcgaactat ttacccgttt cactatttcg cgttttaatt ccacatcatg
     1201 acgcttaaat gccattcgct cgattaatgg tttttcttta tccatccaat ttaaatattt
     1261 ttgaaacgcg tctgcaaaag ggtgtgactg aatttttttt cttttcctac ttcgtaggtc
     1321 aaaaaattta accgttttgg ttttagtttt aactcgatct ttacccgact ttaccgtctc
     1381 ttcgaaagtg gcactgtaca gatcgcacgc actcgactcg gcgtgagaac tgatatcact
     1441 atgatcgaat gttccgtctt catgaccgcc ggcacgcgcc agacgagcaa aggagcaacc
     1501 acccatctga accgaactga gcgtcatacc attggccact gcccaggcct gaagtttttc
     1561 tgcatatcgc tcgagcaact gcggtactcg ttcgtcgatc ggatcatagg gggtcagccc
     1621 cttgaaacaa acccctttga tcttacggct cgacttgtct ttgtcacacg gacaaccggg
     1681 ccacttttcg taaagggcga acttaggtaa atctgaatgc gctcgcaaag aagcgagaag
     1741 ttttcgttca agatctaatc cgcccttttt accccgcgat cttttccatt tggaagatgc
     1801 ggtttttagc cctttgcaca taccgtaaac cacttcaagc accgcggctt cttctactat
     1861 tcgatcctga aagtcatcga cctgcgcgga gatccccgct agttcattaa actcttcgag
     1921 atctcggcac atcagtggtg acgcgcttag agccagc
//
LOCUS       DAOMXO010000474         1951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_34427_length_1951_cov_5.396097, whole genome shotgun sequence.
ACCESSION   DAOMXO010000474 DAOMXO010000000
VERSION     DAOMXO010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34427_length_1951_cov_5.396097"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PLH57_09295"
     CDS             complement(<1..1350)
                     /locus_tag="PLH57_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRK02848.1"
                     /translation="MPCIASQVVNKLERFGLSVVTVLISVVCSLVFVSRGMGAEELLP
                     PPSLVEPKTCTAASFCKNNKRQFDWVSRAVGADLVVRELVRIGVLPGHTTVAVVDSGF
                     DFANRSQSIVSPEVKLMCSGSECIRTDLNGHGTSVAGMISGKDFGVAPDVSLLVVPAG
                     SGDTTNWTILVKDEELAILRACEAGAEVINVSYSSLEDEVGLRTEWLMPETIDELRKK
                     GCLLVRAAGNSSLQRDVGRKYFDSDDALLRVEATNWAGSRSSISSAGEILAPGEDVFT
                     LATQKHSEELISDKYFFDIMGCESQPHGYFTSGTSVAAPVVAAIAAQVMGVAKVHGLW
                     SILDASERVKWTNRVVLASRLNNQINALRAVTLADAVAKDWVKKKNTTKRKSEFESYV
                     SRAFAEKNLMAAYKVAVKPFCTSALKNLVTDCSATNCEEFTSCLNQLRKVAQLCDGAS
                     "
     gene            1551..>1951
                     /locus_tag="PLH57_09300"
     CDS             1551..>1951
                     /locus_tag="PLH57_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02849.1"
                     /translation="MRTTYAALTVLTLVGLSGCSPKASEGIPSNTKAQISSGSIVAGP
                     TPSPVPPITPPPFPPPTPEPTPVPAGNPPLSFTVTGKGNHPKHVCIAADETLKIRIKP
                     PAAVSNDPIISRWPAPTRTYIALRMKVAIQGA"
BASE COUNT          477 a          551 c          422 g          501 t
ORIGIN      
        1 ggatgcgcca tcgcaaagct gtgctacttt tcgcagttga tttaaacacg aggtaaattc
       61 ctcacaattt gtagcactgc agtcagtaac taagttttta agcgctgaag tgcaaaaggg
      121 tttcaccgct actttgtagg cagccattag atttttttcg gcgaaagccc gacttacata
      181 gctttcaaat tctgatttgc gtttcgtcgt atttttcttt ttaacccaat cttttgctac
      241 tgcatcggcc agagtgactg ctctgagagc gtttatctga ttattcaatc gtgatgcgag
      301 caccacgcga ttggtccatt ttactctttc cgacgcgtct agaatgctcc atagtccgtg
      361 aactttggct acacccatta cttgagccgc gatggccgcc acaactggtg ctgccacact
      421 ggttcccgaa gtgaaatacc cgtgcggctg cgactcgcaa cccataatat cgaaaaaata
      481 cttgtcgctg attagttcct cagaatgttt ctgggtggcc aaggtaaaca catcttcacc
      541 gggagcaaga atttcaccgg cggaagaaat actagatcga ctacctgccc agttggtagc
      601 ctctactctc aacagtgcat catccgaatc gaaatacttt ctaccgacgt ctcgctgcag
      661 ggaactattt ccagcggctc tcacaagtag acaccctttt ttgcgtaact catcgattgt
      721 ttcgggcatg agccactcgg ttctcaaacc aacctcatct tccaaagaag agtaacttac
      781 gttaatcacc tcggctccgg cctcacaggc tctaaggatt gccaattctt cgtctttcac
      841 taaaatagtc caattagtgg tgtcgcccga gcccgcgggt actaccaaaa gtgaaacatc
      901 cggagcgacc ccgaaatctt tgccgctgat catgcctgcc acgctcgttc cgtggccatt
      961 taaatcggtc cgtatgcatt cgctgccaga acacatcagc tttacttcgg gtgacactat
     1021 ggattgggat cggttggcga aatcgaagcc ggaatctacg accgcaacag tggtgtggcc
     1081 ggggagcacc ccaattcgaa ctagctccct aacaaccaaa tctgcaccga ctgcgcggga
     1141 aacccagtcg aattgtcttt tattgttttt acaaaagctc gctgcagtgc aggtttttgg
     1201 ttcaaccaaa cttggtgggg gcaaaagttc ttcggcgccc atacctcgcg aaacgaacac
     1261 gaggctgcac accaccgaaa tgagtaccgt aactactgat aatccgaatc gctccaattt
     1321 attcaccacc tgactagcaa tacaaggcac gtaccacaat tgaaccctcg attggcagtc
     1381 atcaatatac cgcccacccc tcacagtcgc tgcaataacc agacgggtgt ctaaagttta
     1441 gacactgttt ttaccgggtt tttaggggtt ttttccgatt ttgccctggt ctcgcttgct
     1501 cgaccaatgg cacccccatt gcaaactata gagcttagag agagggattc atgcgtacta
     1561 cttatgcggc tctgaccgta cttacactcg taggcttaag cggctgctcg cctaaggctt
     1621 ccgagggaat tccgtcaaat accaaggccc aaatcagctc cggaagcatc gtagcaggtc
     1681 ccactccgag tccggtacct ccgattacgc ctccaccatt tccgccgcca accccagaac
     1741 cgaccccggt tccggctgga aatcccccgt tgagttttac tgttaccggc aagggcaacc
     1801 accccaaaca tgtttgtatt gcagccgacg aaactctaaa aattcgaatt aaaccgccgg
     1861 ccgctgtttc gaatgatccg atcatctcac gttggcctgc ccctactcgt acctatattg
     1921 cgcttcgcat gaaggtagcc attcagggtg c
//
LOCUS       DAOMXO010000475         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_34480_length_1949_cov_5.002640, whole genome shotgun sequence.
ACCESSION   DAOMXO010000475 DAOMXO010000000
VERSION     DAOMXO010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34480_length_1949_cov_5.002640"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLH57_09305"
     CDS             complement(<1..485)
                     /locus_tag="PLH57_09305"
                     /inference="COORDINATES: protein motif:HMM:NF019603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02850.1"
                     /translation="MNSVIHLVGRGSQGRAWDECLRRTGRQVLVYHRGNSASLPSGLS
                     SRSRDQVQIVVLACADDAIASVYNEHLRSVDAPLLCVLLHGFAVYSGSLPKTALASNH
                     ALALLAPKAIGPELRTEFFEHFPHPHDLKAALHLEANSELLRETFREALSTLARDLGF
                     AT"
     gene            520..876
                     /locus_tag="PLH57_09310"
     CDS             520..876
                     /locus_tag="PLH57_09310"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRK02851.1"
                     /translation="MQRTMLKSKIHRATVTDANLEYEGSITIDKDLLKQADIWVFERV
                     EVYNVDNGARFATYAIEGDAGSGTICVNGAAAHLVKPGHKVIIASYVNMEESDIPKYQ
                     QRIVLVDSKNKAKSSL"
     gene            complement(1063..>1949)
                     /locus_tag="PLH57_09315"
     CDS             complement(1063..>1949)
                     /locus_tag="PLH57_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02852.1"
                     /translation="SDSDENTPEEKKEDGVVSKGMKNQSLGVIYMGVGSSNPVVFENG
                     MESSGNVGLGIFRHGGYLGAGLAVNPSSQERIGRRETDDALVTTDGELVEIEGDRCTV
                     VDADGRTVRECSEKAKANLNVQAVALPVWRSDKCEFDGQNSCWALTLSGWAETGFSDD
                     TYGVGSVQLRKMKMLGEKLMVHIGAGGTIARGGKTDLTGLGFGGGFAVQSGKATVVTD
                     VTYGIDAFGGYKKNVITGSVAGHYDTKHGDIFVNLSGEDYEAYAPKDPERKGVRGIIG
                     IAGSHDYASGDSGAKKAD"
BASE COUNT          452 a          523 c          511 g          463 t
ORIGIN      
        1 gtcgcgaatc caagatctcg agcaagtgtg ctcagcgctt cgcggaatgt ttcgcggaga
       61 agctctgagt tcgcctcgag gtgtagcgca gctttcagat cgtgggggtg tgggaagtgc
      121 tcgaaaaact cagtacgtag ttcgggcccg attgccttgg gcgcaagtaa tgccaacgcg
      181 tgattagagg cgagggctgt tttgggtagg gaaccagagt acaccgcaaa accgtggaga
      241 agcacacaca ggagcggagc gtcgaccgag cggagatgtt cattgtagac cgaagcaatg
      301 gcatcatcgg cacaggccaa gaccactatt tggacctggt ctcgtgatcg agagcttagc
      361 ccgctcggca gggatgcgga atttcctcgg tgatagacca gcacttggcg gcccgttctc
      421 cgtaaacact cgtcccaggc ccttccttga ctaccgcgcc ctactaaatg tatgactgaa
      481 ttcattttag tctccagccc cgctttttaa gggagtttta tgcagagaac aatgctcaaa
      541 tcaaaaatcc accgcgccac cgtaaccgac gcaaatttgg agtatgaagg gtctattacc
      601 atagataaag accttctaaa acaggcggat atttgggttt tcgaacgcgt tgaggtctat
      661 aacgtagaca atggagcgcg gtttgccacc tatgcgatcg aaggggacgc tggttcaggc
      721 acaatttgtg tgaacggcgc ggccgctcac ttggtaaaac ctggccacaa agtgatcatc
      781 gcatcttacg tgaacatgga agagtctgac attccaaagt accagcagcg catcgtgttg
      841 gtggattcca agaacaaagc aaaaagcagt ctgtgaccag ctcgcttgag cagacgttgt
      901 ccgccgcgca aagtccgccg cgcaaagtcc gccgcgcaaa gtccgccgcg cgaagtccgc
      961 cgcgcgaaga tcgccgcgcg aagatcgccg cgataagcgc aatgcaaaaa cccctcgcat
     1021 gccgaatgcg aagggttttt atattagaac caagtaggtg atttagtcgg ccttttttgc
     1081 gccgctgtca ccgcttgcgt aatcgtgcga accagcgatc ccgattattc cacgaacgcc
     1141 cttacgttca gggtctttcg gggcgtaagc ttcgtagtct tcgccgctta agttcacgaa
     1201 tatatcgccg tgtttcgtgt cgtagtggcc tgctacgctg ccagtgatta cgtttttctt
     1261 gtaacctccg aaagcgtcga tgccgtaggt aacgtctgtt acaacggtcg ccttgccgga
     1321 ttgtactgca aaaccgccac caaagccgag accagtgaga tcagtttttc cgccgcgggc
     1381 gatagttccg ccagcgccga tgtgaaccat taatttttcg cccagcattt tcatttttcg
     1441 aagttgtacg ctgccaactc cataggtgtc atctgaaaat ccggtttcag cccagccgct
     1501 gagagttagc gcccagcaac tattttggcc gtcgaactca cacttgtcgg agcgccatac
     1561 tggaagtgca actgcttgaa cgttaagatt cgccttagct ttttcggagc attcgcgaac
     1621 ggtgcggccg tcagcatcaa ctacggtgca gcggtcgcct tcaatttcta cgagctcccc
     1681 gtcggttgta acgagcgcgt cgtcggtttc gcgacgacca attcgctctt gagacgaagg
     1741 attcaccgcg agtcccgcgc ctaaataccc tccgtgtcta aaaattccga gtccgacgtt
     1801 gccggacgat tccatgccgt tttcgaaaac cacgggatta gaagaaccaa cgcccatata
     1861 gattacgccg aggctttggt ttttcatgcc tttagaaaca acaccgtctt cctttttttc
     1921 ttcaggggtg ttttcatcgc tatctgaag
//
LOCUS       DAOMXO010000476         1944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_34619_length_1944_cov_3.823187, whole genome shotgun sequence.
ACCESSION   DAOMXO010000476 DAOMXO010000000
VERSION     DAOMXO010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34619_length_1944_cov_3.823187"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLH57_09320"
     CDS             complement(<1..263)
                     /locus_tag="PLH57_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02853.1"
                     /translation="MIDETEPTIENAVTEESAEEATTSPAVSEEQLLELAQKEGLADG
                     LESGSDETPEALEASAKAVAELTDEQRAQTKAAIETILFMSDRP"
     gene            complement(260..1051)
                     /locus_tag="PLH57_09325"
     CDS             complement(260..1051)
                     /locus_tag="PLH57_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRK02854.1"
                     /translation="MELLKVKTDQFEGPMDLLLFLIQKHELDVSAISIHLITDQYVQY
                     VEMMKDLNFDSASEFLLMAATLIHLKSKRLLPEDESLATGSSDDGLPRSEAELVRRLL
                     EYKKFQEAGRRLYELPRLNESVFSRPGVQPPEKQTVWREMDMTGLTLAFQDVLKRSRK
                     RTRIIIREPTSIPERIAELGKIMKVDEMTEFASLLSSEADRAEITVTFIALLELARLK
                     KIKVYQNETFGSIYIKLLEPLAEFDPTLATGFQYNVRQIEELAKA"
     gene            complement(1134..1823)
                     /locus_tag="PLH57_09330"
     CDS             complement(1134..1823)
                     /locus_tag="PLH57_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRK02855.1"
                     /translation="MFENFGERVQMLFLQMVPFLMAIVIHEVAHAFVARRHGDHTAEK
                     LGRLTLNPVPHIDPIGTLLFPAVNILTGIPILFGWAKPVPIDYNRLKPYRRGLFLVAL
                     AGPASNFLLGFLAALFLVAFSSLVPSSFYLYEPLRGMAFVAISINYALGLFNLVPLPP
                     LDGSKMVESFLSYEATRQYEKLAAYSFFILLALLWSGALSVLAIPINFLSNLSIGIWA
                     AIFGQLGLISM"
BASE COUNT          490 a          440 c          536 g          478 t
ORIGIN      
        1 gggcgatctg acatgaacaa tatagtttcg atagcggcct tggtttgagc gcgctgttca
       61 tcggttaatt cggccaccgc tttggcgcta gcttcaagag cttctggagt ttcatcggaa
      121 cccgattcaa gtccatcggc caggccttct ttttgggcaa gctcaagaag ctgttcctca
      181 gaaacagccg gagacgtagt cgcttcttcg gcagattctt cggtaactgc gttttctatg
      241 gtgggttctg tttcatcgat catgcttttg ccagctcctc gatttggcga acgttatatt
      301 gaaatcccgt agcgagcgtg gggtcgaact cagcgagcgg ttccagaagt ttgatgtaaa
      361 tggagccaaa cgtttcgttc tgatagacct tgatcttttt caggcgcgca agctccaaga
      421 gcgcaatgaa agttactgtg atttcggcac ggtcagcttc gctcgataaa agcgacgcga
      481 attccgtcat ttcatcgacc ttcatgattt tccccagctc cgcgatacgc tcgggaatag
      541 aggtcggctc acgaatgatg atgcgagtgc gtttgcgcga acgcttgagt acgtcttgaa
      601 atgcaagcgt aaggcccgtc atgtccattt cgcgccacac ggtttgtttt tcgggtggtt
      661 gtacgccagg gcggctgaaa accgattcgt ttagccgcgg gagttcgtag aggcgtcggc
      721 cggcttcttg aaattttttg tattcgagta atcgacgaac gagttcggct tcggaacgag
      781 gaagtccgtc atctgaggaa ccagtcgcca gcgactcatc ttccggaagt agacgtttgg
      841 attttaggtg tattagcgtt gccgccatca gcaaaaactc gctggcggaa tcaaagttga
      901 ggtctttcat catttcgacg tattgcacgt actgatcggt gatcaggtga atgctaatag
      961 ccgacacgtc tagctcgtgt ttttgaatca aaaacaacag caagtccatg gggccttcga
     1021 attgatcggt tttgactttt aagagttcca agcacgacct cgtgtgtaga ctatcgaacg
     1081 atccctatgc ttgacaagca gtattgcgaa agaattggtc ggattaaagg gacctacatg
     1141 gagatcaggc ccagttgtcc gaaaatagcg gcccatattc cgattgagag gttgctaaga
     1201 aagtttatgg gtatcgccaa aacgctcagg gccccactcc aaagcagagc taacaaaata
     1261 aaaaagctat aagccgcaag cttttcgtat tggcgggtgg cttcgtaaga taaaaaggac
     1321 tccaccattt tggacccgtc gagcgggggc agcggaacta gattaaaaag tcccagcgcg
     1381 tagttgatcg aaatagcaac aaaagccatg ccgcgaagag gttcataaag gtaaaaactc
     1441 gaaggcacga gcgagctgaa agcgaccaaa aacagagcgg ccaggaatcc aagcagaaag
     1501 ttagaggccg ggccggctag cgcgaccaga aataggccac ggcgataggg tttcagccga
     1561 ttgtagtcga tcggaactgg ttttgcccaa ccgaaaagaa tgggaattcc ggttagaatg
     1621 ttaacggccg gaaagagcag cgtgccgatt gggtcaatgt ggggcactgg gttgagcgtg
     1681 aggcgtccga gtttttctgc agtatgatcg ccatggcggc gcgctacgaa ggcgtgggca
     1741 acctcgtgta tcacgatggc catcaagaat ggcaccattt gcaaaaagag catctgaacg
     1801 cgttcgccga aattttcaaa cattattact ccagtgtcgg ttcaaaatat tgaatgtggt
     1861 gccgagagta acactcaagg ctcggtttat cgagtgccaa atcgtttgct cttggagcgc
     1921 gggtgaaagg tttcgagagt ttcg
//
LOCUS       DAOMXO010000477         1943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_34644_length_1943_cov_4.075212, whole genome shotgun sequence.
ACCESSION   DAOMXO010000477 DAOMXO010000000
VERSION     DAOMXO010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34644_length_1943_cov_4.075212"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PLH57_09335"
     CDS             <1..1142
                     /locus_tag="PLH57_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRK02856.1"
                     /translation="LLEDAALLCGARPWNAFFKITLPIARSQLLSCLLLTMASSLAAF
                     GVPAMLGIPSRTYFVTTKIYQGVKSGSDFSQVSFLAATLMVLTAILVFAHDRAAKRTP
                     HSTIVGGKASQRNLLRPTTGSRIFGVGVFFFCFFGTLAPLVAVFLQSLLVDRGSFSNW
                     SLAKYQYVFGSVPDGVRAAQNSLWIAFFTATILTGLGLVAAYSKTKTTGFSQKIAGHV
                     ANVWSFAYATPGTVLALPILVFFQPSLGNTLWIIGLAFGVKYAAFAIRTLEPALTQIS
                     RDLEEAAWISGATPARTFVRIVIPLCIPALSAAFALCFIPMLSELTMSVFLVGAGTET
                     LGTLIYRFQEYADPGAASVLAVSVAGFTLLLNGALKKFSRGGFGI"
     gene            1139..1906
                     /locus_tag="PLH57_09340"
     CDS             1139..1906
                     /locus_tag="PLH57_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK02857.1"
                     /translation="MKWGLEIAGISKKYQSSQNAAPSQGFALDQVHFSVNPGELVTLL
                     GPSGSGKTTLLRIIAGLEIPTTGRVTLSHDANLVTFVDVEKDVYLEPFQRNVGMVFQS
                     YALWPHRTVAENVAFPLEIQKIGRDERDKLIHEVLGRVELLGFEHRFPHELSGGQQQR
                     VALARALVQRPRLLLLDEPLSNLDVALRASVRKEIKELNRKLGLTMIYVTHDRTEALE
                     LSDRMCIFDRGRLIQIGTPEEITAHPATDFVRQLVTS"
BASE COUNT          440 a          495 c          464 g          544 t
ORIGIN      
        1 ctttactgga agacgctgca ctactttgtg gcgctcgtcc ctggaatgct ttttttaaaa
       61 tcaccctccc aattgcgcga tcacaacttt tatcgtgcct gttattaacc atggcgtcga
      121 gcctcgccgc ttttggcgtg cccgcgatgc taggtattcc gtctcgaact tactttgtta
      181 ctacaaaaat ttatcaggga gtaaaaagcg gatcagattt ttcgcaagtt tcatttcttg
      241 cagcgaccct aatggtgctc accgccattc ttgttttcgc ccacgatcgc gctgccaaac
      301 gaacgcctca ttcgacgatt gttggtggca aagcttcaca acgaaaccta cttcggccga
      361 cgaccggcag tcgcatcttt ggggtcggcg ttttcttttt ttgttttttc ggaacgctcg
      421 ctcctctggt tgccgtgttc ttgcaatcgc tcctggtaga tcgcggaagt ttttcgaatt
      481 ggtcgctcgc gaaataccaa tatgtttttg ggagcgtgcc cgacggcgtg cgtgctgctc
      541 aaaacagtct ttggattgca ttttttacgg ccactatatt gactggatta gggttggtgg
      601 ccgcttattc taaaacaaaa acaacgggtt tctctcaaaa gattgccggc cacgtagcca
      661 acgtatggag tttcgcctac gccactccag gcactgttct cgcacttccc atattggttt
      721 tcttccaacc gtcgttgggc aatacacttt ggattatcgg cctggctttt ggggtaaaat
      781 acgcagcttt cgccattcgt actctcgaac cagccctcac tcagataagc cgagaccttg
      841 aagaagccgc ttggatatca ggcgcaacgc cggctcgcac ctttgtccgt atcgtgatac
      901 ccctttgtat accggcgtta tcggcggcgt ttgccttgtg ttttattccg atgctatcgg
      961 aactaaccat gtcggtattt ttggttggcg caggaaccga gacactcgga actctgatct
     1021 atcgttttca ggagtacgcc gatccgggtg ccgcttcggt gctcgcagtt agtgtcgcag
     1081 gttttacgct cttacttaat ggtgcactca aaaagtttag tcgggggggg tttggcatat
     1141 gaagtggggt ctagaaatag cgggaatctc taaaaaatat caaagctccc aaaacgctgc
     1201 tccgtcgcag ggttttgcct tagatcaagt tcatttttcg gtaaacccag gagaactcgt
     1261 aactttgctc gggccgagtg gaagtggaaa aacgactttg cttcgaatca ttgcgggctt
     1321 agaaattcca accaccggcc gagtcaccct aagtcacgac gccaacctgg tcacctttgt
     1381 cgatgttgaa aaagatgtct atcttgaacc gtttcagcgc aatgtaggta tggtttttca
     1441 aagttacgct ctatggcccc atcgaaccgt cgcagaaaat gtggcattcc cattagaaat
     1501 acagaaaatt gggcgagacg aacgggataa attgatccac gaggtacttg gacgagtgga
     1561 actactaggg ttcgagcacc gatttcccca cgaactctct ggtggccaac agcaacgcgt
     1621 cgcgttagct cgagccttgg ttcagcgccc gcgcctgctc ttgttggacg aaccattgtc
     1681 gaatctcgac gttgctttac gtgcttccgt gagaaaggaa atcaaggagc tcaaccgtaa
     1741 acttggcctt actatgattt acgtcaccca tgatcgaacc gaagcgttgg aactttcaga
     1801 tcgcatgtgt atttttgatc gcggtcgact gattcaaata ggcacccctg aagaaattac
     1861 cgcccacccc gcaacagatt ttgtgcgaca gcttgtcacg agttaactcg taaaaatttc
     1921 gatgctacaa tttaccccat tgg
//
LOCUS       DAOMXO010000478         1942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_34668_length_1942_cov_4.632220, whole genome shotgun sequence.
ACCESSION   DAOMXO010000478 DAOMXO010000000
VERSION     DAOMXO010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34668_length_1942_cov_4.632220"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLH57_09345"
     CDS             <1..411
                     /locus_tag="PLH57_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02858.1"
                     /translation="VSYDRLKSDGALSVVGRTDGMIELRDGFNHELVEVNQVFPEGRY
                     VTTVKLSKSGDIVFAGDSSGYVFILETLSTPALATLFEIPVPKVVAAEGMAGREVVAI
                     ELDPESEPKWMRVTYRSGHVHLLELLGESNADLN"
     gene            441..1178
                     /gene="kdsB"
                     /locus_tag="PLH57_09350"
     CDS             441..1178
                     /gene="kdsB"
                     /locus_tag="PLH57_09350"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRK02859.1"
                     /translation="MQKKICIVIPARLGSTRLPEKALADIHGKPMIVRVLERARQING
                     VHEIIVATDHERIVAAVEAAGGTAVMTPDSLQSGTDRVAFVANERTADIFVNVQGDEP
                     LLDPKAVERTLALIEGGRFTMATPAAPIRHRDDLKNSNIVKVLVASDGRAAYFSRFPI
                     PYSRGDIPADLGALAPLQHIGVYAYTRETLLKFWKLERTTWEVAESLEQLRALYHGIP
                     IGVALTEKASTGVDTIEDLERVRSLMV"
     gene            1186..>1942
                     /locus_tag="PLH57_09355"
     CDS             1186..>1942
                     /locus_tag="PLH57_09355"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRK02860.1"
                     /translation="MAAKPKQKKYVFVTGGVLSSIGKGIAAASIGYLLENRGVKVTMI
                     KMDPYINVDPGTMSPFQHGEVFVLEDGAECDLDLGHYERFVNCTLTRENNFTTGKVYD
                     TVIQKERRGEYLGKTVQVIPHITDQIKENVRKAAADADLAIVEIGGTVGDIESLPFLE
                     AIRQFKAEVGAENVLYVHVTLVPHIAAASELKTKPTQHSVKELREIGIQPDILLCRSD
                     RALPKDVKDKIALFCNVTPDCVFSAMDVQTIYEV"
BASE COUNT          506 a          437 c          521 g          478 t
ORIGIN      
        1 gtcagttacg atcgattgaa gtccgacggc gctctttcgg tggtcggccg aaccgacgga
       61 atgatcgaac tgcgcgacgg atttaatcat gagttggttg aggtcaatca agttttccca
      121 gaggggcgat atgtaactac ggttaaactt tcgaaaagtg gcgacatagt gtttgcgggc
      181 gacagctcgg ggtatgtatt catcctcgaa actctatcga cgccagccct tgcgacatta
      241 tttgaaatac cggtgccaaa agtagtggcg gctgaaggaa tggccgggcg agaagtggtc
      301 gctattgagc ttgatccgga atccgaaccc aaatggatgc gagtgacata tcgctccggg
      361 catgttcatt tgctggagtt acttggtgaa agtaacgcag atctcaatta aaaaaacagc
      421 gaacgaattg gcacgttgaa atgcagaaga aaatttgtat cgtaattccc gcgagattag
      481 ggagtacccg attacccgaa aaggcgcttg ccgatattca cggcaaaccg atgatcgtta
      541 gggtacttga acgagctcgt caaattaacg gagttcacga aattatcgtg gctacagacc
      601 acgagcgaat tgtggctgcg gtcgaagcgg caggcgggac tgcagtcatg acaccggatt
      661 cgttgcagtc gggaactgat cgagttgcct ttgtggccaa tgaacgcaca gcggacattt
      721 ttgtaaacgt tcagggtgat gaacctctgc tcgaccccaa ggccgttgag cgtacgctcg
      781 ctcttataga ggggggccga ttcacaatgg ctactcccgc ggcccctatt cggcaccggg
      841 acgacttgaa gaattcaaat attgtaaagg ttctggtcgc gtctgatgga cgcgcagcgt
      901 atttttcacg gtttccgatt ccgtattcac gtggggatat tccagctgat ttgggggctt
      961 tggcgccgct acaacatatt ggagtttacg cttatacccg cgaaaccctt ttgaaattct
     1021 ggaaactcga gcgaacaact tgggaagtcg cagagtccct cgagcagctt cgcgcgctgt
     1081 atcatggcat tccgatcggt gtggccttga ccgaaaaagc atcaactgga gtcgatacga
     1141 tcgaagacct tgagcgcgta cgttccttga tggtatgaat gtggaatggc ggcaaaaccc
     1201 aagcaaaaaa aatacgtttt cgtaactggc ggcgttcttt cttccattgg caaggggatt
     1261 gcggcggcta gtattggtta tttattagaa aatcgtggcg tgaaggtcac gatgatcaaa
     1321 atggacccgt acatcaacgt agatcctggc accatgagcc cctttcaaca cggggaagtt
     1381 ttcgtgcttg aggatggcgc tgagtgcgac ttggatttgg gccactacga acgatttgtg
     1441 aattgcacgc tcacccgcga aaataatttt actacgggta aggtatacga cactgttatt
     1501 caaaaagagc gccgtggtga gtaccttggt aagacagtac aggtaattcc gcatatcacc
     1561 gaccaaatta aagaaaacgt acgtaaggcc gctgccgacg ccgatttagc aatcgtagaa
     1621 attggtggaa ccgtcggcga tatcgagagt ttgccttttc tcgaggccat tcgacaattt
     1681 aaagcagaag ttggggctga aaacgtattg tatgtgcatg tgactttagt tccgcacatt
     1741 gccgccgcga gcgaacttaa aaccaaaccc acccagcatt cggtaaaaga actacgggaa
     1801 attggaattc aacccgatat attgttgtgt cgatccgatc gcgcacttcc taaagacgtt
     1861 aaagataaaa tcgcgctgtt ctgcaacgtc acgccggatt gtgttttttc tgcgatggac
     1921 gttcaaacca tttacgaagt gc
//
LOCUS       DAOMXO010000479         1939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_34747_length_1939_cov_4.421975, whole genome shotgun sequence.
ACCESSION   DAOMXO010000479 DAOMXO010000000
VERSION     DAOMXO010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34747_length_1939_cov_4.421975"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLH57_09360"
     CDS             <1..783
                     /locus_tag="PLH57_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02861.1"
                     /translation="ASRKLSAVFLKLIPTFDLLLFFFVALTVCQFYSIAYGQTPPSRT
                     LSTGRPSSANSTLPATTMPVPEPTAVETRNKSKWFLKTDYLGVLASHSASGVTLSAGR
                     LLKHTWLDAWLLYTSGNYGAFNINQSSATVNVQEGSASYDREVERYRAATGRVNLFSA
                     GPGLGMMFKLFESERWVEWGHFGLGYARFSDGANSLEFKGALARMQGGVGYRLDPVML
                     SLGLSWNLSYVQRISSPYEMPHDNFLPLQWWGVHVGFSYWIR"
     gene            793..1242
                     /locus_tag="PLH57_09365"
     CDS             793..1242
                     /locus_tag="PLH57_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRK02862.1"
                     /translation="MFLKNRRAIKNFLIFSDFQARYIFWMVLSGILLVGINTGIFYSF
                     MSENYAILVDLSPMTQEAKDLMYAELNKIVWLLLGSSVGVLALLAILALVFSHRAAGP
                     MFNFMKTFEAIKKGDRNVRVRLRPNDHFQEVAREFNEMMDSITSPKS"
     gene            complement(1253..>1939)
                     /locus_tag="PLH57_09370"
     CDS             complement(1253..>1939)
                     /locus_tag="PLH57_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02863.1"
                     /translation="VGIVASIRTNRSNLGRILLYFGLTVGGPVAALVVGKYAYFPRQV
                     VAATTGFLAICALGLLFIIDQARHNRKALPITSLVILLTAWFAGARPWVRSTVFKMPP
                     FPDVAFHQYRDVVATFGPQSNSGDNILIIDGAGTYQFYLKRIYGPASNTEHDVMFKQL
                     KLRRDCWKNHFCVLTYYDSNLTWTTDFSNPPQLIQDLINAPEFGFIKVVGPWVDYRPN
                     NPSVKMLRDW"
BASE COUNT          483 a          429 c          496 g          531 t
ORIGIN      
        1 gcctcccgta aactgtccgc tgttttcctg aagttgatcc ccacctttga cctcctattg
       61 tttttcttcg tcgcgctcac ggtgtgccag ttctattcga ttgcttacgg gcaaacgcct
      121 ccgtcacgta cactttcaac tgggcgtccg agttcggcga attctacgtt acctgcgacg
      181 actatgccgg tgcccgaacc cacggccgtg gaaacccgca ataagtccaa atggttttta
      241 aaaacggatt accttggcgt gctcgcgagt cacagcgcta gtggagtgac tctgtcggcc
      301 gggcgcttgc ttaaacacac ctggctcgac gcctggttgc tttatacgtc tggtaattac
      361 ggcgcattta atatcaatca gtccagtgcc acggttaatg tgcaggaagg aagtgcgtct
      421 tacgatcgcg aagtcgagcg ttatcgtgct gcaacgggtc gagtaaattt attttctgct
      481 gggcccggtt tgggaatgat gtttaaattg ttcgagagtg agcgttgggt ggagtggggc
      541 cattttggtc tcgggtatgc tcgattttca gatggtgcca attcgcttga gttcaaaggt
      601 gctttggcgc gcatgcaagg tggagtggga tatcgcttag atccggtgat gctgagtcta
      661 ggtttgagtt ggaacctatc ctacgtgcag cggatatcga gtccctacga aatgcctcat
      721 gacaattttt tgcctctcca gtggtggggc gtgcacgttg gtttttcata ttggattcgt
      781 taaggagaaa aaatgtttct taaaaatcgc cgagcgatca aaaattttct aatattcagt
      841 gactttcagg cgcgctacat tttttggatg gttctctccg gtatcttgtt ggtcggaatc
      901 aataccggaa ttttttacag ttttatgtca gaaaactacg ccattttggt agatctttcg
      961 ccaatgactc aggaagcaaa agacttgatg tacgccgaat taaataaaat tgtatggctc
     1021 ctgcttgggt cgagtgtggg agttctggct ttattggcta ttctggcact cgtattttcc
     1081 caccgcgcgg cgggtccaat gttcaatttt atgaaaacat ttgaagccat aaaaaaaggt
     1141 gatcgaaatg taagagtacg attgcgaccg aacgatcatt ttcaagaagt agcccgcgag
     1201 ttcaacgaaa tgatggactc cattacgagt ccaaaatcct aagtagagag ttttaccaat
     1261 cgcgcaacat ttttacgcta gggttattgg gtcggtagtc aacccatggc ccaacaacct
     1321 tgataaatcc aaactccgga gcattgatta gatcttgaat aagctgtgga ggattagaaa
     1381 aatcagtcgt ccaggttaaa ttcgaatcgt aataggtcag tacgcaaaag tggtttttcc
     1441 agcaatctct tctaagtttt aactgcttaa acatcacgtc gtgttcggtg ttcgatgcgg
     1501 gtccatagat gcgttttaag tagaactggt aggtgcctgc tccgtcgatg attaagatgt
     1561 tatcaccaga atttgactgc gggccgaaag tggccacgac gtctcggtac tgatgaaagg
     1621 caacgtccgg aaagggcggc attttaaaaa cggtagaccg cacccagggc cgagctcctg
     1681 cgaaccaagc ggtcaaaaga atcaccaggc tcgtgattgg tagggctttc cgattgtggc
     1741 gcgcttgatc gatgataaac aacaaaccaa gggcgcaaat tgcgagaaat ccggtcgtag
     1801 cggccactac ttgtcgcggg aaataagcgt attttccaac tacgagagcg gccactggtc
     1861 caccaaccgt aagtccgaaa taaagcaaaa ttcggccgag gtttgaacga tttgtgcgaa
     1921 tcgaagcaac gatgccaac
//
LOCUS       DAOMXO010000480         1927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35067_length_1927_cov_4.115919, whole genome shotgun sequence.
ACCESSION   DAOMXO010000480 DAOMXO010000000
VERSION     DAOMXO010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35067_length_1927_cov_4.115919"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLH57_09375"
     CDS             <1..546
                     /locus_tag="PLH57_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02864.1"
                     /translation="YACQGGVDFPNLIAAIKDSPADIWSPLVKANYSDLGDGKAIALL
                     TAASIIRQTAEIAGSFEASARTEDANLYMIVVQVQLISTTISGLGQADRTTGNEGIAG
                     WPATSGDMYRCRIQSAVSTIQDSLEYVESSTNLQTLSNAITTLCVGSCSTNRDPEGCS
                     AADNLEGLALMNGISADWTGP"
     gene            593..1711
                     /locus_tag="PLH57_09380"
     CDS             593..1711
                     /locus_tag="PLH57_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02865.1"
                     /translation="MLESVRGSARELPEFYRGIRAMGMGNAYTAVANDEDSVFYNPAG
                     LAQIRRMRFNFINPKLEASKDDYSSVQDIGDAAEQFDASAVSKLFGKNIYANGSMFPN
                     IAFPHFLLGFIYQGEFHLQARNKAMPRVETRYLLDRGLVGGFAFELRGLSRRHFLRLG
                     TSVKWITRRGFDTTIPLAKLMAADKSYFKSLLKGPASGVGLNVGLQYDIPIALTDDLI
                     IGSAWQDVGDTDFASRVNGDGPPKIRNNLSAGLGWIHRFSKKSTAMQNIKVTAEARHL
                     TEENRDPRLKAHLGMELDLDSIFLRFGMNDLAYTAGVGLDMWLIRVAAATYAVETQTL
                     AGMDKERRFALQLEFGFDLARKTSRTGRQEDRYRRPRQ"
     gene            complement(1725..>1927)
                     /locus_tag="PLH57_09385"
     CDS             complement(1725..>1927)
                     /locus_tag="PLH57_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02866.1"
                     /translation="AGASQNLAMQAAQNFAVNEKEAAKNRPDDDGKEYWPYEEEVWED
                     ELNNFRSKIVDGCAKNRKPAAS"
BASE COUNT          475 a          446 c          511 g          495 t
ORIGIN      
        1 tatgcgtgcc agggcggtgt agattttcca aacttaattg ccgcgattaa agactccccc
       61 gcggatattt ggagtccact cgtaaaggct aactactcgg atttaggaga tggaaaagca
      121 atcgcgttat taaccgcagc aagtatcata cgtcaaaccg cggaaatagc tgggtctttc
      181 gaagcgtctg ctcgtacgga ggacgccaat ctttatatga ttgtcgttca agtgcagttg
      241 atttctacga ccattagcgg tctcggtcag gctgatcgaa ctaccggcaa cgaaggcatc
      301 gcgggctggc ctgcgacatc aggagatatg taccgctgtc gtatacagtc ggcggtttcg
      361 accattcagg actcgcttga atacgtggaa tcatcgacga atctccaaac cctctccaat
      421 gcgattacga ctttgtgtgt cggcagttgc tcaactaacc gcgacccgga agggtgtagt
      481 gccgcagaca atttagaggg gctggcactg atgaacggga tctcagccga ttggacggga
      541 ccttaaactg aaaagacaac tgtttttcac tatcgctgtg ctgattgtgg gaatgctaga
      601 gagtgttcgg ggaagcgctc gcgaacttcc cgaattctac cgcggaattc gggcaatggg
      661 catggggaat gcctatacag ctgtggccaa cgatgaggat tcggtattct ataatccggc
      721 gggattggct caaattcgtc gaatgcggtt taatttcata aatccaaaac tagaggccag
      781 taaagacgac tactcttctg tgcaagacat tggagatgcc gccgaacagt tcgatgcttc
      841 ggcagtttca aaattattcg gtaaaaatat ttatgccaat ggttcgatgt ttccgaatat
      901 tgcattccca cattttctct tgggtttcat ttatcaagga gagtttcact tacaggcccg
      961 aaacaaagcc atgccccgag tcgagacacg ctatttgttg gatcgaggat tagtcggagg
     1021 gtttgcgttc gagctgagag ggctttcgcg tcgccatttt ttacggcttg gtaccagcgt
     1081 aaaatggatc acacgtcgcg ggttcgacac cacgattccc ttggctaaac tcatggcggc
     1141 tgacaaaagt tattttaagt cactgctcaa ggggcccgcg agtggggtag ggttgaatgt
     1201 aggactgcag tacgacattc cgattgcgct caccgacgac ttgatcattg gtagcgcgtg
     1261 gcaagatgtg ggggatacag atttcgcatc tcgagtgaac ggcgatgggc ctccaaaaat
     1321 tcgcaataat ctttcggcag ggttagggtg gattcaccga tttagtaaaa aatcgacggc
     1381 catgcaaaat attaaggtta cagccgaagc gcgccaccta actgaagaaa atcgcgatcc
     1441 tagattaaaa gcccaccttg gaatggagct cgacttggat tctattttcc tgcggtttgg
     1501 aatgaatgat ctcgcatata ccgccggagt gggtctcgat atgtggctga ttcgggtagc
     1561 agcggcgacc tacgcggtgg agacccaaac tttggccgga atggataaag aacgtcgctt
     1621 cgccttgcag cttgaatttg gcttcgattt ggcgaggaaa acctcacgta ctggacgaca
     1681 ggaagaccgg taccgacgac cgcgtcagta accgcgatcc aaatttaact cgcagcgggt
     1741 ttacgatttt tggcacaacc atcaacgatc tttgagcgaa agttgttgag ttcgtcttcc
     1801 caaacttctt cttcataggg ccaatattct ttgccatcgt cgtcggggcg attcttagcg
     1861 gcttcttttt catttaccgc aaaattttgc gcggcctgca ttgcgagatt ttgagaggca
     1921 cccgcgt
//
LOCUS       DAOMXO010000481         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35210_length_1921_cov_3.816184, whole genome shotgun sequence.
ACCESSION   DAOMXO010000481 DAOMXO010000000
VERSION     DAOMXO010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35210_length_1921_cov_3.816184"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLH57_09390"
     CDS             complement(<1..858)
                     /locus_tag="PLH57_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRK02867.1"
                     /translation="MSRLIMRAAHRALMAGGFNLRQFKHLGSDVHYIEGGNQSGPLCL
                     VLPGMGDNFSNWTHFLLKYSKKMRLFSLDFPGYTGLSTRATEGDSLTFDEQLRVAEKF
                     ADLITESAKSPIQYVVGNSLGGWQAVKLANRAPNQINNLVLVNPAGYFLSEEEAAKTR
                     DVYEIRNHEDFRRLMKLFWHKVPAYFYPFSLLGLYQFMKGPELTKVVYSVERHHFLNH
                     ELSKIQQPVHLIWGESDGLFPISLANEFRRQVARLDFYPVKKAGHMPQLERPLHFFQI
                     IDTILNRTHS"
     gene            complement(861..1595)
                     /locus_tag="PLH57_09395"
     CDS             complement(861..1595)
                     /locus_tag="PLH57_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRK02868.1"
                     /translation="MQNEARKKLDHLVETTKAPVAIFDLDGTVFDVTYRTMEIVKRFA
                     ATPRWREQYADFIPALEKLRYQDFGYNIETTLNNVGIGKYSERAAHFVHEVETYWFRH
                     FFTDELVLADVAYQGARECVVHLKKLGVHIVYLSGRDIPNMSRGTIESLEKFGFPIKG
                     HGVTLCLKPAYGQDDLLFKKQSIETIGREGTVIATFDNEPANVDMFIKAFPDAVHFHF
                     LSHYARHMELAGPHFYAISSFGEIGF"
BASE COUNT          505 a          426 c          472 g          518 t
ORIGIN      
        1 agaatgcgtt ctatttagga ttgtatcaat aatttgaaaa aaatgaagag ggcgttctaa
       61 ttggggcatg tggcccgctt tttttactgg atagaaatcc aatcgggcca cctggcggcg
      121 aaattcatta gcgagtgaaa taggaaaaag cccgtcactt tcaccccaaa ttaaatggac
      181 gggttgttga atcttcgaca gttcgtgatt caaaaaatgg tggcgctcaa cggagtaaac
      241 gactttggtg agttcgggac ctttcatgaa ttggtaaagt cccaagagcg agaacgggta
      301 aaaataagcg ggaactttgt gccaaaacaa tttcatcaat cgccgaaagt cttcgtggtt
      361 tcggatttca tagacatcgc gggtttttgc ggcttcttct tctgacaaaa agtaaccggc
      421 gggattcact aacactaaat tattgatctg gttcggtgcg cgattggcta gttttacagc
      481 ctgccagccc ccaaggctat tgcccacaac gtattgaatg ggtgatttcg cagattcggt
      541 aattagatct gcaaattttt ctgcaactct caattgttcg tcaaaggtta agctgtcgcc
      601 ttcagtagcg cgggtgctaa ggcccgtata gcccggaaag tccaacgaga agagccgcat
      661 ttttttggaa tatttgagca ggaagtgggt ccagttagaa aaattatcac ccattccggg
      721 caagaccaga caaagcgggc cactttgatt tccgccctcg atgtaatgaa catcgctgcc
      781 tagatgcttg aactgtcgaa gattaaaacc accagccatt agcgctctgt gcgccgcgcg
      841 catgatgagt cgagacatgc tcagaacccg atctctccaa aactacttat ggcgtaaaaa
      901 tgtgggcctg cgagttccat gtgacgagcg tagtgactaa ggaaatgaaa atgaacggca
      961 tccggaaagg ctttgataaa catatcgaca tttgcaggct cgttgtcgaa agttgcaatc
     1021 acggttcctt cgcggccaat ggtttcgatg gactgttttt tgaaaagtaa atcatcttgg
     1081 ccgtaggcgg gtttcaagca aagagtcacg ccgtgacctt taattggaaa accaaatttt
     1141 tcgagcgatt caattgttcc tcgactcata ttgggtatgt ctcgcccaga aagataaacg
     1201 atgtgaacgc cgagtttttt taaatgcacc acgcactctc gggcgccctg gtaggctacg
     1261 tcggccaata ctagttcgtc ggtgaaaaag tggcgaaacc aataggtttc aacttcgtga
     1321 acaaagtgcg cagctctttc ggaatatttt ccaatcccta cgttgtttaa agttgtttcg
     1381 atattatagc caaagtcctg gtaacgaagt ttttctaacg caggtatgaa atcagcgtac
     1441 tgctcgcgcc aacgcggggt agcggcaaag cgtttgacga tctccatggt ccggtaggtg
     1501 acgtcaaaaa cagtcccatc caaatcgaag attgcgaccg gtgccttggt agtctcgacc
     1561 aaatggtcga gtttttttct agcttcattt tgcataagta gggctcctta aagcacggcg
     1621 agcgggcatt tgtccatttc gagttcgatg caatttgcca cgcctaacgc gcgccgtctt
     1681 ttcaaagcgc gcgggtctag gtagccaaat attaagtgtt agctttggaa ggcttcgctc
     1741 atctgtcagg gagggtgcta tgggcaaatg ttcgtacaag gggactggtc ttttcttatt
     1801 ctgggcgttc gtctttgcct ttcccttctt cgcccatgcg gcgccgagcc gaattgaagt
     1861 atttcgcagc gataaactaa aaaccatcgt ctacaaagat aatacggaag ccaacgtatt
     1921 t
//
LOCUS       DAOMXO010000482         1920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35227_length_1920_cov_9.076139, whole genome shotgun sequence.
ACCESSION   DAOMXO010000482 DAOMXO010000000
VERSION     DAOMXO010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35227_length_1920_cov_9.076139"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1017)
                     /locus_tag="PLH57_09400"
     CDS             complement(115..1017)
                     /locus_tag="PLH57_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02869.1"
                     /translation="MSISTSSLEKNIESVSKIVTAASKFGILVGGVCVITYLLRINHF
                     PQDLSVGDGLLFLMAAACFGVIYVFFTASLVSLGITLSPAIRVVFNAFVWGVNIFRKR
                     KAEPAHTLAPFQWSAVLFALYSVVIILALGSQDSTAYWNLPMLSVGLYLFYSVYVSSG
                     NKIKKIEAVKNAVLLTDENENIVQLGNPEKLRRVQLFSLAAILVLPLLIGGVSGQLLD
                     AAMRTAHVRVEKPVIYVKEPYSSLLPKALTSKSRNAPKDYTAFDGTVVLFKGFGKTTV
                     ISFPDGTATRKLEIPNDQLIIENR"
     gene            complement(1124..1786)
                     /locus_tag="PLH57_09405"
     CDS             complement(1124..1786)
                     /locus_tag="PLH57_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02870.1"
                     /translation="MRDKPYSLDLTIPSPMAEIDAKSILETIEIIRTVKSLFVEQNPY
                     LPIYSALGGAFVGAVSSFFPNYIIAKLKDRKEKRSTALQLYAEVKATIELAEHRYYIK
                     QLERVVEAFNSNQITSYSYEIQVSEDRFLIYKSSLDRLGVLEVDIQVKVVEFYQLLEA
                     VVQDVKPGGLLNARPAPVEAYEEALRITKRARELGYEILAIIEKCHRIPNASAGAYNV
                     LG"
BASE COUNT          496 a          467 c          478 g          479 t
ORIGIN      
        1 cgtcatcatt gcgtcgtggg attcactgaa accctcgggt tattcgaggt gccctggaga
       61 tacatctatg acaggcagct tttcggatcg ttcacagcct tttgcatcac gctgctagcg
      121 attttcgatg atgagttggt catttgggat ctcgagcttt cttgttgcag tgccgtctgg
      181 gaaagaaatg acagttgttt ttccaaagcc tttaaagaga actaccgttc catcgaacgc
      241 cgtgtagtcc ttcggtgcgt tccggctctt cgaggtcaga gctttcggaa gtaacgatga
      301 gtaaggctct ttaacgtaga tgacgggctt ttcaacccga acgtgagcgg ttcgcatggc
      361 agcatcgagt aattggccag aaaccccgcc gatgagcaga ggcagcacca aaattgccgc
      421 aaggctaaac aattgtaccc gtctgagctt ttctggattg cccagctgaa caatattctc
      481 gttttcatcg gtgagcaaga cggcgttctt cactgcctca attttcttga tcttgtttcc
      541 actcgatacg tagaccgagt agaacaaata cagaccgact gaaagcatcg gcaagttcca
      601 gtaagcggtt gaatcttgac tgcctagagc cagaatgatg actacggagt acaaagcgaa
      661 tagaaccgct gaccattgaa aaggtgcaag cgtgtgggcg ggttcggctt tgcgtttgcg
      721 gaaaatattg actccccaaa caaaagcatt gaacacgacc cttatggctg gcgacagggt
      781 aattccaagc gaaaccaaac tggcagtgaa gaatacgtaa atcaccccga agcacgcggc
      841 agccatgaga aatagcaaac catcaccgac ggatagatcc tgaggaaagt ggttgattct
      901 cagcaaatag gtgataacgc agacaccacc aacaagaatg ccaaacttcg acgcagcggt
      961 gactatcttc gacaccgact cgatgttttt ctcaagagat gaagtggaga tcgacatgat
     1021 ttatcgctac cgtcgaaggt aaggggcact gctcgacgct ttatcgctca gcgtccagcg
     1081 accgcaggaa gcgatcttga ccgataggtt aggcctcatt gttctagcca agaacattgt
     1141 aagcgccagc ggatgcattc ggaattcgat gacacttctc gatgatggcc aatatttcgt
     1201 aaccgagttc ccgtgctctc tttgtaatgc gtagtgcttc ctcatatgcc tcaaccggtg
     1261 ccggcctcgc gttcaataga ccaccaggct taacgtcttg aactacagcc tccaatagtt
     1321 ggtagaactc aactaccttc acctgaatgt cgacttcgag cacgccaagg cgatctagac
     1381 tactcttgta gatgagaaat ctatcctctg acacttgaat ctcgtatgag tacgatgtta
     1441 tctgattgga attaaacgcc tctaccacgc gctccagttg ttttatgtaa taccggtgtt
     1501 cagcaagttc gatagttgct ttaacttccg catataactg gagagcggta gaacgttttt
     1561 ctttacgatc tttgagcttg gcaattatgt agtttggaaa gaacgacgat actgccccga
     1621 caaaagcgcc accaagggcg ctgtaaatag gaaggtatgg attctgctca acgaacagag
     1681 acttgacggt tcgtattatc tcaatcgttt cgagaataga tttcgcatcg atttcggcca
     1741 tgggagaagg tattgtgagg tctaacgaat agggcttatc cctcacgccg caagcaccgt
     1801 tcagtgtgcg ggcgcctgcc gcgagggata agccttgttg aactgaaccg ccgctatggc
     1861 ggtgacactg gggattctac gcgcggctca tccacattgg atagagcgcg acaggcccct
//
LOCUS       DAOMXO010000483         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35308_length_1917_cov_4.205156, whole genome shotgun sequence.
ACCESSION   DAOMXO010000483 DAOMXO010000000
VERSION     DAOMXO010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35308_length_1917_cov_4.205156"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1917
                     /locus_tag="PLH57_09410"
     CDS             <1..>1917
                     /locus_tag="PLH57_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02871.1"
                     /translation="IALLVYFFWTHFSAIIFVRWVAKKIDAPVEVVSAKWRIRPKYLL
                     QGKIDELSFVLRHRATNTNIAIKTSVRQTLRQGETILSLNPEIKIQHFPPLEGEVEIG
                     LGLKAVRANFKLSCAKPGSITLGKLGQNNLKVAFERCQSTGAVNTTNLLDKTKWQATV
                     INEISNLQTLVKTTKIKINKVTSTHLFYLREAFPLPLYSTAKVLARGIRMELPAYQIE
                     EPLLEINTQSKVTSDSIQLLDVAVSPLNFRAHGGLNFNNTTGELFYHFGDTLDKLVDQ
                     RIVPWFSVEHRQIRRMKARGQLTLSGKFTKVSDDWTHQGTLFLHGKKIEVPNVDLFAE
                     DLVIHVPFTFPSDEDPLWGRFEVRSLEFKSVLLKKLRLFSRITEDGIEITTENSQNKD
                     EPIRQIAWGGKIDIENLYVFRPWSGLIEINAAVFGGPFELAAIQKDLCFASKNPVPGQ
                     LYFEYPSLKQQEHDLTFKGRTRLNLFGGTAELGNIDFSLSGDHPRLRFSFDWNDLDLA
                     AIGSWTKIGDMRGGVEGHLRDVTLILTSLGPIPVSYDLRIRGLNRGGQKIRMYGRAIN
                     NILQLVGMERDDLPWYANVGISASTMWRNLVPATVDYAGFQAKTDGEWTEVSTFDPVG
                     SKKHYFLYGREFTIP"
BASE COUNT          556 a          452 c          412 g          497 t
ORIGIN      
        1 cattgctcta ttggtttatt ttttctggac tcacttcagc gctattattt ttgtgcgatg
       61 ggtcgcaaag aaaatcgatg caccagtaga ggtggtaagc gccaagtggc gaatccgacc
      121 caagtaccta ctccaaggaa aaatcgatga actctcattc gtccttcgtc accgggcgac
      181 taataccaat atcgcaatca aaacatccgt tcgtcagact ctacgccaag gcgaaacgat
      241 actgtcacta aaccccgaaa ttaaaattca gcactttccg cccttggaag gagaagttga
      301 aataggtctt gggctaaaag cagtccgcgc aaactttaag ctctcatgtg ctaaacctgg
      361 aagcattacc ctcggaaaac tcggtcaaaa caatttgaag gttgccttcg aacgctgtca
      421 aagcaccggc gctgtaaata ccaccaattt gctagataaa acgaagtggc aagctactgt
      481 aatcaacgaa atatccaatc tacaaacact ggttaaaact acgaaaatca aaataaataa
      541 agtaacaagc acccatttgt tttacctgcg ggaagcattt ccactaccac tatattccac
      601 tgccaaagtt ctagcgcgcg gtattcgaat ggagttgccg gcctatcaga ttgaagaacc
      661 actattggaa attaatacac aaagcaaagt tacttcagac tcgattcaac tgctggatgt
      721 cgcagtaagt ccacttaact ttcgcgccca cggagggctg aactttaaca atactactgg
      781 cgaacttttc tatcacttcg gggatacact cgataaactg gttgatcaac gaatcgtccc
      841 ttggttcagc gtagagcacc gtcaaattcg caggatgaaa gctcgagggc aattaactct
      901 atccggaaaa tttaccaagg tatccgacga ctggactcat caaggaaccc tttttctcca
      961 tggaaaaaaa atagaagtcc cgaacgttga tctttttgct gaagatttgg taattcatgt
     1021 tccatttact tttccttctg acgaagatcc tctttggggc cgtttcgagg ttcgctctct
     1081 cgaattcaaa tcagtgcttc taaaaaaact acgattattt tctcgaatca ctgaagatgg
     1141 catcgaaatc accactgaaa attctcaaaa taaagacgaa cccatacgac aaattgcatg
     1201 gggcgggaaa attgatatcg aaaatttgta tgtctttcga ccttggagcg gtcttattga
     1261 aatcaacgcc gcagtctttg gcggcccatt tgagctggcc gcgattcaaa aagatctctg
     1321 ttttgcctct aaaaaccccg taccggggca gttatatttc gaataccctt cgctcaaaca
     1381 gcaggagcac gatcttacat tcaaagggcg aactcgactg aacctctttg gcggaacggc
     1441 tgagttgggt aatatcgatt tttcactatc gggcgaccac cctcgcctgc gattttcttt
     1501 cgactggaat gatttagatc tcgcggccat tggtagctgg actaaaatcg gcgatatgcg
     1561 tggcggagta gagggtcacc tacgcgacgt aactttgatt ctaactagtc ttggacccat
     1621 tccagtgtct tacgatctta gaattcgtgg cctaaaccgt ggaggccaga aaattcggat
     1681 gtatggtcgt gcgatcaata acattctgca actcgtcgga atggaacgtg acgatctccc
     1741 ttggtacgcg aacgtgggaa tatctgcgag tacaatgtgg cgcaacctag tgcctgcgac
     1801 cgtagattat gcgggcttcc aagccaaaac cgacggagaa tggaccgagg taagcacttt
     1861 tgacccagtt ggtagtaaaa aacattattt cctttacgga agggagttta caatacc
//
LOCUS       DAOMXO010000484         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35518_length_1909_cov_4.895901, whole genome shotgun sequence.
ACCESSION   DAOMXO010000484 DAOMXO010000000
VERSION     DAOMXO010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35518_length_1909_cov_4.895901"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /locus_tag="PLH57_09415"
     CDS             <1..1711
                     /locus_tag="PLH57_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRK02872.1"
                     /translation="VRANQQIDTFVRAGGLGKDPFAQVFEARRKAFSLFSQQLHDAEI
                     EKMRRYIFNYDRLPTKPGDTPAKTPNLIAGEWVMAAKNEKMLNPGDRRIALAEMPDSG
                     PEEVDRALTYAFEYWKSLKWTDDTLAYRKQVVKNFSRILNYYYEECLTEIRVQIPKTR
                     LEADKDFWEGKRAADHLEGSAERAMLGEIVPTMIEGHTYWKNKNLPAGVSAIFTPMNF
                     IYGIPCIQFIGAYLVGAPFVLKGHPLSAIATTTMMRMLMAAGADPRMVQKIEGFGKGI
                     ESLVHDHRVAIVSVTGSDATAARMQSLRGVRPVRFEGGGCNWSYIDDGYSDEELQKIA
                     IRLTYSKLGFSSHKCTTLHGIAASSAVLSKILPMINREMDEWKLGNPTAVPAEVTKIV
                     GPCMVHKAQTATDIQEAAEKIGAKVYRKGGRVTGGEYAENAEVIAPIILSNATPDMTV
                     KVNWDGRGEKEVNLMTTEFFMPILVTSEMKSFSDFMNFCVNINHHDLAVSIWSRSDVL
                     LSKARKVLGGMVKENDGTDSALEWEEFGASGVGTSGNMGVGDPEATFAIYSRRQKGRH
                     VVF"
     gene            complement(1708..>1909)
                     /locus_tag="PLH57_09420"
     CDS             complement(1708..>1909)
                     /locus_tag="PLH57_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02873.1"
                     /translation="LATKAKTKTKLSYMKGILPYYKQREQGDPHRGVSNPVWQEYGNH
                     RELNRFREQLQIGITEKKACPY"
BASE COUNT          522 a          394 c          487 g          506 t
ORIGIN      
        1 cgttcgagca aaccaacaga tagacacctt tgttcgtgcc ggaggtctag gtaaagaccc
       61 ttttgcgcaa gtcttcgagg cgcgccgcaa ggctttttca cttttttctc agcaactaca
      121 tgacgccgag atcgaaaaaa tgcggcgtta tatattcaat tacgatcgtt taccgaccaa
      181 gccgggagat acaccggcaa aaacacctaa tttaatcgct ggcgaatggg tgatggctgc
      241 taaaaacgaa aaaatgctca atccgggcga tcgccgaata gctttggcag aaatgccaga
      301 ctccggacca gaggaagttg atcgcgcttt gacctacgcg tttgagtatt ggaaaagttt
      361 aaaatggacc gacgatacgt tggcctatcg aaaacaagtc gttaaaaatt tttcaaggat
      421 tttaaactac tattatgaag aatgtttaac ggaaattcgc gttcaaattc cgaaaacgcg
      481 ccttgaggca gataaagatt tttgggaagg caaacgcgcg gccgatcatt tagaaggttc
      541 cgccgagaga gctatgttgg gagagattgt tcccaccatg attgaaggtc acacttactg
      601 gaagaacaaa aatcttcccg cgggagtttc tgcaattttt actccgatga attttattta
      661 cggtattccg tgcattcaat tcatcggcgc atatttagtg ggagcgcctt ttgttttaaa
      721 gggacatccg ttatctgcga tcgcgaccac gacaatgatg cgcatgttaa tggcggcggg
      781 tgctgatccg cgcatggtcc aaaaaatcga aggcttcgga aaaggcattg agtcactcgt
      841 acacgatcat cgtgtagcga tcgtaagcgt gactggttct gacgcgactg ctgcaagaat
      901 gcaaagtctt cgtggcgtgc gtcctgttcg gttcgaaggt ggtggatgta actggagcta
      961 tatcgacgac ggctatagtg acgaagaact gcaaaaaatc gccattcggc tcacttattc
     1021 gaaattgggt ttttccagtc acaaatgcac aacccttcac ggaattgctg cttccagtgc
     1081 ggtgctttca aaaattcttc cgatgattaa tcgcgaaatg gatgaatgga agcttggcaa
     1141 tcccaccgct gtaccggcag aggtaacaaa aattgttgga ccgtgtatgg tgcataaggc
     1201 gcaaacagca acagacattc aagaagcagc cgaaaaaatt ggcgctaaag tgtatcgcaa
     1261 aggtggtcga gttactggtg gtgaatatgc agaaaacgcg gaagtcattg ctcccatcat
     1321 attgagcaat gccacaccag atatgaccgt gaaagttaat tgggatggca gaggagaaaa
     1381 agaggtcaac ttaatgacca ctgaattttt catgccaatt cttgtgacat ctgaaatgaa
     1441 atcgttttct gatttcatga atttttgcgt gaatatcaat caccacgatc ttgccgtgag
     1501 catttggagt cgatcagacg tattgctgtc gaaagctcga aaagtattgg gtggtatggt
     1561 taaggaaaac gacggcactg attctgcgct cgaatgggaa gaattcggag cctcaggcgt
     1621 aggtactagt ggaaacatgg gtgtaggtga tccagaagct acgtttgcaa tatattcccg
     1681 ccgccagaag ggccggcacg tagtgtttta gtaggggcag gctttttttt ctgtgatccc
     1741 gatctgtagc tgttctcgaa atctatttag ttcacgatgg ttgccgtatt cttgccacac
     1801 aggatttgaa accccacggt gagggtcgcc ttgttcgcgt tgtttgtagt agggaagaat
     1861 gcccttcatg tatgaaagtt ttgttttagt tttggctttc gtcgcgagc
//
LOCUS       DAOMXO010000485         1905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35634_length_1905_cov_3.877297, whole genome shotgun sequence.
ACCESSION   DAOMXO010000485 DAOMXO010000000
VERSION     DAOMXO010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35634_length_1905_cov_3.877297"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..>1905
                     /locus_tag="PLH57_09425"
     CDS             58..>1905
                     /locus_tag="PLH57_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRK02874.1"
                     /translation="MAKKITYSNLILFASFFSLFLADTTYNYLLLQKIEHHLVLRRLS
                     EVAYTIFAAGAALTIGILTTRNRPSVGFTILSFGLIALHAIFSHKYILHPFYSRPISP
                     ELFHLVNSTAYSYLLSIAFGLASVLALQVKSVSGFLTLNSFLLLVIGDFAIRFHSAVE
                     NSLVFIWADYAWNVSIAAGAGCALYYVKIRPRNFNLEFTAMKSVRTALALAIFVTNIL
                     LLCGISLLKIYSMPTALEFTSAIFLAFLIWLLSNSISLRVSSVFENVASIVYQQASIT
                     SNSQSLTALVPITKKTNLKETDILVNGYNQLIQHANALNLQIIEATKNRALVEIATQV
                     AHDIRSPLAALDATLPTLTQLESDDTKILLKSATNRIRDIANMLLAKNREIKQLQPSL
                     QNASETRLQPNLIITSLQQILSEKRTQVRNLPGVTIEFSSDAGTQAVFSKFERFAFKR
                     VLSNLIDNAIEAKSQRDLLVEVSLSCSATQAFIAVKDNGRGIPPEVLARLGESGLSFG
                     KVNGNGLGVHHAKATIESWGGSLSFESQEDNGTTTTVTIPLCEKPDIFEIKLKLNPDL
                     YIVIIDDDHAVHELWRQKIASSNVPENHITTFLSADSFYGYFRNGRNKLS"
BASE COUNT          508 a          461 c          363 g          573 t
ORIGIN      
        1 ttcatatttc aacaattttt gagtgaatta ctactggccg ggctaatcgc cgttgaagtg
       61 gcaaaaaaaa taacctattc caacctaatt ctttttgcgt cattcttttc tttatttctc
      121 gcggacacaa cttacaacta cctgctcctt caaaaaattg agcatcatct tgtacttcgt
      181 agactaagcg aggttgcgta tacgatattt gcggcggggg cagcgttaac tattggaata
      241 cttaccacac gaaaccgtcc ctccgtcggt ttcaccattc tctctttcgg tttgatcgct
      301 ctacatgcaa tttttagcca caaatatata ttacatccgt tttacagtcg ccccatttca
      361 ccggaattat ttcacttagt aaacagtacg gcttacagtt atctcctgtc gatcgctttt
      421 gggctcgctt cagtcctagc cctacaggta aaatctgtct ctggtttcct gacactaaat
      481 tcttttcttc ttttggtaat tggtgatttt gctattcgat tccattcggc cgtagaaaat
      541 agcttggttt ttatttgggc ggattatgcc tggaatgtat cgatcgcggc gggagcaggt
      601 tgtgcccttt actacgtaaa aattcgcccg cgtaatttta atttggagtt tacggcgatg
      661 aaaagcgttc gtaccgcttt ggcgctcgca atatttgtaa ccaacatttt gctgctttgc
      721 ggaatatctt tgctcaagat atactccatg ccgaccgcct tagagttcac cagtgctata
      781 tttttagcgt ttctcatctg gttgctatca aattcaattt ctttgagagt ttcttcagtt
      841 tttgaaaatg ttgcaagtat cgtgtaccaa caggcttcca ttacttccaa ctcgcaaagt
      901 ctaaccgcat tggttccgat caccaaaaag acgaatctaa aggaaaccga tatattggta
      961 aacggctaca accaattgat tcagcatgct aacgcgttaa atttacaaat tatagaagcg
     1021 actaagaatc gcgcgctggt ggaaatcgcc acccaagttg ctcacgatat tcgctctccg
     1081 cttgcggcgc tcgacgccac gctccctact cttactcaac ttgaatccga cgatactaaa
     1141 attctattaa aatccgcgac caaccgcatt cgcgatattg cgaacatgct attggccaag
     1201 aacagggaaa taaagcaact ccaaccttct ttacaaaatg cttccgaaac ccggcttcaa
     1261 cccaatttaa ttatcacctc tttgcagcag attctttcgg aaaaaaggac tcaagtccgc
     1321 aaccttcccg gagttacaat agaattttcg tcggacgctg gcactcaagc ggttttttct
     1381 aaattcgagc gttttgcctt taaacgcgtg ctttcaaatt tgatcgacaa cgctatcgag
     1441 gccaaatctc agcgagatct tttagtagaa gtctcgcttt cgtgttccgc cacccaagcg
     1501 tttatagccg taaaagacaa cggtcgaggt attccacccg aggttttggc cagactcggc
     1561 gaaagcggac tttcattcgg caaggttaac ggaaacggcc taggcgtaca tcatgcgaaa
     1621 gcaaccattg agtcttgggg tggaagtctc agttttgagt cgcaagaaga caacggaaca
     1681 accactacag tcactattcc cctctgtgaa aaacccgata tttttgaaat taaattgaaa
     1741 ctaaaccctg atctttacat tgttataatc gacgacgatc acgcggttca cgaactgtgg
     1801 cgccaaaaaa tcgcaagttc taatgttccc gaaaatcaca taactacttt tctttcagcc
     1861 gattcttttt acggctattt tcgtaacggt cggaacaaac tgtct
//
LOCUS       DAOMXO010000486         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35665_length_1904_cov_3.806382, whole genome shotgun sequence.
ACCESSION   DAOMXO010000486 DAOMXO010000000
VERSION     DAOMXO010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35665_length_1904_cov_3.806382"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLH57_09430"
     CDS             <1..366
                     /locus_tag="PLH57_09430"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRK02875.1"
                     /translation="FPMSTQGKQWETSLLVLSREEVKTRFGYVKALKTKVETRFNGVL
                     QQRGDSHIWFSDDERKFVLRFEAKVKIGWVEGIATRVEFGTPPEGEDSATTVVPLLPS
                     DASSGVRKDGFFSRIFRKN"
     gene            370..1470
                     /locus_tag="PLH57_09435"
     CDS             370..1470
                     /locus_tag="PLH57_09435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRK02876.1"
                     /translation="MQIVVIQTAYLGDVILTTPLLVELERLYPGSKIHFVTTVAGSNA
                     LAGFKNVVPYVYEKRKTAFVKGIRELRNRLLIANRNLPFDLVLIAHRSFRSLVLGASI
                     PARRRLSFETFQSRLLKYFLNLETVVYPPYSDAIHYTQRPLEFVRFLSNSTQVPDRRP
                     RLHKNLDTDGSLEKKIGHRLDLPYCVVSPFSTWGTKMWHGDRFAELASIIVKRYSMPV
                     YFCGEPDPKCRVMAEKMFHTFKASLEDAGTENTGANLVGETSVGEMVSLIANAKLLIA
                     NDSAPVHVASSFNVPTVAIFGPTVKRFGFFPLADRQVMVERTNLDCRPCSLHGPELCP
                     KGHFKCMNEIQVEDVFKAVQNVTRGLPWFEQH"
     gene            1452..>1904
                     /locus_tag="PLH57_09440"
     CDS             1452..>1904
                     /locus_tag="PLH57_09440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK02877.1"
                     /translation="MVRTTLKEHLRPLWIILSNRWGGPEQVAVSDLVEMAHAEISMNL
                     ICIEGSPVHAAAMRHPHIKVRAIKEEPRSWFDFSFVRFLKNTIKEFDANLVHVNDESL
                     LWYLYPGLRNLPHVSVVVSRYSTDEASFADIFRRIWLRRIDYVVVLSSH"
BASE COUNT          477 a          423 c          469 g          535 t
ORIGIN      
        1 tttccaatga gcacccaagg taaacagtgg gaaacgtctt tgttggtgtt aagtcgagag
       61 gaagtgaaaa cccgctttgg ttacgtaaaa gcgttaaaga ccaaggtgga aactcgattt
      121 aacggggttt tgcagcaaag gggcgactct catatttggt tttccgacga cgaacgcaag
      181 ttcgtgttgc gtttcgaagc taaggtaaaa attggctggg ttgaaggaat tgcaacgcgc
      241 gtagagtttg gtactccgcc ggagggtgag gattcagcga cgacggtggt gccgcttctg
      301 ccttctgacg ctagtagtgg ggttcggaaa gatggcttct tcagccgaat tttccgtaag
      361 aactaaccca tgcaaatcgt agttattcag actgcctatt tgggtgacgt aattctgaca
      421 accccgttgc ttgtggagtt agagcgcctc taccccggat cgaaaattca ttttgtgacc
      481 accgtggccg gctctaacgc gcttgccggg tttaaaaatg ttgttcccta tgtttacgaa
      541 aaaagaaaaa ccgctttcgt caagggaatt cgcgaattac gcaatcggct acttatagca
      601 aatagaaact taccctttga tctagtgctg attgctcatc gttcttttcg ttctttagtt
      661 ttgggtgcct caattccggc tcggcgccgc ctgtcatttg aaacatttca aagtcgattg
      721 cttaaatatt ttcttaatct tgaaacggtt gtttatccgc cttattcgga cgctattcac
      781 tatactcagc ggccgctgga gttcgtacga ttcttatcga acagcactca ggtgcccgac
      841 cgaagacccc gccttcacaa gaatttagac acagatggct ctttggagaa aaaaattgga
      901 caccgactcg atctgcctta ttgcgtagta agtccttttt cgacgtgggg cacaaaaatg
      961 tggcacggtg atcgcttcgc tgaactcgcc tctattattg tgaagcgcta ttcgatgccg
     1021 gtgtattttt gcggtgagcc ggatccgaaa tgtcgcgtga tggctgagaa aatgtttcac
     1081 acgttcaaag caagcctcga agacgcgggt accgagaata ccggtgccaa tcttgtcggc
     1141 gaaacaagcg ttggtgaaat ggtgtcgcta atcgcaaacg ccaagctgct aattgctaac
     1201 gactctgcac ccgtgcacgt cgcttcttcc tttaatgttc cgacggtcgc aattttcgga
     1261 ccgaccgtta agcgattcgg attttttcct ttagccgacc gtcaagtaat ggtggagcgt
     1321 actaatttag attgtcgccc ctgcagtctt catggacctg aattgtgccc gaaaggacat
     1381 ttcaaatgta tgaacgaaat acaagttgag gatgtcttta aagcggtcca gaatgtcacc
     1441 agagggctgc cttggttcga acaacattaa aagaacatct cagaccgctc tggataattc
     1501 tctcaaatcg ttggggaggc ccggagcaag tagcagtttc agacttggtc gaaatggccc
     1561 acgcggaaat ttcgatgaat ttaatttgta ttgagggaag tccagttcat gccgcagcaa
     1621 tgcgccaccc acacattaaa gtcagagcaa tcaaagaaga accgcgatct tggtttgatt
     1681 tttcgtttgt tcgattttta aaaaatacta ttaaggagtt cgacgccaac ttggtgcacg
     1741 taaatgacga aagccttctg tggtacttgt accctggctt gagaaatttg ccccatgtat
     1801 cagtggtagt gagtcgttac tctaccgacg aagcgagttt tgccgatatt tttagacgaa
     1861 tctggttgcg tcgtatcgac tacgttgtcg tattgagttc tcat
//
LOCUS       DAOMXO010000487         1903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35685_length_1903_cov_4.221861, whole genome shotgun sequence.
ACCESSION   DAOMXO010000487 DAOMXO010000000
VERSION     DAOMXO010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35685_length_1903_cov_4.221861"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLH57_09445"
     CDS             complement(<1..336)
                     /locus_tag="PLH57_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRK02878.1"
                     /translation="MENRTQINGRRTVVTGIGLLSPLGMTVDANWSALVRGQSGIDRI
                     TRFDCSKIPVQIAGEVRGFNPDPFFSSKEARKMGLFMQYGVAASMLALKDAGISGRFG
                     EQGDAAKFDP"
     gene            complement(352..588)
                     /locus_tag="PLH57_09450"
     CDS             complement(352..588)
                     /locus_tag="PLH57_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRK02879.1"
                     /translation="MEDRVRNIICDQLAVEPEKVTLTASFIDDLGADSLDIVELVMTM
                     EEEFDLDIPDEDAEKMKTVGDVIKYIASKSSASN"
     gene            complement(620..1363)
                     /gene="fabG"
                     /locus_tag="PLH57_09455"
     CDS             complement(620..1363)
                     /gene="fabG"
                     /locus_tag="PLH57_09455"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRK02880.1"
                     /translation="MKPQVLITGGSRGIGKAICETLAAGGYEVALTYRSSSSALAVEQ
                     FAKTLSERHNTRVEAFGMDISQTSSIDQAAETILSRFGNLTGIVNNAGLAIDGLFARF
                     RPEQFDEILNTNLRGAYFVTQAFLRPLLKARSGSVVFISSVVGQSGNAGQSAYSAAKA
                     GLLGVAKSLALELASRNIRVNAVTPGFIETDMTGALPEATKQAILPKIPLGAFGSPKD
                     VAGAVAFLLGPDSRYITGQVLAVNGGMYM"
     gene            complement(1360..>1903)
                     /locus_tag="PLH57_09460"
     CDS             complement(1360..>1903)
                     /locus_tag="PLH57_09460"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRK02881.1"
                     /translation="LPRSIKICDCIDFGRENVEAANFNAPGQVVIAGHSRAVQAAIDL
                     TKSDTRFQKAKAIPLQVSAPFHCALMQPARDLMEPLLESAFQAARSVMQFPVLANINA
                     QPHAHTDVWPKLLSEQITRPVLWARSMKTLRELGVERLLEIGPGKVLAGLQKRIDREV
                     EVVNIGTVADLKSFLTGTNK"
BASE COUNT          457 a          498 c          481 g          467 t
ORIGIN      
        1 gggatcaaac ttagcagcgt ctccttgttc tccaaaccgg ccggaaatac cagcgtcttt
       61 caatgcgagc atcgatgccg caacaccgta ttgcatgaag agacccattt tacgggcctc
      121 tttcgaagag aaaaacggat ccggattaaa accacgaact tcacccgcga tttgaacggg
      181 aatcttcgaa cagtcaaaac gcgtgatccg gtcgatgcca ctctggccac gcacaagcgc
      241 agaccagttc gcgtcgaccg tcatgcccaa tggactgagc aggccaatgc cagtgacgac
      301 cgttcgacga ccgttaattt gggtccgatt ctccacgatg agtcggttgt tctagttaga
      361 agcgctggat ttcgacgcga tgtacttaat aacatcgcca accgttttca ttttctcggc
      421 gtcttcgtct gggatgtcga gatcgaattc ctcttccatc gtcatgacaa gttcgacgat
      481 gtcgaggcta tcggcgccaa ggtcgtcaat aaagcttgcc gtgagggtca ctttttcagg
      541 ttctacagct aattggtcac agatgatatt tcttacgcga tcttccacgt ttggtttcct
      601 ttcgtatcaa ttggttccat tacatataca ttccgccgtt aactgccaag acctgaccag
      661 taatgtatcg agagtcaggt ccgagcaaga acgcaacggc gccggcaacg tctttgggac
      721 taccaaaagc accgagtggg attttcggca gaatagcttg tttagtggct tccggcaacg
      781 ccccggtcat gtcagtttcg ataaaccccg gtgttacggc atttacccga atgttgcggc
      841 tggctaactc tagggctagg ctttttgcaa cgcccaacaa accggcctta gcggcagaat
      901 aagctgactg ccctgcattt cccgattgtc caaccaccga gctaataaac accacgctcc
      961 cagacctggc tttaagtaag gggcgcaaaa acgcctgcgt cacgaaatag gctccgcgta
     1021 aattagtgtt tagaatttcg tcgaattgct ctggtcgaaa gcgagcaaac aaaccgtcaa
     1081 tggcgagacc cgcattgttt acgattcctg taagatttcc aaaccgtgaa agaatggttt
     1141 ctgcggcctg gtcgatcgag ctagtttgcg aaatgtccat gccaaaggct tcaacccgcg
     1201 tgttatggcg ctccgagagc gttttggcaa actgttctac tgcaagggct gaggacgacg
     1261 aacgataggt cagcgcgacc tcgtaaccgc cggccgcaag agtctcgcaa atcgctttac
     1321 caatgccacg tgaaccgccc gtgatgagca cttgtggttt cacttgtttg tccctgtaag
     1381 aaaagatttt aagtccgcca cggtaccaat attgacgact tccacttctc ggtcaattcg
     1441 cttttgcagg ccggccaaca ctttacccgg acctatctca aggagtcgtt ccactcccag
     1501 ttcacggagc gttttcatgg aacgagccca gagtacgggc ctggtgattt gttcagataa
     1561 aagtttgggc caaacatcgg tgtgggcatg cggctgggca ttgatgttgg caagaactgg
     1621 aaactgcatg actgaacgcg ccgcctgaaa ggcgctttct agtaaaggtt ccatgagatc
     1681 gcgagccggc tgcatgaggg cgcagtgaaa cggggcgctc acttgaagcg ggattgcttt
     1741 ggctttttga aatcgcgtgt ccgatttggt gagatcaatt gcggcttgaa ccgcacgaga
     1801 gtgacccgcg attacgactt gccccggagc attaaaattt gcggcttcaa cgttttctct
     1861 accgaaatca atgcagtcgc aaattttaat gctccggggc aag
//
LOCUS       DAOMXO010000488         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35815_length_1898_cov_6.963646, whole genome shotgun sequence.
ACCESSION   DAOMXO010000488 DAOMXO010000000
VERSION     DAOMXO010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35815_length_1898_cov_6.963646"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLH57_09465"
     CDS             complement(<1..956)
                     /locus_tag="PLH57_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine monophosphate reductase"
                     /protein_id="HRK02882.1"
                     /translation="MRIRKKTYGFDDLLLVPRFSTIESRSKVDLSLNLGGIALTCPVV
                     SSNMDTITEHEMAIAMHRMGGLGVIHRYLSREARLEQLAKIVDLPYRAMAVGVTLEEV
                     GIAKELMDRGANVLCLDVAHGHSSMMQRVLGELKNYALGLPSGSHTPLRTPIFIAGNV
                     ATPEGASDLERWGADVLKVGIGPGSMCTTRVVTGAGYGQLTALADCAHSVRAPIIADG
                     GIRNSGDATKALAAGASAVMLGKLLAGSKEVPAWARESGTYRGMASAEAQNKHYGYDF
                     KDRHPEGVATRVHSKGPVSEVLGTFLGGVRSGLSYVGARTLT"
     gene            1173..1622
                     /locus_tag="PLH57_09470"
     CDS             1173..1622
                     /locus_tag="PLH57_09470"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRK02883.1"
                     /translation="MTEVVLPSHTNALGTIFGGQIMSWIDIAAAIASSRHARAVCVTA
                     SIDALHFLAPARLGQVVCLFACVNATNRTSMEVGVRVESEDQRTGIRKKIAKAYLTFV
                     AVNEKGQPIEVPPAIPESPDEIRRNREAVLRREARLRLKQQLKNENA"
     gene            complement(1629..>1898)
                     /locus_tag="PLH57_09475"
     CDS             complement(1629..>1898)
                     /locus_tag="PLH57_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02884.1"
                     /translation="NEPNFDNPEFYKIFEKMALMPPGPERRKLICQLEEIAQEEAPWA
                     YGIFENEYRLINKWLKNYYTSEQIMNKYKYLDVDMAAREQFQNEG"
BASE COUNT          475 a          480 c          505 g          438 t
ORIGIN      
        1 tctgttaatg tgcgggcgcc aacgtaggaa agaccagagc gcacaccccc aaggaacgtg
       61 cccaaaactt ccgaaacggg ccctttgcta tggacccgag tggcaacgcc ttccgggtgg
      121 cggtctttaa aatcatagcc gtaatgcttg ttctgagctt cggccgaggc catgccgcgg
      181 taagtgcccg actcgcgagc ccatgctggt acttctttag agccagctaa gagcttgccc
      241 agcatcaccg ctgacgcccc agcagctagc gcctttgttg cgtcgccact attacgaatg
      301 ccgccatccg cgatgatcgg tgcgcgcacc gagtgggcgc aatctgcaag tgctgtaagt
      361 tggccgtagc ccgcgccggt gacgacacgc gtggtgcaca tgcttcccgg gccgatacct
      421 accttcaata cgtcggcacc ccagcgttca aggtccgatg ccccttctgg agtggccaca
      481 ttaccagcaa taaaaattgg agtgcgtagg ggagtgtgtg agccagaggg caggcccaac
      541 gcataatttt tgagttcgcc caacacacgt tgcatcattg aactgtggcc gtgagctacg
      601 tccaagcaga gcacgttagc tccgcgatcc atcaattctt tggcgattcc tacctcttct
      661 agggttacgc ccacggccat ggcgcggtaa ggtagatcaa caatcttcgc cagttgctca
      721 agacgtgctt cacgagaaag atagcggtga atgacgccga gtccacccat gcggtgcatg
      781 gcaatggcca tttcgtgctc ggtgatggtg tccatgttcg aagaaactac agggcaggtt
      841 aatgcgattc cgcccagatt caaagatagg tcgactttgg agcgactttc gatggtcgaa
      901 aatcggggta ctaacaacaa gtcgtcaaag ccataagttt tctttcgaat gcgcatgtaa
      961 aaagaacctc cgatggaatt ccaaaagccc gagcgaccat aacatagtcc gccgaaaatg
     1021 tgctagccgc ggagcaggga aaaaccctat aatggacgaa gttcaaccgg ggaggcttcg
     1081 tggcaaaaaa caaagtgaaa acaaagggtt tggtatcgaa aggcgcaaag agccgactag
     1141 ccggtaagcc cgtgaaagaa tcggtcgttc agatgaccga agtggtgctg cccagtcaca
     1201 ccaatgcttt ggggaccatt ttcggggggc aaatcatgtc gtggatcgac atcgccgcag
     1261 cgattgcttc ttcgcgccat gcccgcgctg tttgcgtaac ggcaagtatc gatgcgcttc
     1321 actttttagc gccggcgcga ctaggtcagg tggtctgtct attcgcttgt gtaaatgcga
     1381 ccaaccgaac ctccatggaa gttggagtta gggtagaaag cgaagatcaa cgcacgggga
     1441 tacgcaaaaa aattgcaaaa gcttatttga cgtttgtagc cgtgaatgaa aaaggccaac
     1501 caatagaggt tccgcccgct ataccggaat cacccgatga aattcggaga aatcgcgaag
     1561 ccgtgcttcg acgtgaagcg cgcctgcgtt taaaacaaca attaaaaaat gaaaacgcct
     1621 agtaggactt atccttcgtt ctgaaattgt tcacgggcag ccatgtcgac atcaaggtac
     1681 ttgtacttat tcatgatctg ctcggaagtg taatagtttt taagccactt gttgatcaag
     1741 cggtattcgt tttcaaaaat accgtaggcc caaggcgctt cttcttgtgc gatttcttct
     1801 aactgacaaa tgagttttct gcgttctggt ccgggtggca tgagcgccat tttttcgaag
     1861 attttgtaaa attctgggtt atcgaaattg ggttcgtt
//
LOCUS       DAOMXO010000489         1897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35851_length_1897_cov_3.991314, whole genome shotgun sequence.
ACCESSION   DAOMXO010000489 DAOMXO010000000
VERSION     DAOMXO010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35851_length_1897_cov_3.991314"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLH57_09480"
     CDS             <1..789
                     /locus_tag="PLH57_09480"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02885.1"
                     /translation="NVHGASSPWGYRTRAQVRGQGRNMGFFEKHSRNLVAIEECPVLH
                     PKIMEQWKRFRKEKAPELAGPNKEFKVEWTLRESGKVTEAVNQPHAALGFTQINSEGN
                     ATLKKIVETAAMRTTTKDLLWDLYGGTGNLVDLLIPSFETTLTVDAENQGVRYSSEQN
                     ETSGHTVVKAKTKTFLQLWSQSKDPKHPNCIVVDPPRTGLEDNAEKIAEVGAERVILV
                     SCDPASLARDLTKFLALYEIRSLDLIDMFPQTYHLESVVELVRR"
     gene            complement(786..1169)
                     /locus_tag="PLH57_09485"
     CDS             complement(786..1169)
                     /locus_tag="PLH57_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HRK02886.1"
                     /translation="MKAPKKALLVYQYPKCSTCKNALKYLEKKKRAAEIRHIVETPPT
                     RAELKKMLEHLDGNLRKMFNTSGLLYKELGLAKKLEKLSENEALDLLSKHGMLVKRPF
                     VLSENRGWVGFKEKDWDQILALSPS"
     gene            1285..>1897
                     /locus_tag="PLH57_09490"
     CDS             1285..>1897
                     /locus_tag="PLH57_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02887.1"
                     /translation="MSAIIRSSRLISAFLLLFASFVSLATLAEPTYAARKKGNCKEIV
                     KQTTFDEGVEEFEVAHPSSKSVSPTIADAYDRELRKTQIASRQSILAKEAELIEIELK
                     TFDQLKNSSLIGGLSEHWITTFEKTPLRGAADEEIEKEVDEDTEMTESTLPSDEATPT
                     AVLAIKSPEQQIAELESILQSEELVHTDARAIFASARIYLKTVQ"
BASE COUNT          528 a          471 c          441 g          457 t
ORIGIN      
        1 aatgtgcatg gggcttcgag cccttggggg tatcgcacgc gagcccaggt acgcggtcaa
       61 ggacgcaaca tgggtttttt cgaaaaacac tcccgtaatc tcgtggctat tgaagagtgc
      121 ccagtcctgc acccaaagat catggagcag tggaaacggt ttcgaaaaga gaaggcccct
      181 gaactagcgg gaccaaataa agagtttaag gtcgaatgga ctctacggga gtccggtaaa
      241 gtcactgaag cagtaaatca acctcatgcg gccttgggat ttactcaaat aaactccgaa
      301 ggcaatgcaa ccctaaaaaa aatagtagaa acggccgcaa tgagaaccac taccaaagac
      361 ttgctttggg atctctacgg aggaaccggg aatttggtcg atttactgat ccctagtttc
      421 gaaacgacgc tcactgtaga cgccgaaaac caaggggtgc gctatagttc cgaacaaaat
      481 gaaacctctg gccacaccgt cgtaaaagcg aagactaaaa cttttcttca gctctggagc
      541 caatcgaaag accccaaaca ccccaactgc attgtagtgg accccccgcg caccggtctt
      601 gaagacaacg ccgaaaaaat cgcggaagtg ggtgctgaac gagtgatctt ggtatcttgc
      661 gaccccgcaa gtcttgctag agacctcacc aagtttcttg ctctatacga gatccgctcg
      721 ctggatctaa tcgacatgtt cccacagacc taccatctgg agagcgtggt agaacttgtg
      781 cgccgttaac tagggctcag agctagaatt tggtcccaat ccttttcttt gaacccgacc
      841 caaccgcggt tttcgctcaa aacgaagggg cgcttaacca gcattccgtg ttttgaaaga
      901 agatccagtg cttcgttttc agagagtttt tctaatttct tcgctagacc cagctctttg
      961 tatagtagcc ccgaagtgtt gaacatcttg cgaagattcc cgtccaggtg ttcgagcatt
     1021 ttcttaagct ctgctcgagt gggaggagtt tctacaatgt gccgtatttc cgcggcccgc
     1081 ttttttttct ctaggtactt cagcgcattt ttgcaggtcg aacacttcgg atattggtaa
     1141 accaataggg cctttttggg tgctttcatg ccgctgtttt agcctaaaat cccagaaaaa
     1201 aagacaaaag attaagaatt tgatgcctcg cacaaattcg ggtaagaggg atcccggtaa
     1261 tcgcttttca gggggtagtc taatatgtcc gctatcattc gctcatctag gttaatttcg
     1321 gcctttttgc tgctattcgc tagttttgtt agccttgcga ccctcgccga accaacttac
     1381 gcagcccgaa aaaagggcaa ctgcaaagaa atcgttaaac aaacgacctt cgatgaaggc
     1441 gtcgaagagt tcgaagtagc ccacccgagt tcgaaaagcg tttcgcccac gattgctgac
     1501 gcctacgata gggaactacg caaaactcag attgcgagtc gtcagagcat tcttgcgaaa
     1561 gaagccgaac taattgagat tgaactaaaa accttcgacc agctaaaaaa ctcaagcctg
     1621 attggcggcc tttcagaaca ctggattacg acctttgaaa aaactccact tcgtggcgcc
     1681 gctgatgaag agattgaaaa agaggtggat gaagatacgg aaatgaccga gtccactctc
     1741 ccttccgacg aagcaactcc cactgctgtt ctggctatta agtcacccga gcaacaaatt
     1801 gccgaacttg aatcgattct tcagtctgaa gaactagtgc acactgatgc tagggcaata
     1861 ttcgcaagcg ctcgaatcta tcttaaaacc gttcaaa
//
LOCUS       DAOMXO010000490         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35897_length_1895_cov_4.925000, whole genome shotgun sequence.
ACCESSION   DAOMXO010000490 DAOMXO010000000
VERSION     DAOMXO010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35897_length_1895_cov_4.925000"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1735)
                     /locus_tag="PLH57_09495"
     CDS             complement(5..1735)
                     /locus_tag="PLH57_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRK02888.1"
                     /translation="METKKDSFCKALFHGDIREDILFPYPAISNDEAESVRMMIDGID
                     KFRPNVKSGQFDRDGAFPDEFIQQVAELGLMGLAIPESYGGLGLSSTGYVRVTEEIST
                     IDGALAVTLGAHQSIGFKALTLFGSDAQKKKYYPGLASGETIACFALTEPGSGSDAGS
                     IKTRAVLSDDGTHYILNGEKIWITNGSMAKFITVFAKTEEPDGQGGVRDKITAFIVDA
                     PAPGITYGPPEDKMGIRASKTTAIHFENVKIPAENVLGGRGKGFKIAMEVLNNGRLGL
                     AGGSLGGCRRMIKTAIEHANTRKQFGKPLNNFGMIQEKIARMVVDTFAAESMIFMTTG
                     LIDRGDCDYSLESAISKVFTTETLWQVSNETLQILGGMGYMRECGVEQVVRDCRINLI
                     FEGANEVLRLFIALSGLQGPGQELAEVARAIKEPLKGIGLLSNIAAKNIRLNVVGVSM
                     LKTHPLLKREAGVLEDYVGEFALQCQKLLQRHGKSIADKQFACKRIADITIDLFAMSC
                     VLARVSSIIETRGADKCEEEIAMAVSFFTVANRRVRGNFKSIDRNDDDQLKLIAAKAC
                     ERGHYGWDVV"
BASE COUNT          449 a          473 c          454 g          519 t
ORIGIN      
        1 aatattaaac aacatcccag ccataatggc cgcgttcgca cgccttggct gcgatcagtt
       61 taagctggtc gtcgtcgttg cggtcgattg atttaaagtt gccgcgaact cggcggttgg
      121 ccactgtaaa gaagctcacc gccatcgcaa tttcttcttc gcatttatcg gcccctcgag
      181 tttcaatgat cgaacttaca cgtgccagta cgcaagacat cgcaaaaagg tcgattgtga
      241 tatccgcaat tcgtttacag gcaaattgct tgtctgctat cgatttacca tgacgttgaa
      301 gtagtttctg acactgcaga gcgaattctc cgacgtagtc ttccagaact cccgcctctc
      361 gtttgagcaa cggatgggtt ttcagcatcg aaaccccaac tacatttagg cgaatatttt
      421 tagcggcaat gttcgagagt agacctattc cctttagagg ttctttaata gctcttgcga
      481 cttcagccag ctcttgccca ggtccctgta ggcctgaaag tgcaataaac aggcgcagaa
      541 cttcgttggc accttcgaaa atcagattaa tacggcagtc ccgcactact tgttctacgc
      601 cgcactcgcg catgtaaccc atgccgccta gaatctggag cgtttcatta gaaacctgcc
      661 aaagcgtttc ggtagtaaat actttggata ttgcggactc caagctgtag tcgcaatcgc
      721 cgcggtcaat gagacctgtg gtcatgaaaa tcatgctctc ggcagcaaat gtgtcgacca
      781 ccatgcgagc gatcttttct tgaatcatgc caaagttgtt cagcggtttg ccaaactgtt
      841 tgcgggtatt tgcgtgctcg atagccgttt tgatcatacg gcggcagccc ccgagacttc
      901 cgccggcgag tccaagacgc ccgttgttta aaacctccat ggcgatctta aaacccttgc
      961 cacggccgcc aagaacattt tctgctggga ttttgacatt ttcgaaatga atcgctgtcg
     1021 ttttacttgc ccgaattccc attttatctt ccggcgggcc ataggtgata cctggggctg
     1081 gagcgtcgac gataaacgct gtaattttgt cgcgcacgcc gccttggccg tctggttctt
     1141 ccgttttggc aaatacagtg atgaatttgg ccatgcttcc gtttgtgatc cagatttttt
     1201 cgccgttgag aatgtagtga gtaccatcat cactcaaaac cgcgcgggtc ttaatgctgc
     1261 cggcatcaga gccggaaccc ggttccgtta gtgcgaaaca tgcaattgtt tcacccgatg
     1321 cgagacctgg gtagtatttc ttcttttgag catctgaacc gaacagtgtg agagctttaa
     1381 aaccaatgga ctggtgcgca ccgagagtga ctgcgagtgc gccgtcgatg gttgaaatct
     1441 cttcggtcac tctgacgtaa ccggtagaac ttaatccgag accgccgtaa ctttctggaa
     1501 ttgccaggcc catgagaccg agttcagcca cctgctgaat gaattcatct ggaaatgctc
     1561 cgtcgcgatc gaattgtccg gactttacat tcggtcgaaa tttgtcgatt ccatcgatca
     1621 tcatgcgaac tgattcggct tcgtcattcg aaatggcagg atacggaaat aaaatgtctt
     1681 cccgaatgtc tccgtggaag agggccttac aaaagctgtc ttttttagtt tccattttgg
     1741 ggttcctacc ttcgatagtg gtgatgacaa attggcagag tatccactag caaacaccga
     1801 catctttgac aaaatgtctt tgcggaaaaa atgatccggt aaaaatggcc ccgttttaag
     1861 caggaaacgg tggtttgaga ggttggcaag ggctt
//
LOCUS       DAOMXO010000491         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35903_length_1895_cov_4.435326, whole genome shotgun sequence.
ACCESSION   DAOMXO010000491 DAOMXO010000000
VERSION     DAOMXO010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35903_length_1895_cov_4.435326"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..885
                     /locus_tag="PLH57_09500"
     CDS             229..885
                     /locus_tag="PLH57_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02889.1"
                     /translation="MVRVAVSALFMMIVLVTSVPVAHAKVARAVQDGFDRVRATCLTE
                     NSLELYQPRFTTLENNNTSGFGSSINTKVLKPSLVGIPYRGRKDATAVAYRELKQEIE
                     AYIAEHELSGFKRACVYQCAANSYFQGGLRGVGFDAFLSVRKNDFKKARGDCVHTSLM
                     AYDLANEHMDIHVTTTSLWYNVDRGSGHAFTVLNVDGAKYVMNNNWGWDEMCVFRAGR
                     "
     gene            complement(1118..1202)
                     /locus_tag="PLH57_09505"
     tRNA            complement(1118..1202)
                     /locus_tag="PLH57_09505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1166..1168),aa:Leu,seq:gag)
     gene            complement(1280..>1895)
                     /locus_tag="PLH57_09510"
     CDS             complement(1280..>1895)
                     /locus_tag="PLH57_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02890.1"
                     /translation="NAWLLKAKGQILAMKYQENDFTYEVSGNGTVLFGIQLHEVMVRS
                     YAPRGTNGHIAAGLGGGGSIGGKDLNLALSLSAGAIALRWTAIPAENYYDDSLITGVG
                     AYVGAVMKVKVYRLKNEMRIAICRTTNFDSIGSDTDWGAVAERLYTNPKKCAVASGEH
                     EFELIKIPFAKIAPELRWRYEDLPREYESPEMVLTVGAKAYIDL"
BASE COUNT          446 a          519 c          451 g          479 t
ORIGIN      
        1 agcaaactgg ggcattaaac gcacgtactt ggccggttat tatagttact ccaaagacgc
       61 agcgaaaaag gattttgacg ccaccgataa cacttggaaa gtcgcactgc gtttcgaatt
      121 ttaaactccc cccacttgcg catctgtttt tattcgcact aacatttttc taacgctctc
      181 attgaaggag cattcgcaaa acatttcgcc acggaaaaag ggggactcat ggttcgagtc
      241 gcagtttcgg cgttgttcat gatgattgtt ttagtgacta gtgtaccggt cgcccacgct
      301 aaagtggcaa gagcggtaca agatggtttc gatcgcgtgc gagcgacttg ccttaccgaa
      361 aactcgctcg aactttacca accgcgtttt acaacgcttg aaaacaacaa cacctcaggg
      421 ttcggcagtt cgattaacac taaagtttta aaaccctctc tggtgggaat tccctaccgc
      481 ggacgcaaag acgccaccgc ggttgcatac cgcgaattga aacaggaaat cgaagcctac
      541 attgcggaac atgagttaag cggattcaaa agagcctgcg tataccaatg cgcagccaac
      601 agctatttcc aaggcgggct tcgcggggtc ggtttcgacg cttttctttc cgtaagaaag
      661 aacgacttca aaaaagcgcg cggcgattgt gtacacacca gtctcatggc ctatgacctt
      721 gccaacgaac acatggacat tcacgtaacc actacgagtc tttggtacaa cgtcgaccga
      781 ggctcgggcc atgcctttac ggtattaaac gtggacggcg ccaaatacgt gatgaacaac
      841 aactggggtt gggacgaaat gtgcgtgttt agggcaggac gctagttcga atcttttttt
      901 atggtgcgct cggacggact cgacctacta cgctcctcgc ctccggctct tcgctgcgta
      961 ggctcacctc gttcgctcag caaaacttcc tgttttgctt ccccgcgcga agtcgctatg
     1021 cgactccccg cgggtcgctc cctcgcgttc gagtccgtcg tcctctgact tcggtcgctg
     1081 tcttcgtttt cgattcttgg atttaacttg ttttttatgg tgcgctcgga cggactcgaa
     1141 ccgtcacggc tttcgccata cgcccctcaa acgtacgtgt ctaccaattc caccacgagc
     1201 gcacttgaga tctacacgag ggtgcccgaa aaattaatcg gcacgaccat gctagtcaag
     1261 gtcgtttttc cgatctcgtt tataaatcga tgtatgcctt ggcacccacg gtcaacacca
     1321 tttcgggcga ttcgtactct cttggaagat cttcgtagcg ccaacgtaac tccggggcaa
     1381 tctttgcaaa cggaattttg atcaactcga actcgtgctc accgctcgca accgcgcatt
     1441 ttttggggtt agtatagagg cgttcggcaa ccgcacccca gtccgtgtcc gagccaatcg
     1501 aatcaaagtt cgtcgttcgg caaatcgcaa tgcgcatttc gttttttagg cggtacacct
     1561 taactttcat gaccgcaccg acgtacgcac ccactcccgt gatcaaactg tcatcataat
     1621 agttttcagc tggaatagca gtccatcgca aagcaatcgc ccctgcgctg agcgaaagtg
     1681 cgaggtttaa gtcttttcca ccaatgctac caccgccgcc taatccagca gcgatatgcc
     1741 cgttggtgcc gcgaggggcg taggatcgaa ccatgacttc gtggagttga ataccgaata
     1801 ggaccgtgcc gttaccggaa acttcgtagg taaagtcgtt ttcctggtat ttcatcgcaa
     1861 gtatctggcc ctttgctttg agcaaccaag cgttg
//
LOCUS       DAOMXO010000492         1894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35922_length_1894_cov_5.523110, whole genome shotgun sequence.
ACCESSION   DAOMXO010000492 DAOMXO010000000
VERSION     DAOMXO010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35922_length_1894_cov_5.523110"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLH57_09515"
     CDS             complement(<1..313)
                     /locus_tag="PLH57_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK02891.1"
                     /translation="MEKSRILVIEDNQDTRQFLETVLSREFEVFSTQNAVAGIEYART
                     KQPDLIILDIMLPGGSGLELCREIRASSALPVIMLTALGADTDRIVGLEIGADDYLVK
                     PF"
     gene            complement(289..675)
                     /locus_tag="PLH57_09520"
     CDS             complement(289..675)
                     /locus_tag="PLH57_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02892.1"
                     /translation="MSDDNKNTPPPPAPPGGENPILAADRQEEERKGLIEAIVKLEQE
                     ITVLEARIRNNSKERADEARQQNDQLKAEFTQLEQELFDRITKQKFPLDAGSISVKLE
                     SVKKAAIDYCTERKLDQELWKNLESL"
     gene            complement(672..1325)
                     /locus_tag="PLH57_09525"
     CDS             complement(672..1325)
                     /locus_tag="PLH57_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02893.1"
                     /translation="MKIKLREQNGISVLEVTGDTTAHDIDVLRAGLKKLLASGKNRLI
                     LDITSADKVTPALVDEIVRFHSLAAEHSGSVVLVGKGDLIREASVRTGSTGIRYFASV
                     DAARGAFDSVETIKVDPRTAYKDLSLDDLKKVHADVQLKNQTLQNKVKDLDGGDYKKL
                     VQENALFARLIPIYNARLAELKKYQTAEPASSPAFQEKIAKVEAYLEDFLKKEGLLT"
     gene            1416..>1894
                     /locus_tag="PLH57_09530"
     CDS             1416..>1894
                     /locus_tag="PLH57_09530"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRK02894.1"
                     /translation="MATKIDSEILKQFQTRFAQVANMPPVVSKVLEEFSDVDIKADRI
                     ANTIRRNQYYEHWLIQEIKALGLKDNVPSLDAAIVLLGMQNVREFICALYLIRAALGR
                     KPTVGKDGKLDPKPKDLLKFAVKTEEYVQARRLQNTDTAFAAGLLFDIVAITAREHF"
BASE COUNT          428 a          445 c          480 g          541 t
ORIGIN      
        1 tgaacggctt caccagatag tcatcggcgc cgatctcgag gccgacgatg cggtcggtgt
       61 ctgcgccaag cgcggtgagc atgatgacgg ggagcgctga gctggcgcgt atctcacggc
      121 aaagttcgag gccgcttcct cccggaagca tgatgtcgag aatgataaga tctggctgct
      181 tcgttcgggc gtattcaatt cccgcgactg cattttgagt agaaaacact tcgaactcgc
      241 gggaaagaac ggtttcaagg aattgacgcg tatcttggtt gtcttcaatt acaaggattc
      301 gagatttttc catagctcct gatctagttt gcgttccgtg caatagtcaa tagcggcctt
      361 tttgacactt tcgagcttaa cggatatcga gcccgcgtcc agcggaaact tctgtttcgt
      421 gattctgtcg aacagttcct gctcgagttg ggtgaattcg gctttaagtt ggtcgttttg
      481 ttgacgcgct tcgtctgccc gttctttgga attattccgt atgcgtgctt cgagcacagt
      541 aatttcctgc tctaatttta caatcgcctc gataaggcct tttcgttctt cttcctgccg
      601 atcagccgcg agaatggggt tttcgccgcc agggggcgcc ggcggtggag gggtattttt
      661 attgtcgtca ctcatgtcag tagtccctcc ttctttaaga agtcttctaa ataggcttct
      721 acttttgcaa ttttttcttg aaacgccgga gaagaagcag gctcagcggt ttgatatttt
      781 tttaattctg caagccgggc attgtagatc ggaataagtc gggcaaacag cgcgttctcc
      841 tggacaagtt ttttgtagtc gcctccatcg aggtctttaa ctttgttctg tagcgtctga
      901 ttcttgagct gcacgtcggc gtgaaccttt ttgaggtcat ctagggaaag gtctttatag
      961 gctgttcgtg ggtcgacttt aatagtttct accgaatcaa atgcaccccg ggcggcgtct
     1021 acgctggcaa aataccgaat accagtactg ccggttcgaa cggacgcctc tcggattaaa
     1081 tcgccctttc cgaccaacac tacgctgccg gaatgttcgg cggccagcga gtgaaaacgg
     1141 acgatttcgt cgactagtgc aggcgtgact ttgtcagcag aggttatatc taatatcagt
     1201 cgatttttgc ccgaagctaa aagtttcttc aatccagccc gtaatacatc gatatcgtgg
     1261 gcagtagtgt cgcctgtaac ttctagtacg gaaattccgt tttgttctcg aagttttatt
     1321 ttcatatgcg ttaatctccg acgcggcctc tggtactccc attgtggatt agatcttgac
     1381 ctcaggcaag cttcagcgcc aaactggtta gtatagtggc cactaaaatt gattccgaaa
     1441 ttttgaaaca gtttcaaacc aggtttgcgc aagtcgccaa catgccaccc gttgtttcaa
     1501 aagttctcga agaattttcg gatgtcgata ttaaggcaga ccgaattgcc aatactattc
     1561 gtcgcaatca gtattacgaa cactggttga ttcaagagat taaagccctg gggcttaagg
     1621 acaacgtgcc gagcttagat gcggcgattg tgctgctcgg aatgcaaaat gtccgcgaat
     1681 ttatctgcgc actttatctt atacgggccg cgcttggacg taagcccacg gtgggaaaag
     1741 acggaaaact tgatcctaaa ccaaaggact tgttgaagtt cgcggttaag acggaagagt
     1801 acgtgcaggc gcggcgactt cagaacacag acactgcttt tgccgccgga cttttgtttg
     1861 atatcgtcgc aattaccgcg cgcgagcatt ttaa
//
LOCUS       DAOMXO010000493         1891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_35992_length_1891_cov_5.081155, whole genome shotgun sequence.
ACCESSION   DAOMXO010000493 DAOMXO010000000
VERSION     DAOMXO010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35992_length_1891_cov_5.081155"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..941
                     /locus_tag="PLH57_09535"
     CDS             186..941
                     /locus_tag="PLH57_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02895.1"
                     /translation="MNLSKSFSVLITLLALTASIAQADGIDGFGLVTATDSLEAKDEN
                     REYAVAATGTHAHVADNKYNAVGSIHARLELGNRGLGLTGTIRENRAEYFSKFLTDVG
                     LSPFFSYGPDLSFRTHSNHNDYIDLTLGGTIGLMLESDSCRAAAGVRAGLDWGTLGSN
                     GLRPAVGAMAVAKCPGFLQLATEYNLLIENDKPGSREIAADLTGQVGRVQLGGRYEQV
                     RDSNSNDESNAAEQQIGTEHRFMIQGSVTHDLL"
     gene            1075..1172
                     /gene="ffs"
                     /locus_tag="PLH57_09540"
     ncRNA           1075..1172
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLH57_09540"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1315..>1891
                     /gene="dnaX"
                     /locus_tag="PLH57_09545"
     CDS             1315..>1891
                     /gene="dnaX"
                     /locus_tag="PLH57_09545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRK02896.1"
                     /translation="MSYLGLARKWRPKTFEDLVGQKHIATTLQNAIRQERISQGYLFT
                     GTRGIGKTSSARILAKALRCTAPVGPGVPCSKCTDCVEITEGRSIDVLEIDGASNNGV
                     DAVRELRDAARFLPSSGRYKIYIIDEVHMLTTAAFNALLKTLEEPPAHVVFIFATTDP
                     QKIPATVLSRCQRFDFKRVSQIELQDHLKKIC"
BASE COUNT          478 a          499 c          456 g          458 t
ORIGIN      
        1 tatttatgat cagtagccac tatgttgatt tgtcgaactt tcaaacaggt gactgaatcg
       61 aaagaagcct ttttcggagt tttgtatttg cccacaacca accctgtgaa accaaacgaa
      121 attctccctg gcccggccgt tgcatttact tagctcgaga gacaaaccag aggagaccaa
      181 gataaatgaa cctttcaaaa tctttttcag ttttaattac tttgttagcc ttgaccgcat
      241 ctatcgctca agccgatgga attgacgggt tcggtttagt cacggcgacc gactccttag
      301 aagccaaaga tgaaaacaga gaatacgcgg tcgccgcaac tggcacccac gcgcacgttg
      361 ccgacaataa atacaacgct gttgggtcga tccatgcgcg cctagagctc gggaatcgcg
      421 gccttggttt gaccggtacc attcgagaaa atcgcgcgga gtatttttcc aaatttctaa
      481 ccgacgttgg cttatctcct ttcttttcat atggacctga cctttcgttt agaacccact
      541 ctaatcacaa cgattatatc gaccttacgt tgggcggtac aatcggcctc atgttggaat
      601 ccgactcgtg ccgagctgca gcaggagttc gcgccggtct cgactgggga accctaggtt
      661 ctaacggact gcgtccggct gttggcgcaa tggcagtggc caagtgtcct ggatttttgc
      721 aattagctac cgaatacaat cttttgatcg aaaacgacaa acctggttct cgcgaaatcg
      781 cagctgacct cactggtcaa gtcgggcgag ttcagctcgg gggccggtac gagcaagtgc
      841 gcgacagcaa ctccaacgac gagtcgaacg ccgctgaaca acaaatcggc accgaacacc
      901 gcttcatgat tcagggctct gtgacgcacg accttctgta atctaaaaac cattgcaggc
      961 gggtaatcgt aagcaacatg gtcagtggta gcgccttttc cgccaatctc gcgacttgcg
     1021 aatgatcccc taaagctcta taagatggat tgatacccca ccgtctggtc cggctctctg
     1081 gtcctagaca catgcgaccc gacgaacccc gccaggtccg gaaggaagca acggtactcg
     1141 gcgttcatgg tgatgtaggt cgccggaggg ccggaccaga cggtggagat cctactttcc
     1201 aaaggaaagt aataaatgag tcctcctgga aggaggtcct ctttttttgg gtatccagcg
     1261 aagtagcagg acgctacgaa gcgaacattg ggctttaatt cgaccaaatt atacatgagc
     1321 taccttggac ttgcgcggaa atggcgcccg aaaacctttg aagacctcgt ggggcaaaaa
     1381 cacattgcca ccacacttca aaacgccatt cgccaagaac gcattagcca aggttattta
     1441 ttcactggca ctcgcggcat tggcaaaact tcttcggcgc gcattcttgc caaagcactc
     1501 cgttgcaccg cacctgtcgg ccccggtgtt ccctgcagta aatgcaccga ttgcgtagaa
     1561 atcacagagg gccgttcaat tgacgtactt gaaattgacg gtgcttcaaa taacggcgtc
     1621 gacgcggtac gggagctgcg cgatgcagct aggtttttac cgtctagtgg ccgctacaaa
     1681 atttatatca tcgacgaagt acacatgctc acgaccgccg cgttcaacgc gcttttaaaa
     1741 acgctcgaag aaccgcctgc gcatgtggtg tttatttttg ccactactga tcctcaaaaa
     1801 attccggcca ctgtgctttc acgctgtcag cgttttgatt ttaagcgtgt ttcgcaaatc
     1861 gaactgcagg accatttaaa gaaaatttgt g
//
LOCUS       DAOMXO010000494         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_36018_length_1890_cov_4.860490, whole genome shotgun sequence.
ACCESSION   DAOMXO010000494 DAOMXO010000000
VERSION     DAOMXO010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36018_length_1890_cov_4.860490"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLH57_09550"
     CDS             <1..289
                     /locus_tag="PLH57_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02897.1"
                     /translation="HEYLSSELVWNYKILRPAARPILYHHEPFLCSPPADQQAFSGLV
                     GLASQISANYRIPKDTSDPVFNTWFAPETSRLQVDRKAIVNIMGKMSRESF"
     gene            complement(293..1144)
                     /locus_tag="PLH57_09555"
     CDS             complement(293..1144)
                     /locus_tag="PLH57_09555"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRK02898.1"
                     /translation="MKKKSSHLHIVRENNSNGAETPLALKQPKSRLAPAATSPLGHDH
                     DNDHIDDHEHSIDPIATLRSPEIQATLLNVLNRVLRENDGLKKNYERLLSQTLQALLK
                     CLEERDPYTFGHSMRVMEYSLLIGRGAELTGKDLRTLELAAMFHDLGKVGVADCVLLK
                     PGKLTEEEQIQMQAHPVKSGEILGLIDEFKDSVPGVRHHHERVDGMGYPDGLSGKQIP
                     WASRIILVADTFDAMTSTRPYRKALPVSTAYEELERYAGTQFDAELVKIFLHEHRKLF
                     GTKKQAA"
     gene            1373..>1890
                     /locus_tag="PLH57_09560"
     CDS             1373..>1890
                     /locus_tag="PLH57_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02899.1"
                     /translation="MGRIRVLQYHLLFGCLLFGLLWRSVALADQFITQTAQSARAVGM
                     GDAAINTERGPYSVFYNPANLAAKDTGVHIQLLNFQVESNGGLITQAGRGASTSFNNL
                     SSLYEDLRTNPNTYVNGRVSVFPNITIRNLHLGILYEVNQGAEVQAHDLGLRVKARNR
                     FAPTAALSFRVF"
BASE COUNT          447 a          449 c          464 g          530 t
ORIGIN      
        1 ccacgagtat ttaagctcgg aactggtttg gaattacaaa attctacgtc ccgcggcgcg
       61 accgatttta tatcaccatg aaccgtttct ttgttcgccc cccgctgacc agcaagcgtt
      121 ttcggggcta gtagggctgg cttcacagat ttctgcgaac tatcggatac ccaaagatac
      181 ctcggatcct gtttttaata cttggttcgc cccagagact agtcgtctcc aagtagaccg
      241 caaagcaata gttaatatta tgggtaagat gagccgcgaa agtttttagc gtttaagctg
      301 cttgtttctt ggtgccgaac aacttgcgat gctcatggag aaaaattttt acaagttcgg
      361 catcgaactg tgtaccggcg tagcgttcca gctcttcata ggccgtgctc accggcaacg
      421 ccttgcgata agggcgcgtt gacgtcattg catcgaaggt gtcggcgaca agaatgattc
      481 tagatgccca gggaatctgt ttgcccgata acccgtctgg gtaacccatg ccatctactc
      541 gttcgtgatg gtgccgaacc cccggaaccg aatctttaaa ttcatcgatc agtccgagaa
      601 tttccccgct ttttactggg tgtgcttgca tttgaatttg ctcttcctcg gtgagtttac
      661 caggtttcag caatacgcag tctgcgacgc ccactttacc gagatcatga aacattgctg
      721 cgagttctaa agtgcgtagg tcctttcccg tgagttccgc accacgaccg attagtagtg
      781 aatactccat cactcgcatg ctgtggccga aagtgtaagg gtctcgttct tctagacact
      841 ttagtaaagc ctgcagggtt tgactcaata agcgctcata attttttttg agaccgtcgt
      901 tttcgcgcag cacgcgattg agaacattga gcagtgtcgc ttgaatttcg ggggaacgca
      961 gagtcgcaat tggatcgatc gaatgttcgt ggtcgtcaat gtggtcgtta tcgtggtcgt
     1021 ggccgagggg cgaagtagct gcgggagcca atcgcgattt cggttgtttt agcgcgagcg
     1081 gcgtttcggc accattcgaa ttgttctcac gcacaatatg gagatgactc gattttttct
     1141 tcaccgtaag acaggccccc tcacaaccct cgtatcggtc caacggaccg acacccttaa
     1201 tacaggttat tttacattac tttactgaaa tagacaacta aagtaagggg ctaattagag
     1261 tgtaactatt ggattttaat ggatttatta agcttaagtt gtgttttgaa ttggccgata
     1321 ggttttttga ggaaaacccc caaggtaggg ggaagtttgg aggaccaggg aagtgggtcg
     1381 cattcgagtt ttacaatatc acctattatt cggctgccta ttgttcgggc tactctggcg
     1441 aagcgttgcg ctcgcagatc agttcatcac ccaaacagca caatctgccc gcgccgtcgg
     1501 catgggtgat gctgcgatta atacggaacg tggtccctat agcgtgtttt acaacccagc
     1561 caacttggct gccaaagaca ccggcgtgca cattcagttg cttaattttc aagtagagtc
     1621 caacggaggt ttaatcaccc aagctggtcg tggggcatcg accagcttta acaacttatc
     1681 gagcctttat gaagatttaa gaaccaaccc taatacctac gtcaacgggc gagtttcggt
     1741 ttttccgaat attacgatta ggaatttaca cttaggcatt ctttacgaag tgaaccaagg
     1801 tgccgaagtt caggctcacg atttaggttt gcgcgtgaaa gcccgcaatc ggtttgcacc
     1861 taccgctgca ctttcgtttc gtgtgtttaa
//
LOCUS       DAOMXO010000495         1888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_36088_length_1888_cov_3.581560, whole genome shotgun sequence.
ACCESSION   DAOMXO010000495 DAOMXO010000000
VERSION     DAOMXO010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36088_length_1888_cov_3.581560"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLH57_09565"
     CDS             complement(<1..952)
                     /locus_tag="PLH57_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02900.1"
                     /translation="MGIRIRGAWGLSLAVISLTSSFAYADVKVPRLQLDVDRREAEKM
                     QLGYGMVSSRKETRKFPDLLLAVDVPLPSFVPIRNLKSYTRITGQITYSPETDEARLE
                     LELTPVEKIVDYEDPDNLIDSEATSYLPISLKAKNGEVSVIDAGISAIGWQKRQTSRL
                     KKFSASELDDFVRLAVEAVKVRYVRLNRSGSDDQFLVATPAVVKFNAGASWDSNCSPN
                     KLVFEMGGVIGFDGAFALKNQQRNGLFTGVGNADLFARVRARVESALGEWELGLQTGV
                     LIHSNKNDGSRLSSASFLIQGSSGPIRANPVTAESNWSRRC"
     gene            complement(999..1460)
                     /locus_tag="PLH57_09570"
     CDS             complement(999..1460)
                     /locus_tag="PLH57_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02901.1"
                     /translation="MDLLKDRRADEATKITRAVAEGILAVGKSSGLDALKASAKARGL
                     DVKTSEKFNRSQGSIAGLGVLPDLVKDAFSKPSTLSKEAKIYENRGSFVVAFSPMEYQ
                     PDLAKFDKEKDALLDSLAQKKHQTLVEQWVSDARQNARVKINPILFQQDAS"
     gene            complement(1629..>1888)
                     /locus_tag="PLH57_09575"
     CDS             complement(1629..>1888)
                     /locus_tag="PLH57_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02902.1"
                     /translation="MGIGPVHAIPKLLKANGQQPARFEERIREDLVRGKFAELVRGMA
                     RVSLEEARHEFLSNEDRRSVSYVLVNSSYGRERVTVSPKEA"
BASE COUNT          472 a          508 c          418 g          490 t
ORIGIN      
        1 agcagcgcct gctccagttc gactcggcgg tcaccggatt cgcccgtata ggcccactgg
       61 aaccttgaat caaaaacgac gcagaactaa gacgcgatcc gtcattttta ttactatgaa
      121 ttaatacacc cgtctgcaac ccgagttccc attcaccaag ggccgactcg acgcgcgctc
      181 gaacacgcgc aaacaggtcc gcattaccca ctccagtaaa cagtccgttg cgttgctgat
      241 ttttaagggc aaaggcccca tcgaaaccaa tgactccgcc catttcgaaa actaatttat
      301 ttggactaca attagagtcc cacgaagcac ctgcgttaaa tttcacgacc gctggggttg
      361 cgaccaaaaa ctggtcgtct gacccggacc gattaagtct aacgtatcgc actttaaccg
      421 cttcgacggc caatcgcaca aagtcatcta gctcagatgc cgaaaatttc tttaaacgag
      481 aagtctgtcg tttctgccag ccgatcgcac ttatgcccgc atcgatcacc gaaacttcgc
      541 cgttttttgc tttcaaagaa attggaaggt aactggtcgc ctctgaatcg atgagattat
      601 cgggatcctc ataatctacg attttttcaa cgggagttag ttccagctca agtcgagcct
      661 cgtcagtttc cggagaataa gtaatctggc cggtaattct ggtgtaggac tttaaatttc
      721 taattggaac aaacgacggc aacggaacgt ctaccgccaa gagcaggtcg ggaaattttc
      781 gggtttcctt tctcgaagaa accattccat aacccagttg cattttctcg gcctcgcgcc
      841 gatcgacgtc cagttgcaag cgtggcactt tgacgtctgc atatgcgaac gatgatgtga
      901 gggaaataac cgcaagcgag agcccccacg cccccctaat cctaataccc atgggagcgc
      961 actaacacat tgcactaatt agtcaaacac tgaaccgctt aactggcgtc ttgctgaaaa
     1021 aggatcggat taattttaac tcgagcattt tggcgggcat cgcttaccca ttgttccacc
     1081 agagtctggt gctttttctg agctaacgaa tccagcaagg cgtccttttc tttgtcgaac
     1141 ttggccaggt ccggttggta ttccatcgga gaaaacgcca ccacaaagga gcctcggttt
     1201 tcataaattt tcgcttcttt gctcaacgtc gagggcttgg aaaacgcgtc tttaaccaag
     1261 tcgggcagaa ctccaagtcc tgcaatggaa ccctggctac gattaaattt ctcactggtc
     1321 ttcacatcaa gcccgcgcgc tttggcagat gctttaaggg catccaaacc gctagacttg
     1381 ccgaccgcaa gaatgccttc agcgaccgca cgggttattt tggttgcttc atcggcccgg
     1441 cgatccttta acaaatccac agcaatccta gtcttaactt catcaaaagc taggtattcg
     1501 ggtttagcgg cgggctcacc tgccttgggt ttagctaacg gggctgcact ttttttggtg
     1561 tataaaaact tatattgttc gtaatgctgt ttagtcgagt ttagacccgc ctcggtttta
     1621 agaagagcct atgcttcttt tggagatact gtaacccgtt ctcttccata actggagttt
     1681 acgagaacgt aggacaccga acgtcgatct tcatttgaaa gaaattcgtg tctcgcctcc
     1741 tccaaagaaa ctcgggccat tccccgaact aattccgcaa atttaccgcg aactaagtct
     1801 tcccgaattc gttcttcaaa acgcgccggt tgttgaccgt tggccttgag cagcttggga
     1861 atcgcatgga ctgggccgat acccatct
//
LOCUS       DAOMXO010000496         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_36107_length_1887_cov_4.420852, whole genome shotgun sequence.
ACCESSION   DAOMXO010000496 DAOMXO010000000
VERSION     DAOMXO010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36107_length_1887_cov_4.420852"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1069)
                     /locus_tag="PLH57_09580"
     CDS             complement(71..1069)
                     /locus_tag="PLH57_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HRK02903.1"
                     /translation="MKKKILVTGADGFIGSHLTEALVKEGYDVRAFVLYNSFNSWGWL
                     DTLDRKILDQIDVFSGDIRDPNGVQRASQGRDIVLHLAALIGIPYSYHSPTTYVETNV
                     LGTLNIVQAARDHGIKVIHTSTSEVYGTAKFVPMTEDHPLQGQSPYSASKIGADQIAL
                     SYYQSFGTEVAVLRPFNTYGPRQSARAVIPTIITQLASGKRDLEIGSLTPTRDFTFVE
                     DTVRGFIAAIHSDAAIGRIINIGSNFEVSIEDTVKIISEIMGKTADISQKKDRTRPEK
                     SEVFRLRCDNTLAKATLGWEPKLAGTTGFRRGLEQTVTWFSNPENLKLFKNHIYNI"
     gene            1288..>1887
                     /locus_tag="PLH57_09585"
     CDS             1288..>1887
                     /locus_tag="PLH57_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017817221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HRK02904.1"
                     /translation="MSTRPRITFDEKGRCNACQWSEKKKSFDWSMRQATLEKLLDKYR
                     RDDGEFDVLVPVSGGKDGSYVAHNLKTKYGMNPLALTITPPLSLEVGDQNLKNFIQSG
                     YNHIQINLHPEVTRLISRHGFIEFGFPYYGWAIGVQSAPVMFAHKIGIGLIFYGEDGE
                     VEYGGSTQTAETPTYDVSYMKKVYLEGGHEKVLELLPKSL"
BASE COUNT          530 a          384 c          435 g          538 t
ORIGIN      
        1 ccaatttttg gaaaacaaga ttaaaagagg caaaacactc tacaaaacat cctttttcag
       61 taaaaaatta tcaaatgttg tagatatgat ttttgaagag cttcaaattt tcggggtttg
      121 aaaaccaagt aaccgtttgt tcgagtcccc gtcgaaaccc agttgttcca gctaattttg
      181 gctcccaccc taaagtagct tttgccaatg tattgtcgca cctcaaacga aaaacctcac
      241 ttttttcggg acgagttcga tctttctttt gacttatatc tgcggtttta cccattattt
      301 cagagattat tttgacagtg tcttcgatgg aaacttcaaa gttgctaccg atgttaatga
      361 tgcgtcctat ggcagcatcg gaatgaattg ctgcgataaa gccgcgtact gtatcctcaa
      421 caaacgtgaa gtcgcgagtc ggagttaatg agccgatttc aagatcacgt tttccgctgg
      481 caagttgggt gataatggtt ggaattaccg ccctcgcaga ttgtcttggg ccgtaagtgt
      541 taaaagggcg aagcaccgca acttcggtac cgaacgattg gtaatacgag agtgcgattt
      601 gatcggcgcc aattttactt gcggaataag gcgactgccc ttgtagggga tgatcctcgg
      661 tcataggcac gaacttggca gtgccgtata cctcgctcgt agaagtgtga atcactttga
      721 ttccatggtc ccgggctgct tgtacgatat ttaaggttcc gagaacattt gtttcaacgt
      781 aggttgttgg ggaatggtag gaatatggaa ttcctatcaa tgccgcgagg tgtaacacga
      841 tgtctcgccc ctgagaggcg cgctggacac cgttcggatc tcgaatatcg cctgaaaata
      901 catcgatttg gtcaagaatt ttacgatcca gcgtgtcgag ccatccccag gagttaaacg
      961 agttataaag cacgaatgct cgaacgtcgt aaccctcttt aactagggct tctgttagat
     1021 gggacccaat aaagccgtcc gctcctgtaa cgagtatttt ttttttcacc aatgctccta
     1081 ccgaaaaaga tgtaccatgg ggcctaacga ggcgtcttgt ctcatgtgaa cattgatatt
     1141 gaaaacaatt ttctatcgtt gaacttcgaa agcaggggct atataaatac gctttgatgg
     1201 aagtatcgag ctcaatttag accccatacg catattcgag gatattttct tatgaataag
     1261 gttttttggt gcacaagttg tcttaatatg tcgacgagac cgcggattac atttgacgaa
     1321 aaaggcaggt gtaatgcgtg tcagtggtcg gaaaaaaaga aaagcttcga ttggtcgatg
     1381 agacaagcca ccttagaaaa gcttttggac aagtatcgaa gagatgatgg agagttcgat
     1441 gttttggtgc ctgtaagtgg tggcaaagac ggttcatatg tcgctcacaa tttaaagaca
     1501 aaatatggaa tgaacccgct tgctttaacc attactcccc cgctctctct agaagtcgga
     1561 gatcaaaatc ttaaaaactt catacagtcc ggctataatc acattcaaat taaccttcat
     1621 ccagaggtca ctcgattaat cagccgtcac ggtttcatag agtttggttt tccgtattat
     1681 ggttgggcaa ttggagttca atctgctcct gtaatgtttg cgcataaaat aggaattggt
     1741 ctaatttttt acggagaaga tggtgaagta gagtacggcg gatccacaca gaccgcagaa
     1801 acaccgactt atgacgttag ttatatgaag aaagtctacc ttgagggagg ccacgaaaag
     1861 gttctggagc tactacctaa aagtctt
//
LOCUS       DAOMXO010000497         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_36187_length_1884_cov_6.248770, whole genome shotgun sequence.
ACCESSION   DAOMXO010000497 DAOMXO010000000
VERSION     DAOMXO010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36187_length_1884_cov_6.248770"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLH57_09590"
     CDS             <1..377
                     /locus_tag="PLH57_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02905.1"
                     /translation="NEENLKEHKKKVEFSVKAVILMGYMVKHAALFPPNGEGISEINF
                     AKITEIEKQANDGKEPFRMDEFGELVKAKIATDLAVKDTNTVLTRVNSKNLARMYGIQ
                     KIIKDIEAINEQKREQTARGGR"
     gene            495..1829
                     /locus_tag="PLH57_09595"
                     /pseudo
     CDS             495..1829
                     /locus_tag="PLH57_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
BASE COUNT          502 a          472 c          440 g          470 t
ORIGIN      
        1 tgaacgaaga gaatcttaaa gaacataaaa agaaggtcga gttttcagtt aaggctgtca
       61 tattgatggg ctacatggtt aaacacgctg ctctgtttcc cccaaacggc gaaggaatat
      121 cagaaatcaa cttcgctaag atcactgaaa tcgaaaaaca ggctaacgac ggcaaagaac
      181 cgtttcgtat ggacgaattt ggcgaactcg taaaagccaa gatagctacc gacctggccg
      241 taaaagacac caacactgtg ctaacccgcg tcaactctaa gaatcttgcc cgtatgtacg
      301 gaattcaaaa aatcataaaa gatattgaag cgatcaacga acaaaagcgc gaacaaacgg
      361 cgcgcggcgg cagatagctt ccgtgccact tttgaattct aaaatttaaa atgggcattc
      421 actgacgcaa gtgttaattc cactccgcgg ggtcaacatt gaatcatcca tccgagtcag
      481 tcattcgagc acgcattgca gaaattctca cccagggttt cggtttctcc tcatttcgac
      541 cctatcaaga agaagtttgc ttaaatgccg ccagcggcag agatcttttg ctggttatgc
      601 cgaccggagc cggaaaatct ttatgctatc aaattcccgg attggcccgg cagcaaacca
      661 cgctagttat tagcccccta ctcgcgctaa ttgaagatca ggtggcgcaa ctgcaggcta
      721 aaggttttaa ggcggaacga attcactctg gtcgggaccg cgaaacttcg cgtcaggttt
      781 gtgtacagta cctcgatggt catcttgatt ttttatttgt ggcacctgaa cgcttggctg
      841 tacctggttt tattgaagta cttgcacgtc gaccgcttgg actcattgcg attgacgaag
      901 cacattgcat ttcacagtgg ggtcatgatt tccgtcccga ataccgcatg ctgggacaac
      961 gcctgccggc actcagaccc gcgccgattg ttgcgctgac cgccaccgct actcctcaag
     1021 tacaagatga tatttgccaa cagctggcca ttccgaacgc gactcgattt gtgcatggat
     1081 ttcgccgcac caacatagca atcgaagtgg ctgagctaaa ccccggcgaa cgaatcgaac
     1141 gactccgttc gattcttact ccggaagaac gccgacccgc tatcgtttac gctcctacac
     1201 gaaaacgagc tgaagaaatt gctgaagcac ttaaaagtta tttcgaagtt gccgtgtatc
     1261 acgcgggacg ttcgtctgga gcgcgcgatc gggttcaaac aaaatttatt acaggaaaaa
     1321 ccgatgtcat cgtcgctacg atcgcctttg gcatgggtat cgataaggcc aatgtacgta
     1381 ccgtcgtgca tatggcttta ccggccagtg tggagggtta ctatcaagag attgggcgtg
     1441 ccggacgtga cgggctacct tcccgagctg ttcttcttca ttcgtttatc gatcgtaaaa
     1501 ctcacgagtt ttttttcgaa cgcgactatc cgccgtccgt tgttttgaat aaactcttcg
     1561 atgctactgg tagcgccccc atcgacagcg acgctctacg gcgtaagaca ggcattgatt
     1621 ctgatacttt cgagaaagct ctcgaaaaac tttggattca caaaggcgta cttatcaact
     1681 ccgatcaaca agttactcgc ggcactgatc actggaaaat tccctaccag tcacactgtg
     1741 agcaccgcaa aatgcaactc gaatatatga cccgttttgc aaatggacac tcgtgtcgta
     1801 tggtccaact tgttcgctat ttcggtgact aagaagatgc aggtggggtt tgcggcattt
     1861 gtgatttttg tgcacctgac caga
//
LOCUS       DAOMXO010000498         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_36191_length_1884_cov_5.096774, whole genome shotgun sequence.
ACCESSION   DAOMXO010000498 DAOMXO010000000
VERSION     DAOMXO010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36191_length_1884_cov_5.096774"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1884)
                     /gene="lon"
                     /locus_tag="PLH57_09600"
     CDS             complement(<1..>1884)
                     /gene="lon"
                     /locus_tag="PLH57_09600"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRK02906.1"
                     /translation="MVVPLFVGREKSINALEEAVNKKLDLFLVAQKQAGTVNPNEADI
                     YNVGTIGSILQILKLPDNTVKVLVEGKRRAHILDHVPNDRYMEVLVEEVVEHIDQNVE
                     VEALVRSLKTVFETYVKLNKRIPPEMLMSVSTVEDPSRLADIIVAHLNLKIEDKQEIL
                     EVSDAGKRLEKLLSLMQGEIEILQVERRIRTRVKKQMEKSQKEYYLNEQMQAIQKELG
                     EKDEFKTELNNLEKQIKAKKMSKEGTEKAMREMKKLRMMSPMSAEATVVRNYIDWLVT
                     IPWSESTEDKNDLTLAEKVLEEDHYGLEEVKERILEYLAVRSLNTNAKGSILCFAGPP
                     GVGKTSLARSISKALGRKFVRCSLGGVRDEAEIRGHRRTYVGALPGKVVQSMKKAGTI
                     NPVFLLDEVDKMSMDFRGDPSSALLEVLDPEQNHNFGDHYLEVDYDLSKVMFILTANS
                     LHSIPRPLLDRMEVINISGYTEIEKFNIARKYLVPKAITENGLSTKQIEIGDKTIEGV
                     IRYYVKESGVRNLERVMNTLCRKVARQVVARKQKKKAEKLVKVTAQDLEKYLGPPKYN
                     VTVAEEKNEIGLVNGLAWTEVGGDMLQIEVTTFPGKGKLTITGKLGEVMQESARTALS
                     YIRS"
BASE COUNT          422 a          498 c          417 g          547 t
ORIGIN      
        1 agagcggatg tagctgagcg ccgtccgggc gctctcctgc atcacctcac ccaatttccc
       61 agtgatggtg agtttgccct tacccggaaa ggtggtgact tcgatttgca gcatatcgcc
      121 accgacttca gtccaagcca aaccgttcac cagaccgatt tcgtttttct cttcggctac
      181 ggtgacgttg tacttcggag gcccgaggta tttttcgaga tcctgcgcag taaccttaac
      241 tagtttttcg gccttcttct tttgtttgcg agccactacc tgccttgcga cctttcggca
      301 gagagtattc ataacccgct cgagattccg aactccggac tcctttacgt agtagcgaat
      361 cacaccttca atagtcttat cgccgatttc aatttgtttg gtcgacaaac cattctcggt
      421 aatcgctttc ggtactaagt acttgcgagc aatgttaaac ttctcaattt cggtgtaccc
      481 agaaatgttg atcacttcca tacggtcgag caaagggcga ggaatactgt gcaatgagtt
      541 cgccgtcaga atgaacatga cctttgaaag atcatagtcc acttcaaggt agtgatcccc
      601 gaagttgtgg ttctgttcgg ggtcgagcac ttcgagcaac gcactcgatg ggtcgccacg
      661 aaaatccatc gacattttgt cgacttcatc tagcaagaaa actggattaa tcgttcccgc
      721 tttcttcatc gactgcacga ctttaccggg tagtgcgccg acataggttc ttcgatgacc
      781 tcgaatttca gcttcgtcac gcaccccgcc caaagaacag cgcacgaact tgcgaccaag
      841 cgctttggaa atcgagcgag cgagcgaggt ttttccaaca cccggagggc ccgcgaaaca
      901 caagatcgaa cccttggcat tagtgttcag cgaacgcacg gctaggtatt caagaattcg
      961 ttccttaacc tcttcgagtc cgtaatgatc ttcctctaga accttctcgg caagcgtaag
     1021 atcgtttttg tcttcggtag attcggacca tggaatggtt acgagccagt cgatgtagtt
     1081 gcgcacaacg gtagcctctg ccgacatcgg cgacatcatg cgaagctttt tcatttcgcg
     1141 catggccttt tcagtgccct ctttggacat tttcttggcc tttatctgtt tttcgagatt
     1201 gtttagctcc gttttgaatt cgtctttttc gccaagctct ttctggatcg cctgcatctg
     1261 ttcgttcaga tagtattctt tttgcgactt ctccatctgc ttctttacgc gcgttcgaat
     1321 acggcgctcg acttggagta tttcgatctc gccttgcatg agcgagagca acttttccag
     1381 tcgtttaccg gcgtcggaaa cttccaaaat ctcctgtttg tcttcaatct taagatttaa
     1441 gtgcgccaca atgatgtcgg ccaaacgcga agggtcttcg acggtcgaca cactcatgag
     1501 catttctggt ggaattcgtt tattaagttt cacgtacgtc tcaaaaactg ttttcagaga
     1561 acgaacgagc gcttcaactt ccacattttg gtcaatatgc tctacaacct cttcgaccag
     1621 tacttccatg tagcggtcgt ttggtacgtg atccaaaata tgagcacggc gtttgccttc
     1681 gacaagaacc tttacggtat tgtctgggag cttcaaaatc tgcagaatcg acccgatcgt
     1741 tccaacgtta tatatatctg cctcgttggg attaaccgta ccggcctgtt tctgcgccac
     1801 caaaaataaa tcgagtttct tgttaacagc ctcttccaac gcgtttatgg atttctcgcg
     1861 ccctacgaag agcggaacca ccat
//
LOCUS       DAOMXO010000499         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_36408_length_1877_cov_3.575192, whole genome shotgun sequence.
ACCESSION   DAOMXO010000499 DAOMXO010000000
VERSION     DAOMXO010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36408_length_1877_cov_3.575192"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLH57_09605"
     CDS             <1..276
                     /locus_tag="PLH57_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRK02907.1"
                     /translation="PSNFPRRNRIISGLSDAVLIVQGTKKSGTLHTARYALEQGRCVC
                     VLPGDIFSDLSYVPHKLLREGAFPVVSAQDLDECLDSAKSQLSLELI"
     gene            329..>1877
                     /gene="topA"
                     /locus_tag="PLH57_09610"
     CDS             329..>1877
                     /gene="topA"
                     /locus_tag="PLH57_09610"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRK02908.1"
                     /translation="MAKAATASTKKALIIVESPSKAKTIQKYLGRSYTVKASVGHIKD
                     LPKSKLGVDLEKNFKPDYQVIKGKDKVIEEIKKAAAIVPEIYLAPDPDREGEAIAWHI
                     SEELPKKGKKVYRVLFNAITKPSILEAMKHPQELDESKYNSQQARRILDRLVGYKISP
                     ILWDKVKRGLSAGRVQSVAVRLVVDREEEVKKFVPEEYWTIDAQFEKSGKPFIAKLAK
                     IDGKDPELPNQQTTDGIVKSLQGVPFEVSSVETRERTRKPQAPFITSRLQQEASRKLG
                     FSAKKTMTLAQHLYEGVDLGDLGTHGLITYMRTDSTRIAPEAIVEVRAFIEKEYGKSS
                     LPAEPIVYKTKKSAQDAHEAIRPSSLEFAPSVVESFLDRDEFRLYQLIWNRYVACQMV
                     PAVYDQTTMELSAKSKEGRNALMRVTGSKLKFPGFTAVYDETPDEDRAKSKTGEEEAG
                     EEASQELPDLVEGEKHTAKEFLPVQHFTQPPPRYSDASLIRELEEKGIGRPSTYAAIL
                     SNIQDREY"
BASE COUNT          550 a          429 c          450 g          448 t
ORIGIN      
        1 ccgagcaact ttccccgaag aaatcgcatc atatctggct tgagcgacgc agtattaatt
       61 gttcagggta caaagaaatc aggcactctg cataccgcgc gatacgcctt ggaacagggg
      121 cgttgcgttt gcgtactgcc gggggatatt ttttcagatt tatcctacgt tccccataag
      181 ttgctgcgcg agggggcatt cccagtggta agtgctcaag atctcgacga atgtctcgac
      241 agcgcaaaaa gtcagctaag tctagaactc atttgacaac aagccgctat tgctaaaaat
      301 aaattcagag tatactcaaa cctcacatat ggcaaaagca gcaaccgctt ccactaaaaa
      361 agcgcttatt attgtggagt ctcccagtaa ggcgaagacg atccagaaat atctgggtcg
      421 tagctatact gtgaaggcga gcgttggcca cattaaagat cttccgaagt ccaaacttgg
      481 cgtcgatctc gaaaagaatt ttaaacccga ctaccaggtg attaaaggta aagataaggt
      541 catcgaagaa attaaaaaag ctgcggccat tgttccagaa atatatctcg cgcccgaccc
      601 tgatcgcgag ggagaagcga tcgcctggca tatttcggaa gagcttccga aaaaaggcaa
      661 gaaggtttac cgcgtattat ttaatgctat tacgaagccc tccattttgg aggcaatgaa
      721 gcaccctcaa gaattggatg aatcgaaata taattcccag caggcgcgac gaattttaga
      781 ccgcctggtt ggttataaaa ttagtcccat tctttgggat aaagtaaaac gcggtctttc
      841 cgccggcaga gtgcagtcgg tggcggtacg attggttgtt gatcgcgaag aagaagttaa
      901 aaaatttgtg cctgaagagt actggacgat cgatgcgcag tttgaaaagt cgggcaaacc
      961 tttcattgcg aagttggcga aaatcgatgg taaagatcca gaacttccga atcaacaaac
     1021 taccgatggt attgttaaaa gcctacaagg agtcccgttc gaggttagtt cggttgagac
     1081 tcgcgagcgg actcgcaaac cacaagcgcc atttataacc tcgcggcttc aacaagaggc
     1141 ttcgagaaaa ctgggttttt cggcaaagaa aaccatgacc ttagcgcaac atctctatga
     1201 aggtgtcgat ctcggagacc tcggtactca cggtttgatc acctatatga gaaccgactc
     1261 aactcgaatc gctcccgagg ctatcgtcga agttcgggcc tttattgaaa aagaatacgg
     1321 caaatcctcg ttgcccgcgg aacccatagt ttataaaacc aagaaaagcg cccaagatgc
     1381 ccacgaagcg attcgacctt caagtcttga gtttgcacct agtgttgtag agtcgttttt
     1441 agatcgcgat gaatttcgat tgtaccaact tatttggaat cgatatgtcg cctgccaaat
     1501 ggttccggcg gtttatgatc agaccacgat ggagctttcc gccaaatcca aagaagggcg
     1561 caacgcgttg atgcgcgtaa cgggtagcaa actcaaattt cctggtttta ccgcagttta
     1621 cgatgagacc cctgacgaag accgagccaa gtcaaaaact ggcgaagaag aagctggtga
     1681 agaggcttcg caagagttgc ctgatctcgt cgaaggtgaa aaacacacag ccaaggaatt
     1741 cttgccggtt caacatttta ctcaaccgcc tccgcgatat tctgatgcaa gtttaattcg
     1801 cgaactcgaa gaaaaaggta tcggccgacc gtctacctac gctgcgattt tgtctaatat
     1861 tcaagatcgg gaatatg
//
LOCUS       DAOMXO010000500         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_36550_length_1871_cov_5.216960, whole genome shotgun sequence.
ACCESSION   DAOMXO010000500 DAOMXO010000000
VERSION     DAOMXO010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36550_length_1871_cov_5.216960"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..874)
                     /locus_tag="PLH57_09615"
     CDS             complement(2..874)
                     /locus_tag="PLH57_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRK02909.1"
                     /translation="MTERKAVLICDADPNTRAVMEELLVGPMKNPLIISSGDGVDAST
                     KCARQKFDLLVTDTKLPKINGLKLLDFIAQLPLAVRPKDVLVLSDELKEDYFKENYPS
                     VFFLEKPCANDAINDVISKVVAKQSAQNESAAPRVEFIGPFVDGTVVVMKTLASTEIT
                     KEKLFLRSNDQISGDISAVIAMNCSRFRGSFAIAFSKATFLKVMQKMLGETFEEVTAE
                     ISDGAGEICNQIFGYAKTELNKKGFDLTPALPSVVFGDNHRIKHFCPGPVIAVQFATA
                     DGPFTIETSVALVR"
     gene            complement(877..1224)
                     /locus_tag="PLH57_09620"
                     /pseudo
     CDS             complement(877..1224)
                     /locus_tag="PLH57_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
     gene            complement(1243..>1871)
                     /locus_tag="PLH57_09625"
     CDS             complement(1243..>1871)
                     /locus_tag="PLH57_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRK02910.1"
                     /translation="KAGSIRAYRTVKLVDFDPETKLDFELSLYLPMNNKHIKFNNAGE
                     SLGQERIEKFNKKKINSLYIDQADTQKFYDYTAEYLRKIGNDSALSATERQDKLQASV
                     RSLLSGVLSDSGKEGTFETGKTFIADCDKIVKTYIMGAKTGSVYKQILSTLDESNGAY
                     THTGKVSTLASLFSLGLGIGKPEDLAAAGLLHDMGMANVPIEIQEKPE"
BASE COUNT          451 a          445 c          415 g          560 t
ORIGIN      
        1 atcagcgaac aagagccacc gatgtttcaa tggtgaaagg tccgtcggcc gtggcgaact
       61 gcacagcaat cacagggccg ggacagaaat gtttgatacg gtgattgtcg ccgaatacta
      121 cagacggcaa tgcgggtgtg agatcgaatc cctttttatt taattcggtt tttgcgtacc
      181 cgaagatttg gttgcaaatt tctccggcac catcactaat ttcagcagtt acttcctcaa
      241 atgtttcacc caacattttt tgcattacct ttaaaaaggt cgccttacta aaagcaattg
      301 caaagctacc tctaaatctc gaacagttca ttgcgatcac cgccgaaata tcgccgctaa
      361 tctgatcgtt actacgaagg aaaagttttt ccttggtaat ttccgtcgaa gctaacgttt
      421 tcataaccac caccgtgcca tctacgaagg gcccaataaa ctcgactctg ggtgctgcac
      481 tttcgttctg ggcactttgt ttggctacca ctttactaat cacgtcgttg atcgcgtcgt
      541 tggcgcaggg tttttctaaa aaaaatacac tgggataatt ttctttaaaa taatcttcct
      601 tgagttcgtc cgacaagacc aagacgtctt taggacgtac ggcaagcggg agttgtgcaa
      661 taaaatccaa caatttgaga ccgttgattt ttggcaattt tgtgtcagtc accagtaaat
      721 cgaatttctg ccgcgcacat ttggtagatg catctactcc atctcccgat gaaataatca
      781 acggattctt catgggaccg actagcagct cttccattac cgctcgagta ttagggtctg
      841 catcgcaaat aagcactgcc ttccgttcag tcatgattat tcttccttct caaaaagagc
      901 caacacctgt ttaaccagcg ttgggtcgtg tctcatttta cttggatcac tcactccgtc
      961 tgcaaggagc ttttcgaaaa actgttttgg cgtaactttc ggtttacccg gaaccaccgt
     1021 catagcgtaa tcaagttcgt cggccaggtg gagcagttgc gcctctttgg taatgcgatc
     1081 cccgggcagg gctttgggat aacctcgacc attgaaacat tcgtggtgct gagcaatcgc
     1141 cttagcgatc gtgtcagaca gaatcatctt tcgagtacga ataagatcct gggtgtattc
     1201 gacgtgtttt tcgtactgag cttgttcttc ttttgtacgc tgctattccg gtttctcttg
     1261 aatttcaatg ggcacattgg ccattcccat gtcatgtaac aagcctgcgg ccgcgagatc
     1321 ttcgggtttc ccaataccta ggcccagcga aaatagtgaa gccaaggtgg aaaccttgcc
     1381 ggtgtgggtg taagcgccgt tggactcatc tagcgtgcta agaatttgtt tgtatacact
     1441 cccggttttc gctcccatga tgtaagtctt aacgattttg tcacagtccg cgataaaggt
     1501 cttaccagtt tcgaaagtac cttctttgcc ggaatcgctc agcaccccgc taagtaaact
     1561 tcgaaccgaa gcctgaagtt tatcttggcg ctcagttgcg ctgagggcgg agtcgtttcc
     1621 aatctttcgt aggtactcag cggtataatc gtaaaatttt tgcgtatcgg cctgatcaat
     1681 gtacaaggaa ttgatcttct ttttattgaa cttttcgatt cgttcctggc ctaacgattc
     1741 gccggcattg ttaaatttga tgtgtttgtt attcatcggc aggtaaagac tgagctcgaa
     1801 atccaattta gtttccgggt cgaagtctac cagttttact gtcctatagg cgcgaatgga
     1861 acctgccttg g
//
LOCUS       DAOMXO010000501         1867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_36653_length_1867_cov_4.979581, whole genome shotgun sequence.
ACCESSION   DAOMXO010000501 DAOMXO010000000
VERSION     DAOMXO010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36653_length_1867_cov_4.979581"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLH57_09630"
     CDS             <1..1002
                     /locus_tag="PLH57_09630"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRK02911.1"
                     /translation="MACATSLSTVAEASGWIHRGAVPWAIAGGSESSSNFPVSFPRSF
                     GRVLGNLQFSKTKTAKLKSLTGLRPRDLTPTVPPVAERLTGLTMGEHCELMIKEWKLP
                     RKPQDEWAVMTHARAAAKQDWHSKFLCSAPKSGGKLVDNLIRGDTNLEKISKLKPAFT
                     KDGTITAANASPMTDGGAAVLLADESYAKKMGWPILALIDDMETSAININNEGLLMAP
                     PYAILRLLKRKGLQLADYDLAEIHEAFAAQVLCNLEALQNEEWCNSRVGLPATKVPPY
                     ERINVNGGSIPLGHPFGATGARLVMQLAKQLQDSNLKSGLISVCAAGGLGFVMTLRR"
     gene            1005..>1867
                     /locus_tag="PLH57_09635"
     CDS             1005..>1867
                     /locus_tag="PLH57_09635"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRK02912.1"
                     /translation="MTSIGLTKTATGIAVLTLDNPRSSVNTLSSTTLGDLQAALDEVF
                     RDKKIIGCVITSAKKSTFLAGADLNEIAGVSNSKEAEKFVRFAQDIFQRIADSPIPFV
                     AAIHGAALGGGLELALACHHRVASLSSETVLGLPEVQLGLIPAAGGTQRLYRLIGLKH
                     ALPLILAGTRVRPDKALKLGLVDRVVDPAVVLDTAISLASSKQNITKKRRPTLESWME
                     LPVINKIALAKVKKETLKKTRGHYPAPLAILTCIETGLRHGLRRGLEAEASCFATLAV
                     GQVSKNLIWLF"
BASE COUNT          491 a          467 c          448 g          461 t
ORIGIN      
        1 atggcttgtg caaccagcct ttcaaccgtt gccgaagcat cgggttggat tcatcgaggc
       61 gctgtaccct gggcgatagc tggcggttcc gaaagtagtt ccaattttcc agtgagtttt
      121 cctcgcagct ttggacgcgt attaggtaat cttcaatttt cgaagaccaa aaccgccaag
      181 ttaaaaagcc tgactggcct tcgaccaagg gatcttaccc ctacagtacc tccggtggca
      241 gaacgtctca ccggtctcac tatgggcgag cactgcgaac tcatgattaa ggaatggaag
      301 ctccctcgaa aaccccaaga tgaatgggct gtaatgactc atgctcgcgc tgctgcgaaa
      361 caagactggc attccaagtt tctttgttct gctccgaagt ctggcggtaa actggtcgac
      421 aatttaattc gtggcgacac gaatctcgaa aaaatatcca aacttaagcc cgcgtttacc
      481 aaagacggta cgattactgc tgctaatgca agtccaatga ccgatggcgg cgctgccgtg
      541 ttgctagccg atgagtctta cgccaaaaaa atgggttggc ccatactcgc gctcatcgat
      601 gatatggaaa cttcggcaat caacatcaat aatgaaggcc tgctcatggc tccaccatat
      661 gcaatactgc gccttcttaa acgcaaaggg ctccaactcg cagattatga tcttgccgaa
      721 atccacgaag catttgcggc ccaggttctc tgtaatctcg aagctcttca gaacgaagaa
      781 tggtgtaatt ccagagttgg tttacctgca acgaaggttc cgccatacga acgaattaac
      841 gtgaacggcg gatcaattcc gctcggtcat ccgtttggcg caactggtgc cagactggta
      901 atgcaactcg ccaaacaact ccaagattct aatctaaagt ccggcctcat cagtgtctgt
      961 gcggctgggg ggctcggttt tgtaatgacg ttgcgacgtt gaaaatgact tcaatcgggc
     1021 tgaccaaaac tgccactggt attgccgtac ttaccttgga caatccccgg agttcggtca
     1081 atacgctctc aagtacaaca ctcggtgatt tgcaagcggc gctcgacgaa gtctttcggg
     1141 ataaaaaaat tattgggtgc gtgatcacga gcgcaaagaa aagtactttc ttggccggcg
     1201 ccgatttaaa cgagatcgcc ggagtcagta attccaaaga agcagaaaaa tttgtccgat
     1261 ttgcgcaaga catttttcaa agaatagcgg acagccccat tcccttcgta gcagccattc
     1321 atggagccgc tttgggaggt ggcctagaat tggctctcgc atgtcaccat cgtgttgcaa
     1381 gtttaagttc cgaaacggtc cttggtttgc ccgaagtcca gctcggatta attcccgctg
     1441 ctggcggcac tcagcgtctc taccgattga ttgggcttaa acatgcgttg ccgctaattt
     1501 tagccggtac ccgtgttcgt ccggacaagg ctctaaaact tggtttagta gaccgagtag
     1561 tcgaccctgc ggtcgtatta gacacggcta ttagccttgc gagttcaaaa caaaatataa
     1621 caaaaaaaag acgcccgact ttggaaagtt ggatggaact ccctgtaata aacaaaattg
     1681 ctctcgcgaa ggtcaaaaag gaaactctga aaaaaacgcg aggtcattac cccgcaccac
     1741 tcgctatttt aacttgtatc gaaactggcc ttcgtcatgg tctacgaagg ggactcgaag
     1801 cagaagcaag ctgttttgca actcttgcgg taggccaggt atcgaaaaac ctcatttggc
     1861 tgttttt
//
LOCUS       DAOMXO010000502         1853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37034_length_1853_cov_5.627920, whole genome shotgun sequence.
ACCESSION   DAOMXO010000502 DAOMXO010000000
VERSION     DAOMXO010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37034_length_1853_cov_5.627920"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1309)
                     /locus_tag="PLH57_09640"
     CDS             complement(140..1309)
                     /locus_tag="PLH57_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRK02913.1"
                     /translation="MNRLFAHELFFSDEHKMLQKSVRDFANTVLAPKIEALDEEEKFD
                     RSCFKKLAELGLLGITVPEEYDGTNMGCIAASIAMDEIGAIDASTALTYLAHSMLAVN
                     NLATNGNDEQKKRYLPKLLNGEWIGGMGMTEPGAGSDALAMTTRAVKKGNKYILNGTK
                     MFITNAVVGDFFYVYARTGDGKKDISTFLVETKFPGFKMGKKLKKMGMRASPTGELIF
                     ENCEVPAENLVGREGDSVAHMIKNLDIERITISAISLGIARSSIDHAVRYAKDRTQFG
                     KPISDFQMIQAMLADAQAEYEAAAAYVYGAAKAWDDGILTPQNSRSLGAKVKLVAARM
                     GTKVALDAIQILGGYGYIKEFPVERYMRDAKLMEIGAGTNEILRVLIARDLLGDL"
     gene            1350..>1853
                     /locus_tag="PLH57_09645"
     CDS             1350..>1853
                     /locus_tag="PLH57_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02914.1"
                     /translation="MNESFERINATRQNKTASGLSSAKLKEKDRFAQAFNILVNYWEI
                     KPSLYEAKLQELALRDVRVVSAFLPWAHVETDIYHSLKKFLRAAVAARLRVRLFVMPE
                     LGVNYPNAGVPKELLTNHANLAVNRLGQVIYSFAAPNIFSMPSFASTEVLKRFGNYLL
                     KVGSVLGE"
BASE COUNT          421 a          450 c          457 g          525 t
ORIGIN      
        1 gctcgtagtg catgggtaca gttaatcgca acagcccttg taggtcaaat aggggcttta
       61 atccgcgtat tttcgccttt ttctattaga ggcgattctg aacgtgggta atttccaggt
      121 gactcactgg ggttactacc tagagatccc ccaacaaatc gcgtgcgatc agtactcgaa
      181 gaatttcgtt agttccggcg ccaatttcca tgagcttagc gtcgcgcatg tagcgttcaa
      241 ccgggaattc cttaatgtaa ccgtagccac ctaaaatttg aatggcatcg agcgctactt
      301 tagtgcccat tcgtgcagcg actaatttga ctttggcgcc aagcgagcgg gaattttgcg
      361 gtgtcagtat gccgtcgtcc cacgctttgg ctgctccgta gacgtatgcg gctgcggctt
      421 cgtattcggc ctgggcatcg gccaacatgg cctgaatcat ttgaaaatca gaaatgggtt
      481 ttccgaattg ggtgcggtct ttggcgtagc gaactgcatg atcgatcgac gagcgagcaa
      541 tgcctaaact aattgctgaa atcgtgattc gttctatgtc caggtttttg atcatatgag
      601 caaccgaatc accctcacga cccaccaggt tctcggccgg tacttcgcag ttttcaaata
      661 tcaattcccc agtgggcgag gcgcgcatgc ccattttttt aagttttttg cccattttga
      721 aaccggggaa cttggtctcc accaaaaagg tagaaatgtc tttcttgcca tcaccagtgc
      781 gggcgtacac gtaaaagaaa tcacccacta ccgcgttggt gataaacatt tttgtaccgt
      841 ttaaaatgta tttattgccc ttttttaccg ctcgcgtggt cattgcaagg gcgtcgctgc
      901 cggcgccggg ttcagtcatg cccatgccgc cgatccactc gccgtttagc agttttggta
      961 gatagcgctt cttttgttcg tcgttaccgt tggtggcgag attgtttacc gccaacatgg
     1021 agtgggctag gtaggtgagt gcggtcgagg cgtcgatcgc gcctatttcg tccatggcaa
     1081 tgcttgccgc aatgcatccc atgttcgttc cgtcgtattc ctcaggcacc gtaatgccta
     1141 aaagtccaag ctcagcaagc ttcttgaagc acgaccggtc aaatttttcc tcttcgtcca
     1201 gggcttcaat cttaggtgca agtacggtgt ttgcgaagtc gcgcacgctt ttttgaagca
     1261 ttttgtgctc gtcggaaaaa aataattcgt gtgcaaaaag tcgattcata gctttttcct
     1321 aaataatgaa tatgttacct ttttagacga tgaatgagtc cttcgagcgc atcaatgcca
     1381 cgcgccaaaa taagacggct tccggcctaa gtagtgcaaa gctaaaagaa aaagatcgct
     1441 ttgcgcaagc ttttaatatc ttagtgaact actgggaaat caagccgtca ctttacgaag
     1501 ctaagctcca agagctcgcc ttgcgggacg tgcgagtggt tagcgccttt ttgccatggg
     1561 ctcacgttga aactgacatt tatcactcat taaaaaagtt tttacgtgcg gccgtggcgg
     1621 ctcgtctgcg cgttcgtttg tttgtaatgc ccgagctagg agtgaattat ccgaatgccg
     1681 gcgtacccaa agagctcctc acgaatcatg ccaatctcgc ggtaaatcgc ctagggcaag
     1741 tgatttacag tttcgccgct ccgaatattt tttcgatgcc gagctttgct tcgactgagg
     1801 ttttaaaacg atttggcaac tacctgctca aggtaggttc ggtgctcggt gag
//
LOCUS       DAOMXO010000503         1850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37112_length_1850_cov_3.818942, whole genome shotgun sequence.
ACCESSION   DAOMXO010000503 DAOMXO010000000
VERSION     DAOMXO010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37112_length_1850_cov_3.818942"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLH57_09650"
     CDS             complement(<1..377)
                     /locus_tag="PLH57_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02915.1"
                     /translation="MKGEGGVRVNYLEKLENESKRLMQAGKRKEALEALVAAEKHAVE
                     DRERQRLSGKRRLYAEQFLRTDSFEKYQAAKVAAGSLNGGECLSEIEKIEKGDQDNIQ
                     TIVLKGHCYSLLDKKVEAERLFE"
     gene            complement(442..1464)
                     /locus_tag="PLH57_09655"
     CDS             complement(442..1464)
                     /locus_tag="PLH57_09655"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HRK02916.1"
                     /translation="MSKFSMVDKLNLARTPTPIERLPKLSALASKFAEVEIAVKRDDL
                     THVIATGNKIRKLELHFAAAKKRGARVVFTCGATQSNHARATAVLSRQLGLECVLFLR
                     DEGTSRQLTGNLLLDALFGADIRFITPEQFERIDIVFAEAAEVFKKRDGISPFFIAEG
                     GSDDIGGMGYALAYEEIAGQVPYPFDSIVVANGSGGTQAGLILGKTVFEHENTKIVGL
                     NVCKTAPEMAAKTKKVALSTIQRHRLPISFMPADIHIIDGYVTPGYAKASPQLYNFIV
                     QACRADGILLDPVYTGKALYGLIQELTASRQRAEYFGKKILFVHTGGFPSIFAHENEI
                     EKALHG"
     gene            1485..>1850
                     /locus_tag="PLH57_09660"
     CDS             1485..>1850
                     /locus_tag="PLH57_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02917.1"
                     /translation="MRPITWSTVIKTLTHLKVKNTARFAFAITLAFGLGSFNISTAQS
                     LKTMEENEVGLDAPVDSESSNRLLPAEEETRPIDAEEFRVQIKKKSTSGRVVLLDDPT
                     ENRPKPGKILLLKNGKENVV"
BASE COUNT          486 a          462 c          391 g          511 t
ORIGIN      
        1 aattcgaata ggcgttcggc ttcaactttc ttatctaaaa gcgaataaca atgtcccttt
       61 aaaactatag tttgaatatt gtcctgatca cctttttcga ttttttctat ttctgaaagg
      121 cactcacctc cattgaggga accggcggcc actttggcgg cttgatattt ttcgaaagaa
      181 tcggttcgca gaaattgctc ggcatacaat cttctttttc cgctaagccg ctgtcgttcg
      241 cgatcttcaa cagcgtgttt ttccgcagcc accaacgctt ccaatgcttc ttttcttttt
      301 ccagcctgca ttaggcgttt agattcgttt tctaactttt ctaagtaatt aactcgaact
      361 ccaccctcac ccttcatttg gcggttttcc gcgttcgcaa ccgataaatt cccgaaaagt
      421 aacagtaata aaagttgagc cctaaccatg tagcgctttt tcgatttcgt tttcatgagc
      481 aaatattgaa ggaaacccac cggtatgaac aaaaagtatt tttttcccaa agtattcggc
      541 tctttgtcga gatgcagtta actcctgaat caagccgtac aatgctttac cggtgtaaac
      601 gggatctaaa agtattccat cggctcgaca tgcctgaaca ataaaattat acagctgcgg
      661 ggatgctttc gcgtacccag gagtaacgta accgtcgatg atgtgaatat cagcgggcat
      721 aaaagaaatc ggaaggcgat ggcgttgaat agtgctgagg gccacttttt tagtcttcgc
      781 agccatctcc ggtgcggtct tgcagacatt aagtcccact attttagtgt tttcgtgttc
      841 gaacacggtt ttgcctaaaa taagcccggc ctgagttcct cccgatccgt tcgctacgac
      901 gatcgaatca aatggatacg gcacctgccc ggctatctct tcgtatgcta gcgcataacc
      961 cataccgccg atatcgtcgc ttccaccttc ggcaataaag aacggactga ttccgtcgcg
     1021 ttttttgaaa acctctgccg cttccgcaaa tacgatatcg attctttcga attgctctgg
     1081 agtaatgaaa cggatgtcag ccccaaaaag tgcatcgagc agtaaattgc cagtcagttg
     1141 ccgtgaggtc ccctcgtccc ttaagaatag gacgcattcg agacccaatt gcctacttaa
     1201 aacagccgta gctctggcgt gattactttg agtcgctcca cacgtgaata ccactcgcgc
     1261 tccacgtttt tttgcggcgg caaagtgtag ctctaatttt cgaatcttat tcccggtggc
     1321 gataacgtgg gtcagatcgt cccttttcac agctatttct acctcggcaa attttgacgc
     1381 cagagcagat aacttaggga gacgctcaat gggcgtaggg gttcgggcca gattgagctt
     1441 gtccaccatt gaaaacttag acatacttga ccaagagagt tgatatgcgc cccatcacct
     1501 ggagcactgt gattaaaacg ctgacccatc taaaagtcaa aaatactgcg cgattcgctt
     1561 ttgcgatcac gctagcgttt ggactaggtt ccttcaatat ttccaccgct cagtcactta
     1621 agaccatgga agaaaatgaa gtcggactag atgcccccgt agactcggaa tcgtccaatc
     1681 gactattgcc ggccgaagaa gaaacccgtc cgatcgacgc tgaagaattc cgtgttcaga
     1741 ttaaaaagaa gagtacctcg ggtcgagtag ttctactcga tgatccaacc gaaaatcgac
     1801 ccaaacccgg taaaatccta ttgttaaaaa acggcaaaga aaacgtagtg
//
LOCUS       DAOMXO010000504         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37340_length_1842_cov_3.949077, whole genome shotgun sequence.
ACCESSION   DAOMXO010000504 DAOMXO010000000
VERSION     DAOMXO010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37340_length_1842_cov_3.949077"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLH57_09665"
     CDS             complement(<1..281)
                     /locus_tag="PLH57_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02918.1"
                     /translation="MQVTEKNKSAEKTAPAVMTTRLEILDAGEEVAQSHVLHQGLTLI
                     GQSPSAHIHLNGKGVGGLHLGIKAGKKATDPVTVMDLGGPSQLKLSGKP"
     gene            complement(302..655)
                     /locus_tag="PLH57_09670"
     CDS             complement(302..655)
                     /locus_tag="PLH57_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02919.1"
                     /translation="MSRLKTAVYAGSILMCALLLGRATHAAEFGQDRGDTEVVDTQVT
                     RTTDSKGLTTEKRVVTKVRSRTVVDFEEASIEGKVKKPNAAYLLHGADLEFKHLYRVK
                     HGQKNRILGSYEYVR"
     gene            complement(759..>1842)
                     /locus_tag="PLH57_09675"
     CDS             complement(759..>1842)
                     /locus_tag="PLH57_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02920.1"
                     /translation="FELGNSYEELLKLKLDSKTRSATEQNLKTIRDLNHTKFIQGGAK
                     VVNSDADVEKTWAEFQRFEKSLGDFFKLRFVKSNNNPQSNFKKKADQHKILNATLEKT
                     VDRTPKSLRGAARLWATIVKTELLGEFAETLETAALPAVLNQATEADRKAYVETIMGN
                     AKELRDQATSLATEILQIALETDCPFNFDQRIYKILEKYSVPFDAEKGSFEKRWTTYW
                     KKRPSLSLESSRDPKKVRTEIRKLGEKLLNESDKEGLRKKAIEIAEKYLELSEYGLAY
                     ATAAILKDENKSDDAGIRAARLQHELDFARLPLTQIAPGTLTATQRWDLARYLAAIAK
                     SCSNLGLNEGDCEWFSQAPKVLLRWN"
BASE COUNT          406 a          424 c          447 g          565 t
ORIGIN      
        1 attggtttac ccgacaattt caactgcgat ggaccgccga gatccatgac tgtcaccgga
       61 tcagtggctt ttttccctgc ttttataccc aagtgaagtc cgccaacgcc ctttccattt
      121 aagtgaatgt gcgcggaagg actttgtccg atgagcgtaa gtccctggtg caaaacatgt
      181 gattgggcga cttcttcgcc tgcgtctaaa atctcgaggc gagtagtcat taccgcgggg
      241 gctgtttttt cggcggattt gtttttttca gtaacttgca tacagcttta agcctcaaag
      301 tttaacgcac atactcgtaa gaacctaaaa tgcgattttt ctggccatgt tttacgcgat
      361 acagatgttt aaattcgagg tcagctccgt gtagcaaata ggcggcgttt ggtttcttaa
      421 cctttccttc aatgctggct tcttcgaaat caaccactgt gcgagagcgc actttggtaa
      481 caactcgttt ttcggtggtt agccccttac tgtcggtggt gcgggtaact tgagtgtcga
      541 ccacttcggt gtcgccgcgg tcctggccaa attctgctgc gtgggtggcg cgtcccaaga
      601 gtagtgcaca cattaaaatg gacccggcat acacagcagt tttaagacga ctcatggccg
      661 cccccttcta ggtaaagtaa ctattacagt gttattttaa attaaccgtt caaagtagtc
      721 aattataaca aaaagtcaat aaatacagca agttacagtt aattccaacg taacaggacc
      781 ttaggcgcct gactaaacca ttcgcagtca ccttcattta gcccaagatt cgagcacgat
      841 ttcgcgatgg ccgcgaggta acgggctagg tcccagcgct gggtagccgt cagggtgccg
      901 ggggcaattt gggtgagtgg tagtcgagcg aaatcgagtt cgtgttggag ccgggcggct
      961 cgaattccag catcgtcact tttgttttca tctttcaaaa tcgcggccgt ggcgtaggcc
     1021 agaccgtatt ccgaaagctc caagtatttt tctgctattt ctatagcctt ttttcgaagc
     1081 ccttctttgt ctgattcgtt gagcagtttt tctccaagtt ttcgaatctc agtgcgcact
     1141 tttttggggt cacgggaact ctctagcgat agcgagggtc gcttcttcca gtaagttgtc
     1201 caacgttttt caaacgaccc tttttcggcg tcgaacggaa cactgtattt ttcgagaatc
     1261 ttgtatattc gctgatcgaa attaaatgga cagtcggtct cgagggcgat ttgaagaatt
     1321 tcggtagcca gcgaggttgc ttgatcgcgc agttcctttg cgttgcccat gatggtctcg
     1381 acgtatgctt ttcggtccgc ttcggtggct tgatttaaaa ctgcgggaag ggctgccgtt
     1441 tctagcgttt ccgcgaactc gcccaaaagt tcggttttta caatagtcgc ccagagtctg
     1501 gcagcgccac gtaaagattt tggggtgcga tcgactgttt tttcgagcgt ggcgtttaga
     1561 attttatgtt gatcggcctt tttcttaaaa ttactttgtg ggttgttgtt gcttttcaca
     1621 aaccgaagct tgaaaaaatc cccaagactt ttttcgaacc gctggaattc ggcccaggtt
     1681 ttttctacat ctgcatcgct gttgacgacc tttgcaccgc cttgaataaa tttagtgtga
     1741 ttgagatcgc ggatggtttt taagttctgt tcagttgccg acctagtttt ggaatcgagt
     1801 ttcaatttaa gcaactcttc gtacgagttt ccaagttcaa ac
//
LOCUS       DAOMXO010000505         1840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37394_length_1840_cov_3.952381, whole genome shotgun sequence.
ACCESSION   DAOMXO010000505 DAOMXO010000000
VERSION     DAOMXO010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37394_length_1840_cov_3.952381"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLH57_09680"
     CDS             complement(<1..657)
                     /locus_tag="PLH57_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRK02921.1"
                     /translation="MKSSSGKAPSKGFDGRKITTASGIPVDRIYTQTVKADALGNPGE
                     YPFTRGVQRDMYRGRLWTMRQYAGFGSAKESNKRYKYLLAQGQTGLSVAFDLPTQLGL
                     DPDHQRAKGEVGKVGVSIACLDDMRRLFDGIDQAKVSTSMTINATAGTLLALYVAVAK
                     ERGANLRELRGTVQNDLLKEFAARGNYIYPPRPSLKLTTDVSTPPASTSLPVSNRSET
                     G"
     gene            complement(654..1151)
                     /locus_tag="PLH57_09685"
     CDS             complement(654..1151)
                     /locus_tag="PLH57_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRK02922.1"
                     /translation="MKYWVTCAGSEQLFDELESGVRTNAKVDRFGSFTRVGLDQESCY
                     FGVTVRSGSMPGERRVLIACGLGNIEFGIERGVGTRSDAQGSTGSKILKSSMPGRIVR
                     VLCKPGDRVSAGQPLLVLEAMKMENEIAASVSGLIEKVAVTADQKIETGEILLTIDAG
                     SASKT"
     gene            complement(1148..>1840)
                     /locus_tag="PLH57_09690"
     CDS             complement(1148..>1840)
                     /locus_tag="PLH57_09690"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014804.2"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRK02923.1"
                     /translation="TLEFLADDNGGFYFLEMNTRLQVEHPVTEWVTGLDLVERQFRIA
                     AGETLAKGPIVTGQNGHAIEVRVYAESPFDYLPSGGAIDGLVLPQGPFVRVDSSIYTG
                     YNVPTEYDPTLMKLSVWGSDRAAAVAQLRSALDELRILGVTHNQGLFWAICRERNFIE
                     GNYGTPYLEKNRSELLKIYGTELERLKKFFRPIFENSYDGRLATAQSNAIHSIDSNWE
                     RSARSESLRGNL"
BASE COUNT          437 a          513 c          419 g          471 t
ORIGIN      
        1 cccagtttca gagcgattgg agacaggcag agatgttgat gcaggaggag ttgagacgtc
       61 agtcgtcagt ttaagtgatg ggcgcggtgg ataaatataa tttccccgcg ctgcaaattc
      121 ctttaggaga tcgttttgaa ccgtgccacg cagttctctc aaattggcac cacgctcctt
      181 ggccaccgca acgtaaagtg ccagcaaagt tcctgctgtc gcattaatag tcatcgatgt
      241 gcttacttta gcctgatcta ttccgtcgaa tagtctacgc atgtcgtcta agcacgcgat
      301 cgagacgcca acttttccta cctcgccctt ggcgcgttgg tggtccgggt ctaatcccaa
      361 ctgggtgggc aaatcaaaag ccacgctaag tccggtttgc ccctgtgcga gcagatactt
      421 gtaacgttta tttgattcct tcgccgatcc aaagccggcg tattggcgca tggtccataa
      481 tcgacctcga tacatgtctc gctgcacgcc gcgagtgaac gggtactcgc ccggatttcc
      541 aagcgcgtcc gctttcacgg tttgggtata aattctgtct accgggatac ccgaagccgt
      601 agttattttt cgaccgtcga accctttcga tggtgctttt cctgaactcg atttcatgtt
      661 tttgaagcac tcccggcgtc aattgtaaga agaatttctc cggtttctat tttttggtct
      721 gcggtcaccg ccaccttttc gatcaaccca gaaaccgagg ctgcaatttc gttttccatt
      781 ttcatagcct cgagtaccaa tagtggctgt cccgcagaaa cacgatcgcc gggtttgcac
      841 aaaacacgga ctatgcgtcc gggcatcgaa ctctttaaga tttttgaacc cgtgctccct
      901 tgtgcgtccg atctagttcc gacgccacgt tcaatgccaa attcaatatt tcccaagcca
      961 caagcaatca aaactcggcg ctcacctggc atactcccac tgcgaaccgt aacgccaaaa
     1021 tagcagctct cttggtcaag cccaacgcgg gtaaaagaac caaaacgatc caccttggca
     1081 ttggtgcgaa cgcctgactc tagctcatcg aacaattgct cgcttccggc acaagtaacc
     1141 caatatttca caagttacct ctcaacgatt cggagcgagc tgaacgttcc cagtttgaat
     1201 cgatcgaatg aattgcattg gactgggccg tggcgaggcg cccatcatag gagttttcaa
     1261 aaatcggtct aaaaaatttc tttaaccgct caagttcggt gccgtaaatt ttcaaaagtt
     1321 ccgatcgatt tttttccaag taaggcgttc cgtaatttcc ttcgatgaaa tttctttcgc
     1381 gacaaatggc ccaaaaaagc ccttggttgt gagtaacgcc caggattctg agttcatcga
     1441 gtgccgatcg tagctgcgct actgcggcgg cccggtcact gccccataca gaaagtttca
     1501 tgagtgtggg atcgtactcc gtcggtacgt tgtaaccagt gtaaattgac gaatccactc
     1561 gaacaaatgg cccctggggc aataccagcc catcgatcgc gccacctgac ggcaaatagt
     1621 cgaatggtga ctcggcgtag acccgcactt caatcgcgtg cccattttgc cccgtcacga
     1681 ttggcccctt cgccaacgtc tcgcccgcag ctattcgaaa ctgccgctca accaaatcaa
     1741 ggccagtcac ccactcggtc acgggatgct caacctgtaa ccgggtgttc atttctaaga
     1801 aatagaatcc gccattgtca tctgccaaga actctaaagt
//
LOCUS       DAOMXO010000506         1833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37556_length_1833_cov_5.229471, whole genome shotgun sequence.
ACCESSION   DAOMXO010000506 DAOMXO010000000
VERSION     DAOMXO010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37556_length_1833_cov_5.229471"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1027)
                     /locus_tag="PLH57_09695"
     CDS             complement(68..1027)
                     /locus_tag="PLH57_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02924.1"
                     /translation="MKTLVTKLALVSALILPGVALADKVSYDKQLSDAKASLKYADTF
                     VGYNVMLTEGQLEVVDETLDRIREKRKELLNSIEKQEAARKKSVEATLRAAMKGQEEK
                     QILAAVKGAQDVIAADYKAGKEALLKLNKSLDKWKRQNLRAADHYEIPNSGGKIQQRF
                     FTIQELQDEVEGRMLSAQDLKFAGAKDSEKPNMPEIDETLEENSYRYNYKTNRDLVGN
                     VGFQLTTSMDRSSLMASAAINASNSVALLESDKTKAIDEIKAAVEKQTGMALKKATSN
                     KVEAEVETEEKAPARKKSRRSSDDDSAPASIPEDSGSTGTGLD"
     gene            complement(1265..>1833)
                     /locus_tag="PLH57_09700"
     CDS             complement(1265..>1833)
                     /locus_tag="PLH57_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02925.1"
                     /translation="NMKNFGAFCLVVSSLFVALPLTSFAVEIAELEQEFYPITDTEIT
                     RSLGAYASILKTTYAAFRQEKATLSILETTLTKDPNTAALIHSATDSLTNLHESLGET
                     YALFKAANDEIAVNRNPNGYWNLEKKFLSAFGRTMALLAVVQTKTSGLGPVVSPPPAK
                     VVVPKKKSKKRKSSASDWEQEKVVIDGF"
BASE COUNT          405 a          431 c          447 g          550 t
ORIGIN      
        1 aaaagcaaaa agggtcgggg tttaactccc gacccttttc gagtcttttt agactgtagt
       61 ttcgattcta gtcgaggcca gttccagtcg aaccactgtc ttctggaatg ctggctggtg
      121 cgctgtcgtc gtccgaggac cggcggcttt tcttgcgagc cggtgctttt tcttcggttt
      181 ctacttcagc ttcaaccttg ttcgaagtgg ctttcttaag agccattccg gtttgtttct
      241 caactgcagc ttttatttcg tcgatggctt tagttttatc gctttcaagc agagcaacgc
      301 tgttgctcgc attgatggca gcgcttgcca tgaggcttga gcgatccatc gaagtagtta
      361 attggaaacc aacattgcct acaagatcgc ggttggtctt gtagttatac cggtagctat
      421 tttcttccaa agtttcatcg atttcaggca tgttgggttt ctcggaatct tttgctccag
      481 cgaactttag atcttgagcc gagagcatgc gcccttcgac ttcgtcttgt agttcctgaa
      541 ttgtgaaaaa gcgttgctgt atttttccac cgctgtttgg aatttcatag tggtcggcag
      601 ctcgaagatt ttgacgcttc cacttgtcta acgatttgtt gagcttcaac aaggcttctt
      661 ttccggcttt gtagtctgcc gcaattacgt cttgagcgcc tttgacggct gcaagaattt
      721 gtttttcttc ttggcccttc atagcggctc gcaaagtggc ttcaacactt ttctttcgag
      781 ctgcttcttg tttttcgata gagttcaaaa gctcttttcg cttctcgcga atacgatcga
      841 gagtttcgtc aaccacttca agttggcctt cggtgagcat cacgttgtag cccacgaagg
      901 tatcagcgta tttcaacgaa gctttggcat cgctgagttg tttatcgtac gaaactttat
      961 cggccagcgc tacgcctggc aggatcaatg cgcttaccag cgcgagtttt gttacgagcg
     1021 ttttcatagt ccttctcctt taaccaatca gtaatcaaat aaaccaatgt cttgaccata
     1081 gacaaagcaa gtccagtgcc atcccaaacc aacagggggc gggccttgga ccccgtcgaa
     1141 accaccgaat ctggtaggca ggtgtctaaa acttagacgt cgtaaaaaaa gacaggttaa
     1201 cgcgctttgt tataggcgtg ttaacctgca aattgaagtg aaatcccttt tgcgtaaaac
     1261 cgggctaaaa accgtcgatc actacttttt cttgttccca gtcgctggcg gaagatttgc
     1321 gttttttgga ctttttcttg ggcactacaa ctttggcagg cgggggcgag accacaggcc
     1381 ccagaccgct ggtcttcgtc tgtacaacgg ctagtagcgc catggttcgg ccaaaggccg
     1441 aaagaaattt cttttcgaga ttccagtacc cgttcgggtt tcggttgact gcaatttcat
     1501 cgttcgcagc tttgaatagt gcgtaagtct cgccgagtga ctcgtggaga ttggtcaacg
     1561 aatcggtagc gctatgaatc aaggcagcgg tgttcgggtc cttggtgagc gtagtctcaa
     1621 gtatagagag ggtcgctttt tcttggcgaa acgccgcata ggtggttttc aaaatcgaag
     1681 cataggcacc gaggctccgc gtgatttcgg tgtccgtgat tgggtagaat tcctgttcga
     1741 gctcggcaat ttcaactgca aaactcgtga gcggaagtgc aacgaataaa gaactaacaa
     1801 caaggcagaa cgccccaaaa tttttcatat ttc
//
LOCUS       DAOMXO010000507         1833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37559_length_1833_cov_4.777840, whole genome shotgun sequence.
ACCESSION   DAOMXO010000507 DAOMXO010000000
VERSION     DAOMXO010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37559_length_1833_cov_4.777840"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLH57_09705"
     CDS             complement(<1..853)
                     /locus_tag="PLH57_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02926.1"
                     /translation="MNKRRIALSVDHQLCSGDNQLNSPVILMTGTARAGKTTLGRIMG
                     SCKNVEFFDEPWMLMTLIISAGLSLIDRNLAIGMFKTYFYELINDRILMRAVSFRPTD
                     LSYIGHQKAPEEIKNRMLKLHSRNDVRKYILEKKTVIVLNLTDTTPYLSLLKEIFPDS
                     PIIHVFSPGYCVAKETTLKGWYTDSELKRPTHGQLYRPVSISKSNRNNKRYFHFWVRP
                     GDEQEFLKRSEFARGLYYWRRIIEMSKLPKVSVKNDSKLISFNFYDALNDPKRIRSLL
                     KKMNFKPT"
     gene            complement(889..>1833)
                     /locus_tag="PLH57_09710"
     CDS             complement(889..>1833)
                     /locus_tag="PLH57_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRK02927.1"
                     /translation="KLSFQASYASFDVPKSENLAALVDTLRNSSFYLGGSVTMPYKIG
                     IMKYLDEVDPTARAIGAVNTIVRTKNGKLVGYNTDGIGGVESIKKGGVELANARTLLI
                     GAGGAARSLAFCIAKASRSGKLWITNRTEEKALTLVKDLKREFNNVDFIPFEEIAEIL
                     PQLQLLVNASTVGQSGLQKNAKGDFVNLGPFSPLSRVEPPSLMVQGSVSNPEIERELL
                     SSSLDSIMKNSEESLKRCLLLNRQAIVFDAVYSPLETVFLAHSRQTGHKTINGKMMNI
                     FQAADAMFNKVFHEYLSSRGLWTETTYKKIVDQMQLVW"
BASE COUNT          511 a          407 c          357 g          558 t
ORIGIN      
        1 gagtcggttt aaaattcatc ttttttaaca atgacctgat tcgttttgga tcattaagtg
       61 catcgtaaaa attaaacgag atcaattttg aatcattttt gacactcact tttggcagtt
      121 tgctcatttc aattattcga cgccaataat aaagtccccg agcaaattcg gaacgcttaa
      181 gaaattcttg ctcatcgccc ggtcgcaccc aaaagtgaaa gtatctttta ttatttctgt
      241 tggatttaga tatcgaaacg ggccgataga gctgcccgtg ggtaggcctc tttagttccg
      301 aatcggtgta ccatcccttt agggtggttt ccttcgccac gcaataaccc ggactaaaaa
      361 catgaataat ggggctatct ggaaagatct ctttcaaaag acttaaatat ggcgtcgtgt
      421 cggtgagatt taatactatg accgtttttt tttccaaaat atactttcga acgtcattgc
      481 ggctatgcaa cttaagcatt cgatttttaa tttcttcggg tgctttttga tgcccaatgt
      541 aacttaaatc agtgggtcga aaactgacag ctcgcattag aatgcgatcg ttaattagct
      601 cataaaaata ggttttaaac attccgatcg ccagattacg atcgattaag gagagccccg
      661 ctgagataat gagcgtcatc aacatccagg gttcgtcaaa aaactccaca ttcttgcagg
      721 aacccataat tcgacccaac gtggtttttc cagccctggc tgttccggtc atgagaatta
      781 caggggaatt gagttggtta tctcccgagc acaattggtg gtctaccgaa agagcaatgc
      841 gtcttttatt catgtttttt aaaaatcact tctttcgtta accggtgatc accagacgag
      901 ctgcatctga tcgacaattt ttttgtaggt agtctcggtc cacaaccctc gactagacaa
      961 gtactcatgg aaaaccttat taaacatggc gtcagcagcc tgaaaaatat tcatcatttt
     1021 tccattaatg gtcttatgac ctgtctgcct tgagtgcgcc aaaaatacag tctctagtgg
     1081 tgaatatact gcatcgaata caattgcttg ccgatttagc aataaacacc gtttcagtga
     1141 ttcttctgaa ttcttcataa tcgaatccaa cgagctggaa agaagttcgc gttcaatctc
     1201 aggatttgaa acactgccct gcaccatcaa gctagggggt tccacacgag aaagagggga
     1261 gaaaggtccc agatttacaa agtcgccttt agcatttttt tgaaggccac tttgacccac
     1321 ggtggaggca ttcaccagca attgcaattg cggtaatatc tctgcaattt cttcgaatgg
     1381 gataaaatcc acattattaa attctcgttt taagtctttt accaaagtta atgctttttc
     1441 ttctgtccga ttggtaatcc aaagttttcc cgaacgagag gctttcgcga tacagaatgc
     1501 gagcgatcga gcagctccac cggctccaat taatagcgtt ctagcatttg cgagctctac
     1561 gcctcccttt ttaatcgact cgactccacc tatcccgtcc gtattatacc ctactaattt
     1621 tccgttttta gttcgtacaa tagtgttaac agcaccaatt gcacgcgcag taggatcgac
     1681 ttcatcaaga tatttcatta tgccaatttt gtatggcatc gtgacactcc cacccaaata
     1741 aaacgaagaa tttcgcagcg tgtctactaa tgcggctaag ttttccgatt taggcacgtc
     1801 aaacgaagcg taagaagctt ggaagcttaa ttt
//
LOCUS       DAOMXO010000508         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37584_length_1832_cov_5.590321, whole genome shotgun sequence.
ACCESSION   DAOMXO010000508 DAOMXO010000000
VERSION     DAOMXO010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37584_length_1832_cov_5.590321"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLH57_09715"
     CDS             <1..196
                     /locus_tag="PLH57_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRK02928.1"
                     /translation="DQVNSNLANFESVKRFKILPVEFTVESGELTPSLKVKRKFCCEK
                     YRAEIDSMYPQASAAESRAI"
     gene            272..475
                     /gene="rpoZ"
                     /locus_tag="PLH57_09720"
     CDS             272..475
                     /gene="rpoZ"
                     /locus_tag="PLH57_09720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRK02929.1"
                     /translation="MARVTIEDCLDHVDNMYELVHVATQRARQLYNGSGRTVRCKNKE
                     VVAALREIAAGHVDVAEDRSDIN"
     gene            complement(487..>1832)
                     /gene="lon"
                     /locus_tag="PLH57_09725"
     CDS             complement(487..>1832)
                     /gene="lon"
                     /locus_tag="PLH57_09725"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRK02930.1"
                     /translation="GSIICLVGPPGVGKTSLGKSIARSMGREFFRFSLGGMRDEAEVK
                     GHRRTYIGAMPGKILNAMKRSGSVNPVLVLDEIDKLGSSFQGDPASALLEVLDPEQNQ
                     SFLDHYLDVPFDLSKVLFIVTANTTATIPGPLLDRMEVIELNGYTLEEKQKIALAHVV
                     PKELSAHGLTAKQLELSHDALEKIVTGYAKEPGMRSLQQKIATICRKVAAKVVENPEI
                     GKIKISSRNLYDYLGPERFENEVLRRVQNPGVVVGLAWTPLGGDILFIEAGDIPRHIK
                     TEQGLSIAASGSPAREVSYGHLKITGKLGDVMNESAAIAYSYVKKKTRRNKAAQAFFE
                     ANDIHLHVPSGAIPKDGPSAGVTMATALLSLVTGKKVRRDVAMTGELSLVGKVLPVGG
                     IREKVLAAKRAGIKTVVMPKQNKKDLRELPKQNIKGLKFVFAETVEDVFKSAFEN"
BASE COUNT          451 a          432 c          432 g          517 t
ORIGIN      
        1 ggaccaagtg aattcgaatt tggcgaactt tgaatcggtg aagcggttta agattctgcc
       61 cgttgagttt acggtcgaaa gcggtgagtt aacgccttcg ctcaaggtta aacgcaaatt
      121 ttgttgcgaa aagtaccggg cagagatcga ttcaatgtac cctcaggctt ccgcggcaga
      181 atcacgggct atttagagta aaatagtctg gtatgcttga cccggacgcc aagtcgcggt
      241 attgaattga aaatttttca gagaggtatt tatggcacgc gttactatcg aagattgttt
      301 agatcatgtg gataacatgt atgaattggt ccacgttgct acgcaacgcg cgcgccaact
      361 ctacaacggc tctggtcgca cggttcgctg taaaaacaaa gaagtagtag ctgcattgag
      421 agaaatcgca gccggccatg ttgacgtggc tgaagatcgc agcgatatca attaatttcg
      481 cttctattaa ttttcgaagg cagatttaaa aacgtcttcg accgtttcgg caaaaacaaa
      541 cttaagacct ttgatgtttt gcttcggcaa ttcacgaagg tcttttttgt tttgtttcgg
      601 catcaccacg gtcttgatac cggctcgctt agcagcaagt actttttctc gaatgcctcc
      661 aaccggaaga acttttccaa ctagcgatag ttcacccgtc atggccacat ctcttctaac
      721 ttttttaccc gttaccaaag acaacagcgc agtggccatt gtaactccgg ctgacggacc
      781 atctttagga atggctcccg agggcacatg caggtggata tcgtttgcct caaaaaaagc
      841 ctgagctgct ttattacgcc gagttttttt cttcacataa ctataggcaa tggctgcaga
      901 ctcgttcatt acgtcaccga gttttccggt gattttgaga tggccatacg acacttcgcg
      961 cgccggagag ccactggccg ctatcgagag cccctgttcg gtttttatat gccgaggaat
     1021 gtcgcccgct tctataaaaa gtatgtcgcc tccgagtgga gtccaagcta ggcctactac
     1081 aacgcccgga ttctgaactc gtcgcagaac ttcattttcg aaacgttcgg gccccagata
     1141 gtcgtacagg tttcgagaac tgatttttat ttttccgatt tctggatttt ctactacctt
     1201 agccgcgacc ttacggcaga tggtggcaat tttttgttgc aacgaccgca tgccaggttc
     1261 tttcgcataa ccagtgacta ttttttccag tgcgtcgtgg gaaagctcta attgttttgc
     1321 cgttaatccg tgagcggaaa gttctttcgg aactacgtgg gccaacgcaa ttttttgttt
     1381 ttcttcgagc gtgtaaccat tgagttcgat gacttccata cggtccaaga gtgggccggg
     1441 gatcgtggcc gtagtgttag ccgtaacaat aaaaagcacc tttgaaagat cgaacggtac
     1501 gtctaaataa tggtcgagaa aactttgatt ttgttcgggg tctaatactt cgagtaatgc
     1561 tgaagcaggg tcaccctgaa aactgctgcc taatttgtcg atttcatcga ggaccaacac
     1621 cggattcacg gatccagagc gcttcatcgc gtttaggatt tttcctggca tagctccaat
     1681 gtaagtacga cgatgccctt tgacttccgc ttcgtcgcgc attccaccaa gtgaaaatcg
     1741 aaaaaattct cgtcccattg aacgggcgat cgattttccg agtgaagttt ttccgacccc
     1801 tggaggcccg acaaggcaga taatcgaccc ct
//
LOCUS       DAOMXO010000509         1831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37620_length_1831_cov_5.530968, whole genome shotgun sequence.
ACCESSION   DAOMXO010000509 DAOMXO010000000
VERSION     DAOMXO010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37620_length_1831_cov_5.530968"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLH57_09730"
     CDS             complement(<1..390)
                     /locus_tag="PLH57_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02931.1"
                     /translation="MNSMKRVLNLECLFATVVFAVAVPIADSAELAKAISQSENEPSA
                     LRAAISTMTEESLEQQYYHFARIAHVQELLGRRKMNQTKAHLKTADSKRLEKFVHDHA
                     LKRLRGKWKPQAAKVARTILEESRKHQF"
     gene            603..1670
                     /locus_tag="PLH57_09735"
     CDS             603..1670
                     /locus_tag="PLH57_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRK02932.1"
                     /translation="MNLSLSIDNSLLLTLAAILVSFGLVFLNGFFVAAEFAIVKVRRT
                     RLEELAGQGVAQARVSLRCVDQLDESLSATQLGITLVSLALGWLGEHAFASLLAHLAP
                     SFASGGAHHVVAVVVAFGIITVLHVVLGELVPKSMAIQEAEKVTLWVARPLSLFYRFS
                     RPAIRTFTAMANFILRRLGYFGLEDEPLTENELKLVMKESHEDGVISESEAQIINRAF
                     EFSDRRVRELMVHGDKVDFISLAKPLDQNLAVVRRHMHTRFPLCREGFDTVIGVVHMK
                     DAWPLLLTNFSNETFEKMARPAIFVDPALRQDQLLKIFQNRRGHLAIVADPRTKQNVG
                     IITMEDVLECLVGEIRDEHGN"
BASE COUNT          413 a          433 c          503 g          482 t
ORIGIN      
        1 aaactggtgt ttgcggctct cttctaggat tgttcgagcg accttcgctg cttggggttt
       61 ccatttaccc cgcaggcgtt taagagcgtg atcgtgcacg aatttttcaa gccgtttgga
      121 gtcggccgtc tttaagtggg ccttcgtctg gttcattttg cggcggccta agagctcttg
      181 tacgtgggcg atacgggcaa agtggtaata ctgctgctct aacgattctt ctgtcatcgt
      241 gctgattgcg gcgcgcaaag cagagggttc attctcggac tgagaaatgg cctttgcaag
      301 ttcggcactg tcggcgatcg gtacggcgac cgcgaatacg accgtagcaa agagacactc
      361 aagatttagg actcgcttca tggagttcat aatgaaaacc cctcctgacc ctagataatg
      421 caatgggtgg gccagcgagg ggtgtgtcgg cctaagttat tgtatttaaa gtgatttatt
      481 tgaagcccca ggcccagcca aggggggcgg ccggagcgaa ctcgacaggt gtcaaccccc
      541 gttactgagt agaattaacc tgtgtcacct tagctaaata tctggtatga ttggaaaatc
      601 tcatgaatct ttccctaagt atcgacaata gcttactttt aacccttgca gcgattttgg
      661 tttccttcgg actggttttt ttgaacggct ttttcgtggc cgccgaattt gccatcgtga
      721 aggtacgccg tactcggttg gaagaactgg ccgggcaagg ggttgcgcag gcccgtgtct
      781 cactgaggtg cgtggatcaa ctcgacgaat cgctctcggc cacacagctg ggaataacgc
      841 tcgtcagcct agctttgggc tggctcggag agcacgcttt tgcgtcactt ctggcgcatc
      901 tcgcgccgtc gtttgccagt ggcggagctc accacgtggt agcagtggtc gtggcgtttg
      961 gcattatcac cgttttgcac gtggtattgg gggaactagt tcccaaaagc atggccattc
     1021 aggaggcgga aaaagtcact ctttgggtgg cgcgtccgct gagcctattc taccgtttct
     1081 cacggcccgc aattcgaact tttaccgcaa tggccaattt tattttgcga cgcctcgggt
     1141 acttcggact ggaagacgag ccacttaccg aaaatgagtt aaaactcgtg atgaaagaat
     1201 cgcacgagga cggagtgatt agtgaaagtg aagcccagat tattaatcgg gcgtttgaat
     1261 tctctgaccg tagggttcga gaacttatgg ttcacggcga caaagtagac ttcatctctt
     1321 tggccaagcc gctcgatcaa aacttggcag ttgttcgtag gcacatgcac acacggtttc
     1381 cgctgtgtag ggaaggattt gataccgtga ttggagtggt ccacatgaaa gatgcttggc
     1441 cactgctcct aactaatttt tcgaacgaaa ctttcgaaaa aatggcccga cctgcaattt
     1501 tcgtagaccc ggcgcttcgc caagatcagt tgctaaaaat tttccagaat cgtcgaggtc
     1561 acttagcaat agtggctgat cctcgcacca agcaaaacgt tggtatcatc acaatggaag
     1621 acgtgctcga atgtttggtc ggcgaaatta gagacgaaca tggcaactga tttgattcta
     1681 ggtccaactc cgctgggtct cgtacaccct actcaaatgc gggaagaccg ttttggaaga
     1741 ttgtgcgtcg gcggtgcaga actaccacgg gtcacggtcg tggggcggct ggaaacagag
     1801 acaggttttt ttttctgcga cttcagtatt g
//
LOCUS       DAOMXO010000510         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37667_length_1829_cov_7.022548, whole genome shotgun sequence.
ACCESSION   DAOMXO010000510 DAOMXO010000000
VERSION     DAOMXO010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37667_length_1829_cov_7.022548"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1066
                     /gene="glpX"
                     /locus_tag="PLH57_09740"
     CDS             107..1066
                     /gene="glpX"
                     /locus_tag="PLH57_09740"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRK02933.1"
                     /translation="MHRNFAMEFVRVTEMAALAAARHMGRGDEKAADQAAVDAMRRML
                     NSVDFNGTVVIGEGERDEAPMLYIGEKVGTGSGPGLDLALDPLEGTTICARGGNNSIA
                     VIAIAEEGNFLHAPDTYMEKIAVGSDARGAIDLDRSPTENLREIAKAKKKELQDLTVI
                     ILDRPRHADLISEVRRAGCRIWLIGDGDVSAAIATCKPESGVDVLMGTGGAPEGVIAA
                     AALRCVGGDFQGRLKPRNNDEIERARRMGIQDIHRVYKINELAKGNVMFCATGVTQGA
                     FLDGVRFLGGGAITHSVVMRSESGTSRYIEAEHHFDRKPRFEY"
     gene            1079..1504
                     /locus_tag="PLH57_09745"
     CDS             1079..1504
                     /locus_tag="PLH57_09745"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRK02934.1"
                     /translation="MAEASKTMEMNVSAAALWKTITNYEGYSQFVDGVKSVQVLSRAG
                     GNARVQYNIELLGKTITYTLDHVEVPEKEMTWQLVESNVMKANDGGWTIKDLGSGRVS
                     VTYRLALEFKIFVPGMILNGLVKNMLPRMMESFEKRSHG"
     gene            1497..>1829
                     /locus_tag="PLH57_09750"
     CDS             1497..>1829
                     /locus_tag="PLH57_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02935.1"
                     /translation="MAEEKRSGLSDAMKENLKETMKKVVMTGVGTIFLTEETIRNVLG
                     EIKLPKELWSGIIDNASKTKQEFMGAFAKEVAQVVSQIDIAGEAKKFFENNRMKINIE
                     LSFDPKDID"
BASE COUNT          509 a          405 c          509 g          406 t
ORIGIN      
        1 aaaaaaccac ctcagtgaca atggcagacc tgtttccgaa ccttgcactg tattggcagt
       61 tgcaaaattc gctctcctgc gtaaaagttc ggccacggag atttgtatgc acagaaattt
      121 tgcgatggaa ttcgttcgag taaccgaaat ggcagcatta gccgctgcgc gtcacatggg
      181 ccggggcgac gaaaaagcgg cggaccaagc agcggtagat gctatgcgcc gaatgctcaa
      241 ttcggtcgac ttcaacggta cggttgtcat tggtgaaggt gaacgagacg aagccccaat
      301 gctttatatt ggagaaaaag ttggaacagg ttccggtccg gggttggatc tggcgttgga
      361 tccacttgaa ggcactacga tctgcgctcg gggggggaac aatagcattg ccgtgatcgc
      421 cattgctgaa gaaggcaact tcttgcacgc ccctgacacc tacatggaaa aaatcgcagt
      481 cgggtctgac gctcgaggtg cgattgatct ggatcgttcg cccactgaaa accttcgcga
      541 aattgcaaaa gccaagaaga aagagcttca ggatttgact gttattattc tcgatcgtcc
      601 gcgtcatgcc gatttaattt cggaggttcg aagagcaggg tgccgcattt ggctgatcgg
      661 cgacggtgac gtatcagcgg ccattgcgac ctgtaaaccc gagtcgggcg tagatgtctt
      721 aatgggcacc ggaggagcac ccgaaggcgt tattgccgct gccgcactgc gctgtgtggg
      781 aggggacttt caggggcgtt tgaagcctcg gaacaacgat gaaatcgaac gcgcacgccg
      841 catgggcatt caagacattc atcgagttta caagatcaat gaacttgcca aggggaatgt
      901 catgttctgc gccacgggcg tgacccaggg cgcgtttctc gacggagtgc gatttttggg
      961 aggaggggcg attactcatt cagtggtcat gcgctctgag agcggaacta gccgctatat
     1021 tgaagccgaa caccacttcg atcgcaaacc tcgtttcgaa tactaaggga atcaagtcat
     1081 ggcagaagcc tcaaaaacaa tggagatgaa cgtctccgcc gccgcgcttt ggaaaaccat
     1141 taccaattac gaaggttatt cgcagttcgt cgatggtgta aaatcagttc aagttttatc
     1201 tcgggctggc ggaaatgcgc gagtgcagta caatatagaa ctactaggaa agacgatcac
     1261 ctatacgctc gatcacgtcg aagtgcccga aaaagagatg acctggcagc tcgtagaaag
     1321 caacgtgatg aaggccaacg atggtggttg gacgatcaaa gacctggggt ctgggcgcgt
     1381 cagtgtgact tatcggttag cgcttgaatt caagattttt gtgcccggta tgatcttaaa
     1441 cggacttgta aaaaacatgc tcccgcggat gatggagagc ttcgaaaaga ggtctcatgg
     1501 ctgaggaaaa acgcagcggt ctttcggacg caatgaaaga aaatctgaaa gagacgatga
     1561 aaaaagtcgt catgacgggc gttggaacga tattcctaac tgaagaaacg attcgcaacg
     1621 tactgggtga aatcaaattg cctaaagagt tatggagcgg gatcatcgat aacgcctcca
     1681 aaaccaaaca agaatttatg ggcgcttttg ccaaagaagt cgcgcaagtt gtgtcgcaaa
     1741 tagacattgc gggcgaagcc aaaaagtttt ttgaaaacaa tcgaatgaaa atcaatatcg
     1801 aactgagttt cgatccaaaa gacattgat
//
LOCUS       DAOMXO010000511         1828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37690_length_1828_cov_8.514946, whole genome shotgun sequence.
ACCESSION   DAOMXO010000511 DAOMXO010000000
VERSION     DAOMXO010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37690_length_1828_cov_8.514946"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1828
                     /locus_tag="PLH57_09755"
     CDS             <1..>1828
                     /locus_tag="PLH57_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02936.1"
                     /translation="ARNIRFATLSTGDVAAVFLVHDPDDLGLTTSDDRVVFAAVYSAS
                     SNSWGAATQLGTADATTDVAEDYDVGNWNTPFCAPAIAAGPDGSAMVLWCETVGTEAR
                     VFYSQYTLGAWSAAAQLITAASNVAFPGYSGYYHTFNGVVNTTTIQDGDTFTVSFDNT
                     TEGNIGNANCIADYTMTAASTEPATENEFKIYKKADGTVLVCDTMREALNRVLFADAD
                     DPLDTLTLHQCGVSTWINPGCVDTDTATWDFAVRYNQSLAAKYRTIGEADDNSVLAVA
                     DTFGNMPIDAGTLQTNVAPYSRAAVVDVAADGYGNYLAVRSVVAPYFDDNIGSTVGNG
                     RQLVGHVYLASSGWVYRSGGTDPEAYLISRTPACYTGRTATSGATYVACSVRQPKALM
                     SENGHGLVLYYQNQRDTVSSTVATSSDSFPNRLWYSTYSVATGFTGASATVDSDVLCD
                     SNSIRNDRSVCEGGSSGVACQLEREPEELPLDELWVDSSLPPIAADMNASGSAVIAYH
                     KDYYLSDGTCSALIGVHVALYDPVNGLGSVKQLDDGTGNTMNAAVTITESGKAAVVWE
                     QIVSGVRYVYLRSYENGTWSVQEHINSGLTATSDAASPAVKML"
BASE COUNT          429 a          474 c          505 g          420 t
ORIGIN      
        1 gagcccgtaa cattcgattc gcaacactct ccactggcga tgtagccgcg gtgtttttgg
       61 tgcacgaccc agacgaccta ggactgacaa cctctgatga tcgcgtcgta tttgctgcag
      121 tttatagtgc ttcgagtaac tcttggggag cggccaccca actcggcact gcagacgcga
      181 cgacagatgt tgcagaagac tacgacgtag gaaactggaa tacaccgttt tgtgcgcccg
      241 ctatcgcggc cggccccgac ggatcggcca tggttctgtg gtgtgaaaca gttggcacgg
      301 aagcgcgagt gttttactcg caatacacgc tgggtgcttg gtcagcggca gcgcaattaa
      361 taactgccgc ttcgaacgtc gcgtttcctg ggtattccgg ctactatcac acgttcaacg
      421 gcgtggtgaa caccacgacc attcaagacg gtgacacgtt taccgttagt tttgacaaca
      481 ctaccgaagg caacattggc aatgccaatt gcattgcaga ttacacgatg acggcagcct
      541 ctacagaacc agcgactgaa aacgaattta aaatttacaa aaaagccgat ggaaccgttt
      601 tggtgtgcga caccatgcgc gaagcactca atcgtgtttt gttcgccgat gccgatgacc
      661 ctttagacac tttaaccctg caccagtgtg gcgtgagcac ttggatcaac ccaggttgcg
      721 ttgacaccga cactgcaact tgggactttg cggtgcgcta caatcagtcg ctggcggcga
      781 aatatcgaac cattggtgag gcagacgata actctgttct ggcggtggct gatactttcg
      841 ggaacatgcc gatagatgct ggcacacttc aaacaaacgt cgcgccctac agtcgcgctg
      901 cggtggtcga tgtagcggcc gatgggtacg ggaattactt ggcagttcgc tcagtggtgg
      961 cgccatactt cgacgacaac attggttcga cggtcggtaa cggtcgccaa ctcgtgggtc
     1021 acgtttactt agcgtcgagt ggttgggtct atcgttcagg tggcaccgac cccgaagcct
     1081 atctgatttc gcgaacgccg gcgtgttaca ccgggcgcac ggctacaagt ggcgccacat
     1141 acgttgcatg ttcggtgcgc cagcccaaag ctcttatgtc tgaaaacggt cacggactgg
     1201 tgttgtacta tcaaaatcag cgggacacgg tctcaagtac ggtcgccact tcgagtgatt
     1261 cttttccgaa tcgcctttgg tattccactt attccgttgc aacaggattt actggtgctt
     1321 cggcaacggt ggactcagac gtactttgtg atagcaactc cattcgcaac gatcgttcag
     1381 tgtgtgaggg aggatcgtcc ggggtagcgt gccagcttga acgcgaacca gaagaactgc
     1441 ctctagacga attgtgggta gattccagtc tgcctccaat agcagcagac atgaacgcta
     1501 gcggaagcgc agtcattgcg taccataaag attactattt gagcgacggt acttgtagtg
     1561 cattgatcgg cgtgcatgtg gcgctttatg atccagtgaa tgggctcggt agcgtaaaac
     1621 aattagacga cggcacgggt aacaccatga acgcagccgt gacgatcacg gagagcggca
     1681 aggccgcagt ggtttgggaa caaattgtat cgggagtccg ctacgtttac ctgcgatcct
     1741 acgaaaacgg tacgtggtcg gttcaggaac acatcaactc aggactcact gcaacctccg
     1801 acgccgcaag cccagcagtt aagatgct
//
LOCUS       DAOMXO010000512         1828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37706_length_1828_cov_3.429216, whole genome shotgun sequence.
ACCESSION   DAOMXO010000512 DAOMXO010000000
VERSION     DAOMXO010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37706_length_1828_cov_3.429216"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..773
                     /locus_tag="PLH57_09760"
     CDS             438..773
                     /locus_tag="PLH57_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HRK02937.1"
                     /translation="MLLSGIFGNETAEKVLLFLENYEFGYPRGIAKTFDLSISQVQNQ
                     LERFEREGIVASRLVGKTRMYEWNPRYLFLPELRAMLEKALKRLPESLQEKYFRARTR
                     PRRKGKPLP"
     gene            770..1297
                     /locus_tag="PLH57_09765"
     CDS             770..1297
                     /locus_tag="PLH57_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02938.1"
                     /translation="MINTKTTIEELGAIVCQALKDAGIEAFLSGGAVVSIYTKNKYES
                     YDLDFITFGDRKKIKKIMEALGFVQDKSRHFIHPKSVYFVEFPGSATMIGGERISEFH
                     ERKVKTNTLKLLTPTDCIKDRLAAYIHWNDKQGLEQAVMVAQNQEFKQTKVKQFCKSE
                     GNVGAYEDFLERLRK"
     gene            1342..>1828
                     /locus_tag="PLH57_09770"
     CDS             1342..>1828
                     /locus_tag="PLH57_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRK02939.1"
                     /translation="MCGRAYETFTEDELEMRYQWARTKRNPLGLQPNYNLCPTQNAPV
                     VVRSADENTVKLMRWGLVPNWAKTIKDASKYSLINARGEEITEKRSYKNAFANQRCIV
                     PLSGFFEWRRETEKGPKVPFAIYLKDEPIMSIAGIWERWHSEDSKEEVYSFSIVTTEA
                     NS"
BASE COUNT          554 a          398 c          415 g          461 t
ORIGIN      
        1 ctcagtcggt agagcaggtg actgttaatc accatgtcgg gggttcgagt ccctcctcct
       61 gagccattta aaaaaaataa aaccctgcca gcgcaagctg gtggggtttt tattttttat
      121 ggcgttatga aagaggactc gaacgcggag tgtgcggcgg agcgggtaac gccgccgagt
      181 aggcggcgtt tcgcgcccgc aaaacgcgca cgatgcgcgt tttagcgaac gaaggggagc
      241 ctacgcaacg aggagccgga ggcgacgagt gtagtaggtc gagtccctcc tcctgagtca
      301 atttaaaagt ccagttaatt caagggttta actaggattt ctttttaggc tagttgcctg
      361 agtcaattta atcgaatctt ttagcaaact cgatatattg acctaaaatt taggttatat
      421 taccctatat ttaggttatg ttactttctg gcatattcgg aaatgaaact gcagaaaaag
      481 ttctcctgtt cttggagaac tacgaattcg gctatccacg aggaatcgca aaaactttcg
      541 atctttctat cagccaagtt caaaaccaac ttgaacgctt tgagcgcgag ggaattgtag
      601 caagccggct tgttggaaaa acaagaatgt atgagtggaa tccaaggtac ttattcttac
      661 ccgaactacg cgcaatgctt gaaaaagcgc taaaacgcct tccagagtcc ctacaagaaa
      721 aatactttcg agcacgaaca cgtccgagaa gaaaaggaaa gccgcttcct tgataaacac
      781 aaagacaacc atcgaagaac taggagccat tgtttgccaa gcactgaaag atgccggtat
      841 cgaagccttt ctttccggag gtgctgttgt ctcgatttat actaaaaata aatacgaaag
      901 ttatgatctt gatttcatca cttttgggga tcgcaagaaa ataaaaaaaa taatggaggc
      961 tttaggattc gtacaagaca agtcacgaca cttcattcat cccaaatcgg tatactttgt
     1021 ggagtttcct ggatcggcaa caatgattgg aggcgaacgc atctctgaat tccatgaacg
     1081 aaaagttaaa acaaatacct tgaagctact tacacccacc gactgcatta aagatcggct
     1141 agctgcctat attcactgga acgacaaaca gggtttggaa caagccgtaa tggtcgccca
     1201 aaatcaagaa tttaagcaaa ccaaagttaa gcagttttgc aaaagcgaag gcaatgtcgg
     1261 cgcctatgaa gattttctcg agcgacttcg aaagtaggtt ggcttcttca aaccggctag
     1321 atactctctt cgcacattaa tatgtgtggt cgagcctacg aaacatttac cgaagatgaa
     1381 ctcgaaatgc gttaccaatg ggctcgcacc aaacgcaacc cgctgggtct tcaacctaac
     1441 tacaatttgt gccctactca aaatgctcct gtcgtagttc gcagcgctga tgaaaatact
     1501 gtaaaactaa tgcgctgggg attagtaccg aattgggcca aaactattaa agacgcctct
     1561 aagtattcac ttataaacgc ccgaggcgag gaaattactg aaaaacgcag ctacaaaaac
     1621 gctttcgcaa accagcgttg catagtgcca ttgagtggtt tttttgagtg gcggcgagaa
     1681 actgaaaagg gccccaaggt acctttcgcc atttatttaa aagacgaacc cattatgtcg
     1741 atcgctggga tttgggagcg ttggcattct gaagattcca aagaagaggt ttattcgttt
     1801 tcaatcgtta ccaccgaagc caattcgt
//
LOCUS       DAOMXO010000513         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37730_length_1827_cov_4.636569, whole genome shotgun sequence.
ACCESSION   DAOMXO010000513 DAOMXO010000000
VERSION     DAOMXO010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37730_length_1827_cov_4.636569"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLH57_09775"
     CDS             <1..310
                     /locus_tag="PLH57_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRK02940.1"
                     /translation="TDFRKVMVGDLVNSRTDSEVKIWAAKQTYIVMGMLLMAAAELSI
                     DACPMEGIEPAKYDEILGLAGSGFSTVSAVALGYRHAEDIFGKMKKVRKSRDELFVSL
                     "
     gene            297..1754
                     /locus_tag="PLH57_09780"
     CDS             297..1754
                     /locus_tag="PLH57_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02941.1"
                     /translation="MYRYSRVTALTGYLIETVSFVLVALSLSSPVFAASEAEMFRKKG
                     LDAYQSGNVSEAESLLRQALQRDSGAEGARDALDKIEKAKKDVKGSRNRARSAVGAGG
                     SSEENEGDSAGGTSVSTENISDTYREKGRLKPGTRSSGAAISVYDGRPIERPSEYGSS
                     SLFNRFGTGPQNFRPAAEPVAFLSRDEFIEQAYDWPDATMWNGLFRYAMTYDSNFPDV
                     VDDATVPATLQNQGAVIHQAQLQGKVRSTPLERFWYGAELNGGIKYFARSQFQPLNLT
                     PLGGGVVASWWNARSLDATFRYDAATSWLGQELFGYHSSAHGPHLSVAALAAPAIQVH
                     SGYAFRRNHFRTRPGTADLAGWRASTHSFHFEVWRVPRDSIWHPYFRTMWDWNSTQLN
                     EQRSRKKTFGVGTSLALPMIKPKGGADFSYFDFPRAVVSRIEHRWGLHAAGEMTLSQN
                     FSALLSILRDIQKSTISERYSFNRSVGSVALLYQL"
BASE COUNT          430 a          438 c          508 g          451 t
ORIGIN      
        1 caccgatttt cgaaaagtga tggtgggtga tttggtgaac agtcgaactg attccgaagt
       61 caaaatctgg gcggctaaac aaacctatat tgtgatggga atgttgttaa tggcggccgc
      121 tgaactttcg atcgacgcat gtccgatgga gggaattgaa ccggcgaaat atgacgaaat
      181 tctgggcctc gctggaagtg gtttttctac tgtttcggca gtggctctgg gttaccgaca
      241 tgctgaagac atctttggaa aaatgaaaaa ggttagaaag tctcgggacg agctgtttgt
      301 atcgttatag tcgagtgacc gcgctgacgg gctacttaat tgaaacggtg agtttcgttc
      361 ttgtggccct gagcctcagc tcgccagtgt tcgctgcgag cgaagccgaa atgtttcgga
      421 aaaaagggct cgatgcttac cagagcggca acgtaagcga agcggaatca ctgctgagac
      481 aagccctgca acgcgactcg ggcgcagagg gcgcacgcga tgcccttgat aaaatcgaaa
      541 aagctaagaa agacgttaaa ggcagtcgta accgtgcgag gtcggcagta ggcgctggcg
      601 gatcttcgga agaaaacgaa ggggattctg ccggcgggac gtcggtatct accgaaaata
      661 tctcagacac ctatcgcgaa aaaggccgtc taaaaccagg cacgagatct agcggtgccg
      721 cgatttcagt ttacgatggc cgcccaatcg aacgaccttc cgaatacggg tcatcgtcgc
      781 tctttaaccg ctttggaaca ggtcctcaga attttcgtcc ggcagccgag ccggtggcgt
      841 ttctctcgcg agacgagttc atagaacagg cctacgattg gcctgacgct accatgtgga
      901 atgggctttt tcgttacgcc atgacctacg acagcaactt tccagacgtg gtagatgacg
      961 ccacagttcc cgcaaccttg cagaatcagg gcgcggtaat ccatcaggcc cagctgcaag
     1021 gcaaagtgcg ttctactccg ctggagcgct tttggtatgg agcggaactg aacggtggga
     1081 ttaagtattt tgcgcgaagt caatttcagc cgctcaatct aacaccactg ggtgggggcg
     1141 tggtggcatc gtggtggaat gcgcgaagtt tggacgcaac ctttcgttac gacgccgcta
     1201 cgagttggct cggccaagag ttatttggtt atcatagttc cgcgcacgga ccgcatctga
     1261 gcgttgctgc attggccgct ccagcaattc aagttcactc gggctacgct tttcgccgca
     1321 accattttcg caccagacct ggaactgcag accttgcggg ttggcgggcg tcgacccatt
     1381 cgtttcattt tgaagtatgg cgcgtaccgc gcgattcgat ttggcacccc tattttcgaa
     1441 cgatgtggga ttggaattca acgcaattaa acgaacagcg cagtcgaaaa aaaacgttcg
     1501 gcgtcggaac atcgctggcg ttgcctatga tcaaacctaa aggtggagct gatttttctt
     1561 acttcgactt tccgagggct gttgttagca ggattgaaca tcgttggggg ctgcacgccg
     1621 caggtgagat gacactttca caaaattttt cagcgttgct ttcgattctc cgagacatac
     1681 aaaaatctac gattagcgaa cgctattcat tcaaccgctc ggtcggttcg gttgctttgt
     1741 tgtaccagct gtaatttctt tttcgaagta cggcgaatgg tccgccagtt gtcgtacata
     1801 ggcttctacg tcgagccatt tttcttt
//
LOCUS       DAOMXO010000514         1824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37812_length_1824_cov_4.431882, whole genome shotgun sequence.
ACCESSION   DAOMXO010000514 DAOMXO010000000
VERSION     DAOMXO010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37812_length_1824_cov_4.431882"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..>1824)
                     /locus_tag="PLH57_09785"
     CDS             complement(63..>1824)
                     /locus_tag="PLH57_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRK02942.1"
                     /translation="FGGYRSEADGLEFFLAPDGRTDPYSELVANVRAFLQPEPVKTIG
                     PLKQHPSCAFPERHRYLKEQLGLATPVVKCPDFEAWRPRFSPSGATLVFASAYLNNPA
                     SMFGHTFLRIDSKPRIDHTKKNDLLDYGVNYSAKTGDDGGITFAVLGLIGGYPGFFSL
                     LPYFEKVQEYSDVESRDLWEYPLHLEPEQIHRMIGHIWELGSTYFDYFFFDENCSYHL
                     LSLLEVANPNWHLTDEFFYWVIPSDTVRVLAKVPGALGPPTFRPSLQRRLERQYEDLN
                     PTELAQFEHARDTQFKLKGNESVAVLDVLIDQQKYDLMRNQRNETDLDKQLRRELLLA
                     RAKSLAVSDQKYSGRIDAWHSAAVSNRPDHGVHTAKLSVYGGSFRKTAYYGLQIRPVY
                     HDLFDQDAGYLAFSELTIGRVNLRIYPSEKRVRLHSAHIAEVTSLAPSDALGSRLSWS
                     ASGGLVSPRDVACRNCNALAVEGGAGESFWLSPRQSGIFYGLLKITAQYSGWFDANIR
                     FGPLVDTGALFSISDQLKTGLRARFTYFPTQKIRWSFEPTFTMGWLFANQMEFRFEQT
                     WTATKEIHGFDMNGALAWYF"
BASE COUNT          473 a          433 c          479 g          439 t
ORIGIN      
        1 gcagccgcca acgctgacgg caaccagaac ggaaatgaag tagcagatac tgattcggcg
       61 tttcaaaagt accaggcgag cgctccattc atgtcaaaac cgtgaatttc tttcgtggct
      121 gtccaagttt gctcaaagcg aaactccatt tggttggcga acaaccaacc catggtgaaa
      181 gtgggttcaa acgaccagcg aattttttgc gtcggaaaat aggtgaaccg agcccgcagc
      241 ccggttttta attggtcact aatactaaat agcgcaccgg tgtctaccag tggaccgaat
      301 cgtatattcg catcgaacca accgctgtac tgagcagtaa tttttagtaa gccgtaaaat
      361 attccacttt gacgcggcga aagccaaaag ctctcaccgg cgccaccttc cacggccaag
      421 gcattacaat tgcggcatgc cacatcgcgt ggcgacacta aaccaccgct tgcactccaa
      481 gacagacggc ttccgagcgc atctgaaggc gcaagcgaag tgacttcggc gatatgtgca
      541 gagtgtaacc tcacgcgttt ttcactggga tatattctga ggtttacgcg tcctatggtt
      601 agctcggaga atgcaagata gccggcgtct tgatcaaaca agtcgtggta caccggccgt
      661 atttgcagac catagtatgc cgtttttcga aacgagccgc cgtatactga aagtttggcg
      721 gtgtgcacgc catggtccgg acggttgcta actgcagcgg aatgccaggc atcgattcgg
      781 ccggaatatt tctgatcgga aactgcaaga gatttggcgc gggccagcag aagttcgcgg
      841 cgcaattgtt tgtcgaggtc cgtctcgttt cgttggtttc gcattagatc gtatttctgt
      901 tgatcgatca aaacatcgag aaccgccacc gattcgtttc ctttcaactt aaattgggtg
      961 tcgcgagcat gttcgaactg cgcaagttca gttgggttta ggtcctcata ctggcgctca
     1021 agtcgacgtt ggagcgaggg gcgaaaggtg ggggggccga gtgcgcctgg gaccttggct
     1081 agcactcgaa ctgtgtcgct tggaatcacc cagtaaaaaa actcgtcggt taagtgccag
     1141 ttcgggttgg ctacttccag aagtgaaagt aagtggtagg aacagttctc gtcgaaaaaa
     1201 aaataatcaa aataagtact gccgagttcc caaatgtgtc cgatcattcg gtgaatttgt
     1261 tcgggttcaa gatggagtgg gtattcccaa agatcgcggc tctcgacgtc ggagtattcc
     1321 tgaacttttt cgaagtaggg gagtaacgaa aaaaatcctg ggtacccacc gataaggcct
     1381 agaacggcaa aagtaattcc tccgtcgtca ccagttttcg cagaatagtt tacgccgtaa
     1441 tcgagaagat cgtttttctt ggtgtgatca attctaggtt ttgaatctat tcgaagaaac
     1501 gtatgtccaa acattgatgc cgggttattc aaataggcag aggcaaatac cagcgtagca
     1561 ccagagggtg aaaaacgggg cctccacgct tcgaagtcag gacatttgac aacaggcgta
     1621 gccaaaccca attgttcttt taaatagcgg tggcgttcgg gaaacgcaca acttggatgt
     1681 tgtttgagcg gaccaatggt ttttaccggt tccggctgca aaaacgcgcg cacattggcc
     1741 accagttctg agtaaggatc ggttcgaccg tctggtgcca gaaaaaactc tagtccgtcg
     1801 gcctcggaac gataacctcc gaac
//
LOCUS       DAOMXO010000515         1820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37945_length_1820_cov_4.938810, whole genome shotgun sequence.
ACCESSION   DAOMXO010000515 DAOMXO010000000
VERSION     DAOMXO010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37945_length_1820_cov_4.938810"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /gene="recJ"
                     /locus_tag="PLH57_09790"
     CDS             complement(<1..1633)
                     /gene="recJ"
                     /locus_tag="PLH57_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRK02943.1"
                     /translation="MESHFQSPSEIANYLNCERLTAQLLWSRGYRTVQAVEQLYNTDL
                     ANLPSPQTIVDLPKAAARIADALERKEPIAVYGDYDVDGTCGASIFFDFFRQLGSTAR
                     VYQPNRFSEGYGVHSAAIRNLIKGEGHRIVITVDCGITAVEPARVAKELGADLIIVDH
                     HKCGESLPEAFAVVDPQRPDDMSGLKTICGAGLAFFLIMAVRAELRNRDFFVRTGLRE
                     PNLLRFLDLVAVATVADVMDLRGVNRTLVTHGLKILRTNPRPGLRAILAAAGIEKPTA
                     MTCGFVIGPRINAAGRLKSAKTSFELLTTEDYGSALEFAQELEKFNLQRRETQNAVFE
                     SARQQALEILAQPLPSDLPSAVAGPWPRALVLANPEWHEGVVGIVASKLVEEFSRPVF
                     VLTESEQRPGAFKGSIRSIAKIDILSVISAQSVAQFLLNYGGHAHAGGVTLERERLED
                     FTRALNEHLSITTDQSHFTREKFFDVDAVASDITEKFLVEMDALEPFGHQFPEPVLRL
                     AAVDVQSFKVLKEKHVKLRVTGPRGVGVDAIWFNAF"
     gene            complement(1605..>1820)
                     /locus_tag="PLH57_09795"
     CDS             complement(1605..>1820)
                     /locus_tag="PLH57_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02944.1"
                     /translation="SVSTLMVVVALMLFGGGVIADFAFTMFVGMLVGTYSSIFVASTV
                     VVLMTEFQEKRARLGKGLSGKPLSKPV"
BASE COUNT          460 a          498 c          425 g          437 t
ORIGIN      
        1 caaaggcgtt aaaccaaatt gcatcgaccc ctacacctcg cgggccggtc actcgaagct
       61 taacgtgttt ctctttcaac actttaaaac tctgcacatc aactgcggcc aaccgcaaaa
      121 ctggttccgg aaactgatgc ccaaatggct ccaacgcgtc catttcgacg aggaattttt
      181 cggtaatatc ggatgccact gcgtctacgt cgaaaaattt ttctctcgtg aaatgtgact
      241 gatctgtcgt gatagaaaga tgttcgttaa gggctcgcgt aaaatcttct aagcgttcac
      301 gctcgagcgt cactcccccg gcgtgtgcgt gacctccgta gttgagtaaa aactgggcaa
      361 ccgactgagc cgagattacg gacaagatgt cgatcttcgc tatcgatcga atgcttcctt
      421 tgaaagcgcc tggccgctgt tccgattcgg tgagtacaaa cactggccgc gaaaactctt
      481 cgactagttt agacgccact atccccacca ctccctcgtg ccattcgggg tttgccaaca
      541 ccagggcccg tggccaaggc cccgccaccg cggaaggaag gtcactcggc aacggttggg
      601 ctaaaatttc tagcgcctgt tggcgcgccg attcaaacac tgcattttgg gtttcacgcc
      661 tctgcaaatt aaatttttcg agttcctgtg cgaattccaa cgccgaacca taatcttccg
      721 tagtcagtaa ctcgaacgag gttttagcac tttttaggcg acccgccgca ttgattcggg
      781 gtccaatcac aaaaccgcaa gtcatcgctg ttggtttttc aataccggct gcggccagaa
      841 ttgctcttaa tcccgggcgt ggattggttc gtaatatttt taacccgtga gtgactagcg
      901 tgcgatttac gccgcgtaag tccattacgt cagccacagt agctactgct accaggtcca
      961 gaaaccgtaa caggttcggt tcacgaagcc cggttcgtac aaaaaaatcg cggtttcgaa
     1021 gctccgcacg tactgccatg atcagaaaaa aagcgagtcc cgcaccacaa attgttttga
     1081 gacccgacat atcgtccgga cgttgaggat caaccacggc gaaagcctcg ggcaaagact
     1141 ccccgcattt atggtgatct acaattatta gatccgcgcc tagctctttt gccacccggg
     1201 ctggttcaac tgctgtgatc ccacaatcaa ctgtaattac gatgcggtgc ccttcgcctt
     1261 taattaaatt tcgaatcgca gcggagtgta caccgtatcc ttcgctgaag cggttgggtt
     1321 gataaactcg ggccgtacta ccgagctgcc gaaaaaaatc gaaaaatatt gaagcaccac
     1381 acgtgccgtc gacgtcgtaa tcaccgtaca ccgcgattgg ctctttgcgc tccaaagcat
     1441 cggcaatgcg agcggcagcc tttggcaggt cgacaatcgt ttgagggcta ggcaagtttg
     1501 ctaagtcggt attgtagagt tgttctactg cctgaacggt tcgataaccg cgactccaaa
     1561 gaagctgcgc cgttaaacgt tcgcagttaa gataattggc gatctcagac gggctttgaa
     1621 agtggctttc cactgagtcc cttacccaag cgtgcccgct tttcttgaaa ctctgtcatg
     1681 agaaccacga ctgtcgatgc aacgaagatc gaagaatagg tgcccaccaa catacctacg
     1741 aacatcgtaa acgcgaaatc ggcaataact ccgccgccaa acaacattag tgcaacgact
     1801 accattaaag ttgaaactga
//
LOCUS       DAOMXO010000516         1820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_37949_length_1820_cov_4.613031, whole genome shotgun sequence.
ACCESSION   DAOMXO010000516 DAOMXO010000000
VERSION     DAOMXO010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37949_length_1820_cov_4.613031"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLH57_09800"
     CDS             complement(<1..293)
                     /locus_tag="PLH57_09800"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRK02945.1"
                     /translation="MISFELSDEQKELQRLAREFTAKEIVPKAAHHDEKGEFPMEIAK
                     KAWELGLMNTHVPQDCGGMGLGVLDGSIVTEELAYGCTGICTAMEANHLATAPV"
     gene            complement(336..1526)
                     /locus_tag="PLH57_09805"
     CDS             complement(336..1526)
                     /locus_tag="PLH57_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02946.1"
                     /translation="MIWFIASMLYGGAFWWKARFAEPSLGETKSIQILAEFSQSTFPP
                     KIDLVRSLDDYGGHLAYYALMGRVHQLAGGDLLTMRTIHLCVVLAALFVFTLLGFHYT
                     YRNRLNPLWISLGTLLLAANPYLFEAAMRVGYIGFFLFVLLASLWALEKERTAWATLL
                     LSLGVLVDWRAVILVLVTLWTRTTGETGRFVRIEKILLLALPLVFASLPLFAWKGIVP
                     TGETREWWTFILEHRNLVRLDLWFYSIALLPIYSLFFSWSWGFRARARSMSLGAVAAA
                     LLIPMYFIFPVVPQPEPWAAPGSGNAVPLGLVDWALTEVAGPYKNLVLFVPWIVGAFL
                     FAQAILLDILERSRVLRAFILFFFLVAPVSFGVVDQGFLIVLPFVLLFGLSEALVGDE
                     GKIV"
BASE COUNT          506 a          463 c          472 g          379 t
ORIGIN      
        1 accggcgcag tcgcaaggtg attggcttcc attgcagtgc agattccggt gcaaccgtag
       61 gccagttcct cggtgacaat cgagccgtcg agaactccta ggcccatgcc gccgcagtct
      121 tgcggtacgt gagtgttcat gaggcccaac tcccacgctt tttttgcgat ctccataggg
      181 aattcgcctt tttcgtcgtg gtgagcggct ttgggaacga tttcttttgc ggtaaactct
      241 cgcgccaagc gctgcagctc tttttgttcg tcggacaatt cgaatgaaat cacgattccc
      301 cctgttggaa gtttaaacgt tcgttgcgaa gtgctttaga cgattttgcc ttcgtctcct
      361 acgagtgcct cggacaaacc gaagagcagc acgaacggaa gcacgattaa aaacccttga
      421 tcaaccaccc cgaacgaaac gggtgcgacc aggaagaaaa atagaatgaa ggcgcggagc
      481 acccgcgaac gctcaagaat atcgagcaag atcgcttgcg caaaaagaaa cgcacccact
      541 atccacggca caaaaagtac cagattttta tacggtccgg caacttctgt taatgcccaa
      601 tcgaccaatc caagcggcac cgcgttgcca cttcctgggg cggcccaagg ttcaggttga
      661 ggcacgactg gaaatataaa atacatggga attaaaagag cggcagccac ggcacccaaa
      721 ctcatcgatc gggcgcgagc ccggaatccc cagctccatg aaaaaaataa gctgtagatt
      781 ggcagaagcg cgattgagta aaaccacaag tccagacgta cgagattgcg atgttccagt
      841 atgaaggtcc accattcgcg cgtttcgccg gttggaacaa tgcctttcca cgcaaaaagc
      901 ggcaaagagg caaaaaccag cggcagggcc agcaataaaa ttttttcaat tcgtacgaag
      961 cgtccggttt cgccagtggt gcgggtccac aaggtgacga gcactaaaat cactgcacgc
     1021 caatcgacga ggacacccaa gctcaacaac aaagttgccc aggccgtgcg ttccttctcg
     1081 agggcccaga gcgaggcgag caaaacgaat agaaaaaaac caatataacc cacccgcatc
     1141 gccgcttcga agagataggg gtttgctgct aacaaaagag tgccgaggct gatccaaagc
     1201 gggttaagcc gattgcggta ggtgtagtga aaaccgagga gagtaaatac aaacaatgca
     1261 gctaaaacca cgcataaatg aattgtgcgc attgtgagta agtcgccccc ggcaagctgg
     1321 tgtacgcgcc ccatgagagc gtaataggcg agatggccgc cgtaatcgtc gagcgaacgc
     1381 acgaggtcga ttttcggagg aaaggtgctc tgcgaaaatt ccgccaatat ttgaattgat
     1441 ttcgtttccc ctaagcttgg ttctgcgaaa cgagctttcc accagaaagc tccgccatag
     1501 agcatcgatg caataaacca gatcaaaaaa ggcttctcga gaagggcctt aaaggagcgc
     1561 atgttagtta agtatcattt tttcgtattt tctgaataat aacaatgggt tataggcaaa
     1621 aattgctagg tttcccgggg ttttcgccta aaagttcgac agcgcggcgg gcaggaatcg
     1681 cccacttagc ggggtttgcc gaaccttcct gccgagtgga gtgcgaaacc ccccttcgtt
     1741 ttgcaccctt aactcaactt tgcgcgcttc ttgcacgaaa agaacagtgg gggaacaaaa
     1801 cgtggggagt agttgggaaa
//
LOCUS       DAOMXO010000517         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_38000_length_1818_cov_5.041407, whole genome shotgun sequence.
ACCESSION   DAOMXO010000517 DAOMXO010000000
VERSION     DAOMXO010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38000_length_1818_cov_5.041407"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLH57_09810"
     CDS             complement(<1..623)
                     /locus_tag="PLH57_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02947.1"
                     /translation="MGNDKNSGEPNESKYGDATFVSLPLLKISTGKPLPGAIFLCLGH
                     RMVRYRSKDDPIEPAEYDKLVFNRVRYIFFDEVDRMAFDRWVKNTEAADTALEPAVAA
                     NAEAAPIAQAAMDQRRAIMDIFSNPKDDKQVAAAVDVSKRVVTEFMRKPFAINNIQQL
                     QRYAKGAVDHSVNVSMLCVFLGLRMGYSHQVILENLALGGLFHDIGKV"
     gene            649..936
                     /locus_tag="PLH57_09815"
     CDS             649..936
                     /locus_tag="PLH57_09815"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HRK02948.1"
                     /translation="MKNLKAILNNILDSNPYYKGHKVELQILEKWPETVGARIAQHCT
                     PERFTDDGTLIVSSNNSAWLHQLKYLEPQIVTKLNKALGEDRIKQLRFRLR"
     gene            complement(958..>1818)
                     /locus_tag="PLH57_09820"
     CDS             complement(958..>1818)
                     /locus_tag="PLH57_09820"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRK02949.1"
                     /translation="SDSVTWSEGAASFYVLRAIHEGESSRWCLGVRLLSPSLLAHEKT
                     FARSVREKCRVFRRVKINPWPVLSAIDLDWKSWNSWLQESVAGAPPLVDNNYVALWRL
                     NQLADTTLKFPSKILVLDGHHSLEAQRTVLPEKPITSWVLPRNHPELHVRPIHRAGWL
                     KATIEKALQKQEITLIQGVDNKKLAFDPHVTEVIQSQKRFYVRVHKQDAIDWVGGLDN
                     ARTQLRTSPDIESLVNMLTQNECDTVLQMAQLEKKAIFDRASQGLLYPQKATYFFPKV
                     PFGWLVERLV"
BASE COUNT          463 a          481 c          433 g          441 t
ORIGIN      
        1 acctttccga tatcgtgaaa aagccccccg agcgccaagt tttccagaat aacttggtga
       61 gaataaccca ttcgaagtcc caaaaacaca caaagcatgg aaacgttcac cgaatgatcc
      121 acagcgcctt tggcgtaacg ttgaagctgt tgaatattgt taatcgcgaa gggttttcgc
      181 atgaactccg tgacgacccg tttggaaaca tctaccgcgg cggcaacctg tttgtcgtcc
      241 ttggggttgg aaaatatatc catgatcgcc cggcgctggt ccatggctgc ttgagcaatc
      301 ggcgccgctt cggcgttggc cgctactgct ggttcgaggg ccgtatcggc agcctcggtg
      361 tttttcaccc aacgatcgaa agccatgcga tcgacttcgt cgaaaaaaat ataacggacc
      421 cgattaaaga cgagtttatc gtactctgcg ggctcgatag ggtcatcttt ggatcgataa
      481 cgcaccatgc gatggccgag acacaggaaa atggctcctg gcagcggttt tcccgtagaa
      541 atttttagca gcggtaacga aacaaatgtc gcatcaccgt attttgactc atttggttca
      601 ccggagttct tgtcgttacc catctattct tctaaggtaa ccctggtaat gaagaattta
      661 aaggcgattc tcaataatat cctggattcg aacccctact acaaagggca caaggttgaa
      721 ctgcagattc tagaaaaatg gcccgaaact gtgggggctc gaatcgcgca acattgtacg
      781 cccgaacgct tcaccgacga cggaactctt attgtgtcgt ctaacaatag tgcctggctt
      841 caccaactga agtacctaga gcctcagatc gtcaccaagc tcaataaagc tctcggtgag
      901 gaccgcataa aacaactacg atttcgattg cgttagaaga tgagaagctc ggagtagcta
      961 caccaggcgt tctactaacc acccgaacgg taccttagga aaaaaataag tggccttctg
     1021 cgggtagagc aacccctgac tcgctcgatc aaaaatggct ttcttctcga gctgcgccat
     1081 ctgcaaaacc gtgtcacact cgttctgggt taacatgttc accagactct caatatccgg
     1141 actggtgcga agctgtgttc gagcattgtc gaggcccccc acccagtcaa tggcgtcttg
     1201 cttgtgcacc cggacgtaaa aacgtttttg ggactgaata acctcggtca cgtgcggatc
     1261 gaacgctagt tttttattat caacaccttg tatcaacgtg atttcttgtt tttgaagcgc
     1321 tttttcgatg gttgccttaa gccagcccgc tcggtgaatt gggcgaacgt gcaattccgg
     1381 gtgatttcgc ggcaacaccc aacttgtgat gggtttctcg ggcaaaaccg ttcgctgagc
     1441 ctccagggaa tggtgcccat cgagcaccaa gatcttcgat ggaaatttta aagtggtgtc
     1501 ggcaagctga tttaatcgcc atagtgccac gtaattattg tcaactagag gcggcgcccc
     1561 cgccacgctc tcctgaagcc aagagttcca agatttccaa tccaagtcga tagccgataa
     1621 caccggccag ggattgattt taactctccg aaacactctg cacttttctc gcactgatcg
     1681 ggcaaaggtc ttctcgtgag ccagtaaact cggtgaaagc agtcgcacgc ccagacacca
     1741 gcgactcgac tctccttcgt gaatggcgcg aagaacgtaa aaagacgccg ccccctctga
     1801 ccaagtaacg ctgtcact
//
LOCUS       DAOMXO010000518         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_38099_length_1815_cov_4.131818, whole genome shotgun sequence.
ACCESSION   DAOMXO010000518 DAOMXO010000000
VERSION     DAOMXO010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38099_length_1815_cov_4.131818"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PLH57_09825"
     CDS             <1..895
                     /locus_tag="PLH57_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02950.1"
                     /translation="LGTNFSFAITVFFAFIFSIGNGAPLRSETIDDIEEETDDIMVPE
                     FIQKKRIKQVPDGIVSEQTIETEASAKIPKKSARTVRHERRALLRKIQALSRKHQKKP
                     SKDNSSCQAFVDTFFKEQGLGSLQDLNKKALPNGILVNKSRKGEALGYFSADSKSGHT
                     FRGKMLDSDETRWQVEVIEPPRGDAHRRSLQSFEFERRGADCKLRKVTYLQTATDEYT
                     SYDSYSCKSSSDLAAEFDKGKKADRALLKSKNLTTRTAALLSACRPAYQQFFDAPQTQ
                     IVEDLFDEDETVNPLRNQSAE"
     gene            complement(956..1159)
                     /locus_tag="PLH57_09830"
     CDS             complement(956..1159)
                     /locus_tag="PLH57_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRK02951.1"
                     /translation="MAQGKVKWFNDAKGFGFISRDDGSDVFVHYSAIKGEGYRTLAEG
                     QSVEFDLYEGTKGPQAQNVTKVQ"
     gene            complement(1299..1577)
                     /locus_tag="PLH57_09835"
     CDS             complement(1299..1577)
                     /locus_tag="PLH57_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRK02952.1"
                     /translation="MIANRKFRIVVLVLFCWSVWISGLFGNDGILQAYRLSKVRRDLT
                     LRIRALENERSRLDILLSDLYRDDFAQEMAIRDTLGYVREGEVVFETR"
     gene            complement(1574..>1815)
                     /gene="eno"
                     /locus_tag="PLH57_09840"
     CDS             complement(1574..>1815)
                     /gene="eno"
                     /locus_tag="PLH57_09840"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935013.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRK02953.1"
                     /translation="TLRTIRMADAAKYTSVISHRSGETEDAFIADLAVATSAGQIKTG
                     SASRSDRLAKYNQLLRIEEQLGRRARFMGLQSLKR"
BASE COUNT          499 a          459 c          413 g          444 t
ORIGIN      
        1 actgggtacg aacttttcct ttgcgatcac agtgtttttt gcgtttattt tttctattgg
       61 aaacggcgcc ccgctacgtt cagaaactat cgacgatatt gaagaagaaa ctgacgacat
      121 aatggttccg gagtttattc aaaagaaaag aattaaacag gtgcctgacg ggattgtttc
      181 ggaacagact attgaaaccg aagcttctgc caaaatacca aaaaaatctg cccgcaccgt
      241 tcgccacgaa cgtcgggccc tcctaagaaa gatacaggct ctgagtcgca aacaccaaaa
      301 aaagccgtcc aaagacaaca gcagttgcca ggccttcgtt gatacgtttt ttaaagaaca
      361 aggtcttgga tctttacagg atctaaataa aaaagcgttg ccgaacggaa ttcttgtgaa
      421 caaaagtcgc aaaggggaag cgctcggcta tttctcggcc gattctaaat cgggccatac
      481 ctttcgaggt aaaatgttgg actccgacga aactcgttgg caggtcgaag tgatcgaacc
      541 gcctcgcggc gacgcccacc gacgatcgct ccaatctttt gagttcgagc gtcgcggagc
      601 tgactgcaag ttaagaaaag tcacctattt gcaaacagct accgacgaat acacttccta
      661 cgattcatat tcttgcaaat catcgagcga cctggctgcg gaattcgata agggcaaaaa
      721 agcagatcgg gcattactga aaagtaaaaa tctaaccacc cgcactgcgg ccctactttc
      781 tgcctgccgg ccggcctacc agcagttttt tgatgctccg cagacccaaa tagtcgaaga
      841 tctgttcgac gaagacgaaa cggtcaatcc attgcgaaat caaagcgcag aataaaaaaa
      901 agccccgcta gatcactcta gcggggccga atacttcgtt tcgctttctc aaattttatt
      961 gaaccttagt gacgttctgc gcttgaggtc cttttgtccc ctcgtaaaga tcgaactcaa
     1021 cgctctgacc ttccgctaac gtccgataac cttctccttt gatggccgaa tagtgtacga
     1081 aaacgtcgga accatcgtcg cgactaataa agccaaatcc tttggcgtcg ttaaaccact
     1141 ttacttttcc ctgtgccatg ttcgcgtgtc ctccttgaac tatgaactcg tgcggtcgca
     1201 tcgagttcag gcgatgggtc caaacccacc tgctaaaaag ttatcaaagg ggtgaaaaac
     1261 gtcaataggc cgaccttgat ctagtgagga aagacccttt agcgggtctc gaaaacgact
     1321 tcgccttcgc gtacataccc gagggtatcc ctaatggcca tttcctgggc aaaatcgtcc
     1381 cggtagaggt ccgagagtaa gatatctagc cgggagcgct cattttcgag ggctcgaatg
     1441 cgcagtgtca gatcgcggcg aacctttgat agtcgatagg cctgcaatat tccatcgtta
     1501 ccgaataggc cagaaatcca aacggaccaa cagaataaaa ctaaaaccac tatccgaaat
     1561 tttctattcg ctatcatcgc tttaacgatt gtagccccat aaatcgggcg cgtcgaccga
     1621 gttgttcttc tattcgaaga agttgattat atttcgctaa acgatcggaa cgagaggctg
     1681 atccggtttt aatttgaccc gctgaagtcg caactgcgag atcagcgatg aaggcgtctt
     1741 ccgtttctcc acttcggtgg gaaatgaccg atgtgtattt cgccgcgtcc gccatgcgaa
     1801 tcgtgcgcag cgttt
//
LOCUS       DAOMXO010000519         1813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_38153_length_1813_cov_3.740046, whole genome shotgun sequence.
ACCESSION   DAOMXO010000519 DAOMXO010000000
VERSION     DAOMXO010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38153_length_1813_cov_3.740046"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLH57_09845"
     CDS             complement(<1..618)
                     /locus_tag="PLH57_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK02954.1"
                     /translation="MKTRLLHSLLVVGIVLLQGCAGSPPKGEKESSDEVLAIARDENT
                     PDAREYQKIDSLYRARDYDGVLSKSAGFAKNHSKSPFLDMVYNLRGLALLGQKQYQNA
                     ISVFKKVLNTTERTELRNMAQYNLAFSYFELGQVDLAANALDTTKAASLDYDNQTKFY
                     ILRAKVSRLKQDNGAAARDILNAIEAGRNAEKQAAAFEPMIAFFDD"
     gene            complement(682..>1813)
                     /gene="dnaJ"
                     /locus_tag="PLH57_09850"
     CDS             complement(682..>1813)
                     /gene="dnaJ"
                     /locus_tag="PLH57_09850"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRK02955.1"
                     /translation="EILGVGKSAGPEELKKAYRKLAVQFHPDKNPGDKAAEERFKELS
                     EAYEVLSTPEKRKMYDQFGHAAANMGGGGPYGGAGGFQGFDFGGGSINDIFGDIFGDI
                     FGGGRGGAGGARRRSRAQSGADLQYELEVTFEEAAFGTEKVISIPRNSECKTCHGSGA
                     KPGTSASKCGQCHGSGEMRFQQGFFSVSRTCNRCGGTGETISDPCTDCSGSGRNRKNI
                     DLAIKIPAGIDTGQRLKLSGEGEPGLRGGPSGDLYVVIQVQDHPFFTREEADITCEVP
                     ISFPQAALGAEIDVPTLEGKAKLKIPAGMQSNKILRLKHKGIAKLGGYGRGDQLVRVL
                     VETPQKLSAEQKELLRRFQELSDEKSHPLAKGFFDKMRGLFE"
BASE COUNT          415 a          471 c          421 g          506 t
ORIGIN      
        1 atcgtcgaaa aacgcgatca ttggttcaaa ggcagctgcc tgtttttcgg catttcgccc
       61 agcttcgata gcgtttaata tgtcgcgggc tgctgcgccg ttgtcttgtt tcaggcgcga
      121 aacctttgct cttaaaatat aaaacttagt ttggttatcg taatcgaggc tggcggcttt
      181 ggtggtgtct agcgcgtttg ccgctagatc cacttgcccg agttcaaagt aagaaaatgc
      241 taagttgtac tgcgccatat tgcgaagctc ggtacgttca gtggtgttga gaaccttttt
      301 aaataccgaa atcgcatttt gatattgttt ctgcccgagc agtgccaggc cgcgcaaatt
      361 gtaaaccata tccaaaaatg gcgactttga gtggtttttg gcaaaccccg cacttttact
      421 gagcacgcca tcgtaatccc ttgcgcggta aagcgaatct attttttggt attctcgagc
      481 gtcgggggtg ttttcgtctc gagcaatggc gagtacctca tcgctcgact ctttttcgcc
      541 ctttggtggc gaaccagcac aaccttggag caatacgatt ccaaccacta aaagtgaatg
      601 taagagtcgt gttttcacgt tctctgattc tacagaacgc agagcagctt agtctaggtt
      661 aataaggtta ttgattaatg gctactcgaa cagaccgcgc atcttgtcaa aaaaaccctt
      721 ggcgagcgga tggctctttt cgtcgcttag ttcctgaaat cgacgaagca gttctttttg
      781 ctcggcagat agtttttggg gtgtttctac caatactcga actaattggt cgccccgacc
      841 gtatccacca agcttagcta tgcccttgtg tttcagcctt aaaatcttat tggactgcat
      901 gccggctggg atttttagtt tagctttacc ctcaagcgtt ggcacatcga tttcggcacc
      961 taaagcagct tgtggaaaac taatagggac ctcacacgta atgtccgcct cttctcgggt
     1021 aaagaaaggg tgatcttgca cttggataac cacgtaaagg tcgcccgaag gtcccccgcg
     1081 caaaccgggt tcaccctcgc ctgaaagttt taggcgttga cccgtatcga tacccgctgg
     1141 aatcttgatt gcgaggtcga tgttctttcg atttcttcca gaacctgaac aatccgtgca
     1201 ggggtcagaa atagtttcgc ccgttccacc gcagcgattg caggttctcg aaacagaaaa
     1261 aaatccctgt tgaaaacgca tttcacccga accatggcat tgcccacatt tcgacgcact
     1321 tgttcctggc ttggcaccag acccatgaca ggttttgcac tcactgtttc gaggaatgga
     1381 aattactttt tcagttccaa aagccgcttc ttcgaaagtg acttctagtt catattgaag
     1441 atcagcccca gattgggctc tcgagcggcg ccgcgcgcct cccgcacctc cgcggccgcc
     1501 tccaaaaata tcaccaaaga tatctccgaa aatatcgtta attgatccgc cgccgaaatc
     1561 gaaaccttga aaacccccgg ctccgccgta aggaccgccg ccacccatat tggctgcggc
     1621 gtggccgaac tgatcgtaca tttttctttt ttcgggagtg cttaacactt cgtaagcctc
     1681 ggaaagttct ttaaaacgtt cctccgcagc cttgtcgccc ggatttttgt ctgggtgaaa
     1741 ctgcactgcg agcttacgat atgctttttt gagttcctcg gggccggcgc ttttaccgac
     1801 cccgagaatc tcg
//
LOCUS       DAOMXO010000520         1805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_38397_length_1805_cov_6.488000, whole genome shotgun sequence.
ACCESSION   DAOMXO010000520 DAOMXO010000000
VERSION     DAOMXO010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38397_length_1805_cov_6.488000"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="PLH57_09855"
     CDS             <1..1548
                     /locus_tag="PLH57_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02956.1"
                     /translation="ASQAGVSLVGAMIAGPIIAAAAFALGQVLLNYQRGMKSAEFAQT
                     RNDLFSQIQRTLRDDSAVFTTLNSSSAAANVGVGKLIDDYAKCSSDANGLSDCVPRSY
                     EARTKYTLYSMAGIPASSTTLSNDRYTVNGVKCNATQISACDDGNIATACECPLIVET
                     FYQITCPAPRLPVVIMPPIVMNPGFPTPGPIATPTPTPAPVGCTKAYNFALYGAVRQD
                     PNVTLKGYQTLGERVTAPRYLISNGNVVGVMPEEEPLNHTVIANHTLNNLSPTCTFQV
                     NGNSSRTFEQLGTGYSKIGDWNTNTIGTVYDLGSPGSCGALNNDQRICTTGGCTQRNY
                     GSPTAPTALYLVSSLRAGFSNTQQIYSTTNTGYQTCLNACVHVIEANQPQCITNCETS
                     YPESTTTIENWLRPKVSRCSVCMIPGPINVVHGNAAEVPTCPTGWDSLWSGYSLLGGA
                     FQRASGGMIFEVDKESPGSCVRNYIDIPFMGTMAFRAGSETTSWGSSPDSMNASRCNV
                     CYKPASQ"
     gene            complement(1558..>1805)
                     /locus_tag="PLH57_09860"
     CDS             complement(1558..>1805)
                     /locus_tag="PLH57_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HRK02957.1"
                     /translation="NPEGEIRAIVLRHLRPWKGHDDHFHFRLLCPPTDEHCSGNSWLP
                     EGDGCDDPVLTDGFPKKTKKTEDKPKPAPHPLCAKGV"
BASE COUNT          440 a          499 c          430 g          436 t
ORIGIN      
        1 gcctcccagg cgggcgtttc gttagttggt gctatgatcg cgggcccgat tatcgcagcc
       61 gcggcttttg ctttgggcca agtactcttg aattaccaac gcggaatgaa aagcgccgaa
      121 ttcgcccaaa ctcgaaatga tctcttcagt caaattcaaa gaaccctgcg cgacgacagt
      181 gcagttttca ccactctaaa ttcttcatcg gctgctgcga acgttggggt cggtaaactc
      241 atcgacgatt acgcaaaatg ctcatctgac gccaacggcc tgtcagattg cgtccctcga
      301 tcttacgagg ctcgcaccaa atacacgctc tacagcatgg ctggaattcc ggcttcgtcc
      361 accacacttt caaacgatcg ttacactgta aacggcgtga aatgtaatgc cactcagatt
      421 tcagcctgcg acgatggaaa cattgcgacc gcatgcgaat gtccgttgat cgtggaaaca
      481 ttttatcaaa taacctgccc agctcctcgc ttgcctgtcg tcatcatgcc gccgatcgta
      541 atgaatcccg gatttccaac tcctggacca attgcgactc cgacaccgac ccccgcgccg
      601 gtaggctgta ccaaggccta taacttcgcc ctctatggtg ctgttcgaca agatcctaac
      661 gttacactta agggctacca gacattgggt gagcgagtga ccgctccccg ttatctcatc
      721 tccaatggca acgtggtcgg cgtaatgccc gaagaagaac cgctcaacca cacggtaatc
      781 gcaaatcaca ccttgaacaa tctatctcct acctgcacgt ttcaggtaaa cggaaattca
      841 agtcgaacat ttgaacagtt gggcaccggt tactcgaaaa ttggcgactg gaacacgaat
      901 actattggta cagtctatga cctcggttca ccgggctctt gtggagctct gaacaatgac
      961 cagcgaattt gcaccactgg cggctgtacg cagcgaaatt acgggtctcc gactgcgccg
     1021 acggcattgt atttggtctc ttctttgaga gccggatttt ctaacactca gcaaatctat
     1081 tcgacaacga atactggtta tcagacgtgt ttaaatgctt gcgtccacgt catcgaagcc
     1141 aatcaacctc aatgcataac aaactgcgag accagctacc cagaatccac caccacaata
     1201 gaaaactggc tcagaccgaa agttagtcga tgctcggtgt gcatgattcc gggtccaata
     1261 aatgtagtgc acggcaatgc agccgaagtt cctacctgcc caacgggctg ggattcgctg
     1321 tggtctggtt actctttgtt agggggcgca tttcaacgag cttccggcgg catgatcttc
     1381 gaagtagata aagaaagccc cggctcctgc gtaaggaact atatcgatat tccttttatg
     1441 ggcactatgg catttagagc cggttctgag accacctcat ggggatcgtc gccggatagt
     1501 atgaatgcct cgcggtgcaa cgtgtgctac aaaccagcgt ctcaatagaa attaaattta
     1561 aacgcccttt gcacacaacg gatgcggcgc gggttttggt ttatcttcag tctttttagt
     1621 ttttttcgga aacccatcgg tcagcacggg gtcgtcgcag ccgtctccct ccggcaacca
     1681 actgttgccc gagcagtgtt catcggtcgg cgggcacaag aggcgaaagt gaaaatgatc
     1741 gtcgtgcccc ttccagggcc gaaggtggcg caatacaatt gcacgtattt cgccctccgg
     1801 gttct
//
LOCUS       DAOMXO010000521         1801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_38517_length_1801_cov_5.498855, whole genome shotgun sequence.
ACCESSION   DAOMXO010000521 DAOMXO010000000
VERSION     DAOMXO010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38517_length_1801_cov_5.498855"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="PLH57_09865"
     CDS             complement(<1..1266)
                     /locus_tag="PLH57_09865"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRK02958.1"
                     /translation="MKVLFRQWISGLQRFRVQALALGAGLVLTLVLQNFQFNLFEAYL
                     YDLRMRNKGTASTPNHVAIIKIDDKTVEQLNEFAPLSIEQHVLLLRILGRAKPKAVAY
                     LINFNDSMLADGDDHSNPRVMRAADSFVDLADQLSTNGTQVILGTEIDVTGEVVPAFP
                     LSKLPHRVALLHKDGNTFSEDKVTRRALFSVYDEPVLHTHLAGIISGKTPQTKDFRGV
                     YYMPEVEAHYFYINYSGPTQDDRHGIPEYSAIDVLNGSVAADAFENKVVLIGTKTKDD
                     SNDYVYTPYSRAIFTNSKLVVHANVLETLVNNNAIVKASSLVDFALTFLLTSFIIGLV
                     FYTTPARGVAVTIICAIGVTIFASIAFRFFSVWVRLAHPLLGVFFAYYVFVPYRLIME
                     YKKRWQFQKKNEVLLQVEELKNNFMGLITH"
     gene            1435..>1801
                     /locus_tag="PLH57_09870"
     CDS             1435..>1801
                     /locus_tag="PLH57_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRK02959.1"
                     /translation="MGGIAVGGKVPEFTLKDQNGKDQSFYKDILPKGAALVVFYPGDF
                     TMVCTKQLCSYRDNLDEFKNLGVQVVGVSKNSTKDHESFSKEYGFNFSLLSDDKREVA
                     KEFGTASFLSIGGVSRAVFI"
BASE COUNT          477 a          400 c          458 g          466 t
ORIGIN      
        1 gtgggtgata agacccatga agttgttctt cagttcttca acttgcagca atacttcgtt
       61 tttcttttga aactgccagc gttttttgta ctccattatc aagcggtatg gaacaaagac
      121 gtaatacgca aaaaatacgc caagcaacgg gtgggccaat cgaacccaca cggaaaaaaa
      181 ccgaaacgcg atcgatgcaa aaatggttac cccgatagcg caaattatag tgactgccac
      241 cccgcgtgcg ggagtggtgt aaaaaactaa tccaataata aagctcgtga gtaaaaatgt
      301 taacgcgaag tcgaccaggc tagaagcttt cacgatcgcg ttgttgttta ccaaagtttc
      361 taaaacgttc gcgtgaacga ctagttttga attcgtaaaa atcgcgcgag aatacggcgt
      421 atacacatag tcattcgagt cgtcttttgt cttcgtgcct attaatacta ctttgttctc
      481 aaacgcatcg gccgcaacac tgccattcag tacatcaatg gctgaatact cgggtatccc
      541 gtgccggtca tcttgggtcg gacccgaata atttatataa aagtagtggg cctcaacttc
      601 aggcatgtag tagacgccac gaaaatcttt ggtttgaggt gttttgccag aaatgattcc
      661 cgccaaatga gtgtggagaa ccggctcgtc gtacaccgaa aatagcgccc gacgtgtaac
      721 tttatcttcc gaaaaagtgt ttccgtcttt gtgaagtagc gctactcgat gcggcagttt
      781 actgagcggg aacgctggca ccacttcgcc ggtaacgtcg atttcggtac ccaaaatgac
      841 ctgggtgcca ttggttgaaa gttggtcggc aaggtccacg aatgaatctg ccgcacgcat
      901 gacgcgaggg tttgaatggt catcaccgtc ggcgagcatg ctgtcgttaa aattaatgag
      961 gtaagccacc gcttttggtt ttgctcggcc aagtatgcga agcagcaaca catgttgttc
     1021 gatcgaaagc ggcgcaaact cgttaagttg ttctacggtt ttatcgtcaa tcttgatgat
     1081 cgctacgtgg ttgggcgtag aggccgtgcc cttgtttctc atgcgaaggt cgtacaggta
     1141 ggcctcaaag agattgaact gaaagttttg cagtaccagt gttagaacaa gcccagctcc
     1201 gagtgctaga gcctgcaccc ggaatctttg aagaccacta atccactgcc tgaagagtac
     1261 tttcatagtc gccggccgcg aaagcgggaa cggcagaacc ctctcagcaa ggggtatgcc
     1321 attttgacac cagtctgaca atcgtgattt caaggagtta gccctgaggt tagatctgcg
     1381 gccttgtcga agtcttgctc accgagtagt ttttacagca attcggaggt tggtatgggt
     1441 ggaatcgcag ttggcggtaa agtgccggaa ttcacgctga aagatcaaaa cggcaaggat
     1501 caatcgttct ataaagacat tctacctaag ggtgccgcgt tggtggtgtt ttatccgggc
     1561 gactttacaa tggtttgcac caaacaactc tgtagttatc gcgataacct agatgaattt
     1621 aagaacctag gcgtgcaggt cgtcggagtg agcaagaatt ccacgaaaga ccacgaaagt
     1681 ttttcgaagg aatacggttt taatttttcg ctactgagcg acgataagcg cgaagtcgcc
     1741 aaggaatttg gcacggcctc gtttttaagt attggtggag tgagccgtgc agtgttcatt
     1801 g
//
LOCUS       DAOMXO010000522         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_38550_length_1800_cov_4.432092, whole genome shotgun sequence.
ACCESSION   DAOMXO010000522 DAOMXO010000000
VERSION     DAOMXO010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38550_length_1800_cov_4.432092"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLH57_09875"
     CDS             <1..528
                     /locus_tag="PLH57_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02960.1"
                     /translation="FFIFIFIAILGVTVQSTVFSYFPIHYLQPDLLLIMCVYAGFHRN
                     NIEGGIFAILCAMIAENNSTAAESYSLVVYVFVFVISKILSRAVVVPDLMTSTGIVAG
                     LSLFKKLVLLALLAHAGKFWNSFESFLIYLIPGVAVQAALTPVVFTWLNRVDLRTYKD
                     SHADDEYQFSNRELK"
     gene            530..>1800
                     /locus_tag="PLH57_09880"
     CDS             530..>1800
                     /locus_tag="PLH57_09880"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRK02961.1"
                     /translation="MSFLGQDEQIREYQFRFRYLYIALFAAFIILIARLWYLQIISGD
                     KFRQYSEENRIKRVKIPSPRGMIFDRNKKLLVDNRPAFDLEITPQELYADKEWKLTVA
                     RLSKMTKIPVDEILKTLAAGKGQPRFLPIKIKQDLSNEEVAIVEAHKLDLPGVNIQME
                     IKRTNLYNDLAAHLMGYIGEVNQADLIRLNKALAPGAVPYKMGDSTGKFGLEARWEHT
                     LRGIDGEEYIEVDALGRRKKITKGLATELKAKPSVPGQNLVLTIDQDLQIKAAEQLNK
                     LHGEMKIGAVIALNPKNGEILAAVSQPSFDPTLFSRGVSTKVWNELLNNEFHPLRDKT
                     LQDHYSPGSTFKVVTAIAGLEEKEIDENTTVHCPGSYRLGNRTYRCHKRSGHGIVNLS
                     SAIAVSCDVYFYRLGQKLGIDRIAKYAKMLGL"
BASE COUNT          510 a          411 c          409 g          470 t
ORIGIN      
        1 ttttttattt ttatatttat cgccatattg ggcgtaaccg tgcaatcgac ggtattctcc
       61 tattttccaa ttcattactt gcagcccgac cttttgctga tcatgtgtgt ttatgccggg
      121 tttcaccgga acaacattga aggtggaata tttgcgattt tgtgcgcgat gattgccgag
      181 aacaacagca ctgcggcaga gagttattct ctggttgttt acgtttttgt attcgtaata
      241 tcgaaaatat taagtcgcgc cgttgtggtg cctgatctca tgacgagcac gggcatcgtc
      301 gctggtcttt cattgtttaa aaaactcgtt ttactcgctt tgttggccca cgctggaaaa
      361 ttttggaact cgttcgagag ttttttgatc tacttaattc cgggagtcgc ggtacaggcc
      421 gctctcactc cagtggtgtt tacctggctg aaccgagtgg atctgcgtac ctacaaagat
      481 tcccatgccg acgacgaata tcagttttcc aacagggagc taaaataacg tgtcgtttct
      541 cgggcaagac gaacagatta gagagtacca gtttaggttt cggtacctgt acattgcctt
      601 gttcgcagcc ttcattatac tgatcgctcg gctttggtat ttacagatta ttagtggcga
      661 caagtttcgc cagtactccg aagaaaatcg aataaaacga gtaaaaatac ctagccctag
      721 aggtatgatt ttcgatcgaa acaagaaact attggtggac aatcgtccgg cctttgatct
      781 ggaaattacc ccccaagaac tttacgccga caaagagtgg aaactcacag tggcgcgcct
      841 ttcgaaaatg accaaaatac cggtcgacga aatactcaag accctcgccg ctggaaaggg
      901 ccagcctaga tttttgccga ttaaaattaa acaggatctt tcgaatgaag aagtagcaat
      961 cgttgaagcg cacaaactag acttgccggg cgttaacatt caaatggaaa ttaaacgcac
     1021 caatctctac aacgatctag ccgcccatct catgggatac atcggtgaag tcaatcaggc
     1081 tgacttgatt cggttaaaca aagcgctggc ccccggcgcc gtaccttaca aaatgggaga
     1141 cagcactgga aagtttggac tcgaggcgcg ctgggaacac actcttcgag gaattgatgg
     1201 agaggaatac attgaagttg atgcgctcgg aagaagaaag aaaatcacca agggattagc
     1261 taccgaactc aaagcgaaac caagtgttcc aggccaaaac ctggtattaa cgattgatca
     1321 agacctgcaa atcaaggccg ccgagcagct caataagttg cacggagaaa tgaaaatcgg
     1381 cgccgtaatc gctttaaatc ccaaaaacgg cgaaattcta gctgctgtta gccaacctag
     1441 ctttgatccg acactttttt ctcgtggcgt atctactaaa gtatggaacg aactattgaa
     1501 caacgaattt cacccgcttc gagataagac attgcaggat cactacagtc ctggctcgac
     1561 tttcaaagta gtcactgcga ttgccggttt agaagaaaaa gaaatcgacg aaaacaccac
     1621 cgtacattgt ccaggaagtt accgtcttgg aaaccgaacc taccgttgcc ataaaaggtc
     1681 gggtcacgga attgtcaatt taagttctgc cattgctgtg tcttgcgacg tctattttta
     1741 tcgactaggc caaaaactcg gcatagatcg catcgccaag tacgccaaaa tgttgggcct
//
LOCUS       DAOMXO010000523         1799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_38582_length_1799_cov_4.458716, whole genome shotgun sequence.
ACCESSION   DAOMXO010000523 DAOMXO010000000
VERSION     DAOMXO010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38582_length_1799_cov_4.458716"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="PLH57_09885"
     CDS             <1..1015
                     /locus_tag="PLH57_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02962.1"
                     /translation="VRLNPYLERASKMLTERFDALGYSWNHFVKENKSRDGSWTVGSF
                     LRVHEKIMAEISEEARRLVVEVQLLLRIQVLDSPDEAEKLIPREFRRSIKDARQDLFL
                     NYLQAQLLAASFALEKPHFTRRMRFLNATSWLQPAAVAGGLLVVGANPVLLVAAGATA
                     LQTGTQWIISEYLSDAKHSARNDGSDRIDAALEKRKIAPWLNRALNAVVAPHLTLTAL
                     LLEPHSDKNLLNTSERFLRRNYFTKRLRKQLDASPHEAAVRLLEVAGAGDTARKLLTG
                     ADPDEDPEAQTPNNVETVNCRNLLLELHQQIQSSIPLRIPFDTRKLPVLPTPKKLPSG
                     QDE"
     gene            1067..>1799
                     /locus_tag="PLH57_09890"
     CDS             1067..>1799
                     /locus_tag="PLH57_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRK02963.1"
                     /translation="MRNSSNTFATMFDHLTERLAGTFKKLRGGSVLSAAHVDDAIKEI
                     RRSLLEADVHFKVVRDVCARISERAVGQKVWDSLNPGQQVVQIFHDELVELLGGKNQQ
                     LPDFAGKPPVVVLLVGLQGSGKTTFAAKLALYLKKKLKKSVGLVPADCQRPAAKTQLQ
                     ILGQRIDTPVFDSALEKGAVAVAREGLAWAAAQFQDVVIIDTAGRQQVDDELMQEIQA
                     LNHAVVPGFRFLVVDAMIGSQGLEVA"
BASE COUNT          454 a          435 c          477 g          433 t
ORIGIN      
        1 agtccggttg aatccctatt tagagcgagc gtcgaaaatg ctcacggaaa gatttgatgc
       61 tctagggtat tcgtggaatc actttgtaaa agaaaataaa agtcgcgatg gcagttggac
      121 cgtcggtagt tttttgcgag ttcacgaaaa aataatggcg gagatttccg aagaagctcg
      181 gcgccttgtt gtagaagtcc aactcttgtt gcgcattcaa gttttggact ctcccgatga
      241 ggccgaaaaa ctgattccga gggagtttcg aagatcgata aaagatgccc gccaagattt
      301 gtttttaaac tatctacagg ctcaattgtt ggcggcttcg tttgccttag agaagcctca
      361 ctttactcgt cggatgcgat ttttaaacgc aacctcatgg ttgcaaccag cggcggtagc
      421 ggggggcctt ctggtggtcg gtgctaatcc ggttctattg gtggccgccg gtgcgaccgc
      481 gcttcagaca ggcacacagt ggatcatttc tgagtacctt tccgatgcaa aacatagtgc
      541 caggaatgac ggaagtgatc gtatcgacgc ggctctcgag aaacgaaaaa tcgccccctg
      601 gttaaatcgc gccttaaatg cggtggtcgc accgcattta acgttaaccg cgcttttgct
      661 ggagccgcac tccgataaaa acctactgaa cacatcggag cggtttttaa gacgcaatta
      721 cttcactaag cggctgcgaa aacaactaga cgcatctccc catgaggcgg cggtgcggtt
      781 acttgaagtt gctggggcgg gcgacactgc tcgaaaatta ttgacgggtg cggaccctga
      841 tgaggaccca gaagcccaaa ccccaaataa tgtcgaaact gtgaattgtc gcaacttact
      901 gctcgagctg catcagcaga ttcaaagtag tattcctttg cgaataccct ttgatacccg
      961 caaacttcca gtgttaccaa cccccaagaa actcccctcg ggacaagacg aatagaaatg
     1021 gaaacagggc ttaagttcgc cctttgcgtc tggccgctgg actcctttgc gaaattcctc
     1081 taatactttt gccaccatgt ttgatcatct tactgaacgt ttagctggca cctttaaaaa
     1141 actccgaggc gggtccgtac tttcggctgc acacgtagac gatgcaataa aagaaattcg
     1201 aagatcgctg ctcgaagccg acgtgcactt taaagtcgtg cgcgatgttt gtgcccgaat
     1261 ttcggagcgg gcggtcggtc aaaaagtgtg ggactcgctc aatcccggtc aacaagtggt
     1321 tcaaatcttt cacgacgagc ttgtcgagtt gctcggcggt aaaaatcagc agcttccaga
     1381 ttttgccgga aaacctccgg tcgtagtgct tcttgttgga ttgcaaggct caggcaaaac
     1441 gacatttgct gcaaagttag cgctttattt aaagaaaaaa ttaaaaaaat cagtgggcct
     1501 ggtgccggcg gactgccagc gaccagccgc gaaaactcag ttgcaaatac tggggcagcg
     1561 catcgataca ccggtatttg attctgctct tgaaaaaggg gccgtcgcgg ttgcacgcga
     1621 ggggcttgct tgggcggcgg cgcaattcca agacgtggtg atcatcgaca ccgccggtag
     1681 gcaacaggtc gacgatgaac tcatgcagga aattcaagct ctaaaccacg cggtggtacc
     1741 ggggttccgt tttctggtgg tggacgcaat gataggctcc caggggcttg aagtggctc
//
LOCUS       DAOMXO010000524         1798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_38614_length_1798_cov_3.476764, whole genome shotgun sequence.
ACCESSION   DAOMXO010000524 DAOMXO010000000
VERSION     DAOMXO010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38614_length_1798_cov_3.476764"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLH57_09895"
     CDS             complement(<1..643)
                     /locus_tag="PLH57_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HRK02964.1"
                     /translation="MPTTSPKFILGISAYYHDSAAALLKDGQIIAAAQEERFTRKKHD
                     ASFPKAAIQFCLDRAGIRLDSVDHVVFYDKPLLKFERLLETYLANAPRGLRSFVTAMP
                     VWLKEKLYLKTVLKKEFAAFLEKDAPKIKILFSEHHQSHAASAFYPSPYSKAAVLCLD
                     GVGEWATTSAWLGDGAKLTPLWQINFPHSLGLLYSAFTYYTGFKVNSGEYKVMG"
     gene            770..1513
                     /locus_tag="PLH57_09900"
     CDS             770..1513
                     /locus_tag="PLH57_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02965.1"
                     /translation="MKIKLLFIVGVGLLGCGLPSHAGWLNVAQTLTRDLDFGRTFDGR
                     VILSELSTAREEKMERLMNEQADAAVRLSRGGPLRRWNARRHWLNATLQLAEIDGERA
                     AEWMISRDLKVQYLLSYAWWAEARRLGVPAPGAPVQWRPGEVSGYFERNKNDLITLFG
                     EYDFYIDLKEQKDREVPHSLKKQLRKRINAFVVGLERGLNQPLPHDFEGQLRLVHQLH
                     RRLIYGASMPCGLLLIETEKVARNILKNP"
     gene            1527..>1798
                     /locus_tag="PLH57_09905"
     CDS             1527..>1798
                     /locus_tag="PLH57_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02966.1"
                     /translation="MFKRPYLLLAFCFSMKALAASQGETSACVDRMLRSPSIRSPYLD
                     IYLPFLKEITEISPELMAETNWKWSSRSDLETLEQRFRERLEGKSP"
BASE COUNT          473 a          381 c          480 g          464 t
ORIGIN      
        1 gtcccataac cttgtattcg ccggaattaa ctttgaaccc tgtgtaataa gtgaacgccg
       61 agtaaagaag tcccaaggag tggggaaaat taatttgcca aagcggggtc aacttggctc
      121 cgtccccgag ccaggcagag gtagtggccc actcgccgac tccatccaag cataatacgg
      181 cggctttcga gtaagggctg ggataaaacg ctgaagcggc atgcgattga tggtgttctg
      241 aaaagagaat ttttattttt ggggcgtcct tttcgagaaa ggctgcgaat tcttttttta
      301 aaacagtttt taaatagagt ttttccttca gccacacagg cattgcggtg acaaaagatc
      361 gaagccctcg aggcgcattc gcgaggtagg tttcgagcag acgttcaaat tttaaaagcg
      421 gtttatcgta aaacaccacg tgatcgacag aatctaaccg aataccggcg cgatctagac
      481 aaaactgaat ggctgctttt ggaaaagaag cgtcgtgttt ttttcgagtg aatcgttctt
      541 cctgcgcggc tgcaataatc tgcccgtctt taagaagagc agcggctgaa tcatggtaat
      601 aagcagagat tcccaaaatg aattttgggg acgttgtagg catatagtcg tttgataaca
      661 caccggattt gaaaggcaag attgcagatt gacgcacgcg taaggagtgt tttagttagc
      721 caaatcagga agggttttcg tgtgcatgtt atataagggg gagcaagaat tgaaaattaa
      781 actcttgttc atagtgggcg tcggtctctt ggggtgtggg ttaccatcac acgcagggtg
      841 gcttaatgtg gcgcagacgt tgactcgcga tctcgatttt ggtcgaacgt tcgacgggcg
      901 agttattttg agtgaactat ccactgctcg tgaagaaaaa atggaacgcc tgatgaatga
      961 acaggccgat gcagcagtgc ggctaagccg aggtgggccg ctgcggcgct ggaacgctcg
     1021 aaggcattgg ctgaacgcga ctttgcagct cgcggaaatt gacggcgaac gtgccgctga
     1081 gtggatgatt tcgcgggatt taaaggtcca gtatcttttg tcttacgcct ggtgggcaga
     1141 ggcacgtcgt ctaggtgttc cagcgccggg ggctccggtg caatggaggc ctggtgaggt
     1201 gtctggttat ttcgagcgaa acaaaaacga tctaattact ttgttcgggg aatatgactt
     1261 ttacattgat ctcaaagagc aaaaggatcg tgaagtccca cattcattaa agaaacagct
     1321 acgtaaacga atcaacgcgt tcgtcgtagg gctcgagcga ggcctcaacc aacctttgcc
     1381 acacgatttt gagggtcaat tgcgcttagt gcatcagctg catcgtcgac tcatatacgg
     1441 agcgtcgatg ccctgtggat tactgcttat tgagaccgaa aaagttgctc gaaatattct
     1501 aaaaaatcct taaatgggaa tcagaaatgt ttaagcgccc ctacttgctt ttggcgtttt
     1561 gtttttcgat gaaggcctta gcagcgtctc agggtgaaac gagtgcttgt gtcgatcgaa
     1621 tgctgcgctc tccaagtata cgttcacctt acctagatat ttacctccca ttcctaaaag
     1681 aaataaccga gatttcgccg gaactaatgg ccgaaacaaa ctggaagtgg tcatcgcggt
     1741 ccgatttaga aacgctcgag caacgttttc gcgaacgtct tgaaggaaaa tccccctt
//
LOCUS       DAOMXO010000525         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_38734_length_1794_cov_4.396780, whole genome shotgun sequence.
ACCESSION   DAOMXO010000525 DAOMXO010000000
VERSION     DAOMXO010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38734_length_1794_cov_4.396780"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PLH57_09910"
     CDS             complement(<1..1119)
                     /locus_tag="PLH57_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRK02967.1"
                     /translation="MVSAVRFLAFQAIEKIMDAPGVGDWDEGVHLEGPDQALFHDIVQ
                     GTLRNWRFLEKCVKSHCTRPPKRNMTYVLYTSAYQIIFYDRVPEYAVFAEAKKVAQRI
                     GFSPDEERFLSAVLKRIAENKSNYRDVHEKTLERLRLGGEFQDGDLDILNVSDDLLNL
                     LKKGPKGSFKTVVQCLVQMKKIPAVVGIELPSVTGDKPFEDTVLKKDSAIAPLLSEYR
                     GSSVDGSIFRVQSETSQFSCNLFAKEIKSAFQKGTKKIRILDVAAGKGGKLFGTLLHL
                     YGDANNLGPLEWVATDRSERQLRMLRSEWENAKKNWAVAFDKLEIKSVVHDWKQPFSS
                     DESFDFIWLDAPCTGFGTLAKHPEIALLRGSQPQVAVKE"
     gene            complement(1113..1628)
                     /locus_tag="PLH57_09915"
     CDS             complement(1113..1628)
                     /locus_tag="PLH57_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02968.1"
                     /translation="MQLLLNKLLILVAVSLALAPKEAVSASGELELVSLGPSVTVLAN
                     LEHRVLRKKDGTRAHELAEIYMLLNRCRELRKLAIDAEKKVTQLASLCDEKLESKVDP
                     EDRSELAQYLAFRSHANPGPKEFSVVSHMPEARYVYMKRLGDLRTRGRSTAPQNMFYE
                     ELVKEFLETSW"
     gene            complement(1609..>1794)
                     /locus_tag="PLH57_09920"
     CDS             complement(1609..>1794)
                     /locus_tag="PLH57_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02969.1"
                     /translation="MLTQESPDDEASAGLSGTVKDLLSLARIASRALTGEASSFEHDA
                     SSMWGPQFIISNAAFTQ"
BASE COUNT          470 a          451 c          383 g          490 t
ORIGIN      
        1 ttctttgacc gctacctgcg gttggcttcc ccgcaaaagc gctatttccg ggtgtttagc
       61 cagtgtcccg aaaccggtac atggcgcatc gagccagatg aagtcaaaag attcgtcgct
      121 actaaacggt tgcttccaat cgtgaacaac actcttaatt tcgagtttat caaatgcgac
      181 ggcccagttt tttttggcat tttcccactc agagcgtaac attctaagtt gtcgttctga
      241 acgatcggta gccacccact cgagaggccc aagattatta gcatccccat acaaatgtaa
      301 aagagtgcca aacaattttc cgccctttcc ggcagccaca tcaagaatac gaattttttt
      361 tgtccctttt tggaaggcgc tcttaatttc ttttgcaaat aggttacaag aaaactggct
      421 tgtttcactc tgaacccgaa aaatcgatcc atccacacta gatccgcgat actcactaag
      481 aaggggcgcg atggcggaat ccttttttaa cacagtgtct tcgaacggtt tgtcgccggt
      541 cactgatggc agctcaatac caacgacggc tgggatcttt ttcatttgaa ccaaacattg
      601 aaccactgtt ttaaatgaac ccttcggccc tttctttaaa agatttaaca ggtcatcact
      661 cacattgagt atatcgaggt caccgtcttg gaactcgcct ccgagccgca atctttcgag
      721 cgttttttcg tgaacgtctc gatagttact tttgttctct gcaatgcgtt ttagtaccgc
      781 actaagaaaa cgttcttcat cgggtgaaaa tccgattcgt tgcgctactt ttttagcttc
      841 agcgaatacc gcgtattcgg gaacccggtc ataaaaaatg atctgataag ccgaagtgta
      901 aagtacgtag gtcatgtttc gtttaggtgg tctggtgcaa tgacttttta cgcatttttc
      961 aagaaagcgc cagttacgca aagtgccctg cactatatca tggaagagcg cctgatcagg
     1021 accttcgaga tgaactccct catcccaatc accgacgccg ggggcgtcca taatcttctc
     1081 aatcgcttga aatgcaagaa atcgaaccgc acttaccaag aagtctccaa gaattctttg
     1141 accaattcct cataaaacat attctgaggc gcggtagaac gaccacgagt tcgtagatct
     1201 ccgagacgct tcatgtaaac atagcgggct tcgggcatgt gactaacgac cgaaaattct
     1261 ttgggacccg ggtttgcgtg cgatcgaaaa gccaaatatt gtgctaattc tgatcggtct
     1321 tcgggatcaa ctttggattc taacttttcg tcacacaacg aagctaactg cgtcactttt
     1381 ttttctgcgt cgatcgctag ttttcgcagt tcccgacatc gattgagaag catataaatt
     1441 tccgctaact cgtgggcgcg agtgccatct ttttttcgca gcacacgatg ttcaagattc
     1501 gccaatacgg taaccgaggg tccgagtgag accaactcta attcgccgct agcactcacg
     1561 gcttccttcg gcgccaacgc gagcgaaacg gcaactaaga ttaataattt attgagtaaa
     1621 agctgcattc gaaataatga attgagggcc ccacatcgac gaagcatcat gttcgaagga
     1681 agaagcctct ccggttaatg ctcgactggc aattcgcgcc aaggacaaca aatccttaac
     1741 ggtgccggac agacccgccg aagcttcatc gtctggactt tcttgagtta acat
//
LOCUS       DAOMXO010000526         1784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_39042_length_1784_cov_5.199537, whole genome shotgun sequence.
ACCESSION   DAOMXO010000526 DAOMXO010000000
VERSION     DAOMXO010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39042_length_1784_cov_5.199537"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLH57_09925"
     CDS             complement(<1..460)
                     /locus_tag="PLH57_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02970.1"
                     /translation="MKIYPFIKKTALFLFVSLGGAGSIWATPYRELAESEKIETTRIL
                     IFEIGELEKTQRELDTQISKLDITINETSPSLEYLRGEIRKAENLMKNFDIQEVYARS
                     RRIIADSQEKIKKLNELFEQASKEKNTFVLEKKRVALQISDKQKQLEAVLT"
     gene            complement(487..1425)
                     /locus_tag="PLH57_09930"
     CDS             complement(487..1425)
                     /locus_tag="PLH57_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRK02971.1"
                     /translation="MKKFMALLVVLAVTYSGLLRGEDLPGVGIVVVVDESGSMDGSKL
                     ERAKDAVEKTIALIKKTKKDQPDKNIYFWVVPFSTNARDSVYTFRNKEVKAFNRNSIE
                     IAGGTAIGRGLEKARAILDKESQLYARHIILLTDGENTDGPSPVSILEGFQERKIPIF
                     TTVIPYEMAGGALSDFKKYANVIAATESSIESKLTAITEAALAESGGAGESVKLYDPC
                     ELTFEVNMPTDIPSDSILRAFNVEKLLSPKIKSGEDALSRFVEKHKIVSFADYKRNMS
                     DRDVTDLLYRSCSKEKAGEPTTACLNQVCNDLYYAP"
     gene            complement(1567..>1784)
                     /locus_tag="PLH57_09935"
     CDS             complement(1567..>1784)
                     /locus_tag="PLH57_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02972.1"
                     /translation="LESEVSLRSEEPEATSVVGRARSPAPKKTRKTEEAKANLKLGNE
                     NKGSNASERPRWIAKRSSAANRAGCPI"
BASE COUNT          389 a          454 c          403 g          538 t
ORIGIN      
        1 ttgtaaggac cgcttccagt tgtttctgtt tatcgctaat ttgaagtgca actcgctttt
       61 tttctaatac gaatgtattt ttttctttag aagcttgttc gaaaagttca ttgagcttct
      121 taattttttc ttgcgaatcc gcgataattc gcctcgagcg agcataaacc tcttgaatgt
      181 cgaagttctt cattaagttt tcggcttttc ggatttcgcc gcgcaaatat tccaggctcg
      241 gtgaagtctc gtttatggtg atgtcgagtt tagatatctg agtatcgagt tcgcgctgtg
      301 ttttttctag ttcgccaatt tcaaaaatta aaatccgagt tgtctcgatt ttttcactct
      361 cagcaagttc gcgatacggc gtggcccaaa tagaccctgc accccctagg ctaacaaaca
      421 gaaagagcgc agttttttta atgaatgggt aaattttcaa ctcacctgcc ctcgacacag
      481 cgttcactag ggcgcataat aaaggtcgtt acacacctgg ttaaggcaag cggtcgtagg
      541 ctctcccgct ttttccttag aacaactgcg gtagagtaag tccgttacgt ccctgtcgga
      601 catgtttctt ttgtagtcgg caaatgaaac aatcttatgc ttttcaacaa aacgggaaag
      661 tgcgtcttcg ccgcttttta ttttcggcga cagcagtttt tctacgttaa acgcgcgcag
      721 aatggagtcc gaaggaatgt cggttggcat attcacttca aaagtgagtt cacacggatc
      781 gtacagcttc acggactcgc cagccccacc cgactcagct aaggccgctt cggtgatggc
      841 agttagtttg gattcgatcg aactttcggt tgctgcaatt acgttggcgt attttttaaa
      901 atcagaaagc gcgccgccgg ccatttcgta gggaataaca gtggtgaaga tcggaatttt
      961 ccgctcttga aacccctcca atatactcac gggcgaagga ccgtcggtgt tttcgccatc
     1021 agttagcaag ataatatggc gtgcgtatag ctgagattct ttgtccaaaa tggctcgggc
     1081 tttctctagc cctcgaccaa tagcggtgcc accggcgatt tcaatggagt ttcgattgaa
     1141 agcttttact tctttgtttc taaaagtgta aaccgagtcg cgagcgttgg tcgaaaaggg
     1201 caccacccag aaatagatgt ttttgtcggg ttggtctttt ttggtctttt ttatcaaagc
     1261 gatcgttttc tctaccgcgt cttttgcgcg ctccagtttg cttccgtcca tactaccgga
     1321 ctcgtcgacc acaacaacaa ttcccacccc tggcagatcc tcgccccgca ggagacccga
     1381 atacgtaaca gccaacacca ccaacagcgc catgaatttt ttcatggccg gttacttaat
     1441 atagccgatc acctaagtca atttagccac tgttttctaa tggaagggag tttggagcgg
     1501 gtgaacgggc tcgacctact acgctcgtcg cctccggctc ctcgctgcgt aggctcacct
     1561 cgctctctaa atcgggcatc ctgcccgatt agccgcgcta gatcgcttcg cgatccatcg
     1621 cggccgttcg ctcgcgttcg agcccttatt ctcattaccg agtttaaggt tcgcttttgc
     1681 ttcttctgtt tttcttgttt tttttggagc gggtgaacgg gctcgaccca ctacgctcgt
     1741 cgcctccggc tcctcgctgc gtaggctcac ctcgctctct aaat
//
LOCUS       DAOMXO010000527         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_39085_length_1783_cov_4.065394, whole genome shotgun sequence.
ACCESSION   DAOMXO010000527 DAOMXO010000000
VERSION     DAOMXO010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39085_length_1783_cov_4.065394"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PLH57_09940"
     CDS             complement(<1..1399)
                     /locus_tag="PLH57_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02973.1"
                     /translation="MLIWNIITSLVFTMLIVLSVLGTGGWIGRFSSKLGGSTSSYDDV
                     GTEVNLVLALGVFCQNVLILGLLGLYRSWVLIPFLLFSIFGIRHSRFNFKIKGPVWWL
                     VFPGLYFLARFVSVSIPHQHSDPLYYHVYAAKFWASLGAIRLESAHPSFAQATFIEPL
                     YGIYHATFGRIAATHSLFHDISGLLTAQQIHLWLGHALVVLLGARVIAQIRGSSVNTR
                     DLFFAWLASVPYFFEWSSAVAKTDYFLALFTLATFAALLSKSWFLVGLFLGFAYATKV
                     FAFWTLIALPIVLPPRHWHRAAVGFLCGMGPVLLRNLVFLKNPMFPALDSVLGPGWIS
                     QKWAQSNAGFAGPPAWPTSFHWYQKQVFAKPISVLMLAVFVLAPLLFVRVKVRAYARW
                     WLFLFAQIVVGVLILKPTAEGRYFGPVAICAALFGFAIILERVKSKKAWTFALCGLGL
                     MVNIPVDVFYKIPTRY"
     gene            complement(1409..>1783)
                     /locus_tag="PLH57_09945"
     CDS             complement(1409..>1783)
                     /locus_tag="PLH57_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02974.1"
                     /translation="EIEYWTPKQLEKCFGKHRGQYFAVFAVPTNAYAEKYLKDSGPMG
                     LVHAMIMRSGDKEQNTTIIHASTSAGQVLVEPASSFFEKMKSLHKGYAIYSFDEAWDF
                     TQVQPFAELSAITECESSLMRN"
BASE COUNT          557 a          444 c          399 g          383 t
ORIGIN      
        1 aataacgcgt cggaatctta taaaacacgt caacaggaat attgaccatg agcccaagtc
       61 cgcaaagtgc aaacgtccaa gccttttttg atttcacccg ttctagaatt attgcaaagc
      121 caaaaagtgc agcacaaata gctaccggac caaaatacct tccttcggcc gttggcttta
      181 agattaaaac tcctactacg atttgcgcaa aaagaaacag ccaccagcgc gcataggccc
      241 tgaccttaac gcgtacaaac aataatggag cgagaacaaa gaccgccagc atcaacacgc
      301 taatcggttt ggcgaacact tgtttttggt accagtgaaa actagtaggc catgctgggg
      361 ggccggcgaa acccgcgttg gactgagccc atttttgaga aatccaaccg gggcccagta
      421 cggaatcaag tgcagggaac ataggatttt ttaaaaacac taaattgcgc aacaacactg
      481 gacccattcc gcaaagaaac cctacggcag ctcggtgcca atgccgtggc ggcaaaacaa
      541 tcggcaacgc aatcaatgtc caaaaggcaa acactttggt ggcgtaggca aatccaagga
      601 aaaggcctac caaaaaccac gattttgaaa gtagcgcagc aaaagtcgct agagtgaaca
      661 atgctaggaa atagtcggtt ttcgccaccg cgcttgacca ttcaaaaaaa taaggcacag
      721 aagcgagcca ggcaaaaaac aaatcacgag tgttaactga gcttcctcta atttgtgcaa
      781 ttacgcgggc gccaagcaaa accactagag cgtgccccaa ccagaggtga atttgttgcg
      841 cagtgaggag cccacttata tcgtgaaaca gcgaatgtgt agcagcgatt cgcccgaagg
      901 ttgcgtgata tattccgtac agtggctcaa taaacgttgc ctgcgcaaag ctcggatgag
      961 ccgactcgag acgaatagct cccaacgaag cccaaaactt cgcggcatac acgtggtagt
     1021 aaagggggtc gctgtgctgg tgaggaatac tgaccgaaac aaatcgtgct aaaaaataaa
     1081 gccccggaaa cacgagccac cacacggggc ctttaatttt gaaattaaaa cgactgtggc
     1141 gtatcccgaa aatcgaaaat aacagaaaag gaattagtac ccaactacga taaagtccga
     1201 gtagccccaa aattaagacg ttctggcaga acacaccgag cgcgagcact aaattcactt
     1261 cagttccgac gtcatcgtac gacgacgttg aaccgccaag cttgcttgaa aaacgcccaa
     1321 tccatccgcc cgttcccaat acgctcagca cgatgagcat tgtgaaaact aacgacgtaa
     1381 taatattcca aatcaacaca cggtgctcct agttgcgcat tagagagctc tcacactcgg
     1441 tgatagctga aagttccgca aacggctgga cctgcgtaaa atcccacgcc tcatcaaatg
     1501 agtaaatggc atatcctttg tgcaatgatt tcattttttc aaaaaatgaa ctcgctggtt
     1561 cgaccaaaac ttggcctgct gaagtcgaag cgtgtatgat ggtcgtattt tgttctttgt
     1621 cacctgaccg cattatcatg gcatgcacca atcccatggg gcctgagtct ttaagatatt
     1681 tttcggcgta ggcattggtc ggtacagcaa agaccgcgaa atactggcct cgatgtttcc
     1741 caaaacattt ttctagttgt ttgggagtcc aatattcgat ctc
//
LOCUS       DAOMXO010000528         1779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_39196_length_1779_cov_5.364849, whole genome shotgun sequence.
ACCESSION   DAOMXO010000528 DAOMXO010000000
VERSION     DAOMXO010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39196_length_1779_cov_5.364849"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLH57_09950"
     CDS             <1..465
                     /locus_tag="PLH57_09950"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HRK02975.1"
                     /translation="AGYSARGTTGIDFFAPLTTKRGAAEFLRISDAVAADANVIATGL
                     LPDSPGDNRLIQQISNLQHAKTMGGGVATFDDFYNATVTELATASHRNKHVLEHQTNI
                     VTQLEKVRESISGVSLDEETTNLVQYQHAFDAAAKVIKVADEILETVLNMRA"
     gene            472..1470
                     /gene="flgL"
                     /locus_tag="PLH57_09955"
     CDS             472..1470
                     /gene="flgL"
                     /locus_tag="PLH57_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HRK02976.1"
                     /translation="MRVSDNTSAGSVKDALRRTRGRLERLQIQNATQKKLVTPSDDPA
                     GNSKILDIRTQATVNSQFANNGAMAKNRLQFADTALNELYDIFVRAKEIAINQSSGAS
                     ASVDSRLGVAQEVTALYQQLLSIANRRVGEQYLFGGFKTLSAPYTPTGEYRGDNGEMP
                     IEIQKGVYVPINVPGPVAFQIQRYKSEDQARSPAAVDKTQNSAAAPGEPPPSEQQGVP
                     SIETIDAFKEIDALRVGLLTNDTITIRDTMERLDELIKNTITVRAKVSARSNSIDQAI
                     GASEKADLSNAELATQIEDADYTELWSNLAKEETILRSSLSAAQKLIQPTLLDFLK"
     gene            1564..>1779
                     /gene="csrA"
                     /locus_tag="PLH57_09960"
     CDS             1564..>1779
                     /gene="csrA"
                     /locus_tag="PLH57_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HRK02977.1"
                     /translation="MLVLTRKLGESIAIDDHIKIVVVQIKGKQVRLGIKAPKETKIHR
                     EEVYLAIQEQNKEAVSATPDTMKDMSKA"
BASE COUNT          531 a          393 c          442 g          413 t
ORIGIN      
        1 gcgggatact ctgcccgagg aacgacaggg attgattttt ttgcaccgct cacaacaaaa
       61 agaggcgctg ctgaatttct gagaattagc gatgctgtcg cggcggatgc taatgtaata
      121 gctactggcc tgttaccgga ttctccaggt gacaatcgct tgattcaaca aatctcgaac
      181 ctacaacacg caaaaaccat ggggggcggg gtagctactt tcgacgattt ttataatgcc
      241 acagttactg agcttgcgac agcgagccat cgcaataagc atgttttaga acatcaaacc
      301 aacatagtta ctcaattgga aaaagtgcgg gaaagcattt cgggcgtaag cctcgatgag
      361 gaaactacaa atcttgtgca gtatcagcac gcattcgatg cggcggcgaa agtaattaaa
      421 gtggcagacg agattctcga aaccgttctg aacatgaggg cgtagtaatt tatgcgggta
      481 agtgacaaca catcagcagg tagcgttaaa gatgcgttgc gccgaacgcg cggtcgttta
      541 gagcgtttgc aaattcagaa tgcgactcaa aaaaaattag taacgccaag tgacgatccg
      601 gcgggtaaca gtaaaatact ggatattcgc acccaggcta ccgtgaattc gcagtttgca
      661 aataatggcg cgatggccaa aaatcgactt cagtttgcgg acactgccct aaacgaactt
      721 tacgatattt ttgtgagagc caaagagata gccattaatc aatcgagcgg cgccagcgct
      781 tcggttgact cgcgcttggg tgttgcgcaa gaggtgaccg ctctttatca gcagctactt
      841 tcaatcgcta atcgccgggt aggtgaacaa tatttgttcg gtggattcaa aaccctttcc
      901 gccccctaca caccgacagg tgaataccgt ggtgacaatg gcgaaatgcc gatagaaatt
      961 caaaagggcg tttatgttcc gataaatgta ccaggaccgg tagcgtttca aatccaaaga
     1021 tataaatccg aggaccaagc ccgatcgcct gcggcagtag ataaaactca gaattcggcc
     1081 gcagctccgg gagagccccc gcctagtgaa caacagggtg ttcccagcat tgagaccatt
     1141 gacgctttta aagagatcga cgcgctgcgc gtagggttat tgaccaacga tacaatcact
     1201 atacgcgata cgatggagcg cctcgacgaa cttattaaaa atacaattac ggtacgtgca
     1261 aaggtaagcg cgcgttccaa ctcaattgat caggcaattg gggcttcgga aaaggctgat
     1321 ctttctaacg ctgagttggc cactcaaata gaagatgccg attatacgga gctttggtcc
     1381 aacctagcca aagaggaaac cattttaaga tcgtctttga gcgctgcaca aaagcttatc
     1441 caaccgactt tgctggattt tttgaagtag gattttatta atgtgttgcg taacccccca
     1501 tctatcgata atacggaaac cctgtaaagg actactattg caaagcatta gggagtggaa
     1561 gttatgttgg ttctaacaag gaagctcgga gaaagtattg cgatcgacga ccatattaag
     1621 attgtggttg tgcagatcaa aggcaaacaa gttcgtttag ggatcaaagc ccctaaggaa
     1681 accaagattc atcgtgaaga ggtgtacctt gctattcagg aacaaaacaa ggaagctgta
     1741 agtgcgactc cggacacgat gaaggacatg tccaaagca
//
LOCUS       DAOMXO010000529         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_39260_length_1777_cov_5.419280, whole genome shotgun sequence.
ACCESSION   DAOMXO010000529 DAOMXO010000000
VERSION     DAOMXO010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39260_length_1777_cov_5.419280"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLH57_09965"
     CDS             complement(<1..191)
                     /locus_tag="PLH57_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRK02978.1"
                     /translation="MNLLQIEVQLANKVQEITSLHVDPAHDYLHFKRVVATSKFLCTQ
                     EQARLEVVLPAAWLHDLVIV"
     gene            241..1479
                     /locus_tag="PLH57_09970"
     CDS             241..1479
                     /locus_tag="PLH57_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRK02979.1"
                     /translation="MTTNIISEFLHTLSLPEVKYTGLIFALFVFPRFLIRFGIPYALT
                     AFAMGVCASFLEWELGGSDAVKLLSSLGIISLFLFAGLEVEFGELRQSRRFLVQHVIV
                     SVVLLIISSVGLTYWLKLDVRPAVLLGLALLTPSTGFILDTINTSSVPEAGKFWIRSK
                     AIAAEIVALFLMFVLVRSMSAQEFTFAMVGMGLLILALPLAFKFFAKVVEPAAPKSEF
                     GFLLMIALLSGMITKKLGAYYLIGAFVVGIVARRFEGSMPRLTSKPMLRSIRSFTGFF
                     IPFYFFHAGSSVSKEELSLKSLGIGLAMIAVFLPLRIYSVVWHRKLALREAIHQSFPV
                     AASLLPNLVFGLVLAELLKTTFQVPLDIVGAVIIYTVGATLAPPILMRVFWKSPEYAF
                     LAEAESNLGATWGDEDANPR"
     gene            1540..>1777
                     /locus_tag="PLH57_09975"
     CDS             1540..>1777
                     /locus_tag="PLH57_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02980.1"
                     /translation="MANRETDPTWRKRSNPEPWEFAGWGTAAKLELGDESVPIQTLGV
                     SSKQSDHLGELASTAICGNDITSSVLYVSALTIGV"
BASE COUNT          384 a          393 c          468 g          532 t
ORIGIN      
        1 acgatgacca ggtcgtggag ccaggcggca ggaagcacga cctccaggcg agcttgttcc
       61 tgggtacaca aaaacttcga ggtggcgacc acgcgtttaa agtgtaaata gtcgtgggcc
      121 gggtccacat gcagcgacgt aatttcttgg actttatttg ccagctgtac ttcaatttgg
      181 agtagattca ttattatctg gcatacctca gatattgcgt tttgaagaga tcatccgatt
      241 atgacaacga atattatttc agagtttcta cacacgctct cactacccga ggttaagtac
      301 acggggctta tatttgcgct cttcgtgttt ccgcgatttt taattcgttt cggaattcca
      361 tatgcgctta cagcgtttgc catgggtgtg tgtgcttctt ttttggaatg ggagcttggc
      421 gggagcgacg cggtaaagct cctttcgtct ttgggaatca tttcgttgtt tctgtttgca
      481 gggttagaag tcgagtttgg cgagcttcgt cagagtcgtc gctttttagt gcaacacgta
      541 attgttagtg tggtgcttct gattatttct agtgtgggac tcacttattg gttgaagtta
      601 gatgttcggc ctgcggtttt gttggggctc gcgttgctca cgccatcgac cggatttatt
      661 ttagatacca tcaacacttc gagcgtgccg gaggccggaa aattttggat tcgctccaaa
      721 gcgattgcgg cggaaatcgt agcactattt ttaatgtttg ttttggtgcg ttccatgagt
      781 gcccaggaat ttacgtttgc catggtggga atgggcttgc ttattttggc gcttcccctg
      841 gcgtttaagt tttttgcaaa agttgtggag ccggctgccc ctaagtccga attcggtttt
      901 ctactcatga tcgcgctgct ctcgggcatg atcacaaaaa aactaggtgc ttattacctg
      961 attggggctt tcgtcgtagg aattgtcgcg cggcgcttcg aaggcagtat gccgcgacta
     1021 acgtcgaagc cgatgttgcg atctattcgg tcttttaccg gtttttttat tccgttttat
     1081 tttttccatg ccggatcgtc ggtttcaaaa gaagaacttt cactgaaatc tttgggaatc
     1141 gggctcgcaa tgattgccgt gtttctgcca ctgcgcattt attcagtcgt atggcatcgc
     1201 aaactcgctc tgcgcgaagc tattcatcaa agttttcccg tcgcggcctc gctattgccc
     1261 aatttagttt tcggtttggt gttggccgag ttattaaaaa ccacatttca agtaccgctt
     1321 gatatcgtag gagcggtgat catttacacg gtgggagcaa ctcttgcgcc gccaatcttg
     1381 atgagggtgt tttggaaatc tccagaatac gcatttttgg ccgaagccga aagcaatctg
     1441 ggcgctactt ggggcgacga agatgccaac ccacggtgat agtgacctaa cgaaagtttg
     1501 actttggaat ccactggttc ggcgttaagc tagagcgaga tggcaaatcg cgaaacggac
     1561 ccaacttggc ggaaacgatc gaacccggaa ccttgggaat ttgccgggtg gggcactgct
     1621 gcaaaattag aattaggcga cgagtccgtt cccatacaaa cgttaggcgt tagcagcaaa
     1681 caatccgacc atctcggaga actcgcatca accgcgattt gcggtaacga cattacttca
     1741 agtgtgcttt atgtgtcggc gctcaccatt ggagtgg
//
LOCUS       DAOMXO010000530         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_39335_length_1775_cov_4.391279, whole genome shotgun sequence.
ACCESSION   DAOMXO010000530 DAOMXO010000000
VERSION     DAOMXO010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39335_length_1775_cov_4.391279"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..603)
                     /gene="rpsI"
                     /locus_tag="PLH57_09980"
     CDS             complement(211..603)
                     /gene="rpsI"
                     /locus_tag="PLH57_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRK02981.1"
                     /translation="MAEKVYHAVGKRKTAVARVYMQAGEGKIVVNERGFEDYFSRPTS
                     RMVVMQPLELTAQTGKWDIEVNVYGSGAAAQAAAAKHGISKALLEIDGALRGTLKKAG
                     FLTRDDRMVERKKYGMSGARKRYQYSKR"
     gene            complement(621..1082)
                     /gene="rplM"
                     /locus_tag="PLH57_09985"
     CDS             complement(621..1082)
                     /gene="rplM"
                     /locus_tag="PLH57_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRK02982.1"
                     /translation="MRKTFQEKNINQNKKAWYLFDANNQIVGRLATQIARILVGKHKA
                     TYTRHVDTGDFVVVVNADKVSLTGQKWSDKLYYDHTGFVSGLKTKTAKEMLDKHPEEI
                     LRRAVWGMMHKSKQAKDQLTKLKIYTGTDHPHAAQKPTEWKAPARKSKAKN"
     gene            1351..>1775
                     /locus_tag="PLH57_09990"
     CDS             1351..>1775
                     /locus_tag="PLH57_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRK02983.1"
                     /translation="MAVLGIPFDGAATYRAGARLGPRGIRNASVLNRNVHPLYNVHIY
                     EKLSAIDYGDVNVNPINIQATFSEVEKEYDKLLKAGVLPIAMGGDHSVLLPILRSMKK
                     KYRHFGIVHFDAHTDTADQAWGEKYHHGTPIRRAIMIFFT"
BASE COUNT          451 a          436 c          400 g          488 t
ORIGIN      
        1 aatgggtcgc tgcgcatcta ctattcgttc tgacagcttg cgcagcttct ctttataggt
       61 agtccaaacc atggccccct tattttagca tcgcttcagg cccactgggg gtataggcaa
      121 gattttctcg gtttaatgat gaaaacaaaa aagcccacgg tccgaagacc gtgggccctt
      181 tgataaccaa taataccgag tgactcgaga ttaacgtttc gagtactggt aacgcttacg
      241 agcgccggac atgccgtatt tcttacgctc aaccattcga tcatcgcgag tcaagaaacc
      301 agcttttttc aaggttccgc gaagcgcgcc gtcgatttca agcaacgctt tcgaaatacc
      361 gtgttttgca gcagcagctt gtgcagcagc accgctaccg taaacattca cttcgatgtc
      421 ccatttgcct gtttgagcag tgagctcaag tggttgcata acaaccatgc gggaagttgg
      481 gcggctgaaa taatcttcaa agccacgttc gttaacgaca attttacctt caccagcttg
      541 catgtaaacg cgggctactg ccgttttgcg cttaccaact gcgtgataaa ctttctctgc
      601 catttgagtt tcctaccttg ttagttctta gctttggatt tgcgggctgg agctttccac
      661 tcagttggct tttgagccgc gtgtgggtga tccgtgccag tgtaaatctt taacttggtg
      721 agttgatctt tagcttgttt gctcttgtgc atcatgcccc atactgcacg acgaagaatc
      781 tcttcggggt gcttatcgag catttctttg gcagtcttag tttttaggcc gctcacgaaa
      841 cccgtgtggt cataataaag cttgtcgctc catttttgac ccgtcaaaga aactttgtct
      901 gcgtttacaa ccacgacgaa atcgcccgta tcaacgtgac gagtgtaggt ggccttgtgc
      961 ttcccaacga gaattctcgc tatctgggta gctaggcgcc caacgatctg gttgttggca
     1021 tcaaagagat accaagcctt cttgttttga ttgatgtttt tttcttggaa tgttttacgc
     1081 atagaggcga atggttatcg cacagggttg gttgaagtca acgtgaaatt acaatacgat
     1141 gttcgcaaat ccaaacccat acccgctttc gtgaggtgac ccatggcgcg acctaaaacc
     1201 acaaagaaga ccccaaaaaa taaacaaaat cggtcaacta cagtgacctt gaagggacat
     1261 gcccctaaaa atccactact tagccctcga tttggtgaca tcgcgacgtt tggacgactg
     1321 ccccataccc ggaatttgaa gggggtaaac gtggccgtgt tgggtatccc gttcgacgga
     1381 gctgccacct accgcgccgg tgctcgtcta ggacctcgcg gtattcgtaa tgcttcagta
     1441 cttaatcgaa acgtgcaccc gctatataat gtgcacattt acgaaaaatt aagcgcaatc
     1501 gattacggag acgtaaacgt aaacccaata aatattcagg ccacgttttc agaagtagaa
     1561 aaagaatacg acaaactact caaagcgggc gttttaccaa tcgccatggg tggagaccat
     1621 tcggtattac ttcctatttt acgatcaatg aaaaaaaagt atcgccactt cggcattgtt
     1681 catttcgacg ctcacacaga cactgcagat caagcgtggg gtgaaaaata tcatcatggc
     1741 actcctattc gccgtgccat tatgatattt ttcac
//
LOCUS       DAOMXO010000531         1774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_39380_length_1774_cov_3.870273, whole genome shotgun sequence.
ACCESSION   DAOMXO010000531 DAOMXO010000000
VERSION     DAOMXO010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39380_length_1774_cov_3.870273"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PLH57_09995"
     CDS             complement(<1..1108)
                     /locus_tag="PLH57_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02984.1"
                     /translation="MKKNLLQTLGLFAAFITFSAQGCGTSINDRISELNDLFEREVAE
                     FDDFQHLVQDSTASSNANWDELGISAIVWSETVQLINKKLGLLVDKLYLLDSDDRYSY
                     KPALLGMIDKYDAFLNRNADLVVVAEPWNAPVLSHALDLALKAIEHHTTPNEVRDSAA
                     EHYGALYGGTNPSTDWRIETTLDPNSDNLNRRITDSWEAEYNLICRGTGLKQTCHLTS
                     GALVGLKSHYGYFLQYFDFNSGVDVGLTTPDARVVRFETMSGDGHIKYGEPLSLKIEG
                     RGYLQRLDVAGSLPDFILTNSRIIDWEIHPIWEFMNGTLNNDGTRKLNGYEVDGRFTF
                     ALWNRTERAYLVHCPRENPQGLSWFRGNVDSLDTD"
     gene            complement(1177..1686)
                     /locus_tag="PLH57_10000"
     CDS             complement(1177..1686)
                     /locus_tag="PLH57_10000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK02985.1"
                     /translation="MRVRKAVENELNEVFLMGYDAWGASLPIDMYLDSCAASYKYKQG
                     EFYILENEAGKLLSSCIVYPLMKFGGIVSECAVGIGSLVTAPEDRHKGYATALLAALM
                     GTLEERGIDAFFIHSDIPTKIYEKLGFYAAPEDMRDRGRGVTPMLRLRGGKEVSHELW
                     REIVLPAYF"
BASE COUNT          468 a          430 c          431 g          445 t
ORIGIN      
        1 cgtcggtgtc taagctgtcg acgttaccgc gaaaccaact taaaccctgg gggttttccc
       61 gaggacagtg caccagatag gccctttcgg tacggttcca aagagcgaag gtaaagcgac
      121 catcaacttc atacccatta agtttgcggg tcccgtcgtt gttaagtgtt ccattcataa
      181 attcccagat cgggtgaatt tcccaatcga taatacgaga attcgtaaga atgaagtccg
      241 gcaaggaacc cgcaacatcc aaacgctgca aatagccgcg accttcaatt ttgagtgaaa
      301 ggggttcgcc atacttaatg tgcccgtcgc cgctcatcgt ttcaaaacga accacccgcg
      361 cgtctggggt ggtcaagcct acgtctactc cggagttaaa atcaaaatac tgcagaaagt
      421 atccgtaatg ggatttcaac ccgacaagag ccccactagt aaggtggcaa gtttgtttca
      481 gcccggtccc gcggcatatt aagttgtatt ctgcttccca ggaatcagtg attcttcgat
      541 taagattatc tgagttgggg tccaaagttg tctcgattcg ccaatcggta ctagggttcg
      601 ttccaccgta gagtgcgcca tagtgttctg cggcagaatc tcgaacttca ttgggtgtcg
      661 tatggtgctc aatagctttt agggcgaggt cgagggcgtg actaagcacg ggggcattcc
      721 aaggctcagc tactactacg aggtcggcgt tgcgattgag aaaagcgtca tatttgtcga
      781 tcatcccgag gagcgctggt ttgtaggaat agcgatcgtc ggaatctaag agatagagtt
      841 tgtctacaag tagacccagc tttttattga ttaattggac cgtttcgctc cagacaatgg
      901 ccgagattcc aagttcgtcc cagttagcgt tcgaagacgc agtgctgtct tgaaccaggt
      961 gttggaagtc gtcgaattcg gcaacttcac gctcaaaaag atcatttaat tccgagattc
     1021 ggtcgttgat gctcgtgccg caaccttgag cagaaaacgt gatgaaagcg gcaaaaagtc
     1081 ctagtgtctg cagtagattt tttttcatta aagccctcga tagccgagag tgaaaaattt
     1141 gaatcagaag gtcaaacgat aaagtcgcta tcgaagttaa aaataggcag gtaacactat
     1201 ttcacgccaa agttcgtggc tcacttcttt gcccccgcgc agtcgcagca taggggtcac
     1261 cccgcgtccg cggtcgcgca tgtcttcagg ggcggcgtag aatcctagct tttcgtagat
     1321 cttcgtagga atgtctgagt gaataaaaaa ggcatcgatt ccacgttctt cgagggtgcc
     1381 catcaaggcg gctaaaagag ccgtcgcgta acctttgtgg cggtcttctg gagcagtcac
     1441 gagacttccg atgccaacgg cacactctga aactataccg ccgaatttca ttagcggata
     1501 aacaatacaa gacgacagca actttcctgc ttcattttct agaatgtaaa actcgccctg
     1561 cttatattta taagaggcgg cgcagctatc taggtacata tcaatgggta aactggcgcc
     1621 ccaggcatcg taacccatta aaaaaacttc attcaattca ttttcaaccg ctttgcgaac
     1681 gcgcatggtg tgcccgccta tcacctagag tgtaacgcca tataacaatt tgataaagaa
     1741 ctatgacgcc ggccatcacg gcccagaacg ctaa
//
LOCUS       DAOMXO010000532         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_39559_length_1767_cov_5.327687, whole genome shotgun sequence.
ACCESSION   DAOMXO010000532 DAOMXO010000000
VERSION     DAOMXO010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39559_length_1767_cov_5.327687"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLH57_10005"
     CDS             complement(<1..697)
                     /locus_tag="PLH57_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRK02986.1"
                     /translation="MVQWWQWLGGDRSTPTTQIWLAGLVSVAFAAFAIRASLTGRGAS
                     ARIQKQGSSFFLGHLPMEFGYWILGPIGKLAAALRLSPNLFSWSCLILGAASGLAAAL
                     GSIGLAGGLSILSALSDSLDGMVARTRGVASDAGEVFDAAVDRYTEFFLLAGLCVYYR
                     FEPSAMALVLLALLGSFMVSYSQAKAEAMQMEIPKGWMRRPERAAYLGGGAFLSPLLT
                     NLIEKHHPIPMHYL"
     gene            complement(734..>1767)
                     /locus_tag="PLH57_10010"
     CDS             complement(734..>1767)
                     /locus_tag="PLH57_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HRK02987.1"
                     /translation="ALLEKVRPALEKIQPMAAVFDPEFVKRINGPNVKKGKSKMDLAD
                     QLREDIRRFSRENNCSRLVVLWCASTETYIPLGDVHSTLDKFEAGLRTNDPGIAPSMI
                     YTYAALMEGVPYANGSPNQSVELPALQELSRIKGVPIAGSDFKTGQTLIKTVLAPGLK
                     ARLLGLSGWYSTNILGNRDGEVLDDPGSFKSKEITKLSVLDQILQPEQYPELYGDVHH
                     VVRINYYPPRGDNKEGWDNIDIFGWLGYPMQIKVNFLCRDSILAAPVALDIALFIDLA
                     SRAGFSGVQEWMSFYFKSPMVAPGLKPEHDLFVQQLKLQNTLRFLAGEELINHLGLTY
                     YGVDESDAR"
BASE COUNT          452 a          455 c          465 g          395 t
ORIGIN      
        1 ataggtagtg catggggatc gggtgatgtt tttcgatcag gttcgttaga agcgggctca
       61 aaaatgcacc accacccaga taggctgcgc gctcaggtcg gcgcatccag cctttcggaa
      121 tttccatttg catggcctcg gctttggctt gggaataact caccataaat gaaccaagta
      181 aagcgagtag caccagcgcc atggcggacg gctcgaaacg ataataaacg cagagcccag
      241 ccagtaaaaa aaactcggtg tagcgatcta ctgccgcgtc gaaaacctcg cctgcatctg
      301 aggcgacgcc gcgggttcgg gcaaccattc cgtcgaggga atccgaaagg gcagacaaga
      361 tgctcaggcc accggccaga ccaatcgaac cgagggctgc agctaagccg ctggctgctc
      421 ccaaaattaa acaagaccat gaaaaaagat tcggcgacag tctcagcgcg gcggcgagtt
      481 taccgatggg gcctaatatc cagtagccaa actccatggg cagatgccca agaaagaaac
      541 tagatccctg cttttgaatg cgagcgctgg ctccacggcc cgttaacgag gcgcgaatgg
      601 caaatgctgc aaatgccacg ctcacgagac cggcaagcca gatctgcgtg gtcggagtag
      661 agcgatctcc ccccagccat tgccaccatt gcaccacgaa aaaaaacctc ccgtacgagt
      721 aaccgcagcg cgattagcgc gcgtcgcttt catcgactcc gtagtatgtg agtcccaggt
      781 gattgatgag ctcctcacca gcaagaaatc gcaacgtatt ttgaagcttg agctgctgta
      841 cgaaaagatc gtgctctggt ttcagacctg gagcaaccat ggggcttttg aaataaaagc
      901 tcatccattc ctgtacgccc gaaaaacctg cgcgagaagc gaggtcgata aaaagcgcaa
      961 tgtccagtgc cacgggtgcg gccaaaatac tatcgcgaca gagaaagttc actttgattt
     1021 gcatggggta accgagccaa ccaaaaatat cgatgttgtc ccaaccctct ttgttgtcgc
     1081 cccgaggagg gtagtagtta atacgtacta cgtgatgaac gtcgccgtag agttctgggt
     1141 actgttcagg ttgcagaatt tgatcgagca ccgaaagctt ggtgatctct tttgatttga
     1201 acgaacctgg atcgtcgaga acttctccat cccggttgcc caggatgttc gtcgagtacc
     1261 agcctgaaag gccaaggagt cgcgctttta ggcctggagc caaaaccgtt ttgataagcg
     1321 tttgcccagt tttaaagtcg cttccggcaa ttggtacgcc tttaattcgg gaaagttcct
     1381 gaagagcagg caattcgacc gattggtttg gcgaaccgtt ggcgtagggc accccttcca
     1441 tcaacgctgc gtaggtatag atcatcgacg gtgcaatgcc cgggtcgttt gttctcaagc
     1501 cggcttcgaa tttgtcgagt gtcgaatgca cgtcgccgag tggaatgtag gtttcagtgg
     1561 aagcacacca aagtacaact aatcgggaac agttgttctc tcgactaaac cggcgaatgt
     1621 cttcacgcag ttgatcggca agatccattt tggacttacc ttttttaaca ttgggtccgt
     1681 tgatccgctt aacaaactca ggatcaaaaa ccgctgccat gggttgaatt ttttctaatg
     1741 ccggacgcac tttttcgagt agcgctg
//
LOCUS       DAOMXO010000533         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_39579_length_1767_cov_3.115070, whole genome shotgun sequence.
ACCESSION   DAOMXO010000533 DAOMXO010000000
VERSION     DAOMXO010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39579_length_1767_cov_3.115070"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..641
                     /locus_tag="PLH57_10015"
     CDS             255..641
                     /locus_tag="PLH57_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02988.1"
                     /translation="MKLSGKELKTLFNAIGDEKTLGALLLTFYQRMSEDLLIGYFFDG
                     KNLPHIAEQQKNFLLKAWGIKPSYSGKSPAQAHRDLPEILAGHFDRRIVILKEVLESA
                     GFSSQVVKTWTDFEKSFRKQIVKGTK"
     gene            638..1315
                     /locus_tag="PLH57_10020"
     CDS             638..1315
                     /locus_tag="PLH57_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRK02989.1"
                     /translation="MTELITWLKGLHSAEGIAAIVQAGGLVALIGIIFAETGLLVGFF
                     LPGDSLLITAGLLANPENPHYISTLNIVFLNVALCIAAIVGDQVGFYLGRKTGNAIWN
                     RPDGRFYKKKHLVAAHEFYEKWGGLSVVGARFVPILRTFVPFAAGVARMPYRNFVFWN
                     IGGGILWITSLLWIGYFLGQTPLANRLDKIIVLVIFVSILPLIFGTAKAVIKSKWGGR
                     EGTTPLV"
     gene            complement(1299..>1767)
                     /locus_tag="PLH57_10025"
     CDS             complement(1299..>1767)
                     /locus_tag="PLH57_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02990.1"
                     /translation="GFDCEFDMGSTLAVPGCAINESRSDEQILNVARGRLPLVSLVSN
                     TQNQFIEHFLRAVPDWDLLAILGPIRTFVWEIRFKTIVESESPKATLEFWMLPDGRGA
                     GEISMRVPTDQALAERERLVEYLRNLGITKIALDESKTAWAMRYFASKLKPAE"
BASE COUNT          449 a          412 c          412 g          494 t
ORIGIN      
        1 cctataccgc gatttttgcg gaccgtccca caaaaatcgc ggtataggtc tctttctttc
       61 cgattcagcg ccttgaatcc aggggcgttt gaggcatttt tcaagattca ctgatcgcaa
      121 gtgggcgctt aagtgcctgc accaatcctg tacggcttgc gaggcccccc gggcttcagt
      181 atgaagtaga aatacggttg caaaaataat tgcaatgaca gactttgaca cgctgtatag
      241 gcacactttc taccatgaaa ctttcaggta aagaactcaa gacccttttt aacgcaatcg
      301 gcgatgaaaa aacactcggc gcactattgc ttacatttta ccaaagaatg agcgaagatc
      361 ttctcattgg ctattttttt gatggtaaaa atttgcctca catcgcggaa cagcaaaaaa
      421 actttctttt gaaggcctgg ggtattaaac ctagttacag cggcaaatct cccgcacagg
      481 cgcatcgaga tttaccggaa attctagctg ggcacttcga ccggagaatc gtaattctta
      541 aagaagtgct cgaaagtgcg ggcttcagta gccaagtagt taagacgtgg actgattttg
      601 aaaaaagttt tcgaaaacaa attgtaaagg gcacaaaatg acggaattga taacttggct
      661 caaggggtta cactcggcgg aaggtattgc ggctatcgtc caagccggtg gcctggtggc
      721 gttgatcgga attattttcg cggaaacggg actgcttgtc ggtttttttc tacctggaga
      781 ctccctgtta atcaccgcag gcttattagc taatcctgag aaccctcact atatttccac
      841 actaaatatt gtttttttaa acgtcgctct ctgcattgct gccattgtgg gcgaccaggt
      901 cggtttttat ttaggccgaa aaactggcaa tgccatttgg aatcgcccgg acggtcgctt
      961 ttataaaaag aaacatttgg ttgcagccca cgaattttac gaaaaatggg gcgggctttc
     1021 cgtggttggc gcacgttttg ttcctatact acgaactttt gttccgtttg ccgcgggcgt
     1081 tgcgcgaatg ccttatcgaa attttgtttt ttggaacatc ggtggtggca ttttgtggat
     1141 cacctcgctg ctttggattg ggtattttct aggccaaact ccgcttgcaa accgactcga
     1201 taaaattatc gtgcttgtaa tttttgtttc gattctcccc cttatttttg gaactgcaaa
     1261 agcagtcatt aaatcaaaat ggggtggtcg cgagggtact actccgctgg tttgagcttg
     1321 ctcgcgaagt agcgcattgc ccaagccgtt ttggattcat ccaacgcgat tttagtaata
     1381 ccgagatttc gaagatactc tacgaggcgt tcccgctcgg ccagtgcctg gtcggtaggt
     1441 actctcattg aaatttcacc agctccgcgc ccgtcgggca gcatccagaa ctctaaggta
     1501 gccttgggag attccgactc cacgattgtt ttaaatcgaa tttcccaaac gaacgtgcga
     1561 atgggcccca aaattgcgag caagtcccag tccggcactg ctctgagaaa atgctcgata
     1621 aattggtttt gagtgtttga aaccagcgaa accaaaggaa gtcgacctcg cgctacattt
     1681 aaaatttgtt catcgcttcg ggactcgtta atggcgcaac ccggaacagc caacgtcgac
     1741 cccatatcga attcacaatc aaatcct
//
LOCUS       DAOMXO010000534         1764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_39657_length_1764_cov_4.637800, whole genome shotgun sequence.
ACCESSION   DAOMXO010000534 DAOMXO010000000
VERSION     DAOMXO010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39657_length_1764_cov_4.637800"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLH57_10030"
     CDS             <1..707
                     /locus_tag="PLH57_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02991.1"
                     /translation="EAKAKADKAFVKELSKRADQLFLAELSQLDIIAEHLGSYKTAGG
                     NAIVAISKSNPDDILVIKKPYQVNEDTFSIEILEPTAIAEEIQFSNFRRDALKVIREN
                     VKESGHTPRWKVVEGHDDAAAKKLEKYGIVVRPYKRSRTVAASRTPDNIPVNKRSERA
                     KPFSEKSEAIAREFKKDIEDYEVEIISLGEARFGIAYTNELGPVSSEVGAYSFKVGID
                     QNFGNNMTEDGYVFDW"
     gene            complement(712..1362)
                     /locus_tag="PLH57_10035"
     CDS             complement(712..1362)
                     /locus_tag="PLH57_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRK02992.1"
                     /translation="MSTERIPSKARIRLAPSYWLHDFVVPFSEDIQPASLFLRNRHYL
                     VVDIETQKIAQDVGGWAHIDKLGVSVACAFDSKTGEFLSYRENELDRLQKLCRERLVV
                     GYNIIGFDLKVLSAYGFDSKKYDVFDMMLDIEQASGWRYVKLDSIAKCTLGTAKSADG
                     LQAVEWYKRGEIEKIIEYCIKDVEITRDVFVYGMKHGHIKLSKSEDKIVTVPIQWT"
     gene            complement(1359..>1764)
                     /locus_tag="PLH57_10040"
     CDS             complement(1359..>1764)
                     /locus_tag="PLH57_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02993.1"
                     /translation="VKTAWCDASNQAVLTLYRAFPFEGGRAMLYSPPAIETYKREFEE
                     QAVQQILPMLGLTSSDIAGVRIARWGHPMPVAKVGTYAKGIPDLLRKPHRESIFFAEQ
                     DNFLLPCIETCAEEAFWVAPRVRQLLKKGSSA"
BASE COUNT          495 a          437 c          394 g          438 t
ORIGIN      
        1 tcgaagcaaa ggcaaaggca gacaaagctt tcgttaagga gctttcgaaa cgagcagacc
       61 aactctttct tgctgaactt tcacaactgg atatcatcgc agaacatttg ggtagctata
      121 aaaccgccgg tggaaacgca attgtggcaa tttcgaaatc aaatcccgat gacattctgg
      181 tgatcaaaaa accttaccaa gtaaatgaag atacattttc gattgaaata ctcgaaccca
      241 cagccatcgc tgaagagatt cagttttcga actttcgtag agatgccctt aaagtaattc
      301 gcgaaaatgt taaggaatcg ggccatactc ctcgctggaa ggtagtcgaa gggcacgacg
      361 atgccgcagc gaaaaaactc gagaagtacg gaatcgtggt tcgaccgtac aaacgctcaa
      421 gaactgtagc cgccagtcga actccagata acattcccgt aaataaacgt tctgagcgag
      481 cgaaaccgtt ttcagaaaaa tcagaagcta ttgcccgcga atttaagaaa gacatagaag
      541 actacgaggt cgagattatt tctttaggtg aagcgcgatt cggcatcgca tacaccaacg
      601 aactagggcc cgtctcaagc gaggtaggcg cctactcttt caaggtcggt atcgatcaaa
      661 atttcggcaa caatatgact gaagacggct atgtgtttga ctggtgatcg cttaagtcca
      721 ctgaatcggg actgtcacaa ttttatcttc ggacttagac agtttgatgt gcccatgttt
      781 cattccgtaa acgaatacat ctcgcgtgat ctcgacgtct tttatgcagt actctataat
      841 tttttcaatc tcgccgcgtt tgtaccactc gacagcttgc agaccatcgg cgcttttggc
      901 ggtgcccaag gtgcattttg cgatcgagtc gagcttcacg taccgccagc ccgaggcttg
      961 ctcgatatcg agcatcatgt cgaagacgtc gtattttttg gaatcaaatc cgtaagccga
     1021 cagtaccttt aaatcgaaac caataatgtt ataccctacc accaaccgtt cacgacacag
     1081 cttttgcaac cgatcgagtt cattttcgcg gtaagaaaga aactctcctg ttttggaatc
     1141 gaacgcacac gccaccgaca cgccgagttt atctatgtgg gcccaacccc caacgtcttg
     1201 tgcaattttt tgagtttcaa tatcgacgac cagatagtgg cgattacgca aaaacaaact
     1261 cgcgggttgg atatcttctg agaacggaac aacaaaatcg tgcagccagt acgaaggtgc
     1321 caatcgaatt cgtgccttag acggtattct ttcagtgctc aagcgctaga tccttttttt
     1381 aaaagctgtc gtactctggg agcaacccag aacgcttctt cggcgcaggt ctcaatgcaa
     1441 ggtaacaaaa aattatcttg ttcggcaaag aagatacttt ctcgatgcgg ttttcgcaaa
     1501 agatcaggaa taccctttgc gtaggttccc accttagcca ccggcattgg gtggccccaa
     1561 cgagcaatcc gcacgccggc aatatcggaa ctcgttaacc ccaacattgg caatatttgc
     1621 tgcaccgcct gttcttcaaa ttcgcgtttg taagtttcga tggccggggg actataaagc
     1681 atggcccgcc ctccttcgaa cggaaacgca cggtacaagg ttaaaaccgc ttggttcgac
     1741 gcgtcgcacc aagcggtttt aacc
//
LOCUS       DAOMXO010000535         1763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_39695_length_1763_cov_3.436768, whole genome shotgun sequence.
ACCESSION   DAOMXO010000535 DAOMXO010000000
VERSION     DAOMXO010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39695_length_1763_cov_3.436768"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1174
                     /locus_tag="PLH57_10045"
     CDS             221..1174
                     /locus_tag="PLH57_10045"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HRK02994.1"
                     /translation="MSTKSYQKPTDEELKSRLTAEQYRCTQEAGTEKPFANAYWDHKE
                     DGIYVDVVSGEALFSSIDKYDSGSGWPSFTKPLESQNIKSIQDRSHGMDRTEVRSKNA
                     DSHLGHVFDDGPKQAGGLRFCINSAALRFVPLAQMKTQGYGAFLFAFAEKKHWQIATL
                     AGGCFWGMEELLQKLPGVIETQVGYTGGTLENAVYTEVKKGTTGHAEAVQVLFDPAFI
                     SYEKILLEFFRIHDPTTVDRQGNDIGTQYRSAIFFHNAEQKQIAERVKKRVNDSGKWK
                     KPVVTALVPQARFWRAEEYHQKYLDKTPNGYTCHFARPFEF"
     gene            complement(1182..>1763)
                     /locus_tag="PLH57_10050"
     CDS             complement(1182..>1763)
                     /locus_tag="PLH57_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02995.1"
                     /translation="LYWSGEIKVRVVPKEDRSHFYDESPYKDHYVTGATIGEREKWLK
                     SAASYIDRLLREGARKWSAEGIGLKSEKKADLVRIELQVIRGNIRKVLLGAFYLTRSG
                     GKKGPATHRLDEEQASALWIEFLAALERDLNASPLPVPEGFPMARFLMGSKHLPEAAT
                     DIRDRYVDIQKFLGPKASAEVLEIIANAISPER"
BASE COUNT          459 a          459 c          408 g          437 t
ORIGIN      
        1 taatcatggc cgacaccaaa gtagagcacg gcaaagttat ttcggttgtt gatcgcctta
       61 aatccgaagg tattcagaag ttctcttttg gagtggaaaa gcattgatga gtcgttttaa
      121 gcggtggtgc ttgctgcctg cgcttgtgct actcgtctca tttgcttgta atcgccagtc
      181 cgtgttaaac gctcctgcca aggaacccag agaaccactc atgagcacga agtcgtacca
      241 aaaaccaacc gatgaagaac taaagtcgcg gctcaccgca gaacaatacc ggtgcacgca
      301 agaggctgga actgaaaaac cattcgccaa cgcctactgg gaccataaag aagacggcat
      361 ttatgtcgac gtcgtcagcg gagaagctct atttagttcg atcgataaat atgattccgg
      421 tagcggatgg ccaagtttta ccaaaccgtt agaatcacaa aatattaagt cgattcaaga
      481 ccgttcccac ggaatggatc gtaccgaggt tcggtctaaa aatgcggact cccacttagg
      541 tcacgttttt gatgatggcc ccaaacaagc cggggggctg cgcttctgta tcaattccgc
      601 cgcgcttcga ttcgtgccac ttgctcaaat gaaaacccag gggtacgggg catttctttt
      661 tgcttttgct gagaagaaac attggcaaat cgctactctg gcgggcggct gtttttgggg
      721 catggaggaa ttacttcaaa agctcccagg agtgatcgaa acccaagtgg gttacactgg
      781 cggaacgctt gaaaatgccg tttacaccga agtcaaaaaa ggcactaccg gccacgccga
      841 agctgttcag gtgttgtttg accctgcgtt cataagttac gaaaaaatct tgttagagtt
      901 ctttcgcatc catgacccga caactgttga tcgccagggc aatgatattg gcacccaata
      961 tcgctcggcc attttctttc acaacgcgga acaaaaacaa atcgcggaac gtgttaaaaa
     1021 acgcgtgaac gactctggaa aatggaaaaa accagtcgtc accgctttgg ttcctcaagc
     1081 gcggttctgg cgagccgaag aatatcacca gaagtactta gacaaaaccc ccaacgggta
     1141 tacttgccac ttcgctagac catttgaatt ctaggccacc actagcgttc aggagaaatc
     1201 gcattggcaa taatttctaa aacttcggcc gatgccttgg gtccaagaaa tttttgaata
     1261 tctacatacc gatctctaat gtcagttgcc gcttctggga gatgcttact gcccattaag
     1321 aaacgggcca ttggaaagcc ctcgggaacc ggcagcggac tcgcattcaa atcgcgttcg
     1381 agcgccgcca aaaattctat ccacaacgcg ctcgcttgct cttcatctag tctgtgggtc
     1441 gccggtcctt tttttccgcc agacctcgtg agatagaagg ccccaagcaa taccttacga
     1501 atattccccc gaatcacctg taactcgatg cgcacgagat ccgctttttt ttccgatttt
     1561 aatccgatgc cctcagcgct ccactttcgc gctccctcac gcagcaaccg gtctatgtag
     1621 ctcgccgcgg acttgagcca tttttcccgc tctccgatag tcgctccagt gacatagtga
     1681 tccttgtaag ggctttcgtc gtagaagtgt gagcgatctt ctttggggac tactctcact
     1741 ttaatctcgc cagaccaata aag
//
LOCUS       DAOMXO010000536         1750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40090_length_1750_cov_3.541593, whole genome shotgun sequence.
ACCESSION   DAOMXO010000536 DAOMXO010000000
VERSION     DAOMXO010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40090_length_1750_cov_3.541593"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PLH57_10055"
     CDS             complement(<1..808)
                     /locus_tag="PLH57_10055"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRK02996.1"
                     /translation="MTRIKALVNVFAILAAGIAFFWAATPTLATASNCGEIVATAAPG
                     LETAIEIPVVILGAGPGGLSVAYELSKRLPMTAVLTIDQGERPGDTWRRRMPSDFPLV
                     TRWEHSALEGPFGNRFPNAEQVSAKDFADYLERYAHSVVPRVRTNTAVYAVSKQLDGR
                     LRLETSTGVIIAKQVVNATGNFSKPELPQLPGRLESAIPQMHSSEYLGEEQLRALGIP
                     IGAKQPILVVGNGTSAAQLLYALSKSGYNVALSLRTGVAGAEQSSKDLLLS"
     gene            complement(850..>1750)
                     /locus_tag="PLH57_10060"
     CDS             complement(850..>1750)
                     /locus_tag="PLH57_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK02997.1"
                     /translation="NGPTVRDYVDTLTRELVGRTEFRQTLDLGTASELENFLNQKFLW
                     KHDSQDQEFHGEEELFQAEIQADKVVGDLAILTRWLELSVRDEITTTEIERRKVFGRM
                     IDLASELLAIATIPHEVVARTKGAAVALRPDGFKIKYTNYTNRFYDPVAAFENLKRVE
                     ADRSDIVVGIASELAHSPVERGKEEVRRIVVGARDRAFAEMAEFVALHRRDFYWRANA
                     ENFDRLTIEETKQAIIGSTRVVVLAAPAPYDRTVGRLLIPANHSWVRFEHPDFGYRPP
                     ESLMVGEYLIPLAEPNKTQRVIR"
BASE COUNT          419 a          498 c          410 g          423 t
ORIGIN      
        1 tcgataacaa caaatcctta ctcgactgtt ccgcacccgc cacgcccgtt cggagcgaaa
       61 gcgctacgtt gtagccactt tttgaaagcg catacaacaa ctgcgcggcc gaagtgccgt
      121 tacccacgac taaaatgggt tgtttcgccc ctatcggaat tcccaatgcc cgcaattgtt
      181 cctcgcccaa atactccgaa gaatgcattt gcggaatagc cgactctaaa cggccaggta
      241 gttgcggaag ctcaggtttc gaaaagttcc ccgtcgcatt caccacttgt ttggcaataa
      301 tcactccggt cgatgtctcg agccgtagcc gcccgtcgag ttgcttactc accgcgtaca
      361 cggccgtgtt agtgcgaacg cgcggcacca ccgagtgagc ataacgttct agataatccg
      421 caaaatcttt ggccgaaact tgttctgcat tcggaaaacg atttccaaac gggccctcaa
      481 gcgcgctgtg ttcccaacgc gtgacgagcg gaaaatcaga tggcattcgg cggcgccaag
      541 tgtcgccagg gcgctcgccc tggtctatcg taagcaccgc agtcatcggc aagcgttttg
      601 aaagttcgta cgccacgctc aaaccgccgg gccctgcgcc caagattacc accggtattt
      661 caatcgcagt ctccaaaccc ggtgccgctg tcgcaactat ttccccgcag ttcgatgcag
      721 tcgccaacgt gggcgttgct gcccaaaaaa atgcaattcc agcagctagt attgcgaaaa
      781 cgttaactaa cgctttgatt ctagtcatac agtctaagtg tcctctatgc cgggggtttt
      841 actgtgaagt taacgaataa ctctttgcgt tttgttcggc tctgcgaggg gaattaagta
      901 ctcgccaacc atcaaactct caggtgggcg atacccaaaa tctgggtgtt caaaccgcac
      961 ccaagaatgg tttgcaggaa ttaaaagtcg cccaactgtg cggtcgtaag gtgccggcgc
     1021 cgccaacacg accactcgag tcgaaccaat aatggcttgc ttagtttctt caatcgtcaa
     1081 gcggtcaaag ttttcagcat ttgcacgcca gtaaaaatcg cgtcggtgaa gcgccacgaa
     1141 ttcagccatc tccgcaaacg ctcggtcgcg cgcgccgaca actattcggc gtacttcttc
     1201 tttgccacgt tcaaccggcg agtgcgccaa ttcgctagca attccaacca cgatgtcgct
     1261 acggtctgct tcgacacgct ttaaattttc gaaggcggcc acagggtcat aaaagcggtt
     1321 ggtataattg gtgtacttaa ttttaaatcc gtcagggcgc aacgcgacgg cagccccctt
     1381 cgtgcgtgcg acaacctcat ggggaatcgt tgcaatggcc agaagctcgc tcgctaagtc
     1441 gatcatacgg ccaaacactt tgcgtcgttc aatttcggtt gtggtaattt cgtcacgaac
     1501 tgaaagttca agccaacgcg ttagtatcgc cagatcaccc acaactttgt ccgcttgaat
     1561 ctcggcttga aaaagttctt cttcaccatg aaactcttgg tcctggctat cgtgtttcca
     1621 taaaaatttt tgattcaaaa agttttcaag ctctgaggcc gttccaaggt ctaacgtttg
     1681 tcgaaactcc gtacgaccca caagctccct agtgagcgtg tcgacgtaat ctctcaccgt
     1741 aggtccattg
//
LOCUS       DAOMXO010000537         1748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40160_length_1748_cov_3.255759, whole genome shotgun sequence.
ACCESSION   DAOMXO010000537 DAOMXO010000000
VERSION     DAOMXO010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40160_length_1748_cov_3.255759"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="PLH57_10065"
     CDS             <1..1395
                     /locus_tag="PLH57_10065"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRK02998.1"
                     /translation="KIPKELDVIVMKCLAKNRDQRFSSADEMQRALHKFLYQYAADFN
                     PTDLADYARDLFKNDIVEDRKRLLRLNDKVERLLQSYEQIQVKEQEEATNTSTTRKVL
                     EKTINDDPNPTEPDVKKPTEERGTGSFRVVDFKEKQSSYSVEVDKVGQRASGPIEFAQ
                     VASTQVASGTKMWKSAGNGGSKRGRLHFATFGGLVLFIGAAAFIINAYFKSFPSGTSG
                     GKATVRIEGNVPAATVMINGRQVADKLPATVGGLAAGEAEVTVFASGYRRETRKINLN
                     DGEQQSVRVQLTREDSSDIATRTGREAGLAKSTVLRVNVTPAGEAPRIKVNGQLLDIS
                     GVSAAPVGVPLEITVERPGFAVFRKKIEIPATTQDEYVLDVVLREAKFGFLSIKTTPS
                     AEALITVDGIEEKLSTPFVRKKLPVGEYQVKLVNTVLGLEKIVSVRIEEDRFSNLEER
                     FYDEPSGRMPSMSR"
     gene            complement(1392..>1748)
                     /locus_tag="PLH57_10070"
     CDS             complement(1392..>1748)
                     /locus_tag="PLH57_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRK02999.1"
                     /translation="KELTARVRARLREKSARQKEPKTLVDGEIKLVLDTRDAFHGGSM
                     VSLTQIEFDLLSLLLRKRGQVISRDEIIQLVWREDPTTAHKRTIDVHIRALRKKIPVL
                     TRMITSIYGVGYKYGG"
BASE COUNT          492 a          384 c          449 g          423 t
ORIGIN      
        1 aaaatcccaa aagagctcga cgtgatcgtc atgaagtgcc ttgctaagaa tcgcgatcaa
       61 cgcttttctt ctgccgatga aatgcaacgg gcccttcata aatttctcta ccaatatgct
      121 gctgatttta atccaaccga tctcgccgat tacgctcgcg atttgttcaa aaacgatatt
      181 gtagaagacc gcaagcgact cttgcgctta aacgataaag tcgagcggtt gctgcagtcc
      241 tacgaacaga ttcaggttaa agagcaagaa gaggcgacca acacctcgac cacgcgaaaa
      301 gtgctcgaaa aaacaattaa tgacgatccg aatccaacgg agccagatgt caaaaaacca
      361 actgaggaac gaggcactgg tagcttcaga gtggtggatt tcaaagaaaa gcagagtagt
      421 tattcggtcg aagtagataa ggttggccaa cgagctagcg gaccaatcga attcgctcag
      481 gtggcaagca ctcaggtggc aagtggtacc aaaatgtgga aatctgctgg taacggcggt
      541 tcgaaacgcg gccgtttgca ctttgcaact tttgggggcc tcgtactttt tattggagct
      601 gcggccttta tcataaacgc ttattttaaa tcctttccta gtggtacttc cggtggaaaa
      661 gctactgtac gaatagaagg gaatgtgccg gccgctactg taatgattaa cggtcgccag
      721 gtggccgata agttacccgc caccgtcggt ggactagcgg ctggtgaagc tgaagtaacg
      781 gtatttgcga gtggatatcg aagagagacc cgaaaaatta acttaaatga cggcgagcag
      841 caaagtgtgc gagttcaact aacacgcgaa gattcaagtg atattgcgac ccgaacgggc
      901 cgtgaggccg gtttggcgaa atccactgtg ttgagagtaa atgtaactcc tgcgggagaa
      961 gcccctagaa taaaagttaa cggacagctt ctcgacatta gtggtgtttc agccgctccg
     1021 gtcggagttc cgcttgaaat aaccgttgag cggcctgggt ttgcagtttt tcgcaagaag
     1081 atcgaaattc cagcaactac ccaagatgaa tatgttcttg atgttgtttt gcgagaagcg
     1141 aagttcggat ttctaagtat caaaacgacg cccagtgcgg aggccttaat tacggtcgac
     1201 ggaattgaag aaaagctgtc gacaccgttt gttcgaaaga agttaccagt aggagaatat
     1261 caggtaaaac tcgtgaacac tgttctgggg ctcgaaaaaa ttgtcagcgt acggatcgaa
     1321 gaggatcgtt tttcaaattt ggaagagcgc ttctacgatg aacctagtgg ccgaatgcct
     1381 tctatgtcgc gttaaccgcc gtacttataa cccacgccat aaatggaagt tatcattcgt
     1441 gtgagtaccg gaattttttt tcgaagcgcg cggatatgta cgtcaatcgt acgtttatga
     1501 gcagtcgtgg ggtcttcacg ccaaacaagt tgaatgattt catcgcgtga aatgacttga
     1561 ccacgcttac gcaaaagtag cgaaagcaaa tcaaattcta tctgagtaag cgaaaccata
     1621 gagccgccat gaaacgcgtc acgcgtgtcg agcacaagct tgatctcacc atcgactaat
     1681 gttttcggtt ccttttggcg ggcggacttt tcgcgtaagc gagcccggac tcgagcggtg
     1741 agctcttt
//
LOCUS       DAOMXO010000538         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40243_length_1745_cov_5.157988, whole genome shotgun sequence.
ACCESSION   DAOMXO010000538 DAOMXO010000000
VERSION     DAOMXO010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40243_length_1745_cov_5.157988"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLH57_10075"
     CDS             complement(<1..312)
                     /locus_tag="PLH57_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03000.1"
                     /translation="MKKAILGWVFLIIVSTIGANQSHSDELCADGRVLPNCDVDRELL
                     EKCAADYTVYSVPFNAKLRAGAGFGSISLNNAQKKSSATGDLSLIFLVDLTRLSLEGG
                     AE"
     gene            523..1716
                     /locus_tag="PLH57_10080"
     CDS             523..1716
                     /locus_tag="PLH57_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRK03001.1"
                     /translation="MWKWKTKFRNFERTRLTVLVAVMTLSALAGCGKEQFVKQQYSST
                     ASAIGFRQIPAKVDIVIVVDNTGSMTNAFSAVQAQLSSFVNSLASKYWDYHVARVLIS
                     NPTPINRVLVDSRFNQLELPDGSANPSTDIVPAQYAVTNPNSFTLLSAAEVGAALVTD
                     PTYTSLVNTIRTARDNDVTNFFRNDALLAIIVITNGVAKEVLVDSTNPQSDVSSVRLS
                     TWANNITRLKTDVGASDDLIRFYPIASPTYRSTGCYGGSANWGGSLFQMSNYFQGVSG
                     DVCNTSSMNTVLANIESDLDVVRESYILSYIVLADPFVEGDVQAVIKNGVGIPKSNGV
                     SDGWRYVGPVSNQCTVTSIQTSTGEQPFCANVRSGYAIELIGSARARGSDSLEIVWEA
                     PGQTN"
BASE COUNT          440 a          483 c          407 g          415 t
ORIGIN      
        1 ttcggcaccg ccctctaaac tgaggcgcgt gagatctacc aaaaaaatga gtgatagatc
       61 gccggtcgca ctacttttct tctgagcgtt atttaacgaa atgctaccaa acccggcacc
      121 cgcccgtagt ttggcattaa acggaaccga atataccgtg taatctgccg cgcacttttc
      181 gagcaactcg cgatccacat cacagttcgg caacacccgg ccatcggcgc aaagttcatc
      241 ggagtggctc tggtttgccc cgatcgtgga aacaatgatc aggaataccc agcctaatat
      301 cgcttttttc atgtttttca cccttcccgg cgtccggtgg acagaaggct cgataccaca
      361 gttcattccc agtagtcacg ctaccagctc tgtctaagtt ttagacacct gcccacccgg
      421 ctcctatctc acgaaaaacc ggcccccgcc caacccattg aatattggac tgcgattttg
      481 gcaccccggt tgcactgtaa tcccgtgagc gagtgtagaa atatgtggaa gtggaaaaca
      541 aaatttcgga actttgaacg aacgcggtta accgtattgg tcgccgttat gaccctgtcc
      601 gcgctagcgg gctgcggcaa agaacagttc gtcaaacaac aatactcttc gacggcatcg
      661 gcgattggtt ttcgccaaat ccctgccaaa gtagacatcg ttatcgtggt cgacaacacc
      721 gggtctatga ccaacgcttt cagcgccgtt caggcccaat tgagctcctt cgtcaattca
      781 ctagccagca agtattggga ctaccacgta gcccgcgttc taatttcaaa cccgactccc
      841 attaatagag ttttagtgga ttcgcggttc aatcagctcg agttgccgga cggcagcgcc
      901 aatccgagca ccgatatcgt gcctgctcaa tatgcggtga cgaatccgaa ttcctttacc
      961 ttgttatctg cggccgaagt aggagccgcg cttgttaccg acccgactta cacgagtttg
     1021 gtcaacacta tccgaacagc ccgcgacaac gatgtaacaa attttttccg aaacgacgct
     1081 ttactggcga tcatcgtgat caccaacgga gttgctaaag aagtactggt ggactctacc
     1141 aatccccaga gcgatgttag ctctgttcgt ttatcgacat gggctaacaa tataacgcgc
     1201 cttaaaacag atgtcggcgc atcagacgac ttaattcgat tttaccctat cgcctcccca
     1261 acttatcgaa gcaccggttg ttacggtgga agtgcaaact ggggcggaag cctttttcag
     1321 atgagcaatt attttcaagg ggtatccggc gacgtgtgca acacctcgtc catgaataca
     1381 gttcttgcca atatcgaaag cgacctcgac gttgtgcgcg agtcctacat tttgtcctac
     1441 atcgtgcttg cagatccatt tgttgaaggc gacgttcaag ccgttatcaa aaacggcgta
     1501 ggaattccga aatccaatgg cgtgagcgac ggttggagat acgttggtcc agtcagcaac
     1561 caatgcacgg ttacttcgat tcagaccagc actggtgaac agccattctg tgctaacgtg
     1621 cgatcgggtt acgctatcga actcatcggc agcgcgcgag cccgaggcag cgactcgtta
     1681 gagatcgttt gggaagcacc cggccaaacc aactaaagcg ctcaatctgc aggcaaaata
     1741 aaact
//
LOCUS       DAOMXO010000539         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40385_length_1741_cov_2.991696, whole genome shotgun sequence.
ACCESSION   DAOMXO010000539 DAOMXO010000000
VERSION     DAOMXO010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40385_length_1741_cov_2.991696"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLH57_10085"
     CDS             <1..232
                     /locus_tag="PLH57_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03002.1"
                     /translation="GHAGISVFANVKAFMAAARGNSKVAARMIAYEIKKASDPVFEGP
                     RGNYYKKNRSAARSLEHAVSALIESNNRKVRH"
     gene            266..>1741
                     /locus_tag="PLH57_10090"
     CDS             266..>1741
                     /locus_tag="PLH57_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRK03003.1"
                     /translation="MHVVSCLRVMSLFFVLAVSLGASSCKERKSEIELVILHTNDVHS
                     RFDAGSAGHDDNIFRLGGLARLKTLIDQQRAKHPNTLLVDGGDWSEGGIFYTVDAGRN
                     ILRLMDQIGYDAAVMGNHDFLNGPVEVARRVADAKTRVKVVAANKDLSLLSSAEQKEI
                     NKYVPDYAIFKVGDLRVAVIGVTTVATEYQSFFEPGKIKDYKAATAAVAKKIHDQKLA
                     DIQIVLSHNHTSDNINIAKTVPYIQAVISGHDHNVTPKPIEVQNAGLPVYVVEAGEWG
                     KFLGKLTFAIDVKKRSIKLKKYELIPVVEKTPEHLGIKNMIAAEMRKVISRFGYDVFN
                     DEVGNCEDEMPIQRKSPTALATILAESYRESAQTDVGVEQSDLIGTGLPKGRLTSRAL
                     FDVVPYIFSPIAGRQFPQEGRSWTVHRLTLTGTELFTLLNIAATAPTLGLPIGWIAVT
                     GARLEYAPSDQWSALKGAQILNRETASYEDLNFDGTYTLALS"
BASE COUNT          482 a          428 c          421 g          410 t
ORIGIN      
        1 aggacacgca ggaatttcgg tgtttgctaa cgtcaaggca ttcatggcgg cagctagagg
       61 caactccaaa gttgcagcgc gtatgatcgc ttacgaaatc aaaaaagcta gtgatccagt
      121 gttcgaaggt ccgcgcggca attactacaa gaaaaaccgc tccgctgctc gctcacttga
      181 gcacgcagtc agtgcattga tcgaaagtaa caatcgcaaa gttcggcatt aagccgttct
      241 agttcgctaa tcagggagag atagtatgca cgtcgtttcg tgtttacggg tgatgagttt
      301 gttttttgtc ttggcggttt ctttaggagc ctcaagctgt aaagaacgca aatcagaaat
      361 tgaactagtc attctacaca caaacgacgt gcattcccgt tttgacgccg ggtcggcagg
      421 ccacgacgat aatatctttc gattaggcgg tcttgctcgc ttaaagactc tcatcgacca
      481 acagcgcgcc aagcatccga acaccttgtt ggtcgacggc ggtgactggt cagagggggg
      541 tatattctat acggtcgatg ccggtcgcaa catacttcgc ctcatggatc aaatcggata
      601 cgatgcggcg gtgatgggca atcatgactt tctaaatggt ccagtagaag tcgcgcgtcg
      661 ggtggccgac gctaaaacgc gtgtgaaggt agtggctgct aacaaagacc tttcactttt
      721 gtcgtcagcc gaacaaaaag aaatcaataa atacgtccct gattacgcaa tctttaaggt
      781 gggcgatctg cgggtggccg tgatcggagt gacgaccgtc gccactgaat accagtcttt
      841 ttttgaacct gggaaaatca aagactacaa agctgctaca gcagccgtgg ccaaaaaaat
      901 tcatgatcaa aaattagcgg acattcagat cgtactctct cacaaccaca cgagcgacaa
      961 tattaacatt gccaaaactg ttccgtatat tcaggcagtg atcagcggcc acgatcacaa
     1021 cgtcactcca aaaccaatcg aagttcaaaa cgcgggtctg ccggtctatg tcgtcgaagc
     1081 gggcgaatgg gggaaatttt tgggcaaact aacgtttgcg atcgacgtaa aaaaacgttc
     1141 gataaaactt aaaaaatacg aacttattcc cgtagtcgaa aaaactccgg aacacctcgg
     1201 aattaaaaac atgattgccg ccgaaatgag aaaagttatt tctcgattcg gttacgacgt
     1261 tttcaacgat gaagtcggaa actgcgaaga cgagatgccg atccaacgta aatcccccac
     1321 tgctctggca accattttgg ctgaaagcta ccgagaatcc gcgcagaccg acgtgggcgt
     1381 tgaacaatcc gacttgatcg gaacggggct acccaaaggg cgcctaactt cgcgtgcttt
     1441 atttgacgtc gtaccctata tttttagtcc aattgccggt cgtcaatttc cccaagaggg
     1501 gcgttcctgg accgtacatc gtctcacact cactggtacc gaattattta ctttgttgaa
     1561 tattgctgcc acggcgccaa cacttggact tccaatcggt tggatcgcgg ttaccggtgc
     1621 acggctagag tatgcccctt ccgaccagtg gagcgccctt aaaggcgctc aaatattaaa
     1681 tcgagaaacc gcaagctacg aagatttaaa cttcgacgga acctacaccc tagcgctgag
     1741 c
//
LOCUS       DAOMXO010000540         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40436_length_1739_cov_4.972090, whole genome shotgun sequence.
ACCESSION   DAOMXO010000540 DAOMXO010000000
VERSION     DAOMXO010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40436_length_1739_cov_4.972090"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLH57_10095"
     CDS             <1..594
                     /locus_tag="PLH57_10095"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRK03004.1"
                     /translation="LMTLHTSKGLEYPLVYMVGVEEGLFPSIRNGEENDEDIEEERRL
                     CYVGMTRAKERLTMTHALCRRIYGNILYHEPARFFGEIPEELVEFHDLTGGARRYSSS
                     RFSDDIDDSGFQDDFDDRASSSRSNSTGFSRPAVQYSTGGGTSDKRVGKQVRHKDYGL
                     GIVLSIEGRAEAEKITIEFAGRVTRKFVTKFAPIEFV"
     gene            642..965
                     /gene="gatC"
                     /locus_tag="PLH57_10100"
     CDS             642..965
                     /gene="gatC"
                     /locus_tag="PLH57_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRK03005.1"
                     /translation="MDRIEEIVKKTADLARLELTSDEVAMFSKQLSGILSYIEKLKSV
                     DTTGVDPLVTPLVDENGNLLTTTYLRSDELLPESSRIAAATVVETSADHIYNNFKVPQ
                     VIAQK"
     gene            991..>1739
                     /locus_tag="PLH57_10105"
     CDS             991..>1739
                     /locus_tag="PLH57_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013660923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRK03006.1"
                     /translation="MILQLHEKLKTKKISAVELTQHYLKRVKTDSNNVYITVCDREAV
                     AAAEKADQALAQGKAGLLTGIPVGIKDIFCTAGVKTTCASKILGDYVPPYDATCVARL
                     KRNGAVLLGKLNMDEFAMGGSNENSAFGPVKNPIDPRYVPGGSSGGSAAAVAGGLAVA
                     ALGTDTGGSIRLPASYTGTVGLKPTYGRVSRNGLVAFGSSLDQAGPMTTTVSDAAIVL
                     ESISGHDPFDSTSSVLPSENFLASMVTESSR"
BASE COUNT          472 a          384 c          452 g          431 t
ORIGIN      
        1 ctaatgaccc ttcacacgag taaggggttg gaatatccgc tggtttatat ggttggagta
       61 gaggaggggt tgtttccatc gattcgaaat ggtgaagaaa acgacgagga catcgaagaa
      121 gagcgacgtc tttgttacgt aggaatgacg cgagccaagg aacgtttgac catgactcat
      181 gccttgtgcc gacgaattta cggcaatatt ttatatcacg aacccgcgag attttttggc
      241 gagattccag aagaattggt tgaatttcat gacctcactg ggggcgctcg taggtattcg
      301 agctcgagat tttccgacga tatagacgac tcaggatttc aggacgattt tgacgatcga
      361 gcctcaagtt ctcgttcgaa ctcgactggg ttcagtaggc ctgcagtcca atattccaca
      421 ggggggggca cttctgataa acgggtgggc aagcaggttc gtcataaaga ctatggattg
      481 ggaatcgttc tttcgataga agggcgtgca gaggccgaaa aaattactat cgaattcgca
      541 ggtcgtgtga cccgtaagtt tgtcactaaa tttgccccaa tcgaattcgt ctgactaaat
      601 tgccggaaaa agcccgtgca ttcggcatgt taaggtgaaa tatggatcgt attgaagaga
      661 ttgtaaaaaa aacagcagat ctagcgagac ttgaactcac atctgacgaa gtcgcgatgt
      721 tttcaaaaca gctgagtgga atactttcct atatcgaaaa actgaagtcg gttgatacca
      781 caggagttga tccgcttgtc actcccttag tagatgaaaa cggaaatctc ctgaccacta
      841 cttatctcag gtctgacgaa ctcttgcccg aaagttcacg aatcgcggcc gctacagtcg
      901 tggaaacttc ggcggaccat atatataaca attttaaagt tccccaagtg atcgctcaaa
      961 aatagtagca ccgtcgaaat tggataaatt atgattctgc agttacatga aaaattaaaa
     1021 accaaaaaaa tatcggcagt ggaactaacg cagcattact taaaacgcgt taaaaccgat
     1081 tcgaacaatg tgtatataac tgtctgcgat cgtgaagcgg ttgccgcagc cgagaaggct
     1141 gatcaggcgt tggcccaagg caaggccggg ttattgaccg gaatacctgt aggaatcaaa
     1201 gatattttct gtacggcagg cgtaaaaaca acctgtgctt ccaaaatact cggagattac
     1261 gtcccgccat atgacgccac ctgcgtggct cgtctgaagc gcaacggtgc ggtacttctt
     1321 ggcaagttaa acatggatga gtttgccatg ggcggctcca acgagaattc tgctttcggt
     1381 cctgtgaaaa atccgatcga cccacgttat gtccctggtg gttcaagcgg tgggtctgca
     1441 gcagcggttg ctggagggtt agccgtcgca gcactcggga ctgacactgg ggggtccatt
     1501 cgcctgcccg caagctacac gggtacggtc gggttaaaac cgacttatgg gcgtgtaagt
     1561 cgaaacggtt tagtcgcttt tggtagctcg ctcgaccagg ctgggccgat gacgaccacc
     1621 gtttccgatg ccgccattgt cctggaatca attagcggtc acgatccgtt tgattcaacc
     1681 agcagtgtgc taccgagcga aaattttctt gcatcgatgg taacggagtc ttctagaaa
//
LOCUS       DAOMXO010000541         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40439_length_1739_cov_4.778504, whole genome shotgun sequence.
ACCESSION   DAOMXO010000541 DAOMXO010000000
VERSION     DAOMXO010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40439_length_1739_cov_4.778504"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLH57_10110"
     CDS             complement(<1..403)
                     /locus_tag="PLH57_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03007.1"
                     /translation="MNLVAIKKKIIPLCVMGLMAFPATSQAGLLSSCRELIDQGVSVV
                     KKTISQTISLGVKKTRDRINNSFPNAPRRFPKGTDQVSRVAEGQVDFESTTQVDLRPL
                     EPVSHFQSQLAKSALQYRATISAGDRTGAALL"
     gene            complement(540..1640)
                     /gene="nagZ"
                     /locus_tag="PLH57_10115"
     CDS             complement(540..1640)
                     /gene="nagZ"
                     /locus_tag="PLH57_10115"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRK03008.1"
                     /translation="MATTNEIKQMLGQSFFIGFAGLELPASVEKFIRENNIGGVTLFA
                     NNYESPAQVAELVNQIQRCNPETPLFIGVDHEGGRVQRFRKGFTRFPEARLIGDQDSP
                     KLAYECAQIMGTELKAVGVNLVFAPVVDINTNPKNPVIGRRSFGETEERVSKVSSGYV
                     RGFLNAGVQPVLKHFPGHGDTSQDSHFHLPTVRTSLETLRDRELKPFLKGFKAHANFV
                     MSAHIIVEAVEPKLPGTLSKKILQGLLREELRFKGIIISDDMEMKAIADHFGSVDAAC
                     MALEAGVNVLLYHTEPFQNEVWDPLLTKLTSPSGKQALVGLQDSAKRLAVAKRDYLNK
                     SQLVDIPAVEKLVGIDTHLALAKRLEEMDTKS"
BASE COUNT          407 a          388 c          458 g          486 t
ORIGIN      
        1 ttaatagtgc ggcgcctgtg cggtcgccgg cgctgatggt ggcacggtac tgaagtgcgc
       61 tcttggccag ctggctctgg aaatggctga ctggctcaag ggggcgtagg tctacctgag
      121 ttgtcgattc aaaatctact tgtccttcag ccacgcgcga aacctggtcg gtaccctttg
      181 gaaagcggcg aggcgcgttc ggaaatgaat tgttgatacg gtcgcgcgtt ttttttacgc
      241 ctaaagaaat ggtctgggag atagtttttt taacgaccga gacgccttgg tcaatgagtt
      301 cgcgacagga gctaagaagc cccgcttggg acgtagccgg gaaagccatg aggcccataa
      361 cgcacagagg gatgattttt ttctttattg ccaccaagtt cacttaattt atgcccctta
      421 ccgctgtttt tcgcacggcg ttaaatacca cgggacggtg gattgttgaa gcgaaatggt
      481 atagctttct aagtgtctga tatttattga gattttagag ttttaagagc aggggcgagt
      541 cacgatttcg tgtccatttc ttcgaggcgc tttgcgaggg ccaagtgggt gtcgattccg
      601 accagctttt caaccgctgg aatatctact agctgcgact tattgaggta atctcgcttg
      661 gcaactgcaa ggcgtttcgc tgaatcttga aggccaacca gggcttgttt accggacgga
      721 ctagtgagtt tagtaagtag tgggtcccag acttcgtttt gaaacggttc ggtgtgataa
      781 agtagcacgt tcacaccggc ttccagcgcc atacaggcgg catcgacgga gccgaagtgg
      841 tcggcaatag ctttcatttc catgtcgtct gaaataatta ttcctttgaa acgaagctct
      901 tcgcgcaaga gaccctggag gatttttttg gagagtgttc caggtagttt aggttcgact
      961 gcctcaacga tgatgtgtgc actcataacg aaattggcgt gggccttaaa gccttttaga
     1021 aacggtttaa gttctcggtc gcgaagagtt tcaagcgaag ttcgaaccgt aggtaaatga
     1081 aagtgagagt cttgagaagt gtcaccgtga ccgggaaaat gcttgagaac gggttgtacg
     1141 ccggcattga gaaagccgcg aacatacccc gatgacacct tggagacgcg ctcttcggtt
     1201 tctccaaaag accttctacc gattacgggg tttttgggat tcgtattaat gtccaccacc
     1261 ggtgcgaaca ccaggttgac acctacggct tttagttcag ttcccattat ctgagcgcac
     1321 tcataagcga gtttgggtga atcctgatca ccgatcaaac gagcttccgg aaagcgtgta
     1381 aagcctttac gaaagcgttg tacgcggccc ccttcgtgat ccacgccaat gaacaatggg
     1441 gtctcggggt tgcagcgctg aatttgattt acgagttcgg caacttgcgc aggagactca
     1501 tagttattcg caaatagtgt tacgccacca atgttatttt ctcgaataaa tttttcaacg
     1561 ctcgcgggca attcaagtcc agcgaatcca ataaaaaaac tttgaccgag catttgtttt
     1621 atttcattcg tagttgccat tttagtgtct gtccctgtgt tgaattttta gttagttcca
     1681 tccagaaaat ctagcgcttc tttctggttc gcgactcgac gaatcgagat agcttcttt
//
LOCUS       DAOMXO010000542         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40470_length_1738_cov_4.825906, whole genome shotgun sequence.
ACCESSION   DAOMXO010000542 DAOMXO010000000
VERSION     DAOMXO010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40470_length_1738_cov_4.825906"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>1738
                     /gene="argS"
                     /locus_tag="PLH57_10120"
     CDS             128..>1738
                     /gene="argS"
                     /locus_tag="PLH57_10120"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRK03009.1"
                     /translation="MMQRKTGKYESPFLAEVKSGVITAFSKINQGAVDEDLGRQISGL
                     IVQPPEFRLGQAALPCHQFAKKLRLPPVKIAEALKQELSAQSLKWIGQIDSVNGYLNF
                     HCRSEVLGEWLLNEIAGGGYFAKSLLAESEQEKMIVEYSQPNTHKALHVGHLRNTVFG
                     DAVCKVLHYAGHEIVRATYPGDMGAHIAKALWYVQTKKGGKLPPATENRADWLGVMYA
                     ESEACLRTAEKDDVDEFARIKIEIGKVLKELHSGKGPYYDLYLETREWSLGQMRDVYA
                     WLGVAFDIWYFESECDVPSKELVLNKFKEGFFSKSEGAVGIDLSQYGLGFAMYLKSDG
                     NGLYLTKDLELIRRKFEDPKITRSIYVVDARQKLHFQQLFKTAELMGYPQAAKSVHLS
                     YESVTDESGEPCSSRTLKGVRLSVLRDLLEGKVKKDYLERYRGEWTDEQINATAEAVA
                     LGALKYGFLRVDGQTVIRFVLDEWLKLDGDTGPYLQYVHARCCSVIEKVGRPKDLGHL
                     TLETEIEKELLYYLGRFNHYAAQAAREYR"
BASE COUNT          473 a          366 c          468 g          431 t
ORIGIN      
        1 aggttttcca actgtgcttc taccaccgat cgatcgactg gcatgtggag ggcccccttc
       61 cccctattgt aaacgcaggg cgtactatgt agaaggaaaa cttaatttac tcgaggttcg
      121 accaaaaatg atgcaacgca agaccggtaa atatgaatcg ccctttctgg ctgaagtaaa
      181 atcaggggta atcaccgcgt tttctaaaat taatcagggg gcggttgatg aagaccttgg
      241 tcgccaaatt tcagggctta tagtacaacc acctgaattt aggttggggc aggctgcttt
      301 gccttgtcat caatttgcga aaaaattgcg tttgccgccc gtcaaaatag cggaagcgct
      361 aaagcaggag ttaagtgcgc aatcgctcaa gtggattggt caaatcgatt cggtaaacgg
      421 ctatttaaac tttcattgcc gctccgaagt gcttggcgag tggttgttaa acgagatagc
      481 ggggggcggt tacttcgcaa aaagtcttct cgctgagtcc gaacaagaaa aaatgattgt
      541 cgagtattcc cagccgaaca ctcacaaagc gctccacgtc gggcatttac gcaacacggt
      601 gtttggtgat gcagtctgta aagtgttgca ttatgcaggg cacgaaattg ttcgagctac
      661 ttatccgggt gacatgggcg cgcacattgc aaaagcgctt tggtatgttc aaaccaaaaa
      721 aggcgggaag ttgccgcccg caacagagaa tcgagctgac tggcttggag taatgtacgc
      781 agagtcggag gcgtgtcttc gtacagcgga aaaagacgat gtcgacgagt ttgcgcgaat
      841 taaaatcgaa atcggaaagg tattgaagga actccactca ggcaaaggac cgtactacga
      901 cctctatctc gaaactcggg agtggtctct aggacagatg cgagacgtgt atgcttggtt
      961 gggagtggcg tttgatattt ggtatttcga aagcgagtgc gatgtccctt caaaggaact
     1021 cgttttaaat aaatttaaag aaggcttttt ttcaaaaagc gaaggggcag tcggaatcga
     1081 cctttcgcag tacggtcttg gttttgcgat gtacttaaag tcggatggga acggcttgta
     1141 tttaaccaaa gatttagagc tcattcgtcg aaagttcgaa gatccaaaaa taacgcgatc
     1201 aatttatgta gtagatgcac gtcagaagtt gcattttcag caactgttca aaacggcgga
     1261 attgatgggt tatccacaag ccgcgaagtc ggtgcacttg tcctatgagt cagtgacaga
     1321 cgaaagtggc gagccttgca gttcgcgcac tcttaagggc gttcgtctga gtgtgttgcg
     1381 cgatcttctt gagggtaaag taaaaaaaga ctacctagaa cgatatcgcg gtgaatggac
     1441 cgacgagcag attaacgcga ctgccgaagc agtagctctt ggggccttaa aatacggatt
     1501 tcttcgggtc gacggtcaaa ctgtaattcg ttttgtactc gacgagtggt tgaagctcga
     1561 cggcgacact ggtccctatc tgcagtacgt gcacgcccgg tgttgtagcg taatcgaaaa
     1621 ggtagggcgt cctaaagacc tggggcacct cactctagag accgaaatag aaaaagagct
     1681 tctgtattat ctgggccgat ttaatcacta cgcggctcag gcagctcgag aatatcgc
//
LOCUS       DAOMXO010000543         1736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40545_length_1736_cov_4.203450, whole genome shotgun sequence.
ACCESSION   DAOMXO010000543 DAOMXO010000000
VERSION     DAOMXO010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40545_length_1736_cov_4.203450"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1492)
                     /locus_tag="PLH57_10125"
     CDS             complement(41..1492)
                     /locus_tag="PLH57_10125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRK03010.1"
                     /translation="MHVSHWRALRFAAMAFALSFSAALAADTTEPVTKTPYAPGEVIV
                     KFKSDIRRNKVFMQQLYQEVGVAKVRKFKLGFGSIEHYQLAEGASVEKAIQKLTKTVA
                     VEYAQPNYILKLFRNEDVLDGYDDCHPPVEFLGCVPFGEFIEGRPGIAPKPSPVEPPV
                     ADPQQNKVWGLNAISAPEAWKVTTGSQGIVVAVIDTGIDYNHEDLSFNMWRRPGVESE
                     EDDIVGWDFVHDDNLPFDDHMHGTHVAGTIGAVGGNALGIVGVSPKVSLMAVKFLTAR
                     GSGTTLDAIRSIDYAVERGARILNNSWGGGADPNNNALRESIGRAEARGVLFVVAAGN
                     DGTDNDRRPTYPASFRAANLIAVAATDSNDKIASFGGGYGSNYGKTSVHLGAPGKDVY
                     SSVPGNRYRGASGTSMATPMVAGAAALLWSMNPNMTFLDVKNRLMATVDPLPTLQGKT
                     ITGGRINLARAVVTELDSGGEYDDEYDGEDGDW"
     gene            1649..1733
                     /locus_tag="PLH57_10130"
     tRNA            1649..1733
                     /locus_tag="PLH57_10130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Leu,seq:tag)
BASE COUNT          380 a          443 c          466 g          447 t
ORIGIN      
        1 atacagtcgt ccgccctttt tttaattccg gcacctgttt ttaccagtcg ccgtcttcac
       61 catcgtattc gtcgtcatat tcgccacccg aatcgagttc ggtaacgaca gccctagcaa
      121 gattaatacg accaccagtg atcgttttac cttgtagtgt agggagtggg tcgactgtgg
      181 ccatgagtcg gtttttgacg tcgaggaacg tcatgtttgg gttcatgctc cagagcagtg
      241 ctgccgcccc agcgaccatc ggagtggcca tcgatgtccc cgaagcgcct ctgtagcgat
      301 tgccaggaac tgagctgtaa acgtcttttc ctggagcgcc taagtgaaca ctggttttgc
      361 cgtagttcga gccgtaaccg ccgccaaacg aagcgatctt gtcgtttgag tcggtggctg
      421 caacggctat aagattggcc gcccgaaacg atgctggata ggttggtcta cgatcgttat
      481 cggtgccgtc gtttccggca gctactacga ataaaacccc gcgggcttct gcgcgtccaa
      541 tcgactcacg aagagcgttg ttgttcgggt ctgcgcctcc accccagctg ttgtttaata
      601 tgcgcgcgcc gcgttcaacg gcatagtcga tcgaacgtat tgcgtcgagt gtggttccag
      661 acccacgggc ggtgagaaat ttcacggcca ttagggaaac cttggggctc acgcctacga
      721 ttcctagagc attaccgccg actgcaccga tggtgcctgc gacgtgggtg ccgtgcatgt
      781 gatcgtcgaa agggagattg tcgtcgtgaa caaagtccca gcccacgatg tcgtcttctt
      841 cagattctac gcccggtctg cgccacatat taaaagatag atcttcgtga ttataatcga
      901 tgccggtatc gatcactgcg accacaatgc cttgcgagcc ggtggtgact ttccaagcct
      961 cgggagccga aatcgcgttt aagccccaaa ctttattttg ttgcggatcg gcgacagggg
     1021 gttcgacggg actgggcttg ggagcaattc ccggccgacc ttcaatgaat tcgccgaatg
     1081 gaacgcaccc aagaaattcc accggagggt ggcagtcatc gtaaccgtct agtacgtctt
     1141 cattgcggaa aagttttaga atatagttcg gctgagcgta ttccaccgcc actgttttgg
     1201 tcagcttttg gatagctttt tctaccgatg cgccttcggc cagttggtag tgctcaatag
     1261 acccgaaacc gagtttaaat ttgcgaacct tggctacccc aacttcttgg tagagttgct
     1321 gcataaacac tttgtttctg cggatatcgg atttgaattt tacgatcact tcgcccggtg
     1381 cataaggggt tttcgtgact ggttcggttg tgtctgccgc caaagcagca gaaaaagata
     1441 gggcaaacgc catagcggca aaacgcaggg cgcgccaatg agacacgtgc atagagatct
     1501 cctccataag agtagtttag gacgaacgtc agtgttcgct ttgtcgtcat tctatggcga
     1561 ttgggggttc ttgcaaacgg ttagataata aaaattaagt cattgctccc tgagacaagc
     1621 tatgattaga ttttgcctcg tttcgcacgc cgatgtggtg aaattggcag acacgctagg
     1681 cttaggacct agtgctgaaa agcgtggggg ttcgagtccc tccatcggca ccatta
//
LOCUS       DAOMXO010000544         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40576_length_1735_cov_5.723214, whole genome shotgun sequence.
ACCESSION   DAOMXO010000544 DAOMXO010000000
VERSION     DAOMXO010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40576_length_1735_cov_5.723214"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..819)
                     /locus_tag="PLH57_10135"
     CDS             complement(43..819)
                     /locus_tag="PLH57_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03011.1"
                     /translation="MDRRPILFLLFAFFLIVLPSNSHARKFTNQFVEFELPLKWDCVL
                     EGAEWVCQSTDENKRKDAIVVLAAKLKGDQDNLEKYQEYLGKPRQFTPPSGKTVTSQP
                     KYNRISQINSHSWVDALHMESEIPNFYTRYLATVKQDIGVLVTYSINKSKYQDYQTQF
                     DELVKSIRVFRKQGSGINTNVAGSIFNTTQPLNTYTAQGVFGNQAQASPVPGAESEKK
                     PAKSEGSDSDLLILIAIVAGVGGYIYLKKKRGGGGPPAGA"
     gene            complement(843..>1735)
                     /locus_tag="PLH57_10140"
     CDS             complement(843..>1735)
                     /locus_tag="PLH57_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03012.1"
                     /translation="DAGLEFPLYARLNAAEWARLDRTSEAPLGNGSALITLKGTLELP
                     IARRTVFGTSFGYTHRTEGYSSFLPYAAFFKFTEPNNFFGRAGITGTFHATGDQYTNQ
                     ILATERAKTVVAGSQAFNAIDPRFLALDLGAGIYLSPDIELAVGYQREISGRNSPVGH
                     HFLGALSWKIKTDSDVPAPHASNTPEQRAASNRGFYEYTGLAKVIRVNNALRLFLINQ
                     GRTHGIKVGEYLDVFSPKEATGDAPGDTIGKAFARARVVEAGPTRSKLEILETFPTGK
                     DNNGNPIPLREGFVVRRPLP"
BASE COUNT          417 a          403 c          444 g          471 t
ORIGIN      
        1 acaagaattt cgtgagaaat atctaaatta agcgagacca ggtcatgcgc cggctggagg
       61 cccgccgccg ccgcgtttct ttttcaaata aatgtacccg cccactcctg ctactatcgc
      121 aattaagatc aacagatcac tatctgaacc ttccgattta gccggttttt tttcgctctc
      181 tgcgccaggt actgggctag cttgggcctg atttccaaaa acaccctgcg ctgtgtaagt
      241 attgagcggc tgagttgtgt tgaagatcga acccgcgaca ttggtgttaa tgccgcttcc
      301 ttgcttgcgg aacacacgaa tactttttac gagctcatca aattgggttt gatagtcttg
      361 gtatttcgat ttgttgatgg aatacgtcac gagcacgcca atgtcttgtt ttacggtcgc
      421 taaatagcgc gtataaaagt tagggatttc ggactccatg tgcaaagcat caacccaaga
      481 gtgagaattg atttgggaga ttcggttgta cttcggttgg gaagtcacgg tttttccgct
      541 cggaggagtg aactgacggg gctttccgag gtattcctga tatttctcta aattgtcttg
      601 gtcgcctttg agtttggcag cgagaaccac aattgcatct tttcgtttat tttcgtcggt
      661 gctttgacac acccactcag ctccttccaa cacacagtcc cacttgagcg ggagctcaaa
      721 ttcgacaaat tggtttgtaa atttgcgcgc gtgggaattg gacggcaaaa cgataagaaa
      781 aaaggcgaat agcaaaaaga gaatgggacg tcgatccatg gcgatgttct cctcttttca
      841 ttctaaggta gagggcggcg aactacaaat ccttctctca aaggaatagg atttccgtta
      901 ttatcctttc ctgtcggaaa ggtttctaaa atttcgagtt ttgaccgcgt aggccccgcc
      961 tcaacaacgc gagctcgagc aaaagctttt cctatagtgt cacctggagc atcgcctgtc
     1021 gcctctttcg gcgaaaacac gtctaagtat tcgccgacct tgatcccgtg agtgcgacct
     1081 tgattaatta aaaacaaacg caatgcatta tttacgcgga ttactttcgc taggccggtg
     1141 tattcgtaga atcctcgatt agaagcggcg cgctgttctg gtgtgttgga agcgtgaggg
     1201 gctgggacat cgctatctgt tttaattttc caagacaagg cgcccaagaa atggtgcccc
     1261 acaggtgaat tgcggccaga aatttcgcgt tggtaaccga ctgcgagttc gatgtccggg
     1321 cttaaataaa ttccggcacc caaatcgagg gctagaaaac ggggatcaat tgcgttgaat
     1381 gcttggctgc cggccaccac tgtttttgcc cgctcggtcg cgagaatttg atttgtgtat
     1441 tggtctcccg tggcatgaaa agttccagta atgccggctc ttccgaaaaa attgtttggc
     1501 tcggtgaatt tgaagaaagc ggcgtagggg agaaaacttg agtacccttc ggtgcggtgg
     1561 gtgtaaccaa aacttgttcc gaaaactgtt ctccgcgcaa tcggaagctc aagtgttcct
     1621 ttaagagtga tgagagcact gccgtttcct aggggagcct ccgacgtgcg gtcgagacgg
     1681 gcccactcgg ccgcgtttaa tctggcgtaa aggggaaact cgaggcccgc atcaa
//
LOCUS       DAOMXO010000545         1734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40615_length_1734_cov_5.018463, whole genome shotgun sequence.
ACCESSION   DAOMXO010000545 DAOMXO010000000
VERSION     DAOMXO010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40615_length_1734_cov_5.018463"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1087
                     /locus_tag="PLH57_10145"
     CDS             68..1087
                     /locus_tag="PLH57_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03013.1"
                     /translation="MFRGIFSFFGFFLLSLSASSIEREFEWHEGWTGVISDREECEFD
                     SIRNALNHSCVSEFNSIAEKLTQDRFRISKKGLATRRDIEKVVLLLHSEEALSFYERL
                     YSKYAQSFTRSASVLSPLPDNLSGMDAMVQRAEEKEASGFSDLAYLLSSKVFGSDKPA
                     VYCGLLKGSFRGYASQFSSVNQVVFVLICREAQKKEMDWQGTESAAETELREKLDSEA
                     PFRVEVVLRVPRTEDGSLPEFVPRDSLAITAEYRIRGNKSAVVGSTKYWNYQLYSIES
                     KFDLRFATEPILKTVLQYETLPKEKLNSYLNKVPYYFETETGFCDALGSFIVKELNAS
                     QKNCQ"
     gene            complement(1103..>1734)
                     /locus_tag="PLH57_10150"
     CDS             complement(1103..>1734)
                     /locus_tag="PLH57_10150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HRK03014.1"
                     /translation="GYQYSANDPFALLWIHATHWYAVLRTYEVFVRTLTDAERNVYIG
                     ETKIFAMLLGIPENMIPSNWSEFKTYFDTMVSSPELTYSRDARALEQHFRVMSREVER
                     KNPAVWIARKYSDAITASTLPKKLAEQFDFERSRTKNALYVAALSALRFSYPLIPRWG
                     GELPLYRMAMARARGAAPDPKDELLYKAFMGRANFYQFAQEAAKIALKR"
BASE COUNT          446 a          361 c          450 g          477 t
ORIGIN      
        1 aaccgggcct gtggaattga tgcctcgact gtggtagccc cctctttggt tatggggagg
       61 ggctacggtg tttcggggta tattttcttt ttttgggttt tttctacttt ctctttcggc
      121 gagttcaata gaacgagagt tcgagtggca cgaaggttgg acgggcgtaa tatctgaccg
      181 cgaagagtgt gaattcgata gtattcgtaa cgcactgaat cattcctgcg tatcagagtt
      241 taattcaata gccgaaaaat taactcagga tcggtttcga atttcaaaaa aaggtctggc
      301 aacccggcgt gacatcgaga aggtggtgtt gctccttcac agtgaagaag cgctttcttt
      361 ttatgaacga ctctatagca agtacgctca aagcttcacg cgttcggcta gtgtactctc
      421 gccgctacca gataatctca gtgggatgga tgcaatggtg caacgagctg aagagaagga
      481 agcgagcggc ttctcggacc ttgcttattt actgagttcg aaggtttttg gtagcgataa
      541 accggcagta tactgtggtc ttttaaaggg aagtttccgt ggctatgcga gtcagttcag
      601 ttcggttaat caggtcgttt tcgttttgat ttgtcgtgag gcccagaaga aagaaatgga
      661 ttggcaagga accgaaagtg cagcggaaac ggaacttcgg gaaaaacttg attccgaagc
      721 cccatttaga gtggaggtgg tgctgcgggt tcctcgaacc gaagacggta gtttgcccga
      781 gtttgtgcca cgggactctt tagctataac tgcagaatac agaatacgcg gaaacaagtc
      841 tgctgttgtt ggatcgacca agtattggaa ttatcaattg tacagtatcg agtctaagtt
      901 tgacttgaga tttgctacag agcctattct taaaacagtg ttgcagtacg aaacgcttcc
      961 taaagaaaaa cttaattcct atttaaacaa agttccgtat tacttcgaaa cggaaacagg
     1021 tttttgcgat gcgctcggaa gttttatcgt aaaagaatta aatgcgagtc agaaaaactg
     1081 ccaataggta tcggtaattg aactatcgct ttaatgctat tttcgccgct tcctgggcaa
     1141 actgataaaa attggctctg cccatgaaag ctttgtaaag tagttcgtcc ttagggtcag
     1201 gggccgcgcc acgtgctcgc gccattgcca tgcgatagag gggaagttcg ccgccccatc
     1261 taggaatcaa agggtatgaa aaacgaagcg cgctcaaagc cgctacgtac aaagcatttt
     1321 tggttcggga tcgctcaaag tcgaattgct ccgccagttt ttttggcaac gtgctggccg
     1381 taatcgcatc agaatacttt cgtgcaatcc aaactgcggg gtttttacgt tcgacttcgc
     1441 gactcatgac ccggaaatgt tgctcgagcg cgcgtgcatc gcgcgaatag gtgagttcgg
     1501 gtgaacttac catggtgtcg aagtaggttt tgaattcact ccagttggac gggatcatgt
     1561 tttccggaat acccaacagc atggcaaaaa tttttgtctc gccaatgtaa acgtttctct
     1621 ccgcatctgt gagtgttcgt acaaacactt cgtaggtgcg gagtaccgcg taccaatgag
     1681 ttgcgtgaat ccacaacaac gcaaaaggat cgttggcgct gtattgataa cccg
//
LOCUS       DAOMXO010000546         1732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40675_length_1732_cov_5.660107, whole genome shotgun sequence.
ACCESSION   DAOMXO010000546 DAOMXO010000000
VERSION     DAOMXO010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40675_length_1732_cov_5.660107"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="PLH57_10155"
     CDS             <1..1379
                     /locus_tag="PLH57_10155"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRK03015.1"
                     /translation="HRCTDFEMAQKKFLGDGVVTGFGTIDGRKVYVFSQDFTVFGGSL
                     SGAVANKICKIMDLAVKAGCPLIGLNDSGGARIQEGVESLGGYAEIFWRNTQASGVIP
                     QISVILGPCAGGAVYSPAITDFIFMVENTSYMFVTGPDVIKTVTHESVTKEELGGAHT
                     HASKSGVCHFAYPNEVSTLNAVRELVAYLPANNLDVAPAAEKRDPSDRDCAKIAGVIP
                     QNSRKPYDMRAVITDVVDDGRFFEVHKDFAGNMTVGFARLDGRTIGVIGNQPAVLAGC
                     LDIDASTKAARFVRFCDAFNIPLLTFVDVPGFLPGTNQEFGGIIRHGAKLLYAYAEAT
                     VPKVAVITRKAYGGAYDVMSSKHIRADFNFAYPSAEIAVMGAEGAVNILYKREIEAAK
                     DKDSERKKRIAEYEEVFANPYKAAELGYIDAVIEPAQTRRALCQAFDVLKSKRERGPA
                     RKHGNLPL"
     gene            1449..>1732
                     /locus_tag="PLH57_10160"
     CDS             1449..>1732
                     /locus_tag="PLH57_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03016.1"
                     /translation="MQTRPWSLVVLAFFHFLTPVVSVVINCYFLQVNPIELMGIIWDQ
                     GVREMIEFAILPFVAGFAIYKTKKWSYPVFIAAMLWISVSNYFAWRSSEQV"
BASE COUNT          444 a          359 c          458 g          471 t
ORIGIN      
        1 ctcatcgttg tacggatttc gaaatggccc aaaagaaatt cttgggcgat ggtgtagtaa
       61 cagggtttgg cacgatcgat ggtcgtaaag tatacgtttt ttcgcaagac ttcactgtgt
      121 ttggcggaag tttgagcgga gcggtagcga acaaaatttg taagatcatg gaccttgccg
      181 ttaaggctgg ctgtccgtta attggactca atgacagcgg gggcgcccgc attcaagagg
      241 gagttgagtc gttaggcggt tatgctgaaa ttttttggcg aaatactcaa gccagcggag
      301 tgattcctca aatatcggtg attctcggac catgtgcggg tggtgcggtt tacagtcctg
      361 cgattactga tttcattttc atggtggaaa acactagtta catgttcgtg acaggtcccg
      421 atgtcatcaa aacggtgact catgaaagtg tgacaaaaga agaattgggt ggagctcata
      481 ctcacgcgtc caaatcgggt gtttgccact ttgcgtaccc aaacgaagtt tccacgctta
      541 acgcggttcg tgagcttgta gcttatttac cagcaaataa cttagatgta gcacccgctg
      601 ccgaaaaacg ggatccgtcg gatcgggatt gtgcgaaaat cgcgggagta atacctcaga
      661 actctcgtaa accctacgat atgcgggccg taataactga cgtggtcgat gacggtcgtt
      721 tttttgaagt tcacaaagac ttcgcgggaa atatgacggt aggtttcgct cgactagatg
      781 ggagaaccat tggtgtcatt ggaaatcaac cagctgtatt agctggttgt cttgatattg
      841 atgcttctac taaagccgct cgatttgtgc ggttttgcga tgcctttaat attccgcttt
      901 tgacgtttgt tgatgtgcct ggttttttgc ctggcacgaa tcaagagttt ggcggaatta
      961 ttagacacgg cgccaaactc ttatatgcct atgctgaagc gactgtgccg aaagttgcag
     1021 tcattactag aaaagcttac ggcggtgctt atgacgtgat gtccagcaaa catattcggg
     1081 ccgactttaa tttcgcatat ccatcggccg agatcgcggt gatgggggct gagggagccg
     1141 tgaacatatt gtataaacgc gaaattgagg ctgccaaaga taaagacagc gaacgaaaga
     1201 agcggattgc agagtatgaa gaagtttttg caaaccctta caaggcagcg gagttgggtt
     1261 acatcgacgc agtcattgaa cctgctcaaa cacgtcgcgc actgtgtcag gcgttcgatg
     1321 tattaaaatc caaacgcgag cgagggccag ctcgaaagca cggaaaccta cctctctaat
     1381 cggttcttgt tcggatcaat acgctccggt aaactaagca ctagggaata ataaaaaagg
     1441 ggctttccat gcaaacccga ccttggtctc tagtcgttct ggcgttcttt cactttttaa
     1501 ctccggttgt gagcgtagtg atcaactgct actttctgca ggttaatccc atagaattga
     1561 tggggattat ttgggatcaa ggcgtccgag aaatgatcga attcgcaatt ctgccattcg
     1621 tagcgggttt cgcgatctac aagacgaaaa agtggagcta cccagttttt attgcggcca
     1681 tgctttggat atcggtttcg aattacttcg cgtggagaag ttccgaacaa gt
//
LOCUS       DAOMXO010000547         1727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40873_length_1727_cov_3.002990, whole genome shotgun sequence.
ACCESSION   DAOMXO010000547 DAOMXO010000000
VERSION     DAOMXO010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40873_length_1727_cov_3.002990"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PLH57_10165"
     CDS             <1..442
                     /locus_tag="PLH57_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK03017.1"
                     /translation="FALEAKKPALDARFTSASFNIFLLLGDVYFDRGEFIEAFSYYRN
                     AVEEAKSRHAKAAALVSAAYAAENLKNSDEAIAILRQVVASGVKEVKADAMLALARNL
                     FQKGDRDGAVKQLNEVSKEFPNTVWATSAEGFQKLWTSEKSVSP"
     gene            412..1584
                     /locus_tag="PLH57_10170"
     CDS             412..1584
                     /locus_tag="PLH57_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRK03018.1"
                     /translation="MDFRKISLTLVAFALPGILACGSRNLHKNTDLSKAGGSPIERTF
                     VKTSHPENPDAGVERVEFGAAVSTADAVFTANEVEGVAAFFRSNFAKKWTFRVKNGVS
                     SALLLAEDGAGGHTLFFGANDGYFYALDAEFGRILWRYETKLPVYAKPTLAGGKIYFT
                     TSDEVVFCLDQNSGKWFWHYKRTHNIGTSIRGNSSPTIDGKHIIVGFSDGYVVALGAK
                     DGNLVWERKIHSGKRFMDVDASAVADGSTLFMPSYDGGLYALDRTTGKILWKVDAGSA
                     KKVLLDPRDSKSLYVAASDGTVVALNRSSGRILWKFELDAGTPTELVLKDNYLAFGSS
                     VQYFYVIHKGDGSLAYRHNVGLRSGFVSNPFQSENELFLMSNFGNLYVFRWVKKNG"
     gene            1577..>1727
                     /locus_tag="PLH57_10175"
     CDS             1577..>1727
                     /locus_tag="PLH57_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRK03019.1"
                     /translation="MAEMDLLIALATLSAMEIVLGIDNLVFIAILVGRLPKNRQPLAR
                     RIGLIG"
BASE COUNT          435 a          406 c          428 g          458 t
ORIGIN      
        1 cttcgctttg gaagcgaaaa agcctgccct cgatgcacgt tttactagcg cctcatttaa
       61 tattttcttg ttgttgggtg atgtttattt tgatcgcgga gaattcattg aagccttttc
      121 ctattatcga aacgctgtcg aagaagctaa atcacgtcac gcgaaagctg cggcgttagt
      181 ttcggctgcc tatgccgcag aaaatctaaa aaactctgac gaagcgattg ccattcttcg
      241 ccaagtagtt gcatcaggcg taaaagaagt aaaagccgat gcaatgttag ccttggcgcg
      301 caatcttttt caaaagggtg accgcgatgg cgcagttaaa caactaaacg aagtatctaa
      361 agagtttcca aatactgttt gggccacatc agctgagggc tttcaaaaac tatggacttc
      421 cgaaaaatca gtctcacctt agtagcgttt gctttacctg gcatcttggc ttgtggctcg
      481 cgcaatttgc acaagaacac cgatctctct aaggcgggtg gttctccgat tgaacgcaca
      541 ttcgtaaaaa cttctcaccc agaaaaccct gacgcaggag ttgaacgggt agagttcgga
      601 gcggccgtgt ctactgcaga cgccgttttc accgccaacg aagtagaagg cgtcgcggct
      661 ttttttcgat cgaacttcgc caagaaatgg accttccgtg taaaaaatgg cgtgtcaagt
      721 gcgctgctat tggccgaaga cggggcaggg ggccacactc tattctttgg agctaacgac
      781 ggttattttt acgcgctcga cgccgaattt ggacgcattc tttggcgtta tgaaacaaaa
      841 ctcccggttt acgcaaaacc aacccttgct gggggcaaga tttacttcac aaccagtgac
      901 gaagtagtct tttgcttgga ccaaaacagc ggaaaatggt tttggcatta taaacgcact
      961 cacaatatcg gcacttcgat tcgcggtaat tcgtcgccga caatcgacgg gaagcacatt
     1021 atcgtaggtt tttctgacgg ctacgtcgtt gctctaggag ccaaagacgg caacctagtt
     1081 tgggaacgca aaattcattc gggaaaacga ttcatggacg tcgacgcatc ggcggtggct
     1141 gatggtagta cgctttttat gccctcttac gacggtggat tgtatgcact ggaccgcacc
     1201 actggaaaaa ttttatggaa agtggacgct gggagcgcga aaaaggttct gctagatccc
     1261 cgcgactcca agagccttta cgtggccgcc agcgatggta ctgttgtcgc tctcaatcgt
     1321 agctcgggtc ggatattgtg gaaattcgaa ctcgacgcgg gaactcccac tgaattggtt
     1381 ctcaaagaca actacctggc ctttggtagt tccgtgcaat atttttatgt gattcacaag
     1441 ggtgacggat cgctcgctta ccgacacaac gtcggccttc gcagtggttt tgtatcgaat
     1501 ccttttcagt cagaaaatga attgttcctc atgtcgaact tcgggaacct atacgttttt
     1561 cgctgggtaa agaaaaatgg ctgagatgga cctgctgatt gcgcttgcaa cgctttcggc
     1621 aatggaaatt gtactcggaa tcgacaacct cgtctttatc gcaattttag ttgggcgatt
     1681 gccaaaaaat cggcaacctc tcgcacgtcg catcggtctt attggtg
//
LOCUS       DAOMXO010000548         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_40918_length_1725_cov_4.351497, whole genome shotgun sequence.
ACCESSION   DAOMXO010000548 DAOMXO010000000
VERSION     DAOMXO010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40918_length_1725_cov_4.351497"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLH57_10180"
     CDS             <1..230
                     /locus_tag="PLH57_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03020.1"
                     /translation="YSDFSALVAKIVNESQRFTEARVLSAHQGAWEAEIAREVYAAHG
                     LRVIEKKIDSAHTSSLWPSPYDTFVFSVLVR"
     gene            289..1119
                     /locus_tag="PLH57_10185"
     CDS             289..1119
                     /locus_tag="PLH57_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03021.1"
                     /translation="MSWIVLAATAHSQTIELNSEFDDRYAEHLRTQNNSVRLEPRTFA
                     VRAFRTKKKQRRPRVFSYERYVLEQIFAYFNAYAREFHISTDRNFARMGDFHFQAVRN
                     YRKVLPSQFALYLDIWETVVFDNQLGVKSKGGAAGLRYTYRPNRKSGVSLSVAPIAIE
                     DLTYYDGSSYLSFGSDRFYRHSSALELRHLLRGSTEASARMVFEPALLDFGEFRVYVE
                     TYARFTAYKNQHRHRHGVRLEEIKVIPKVVYIDHPQTGTPLDRNFSFFTKLEFRFQAQ
                     "
     gene            1129..>1725
                     /locus_tag="PLH57_10190"
     CDS             1129..>1725
                     /locus_tag="PLH57_10190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK03022.1"
                     /translation="MPRHSIVIPVFNERDGLEELFLRMKEVADQIVDSSSDDAEIILV
                     NDGSKDGSLEKLDEIATRDNRFKVLHLSRNFGHQVAITAGLEWASGDTVTVMDADLQD
                     PPEVILEFIQKWREGFEVVYAVRRSRAGETKFKLWTAKLFYRIIRRITQFDIPVDTGD
                     FRLMDRRAADAFLKLRERHRFIRGLVSWIGYKQVGVPYD"
BASE COUNT          407 a          412 c          449 g          457 t
ORIGIN      
        1 aatattcaga tttcagcgcc ctcgtggcaa aaattgtgaa tgagtcgcaa cgttttacgg
       61 aggctagagt gttgagtgcc catcaagggg catgggaagc agagatcgcc cgggaggtgt
      121 atgccgcaca tggactacga gtaatcgaaa aaaaaatcga ctcggctcac actagtagtc
      181 tttggccttc gccctacgac acatttgtgt tctcggtttt agttcgatag atttggagcg
      241 atgaacctgc ggcggcggaa ctgcgttcgc gtgatgtgtt gggggttttt gagttggatt
      301 gttcttgcag ccaccgctca ttctcaaaca atcgagttga acagtgagtt tgacgatcgc
      361 tatgccgaac atttgcgaac tcaaaacaac tcagttcggt tggaaccgcg aacttttgcg
      421 gtccgagctt ttcgtaccaa aaaaaaacag cgccgtccga gggtgtttag ctacgaacga
      481 tatgtcttgg aacaaatctt cgcgtacttc aacgcctatg cgcgagaatt ccacataagt
      541 acggatcgca attttgcacg aatgggggat tttcatttcc aggcagtgcg aaactaccga
      601 aaagtcctac cctcgcagtt cgcgctttat ctcgatattt gggaaaccgt ggttttcgat
      661 aatcagcttg gcgtaaaatc caagggtggt gcggccggcc tgcgctacac ctatcgtccg
      721 aatcgaaagt cgggtgtgag cttgtctgta gctccgatcg caattgagga cctcacttac
      781 tatgacggga gctcctattt gtcgtttggt tctgatcgat tctatcggca ctcctcggcg
      841 ctagagcttc gccacttgtt gcgtggatcc acggaggcca gtgcccgaat ggttttcgag
      901 cccgcactgc tcgatttcgg agagtttcgc gtttatgtcg agacctatgc tcggtttacg
      961 gcctataaga accaacaccg ccatcgacac ggtgttcggc tcgaggagat caaagtgatt
     1021 cccaaggtgg tttacatcga ccaccctcag acgggcaccc cgcttgatcg gaatttttcg
     1081 tttttcacga agcttgagtt tcgctttcag gctcaataga ataaccccat gcccagacac
     1141 tccatcgtga ttccagtgtt caatgaacga gacggccttg aagaactatt tttacgcatg
     1201 aaggaggtgg ctgatcaaat tgttgattct tcgagcgatg acgccgaaat aattttagta
     1261 aacgacggta gtaaagatgg ttcgcttgaa aaactcgacg agattgcgac tcgcgacaac
     1321 cgctttaaag tattacatct ttcccgaaac tttggtcatc aagtcgccat cacagctggg
     1381 cttgagtggg ccagcggcga tacggtcacc gtcatggacg cagatttgca ggatccaccc
     1441 gaggttattt tagagttcat tcaaaagtgg cgtgaaggtt ttgaagtggt ctatgccgtg
     1501 cgccgttctc gcgccgggga gacgaagttt aaactctgga ctgccaagtt attttaccga
     1561 attattcgcc gaatcacgca gttcgacatt ccagtcgaca ctggagattt tcgcctcatg
     1621 gatcgccgag ccgccgatgc gttcttaaag cttcgggaac gccaccgttt tattcgcggt
     1681 cttgtgagtt ggatcggata taaacaagtg ggggtgcctt acgat
//
LOCUS       DAOMXO010000549         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_41231_length_1716_cov_3.629741, whole genome shotgun sequence.
ACCESSION   DAOMXO010000549 DAOMXO010000000
VERSION     DAOMXO010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41231_length_1716_cov_3.629741"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PLH57_10195"
     CDS             <1..937
                     /locus_tag="PLH57_10195"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HRK03023.1"
                     /translation="IDEIQRIPRLLNSIQALIDRDKKLKFYLTGSSARKLKRGKANLI
                     PGRVFSYALAPLCLAEAGRDWDEKLALQFGSLPEVTSLSSVADKKKILQSYTNAYLKE
                     EILSEALVRQVDGFVRFLTQAGAQAGRYLDYSKLAARAKVPRQSVVRHFEILEDTMIA
                     HRVENDPDLDPDQVDLVKHPRFYFFDLGVLNALRGTFDLGSDRIGALFEHLVFNQLFN
                     SATAHAVDFRIHNFRTRGGLEVDFILALEGRKVAIECKASDHVSDVEIAPLRALSTYY
                     KKMEKMVIYRGLRERKNQDVWILPLPRALQVMGLL"
     gene            complement(943..>1716)
                     /locus_tag="PLH57_10200"
     CDS             complement(943..>1716)
                     /locus_tag="PLH57_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03024.1"
                     /translation="GTAENRSMTICLSASGHKAEQARGNDIINAFDPVIEIESELDSV
                     YSKIKANLTKAYENIPSIAFDPVVSHCDVIDGMSNYTNSHITLCTWKYLVDLNAEMKG
                     ALKNIKNGAQPLVELSTRLMIKYPEIYEKMIKSCVQKNVDEKCLKNFAGRLGRDKMIE
                     SIDGFRRTLVLDTWLKTLQNDISRRSAHGKKFRDLNRSYASHYIGDFDGEAVALEDKM
                     PKKSNKKKGPKKKIATAATSSSKNEPSTRSVNPPAAAVD"
BASE COUNT          409 a          396 c          416 g          495 t
ORIGIN      
        1 catagacgag attcaaagaa ttccccgtct attgaattcc attcaggcgc tgatcgatcg
       61 tgacaagaag ttaaaatttt acctcactgg ttcgagcgcg cgaaaactaa aacgtggaaa
      121 agcgaatctg attccgggcc gagtgttttc ttacgcgttg gcaccgcttt gcttggcaga
      181 agcgggtagg gattgggatg agaaactggc cttgcagttc ggtagcctcc ccgaagtcac
      241 ttcgctttcg tcggtcgccg acaaaaagaa gattttgcaa tcgtatacca atgcctattt
      301 gaaagaagag atcctgtccg aagccttggt gcgccaggta gatggttttg tgcgtttcct
      361 tactcaagcc ggtgcacaag cagggcggta cctcgattat tccaaactcg ccgcgcgagc
      421 caaggtgcca aggcagtcgg tcgttagaca tttcgagatc ctcgaagaca cgatgatcgc
      481 ccaccgggtt gaaaatgacc ctgatttaga cccggatcag gtcgaccttg taaaacaccc
      541 acggttctat ttttttgatt tgggcgtgct caacgccctt cgtggtacct tcgatttggg
      601 tagcgatcgg atcggagcat tgttcgagca tttggtattc aatcaactct ttaattcagc
      661 taccgcgcac gcggtggatt ttcgaatcca taattttcgc accaggggcg ggcttgaagt
      721 tgatttcatc ctcgcattgg aaggccgaaa agtagcgatc gagtgtaaag ctagcgatca
      781 tgtctcggac gtggaaattg ccccgctgcg tgccttgagt acgtattaca aaaaaatgga
      841 aaaaatggtg atctaccgtg gtttgcgaga acgtaaaaac caagacgtct ggattttacc
      901 tctacccagg gccctacagg tcatgggact tctttaaaca acttaatcca ctgcggcagc
      961 aggtggattc actgaccggg ttgatggttc gttttttgaa ctgctggtgg cggccgtagc
     1021 tatctttttc tttggtcctt ttttcttatt acttttttta ggcattttgt cctctaaggc
     1081 cactgcttcg ccatcgaaat cgccgatgta gtgggaggcg taacttcggt tcaagtcgcg
     1141 aaactttttc ccatgagcag atcttcgact gatgtcattt tgcagcgtct tcaaccaagt
     1201 gtcgagtacc agggtccggc ggaatccatc gatcgattca atcatcttgt ccctacccag
     1261 gcgaccggca aagttcttaa gacatttttc gtccacattt ttttggacac acgacttgat
     1321 cattttttcg taaatctcgg ggtacttaat cattaacctg gtactaagct cgaccaatgg
     1381 ttgggcaccg tttttgatgt ttttgagtgc gcctttcatt tcggcattga gatcaacgag
     1441 atacttccaa gtacaaagag tgatgtgcga attggtgtaa ttagacattc catcgatgac
     1501 gtcgcagtga gaaactacag ggtcgaaagc aatcgatgga atattttcgt aggccttagt
     1561 gagatttgct tttattttcg aataaaccga atccaattcg ctctcaattt cgatgacggg
     1621 gtcgaaagcg tttataatat cattcccgcg cgcttgctcg gctttatgac cgcttgcact
     1681 taagcaaatg gtcatgcttc gattttcagc ggttcc
//
LOCUS       DAOMXO010000550         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_41323_length_1713_cov_6.430639, whole genome shotgun sequence.
ACCESSION   DAOMXO010000550 DAOMXO010000000
VERSION     DAOMXO010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41323_length_1713_cov_6.430639"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="asnB"
                     /locus_tag="PLH57_10205"
     CDS             complement(<1..924)
                     /gene="asnB"
                     /locus_tag="PLH57_10205"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRK03025.1"
                     /translation="MSSAIAHRGPDGSGLKTWSTTALAHRRLSILDLSENASQPMRYS
                     DSGLWITYNGEIYNYLEIRSELESLGYSFRTNSDTEVILAAFDRWDISAFNRFNGMWA
                     LAIYDERKQRLVLSVDRFGVKPLYYMTTADGGIIFASELKAFFAVARKLNLKWDEKGI
                     RTALLSPLKLEGTGHTVIKNLSSMGGGEALDASPKSINKIQWWNTADHIEANFKEPRH
                     NADEFLNLLTDAVRIRLRSDVPVASSLSGGLDSSAIVAILSRLGSAQNQTAFVHAFEN
                     TRHDESNFAKKTIAHTGVLGKWVTLDETNLVN"
     gene            complement(948..>1713)
                     /locus_tag="PLH57_10210"
     CDS             complement(948..>1713)
                     /locus_tag="PLH57_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRK03026.1"
                     /translation="KTKTRERPNFSHIRNDTIQLAKQSWPLGLSSLLVLSYSKLDLIL
                     INSLGSPNETGIYAAGSKILEYLGVFPSIFSISILPLIFSVFRNHEAGSQKERERVSM
                     AIGVANILAWIIPIAMYALAPWIITLLYGTAFREAVSILRTIVWIFPLTLFGVVRQGF
                     LLLNEKLSICIALEALTLALLVGFNYLLIPKHGAQGSATALLLTSLMVNIAAIVVSKS
                     VRNIAKFYIKSIWSCRRFRLTNIKRCVESLGIFKPH"
BASE COUNT          474 a          386 c          402 g          451 t
ORIGIN      
        1 gtttacgaga ttggtttcgt cgagagtaac ccattttcct aaaacccctg tgtgggcgat
       61 tgtttttttt gcgaaattgc tttcgtcgtg tctggtattt tcgaaagcgt gcacaaaggc
      121 agtttgattt tgcgctgaac ctagccgact taagattgct acgattgcac tggagtcgag
      181 tccgccactt agcgaagaag cgacagggac atcgcttcgt aagcgaattc gcaccgcgtc
      241 agtaagaagg ttcaaaaatt cgtcggcatt gtgtcgaggc tctttaaaat tcgcctcaat
      301 gtgatcggcg gtattccacc actgtatttt atttattgat ttcggactag cgtctagagc
      361 ctcccctcct cccatactcg agaggttctt aataacggtg tgccccgtac cttcaagttt
      421 gagtggcgac agcagcgcgg ttctaattcc cttttcgtcc cattttaaat ttaattttct
      481 cgccaccgca aagaatgcct ttagttccga tgcgaagatt attccaccgt cggcggtcgt
      541 catgtaatag aggggtttta ctccgaatcg atcgaccgac aaaaccagac gttgttttct
      601 ttcatcataa atagcgagcg cccacatacc gttgaagcga ttaaaggccg agatatccca
      661 acgatcgaat gcagcgagta ttacttctgt gtctgaattg gttcgaaagg aataacccaa
      721 ggattctaat tcgctgcgaa tctctaggta gttatatatt tcaccgttgt aggtaatcca
      781 cagaccagag tcagaatatc gcataggttg cgacgcgttt tcggagagat ctaatatgga
      841 gagacgtcga tgggcaagtg ccgtagtgct ccaagtcttg aggccactac catcgggccc
      901 gcgatgggcg atagcggagc tcatttgagc caggtcgtga ggatttatta atgcggtttg
      961 aaaataccca gcgattccac acatcgtttt atgtttgtta gccgaaaacg tcggcaactc
     1021 caaatggact ttatgtaaaa cttcgctata ttgcgcaccg acttcgaaac aacgatggcc
     1081 gcaatattta ccattaaact cgtcaacaaa agggcagttg cagagccttg cgcgccatgt
     1141 ttcggaatta ggaggtagtt gaagcctacc agtagcgcga gcgtcaacgc ctcaagggca
     1201 atacaaatac tcaacttctc attgagaagt agaaagcctt gacgcacaac gccaaataag
     1261 gtcaacggaa agatccatac gatagttcgc aatattgata cggcctcccg aaaggcggtt
     1321 ccgtagagta gtgtgataat ccaaggagct agagcataca tcgcgatggg aataatccag
     1381 gcaagaatat tagcgacccc aatggccatt gacactcgct cgcgctcttt ctgagaacct
     1441 gcttcatgat ttcgaaaaac agaaaatatg aggggtagga tcgaaattga aaaaattgaa
     1501 gggaacaccc ccaaatactc taaaatcttg gaaccggccg cataaattcc agtctcattg
     1561 ggagagccca aagaattgat caaaataaga tcgagcttcg aataactgag tactaaaagg
     1621 gacgataatc ccaagggcca actttgtttt gcgagctgta tagtatcatt gcggatatga
     1681 gaaaagttgg gtctctcccg agttttggtt ttc
//
LOCUS       DAOMXO010000551         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_41474_length_1709_cov_4.337969, whole genome shotgun sequence.
ACCESSION   DAOMXO010000551 DAOMXO010000000
VERSION     DAOMXO010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41474_length_1709_cov_4.337969"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..717)
                     /locus_tag="PLH57_10215"
     CDS             complement(184..717)
                     /locus_tag="PLH57_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03027.1"
                     /translation="MSDDKKKSGFANTHYPGITADPNEGQFDIPKEALDFLKPQIDKF
                     GLDAQRAIWRRAVLLVEFNSKAAAVIEQLVQSGVARVGVLTTNASPQERDEIKKLSSD
                     LRSKYSWLEFEPFIGLSLAREPFDVLKGFQLLIDFSEGQLNSKVQETAIELKMKVLKG
                     SELPEKKVEHLVALLVG"
     gene            800..1444
                     /locus_tag="PLH57_10220"
     CDS             800..1444
                     /locus_tag="PLH57_10220"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRK03028.1"
                     /translation="MKKPILQSIEFYEMYVETSTGKMQTYTGAVAFGARRAEGCEVLK
                     ILTPTPDLRVLHEQLGNLHHARLRERLIFLQMRETQVTDVLQSFCQQGLPLPNVYDDS
                     IARLEGFSVFQLACYEQTCRIPHGETRAYAWLASRIKKYGAERAVGGAMRSNPFPLII
                     PCHRVVKKDGNLGGYMGTTTPASWQLGLKKTLLEVESLHRQPSLFALPNLVAIA"
     gene            1473..>1709
                     /locus_tag="PLH57_10225"
     CDS             1473..>1709
                     /locus_tag="PLH57_10225"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRK03029.1"
                     /translation="MQPFEWKFPTHVVFGAGRFQSVHKFVKGMGRRVLIVTSKSFALG
                     GPRDFLLRNLTEQLTKSGIELQTFSKIEANPRTDT"
BASE COUNT          461 a          413 c          383 g          452 t
ORIGIN      
        1 gacaaaaaat tgtccgggca agatttgatc gtgttgtggt tttttaaata ctccatttcg
       61 gaatgcattc ctcgactaat ccattcgcta taaaaattaa gagcggttcc atatgctgag
      121 ttggatgcgt cgacacaacc gaccatgtcg aagccaattt caaatgctgt tttttttata
      181 aattcagccg acgaggagtg cgacaagatg ttcaactttt ttctccggta attcggagcc
      241 cttcaaaact ttcatcttta attcgatcgc tgtttcttga accttcgaat tgagctgacc
      301 ttccgaaaaa tcaattaaaa gttggaaccc cttgagcacg tcgaatggtt cccgagcgag
      361 cgacaggcct ataaatggct caaactctaa ccatgagtat ttagatcgca gatcagagga
      421 gagtttttta atttcgtcac gttcctgggg tgatgcattg gtagttaaca ctccgacccg
      481 cgccaccccc gattgaacca attgctcgat aaccgcagca gctttcgaat taaattcgac
      541 caacaatact gcccgtctcc atattgcgcg ctgcgcatca agaccgaact tatctatttg
      601 gggctttaga aaatctaacg cttctttagg aatgtcgaat tgaccctcgt tggggtcggc
      661 agtgattccg ggataatgcg tgttggcaaa gcccgatttt tttttatcgt cgctcaattt
      721 agcaccctac aaaaactcta aactcaaaaa tcggaattga cgaggtagaa actttagaaa
      781 atgctgggaa cccattctta tgaaaaaacc aattctgcag tcgatcgaat tttacgaaat
      841 gtacgtcgaa actagtaccg gaaaaatgca aacctacaca ggtgccgtcg cattcggtgc
      901 acgacgagca gaagggtgtg aagttctcaa gatcctcaca ccgaccccag acttacgcgt
      961 tttacacgaa caacttggca acctgcacca cgccagactt cgcgaacgtc tgattttttt
     1021 gcagatgcgt gaaacccaag tcaccgacgt attacagagc ttttgccaac aggggttgcc
     1081 gttaccgaac gtgtacgacg acagcattgc tcggctcgaa gggttttctg tttttcaatt
     1141 ggcttgttac gagcaaacct gccgtattcc ccacggagaa actcgggcgt acgcgtggct
     1201 cgcgtcgcgg attaaaaaat acggtgccga acgggcggtt ggcggggcga tgcgttccaa
     1261 cccgtttccc ctcatcattc cctgtcaccg ggtggttaag aaagacggta atctcggtgg
     1321 ttacatggga accactaccc cggcaagctg gcagctcggt ctaaaaaaga cgttattaga
     1381 agtcgaaagc ttgcatcgtc aacctagttt gtttgcactg ccaaatctgg tagctattgc
     1441 ctgaccacca attattgcta acgaggtgac tcatgcagcc attcgaatgg aagtttccga
     1501 cccacgtggt attcggtgcc ggacgttttc aatcagtgca taaatttgta aaagggatgg
     1561 ggcgccgagt tttaattgta acgagcaaat cctttgctct cggcggacct cgcgatttct
     1621 tactgcgaaa tttgaccgaa caactaacta aatcaggaat tgaacttcag accttttcga
     1681 aaatcgaagc aaatcctcga accgatacg
//
LOCUS       DAOMXO010000552         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_41495_length_1708_cov_5.169389, whole genome shotgun sequence.
ACCESSION   DAOMXO010000552 DAOMXO010000000
VERSION     DAOMXO010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41495_length_1708_cov_5.169389"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLH57_10230"
     CDS             complement(<1..364)
                     /locus_tag="PLH57_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HRK03030.1"
                     /translation="MNKKLSVCVAALSLVLTWSFVGCGGPKAFTKGEYDDPTRVELLD
                     DKFNEADMQQMADTVIRAMVACPYVAKAAKPPVVIVERVQNRTQEHIDMVSMTDMIRS
                     ALIKSGKVRFVNKEERGTI"
     gene            425..1315
                     /locus_tag="PLH57_10235"
     CDS             425..1315
                     /locus_tag="PLH57_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRK03031.1"
                     /translation="MFPSNWKTRKIIFVLGKGGVGKSTVSLQIAHFLAQGTKTILLCR
                     IGSVHDAQQVPRKIAPGITELSLNSKDCFHEYVKLKLPIKTLSGILLGSKITDYLQNA
                     APGIPELVMTGKVWFERENFDHVVVDMPSSGYALTMLQTPKNFGDLFPGGPIYQDCHA
                     MLETFSNKNETALVVVTLPEEMPVQESCEFADKVTNMLAPNRPYLVINRVIPSSAKLD
                     SLYEKLGRNQDARHPFVANLCHVRAVSHGHARSIAGIFADEKLTPRFAEAPVIVYDQD
                     FLPDSPDTPTEERSQTWGQA"
     gene            1300..>1708
                     /locus_tag="PLH57_10240"
     CDS             1300..>1708
                     /locus_tag="PLH57_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRK03032.1"
                     /translation="MGTSVRNETIDDLIRSKRVLILCGSGGVGKTTSSGALALRAAEL
                     GRRTVVVTIDPAKRLATSLGISSLSSTPSNLTHLIKGKTPSAGELWAVMPDTNEVFES
                     FIKSMAKTQAKAAERVLRTSIYKIFVQDFSGANE"
BASE COUNT          466 a          439 c          378 g          425 t
ORIGIN      
        1 cgatcgtgcc gcgttcttcc ttgttaacga aacgtacttt accggactta atgagggccg
       61 aacgaatcat gtcggtcatc gaaaccatgt caatgtgctc ttgagttcga ttttgaaccc
      121 tttcaactat cactaccggg ggcttggccg ccttcgcgac atagggacac gctaccatgg
      181 cgcgaataac agtatcggcc atttgctgca tgtcggcttc attgaatttg tcatccaaaa
      241 gctcaacccg ggtggggtcg tcgtattcac ccttcgtaaa cgccttgggg ccaccgcacc
      301 ctacgaacga ccaagttaaa accagcgaaa gcgctgcaac gcatactgac aatttcttat
      361 tcatgcccct atttcataat ggctcaccct agaattcaag gggaattcaa gatagtcttt
      421 tgccgtgttc ccttcaaatt ggaaaaccag aaaaatcata tttgtccttg gaaaaggcgg
      481 ggttggaaag tcgaccgttt cgctgcaaat tgcgcatttt ctcgcccaag gcaccaaaac
      541 catattgctt tgtcgcatcg gatcggttca cgacgctcaa caagtccctc gcaagattgc
      601 tccaggaatt actgaactta gcctaaattc caaagattgt tttcacgaat acgtaaagct
      661 gaagcttccg attaagacgc tctcgggcat tttactcgga agtaagatca ccgattattt
      721 gcaaaacgca gcccccggaa tacccgaact cgtcatgact ggaaaagttt ggttcgaacg
      781 agaaaatttt gatcatgtgg tcgtcgatat gccttcaagt ggctacgcac tcaccatgtt
      841 gcagactcct aaaaattttg gcgatctttt tccgggtggt ccgatatatc aagactgcca
      901 cgccatgctt gaaacctttt cgaataaaaa cgaaaccgcg ctggtcgtcg taactttgcc
      961 agaggaaatg ccagtgcaag agtcgtgtga atttgcagac aaagttacaa acatgctggc
     1021 accgaaccga ccatacttag tcattaaccg agtaatccct tcgtcagcaa aactcgattc
     1081 actttacgaa aaattaggcc gcaaccaaga tgcccgtcat ccgttcgtcg caaatctttg
     1141 tcatgtgcga gctgtttctc acggacacgc gcgctcaatc gcgggcatat tcgccgatga
     1201 aaagctaact cctcgattcg cagaggcgcc ggtcatcgtc tatgaccagg atttcttacc
     1261 agactccccc gatacgccga ccgaagaaag gagccaaacg tggggacaag cgtaagaaac
     1321 gagacaatcg atgatctaat ccgatctaaa cgagtcctta tactttgtgg atcaggcggg
     1381 gtcggtaaga ccacctcaag tggggcgctt gctctgcgtg ccgcagaatt aggaagaaga
     1441 actgtagttg tcaccattga cccagctaaa agactggcaa cttctctggg catctcgtca
     1501 ctttcatcaa cgccgtcgaa ccttactcac ttaattaaag gaaaaactcc atccgccggc
     1561 gaattgtggg ccgttatgcc cgacactaac gaagtatttg aaagttttat taagtccatg
     1621 gccaaaaccc aggccaaagc ggccgaaaga gtactgcgta catcgattta taaaatcttt
     1681 gttcaagatt tttcgggtgc caatgaat
//
LOCUS       DAOMXO010000553         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_41503_length_1708_cov_3.775560, whole genome shotgun sequence.
ACCESSION   DAOMXO010000553 DAOMXO010000000
VERSION     DAOMXO010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41503_length_1708_cov_3.775560"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLH57_10245"
     CDS             complement(<1..668)
                     /locus_tag="PLH57_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03033.1"
                     /translation="MDSDNKKKKPSFDDVLNRGASVDKIFEDAPIPKGTDPFEKLEPV
                     ATESVTISPLAVEEASADFEQIGGATPVNILDNDTLFCVNRKFAALEELFRLVGRDCT
                     FGELTSEILRIAMEHVKSEAGSIVEIDFENNNMFFRAVSGRSSKNLLTFTIPRGKGVV
                     GFVCENQQVMALSKVDESSVYLKTISDSVGFETRNIVAYPLVIRAVTFGCLELLNRLG
                     EDFYT"
     gene            complement(767..1219)
                     /locus_tag="PLH57_10250"
     CDS             complement(767..1219)
                     /locus_tag="PLH57_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03034.1"
                     /translation="MYRNFILVVSILVLGACSPKKSAESPENSLREYVSRTFAIDNVE
                     DKNRLIELTTGKVKAAITTMSDADFQRHFIDESKDFIGMKIKDERALSDGRFSITYEL
                     SFKNKTVNTEAKILTKKHAIFEKIDERWLISEVQSLKTVIEHQNEMSF"
     gene            complement(1209..>1708)
                     /locus_tag="PLH57_10255"
     CDS             complement(1209..>1708)
                     /locus_tag="PLH57_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03035.1"
                     /translation="LTAGFITFVVSSNIYFLLLTKNYALAFYALFVAISSIFFHISVY
                     KLLKAAYLNPGLRWFEGKPRFIPRLNAYLSFDGAAPFQVRFTKLANDGCFVYSVAEQF
                     RKNKGRGVLQLELEGVTTQSQVDLISVVDRGKGMGLRFVENSFDSNKEILDLIDSVRS
                     AGYVS"
BASE COUNT          471 a          406 c          338 g          493 t
ORIGIN      
        1 gtatagaaat cctctcccaa gcgattcaat aactcaaggc aaccaaacgt tacggctctt
       61 atgacaagcg ggtaggctac aatattacga gtttcgaaac ccaccgaatc gcttattgtt
      121 tttaagtata cactgctctc atcaaccttg gacaaggcca ttacttgttg gttttcgcat
      181 acgaaaccca ccacgccttt gccgcgcggg attgtaaaag ttaaaagatt cttcgatgat
      241 cgcccagata ctgctctaaa aaacatatta ttattttcga aatcgatttc gacgatagag
      301 cccgcttccg acttcacgtg ctccatggct attcgtaaaa tttcgctagt aagctcgcca
      361 aatgtgcagt cccgacctac tagccgaaac aattcctcaa gcgcggcgaa cttcctattt
      421 acgcaaaata aagtgtcgtt atctaagata ttaacgggtg ttgcaccgcc tatttgttcg
      481 aaatcagcgc tagcttcttc gacggcaagt gggcttattg tcaccgattc agtcgctact
      541 ggttctagtt tttcaaacgg gtcggtgccc ttagggatgg gcgcatcttc aaaaattttg
      601 tctaccgacg cgccacgatt taatacgtcg tcaaacgacg gttttttttt cttattatcc
      661 gagtccatac cgcagtaggt gctccctcta tataggcttc ggattaagga gcaaacctct
      721 taaacactgg cggatctagg gcgttcgggg tggcattttc tgcctactaa aagctcattt
      781 cgttctgatg ctcgataacg gttttcaaac tttggacctc tgaaatcagc caccgttcat
      841 cgattttttc aaatatggca tgtttcttag taagaatctt tgcttcagtg ttaacggtct
      901 tgtttttgaa agataactcg taggtaattg aaaaacgacc gtcgcttaac gctcgctcgt
      961 ccttgatttt cattccaata aaatctttac tttcgtctat aaaatgccgt tggaaatccg
     1021 cgtccgacat tgttgttatc gcggctttta ccttgccagt ggtcagttcg ataagtcggt
     1081 ttttgtcttc gacattgtcg atagcgaagg ttctagaaac atattctcgg agactgtttt
     1141 caggcgactc cgcgcttttt ttcggactgc acgcgccgag caccaaaata gaaaccacca
     1201 agatgaaatt acgatacata ccctgcgctc cttactgaat cgattaggtc caaaatctct
     1261 ttattcgaat caaaactgtt ctctacaaat ctaaggccca ttccttttcc gcgatctacc
     1321 accgatatta aatcgacctg ggactgagta gtcacgcctt cgagctctag ctgtaataca
     1381 cctcgacctt tattttttcg aaattgctct gccacagaat aaacgaaaca cccgtcgttg
     1441 gccagtttcg tgaacctaac ctgaaaagga gcggctccat cgaaagacaa atacgcattg
     1501 agacgtggaa taaaccgcgg ctttccttca aaccatctta gacccgggtt caagtaggcc
     1561 gctttcaata acttgtagac ggaaatatga aaaaaaatgg aactaatagc gacgaataag
     1621 gcgtaaaagg ccagtgcgta gtttttggtg agaagaagaa aataaatatt tgaactaact
     1681 acaaaagtaa taaaccccgc cgtcaata
//
LOCUS       DAOMXO010000554         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_41570_length_1706_cov_5.184737, whole genome shotgun sequence.
ACCESSION   DAOMXO010000554 DAOMXO010000000
VERSION     DAOMXO010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41570_length_1706_cov_5.184737"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLH57_10260"
     CDS             <1..209
                     /locus_tag="PLH57_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRK03036.1"
                     /translation="VFEKILEGKTKKLLDEICLVNQPFVKDPDKTITQLTSELVAKIG
                     ENVVIRRFSRYVLGEGIAKTSESA"
     gene            248..994
                     /gene="pyrH"
                     /locus_tag="PLH57_10265"
     CDS             248..994
                     /gene="pyrH"
                     /locus_tag="PLH57_10265"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRK03037.1"
                     /translation="MASSPESKQPKYKRILLKLSGEALAGNAGYGIDTDILESIAKDI
                     GAIRDMGVEIGVVLGGGNIFRGLAAASTGMDRAQADYMGMLATVMNSLALQDALERLG
                     VYTRVQSAIEIQELAEPYIRRRAVRHLEKKRVVIFAAGTGNPYFSTDTAASLRAMEIN
                     AEVILKATKVDGVYDADPVKHKDAKLFRELTYLDVLSKGLQVMDSTAISLCMDNRLPI
                     IVFNLNRPDSVRDVVLGKPIGTIVHSPEIK"
     gene            1009..1563
                     /gene="frr"
                     /locus_tag="PLH57_10270"
     CDS             1009..1563
                     /gene="frr"
                     /locus_tag="PLH57_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRK03038.1"
                     /translation="MKQVLDSLHEKMSKTINGFRGELVKVRTGRATPALLDGVKVDYY
                     GSDMPIAQVASVTCPEPRMIVVQPWEASMLQPIERAILIADLGLNPQNDGKIIRVPLP
                     MLTEERRKELVKFTKKLGEECKVALRNERRDANEEIKKLEKDKQLSIDEAKKSTEQIQ
                     KKTDDFVAEVDKLLATKEKEILSV"
     gene            1563..>1706
                     /locus_tag="PLH57_10275"
     CDS             1563..>1706
                     /locus_tag="PLH57_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017032656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HRK03039.1"
                     /translation="MSSGLPKHIAIIMDGNGRWAERRGRPRIYGHIKGCGRVREIIRE
                     ADRL"
BASE COUNT          504 a          372 c          448 g          382 t
ORIGIN      
        1 aagttttcga aaaaattctt gaagggaaga ctaaaaaact tctcgatgag atttgtctcg
       61 tgaaccaacc gttcgtgaag gatcctgata aaacgatcac gcagttaacg tcggaacttg
      121 tagcaaaaat cggcgaaaac gtagtgatac gtcgctttag tcggtacgtc ttaggcgagg
      181 gcatcgcaaa aacatctgaa agtgcttaac tgaattgtaa caccggcgaa aacgaggggc
      241 atttttcatg gcgtcatctc cagagagcaa acaaccgaag tataaacgta ttttattaaa
      301 gctttcgggc gaggcactgg cgggaaatgc tggctatggg atcgacaccg acattttaga
      361 gtctatcgca aaagacatcg gcgcaattcg cgacatggga gtcgaaattg gagtggtgtt
      421 aggtggagga aatatttttc gcgggttggc cgcagcctcc actggaatgg accgcgctca
      481 agccgactac atgggtatgc tcgccacagt gatgaattcc ttagcgctac aagatgctct
      541 tgagcgcttg ggcgtttaca ctcgtgttca gtcagccatc gaaattcaag agcttgccga
      601 gccctacatt cgaaggcgcg cagtacggca tttagaaaag aagcgggttg taattttcgc
      661 ggccggaacg gggaatccct atttcagcac ggacactgcg gctagcttga gggcgatgga
      721 aatcaacgct gaagtgattc taaaggccac caaagtagac ggcgtttatg atgcagatcc
      781 agtgaaacac aaggacgcaa agctgtttcg cgaattaacg tatctcgatg ttcttagtaa
      841 agggctgcag gttatggatt caacggccat ttccctttgc atggacaacc gactgccaat
      901 tattgttttt aatctcaacc gaccggatag cgtgcgtgac gtggttctag ggaagcccat
      961 tgggaccatc gtgcacagtc cggaaatcaa atagaggtcg acaaaaccat gaaacaagtc
     1021 ctcgatagtt tgcacgaaaa aatgtcaaaa accatcaatg gttttcgcgg agaactcgtc
     1081 aaggtacgaa ctggtcgggc gacccctgcc ttgctcgatg gagtaaaggt cgattactac
     1141 ggatcagata tgccaatcgc gcaggttgct tcggtgacct gcccagagcc acgtatgata
     1201 gtggttcagc cctgggaagc ttcgatgctc caaccaatcg aaagagcaat tttgattgcg
     1261 gatctcggat tgaatcctca gaacgacggt aaaattattc gcgttccgct tccgatgctc
     1321 accgaagaac gccgcaaaga gctagtgaag ttcactaaga aacttggcga agagtgcaaa
     1381 gtagcgttga gaaacgagcg tcgagacgca aatgaagaaa taaaaaaact agaaaaagac
     1441 aaacaactct caattgacga ggccaaaaaa tcgactgagc aaattcagaa gaaaaccgat
     1501 gactttgtgg ctgaagtcga caaactcctg gcgactaaag agaaagaaat tttgagcgtc
     1561 taatgagttc tgggctaccc aaacacatcg cgatcatcat ggatgggaat ggtcgttggg
     1621 ctgaacgcag aggtcggcct agaatttatg gccacatcaa agggtgcgga cgggtacgcg
     1681 agatcattcg tgaagccgac cgtttg
//
LOCUS       DAOMXO010000555         1705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_41616_length_1705_cov_3.811515, whole genome shotgun sequence.
ACCESSION   DAOMXO010000555 DAOMXO010000000
VERSION     DAOMXO010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41616_length_1705_cov_3.811515"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="PLH57_10280"
     CDS             <1..1212
                     /locus_tag="PLH57_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03040.1"
                     /translation="CSSKKIRSSIVKYLTLLAGLFYAFNTSAKSLNDYTYYFHPTSTL
                     TLGKGLDPAQPNEEKRICFDFPEPNKKTEGALKTTASIEVLENTRNITAILNYDTNID
                     ANLLFFSANTQFDFDNTYTLDKRSVSVVVKASTEYGRLFLKKPVLLPEYAEMLDKKQF
                     NQFRTFCGSRLVTTEVRGATVAVVITIENASSELISKLKITGSGGKSVAGIGAKAKAM
                     FEAEYRDKSTNNEVRAQLFAVGGNGISDLDKVVQNMVRRGERLDEIADGLQKFISGFT
                     VDNSAPVRFYTTHIDPRLDDRYSDLIGEKKTKALLDIVWAYRNAELMNGVVTKFLNNI
                     NDERRLNFKKENLISASEELPLLTDHIEELQNRHAECLLANNSDVKKRCKMPSAPDLF
                     WTEVIYDIIGE"
     gene            1364..>1705
                     /locus_tag="PLH57_10285"
     CDS             1364..>1705
                     /locus_tag="PLH57_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03041.1"
                     /translation="MCFSANASFLASAGIGAVGAATLLKKPTIRELPLALIPVCFSVQ
                     QASEGIVWLTLPTGGLLHSVAVKVFLLFATMLWPVLVPIAFYLVEQDSSRRKKMRFGI
                     GLGLAVVAYLLY"
BASE COUNT          511 a          381 c          377 g          436 t
ORIGIN      
        1 tgttcaagta aaaaaattag gagttcaatc gtgaaatatt taacactgct tgctggattg
       61 ttctatgcct tcaacacaag tgcgaagtcg cttaacgact atacgtacta ctttcatccg
      121 acgagcactc ttactcttgg aaaaggacta gatccggcgc agccaaacga agaaaagcga
      181 atatgttttg acttccctga accgaacaaa aaaacagagg gcgcgctaaa aacgacagcc
      241 tcaattgaag tacttgaaaa tactcgtaat attacggcaa tcttaaacta cgatacaaat
      301 atagatgcaa acctcttatt tttttcggca aatacccagt tcgacttcga caacacatat
      361 accctagata aacgcagcgt ttccgtggtc gtgaaagcaa gtacggaata cggtagacta
      421 tttttaaaaa agcctgttct attgcctgaa tacgccgaaa tgttggacaa gaaacagttt
      481 aaccaattca gaacgttctg tggttcccgc ctcgtgacca ccgaagtccg cggcgctacc
      541 gtagcagttg ttattaccat tgagaatgca agtagcgaac tcatttcgaa actaaaaatt
      601 accggaagcg gcggaaaaag tgtggcgggt atcggtgcga aggcaaaagc tatgttcgaa
      661 gcagaatatc gcgacaaatc aacaaacaat gaagttcgcg cacagctttt cgcagtgggc
      721 ggcaatggga tttccgatct agataaagta gttcaaaata tggttcgccg tggtgaacga
      781 ctagacgaaa tcgctgatgg attgcaaaaa tttatttcag gtttcaccgt agataactca
      841 gcacccgtac gattttatac gacccatatc gacccgaggc tcgacgatcg ctactctgac
      901 ctcatcggcg aaaaaaaaac taaggctcta ctcgacattg tgtgggccta cagaaatgct
      961 gaactcatga atggagtggt tacgaaattt ttgaataata taaacgacga gcgccgtcta
     1021 aattttaaga aagaaaatct catcagcgcc agcgaagagt tacctctgct tacagaccac
     1081 atcgaagagc ttcaaaatag acacgccgag tgcttgttag caaataattc cgatgtgaaa
     1141 aagcgttgca aaatgcccag tgcgccggat ttattttgga cagaagtcat ttacgatatt
     1201 atcggcgagt agccgccgcg cttttggcgg gttcttttga aacttctgta aatacctgga
     1261 ccttcagcgt cgaccacagg atcccgaata cctttagcgc ctaacgccaa actctaaagt
     1321 aattccaaac tttagccgag attacctaga gaaagaggaa gctatgtgtt ttagcgcaaa
     1381 tgcgagtttt ctagcgagtg ctggtattgg cgcggttggt gctgcgacgt tgctaaaaaa
     1441 accaacaatc cgcgaactac ctctcgcgct tattcccgtc tgtttttcag ttcagcaggc
     1501 atcagaaggc atcgtctggc tgactttgcc aacgggcggc cttctacact ccgtggcagt
     1561 aaaagtattt ctattatttg cgaccatgct ctggccggtt ttggtgccga tcgcttttta
     1621 tctagtcgaa caagatagtt cacgaagaaa aaagatgcga tttggaatag gcctcgggct
     1681 agccgtcgtg gcctatttac tttat
//
LOCUS       DAOMXO010000556         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_41695_length_1703_cov_3.844660, whole genome shotgun sequence.
ACCESSION   DAOMXO010000556 DAOMXO010000000
VERSION     DAOMXO010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41695_length_1703_cov_3.844660"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLH57_10290"
     CDS             complement(<1..327)
                     /locus_tag="PLH57_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03042.1"
                     /translation="MFRKKSTTATLTTALVAALVMLALPRTAMTAEKKSPAKTKEDVA
                     AQPAEGEVDISDVQSQYWKAHDKQFEVIQNKLYTKAERFELSAMFGLYQRVDFQDAKT
                     LGGSLAY"
     gene            complement(358..>1703)
                     /locus_tag="PLH57_10295"
     CDS             complement(358..>1703)
                     /locus_tag="PLH57_10295"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HRK03043.1"
                     /translation="IGSRKVRLVRKLISSDNLLKSAWEPITDSKDKTLTLRVRIYEKN
                     IPLEIKNSTNEFWIEDQYDVVGAHRLAWKQGGKVLAQIPASWVSGGGRVQIGKKAIAK
                     LEDWTSAQELETDTVYRIYHGRFIVEILASEGQYRIGAGRVDVLPKDLRKPLFVSSFV
                     TMVIFFAWMLLVGKPEAEVEEPYSLYARIQNVPIEKIPDAPSEQQQAGGGSGAQNAEA
                     SAPTQGSASSAPSQLAASLTGGLKSLMGGIISQSKVASNAVVSETGIGQATAVATAGA
                     VQTGKLAAVGNGASNVAGAAKLGALALAGSGKGYGGGTGTGLGTGVGSGVGSGVGNGI
                     GGGGFRLIEEESIVDGGLDKSVIAAVIQNNLSQIKYCYERQLVADPDLFGKVVVKWEI
                     TAKGLVEGALVKQTTLASAPVENCMLSKISGWKFPQPKNGSKVTVTYPFLFKSTR"
BASE COUNT          367 a          445 c          416 g          475 t
ORIGIN      
        1 gtaggctagt gagccgccca gggttttggc gtcttgaaaa tccactcgtt gataaagacc
       61 gaacatggca cttaactcaa aacgttcggc cttggtgtag agtttattct gaataacttc
      121 gaattgtttg tcgtgtgcct tccagtattg gctttgaacg tcggaaatat cgacctcacc
      181 ttcggcgggc tgagcggcaa catcttcttt ggtttttgcg ggcgactttt tttccgcagt
      241 catcgctgtg cgaggcagtg ctagcataac gagtgcggca acgagcgcgg tggttagggt
      301 tgctgtggtt gatttttttc taaacatggt gagttcccct tttatggttc gtcaattcta
      361 ccgcgttgat ttaaacaaga atggataagt gactgtcacc ttcgaaccat tcttgggttg
      421 tgggaacttc cagcccgaaa tctttgagag catgcaattt tcgaccggtg ccgaggccaa
      481 tgtagtttgc ttcacaagcg caccttccac taaacctttg gcggtaattt cccacttcac
      541 tacaacttta ccaaaaagat cagggtccgc cacaagttgt cgctcgtaac agtattttat
      601 ttgtgaaaga ttgttttgaa tcactgcagc aattacgctc ttatctagcc caccgtcgac
      661 gatgctttct tcttcgatta agcgaaaacc accgccacca attccattgc ccactccgga
      721 gcctacgcct gaacccactc cggtacctaa acctgttcca gtgcctccac cgtatccttt
      781 tcctgaaccc gcaagagcta gcgcgccgag tttagcagcg cccgcaacat tcgaggcgcc
      841 attgccaacg gcagccagtt tgccagtttg caccgcgcct gcggttgcca ctgcggtggc
      901 ttgtccgatt ccggtttccg ataccactgc gttgctagct actttcgact gcgaaataat
      961 tccgcccatt aagcttttga gtccacctgt caaactcgcg gcaagctgcg atggggcgct
     1021 cgaggcactg ccttgggttg gcgcgcttgc ttcagcgttt tgtgcgccgg aaccgccgcc
     1081 agcttgttgt tgttccgagg gggcgtctgg gattttctcg attggcacat tctggattcg
     1141 tgcgtaaaga gagtagggct cttcgacttc ggcctcaggt tttccgacta ggagcatcca
     1201 ggcgaaaaag atcaccatcg ttacgaaact tgaaacgaag agcggtttgc gaagatcttt
     1261 aggaagtaca tcgacgcgtc cggcgccaat ccggtattgg ccctccgagg caagtatttc
     1321 tacaatgaag cgcccgtggt agattcgata aacggtgtcg gtttcaagct cctgagcgct
     1381 ggtccaatct tcaagtttgg cgatcgcttt tttcccaatt tgaacgcggc cgccgcctga
     1441 aacccaactg gcagggattt gagctaaaac tttgccgcct tgtttccagg ctagccgatg
     1501 tgcgccaaca acgtcgtact gatcttcgat ccaaaactcg ttggtactgt ttttaatttc
     1561 cagcggaata tttttttcgt aaattcggac acgaagcgta agagttttgt ccttggaatc
     1621 agtaatgggt tcccacgcgc ttttcagtaa gttatcgctc gatatcaatt tacgaaccaa
     1681 gcggaccttc cgtgagccaa ttt
//
LOCUS       DAOMXO010000557         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_41899_length_1698_cov_4.925137, whole genome shotgun sequence.
ACCESSION   DAOMXO010000557 DAOMXO010000000
VERSION     DAOMXO010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41899_length_1698_cov_4.925137"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLH57_10300"
     CDS             complement(<1..638)
                     /locus_tag="PLH57_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK03044.1"
                     /translation="MSAAKVQTGDVVRWWGPLSVALGAGLWGLESLFRVRLGEHFPAD
                     LLVFYEHVMGTVVLAPVLWFARKQVAQLTKKAVGWLLISGVVGSAFGTVLFTASLKHL
                     NVSVATVLLNLQPLVAISMASILLKERMAKGFAWWAALAVAAGVVITFEHVENFRVEN
                     PVGILCVFGAILCWGTATVAGRGLMRETSIWVAAPLRFAVGMLATLGVVVFGG"
     gene            complement(642..>1698)
                     /locus_tag="PLH57_10305"
     CDS             complement(642..>1698)
                     /locus_tag="PLH57_10305"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03045.1"
                     /translation="EISLKLAKNAQYFEEKMPYGEFRKKFPPEYSPPALVAYYLQEIS
                     SMRTAGYNLPNYDDIRRDVGFKNIIRQDPLSLEQDPIVRDERRQMYQEFLVASKVDEV
                     LELGPRAKKLKTLLHEVIGHGSGTYDVTKYGDKEDPVSAMGSPASSLEEQRADLTALV
                     FGADAKLVEVGIFKDQAEADQILRVLYDDYMADFLRNLAKNRSFAQSHHRGRWLFINK
                     LLEAGAIEWAAKDGEQISDRNRVLNVKDYALFHATALKTLEDLQRIKASRGETEMREL
                     FAKYAPLDAINEPWAQAIIERGAKLRMSNGTVEQPWKMNRKGVVKVFGDSSSMESIAT
                     FWNACLYKPHLCREASE"
BASE COUNT          455 a          470 c          361 g          412 t
ORIGIN      
        1 ccaccaaaaa caacaacacc gagggtagcc aacatcccaa cggcaaaccg caacggtgcg
       61 gccacccaaa ttgaggtttc gcgcataagg ccacgacccg ctactgtggc ggtaccccaa
      121 cacaaaatag caccgaacac acaaagtatt ccgacgggat tttcgactct aaaattttcg
      181 acatgttcaa acgttatcac cacacccgcg gccactgcca aggcagccca ccacgcgaat
      241 cccttggcca ttctttcctt taaaagaatc gaagccattg aaatcgcaac gagtggttgt
      301 aagtttagca gtacggtggc gacggaaaca ttgagatgtt taagcgaagc agtgaataac
      361 accgttccaa aagcagagcc gacaactccc gaaatgagca accaacctac agcctttttg
      421 gtaagctggg cgacctgttt gcgagcgaac cacagtactg gcgctaaaac gacggtgccc
      481 atgacgtgtt cgtaaaacac caatagatcg gccggaaaat gttcgcccag tcgtacccga
      541 aataacgact caagacccca caacccggcg cctagtgcga cactgagcgg gccccaccaa
      601 cgaacgacgt cacccgtctg aaccttagcg gcactcatcg attactccga tgcctcccga
      661 cataaatgtg gtttatacaa acaggcgttc caaaaagtag cgattgattc cattgaactg
      721 gaatctccga aaacctttac aacgcccttt cgattcattt tccatggttg ctcgacagtg
      781 ccattactca ttcgtagttt tgcccctcgc tcaataatcg cttgagccca gggttcatta
      841 atcgcatcga gcggagcgta tttcgcaaaa agttcccgca tttcagtttc accacgactt
      901 gctttaatcc gctgaaggtc ttccaaagtt tttagagcag tcgcgtggaa taaagcatag
      961 tctttaacat tcaaaacgcg attccgatcg gagatttgct ctccatcctt tgcagcccat
     1021 tcaatggctc ctgcctccag cagtttattt atgaaaagcc atcggccacg gtgatgcgac
     1081 tgcgcaaacg aacgattttt agccaggttt cgcaagaagt cggccatgta gtcatcgtac
     1141 aacactcgca aaatttggtc ggcttcggct tggtctttaa aaattccgac ttcaactaac
     1201 ttcgcatctg ctccgaatac caacgccgta agatcggccc gttgttcttc caacgaactt
     1261 gcgggagaac ccatggccga aaccgggtct tctttatctc cgtacttcgt aacatcgtat
     1321 gtgccagagc catgaccgat cacttcatgc aacagagttt tcagtttctt cgcgcgtgga
     1381 ccgagttcca gaacctcgtc gactttagaa gcaacaagga actcctgata catttgtcgg
     1441 cgctcgtcac gaacaattgg atcttgctct aaactcagtg ggtcttgtcg aatgatattc
     1501 ttaaaaccta cgtcgcggcg aatatcatcg taattcggca aattgtatcc tgccgttcgc
     1561 atcgaagaaa tttcttgtag gtaatacgcg accagagccg gcggcgaata ctccggagga
     1621 aatttttttc gaaattcccc atacggcatt ttttcttcga agtactgggc gtttttcgct
     1681 agttttagcg aaatctcc
//
LOCUS       DAOMXO010000558         1696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_41961_length_1696_cov_4.470445, whole genome shotgun sequence.
ACCESSION   DAOMXO010000558 DAOMXO010000000
VERSION     DAOMXO010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41961_length_1696_cov_4.470445"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLH57_10310"
     CDS             <1..258
                     /locus_tag="PLH57_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HRK03046.1"
                     /translation="YDKIWQAFAVHLPVQSVGVMGDGRTYDNVVALRAVTSADGMTAD
                     WFRFDSEFLERVSNRITGEVRGVGRVVYDLTSKPPATIEWE"
     gene            262..1398
                     /locus_tag="PLH57_10315"
     CDS             262..1398
                     /locus_tag="PLH57_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRK03047.1"
                     /translation="MAFVHLHFHSQYSLLESAVKIPDALARAKELAMPALALTDLGNM
                     FGAVDFYLAAKSAGIKPILGCEIYYTSGSRLTKNAVSKKRINVSADEEEANRLYHLVL
                     LAKDADGYRSLCELVTRTYTEGFHHGKPRADRELIEKYSKGLVCLSAGIKGEVGYHCA
                     TGRPDRATETARWFQSVFKDDYFLELQENGLPPQSNANSMCVDIGKELGVGLVACADV
                     HYLKKELAPAHEVLMCIGAGRNVDDSRAVRLNPDEFWFKDEATMRSQFHWMPEAVDNA
                     ALIAEKCNFQFKLKDEKGNQIYHLPAYLPSQWEDTNSERPPHMQIQSDLAETHRAEVF
                     SADGTFSSENFLRLLSRKGLEDRFAVIAARRASSGPLTEEIKKT"
     gene            1405..1696
                     /locus_tag="PLH57_10320"
                     /pseudo
     CDS             1405..1696
                     /locus_tag="PLH57_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          402 a          380 c          470 g          444 t
ORIGIN      
        1 tacgataaaa tttggcaagc tttcgcggtt catttgccgg tgcaaagcgt aggggtcatg
       61 ggagatgggc gcacttacga caatgttgtc gcgctacgag ccgttacttc ggcagacggt
      121 atgaccgctg attggttccg ctttgatagc gagtttcttg agcgagtgtc gaatcgtatt
      181 actggtgaag tgcgtggcgt aggacgtgtg gtttatgatc ttacgtcgaa acctcccgcg
      241 acgatcgagt gggaataacg catggctttt gttcacctgc attttcattc acagtatagc
      301 ttactagaga gtgcagttaa aattcccgac gcactagcac gcgccaaaga gctggcaatg
      361 ccggcgctcg cgttgactga tcttggcaac atgtttggtg cggtggattt ttatcttgcc
      421 gctaagagtg cgggaattaa accaatcctt gggtgtgaga tttattatac ctctggtagc
      481 cgattgacga aaaatgcagt ttccaaaaag cgcataaatg tttcggccga tgaagaggaa
      541 gccaaccgcc tttatcactt ggtgctgctg gcgaaagacg ccgacggata tcgcagtttg
      601 tgtgaactgg tgactcgcac ctacacagag gggtttcacc acggaaaacc acgggcagat
      661 cgcgaattga tcgaaaaata ttccaagggc ctggtgtgtc ttagcgcggg aattaagggt
      721 gaagtgggtt atcactgtgc gacggggcgg cccgatcgtg ccacggaaac tgcgcgttgg
      781 tttcagagcg tatttaaaga cgactatttt ttagagcttc aagaaaatgg ccttcctccg
      841 caatctaacg caaattccat gtgcgtcgat attggcaagg aacttggcgt agggctcgtg
      901 gcctgcgcgg atgtgcatta cttaaaaaaa gaactcgcac ctgctcacga agtacttatg
      961 tgcattggtg ctggccgcaa tgttgatgat tcgcgggccg ttcgcttgaa tcccgatgag
     1021 ttttggttca aagacgaagc cactatgcga agccagtttc actggatgcc cgaggccgtt
     1081 gataatgctg ctttgatcgc cgagaagtgc aatttccagt tcaaactgaa agacgaaaag
     1141 ggcaatcaga tctaccatct ccccgcgtat ttgccgtcgc agtgggaaga tacaaactcg
     1201 gaacgcccgc cgcacatgca aattcagagc gatttagcag aaactcatcg cgctgaagtt
     1261 ttttcggcag atggcacttt ttcatcggaa aattttcttc gactattgtc tcgtaagggc
     1321 ttggaagatc gctttgcggt gatcgctgcc cggcgtgctt cgagtggtcc gcttaccgag
     1381 gaaattaaga aaacctagta cgctcggctc gaagaagaag tcgaaatgat tattcgcacg
     1441 ggatttgccg ggtacttttt ggtcgttgcc gactttattg cttacgctaa atcgcacggg
     1501 attccggtgg ggccggggcg aggttctggc gcaggttcgc tagtcgctta tagtttacag
     1561 atcaccgatg tggacccgat accgtttaat ttgctctttg aacgattcat caacccggaa
     1621 cgcgttagta tgccggattt tgacgtcgat ttttgccagg agcgaagagg cgaagttatt
     1681 gattacgtcg tacgca
//
LOCUS       DAOMXO010000559         1693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42084_length_1693_cov_4.254579, whole genome shotgun sequence.
ACCESSION   DAOMXO010000559 DAOMXO010000000
VERSION     DAOMXO010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42084_length_1693_cov_4.254579"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1007..1498
                     /locus_tag="PLH57_10325"
                     /pseudo
     CDS             1007..1498
                     /locus_tag="PLH57_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
BASE COUNT          392 a          403 c          459 g          439 t
ORIGIN      
        1 ctttggaccg accgagcttg aggctcgccg caacgtgatg aacgagtgca aaaaacacaa
       61 tcacgccatg cattgtgacg aggtggagtg tcagcgaaac cagtagatgc atagcgtcaa
      121 aatatgctaa gtgggcgcga tgaaaaaaat cgtagtgtcc ttgagttttg gaatagccct
      181 tttgatgggc gccacgagtc actcaatagc agccccgaac gatctgcgtc cgatggccaa
      241 acaggtcagt gcggcgctaa cgtctcagac tcagcgtaat ctttcgattt ctccattcgt
      301 gcgcgaaggg cgcaaactcg gaaaacaagc tctcgatgcc ggatgtaacg ttgttcttgg
      361 cctgttagat ccaaggcgat ggcccgaatc tgcggagcga attggaaagg cgattgccta
      421 tgttcccgcg ctttcttttt atgctgttgc atcgacttcc gctaaattgt ttgacctgtt
      481 ttcgccttcg cggtcccgcg aagtctccta ccgagatcag gatttggagt tggagttggc
      541 cgtcgaggcc ccccctacga cccatctaga gaataccgtc caagaactct tagccgaaat
      601 tcgtttggtg ttggagtcca tgttattaag cgcgtccgat caatatttgg tggtcgcaat
      661 ttcggaaaat caggtgaagt ttgttgtcga tgggcagctc aatgtttttt catcggatag
      721 tttcgcccag cgcatgtttg aacgcgaaga cctgtttggg tcggtcgccc aagcaaagga
      781 agtgtgggag cagttcgtca aggctctacg cgttccagat ttacgcgttc gctacgactt
      841 gggtgtgttg gaaggattca accgagacct aattctttac tcaaaagacg tttcatctgc
      901 cgaaaaaatg gcgtcagtac ttaaaatagt gtcagcatcc cacgctgatt tcagtggcgt
      961 cgaggttccc agtgttcgac cgaattcgga ttggtaccaa acctcggtcg aacattatga
     1021 cgacgaatcg gtcgtacatt tttattctct caatcagccc tatggggagt tttccaattt
     1081 tgctcgctat cccatcgtgt tggaggggaa agtgtggcca actacggaac attattttca
     1141 ggccaaaaag tttgccggca ctgcgtacga ggaacgggta cggcacgccg cgggtccccg
     1201 cgaggctgcg gaccttggac gtgatcgcac gttgccgctc cgaagtgatt gggaacaggt
     1261 aaaagatcaa gtcatgctaa ccgcgttgcg tgcgaagttt tcgcagcatg actatttaag
     1321 tgatctatta ctcagtacta gtgaagcaag tcttgtggaa cacacgagcc acgatagcta
     1381 ctggggtgat gggggcgacg gcagcggccg gaacatgctg ggaaaactgt tggttcaggt
     1441 tcgaaaagaa ctgcgggaga ataaattccc cgctttttgg acgtccccgt atcgttaagc
     1501 agttccgcaa cttgatcttt tcaatgcgac gcgctaaaag cccgctttat ggcaaaattc
     1561 tggcccctgc tttttatttt cgcaacgcct ttggcgtttg gagttgtgga aaagaagccg
     1621 gagcgagggt gcactagtct gcttttgcag gcgatggaag ttccgtgcaa tcaccctgct
     1681 gcgtcttgtt ttc
//
LOCUS       DAOMXO010000560         1690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42202_length_1690_cov_3.354128, whole genome shotgun sequence.
ACCESSION   DAOMXO010000560 DAOMXO010000000
VERSION     DAOMXO010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42202_length_1690_cov_3.354128"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1690)
                     /gene="dnaK"
                     /locus_tag="PLH57_10330"
     CDS             complement(<1..>1690)
                     /gene="dnaK"
                     /locus_tag="PLH57_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRK03048.1"
                     /translation="VQEFAKVAPFKIADSKNGDAWIKVNDREMAPQEISALVLQKMKK
                     TAEDYLGEEVKEAVVTVPAYFNDAQRQATKDAGRIAGLNVLRIINEPTAAALAYGLDK
                     KKDEVVAVFDLGGGTFDISILEIGDGVFEVKATNGDTFLGGANFDQRIIDFLAESFQK
                     ENGIDLRKDRMALQRLKEAAEKAKHELSSSLETEVNLPFITADQSGPKHLSVKISRSK
                     LEALVADLIDKMVAPCETCIKDSGIEKSKINEVILVGGMTRMPRVQQKVKEIFGKEPH
                     RGVNPDEVVAVGAAVQGAVLKGEVKDVLLLDVTPLSLGIETLGGVSTKLIEKNTTIPS
                     RKSQVFSTAADNQTAVTIHVLQGEREFAEHNKSLGRFDLVGIAPAPRGMPQVEVTFDI
                     DANGIVNVSAKDLGTGKEQAIKITAGSGLTEAEIQRMVKEAEANRGEDEKRKAVVTAR
                     NNLDGLIYSIDKSLKEHGEKVDAPIKAEIETALTEARKALESNDEATLKSATETLTTA
                     SNKMAEVIYKASAANGAGAQAAAGEGNGSTNSDSTAKKKDGKDGDDVIDADFKEV"
BASE COUNT          372 a          446 c          368 g          504 t
ORIGIN      
        1 acttctttaa agtcggcgtc gattacatcg tcgccatctt tgccgtcttt ctttttagca
       61 gtcgaatcgg agttcgtcga tccgtttcct tcaccagcag ctgcttgggc gccagcgcca
      121 ttcgcagccg aagctttata aatgacttct gccattttat tcgaagccgt agttaaggtc
      181 tcagtggccg acttcaacgt ggcctcgtcg ttcgattcga gtgcttttct tgcttcggtc
      241 aacgccgttt caatttcggc tttgatcgga gcatctactt tttccccatg ctccttcaaa
      301 ctcttgtcga tcgagtaaat taagccgtct aagttgttgc gtgccgtgac tactgcctta
      361 cgtttctcgt cttcaccgcg attggcctca gcctctttga ccatgcgttg gatttctgct
      421 tcagtcaggc ccgagcccgc agtaatctta atcgcttgtt ccttaccggt tccgaggtct
      481 tttgcggaaa cgtttacaat tccgttcgcg tcgatgtcga aggtcacttc tacttgcggc
      541 attccacgtg gagctggggc aattcctact aagtcaaagc gcccgaggct cttgttgtgt
      601 tcggcaaatt ctcgttcccc ttgaagaacg tggatcgtta cggccgtttg attatcggct
      661 gcagtcgaaa agacctgact ttttctcgaa ggaatcgtgg tgtttttctc aataagcttg
      721 gtggaaacac cgcctaaagt ctcaattccg agcgagagag gagttacgtc taacaacaat
      781 acgtctttga cctcaccctt aagaaccgca ccctgtacgg cggcgccaac ggcaaccact
      841 tcgtcggggt tcacgccacg gtgaggttcc ttgccaaaga tttccttaac tttctgttga
      901 acgcgcggca tgcgggtcat accaccaacc agaattactt cgttgatctt cgacttttca
      961 attcccgagt ccttaatgca cgtctcacat ggagcgacca ttttatcgat caagtccgca
     1021 acaagtgcct caagtttcga gcgcgaaatt ttaacactca aatgttttgg ccccgactga
     1081 tcggccgtaa taaaaggaag gttaacctct gtctcaagag agctagaaag ctcgtgtttg
     1141 gctttttcag ctgcttcctt tagacgttgc agggccatgc ggtctttgcg caaatcaatg
     1201 ccattttctt tctgaaaact ttcagcaaga aaatcgatga ttcgttggtc gaaatttgca
     1261 ccgccaagaa aagtatcacc attggtggct tttacttcaa atactccgtc accaatttcc
     1321 aagatcgaaa tatcgaacgt accaccacca aggtcgaaaa ccgccacgac ttcgtctttt
     1381 ttcttatcta atccgtaggc cagcgctgcg gccgtcggtt cattgataat acgaagcaca
     1441 ttgaggccag caattcggcc cgcgtctttg gtggcttgtc gttgtgcatc gttaaagtaa
     1501 gctggtacgg tgactacggc ttctttaact tcttcaccca aataatcttc cgcggtcttc
     1561 ttcatttttt gaagcactag agcggaaatt tcttgaggag ccatttcgcg atcgttcact
     1621 ttaatccagg catcaccgtt tttcgaatca gcaatcttaa aaggggcaac tttcgcaaat
     1681 tcttgcacct
//
LOCUS       DAOMXO010000561         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42238_length_1689_cov_4.652999, whole genome shotgun sequence.
ACCESSION   DAOMXO010000561 DAOMXO010000000
VERSION     DAOMXO010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42238_length_1689_cov_4.652999"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1374
                     /locus_tag="PLH57_10335"
     CDS             157..1374
                     /locus_tag="PLH57_10335"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRK03049.1"
                     /translation="MMIRVWLVAAFVFSLFSNSLFSAEPLQPELRMLEMPAKNVAVVA
                     RVLGEKIQLIGEELVLVPADSVKAIGGILHKENGKCGGFIDVTDESMSHFEKSVAGLS
                     KFKTDESEWEEEQEPLPPLYEIDQTIVQVVKSVIPKNIENFANAYSKTFTTRYAASEE
                     GLEAPKWLAEVWTKMAHDANRSDILVELINPHKRYEQSSVRVTIPGTSSERAIVVIGA
                     HLDSINQGASDFAPGVDDDASGIATMTETFRVLLAHNFRPSATVQFFGYAAEEVGLIG
                     SRAIAESYRNYGAKVAGVMQLDMTGFPGRTNAVTFIRDHTSDELTTWTQAVFNMYVGL
                     AFVNDRCGYACSDHASWHRYKYRSVFPHEAEFNESNNRIHTDHDLWDEHMNAEHAGLF
                     ARLAVAFTLELAK"
BASE COUNT          437 a          420 c          423 g          409 t
ORIGIN      
        1 gagagcggaa ggcgattcct gtcgaaacta ccagttctac cattacgcgc gtcgcgcgat
       61 cgacacctta aaaaaaagat tttgcccccc tcttttctcg ctgctgatct ggcgttactg
      121 tcttcttaca aaagaatccc aatggagggg acgatcatga tgattcgcgt gtggctagta
      181 gcagcttttg tattttcttt gttttcaaat tcactttttt cagccgaacc ccttcaacca
      241 gaactacgca tgctcgagat gcccgcaaaa aacgttgccg tggtagcacg agtgttgggc
      301 gagaagattc agctcattgg tgaagaattg gtgcttgtac cggccgactc ggtaaaagca
      361 attggcggaa ttctccacaa agaaaatgga aaatgcggtg gcttcattga cgtcactgat
      421 gaatccatga gccactttga aaaaagtgtg gccggtctct caaaattcaa aacggatgaa
      481 tccgaatggg aagaggagca agaaccactg cctccactct acgaaatcga tcaaaccatc
      541 gttcaagtgg taaagtcagt gattcccaaa aacatagaaa attttgcgaa tgcttatagc
      601 aaaacattca ccacgcggta cgcggcttcc gaagagggtc ttgaggctcc gaagtggctc
      661 gccgaagtat ggacaaaaat ggcacacgac gcaaaccgat cggacattct cgtcgaacta
      721 atcaatcccc ataaacgcta cgaacaaagc agcgtgcgcg taacaattcc aggaactagc
      781 tccgaacgtg ctattgtggt gatcggcgca cacctcgaca gtatcaatca aggcgcatct
      841 gattttgcac cgggagtcga cgacgacgca tcgggaattg caacgatgac ggaaactttt
      901 cgagtgttgt tggcccataa ttttcggccc agcgcgacag ttcagttttt cggttatgcc
      961 gccgaggaag ttggactaat cggcagtcgg gctattgcgg agtcctatag aaattacgga
     1021 gccaaggtag cgggcgttat gcaactcgac atgactggtt ttccaggtcg caccaacgcc
     1081 gttactttca tacgtgatca cacaagcgat gagctcacca cttggaccca ggctgtgttt
     1141 aatatgtatg tgggtctggc gttcgtaaat gatcgctgcg ggtatgcctg cagcgatcac
     1201 gcttcgtggc accgctataa gtaccgaagc gtgttcccac atgaagctga atttaatgaa
     1261 tccaacaacc gcattcacac cgatcacgat ctttgggacg aacatatgaa tgcggagcac
     1321 gcgggtctat tcgcgaggct ggcagttgct ttcactctag agttagccaa atagaaaacc
     1381 cggcgggatc tgcccttttt caaggggccc acgccgggtt ttttcgttca tctgtcactg
     1441 ggccggacca ctcgagttcg gcctgattaa agcattaaag ctttgatttt ataggactat
     1501 ttagtttttt ggaaccttaa caagaccgga ctatggcctc aaccgggcgg tgaacgctcc
     1561 gtggaagctg ccaactgggc gcaaagatgc tccctggctt tttcgccgga tgacctccgc
     1621 atttcggtac ggaagagcgc ccacgagtct aaacaaattt ctcgcagtcg cattgcggat
     1681 ccggtcgat
//
LOCUS       DAOMXO010000562         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42306_length_1687_cov_4.596201, whole genome shotgun sequence.
ACCESSION   DAOMXO010000562 DAOMXO010000000
VERSION     DAOMXO010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42306_length_1687_cov_4.596201"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..841)
                     /locus_tag="PLH57_10340"
     CDS             complement(98..841)
                     /locus_tag="PLH57_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03050.1"
                     /translation="MMFFLGPAPGLSANPQDCSATLSEIATLDQINWVGNARPSEALF
                     QNTARTLPKSLNPLTRGTTLYALLSFQSFKAAAASSSEIKVVGTHLTTSIARRPDVVQ
                     KIEANGEYEFSLLAHSGETTPPQEVLQFRVLSGKTAHFTGTVFRGTSNQVLAGRRHDE
                     INLLLKRAAGSEHPIEEIELNHTHPMFQFVEVLAQPRPRFFITPLSNSDLALAVRYSI
                     DYPEVVFTIKAITPGGFYYKTSFKNGRTL"
     gene            complement(950..>1687)
                     /locus_tag="PLH57_10345"
     CDS             complement(950..>1687)
                     /locus_tag="PLH57_10345"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRK03051.1"
                     /translation="AKAAATPPPIFIRANFKFHVLGQEFSFLNQAISVFGVSALKWSY
                     FEPISRVGELVSISVDVLARERFKFTTTGSLMAEQTVDANMLSIRFHLQPQDKVKLEH
                     AIAVEGFCPTSHMRKYPRIPAVQKISDMPLRAIAQVDDNDQMVFDIANLSPNGILLYT
                     ENPKGGIYLPGNRVRGQIEPRGSYADSIHFEGIVRRTFQERNLDSQNLAHYLGVRFSH
                     IPPSQQDRFVDLLRSVLHDIKQLNRNS"
BASE COUNT          417 a          383 c          469 g          418 t
ORIGIN      
        1 caggaaccgg ttcgcccgcg ctcttgcgac tcaccggaaa acgtttaaaa ccaattttcg
       61 gttccgtcta taacttcgag gtcaagatcc atgcttttta gagcgtgcga ccatttttaa
      121 aagacgtttt gtagtaaaaa ccacccggcg tgatggcctt aatcgtgaaa acgacttcag
      181 ggtagtcgat ggagtaacga acggcgagcg ccaaatccga attcgaaaga ggcgtaataa
      241 aaaatcgggg gcgtggttgg gctaagactt caacgaactg aaacattggg tgagtgtgat
      301 tcagttcaat ttcctcgatt gggtgttcgc tccctgccgc tcgttttaac aaaagattga
      361 tctcgtcgtg ccggcgtccg gctagaactt ggttactggt tccgcgaaac acggttccgg
      421 tgaaatgcgc agttttgccg cttagtaccc gaaattgcag aacctcctga gggggagtgg
      481 tctcaccact gtgcgcaagc agcgaaaatt cgtattcgcc gttggcttca atcttttgta
      541 cgacgtccgg ccggcgcgca attgaggtgg taagatgggt gccgactact ttaatctcac
      601 tcgacgatgc ggccgccgct ttaaatgatt gaaaactgag aagtgcgtat aaagtagtac
      661 ctcttgttaa cgggttcaga ctcttgggta aagtacgagc ggtgttttgg aagagagcct
      721 cggaggggcg cgcgtttccc acccaattaa tttgatcgag cgttgcgatt tccgacaggg
      781 tggccgaaca atcttggggg ttggccgaga gccccggtgc gggccctaaa aaaaacatca
      841 aaaaaagtgt tggccccgcc caaaggggga gcttccaaaa aaacatctgg ccattatcaa
      901 tatatgacaa ataaagtcaa atattaaaaa gacctgtaat tcttgaggtt taggagttgc
      961 gattgagctg cttaatgtcg tggagcaccg aacgcagtag atcgacgaac cgatcctgct
     1021 ggcttggtgg gatgtgggaa aagcgcacgc ccaggtagtg ggccaggttt tgagagtcaa
     1081 ggttgcgttc ctggaaggtg cgtcgcacga tcccttcgaa gtggattgaa tccgcgtagc
     1141 tccctcgtgg ttcgatttgg cctcgtacgc gatttcccgg caggtagatg cctcccttag
     1201 ggttttcggt gtagagcagt atgccgtttg gcgagaggtt tgcgatgtca aaaaccattt
     1261 ggtcattgtc gtcgacctgc gcaatggcgc gcagaggcat gtctgaaatt ttctgtacgg
     1321 cagggattct gggatatttt cgcatgtgac tggtcgggca aaaaccttcg actgcgatcg
     1381 catgttctag ttttactttg tcctgcggct gtaaatgaaa acgaattgaa agcatattgg
     1441 cgtcgaccgt ttgttcggcc atgagcgagc ccgtggtggt aaatttgaag cgttcgcgag
     1501 cgagtacgtc gacggaaatt gagaccaatt cacccacgcg actaattggt tcgaaataag
     1561 accacttgag agcgctaacc ccaaacaccg aaattgcctg atttaaaaag ctaaactcct
     1621 gccccagaac gtgaaacttg aagttcgcgc gaataaagat cggtggcggt gtagccgcgg
     1681 ccttagc
//
LOCUS       DAOMXO010000563         1685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42383_length_1685_cov_4.394479, whole genome shotgun sequence.
ACCESSION   DAOMXO010000563 DAOMXO010000000
VERSION     DAOMXO010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42383_length_1685_cov_4.394479"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLH57_10350"
     CDS             complement(<1..633)
                     /locus_tag="PLH57_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRK03052.1"
                     /translation="MATPTVRRLKKGELLFNEGDPSKAMYFVQGGSIRLFKRKGTGSI
                     ELGSIHKGEVIGEMGFLDGGPRSASAEAVQDVELLEISNVNLAEQLKSMPPWLPVLLR
                     TVVNRLRSANNKIRQLESASTAYTYGSEGVSTNYQFLSVYDVMKICTALLVVSSRGDG
                     RCPMGRILRYANQIIGIHENKVAELLDVLERVGLVAIDKPAPDKIEVFVKD"
     gene            complement(637..>1685)
                     /locus_tag="PLH57_10355"
     CDS             complement(637..>1685)
                     /locus_tag="PLH57_10355"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRK03053.1"
                     /translation="GALFAGLVISGMVYFDQVILFNRNNIVFSGYLQAMQFLFQFIAI
                     TIFKYMREENEKKFIRSAFDKYVSPAVVDSMLKDPKKLKLGGEKIDLSILFSDIRGFT
                     ELSEKVDVKQLTSFLNEYLGAMTEILQANQGTLDKYIGDAVMGFWGAPVENTNHAMCA
                     VKTAIEMVAKLDELNKSFKEKYGFTIDIGIGINSGAVSVGNFGSNKVFEYTVIGDNVN
                     LASRLEGVTKYYGAKIVISDTTHAALKPGAFATREIDTVRVKGKHKPVKIYEVLPDAP
                     AFQGVRSGLEHFNSGLSNYYAQNWEQAIDRLEAVLKVKKDDRPTLELIERCKHYKNNP
                     PAQDWDGSWEMTSK"
BASE COUNT          413 a          395 c          379 g          498 t
ORIGIN      
        1 gtctttaaca aaaacttcaa tcttatcggg cgcaggtttg tcgattgcga ctaaacccac
       61 tcgttcgaga acatccagga gttcggcaac tttgttttcg tgaataccaa taatttgatt
      121 ggcatatcgt agaatgcgcc ccatcgggca gcggccgtcg cctcgcgacg acacaactaa
      181 aagtgctgtg cagattttca tgacgtcgta aacgctgaga aactgataat ttgtactaac
      241 accttccgac ccataggtat aagcagtgga tgcactttcg agttgacgaa ttttgttgtt
      301 ggcggaacgt aatcgattga ccacggtgcg aagaagcacg gggagccacg gcggcataga
      361 cttgagctgc tccgctaagt ttacgttact gatctcgagt agttcgacat cctgtaccgc
      421 ttcggccgag gcgctgcgcg ggccaccgtc tagaaacccc atttcgccga tcacttcgcc
      481 cttgtgaatg ctgccaagtt cgatcgatcc ggtgcctttg cgtttgaata gccgaataga
      541 accaccctga acgaaataca tggccttcga ggggtcaccc tcgttgaaca acaattcgcc
      601 tttttttagt cgtcgtactg tgggggttgc cataatttat tttgaagtca tctcccagct
      661 gccgtcccag tcctgcgctg gcggattatt tttatagtgt ttgcaacgtt ctattagctc
      721 tagcgtcggg cgatcgtctt ttttcacctt taatactgcc tctaggcgat cgatcgcttg
      781 ctcccagttt tgcgcgtagt aattggagag cccgctatta aaatgctcta gaccactgcg
      841 aacaccttga aaagccggtg cgtccggcaa tacttcgtaa attttcactg gtttatgctt
      901 cccttttacg cgaactgtgt cgatttcgcg ggtggcaaac gctccaggct ttagggcggc
      961 gtgggtcgta tcactgataa cgattttggc accgtagtat ttagtgacac cttccaggcg
     1021 tgaagcaaga ttcacgttgt cgccgataac cgtatactca aatactttgt tagagccgaa
     1081 gtttccaaca ctaactgctc ccgagttgat gccaatacca atgtcaatcg tgaaaccata
     1141 tttttctttg aaacttttgt tgagctcgtc aagtttagct accatttcga tcgcggtttt
     1201 tacggcgcac atcgcgtgat ttgtgttttc taccggtgct ccccaaaaac ccatgacggc
     1261 atcgccgata tatttgtcga gagtgccttg attggcttgg agaatttcgg tcatagcgcc
     1321 gagatattca tttaaaaagc tggttaattg tttgacgtct actttttcag aaagttcagt
     1381 aaatccccgg atgtcactaa ataaaataga tagatctatt ttttctccac cgagctttaa
     1441 tttctttgga tctttcagca tcgagtctac gacagcgggt gagacgtatt tatcaaacgc
     1501 tgatctaata aattttttct cgttttcttc gcgcatgtat ttaaaaatcg taatcgcgat
     1561 aaactgaaat agaaactgca ttgcttgtag gtaaccggaa aaaactatgt tgttgcgatt
     1621 gaagagaatg acttgatcaa aatataccat tccagagatt actaggccgg cgaatagagc
     1681 tccta
//
LOCUS       DAOMXO010000564         1683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42461_length_1683_cov_3.962531, whole genome shotgun sequence.
ACCESSION   DAOMXO010000564 DAOMXO010000000
VERSION     DAOMXO010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42461_length_1683_cov_3.962531"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLH57_10360"
     CDS             complement(<1..305)
                     /locus_tag="PLH57_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03054.1"
                     /translation="MFLLGFAALVRSFILPGLILQRVFKFRSNWMVQLFFSVPFSLLF
                     THYLVFFLTACGWYLRPVVLSLFAVQCIALFALIKLENRRKSSESPFKFLDPSVFEN"
     gene            complement(320..1645)
                     /locus_tag="PLH57_10365"
     CDS             complement(320..1645)
                     /locus_tag="PLH57_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK03055.1"
                     /translation="MAQISKDAELFQKFLPGRKILIADSNAGSRSSLFNVLKEMGAKS
                     NQLFLANTYKAAEEQIAELKPHVVIAEYEFGPRCGLELLQSQREQRPKETKDCIFIIV
                     TGNTSQTAAARALEEDIDAYIIKPYTLEFVRKTILNAAIQKIRPPEYLKTIEQGKARM
                     EEAKLDEAEELFNKATKLDPAPSLAYYYLGQVKFLREIVAQAQSNYMKGLKFNGIHYK
                     CLVGMYELLMKERRHKDAYDVVKKIAKYFPANPKRLGEVLRLAIMTGEYEDIERYYLM
                     FMTLDQRNETLIRYTTAALVVCGKYYLDSNQGAGKALDIFNKAAVTSMGSPRVLSEII
                     PVLIENNLPEEAEKFLKRFPPETHKGKEFQVMNFLILNGKSAPSLVVEQGRRLLAQGI
                     EDPRLYEVMIKRSVEANLGAAAEDLVRSAVAKYPAKKELFESLLKRSSP"
BASE COUNT          455 a          397 c          335 g          496 t
ORIGIN      
        1 aaattttcga aaacactcgg gtccaaaaat ttgaacggcg actcagagct cttacgtcta
       61 ttttccaact ttatcagcgc aaacaacgca atgcactgaa cggcgaacag cgacagcacc
      121 accggccgca aataccagcc acaggctgtt agaaaaaaca ctagatagtg cgtgaacaaa
      181 agactgaagg gcaccgaaaa aaacagttgc accatccaat tcgatcgaaa tttaaaaacc
      241 ctttgtaaaa tgaggcccgg tagaatgaac gatcgaacaa gagctgcgaa cccgagaaga
      301 aacatttttg aacgatatat tacggactac tgcgtttcaa taacgattca aataactctt
      361 ttttggccgg atattttgcc actgcacttc tcaccagatc ttcggctgca gcgccaagat
      421 tagcttctac tgaacgttta atcattactt cgtagagtcg cggatcttca atgccctggg
      481 ccaataaacg ccggccctgc tctaccacca gtgaaggggc cgacttgccg tttaaaatta
      541 gaaaattcat tacttgaaac tctttgccct tatgcgtttc tggtggaaac cgttttaaaa
      601 atttttcggc ttcctcaggc aaattatttt caataagcac gggaatgatt tctgacaata
      661 ctcttgggct acccatcgag gttactgccg ctttattaaa aatatcgagg gcctttcctg
      721 caccttggtt tgaatctaaa tagtactttc cgcacactac taaagcggcg gtcgtgtatc
      781 gaatcaacgt ttcgttacgt tgatccagcg tcatgaacat caaataataa cgttcgatgt
      841 cctcgtattc tccggtcatg attgctaagc gcaacacttc tccaaggcgc tttgggttgg
      901 caggaaaata tttagcgatt ttctttacga cgtcgtaggc gtctttgtgt cgccgctctt
      961 tcattaatag ttcgtacatt ccaactaaac atttgtagtg aatgccgtta aattttaggc
     1021 ccttcatgta attagactgg gcctgagcaa caatttctcg taaaaacttc acctgaccaa
     1081 ggtagtaata agcaagcgac ggcgccggat caagtttcgt agctttgttg aaaagctcct
     1141 cagcctcatc gagttttgcc tcctccatgc gggccttgcc ttgctcgatt gtttttaaat
     1201 actctggcgg tcgaattttt tgaatcgctg cattcagaat tgtctttcga acaaattcaa
     1261 gggtgtaggg cttaataata taggcatcga tgtcctcttc taatgctcga gctgcagcag
     1321 tctgcgacgt atttcccgtc acaattataa atatgcaatc ctttgtttct ttaggacgtt
     1381 gctctctttg actttgaagc agctccaaac cgcagcgcgg accaaattcg tactcggcaa
     1441 ttacaacgtg cggtttgagt tctgctattt gttcttcggc agctttatag gtgttcgcta
     1501 gaaacaactg attcgatttg gcgcccattt cttttaaaac gttaaataga cttgaacgag
     1561 accccgcatt gctgtccgca attaaaattt ttcttccggg gagaaacttc tgaaatagtt
     1621 ccgcatcttt tgatatctgc gccactattt attcctcatc tcgaacaatc gagatatctc
     1681 ttg
//
LOCUS       DAOMXO010000565         1681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42522_length_1681_cov_4.794588, whole genome shotgun sequence.
ACCESSION   DAOMXO010000565 DAOMXO010000000
VERSION     DAOMXO010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42522_length_1681_cov_4.794588"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..798
                     /locus_tag="PLH57_10370"
     CDS             136..798
                     /locus_tag="PLH57_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03056.1"
                     /translation="MELRNKKTMDFKKLLTKQLLISLIWTSTISSSRASELAVQLPHK
                     APRDCEKITHRFLGELRSIAPDVTITVDVIEHQKVGSSAQKITLQCTGLNTLTVNFSA
                     LESKQDFIFRYLDSHDTFQAEDWIPFQQMVFSKLEPSNSQPPVDSDLNQLRDVAPTYS
                     AVEKTNPSLLQTTTPTEVAANESGLLKKWWFWSLVAVAGGGTYWVVKNQSRATSATIQ
                     VR"
     gene            complement(786..1538)
                     /locus_tag="PLH57_10375"
     CDS             complement(786..1538)
                     /locus_tag="PLH57_10375"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF004079.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRK03057.1"
                     /translation="MKTKIGILSALDGEIESIQKYLKSHSRNVEETRLAGRIYQTTSI
                     QSPRGPLEVVSVVGGWGKVASAVAATTLLAKFNVEQLLFWGVAGSCSRILKPGDVVIG
                     DRCMQYDVDARPFVGLGELPFVGRPWLELDVGLSEELSQVAGVAAETLRKDPRPRASI
                     ALGPIVSGDQFLSRLEQGESLLQRDPSILAADMESAAVAQVCAEFGVPFGVVRVISDR
                     IGLKPSESFRSFLSQWASPLCAEMFKQWIYRT"
BASE COUNT          463 a          459 c          350 g          409 t
ORIGIN      
        1 tacaaggcca ggactatcga atagacctcg gaattcctcg cagggacaaa gcattattga
       61 gttcgtcgtt tttctcacag cattcaccct aattttcgga gcgtttgcca accaatttaa
      121 aaggagctca ctaaagtgga actacgaaat aaaaaaacaa tggatttcaa aaaattacta
      181 accaaacaac tactaattag cttgatttgg acatcaacaa tctctagttc tcgtgcctcg
      241 gagcttgcag ttcaattgcc tcacaaggca ccccgagact gcgaaaaaat cactcaccgg
      301 ttcttaggtg aacttcgctc cattgctccc gacgtgacca ttacggtcga cgtaatcgaa
      361 catcaaaaag tggggtcgtc tgcccagaaa attactctcc aatgtacagg cctcaatacc
      421 cttacggtca attttagtgc attagaatcc aaacaagact tcatatttcg gtatttggat
      481 tctcacgaca cttttcaggc cgaagactgg attccatttc aacaaatggt tttttcaaaa
      541 cttgaaccta gtaattccca accccccgtt gattccgatc tcaatcaact acgcgacgta
      601 gcgccgacct attccgcagt ggaaaaaact aaccccagct tgctacagac aacaacaccg
      661 actgaggttg cagccaatga atctggtctt ttaaaaaaat ggtggttttg gagcttggtc
      721 gcagttgcag gtggcggcac ttattgggtc gtcaaaaatc aatcacgagc caccagtgcg
      781 actattcaag tgcggtaaat ccactgttta aacatttcgg cgcaaagtgg gctggcccat
      841 tgacttagaa acgatcgaaa ggactcgcta ggttttaggc caatacggtc tgaaataacc
      901 cgtaccactc cgaacggcac gccaaattcc gcacaaactt gggccactgc tgcgctttcc
      961 atatctgccg caagaatact ggggtcgcgc tgcaacaacg attcgccttg ttccaagcgt
     1021 gacaaaaact gatcaccgga tacgatcggg cccaaggcaa tgctcgctcg cggtcgtgga
     1081 tctttgcgca gtgtttccgc cgcgacgccc gcgacttgcg aaagttcttc gctgagtcca
     1141 acgtctaact ccagccatgg ccgacccaca aatggcaatt cgcctagacc gacaaatggc
     1201 cgggcgtcta cgtcgtattg catgcagcgg tcacctatta cgacgtctcc tggttttaaa
     1261 attcgggaac atgagccggc cactccccaa aaaagtaact gctccacatt aaacttggcc
     1321 aaaagagtag tggcggccac cgccgaagct accttccccc accctccaac cacagagacc
     1381 acttccagcg gacctcgagg ggattgtatt gaggtagttt gataaatgcg acccgccaaa
     1441 cgtgtctctt cgacgtttcg cgagtgggac ttcaaatact tctgaatact ctcaatttcg
     1501 ccgtccaaag cactcaaaat gccgatttta gttttcatct cattttcttt tacggtagaa
     1561 gcagcccatg attccagaac ttattaagaa aaacactcca caacatcgaa tgctcgccgt
     1621 cggatcgctt gctttcgact ctattcaaac tccggcagga aaggttgacc gcatcctcgg
     1681 t
//
LOCUS       DAOMXO010000566         1680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42560_length_1680_cov_4.499077, whole genome shotgun sequence.
ACCESSION   DAOMXO010000566 DAOMXO010000000
VERSION     DAOMXO010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42560_length_1680_cov_4.499077"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLH57_10380"
     CDS             <1..759
                     /locus_tag="PLH57_10380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK03058.1"
                     /translation="GIPTFLMPVYHGTTAGRMIACREYFSPAGYRSLLKEFKIALRAN
                     AAVCVSPLAEQEARRYYFLRCPTVVIENAADVEIFRPLKAISEEPRILFIGRSGDRVK
                     NTDRILEAVSQVAKKGTRIILSAVPGISNTNSNNIESLGPKNSFDLYKLFQSSRALIL
                     GSLYEGDPIVLHEAKSAGLPILASDLPQMRYALLNYKNSYFFNPRNTDAIANQIESLL
                     ADPNLKPCSLASSWSNSAKKLLNFYSQQIAAQRS"
     gene            complement(719..>1680)
                     /locus_tag="PLH57_10385"
     CDS             complement(719..>1680)
                     /locus_tag="PLH57_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03059.1"
                     /translation="FHAPSLHDRLRPWYPLKSALMLRAAFPKSKFISLVHEFSEAPAH
                     WKIRQIAITKLATAVVVNSEADETGMSRWHSRLLRIPLGPTLSLEGLPKSELDLNRNL
                     VESAVRNFRKQVFSDLNLAEGAKLIVHPGLVTPGKGVNSLEQMLNSFENATKNFHLVV
                     VGGFGPKERDRVFAMETTRRLSAAAKGRFSFIESPSDVRFQQLLTAADLVVLPYDEGV
                     SERRSSFLSSMWSAANVWITTGNFSAPLRLESSAAHSVSKSEFQQSPTVVSESIKKAL
                     AESYENAMERRLKNLNWAYQRSWGKRSEKFANFVEQLFVGNKN"
BASE COUNT          460 a          419 c          349 g          452 t
ORIGIN      
        1 ggaataccta cttttctaat gccggtttac cacggcacaa ctgcagggcg catgatagcc
       61 tgccgcgaat attttagtcc tgctggatat cgttccttgc ttaaggagtt caaaatcgca
      121 ctccgagcta acgctgccgt gtgtgttagc ccattggccg aacaggaagc acgacgatat
      181 tattttttaa ggtgtcctac cgtcgtaatt gaaaatgcag ccgatgtaga aatatttaga
      241 cccctcaaag ccatttccga ggagcccaga attttattta tcgggcgcag cggggatcgt
      301 gtaaaaaaca ctgaccggat tctagaagct gtctctcaag ttgctaaaaa gggaaccagg
      361 atcatacttt cggccgtgcc aggcatttca aataccaaca gtaacaatat cgaatccctc
      421 ggccccaaaa atagcttcga tctttacaaa ctatttcaat ctagccgcgc tctcatttta
      481 ggctcgcttt acgaaggtga tcccattgtc ctccatgagg caaaaagtgc gggtttaccg
      541 attctagcgt ctgatttgcc tcaaatgagg tacgcgctac taaattacaa aaattcctat
      601 ttttttaatc cgcgcaacac tgacgccatc gccaaccaaa tagaatccct acttgcagat
      661 ccaaatttaa aaccctgctc attggcaagt agttggagca attctgcaaa aaaattactt
      721 aatttttatt cccaacaaat agctgctcaa cgaagttagc aaatttttcg ctccgtttac
      781 cccaagacct ttggtaggcc caatttaaat tttttaatct gcgctccatg gcattttcat
      841 aggattctgc tagcgctttt tttatacttt cgcttactac agttgggctc tgctgaaatt
      901 cagactttga aacggaatgt gccgcgctag attctagtcg aagtggtgcc gaaaaattac
      961 cggtcgttat ccaaacattg gctgcgctcc acattgaaga tagaaacgac gaacgcctct
     1021 ccgacactcc ttcgtcatac ggaagcacta ccaaatcagc tgccgttagc agttgttgaa
     1081 aacgtacatc gctcggtgat tctataaaac taaaacgtcc cttggcggct gcacttagcc
     1141 gccttgtggt ttccattgca aacacacgat cacgttcttt cggaccgaac ccaccgacga
     1201 ccacaaggtg aaaatttttg gtggcatttt cgaaactgtt caacatttgc tcaagagagt
     1261 tgaccccctt gccaggagtg actaaccccg gatgaacaat taatttagcg ccttccgcga
     1321 gatttaaatc cgagaacact tgttttctga aatttcgcac cgcactctct actaaattcc
     1381 gatttagatc taattccgat ttaggcaaac cctcgagcga tagcgtcggg cccaagggga
     1441 ttcttaacaa tctcgaatgc caccgagaca tgcccgtctc gtcagcctct gaattcacca
     1501 caacagcggt tgccaatttg gtaattgcga tttgtctaat tttccaatgt gcgggggctt
     1561 ccgaaaattc gtgaacgagg ctgataaatt tcgacttagg aaacgccgcc cgcaacataa
     1621 gggcagactt aagtgggtac cacgggcgca gtcggtcgtg aagagaagga gcgtgaaata
//
LOCUS       DAOMXO010000567         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42588_length_1679_cov_6.227217, whole genome shotgun sequence.
ACCESSION   DAOMXO010000567 DAOMXO010000000
VERSION     DAOMXO010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42588_length_1679_cov_6.227217"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLH57_10390"
     CDS             <1..825
                     /locus_tag="PLH57_10390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRK03060.1"
                     /translation="GHFGREEPEFTWEATDKAVPGSDGNEAAHSWSVGTRVSMPEEKT
                     AHLRRVLRLEHGQEVLVTDGRGQLYGAVFEVEGKKGFAQIRGLLRSEKKEHDVTLILG
                     LPKNNTMDWVLEKAVECGASRIVPVETARSVVKVGAERGDKYLKRWGAIAEAAIEQSE
                     RLWGVEIAEPVSWNDFLSSYQAPGHPVRIACFSESREEYEDQRIPLQEFWKAMADCQN
                     RPIEAWIGPEGGFTESERKIIVERGFVPVSLGQQVLRVETAAVAVLWSIVLRRALG"
     gene            834..1454
                     /locus_tag="PLH57_10395"
     CDS             834..1454
                     /locus_tag="PLH57_10395"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRK03061.1"
                     /translation="MEQKTKDQEPEIAFRPLTEGLGINHFSDGLPYANAGQTPARRPQ
                     GHFDFPPPRAPRATAPAKQEVVTDLRVESEVDLKLTPVAPLATGTLAPISTRLIAYLC
                     DLGFVALFYIVVMSAGLALAGVSISSFAEGLWAFRQSHPALDLIVSMFGLIYFGYFLL
                     QEVSWGTTLGKSLFRLRLKGGSVFTRALRVTLFLAPITGVSVVREA"
     gene            1459..>1679
                     /locus_tag="PLH57_10400"
     CDS             1459..>1679
                     /locus_tag="PLH57_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRK03062.1"
                     /translation="MTIRLNLKNKIKSPSQLAKITQALRKKGKCVVFTNGCFDILHPG
                     HVTYLQEARNKGDVLVIAVDTDASVRKLKG"
BASE COUNT          415 a          374 c          478 g          412 t
ORIGIN      
        1 ggccacttcg gccgcgagga gccggaattc acctgggaag ccaccgacaa ggcggtaccg
       61 gggtccgacg gaaatgaagc agcgcattcg tggagcgtgg gtacccgtgt atcgatgcca
      121 gaggaaaaaa cggcacatct tcgacgagta ttgcgccttg aacatgggca agaagttctg
      181 gtgactgatg gacgcgggca gttatacggc gccgtgtttg aagtagaggg taaaaaaggc
      241 tttgctcaga tcaggggcct attgagaagt gaaaagaaag agcacgacgt gaccctgatt
      301 ctagggttac caaaaaacaa cacaatggac tgggtactag aaaaagcagt cgaatgcggt
      361 gcgagccgca ttgttccagt tgaaacggcg cgcagcgtag taaaggttgg cgccgagcgc
      421 ggtgataaat atttgaaacg ttggggtgcg attgcggaag ccgcaattga acagtcggag
      481 cggttgtggg gagtagaaat agccgaaccg gtttcttgga atgatttttt gagttcttat
      541 caagctcctg gccatccagt acgaattgcg tgtttttcgg aaagtcgcga ggagtatgag
      601 gaccagagga ttccgttgca ggagttttgg aaggcgatgg ccgattgcca aaatagaccc
      661 attgaagcct ggataggacc cgaaggcgga ttcactgaat cggaacgtaa aatcatcgtt
      721 gagcgggggt tcgtacctgt cagtttaggc caacaggttt tgagagttga aaccgcggcg
      781 gtggcggtct tatggtcgat tgttcttcgc agagctttgg gttagaaata gacatggaac
      841 aaaaaaccaa agatcaggaa ccggaaattg cgtttcgccc actaaccgag ggactcggaa
      901 tcaaccactt ttccgacggc ttaccttacg caaacgccgg acagactccc gccaggcggc
      961 ctcaggggca tttcgacttc cctcctcctc gagcgccaag ggctactgcc cccgctaaac
     1021 aagaagttgt gacggacttg cgggtagagt ccgaggtaga cctaaaattg actccggtcg
     1081 ctccacttgc aacgggtacg ctcgccccaa tttccacgcg ccttatcgcc tatttgtgtg
     1141 atctgggttt tgtggcttta ttctatatag tcgtaatgag tgcggggctc gccttagctg
     1201 gcgtgtctat ttcatccttt gcggaaggcc tttgggcttt tcggcaatct cacccggcgc
     1261 tcgatttaat agtttctatg tttggattaa tttattttgg gtatttcctt cttcaggaag
     1321 tttcttgggg aactactctc ggtaagtcgc tttttcgtct acggctcaag ggcgggtctg
     1381 tgtttactcg tgccctcagg gtaactttat ttttagcccc gattacaggt gtttcggtgg
     1441 ttcgagaagc gtgagatcat gacgatccga ctaaatctta aaaataaaat taaatcgccc
     1501 tcgcaattag cgaaaataac ccaggcgcta aggaaaaaag gcaagtgtgt ggtttttacc
     1561 aacgggtgtt tcgatatttt acacccggga cacgtaacct atttgcagga agcgcgaaac
     1621 aaaggcgatg tattggtaat cgcggtcgat accgatgcgt ccgtacgtaa actcaaggg
//
LOCUS       DAOMXO010000568         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42812_length_1673_cov_5.567985, whole genome shotgun sequence.
ACCESSION   DAOMXO010000568 DAOMXO010000000
VERSION     DAOMXO010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42812_length_1673_cov_5.567985"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PLH57_10405"
     CDS             complement(<1..851)
                     /locus_tag="PLH57_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRK03063.1"
                     /translation="MRVYENWIAGAVRPANQKVFVASPWSGRTVAEVEFAEPADLELA
                     LTTAFDARALLRKTTRSQRAEFLNAIADALEERRELFVKILIEEAGKPIDLADVEVTR
                     AVSTFRIAAAEVRGFVGRKIALDSWGNSARLHDGFTVWEPRGVVLGITPFNFPLNLVA
                     HKVAPAIAAGCPIIVKPAPKAPGGAHVLCAEIGALASRNEFKAFVHAVQCVSQTNELT
                     SVANADPRVAVLSFTGNDVVGWALKTQAPARRHATLELGGNAAVVIDLSADLDFAAER
                     VAWGAFS"
     gene            complement(848..1372)
                     /locus_tag="PLH57_10410"
     CDS             complement(848..1372)
                     /locus_tag="PLH57_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HRK03064.1"
                     /translation="MILATPPVRLNSAVLAVLVYLFDRDGRVLMIHRNADPNDYHFGK
                     YNGLGGKLDALESPLQAAVREVYEESGLSVRPEDLKPCGYVHFPNFKPAKAQDWTVFI
                     FTGQYAGSEPPKQKCREGTLQWVPVSSVLELNLWPGDAHFLPAVLGQQTVSGAIWYDA
                     DGNVTKVEMREEQK"
     gene            1411..>1673
                     /locus_tag="PLH57_10415"
     CDS             1411..>1673
                     /locus_tag="PLH57_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK03065.1"
                     /translation="MSTKRRALIFGVANERSLAWAIAQKLAADGCETYFTYQGERLER
                     RVKPLASEVGASLVEQCDVADPAQLDRFFQLLAQKTDHIDILI"
BASE COUNT          381 a          478 c          424 g          390 t
ORIGIN      
        1 aaagaaaaag ccccccaggc cacgcgttcc gccgcaaaat caagatcagc cgaaagatca
       61 atcacaactg cagcgttgcc accgagttcg agtgttgcgt ggcgccgagc cggcgcctgg
      121 gtttttaaag cccaacctac tacgtcgttg ccagtgaaac tcaggaccgc cactcgtggg
      181 tcggcgttcg cgacagacgt tagttcgttt gtctgcgata cgcattgcac ggcgtgaacg
      241 aatgccttga actcgttgcg agaagcaagc gccccgattt cggcgcacaa cacatgggct
      301 ccaccgggcg ctttgggggc gggcttaact atgatcgggc accccgccgc tattgcgggt
      361 gcgactttat gggccactaa attgagtgga aaattaaagg gcgtaatccc gagcaccacg
      421 cctcgtggct cccacactgt gaaaccgtcg tgtaggcgtg cgctattgcc ccaactgtcg
      481 agcgcgattt tgcggccaac aaaccctcgc acttcggccg ccgcgattcg aaaggtactg
      541 acggctcggg tcacttccac atcggcaagg tctatgggtt tgccggcttc ttcgattagt
      601 atttttacaa acaattcacg ccgctcctca agagcgtcgg cgattgcatt cagaaactcc
      661 gcccgttggg accgagtagt ttttcgcaac aacgcgcggg catcgaaggc cgtggtcaac
      721 gcaagttcta gatctgcggg ctccgcaaac tcgacctcag ccaccgttcg cccggaccac
      781 ggcgaagcta caaatacttt ttgattcgcc ggccgcacgg ctcctgctat ccagttttcg
      841 taaactctca cttttgttcc tctcgcattt cgacttttgt tacattgccg tcggcgtcgt
      901 accaaatcgc accactgacc gtttgctggc caagtacggc aggtaaaaag tgggcatcgc
      961 ccggccaaag attaagttct aaaaccgagg agaccggcac ccattgaaga gtgccctcgc
     1021 ggcacttttg cttcggaggc tcactcccgg cgtattgtcc ggtaaatata aacacggtcc
     1081 aatcttgggc tttggcgggt ttaaaatttg gaaagtgcac atacccgcaa ggtttgaggt
     1141 cttcgggcct tacgctcaac cccgactctt cgtacacttc gcgtaccgcc gcttgcaaag
     1201 ggctttcaag tgcatcgagt ttaccgccca atccattata ttttccgaaa tgatagtcgt
     1261 tcgggtccgc gttacggtga atcattagaa ctcgaccgtc tcggtcgaaa aggtatacca
     1321 aaacagccaa aacggcggaa tttagtcgta ctggaggtgt cgctaatatc atacttgatt
     1381 gtcccttaat ctcgcggcaa tctgcggtct atgtcaacta aacgtcgtgc ccttatattc
     1441 ggtgtcgcca acgaacgcag tttagcttgg gccattgccc agaaacttgc cgcagatggc
     1501 tgcgaaacat atttcaccta ccaaggcgaa cgcctcgaac ggcgagttaa acctttggcc
     1561 agcgaagttg gtgcaagctt agtagagcaa tgcgacgtgg ctgaccccgc gcagctagat
     1621 cgattttttc agttgttagc tcagaaaacc gaccacatcg acattttgat cca
//
LOCUS       DAOMXO010000569         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42816_length_1673_cov_5.116193, whole genome shotgun sequence.
ACCESSION   DAOMXO010000569 DAOMXO010000000
VERSION     DAOMXO010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42816_length_1673_cov_5.116193"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLH57_10420"
     CDS             <1..637
                     /locus_tag="PLH57_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03066.1"
                     /translation="KALAPAGARLTPMLAITAQGFLFSLAVGLLGTRWVGLCFGATLL
                     AFWAVIQPIALGLIVFGASAVRAAEWLRQMVSSDLAIWGWWVFGGLIGLKVLLANLLV
                     VVAVKASSSSWGRFEEKLTSVQLERRSRNNVWPAIWWLVSGALLVTFSIVVEARAVAT
                     VWLLLRIGAGVLFVFWLRALLKEARVQRWILNRYPDLAHVLREVTGISRPN"
     gene            complement(543..1448)
                     /gene="xdhC"
                     /locus_tag="PLH57_10425"
     CDS             complement(543..1448)
                     /gene="xdhC"
                     /locus_tag="PLH57_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HRK03067.1"
                     /translation="MITRSFFRTAAELEQQNKAFVVVTLTATRGHAPQDAGAKALVTK
                     EGLAWGTVGGGKVEARAIEYAIELLATKRPATQSVNWNLQRDIGMSCGGEVEFLFETV
                     LPVEWKIFVFGAGHVGQALCRILSTLNCRVICVDTRTEWLERLPQDSENLISLRLADY
                     SAPSLASVASAQDLKCSFVVTTRGHSTDVPVLHWLSTIAPSPNVSFGYLGVIGSRVKA
                     LKIKNELRALGCSPAFIEQLKCPMGLDIGNSNDPAEIALSIAAELIQRRNLTFNLVEK
                     SPSPRAAHVPNLDTDSISTVELGPL"
     gene            complement(1448..>1673)
                     /locus_tag="PLH57_10430"
     CDS             complement(1448..>1673)
                     /locus_tag="PLH57_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03068.1"
                     /translation="NIQDTPRVFNVVLFENHANTANIRGTKAAGEPPLLLATSAWTAI
                     RNALAYLRPKPNLHTVPATSERILRALENF"
BASE COUNT          356 a          384 c          511 g          422 t
ORIGIN      
        1 taaagcgctt gcgccagcgg gcgcgcgact gactccgatg ttggcgatta cggcacaggg
       61 ttttttattt tcactagcgg tggggcttct cggaacgcgg tgggtgggtt tgtgttttgg
      121 ggcaactttg ctcgcgttct gggcagtgat tcaaccgatt gcccttggac taatcgtgtt
      181 cggagcgagc gcggtacgag ccgctgagtg gttgcgacaa atggtctcaa gcgatttggc
      241 gatctggggc tggtgggtgt ttggtggact gatcggactc aaagtcctgt tggccaattt
      301 actggttgtg gtggcggtga aggcttcgtc aagctcttgg gggcgatttg aggaaaagct
      361 aaccagcgtt caattagagc gccgatcgcg caacaacgtg tggcccgcga tctggtggct
      421 ggtgtcggga gcacttctcg tgacgttttc gatcgtcgtc gaagcccgag ccgttgcaac
      481 ggtgtggttg ttgttgcgca ttggggcagg tgttttgttc gttttttggc tgcgagcgct
      541 actcaaagag gcccgagttc aacggtggat attgaatcgg tatccagatt tggcacatgt
      601 gctgcgcgag gtgacgggga tttctcgacc aaattaaagg ttaggttccg tctttgaatc
      661 agttcggcgg caatgctaag cgcaatttct gcggggtcgt tggagtttcc gatatctagt
      721 cccatcggac attttagttg ttcaataaat gctgggctgc acccaagtgc gcgaagttcg
      781 tttttaattt taagggcttt cacgcgactg ccgatcacgc ccagataacc aaaggaaaca
      841 ttcggcgagg gagcaatggt agagagccaa tgtaatactg gaacgtcagt gctgtggccc
      901 ctcgtggtga cgacaaaact acatttcaga tcctgggccg aggccaccga ggcaagtgaa
      961 ggagccgaat aatcggcaag gcgaagagag attagattct cgctatcttg cggcaaacgt
     1021 tcgagccatt cggtgcgcgt gtcgacacaa attacgcggc agttgagcgt tgagagaatg
     1081 cggcataacg cttgcccgac atggcccgcg ccgaagacaa aaattttcca ttcaactggc
     1141 agaacggttt caaaaagaaa ctcgacttca ccgccgcaac tcatgccaat gtcacgctga
     1201 aggttccagt tcacggactg ggtggccggg cgcttggtgg caagaagttc aattgcgtat
     1261 tcaattgctc gcgcctcaac tttgccacca ccaacagtgc cccaggctag tccttccttc
     1321 gtgacaagtg ccttggcgcc tgcgtcttgc ggagcatggc cgcgcgtagc cgttagagtc
     1381 acaactacga aggccttgtt ttgttgttcg agttcggcgg cggttcgaaa aaaagaacgg
     1441 gtgatcatta aaaattctcc agagctctta atatacgttc gctggtggcc ggtacggtgt
     1501 gtagatttgg tttgggcctt aggtaggcaa gagcgttgcg aatcgcggtc caggccgaag
     1561 tggcaagtag tagcggcggt tcacctgcgg ccttggtgcc gcgaatattc gcggtgttgg
     1621 catgattttc aaaaagaacg acgttaaaaa ctcgcggagt atcttgaatg ttc
//
LOCUS       DAOMXO010000570         1671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42901_length_1671_cov_4.967203, whole genome shotgun sequence.
ACCESSION   DAOMXO010000570 DAOMXO010000000
VERSION     DAOMXO010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42901_length_1671_cov_4.967203"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..884
                     /locus_tag="PLH57_10435"
     CDS             243..884
                     /locus_tag="PLH57_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03069.1"
                     /translation="MASFRRKKEALRAVTYAFVAGVHYISTEVVRNLALRSEMGGGEN
                     QLSSNDVLNNYQNIFSSPQALDLFAEHLNEEGLYPFGSEWSIEVISELVGKLNPPSLL
                     EIQGEAAVIRFKKGYKALLDSCADTMESVRRNDELLDHWLTLLLNTSFFGAMSVAARC
                     SSNPKLYYSVLNEIETEIEPWSHYDQLVNLLDRQLRRETKRKTGLVTEHEFEQ"
     gene            complement(890..1501)
                     /locus_tag="PLH57_10440"
     CDS             complement(890..1501)
                     /locus_tag="PLH57_10440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRK03070.1"
                     /translation="MQLKKSKLFAVGLFLVYFVLRILFDDRWREISPYYSYLFEAGII
                     GAAIFVYQKRTAFKWPNLQNLVTFGCVALVAGFGAHDTAGVFGILIPFNLEGFETLFL
                     LLILAPVLEELLFRFALWEPLELFTKSGVVIVALTTLFFVSGHLMSYSYVPDEFKSFI
                     VYQAMYVILLGIGAGFARLKYGMPTAILVHFFFNLGFFVSAKM"
BASE COUNT          479 a          389 c          389 g          414 t
ORIGIN      
        1 ataaaggccc gaaaaaacgc gacttcccag ataggcacgt cacgggaagc cttaacgaac
       61 gctcccatga tcgaaaataa aacggcggcc ccgatcatcc atatggcggg gccaaggtaa
      121 gtggctaact tttttgatga tattcgtaac attcgtaaag tgtgggccca atataccgga
      181 ccgagttact ttaccatttt ctggacgcgc tggctatcgc tgtgttagtc atccacgcac
      241 ttatggcaag ttttagacga aaaaaagaag cgctacgcgc agtgacttac gcttttgtag
      301 ctggcgtgca ttatattagt accgaagttg tgcgcaactt agccttacgc tcggaaatgg
      361 gcggcggtga aaatcagctc agctcaaacg acgtgctgaa caattaccaa aacattttct
      421 cgtctccaca ggcgctcgat ttgtttgccg aacacttaaa cgaagagggg ctttatccct
      481 ttggttctga atggtcgatt gaggtcattt cggagcttgt cgggaaatta aacccacctt
      541 cgctgcttga gattcagggc gaagcggccg tcattcgctt caaaaaaggt tataaagcat
      601 tgctcgacag ttgcgcagat accatggaga gtgtgagacg caacgacgaa ctcctagacc
      661 actggttaac tcttttacta aacacttctt ttttcggcgc catgagtgtg gctgcgcgct
      721 gttcttcgaa tcctaaactt tattattctg tgctgaatga aatcgaaacg gaaatcgaac
      781 cgtggtcaca ttacgaccaa ttggtgaacc tgctcgatcg ccagttgcgc agagaaacta
      841 aacgcaaaac cggactggtg accgaacacg agtttgaaca gtaaaattgt tacatttttg
      901 cgctcacgaa aaatcctaga ttaaaaaaga agtgcactag aattgctgtt ggcattccgt
      961 attttagacg cgcgaagccc gcgccgattc cgagaagaat cacgtacatt gcttgataaa
     1021 caataaagga cttgaattcg tctggaacgt aggaatagga catcaggtga ccactcacaa
     1081 aaaacaaggt ggtcagcgct acgattacaa caccactctt cgtaaacaat tcgagcggct
     1141 cccaaagcgc aaatcgaaac aaaagttctt caagcactgg cgctagtatt aataacagaa
     1201 acagcgtttc aaagccttca aggttaaatg ggattaatat tccaaatacc cctgccgtat
     1261 cgtgggcccc aaatcctgcg acaagtgcga cgcaaccaaa cgtgactaga ttctgcaggt
     1321 tcggccattt gaacgcggtt cttttttggt acacgaaaat agccgcacca ataattccgg
     1381 cctcaaatag ataagagtag tacggcgata tttcgcgcca tcgatcgtca aataatattc
     1441 taaggacaaa gtagacaagg aataacccta ctgcgaagag tttggatttt ttaagctgca
     1501 tcctaagatt gtaaatcaag ttcgagggct gacataaaaa tactttgagg ttttttgagg
     1561 cttgtcagtt gggatatggc ggagagggtg ggattcgcct atctgtcttc gcgctattcg
     1621 cgcttaagca gacaggcccg cctcactcgc tgcccactgg cgtgggcacc c
//
LOCUS       DAOMXO010000571         1669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_42980_length_1669_cov_4.481413, whole genome shotgun sequence.
ACCESSION   DAOMXO010000571 DAOMXO010000000
VERSION     DAOMXO010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42980_length_1669_cov_4.481413"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="PLH57_10445"
     CDS             complement(<1..1467)
                     /locus_tag="PLH57_10445"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRK03071.1"
                     /translation="MKIIIMLFLAVNVFAQEPELVGDPSESSEPTLEAPSEPVAEAIP
                     EPAPVVESASVQSLNFNYAPGNSRLEIGLSSEAQFETTNLENDRQVILELKNTTIARR
                     FARKLDTSSFRSNVSLVSPYQSGENVRVVFQLKEPGSVNFVREGNRLVAVIDNNEGAT
                     SKNNSQASAAPVPDSNDASAATAAPENQNNIAASDSVPTAEPDSNNSGTKESIDSFLD
                     ARSSKRYLGRPITLQLRDAEVHDVFRVIAEASEFNIVLGEDVKGKLTLNLIDVPWDQA
                     LDIVLNSNRLAAERFGSVLRITTIDGLTKEKEAEATAKRAQEAAEPLVVKIFPISYAK
                     PEDMKRILEDFLSKEFAGNSAGGGRRGSIQIEPRTNSLVVRDTPSTMEKVKRILRELD
                     TQTPQVLIEAKFVEVSEAKAREVQGRIFATNREANSEGTGVGFSTTNANWGALFGGST
                     FTSLPTAFAVSPVPGASFGFSPKLGVIPGIGEISAFLSI"
     gene            complement(1464..>1669)
                     /locus_tag="PLH57_10450"
     CDS             complement(1464..>1669)
                     /locus_tag="PLH57_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03072.1"
                     /translation="LLSLPSGKMVIVQEKTILGPRRGSITRIVPGAVFIEERVVNLLG
                     QEEKIETIIEFKEKKNSEQRANL"
BASE COUNT          398 a          423 c          370 g          478 t
ORIGIN      
        1 aatactgaga aatgcactta tttcgcctat gccgggaatc acaccaagct tgggcgaaaa
       61 accgaaactt gcacctggaa ccgggctaac tgcaaaagcg gttggcaaag acgtaaaggt
      121 agaacccccg aagagcgccc cccaattcgc attggtggtc gaaaacccta ctccggttcc
      181 ttcactgttc gcctcacgat tggtcgcaaa tattctaccc tgaacttctc gcgctttagc
      241 ttcgcttact tctacaaatt ttgcttcgat caacacctgg ggagtctggg tgtcaagctc
      301 gcgtaaaatg cgtttaacct tttccatggt ggagggagta tcacgtacga ctaaagaatt
      361 ggtgcgcggt tcgatttgaa tgcttccgcg tctaccgccg ccggccgaat tacctgcgaa
      421 ttctttcgat agaaaatctt ctagaattcg cttcatgtct tcgggcttag cgtaagaaat
      481 tggaaatatt ttcacaacaa gcggctcggc agcttcttgc gcacgttttg cagtagcctc
      541 agcttctttt tccttggtta agccatctat tgtagtgatt cgtagtacgc ttccgaaacg
      601 ttccgccgct aatcgatttg aattcaatac aatatcaagt gcctgatccc aagggacatc
      661 gattagattc aacgtcagct tccctttgac gtcttcaccc aacactatat taaattcact
      721 ggcctcggcg attacccgaa atacgtcgtg aacttcggca tcgcgcaact gtagcgtaat
      781 cgggcggccc aaataccgtt tactagaccg cgcatcgaga aaactatcta tgctttcttt
      841 tgtaccactg ttgttagaat ctggctcggc tgtaggtacg gagtcggacg ccgcaatatt
      901 attttgattt tctggagcgg cagtggcagc cgaggcatca ttgctgtctg gtaccggcgc
      961 agcggacgcc tgactattgt tttttgatgt cgccccctca ttgttatcaa tcaccgccac
     1021 tagtcgatta ccttcgcgta cgaagtttac ggaccctggc tctttaagct gaaacaccac
     1081 acgcacgttc tcacccgatt ggtaggggct taccaaactc acgttacttc taaaggaact
     1141 agtatctaat ttgcgggcaa atcgccgtgc aatggtggta tttttaagtt caagaataac
     1201 ctggcgatcg ttctctaggt tggtagtctc aaactgggcc tcactgctca agcctatttc
     1261 tagccgcgaa ttacctggtg cgtagttaaa gttcaggctt tgcacagatg cggactcaac
     1321 aactggagcc ggttcgggga tggcctccgc tactggttca ctaggtgcct ctaaagtggg
     1381 ttctgaagat tcgctgggat cacctacgag ttccggttct tgggcaaaca cgtttactgc
     1441 taaaaaaagc ataataatta ttttcataaa ttagccctct gttccgaatt tttcttctct
     1501 ttgaactcga tgattgtttc gattttttct tcttggccca acaagttcac tactcgttcc
     1561 tcaataaata cggctccggg tacaattcga gtaattgaac cacgtcgcgg acccaagatg
     1621 gttttttctt gaacgatcac catttttccg cttggcaaag acagcagtg
//
LOCUS       DAOMXO010000572         1668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_43004_length_1668_cov_5.233726, whole genome shotgun sequence.
ACCESSION   DAOMXO010000572 DAOMXO010000000
VERSION     DAOMXO010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43004_length_1668_cov_5.233726"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="PLH57_10455"
     CDS             complement(<1..1545)
                     /locus_tag="PLH57_10455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRK03073.1"
                     /translation="MKVKTFKTDKRFAVVRAASVAKKRGISADQAMDRLLEEIRANPA
                     VQYAEPNYIYKIVGTLKKKQRNALDEPAPTAEPAPAPTAEPAPTAEPAPTAEPAPTAE
                     PAPTATPVPTPAPYVPPVYSPTADELIPNDPDLWKAWGLLNYGQRDSARVEGTPGVDI
                     DAPRAWKVGTGSKRVLVASIDTGVDYTHEDLVENIFVNQKEIDGNGIDDDGNGFVDDV
                     RGWNFFHDNNDPMDDNEHGTHTVGTIGARGDNGIGIAGVAWNVSILPIKFLDKDGYGS
                     LEDALESIKYATLMGAQIMNNSWGGGPFTQSLYEAIADARAKGVLFVAAAGNEKNDND
                     MSAAYPASYDLDNIVSVAATDNKEKLAWFSNWGRNKVHLLAPGFNVYSTVPMNKGKYD
                     SFSGTSMACPHVAGAAALVLSLNPRMTYMDLKNRLMKTSDYVKKLKRRVSSSGRLNVG
                     NAVLNVVPERPLDPLESEWRAIRFKHESEHPYQNSAKTSFAVSATGVSKMRIHFSKVE
                     TEPGYDV"
BASE COUNT          347 a          448 c          422 g          451 t
ORIGIN      
        1 cacatcgtaa cctggctcgg tttcaacttt tgaaaagtga atacgcatct tactcacgcc
       61 agtagcagac accgcgaaac ttgttttagc gctgttctgg tacggatgtt ccgactcgtg
      121 tttgaagcga atggcgcgcc attcactttc cagcggatcc aacggtctct ctggtactac
      181 attcaaaacc gcgtttccta cgttcaagcg accacttgaa ctgacacgac gtttcaattt
      241 cttcacgtag tcggacgtct tcattagtcg gttttttaga tccatgtagg tcatgcgcgg
      301 attcagcgac aacaccaaag cagcggcacc cgctacgtga ggacatgcca tgctggtccc
      361 actgaaggaa tcgtattttc ctttattcat aggaaccgta ctataaacgt tgaaaccggg
      421 tgcgagcaga tgaaccttgt ttcgccccca gttcgaaaac cacgcgagtt tttctttgtt
      481 gtcggtggct gctaccgaaa caatattatc gagatcgtaa gaagctgggt aagcagcgct
      541 catgtcgttg tcattttttt cgttgccagc agctgccaca aacaagacac ctttggcccg
      601 agcatccgcg atcgcttcat agagcgactg agtgaatggc ccgccccccc acgagttatt
      661 catgatctgg gcccccatga gcgtcgcata cttaatcgac tctagcgcat cttccagcga
      721 accgtagccg tctttgtcga ggaacttaat gggcagaata ctaacgttcc atgccactcc
      781 ggcgattccg ataccgttgt caccgcgcgc gccaatagtg ccgacagtgt gggttccgtg
      841 ttcgttgtca tccatcgggt cattgttgtc atgaaagaaa ttccagcctc gaacgtcatc
      901 gacgaatccg tttccatcgt cgtcaattcc gttgccgtcg atttcttttt gattcacgaa
      961 aatattctca actaaatctt cgtgtgtata gtcaacgccg gtatcgattg acgctaccag
     1021 tacgcgttta gaacccgttc ccaccttcca agcgcgcggg gcgtcgatat ctacgccagg
     1081 cgtgccttca acacgagctg agtcccgctg cccgtaattt agcagtcccc aagctttcca
     1141 tagatctgga tcgttcggaa taagctcatc agcggtcgga ctatagaccg gtggcacata
     1201 cggagccggg gttggtactg gcgtagccgt aggtgctggc tctgccgtag gtgctggctc
     1261 tgccgtgggt gctggctctg ccgtgggtgc tggctctgcc gtgggtgctg gcgccggctc
     1321 tgccgttgga gcaggctcgt cgagtgcgtt gcgttgtttc ttttttaggg ttccaacgat
     1381 tttgtagatg taattgggct cggcgtattg aacagcaggg ttcgcacgaa tttcttctaa
     1441 taggcggtcc atcgcttgat cggcactaat tcctcgcttt tttgcaacac ttgcggcgcg
     1501 aaccactgcg aagcgtttat cagttttaaa ggtttttacc ttcattgccg gtgctagagt
     1561 ccgagtcgag ttcacgaatg aacggacgcc gaaacccgat ttgaacttca cgataacttc
     1621 gccagcacgg tagggaaact ttttggtttt gggcgctcgg tccgcgcc
//
LOCUS       DAOMXO010000573         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_43057_length_1667_cov_3.503102, whole genome shotgun sequence.
ACCESSION   DAOMXO010000573 DAOMXO010000000
VERSION     DAOMXO010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43057_length_1667_cov_3.503102"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1061
                     /locus_tag="PLH57_10460"
     CDS             228..1061
                     /locus_tag="PLH57_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03074.1"
                     /translation="MRHVLVLAGLFLSLAGVLSNSTFGAERHDPRYSDDPLTSNLVRI
                     RSENPFIHFTSMRWDQEYKKIVQLTPAEQSRLFTSYAILVAKSRPLPANYRENYQSLD
                     AGELRDLQYLIGLFSYIYSEAFFLKNAPTIYKRLVKNKSFSLHSNWIETLEPKKLKIK
                     SKRQERSSAFAAFGTVGAGLTGATMAAFGFLIGHLDLVMGGGALFSSVLPGSAVAGIS
                     NQLSEEYKHLETLHRFIVNFSNTLALYSGLEWRELSHSNRILALLEDPTTCRALTKSE
                     K"
     gene            1065..>1667
                     /locus_tag="PLH57_10465"
     CDS             1065..>1667
                     /locus_tag="PLH57_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03075.1"
                     /translation="MKLSNLFFLLATILLGMKPTSAHSMNNPTLPTELTCSALLAKSQ
                     LPLGALPYNEITTDSFQPALESAFRECYQRLDLLREESATPTFTNTIEAIEAIDLPLE
                     QAWLVFENYTSLNADPKLDELSQTFTPERKRLSNAIFMDSHLFSRVSAIYDQRDSLGL
                     NTEQTALLVRTYTEFLRNGAKLSDSEKKRVAEIDERLAFLG"
BASE COUNT          441 a          452 c          350 g          424 t
ORIGIN      
        1 tggcggtgct acagctgtaa cgggggcgac ccttgctcgt tcgagcaatg gataacccca
       61 ggtccagagt gcccaccaaa tcgctaaaaa actcatcgtc gttatagttt aaacccgaga
      121 gcgtagttcc acaaatgcca agcccatcga atttgacacg cggcatccag ttcgatagtc
      181 aactaccggt gagcgccagc catgcaagtc tttagggggg gtccaaaatg cgtcacgtct
      241 tggtcctggc cggtttattt ttatcgttag cgggagttct cagtaattca accttcgggg
      301 cagaacgcca cgatcctcgg tactccgacg atcccctgac ttccaatctc gttcgaatac
      361 gttcggaaaa tcccttcatt cacttcacct caatgcgttg ggaccaggaa tacaaaaaga
      421 ttgtccaatt aacgcctgcc gaacagtccc gtctatttac ttcctacgca atactcgtag
      481 cgaaaagtcg accgttaccc gcaaattatc gcgagaacta ccagtctttg gacgcaggtg
      541 aactgcgcga tctacagtac ttgattggac ttttttccta tatttattcc gaggcgtttt
      601 tccttaaaaa cgcgccgaca atttacaaac gtttagtaaa aaacaaatcc ttttcgcttc
      661 actcaaactg gatcgaaaca cttgaaccga aaaaactcaa aataaagtca aagcgccagg
      721 aaaggagttc tgcgttcgcg gcgttcggta ccgtcggagc aggattaacc ggagcaacca
      781 tggcagcgtt tggcttctta attggacatc tcgatctcgt gatgggcggg ggagccctat
      841 tttcatcagt tctccccggg agtgccgttg ccggtatatc gaaccagctc tcagaggaat
      901 acaagcacct cgagacactg caccgcttca tagtaaattt ttcaaatacg cttgcgctct
      961 acagcggcct cgaatggcgt gaactttcgc actccaatcg aatacttgct ctcttagaag
     1021 atcctacaac ttgtcgtgct ttgaccaaaa gtgagaaata aatcatgaaa ctctcgaatc
     1081 ttttttttct tttagctacg attctcctag gtatgaaacc aacatcagcc cactctatga
     1141 acaatccgac actccctact gaactcactt gctccgcgct cctcgcgaaa tctcaactac
     1201 ccctgggagc gttgccttac aacgagatca ctacggacag tttccagccg gcgctcgagt
     1261 ccgcttttcg cgaatgttat cagcgtttag atttacttcg cgaagagtcc gcaaccccta
     1321 cgtttacaaa cacaatcgag gcaatcgaag ctatcgattt gccgctcgaa caagcatggt
     1381 tagtctttga aaactacacc agtctaaacg ccgatcccaa actagacgaa ctttcccaaa
     1441 catttacgcc cgagagaaag cgtctctcca acgcgatttt tatggactcg catctttttt
     1501 cgcgtgtttc cgcgatttac gatcaacgag attctcttgg gttaaacacc gaacaaactg
     1561 cgttgttagt acgcacttat accgagtttc tgcgaaacgg ggccaaactt tcggactccg
     1621 aaaaaaaacg agtcgctgaa attgacgaac gcctggcatt ccttggg
//
LOCUS       DAOMXO010000574         1658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_43395_length_1658_cov_5.061759, whole genome shotgun sequence.
ACCESSION   DAOMXO010000574 DAOMXO010000000
VERSION     DAOMXO010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43395_length_1658_cov_5.061759"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLH57_10470"
     CDS             <1..514
                     /locus_tag="PLH57_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HRK03076.1"
                     /translation="WGKMGQFLACSNYPECKFTAECKKDEKGELILLPREKSNEKCDK
                     CGAAMLIKHGKFGKFLACERYPDCKSTKAITLGIKCPGCKVGEMTARMSRYKRMFYGC
                     NTYPKCNYALWDKPIDKPCPQCQWPILTDKFTKRDGHIIKCPNKECGFKEVIETPEQL
                     AANPKSESAA"
     gene            complement(531..>1658)
                     /locus_tag="PLH57_10475"
     CDS             complement(531..>1658)
                     /locus_tag="PLH57_10475"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRK03077.1"
                     /translation="IVALTPAKSGFHQSIQKKPQKLSLKDKLRFVQRADAAAWSACAD
                     VKQVTVGYLETLRKIQIANSIGILAKDEKQYLTFYTQLVGQRGEQTQTSYEAQGGYIG
                     LELFDQHTPESIAQEAGRRLNSLLSAKPAPAGTMPVVLAAEAGGTMVHEAVGHGLEAD
                     LACNGLSVYKDKIGQRVASPLVSIVDDGTMSGKRGSFAFDDEGEASKHNLLIDKGILK
                     SYMSDRLSHVKFGTPLTGNGRRESYRHKPIVRMTNTLLSSGKDEPESILANTPKGLYV
                     KGLGGGQVNTVTGDFVFAITEGYLIYDGEIGPAVRGATLAGNGPEVLTQVDRVGSDLG
                     FAIGTCGKDGQRAPVTDAQPTIRIPEIVVGGEVPIETYWKK"
BASE COUNT          403 a          386 c          424 g          445 t
ORIGIN      
        1 atggggcaaa atggggcagt ttttggcctg ctccaattat cctgagtgta aatttaccgc
       61 ggaatgtaag aaggacgaaa aaggcgaact tattttgttg ccgcgtgaga agagcaacga
      121 aaagtgcgat aaatgcggtg cagccatgtt aattaagcat ggcaaattcg gaaaatttct
      181 agcgtgtgag cgttatcccg actgtaagag taccaaggcg atcacactag ggattaagtg
      241 tccgggttgt aaagtgggtg aaatgaccgc tcgaatgtcg cgatacaagc ggatgtttta
      301 cggttgcaat acctatccta aatgcaatta cgctttgtgg gacaagccaa tcgacaagcc
      361 gtgtccacag tgtcagtggc caattttgac ggataagttt accaagcgcg acggtcacat
      421 aatcaagtgc ccgaataaag agtgcggctt caaagaagtg atcgaaacgc ctgaacagct
      481 agctgccaat ccaaagtccg aatccgcagc ttaagactct taaacgagag ttattttttc
      541 cagtaggttt cgattggtac ttcaccacca actacgattt ccggaatacg aattgtgggt
      601 tgagcgtcgg tcacgggggc ccgttgccca tctttgccgc aagtgccgat tgcgaaaccc
      661 agatcactgc cgacccgatc tacttgcgta agtacttcgg ggccatttcc tgcgagagta
      721 gcgccacgaa ctgccgggcc aatttcgccg tcataaatta aataaccttc ggtaattgcg
      781 aatacaaaat cgcccgtgac ggtgttgacc tgcccgccac cgagaccctt aacgtaaagc
      841 cccttcgggg tgttcgcgag aatcgattcg ggttcatctt ttcctgacga aagaagcgtg
      901 ttggtcattc gaacaatggg tttgtggcga taactttcgc gtcggccgtt gcccgtcagt
      961 ggggtgccga actttacgtg gctcaaacgg tcgctcatgt agctttttag aatgccttta
     1021 tcgataagaa gattgtgttt tgacgcttca ccctcgtcat caaatgcgaa gcttcctctt
     1081 ttgccactca tcgtcccgtc gtcaacgata ctcacaagtg gactcgcaac tcgttgtccg
     1141 attttgtctt tgtataccga taatccattg caggccaaat ctgcctcgag tccgtgtcct
     1201 acggcttcgt gcaccatggt tccaccagcc tcagcagcta atacgacggg catggttccc
     1261 gcaggggcag gtttggccga aagaagtgag ttaagtctgc ggccggcttc ctgtgcaata
     1321 gattcgggcg tgtgttggtc gaaaagctct aatccaatat aaccgccttg agcttcgtaa
     1381 gaagtttggg tttgttcacc tcgttgtcct acgagctggg tgtagaaagt gaggtactgt
     1441 ttttcatctt tggcgagaat tccgatagaa ttcgctattt gaatttttct caatgtttct
     1501 aagtaaccca cagtgacttg tttgacatct gcgcaagcag accaagcggc tgcgtcggct
     1561 cgttgtacga accgtagttt gtctttgagc gagagttttt gaggtttctt ctggatagat
     1621 tgatgaaaac cgcttttcgc aggggtgagt gcaacaat
//
LOCUS       DAOMXO010000575         1657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_43437_length_1657_cov_5.741573, whole genome shotgun sequence.
ACCESSION   DAOMXO010000575 DAOMXO010000000
VERSION     DAOMXO010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43437_length_1657_cov_5.741573"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..781)
                     /locus_tag="PLH57_10480"
     CDS             complement(5..781)
                     /locus_tag="PLH57_10480"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HRK03078.1"
                     /translation="MHSSIGPLEEALALYVEQSALCQALSSESLVLWDCGLGAGTNAF
                     AAIAAVLGAQAEAPSNHRLQVVSFENDLSGLELALENEKILPYIAQFREPANHVLDRW
                     RKLGPDESLRETISISSNVTWTVFDGDFRTALERAPAPDLIYYDFYSPKVCPDLWTKE
                     VFSLVHSRSNPKCRLFTYSSATRVHVALLDSGFYVGRGQSTGAKNHTTAAAMRMEDLK
                     SPLGPSFLSRLERSHIYSLEERAVLRARLSLHPQWRQSIT"
     gene            812..>1657
                     /locus_tag="PLH57_10485"
     CDS             812..>1657
                     /locus_tag="PLH57_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRK03079.1"
                     /translation="MRTVFVNMNRMRSDHPLLVVVNPHARSSRLNPKIHNRLSKVLSS
                     AGKVELTPSLEALDTLMARQAQSDFQAENRANGTKVPSGSLCFHGGDGSVSRGITSYV
                     RHYRKLAGQDPKILPPILVVRAGTFNMLCNRLGVSGSVLTSVKQWRDGKKSKIETVPT
                     VRVTVDGFEPTYGFVFGWGIGFRVLRNYYSVRAYPSVLDGSVIAAKTIFSGLRPDASD
                     QPLFRGEDTGIKVNGDYVLPQVSVRAIVAGTIERLCLGLRPMPLKTPALGAFHVAATG
                     LPLWKL"
BASE COUNT          385 a          408 c          466 g          398 t
ORIGIN      
        1 tcagctaagt aatagattga cgccactgag ggtgtagaga aagtcgcgcg cgaaggacag
       61 cgcgctcttc taagctatag atgtgggacc tctcaagccg acttagaaaa ctcgggccca
      121 gtggcgactt gagatcttcc attcgcatag cggcggcggt tgtgtggttt ttcgctccgg
      181 tcgattggcc gcgacctaca taaaaaccgg aatctaacag tgcgacgtgt acccgggtgg
      241 ctgaactgta ggtaaataag cggcattttg ggttggaacg cgagtggacg agggagaaca
      301 cttccttggt ccataaatca gggcaaactt tcggtgagta aaaatcataa taaattaaat
      361 cgggagcggg tgctcgctct agggccgttc gaaagtcacc gtcgaatacc gtccacgtga
      421 cgtttgaaga tatcgatatg gtttctcgga gcgactcatc tggaccgagt ttgcgccagc
      481 gatcgagtac atgattagcg ggttcgcgga attgggcaat atagggcaat attttttcgt
      541 tttcgagagc gagctctaga ccagagagat cattttcaaa cgaaacgacc tgtaagcgat
      601 ggttggaggg agcttccgcc tgcgcaccca agacggctgc gattgcggca aatgcgttgg
      661 ttccggcccc taaaccacag tcccacagaa ccagtgactc cgaggacaaa gcttggcaca
      721 gggcagattg ttccacataa agcgccaagg cctcttccag cggtccgata cttgagtgca
      781 tgacctcgcc gctctcgata tggcgtagcg agtgagaacc gtttttgtta acatgaatcg
      841 tatgcgttct gatcatccgc tcctcgtggt ggtgaaccct catgctcggt cttcgcgact
      901 gaatccgaaa atccacaacc ggctctcgaa ggtactatct agcgctggaa aagtcgagct
      961 gacaccgtcg ctcgaagcac tcgatactct catggcgcgc caagcccaga gtgattttca
     1021 ggcggaaaat cgagccaatg gcactaaggt cccaagtggt tctctttgtt ttcatggggg
     1081 cgacggttcg gtgagtcgcg gaataacctc ctacgtacgg cattatcgca aacttgcggg
     1141 gcaggatccc aaaatactac cccctatttt agtggtgcgg gccggtacct ttaacatgtt
     1201 gtgcaatcgc ttaggagttt caggctcggt gctgacttct gtaaaacagt ggcgtgacgg
     1261 taaaaaatcg aaaatcgaaa ctgtgcctac ggttcgggta acggtggacg ggtttgagcc
     1321 aacctatggg ttcgtgtttg gttggggcat agggtttcga gtgttgcgca attattattc
     1381 agtgcgtgcc tatcccagtg tactagacgg ttccgtgatc gcggctaaga ccatattttc
     1441 aggcctgcgg cccgatgcct ctgatcagcc gttgtttcga ggtgaagaca ccggaattaa
     1501 agtgaacggc gattacgtgc tacctcaagt ctcagtccga gcaatcgttg caggcaccat
     1561 tgagcgcctt tgtctggggc ttcggcctat gccgctcaag acacccgcgc tgggagcctt
     1621 tcatgtggcg gcaaccgggt taccactctg gaaactt
//
LOCUS       DAOMXO010000576         1652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_43621_length_1652_cov_6.606763, whole genome shotgun sequence.
ACCESSION   DAOMXO010000576 DAOMXO010000000
VERSION     DAOMXO010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43621_length_1652_cov_6.606763"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..599
                     /gene="rpsF"
                     /locus_tag="PLH57_10490"
     CDS             186..599
                     /gene="rpsF"
                     /locus_tag="PLH57_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRK03080.1"
                     /translation="MKKSTNLTIGYETTYISKPDSGDDALRSFLEKLKGIIKAHGGEV
                     VAVEDWGRRRLAYPIQKETRGVYTHVVYSGNNSLVAELERNLRINEQVIRFLTVKIAD
                     EIDTATFKRKPTANIAATAVPLPAAAVSAAAPITE"
     gene            634..972
                     /gene="rpsR"
                     /locus_tag="PLH57_10495"
     CDS             634..972
                     /gene="rpsR"
                     /locus_tag="PLH57_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRK03081.1"
                     /translation="MRDRDNYRDRDRDNDKDFGGGRDEGKDGEGRGRRSFSRKKVCRF
                     CADNEYIMDYKDARMMQSYMMERGKIVPRRISGTCALHQRKLTTAIKRARNLALVGYI
                     GFSSGGYDAR"
     gene            984..>1652
                     /locus_tag="PLH57_10500"
     CDS             984..>1652
                     /locus_tag="PLH57_10500"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HRK03082.1"
                     /translation="MGEATSLNQNHAHGHHRAFFRLAVLAPFLLGAALHLSVLFLPLS
                     ALPMIFARLRYGRVIGILCGISNLAIVWSLSGRLNAALFFVVGVVLAITLAESLKLKL
                     KLEWAVVASIGAMFLASSLLLLSYSHRNKVNPIKKFDSFVGSMVNQVAKSVEKYKATS
                     SVSNPDLEKFLVDPEMTKKNIIHEFPGAVTITLLMLVLGNLLATLKFNFAEIRQELGL
                     GEDFF"
BASE COUNT          419 a          359 c          429 g          445 t
ORIGIN      
        1 tagggagtcg ggcgagcgag aaacaggcaa tatctcgatg gatgaagtca ttgatttctt
       61 agaagacgag aatttcgtaa cccccgaaat gactttatag tttaatccgg ccggaggttg
      121 tgttaaccct cggcgttcct tgttccgcga attggcgcgg gaccgttagt ccataaggag
      181 gtactatgaa aaagtccaca aatctaacga tcgggtacga aacaacgtac atttctaaac
      241 cggattccgg cgacgacgct cttcgaagtt tcctggaaaa attaaaaggt atcatcaaag
      301 cccatggcgg cgaagttgtt gctgttgaag actggggcag acgccgtctt gcttacccta
      361 ttcagaaaga aacgcgtggg gtttacactc acgtagtata tagcggtaac aactcgcttg
      421 tcgcagagtt ggaacgtaac cttcgcatca atgaacaagt cattcggttt ttaactgtta
      481 agattgcaga cgaaatcgac actgcaacgt ttaagcggaa accgactgcg aatatcgctg
      541 cgacagcggt gccgcttccc gctgcggccg tcagcgcagc tgcccctatt actgaataac
      601 taagttactt tttttaaatt tggagatttt acaatgagag atcgtgacaa ttacagagac
      661 cgtgaccgag acaatgacaa ggattttggc ggcggaagag atgaaggcaa agacggcgaa
      721 ggccgtggtc gccgttcgtt tagtcgcaag aaggtttgtc gtttttgcgc ggataacgaa
      781 tatatcatgg actataaaga cgctcgaatg atgcagtcct acatgatgga acgtggcaaa
      841 atcgttcctc gtcgtatttc cggaacctgt gcgcttcacc aacgtaaatt aaccactgca
      901 atcaagcggg ccagaaacct tgcgttggtt ggttatatcg gttttagcag cggcggttac
      961 gacgctcgtt aagcagcaac cccatgggtg aagctacttc tttaaatcag aaccatgcgc
     1021 atgggcacca ccgggcgttt tttcgcctgg cggtgctcgc gccttttcta ttgggcgcag
     1081 cgctccacct gagtgttttg tttttgccgc tgtcggcttt gccgatgatc tttgcacgtt
     1141 tgcgatacgg gcgcgtgatc ggtattctgt gtggcatttc gaatctcgcc attgtctggt
     1201 cactttccgg gcgattgaat gcggcgctct tttttgtggt tggcgtagta ctggcaatca
     1261 cattggcgga atcactaaag cttaaattga agctggagtg ggcagtggtg gcttcgattg
     1321 gcgcgatgtt tttggcgtcg tccctactgt tgctaagcta ttcccaccgc aacaaagtga
     1381 atccgatcaa aaaatttgat tcgtttgtgg gaagtatggt caatcaagtc gccaagagtg
     1441 ttgaaaaata caaggccaca tcgagcgtat cgaaccctga tctagaaaag tttttagtag
     1501 atccggaaat gaccaaaaaa aatattattc acgagtttcc cggtgcggtt acaatcacgc
     1561 ttttaatgct ggtacttgga aacttattag ccacgctgaa gtttaatttt gcggaaattc
     1621 ggcaagagtt aggactgggt gaggactttt tt
//
LOCUS       DAOMXO010000577         1645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_43886_length_1645_cov_4.105031, whole genome shotgun sequence.
ACCESSION   DAOMXO010000577 DAOMXO010000000
VERSION     DAOMXO010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43886_length_1645_cov_4.105031"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1645)
                     /locus_tag="PLH57_10505"
     CDS             complement(<1..>1645)
                     /locus_tag="PLH57_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03083.1"
                     /translation="HHRMTPGQPVDHVVGTEPFDPILTPLFFIRSLHQGVSNEELGAM
                     YRDVLSKQFTSTNSIAHAKRPSVAHSPELLTKRLIDSASPLAHFLVSQLLYSQFSSLR
                     ALENVLFVTDEHQPAYREVFENVRRLYGEEHYELFISRLDDYLQPIGEPGPKAVDSSS
                     TISNIGSTSHRPSPIRLRHLRLEQIFLSNDRTEARELLTELHRILIDEVSIHPTLKRW
                     AVPRHTVTPPASSVVLFLRHFLMSGPLFQEYVVEDIASRSTITTHSSAAISTRLKRLS
                     ELASNRSPKNTDPEQEWSFLKFWNLLLQIDVNYSHLPSYEDILITAGIKPFDISLEYR
                     TLRDIWEEIYSRATEQQLTAILTTVSNFEQFRQKKDLSHTVRSKLTATATLKKNNLSK
                     PFSEFASPVLITLFTYLRNSLDEMTSHFISAANPFLEQLAQWLEIKYSPRTHDINQIF
                     DEAMEIVRKRIKLNETLPPAGAILQWTSFVHPNKIHLLGKELLDGAFPRLPPRSETLL
                     AMLENFTALHQNKSVGVYLDREILAVFLLALARPDSREKA"
BASE COUNT          419 a          328 c          442 g          456 t
ORIGIN      
        1 ggctttttcg cgggaatcag gacgtgccaa cgcaagcaag aaaactgcca aaatttcgcg
       61 atctaaatag accccgaccg atttattttg atggagcgcc gtgaagtttt ctaacatggc
      121 gaggagggtt tcggagcgcg gtggaagcct ggggaaagcg ccgtcgagca gttccttgcc
      181 gagtaaatga attttgttgg ggtgcacaaa gctcgtccat tgtaaaatcg cccccgccgg
      241 cggaagtgtt tcatttagct ttatgcgttt tcgaacaatt tccatggctt cgtcaaaaat
      301 ctgattgata tcgtgggttc ttggcgaata tttaatttct aaccactgcg ccaattgttc
      361 gagaaaaggg tttgcagcgc taatgaagtg actggtcatt tcgtcgaggc tatttcttaa
      421 ataagtaaac aaagttatta aaaccggtga tgcgaactcg gaaaacggtt tcgatagatt
      481 atttttttta agggttgcgg ttgcggtcaa tttactgcgg acagtgtgag ataaatcttt
      541 cttttgacga aactgttcga agttggacac agtagtgagg atagccgtca gctgttgctc
      601 agtggctcgg gagtaaatct cctcccaaat gtcgcgaaga gttcggtact ccaatgagat
      661 atcaaagggc ttaattcccg cagtgatcaa gatatcttcg taggatggaa ggtgcgaata
      721 gtttacgtcg atctgtagca gtaagttcca aaattttaaa aaggaccatt cttgctcggg
      781 atctgtgttt ttgggcgatc tgttcgaggc cagttccgag agacgtttta acctggtcga
      841 aatagcggca gagctgtgag ttgtgatggt tgagcgacta gcaatatctt caactacgta
      901 ttcctgaaaa agcggtccgg acataagaaa gtgccgcaag aaaagcacta cggaggaggc
      961 cggcggtgta acggtgtgcc gaggtaccgc ccaacgtttt aaggtcgggt ggatactcac
     1021 ttcgtcgatc aaaatacgat gtagctctgt taaaagttct cgggcctcag tgcgatcgtt
     1081 agatagaaat atttgctcta gtcttaagtg gcgtaatcga attggtgatg ggcggtgtga
     1141 cgtgctgcca atgttggaaa ttgtcgaact agaatcgaca gcttttgggc caggctcgcc
     1201 tatcggttga agatagtcgt ctagtcttga gataaacaac tcgtaatgtt cttcaccata
     1261 caagcgacgg acattttcaa atacttctct gtatgctggt tgatgttcgt ctgtgacgaa
     1321 aagcacattt tctagcgctc ttaaacttga aaactggcta tacaacagtt gtgaaactag
     1381 gaaatgcgcg agcggactcg cgctatcgat gaggcgtttg gtaagtagtt cgggcgaatg
     1441 agctacgctg ggtcgttttg cgtgcgcaat tgagttggtg ctagtaaatt gtttgctcag
     1501 gacgtcgcgg tacattgcgc ccaactcttc gttggatact ccctggtgta acgaccttat
     1561 aaaaaatagc ggcgtcaaaa tggggtcaaa cggttcagtt ccaacgacgt ggtcgaccgg
     1621 ctgtcctggc gtcatccgat gatga
//
LOCUS       DAOMXO010000578         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_44087_length_1639_cov_4.356061, whole genome shotgun sequence.
ACCESSION   DAOMXO010000578 DAOMXO010000000
VERSION     DAOMXO010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44087_length_1639_cov_4.356061"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..432)
                     /locus_tag="PLH57_10510"
     CDS             complement(52..432)
                     /locus_tag="PLH57_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03084.1"
                     /translation="MKKNLLSGALFRSTVVACVLLLGLAAFQFSRFYGFHERVRRLIS
                     GDYQGVAKIQVLEGRVKGLLEIINGGSDSVDLDKIYFARLAEIKKDLDTLKSGMSDRA
                     LGDIDRILERAGTLKRDAPNQTAF"
     gene            complement(429..1430)
                     /locus_tag="PLH57_10515"
     CDS             complement(429..1430)
                     /locus_tag="PLH57_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03085.1"
                     /translation="MNDLYSIASELVTERKMLLEAVKRLERSVRIVDETPAHQSVAAL
                     HESIGRVGGLLRAILQLSEMNQERDFGAVARAIESRLRDHGKKISKNVDIVFADPTAV
                     KADAAILDKITTPLIELFICTLDYGVETERERLLRGKKKNTVFRINAVPCSAGILMTV
                     NFDGNGVIPPLSLQQSRQLGAAGIRLEFQGTPGVESSWRVIFSSSQKTIKCIRAVVDG
                     VECAIPLWSSFGIKKNVTVTRGTLYSLTRDWNLRELPQGSKVTYLITMGAGSAKKNLV
                     LDDMHGADDVLIRELPPALLAGGKVLGLAVWSDTSANMVRLLPVIDPNWIAHSRSGE"
     gene            complement(1427..>1639)
                     /locus_tag="PLH57_10520"
     CDS             complement(1427..>1639)
                     /locus_tag="PLH57_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRK03086.1"
                     /translation="KKMVSEAIKDLPEKQRLVLSLYYYEDLNLKEIGRVLEVTESRVS
                     QLHTQAIIRLKSKLKNHFDDFITMLA"
BASE COUNT          400 a          448 c          361 g          430 t
ORIGIN      
        1 aggaacgccc gaaccgcctc gcgggcgccc tcataggctt ccgtggccgc attagaacgc
       61 agtttgattt ggcgcatcac gcttaagtgt gcccgcccgt tctagaatgc ggtcaatatc
      121 gcccaatgcg cggtcgctca tgccagactt caaggtatct aagtcttttt taatctctgc
      181 gagtcgcgcg aaataaatct tatcgaggtc aacggagtca gacccaccat tgattatttc
      241 tagaaggccc ttcactcgcc cctcgagaac ttgaatctta gctacgccct gatagtctcc
      301 tgaaattaat cttcgaacac gttcgtgaaa tccataaaaa cgagaaaact ggaacgccgc
      361 caaccccaga agcaaaacgc acgcaacaac cgtactgcga aatagtgctc cactgaggag
      421 gttcttcttc attcaccgct ccttgagtga gcgatccaat ttgggtcgat tacaggcagc
      481 aatctgacca tattcgcact tgtgtcgctc caaaccgcga gcccgaggac tttgccgcca
      541 gctaacaatg ccggaggtag ttctctaatc aatacatcgt cagctccatg catgtcgtcg
      601 agtaccaggt tctttttggc actgccggct cccattgtaa tgagataggt caccttagac
      661 ccttgaggaa gttcccgcag attccagtcc cgagttaagg agtaaagtgt tcctcgagta
      721 actgtcacat ttttcttgat gccaaaacta gaccacaacg gaatagcgca ctctactccg
      781 tcaactaccg cacgaatgca cttgatcgtt ttctgactac tggaaaagat gactctccag
      841 gaagactcca ctccaggggt gccttgaaac tctagtctta ttcccgcggc gcctaactgc
      901 cgactttgtt gcagcgaaag tggtggaatc acgccgtttc cgtcgaaatt caccgtcatc
      961 aaaataccgg cgctgcaggg cacggcattt attcgaaaga cggtattttt cttttttcca
     1021 cgaagcagtc gctctcgttc ggtctcaacg ccataatcta gcgtacaaat aaaaagttcg
     1081 atcagtgggg tggttatttt atcaagtatt gcggcgtcag cttttactgc ggttggatct
     1141 gcaaaaacga tgtcgacgtt tttacttatt ttttttccgt gatcacgtaa gcgactctcg
     1201 attgctcgcg caacggcgcc aaaatcgcgt tcctggttca tttcgctcag ctggagaatc
     1261 gcccgcaaca acccgcctac ccgaccgatt gattcgtgaa gtgcggccac tgactgatgc
     1321 gcaggcgtct catcgactat ccgcacagac cgttccaggc gcttcaccgc ttccaacaac
     1381 attttgcgct ctgtaaccag ttcactggca attgaataca aatcgttcat gcaagcatcg
     1441 ttataaaatc gtcaaaatga tttttgagtt tagatttcag gcgtataatc gcctgtgtgt
     1501 gcagctgcga cactcgggac tcggtaacct ctaacacccg cccaatctcc ttgaggttta
     1561 ggtcttcgta atagtaaagc gacagcacta accgctgttt ttcagggaga tctttaatgg
     1621 cctccgagac cattttttt
//
LOCUS       DAOMXO010000579         1636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_44227_length_1636_cov_3.595193, whole genome shotgun sequence.
ACCESSION   DAOMXO010000579 DAOMXO010000000
VERSION     DAOMXO010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44227_length_1636_cov_3.595193"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLH57_10525"
     CDS             complement(<1..952)
                     /locus_tag="PLH57_10525"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRK03087.1"
                     /translation="MSHRRILKLQKNILLMIRAEVRKTLGEQPRELRSYYEAYKKEFR
                     GAAEYSNRPALIAAAQKADIVLVGDYHTFSQSQRSALRLLTDLWNLDDPTEPNKRGLA
                     LGLELVRTEDQKYLDQFLEDAISENELLKKIDYSKKWGFPWENFRPLVLFAKERGLPV
                     LALNTGTSELTARDQHAAEVISQFALANPNRRLVVLYGDLHLASGHIPRKIKTELARA
                     KSKRKILTVFQNSESLYWRLASQGREHTVDVLKLSSDRYCIISAAPWVKLQSYLQWAE
                     ASQLIDEGEGISFHELTTQNLKHLAEDAAANTWLFGSRRML"
     gene            complement(953..>1636)
                     /locus_tag="PLH57_10530"
     CDS             complement(953..>1636)
                     /locus_tag="PLH57_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03088.1"
                     /translation="GAIVRRLQWLRRIAEVYQNSGDPELLVSLARTEKLFASTLDRAE
                     KVSGLGIRKPYPLTELLDDTPVESFLARFYDELCAFEAHLFDLLHAHHQRVGETEAQI
                     RIEECINICALWGREIAGEKLLIPNDERYDSYSVFEGLYQFLEGGDLQWKPLLPRRLT
                     ENEVHYELRFCPHARGHSVSSNAEAKTLACRFESVVFEHFAKFLAHNATFSRTRGLDH
                     CTDRIFLNN"
BASE COUNT          442 a          382 c          376 g          436 t
ORIGIN      
        1 gcagcatgcg acggctgccg aaaagccagg tgtttgccgc cgcatcttcc gccaaatgtt
       61 taaggttttg agtagtgagt tcgtgaaaac taatgccttc accttcgtca attaattggc
      121 tggcttctgc ccactgtaaa tatgattgaa gtttaaccca gggagctgcg ctgattatgc
      181 aatatcgatc ggaacttaat ttaagaacgt cgaccgtgtg ttcgcgcccc tgtgaggcaa
      241 ggcgccaata caaactttca ctgttctgaa aaactgtgag aatttttcgt ttagacttag
      301 cacgcgctaa ttctgttttt atttttcgcg gaatatgacc actagccaaa tgaaggtccc
      361 catacaagac cactagcctt cggttcggat tggccaatgc aaattgagaa ataacctctg
      421 cggcgtgttg atctcgcgca gtaagctccg aagtaccggt gttgagtgcc aacaccggga
      481 gtccgcgctc ctttgcgaaa agaactagcg gcctaaagtt ttcccaggga aaaccccact
      541 tttttgagta atcaatcttc tttagcaact cgttttctga aattgcgtct tccagaaact
      601 gatccaaata tttttgatct tccgttcgca ccagttctaa accaagagcc agaccgcgtt
      661 tgttgggttc ggtagggtcg tcgagattcc aaagatcggt taaaagtcgt agggccgatc
      721 tctgtgactg ggaaaacgtg tgataatcgc caaccaaaac gatgtcggct ttttgggcgg
      781 ctgcgatgag ggccggacga ttagaatatt cggcagcgcc acggaattct tttttgtaag
      841 cttcgtaata actacgcagt tcccgtggtt gttcgccaag ggttttacga acctcagctc
      901 gaatcattag gagaatgttt ttttgtaatt taagtattct ccgatggctc atttagttat
      961 ttaaaaaaat ccgatcagtg caatgatcta agcctcgagt gcgtgaaaaa gtagcattgt
     1021 gagctaaaaa tttagcgaaa tgttcaaaaa ctacactctc gaatctacaa gctagagttt
     1081 tggcttccgc attactcgat acagaatgac ctctagcatg tggacaaaat cgcaactcat
     1141 agtgaacttc attttcagtt aaacggcgcg gtagtaaagg cttccactgc aaatctccac
     1201 cttccagaaa ctgatagagc ccttcgaaaa cgctatagga gtcgtaccgc tcatcatttg
     1261 gaattaagag cttttcgccc gcaatctctc gcccccaaag cgcgcatata ttgatacact
     1321 cctcgatacg aatttgagcc tcagtctcgc cgacgcgctg gtgatgcgca tgcaaaagat
     1381 caaaaaggtg agcttcgaaa gcgcaaagct cgtcgtaaaa gcgagcaaga aacgattcga
     1441 ctggtgtatc gtctaacaat tcagtgagcg gatagggctt acgaatgccg aggcccgaaa
     1501 ccttttcggc acgatctaag gtagatgcaa aaagtttttc ggttctggct agcgaaacca
     1561 gaagttcagg gtctccgcta ttttggtaaa cctccgcaat acgacgcaac cactgtaggc
     1621 ggcgaacaat ggctcc
//
LOCUS       DAOMXO010000580         1634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_44293_length_1634_cov_4.556681, whole genome shotgun sequence.
ACCESSION   DAOMXO010000580 DAOMXO010000000
VERSION     DAOMXO010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44293_length_1634_cov_4.556681"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..941
                     /locus_tag="PLH57_10535"
     CDS             174..941
                     /locus_tag="PLH57_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRK03089.1"
                     /translation="MRGSVVAIGTELTSGQIINSNAAKLSAALTSLGVDSVFHITVPD
                     DHGAILHALRSAELVSELIFVTGGLGPTTDDFTRDVVAAWAGLPLYFDDSAWARLIKK
                     LELRGRVASNSQKQQCSFPQGAMILENPKGTAEAFFLRVRDRRVWVLPGPPLEIEAIW
                     DAGVRKMIEQLIPDGPKYELLSWICKDKAESEIGDLVESIVRGTGLRTGYRCFKPYVE
                     VKIWCLRSDLPKHSAQFDRINDALKNWLDPKPTTSSD"
     gene            complement(1012..>1634)
                     /locus_tag="PLH57_10540"
     CDS             complement(1012..>1634)
                     /locus_tag="PLH57_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03090.1"
                     /translation="LMPYANLPYFLDAMKDLKESVYKTKKPNSAERYLKEIRLYAKKK
                     EKDTELLCDSNAVVKDSKEVERLTFTRDISSTTFIYSRSYWQSREELEYGGEATKREL
                     FKPERIEMKYRPNVSKDANAEIKALTIRGTLYKNSGSEHPFFKCGEVRKKKVYVEPKH
                     VDDNYDRKLTVGTLFPAFDDDGEVLPGEPVLPPSDGNYVCPQKRAK"
BASE COUNT          397 a          365 c          401 g          471 t
ORIGIN      
        1 atgattccat tttttgaaat ttacaacatt acctacgttt ttgcgaaagc gctaaagaac
       61 aggaatgttt ccattgccgt tgagggactt aggggtctca aagacggctt tcgttttttt
      121 tcacccagcg cgatccggta tcgcagaaca tccgaaggga gtaacgatcg atcatgagag
      181 ggagtgttgt cgcgatcgga acagaactta cgtcgggtca gattattaac tccaatgccg
      241 caaaactttc tgcggcgctt acttcgctgg gtgtagattc cgtttttcac attaccgtgc
      301 ctgacgacca tggtgcgatt ttgcatgcgt tgcgatcggc ggaactagtt agtgagttga
      361 ttttcgttac gggtggttta gggccaacga ccgacgattt cacccgagat gttgtggcag
      421 cgtgggcggg acttccgctt tattttgatg attcggcgtg ggctcgtcta attaaaaaac
      481 tggaacttag aggtcgcgtt gcatccaaca gtcaaaaaca gcaatgttcc tttccgcaag
      541 gcgccatgat cttagaaaac ccgaaaggga cagccgaggc ttttttttta cgagtgcgtg
      601 atcgtcgggt gtgggtgctc cctgggccac cgttggaaat cgaggcaatt tgggacgccg
      661 gggtgcgaaa gatgatcgag caattgatcc ccgatgggcc aaagtacgag ttactttcct
      721 ggatttgcaa agacaaggca gaatccgaaa ttggtgatct cgttgaatcg atagttcgcg
      781 gtactgggct tcgaaccggc tatcgctgct ttaagcccta tgttgaagta aagatttggt
      841 gtcttcgttc agacctgcct aaacattctg cacaattcga ccgcataaat gacgctctaa
      901 agaattggct cgaccctaaa ccgaccactt cttccgatta aacaaaaaag ccgcgggcta
      961 ttaacccgcg gctctctaga tattgtcgaa ctagttcttt atagcgatca attacttagc
     1021 acgcttctga gggcaaacat aattgccatc ggaaggcgga agtaccggtt caccagggag
     1081 aacctctcca tcgtcgtcaa acgctggaaa cagtgtgccc acggtgagtt tacgatcgta
     1141 attgtcgtcg acgtgtttag gttctacgta aacttttttc ttacgtacct cgccgcactt
     1201 gaaaaacgga tgttcactgc cagagttttt gtacagagtg cctctaatcg tgagcgcttt
     1261 gatctctgcg ttggcatctt tagaaacgtt tggacgatat ttcatctcaa tacgctctgg
     1321 tttaaagagc tcacgtttag tggcttcgcc accgtactcg agctcttcac gtgactgcca
     1381 gtagctacga ctgtaaataa acgtagtaga agagatatca cgagtgaacg ttaatcgttc
     1441 aacctctttg gaatctttta ccacggcatt tgagtcacaa agcagttcag tgtctttttc
     1501 ttttttcttg gcgtacagac ggatttcttt cagatagcgt tcagccgaat ttggtttttt
     1561 tgtcttgtaa acgctttctt tgagatcctt catggcgtcc aagaagtatg gaaggtttgc
     1621 gtaaggcatc aaat
//
LOCUS       DAOMXO010000581         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_44359_length_1632_cov_4.287254, whole genome shotgun sequence.
ACCESSION   DAOMXO010000581 DAOMXO010000000
VERSION     DAOMXO010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44359_length_1632_cov_4.287254"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PLH57_10545"
     CDS             <1..963
                     /locus_tag="PLH57_10545"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRK03091.1"
                     /translation="KRDVPFDAEEVSKWLPLDQYIGGVEHATMHCLYFRFFHRVLQDL
                     GFLPKSVGREPAKNLLNQGIVYKDGAKMSKSKGNVVDPNSLIAKFGADTTRMFSLFAA
                     PPEKTLEWSDQGVEGSFRFLGRVWRLIQPLAAELKGVPGYSGTHREIVTPEIKKLRAK
                     AHLTIKKVTEDIDRDFHFNAAIASIMELVNEAYGVPKEKLHDVSARSVLRETCEIILR
                     LLNPFAPHTTEELWERIGGAGLLVDQPWLSFDPHAVEAETLTIVVQVNGKLRANLFVA
                     PGTDQKELERLASSDAKVQNFIAGKTVVKTIVVPGKLVNFVVRD"
     gene            969..1583
                     /gene="lptE"
                     /locus_tag="PLH57_10550"
     CDS             969..1583
                     /gene="lptE"
                     /locus_tag="PLH57_10550"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRK03092.1"
                     /translation="MLGLFRRIQLFWVLVVVLGCLQAGCGYNWQGTSNLWAKEKIEKI
                     YIRTLDNKTLTAGVETFFTSAIIREFSKGRRLTITTSEGDADAVLWGTIESVDNTGSP
                     GADIELGQVSTESGAGQLDRVPIFSEYVATAFLNINLTRRRDGEVVWNGGFGHPGYQY
                     PGSARFGLPGTTSSLINDSQFKLALEEVAKTIATDVHDTLLERF"
BASE COUNT          408 a          369 c          443 g          412 t
ORIGIN      
        1 aaacgggatg ttccgtttga tgcggaagag gtctccaagt ggttaccgct agaccagtac
       61 attggtggag tcgaacatgc gaccatgcac tgcttgtatt ttcggttttt tcaccgcgtg
      121 ctgcaggact tgggattttt gccgaaatcg gtagggcgtg aacctgcgaa gaatctcctc
      181 aatcaaggca ttgtttacaa agacggcgcc aagatgagta aaagcaaggg aaacgttgtt
      241 gatcccaatt ctttgatcgc gaaatttggt gccgacacga cccgcatgtt ttcattgttc
      301 gcggctcctc cagaaaaaac tttggaatgg agcgatcagg gtgtcgaagg gtcctttcgc
      361 tttttaggtc gcgtatggcg cttgatccag ccgctggctg ctgagctcaa gggtgtgccc
      421 gggtacagcg gcacgcatcg cgagatcgtc accccggaaa taaaaaagct tcgggccaag
      481 gctcatttaa ccattaaaaa agtaacggaa gatatcgacc gagattttca ttttaacgcc
      541 gcaatcgcgt cgatcatgga actcgtcaac gaagcttacg gcgtacctaa agagaagttg
      601 catgatgttt cggcaagaag cgtattgcgc gaaacttgcg agattatttt gcgactttta
      661 aatccgttcg cgcctcacac gaccgaggaa ctgtgggaac gcatcggcgg ggcaggtttg
      721 ttggtagatc aaccttggtt gagcttcgat ccccatgcgg tagaggcgga aaccttgacc
      781 atcgtggtgc aggtgaacgg aaaacttcga gctaatcttt tcgtggcccc gggcaccgat
      841 caaaaggaac tcgaacggtt ggcttcgagc gatgcaaaag tgcagaactt tattgcgggt
      901 aaaacggtcg tgaaaacgat tgtcgtacca ggtaaacttg taaattttgt agtaagggac
      961 taacgcgaat gctggggttg ttccgaagaa ttcagttgtt ctgggtattg gtagtggtgc
     1021 tcggctgctt gcaagccggc tgtggttaca attggcaggg cacttcgaat ctttgggcta
     1081 aagaaaaaat cgaaaaaatt tatattcgaa cgctcgataa caaaacactt accgccggag
     1141 tggaaacttt tttcactagc gccattatta gggaattttc caaaggtaga cgactgacta
     1201 ttaccacctc agagggtgac gccgatgcgg tcctttgggg caccattgag tctgtcgata
     1261 acaccggttc gcccggtgcc gacatcgaat tgggtcaggt gtctaccgaa tcgggcgcag
     1321 ggcaacttga ccgcgttccg attttttcag agtatgttgc caccgcgttt ttaaacatca
     1381 atcttacgcg gcgacgcgat ggcgaagtgg tgtggaatgg cggtttcggc catccgggct
     1441 atcagtaccc ggggagtgca cgttttggac ttccaggaac aacctcatcg ctgatcaatg
     1501 attcgcaatt taagcttgct ctcgaagagg ttgccaaaac gattgcgacc gacgttcacg
     1561 acactttgtt ggagcgattt tgaaaccatc gatcaagacg aatgatcttt ctccgaaaca
     1621 gattcttgcc aa
//
LOCUS       DAOMXO010000582         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_44553_length_1627_cov_3.862595, whole genome shotgun sequence.
ACCESSION   DAOMXO010000582 DAOMXO010000000
VERSION     DAOMXO010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44553_length_1627_cov_3.862595"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..>1627
                     /locus_tag="PLH57_10555"
     CDS             408..>1627
                     /locus_tag="PLH57_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03093.1"
                     /translation="MAHLSRGRFSRAVSSLMLAVFTVSSVFSVFPTSIHADFINEGRR
                     ALVEDEYQAGNESGKDSEALYQEAMKALGDSVYATYSDMLKHCESDAVDHAENCYFDL
                     AVDAHGNVVSKNAIATNEIAKNPSKYEGLHLRECGTHPDNPKQKMLCFNFVDIATFFN
                     KKNLDIKDAKKKYSKLFNLYEALVRRYAANIAFRLYSPKETDSQSCFDAYSPLELNRY
                     LDDHAADHFFMLPDGSRPFLGSDGKPIPCKRACRLVPPVLKKHSPNGDKFVCFEPQYE
                     WTQFEMQSIPFVEGCPEWPTNECTANTLYQYQYNKGSKGTYRDKDSSGTWANPDLNYR
                     GTNADGSTVLPNLANVLFSEDFKDLTPLVDSLFSAGFTDQANQYFAELMIEGYLEEAS
                     LNALGDKNAAVADA"
BASE COUNT          454 a          398 c          372 g          403 t
ORIGIN      
        1 gttccagttc tgagacttcg gcttgaaaat gaaaatactc cgcttccacg gccagattga
       61 tttcgagttc tggtttaagc gaacggatca aggtggactt tccgacctgg cgcggcccga
      121 gcagaagaat gctcttgcga gatccacgaa gcgaacccga taacattctg ttatacatgc
      181 ggttattttt acatacaaaa caaccgctat caactttttt cgtggcggtg cccctgtaac
      241 tcagtcgttt gctctcaatg catctcaagt ttcccctgcg cacggccgaa aaagctaaag
      301 agacgatttg agagactagc ttgactgtat tcactggtgt gtgccagtga cacagttgtt
      361 cgagccctcg cagtctgtaa aatagagaag ttgggggatc accgttaatg gctcatttat
      421 cgcgtggccg tttttctcga gcagtcagtt cgctgatgct tgccgtgttt acggtgagct
      481 cggtgttttc ggtttttcca acatcaatac acgcagattt tatcaacgag ggccgtaggg
      541 cgctcgtcga ggacgaatac caagctggca atgaatctgg aaaagattct gaagcactct
      601 accaagaagc aatgaaagca ttaggagatt cggtctacgc cacctactct gacatgctga
      661 aacactgcga atcagatgcg gtagatcacg ctgaaaattg ttatttcgat ctcgcagtcg
      721 acgctcacgg aaatgtcgta tcgaagaacg cgatcgcgac taatgaaata gcgaaaaacc
      781 catcgaaata cgaaggactt caccttcggg agtgcggcac acaccctgac aatcccaaac
      841 agaagatgct gtgtttcaac tttgtggaca ttgccacgtt cttcaataag aaaaatctcg
      901 atattaaaga cgcaaagaaa aaatacagca agctgtttaa tttgtatgag gcactcgtca
      961 ggcgctacgc tgcgaatatt gcctttcggc tatacagccc taaggaaacc gactctcaat
     1021 cgtgctttga cgcgtactca ccactcgaat taaatcgcta tttagatgat catgccgccg
     1081 atcatttttt catgctgccg gatggaagcc gtccatttct gggatcggac ggtaaaccaa
     1141 ttccctgcaa aagagcgtgc cgtttggtcc cgcccgtact caaaaaacat tctccgaacg
     1201 gggacaaatt cgtttgcttc gaaccacaat acgagtggac acaatttgaa atgcaatcga
     1261 tcccttttgt cgaagggtgc cctgaatggc ctacgaatga atgcacagca aacacacttt
     1321 accagtacca atataacaaa ggctcgaaag ggacttaccg cgataaagat agttctggca
     1381 catgggcaaa cccagattta aactaccgag gcactaacgc cgacggctcg acggtgctcc
     1441 cgaatctcgc aaacgtcctt ttttccgagg acttcaaaga tttaactccg ttggtagaca
     1501 gcttatttag tgctggattt accgaccaag ccaaccagta ttttgccgaa ctcatgatag
     1561 agggttacct tgaagaggca tctctaaacg cattgggcga caaaaatgcc gcagtagccg
     1621 atgcaat
//
LOCUS       DAOMXO010000583         1624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_44675_length_1624_cov_4.365201, whole genome shotgun sequence.
ACCESSION   DAOMXO010000583 DAOMXO010000000
VERSION     DAOMXO010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44675_length_1624_cov_4.365201"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1494)
                     /gene="clpX"
                     /locus_tag="PLH57_10560"
     CDS             complement(226..1494)
                     /gene="clpX"
                     /locus_tag="PLH57_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRK03094.1"
                     /translation="MEKKLGDGFGNLCCSFCGKNQREVKKLIAGPTVYICDECIELCN
                     DIIAEEGQKEAAKTTTERIPKPNEIKTVLDEYVIGQERAKKILSVAVYNHYKRIQHKP
                     TSRDKDAVELSKSNILLIGPTGSGKTLLAQTLARLLNVPFAMADATTLTEAGYVGEDV
                     ENIILSLLQSCDFNVERAQKGIVYVDEVDKIARKGDNPSITRDVSGEGVQQALLKILE
                     GTIASVPPKGGRKHPQQEYIQVDTTNILFICGGAFVGVDRVIASRQGKRSLGLGAKIS
                     SKENDTLSATIAKIEPEDLVRFGLIPEFIGRLPVVATLEELDETALVEILKKPKNALT
                     KQYQKLFQYDGVELEFEEEAISAIAREAIKRNTGARGLRAIMESCMLDVMYDIPNKAD
                     VKKVIVTRNCIEKGERPIVMMADGGKAESA"
     gene            complement(1503..>1624)
                     /locus_tag="PLH57_10565"
     CDS             complement(1503..>1624)
                     /locus_tag="PLH57_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRK03095.1"
                     /translation="TAQKTDQIQIDTERDNYMSSEEAMKYGLIDRVITNRESV"
BASE COUNT          404 a          406 c          321 g          493 t
ORIGIN      
        1 ccttaattta acttgatcat agtgccggat ctgtgtcaaa cgctgcaatc cgccaaacac
       61 tcaaaatcac tgatgattgc gcagtatttg cccaagacta tggcacgaat tttgagacct
      121 tatctcgacc gcgaaatatc tgcatggaaa cgtgtaaaaa aaccagtgca aaaagacacc
      181 ggtcccgaaa cgaactttac atttttttac tttcgagttt cgaaactagg ccgactcggc
      241 cttgccaccg tcggccatca tcacaattgg gcgttcacct ttttcaatac aattgcgtgt
      301 cacgatgact ttcttaacat ctgctttatt cggaatgtcg tacatcacat cgagcatgca
      361 actttccatt attgcgcgca atccacgggc tccggtattt cgttttatcg cctcgcgtgc
      421 aatcgctgaa attgcttctt cttcaaactc caattcaacg ccgtcatact gaaacaattt
      481 ttggtactgt ttcgtcagtg cgttctttgg ttttttcaga atttccacaa gagccgtttc
      541 gtctaactct tcgagcgtgg caaccactgg tagacggcca ataaattctg ggatcaaacc
      601 aaaacgcacc aaatcctctg gttcaatttt cgcgatagtc gcagacaatg tgtcgttttc
      661 tttgcttgag atttttgcac ccaaaccaag actacgttta ccctggcgtg aggcaatgac
      721 tcgatctaca ccaacaaaag ctccaccgca gatgaacaag atgtttgtag tatcaacttg
      781 aatgtactct tgttgagggt gttttcgtcc accttttgga ggcaccgaag caatagttcc
      841 ttctagaatt ttcaacagtg cctgttgaac tccctcacca ctaacatcac gcgtgatcga
      901 gggattatcg cccttgcgcg cgatcttatc gacctcatct acgtaaacga tacccttttg
      961 ggcacgttcc acgttgaaat cacagctttg cagtaggctc aaaataatgt tttcaacatc
     1021 ttcacctaca taaccggcct cagtaagagt cgtggcgtca gccatcgcaa aaggaacatt
     1081 caacaaacgc gccagtgttt gggccagcag tgttttgccg gagccggtcg gaccaatcaa
     1141 caaaatgttg gacttcgaca attcgactgc gtctttatcg cgcgaagtag gcttgtgctg
     1201 aatacgtttg tagtggttgt aaactgctac agaaagtatc ttttttgcgc gctcttgccc
     1261 gattacgtat tcgtcaagta ccgttttgat ttcgtttggt ttcggaatcc gctcggtcgt
     1321 agttttcgca gcctcttttt gaccttcttc agcgatgatg tcgttacaga gctcaataca
     1381 ctcgtcgcaa atataaacag ttggtcctgc gattagtttt tttacttctc tttgattttt
     1441 cccacaaaaa gagcagcaga gattaccaaa cccgtcaccc agtttctttt ccatggttct
     1501 ccttaaacac tttcccgatt ggtaattaca cggtcgatta agccatactt catggcttct
     1561 tcggaggaca tgtaattatc gcgctcagtg tcaatttgaa tttgatctgt tttctgagcc
     1621 gtat
//
LOCUS       DAOMXO010000584         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_44928_length_1618_cov_3.955214, whole genome shotgun sequence.
ACCESSION   DAOMXO010000584 DAOMXO010000000
VERSION     DAOMXO010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44928_length_1618_cov_3.955214"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1110
                     /locus_tag="PLH57_10570"
     CDS             <1..>1110
                     /locus_tag="PLH57_10570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRK03096.1"
                     /translation="IAALSIWVIERGSLLRAGIGDEDDGVAESFVVPEEEGEPEVLTA
                     EPVAFVRPTFAGKRLHSLAVEAGFRYRSSDVEHVVGSKSSPQIKKSEVVFPLSQLLFQ
                     GGGKVWKAPDGKLYQPAHEYVPVLKSLDAAKIIYQGQAALEQLALLMMHAEKAAIVFH
                     NDLAGVTLASKPLRLVGMHIGKKSDKARSVSYEFANDEARITSDEMAKLSEIGRLSTN
                     FVWIGNKIFRLSTSLAQLARLANRSGVAGPESSPRVKTPTGFIDLEDNNQQPLHPLAA
                     YRLSLEVGVEDFSVDADWKEFHEWRKNFERKDIPELPNVEYGFDLREYQKNGLSWLWS
                     LSHRGLAALLADDMGLGKTHQVMAFLSSMYEGKDSP"
     assembly_gap    1111..1120
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1121..>1618
                     /locus_tag="PLH57_10575"
     CDS             <1121..>1618
                     /locus_tag="PLH57_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRK03097.1"
                     /translation="PEHPSLVIAPTSVVASWSHKLERYKTGLKWSVFHGKGRVMPCKD
                     TKLVLTTYGILQKESALRERPWHVVILDEAQAIKNATTISARASRVLKCKFRLAMTGT
                     PVENQASDLWSIMEFLLPGYLGSLPRFKRLYGYGREIPTKEQATTLKRLVSPFLLRRT
                     KSQVLT"
BASE COUNT          396 a          389 c          437 g          386 t
ORIGIN      
        1 catagcggcc cttagtattt gggtgatcga gcgaggcagt ttgctgcgcg ccggaattgg
       61 cgatgaggac gacggcgtag ccgagtcttt cgtagtaccc gaagaagagg gtgaacccga
      121 agttttaacg gctgagcctg tggcatttgt gcggcctacc tttgccggaa aacgcctgca
      181 ttctttggcc gtagaggcag gctttcgtta tcgtagttcc gacgttgaac acgtcgtggg
      241 aagcaagagc tcgcctcaaa taaagaaaag tgaagtggtg tttccgctct cgcaactgct
      301 atttcagggc ggcggcaaag tgtggaaggc ccccgacggc aaactatacc aacccgctca
      361 cgaatatgtg ccagttttaa agtcgctaga tgcggcgaag attatttatc agggccaagc
      421 agcgctcgaa cagctcgcgc ttttaatgat gcacgcagag aaggcagcta tcgtttttca
      481 taacgatcta gcgggcgtaa ctttggcatc taaaccactt cgattagtgg gcatgcatat
      541 tggcaaaaaa tccgacaagg cccgctcggt gtcctacgaa tttgctaacg acgaagctcg
      601 aatcaccagc gatgaaatgg cgaaactttc ggaaatcggg cgcctctcta ccaacttcgt
      661 ttggatcggt aataaaattt ttcgactctc aacgtcgctc gctcagcttg cacgcttggc
      721 taatcgcagc ggtgtggcgg gcccagagtc gtcgccgcgg gtaaagactc ctacggggtt
      781 tattgatctc gaagacaaca accaacaacc actgcacccg ctagcggcct atcgattgag
      841 tctcgaggtc ggagttgaag atttttcggt cgatgcagat tggaaagagt ttcacgagtg
      901 gcgtaagaac tttgaacgca aagacattcc agaattgcca aacgtggaat acggattcga
      961 tctgcgcgaa tatcaaaaaa atggtctgag ttggttgtgg agtttgtcgc atcggggact
     1021 cgccgcgctt ttggccgacg acatggggct tggtaaaacc caccaagtaa tggcttttct
     1081 ttctagtatg tacgaaggca aagacagccc nnnnnnnnnn cccagaacac ccgagtttgg
     1141 tgattgctcc tacgtcggtc gtagcgtctt ggagccacaa actcgaacgt tataaaacgg
     1201 ggctcaagtg gagcgtgttt catggcaagg gtcgagtaat gccatgtaaa gacactaaac
     1261 tagtactaac gacctacgga attttacaaa aagagtctgc actgcgcgag cgcccctggc
     1321 acgtggtaat cctcgatgaa gctcaggcca ttaaaaatgc gaccaccatt agcgcgcgcg
     1381 cttcgcgtgt tctgaagtgc aagtttcgat tggcgatgac cggtacgccg gtagagaatc
     1441 aggcttccga cctttggtcg atcatggagt ttttgctccc tggttatttg ggttcactgc
     1501 cgcgttttaa gcgtctttac ggatatggcc gtgagattcc gaccaaagaa caagcgacca
     1561 cacttaagcg acttgtttcg ccgtttctgt tgcggcgaac aaagtctcag gtgttaac
//
LOCUS       DAOMXO010000585         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_44991_length_1616_cov_4.803972, whole genome shotgun sequence.
ACCESSION   DAOMXO010000585 DAOMXO010000000
VERSION     DAOMXO010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44991_length_1616_cov_4.803972"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLH57_10580"
     CDS             complement(<1..143)
                     /locus_tag="PLH57_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRK03098.1"
                     /translation="MHRKVQCWILSRSGRCLLLLTNKRRGEFWQPVTGTVEDGESFEQ
                     AALR"
     gene            complement(173..248)
                     /locus_tag="PLH57_10585"
     tRNA            complement(173..248)
                     /locus_tag="PLH57_10585"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Thr,seq:cgt)
     gene            349..1140
                     /gene="proB"
                     /locus_tag="PLH57_10590"
     CDS             349..1140
                     /gene="proB"
                     /locus_tag="PLH57_10590"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRK03099.1"
                     /translation="MAKPIRNRWVVKAGSQMVCSGGLLLVKAWMHDVVRLRRDFGIEV
                     VWVTSGAIASAAERTRPHRKLAALKKTLPQKQALSAIGQPIVMDLYNTAIQACGALGA
                     QILLTASDLKDQERRSNFVNATEQLLRWGVVPILNENDAVATEEIQFGDNDRLSAQVA
                     SVLGADRLVILTDVDGLYDGDPKKRGSRLVESVGSVTAKIMSYCTGGKSQRGTGGMYS
                     KLLAARLAQQAGIDTHLVRGDRAGVLVTVAQQKKTGTHVHANKRR"
     gene            1121..>1616
                     /locus_tag="PLH57_10595"
     CDS             1121..>1616
                     /locus_tag="PLH57_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRK03100.1"
                     /translation="MQINAAEIRSAQAQLAMTPVAVRNRALVEFSRLLKKNAAEVLRA
                     NGVDVRAAQRAQLTSAFLDRLMLNHKRLEDMAAVLREVASASDPLGRSSKVKTLKNGL
                     KARKITRPLGVIFLIFESRPNVAVESFALALKSGNALILKGGRESAATIKVLEKLMQR
                     ALKNV"
BASE COUNT          428 a          362 c          446 g          380 t
ORIGIN      
        1 cgaagcgcgg cttgctcaaa gctctcaccg tcttccacgg tacctgtaac aggctgccaa
       61 aactcgcctc ttcgtttgtt ggttaacaaa agtaggcatc gtccggatct cgaaagaatc
      121 cagcactgaa ccttgcggtg cattggagaa gtgggttggg tcatattaaa aatggtgccg
      181 actgccagac tcgaactggc gacctacgct ttacgaaagc gttgctctac cacctgagct
      241 aagtcggcgc ttgctagtgg tcaaacaatc gaaggggagc attaacaaac gcatgtttat
      301 tcgacaaccc tatttctggc agatgcattt cggtctaaaa tctagaacgt ggcaaagcca
      361 attcgtaatc gatgggtagt gaaagcgggc agtcaaatgg tgtgttccgg tggccttctg
      421 ttggtaaagg cctggatgca cgatgtggtt cgcttgcgtc gtgattttgg aatcgaagtg
      481 gtctgggtca cttcaggagc gatagcatcg gctgccgaac gaacccgacc ccatcgaaag
      541 ctcgcagcct taaaaaaaac tcttccccag aaacaggcgc tcagtgccat tggccaaccg
      601 attgttatgg atctgtataa taccgcgatt caagcttgtg gtgccttggg ggctcaaatt
      661 ctactgaccg catcagatct aaaagaccaa gaacggcgaa gtaatttcgt aaatgccacc
      721 gagcagttgc tgcgatgggg agttgtgccc atattaaacg aaaacgatgc ggtggcgacg
      781 gaagaaattc agtttggcga taacgaccgt ttgtcggctc aggttgcaag tgttttgggg
      841 gctgaccgac tggttatttt aaccgatgtt gatggtctct acgatggaga ccccaaaaaa
      901 cgtggctccc gactggttga gagtgtggga tccgtcactg ctaaaattat gagctattgt
      961 accggaggaa agagtcagcg cggaactggg ggaatgtact cgaaactcct ggcggctagg
     1021 ttggctcaac aggctggaat cgatacacat ttggtgcgag gcgatcgagc aggtgttttg
     1081 gtgactgtgg cgcaacagaa aaaaacaggg acgcacgtgc atgcaaataa acgccgctga
     1141 aatacggtct gctcaggccc agctcgcgat gactccggtc gcggttcgca accgtgcgtt
     1201 agtagagttt tcacgtttat tgaaaaaaaa tgcggcagag gtgcttagag ctaatggagt
     1261 cgatgttcgg gctgcccaaa gagcgcaact aacttcagca tttttagatc gcttaatgtt
     1321 aaaccacaaa cgccttgaag atatggccgc agtcttgcgc gaagtagcca gtgcgagtga
     1381 tccgttgggg cgcagtagca aagtaaaaac gcttaaaaac ggtttaaagg cgcgaaaaat
     1441 cactcgtccg ttaggagtca tatttcttat ttttgaatcg cggcccaacg tagcggtaga
     1501 gagtttcgca ctcgcactca agtcaggtaa cgcgttgatt ttgaaaggcg ggcgcgaatc
     1561 agcggcgacc attaaagtgc tagaaaaact catgcagagg gccttaaaaa atgtag
//
LOCUS       DAOMXO010000586         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_45213_length_1610_cov_3.743408, whole genome shotgun sequence.
ACCESSION   DAOMXO010000586 DAOMXO010000000
VERSION     DAOMXO010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45213_length_1610_cov_3.743408"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1610
                     /locus_tag="PLH57_10600"
     CDS             <1..>1610
                     /locus_tag="PLH57_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03101.1"
                     /translation="KEQSYRDAEDLFRGQYSDEYIREYTGSDDDWKPNPRTLINDPEF
                     RAHCETEIKAAKEALEENQVTPELQICAQSTPHYRKMFEKKRNSMFGEAVFMSGRFSV
                     VEEKFRDAKASALAVVKRQLADAYRVSFAVSIDSFLNEDIGKQAQGAGKIEPSAFKKK
                     ADLLVRAAVGRMIRHLDSIEINPDPLSVCTVDQMDVMVTRRSGKGPMITVCPGMLQAL
                     STGKFEEEKSAALMNMLTRAIAQAVDSCEADRMTVGHSPKVALGDMLQGQRACVVERS
                     KGMVTPLGGGDVPNAFSDSLAYDRWASARSVNDKSAPHCSVPLTQTEIQERKAMGRRE
                     QTNNLVAQEFANQIFADWFKKQSLTKKNKNEKKKRNSVLRASFASYCTMKSIDGSGMP
                     SAKVFMNEFLAKDQHLRSAMGCKLKEKIIKVKVKGKKSQKQKVSMVQPKCSLADSFRK
                     GMSRYYKGLILNPDWNVTPEESQMIEEATEAIQKGSEEHAKETKSDLEIIDEIEMNPT
                     EEDSFTVNQGGPGATGGQAGATGGQAGAT"
BASE COUNT          492 a          365 c          426 g          327 t
ORIGIN      
        1 aaggaacaat cctaccgaga cgctgaagac ttgtttcgag gccagtattc ggacgaatat
       61 attcgcgaat acacgggctc tgacgacgat tggaaaccca atccgcgtac cttgattaac
      121 gatccggagt ttcgcgccca ctgtgaaaca gaaattaagg ctgcaaaaga ggcgttagag
      181 gaaaaccaag ttactcctga attacaaatt tgtgcgcaaa gcactccgca ttatcggaaa
      241 atgttcgaga aaaagagaaa tagcatgttc ggcgaagcag tatttatgtc gggcaggttc
      301 agcgtggtcg aggaaaaatt tcgcgatgcc aaggcaagtg ctctcgcagt ggtaaaacgc
      361 caattggccg acgcataccg agtgtcgttt gctgtaagca tcgattcatt tttaaatgaa
      421 gatataggta aacaagcgca aggtgcagga aaaattgaac ctagtgcgtt taagaaaaaa
      481 gccgatcttt tagtgcgcgc agcggttggt cgaatgattc gccacctcga ttcgattgaa
      541 atcaatccag accctttatc ggtgtgcacg gtcgatcaaa tggacgttat ggtaaccagg
      601 cgttcgggca agggccccat gatcacggtg tgtcccggta tgctgcaagc actttcgacc
      661 ggtaagtttg aagaagaaaa atcggctgcg ctcatgaaca tgctcacgcg ggccattgcg
      721 caagcggtgg attcttgtga agccgaccgc atgacggtgg gtcattcgcc aaaagtcgcc
      781 ctgggagaca tgctacaagg ccaacgcgcg tgcgtggtcg aacgatcaaa ggggatggtt
      841 acacccttgg gcggcggcga tgtgccgaac gcgttttcgg attcgctcgc ctacgatcgg
      901 tgggctagtg cgcggtcggt gaacgacaaa tcggcgccgc attgttcggt gccgctcacg
      961 caaactgaaa ttcaagaacg caaagccatg ggtcggcgcg agcagaccaa caatttagtg
     1021 gcgcaggaat ttgccaatca aatatttgct gattggttca aaaaacaaag cctcaccaag
     1081 aaaaataaga atgagaaaaa gaaacgaaac tcagtactgc gcgcttcgtt tgcttcgtat
     1141 tgcacgatga agtctatcga cggcagcggt atgccgagcg ccaaagtgtt tatgaatgag
     1201 tttctcgcca aagaccagca cctgcgcagt gctatgggct gtaagctcaa agaaaaaata
     1261 attaaagtaa aagttaaggg caaaaaaagt caaaaacaaa aagtatcgat ggttcagccg
     1321 aaatgttcat tggctgattc gttccgaaag ggcatgagcc gctattacaa agggcttatt
     1381 ttaaacccag actggaacgt aacgcccgaa gagagccaaa tgatcgaaga agcaaccgaa
     1441 gctattcaaa aaggctctga agaacacgca aaagaaacaa aaagcgactt agaaattatc
     1501 gacgaaatcg aaatgaaccc aaccgaagaa gactccttca ccgtaaacca aggcggccca
     1561 ggcgctaccg gcggccaagc gggagctaca ggcggccaag cgggagctac
//
LOCUS       DAOMXO010000587         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_45327_length_1607_cov_4.261598, whole genome shotgun sequence.
ACCESSION   DAOMXO010000587 DAOMXO010000000
VERSION     DAOMXO010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45327_length_1607_cov_4.261598"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PLH57_10605"
     CDS             <1..705
                     /locus_tag="PLH57_10605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK03102.1"
                     /translation="FLFGFFVLPESLAVKHRRKMTARALNPFRSLKHVLSLPAIQVLL
                     AVHVLTQLAGQTHPSIWTLYTQHRYGWNAASVGLSLAVVGILSAFSQGVLTGVFVKRF
                     GERKTVLLGCLGEGLCFALFGLAFNGPMLYAVLLIASIFWSMHPALQALISRDVPDNQ
                     QGELQGSLMSLTSLTSIVNPLIMTSLFAAFSNRTSGLYLPGAPYFLGCALLLAAWLLL
                     ATSGRAPMMPKKALEG"
     gene            1058..>1607
                     /locus_tag="PLH57_10610"
     CDS             1058..>1607
                     /locus_tag="PLH57_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK03103.1"
                     /translation="MQTDLKPAGRGPFAVKLAELQSTDVVLNVGCFDGALERHYLRGK
                     VRDYRGVDMNAQAVQLANRGESVPTENGEPRFKFAPAEKLPFSDATFDKVFCLDTLEH
                     VEDETQALREIARVLKPGGTLVLSVPHDFLNFLDPDELTRGARNLVRKYLRKKPLLNH
                     PKHKHYSESALRALLKDFEIERV"
BASE COUNT          367 a          420 c          375 g          445 t
ORIGIN      
        1 tttcttttcg ggttttttgt actgcccgag tctttggccg tcaaacatcg gcgcaaaatg
       61 acggctcgcg ctttgaaccc gtttcgctct ttaaaacatg tattaagtct tccggccatt
      121 caggtgctgc tcgcggttca cgtactcact caattggcag gccaaaccca cccctcgatt
      181 tggacacttt acacccaaca ccgctacggt tggaacgccg cttctgtcgg tctttcgctc
      241 gctgttgtcg gcattttaag cgcgttttca cagggagttc taaccggcgt atttgtaaaa
      301 cgttttggcg aacggaaaac cgtgttgctc gggtgcctcg gcgaaggttt atgtttcgcg
      361 ctcttcggcc tagcgtttaa tggcccaatg ctctatgcgg ttttgttaat agcttccatt
      421 ttttggtcca tgcatccggc tctgcaggcg cttatcagcc gggacgtacc cgacaatcag
      481 cagggcgaac tacagggttc tctcatgagt ttgacgagtc taacgagcat tgttaatcca
      541 cttatcatga cgtcgctctt tgctgcgttt tcgaaccgta cctccgggtt atatttgcca
      601 ggcgcccctt attttttagg atgcgcgctg ctcctagcgg cctggctttt acttgccacc
      661 tcagggcgcg ctccgatgat gcctaagaag gcgctggaag gataacaatt ggaatcacgc
      721 atagtctctt ttttgcttct taaatgcccc aagtgtcagg atggtaaaat attcaagggc
      781 ttatactcca tgagtgaaaa ttgcccggca tgcggactgc gatttgagcg tgagccaggc
      841 tacttcaccg gagccatgcc catgagttac gcaatcggct tttttctaac gctgccatta
      901 acagtagttc tgattttcac cgaagcacca attctctggt taatacttct tccggccata
      961 cttctgttaa tatgctcgcc cttgatattc aaattttcac gtgcaatttg gttgtatctt
     1021 gatcgcaatt tggatcgaca ccaatagagg agattccgtg caaactgacc tcaaacccgc
     1081 tggccgcggc ccgttcgcag taaaattagc agaacttcag tcgaccgatg ttgtgctaaa
     1141 cgtcggttgt ttcgacggcg cattggaacg ccattacctg cgcggcaaag tgcgagacta
     1201 tcgaggcgtg gacatgaacg cccaggctgt acaattagct aatcgcggtg aatcagtacc
     1261 aaccgaaaac ggggaaccgc gttttaaatt cgcccccgcg gaaaaactac ctttttctga
     1321 tgcgacgttc gacaaggtgt tttgtttaga cactctcgaa cacgtagaag acgaaaccca
     1381 agctctgcgc gaaatcgcac gagtccttaa accgggcgga acgcttgtgc tttcggtgcc
     1441 tcatgacttt ttgaatttcc tcgatccaga cgagctcact cgcggagcgc ggaatttagt
     1501 tcgcaaatat ctccgaaaaa aaccgctttt aaatcacccc aaacacaaac attacagcga
     1561 gtcagcttta agggctttac taaaagactt cgaaattgaa cgggttc
//
LOCUS       DAOMXO010000588         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_45334_length_1607_cov_3.550902, whole genome shotgun sequence.
ACCESSION   DAOMXO010000588 DAOMXO010000000
VERSION     DAOMXO010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45334_length_1607_cov_3.550902"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLH57_10615"
     CDS             complement(<1..309)
                     /locus_tag="PLH57_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03104.1"
                     /translation="MGDEFELFNPSDFESIRLTFDFKNLSTDRSVPNSSDVSLLEVGD
                     KTLTVEIPFDNCAVDNHVLLKIFKTDPASGNQVPIFNPTAKMIKNDKVGDGDRLLIVL
                     D"
     gene            432..>1607
                     /locus_tag="PLH57_10620"
     CDS             432..>1607
                     /locus_tag="PLH57_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRK03105.1"
                     /translation="MNLLNPQFYKNQSDPDAPMREIALKTIDFFETRGKRKLKRADHD
                     REWYADFLEFQKQNQIFSTLLTPTQYGNGHSRWDTSRICKFNELLGFYGLSYWYTWQV
                     SMLGLGPIWMSKNEPLKKQAAEFLKEGGIFAFGLSEKDHGADLYATEMRLFPINEKLH
                     HARGRKYYIGNAQKAALVSTFGKFEGSGDFVFFAVAPTQPNYECLQNVVNSPNYVGEL
                     ALNDYPITEKEILSRGQEAWDTALNTINICKFNLGWASIGSCTHAFYEAINHASKRRL
                     FDHWVTDFPHIQTLFVEAFTRLFAMKLFSTRAIDYLRSASESDRRYLLFNPIVKMKVT
                     SQGEQVINLLWDVIAAKGFEKDTFFETAAREIRALPKLEGTIHVNMALIIKFMKNYFF
                     "
BASE COUNT          460 a          385 c          335 g          427 t
ORIGIN      
        1 atccaacaca atcagtaatc ggtccccgtc gccgacctta tcatttttga tcatcttggc
       61 ggtgggatta aaaataggaa cctggttgcc cgacgcggga tcggtcttaa atatttttaa
      121 aagtacatgg ttatccaccg cacaattatc gaatggaatt tccactgtta gcgttttgtc
      181 gccaacttcg agcaacgata cgtcactcga attgggcaca gaccggtcgg tcgatagatt
      241 cttaaaatca aaggtcagcc gaatcgactc aaaatcagaa ggattaaata actcaaactc
      301 gtcgcccatg taggtatttt agggcaacct tcgtttttta tctacgctag tcgaaaacga
      361 acccattgat actatgcgtt gcaccattcc gctgactatg catctcgcag caattcttat
      421 aaggtttcct aatgaatctg ctcaatccac aattttataa aaatcagtct gatcccgatg
      481 caccgatgcg agaaatcgca cttaaaacca ttgatttttt cgaaactcgg ggcaaacgaa
      541 agctcaagcg agccgatcac gatcgggaat ggtacgccga ttttctcgag tttcagaagc
      601 aaaatcagat tttttcgacg ctattaactc cgacacaata cggaaacggg cattccagat
      661 gggacacctc tcgcatctgc aaatttaatg aactcctcgg attctacgga ctaagttact
      721 ggtacacctg gcaagtttcc atgctgggac tcgggcctat ttggatgagt aaaaatgaac
      781 ccctcaagaa gcaagccgct gagtttctaa aagagggcgg catattcgca tttggtcttt
      841 cggaaaaaga tcacggtgcc gacctttacg ctaccgaaat gcgccttttc ccaattaacg
      901 aaaaactgca ccacgcgcgc ggacgaaaat attatatagg caacgctcaa aaagcggcac
      961 tcgtttcgac atttggaaag tttgaaggtt ctggcgattt tgtatttttc gctgttgctc
     1021 ccacccaacc caattatgaa tgcttacaaa acgtggttaa ctcgcccaat tatgtcggag
     1081 agttggcatt aaatgactat ccgatcaccg aaaaggaaat tctgagtcgc ggccaagaag
     1141 cctgggatac agccttaaat accattaata tctgcaaatt taatctcggc tgggcatcga
     1201 tcggaagctg cacccacgca ttttatgaag ccatcaatca cgcgtcaaaa aggcgtttgt
     1261 ttgaccactg ggtcaccgac tttccccaca ttcaaaccct gttcgtagag gcctttacta
     1321 gactgttcgc gatgaaactt ttctctaccc gtgcaattga ctacctgcgt tcagcctctg
     1381 agtccgatcg gcgttactta cttttcaatc cgattgtcaa aatgaaggta acaagccagg
     1441 gggaacaagt cataaaccta ttatgggacg taatcgcggc caagggcttc gaaaaagaca
     1501 cgttctttga aactgcagct cgagaaatcc gagctcttcc gaagttagag ggcacgattc
     1561 atgtgaacat ggctctcatc attaagttta tgaagaatta ctttttt
//
LOCUS       DAOMXO010000589         1606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_45367_length_1606_cov_4.918762, whole genome shotgun sequence.
ACCESSION   DAOMXO010000589 DAOMXO010000000
VERSION     DAOMXO010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45367_length_1606_cov_4.918762"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLH57_10625"
     CDS             complement(<1..418)
                     /locus_tag="PLH57_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03106.1"
                     /translation="MIRRRSIFHSWLISINIGQLLAIAAVAVIPLLFWFSKTLDPSSL
                     SPAKIQPLLSPIGWSLALASIVGVTSTALAFSISWWLWRFRFNVHFKRVIELCLKIPY
                     LLPGFFFAMGWIALAAPNVGYLNQISAYLGGPALPTI"
     gene            complement(426..1442)
                     /locus_tag="PLH57_10630"
     CDS             complement(426..1442)
                     /locus_tag="PLH57_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRK03107.1"
                     /translation="MKYLLQSWLAICALLFAVGCTKSGEKPGRTLWIYTSLYNHVVQD
                     LDQKLKSKFPDVEIRWYQSGSENVAAKVNSELLGGNTQADLLLTSDPFWYEELKKKGK
                     LLKYTPPTEAKVRAQFLDPDGFYAGVRLPIMVIGYHRDAFSEQEAPKSFNDLLKPTFR
                     DKIAMGSPLESGTAFTSVAVLAKHKGWDYFKKLRENGILVAGGNSAVFGRIETKERPV
                     GIILYENLLAAAAKNPNSRITAVLPSDGVILIPSPVAISATTPHPELAKSVVDFLFSK
                     DAQGAIVSGHMHGLSELSPPLPGAINLSAVLKLAFPWSPEWIQEVYSKRESIKQQFSE
                     IVLD"
BASE COUNT          412 a          367 c          422 g          405 t
ORIGIN      
        1 aaatagtagg cagtgcgggg ccccctaagt aagcagagat ttggttcaaa taccctacgt
       61 tgggcgcggc caatgcgatc cagcccatgg cgaagaaaaa gcccggcaaa agataaggga
      121 tttttaagca aagctcgatt acgcgtttaa aatggacatt aaagcgaaac cgccacagcc
      181 accagctgat cgaaaacgcg agggcggtgg aggtgacacc cacgatacta gcgagcgcga
      241 gactccagcc tatggggcta agcaagggct gtatttttgc tggcgataag gaacttggat
      301 caagagtttt agaaaaccaa aataaaaggg gaatcacggc aactgcagca attgcgagca
      361 attgcccgat atttatggag ataagccagc tgtgaaatat tgatcggcgt ctgatcacgg
      421 cagcgtcagt cgagtacaat ttcggaaaac tgttgtttta tactttcgcg cttcgaatag
      481 acctcttgta tccattccgg ggaccatgga aatgcgagtt tcaatactgc cgataaatta
      541 atggcgcccg ggaggggagg cgaaagttca ctcagtccgt gcatatgtcc gcttacgata
      601 gcgccttgag catccttcga gaagagaaag tccaccactg attttgctaa ttcggggtgg
      661 ggggttgtcg cagaaatggc aacgggactt ggaattaaaa tcacgccgtc gcttggaaga
      721 accgctgtga tgcggctatt tggattcttg gcggcagcag caagtaagtt ttcgtaaaga
      781 attattccca ccggccgctc tttggtttca atgcggccaa acactgcact attaccacct
      841 gctaccagaa ttccgttttc tcgtagtttt ttgaaataat cccaaccctt gtgttttgcc
      901 agcaccgcca ccgacgtaaa ggccgtgcca ctctcgagag ggcttcccat agcaatcttg
      961 tctcggaacg tgggtttcag taggtcatta aaactttttg gtgcttcttg ttcggaaaac
     1021 gcatcacggt gatatccaat caccattata ggaaggcgca cgccggcgta gaatccgtct
     1081 ggatccaaaa actgagcccg aaccttggcc tcagtcggtg gggtgtactt cagtagtttg
     1141 cccttttttt tgagttcttc gtaccagaac gggtcgctcg tgagcaggag atctgcttga
     1201 gtgttaccgc ccagcaattc gctgttaact ttcgcggcga cattctcgct gccggactga
     1261 taccatcgga tttctacgtc tggaaacttc gatttgagtt tttgatctag atcttgaaca
     1321 acgtggttgt agagcgaagt gtagatccag agcgtgcgac ctggtttttc accggatttg
     1381 gtacacccta cggcaaacag taatgcacaa atagccagcc agctttgtag taaatatttc
     1441 atgaactttt actactttga atggcggtcc ggcgtaaagg ccaatgtcat tgcgaaggta
     1501 actgcgggaa acccagatcg cagtagtagg tcttttatat gaattagcgt ggtccctgtg
     1561 gtgcagacat cgtcgaggag tacggtggtc gggggatgaa cactag
//
LOCUS       DAOMXO010000590         1603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_45464_length_1603_cov_7.133075, whole genome shotgun sequence.
ACCESSION   DAOMXO010000590 DAOMXO010000000
VERSION     DAOMXO010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45464_length_1603_cov_7.133075"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLH57_10635"
     CDS             complement(<1..316)
                     /locus_tag="PLH57_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03108.1"
                     /translation="MDPLGKGPKIRLRYNKIECAILGVVGLVVTNSFYRVLSGSQEVA
                     SKPLPPKAVASKLGEKAESYETKCRERGETFVTTASRVRITGPYCQGGSDTSINRSLA
                     STP"
     gene            429..>1603
                     /locus_tag="PLH57_10640"
     CDS             429..>1603
                     /locus_tag="PLH57_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03109.1"
                     /translation="MDILGQSALLVAVAACSLAFKALSKKLRNSLVVQYAVLCFVISA
                     WALTFFLEKIFLSGFCYQLHLGFNVILTPVALRFVRSITRTSTRYTQIYDLAAAIYGA
                     IVLLVLWLGIIEGSFFKILATYAPSFLFFECVRLMLLDLRRIRARTKDAEPIFLTQSL
                     RNTLRSSRRVWIYIGALVLLATAVMDHVPQLGEVIPSIGNGLLCIYLYVFSEAIIHQR
                     LFNRTALVSRVIVLVFTSAVCSGIFSAFLGWIEQEPALFILNAFVASVLFLIVLEPIR
                     SLAHWVATKVSGRGDRVIQDKVRRAEAVLSGAVDSIGLAHSVLSFLEEVFVVRSASVF
                     VLRSDESKYRRIRGLRDDSLMHTEVLASHAVVEFFSRLRRRGETPIVLENYLENEIE"
BASE COUNT          326 a          372 c          414 g          491 t
ORIGIN      
        1 ttggagtaga ggccaggctt cgattgatgg acgtgtccga acccccttgg cagtaagggc
       61 cggtgatacg cactctggaa gctgtggtca caaaggtttc tccgcgttca cggcatttgg
      121 tttcgtagga ctcggccttt tcgccaagct tgctcgctac cgctttaggg ggcaagggtt
      181 tggatgcgac ttcctgagag cccgaaagca cccggtaaaa cgaattcgta accacaagcc
      241 caacgacgcc aagaattgcg cattcgatct tgttatacct caggcgaatt tttgggcctt
      301 ttccaagagg gtccatcaac ctcttatcgg tgccctgtca aaactcctac acggaatttc
      361 ctgcctctcc tgggctagtg cgccaaatag gttgaaatcc aagcgtcggc caggctagct
      421 tccagggcat ggatattctt ggtcaatcag ccctgttggt ggccgtagcg gcttgctcgc
      481 tggcatttaa ggctctttct aaaaagttgc gaaacagcct ggtcgtgcaa tacgcagttt
      541 tgtgttttgt gatcagcgcc tgggcattga cgttttttct ggaaaaaatt ttcctatccg
      601 gattttgtta ccagctgcat ttgggtttca atgtcattct tacgccggtt gctctgcgtt
      661 tcgtgcgaag catcacgcga actagcacca ggtacactca gatctatgat ttggctgccg
      721 cgatctacgg ggcgatcgtg cttttggttt tgtggctggg catcatcgaa ggttcttttt
      781 ttaaaatttt agcaacctac gctccttcat ttttattttt cgaatgtgtg cgtttgatgc
      841 ttcttgatct ccggcgcatt cgggcgcgga ccaaagacgc cgaaccgatt tttttaactc
      901 agtcacttcg taatactctg cgtagttcac ggcgagtttg gatttacatt ggagcacttg
      961 tcttactggc aactgcggtc atggaccacg tgccacagtt gggtgaggtt attccgagca
     1021 tcggtaatgg gcttctttgt atttacctgt atgttttcag tgaagccatt attcaccagc
     1081 ggcttttcaa tcgcacagcg cttgtttcga gagtgattgt actcgttttt acaagtgcag
     1141 tttgttcggg gatattttcg gcctttctcg gatggatcga acaagaacct gcgttgttta
     1201 ttttaaatgc attcgtagcc tcggtacttt ttttgatcgt actagaacct attcgtagtc
     1261 tagcgcattg ggtggccacc aaagtttccg gtcgcggaga ccgcgtaata caagataagg
     1321 ttcggcgtgc ggaggcggta ctttccggtg cggtggactc gatcggactt gcgcattctg
     1381 tgctttcttt tttagaagaa gtgtttgttg tacggtcggc ttcggttttc gtattgcgtt
     1441 cggatgagtc caagtatcgt cggattcggg gtttgcgcga tgattcttta atgcacaccg
     1501 aagtgttggc atcccacgca gttgtcgaat ttttttcgcg ccttcgtcga cgcggcgaaa
     1561 caccgatagt attagaaaac tacctggaaa atgaaatcga aca
//
LOCUS       DAOMXO010000591         1602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_45512_length_1602_cov_4.376858, whole genome shotgun sequence.
ACCESSION   DAOMXO010000591 DAOMXO010000000
VERSION     DAOMXO010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45512_length_1602_cov_4.376858"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PLH57_10645"
     CDS             <1..421
                     /locus_tag="PLH57_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRK03110.1"
                     /translation="WRSTHIQMLSNNTVVLPNSKVASSQLTNFDMPDRETAVLVQVGV
                     SYDCDLDHVERVTVEVAKQVLRKVTGGVPGFDPFIRYHTFGDSSVNFTVILRAQQFTD
                     NFLLKHEFTKALHARYRQENISIPFPQRVIHLKNEKS"
     gene            435..>1602
                     /locus_tag="PLH57_10650"
     CDS             435..>1602
                     /locus_tag="PLH57_10650"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HRK03111.1"
                     /translation="MTTPKPSSEWRKSLQDFLEETAQITKKILLLEIEGDQKQAKATV
                     DAGIHMRGVRLWFLLASCLIASIGLNTNSSAVIIGAMLISPLMGPILGLGFGMAVASR
                     RMIRASAQNLAFAVFISLLVATLYFVVSPFQDPTSEILLRTEPNLLDLLVAIAAAFAG
                     VVAMNSTGFAGAVPGVAIATAIMPPLCASAYGIAHGDWKVALGAFYLFFVNAFAIAVV
                     SFLLFKRLHFSEDAPEPGENGKLRPAVISLVLVLIVAPLGWSLWDAWRDNFVRRSVSR
                     LVQNYKEKLEVVSWQWEDGRAKNLVLYNFKKTPQALRDEIEKALKDIVPQATLELHES
                     SLSPDMEEMVTQMKTSQFSALVSNPKIIDGLLRLASTETSAGDLMTLVLKHEPEL"
BASE COUNT          400 a          382 c          381 g          439 t
ORIGIN      
        1 atggcgaagc actcatattc aaatgctttc caataacacc gtggttttac ccaactcgaa
       61 agttgcgtca tcacagctaa ccaattttga tatgcccgat cgcgaaactg ccgtactcgt
      121 tcaggtcggg gtcagttatg actgcgactt agaccacgtc gaacgggtca ccgtcgaggt
      181 ggcaaaacag gttctccgca aagtaactgg cggtgtaccc ggttttgatc cttttattcg
      241 gtatcatact ttcggcgact ccagcgtcaa cttcacggtc attctgcgcg cgcagcaatt
      301 cactgacaat tttcttttga agcacgaatt caccaaagcg ttgcacgccc ggtatcgcca
      361 ggaaaacata tcaattccgt ttccccagag agtaattcat ctgaaaaacg aaaaaagcta
      421 ggtataatcg aaccatgacc actccaaaac cctcctcaga atggcgcaag agtttacagg
      481 attttctgga agaaaccgct cagatcacca aaaaaattct tctactcgag atcgaaggcg
      541 atcaaaaaca agccaaggcc acggtagatg ccggtattca catgagaggg gtacgcctgt
      601 ggtttttgct ggcatcctgc cttattgctt caatcggact aaacactaac agcagtgccg
      661 tgatcattgg cgcaatgtta atttcaccct taatgggacc aatactgggt ttgggattcg
      721 gcatggccgt ggcgagtcgc cgtatgattc gtgcgtcggc tcaaaacttg gcctttgcag
      781 tttttattag tcttctcgtc gccactcttt atttcgtggt ttctcctttt caagacccga
      841 catcggaaat tcttttgagg actgaaccaa acctactcga tctgttagtg gctattgccg
      901 cagcgtttgc gggcgtggtg gccatgaatt ccactggctt tgctggcgcg gtgccaggcg
      961 tggcaatcgc tactgcaatt atgccgcccc tttgcgcgag cgcttatgga attgctcacg
     1021 gcgattggaa agtagcgcta ggggcctttt atcttttttt cgtaaacgct tttgcaattg
     1081 cagtggtttc gtttctactt tttaaacgct tgcacttttc agaagacgct ccggagcccg
     1141 gcgaaaacgg caaactgcgg cccgccgtca tttcattggt acttgtattg attgtggctc
     1201 cgcttggttg gagtctatgg gatgcttggc gagacaattt tgttcgccgt tcggtgtcca
     1261 gattggttca gaactataaa gaaaaactcg aggtggtgag ttggcagtgg gaagacggtc
     1321 gcgccaaaaa ccttgttttg tataatttta aaaaaacgcc gcaagctttg cgcgacgaaa
     1381 ttgaaaaagc gcttaaagac attgtgcccc aagcaacgct agaacttcac gagagttcgc
     1441 tttcccctga tatggaagaa atggtcactc aaatgaaaac ctcgcagttt tcggctctgg
     1501 tgagtaatcc aaaaattatt gatgggttat tgcgtttggc gagcactgaa acttcggccg
     1561 gcgatttaat gactttagtt ttaaaacacg aaccagaact ta
//
LOCUS       DAOMXO010000592         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_45631_length_1599_cov_5.306995, whole genome shotgun sequence.
ACCESSION   DAOMXO010000592 DAOMXO010000000
VERSION     DAOMXO010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45631_length_1599_cov_5.306995"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1334
                     /locus_tag="PLH57_10655"
     CDS             78..1334
                     /locus_tag="PLH57_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03112.1"
                     /translation="MNFKIVTLIVIAGFATTSLGHSMGKSRSHKKKANAEIARLKKEN
                     EKLVEEQKRLKKKIAKDNEERRRITEQHEEIEKQRLTSLALVNAWNESKGNSNADSAE
                     SFKWNRSRCQAEGDDLPNDTALTGGRFAWVRKCLGERIAYFGEKEKHAKQAQDGMEVV
                     YRSEGVRFRSQLSLLNIFENAKVDGRYFYPLVGKIVFTEEVQDEKIVPQFSIENTYDN
                     GGTEARYVAPTSESAACDWPEGFGLAAMCVGGCYTPEQIVLFSDGYKEIGSVIGGPGL
                     ELVTVHRESTSDSLKFTITPLEAMIASLQEGWNSIVEIQTLSGKMLRVTANHPMLTAK
                     GVMVRADHLGESDLLVNEQGEPEAILAIAAGNYYGKVYNVQPATGDLLENIVVSNGLL
                     TGSNYYQNEGVQNLNRQLLRLNVPSL"
     gene            1348..>1599
                     /locus_tag="PLH57_10660"
     CDS             1348..>1599
                     /locus_tag="PLH57_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03113.1"
                     /translation="MKNYLIMSVAILAAVPALLSGCNHRNRQNATQSPSSETSAEGLR
                     PFFTNDAAGNILYGRTSSALCAPSADLEALSDSEKAFYCL"
BASE COUNT          475 a          349 c          404 g          371 t
ORIGIN      
        1 ttctccggca accagcaccc acgatctgcg gggactgcac gtactgccgc cgacagaatt
       61 taaaaaaagg aaaaaaaatg aactttaaaa tcgttacatt gatcgtaatt gcaggttttg
      121 caacgacttc actaggccac tcgatgggaa aaagtcgttc gcataaaaag aaagccaacg
      181 ccgagattgc tcgactaaaa aaggaaaatg aaaaactagt cgaggagcaa aaacgtctta
      241 agaagaaaat cgccaaagac aacgaagaac ggcgtcgcat cacggaacag cacgaagaaa
      301 ttgaaaaaca acgtttaact tctctagcac tggtgaacgc ctggaacgag tcgaaaggga
      361 attccaatgc ggattccgcc gaatcgttta agtggaatcg ttcccgatgt caggccgaag
      421 gggatgacct tccaaacgac acggcgttga ccggtgggcg ttttgcttgg gtcaggaaat
      481 gtttggggga acggatcgct tattttgggg aaaaagaaaa acatgccaaa caggcgcagg
      541 acggaatgga ggttgtgtat agatcagaag gtgttcgttt tcgcagtcag ctgtcccttc
      601 ttaatatatt tgagaacgcc aaagtcgacg ggcgttattt ctacccactc gtaggaaaaa
      661 ttgtattcac cgaagaagtt caagatgaaa aaatcgttcc gcaatttagt attgaaaata
      721 cctacgacaa cggtggtacc gaagcaaggt acgtagctcc cacatctgag tcggccgcct
      781 gtgattggcc agaagggttt ggtctggccg cgatgtgcgt tggtggttgt tacactcccg
      841 aacaaattgt tctgttttca gacggatata aggaaatcgg aagcgtgata ggcggccccg
      901 gccttgaact cgtgactgtt catcgtgagt cgacgagcga ttcattaaag ttcacgatta
      961 caccactgga ggcgatgatt gcaagtctgc aggaagggtg gaattcgatt gtcgaaattc
     1021 aaacactatc cggaaaaatg ttacgcgtga cagcgaatca ccccatgctt actgccaaag
     1081 gcgttatggt acgcgcagat cacctgggcg agtctgacct tttggtaaat gaacaaggcg
     1141 aacccgaggc tattttggcg atcgcagcgg gcaactatta cggaaaagtt tacaacgtgc
     1201 aacctgcgac cggagacttg ttagaaaaca tcgtggtctc gaacgggttg ttgacgggta
     1261 gtaactatta tcaaaacgaa ggggttcaaa atttgaatcg tcagctcctt cgcctcaatg
     1321 ttccttcgct gtaattaaga ggaaaaaatg aaaaattatt taatcatgtc cgtagcgatt
     1381 cttgcggccg ttcccgctct tttgagtgga tgcaaccatc gtaatagaca aaatgcaaca
     1441 caatcaccat cgagcgaaac aagtgccgag ggtcttagac cattttttac gaacgacgcg
     1501 gccggaaata tcttgtatgg ccgaactagc tcagcgctat gtgccccgag cgccgattta
     1561 gaggcacttt ccgattccga aaaagcgttc tactgctta
//
LOCUS       DAOMXO010000593         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_45634_length_1599_cov_5.237694, whole genome shotgun sequence.
ACCESSION   DAOMXO010000593 DAOMXO010000000
VERSION     DAOMXO010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45634_length_1599_cov_5.237694"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="PLH57_10665"
     CDS             <1..1240
                     /locus_tag="PLH57_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRK03114.1"
                     /translation="EVTPDGLRALARTPGIAKIYFNRDVLEEPTINGPNPDDGPDPEL
                     VGMPYDFPEMGLDLVMKEMPQITGKGVVVGAIDTGVDGHHPALQGKILTFFDARTGQI
                     TEPYDRDDHGTHTAGTIVGGDRSRTLIGVAPEAKLVASAALSSYDHMLRAMEFMLDPD
                     SDPNTADMPRAINNSWNCEGAPDVELFYKAISAWEAAGILPVFSAGNSGRRGMGTITK
                     PHEHPFVLAVGATGPDGRIADFSSRGPGRFRGQNTQKPDLTAPGVDIESTMPGGTFGK
                     MSGTSMAAPHITGAAALLLQVDPALTPIQLKELMMATATPMSEEDGRDEGNWNPHYGV
                     GKTNIYRALQAAGEIRRRRLSGSVIDLMATILTSPREIVVSKFLAKPPKLDIAELAYS
                     TDTQGDGGPDWATSADFEKP"
     gene            complement(1215..>1599)
                     /locus_tag="PLH57_10670"
     CDS             complement(1215..>1599)
                     /locus_tag="PLH57_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03115.1"
                     /translation="WFAARERYLQQYLPVYEWQAWVNQNLGSDARLVSLNEKQNYYLK
                     GFLENTVEMKSWEELINKNKDSARTLAKEVAALGFTHLHYYPSQSRFTPWYELPKDLE
                     SLARDCVLCTKDSVIVDLTVFQSQR"
BASE COUNT          388 a          444 c          413 g          354 t
ORIGIN      
        1 tgaagttact cccgatggtc ttcgcgcgct tgcccgcact ccgggaattg cgaaaatcta
       61 ttttaaccgt gatgttctcg aagaacccac aatcaacgga ccgaatcccg acgacggtcc
      121 agatccggaa ctcgtaggaa tgccttacga tttcccagaa atgggactcg atctcgtcat
      181 gaaggaaatg cctcagatca ccggtaaagg cgtggtcgta ggagcgatcg ataccggagt
      241 agatgggcac caccccgccc tgcagggaaa aattttaacc ttcttcgacg ctcgcacggg
      301 tcagatcacc gaaccgtacg atcgtgacga tcacggaact cacacggcgg gaaccattgt
      361 gggcggagat cgttctcgca ccctcatcgg cgtagcgccc gaagcaaaac tcgtcgcctc
      421 ggcggctctg tctagttacg atcacatgct tcgcgcaatg gagttcatgt tagatcccga
      481 ttcagatccg aacaccgctg atatgccacg tgcaatcaac aactcgtgga actgtgaagg
      541 tgctcccgac gtagagctct tttataaagc aatctcggcg tgggaagcgg caggaatact
      601 tccagtgttt tcggctggta actccgggcg ccgcggtatg ggaacgatca ccaaacctca
      661 cgaacacccg ttcgttcttg cagtcggcgc caccggtccc gacggacgaa tcgctgattt
      721 ttcgagccgg ggtcctggaa ggttccgtgg tcaaaatacc caaaaaccag atctcacggc
      781 cccaggcgta gatattgaat cgaccatgcc tggcggtacg ttcggaaaga tgagtggtac
      841 ctcaatggcg gctcctcaca ttacaggcgc agcggccttg ctcttacaag tggaccctgc
      901 gttgactccg attcagctca aggaactcat gatggccact gcaaccccca tgagcgaaga
      961 agacggacgc gacgagggca actggaaccc gcactatgga gtcggcaaaa cgaatattta
     1021 tcgggcgcta caggcggcag gtgaaattcg cagacgccgt cttagtggtt cggtcatcga
     1081 tctaatggcg acaattttaa catcgccgcg tgaaatcgtc gtcagcaagt ttctggcgaa
     1141 acctccgaag ctcgatatcg ctgagctcgc gtattcgacc gacactcaag gcgacggcgg
     1201 ccctgactgg gctactagcg ctgactttga aaaaccgtaa gatcaacgat caccgagtcc
     1261 ttggtgcaca aaacgcagtc ccgcgccaag gactcgagat ctttcggaag ctcgtaccac
     1321 ggcgtaaagc gcgactgact agggtagtag tgtaggtgag tgaatccaag tgccgcgact
     1381 tcctttgcaa gtgttctcgc ggaatcttta ttcttattaa ttaactcttc ccagcttttc
     1441 atttcgacgg tgttttcaag aaatcctttt agatagtaat tttgtttctc attgagggac
     1501 accagcctcg catcgctccc aagattttgg ttaacccatg cctgccattc gtacaccgga
     1561 agatactgtt gcaagtaacg ctcacgcgcg gcgaaccaa
//
LOCUS       DAOMXO010000594         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_46093_length_1588_cov_5.176778, whole genome shotgun sequence.
ACCESSION   DAOMXO010000594 DAOMXO010000000
VERSION     DAOMXO010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46093_length_1588_cov_5.176778"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /gene="rho"
                     /locus_tag="PLH57_10675"
     CDS             complement(<1..784)
                     /gene="rho"
                     /locus_tag="PLH57_10675"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRK03116.1"
                     /translation="MYLKELKEKKIGELADLAKGLGIENAAGLRKQDLIFSILQKSAE
                     QNQAIYGEGVLECLPDGFGFLRSPDYNYLPGPDDIYVSPSQIRRFNLRTGDTISGQIR
                     PPKDSERYFALLKVERLNMEDPAESKERILFDNLTPLYPDERIVMEYDPTNYSTRIID
                     MLVPIGKGQRCLIVAPPRAGKTVLLQDLAHAITTNHPEISLIVLLIDERPEEVTDMQR
                     TVKGEVVSSTFDEQASRHVQVAEMVLEKAKRLAEHKRDVVVLL"
     gene            992..>1588
                     /locus_tag="PLH57_10680"
     CDS             992..>1588
                     /locus_tag="PLH57_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRK03117.1"
                     /translation="MRRTFTQSAGGVVVLIMLGLTVALSAVGALHIWRFLGHGQNVST
                     PKVVFYEVKKGATPRQIARDLESIGVVGNARIFRLFGRLTGQNSKIKVGDYRFTNALS
                     PNEVFRILTSGVSYGLPLIIPEGYSIAQIAGVFEEVRKGSRAQVLAMASDRAFISSVW
                     ASAWKLERVGKPPKIPGTLEGYLFPDTYFITREADVKSV"
BASE COUNT          377 a          347 c          407 g          457 t
ORIGIN      
        1 cgagaagtac gacaacgtct cgtttgtgtt ccgctaaacg cttggccttt tctagaacca
       61 tttcagcaac ctgaacgtgg cgactggctt gctcgtcgaa ggtcgagctt acaacttcac
      121 ctttaacagt gcgttgcatg tcggtcactt cctctgggcg ttcatcaatg agaagcacga
      181 tcagagaaat ctccggatga ttcgtcgtta tagcgtgggc caaatcctgc aataaaactg
      241 ttttacctgc gcgcggaggt gcaactatca aacagcgctg accttttcct attggaacga
      301 gcatgtcgat gatgcgcgta gagtagttcg ttgggtcgta ctccatgacg attcgttcgt
      361 cggggtaaag cggggttaag ttatcaaaga gaatacgttc tttgctttcc gctgggtctt
      421 ccatgttaag acgctcaact tttaggagcg cgaaataacg ttctgaatct ttaggcgggc
      481 gaatttggcc cgaaatggtg tcgccggttc taaggttgaa tcggcgaatc tgtgaaggag
      541 aaacgtagat atcgtcggga cctggcagat aattgtaatc aggggaccgt aaaaagccga
      601 atccatcggg gagacattct agaacaccct cgccataaat tgcctggttt tgttccgcgc
      661 ttttttgaag aatcgaaaaa attaggtcct gttttcgaag tcccgcagcg ttttcaatgc
      721 ctaggccttt ggctagatct gctagttctc cgatcttttt ttccttcagt tcttttaaat
      781 acattttgct aaatttctcc tgatacgatg ggaatttttg atttgtttta gaggtaaata
      841 attatctgtc gtgttctaga tcacgttttc ccggtaggtt ttatctgact ggaacgagta
      901 cctatctaac cataatgggg tgcgttgtca actttgttgt caagtctgtc cgggggcagt
      961 tacaataaga agcatacagg gaaggtcgtt gatgcgtcgt acattcacgc aaagtgctgg
     1021 tggagtggtc gtacttataa tgctcgggct gacggtagca ttatcggccg tgggcgcgtt
     1081 acatatttgg cgctttcttg gccacggaca aaatgtgtcc acacccaaag tagtgtttta
     1141 cgaagtaaag aaaggggcca ccccacgtca gatagcgcgc gatctcgaga gcattggagt
     1201 ggtcggtaac gcacgaatct ttcgcctctt cggtcgactt accgggcaga attccaaaat
     1261 aaaggttggc gactaccgct ttaccaatgc tttatctccc aacgaggtgt ttcgtatatt
     1321 aacctccggt gtttcatatg gtttaccgct cattatcccc gagggttatt cgatagctca
     1381 gattgcagga gtgtttgagg aggttcgaaa agggtctcgc gcgcaggttc tcgcgatggc
     1441 ctcagaccgc gcatttataa gttcggtgtg ggcctcggct tggaagctag aaagggtagg
     1501 aaaaccccca aaaattccgg gaactttgga aggatatttg tttccagata cctattttat
     1561 tacccgtgaa gccgacgtta agtcggta
//
LOCUS       DAOMXO010000595         1587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_46128_length_1587_cov_4.783943, whole genome shotgun sequence.
ACCESSION   DAOMXO010000595 DAOMXO010000000
VERSION     DAOMXO010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46128_length_1587_cov_4.783943"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..882)
                     /gene="larB"
                     /locus_tag="PLH57_10685"
     CDS             complement(82..882)
                     /gene="larB"
                     /locus_tag="PLH57_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRK03118.1"
                     /translation="MKTKQDLEALLTALVEGNATVQSVIEKLTSSAFRDGSLGFANLD
                     HHRALRNGFGEVVFGASKTVSEIVTIVRELSLSERTVLVTRLNSEKITACASVLAKEN
                     IPVRVRERASTLTAFPKEPILHSEYPNRAFVAVVTAGTSDLGVAEEAIETLIAHEIAF
                     EAIHDVGVAGLHRVLAQVERIRRASAVIVCAGMEGALPSVLGGLVSAPVFAVPTSVGY
                     GASFSGIAALLGMLNSCASGVTVCNIDNGFGAAMAAARVLNTIREVRA"
     gene            complement(895..>1587)
                     /locus_tag="PLH57_10690"
     CDS             complement(895..>1587)
                     /locus_tag="PLH57_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HRK03119.1"
                     /translation="RSRDGGRVVALTTISPSTSAADQNDARAFVQRVGTPHIWTASQE
                     FENGAYLKNDENRCYHCKAELFRITAEKIAELECRFVAYGYTASDRGDDRPGHRAALE
                     NNVRAPLDEADLEKDDIRILMRAHQLPFYDKPASPCLSSRLMRGVTVTNERLRAVEAV
                     EQRLQAMGLALVRARLHSDHSGEWVRVEVDPKNIEEVTRELFALRELAQSLGLSRIEV
                     DPRGYRMGGANV"
BASE COUNT          376 a          475 c          351 g          385 t
ORIGIN      
        1 ctaggccagc attcacgaaa cttgcagcaa acatatcgcc cgcaactccc gctacgcttt
       61 cgaaatacag ggttctggta ctcacgcgcg aacctctctg atcgtattta atactcgagc
      121 ggccgccatg gcagcgccga agccgttatc aatattgcac acggtcacac cggaagcgca
      181 ggaattcagc atacctaata gcgcggcaat tccagaaaaa ctcgctccgt aacccaccga
      241 agtcggtaca gcgaataccg gagcactcac taaaccgcct aacacactcg gaagcgcacc
      301 ctccataccc gcgcaaacaa tcacggccga cgctctgcga atgcgctcaa cttgtgcaag
      361 cacgcggtgc aaacccgcta ctcctacgtc gtgaatcgct tcaaaagcaa tttcatgggc
      421 aattaatgtt tcgatggctt cttccgccac acctagatcg ctggttccgg ccgtcaccac
      481 tgctacaaaa gcacgattcg gatattcgga gtgcaatatc ggttctttcg gaaacgcagt
      541 taaagtactg gctcgctcac gcactcgtac gggtatgttt tccttggcca aaacactcgc
      601 gcacgcagtg attttttcgg aatttaatct ggttaccaac accgtgcgtt cgctgagact
      661 cagttctctc actattgtca cgatttccga aactgtcttc gaagctccga aaaccacttc
      721 tccaaatccg ttacgaaggg ctcggtgatg atccaaattg gcaaacccca aactgccgtc
      781 gcgaaaagca ctgctggtca atttttcgat tacgctttga actgttgcgt tcccttcgac
      841 caatgcggtg agtagcgctt cgagatcttg ttttgttttc ataaacactc gttcctaaac
      901 attcgcaccg cccattcgat acccacgtgg gtcaacttca atgcgactca aacccaaact
      961 ttgagccagc tctcgcaggg cgaacagttc tcgcgtgacc tcttcaatgt ttttcggatc
     1021 aacttccaca cgcacccact cgcccgagtg atcgctgtgc aaacgcgcgc gcaccaatgc
     1081 cagtcccatt gcttgcaagc gttgctctac ggcttctact gcgcgaagtc gttcattggt
     1141 cacggtcacc cccctcatta accgtgaact caaacacgga ctcgcgggtt tgtcataaaa
     1201 cggtagttgg tgggcccgca tcaaaatgcg tatatcatct ttctcgagat cggcttcatc
     1261 gagtggcgcc cgcacattgt tttctagggc cgcacggtgg cccggacgat catcgccgcg
     1321 atccgaggcg gtataaccgt aggctacaaa tcgacattct aattctgcaa ttttttcagc
     1381 tgtaattcga aagagctctg ccttgcaatg ataacaacgg ttttcatcgt ttttgagata
     1441 cgccccattt tcgaactctt ggctcgcggt ccaaatatgg ggggtcccca cccgttgaac
     1501 aaaagctcgg gcatcgtttt ggtccgctgc cgaagtgctc gggctaattg tcgtaagtgc
     1561 caccacacgg ccaccatcac gtgatcg
//
LOCUS       DAOMXO010000596         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_46249_length_1584_cov_4.067364, whole genome shotgun sequence.
ACCESSION   DAOMXO010000596 DAOMXO010000000
VERSION     DAOMXO010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46249_length_1584_cov_4.067364"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLH57_10695"
     CDS             <1..381
                     /locus_tag="PLH57_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HRK03120.1"
                     /translation="KGKNLAVWGLAFKPNTDDMREAPSLIILPKLLEAGASIRAFDPV
                     ATHEAQKHLPKSAALTFHDNMYEALNGADALIIVTEWNEFRNPDFARVKATMKNPLIF
                     DGRNVYQTESVRANGFTYYSIGRQ"
     gene            385..1200
                     /locus_tag="PLH57_10700"
     CDS             385..1200
                     /locus_tag="PLH57_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRK03121.1"
                     /translation="MLVAAIQLTSKSDKKKNLAVASALMERALRQGAELVALPEYFSF
                     IGKRADIPKEAEQPKKGLAFDTAANFAAKNGVFVLAGGIPTLTANKKLVNRSYLIGQK
                     GEVLGFYDKIHLFDSELTESRAFRESDTFARGTKPGLVETPFGKAGLTICYDLRFPEL
                     YRVLALAGARVIFVPSAFTMYTGKDHWLSLLKARAIENQVYIVAPAQIGRHTQKKVSW
                     GKTSIIDPWGSVLATAPDCETVVLADIDFKFLAKVRKELPVLNHYRKDVFSLL"
     gene            1234..>1584
                     /locus_tag="PLH57_10705"
     CDS             1234..>1584
                     /locus_tag="PLH57_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03122.1"
                     /translation="MRFALILMATAVISMPSAAKGNTKSVLRVYDQDRFVLNTETVNV
                     CIFQIGDNDFGINQLKTEVIEDYKKVIQKDKSIISVNTADGSEAEIPLVIQRTIKFDT
                     RFLSTSTERWGAIGT"
BASE COUNT          440 a          384 c          371 g          389 t
ORIGIN      
        1 aaaggaaaaa atttggcggt ctgggggctg gcgttcaaac caaataccga cgacatgcga
       61 gaagcaccaa gtctgattat tttgccaaaa ttacttgaag caggcgcctc tattcgggcg
      121 tttgatccag tagccactca tgaagcacaa aaacatctac caaagtctgc tgcgcttacg
      181 tttcacgaca acatgtacga agctttgaac ggtgccgatg cgttaatcat cgtaactgag
      241 tggaacgagt ttcgaaatcc tgacttcgct cgcgtaaaag ctacgatgaa aaaccccctc
      301 atatttgacg gccgcaacgt ttatcaaacc gagagtgtgc gcgccaacgg gttcacttat
      361 tattctatcg gacggcagta gagcgtgtta gtcgcagcaa ttcaacttac gtcaaagagc
      421 gataaaaaaa agaacctcgc tgttgcatcg gccttaatgg agcgagcttt gcgccaagga
      481 gctgagttgg tagcgttgcc agagtatttt tcgtttatag gcaaacgagc cgacatccct
      541 aaagaagctg aacaaccaaa aaagggctta gcgttcgata ctgccgccaa tttcgcagct
      601 aaaaatggtg tctttgtatt ggcgggtggg atccccactc ttaccgcaaa taaaaagctt
      661 gtgaatcgtt cctatttaat tggccaaaag ggagaggttt tgggttttta cgataaaatc
      721 cacctcttcg atagcgaact caccgagtcg cgagcgttcc gtgaatccga cactttcgca
      781 cgcggcacca agcctggact tgttgaaacg ccgtttggca aagcaggtct tacgatttgt
      841 tatgatctgc gttttccgga gctctaccgg gtactcgcgc tcgcgggcgc tcgtgtgatt
      901 ttcgtaccgt cagccttcac catgtacact ggcaaggacc actggctttc tttgttaaag
      961 gcgcgagcga tcgaaaatca ggtctacatt gtggcgccag cccagattgg tcgccacacc
     1021 caaaagaaag tttcctgggg aaaaacgagc attatcgacc cctggggcag cgtcctcgcg
     1081 accgctccag actgcgaaac cgtcgtactg gccgacattg attttaaatt cctcgctaaa
     1141 gtacggaaag aattgccggt cttgaaccat taccgcaaag acgtgttttc gctactctaa
     1201 gcaatacccc cgcgtatgcc gaaatgttgg tatatgagat tcgctctaat tttaatggca
     1261 accgctgtga tcagtatgcc ctcggccgct aagggcaaca ccaaatctgt gctccgggtt
     1321 tacgatcaag accgatttgt gttgaacact gaaaccgtga acgtttgtat ttttcaaatc
     1381 ggcgacaatg actttggcat caaccagcta aaaactgaag taatcgaaga ctacaaaaag
     1441 gtaatacaaa aagacaaaag cataatttca gtcaataccg cagacggttc cgaggcagaa
     1501 attccattag ttattcaacg aacgataaaa ttcgacactc gttttctttc aacttcgacc
     1561 gagcgctggg gagcaattgg aact
//
LOCUS       DAOMXO010000597         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_46260_length_1584_cov_3.125572, whole genome shotgun sequence.
ACCESSION   DAOMXO010000597 DAOMXO010000000
VERSION     DAOMXO010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46260_length_1584_cov_3.125572"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /gene="scpB"
                     /locus_tag="PLH57_10710"
     CDS             <1..769
                     /gene="scpB"
                     /locus_tag="PLH57_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRK03123.1"
                     /translation="RTKPHMASVLRKLVKTTPLKLSPTNLEVLAVVAYKQPVTKDELD
                     QIRGVDSGYVIRNLMEKRLVRIIGRSELPGRPMLYGTTHEFLELFSLKDLKALPPLHE
                     VEQLVAQSEVGDEQAEQAALQDFGVMVEKSDKILFDDNGIDIEVESLRAEIAAIPIST
                     AFIEEQKAQEKRSAKLIELAAQGLTLDENDNVVAIEALKPNEHLPSPELQRAAELAMN
                     ERHLEAISETVDQAVSLQPNSDSVVEDVREREPESTI"
     gene            complement(777..>1584)
                     /locus_tag="PLH57_10715"
     CDS             complement(777..>1584)
                     /locus_tag="PLH57_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03124.1"
                     /translation="RDVLRLRAGQRWFDGGAGDAFVGQVYRGLTPFRERLSSGIAALE
                     IELHGSKIRRPEGKPTHPGNISLKKKLDQISITDRADYVGLTYGASAWVPRSSQNGRY
                     RLFVGKYFSEVSNEDIGATDLITDLFGLIPYSVDPDTVLARYLTLSRREGVIYIADGG
                     PRSQFLSESTVKKRDGRIVSFLDWMREIPGIRVQHRPQSEDTVLSTLLMREIRTVSSA
                     RITKIQAFTAKNNIRFPKLKMVGKLSDADRFETKEFIETMENHVVIPLEP"
BASE COUNT          419 a          412 c          374 g          379 t
ORIGIN      
        1 gcgcaccaag ccccacatgg cttcggtcct gcgtaaacta gttaaaacca cacccctcaa
       61 gctttcgccc accaatctcg aagtcttggc ggtcgtggcc tacaaacaac cagtaaccaa
      121 agatgaactc gatcagattc gcggcgtcga tagcggatat gtgattcgaa atctcatgga
      181 aaaacgctta gtccgtatca ttggccgttc agagctacca ggtcgaccta tgctttacgg
      241 taccactcac gaatttttag agctcttttc attgaaagat ttaaaagccc ttccaccttt
      301 gcatgaagtt gaacaactcg tggcgcaaag cgaagtgggc gacgagcaag cggaacaggc
      361 ggcccttcag gactttggcg tgatggtcga aaaatctgac aaaattctgt ttgacgataa
      421 cggaatcgac attgaggtcg agagtctccg cgctgaaatt gcggcgatcc ctatttcgac
      481 tgcttttatc gaagaacaaa aggcgcagga aaaacgttcg gcaaaactca ttgaacttgc
      541 ggcgcagggt cttactcttg atgaaaacga caacgtcgtc gcaattgaag cgcttaaacc
      601 gaatgaacat ctaccatcgc ccgaattgca acgagcggct gaactcgcta tgaacgagcg
      661 ccacctcgaa gcgatttcgg aaacggtgga tcaagctgtg tcactgcagc cgaacagcga
      721 ctccgtcgtt gaagacgttc gcgagcgcga accagaatcg acaatttaat ttaaatctag
      781 ggttcgagcg gaatcaccac gtggttttcc atggtttcga taaattcttt agtttcaaat
      841 cgatcggcgt cagagagttt tccgaccatt tttaactttg gaaaacgtat attgtttttg
      901 gcggtgaagg cctgaatctt agtgattcgc gcagaagaca ccgttcttat ttctcgcatc
      961 aaaagagttg aaagcaccgt gtcctcggat tgcgggcggt gttgtacgcg aattccgggt
     1021 atttcacgca tccagtctag aaatgaaacg attcgcccgt cacgtttttt cactgtcgat
     1081 tcggaaagga actgtgaacg aggaccgccg tcggcaatgt aaattacgcc ctcgcggcgg
     1141 ctcagcgtaa gataacgcgc caatactgtg tcggggtcca cggaatacgg tattagtccg
     1201 aatagatctg taatcagatc ggtagcacca atatcttcgt ttgatacttc cgaaaaatat
     1261 tttcccacga acaatcgata gcgaccgttc tgggaactgc gcggcaccca tgcgctcgca
     1321 ccataggtga gacccacgta gtccgcgcga tcagtaatcg aaatctgatc cagtttcttt
     1381 tttaatgaaa tatttccggg gtgagtcggc ttcccctccg gacgccgaat tttcgatcca
     1441 tgcaattcta tttcgagagc ggcaattccc gaagataggc gttcccgaaa cggcgtcaac
     1501 ccgcgatata cctggcctac aaatgcatcc cccgcccccc catcgaacca acgttgccct
     1561 gcacgtaaac gcaaaacatc ccgc
//
LOCUS       DAOMXO010000598         1581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_46396_length_1581_cov_2.722805, whole genome shotgun sequence.
ACCESSION   DAOMXO010000598 DAOMXO010000000
VERSION     DAOMXO010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46396_length_1581_cov_2.722805"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLH57_10720"
     CDS             complement(<1..431)
                     /locus_tag="PLH57_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03125.1"
                     /translation="MKAHAKRSPNKHIIDTRSRFLYTSARGYLVCFWGEGFLVYFFSL
                     FFSFIATLSAFADTDSLRARIRDIACAEILKVEDIQFREFNDAPGFFMLDSAVPKGLT
                     SVVELRKLTMSKNFSGFTLHELDSAAAYARIVFRSRGERVV"
     gene            complement(400..738)
                     /locus_tag="PLH57_10725"
     CDS             complement(400..738)
                     /locus_tag="PLH57_10725"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRK03126.1"
                     /translation="MANEIDKTNLITTDTKLEGTVTFSGFTRFDGSISGNIVGQPGSE
                     IVLGETGVVEGRVNGDVVIIDGFIRGEVEASSRIVLTETGRVIGKLRAPSIAIGFGAF
                     FEGTCETITQ"
     gene            complement(739..1464)
                     /locus_tag="PLH57_10730"
     CDS             complement(739..1464)
                     /locus_tag="PLH57_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03127.1"
                     /translation="MSDLRLVIYKDQHSPRSISLSTRTLYRTLVLGSFASLFFIGATI
                     LLIKLYLRGPTKISVPSHDDQSDSIIGPTNSPEELIRALRDEIELSKSRLQNAQALQN
                     APKELDSKNPALGLFSPIVTDKTQSGPFVKIANFKYSPGDGKSPLTLTFELHNAGSAE
                     IVQKGYIVVLARNDSSLLGYPNIFRNSGPYLIDFEKGETFQVARFRLVNAQFSAERAK
                     NFQILIFSRSGELLINTMHEVSN"
BASE COUNT          434 a          357 c          368 g          422 t
ORIGIN      
        1 aatacgacac gctcacctcg acttctaaaa actatccgag cataggccgc ggcagagtcc
       61 agctcatgca gcgtaaaccc cgaaaaattt ttactcatgg taagcttacg caactctacc
      121 acggaggtta aaccctttgg cactgctgaa tcgagcatga agaaacccgg agcgtcgttg
      181 aactctcgaa attgaatatc ttcaacttta agaatttccg cacaagcaat gtctctgatt
      241 cgcgcgcgca aagagtctgt gtcggcaaag gccgaaagcg tcgcaatgaa agaaaaaaat
      301 aaagaaaaga aataaaccaa gaacccctca ccccaaaaac aaactaaata cccgcgggct
      361 gaggtgtaga ggaatcgact gcgcgtgtca atgatgtgtt tattgggtga tcgtttcgca
      421 tgtgccttca aaaaaggcac cgaagccgat ggcaatgctt ggggctcgaa gtttaccgat
      481 cactcgacca gtttcagtaa gcacgattcg ggagctcgct tctacttctc cacgaataaa
      541 accatcgatt atcacaacgt cgccgttaac tcggccttcc accacgccgg tttcgcctaa
      601 aacgatttcg ctgccgggtt gcccaactat gttgcccgaa atcgatccat caaacctggt
      661 aaaacccgaa aacgtcacgg taccttcgag tttggtgtcc gtagtgatca ggttcgtttt
      721 gtcgatctca ttggccattt agttggaaac ctcgtgcatt gtattgatga gcaactcacc
      781 tgatcgcgaa aaaattaaga tttgaaaatt tttggctcgt tctgcagaaa actgcgcgtt
      841 gaccaacctg aatcgtgcca cttggaaagt ttcacctttt tcgaaatcga tcaaataggg
      901 tcccgagttt ctaaaaatat ttgggtaacc taatagtgag ctgtcgtttc gtgcaagcac
      961 gacaatataa cctttttgta cgatctccgc tgaaccggca ttgtgtaatt caaatgttag
     1021 cgtgagaggc gattttccgt cgccggggga atatttgaag ttagcaattt taacgaacgg
     1081 tccactttgt gtcttgtcgg tcacaatcgg cgagaagagc cccagggcag gatttttgct
     1141 gtccaattct tttggtgcat tttggagcgc ctgggcattt tgtaggcgag atttcgataa
     1201 ttcgatttcg tcacgcaaag cccgaataag ctcttcagga gagttcgttg ggccgattat
     1261 ggaatcactt tggtcgtcgt gagaaggaac cgatattttt gttggtccac gtaggtaaag
     1321 tttgatcagc aaaatagttg cgccgataaa aaacaacgat gcgaaactgc ctagcaccaa
     1381 agtacggtag agagttcgcg tacttagcga gatgcttcga ggggagtgtt ggtctttgta
     1441 aatgaccagg cgcagatcgg acatattgaa aattcaacaa tgtcgcgggt ttgatgtcaa
     1501 cttttctgtg tttttatccg aactctatca tgatgaggtt tctattaatt gacacagatt
     1561 cgaccttcag ttcgatgcta g
//
LOCUS       DAOMXO010000599         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_46455_length_1579_cov_4.767060, whole genome shotgun sequence.
ACCESSION   DAOMXO010000599 DAOMXO010000000
VERSION     DAOMXO010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46455_length_1579_cov_4.767060"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLH57_10735"
     CDS             complement(<1..464)
                     /locus_tag="PLH57_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03128.1"
                     /translation="MFKNFPQLLLILIGFACAVYTQTTALSDVPGLHFDEAWQGLYAY
                     RIAFEPGFFPIQAMNSYTFSIVHYPIALLFKLFWPTLEVMRIFYVACNLLALSLFVFA
                     FLKIKKPLAAFIFTLIWGLLPFFVHTHRFFIEMTGFFSLCMAAAVYALTTFE"
     gene            complement(457..>1579)
                     /locus_tag="PLH57_10740"
     CDS             complement(457..>1579)
                     /locus_tag="PLH57_10740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRK03129.1"
                     /translation="FKKAGDAGVVLLMSDSTNVERDGHSLSEVNIYRKFKELFAEPTG
                     LTIVSVFASNIARIGQVMELADHFGHKVALLGRSMEQNVRLAQEAGYLEHARSVLIGV
                     DEIEQFPRNKVIVLSTGSQGEFRSALARIASDEHRSVSVGPGDTVIMSSKFIPGNEKA
                     IGRMINSLFKMGADVLYESVEDIHVSGHANADELRKMLRATRPRHFIPVHGEYRHLVK
                     HARLAEQNGVAAENIVIAVNGDVLELTKNSLKRVDHFEENRIFIEGEGGNDISKVVIK
                     DRRKVAEAGIVFSVLIRNSVSGKIVGGPDLISRGLFDGDDGLEILEKGKKTVLSILRD
                     VERSEKQIDLQEEIRIGLRRFFAAHLGKKPVVLPILIDV"
BASE COUNT          457 a          369 c          338 g          415 t
ORIGIN      
        1 ttttcgaaag ttgtcagtgc ataaacggcg gcagccatgc acaacgaaaa aaaaccggtc
       61 atttctataa aaaaccgatg agtgtggaca aaaaatggca aaagacccca aataagtgtg
      121 aatataaaag ccgcgagtgg tttttttatt ttcaaaaaag cgaagacgaa aagtgacagc
      181 gctaataaat tgcaggcaac gtaaaaaatg cgcattactt ccaatgttgg ccaaaataat
      241 ttgaaaagta gcgctattgg atagtgaaca atggaaaaag tgtagctatt catcgcctga
      301 atcggaaaaa atccgggctc gaaagcaatt cggtaagcgt agaggccctg ccaagcttca
      361 tcgaaatgaa gacccggcac atctgaaagt gcggtggttt gggtgtacac cgcgcatgca
      421 aaaccaatta gaataagcaa caactgtgga aaattcttaa acatcgatca aaatgggaag
      481 cacaactggt ttttttccta aatgagctgc aaaaaacctt ctcaatccta ttcgaatttc
      541 ttcttggaga tcgatctgtt tttcagatcg ctcgacatct cgcagaatgc tcagaacagt
      601 tttttttcct ttttcgagaa tttcgagacc gtcatcgcca tcgaacaagc ctcgcgaaat
      661 aaggtccggc ccacccacta tttttccact taccgaatta cgaattaaaa cactgaaaac
      721 aattccagct tcagccacct tgcgacgatc tttgataacg actttactga tgtcgttacc
      781 gccctcgccc tcgataaaga ttcgattctc ttcaaaatga tcaacacgtt tgagagaatt
      841 tttggttaat tcaagaacat cgccgttgac ggcgattacg atattttctg cagccactcc
      901 gttttgttct gccaaacgag cgtgcttaac caggtggcga tactcgccgt gaaccgggat
      961 aaaatgacgc ggccgggtag cacgaagcat tttgcgcaat tcgtcggcgt tcgcgtgacc
     1021 ggaaacgtga atatcttcaa ccgattcgta caagacatca gctcccattt tgaacagaga
     1081 gttgatcatt cgtccgattg ctttttcgtt cccaggaata aacttgctcg acatgattac
     1141 agtatcacca ggaccaacgc taactgatcg gtgttcatcg ctcgcaattc gtgcaagtgc
     1201 cgatctaaat tctccttggc tgcccgtcga cagcacaatg actttgttgc gtgggaattg
     1261 ttcgatttcg tcgactccaa ttaacacgga tcgagcgtgt tcgagatagc cagcctcttg
     1321 ggcgagccta acattttgtt ccatgctgcg tccgagtaag gccactttgt gtccaaaatg
     1381 atcggctagt tccataacct gaccgatgcg cgcaatattc gaagcgaaaa ccgatacaat
     1441 cgttaggccg gtcggttccg cgaatagttc tttgaatttg cgataaatgt ttacttctga
     1501 aagagagtgt ccatctcgtt cgacgtttgt ggaatcactc atgagcaaaa ccacccccgc
     1561 gtcaccggct tttttaaag
//
LOCUS       DAOMXO010000600         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_46460_length_1579_cov_4.275591, whole genome shotgun sequence.
ACCESSION   DAOMXO010000600 DAOMXO010000000
VERSION     DAOMXO010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46460_length_1579_cov_4.275591"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="PLH57_10745"
     CDS             <1..1541
                     /locus_tag="PLH57_10745"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRK03130.1"
                     /translation="REKYIEEVCAMIQEKLKEYDLHGTVVGRPKHFHSIYKKMERRKI
                     DFEQIHDLIAFRILLDNITECYKALGVIHATYKPVPGRFKDYIAIPKANKYQSLHSTV
                     IGPHGERIEIQIRTNDMHQVAEHGIAAHWKYKEGRFESQDTNAVEWVNRLLEWHKDLS
                     DPNEFLETVKIDLFAEDVYVFTPNGDVMEFPFGSSPLDFAYAVHTDVGNRCMGAKVNG
                     KIVPLKYRLKSGDTVEILTSTTQTPSKDWLKLVKTSKAKAKIRAYIKIQERSRALALG
                     KEMLEKELRLYSYTVQKLLKLPQLEEFLRRKGTKTLDEVLIYVGYGRMQPQTISQAVL
                     PPEVLSTPKPEPKADDSVLKKIFDNAKKRTENKNAVTVENIDNILIRFARCCNPIPGD
                     SIVGFVSRGRGVTVHTVACPKALDTESDRKIDVTWNLQVPIKRHVRIRVLSHDTAGIL
                     ANMSQAISGCGVNISQANIRTTKDRKAVSMFEVEVANTEELAKVLSALEARKGVINVE
                     RVKS"
BASE COUNT          476 a          407 c          346 g          350 t
ORIGIN      
        1 aacgcgaaaa atacattgaa gaagtttgcg cgatgatcca ggaaaaacta aaagaatacg
       61 atctccacgg cacggtagtg ggccgcccaa aacattttca ttccatatat aaaaaaatgg
      121 agcgtcgtaa gattgatttt gagcaaattc acgatctcat cgcgttccgt attttgctcg
      181 acaatattac cgaatgttac aaagcgctcg gcgtaattca tgcgacctac aaacccgtac
      241 caggccgatt taaggattac atcgccattc ccaaggccaa caaataccag tcgcttcaca
      301 gcaccgtgat tggcccccat ggcgaacgca tcgaaatcca aattcgaacc aacgacatgc
      361 accaagttgc agaacacggt attgcggccc actggaaata caaagaaggg cgttttgaat
      421 cccaagacac caacgctgta gaatgggtca atcgattgct cgaatggcac aaggaccttt
      481 cagaccccaa cgagtttctt gaaaccgtga agatcgattt atttgcggaa gacgtctatg
      541 tcttcacgcc aaatggcgac gtcatggagt ttccatttgg tagtagcccg ctcgatttcg
      601 cgtacgcagt acatactgac gtcggaaatc ggtgcatggg cgccaaagtg aatggcaaaa
      661 ttgttccact caaataccga ctaaaatcgg gcgacaccgt ggaaattctc acctcgacta
      721 cgcaaactcc cagcaaagac tggctcaaat tagttaaaac cagcaaagct aaggccaaaa
      781 ttcgcgcata cattaagatt caagaacgtt cacgcgcgct ggcactcggc aaagaaatgt
      841 tggaaaaaga actgcgtctg tacagctaca cggttcagaa acttcttaag ttgccacaac
      901 ttgaagaatt tcttcgacgc aaaggtacta aaacgctcga cgaggttctt atttatgttg
      961 gttacgggcg catgcaacct caaacaattt ctcaggcagt cttgccgccc gaggtcttat
     1021 cgacaccgaa gcccgaaccg aaagctgacg atagcgtact caaaaagatt ttcgacaacg
     1081 caaaaaagcg taccgagaat aaaaacgcag tcacagtcga gaacatcgac aacatcttga
     1141 ttcgattcgc tcgctgctgc aaccccatac ccggcgactc aatcgttggt ttcgtatcac
     1201 gaggtcgcgg cgtaacggtg cacaccgtcg cttgccccaa agctcttgat accgaatcag
     1261 atcgcaagat cgacgtcact tggaatttac aagttccgat taaacgccac gttcgcattc
     1321 gagtactttc tcacgacact gcaggcattt tggcaaatat gtcgcaggca atttccggat
     1381 gcggagtcaa catttctcag gcaaacatac gcacaaccaa agaccgcaag gcggtgtcca
     1441 tgtttgaagt cgaagtggcc aacaccgaag agctcgcaaa ggtactatcc gcgctcgaag
     1501 ctcgaaaagg cgttatcaac gtcgagcgtg ttaaatccta acgactattt tttccactca
     1561 tcgagcattg ttcgcaggg
//
LOCUS       DAOMXO010000601         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_46645_length_1574_cov_5.405530, whole genome shotgun sequence.
ACCESSION   DAOMXO010000601 DAOMXO010000000
VERSION     DAOMXO010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46645_length_1574_cov_5.405530"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1098)
                     /locus_tag="PLH57_10750"
     CDS             complement(49..1098)
                     /locus_tag="PLH57_10750"
                     /inference="COORDINATES: protein motif:HMM:NF016822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HRK03131.1"
                     /translation="MPLIILREAKESDIPGLEVLSKIAAERHPGFLNISNDQDQWREK
                     IRRSTDSFSDKVANKMDAKYLFVAEDVATKRILGVSMVAAQHGTPDSPHFYFEVGSDQ
                     KYSQTISTGFIHGTLKFKWDTDGPTEIGGLLVDPEKRNSDERVGRQISFVRFLYIAMH
                     KAKFKRRILAELLPPLTKKGKSPLWEAVGRRFTNMDYTEADILSSKNKEFIFSLFPSE
                     KIYITFLSAEARNAIGKVSKETEPVMHMLKKIGFQYRNQVDPFDGGPHVSANVDDVLP
                     IRQMVQVNYVGGSAETQPNTGLRQGLVTPKGVTPFRSAHVEALVKDGKIWFSAPTADL
                     IGVRPGDAVHFMPYY"
     gene            complement(1089..>1574)
                     /locus_tag="PLH57_10755"
     CDS             complement(1089..>1574)
                     /locus_tag="PLH57_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRK03132.1"
                     /translation="DFLTAQDETFNPKTSTINFGKIVCRPGAVDLSFDIRLLPEVNPD
                     EFDSKLKGAIQGVNARYPNLTTKVSRVRFNPALNTSLESELVRVARDSQQLAGIPEKV
                     DKKATSTEAAQYYQAGFDAIVFGPGLSHGNSHSPNEYNIVDHLDKAVHFYDRMIERFC
                     L"
BASE COUNT          395 a          378 c          333 g          468 t
ORIGIN      
        1 aacttcgaat agttgcgttt tgcgctcatc gtacctccgc gatctttttc aatagtacgg
       61 cataaaatga acggcgtcac cagggcgcac cccgatcaaa tctgccgtag gagccgagaa
      121 ccaaattttt ccgtccttca ccaaggcttc gacatgtgct gacctgaacg gagtcactcc
      181 ttttggggtg actagaccct ggcgcagacc ggtattgggc tgcgtttcgg cggaaccgcc
      241 cacgtagttc acctgaacca tctggcgaat aggcaaaacg tcgtccacgt tcgctgaaac
      301 atgaggccca ccatcgaatg ggtctacttg attgcgatac tgaaaaccga ttttctttag
      361 catatgcata acaggttcgg tttctttgct cactttacca atcgcatttc gggcttctgc
      421 agataaaaaa gtgatataaa ttttctctga gggaaataaa gaaaagataa actctttgtt
      481 tttggagctc agaatatcgg cttctgtgta atccatattc gtaaatcttc gtccgaccgc
      541 ttcccacagt ggtgattttc cttttttcgt aagcgggggc agtaactccg cgagaattct
      601 tctcttaaac ttggccttat gcattgctat ataaagaaac cttacaaagg atatttggcg
      661 tccaactctt tcatcggaat ttcgtttttc cggatctacc agcagtcccc caatttcggt
      721 tggaccatcg gtgtcccatt tgaattttaa agttccatga ataaagcctg tagatatggt
      781 ttggctgtat ttttggtcgc ttcctacttc gaaataaaag tggggggaat caggagttcc
      841 gtgctgagct gcgaccatcg acacacccaa aattcgcttc gtagcaacgt cttccgcaac
      901 aaacaaatat ttcgcatcca ttttatttgc gactttgtca gaaaacgaat cggtcgacct
      961 tcgaattttc tcacgccatt gatcttggtc attcgaaata tttagaaacc ccggatgcct
     1021 ttcggccgcg attttcgata atacttctag accggggatg tctgactctt tggcttcacg
     1081 cagaataatc aaaggcaaaa cctctcaatc attcggtcat agaagtgcac cgccttatct
     1141 agatgatcta cgatattgta ctcatttggc gagtggctgt tgccatgact taatccggga
     1201 ccgaatacta tagcgtcgaa acccgcctga taatattgtg ccgcctcagt actggtagct
     1261 tttttatcta ctttttctgg aattcccgcg agttgctgtg aatctcgcgc cacgcgcacc
     1321 aattcgctct cgagtgaagt attaagtgcg ggattaaatc gcacacggct aactttggtc
     1381 gtgagattag ggtatcttgc gtttactccc tgaatggcgc ccttcagttt tgaatcaaat
     1441 tcatctggat taacttctgg caacaatcga atatcaaaac ttaaatctac tgctcccggc
     1501 cgacacacta ttttaccaaa atttatggtc gaagtttttg gattaaaggt ttcatcctgg
     1561 gccgtgagga aatc
//
LOCUS       DAOMXO010000602         1571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_46779_length_1571_cov_3.717018, whole genome shotgun sequence.
ACCESSION   DAOMXO010000602 DAOMXO010000000
VERSION     DAOMXO010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46779_length_1571_cov_3.717018"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLH57_10760"
     CDS             complement(<1..441)
                     /locus_tag="PLH57_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03133.1"
                     /translation="MLRKPRFLIRTRTLILGFALAAFGVTVPLLLKKDVARLASLYEL
                     IVDSEAFTEQSFELDATILLLTLELSEKSDNAKETLEQVNQQIRTLEDRRLPPSAMPG
                     ARQLRETFALWRTTTLAAVFSGERYTPMSDALAAQIRAINEELRM"
     gene            complement(431..814)
                     /locus_tag="PLH57_10765"
     CDS             complement(431..814)
                     /locus_tag="PLH57_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK03134.1"
                     /translation="MRILLAEDDTSIQRIASLALERVGGHQVTTVNNGRETLESLKHQ
                     TFDLILLDIMMPELDGFECCRILKGDAKTQAIPIIFLTARAQTQEIQKGLALGAAGYI
                     VKPFDPMTLPKQIEEILSGGQAHAS"
     gene            complement(817..1215)
                     /locus_tag="PLH57_10770"
     CDS             complement(817..1215)
                     /locus_tag="PLH57_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRK03135.1"
                     /translation="MNEIELLRQQYLAELQNKATTLGNLLQSILHDLGCKRAPNWKEL
                     EFMVHKFAGSSGTYGFGALSRVLSHLENALLSGALPRLKKNWAARYLQAWQQAFTLSV
                     KNAISEKKSPAALDNDLEELSQLLNAKEAA"
     gene            complement(1229..>1571)
                     /locus_tag="PLH57_10775"
     CDS             complement(1229..>1571)
                     /locus_tag="PLH57_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03136.1"
                     /translation="EATHHSEVQFAGGSTHAAASVIIPCIVSSVSEVGLVLRSKTYLD
                     RNLKIKIQSPVFSEIGIEPPFLRTSLCRSINDAEYDYEIAVSFIGLDEIAMRKIRRWI
                     TARAIAKRKIA"
BASE COUNT          361 a          372 c          405 g          433 t
ORIGIN      
        1 catgcgtagt tcttcgttaa tcgcccgaat ctgcgcagct aaagcatcgc tcatgggtgt
       61 gtatctttca ccagaaaata ctgctgccaa ggttgtggtt cgccacaacg cgaaagtttc
      121 gcgcaactgt cgggccccag gcattgcgct cgggggtaag cgccggtctt ctaaagtgcg
      181 aatttgttga tttacttgtt cgagagtttc ttttgcgttg tcgctttttt ccgagagctc
      241 cagagtaagc aataaaatcg ttgcgtcgag ttcgaaagac tgttccgtaa atgcttcgct
      301 gtcgacaatc aattcgtaga gcgatgcgag tcgggctacg tcttttttta aaagaagcgg
      361 caccgtcaca ccgaacgccg ccagtgcaaa tcctaagatc aaagtccgcg tgcgaattaa
      421 aaaacgcggt ttacgaagca tgcgcctgtc ccccgcttaa gatctcttcg atttgctttg
      481 gtagagtcat tggatcgaac ggttttacga tgtagccggc tgcgccaagt gcgagtccct
      541 tttgaatctc ctgggtttgg gctcgggcgg tcaaaaaaat aatgggaatc gcttgagtct
      601 tggcatcgcc tttaagaata cggcaacatt cgaacccgtc gagttcaggc atcatgatgt
      661 cgagtagaat taagtcaaaa gtctggtgtt tcaaggattc gagtgtttct cgtccgttgt
      721 taacagtggt tacttgatgg ccgccgacac gttcgagcgc taggcttgcg attcgttgta
      781 tcgacgtgtc gtcttctgcc aatagaattc gcatgactat gcggcctctt tggcgttgag
      841 aagctgacta agttcttcga ggtcgttatc cagcgcggcg ggagattttt tttcgctaat
      901 cgcatttttt acgctcaggg tgaacgcttg ttgccatgct tgaagatagc gggcagccca
      961 gtttttcttt aagcgtggta aggcgccaga aagaagcgcg ttttcaaggt gacttaagac
     1021 ccgagatagg gcgccgaaac cataggtgcc cgacgatcca gcgaatttat gaaccataaa
     1081 ctcgagttcc ttccaattgg gggctcgctt gcatcctaaa tcgtgcaaaa tcgactgcaa
     1141 caagttgcca agggtcgtgg ccttgttctg aagttccgcg agatactgct ggcgtagcaa
     1201 ttcgatttcg ttcattgagg tgtcctctct atgctatctt tcgtttggca atggcgcgcg
     1261 ccgtgatcca ccgccgaatc ttacgcatcg caatttcgtc gagtccaata aatgaaacgg
     1321 caatttcgta gtcgtattct gcgtcgttta tcgaacgaca gagactcgtt ctcagaaaag
     1381 gtggttcaat tccgatttcg gaaaataccg gcgactgaat ttttattttt aagttgcggt
     1441 cgagataggt tttgcttcgc aaaaccagtc ccacttcgga aaccgacgaa acaatgcaag
     1501 gaataatgac cgaggctgcc gcgtgggtac tgcccccagc aaattgcacc tcactgtggt
     1561 gcgtagcctc g
//
LOCUS       DAOMXO010000603         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_46828_length_1570_cov_3.973597, whole genome shotgun sequence.
ACCESSION   DAOMXO010000603 DAOMXO010000000
VERSION     DAOMXO010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46828_length_1570_cov_3.973597"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1152)
                     /locus_tag="PLH57_10780"
     CDS             complement(121..1152)
                     /locus_tag="PLH57_10780"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRK03137.1"
                     /translation="MKEYKNWSFVSDSSKTIEVEKFLRDHCGLQPRAILKRKLREKFV
                     LVNGRIADRLTLVRPKDRVDVLRGDDLYIAAEGQKIKPNPKIDCDVIYECSDYVVVNK
                     TAGQHSHPIRTSETDTLLNALVARYPECAEEGRIGRPLEGLLVHRLDEGTSGLIVCAR
                     TKSAFKQLQTLWAARSVTKIYTAWLRGTIVTAGTVELKLEHDPKSKKRMRVARASSKD
                     GWTTSTSIYPIAHENDHTLVLLQIHTGVTHQIRATMAHLGVPVAGDPIYGEPQSKATR
                     SATVKIRGLPHEELHRFHLLKNQLKPSRWRSPKRTSKNRGFSLHATFLALPLPYDQQW
                     FCPPIHWDH"
     gene            complement(1149..>1570)
                     /locus_tag="PLH57_10785"
     CDS             complement(1149..>1570)
                     /locus_tag="PLH57_10785"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRK03138.1"
                     /translation="SIQAIEAHLRSLPEKELHETLALTPSYIFFVISDKPALTTIGVP
                     AVAGRSVAVDAKLFPKGALAFLRGQKPIFQDDQAVFPESFEAFSRLVVDHDTGGAITG
                     PARLDLFWGSGKDAARHAGVIRHEGNLFYLAPKVAKP"
BASE COUNT          352 a          374 c          414 g          430 t
ORIGIN      
        1 atgcgagtag agccacggca gataaatatt tgtaagacat agttctcctt atcttctggt
       61 gcttgcccgt accttatctc atcttgcgcg ttttttaact cggcaaatgt ttccggtgtt
      121 ttaatggtcc cagtgaatcg gcggacagaa ccactgctgg tcatagggca gcggaagcgc
      181 cagaaaggtt gcgtggagac tgaatccgcg gtttttactg gtgcgcttgg ggctgcgcca
      241 tcgactaggt tttaattgat ttttaagcaa atggaatcgg tgcaattcct cgtgcggtag
      301 tccccgaatt tttacagtcg ccgagcgagt agctttggac tggggttccc cgtagatcgg
      361 atcgcccgca accggaacgc ctaaatgggc catagttgcg cgaatttggt gggtgactcc
      421 ggtgtgaatt tgaagaagta caagagtgtg atcattttcg tgagcaatcg gatagatgct
      481 agtcgaggtg gtccatccgt ctttcgaact agctctcgcc actcgcatgc gtttttttga
      541 tttaggatcg tgttcaagtt taagttctac tgtgccagca gttacaattg ttccgcgcaa
      601 ccatgcggtg taaattttgg taaccgatcg agcggcccaa agtgtttgta actgtttaaa
      661 cgccgacttg gtgcgcgcgc atacgatgag tccggaggtg ccttcgtcta atctgtggac
      721 aagtaagcct tcaaggggcc gtccgatgcg cccttcttcg gcacattcgg gatagcgagc
      781 cactaaagcg ttgagaagtg tgtcggtttc ggaagtccgt atcgggtgcg agtgttgacc
      841 ggccgttttg ttaacaacca cgtagtcgct gcactcgtaa attacatcac agtcgatttt
      901 agggttgggt tttatttttt ggccttcggc agcgatgtac aaatcgtcgc cccgaagcac
      961 gtcgactcgg tccttcgggc gaacgagagt cagacgatcc gcaatgcgcc cattcaccaa
     1021 aacgaacttc tcacgcaact tgcgttttaa aatggctctg ggttgcagtc cgcagtggtc
     1081 gcgtaagaac ttttctactt caatggtttt ggatgagtca ctgacaaacg accagttctt
     1141 gtattccttc acggttttgc aaccttcggt gccaagtaaa aaagattacc ctcgtggcga
     1201 atgactcccg cgtggcgggc agcatctttt cccgaacccc aaaataaatc gagccgcgcg
     1261 ggcccagtga tggctcctcc ggtgtcgtgg tctactacta gtctcgaaaa agcttcaaag
     1321 ctctccggaa aaacagcttg gtcgtcttga aatattggtt tctggccgcg gagaaaagct
     1381 agtgctcctt taggaaacaa ttttgcgtct accgcaacgg atcggccagc caccgctggt
     1441 actccaatcg tagttagtgc aggtttatcg ctaattacga aaaatatata gctaggggtc
     1501 agcgccaaag tttcgtgcaa ttctttttct ggcagagacc tcaggtgggc ttcaatggct
     1561 tgaatcgaca
//
LOCUS       DAOMXO010000604         1568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_46916_length_1568_cov_3.407799, whole genome shotgun sequence.
ACCESSION   DAOMXO010000604 DAOMXO010000000
VERSION     DAOMXO010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46916_length_1568_cov_3.407799"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PLH57_10790"
     CDS             complement(<1..267)
                     /locus_tag="PLH57_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03139.1"
                     /translation="MLVKILKKVTFTLLVLNVAFLFASCASAPKPIDSEIGDSGLSQE
                     SIDAIRAIEREIQATDDHIQEKHEKFKIPIEVNHLVLKWVHFKTR"
     gene            complement(275..>1568)
                     /locus_tag="PLH57_10795"
     CDS             complement(275..>1568)
                     /locus_tag="PLH57_10795"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRK03140.1"
                     /translation="VRVHRFNSLCAVVRELAFESMTQEQRDRILMLVKAWKNTKKTAP
                     FGFLNQIDPFYFANEKKFFVLELHGRILAFLAAVPVFALEGYFFSEYMRAAESRVGTT
                     ELLFIETMRLLKKAGYKEVRLGTCLFSNLRVNSESNLRQRVMSFAMGLAFRHLRYPLN
                     FKSIYQFKAKFEPTRWEPVYLASKSKVGIRVLFDVLRVHFKELNLGARLQGYFAQGIN
                     TRALPKTFGEFLYRSKITFIFSAIFVVLHLWRTFSTTGLAFFQKNGYSVSGYSASGHF
                     WSPLFHNGHTHFFGDLSTFLIFGTATEVFLGSWFAASVIAFGLWASNPQAVYIVEKLI
                     GPNIDAATLARFRSETDYGSSNAIYAMVGALSGLLKRPMLLMLPFILNGLFLCIALKS
                     ILSIHHLTALFTGFVICKLLHIVQKKFPTSVLEQSKTE"
BASE COUNT          485 a          355 c          306 g          422 t
ORIGIN      
        1 tctcgttttg aagtgtaccc acttcaaaac gagatgattt acctcgatcg gtattttaaa
       61 tttttcgtgt ttttcttgaa tatgatcatc ggtggcttga atctcgcgtt caatagctcg
      121 aatcgcatcg atcgactctt gagacaaacc gctatcaccg atttcgctat caatgggctt
      181 tggggcgctc gcgcatgaag caaaaagaaa cgccacattc aaaaccagca aagtaaatgt
      241 cacttttttt aagattttaa cgagcacaat agatctattc tgtcttactt tgctccaaaa
      301 ccgaagtggg aaattttttc tggacaatgt gcaatagctt gcaaattaca aaaccagtaa
      361 atagtgccgt tagatgatga atactcaaaa tactttttag cgcaatacag agaaataaac
      421 cgtttaaaat aaagggcaac atgagcaaca ttggccgctt aagtagacca cttagcgcac
      481 ctaccatggc atatatcgca ttcgaggaac cataatctgt ttcagatcta aaccgggcca
      541 atgtggcagc atctatgttc ggcccaatta acttttcaac tatgtaaacc gcttgtggat
      601 ttgacgccca aagcccaaac gcaattacac tggcggcgaa ccatgacccc aaaaatactt
      661 ccgtagcagt gccgaaaatt aaaaaggtcg ataaatcacc gaaaaagtgc gtgtgaccgt
      721 tatggaataa tggactccaa aaatgtccgc tagcggaata gcccgagaca ctatacccat
      781 tcttttgaaa aaacgccaaa ccggtggtcg aaaatgtacg ccacaaatgc aataccacga
      841 aaatagccga aaagatgaat gtgattttac tgcggtaaag gaactcacca aaagtttttg
      901 ggagcgctcg tgtgtttatt ccctgtgcaa agtatccttg aagtcgggcc cctaaattta
      961 gttccttaaa gtgtactctt agaacgtcaa acaagactct tattccaacc ttgctcttgc
     1021 tcgctagata aacgggctcc caacgagttg gttcgaactt agccttaaat tgataaatgg
     1081 acttgaagtt caaaggatat cgcaggtgcc taaaggctaa tcccatggca aagctcatta
     1141 cgcgctgacg cagattgctt tctgaattca ctctaagatt tgaaaacaag caggttccta
     1201 gtcttacttc tttgtaaccc gcctttttta gcagacgcat tgtttcaata aagagtagtt
     1261 ctgtagtacc gacacggctt tcagcggctc tcatgtattc ggaaaaaaaa taaccttcca
     1321 atgcaaacac gggcactgct gccaaaaaag cgagaattcg cccgtgtaac tctaagacaa
     1381 aaaatttttt ttcattagcg aaataaaagg ggtcgatttg gttcaagaaa ccaaacggag
     1441 cggttttttt tgtgtttttc caagccttta caagcattaa aatacgatcg cgctgctcct
     1501 gtgtcatgct ctcgaatgca agctctctca ccaccgcaca caaggagttg aatcgatgta
     1561 cccgcacc
//
LOCUS       DAOMXO010000605         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_47153_length_1563_cov_3.806366, whole genome shotgun sequence.
ACCESSION   DAOMXO010000605 DAOMXO010000000
VERSION     DAOMXO010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47153_length_1563_cov_3.806366"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PLH57_10800"
     CDS             <1..1322
                     /locus_tag="PLH57_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRK03141.1"
                     /translation="SAKEGVDTLVLDAGDFSEGSQFYLADRGEQTWKVMDAMGYDAVT
                     IGNHDWLMGQRELNRLIGNVKPSFNLLGANFVAEEKYQNIRNHLRPFVEFERNGLRVA
                     VIGLSTDLFVWAWRAGKNALLRPETVAKNLLPKLTANNDLVIALTHIGTSGDRWLVRS
                     TKGIDIVIGGHSHTTLSKPVYVRDQRGHSVPIVQTGAHGHLIGSLLVDVVPGRSVKVV
                     KYELIPVDPVDSGKDSHIEGLVHDARRELENDYGRDWLYEVLAHSEVPLERPMNGPTV
                     WGKIVDSAIRERAKAHLSINQGALYGINLPSGPITRETLFEFYPRMFEFSRKYGWTIW
                     TAKAQGWLLEAALKLALENGSYLNTSGLTYDEKIVDGKRRISNMKIGGKPIERFKNYK
                     LALPEGIARGSKSLNKYLRYILQKPTDTKVPIWTAVADKVRRMGTIR"
     gene            complement(1319..>1563)
                     /locus_tag="PLH57_10805"
     CDS             complement(1319..>1563)
                     /locus_tag="PLH57_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03142.1"
                     /translation="SSGYLISWGRWPYWLQQKIFFGLIAAAINFFCIFCVLQRKRYDA
                     VNSSKRVFYDRCVRLSILGVPFAALALWYASQRYFS"
BASE COUNT          451 a          335 c          403 g          374 t
ORIGIN      
        1 aatcagcaaa agaaggggtc gacacgctcg tactcgatgc tggtgatttt agtgagggat
       61 cgcagtttta tctcgcggat cgcggagaac aaacctggaa agtcatggat gcgatggggt
      121 atgacgctgt aactattggc aatcacgatt ggttgatggg gcaacgcgaa ctcaatcgtt
      181 tgataggaaa tgttaaaccg agtttcaatt tattaggtgc taactttgta gccgaagaaa
      241 aatatcaaaa tattcgcaat caccttcgac catttgtcga gttcgaacgc aacggattaa
      301 gagtggctgt gattggttta tcaaccgatt tgttcgtgtg ggcctggcgt gcagggaaaa
      361 atgcactatt gagacctgaa acggtcgcaa aaaatcttct gccgaaactc actgctaaca
      421 atgatttggt catagcactt actcacatcg gaactagcgg tgatcgttgg ttagtaaggt
      481 cgaccaaggg tatagatatt gtcatcggag gtcacagtca cacaacactt tcgaagcccg
      541 tatatgtgcg ggaccagcgc ggacactcag tgccgatcgt acaaacaggc gctcatggcc
      601 acttaattgg aagtctttta gtggatgtag tgcccggtcg ttcggtaaaa gttgttaagt
      661 acgaattgat tccggttgat cccgttgatt ctggcaaaga ttcacacatt gaaggtttgg
      721 ttcacgatgc acggcgagag ttggaaaacg attacggacg agattggctt tacgaagtac
      781 tagcgcattc ggaagtaccc ttggagcgtc cgatgaatgg ccccactgtg tggggaaaaa
      841 tcgtagacag cgctattcgg gagcgagcga aagctcatct gtcgatcaac caaggggctt
      901 tgtacgggat caatctgcct tccggaccaa taactcgcga aacgcttttc gagttttacc
      961 cacgaatgtt tgagttttca cggaagtacg gatggactat ctggactgcg aaagcccagg
     1021 gatggctgct cgaggcggca ttaaaactgg cccttgaaaa cggaagttat ctaaatacct
     1081 ccggcctaac ttacgacgaa aagatcgtcg acggaaaaag gcgcatttcc aatatgaaaa
     1141 tcgggggcaa accgatcgag agatttaaaa attacaaact cgcattgccc gagggtatag
     1201 ctcgcggatc gaaatcgctt aacaaatatt tgcgctatat tcttcaaaaa cctaccgata
     1261 cgaaggtgcc gatttggaca gccgtggccg acaaagtgcg acgtatgggt acgattcgct
     1321 agctaaaata gcgttgggaa gcgtaccaaa gcgcgagggc tgcgaagggt actcctagaa
     1381 tcgaaagccg tacacaccgg tcatagaaga ctcttttcga tgaattaact gcgtcgtagc
     1441 gctttctttg caagacgcaa aaaatgcaga aaaaattaat agccgccgcg atcaaaccga
     1501 aaaatatttt ttgctgtaac cagtaaggcc accggcccca ggatatcaaa taaccgcttg
     1561 aag
//
LOCUS       DAOMXO010000606         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_47167_length_1563_cov_2.951592, whole genome shotgun sequence.
ACCESSION   DAOMXO010000606 DAOMXO010000000
VERSION     DAOMXO010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47167_length_1563_cov_2.951592"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="PLH57_10810"
     CDS             complement(<1..1333)
                     /locus_tag="PLH57_10810"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRK03143.1"
                     /translation="MKRYWNTTLVLTFMSVLIPSLYSLAEARQLVQEQSALPLTTFNV
                     TFRVGSVDDPAGKAGMAAVVAELVRNGGVAIWKKQPARSRLEFEKLLFPLAAVIETSV
                     QKEQTSFRVTTAASNSIKVFKLLTQLIVSPGLNISEFERIKGETLDYLKEQFPREDQE
                     ELGKAALDLAIYGKGHPYAHPVAGLSADVQTITHEEAVAFYKKYFTQKRIIVAAAGRV
                     TDELKRAMLELEAALPAGDSSSTKIPPAPVNKGISVTIVEGPFDGTGVHLGHALPINR
                     RDERFAQLVLAANGFGKHRSFVGRLMHVVREVRGLNYGTYSYVEDFPYGGRLLTEPLQ
                     VARQIQAFTVWGRPTPHEDGCFLTRQIFRELQSLKTQGLTTEEFTRTKSHLQGALPIL
                     GSPLERRLGYAVDSVFYGTPSNYLAELVTRLSRASNEAVKNTIQKYIDTDNL"
     gene            complement(1330..>1563)
                     /locus_tag="PLH57_10815"
     CDS             complement(1330..>1563)
                     /locus_tag="PLH57_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03144.1"
                     /translation="KNRLMNERNVGWFSSPDALASRIAWYSTIDPPSLAGFSVLDRVM
                     NSIKTLAPNQIQSFAQTHLVEAKRNIVILRGNE"
BASE COUNT          369 a          388 c          403 g          403 t
ORIGIN      
        1 gtagattgtc ggtgtctata tatttttgaa ttgtattttt aacagcctcg tttgatgcgc
       61 gtgaaagacg cgttaccaac tccgccaaat agttagacgg tgtgccatag aataccgaat
      121 ctacggcgta gccgagacgt cgttcgagcg gacttcccaa tatgggcaac gccccctgaa
      181 gatgactttt ggttctcgtg aactcttcag tagtcagccc ctgagttttc agcgactgaa
      241 gttcacgaaa aatttggcgt gtcaaaaaac agccgtcctc atgaggagta ggacgtcccc
      301 aaaccgtgaa cgcttgaatt tgccgtgcta cctgcaaggg ttctgtcaga agtcggccgc
      361 cgtacggaaa gtcttccacg taggaatagg ttccgtaatt taagccccgg acttcgcgca
      421 ccacatgcat gagtcggccc acgaagctgc ggtgttttcc gaaaccgttt gccgccaata
      481 caagctgcgc aaaccgttca tcgcgccgat tgattggaag tgcgtgacca aggtgtactc
      541 ctgtgccatc aaaaggaccc tcgacgatgg taaccgaaat acccttattc actggggcag
      601 gtggaatttt agtggaagaa gaatccccag cggggagcgc ggcctctagt tctaacattg
      661 cgcgttttag ctcgtctgtg actcgtcccg ctgcggctac gataattcgt ttctgggtga
      721 aatatttttt gtaaaaagcc acggcctctt cgtgagtgat cgtctgaacg tcggcgctta
      781 atccggcaac cgggtgggcg taggggtgac ccttgccata gatcgcaaga tctagtgccg
      841 cctttccgag ttcttcttga tcttcgcggg gaaactgttc ttttaagtag tcgagcgttt
      901 cgcctttaat gcgttcgaat tcgctaatat tgagtccggg actaactatt aattgagtga
      961 gcagcttgaa cactttgatc gaattgctgg cagcggtcgt gacccgaaag ctcgtctgct
     1021 ctttttgaac ggaagtttcg atcacggctg ccagtgggaa cagtagtttt tcaaactcca
     1081 agcgagatcg cgcaggttgc tttttccaaa tcgccacgcc gccattgcgc acaagctctg
     1141 ccactacggc agccatgccg gctttaccgg caggatcatc gaccgaacct acgcgaaacg
     1201 ttacgttaaa tgtcgtcagt ggtagtgcac tttgttcctg cactaactgg cgagcctccg
     1261 ctaatgagta cagcgacgga attaacaccg acatgaaggt tagaacgaga gttgtattcc
     1321 agtaacgttt cattcgttcc cccggagtat gacaatgttt cttttagctt caaccaggtg
     1381 ggtctgcgcg aaagactgta tctgatttgg cgccaatgtt ttaatggagt tcatcactcg
     1441 atcaagaacg ctaaaccccg ccagtgaagg agggtcgatc gtagagtacc aagcgatgcg
     1501 agacgcaagg gcatcaggag agctaaacca accgacgttg cgttcgttca taagacggtt
     1561 ttt
//
LOCUS       DAOMXO010000607         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_47173_length_1562_cov_7.562707, whole genome shotgun sequence.
ACCESSION   DAOMXO010000607 DAOMXO010000000
VERSION     DAOMXO010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47173_length_1562_cov_7.562707"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..443)
                     /gene="fliS"
                     /locus_tag="PLH57_10820"
     CDS             complement(51..443)
                     /gene="fliS"
                     /locus_tag="PLH57_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRK03145.1"
                     /translation="MSNNYGVNKYKQTSVTTASRGQVLLMLYEGAIRFTKLAIEGVKK
                     NDRVQKGTYIVKVQDIVNELSLSLNHEVGGDISKELDRLYNYIIEQLTEANVKNDAKP
                     LEVILKLLETLHDGWKVAVDQYSKTEEK"
     gene            complement(459..>1562)
                     /gene="fliD"
                     /locus_tag="PLH57_10825"
     CDS             complement(459..>1562)
                     /gene="fliD"
                     /locus_tag="PLH57_10825"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRK03146.1"
                     /translation="ETGEYQIEVTQLAGRHSMISNGYEDPTDEIGIGYFTYDLPSGET
                     KSVWVGPGNNTLQGLVRAINDERKLGVQASLVNDGSDSGTPWRVVIAGKKAGVDQDIV
                     YPDFYFLDGDFRFYADDERQAQNAIIKFNGFEIMSQGNKFELLPGVTLDLKQAKEDYE
                     FTLSIGEDIAKIAAKVRALVDSINAVLDFVVAQNKLTAESDTSRTLGGDTTLFTVQSR
                     IQTWVFEYFNVDYDDEDAYMRLGDIGITFQKEGNLKFDEDKFKKVLADNFDYVAELFA
                     GEGNFIEQVKEMTQSFLAPGIGVLGSREAGIRDRIRRIDQDIARKEMHIARKEQQLKR
                     QFSQLEGLVNSMQGQQGFLQQAFGNASAAMAGG"
BASE COUNT          369 a          380 c          335 g          478 t
ORIGIN      
        1 ttcgcaaaca gagattctta cgctctaaaa tttcgataag gacttccatg tcacttctcc
       61 tcagttttac tgtactggtc gacagccact ttccagccgt catgtagagt ttccagaagc
      121 ttcaagataa cttcaagggg tttcgcatca tttttgacat tggcctcagt aagttgctca
      181 attatgtaat tgtatagtcg atcgagctct ttactgatgt cgccgcccac ttcgtgattg
      241 agcgaaagcg aaagttcgtt cacaatgtcc tgcaccttaa ctatgtaggt gcccttctga
      301 acacggtcat tcttttttac gccttcgatt gcgagcttgg taaagcgaat agcgccctcg
      361 tagagcatga gcagtacttg cccgcgactt gcagtcgtta ctgaggtttg tttgtactta
      421 ttaaccccat aattgttact catcgttcgg tctccctgtt aaccaccggc cattgccgcg
      481 ctggcgtttc caaaagcttg ttgcaagaat ccttgttgcc cctgcataga atttaccagg
      541 ccttctagct gggaaaattg tctctttaat tgttgttcct tacgagctat gtgcatttct
      601 tttcgtgcaa tatcttgatc aatgcggcga attcgatctc gaataccagc ctcacgggaa
      661 ccaagtacac cgatgccggg agcgagaaag ctttgagtca tttcttttac ttgttcgatg
      721 aaattacctt cgccagcgaa gagttccgcg acataatcaa aattgtcggc gagtactttt
      781 ttaaatttgt cttcatcgaa ttttaaatta ccttcctttt gaaaggtaat tccgatgtct
      841 ccaagtcgca tataggcatc ttcatcgtcg taatcgacat taaaatattc aaatacccac
      901 gtctgaatcc gcgactgtac agtaaagagc gtggtgtcgc caccaagcgt gcggctagtg
      961 tccgattcgg cggtgagttt attctgagcc acaacgaaat ctagtaccgc atttatggaa
     1021 tcaaccagag ctcgcacttt agccgcgatc tttgcaatgt cttcaccgat acttagagta
     1081 aattcataat cttccttggc ttgtttgaga tctagagtta ctccgggcaa caattcgaat
     1141 ttgttgccct ggctcatgat ttcaaaacca ttaaatttga taatcgcgtt ttgcgcctgg
     1201 cgttcgtcgt ctgcatagaa gcgaaaatca ccatcgagga agtaaaaatc cggataaact
     1261 atgtcctgat cgacgccggc tttttttccg gcgattacta cgcgccaagg ggttccgcta
     1321 tcggaaccgt cgttaactag tgacgcttga acgcccagtt ttcgttcgtc gttaatagcc
     1381 cgaactaggc cttgcagggt gttattcccg gggccgaccc acacgctttt ggtttcaccg
     1441 ctcggtagat cgtaggtgaa atagccgatt cctatttcat cggtcggatc ttcataaccg
     1501 ttagaaatca tcgagtgtcg accggcgagt tgcgtgactt caatttggta ctcgcctgtt
     1561 tc
//
LOCUS       DAOMXO010000608         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_47183_length_1562_cov_4.550100, whole genome shotgun sequence.
ACCESSION   DAOMXO010000608 DAOMXO010000000
VERSION     DAOMXO010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47183_length_1562_cov_4.550100"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLH57_10830"
     CDS             complement(<1..917)
                     /locus_tag="PLH57_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRK03147.1"
                     /translation="MPGHIIAVVGGKGGVGKSVFAANLALAYLMELRQRPLLIDLDLG
                     SLGDQNLILGVRPQKTVVDASKIQGAVDLKALQSIMATTSTGMSFIAAPGDTMAAREL
                     DLDGVGRLLKAAVGIFPFVVVDCGSVVDATTVKVFEQATAIFTVTTQDVIVVNQTRRL
                     LGKIQEHLFPPDMVQIIVNRFSPNAIVNPQVIQKNLNKTIFAAVPEEGETCSGALARG
                     QPFVGSAPQATITRAFHDIIRRIQQTNLLVSLSNLKKPTGAAAKLANSVPNTVGAGAS
                     TTSSGGSSIFSAGKSTDAWTALKLRIHKG"
     gene            complement(946..>1562)
                     /locus_tag="PLH57_10835"
     CDS             complement(946..>1562)
                     /locus_tag="PLH57_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03148.1"
                     /translation="GSQGGVTTEGTGSLSGTIGNLFPKLNSAQNAGYARILEEAVLVV
                     KEGAKVALDRQTRIPVQSTNAQTGQINYQGETIGPKLSIVPNMQGEEDIELNIDFNYK
                     GLVGQSGGAPITQNHNYVTVLAVRNGQSAAVVNAISNVVTTNFNKDPPGGKVPENPLF
                     TLLRSKAFQKNKSQFVIFVTPQVIESASGGTEDIKAKYGLKRKQ"
BASE COUNT          374 a          409 c          352 g          427 t
ORIGIN      
        1 aatcccttgt gaatgcgtag cttaagcgcg gtccaagcgt cggttgattt tccggcggaa
       61 aaaattgaac taccgccgga cgacgtggtc gaagcacccg caccaacggt attcggaaca
      121 gagttggcca acttggcggc cgcaccggtt ggttttttta aattcgaaag cgaaaccaat
      181 aaatttgtct gttgaattcg acgtataatg tcgtggaagg ctctcgtaat agtagcctgg
      241 ggcgcagaac caacgaatgg ttgccctcgc gccaacgccc cggaacaggt ttcgccctct
      301 tccggcacag ctgcaaaaat tgttttattt aaatttttct gaataacctg cggattcact
      361 atcgcgtttg gcgagaagcg attcacaatt atttgaacca tatccggagg aaaaagatgt
      421 tcctgaattt ttccgagcaa tcgacgtgtt tggtttacta cgatcacatc ttgagtggta
      481 actgtgaaaa tcgcggtagc ttgttcgaac acttttacag ttgtagcatc tactactgaa
      541 ccgcagtcca ccaccacaaa cgggaaaatc cccacagccg ccttcagaag tcgcccgaca
      601 ccgtccaggt caagttcccg tgcggccata gtatctccgg gagccgcgat aaaactcata
      661 ccggtcgagg tcgtcgccat aatactctga agcgctttta gatcgactgc cccttgaatt
      721 ttactagcgt cgaccacagt tttctggggg cgcactccta gaattaaatt ttgatctccc
      781 aaacttccga gatcgagatc gatcaagaga ggtcgctgtc gcagttccat caaataggcg
      841 agcgcgagat tggcggcaaa tactgacttt cccacgcccc cctttccacc tacgactgca
      901 atgatatggc caggcattag ttcgccctcc tcattgcaaa agttttcatt gtttcctctt
      961 caagccatac ttcgctttta tatcttcggt tccaccagag gctgattcaa taacctgtgg
     1021 cgtaacaaaa attacgaact gactcttatt cttttgaaac gctttggatc gcagcaaagt
     1081 aaacaatgga ttctctggca ccttgccgcc tggaggatct ttattaaagt tggtcgtcac
     1141 tacattactg atcgcattca ccacggcggc actctgaccg ttacgtaccg ccagtaccgt
     1201 gacgtaattg tggttctggg tgatcggtgc accacccgac tgcccaacca aacctttgta
     1261 gttgaagtcg atatttagtt cgatgtcttc ttcgccttgc atgttgggaa caatgcttaa
     1321 ctttggtcct atagtttcgc cttggtagtt aatctgaccg gtttgcgcat tcgtactttg
     1381 cactggtatt cgcgtttggc gatcgagtgc gaccttggca ccctctttca ccacgagcac
     1441 tgcttcttcc aaaattctcg cgtagccagc attttgagct gaattcagct tgggaaacag
     1501 gttaccaata gttccagaaa gagatcctgt gccctcggtt gtcacaccac cttgggagcc
     1561 ct
//
LOCUS       DAOMXO010000609         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_47304_length_1559_cov_5.152926, whole genome shotgun sequence.
ACCESSION   DAOMXO010000609 DAOMXO010000000
VERSION     DAOMXO010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47304_length_1559_cov_5.152926"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="PLH57_10840"
     CDS             complement(<1..1311)
                     /locus_tag="PLH57_10840"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRK03149.1"
                     /translation="MKPAKHSHERKVYGLARMVLGYATWLTRYHRAILWISFSSFIVA
                     GYFSVKLYQDLRTDLEELLPETAQSVRDVRSAVRRIGGLNHLSIVLETTDSAAAIRLS
                     NDIAAELKKLPPELVARVQNSISAEKSFFEKYKALYVDLDDWKRLDQYVRDRIRYERK
                     GKSPFSLGLEDESQAPSFDFEGLRKKYEERAGAYSRFPGGYFQSRDGRHTVVLAFLPG
                     KVTDQQANQKLSAAAHTIVEKLAPKTYAPDMIVGFNGDVQNMVEEHEGLTEDLISSSI
                     IVTILCALVLYLYFRSFWAVYALCAALFAGVAWTFGLSYGIVGYLNANTAFMGAIVVG
                     NGINFGIIMAARYFELRRQMVTGSALIARSIAYTAQSTWTAAAAAGAAYLSLVLTDFR
                     GFNQFGLIGGMGMVLCWISSFLVLPAMTEDDGLKLLGGSPAIQAG"
     gene            complement(1308..>1559)
                     /locus_tag="PLH57_10845"
     CDS             complement(1308..>1559)
                     /locus_tag="PLH57_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03150.1"
                     /translation="DSRHGFLESDLECLAFLNDRKLRFGVIFTKLDKWKSPSAIKKAE
                     KDLLLLAERLGVEQVFFTSAEKNLGLGTVKGALVDWSRV"
BASE COUNT          437 a          428 c          329 g          365 t
ORIGIN      
        1 accggcttgt atagcagggc taccccccaa cagtttgagc ccgtcgtctt cggtcatggc
       61 gggcagcacc aaaaaactac tgatccaaca cagcaccata cccatgccgc caatcaagcc
      121 gaactgatta aaccctcgaa aatccgtcaa cacgagggac agatatgcag cgccggctgc
      181 ggcggccgca gtccaagtgc tttgagcagt gtaagcaatg ctcctagcaa tcaacgcgct
      241 acccgttacc atttggcggc gtagttcaaa atagcgcgcc gccattatga taccaaaatt
      301 gataccgttc cctacgacga tagctcccat aaaagcggtg ttggcgttta gataaccaac
      361 gatcccgtag gacaacccaa acgtccaggc tactcccgca aataacgcgg cacaaagcgc
      421 ataaactgcc caaaaagaac gaaaatagag atacaagacg agggcacaaa gaatggtcac
      481 gataatcgac gaggaaatga gatcttccgt caatccctcg tgttcctcaa ccatgttctg
      541 aacatctcca ttaaacccaa cgatcatgtc gggagcatag gtttttggtg ctaacttctc
      601 tacgatcgta tgagctgcgg ccgagagttt ctggttggcc tgttgatccg taacttttcc
      661 tggtaaaaac gccaacacca ccgtgtggcg accatcccgc gactgaaaat agccgccagg
      721 aaatctgcta taggctccgg cacgttcttc gtattttttt cttaaaccct cgaaatcaaa
      781 gctaggcgct tgcgattcgt cctccaaacc tagagaaaaa ggacttttac ctttgcgctc
      841 gtagcgaatc cgatctcgca cgtactggtc gaggcgcttc cagtcatcga gatcgacgta
      901 aagtgcctta tatttttcga aaaaactttt ctcggcacta atcgagtttt gaactctagc
      961 aaccaattcg ggtggcaatt tttttagttc ggcggcgata tcgttagaaa gtcgaatagc
     1021 agcggcggag tctgtcgttt ctagtacaat cgaaaggtgg tttaacccac cgattcgtcg
     1081 caccgccgaa cgcacatcgc gcacactctg tgcggtttct ggcaataact cctcaagatc
     1141 tgtgcgcaga tcttggtaca attttaccga aaaataaccc gccacaataa aactcgaaaa
     1201 agaaatccag aggatagctc tgtggtagcg agtcaaccaa gtcgcatacc ctaacaccat
     1261 tcgcgccagg ccatacactt tgcgttcgtg agagtgttta gccggtttca cacccgactc
     1321 caatcgacca gtgcgccctt cacagtaccc agtccaagat ttttttcagc cgaggtaaaa
     1381 aacacttgtt ccactcctaa acgctcagca agtaacaaga gatctttttc agccttctta
     1441 attgcacttg gtgacttcca cttgtcgagt ttcgtaaaaa tcacgccaaa ccgcagttta
     1501 cgatcgttca aaaacgcaag acactctaaa tcgctttcta aaaaaccatg tcgagaatc
//
LOCUS       DAOMXO010000610         1553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_47572_length_1553_cov_4.412550, whole genome shotgun sequence.
ACCESSION   DAOMXO010000610 DAOMXO010000000
VERSION     DAOMXO010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47572_length_1553_cov_4.412550"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLH57_10850"
     CDS             complement(<1..759)
                     /locus_tag="PLH57_10850"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRK03151.1"
                     /translation="MISLDCKLERTLDDDTWDLVSGLLYQFHSQGLEEGATPAPPDDK
                     SMLAGNEFELALEPGILSPPREFKAYFENRRDAERAERAVTPILAAYNPRFCIEEVSV
                     HDYSELWRQSVRPMEVAPNWYIRAPWHPPLSQLNLGRPLNELIVEPGMAFGTGSHETT
                     QSCLTLLSRVLDGGLVSKMHALDFGCGSGILAIALKRLGIEHVTAIDIDPLAIDATHA
                     NAALNGVSVTATTSSPDASPAQYDGIVANILRNTL"
     gene            complement(785..1384)
                     /locus_tag="PLH57_10855"
     CDS             complement(785..1384)
                     /locus_tag="PLH57_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRK03152.1"
                     /translation="MRSSIKNRRRRHRIKRDFDLQLTSMMDMLIIMVVFLLKSYATNS
                     VAFATSSNIKLPSSTAEEIPADSINLVVEPTGIMFDNEKVMDFANLPVIPSPAPAGFT
                     FTPTYEIESRHLADYGRRILPLYDAMVKSRQKAELMMSKAVVVDPTTGERSTPKFQGV
                     VIIHADKSVKFDILRKIMYTAGSAEFKIFKLVAEKKEEA"
BASE COUNT          364 a          358 c          429 g          402 t
ORIGIN      
        1 aagagtgttt ctgaggatgt tggcaacgat tccgtcgtat tgcgcgggcg aggcatcggg
       61 gctactcgtg gtggcggtca ccgagacacc gttgagggct gcattggcgt gggtcgcatc
      121 gatggcgagc gggtcaatgt cgattgcggt gacgtgctca attcctaatc gtttaagggc
      181 gattgccaga attccggaac cacacccgaa atctagtgcg tgcattttgg aaactagacc
      241 tccgtctaac acgcgactaa gaagagtgag gcaggactga gtagtttcgt gagagccagt
      301 accgaaagcc attcctggct cgacgatcaa ttcattcaac ggtcgcccga gatttaattg
      361 agacaatggc gggtgccaag gggctcggat ataccagttt ggcgcaactt ccatgggcct
      421 aacactctgg cgccagagtt ccgagtaatc gtgaactgaa acttcttcta tgcaaaagcg
      481 cggattatag gcagctaaga tcggcgtcac ggctcgttcc gctcgttcgg catcacggcg
      541 gttttcgaaa taggctttaa actctcgtgg cggcgaaaga attcccggct cgagagccaa
      601 ctcaaattcg ttacccgcta acatcgattt gtcgtcggga ggagcggggg tcgcaccctc
      661 ttcaagacct tgagaatgga attgatagag aagaccgctg accaagtccc atgtgtcgtc
      721 gtctaaggtg cgttcgagtt tacaatccag tgaaatcatt ccgcggtgtc cggactgcgc
      781 cgggttaagc ctcttctttt ttctcggcta cgagcttgaa gattttaaac tccgccgaac
      841 cagcggtgta catgattttt cgcaaaatat cgaattttac gctcttatcg gcatgaataa
      901 tgacaacgcc ctgaaacttc ggggtagagc gctctccagt agtaggatct actaccacgg
      961 ctttgctcat catgagttcg gctttttggc gcgacttcac catggcgtca tagagaggta
     1021 gaattctgcg accgtagtcc gccaggtgac gggactcgat ttcataggtt ggggtgaacg
     1081 tgaatccagc aggggcaggg ctcggaatta cggggagatt tgcgaaatcc attacttttt
     1141 cgttatcgaa catgatgccg gtgggctcga ctacgagatt aatactatcg gctggaattt
     1201 cttcagcggt ggaagaaggc aatttaatgt tcgatgacgt ggcgaaagcg actgagttgg
     1261 tggcgtaact ttttagcaag aaaacaacca tgatgatcaa catgtccatc atcgacgtta
     1321 attgtaagtc aaagtctcgt ttgatgcggt gacgtcgacg gcggtttttt atggatgatc
     1381 tcacgtaagg ctccttcctg gctcgcgtta cgacataatg ttgccgaaaa taacctctga
     1441 aaagagtttg gtttcgggtt catcgattcc ctggtcgttt ttcttgtaga gtacggagtc
     1501 agttttttcg aaccgtcgag cagcgtccat gagtgccacg agcacttcat agg
//
LOCUS       DAOMXO010000611         1550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_47716_length_1550_cov_4.950502, whole genome shotgun sequence.
ACCESSION   DAOMXO010000611 DAOMXO010000000
VERSION     DAOMXO010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47716_length_1550_cov_4.950502"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLH57_10860"
     CDS             complement(<1..689)
                     /locus_tag="PLH57_10860"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HRK03153.1"
                     /translation="MAKAGFWMGFWAPLRAAKMILKDPKLLSLAAIPLALNIGLYILL
                     FHWGSGQIDALNQSLVQWMATKTPAWLTTLASWFLSIAAWVSLVLLSVISFTFVGSIL
                     SAPFNDGMSKRVYVIKQREALERGSSLEALPGLPLGTSLRLEILRTGILLSGGLFALL
                     VGLIPLLQLPALIMGAILLSFEYFGYPFAQKEPRLGPVWGFVFRHPMVSLGFVSAPSG
                     IGRARMRRKVPP"
     gene            853..>1550
                     /locus_tag="PLH57_10865"
     CDS             853..>1550
                     /locus_tag="PLH57_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03154.1"
                     /translation="MKKRTGFYSTSGFSFFRCARKVRLLGAVAIAVAAAGCSSSVLEE
                     NKGSDQPGANPGQGVTVASCRGVDLSSELLGVRQLREVIACLNGNHTIDAYQRLLERL
                     SDRQLTPLVDLLNAHLMTNGRRFKDLRESFEHLDQHGVLDAMLDRASDLLGENGLVSA
                     LIKLSKPAVLKDDGKVDLLVLASLEQIAKDIRSGKGARANLDKGLSAVARISALDSWA
                     EISRGLSVPIELPRP"
BASE COUNT          390 a          381 c          413 g          366 t
ORIGIN      
        1 ggcgggactt ttcgacgcat tctggcgcgg ccaataccgc tgggcgccga cacaaaacca
       61 agactcacca ttgggtgtcg aaatacgaaa ccccaaacgg gtccaaggcg cggctctttt
      121 tgcgcgaacg gataaccgaa atattcaaac gacagaagta tcgcacccat gatgagggcc
      181 ggtagttgaa gcagggggat taaacccact agaagcgcaa aaagtccgcc ggagagtaaa
      241 ataccggttc ttagaatctc taatcttaaa gaagtaccga gcggcaaccc cggaagcgcc
      301 tcaagcgaac ttcctcgttc aagcgcttct ctttgtttga tgacgtagac gcgtttactc
      361 attccgtcgt tgaagggggc cgacaaaatg cttccaacaa atgtgaagct aattaccgag
      421 agcaaaacca aactcaccca ggcggcaatc gacaaaaacc aggacgccaa ggtggtgagc
      481 caggcgggag ttttggttgc catccattgg accaaagact gattgagtgc gtcgatttgg
      541 cccgaacccc agtgaaacaa caaaatatat agcccgatat tgagtgcaag aggtatggcc
      601 gcaaggctca agagttttgg gtcttttaaa atcatttttg cggcgcgcaa tggtgcccaa
      661 aaccccatcc aaaagccagc ttttgccatt ttaatcatat cgccaagtta tcgaattcat
      721 ttggtttggg caagctttca cctgcagtct gcggattggc catgggtggg tggcggtcgc
      781 ttgacgtcat tgctcaggta agctaaggtc gccgcctgcg tttagacggc tgtttggagt
      841 ggagatacag tattgaaaaa gcgaacgggt ttttattcga cctcgggatt ttcttttttc
      901 agatgcgcac gaaaagtgcg ccttttggga gcggtcgcca tcgcggtagc tgcggcgggc
      961 tgttcgtctt ctgttcttga agaaaacaaa ggcagtgacc agcccggcgc caaccccggc
     1021 caaggcgtga cggtagcgtc gtgccgaggc gtcgatttat cttccgaact actcggggtt
     1081 cgacagctac gtgaagttat tgcgtgcctc aatggtaatc acacgatcga cgcctaccaa
     1141 cggctcctcg aacgactttc ggaccggcag ctaacacctc tagtagacct tctcaatgcg
     1201 cacttaatga ccaacggtcg tcgcttcaaa gatcttcgag aaagttttga acatctcgat
     1261 cagcacggtg ttctcgatgc gatgctcgat cgagcttcag atttacttgg cgaaaacgga
     1321 ttggtttctg cgctcattaa actgagtaaa cccgctgttt tgaaagacga tggcaaagtt
     1381 gatcttctgg tgctggcgtc gctagagcaa atcgctaaag atatacgtag tggtaaaggg
     1441 gcgcgagcca accttgataa aggtttatcg gccgttgcgc gaatttctgc gctggactct
     1501 tgggctgaaa taagtcgcgg attgagtgtt ccgattgaac ttccgcggcc
//
LOCUS       DAOMXO010000612         1549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_47773_length_1549_cov_4.192771, whole genome shotgun sequence.
ACCESSION   DAOMXO010000612 DAOMXO010000000
VERSION     DAOMXO010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47773_length_1549_cov_4.192771"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLH57_10870"
     CDS             complement(<1..440)
                     /locus_tag="PLH57_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRK03155.1"
                     /translation="MKRVIAFFVNQQVFGDLLTVFVVLAGLVSMTLIQREVFPNVSFD
                     IITVNTIFPGAAPEEIEKLITNPLEQEIKEVDGIKKVISGSVEGRSGIVVTLDPDQTS
                     EQEGKTDIQDVIDRFDDLPIDAERPIVTAVQSKQSPIIEVSVSGA"
     gene            complement(443..>1549)
                     /locus_tag="PLH57_10875"
     CDS             complement(443..>1549)
                     /locus_tag="PLH57_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRK03156.1"
                     /translation="ESEGSLTLSQSLVKNSFGYGTRRLKSAGDFSRRAAELELQENIE
                     LWSIDLISSFYNAWLAQAGAIAARHNLERRERLMNITAIKERRGTAEEPDRLQVQSAL
                     LAAKVERDHREKILGDRWRSLVTNLKLPSNWTEIDATLIPLVLDEPSADALAVCGSSL
                     EATQGPSESAATERLNMQARAAELEAQRTNNLLLPDLKIYGTLASNGVDPKQESRAFT
                     EMRTGKHPAYQIGIQLQYSLGQHLERAEASINRANAERLAALASASTDQLKIDWLNAC
                     ADLRRLVDGAKLRETAYNNQLRRAALEEERFRIGRTTTIGVIQSGDDAALADIDYRTI
                     DIERRIASWHVFHLSNQLGKKLPAKAKQFLTKDL"
BASE COUNT          350 a          376 c          401 g          422 t
ORIGIN      
        1 gctcccgaca cagacacttc aatgatagga gactgtttag actgaacggc agtgacgatt
       61 gggcgttcgg catcgatcgg aagatcgtca aaccggtcaa tgacgtcttg aatatcggtt
      121 ttgccttcct gttcagacgt ctggtcaggg tcgagagtca ccacgatgcc gcttcggcct
      181 tcgactgaac ccgagattac ttttttaatt ccatcgactt ctttaatttc ctgttcgagg
      241 ggattagtga tgagtttttc gatctcctcc ggcgccgctc ctggaaaaat cgtattcaca
      301 gtgatgatgt caaaactaac gttcgggaac acttcgcgtt gtataagggt catagaaact
      361 aacccagcca acacgacaaa cacggtgagt aagtcaccga aaacttgttg atttacgaaa
      421 aacgcgatga cccgtttcat atttagagat ccttagtgag aaactgttta gctttcgctg
      481 ggagtttttt tccaagttga ttgcttagat ggaaaacatg ccaacttgca attcgacgct
      541 cgatatcaat tgtccggtaa tcaatatcgg ccaacgcggc gtcgtcaccc gattgaatga
      601 ccccgatcgt ggtggtgcgt ccaatgcgaa agcgctcctc ttctagagct gctcttcgaa
      661 gttggttgtt ataggcggtt tctcgaagtt tggctccgtc aactagacgt cgaagatcgg
      721 cacacgcgtt gagccagtca attttgagtt gatcggttga tgcgctggca agtgccgcta
      781 gtcgttcagc attagctcga ttgatcgatg cttcggcgcg ttccaaatgt tggcctaaac
      841 tatattgaag ttgaatgcca atctgatatg ccgggtgttt tccggtgcgc atttcagtaa
      901 atgcccgaga ctcctgcttg gggtcgactc cgttcgatgc aagcgtgccg tagatcttta
      961 aatcgggaag gagcaagttg ttcgttcgct gagcctcgag ttcggcggca cgggcttgca
     1021 tgttaagtcg ttctgtggcg gcgctttccg aaggcccttg agtcgcttct aatgaagacc
     1081 cgcacacggc aagtgcgtcg gccgacggct cgtccaacac cagtggtatc aatgtagcgt
     1141 cgatctccgt ccagttggaa ggcaatttga gattagtgac cagagatctc catcgatcac
     1201 caagaatttt ttcgcgatgg tctcgttcga ccttggcagc aagtaacgcg ctttgaactt
     1261 gaagtcgatc gggctcttct gcggtacccc ggcgctcttt tatagcggta atgttcatga
     1321 gccgctctcg tctttcaagg ttgtgtcgcg ccgcaatagc gccggcttgc gcgagccagg
     1381 cattgtaaaa tgaagaaatt aaatcaatcg accagagttc gatgttttct tgaagctcaa
     1441 gctccgccgc ccggcggctg aagtctccag cggacttaag gcgtcgtgtg ccgtagccga
     1501 agctgttttt aactaaactt tgagaaagag taagtgaccc ttcgctttc
//
LOCUS       DAOMXO010000613         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_47956_length_1545_cov_3.666443, whole genome shotgun sequence.
ACCESSION   DAOMXO010000613 DAOMXO010000000
VERSION     DAOMXO010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47956_length_1545_cov_3.666443"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..>1545)
                     /locus_tag="PLH57_10880"
     CDS             complement(100..>1545)
                     /locus_tag="PLH57_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK03157.1"
                     /translation="IALKKGVFFHDDPCFAGGKGREFNVDDVIFMIERICDPKTRSEM
                     YGAFEGRLLGAKEFFVGKTKSIQGIRRIDNYTLELTLEKPLPRFVSVFADNKTSIVPR
                     ECVKKYGDDFRNRAVGTGPFLLQEANLASKVVAVRNPNYNHMTYPSEGNPGDAEKGLL
                     TDAGKKLPLVDKVVLEIITESQPGWLKFLAGDFDIGRIPKDSVHSVINGESLSPEYAA
                     KRIELSRQMKGDVTMLIFNMEDQLWGKQKELRQAVALALNVPEVIEMQYSKQAIRAHS
                     LIDPTQYGYESGFQSKWANQNVALAKELLAKAGYPDAKGLPPIHFLNGTDTASRHLEG
                     LLSKQFKAVGITLKPEGMTFPEMLRRVKDTGNFTFYGQAMVSTVSDADDSLASYHSKN
                     IKSGYNPSRYNNPIVDQLIDEIEFMENGPERLAKIKKVQEIVDEDLPIIPMVHRIANQ
                     PSHYWLKNKVHIDETFYAFLKYLKIETANGP"
BASE COUNT          388 a          351 c          319 g          487 t
ORIGIN      
        1 ctgctagatc gaacggggca agggtcctca gtcgttccaa taaatctgaa actcgaacca
       61 cattagattt atttttcttt ttcatttttt agccaccggt tacggaccgt ttgctgtctc
      121 gatttttaga tactttaaga aagcgtaaaa agtttcgtcg atatgaactt tatttttgag
      181 ccagtaatga ctgggttgat tagctattcg atgaaccatt ggtataatcg gtaagtcttc
      241 gtctacgatt tcttgaacct tcttaatctt tgccaaacgc tctggcccat tctccatgaa
      301 ttcgatttca tcgatcaatt ggtcgacaat cggattatta tagcgcgagg gattgtaacc
      361 ggacttgatg ttttttgaat ggtaagaagc aagcgagtcg tctgcgtccg agactgtaga
      421 aaccatggct tgtccataga atgtaaaatt cccggtatct ttcactcttc gcaacatttc
      481 aggaaaagtc attccctcgg gcttcaaagt gatacctact gctttgaact gttttgaaag
      541 taatccctct aggtgcctcg acgcagtgtc ggtaccgtta agaaagtgaa ttggtggtag
      601 ccccttggcg tcgggataac ccgccttcgc aagtagctct ttggctagtg ctacattttg
      661 attggcccat ttactctgaa atccactctc gtaaccgtat tgagtcgggt caattagtga
      721 atgagcgcga atggcttgtt tcgaatattg catttcgata acttcgggca cgttcaacgc
      781 aagtgcgaca gcttggcgca attctttttg tttgccccag agttgatctt ccatgttgaa
      841 gatcaacatc gtgacatcac ccttcatttg tcgagaaagt tctattcttt tggcagcata
      901 ttccggcgac aatgattcac cattaattac agagtgcacg ctatcttttg gaattcgacc
      961 aatatcaaag tcacctgcca aaaatttcaa ccacccaggt tgcgattcag taatgatttc
     1021 gagaaccact ttatctacta gtggaagctt ttttccggca tcggttaaaa ggcccttttc
     1081 agcgtctccc ggattacctt cactcggata agtcatgtgg ttgtaattcg gatttcgaac
     1141 tgcaaccacc ttcgaagcaa gattagcttc ctgtagcagg aaaggtccgg ttccgactgc
     1201 gcgatttcta aaatcgtcgc catatttttt tacgcactca cgtggaacta ttgaggtttt
     1261 attgtcggcg aacactgaca caaatcttgg cagcggtttt tcaagtgtga gttccaacgt
     1321 gtagttatct attcggcgaa tcccctgaat agacttggtt ttacctacaa aaaattcctt
     1381 tgcgccaagt aatcgccctt cgaatgcccc atacatttcg gaacgtgttt tgggatcgca
     1441 aattctttcg atcataaata tcacatcgtc gacgttaaat tctcgtccct tgcctccagc
     1501 gaaacacgga tcatcgtgaa agaagactcc ttttttcaga gctat
//
LOCUS       DAOMXO010000614         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_47962_length_1545_cov_3.430872, whole genome shotgun sequence.
ACCESSION   DAOMXO010000614 DAOMXO010000000
VERSION     DAOMXO010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47962_length_1545_cov_3.430872"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLH57_10885"
     CDS             <1..471
                     /locus_tag="PLH57_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03158.1"
                     /translation="FGELPVATLADEIEGDDDSEARALAWGELVRRETRGALVRNDSL
                     LHASARDSVRDRSARDHSVRDHSVRDHSVRAPSPAPSARNAEVVELPLDRPVAMIRPR
                     CTAGQVVAPIELADGTYVASGSTLTESVEAKDSFVIARERQVTKPGYAKKYKRK"
     gene            504..>1545
                     /gene="metG"
                     /locus_tag="PLH57_10890"
     CDS             504..>1545
                     /gene="metG"
                     /locus_tag="PLH57_10890"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRK03159.1"
                     /translation="MSKKNFYITTAIDYPNGAPHMGHAYEKIITDAYARWARSSGLET
                     YFLTGTDENGQKLAKSAEELNVPTRKFVDDNAEIFKKLCSELKISNDDFIRTTESRHS
                     KVVGEFWRKLEKNGDVYFDRYSGLYCLACETFYTETQASDRLCPEHKTKLELLEEEGY
                     FFKMSKYEKWIKEFLTGNPAFVAPSEARNEIVSRLNSEPLKDLSISRPNKGWGIPVPG
                     NDKFVVYTWFDALINYYSAVVGTSHENYWPADLHVIGKDIGWFHTVIWPIMLHALGIP
                     TPAQVYIHGMVLSEDGRKMSKSLGNGVDPFDVLKTYPLDSFRYFMLRSIPSGGNGRFS
                     MKDLNQRHVSELG"
BASE COUNT          408 a          375 c          396 g          366 t
ORIGIN      
        1 ttcggcgagc tgccggtggc gacgctcgcc gacgagatcg agggcgacga cgactccgag
       61 gcgcgtgcgc tcgcgtgggg cgagctcgta cgacgagaga cgcgtggtgc gctcgtgcga
      121 aacgactcgc ttcttcacgc ttcggctcgc gactccgtgc gcgatcgctc cgcgcgcgat
      181 cactccgtgc gcgatcactc cgtgcgcgat cactccgtgc gagcgccctc ccccgcgccg
      241 tcggcacgca acgcagaggt ggtggagctg ccgctcgatc gaccggtcgc catgattcgg
      301 ccgcgctgca cggcaggtca ggtcgtggcg ccgatcgaat tggccgacgg aacctacgtg
      361 gcgtctggct ctactctaac cgagtcggtt gaagccaaag acagttttgt gatagctcga
      421 gaaaggcaag tcactaaacc agggtacgca aaaaaatata agagaaaata agtactccta
      481 aaaaacctaa agaagcgaca aaaatgtcaa aaaaaaattt ttatatcact actgcaattg
      541 attatcccaa cggggcgcct catatgggtc acgcctatga aaaaatcatt accgatgcat
      601 atgctcgttg ggcccgctcg agtgggctcg aaacttattt tttaacgggc actgacgaaa
      661 atggccagaa actcgccaaa agtgccgaag aattaaatgt tccgacacgg aaatttgtcg
      721 acgacaatgc cgaaattttt aagaagttgt gttctgaatt gaaaatatca aacgatgatt
      781 tcattcgtac aaccgaatcg cgtcactcaa aagttgtagg tgagttttgg cgtaaactcg
      841 agaaaaacgg cgacgtatat tttgatcgct acagcggact ttactgttta gcgtgtgaga
      901 ccttctacac tgaaactcaa gccagcgacc gcctctgccc cgaacataaa acaaaactcg
      961 agctacttga agaagaaggg tatttcttca agatgagtaa gtatgaaaag tggattaagg
     1021 aatttttaac tggaaacccc gcgtttgtgg cgccttccga ggctcgaaat gaaattgttt
     1081 cgcgtctaaa tagcgaacct ttaaaagatc tttccatttc gcgccctaat aaaggttggg
     1141 gtattcccgt gcccggtaac gacaagttcg ttgtgtatac ctggtttgac gctctaatta
     1201 attactacag tgcggtagtg gggacttccc atgaaaacta ttggccggcc gatttgcatg
     1261 taatcggaaa agatattggg tggtttcata ccgttatttg gcccatcatg ctgcatgccc
     1321 tgggaatacc gacacccgcg caagtgtaca tacatggcat ggtgctttct gaagacgggc
     1381 gaaaaatgtc taagtcactc ggcaacggag tcgatccgtt tgatgtgctt aaaacctatc
     1441 cgctggatag ttttagatac tttatgttgc gatctattcc gagcggcgga aatggccgct
     1501 tttcaatgaa agatctcaat caaaggcacg tttcagaact cggta
//
LOCUS       DAOMXO010000615         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_47972_length_1545_cov_2.462416, whole genome shotgun sequence.
ACCESSION   DAOMXO010000615 DAOMXO010000000
VERSION     DAOMXO010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47972_length_1545_cov_2.462416"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PLH57_10895"
     CDS             <1..984
                     /locus_tag="PLH57_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK03160.1"
                     /translation="KMLSSEDVLPKEREMFLGMALRNAERLNSTLNELLDLSKLVSGR
                     LVCRFQEVPLKNLLLPILDKFCDRANAAGYELTLSIERDLPVILGDAFRLEQVFRSIC
                     ENALKFGSPGGKLNVKLAVERVGKVVFECTNPTTSNITAKELRDIFTIFSQREAVLDR
                     VHEGVGGSLAIAAETIAQHEGVLNAELANGQFAVRVELPVLDNERALLKVLESRIYAL
                     RTELGATSLLILEVPVKSFQAVVSTLRSALFRASDAVYTLPRAGQIAVVMDDCKKSDA
                     PKIVSRLFAEMNREAAKFLTNARAGLASFPEDSTDPEELLVLSRQRLEKVK"
     gene            1086..>1545
                     /locus_tag="PLH57_10900"
     CDS             1086..>1545
                     /locus_tag="PLH57_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRK03161.1"
                     /translation="MSADLNFSPDAKKTFYIKTFGCQMNVADTERMSVLLAESGAEQA
                     ATAEQADVIILNGCEVREKAVHKMVSALGGMTKNRGRVDRAQGKKQIIGIGGCVGQLD
                     GAKLFKRNPHIDFVFGTDTIDQLPEIVHEVQSGHRHVVYNEFDKTREYSTD"
BASE COUNT          383 a          368 c          434 g          360 t
ORIGIN      
        1 aaaatgctta gcagtgaaga cgtgttgcca aaagaacggg aaatgttcct tggaatggcg
       61 ctacgaaatg ccgaacgttt gaattcaacc ttaaacgagc tgctggattt atcgaaactt
      121 gtatctgggc gattggtgtg tcgctttcag gaagtgccat taaaaaatct tctgctgccg
      181 attctcgaca aattttgcga ccgtgcgaac gctgcgggtt atgagctgac gctctcgatc
      241 gagcgcgatt taccggtgat actcggcgac gcgtttcgat tggaacaagt gtttcgatcg
      301 atctgcgaaa atgcgctgaa atttggttcg cccggtggca aactgaacgt aaagctagcc
      361 gtggaacgag tcggaaaggt ggtgttcgaa tgcaccaatc ccacgacatc gaatataact
      421 gctaaggagc tgcgggatat ttttacaata ttttcgcagc gagaggcggt gctcgatcgg
      481 gtccacgaag gggtaggcgg atctctggcg attgcggccg aaaccattgc gcaacatgag
      541 ggcgtattga acgccgaact agcgaatggt cagttcgccg ttcgagtaga gcttccggtg
      601 ctcgacaatg agcgggctct gctaaaggtt ttagagagtc gcatttacgc actacgcacc
      661 gaacttggcg ccacatcgct tttgatacta gaggtcccgg taaagtcatt tcaagcggta
      721 gtgtccacac tgcggtctgc gctttttcgg gcatctgatg cggtgtacac gctcccgcgg
      781 gcagggcaga ttgccgtcgt catggacgat tgtaaaaaat ccgacgcccc gaaaattgtt
      841 tcgcgattgt ttgccgaaat gaatcgcgaa gctgcaaagt ttttaaccaa cgcccgggcc
      901 gggttggcta gtttccccga ggactccacc gacccggaag agctcttggt gctctcgcgg
      961 cagcggttgg agaaggtaaa atagggtttt tgttgcccgc cccccggtcg cttgcgcccg
     1021 cctcgcggcc agaccatgga agttattgac tcgattagtc agaacgtata agtcaggctc
     1081 tactcatgag tgccgactta aacttttcgc cagatgcgaa aaaaactttt tatatcaaga
     1141 cgtttggttg ccaaatgaat gtggcagaca ccgaacgcat gtcggtgctt ttggcggaat
     1201 cgggcgcaga gcaagcggcg acggcggaac aggccgatgt catcatccta aatggctgcg
     1261 aagtgcgcga aaaagcagtt cataaaatgg tttcggcctt gggaggaatg accaaaaacc
     1321 gtggtcgagt ggaccgggcc cagggcaaaa aacaaattat tggaatcgga ggctgcgtcg
     1381 gtcagctcga cggcgctaag ctttttaaac gtaatcccca cattgatttc gttttcggaa
     1441 ccgacacgat cgatcagttg cccgaaattg tgcacgaagt gcaaagcgga catcgccacg
     1501 tggtttacaa cgagttcgac aaaactcgcg aatattcgac cgaca
//
LOCUS       DAOMXO010000616         1544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48006_length_1544_cov_3.927468, whole genome shotgun sequence.
ACCESSION   DAOMXO010000616 DAOMXO010000000
VERSION     DAOMXO010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48006_length_1544_cov_3.927468"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..946
                     /locus_tag="PLH57_10905"
     CDS             140..946
                     /locus_tag="PLH57_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRK03162.1"
                     /translation="MTVQKMPNTKTIRLENKGGVLTIELSRPDVRNAFNDELITDLQN
                     TFDWLANDPSVRVTVLKGSGPVFCAGGDLNWMQRSVQLSRDENLADTRKLSKMFYTMN
                     LSPKPLVGLVQGAAIGGGVGLVSVCDYVVAEKKTTFSLSEVRLGIVPACIGPFVIAKI
                     GESYARAYFISAERFDAGRAREIGLVHELVDSAAELQIAQDRITQNLLQCGPNAMAVA
                     KSLVRSIKECEPGKVMEHVSCLLADLRVSPEGQEGLKAFLEKRKPNWVRS"
     gene            943..>1544
                     /locus_tag="PLH57_10910"
     CDS             943..>1544
                     /locus_tag="PLH57_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRK03163.1"
                     /translation="MKKTEKKSKTETAIKRLMVANRGEIARRVFASAKNLGIETLAVY
                     SEADSEMSFVKEADRSVMIGPGPAAESYLSIKKILEVAKSEKVDAIHPGYGFLSERAE
                     FAKAVIDAGIQWLGPSPRVIELMGNKVAAKKAAESAGVPTLPWAILAEGWTAATLKAS
                     AKKVGFPLLLKAAAGGGGRGMRLVHGEGELLEKAESAAREA"
BASE COUNT          413 a          321 c          435 g          375 t
ORIGIN      
        1 gactctggga cgacggaatt atttcgccag tagacacgcg caaggtatta gcattgggtc
       61 tggcggcagc ctataacgca ggattaccga aaacggattt tggcgttttt cgaatgtaag
      121 aaaacgaagg ggtttgttta tgacggtaca gaagatgcca aacaccaaga cgattcgttt
      181 ggaaaataag ggtggagtgt tgacgataga gttgtcgcgc cccgatgtac gcaatgcttt
      241 taacgacgaa cttataactg atttgcaaaa cacctttgat tggctcgcaa acgaccccag
      301 tgtgcgagtt acagtgctca aaggttccgg tcctgttttt tgtgcgggtg gtgatttaaa
      361 ttggatgcaa cgaagcgttc agttgtctcg cgacgaaaac ctcgccgata cccgtaaatt
      421 atcgaaaatg ttttatacca tgaacctttc acctaagccg cttgttggct tggtacaggg
      481 cgctgccatt ggtggtggcg taggccttgt tagtgtttgc gactacgtcg ttgcggaaaa
      541 aaagactact tttagtctga gcgaagttcg tttgggaatt gtaccagctt gcattggtcc
      601 ttttgtgata gccaaaatag gtgaatcata tgcgcgggct tattttattt cggcggaacg
      661 gttcgacgcg gggcgcgcgc gagaaatagg cttggttcac gaacttgttg attcagcagc
      721 tgaactacaa atcgctcagg atcgcatcac ccaaaatctt ttgcagtgcg gccctaacgc
      781 aatggcggta gctaagtctc tggttcgcag cataaaagaa tgtgaaccgg gaaaagtaat
      841 ggaacacgtt tcatgtctgt tggccgatct gcgagtgtct cccgaaggcc aagaaggatt
      901 aaaggcgttt ttggaaaagc gaaaaccgaa ttgggttcgt tcgtgaaaaa aactgaaaaa
      961 aaatcaaaaa ccgaaactgc tattaagcgt ctcatggtcg cgaaccgagg tgaaatcgcg
     1021 agacgggttt ttgcgagcgc aaaaaattta ggaattgaaa cattagcagt atattctgaa
     1081 gcagatagcg aaatgagttt cgtgaaagaa gctgatcgct cagtgatgat tgggccaggt
     1141 cccgctgcgg agagctatct atcaataaaa aaaattctcg aagtcgcgaa aagtgaaaaa
     1201 gtcgacgcca ttcacccagg gtatggcttt ctttctgagc gagccgaatt tgccaaagcg
     1261 gtcatcgacg ccggcattca atggttgggg cctagcccgc gagtcattga actcatggga
     1321 aacaaggtgg ccgccaagaa agcggctgaa agcgcgggag ttcccacgct gccttgggcg
     1381 atcttagcag aaggctggac cgcggcgaca ctgaaggcat ctgcaaaaaa agtcggattt
     1441 ccacttttat tgaaagccgc tgctggtggt ggtgggcgcg gcatgcgctt ggtgcacggt
     1501 gagggtgagc ttttggaaaa ggccgaatcc gcggcgcgtg aagc
//
LOCUS       DAOMXO010000617         1543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48048_length_1543_cov_4.099462, whole genome shotgun sequence.
ACCESSION   DAOMXO010000617 DAOMXO010000000
VERSION     DAOMXO010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48048_length_1543_cov_4.099462"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLH57_10915"
     CDS             <1..513
                     /locus_tag="PLH57_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03164.1"
                     /translation="TWRTVGPYSLEEIPEDYEWLHRFFLMGFKAEKIPANLILWRDHS
                     TRLTRTHAAYADDKFVEVKRRFVNQRLKTTGKTRLWFFGLGPTGKRIFTMVMGFGWPI
                     GGVVDVSPKRIGMAWNGFEIQSPVSWSRQWRSESDLVLIGVGTAEQRAACESFCRENG
                     LSESEYLALS"
     gene            complement(517..744)
                     /locus_tag="PLH57_10920"
     CDS             complement(517..744)
                     /locus_tag="PLH57_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03165.1"
                     /translation="MQRNPENDSTSLTFEEYRDCFGDAGEVMKRHLLCTLCGGHLHFN
                     HMADFRNGIIQETARCPDCGIRVRARLHKLQ"
     gene            complement(864..>1543)
                     /locus_tag="PLH57_10925"
     CDS             complement(864..>1543)
                     /locus_tag="PLH57_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03166.1"
                     /translation="KKLTDAYIEALAPYTTNKTTTCTSNSIPVFGTKLGSFLNNTITS
                     DYFWVNHCTKSTLTSPNYSGLSSYFELRKNGNFFLNMDYNRGEDFLLSNGSKVRTYKY
                     WRAPIYEKTNTGQLPKARNTIFAPGHSIIYDSVYESNGLRYSFRTALPMNIEETQEFL
                     TDKRKFEDWILRVEVGFNQFLNTNSATIAPKECAAAQAPVSSALPATNTSISNSENEP
                     SEGLEAD"
BASE COUNT          391 a          315 c          405 g          432 t
ORIGIN      
        1 acatggcgca cagttgggcc gtattcttta gaagaaatcc cagaagacta tgagtggtta
       61 catcgtttct ttttaatggg atttaaggcc gaaaaaattc ctgcaaattt gattttgtgg
      121 cgagaccatt ctacgcgact gacccggacc cacgccgcgt atgctgacga taagttcgtc
      181 gaagttaaac gccggtttgt gaaccagagg cttaaaacaa cgggcaaaac tcgcctatgg
      241 ttctttggtt tgggccccac aggaaagcga atctttacaa tggtgatggg tttcgggtgg
      301 cctattggtg gagtagtcga cgtgagtccc aaaagaattg gaatggcctg gaatggtttt
      361 gaaattcaaa gcccggtaag ttggagtcgt cagtggcgga gtgaaagtga tttagtacta
      421 attggcgtag ggaccgcgga acaacgcgct gcctgcgaaa gtttctgtcg cgaaaacggg
      481 ttgtctgaga gtgaatattt agccctgtcc tgacggttat tggagtttgt gcaagcgcgc
      541 gcgtacgcga atgccgcagt caggacagcg agcggtttct tgaatgatac cgttgcgaaa
      601 gtcagccatg tgattgaaat gtaggtggcc accgcaaagc gtgcataaca aatgccgctt
      661 catgacttcg cccgcgtcac caaaacaatc gcggtattct tcgaaagtga gggacgttga
      721 atcgttctct ggatttcttt gcatgacacg cgcctccgtg cggttaaagc gtcactgaat
      781 acaaagactc ttcggcgcag tcgcgtgatt catttagaga gtggagtggg taagatattt
      841 agggctcgat ggaggcagag cgactagtct gcctcaagac cttctgacgg ttcattctcg
      901 gaattagaaa tcgatgtatt cgtcgcgggt agcgccgacg aaaccggggc ttgcgctgcc
      961 gcacattctt tgggggcaat tgttgccgag ttcgtattta aaaactgatt aaaacctact
     1021 tccacacgca ggatccaatc ttcaaacttt cgtttgtcag tgagaaattc ctgggtttct
     1081 tcgatgttca ttgggagtgc ggttctaaac gagtatctca atccgttcga ttcatagacg
     1141 gaatcataaa ttatgctgtg ccccggcgcg aaaatggtat tgcgcgcttt tggtaattga
     1201 cccgtatttg ttttttcgta aatcggggct ctccagtatt tataggttcg aactttcgag
     1261 ccgtttgaaa gtagaaaatc ttcgccacga ttatagtcca tgtttaagaa aaaattcccg
     1321 ttcttacgta gttcgaagta actcgataaa ccggaatagt ttggtgaagt taaagtgctc
     1381 ttggtgcaat gattaaccca aaagtagtca ctagttattg tattgttcaa aaaactgccc
     1441 aatttagtac cgaacaccgg aatcgaatta gaagtacagg tagtggtttt gttcgtagtg
     1501 tagggcgcca gcgcttcaat gtaggcgtcg gttaattttt ttt
//
LOCUS       DAOMXO010000618         1543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48059_length_1543_cov_2.862231, whole genome shotgun sequence.
ACCESSION   DAOMXO010000618 DAOMXO010000000
VERSION     DAOMXO010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48059_length_1543_cov_2.862231"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLH57_10930"
     CDS             complement(<1..596)
                     /locus_tag="PLH57_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRK03167.1"
                     /translation="MSKRVWNEEQTALIELDSAGRPRLNQVAVRADAGAGKTTVLIER
                     VRRLLEQPHAARLVCVSFTEKSKADLAERLRDQPRAEIYTIHGFCKRVIDEFGERAGW
                     LPYPRVIDENVADAVFDKAFERVARNLSKSDPLFQSGALAALGFVNLKLFSQLMILRS
                     DPIRGAELRGSGFTEAALAALRSFSSLVHREFDLAKNK"
     gene            complement(593..>1543)
                     /locus_tag="PLH57_10935"
     CDS             complement(593..>1543)
                     /locus_tag="PLH57_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRK03168.1"
                     /translation="EAYECVDRERAAGFLESGKIRVTAFEDYVKCPRLFYERHVRKNE
                     PLQALSLEVDPRVRGDSLHSVLETLTKKKMRAQVEFASLTNATIKDLVAHTVREKTKN
                     RTRSRQFAHPSLALPLIDELTDRVSAWVRREVEMETITPALTPKLVEHRFELLLDERS
                     PIRLIGTMDRLDTAGSHAVIIDYKTGVPRFSGKDLKAGYGVQLVAYASAVEEQGLVPV
                     ASLYLVVQRFESKVAGGLLCKPYNKKYFRVGPTSSGLVEADDLSETLKNARRKWPEYL
                     EALQNGNFTADPLDPAVECRTCAYRLICGFKEKTNSGTQS"
BASE COUNT          380 a          412 c          362 g          389 t
ORIGIN      
        1 attttatttt ttgcgagatc aaattctcga tgcacaagtg aagaaaagct cctaagtgcc
       61 gcaagcgctg cctcagtaaa accactaccg cgcaattccg cgccacgtat gggatcggaa
      121 cgcaaaatca ttaactgcga aaataattta agattcacga accccagggc tgccagcgca
      181 ccagactgaa ataacggatc acttttggat aagttacgag ccactctttc gaaggcttta
      241 tcaaacactg cgtctgctac gttttcgtca attacgcgag ggtacggaag ccaccctgcc
      301 cgttcaccga actcgtcgat cactcgttta caaaaaccgt gaatcgtata aatttccgct
      361 ctcggctgat cgcgtaatcg ttctgcaaga tctgctttag atttttcggt aaacgacacg
      421 cacaccaacc gtgccgcatg aggttgttcc aataaacgcc gcactctctc gattaatacg
      481 gttgtcttac cggcgccagc gtcggcacgg accgcaactt gattcaaccg cggacgtcct
      541 gctgaatcta gttcgataag cgcagtttgt tcttcgttcc aaacgcgttt actcatgatt
      601 gagttcccga attagttttt tctttaaaac cgcaaatcaa ccgatatgcg caggtgcgac
      661 attcgaccgc agggtcgaga ggatcggcgg tgaaattgcc gttctgtaga gcctcgagat
      721 attcgggcca ctttcttcgc gcatttttca aagtctcgct caagtcatcg gcttcgacca
      781 aaccggaact ggtgggcccg acgcgaaaat attttttgtt gtagggttta cacaataaac
      841 cacccgcgac ctttgactca aaccgctgca ccacaagata caaggacgcc actggcacca
      901 ggccttgttc ttcgaccgct gatgcgtagg cgaccaactg cacgccatag cccgctttga
      961 gatctttacc cgagaaacgt gggacccctg ttttatagtc gataatcacg gcatgtgaac
     1021 ctgccgtgtc caaacgatcc atcgtaccga tcagtctaat cggcgagcgc tcgtctagca
     1081 gaagttcaaa gcgatgttct acgagtttcg gggtgagcgc tggggttatt gtttccattt
     1141 ctacttcgcg gcgaacccaa gcactgactc gatccgtaag ttcgtcaata agtggtaacg
     1201 cgagtgaggg gtgggcaaat tgccgggatc tcgttcggtt tttagttttt tcgcgcaccg
     1261 tatgcgcgac caagtccttg atggtggcat tggtcaaact agcgaactct acttgagcac
     1321 gcattttttt cttagtaagc gtttccagca ccgaatgcag cgaatcgccg cgcacgcgcg
     1381 ggtcgacttc gagcgaaaga gcctgcagag gttcgttttt tcgcacatgg cgttcgtaaa
     1441 acagacgagg acatttaacg taatcttcga aagcagtgac gcgaatctta ccagactcaa
     1501 gaaagccagc cgctcgttcc cgatctacac attcataggc ttc
//
LOCUS       DAOMXO010000619         1542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48081_length_1542_cov_4.427707, whole genome shotgun sequence.
ACCESSION   DAOMXO010000619 DAOMXO010000000
VERSION     DAOMXO010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48081_length_1542_cov_4.427707"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="PLH57_10940"
     CDS             complement(<1..1526)
                     /locus_tag="PLH57_10940"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRK03169.1"
                     /translation="MFLINFDDKGSVRRFWSLSIRAKIILLVTLTLTVSLCSYLYIGT
                     TLIIGDKVSYIYDYNLSQSRISTQSFDSWISKIVSNTRMMGAFLRSDGQYDREFGETL
                     FQRFDKSIGVESALFFRAQDSAFVSYAAFGQEQTVLEGYLVAMGWTPSQFNETEMLLG
                     TNPDGKLLIASHGVDLTGAGVYLLAVAKPDAALLKGVGSKDTSIFLYDSAGKRLTSSG
                     SDQALGASGELSEKAMTSIVKELLASTFPSGVREWVGPKKNDYILSYERLGKSKFTVV
                     ASIPKATAFAAAHALVQRSFALGVSILLVSIGLAMLFVKGLTRRIRELWNATRQVSEG
                     DFSVRVQTESNTADEINSLGQSFNTMADKIHELMAQTAEKARMEKELETAQVVQSRFF
                     PVQHFEHMNLTLAGRVVPASECGGDWWFYAPIGNRLLVVIGDVTGHGVSSALVTAAVH
                     GGFTMLIQKFMRDQAKVSIKEVIETLNAVVFAAAKQEATMTFVASLVDLDSGEMEFVN
                     A"
BASE COUNT          430 a          406 c          335 g          371 t
ORIGIN      
        1 gcgtttacaa actccatttc accactgtcg agatcaacca aactcgcgac aaaagtcatg
       61 gtcgcttctt gtttagctgc tgcaaaaacc actgcattga gtgtttcgat tacttcctta
      121 atagagacct tggcctgatc gcgcatgaac ttttgaatga gcatcgtgaa gccgccgtgc
      181 acagctgccg tcacgagcgc ggaagacacg ccgtgcccgg tcacgtcacc gattacgact
      241 aggagtcggt ttcctattgg agcatagaac caccagtcac caccgcactc actagcgggc
      301 acaacgcgac cggcaagcgt aaggttcatg tgttcaaagt gctgcactgg aaaaaatcga
      361 ctttgcacca cttgcgcggt ttcaagttcc ttctccattc gtgctttttc ggcagtctga
      421 gccatgagct catgaatctt gtccgccata gtattaaacg actgccctag actattgatt
      481 tcgtcagcag tgttactctc ggtttgcacg cgcaccgaga aatccccttc cgacacctgg
      541 cgcgtagcat tccacaactc acgaattctt cgagtgagcc ctttaacgaa caacatcgca
      601 agaccgatcg acaccaataa aatgctgacg ccaagagcaa acgatctttg tacgagagca
      661 tgagccgccg caaacgcagt ggctttaggt attgacgcga cgaccgtgaa tttgctcttc
      721 cctaggcgtt cataacttag aatgtaatcg tttttcttag gcccgaccca ttcgcggacg
      781 ccagaaggaa aagtagaagc aagtagttct tttacaatgg acgtcatagc tttttcactg
      841 agctcaccac tagctccaag cgcctgatca gaacccgaag aagtcaatct cttgcctgca
      901 ctgtcgtata aaaagatact ggtatccttg ctgcccacgc ccttgagcag agccgcgtct
      961 ggtttagcaa cagccagtag gtaaactcct gctcccgtta aatcaactcc gtgggacgcg
     1021 attagcagtt taccatctgg gttcgtaccc aaaagcattt cagtttcgtt aaactgtgaa
     1081 ggagtccaac ccatggccac taaataacct tcgagcacgg tctgttcttg gccaaatgcc
     1141 gcgtaggaca caaaggccga atcctgagca cgaaaaaaca acgcactttc tacgccgata
     1201 gatttatcaa atcgttgaaa caatgtttca ccgaactctc gatcatactg cccgtcgctc
     1261 cgcagaaatg cgcccatcat ccgggtgttt gaaacaattt ttgaaatcca agaatcgaaa
     1321 ctctgagtag aaatacgaga ctgagagaga ttgtagtcgt aaatgtaaga cactttatca
     1381 ccaataatca gagtcgtacc aatataaagg taggaacaca gactcaccgt cagcgtaagt
     1441 gtaacgagaa gaatgatttt agctctaatc gagagcgacc agaagcgtct cacgctacct
     1501 ttatcgtcga aattaatgag aaacatgaga tttccgataa gg
//
LOCUS       DAOMXO010000620         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48167_length_1540_cov_4.593266, whole genome shotgun sequence.
ACCESSION   DAOMXO010000620 DAOMXO010000000
VERSION     DAOMXO010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48167_length_1540_cov_4.593266"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /gene="lysS"
                     /locus_tag="PLH57_10945"
     CDS             complement(<1..1102)
                     /gene="lysS"
                     /locus_tag="PLH57_10945"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRK03170.1"
                     /translation="MKKPNISDWNEQIEVRFEKANRMIEKGINPFKNGLTPDHTAAQI
                     HQKYGELPKENLESVAPRASVAGRIMAVRDFGKGGFLRLQDRSGQIQIFLSKDVLSAD
                     VFDRYKEFTDLGDIVYIEGTVFRTKTNELSVRAERFEFLTKALRPLPEKFHGISDVEL
                     RYRHRSLDLVMNEAARKTFSARTKIVAAIRRFLDERDFLEVETPMMHPIAGGAAARPF
                     KTHHNALDMTLFMRIAPELYLKRLVVGGFERVYEINRNFRNEGISIKHNPEFTMLEFY
                     WAYATYEELMTMTEEMIQYVAQNVLGRTEIEYQGTKIDLKAPWQRLPVKEGVAKYSGF
                     KDLNKMNDRKALMTYLDSKKIAFDPKWTTGALL"
     gene            complement(1099..>1540)
                     /locus_tag="PLH57_10950"
     CDS             complement(1099..>1540)
                     /locus_tag="PLH57_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK03171.1"
                     /translation="LTRFASKVYMVHRRDQLRASKIMADRVAKNPKIQMIWDSAVEEV
                     LGDADVTGVRLKNLKTGAVTDLKVTGLFIAIGHAPNTRIFNKQLSLDANGYIVTQNGT
                     KTNLPGVFAAGDVQDHVYRQAVTAAGTGCMAALDAERYLEGLHE"
BASE COUNT          396 a          384 c          324 g          436 t
ORIGIN      
        1 ttaataaagc cccggtggtc catttcggat cgaatgcgat ttttttagaa tcaagataag
       61 tcatcaaggc cttacgatca ttcattttat tgaggtcttt aaatccagaa tacttcgcca
      121 caccttcttt tactggcagc ctctgccacg gagccttgag atcaattttc gttccttggt
      181 attcaatctc ggttcgcccc aatacatttt gtgcgacata ctggatcatt tcttcagtca
      241 tggtcatgag ttcttcataa gtcgcatagg cccagtagaa ttccagcatg gtgaattccg
      301 gattatgctt gatgctgatg ccttcgtttc gaaaattgcg attaatttcg tacacacgtt
      361 cgaagccacc tactactaac cgttttaggt aaagctctgg cgctattctc atgaacaagg
      421 tcatgtcgag cgcattgtga tgcgttttaa aagggcgagc agccgcaccg ccagcaatcg
      481 gatgcatcat cggtgtctca acttccaaaa aatctctttc gtccaaaaac cggcgaatcg
      541 cggccactat cttggtacgt gccgaaaaag ttttacgagc cgcttcgttc atgaccaaat
      601 cgagagaacg atgacgatat cggagctcaa catccgaaat gccatgaaat ttttctggca
      661 acgggcgcaa ggcctttgtc aaaaactcaa aacgttccgc acgcaccgaa agttcattgg
      721 ttttcgttct aaagactgtc ccttcaatat aaacaatatc acccaagtcg gtgaattctt
      781 tataccgatc gaacacatcc gctgaaagca cgtccttaga gagaaaaatt tgaatctggc
      841 cggagcgatc ctgaaggcgc aagaaaccac ccttaccgaa atctctgacc gccatgattc
      901 gcccagcaac cgaagctctg ggagcaacac tctctaagtt ttccttgggg agttcgccat
      961 atttctggtg aatttgggcg gcggtgtggt ctggagtcaa tccgttttta aatggattga
     1021 ttcccttttc aatcattcgg ttggcttttt cgaaccgaac ttcgatctgc tcattccagt
     1081 cagaaatatt tggtttcttc attcgtgaag gccttcaagg tagcgctcgg catctaatgc
     1141 agccatgcaa ccagtcccag cagcagttac tgcttgacga tatacatggt cctgcacgtc
     1201 tcctgccgca aacactcctg ggagattggt ctttgttcca ttttgggtaa caatgtaacc
     1261 gttcgcatca aggctaagtt gcttgttaaa gattcgtgta ttgggagcgt gcccaattgc
     1321 gataaacaag cctgtaactt taaggtcagt caccgcgccg gtttttaagt tcttcaatcg
     1381 cacgccagtg acatctgcat caccgagaac ttcctcgaca gccgaatccc aaatcatttg
     1441 aatttttgga ttcttagcga ccctgtcggc cataatttta ctagcgcgca actgatctcg
     1501 ccgatggacc atataaactt tcgacgcaaa acgagttaaa
//
LOCUS       DAOMXO010000621         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48267_length_1538_cov_4.584626, whole genome shotgun sequence.
ACCESSION   DAOMXO010000621 DAOMXO010000000
VERSION     DAOMXO010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48267_length_1538_cov_4.584626"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1538)
                     /locus_tag="PLH57_10955"
     CDS             complement(<1..>1538)
                     /locus_tag="PLH57_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRK03172.1"
                     /translation="LSLQDLEKRDDAIKILPETWHATYYHWMENIRDWCVSRQLWWGH
                     QIPAWHCKKCAHITVSRSDVTQCGQCGSSEVEQDSDVLDTWFSSGLWPFSTLGWPEKT
                     PALKTFYPTSVMETGFDILFFWVARMIMMGLHLNDGKVPFKRVYLHAMVRDEKGQKMS
                     KTKGNVIDPLDIIRDYGADAFRFTLTAMAGQGRDIKLSLDRIEGYKTFCNKLWNASRY
                     VLMRLGAVQDGDVSRVEAADFATVAGVTGGKPLTDWVQESFSKMHPVNRWILSRLQHT
                     TIAVEKGLEEYRLNDAASEIYHFVWNDYCDWYIEFSKELLASHPERVAETQICLLHVL
                     EQTLALAHPLIPFVTEEIYSYLPLRTGVKKGDALMLKTFPTAVAALIDETALRDIAVW
                     QEAITALRAFRGENNIPPKARPAVTFEGNEVEKGIGFIRALAQLDSLTRETGALKKNP
                     TTGQILAKHATFFVQLEGLVDFSGELKRLEKERSQIVADIEFVQQKLSKESFTAKAPP
                     ALVQ"
BASE COUNT          369 a          410 c          359 g          400 t
ORIGIN      
        1 ttttgtacga gcgccggtgg tgccttggcc gtaaaacttt ctttggaaag cttttgctgc
       61 acaaactcaa tgtccgctac tatctgagag cgctcttttt ctaggcgttt gagttccccg
      121 ctgaaatcga ccaatccctc gagctgaaca aaaaacgtgg cgtgtttcgc caaaatttgc
      181 ccagttgtgg gatttttctt caaagcacca gtctcgcgcg tcaaactatc tagctgcgcc
      241 aacgcgcgta taaaaccaat tcccttttcc acttcgtttc cttcaaaggt aactgcggga
      301 cgagccttgg ggggaatgtt attttctcct cggaaggccc gcaaagcggt aatcgcttct
      361 tgccacacag caatatctcg aagtgctgtt tcgtcaatca gtgctgccac ggcagtggga
      421 aaagttttaa gcatgagcgc atcgcccttt tttacgccgg tccgcaaagg caggtaggag
      481 taaatttctt ccgtcacaaa cggaataagt gggtgcgcca acgccagcgt ttgctctaat
      541 acatgcaaga gacatatttg agtctcagcc actcgctcag ggtgagaagc gagtaactct
      601 tttgaaaact caatgtacca gtcacagtaa tcattccaca cgaaatggta aatttccgac
      661 gctgcatcat tgagccgata ctcttcgaga cccttttcta ctgcaatggt cgtgtgttgc
      721 agacgtgata ggatccaccg attaacggga tgcatcttag aaaaactttc ttgcacccaa
      781 tcggtcaatg gtttcccccc tgtcactcct gcaacagttg caaagtcagc tgcttcgaca
      841 cgagaaacat cgccatcttg aacggcgcct aatctcatga gcacgtaacg cgaggcgttc
      901 cacagtttat tacaaaaggt tttgtaaccc tcaattcgat cgagactcag ctttatatca
      961 cgtccctggc ccgccatcgc agtcaacgtg aagcggaaag catcggcccc gtagtcgcgt
     1021 ataatatcca gcgggtcgat cacgtttcct ttcgttttgc tcattttctg gcctttttcg
     1081 tcgcgcacca tcgcgtgcag gtagactcgc ttaaagggaa ccttaccgtc gttgaggtgc
     1141 aaacccatca tgatcatgcg cgcgacccag aaaaacaaaa tgtcgaaacc cgtctccatc
     1201 acgctggtgg ggtagaacgt tttaagtgcc ggtgtttttt caggccaacc cagagttgaa
     1261 aatggccaaa gccccgaact aaaccaggtg tcgagaacat cgctatcttg ttcaacttcg
     1321 ctcgagccac attgaccgca ttgagtgacg tcgctacgac tcaccgtgat gtgtgcacat
     1381 ttcttacagt gccaagcggg tatctggtgc ccccaccaga gctggcgcga aacgcaccag
     1441 tctcgaatgt tttccatcca gtggtagtaa gtcgcgtgcc aggtttcggg taaaattttg
     1501 atcgcgtcgt cgcgtttttc gaggtcctgt agcgaaag
//
LOCUS       DAOMXO010000622         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48273_length_1538_cov_4.082266, whole genome shotgun sequence.
ACCESSION   DAOMXO010000622 DAOMXO010000000
VERSION     DAOMXO010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48273_length_1538_cov_4.082266"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1538
                     /gene="pheT"
                     /locus_tag="PLH57_10960"
     CDS             <1..>1538
                     /gene="pheT"
                     /locus_tag="PLH57_10960"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRK03173.1"
                     /translation="GLEVEAVHDLSKGFEKVITAQIEARDKHPNADRLSVCRVNTGKE
                     TLQIVCGAQNMKAGDKVALAQIGAHLPINLKIEKSKIRDVESFGMLCSETELAIADKS
                     EGILILPANAPVGLPLAQYLERNDVVFTLNVTPNRGDALSHWGVARELAALLGQTLKQ
                     PTIQLKEDKTTTASKYKVSVESSGGTERCFQYHARAINGVKVGASPQWLKTALEAVGQ
                     RSINNVVDITNFILLELGQPLHAFDSDKLRGGAIFARSAKSGEKIPLLDGTEIQLTAE
                     DAVIADAERPVALAGVMGGGNSEVTETTKNILLEGAQFLPASVRKTARRFQKQTDSSY
                     RFERQVDARQVTYALDRAAQLIQEVAGGVVLAGRVSAYSKQGENLVKGPLRSIQLSID
                     EMNSFLGTELSRERTVEMLESIGFTGKGAGSGMLDIEVPSYRPDVQIVEDLYEEVVRV
                     LGYNSIVASVPVLSDLPATTSGAEKRFAAVENVKKSLARAGFNEALHFAFVSEELNKK
                     WGEG"
BASE COUNT          415 a          343 c          411 g          369 t
ORIGIN      
        1 gaggtttgga agtcgaagcg gtccacgatc tatcaaaagg gttcgaaaaa gtgatcaccg
       61 cgcaaattga agcgcgagac aaacatccga acgccgatag gctttcagtt tgtcgggtca
      121 acaccggcaa ggaaacgttg caaatagttt gtggcgctca gaatatgaag gcaggcgata
      181 aagttgcgct tgcgcagatt ggtgctcatt tacctattaa cttgaaaatc gaaaaatcta
      241 aaatcagaga cgtcgagtcg ttcggtatgc tttgctcaga gactgaacta gcaattgccg
      301 ataaaagtga gggtatttta attcttccag cgaatgcacc ggtaggcctg ccgctggcgc
      361 agtatcttga acgtaacgac gtcgttttta cgttaaacgt aaccccaaac cgaggagacg
      421 cgctatcgca ctggggagtg gctcgcgaat tagccgcact gctcggtcaa acgttaaagc
      481 aacccacgat tcaattaaaa gaagacaaaa caacaaccgc ctcaaaatat aaggtgagtg
      541 tagagtcctc tgggggcact gagcgctgct ttcagtacca tgcgcgcgcg atcaatggcg
      601 taaaagtcgg agcttcgccg cagtggctga aaaccgcgct cgaagcggtg gggcagcgtt
      661 caattaacaa cgtggttgat atcaccaatt tcattttact agaactcggt cagccgctac
      721 atgccttcga tagcgacaaa ttgcgcggcg gcgctatatt tgcgcgttcc gccaaatcgg
      781 gcgaaaaaat tccgttacta gatggtactg aaatccaact gactgccgaa gacgctgtga
      841 ttgccgatgc cgaacgtccg gtggctttag ccggtgtcat gggtggtggc aactccgaag
      901 tcactgagac taccaagaat attttgctcg agggcgctca gtttttaccg gcatcagttc
      961 gcaagaccgc tcggcgtttt caaaaacaaa ctgactcgtc ttaccggttt gaacggcaag
     1021 tcgacgcgcg gcaagtgact tacgcgcttg atcgcgcagc tcaactgatt caagaagtag
     1081 ccggcggtgt tgttttggca ggacgagtga gcgcctattc gaaacaaggt gaaaacctcg
     1141 ttaagggacc acttcgaagc attcaacttt cgattgatga aatgaatagt tttttaggca
     1201 ccgaactttc ccgtgaacga acagtagaaa tgcttgagtc gattggattt actggaaaag
     1261 gcgctggtag tggcatgtta gacattgagg tgccgtctta tcggcccgac gttcaaattg
     1321 tagaagactt gtacgaagag gttgtgcgag ttttgggtta caactccatt gttgccagcg
     1381 tgccagtatt aagtgattta ccggcgacga cttccggagc tgaaaagcgc tttgccgccg
     1441 ttgaaaatgt aaaaaagagt ctcgctcgtg cgggttttaa tgaagctctg cactttgcgt
     1501 ttgtatctga agaactaaat aaaaaatggg gcgagggt
//
LOCUS       DAOMXO010000623         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48412_length_1535_cov_4.164189, whole genome shotgun sequence.
ACCESSION   DAOMXO010000623 DAOMXO010000000
VERSION     DAOMXO010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48412_length_1535_cov_4.164189"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLH57_10965"
     CDS             complement(<1..621)
                     /locus_tag="PLH57_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRK03174.1"
                     /translation="MALKTALVTGASGFIGSSVIRALSAQGIRPRALMRATSSKENLV
                     GVEYDLALGDLSDVESLKRAVDGVSYIFHLAGCVAAKTTAEYFAHNAQGTENLAIAAR
                     QNDSLERFIYVSSLAAGGPVSTDTPRTESDSDSPISDYGTSKREGEERLRRHSAGKYN
                     ITVVRPPAVYGPRDRGIFEFVKLVKSGILPKFPARTKSREKYVSLIH"
     gene            779..>1535
                     /locus_tag="PLH57_10970"
     CDS             779..>1535
                     /locus_tag="PLH57_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK03175.1"
                     /translation="MVIRSYETPLGSPRVHPPKAIRGTALTILGCGASSGVPLLACKC
                     PVCTSKNKKNKRLRASVSIQAGKRTFLIDTSPDFRQQAHQNGLHWIDAVLFTHPHADH
                     IHGLDDLRGFNFLMAKSIDCYGNSWTIGTLHSRFDYIFKKTQVGGGKPQLNLHIIDKP
                     LKIQGVRVIPLELIHGRMPVLGYRINNVAYVTDLTYLPDSTFRQLRGLDVLVLDCLRP
                     EPHPTHLHVDSALRLATRIGAKRTFFTHMGHELE"
BASE COUNT          390 a          418 c          332 g          395 t
ORIGIN      
        1 atggatgaga ctcacatatt tttcacgact tttggttcgt gcgggaaatt ttggtaaaat
       61 tccgctcttt acaagtttta caaattcaaa aataccgcga tccctagggc cataaactgc
      121 aggagggcga accactgtga tattgtactt tcccgcagaa tgccgacgca atcgctcttc
      181 gccttcgcgt ttactcgtac cgtaatcaga tatgggacta tccgaatctg attctgtgcg
      241 tggcgtatca gtcgaaacag gtccgccggc ggcaagactg gaaacataaa taaatcgctc
      301 gagagaatcg ttttgtcgcg cggcgattgc taagttttcg gtgccctggg cattgtgagc
      361 gaagtattcg gcggtagttt tggctgcaac gcaaccggcc aaatggaaaa tataactcac
      421 tccgtctaca gcccgtttca gtgattcgac gtcggacaaa tcacccaacg ccaaatcata
      481 ttcgactcca acgagatttt ctttagaact ggtcgcccgc atcaatgctc gcgggcgaat
      541 tccctgagca gacagggctc gaatgacgct actcccaata aagccgcttg cgccagttac
      601 cagtgcagtt tttaaagcca ttttggtgtt ttatgtaacc caggcacaaa ggtcaatcgc
      661 attaccgccc actttgcccc cggagtaact catggcgcaa aaaactagct tttaaattat
      721 tttgaaaacc aacttctccg ccctttccag tttggaagaa atcactcata aattcagtat
      781 ggtcatacgc tcttacgaaa ccccactggg ctcacctcgt gtacatccgc ccaaagccat
      841 tcgcggtacc gcgcttacca ttcttgggtg cggagcttcg tcgggtgtac cgttgctcgc
      901 gtgtaaatgt cccgtctgca cttcgaagaa caaaaaaaat aaaaggctgc gagcctcagt
      961 ctctattcag gcgggaaaac gcacattctt aatagacacc tcacccgatt ttcgccaaca
     1021 ggcacatcaa aacgggcttc attggataga tgccgtgttg ttcactcatc ctcacgccga
     1081 tcatattcat ggcttggacg accttcgcgg gtttaacttt ctaatggcca aaagcattga
     1141 ctgttacggc aactcctgga cgatcggcac ccttcatagt cgtttcgact acatctttaa
     1201 gaagactcaa gtcggcgggg gcaaacctca actcaattta cacatcatcg ataaaccgct
     1261 taaaattcaa ggcgttcggg ttattccgct tgaactcatt cacggtcgca tgccggtcct
     1321 aggataccgt atcaataatg tcgcctatgt taccgatcta acctacctgc cagattcgac
     1381 ctttcgacaa cttagggggc tcgatgtctt ggtactcgac tgtctgcgcc cagagcccca
     1441 tcccacgcat cttcatgttg acagcgccct acgactcgcg acacgtattg gagccaaacg
     1501 aacttttttc acccatatgg gacacgaact cgagt
//
LOCUS       DAOMXO010000624         1528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48760_length_1528_cov_3.767142, whole genome shotgun sequence.
ACCESSION   DAOMXO010000624 DAOMXO010000000
VERSION     DAOMXO010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48760_length_1528_cov_3.767142"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..>1528
                     /locus_tag="PLH57_10975"
     CDS             133..>1528
                     /locus_tag="PLH57_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK03176.1"
                     /translation="MSNKEVTVPVSTHSNALKPGDAAASPQRQNSLVPTTSALRRRSR
                     FSWIQKNSFYYKDLEKLFSFHVPKNGKVVEIGHGVGRLLSGCVPAQGLGIELDLSTVE
                     EARSHTEYPVEYWDGTSELPSTVLHQMSEGVDTILLVNTVGYWSDIEVALKKMAPLCR
                     PHTRIIAVYYNFLWAFPFRIAQKLRLKMPQADQNWLSAADVANLFRLSNYRVVKQGYR
                     CLMPVNLGPISWFMNRVLGNLPGLSRLGCTHFVIARPEHKIEPKNVFASVIIPARNEQ
                     GNIESAVRRMAQIASERKEKYEIVFVEGNSTDDTWGEIQRVINDPSIPKPFPVRAFKQ
                     PGKGKGDACRKGYAHADGNLFLILDADLTVPPEDLPKFVDAYCDGHGEFINGCRLVYK
                     MEREAMRPLNLLGNKFFSYAFTWLLGQRFKDTLCGTKVLSRDNYRRLEEGRAYFGDFD
                     PFGDFDLIFGASKLN"
BASE COUNT          399 a          343 c          366 g          420 t
ORIGIN      
        1 taaaatcgtg taaaaagata ttttagatag atacatgaga ccccctaatg actagtcctt
       61 tttataaatc cttcctatct tttcgtaatt agattgcgaa caagattgtg tcagggcaat
      121 gtttccgaga aaatgagtaa caaagaagta acagtgccag tatcgaccca ttcaaacgca
      181 cttaagccgg gagacgccgc agcttcgccg caacgccaga attcgctggt tccgaccaca
      241 tcagcgctgc gccgtcgatc gcgattttcc tggattcaaa aaaattcttt ttattacaaa
      301 gatttagaaa aactgttttc atttcatgtg cccaaaaacg gaaaagttgt ggaaattggg
      361 catggcgtgg gtcgcttgct ttcagggtgc gtgcctgccc aaggattggg tattgagctc
      421 gatctttcga ccgtagaaga ggcacggtct cataccgagt atccagtcga atattgggat
      481 ggcacatctg agcttccatc gactgtgctg caccaaatgt cagaaggagt ggacaccatt
      541 ttgctcgtta atacggtggg ctattggtca gatattgaag tggccttaaa aaaaatggcg
      601 ccgctatgcc gcccgcacac tcgaattatt gcggtgtatt acaatttttt atgggctttt
      661 ccgtttagaa tcgcccaaaa actacgttta aaaatgcctc aagccgatca aaactggctc
      721 tcggctgccg acgttgctaa tctctttcga ctctcgaatt accgagtagt aaaacagggc
      781 tatcgttgtt tgatgccagt gaacttgggt cccatttctt ggtttatgaa tcgagtattg
      841 ggcaatctcc cagggctttc gcgactcgga tgcactcact ttgtgattgc gcgacctgaa
      901 cacaagattg aaccgaaaaa tgtgtttgct tcggtcatta ttccagcgcg caatgagcaa
      961 ggtaatatcg agtccgcggt gcgtcgtatg gctcaaattg ccagtgaaag aaaagagaag
     1021 tacgaaatcg tttttgttga gggaaattca accgacgata cctggggcga aattcaacgg
     1081 gtgattaacg acccatctat tcctaaaccg tttccggttc gggcctttaa gcagccaggc
     1141 aagggtaaag gcgatgcttg cagaaaaggc tatgcgcacg cagacggtaa cttgttttta
     1201 attctcgacg ccgaccttac ggtaccaccg gaagatcttc caaagtttgt agacgcctat
     1261 tgcgacgggc acggcgaatt catcaatggt tgtcgtttgg tttataaaat ggagcgcgaa
     1321 gcgatgcgcc cccttaattt gcttggtaat aaatttttta gttacgcctt cacctggcta
     1381 ttgggtcagc gtttcaagga cacgttgtgt gggaccaaag ttttgagccg tgataactat
     1441 cgtcgtttag aagaaggccg cgcctatttc ggtgacttcg acccattcgg agatttcgat
     1501 ctcattttcg gcgcctccaa actgaacc
//
LOCUS       DAOMXO010000625         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48791_length_1527_cov_4.298913, whole genome shotgun sequence.
ACCESSION   DAOMXO010000625 DAOMXO010000000
VERSION     DAOMXO010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48791_length_1527_cov_4.298913"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /gene="pgk"
                     /locus_tag="PLH57_10980"
     CDS             complement(<1..166)
                     /gene="pgk"
                     /locus_tag="PLH57_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRK03177.1"
                     /translation="MIKCIDEIEIKDKRVLIRVDFNVPLSNATDPSNKDEPRKVTDGT
                     RIREALPTIQY"
     gene            complement(168..1175)
                     /gene="gap"
                     /locus_tag="PLH57_10985"
     CDS             complement(168..1175)
                     /gene="gap"
                     /locus_tag="PLH57_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010196833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRK03178.1"
                     /translation="MPIQVAINGFGRIGRRVLRAALKSSRIRVVAVNDLSSPETLAHL
                     FHYDSVHGRFPSEIEVRDGYLRVGTQEIKLTAERDPKNLPWKTLGVDLALECTGIFTD
                     KAQASAHIAAGAKKVVISAPSKDADLTVCVGINEKSYDSKNHHIVSNASCTTNCLAPV
                     VKVLDEAFGVQRGLMTTVHSYTNDQNILDLPHKDLRRARAAALSQIPTTTGAAKAIGL
                     VLPHLKGKLDGFSVRVPTPNVSLVDLTAELGKATTRDEINAKFAEAARGPLKGILAYS
                     TAPLVSCDYNGNEHSSTIDAELTQVMGGNLVKVVAWYDNESGFSCRMVELAEYMAGHL
                     I"
     gene            1305..>1527
                     /locus_tag="PLH57_10990"
     CDS             1305..>1527
                     /locus_tag="PLH57_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03179.1"
                     /translation="MLDAMNAVWTAFWGSLLETSPYLLLGFLFAGILHVLVPSKIIFW
                     ALGGRGIGGAIRGALLGIGIDSRELTLLEC"
BASE COUNT          355 a          353 c          403 g          416 t
ORIGIN      
        1 catactgaat cgtaggaagc gcttcacgaa ttcgcgtgcc atccgtgact ttgcgaggct
       61 catctttgtt agaagggtcc gtagcgttcg aaagtgggac gttaaagtcg acgcgaatga
      121 gtacgcgttt gtcctttatt tcgatttcat caatgcattt aatcatatta aattaggtgt
      181 cctgccatgt attcggctaa ttcgaccatg cggcacgaaa agccgctttc gttgtcatac
      241 caagcgacga cttttaccag gttcccaccc atgacttggg tgagctcggc gtcgatcgta
      301 ctggagtgtt cgttaccatt gtaatcacac gatactaagg gtgcggtgct gtaggctaag
      361 atacctttga gcggcccacg agcggcttcg gcaaacttgg cattaatttc gtcgcgagta
      421 gtcgctttac cgagttctgc agtgaggtcg acgagtgaaa cgttcggagt tggaactcgg
      481 accgaaaacc cgtcgagttt acccttcagg tgcggcaata caagtccaat ggctttggca
      541 gccccagtcg ttgtcggaat ttgcgacaaa gctgcggctc gggcgcgacg aaggtctttg
      601 tgaggcagat ctagaatgtt ttggtcgtta gtataggaat gaaccgttgt cattagaccg
      661 cgttgcacgc caaacgcttc atctaacact ttgaccaccg gagcaaggca gttggtggta
      721 caagacgcgt tcgaaacgat gtgatggttt tttgagtcat aggatttctc attaattccc
      781 acgcaaacag taaggtcggc gtctttgcta ggagcggaaa tcacaacttt tttggcgcca
      841 gcagcaatgt gcgccgaagc ctgtgcttta tcggtaaaaa ttccagtgca ttcaagggct
      901 agatccaccc caagtgtttt ccacggaagg tttttgggat cgcgttctgc agtgagctta
      961 atttcctgag tgccgactcg caaatagcca tctcgtactt cgatttcgct tggaaagcga
     1021 ccgtggaccg aatcgtaatg gaacaaatga gcaagtgttt cgggggagct cagatcgttc
     1081 accgctacta cacgaatgcg gctactttta agagcggctc tcaaaactcg gcgaccgatt
     1141 cgcccaaacc cgttgatggc tacctgaatt ggcatattcg aaagtcctcc gataatacga
     1201 aaaaattcaa ataaaagcgt gatttcaacg aaatttatag atcaaatcgc gagtccttcc
     1261 aagacttatt acagtttgtt aggctgttgc tgagggtcgc cgcaatgctc gatgctatga
     1321 acgccgtgtg gactgcattt tgggggagtt tacttgaaac ttcgccgtat ttgcttttgg
     1381 gattcctgtt tgcggggatt ttgcatgttc tggttccttc caaaattatt ttttgggcct
     1441 taggtggccg gggaatcggt ggcgcaatac gcggtgcgct gctcggcatc ggcatcgaca
     1501 gccgtgaact cacgctgctc gagtgcg
//
LOCUS       DAOMXO010000626         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48797_length_1527_cov_3.790082, whole genome shotgun sequence.
ACCESSION   DAOMXO010000626 DAOMXO010000000
VERSION     DAOMXO010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48797_length_1527_cov_3.790082"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PLH57_10995"
     CDS             <1..529
                     /locus_tag="PLH57_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03180.1"
                     /translation="YALGTIGQGGSVWSGVGGGAKYFYVDPTASLAHLRSVFRIELAR
                     NSSLEGESLFSDLEVSVAVIERMKRYAVPAELWREVLPFATGLEMSPRSSEVQLQFAK
                     ALIDLEAQRKAGIRLDLVRKDIRGARLLQGVSRYLVRPLYLGAIGAGTLAILYYVGSE
                     AVDYFQLVWRPGNSD"
     gene            complement(533..1312)
                     /locus_tag="PLH57_11000"
     CDS             complement(533..1312)
                     /locus_tag="PLH57_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03181.1"
                     /translation="MTVLRTLLFFSLFSVAGAAATSHRAFAGKIFAFPHLLPSPFTLP
                     AGQLSYGTEVALGITDFFQVGTNILRDAYRIFNANARLCMIDLPAFAFSPFVSFESFN
                     YRHIDSSNPDLRVTSWMPGAVAAFEISQTFALFTGGNVNITKTPLIEQNLTTSALLKG
                     ATIQSDLAWAYQAVGYGAAAPKKIKSTEDIEPDRLPKPIRGKHLGNVLSGGVSYDLNY
                     KLFGLGISHHWQGFHLGVHYYPKASKFPLQPIVSGGGSLDL"
     gene            complement(1335..>1527)
                     /locus_tag="PLH57_11005"
     CDS             complement(1335..>1527)
                     /locus_tag="PLH57_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03182.1"
                     /translation="HLVLVTKNAEALKVRLKEKECTIQYAPGIDKSESIKTEDREIAA
                     FPIAIKEERIRIVRSDQLF"
BASE COUNT          382 a          351 c          398 g          396 t
ORIGIN      
        1 atatgcgcta ggaaccatag gccagggggg ttcggtctgg tccggtgttg gcggtggtgc
       61 aaaatatttt tatgtcgacc cgaccgcttc ccttgcccac ttgcgatcgg tatttaggat
      121 agaactcgcc aggaactcga gcctggaagg agagtcgctg ttttccgact tggaggtttc
      181 cgttgcggtt atcgaacgaa tgaaacgcta cgcggtgcct gccgagcttt ggcgcgaagt
      241 attgccgttt gctactggtc tagagatgtc tcctagatcg agcgaagttc aattgcagtt
      301 cgctaaagcg ctcatagatt tggaggccca aaggaaagcc ggaattcgat tagacctcgt
      361 gcgaaaagac attcgaggag ctcgactttt gcagggcgta tctcggtatt tggtgcggcc
      421 gctgtatctt ggcgccatcg gtgccggtac gttggcaata ctttattatg tgggcagcga
      481 ggcggtcgat tattttcagc ttgtctggcg ccccgggaat tcagattaaa acctacagat
      541 ctaacgaccc gccacctgag acaatcggtt gcagcggaaa cttcgaggcc ttcggatagt
      601 aatgcactcc caagtgaaaa ccctgccagt ggtgactaat tcccaatcca aaaagtttgt
      661 agtttaaatc gtaactcaca ccaccagaga gtacgtttcc gagatgtttg cctcgtattg
      721 gtttgggaag cctgtcgggt tcgatatctt cagtactttt tatttttttt ggtgccgccg
      781 cgccgtaccc tacagcctga taggcccaag caagatccga ttgtatggtc gcgcccttta
      841 gaagcgcact ggtcgtaagg ttttgttcaa tcaatggggt cttggtaata tttacgtttc
      901 caccggtgaa gagtgcaaat gtttgcgata tttcaaacgc ggccactgca ccaggcatcc
      961 aagacgtaac ccgcagatct ggattggacg aatcgatgtg ccggtagttg aaactctcaa
     1021 aactcacgaa cggcgaaaaa gcgaaagccg gtagatcgat catgcaaagt cgcgcgttag
     1081 cattaaatat tcggtatgcg tcacgaagaa tgttggtacc aacttgaaaa aaatcggtga
     1141 taccgagcgc tacttcggtg ccgtaggaaa gttggcctgc gggcaaggta aaaggagacg
     1201 gtagtaggtg agggaaagca aaaattttac ctgcaaacgc tcgatgcgag gttgcggccg
     1261 ctcccgcgac cgagaataaa gaaaaaaata acaaagttcg taaaactgtc atgccaaaat
     1321 cctctgaatg tcatttaaaa caattgatcg gatcggacaa tccgaatgcg ttcttctttt
     1381 atcgcaatag ggaatgccgc aatttctcgg tcttcggtct ttatcgactc ggatttgtcg
     1441 atacccggag cgtactgaat cgtacattct ttttctttca accgaacctt tagcgcctcg
     1501 gcgttcttgg tgaccaaaac taaatgt
//
LOCUS       DAOMXO010000627         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48890_length_1525_cov_3.406122, whole genome shotgun sequence.
ACCESSION   DAOMXO010000627 DAOMXO010000000
VERSION     DAOMXO010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48890_length_1525_cov_3.406122"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..>1525)
                     /locus_tag="PLH57_11010"
     CDS             complement(123..>1525)
                     /locus_tag="PLH57_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03183.1"
                     /translation="DEDLPSPYELEGIGVCFKDKSNCDPRPENVVVRAIVDDPMASDL
                     AIRPRYEWFPPLNPEQTARACQRLWNQLTNGSFDGLANWLLSNRIKLPSCADQKLADR
                     VVDELIVKFKKNLYGGSFQFDHLEELLRGLTMLEQLGANYDRDVGLGSDGLLEYFGDC
                     SRNLTSKNEKTFPSDCGYREKNEKNATFNLMFKSAYRDLVRAHWRQVQHRRSTRDCAV
                     VEQKIEKEFQKNRTGVQKFLTELFKPIFVSRKASLAEDRKRLKAMAKSKSEKECRAEA
                     LKFIGEWPQELKETMMFKVCQPNLRKIYRSHPSSAMKAFQKRVGTNFESATNYLCANA
                     QSNWWQFENVAESIVEDLHTIITFEPSNDDGFDFFSEGSDSCSEKSESYRKTDARVEY
                     NLHLGVNFEYEVSSHDQSEPKSVDRQFEEQLKQNLSALHLDSLARYKARWYPHCVKAG
                     QPARLAPIESDSRAAP"
BASE COUNT          370 a          376 c          337 g          442 t
ORIGIN      
        1 gtttgatcga gtagatcatg cagatccccc gacttgaatt ctaacgattc gatttaaaaa
       61 aagcaaaggt ccacgcgtac ttgcgttagg aagcgctaga ttcacaagac ccactcacat
      121 ttttacggcg ctgcgcgcga gtcagattca ataggggcaa ggcgcgcggg ttgcccagcc
      181 ttcacgcaat gggggtacca acgcgccttg tagcgagcca gtgaatcgag atgcagcgca
      241 ctaaggtttt gttttaactg ctcttcaaat tggcgatcta ccgattttgg ttcgctctgg
      301 tcgtgggaac tcacctcgta ctcaaagtta acccccagat gaagattgta ctctacgcgc
      361 gcgtcagtct ttcggtaact ctcgcttttc tctgaacagc tatcgctgcc ttctgaaaag
      421 aaatcgaacc cgtcatcatt cgaaggttcg aaagttatga tggtgtgaag gtcctcaact
      481 atgctttcgg ctacgttctc aaattgccac caattggact gggcattcgc gcataaataa
      541 ttggtcgccg actcgaaatt agtgcctact cttttttgaa acgctttcat cgctgaacta
      601 gggtggctcc ggtaaatttt tcgcaaattt ggctgacaca ccttaaacat catcgtttct
      661 tttagttctt ggggccattc accaatgaac tttaaagcct ccgcgcgaca ttctttttca
      721 ctcttacttt tggccatggc cttgagtctc ttgcgatcct cggcaagact tgctttgcgg
      781 gaaacgaata ttggtttaaa cagttcagtg agaaattttt gaacccccgt tcgattcttt
      841 tgaaactctt tttcaatttt ttgttcgacg accgcacaat cacgcgtgct tcgccgatgc
      901 tgaacctgcc tccaatgagc gcgtaccaag tctcgatatg ctgacttaaa cattaaattg
      961 aacgtcgcat ttttttcgtt cttctcgcga taaccacagt cggagggaaa agttttttca
     1021 tttttagaag taagattccg tgagcaatca ccaaagtatt ccaataaacc atctgagccc
     1081 aaacccacgt cacggtcgta gtttgcccct agctgttcta acatagtcag tccgcgcaaa
     1141 agttcttcta gatgatcgaa ttgaaacgaa cctccatata gatttttttt gaactttacg
     1201 atcaattcgt caacgactcg gtcggctagt ttttgatctg cgcagctcgg cagtttaatg
     1261 cgattgctga ggagccaatt ggccaggccg tcaaacgacc cgttcgtgag ttgattccat
     1321 agtcgttggc aggcccgagc agtttgttct ggattaagcg gcgggaacca ttcgtatcgg
     1381 gggcgaatgg ctaaatccga agccatcgga tcgtcgacaa tggcgcggac tactacgttc
     1441 tcggggcgtg gatcacaatt acttttatct ttaaaacaaa ccccgatgcc ttccaattcg
     1501 taaggactcg gtaaatcttc gtctt
//
LOCUS       DAOMXO010000628         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_48893_length_1525_cov_3.265306, whole genome shotgun sequence.
ACCESSION   DAOMXO010000628 DAOMXO010000000
VERSION     DAOMXO010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48893_length_1525_cov_3.265306"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLH57_11015"
     CDS             complement(<1..618)
                     /locus_tag="PLH57_11015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HRK03184.1"
                     /translation="MAVGAAALLYTLALHAFRPLARTSWSDIWALRKNSAHLGPSLLH
                     GVIIGAITLIATASGGYLSYLGFYLRVDEIALSSLTIFFFSAAVLMTALVEEYIFRAC
                     LEPVLSKRLSLGSTIAISTAMYVCTKAFLFDISAVVALNLALLNISFSLFARARGTFV
                     ASALLYFGLFTTMHFCFGLPFFGQDMPGILLLRPDSASSEPGTLGY"
     gene            902..>1525
                     /locus_tag="PLH57_11020"
     CDS             902..>1525
                     /locus_tag="PLH57_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK03185.1"
                     /translation="MIALTSVSKTYPQSAGPVFDNVTLKINKGEFLYIVGDSGAGKTT
                     LLKMLCGEETPTRGTVQVFGEDISKMAFEKVQATRRRIGVIFQELRLVEDLTAYDNVA
                     LALHAAGDTERYSKRAIDEALALVNLSRLAHKKVAGFSGGEKQRVAAARAIVRQPEIL
                     LADEPTGSLDRDHTWSLMDLFQKLHLRGTTILVATHDREIVPRVRRRS"
BASE COUNT          430 a          344 c          416 g          335 t
ORIGIN      
        1 ataccccagt gtaccaggtt ccgaactcgc gctgtcgggg cgaagcagca atattcctgg
       61 catgtcttgt ccgaagaacg gcaatccgaa acaaaagtgc atggtggtga agagtccgaa
      121 atacaagagc gcagatgcaa caaaagtgcc tcgcgcccgc gcaaacagtg aaaaactaat
      181 gttaagaaga gccaagttca gcgccactac ggctgaaata tcgaacaaga aagccttggt
      241 gcagacatac atcgcggtcg aaattgcgat ggtgctgcca agagaaagcc ttttgctgag
      301 cacgggctcg agacatgctc taaaaatata ttcctctacg agtgcggtca ttaaaaccgc
      361 tgcagaaaaa aagaagatag ttaagcttga aagggcaatt tcgtcgactc gaaggtaaaa
      421 cccgagataa cttagatagc cgccactagc ggtggcgatg agggtaattg cgccaatgat
      481 tacgccgtga agaaggctgg gacccaagtg agcggaattt tttcgcaatg cccatatgtc
      541 gctccagctc gttcgggcta aagggcgaaa cgcgtgtagg gccaaggtgt aaagtaaagc
      601 ggcggcgcca acagccaata tttcgttggc tgcaattaag tcctgaattt attgcaggct
      661 atcgcgctcg ataccggttt cctggcgtac aaaaatgtag gtcaccatcg taaggacaac
      721 caaatttaca gccttctgag tgaaaacgta gcccaaccaa agaaataatg ttcgtgcgac
      781 catgaaattg agtgtagctg gagttgccgg gcgaaggggg aaaaaaacga tctagggaag
      841 gattttcccg ctctgctcgg agcgccggat ctttgctact ttgaagtgag gagatgtcgg
      901 aatgattgct ctaacgagtg tgtccaaaac ttatccgcaa agtgcaggtc cggtgtttga
      961 caacgtgacc ttaaaaatta acaaaggcga attcctctac attgtaggtg atagtggagc
     1021 gggaaaaacc actttactca aaatgttgtg cggtgaggaa actcctaccc gtggaactgt
     1081 tcaggttttc ggcgaggaca tatcgaaaat ggcttttgaa aaagttcagg ccacccgacg
     1141 gcgtatcgga gtaatttttc aagagctacg actggtcgaa gacctcacgg cctacgacaa
     1201 tgttgctctt gcattgcacg cggcgggaga taccgaacgg tactcgaaga gggcaatcga
     1261 tgaggcgctg gcgctcgtga acctttcgcg cctggcccac aaaaaggttg cgggtttttc
     1321 tgggggcgaa aaacaacgag tggcggctgc gcgcgctatc gtgcggcagc cagaaatatt
     1381 gttggcagat gaaccgaccg gtagtttaga tcgagatcac acttggagtc ttatggattt
     1441 atttcaaaaa ctccatttac gcggcacgac catattagta gccactcacg atcgggaaat
     1501 cgttccgcga gtgcgtcgtc ggtct
//
LOCUS       DAOMXO010000629         1497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_50210_length_1497_cov_4.192094, whole genome shotgun sequence.
ACCESSION   DAOMXO010000629 DAOMXO010000000
VERSION     DAOMXO010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50210_length_1497_cov_4.192094"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLH57_11025"
     CDS             <1..798
                     /locus_tag="PLH57_11025"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HRK03186.1"
                     /translation="TSPFEFTGNKFEFRAVGSSSPISFPITVLNTAVAQAMGETTDLL
                     KKKLKAGRPIDAAILEVVRETLLETKPVRFEGNNYSDDWVREAERRGLPNLKRAPEAM
                     EQLLSPSSKTLFSETGVLREQELHSRYHVMMERHVKKLTIEAETLATMVDTMIVPAAL
                     KEQKALGEAIRALTEVTRNADVSEQTTRLGVVSQGINDLLSKRKDLTAMVARVQNLTE
                     EAAKAHMLSGEVATLMSQIRSISDRLEDLVSDEFWPLPKYREMLFIS"
     gene            801..>1497
                     /locus_tag="PLH57_11030"
     CDS             801..>1497
                     /locus_tag="PLH57_11030"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HRK03187.1"
                     /translation="MDVLKGYREIKKSFRDAVLTVGNFDGLHLGHQSILNEVVARAKS
                     LRGQSIVYTFRPHPQAVLSPSRDLELISTYDEKLRLFENAGIDVVIEEPFSREFSGFS
                     AEKFFSDVIVKSIAPRAVFVGYDFGFGKDRSGSLDILKKMCKTEGIDLSIASPLKIEN
                     EVCSSSRVRAYLKSGDIVSANRLLGRRFSYQGSVIRGDARGRTIGFPTANLLASAKIQ
                     IKNGVYASGTIVTM"
BASE COUNT          424 a          301 c          371 g          401 t
ORIGIN      
        1 acctcgcctt ttgaatttac gggtaataag tttgagtttc gagcagtcgg ttctagttcc
       61 ccgatttctt ttccgatcac tgttttgaat actgcggtgg cgcaagcaat gggtgaaact
      121 accgacctgt taaaaaagaa acttaaagca ggtaggccga ttgacgccgc aattctcgaa
      181 gtggttcgcg agaccctgct agaaactaaa ccagtgcgat ttgagggcaa caattactcc
      241 gatgattggg tacgcgaagc cgaaagacgc ggtttgccta atttaaagcg agcccctgag
      301 gcaatggaac agttgctgtc accatcgtct aagacgcttt tttcggaaac tggagttttg
      361 cgcgagcaag agttgcattc gcgttatcac gtaatgatgg agcggcacgt aaaaaaactc
      421 acgatcgaag ccgaaacctt ggcgaccatg gttgacacaa tgattgtacc tgctgcgctt
      481 aaggaacaaa aagcattggg tgaagcaatt cgagcgttga ctgaagtgac tcgcaatgcc
      541 gacgtgtcag aacagactac gagattaggt gtggtttcgc agggaattaa cgacctatta
      601 agtaaacgaa aagatctcac tgcgatggtt gctcgagtgc agaacctcac agaagaggca
      661 gctaaagccc atatgttgag tggcgaggtc gctactttga tgtcgcaaat tcgttcgatt
      721 agcgaccgac tcgaagatct cgtatccgat gagttttggc cgctgcctaa ataccgcgaa
      781 atgctgttta tttcttaagt atggatgtgc tcaagggtta tcgcgaaatt aagaaatcct
      841 ttcgtgatgc agtattgacc gttggaaatt tcgacggttt acaccttggg caccaaagta
      901 ttttaaacga agtagttgcc agagcgaaaa gcttgcgtgg tcaaagtatc gtttacacat
      961 ttcgtccgca cccacaggca gttttgtcgc cctcgcgaga tctcgaatta atcagcactt
     1021 acgacgaaaa attaagactt ttcgaaaacg caggtatcga cgtagtaata gaagaaccct
     1081 ttagtagaga attttcgggt ttttcagcgg aaaaattttt ttctgacgtc atcgtaaaat
     1141 caattgcacc gagagccgta tttgttggct acgactttgg ctttggaaaa gaccggtcag
     1201 gaagtttaga tattttaaaa aaaatgtgta aaacagaagg aatcgatctt tcgattgctt
     1261 caccattaaa aattgaaaac gaagtgtgtt cgagttcacg cgttagagct tatttaaaaa
     1321 gtggcgatat cgtttcagcg aaccgtttat tggggcgacg tttctcttat caggggtctg
     1381 tcattcgagg cgacgctcgt ggtcgcacta tagggtttcc aacggcaaac ttgttggcaa
     1441 gcgcgaaaat tcaaattaaa aatggcgttt atgcgagtgg gactatcgta acgatga
//
LOCUS       DAOMXO010000630         1496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_50252_length_1496_cov_3.916724, whole genome shotgun sequence.
ACCESSION   DAOMXO010000630 DAOMXO010000000
VERSION     DAOMXO010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50252_length_1496_cov_3.916724"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..964)
                     /locus_tag="PLH57_11035"
     CDS             complement(23..964)
                     /locus_tag="PLH57_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK03188.1"
                     /translation="MIEVQALSKFYGTRKAVDELSFNVEKGEVLGFLGPNGAGKSTTM
                     KILTCFMPATSGTAKVAGYDVFEQPLEVKKRVGYLPETPPVYKDMVVLDYLIYAANLH
                     GLKKAAAKNAALEAIEKCGLGGVKGRLIGNLSKGYRQRVGLAQAIVHRPEVLILDEPT
                     VGLDPKQIIEIRGLIKSLAGDHTVILSTHILPEVQATCSRVLIINQGRIVAIDSLDAI
                     SARLRKGNVLSLVVERGTSDLVGKIQSLPGVASVSNPERADGGHRIIVTADESSDIRS
                     AVAKIAFSSDAGLLELKRESMSLEDVFVKLTTVEHSI"
     gene            complement(1037..>1496)
                     /locus_tag="PLH57_11040"
     CDS             complement(1037..>1496)
                     /locus_tag="PLH57_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03189.1"
                     /translation="LHYFIVKKPKAKEGFEQEGGMVAFATLSAGEAQRAVKDELLRWG
                     QSVDFDVTEPCMLVLNLHSKAGFKQALKTLVNNATMVAKAAAPEGIAPQFSLVFVPTS
                     DASVARSLKSLGFENVFTSEKIQIYRISNQAAKSPDSRDLDNSSPAKAVF"
BASE COUNT          367 a          371 c          350 g          408 t
ORIGIN      
        1 gtttcgcata aaaacctcca gtttaaatcg aatgctccac agtcgtaagt tttacgaaaa
       61 cgtcctcgag cgacattgat tctcttttaa gctccaacag acctgcgtct gaggaaaatg
      121 cgattttagc gaccgctgaa cgaatgtctg aagattcatc ggcagtaacg ataattcggt
      181 gtccaccatc cgctctttca gggttggaaa cggaggcaac gccaggaagt gattgaatct
      241 ttccaaccaa gtctgacgtt ccacgttcaa caaccaaact aagaacatta cctttgcgga
      301 gtcgagcgga tatcgcgtcg agtgaatcga tcgctacgat ccttccttgg ttgatgatca
      361 aaacacggga acatgtcgct tgtacttctg gtagaatgtg cgtgctaaga ataacggtat
      421 ggtcaccggc caaacttttt atcagacccc ggatttcaat gatttgtttt gggtctagac
      481 cgaccgttgg ttcatctaaa attaacactt cgggtcgatg cacaatggcc tgcgcaagcc
      541 ccactcgttg tcgatatccc ttggaaagat tgccgattag gcgcccttta actccgccta
      601 ggccacattt ttcaattgct tcaagcgccg catttttcgc ggccgccttc tttaaaccat
      661 ggagattggc tgcatagatc aaatagtcta acaccaccat gtctttataa acgggcggtg
      721 tctcagggag gtagcccacg cgttttttta cttcgagcgg ttgttcgaag acgtcatacc
      781 ccgccacctt cgccgttcca ctggtggctg gcataaaaca ggtgagaatt ttcattgttg
      841 tggatttacc cgcgccgttg gggccgagaa agcctagaac ctctcctttt tccacgttaa
      901 aggaaagctc gtcgaccgcc ttacgagtgc cgtagaactt ggacaatgcc tgaacttcga
      961 tcatagacgc tcccagtcga attcaaattg aatttgtacg cttcaattta gacgggagag
     1021 cttttttgtc aagacctcag aaaactgcct tggcgggact ggagttgtcc aagtcccggg
     1081 agtctggcga cttggccgct tggtttgaaa tccggtagat ttgaattttt tcggaggtaa
     1141 acacattctc gaatccgagg ctctttaagc tgcgagcaac tgaggcgtca gaagtcggca
     1201 cgaacacgag actaaattgt ggcgcgattc cttcgggggc ggccgctttt gctaccattg
     1261 tagcattatt cacaagtgtt ttgagtgcct gcttaaatcc agccttagag tgcagattta
     1321 atacgagcat acaaggttcg gtaacgtcga aatccacgga ttgcccccaa cgcaacaatt
     1381 cgtctttcac ggcccgttgt gcttcacctg ctgatagcgt ggcaaatgcg accataccgc
     1441 cttcttgctc gaaaccttct ttagcctttg gttttttaac aatgaaatag tgaagc
//
LOCUS       DAOMXO010000631         1495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_50301_length_1495_cov_4.215972, whole genome shotgun sequence.
ACCESSION   DAOMXO010000631 DAOMXO010000000
VERSION     DAOMXO010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50301_length_1495_cov_4.215972"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLH57_11045"
     CDS             complement(<1..397)
                     /locus_tag="PLH57_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03190.1"
                     /translation="MKKLTQIFRIFSPGVLSGFCIVGVVLVGVVSSGCPTATSTTACS
                     EEYVDPSDRRACNSFCSTASNSKKLNKIDDLEDFKAGCLKGQRGFLSSQVSLKVDQIH
                     AFCDDVENQSSSWKVGCLVGASREWLQKNY"
     gene            complement(586..1125)
                     /locus_tag="PLH57_11050"
     CDS             complement(586..1125)
                     /locus_tag="PLH57_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK03191.1"
                     /translation="MRHPNMTSILMTVEELAKYLKIKPDTIYKKVRRGELPALKLGKL
                     VRFPKDLIDQWVIDQATRTMKDVKAAKKAVGAKVEEAFGEVKKTARTAGQLIDQVRHA
                     PMNKKQDALNKGLKKLLKEVDQGIKKFTPKSPKARARRGASPKVVRKTKTAKSARAVK
                     MTTAPEEHTSSGTPATLTH"
     gene            complement(1230..>1495)
                     /locus_tag="PLH57_11055"
     CDS             complement(1230..>1495)
                     /locus_tag="PLH57_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRK03192.1"
                     /translation="YISLVLKILFGFGLAFELPVLLVLFAKLGIVTPSSMRAHRRTAL
                     IVITIVAAFVAPPDAVSMLLMMAPLYVLYEGAIFVISKLVRSS"
BASE COUNT          373 a          357 c          338 g          427 t
ORIGIN      
        1 cgtagttctt ctgcagccac tcacgcgacg cacccaccaa acaaccgacc ttccaggagg
       61 aagactggtt ttcgacgtcg tcacaaaatg cgtgaatctg atctactttc aaacttacct
      121 gagagcttag aaacccccgt tgtcccttaa gacagccggc cttaaagtct tctaagtcat
      181 cgattttatt taactttttt gagttactgg cggtactgca aaatgaatta caagctctgc
      241 ggtccgaggg gtcaacatac tcttcactgc aagcggtggt ggaagtcgcc gtcggacatc
      301 cgcttgaaac aacccctacc aggaccaccc caacgatgca aaaaccgcta agaacgccgg
      361 gcgaaaatat tctaaaaatt tgtgtcagtt ttttcacgat gtaccccttt tttgacatgg
      421 ggcaggagta aatcattttg cggtgcattt caatctagga gctacgaatt tggtgggttt
      481 ttggtggggg cttcaaaaac cggcctgtag gggcgtttga gtggagggga ctaatcctac
      541 aggccggttc gaagatcaaa acgcttatgg cgctttgaaa accttttaat gcgtcaacgt
      601 tgctggcgta ccgcttgaag tgtgttcttc aggggcggtg gtcattttaa ctgctcttgc
      661 agacttcgca gtcttggtct tgcgaactac ctttggcgaa gcgccgcgcc tggctcgagc
      721 tttaggggac ttaggtgtga atttttttat cccttgatcg acttccttta gcagcttttt
      781 tagaccctta tttagagcat cttgcttctt attcatggga gcgtgtcgca cctgatcaat
      841 taattgaccc gcggtgcgtg cggtcttttt aacttcaccg aacgcttctt caaccttagc
      901 gcccactgcc tttttcgcgg ctttcacatc tttcattgtg cgggtcgctt gatcgattac
      961 ccactgatca atcaaatctt tcggaaaccg gaccagctta ccaagcttta gagcgggcaa
     1021 ttctcctcgg cgcacttttt tgtaaatcgt gtctggctta attttaagat atttcgccaa
     1081 ctcctctaca gtcatcaaaa tgctggtcat atttggatgt cgcatgtggt gaactccctt
     1141 aactatattt ataccaatcg ttttttatgg gggcaagctt ttattagtat taattagtac
     1201 taatcagtat ctagtatgct tgagtatggt tagctggacc gaaccaactt ggagattacg
     1261 aagattgctc cctcgtacaa aacgtacagt ggggccatca ttaacagcat cgaaacagcg
     1321 tcgggtggcg ctacgaacgc cgctacgata gtaataacga tcaaagcggt tcttcggtgt
     1381 gcacgcatgg aagacggcgt tacaattcca agcttagcga acaacaccaa taaaactggc
     1441 aattcgaacg ccagtccaaa accaaagaga atcttgagta ctaaagaaat atagc
//
LOCUS       DAOMXO010000632         1490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_50525_length_1490_cov_5.379791, whole genome shotgun sequence.
ACCESSION   DAOMXO010000632 DAOMXO010000000
VERSION     DAOMXO010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50525_length_1490_cov_5.379791"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLH57_11060"
     CDS             <1..512
                     /locus_tag="PLH57_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK03193.1"
                     /translation="SKGMLQRVGFAQALLHDPAVLILDEPMSGLDPVGRREVRDIIAD
                     IAKTGKTIFFSTHIIHDVEVLCTTVGFIQHGKLKGSGNIDSLLGKTVKSVEIRFSMAA
                     DRDASKVNTLRNSRKTMDGWVLELESDVEKIEQQTAEIISSILDEKGRVKSIIPRKST
                     LEDLFFGET"
     gene            509..1312
                     /locus_tag="PLH57_11065"
     CDS             509..1312
                     /locus_tag="PLH57_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRK03194.1"
                     /translation="MKQNYLRTPIKQSLRQVFAISSVTFQELLREKILWSALLFAVLC
                     VALSLAAAQLSFADQARIALDFGLTSVSIIGGLISVIMGSSLIAKEVHHRTLYLVLTK
                     AVWRWQFVVGKFLGLLALLTLNSLIISGIAIGIFLLLDGQFRMSILQNYILQMSEFAI
                     LSAVACVFSAFSTATLSAIVTSGIWIIGHAMTDFQVLIPKIEPPLARPFLDFTARVLP
                     DLTRFDIKAQVSHILPVTWSYTGVSVLYALSYVLFALAIACFVFTRRDL"
     gene            1309..>1490
                     /locus_tag="PLH57_11070"
     CDS             1309..>1490
                     /locus_tag="PLH57_11070"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRK03195.1"
                     /translation="MSLSPLLDRLAIGWFGLIGLVVGSFLNVVIYRLPRGYLLSLPRR
                     SVCANCKKQIRGYENIP"
BASE COUNT          354 a          332 c          339 g          465 t
ORIGIN      
        1 tttcaaaagg aatgctccaa cgcgtcgggt ttgcccaggc ccttttacac gacccagcgg
       61 tattgatttt agatgaacca atgagcgggc ttgaccccgt tggtcggcgt gaagtgcgcg
      121 acatcattgc cgacattgcc aaaactggga aaaccatttt ttttagcact cacattatcc
      181 acgatgtgga agttttgtgt acaaccgttg ggtttattca gcatggcaaa cttaaaggct
      241 ctggcaatat cgatagctta ctcgggaaaa ccgtaaaatc tgttgaaatt cgattttcta
      301 tggctgccga tcgcgatgct tcaaaagtta atacgcttcg caacagtcga aaaactatgg
      361 atggttgggt attggaactt gaatcggacg tcgaaaaaat cgaacaacaa acggcagaaa
      421 taattagcag tattttggat gaaaaaggcc gagttaagtc gataataccc cgaaaaagta
      481 cccttgaaga tctctttttt ggtgaaacat gaagcaaaat tatcttcgaa cgcctatcaa
      541 gcagtcactt cgccaggtat ttgcaatttc ctcggttacg tttcaagaac tgctacgcga
      601 aaaaattctt tggagcgcgt tactatttgc agttttgtgt gttgcgctat ccctcgcggc
      661 agcacagctt tccttcgccg atcaagcgcg gattgcgctg gactttggac ttactagcgt
      721 aagtataatt ggcggcctaa tctcagtgat catgggttct tcgttgatcg ccaaagaggt
      781 gcatcatcgc actctttatc tggtgctcac gaaggctgtt tggcgttggc aatttgtagt
      841 tggcaagttc ttgggactgc tagccttact aactctaaat tcactcatta tcagtggcat
      901 cgcgatcgga atttttctat tactcgatgg ccagtttcgc atgtcgattt tgcaaaacta
      961 tattttgcag atgtcagaat ttgctattct ttcagccgtg gcctgtgtgt tttccgcgtt
     1021 ttccacggcg actctttcag ccattgtgac ctcgggaatc tggatcattg gtcacgccat
     1081 gactgatttt caagtattga ttcctaaaat agagccgcct ttagcgcggc cttttttaga
     1141 ctttacggcc cgcgttcttc ccgatctcac gcggtttgat atcaaagcgc aagtctccca
     1201 tattttaccg gtaacttggt cctacactgg cgttagcgtg ctctatgctt tgtcctacgt
     1261 tctatttgcc ctagctatag cctgttttgt ttttacgagg cgtgatttgt gagtctctcc
     1321 cccttactcg atcgattagc gatcggttgg ttcgggttga ttggtttagt ggtgggtagt
     1381 ttcctaaacg tagtgattta ccgcttaccg cgagggtacc tacttagtct tcctcgacgt
     1441 tcagtttgcg caaattgtaa aaaacaaatt cgaggttatg aaaatattcc
//
LOCUS       DAOMXO010000633         1489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_50582_length_1489_cov_3.650628, whole genome shotgun sequence.
ACCESSION   DAOMXO010000633 DAOMXO010000000
VERSION     DAOMXO010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50582_length_1489_cov_3.650628"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLH57_11075"
     CDS             complement(<1..816)
                     /locus_tag="PLH57_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK03196.1"
                     /translation="MTSVKKYQESFFLTIGTLAVLSAIVLGIGDSPISVASVLLNSAF
                     GSLENFSYTLFYSTPILLTACSVRLALAAGLFNIGAEGQMLIGALASVAFGLWFGPTL
                     PVFVVIPSAIVFAMAAAAFWAGIAGWLRVRHGIHEVISTILLNFVALALVNWSVLEIF
                     KNPENQSLETTWIAEGLRFGPLWKHATVFAPLAIGLAVLVHAVLTTTWFGLRVRSTGH
                     NPRASELAGIAATKIRLKSLLIAGAIAGLAGVNEVFGHAYRLIDGFSPGYGFIG"
     gene            complement(813..>1489)
                     /locus_tag="PLH57_11080"
     CDS             complement(813..>1489)
                     /locus_tag="PLH57_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HRK03197.1"
                     /translation="GNGQDELVGLITRQNLKFEHQGIGVKLFGNELRECDTRTVRSRD
                     LAIVPADRTHDGLVLELSAAENLLLGKKQCGASDFSRIPVQTLETSCLNQMREFDVRP
                     TEPGLPVGAFSGGNQQKLILARELAGITRNGFILAAHPTRGVDLGAIEFIHRQLLDRA
                     ESGAGILLISSELSELFALCHKIYVLYKNELRGPFFRDPRNFSFDSEVIGQAMTGLSQ
                     KRAQVS"
BASE COUNT          423 a          360 c          347 g          359 t
ORIGIN      
        1 cccgatgaat ccgtagccag gagaaaaccc gtctataaga cgataagcat gaccgaacac
       61 ttcgttcacg ccggcgagcc ctgcaattgc acctgctatc aataggcttt ttaatcgaat
      121 tttagtggca gcaatgccgg caagttcgga agctcgtgga ttatgccctg tcgaacggac
      181 gcggagtcca aaccaagtgg tggtgagtac ggcatgtact aatacggcga gtccaattgc
      241 gagaggtgca aaaacggttg cgtgtttcca aagcgggcca aagcgcagac cctcggcgat
      301 ccaagtggtt tcgaggctct gattttctgg gtttttaaaa atttctagca ccgaccaatt
      361 tacaagggct aaagctacga aattcaatag aatggttgaa attacttcgt gaataccgtg
      421 gcgaactcgc aaccaacccg cgataccagc ccaaaatgcc gcggccgcca tagcgaatac
      481 tattgcactg ggaattacaa cgaaaactgg aagcgttggt ccaaaccaaa gtccaaacgc
      541 gacagacgcc aaagccccga tcaacatttg tccttcggcg ccaatattga ataatccagc
      601 cgcgagagct aatcgcacgc tgcatgcggt tagtaaaatt ggagtggaat aaaacaaagt
      661 atatgaaaaa ttttccaggg acccaaacgc ggaattaaga agtacgctgg cgacactaat
      721 cgggctatca ccaattccta gcacaatggc cgaaagcaca gccaacgttc caatagttaa
      781 aaaaaaggac tcttgatatt ttttcacgct cgtcacgata cttgcgccct tttttgagaa
      841 agcccagtca ttgcttgacc gataacttca gagtcgaatg aaaaattacg tggatcgcga
      901 aaaaaaggtc ctctcaactc gtttttgtag agaacataaa ttttgtgaca gagcgcgaac
      961 agctctgaaa gttcactaga aattagcaaa attcccgctc cgctttcggc gcgatcgagc
     1021 aattgacggt gaataaactc aatagcgccc aggtcgaccc ctcgagttgg gtgcgcagcg
     1081 agaatgaaac catttctcgt aattcctgcc agttctcgag ctaagatgag tttctgttgg
     1141 ttacctccgg aaaatgctcc aacaggaagg cctggctcag ttggtcgtac gtcaaattcc
     1201 ctcatttgat tgaggcaact ggtttctagt gtctgcactg gaattcgcga aaaatcactg
     1261 gcaccacact gtttttttcc caacaaaaga ttttcagctg cagaaagttc gagaactaat
     1321 ccgtcgtggg tacggtcggc cggtacgatc gctaagtctc gagagcgaac agttcttgtg
     1381 tcgcactcgc gtagttcgtt accgaaaagc ttgactccga taccttgatg ttcaaatttt
     1441 aaattttggc gggtaataag gcctaccagt tcgtcttgtc cgttgcctt
//
LOCUS       DAOMXO010000634         1488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_50619_length_1488_cov_5.165387, whole genome shotgun sequence.
ACCESSION   DAOMXO010000634 DAOMXO010000000
VERSION     DAOMXO010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50619_length_1488_cov_5.165387"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLH57_11085"
     CDS             <1..694
                     /locus_tag="PLH57_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRK03198.1"
                     /translation="SKSPFSNPVWRAHQWLVWKRTLSGFIRNRHGRRIVPWKRRRRWR
                     RMLLKFPLPAVRSNPTSKAGRKMALDFARRIATAKQRVWIANAYFVPTRMIRNALTRA
                     CDRGVDVRVMVPKSPDIFFIRWVSAALTASLAKCGVRIFEYGPSVLHAKYALIDDWVT
                     VGSTNLNHRSFLFDYELDVVLSSPESRKSIEEHFLIDSHRSRELDRNFCEQVPWIERL
                     LGRFLLRLRKWM"
     gene            685..1335
                     /locus_tag="PLH57_11090"
     CDS             685..1335
                     /locus_tag="PLH57_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRK03199.1"
                     /translation="MDVIQSVRRFNGTTVPQPSDHIATQLHAAVVHAKRFELPLDFEI
                     GCGAGMHPIEYNQSEKQRVLIAVEHTRIKYQAFCQHLLKNQQVDLRRLVPLHANAISV
                     LTHLVPPASIERIYLLYPNPEPKARNRRWFCAPFMAKVLDCLVPGGTVHVASNIDWYF
                     NEALHQARNVWGLQVTAERSFNENTIPFSKPRTHFEKKYLERGQICYDAILLKPNR"
BASE COUNT          373 a          367 c          365 g          383 t
ORIGIN      
        1 tagcaaatcc cctttttcga atcctgtatg gcgcgcacac caatggctgg tgtggaaacg
       61 cacgctcagc ggattcattc gcaatcgcca cggaagacgt attgttccgt ggaaacggcg
      121 ccgaaggtgg cgacgcatgc ttcttaaatt tcctttgccc gcagtgcgtt cgaacccgac
      181 aagcaaggcg ggtcgaaaaa tggcgctcga tttcgcaaga cgaatcgcca ccgcgaaaca
      241 acgggtttgg atagcgaatg cctattttgt tcctactcgc atgattcgca acgctctcac
      301 ccgggcttgc gaccgcggcg tggatgtgcg cgtaatggta cctaagagcc cagatatttt
      361 ttttatacgg tgggtgagtg ccgctcttac agctagtctt gccaaatgcg gagtgcgaat
      421 ttttgagtac ggcccttctg ttttacatgc taagtacgcg ctcatcgatg attgggtaac
      481 ggtgggttcc acaaacttga atcatcggag ttttttattc gactacgaac tcgacgtcgt
      541 actgagttct cctgagtcac gcaaatcaat agaagaacat tttttaattg attcgcaccg
      601 ctcccgggaa ctcgaccgaa atttttgtga acaagtgcct tggatcgagc gactcctcgg
      661 ccgtttttta ttaaggctaa ggaaatggat gtaatccagt cggttcgacg atttaatggt
      721 acgacggtgc cgcagccgtc cgaccacata gcgactcaat tgcatgcggc cgttgtgcac
      781 gcaaagagat tcgaattgcc attagacttc gaaatcggat gtggtgctgg aatgcaccct
      841 atcgaataca accagagtga aaaacaacgg gtcctgatag cagtcgaaca cacgcgaatt
      901 aaatatcagg cgttttgcca acatctctta aagaaccaac aggtagatct acggagactg
      961 gtaccacttc atgcaaatgc aatttcagtc ttaacacacc tggttccgcc agcttcgatt
     1021 gagaggatat atctacttta cccaaaccct gaacccaagg cccgcaaccg tcgttggttc
     1081 tgcgcaccgt tcatggccaa agttctagat tgcctagtgc ctggcggaac tgtgcatgtg
     1141 gctagcaata tcgattggta ttttaatgaa gctttgcatc aggctcgcaa cgtttggggg
     1201 ctgcaagtca ctgctgaacg atcgttcaac gaaaacacga ttccgttttc gaaacctcga
     1261 acgcacttcg aaaaaaaata tcttgagcgc gggcaaattt gttacgacgc gattttactc
     1321 aagccgaaca ggtagcgtta cgaccgatga gtttttgtta gctttaaatc ggtactgcgt
     1381 cgtgaaatca cgcaccaact tcacgatatt acgttcggcg ttcgctacgg aacttcttag
     1441 agtgctgggc agggtgtgga acttttgcac aagtccgctt gcgtccac
//
LOCUS       DAOMXO010000635         1474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_51290_length_1474_cov_4.590557, whole genome shotgun sequence.
ACCESSION   DAOMXO010000635 DAOMXO010000000
VERSION     DAOMXO010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51290_length_1474_cov_4.590557"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLH57_11095"
     CDS             <1..256
                     /locus_tag="PLH57_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03200.1"
                     /translation="RALSGHENEDHPKTPRKTLEPLSLEATNGALSYTGTVFKDHVTK
                     RFSTDFIPLEIGTNKIRLDFNYPFSKIFQLEINVERKDRN"
     gene            complement(273..1193)
                     /locus_tag="PLH57_11100"
     CDS             complement(273..1193)
                     /locus_tag="PLH57_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03201.1"
                     /translation="MKDRIYWALSAIATFACLLTMTGTPTYAREVAALPDASSTVVGP
                     QPETSLSVLKPISQVGQSGDYIDQVPVPEASRSFRAEIFDDTLTRELVERNAFNIRYE
                     ERGNYQLNHVEDYHRWLSHNQEVAEWAVRKLIHYHLEKNLIPRIEKSLQKKASEKSSS
                     PGQSAAQAANTVYGVHKALKNTKINFGAETSGKFRFDMPKGLLEFVIRSPLVDTDFQY
                     KAYGRRTGNVLGSASQQSERMSVGMRRSLSLIGGGVAHARYGLVSDTLSYGISRQIVG
                     PISAQVDQSRHFSESHRDEFVYRVNFGANF"
     gene            complement(1323..1454)
                     /locus_tag="PLH57_11105"
     CDS             complement(1323..1454)
                     /locus_tag="PLH57_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03202.1"
                     /translation="MVLLGGFAGAVSELLPLELDDNFTIPVVSGFILWLGFIAMQFI"
BASE COUNT          413 a          380 c          335 g          346 t
ORIGIN      
        1 tcgcgcgtta tccggacatg agaatgaaga ccatccaaaa acaccgcgaa aaacactcga
       61 gcctctgtct ctcgaggcga ccaatggtgc cttgtcctat accggcaccg tatttaaaga
      121 ccatgtcacc aaacgatttt ctaccgactt tattccgtta gaaattggaa ccaacaaaat
      181 aagactagat ttcaattatc cgttttcaaa aatttttcag ttagaaataa acgtcgaacg
      241 aaaagaccgc aattaagcgg ctgcacccaa cactaaaaat ttgcaccgaa attcacacgg
      301 taaacaaact cgtcacggtg agactctgaa aaatgcctcg actggtcgac ttgcgcgcta
      361 atcggaccaa ctatctggcg actaataccg tagcttagcg tatcgctcac aaggccatac
      421 ctcgcatgag caactcctcc gccgatcaaa gacagcgaac ggcgcattcc cacgctcatt
      481 cgctctgatt gctgggacgc cgaaccaaga acattgcctg tccgacgtcc ataggccttg
      541 tactggaagt cggtatctac caagggtgaa cgaatcacga actctagtaa tcccttaggc
      601 atgtcgaacc ggaactttcc gctcgtttcc gcaccaaagt taattttggt atttttgagg
      661 gccttgtgaa cgccatagac cgtgttggct gcttgagcgg ccgactggcc gggggaagaa
      721 cttttttcac tggccttttt ctgcaaactt ttttcaattc taggaattag atttttttct
      781 aagtggtagt gaatcaattt tcgcacagcc cattcagcca cttcctgatt gtgagaaagc
      841 caacgatgat aatcttcgac gtggtttaac tggtagttgc cgcgctcttc gtagcgaata
      901 ttgaatgcgt ttcgctcaac aagttcccga gttagagtgt cgtcaaaaat ttccgcgcga
      961 aaagatcgcg aggcctcggg gaccgggacc tgatcgatgt aatcgccact ttgcccgacc
     1021 tgcgaaatag gcttcaacac cgaaagcgag gtttctggtt ggggccccac aacggtgctg
     1081 ctggcatccg gcaaggcggc aacctcacga gcgtacgtag gggttcctgt catcgttaga
     1141 agacaagcaa aagtcgcaat agctgaaagg gcccaataaa tgcggtcctt cacgagcaag
     1201 acacctccta ggaggtatgc aatgcaggtt ccagaccatc acggcggcta aaaaaagcct
     1261 ttaatttaag gggcttggta aaatttatat gtcgatattt taagcaggcg cgaagggttt
     1321 gatcaaataa actgcatggc tataaacccg agccacagaa tgaacccgct caccacgggg
     1381 atcgtgaagt tgtcgtcgag ttcgagcgga agcaactcgg aaacagctcc agcaaagccc
     1441 cccaacagta ccactaatta cactaaccct caaa
//
LOCUS       DAOMXO010000636         1471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_51433_length_1471_cov_4.718927, whole genome shotgun sequence.
ACCESSION   DAOMXO010000636 DAOMXO010000000
VERSION     DAOMXO010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51433_length_1471_cov_4.718927"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PLH57_11110"
     CDS             <1..1197
                     /locus_tag="PLH57_11110"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRK03203.1"
                     /translation="AHFLFLLILFLVGMACYRSTTSLEFAFKYLSSFSGLIFIPWAFE
                     QLTKVSRPERSNFELFKWLTLLSITAVILGVLLQAAGLVPWRSYDLIPPSYFGHAFAE
                     EPLKKVWRLTGFYYHPLDLIRVLVWPYFFLVLLASRLPENRRTVPILLFAGMHLIFIG
                     NTHRMTLFFLLTSTVFLFWYLGSAKFSAYGLLIVLVSWLIGILLFSAVHRTNFSHIFA
                     PSQFINVPKLALKFNYRLKSITTDPPVLNAPIVPAIPLEKQQIITLRGRGYFWKNHFN
                     RTLEFSAAEWLFGTSKAFIGSPNSPDGVWNDSQPHSQVLDALARFGVIGLLLILAFYI
                     YVFGFQFKLGPIAGSLALGTLAWYGIFTEVLIMPTFSWWALLFVWFVSKPNLLCARNL
                     TFKRLD"
     gene            complement(1159..>1471)
                     /locus_tag="PLH57_11115"
     CDS             complement(1159..>1471)
                     /locus_tag="PLH57_11115"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRK03204.1"
                     /translation="ITVQFEGPTGPQVITETFDVSETGAFVPAGQLLRSRLKPNVGTP
                     CVVILKIDGVGEFQLHGRVARCAEGNGSYPAGIGLRFEDVPNLRRRTLVEALEGQVAS
                     A"
BASE COUNT          340 a          343 c          312 g          476 t
ORIGIN      
        1 gctcactttt tgtttctttt gattttgttt ttggttggga tggcttgtta ccgtagcact
       61 acttctcttg agtttgcgtt taagtaccta tcaagcttct caggtttaat ttttattccc
      121 tgggcgtttg agcagttaac aaaagtcagt cgcccagagc gtagtaactt tgaattattt
      181 aagtggctta cacttctttc aatcactgct gtcattctcg gcgtactgtt acaagcggcg
      241 ggactggttc cttggcgatc gtatgattta ataccaccct cttatttcgg tcatgctttc
      301 gctgaagagc cattaaaaaa agtgtggcgt ttgacgggct tctactatca cccgctcgac
      361 ttgattcgag tattagtttg gccttatttt ttcttagtac ttttagcttc gaggcttccc
      421 gaaaataggc gaacagtacc aattttgttg tttgctggta tgcacctaat ttttattggc
      481 aacactcacc gtatgacctt attttttttg ctgacttcca ctgtttttct tttttggtac
      541 ttaggaagcg cgaagttttc cgcctatggt ctgctaatcg ttttagtgag ttggctaatt
      601 gggattttac tattttcggc agtgcatcgc acaaactttt cccacatctt tgcaccgtct
      661 caattcatca atgtacctaa gttagcttta aaatttaatt accgattaaa atctattacg
      721 actgatccac cagttttaaa tgccccgatt gttcccgcca tcccgctaga gaaacaacag
      781 attattactc tgagaggccg cggctatttc tggaaaaatc actttaatag aacgctagag
      841 tttagcgcgg cggagtggct ttttggcact tcgaaagctt ttattggcag cccaaactca
      901 ccagacgggg tctggaacga ctcccaaccc cacagtcagg tgcttgatgc actcgctcga
      961 ttcggagtga tcggactgct attaatttta gcattttata tttacgtatt tggttttcaa
     1021 tttaaattgg gaccaattgc cggctcgctc gcacttggca ctttagcctg gtacggaata
     1081 ttcacagagg ttttgatcat gcccaccttc agctggtggg ccttactctt cgtctggttt
     1141 gtttcaaaac caaatttact atgcgctcgc aacttgacct tcaagcgcct cgactaaagt
     1201 gcggcgccga agatttggaa cgtcttcgaa tcgtagcccg attccagccg gataggagcc
     1261 atttccttcg gcacatcggg caacgcgacc atgaagctga aactctccta caccatcaat
     1321 ttttagaata actacacatg gagttcccac atttggcttc aatcgagagc gaagcaattg
     1381 gcccgcggga acaaaagccc cggtttcgga aacatcaaat gtttcggtga tgacctgagg
     1441 acccgtgggg ccttcgaact gcacggtaat g
//
LOCUS       DAOMXO010000637         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_51498_length_1470_cov_4.306007, whole genome shotgun sequence.
ACCESSION   DAOMXO010000637 DAOMXO010000000
VERSION     DAOMXO010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51498_length_1470_cov_4.306007"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="PLH57_11120"
     CDS             <1..1435
                     /locus_tag="PLH57_11120"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HRK03205.1"
                     /translation="PFFGSGKSGRYELVDFDGAEGCRLNLHPSMTGVVSRDGQVMAVN
                     SESSAPVTLREKDFAKIKIKDMSFFLSFSPAPPRLKSARLGRRDPLFVRMLMLSIAST
                     VAIIATIAGVTVDPTIEIEQLPERVATIIYEPKFLPIERPPLPVAKTEPAPSVAPAPK
                     PPKVKIDVAEVKKPEEPAPKNAMIGVKPEQSKPVPKPPAPPKEASPKKQVVSGKAGGQ
                     EGAGAKAKGEEGGRGKPNAKPNPVAQTKIARPGDAKKNSGLSASPGQSQTNSMGVVDV
                     FKSNAGTLSKIFAGGKGASTAADKLEGYSGFTTRGEGGLGAAGTGAGGGGSSMGLGGL
                     ADKGPGGGKQGKGLGALGSGGNILGGKGTLAIDTGGSAEPIVLGAIDTSAIAREIDKH
                     RDEIKYCYEKEINADRPDLSGRVVMRFEIGASGSVSSAGVAATTLRHAATEQCIVDVI
                     KRIQFPPVAGGGETDVTFPFVFKPTNK"
BASE COUNT          374 a          349 c          432 g          315 t
ORIGIN      
        1 accatttttt ggttctggta agtccggtcg ctacgaactg gtagatttcg acggggccga
       61 aggatgtcgt ttgaatcttc atccaagcat gaccggggta gtgagtcgcg acggtcaggt
      121 gatggcagta aatagtgagt caagtgctcc agtcactttg cgcgaaaaag attttgcgaa
      181 aattaaaata aaagacatga gtttctttct tagtttttca cccgctccac cgcgtcttaa
      241 gtcggcgcga ctcgggcgac gcgacccctt gttcgtgaga atgttgatgc tctcgatcgc
      301 atcaaccgta gcgatcatcg ctacgatagc gggcgtaacg gttgatccca cgattgaaat
      361 cgagcagttg ccggaacgtg tcgccacaat aatttatgaa cccaagtttt tgccgatcga
      421 gcggccgccg ctcccagtag ccaaaactga gcccgcgcca tcggtggcgc cggcccctaa
      481 gccgcccaaa gttaaaattg acgtggccga agtaaaaaag ccggaagaac cggctcctaa
      541 aaacgcaatg atcggcgtga aaccagaaca gagtaaaccc gtaccgaaac ctcccgcgcc
      601 gccgaaagag gctagtccca aaaaacaagt tgtctccggt aaggcaggtg gacaagaagg
      661 tgcaggcgct aaggcaaaag gcgaggaagg cgggcgcgga aagcccaatg ctaagccgaa
      721 tccggttgcg caaactaaaa tcgctcggcc tggcgacgct aaaaagaact cgggtctttc
      781 ggcaagtccg gggcaatccc agaccaatag catgggtgtg gtggatgtgt ttaaatcgaa
      841 tgctggaaca ctttccaaga tttttgctgg tggaaagggt gcttcgaccg ctgcagacaa
      901 actcgaaggt tatagcggat ttacgacaag aggcgaaggt ggcctgggcg ctgccggaac
      961 aggtgctggc ggtggtggat ctagcatggg cttgggtggt ctggcggaca aaggtccggg
     1021 aggcggcaaa caaggaaagg gtctcggtgc tcttggttct ggcggcaaca ttttaggcgg
     1081 aaaaggaacc ttggcgatcg acactggcgg cagcgcagaa cctatcgtgc tcggcgcgat
     1141 cgatacttcg gcgatcgcgc gtgagataga caaacatcgc gatgagatta agtattgtta
     1201 cgaaaaagag atcaacgcgg atcggccaga tttaagcggt cgcgtggtga tgcgttttga
     1261 aattggggcc agcggtagcg tgagttcggc gggcgttgcg gccactacgt tgcgtcatgc
     1321 cgcgaccgaa caatgcattg ttgatgtcat taaacgcatt cagtttcccc ctgtagcggg
     1381 tgggggagaa accgacgtaa cctttccgtt cgtttttaaa ccgacaaaca aatgaacttc
     1441 gaacatccta gtattcagat ttttagtttc
//
LOCUS       DAOMXO010000638         1467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_51673_length_1467_cov_3.146601, whole genome shotgun sequence.
ACCESSION   DAOMXO010000638 DAOMXO010000000
VERSION     DAOMXO010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51673_length_1467_cov_3.146601"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PLH57_11125"
     CDS             <1..1065
                     /locus_tag="PLH57_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HRK03206.1"
                     /translation="ALDIFRRDIPASKKLTLVVMDDEGDVDRALAAMDDFFYKHQVAV
                     VIGPMVSRLGDPVGKKAQELGMPLISLTQKESAGGDYVFNVALTPEMQVKAIAAYATE
                     KLNLKRFAIMAPNSKFGSEYARTFWDELEARNAVVRGYETYPDGETDYRRFVDRIVGL
                     GENEARAREIEELNILKEAVPIKTRSKKLDRMFSLKPIVDFEAVFIPDEPKALGQILP
                     TFAYRDVENIVFLGINTWNSAELISRAGQFAENAVFVDGFYPGSQKTLAREFIEDFRR
                     TFNNEPGLLEALAFDAGRIAAQLLRVSMVPSRKEMRDRIMSLRNFDGVSGSISYRSGR
                     LSKDLHILTVKSGRIEEVAQ"
     gene            1196..>1467
                     /locus_tag="PLH57_11130"
     CDS             1196..>1467
                     /locus_tag="PLH57_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03207.1"
                     /translation="MEISNPLGNGKFKQVVRELVNAEREPIRQLEARKAKENERLKLF
                     QEFLGKMRKLPEVYKELDSFRKFRELKAEWPAKDQLDVLIDKDIAET"
BASE COUNT          394 a          357 c          371 g          345 t
ORIGIN      
        1 gctctcgata tattccgcag agacattccc gcatcaaaga aactcacgct ggtagttatg
       61 gacgacgaag gcgatgtgga tcgcgcgctc gctgcgatgg atgatttctt ttataaacac
      121 caagtagcag tggtaattgg tcccatggtt tctcgcctcg gagaccctgt tggcaagaaa
      181 gcgcaagagt tgggcatgcc attgatttcg ctcactcaaa aagagtcggc tggcggtgat
      241 tacgttttca acgtggcgct aactcccgaa atgcaggtca aagcgattgc cgcttatgct
      301 accgaaaaac ttaacctaaa acgctttgcc attatggcgc cgaactccaa gttcgggtcc
      361 gaatatgccc gcaccttttg ggatgaactc gaagcaagaa acgccgtagt gcgtggttat
      421 gaaacttatc ctgatggcga aacggactat cgacgcttcg tggatcgaat cgttgggctc
      481 ggggaaaatg aagctcgggc ccgcgaaatc gaggaactga atatactaaa agaagccgtt
      541 ccaattaaaa cgcgctccaa gaaactcgac cgaatgtttt cgcttaaacc gatcgtcgat
      601 ttcgaagccg ttttcattcc ggacgaaccc aaggcattgg ggcagatttt gcctaccttc
      661 gcttatcgag atgtagaaaa catcgtcttt ttaggaatca atacctggaa cagcgcagaa
      721 ctaatttcgc gggctggcca gttcgccgag aatgcggtgt ttgtggacgg attttatcct
      781 ggctcgcaaa aaactctcgc ccgcgaattt attgaggatt tccggagaac attcaacaat
      841 gagcccggtt tacttgaagc actagccttc gatgcagggc gaatcgccgc tcaactgttg
      901 cgagtttcaa tggtgccaag ccgtaaagaa atgcgcgacc gaatcatgtc actacgaaat
      961 tttgacggcg tctcgggaag catatcctac cgttccgggc gcctttcgaa ggaccttcac
     1021 attcttacgg taaagtccgg gcgaattgaa gaagttgccc agtaacgcgc accacccgtt
     1081 tgctcttccg acacccactc cactatactg atatctaagg ggcaaagtgt tttttggcct
     1141 ctggcgcggc acatgaggtg ctggcgtacg ccattatcag gaggatcggc tgagcgtgga
     1201 gatttctaac ccgcttggaa atgggaagtt caagcaagta gtacgcgagc tcgtcaacgc
     1261 ggagcgcgaa cctattcgcc agctcgaagc gcgtaaggcg aaggaaaacg aacgtcttaa
     1321 actatttcaa gaattcctcg gaaaaatgcg caagctgcct gaagtttaca aagaactcga
     1381 cagctttcga aaatttcgtg aattaaaagc cgaatggcct gctaaagacc agttagatgt
     1441 attgatcgac aaagatatcg ccgaaac
//
LOCUS       DAOMXO010000639         1463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_51901_length_1463_cov_2.365767, whole genome shotgun sequence.
ACCESSION   DAOMXO010000639 DAOMXO010000000
VERSION     DAOMXO010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51901_length_1463_cov_2.365767"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLH57_11135"
     CDS             <1..229
                     /locus_tag="PLH57_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03208.1"
                     /translation="FDRRVGVDGDLQETSTVICHRCQMPVLEEEQKLPSYVLGKSCLH
                     CVDGKVFHKEKIAESIALSSAHRHGDSTSPS"
     gene            264..>1463
                     /locus_tag="PLH57_11140"
     CDS             264..>1463
                     /locus_tag="PLH57_11140"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRK03209.1"
                     /translation="MAFITCLAVILTTSVSAHAKKRRKQRAEPVPVVSTHSKLLAGLP
                     HAMDVLANGLKIYAVKYPSPGVIAYQIPVRVGSRNEVLEGRTGFAHFFEHLMFRGTKK
                     RTGKEFGDLYTRLGVENNAWTWNDMTNYHGTASPRVFEQILEAEADRFMNLSFDEKLF
                     RDEAGAVLGEYNKNIANPENVIEEKLAELAFEKHTYGHTTMGYKSDIVKYGERYGDVW
                     PFFKRHYRPSNTAIVVVGDIDPKRTIQLVKKYFGPWRNPVLTAAALRAESVPPEPVQT
                     EARISSETLDKPAQTRLAIAYKIPPFSSENSDFAVLNAIAEIAFSDISPFVQKYRYEQ
                     KWLDSVYAPVIEMIDPGIWTVNLRLSNTGKPHTETLITEVQKTLNALASAAPSSSILT
                     EAKNRLMN"
BASE COUNT          420 a          358 c          333 g          352 t
ORIGIN      
        1 gtttgaccga cgagtcggcg tagacggcga cttacaggaa acaagtaccg tcatttgcca
       61 tcgctgccaa atgcccgtgc tcgaagagga acagaaatta ccttcgtatg tgctaggaaa
      121 aagctgtttg cactgcgtcg atggtaaagt gtttcataaa gaaaagattg cagagtcgat
      181 cgcattgagt tcagctcata gacatggcga ctcgacatca cccagttaga aacgttcgtt
      241 tattcactcg aaataagttt tggttggcgt ttatcacttg cctggcggtg attcttacca
      301 ccagtgtttc agctcacgca aaaaagcggc gcaaacaacg cgcggaaccc gtgcctgtgg
      361 tcagcaccca cagcaaactt ctagctggtt tgccacatgc gatggatgta ttagccaacg
      421 gcttaaaaat ttatgccgta aagtacccca gtcccggagt aatcgcctac caaattccag
      481 ttcgagtggg atcgcgcaac gaagtgctcg agggccgaac tggattcgca catttttttg
      541 aacacctaat gtttcgcggc accaaaaaac gcaccggtaa agagtttgga gacctctaca
      601 ccagacttgg agtagagaac aatgcttgga catggaacga catgaccaat tatcacggca
      661 cggcctcgcc tcgagtattc gaacagattt tagaggcaga agctgatcgt tttatgaatc
      721 tttcgttcga tgaaaagcta tttcgcgatg aagccggagc ggttctaggc gaatacaaca
      781 aaaacatcgc caaccctgaa aatgtaatcg aagaaaagtt ggcagaactc gcattcgaaa
      841 aacacaccta cgggcacact acgatgggat acaaatcaga catcgtgaaa tatggcgaac
      901 ggtacggcga cgtctggcca ttttttaaac ggcattatcg cccgtcgaac actgctatcg
      961 tagtggtcgg ggatattgac cccaagcgca ccattcaact ggttaagaaa tattttggtc
     1021 cctggagaaa tccggtccta actgcggctg ctcttcgcgc tgaatccgtg cctccggagc
     1081 ccgtacaaac tgaagcgcga attagcagcg aaaccttaga taaaccggct caaacccgtt
     1141 tggcgattgc ctataagatt cctccttttt cctcagaaaa ctccgatttc gcagtactta
     1201 atgcgattgc agaaattgct ttttctgaca tttccccttt tgttcaaaaa tatcgctacg
     1261 aacaaaagtg gttagattca gtttacgctc cggtaatcga aatgatcgac ccaggaattt
     1321 ggacggtgaa cctaaggttg tccaacaccg gaaaaccaca taccgaaacc ttgattaccg
     1381 aagttcagaa aacattaaac gcgttagctt ccgcagcgcc ttcgagttca attcttaccg
     1441 aggctaaaaa ccgtcttatg aac
//
LOCUS       DAOMXO010000640         1451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_52517_length_1451_cov_4.028653, whole genome shotgun sequence.
ACCESSION   DAOMXO010000640 DAOMXO010000000
VERSION     DAOMXO010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52517_length_1451_cov_4.028653"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1451)
                     /locus_tag="PLH57_11145"
     CDS             complement(<1..>1451)
                     /locus_tag="PLH57_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03210.1"
                     /translation="TITLCIGRIAKGQDLYGPPLPPGFVEPKTMSAAAPPAVPKSESI
                     GFWGQIKAALTSTIGRVIPGNGDSESSDYQKLDVSGRTPTSVSKISSPEADRKIVALV
                     NESIEQSNRLTRLLKDPSTLEFETTKPLLTIAAQEPALRIELHKKYSPESSPKESITF
                     ASDTFTNNARLLPLSDKAIIPVTAVHIDELKLVRALLAIVGDKNDNAGDQLHRLGTGT
                     KAEIVKLKSLAFLGHLLFEQNIPTLAATSLVRALDPSQNSPEGLKFNSYTLETLSKVA
                     RGQLSAKTLIRPNVFEKIIDHSGLSDEARSFASLVLAERAFDSGRFESARKLAQGIAP
                     KSSWSERGRYLAALCIYAAKPENRASQELAGRELTDLFRTVEDPAVFDAVATALGRIH
                     FILGNYKAANQYLEQVTKETNIWLESAVDNGWSLLKSGDKHHAVGNMFTLHTPYFEGA
                     YMPDSYFLQTLGYQELCQFGDAFTSVKKYKLMY"
BASE COUNT          328 a          326 c          389 g          408 t
ORIGIN      
        1 cgtacattaa tttatatttt tttaccgaag tgaacgcgtc accgaactga caaagctctt
       61 ggtacccaag ggtttgtaaa aagtaggagt caggcatgta agcaccctcg aagtaaggag
      121 tgtgcaatgt aaacatgttt cccactgcgt ggtgtttgtc gccggatttt agtaatgacc
      181 atccgttatc tactgccgat tcgagccata tattcgtttc tttggttact tgctcgagat
      241 actggttggc ggctttgtag ttgcctagaa tgaaatgaat gcgccccagt gcggtagcta
      301 cggcatcgaa aactgccggg tcttcaaccg ttctaaataa atcggtcaac tctcgcccgg
      361 cgagttcctg gctggctctg ttctcgggtt tggcagcata gatgcaaagc gcggcaagat
      421 agcgaccgcg ttcgctccag ctggatttag gagctatgcc ttgggccaat ttacgagcgg
      481 attcaaaacg accggaatca aacgcgcgct ccgccaacac gaggctggca aaacttctcg
      541 cttcgtcgga gaggccgcta tgatcgataa ttttttcgaa aacatttggg cgaattaaag
      601 ttttagcgct gagttgacct cgcgccactt tcgatagtgt ttcgagagtg taactgttga
      661 atttcagacc ctctggcgag ttttggctag ggtctagtgc tcgcactaga ctggtggcag
      721 cgagggttgg gatgttttgt tcgaataaca agtgtcccaa aaacgccagt gatttgagtt
      781 taacgatttc cgcctttgtt ccggtgccga ggcgatgcaa ttgatcgccg gcgttgtcgt
      841 ttttgtcacc cacgatagca agcagcgctc tcaccagttt taattcatct atgtggactg
      901 ccgtaaccgg aataatcgct ttatccgaca gcggcaatag tcgtgcgttg ttggtaaagg
      961 tatctgacgc gaacgtgatg ctttccttcg gcgaagattc aggactatat tttttgtgaa
     1021 gctcaatacg aagcgccggt tcttgagccg ctatcgtgag caatggtttt gtggtctcga
     1081 attccaaggt gctcgggtct ttgagtaagc gagtaaggcg gttgctttgt tcaatggatt
     1141 cattgacgag tgctacgatt ttgcgatcgg cctcggggct agagattttt gaaaccgaag
     1201 tgggcgttcg acccgataca tctagttttt gataatcaga agattcggaa tcgccgtttc
     1261 caggaataac ccggccaatt gtacttgtga gcgcagcttt gatttgaccc cagaatccta
     1321 tagattccga ttttggtacc gctggcgggg cggcggcact catagttttg ggttcaacaa
     1381 acccgggagg aagcggtggg ccatagagat cttggccttt cgcaatgcgc ccaatgcata
     1441 gcgttatcgt g
//
LOCUS       DAOMXO010000641         1447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_52723_length_1447_cov_4.433190, whole genome shotgun sequence.
ACCESSION   DAOMXO010000641 DAOMXO010000000
VERSION     DAOMXO010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52723_length_1447_cov_4.433190"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1447
                     /gene="ligA"
                     /locus_tag="PLH57_11150"
     CDS             <1..>1447
                     /gene="ligA"
                     /locus_tag="PLH57_11150"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRK03211.1"
                     /translation="NHHYYVLDKPLISDQEYDQLFRELDSLEKKYPELRTANSPTQRV
                     GAPPLDKFKKHSHALPMLSLANAYSSEEIEDFDLRVKKFLNMPVDQTIEYWAELKFDG
                     LSMSLTYENGNLTVAATRGDGTVGENVTPNIKTIRSVPLRLNGEYPPLIEVRGEILIS
                     HADFRKLNDERASADEDLFANPRNAAAGSVRQLDSSITANRPLTGFWYSLGAADDVWR
                     RSLKTQQNILEQLRRWGFKVSSTGELCKGVAQVEKFYNEIQAKRESLPFDIDGIVVKV
                     NSVRLQDELGFVARAPRSMIAYKYPARQETSVVKDILVQVGRTGALTPVAILEPVNVG
                     GVVVSRVTLHNAQEIERKDVRIGDTVVVQRAGDVIPEIVKVVQEKRKSSSVAYAFPKK
                     CPSCGARAVIEDDEAVTRCVNTDCQAQIIETIAHFASKDAMNIDGLGFRIVENFVNRG
                     LLKDSADLYRLQKTDLEHLEGFGEKSASNIVA"
BASE COUNT          410 a          341 c          343 g          353 t
ORIGIN      
        1 caaccatcat tactatgtat tggataagcc actgatttcc gatcaggaat acgaccagct
       61 atttcgagag cttgattcat tagaaaaaaa atatcccgaa ctccgaactg ccaactctcc
      121 cactcaacgc gtgggggccc caccgctaga caaatttaag aaacattccc acgcattgcc
      181 gatgctcagt cttgcgaacg cgtattcttc tgaagaaatc gaagattttg atttgcgagt
      241 aaaaaagttt ctaaatatgc ccgtcgacca aacaatcgag tactgggccg aacttaaatt
      301 cgacggactt agtatgtcgc tcacctacga aaacggcaat ttaacggttg cagcgacccg
      361 tggtgacggc acagttgggg aaaacgttac tcccaatatt aaaacaattc gatcggttcc
      421 attacgttta aatggggaat atccgcctct catagaggta cgtggagaaa ttcttatatc
      481 tcacgctgat tttcgaaaat taaacgatga acgggcctct gctgacgaag accttttcgc
      541 caacccacgc aacgcagcgg caggaagtgt acgacagttg gactcgagca ttacagcaaa
      601 tcgaccactt acgggtttct ggtacagcct tggagcggcc gacgatgtct ggcgtcgttc
      661 gctcaaaacc caacaaaata ttctagagca actgcgacgt tggggcttta aggtttcgtc
      721 gactggcgag ctttgcaagg gtgttgccca agttgaaaag ttttataacg aaattcaagc
      781 aaaacgagag tcactcccgt tcgacatcga tggaatcgtc gtaaaagtaa attccgtccg
      841 ccttcaagat gaacttggtt ttgtggcccg cgcgcctcgc agcatgattg cttataagta
      901 ccccgcgaga caggaaactt ctgtggtgaa agacattctg gtgcaggtcg ggcggaccgg
      961 tgcattaact cctgtcgcga ttttggaacc ggttaatgtg ggcggggtcg tggtttcccg
     1021 agtgacacta cacaatgccc aagaaataga acgtaaagac gttcgcattg gcgacacggt
     1081 cgtagttcag agagccggtg atgtaattcc cgaaattgtt aaagtggtac aagaaaaacg
     1141 aaagtccagc tcggttgcct atgcatttcc caaaaaatgc ccttcctgtg gcgcaagagc
     1201 tgtaatcgaa gacgacgaag cggttacccg ctgcgtaaat accgattgtc aggcccaaat
     1261 aatcgagacg atcgcccatt ttgcatcaaa agacgctatg aacattgatg gattgggttt
     1321 cagaatcgtg gaaaactttg ttaaccgcgg cttgctcaaa gattccgccg atttgtatcg
     1381 attacagaaa accgacttag aacatttaga gggtttcggc gaaaaatccg cctctaatat
     1441 tgtcgct
//
LOCUS       DAOMXO010000642         1445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_52821_length_1445_cov_4.392086, whole genome shotgun sequence.
ACCESSION   DAOMXO010000642 DAOMXO010000000
VERSION     DAOMXO010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52821_length_1445_cov_4.392086"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..538)
                     /locus_tag="PLH57_11155"
     CDS             complement(68..538)
                     /locus_tag="PLH57_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRK03212.1"
                     /translation="MAFTPSSSQGGSDLDGTGSSDGIVAEINITPLTDVFLVLLIIFM
                     VTSSVLTQMGVNVQLPQSKQASEAAEPGGVIVTVTGQEQIMVNGASVARTHIEDALRL
                     ALSRSKDKTIVLEGDRRAVLGTVVEIMDAGKKAGASKFAVATAPEGGPSKAPER"
     gene            complement(550..1161)
                     /locus_tag="PLH57_11160"
     CDS             complement(550..1161)
                     /locus_tag="PLH57_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRK03213.1"
                     /translation="MSIFELIKTGGWVMVPLLICSVVVWAIVVERALALRRWSEQNRE
                     FLLSFTNAWLKGDREAAQKLCQTRDLDIAQVAREFLSDKVPARDLVARAERKRLERAQ
                     ELRRFLWILGTIGSASPFIGLFGTVVGIIKSFQAMAESGTGGFAVVAAGISEALVATA
                     AGIFVAVIALFFYNMFQVRIGKLQFQLKLLTEELTENVTSRSV"
     gene            1217..>1445
                     /locus_tag="PLH57_11165"
     CDS             1217..>1445
                     /locus_tag="PLH57_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03214.1"
                     /translation="MGNQARFFIFSLFTLLCGTLSYAAPPAAWSWIEVRLGKDSLRAT
                     IQNPEAFKPDWRLTHWNKARVFDERLHSNMVN"
BASE COUNT          365 a          417 c          326 g          337 t
ORIGIN      
        1 cagcgcgctt acgaggagct tggctttaag atcgaacaat cactgcgcgc cggcgaatgg
       61 atatcgatca tcgctcgggc gccttagagg gaccgccttc gggcgccgtc gccacggcaa
      121 acttactggc cccagccttt ttaccagcat ccattatttc gacaacagtt ccgagtaccg
      181 ctcgtcgatc gccttcgagt acaatcgttt tatctttaga acggctgagc gccaaacgca
      241 gcgcatcttc gatgtgagtt cgggccaccg atgcgccgtt gaccatgatc tgctcctgtc
      301 ctgtaactgt aactatgacc ccacctggtt ccgccgcttc tgatgcttgt ttgctctgtg
      361 gcaattgcac gttcacaccc atctgcgtga gaacgctact cgtcaccatg aaaataatca
      421 gcagtacaag aaacacgtcg gtgagcggag tgatgttaat ttcagcaaca ataccgtcgc
      481 ttgaaccggt tccgtcgaga tccgaaccac cttggctgct gcttggggtg aatgccataa
      541 ttgtaatctc taaacagaac gggaagtaac gttctcagtg agctcttccg taaggagttt
      601 caattgaaac tgaagtttgc caatccgtac ctgaaacata ttgtaaaaaa ataatgcgat
      661 tacagcgaca aatattccag ctgccgtagc aacaagggct tcagaaattc ctgctgccac
      721 cactgcgaaa ccaccggttc cactttctgc catcgcctga aacgatttga taatacccac
      781 gaccgttccg aacaatccga taaacggcga agctgagcca atggtgccca gaatccacag
      841 aaagcgtcgc agttcctgag cccgttccaa acgcttgcgc tctgcccgcg cgaccaggtc
      901 tctagccggt actttatctg ataaaaactc acgtgcgacc tgcgcgatat ctaagtcgcg
      961 ggtctggcag agtttttgcg ccgcttcccg atcgcccttt agccaggcat tggtaaacga
     1021 caataaaaac tcccgattct gttcactcca acgccgcaat gccaacgcgc gctcgaccac
     1081 gatcgcccaa actacgaccg agcaaattag cagcggcacc atgacccaac caccggtttt
     1141 aatgagttcg aaaatcgaca tattttacct caactgtgct tgaaaaagct aggaaaccaa
     1201 acttactctg tcaatcatgg ggaaccaagc acgattcttt attttcagtc tttttacact
     1261 cttatgcgga acactcagct acgctgcccc gccggcggcc tggagctgga tcgaggttcg
     1321 tcttggcaag gactctctcc gagcaacgat tcaaaacccg gaggccttta aacccgactg
     1381 gcgcctgact cactggaata aggccagagt gttcgacgaa cgccttcatt ccaatatggt
     1441 gaata
//
LOCUS       DAOMXO010000643         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_52929_length_1443_cov_4.117435, whole genome shotgun sequence.
ACCESSION   DAOMXO010000643 DAOMXO010000000
VERSION     DAOMXO010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52929_length_1443_cov_4.117435"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLH57_11170"
     CDS             complement(<1..497)
                     /locus_tag="PLH57_11170"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRK03215.1"
                     /translation="MSKIKLPAPLRHAPRQTLERVSAVFFDIDDTFSLHGKILSESYD
                     ALWRLRKAGFRLVPVTGRPAGWCDMIARMWPIDGVVGENGAFYCSLDEKKKKLEKIYL
                     ETPKVRAANQKKLIALKKKLIKKFPGLRTPSDQPYREFDLAIDYCEDVKRWPEKRVQQ
                     WLEFCR"
     gene            complement(490..>1443)
                     /locus_tag="PLH57_11175"
     CDS             complement(490..>1443)
                     /locus_tag="PLH57_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRK03216.1"
                     /translation="GSLEESILKRKNASTLALAATFLLTNVKMVVAVFTGSVGVLSEA
                     IHSGLDLLSSLVTFFVVRASGKPADLNHPFGHGKIESISALFEAFLLFVAGGYIVFEG
                     VRAWANSDSHHIHNTGWGIAALGGSIVVNLFVYLQNRKVAREHESIAIETNAFHFLTD
                     MFSSLAVFVSLIIVQYTGWVWVDALVAVVIAAYITVVGIQQMRKCIAELSDTALPDDE
                     VGAIAKAIEKHKSKFVNYHDLRTRKSGAKRHVDFHLEICSEDRVARAHAVCDEIEDDL
                     MKQFKETDVNIHVEPCGHHEAACEKVCLFYRKDRSEVARSV"
BASE COUNT          368 a          357 c          343 g          375 t
ORIGIN      
        1 cgacaaaatt cgagccactg ttgcactctt ttctcgggcc accgcttaac gtcctcgcag
       61 tagtcgatgg ccaagtcgaa ttcgcgatac ggttgatcac tcggcgttcg aagaccggga
      121 aattttttta tcaatttctt tttaagtgca attaactttt tctgatttgc ggctcgtact
      181 ttcggagttt cgagatatat tttctcaagt tttttctttt tttcatcgag cgagcaatag
      241 aacgcaccgt tctcgcccac tacgccgtca attggccaca tgcgcgcgat catgtcgcac
      301 cagccggcgg ggcgtccggt cacgggcacc aaacgaaagc cggctttacg caaccgccag
      361 agcgcatcgt aggattcgct caaaattttt ccgtgtaacg aaaaggtatc gtcaatatcg
      421 aagaaaacag cactcactcg ttcgagcgtt tggcggggcg catgcctcag aggtgcaggc
      481 aacttgattt tagacactcc gtgcaacctc agaacggtct ttgcggtaga acaagcaaac
      541 cttttcacag gccgcttcgt ggtgcccaca aggttcaaca tgaatgttta catcggtttc
      601 tttaaattgt ttcatcagat cgtcttcgat ctcgtcacaa actgcgtgcg ctcgtgccac
      661 ccgatcttcg gaacatattt cgaggtgaaa atcgacgtgg cgtttggcgc ctgattttcg
      721 cgtgcgcagg tcgtggtagt ttacgaattt ggatttatgt ttttcgatcg ctttggcgat
      781 agcccctact tcgtcgtcgg gcagcgcggt atctgatagt tcggcaatac acttacgcat
      841 ttgttgtata ccaacgacgg taatgtaggc tgcaataacc actgcaacaa gcgcgtccac
      901 ccacacccaa ccggtgtatt gcacgatgat caaacttaca aatactgcca aactggaaaa
      961 catgtcggtt aagaaatgaa acgcattcgt ttcaattgcg atgctttcgt gttcgcgggc
     1021 gactttgcgg ttttgcaaat aaacgaacag gttaaccacg atcgagccac cgagggcggc
     1081 gattccccag ccagtgttgt gaatatggtg ggaatcgcta ttggcccacg cacgaacacc
     1141 ttcgaaaaca atgtacccac cggcaacaaa taggaggaac gcttcgaaaa gggctgaaat
     1201 gctttcaatt tttccgtgac caaacgggtg attaagatca gcgggtttgc ctgaggctcg
     1261 cacgacaaaa aacgtcacaa gcgaagagag taggtctaag ccagaatgaa ttgcttctga
     1321 gagtacaccc acgctgccgg taaaaacagc cacgaccatt ttgacgttag ttagtaaaaa
     1381 ggtagcagct agagctaatg tcgacgcgtt tttacgcttt agaattgatt cttctaaaga
     1441 acc
//
LOCUS       DAOMXO010000644         1435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_53343_length_1435_cov_4.529710, whole genome shotgun sequence.
ACCESSION   DAOMXO010000644 DAOMXO010000000
VERSION     DAOMXO010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53343_length_1435_cov_4.529710"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLH57_11180"
     CDS             <1..632
                     /locus_tag="PLH57_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03217.1"
                     /translation="CSSFLPQTRMPVGVASALKVEKSSPSSLAPLLRTFALLVFLFFA
                     VAFTTTIPAEAVVRVAFLTKKNAEGHLLRLHPTGHGFYHVAVSYKGRWLHAHPNGGVQ
                     ISKSLRRFGQIAAVLEHRKLREPSTRFVRKIRGLPYDPAFRWNEPESFYCSKLLAKHF
                     RLPPTPMTFHGSHWGEYFRQLGLPKPINEVGLSPDEVYDLLIERGFVPR"
     gene            660..1109
                     /locus_tag="PLH57_11185"
     CDS             660..1109
                     /locus_tag="PLH57_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRK03218.1"
                     /translation="MFLTLIFVANCARVSASEIPDYQCLDCTVEATSEGKVLLNYDLV
                     LFRDAKKRNLFKGSLAVRFVDQNGKLVPVKDVSLTSYMPGMGHGSGKEPTAKPETIDD
                     NERAAVWISNDVIFTMAGIWRISVQFKGAGKDDEARIIAWVRKPESK"
     gene            1106..>1435
                     /locus_tag="PLH57_11190"
     CDS             1106..>1435
                     /locus_tag="PLH57_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRK03219.1"
                     /translation="MNALVKIATLFLLWALCLAATPLPNEIELSKLDGTMVKLSLNSR
                     FTAFAFLSARCPCSKSHEEKLSKLCARFSNRGIRCIGIHSNANEPLSEAAKYFGTANL
                     SFPVLHDP"
BASE COUNT          354 a          377 c          321 g          383 t
ORIGIN      
        1 gttgctcttc attcctacct cagacgcgaa tgccagtggg ggttgcttca gcgctgaaag
       61 tagagaaaag ctctccttct tcgctagcac cacttttgcg tacttttgca ctattagttt
      121 ttttattttt cgcggttgcc ttcaccacca caattccagc tgaggcggtg gttcgggtag
      181 cctttctaac caaaaaaaac gccgaaggac atttgcttcg actgcacccc acgggccacg
      241 gtttttacca cgtcgctgtt tcttacaaag ggcgttggct acacgcacac cctaacggcg
      301 gggtccaaat ttccaaatct ctccgtcgct ttggtcaaat tgcggcagtg ctcgaacacc
      361 gcaaattacg cgaacccagt actcgattcg ttcgaaaaat tcgaggactg ccctacgacc
      421 cagcgtttcg ctggaacgaa cccgaatcct tctactgttc taaattactc gccaaacact
      481 ttcgattgcc ccccaccccg atgacattcc acggctctca ctggggcgaa tattttcgcc
      541 agcttgggct acctaaaccg atcaatgagg tcggccttag tcctgacgaa gtttatgatt
      601 tattgattga acgcggcttt gtgccgagat aaccagaccc catgcatttc gttgttaaca
      661 tgtttttgac tctaattttc gtggctaatt gcgctcgcgt ttcggcctcg gaaattccag
      721 attatcagtg tcttgactgt acggtcgaag caacaagtga aggcaaagtt ctattgaact
      781 acgatcttgt gctctttcgg gacgccaaaa aaagaaacct tttcaagggc tcccttgccg
      841 ttcgattcgt tgatcaaaat ggtaaactgg taccggttaa agacgtttcc ttgacgagct
      901 acatgcccgg aatggggcac ggatccggaa aagaaccgac agccaaaccc gagaccatcg
      961 acgataacga aagagcggcg gtttggattt caaatgatgt gattttcacg atggccggaa
     1021 tttggcggat ttccgtgcag ttcaaaggcg cgggcaaaga cgacgaagct cgaattattg
     1081 cctgggttag aaagcccgag tccaagtgaa cgcgttggta aaaattgcaa ctttgtttct
     1141 tctttgggct ctatgtttgg ccgctacacc tctgccaaac gaaatcgaac tctcaaagct
     1201 ggatggaacg atggtaaagc tgtctctcaa ttcacggttc acagccttcg cgtttctttc
     1261 tgcacgctgc ccctgctcta agtcacacga agaaaaactt tccaaacttt gcgctcgatt
     1321 ttcaaaccgc ggaataaggt gcattggaat tcattccaat gcaaacgaac ctctcagcga
     1381 agccgcgaaa tactttggaa cagccaactt atcttttcca gttttgcatg acccc
//
LOCUS       DAOMXO010000645         1434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_53395_length_1434_cov_4.673677, whole genome shotgun sequence.
ACCESSION   DAOMXO010000645 DAOMXO010000000
VERSION     DAOMXO010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53395_length_1434_cov_4.673677"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLH57_11195"
     CDS             <1..422
                     /locus_tag="PLH57_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRK03220.1"
                     /translation="IGIAVDTEDGLIVPNIKNADQKSILQLAAEITELAEKARTRRLS
                     LEEMQGGTFTITNPGPIGGLITAPVINWPEVAILAVHKIVKRPIVTEDDEIKAASMMY
                     LSLAVDHRVVDGAVAARFMNVVIEYLASPKLMMLDMA"
     gene            437..>1434
                     /locus_tag="PLH57_11200"
     CDS             437..>1434
                     /locus_tag="PLH57_11200"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK03221.1"
                     /translation="MATKQYDAVVIGGGPGGYVCAIKLGQLGVKTALVEREFLGGVCL
                     NVGCIPSKALIHAGTFYEQIQHHAPELGIDVQVKGFDLTKLVAWKNGVVKKLTGGVGG
                     LLKANKVDVINGEAKFENPTTLTVKTAQGTETLTAKKFVIATGSRPFVIPGFEPDGKS
                     ILTSTEALDLTSAPKNLVVIGGGYIGLELGTYLAKVGSKVTVLEGTKQLLGSMDQDCV
                     GVVARALKKKGVQVLTEAKAKSYKKNGKKLEVTVELPTGTQKIECDAVLVTVGRRPNS
                     DTLALEKAGLKPDSKGFITIDKTCRTAIPHIYAIGDVAGGLLLAHKASKEGIVAAE"
BASE COUNT          420 a          315 c          370 g          329 t
ORIGIN      
        1 acattggtat cgcagtcgac accgaagacg gcttgattgt tccgaatatt aaaaacgccg
       61 atcaaaaaag tattttacag cttgcagccg aaatcactga acttgctgaa aaagcgcgta
      121 ctcgcagact ctcgctcgaa gaaatgcagg gcggaacgtt taccatcacg aatccgggac
      181 cgatcggtgg attgatcaca gcgcccgtga tcaactggcc agaagtcgcg atcctagcgg
      241 tgcacaaaat tgttaaacgc ccgatcgtga ccgaggacga cgaaataaaa gcagcgagta
      301 tgatgtatct atccctcgcg gtagatcatc gcgtagttga tggcgccgtg gcggcgcgct
      361 ttatgaacgt ggtcattgag tacctagcat cgcctaaact catgatgttg gacatggcgt
      421 aaggagattc acttttatgg caaccaaaca atatgatgca gtggtgattg gcggcggtcc
      481 agggggctac gtttgcgcta ttaaactagg acagctcggc gtaaaaacag ccttggttga
      541 gagggaattt cttggtggcg tttgtttaaa tgtcgggtgc attccttcaa aagcgctcat
      601 tcacgcgggt actttttatg aacagatcca acaccacgca ccagaacttg gaattgatgt
      661 tcaagtaaaa gggtttgatc ttaccaaact tgtcgcctgg aaaaacggcg tagtgaaaaa
      721 actcaccggc ggtgtaggcg gattattgaa agccaataaa gtagatgtca ttaacggcga
      781 agctaaattt gaaaatccca ctacgctcac agtcaaaact gctcagggca ctgaaacact
      841 gactgcaaaa aaattcgtga ttgcaactgg atcgcggcca tttgtgattc caggttttga
      901 acccgatgga aaatcaattc tcacttctac cgaagcgtta gacctgacga gcgctccgaa
      961 aaacttggtc gttattggtg gcggttacat tgggctcgag ctgggcactt atctagctaa
     1021 agttggttct aaagtgacag tgttagaagg aacaaaacag ttgcttggat ctatggatca
     1081 agactgcgtt ggagtcgtcg cacgcgcgct taagaaaaag ggagtgcagg tacttaccga
     1141 agccaaggcc aagagttaca aaaaaaatgg caagaaactg gaagtcacag ttgagctacc
     1201 aactggtact caaaaaatcg aatgcgatgc ggttttggta acagtaggtc gtcgcccgaa
     1261 ctctgacacg cttgcactcg aaaaagcagg gctcaagcca gattccaagg ggttcattac
     1321 gatcgacaaa acttgtcgca cagcaattcc gcatatttac gccataggcg acgtagctgg
     1381 tggattgctt ctcgctcaca aggcgagcaa agaaggaata gtggcggcag aaaa
//
LOCUS       DAOMXO010000646         1433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_53480_length_1433_cov_3.049347, whole genome shotgun sequence.
ACCESSION   DAOMXO010000646 DAOMXO010000000
VERSION     DAOMXO010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53480_length_1433_cov_3.049347"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLH57_11205"
     CDS             <1..539
                     /locus_tag="PLH57_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRK03222.1"
                     /translation="RLGLPYREVLIKNRYIKRTFILNSQKNREEAVDLKLNPVVSEIK
                     GKTVILVDDSIVRGTTSRKIIQLVRRAGAKKVYFVSTCPPIRFPCYYGFDFPEQNELI
                     AAGPNGSRTEEEITKMLDADGVFYQTLDSLGNALSKCSLGLVKNPCTACLTGKYPTDM
                     KGAAELTALRRTDRGKSE"
     gene            536..>1433
                     /locus_tag="PLH57_11210"
     CDS             536..>1433
                     /locus_tag="PLH57_11210"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRK03223.1"
                     /translation="MSARALVAEGSVKNLWSTENSNEIDFEFSDAYSVFDWGRMPDQL
                     QGKGEALAQIGAFFFETLASPDPWTDFFKNADAAVVRLGTKQGLSGETLIKTADELTR
                     KGLISHYLAAVDTRSFRVRRAEVFVPKPATYYGSSAFSYPLQPYLLCRSLKLIPLEVV
                     FRFGVPHGSSYFARATQSSVESLGATWPLKEQARFSFPVIEFFTKLEDTDRFLNFTDA
                     MTLSGLSKVAFDRLIAKTALLGLWLQNFFATRGLELWDGKFEWGALETNDGTELVLLD
                     SIGPDELRLTEPKSGMQVSKEFL"
BASE COUNT          344 a          330 c          372 g          387 t
ORIGIN      
        1 agcgattggg tcttccttac cgagaagttt taataaaaaa ccgctacata aaacgcactt
       61 ttattttgaa ttctcaaaaa aaccgagagg aagcggtgga tctaaagttg aatcctgtgg
      121 tgagtgagat caagggtaaa acagtgattc tagtcgacga ttcgatcgtg cgaggcacga
      181 cttcgcgcaa gatcattcaa ctcgttcggc gggccggtgc taaaaaagtc tattttgtga
      241 gcacttgtcc acccattcga tttccttgtt actatggctt tgattttcca gaacagaacg
      301 agctcatcgc tgccgggccg aatggttccc gcactgaaga ggaaattacc aagatgctcg
      361 acgccgatgg tgtgttttat caaacgcttg attcgctggg caatgcgctt tcgaaatgtt
      421 ctttgggtct ggtgaaaaac ccctgtaccg cgtgtttgac tggtaagtat cctaccgaca
      481 tgaaaggggc ggccgaatta acagccctgc ggcgcaccga ccggggtaaa agtgaatgag
      541 cgcgcgggct ttggtcgctg aaggcagcgt taaaaattta tggagcaccg agaattcaaa
      601 cgaaattgat tttgaatttt ccgatgccta ttcggtgttc gactgggggc gaatgcccga
      661 ccaattgcag ggcaaaggcg aggcactcgc gcagattggg gccttttttt tcgaaacctt
      721 ggccagtccg gatccgtgga ctgatttctt taaaaatgct gacgcagcgg tcgtacggct
      781 tggcactaaa caaggacttt ccggtgagac actcattaag actgccgatg aactcacgcg
      841 caagggatta atttcgcatt acctcgcagc ggtggatacc agatcttttc gcgttcggcg
      901 tgcggaagtg ttcgttccga aacccgctac ttattatgga tcttctgcgt tttcgtatcc
      961 ccttcagccc tatctgttgt gtcgcagcct aaagctgatc ccgcttgaag tggtatttcg
     1021 tttcggtgtg ccgcacggtt cttcctactt tgctcgagcc acccaatcga gcgtcgagag
     1081 tcttggcgca acctggccgt tgaaagaaca ggccaggttt tcgtttccgg ttatcgaatt
     1141 ttttaccaag ctcgaagata ccgatcgatt tttgaacttt actgacgcga tgacacttag
     1201 tgggcttagt aaggtggcct ttgatcgttt gatagccaaa acggcgctac ttggtttgtg
     1261 gcttcaaaat tttttcgcta caagagggct cgagttatgg gacggaaaat ttgagtgggg
     1321 cgcgctagaa accaacgacg gcacagaact cgttttgctt gattctatcg gtcccgacga
     1381 attacgcctc acggaaccaa aatctggaat gcaggtgtct aaagaatttc tgc
//
LOCUS       DAOMXO010000647         1429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_53660_length_1429_cov_5.040757, whole genome shotgun sequence.
ACCESSION   DAOMXO010000647 DAOMXO010000000
VERSION     DAOMXO010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53660_length_1429_cov_5.040757"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..>1429)
                     /gene="murJ"
                     /locus_tag="PLH57_11215"
     CDS             complement(22..>1429)
                     /gene="murJ"
                     /locus_tag="PLH57_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRK03224.1"
                     /translation="NTTKAAFRLLSSVVAVLVVGLSALSFVGICLSEPLVRLYASAFA
                     SVPGKLELATQLTQVMFPFFPMVALAAVLMGALNAMGSFFLPAFAPVLFNVASILCGV
                     ILTPLFANHTSLPPIYSMAVGVLVGGFLQFYVQWWQLRKKGYRPLEFAKNFARPFADP
                     GTKKVLLLLVPGTVGLAATQLNILVNSVYATHFGSGAVSWLNYAFRFLQFPIGIFGVS
                     LAVATLPLVTRKLAKGSPKEAADEVANSLHLVFAVNGPAAAGLMVVGLPIVQMIFQHG
                     QFKLFDAQQTAQALFWYALGLPGYSVVKVLVPVFYALERTRIPVTISFVMVGLNLVLN
                     HLFSAVWGFPFWALALSTSIVATLNAVLLLYFLNRKMGGILGTQFFSRLFAHDAVAAA
                     VGATSYAASSGAATFWVKIGINTGVFTWSSQVVCALLAAIATWLAVGKVFRISEIDRA
                     LNVVVRRFRGKNGNSDHQ"
BASE COUNT          432 a          382 c          332 g          283 t
ORIGIN      
        1 gccaaaactt taagtgagtg cttattggtg gtctgagttt ccatttttcc ccctaaaacg
       61 ccgaacgacc acgttcaaag cgcgatcgat ttctgaaatc ctaaacacct tgccaacggc
      121 aagccaggtt gcgatggccg ctaatagagc acaaacaacc tgcgaactcc aagtaaaaac
      181 ccccgtattt attccgattt ttacccaaaa agtggccgcg ccgctactgg cggcataact
      241 cgtggcaccg accgcggctg ccacagcgtc atgtgcgaaa aggcgcgaaa aaaattgggt
      301 accaagtatt ccacccattt ttctatttaa aaaatacagc agtaatacgg cgttgagcgt
      361 ggcgacgatc gaagttgaga gcgccaacgc ccagaacgga aacccccaga ctgctgaaaa
      421 taaatggttg agcactaggt tcaaacctac catgacaaag ctaatcgtaa ccggtatgcg
      481 cgtgcgttcg agcgcgtaaa acacaggcac aagaactttc acaactgagt accctggcag
      541 tcctaatgca taccaaaaaa gtgcctgcgc ggtttgttga gcgtcaaaaa gtttaaattg
      601 accgtgttga aagatcattt gcacgatcgg gagtcccacc accattaatc cagccgccgc
      661 cggaccattt accgcaaata ctaaatgcaa cgaattcgcc acttcgtctg ccgcttcttt
      721 gggtgagccc ttagcaagtt ttcgcgtgac tagaggcagg gttgccaccg ccaaagaaac
      781 tccaaatatg ccgataggaa actgcaaaaa cctaaacgcg taattcaacc agctcaccgc
      841 gccagagcca aaatgggtgg cataaaccga gttgaccaat atattaagtt gggtcgccgc
      901 tagccccacg gtaccgggga ccaaaagcag aagcactttc ttagttcctg ggtcggcaaa
      961 gggtcgagca aagttctttg caaattccag gggtcggtac ccttttttcc gaagttgcca
     1021 ccactgcaca taaaattgga gaaaaccacc gaccaaaact ccgaccgcca tgctgtagat
     1081 tggcggtaaa gaagtatgat tcgcaaacaa tggagtgaga ataaccccgc acaaaatcga
     1141 cgccacattg aacaatacgg gggcaaacgc tggcaaaaag aacgaaccca tggcgtttag
     1201 ggcgcccata agtaccgcag cgagcgcgac catggggaag aatggaaaca tgacttgggt
     1261 gagttgcgtt gcgagttcta gtttgcctgg cacactcgca aatgcgctag catacaaacg
     1321 aacgagcggt tcgctcaaac aaataccgac aaacgacaac gcactcaacc ctactaccaa
     1381 aacagccacg accgaagata gcaaccgaaa cgccgccttg gtggtattc
//
LOCUS       DAOMXO010000648         1429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_53669_length_1429_cov_4.140466, whole genome shotgun sequence.
ACCESSION   DAOMXO010000648 DAOMXO010000000
VERSION     DAOMXO010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53669_length_1429_cov_4.140466"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLH57_11220"
     CDS             <1..682
                     /locus_tag="PLH57_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRK03225.1"
                     /translation="RCPLTRTLLGTTVRGLRLWKPLAEISQELQDFVILLEDAKFYDH
                     TGLDLEEIRNAIEEDVQDRKFSRGASTITQQLAKNLFLTKDKSMVRKATEIPLALRIE
                     KELTKKQILELYLNTIEWGPELFGAEAAARTYFGHTAKEISTHEAILLALIIPNPKYL
                     NPWVRPAALENLEQRATQFKKRLVREKKMTPEAARDVFDSCPAFLREWSAREKPYTPA
                     TKPKRQTR"
     gene            743..1381
                     /gene="rdgB"
                     /locus_tag="PLH57_11225"
     CDS             743..1381
                     /gene="rdgB"
                     /locus_tag="PLH57_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRK03226.1"
                     /translation="MRPTNMIMLASQNAGKIREYQSLFNKYPEYKLVTVGEITSNASA
                     LAQAENGKTYYENAFEKGQLAHLAGKLPTISDDSGLEVDALGGRPGVLSDRYASIEPG
                     YSKSESNVRKLLEELRGVPKDKRTARFVCTLVFFMEGVVVSATGTVEGSILETPRGPN
                     GFGYDPIFLVKDTDLSMAEMALEQKNELSHRARALAALMQQIKEKNLKLVRP"
BASE COUNT          413 a          363 c          326 g          327 t
ORIGIN      
        1 ccgctgtcct ttgactcgta ctttgttggg gacaaccgtg cggggccttc gactttggaa
       61 gccactcgcg gaaatatcgc aagaacttca ggattttgtg attcttttag aagatgcgaa
      121 attttatgac cacactggtt tagatctcga agagattcgc aacgccattg aagaagacgt
      181 tcaggatcgt aaattttcgc gcggcgccag cacgattact caacagttgg ccaaaaatct
      241 ttttctcacc aaagacaaat cgatggtccg caaggccaca gaaattcctc tagcgctacg
      301 aattgaaaaa gaactcacaa aaaaacagat tttggaattg tatctcaata cgatcgaatg
      361 gggccccgaa ctttttgggg ccgaagctgc tgctcgcacc tatttcggcc acaccgccaa
      421 ggagataagt acccatgaag caatcctcct ggctcttata attcccaatc cgaagtacct
      481 aaacccgtgg gtgcgacctg cggccctgga aaatctggaa caacgagcca cgcaatttaa
      541 aaaacgatta gtacgagaaa agaaaatgac accggaagca gcgcgcgatg ttttcgactc
      601 atgtcccgcc tttcttcgcg agtggagcgc ccgggaaaag ccctacaccc ccgccacaaa
      661 acctaaacga caaacgcgct agaaaatcct ctccagtggc gttatgttgc tggctaccac
      721 aaccaagcag gagaacgcct ctatgagacc taccaacatg atcatgctgg cctcgcaaaa
      781 cgccggaaaa attcgcgaat accaatcgct atttaataaa tatcctgaat acaaattggt
      841 tactgtcggc gaaatcacgt cgaacgcatc ggccttggcc caggccgaaa acggtaaaac
      901 ctattatgaa aatgcgtttg aaaaaggaca acttgcgcac ctagcaggaa aactccctac
      961 catcagtgac gactccggct tggaagtcga cgcactcggt ggacggccag gcgttttatc
     1021 agatcgttat gcttcaatcg aaccgggcta cagcaaatca gaatcgaatg tacgtaaact
     1081 tcttgaagaa ctgcgaggag taccgaaaga caaacgcacc gcgcgattcg tctgtacctt
     1141 ggtgtttttt atggaaggcg ttgtggtttc cgctaccgga acagttgaag ggtctattct
     1201 tgaaactcct cggggcccca acggctttgg atatgatccc atttttttgg taaaggacac
     1261 cgatttgtcg atggccgaaa tggctctcga acaaaaaaac gaacttagtc accgggccag
     1321 agctcttgcc gctctgatgc aacagatcaa agaaaaaaac ctaaagctcg tacggcctta
     1381 gtgatgaacc cttcggttaa aaccctatta ggggttttgc tcttagttg
//
LOCUS       DAOMXO010000649         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_53834_length_1426_cov_5.466083, whole genome shotgun sequence.
ACCESSION   DAOMXO010000649 DAOMXO010000000
VERSION     DAOMXO010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53834_length_1426_cov_5.466083"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..864
                     /locus_tag="PLH57_11230"
     CDS             19..864
                     /locus_tag="PLH57_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03227.1"
                     /translation="MCSTKTLITAKGGRAFVYFERLSLGTRTSIVRFFAMLLFALALN
                     CVYDVLCYAQSGPPVPSMNRGFSGGSSRGSGSRGGRAGSMGFRFGPSYLSLAHKQSNA
                     SAVTYSALAPELNIILPLSRSTFLEGGGYYSALEIGNSTSADITYWSGFGRLIFPFIS
                     GRKNNWYLGPGVFYRTMTVSTNNFGYQDISGPDISTYLEFNLKRKQRIQIGGRYGLVY
                     TGEIAVGASRELNGFLKIALPVKKNFFTFQFDYTKTTAELTTTTRTTVTVNSTVMRFS
                     ILFGK"
     gene            complement(888..>1426)
                     /locus_tag="PLH57_11235"
     CDS             complement(888..>1426)
                     /locus_tag="PLH57_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03228.1"
                     /translation="NAWSLGLPLFSGATSGYVVPAYGFLLGFSTAALTSKTFNIVNAF
                     DVGLGVGGTQFGSVGSAYNLIFGMYDLGLRINLMKKMLQPYFSFGPTLGFFAANLTSP
                     PATQSTNQASLKYGYWLAAGFDWYKDDAGRGSSSAWGLELTYFNYLASPGLFEFPAGR
                     LGVRGIKFEVHFILAPSK"
BASE COUNT          381 a          353 c          333 g          359 t
ORIGIN      
        1 tacaactaaa gggatgtggt gtgttctacg aaaaccctta tcaccgcaaa ggggggccgc
       61 gcgttcgtgt attttgaacg actctctcta ggcactcgta cgtcgatcgt acgttttttc
      121 gcgatgttgt tattcgcgtt ggcgctcaat tgcgtctacg atgtgctttg ctacgcgcaa
      181 tcgggcccac ccgttccatc aatgaatcgc gggttcagtg gtggttcgtc gcgcggcagt
      241 ggtagtcgtg gcggaagagc aggctcaatg ggttttcgtt ttggcccaag ttacttgtca
      301 ctcgcgcaca aacaatcgaa cgcatcggcc gtcacgtatt ctgcgctggc gccggaactc
      361 aacataatat taccgctcag ccgatctacc ttcctcgagg gcgggggcta ctactccgca
      421 ttagaaatcg gcaacagtac ttcggccgac attacttatt ggagtggttt cggacgatta
      481 atttttccat tcatttcggg aagaaaaaat aattggtacc tcgggcccgg ggtgttctat
      541 cggactatga cagtttctac aaataatttt ggttatcaag atatctcagg acccgacatt
      601 agtacttacc ttgagtttaa tctcaaaaga aaacaacgca ttcaaatagg tgggcgatac
      661 ggcttagttt acactggaga aatagcggta ggtgccagcc gcgaactaaa cggattttta
      721 aaaatcgcgc tacctgtgaa gaaaaacttc tttacctttc agttcgacta caccaaaacc
      781 accgctgaac tcacaacaac caccagaacg accgtcaccg tgaattcgac agtgatgaga
      841 tttagtattc tatttggcaa gtaacagcat tggcttattt gtagtggcta cttagacggt
      901 gcgagaatga aatgcacttc gaacttaatg ccgcgcacac cgagcctgcc ggccggaaat
      961 tcaaacagtc caggcgacgc aaggtagtta aaataagtta gctctagacc ccaagcacta
     1021 gaactacctc ttccggcatc atctttatac cagtcaaagc cggccgctaa ccaataaccg
     1081 tatttcaagg acgcctgatt ggtcgactga gttgctggtg gacttgtgag attggccgca
     1141 aaaaaaccca gtgtgggccc aaacgaaaaa taaggttgca gcattttttt catcaaatta
     1201 attcgaagac ccaggtcata catcccaaat attaaattgt aggcgcttcc cacgctgcca
     1261 aattgagtgc cacccacccc aaggcctacg tcaaaggcat ttacgatatt gaatgttttc
     1321 gatgtgagag ctgcagttga aaatccgagt agaaacccat aggccggcac tacatagcca
     1381 ctggtcgcgc cgctgaaaag cggcagccct agactccagg cgtttt
//
LOCUS       DAOMXO010000650         1421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_54116_length_1421_cov_4.189605, whole genome shotgun sequence.
ACCESSION   DAOMXO010000650 DAOMXO010000000
VERSION     DAOMXO010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54116_length_1421_cov_4.189605"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..606
                     /locus_tag="PLH57_11240"
     CDS             40..606
                     /locus_tag="PLH57_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRK03229.1"
                     /translation="MNTPNISLEFTPNPNTLKYVLEETILEKGPANFATRESATKSPL
                     ATRLFEVLGVAGVMIGRNFVTITKGSDGDWESLNRGVMDVMKDHLGKGLPVVDADYVA
                     AHDAAASASAGGGIEDKIREILDREIRPAVAMDGGDITFDRFEAGVVYLQMQGSCSGC
                     PSSTATLKQGIETRLKSAIPEIQEVVSV"
     gene            complement(603..917)
                     /locus_tag="PLH57_11245"
     CDS             complement(603..917)
                     /locus_tag="PLH57_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03230.1"
                     /translation="MNSDIASAPVLALTEENFSKGFLVDDEWIAGVTELPSTTGGTVF
                     AAYVSHYLTGETIQYQEFPEPRQAIDFIRAIDRAWVYEAIGCDTHGSSAKSCSKSCSG
                     CG"
     gene            complement(914..>1421)
                     /locus_tag="PLH57_11250"
     CDS             complement(914..>1421)
                     /locus_tag="PLH57_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRK03231.1"
                     /translation="IVQLDGHADLRNTLIDDKYSHACIMRRNAEIIGPKNILSVGIRA
                     VSLEEKNYIQENGVNTIPGNFALHENLLPRFSELLIRLPHDIFLTVDLDGLDPTIMPH
                     VGTPVPGGLSWHQTVQLIKELFRRKNVIAADVVEIASNSNSQRSDFTAALLAQKIVAF
                     WARNRRLT"
BASE COUNT          352 a          332 c          388 g          349 t
ORIGIN      
        1 tatcatcggc attagaatca tctatcagga gctgaactca tgaacacgcc gaacatttcg
       61 ttggaattca cgcccaatcc gaacacgctc aagtacgtgt tagaagaaac gattttggaa
      121 aaagggcctg ctaattttgc gactcgtgag tccgccacga agtctccact cgccacccga
      181 cttttcgaag tgcttggagt ggcgggggta atgatcggcc gcaattttgt gacaatcact
      241 aaaggcagtg acggcgactg ggagagtctt aatcgaggag tcatggacgt catgaaagac
      301 cacttgggta agggtttgcc agtggttgac gccgactatg tagcggccca tgatgctgcc
      361 gctagcgcga gcgccggtgg cggaatcgaa gacaagattc gtgaaattct agaccgcgaa
      421 attcgtccag ccgtagcaat ggatggcggc gatatcactt ttgatcgctt cgaggcgggc
      481 gtggtgtatt tgcaaatgca gggctcatgc agtggttgtc cgagttcgac tgccacacta
      541 aaacaaggca ttgagacacg cctcaaaagc gctatcccag aaattcaaga agtagtgtcg
      601 gtttaaccgc agccggaaca agatttagag caagatttcg cgctactgcc gtgagtgtcg
      661 cacccgattg cttcgtacac ccatgcgcgg tcgatcgcgc gtataaaatc tatggcctgg
      721 cgtggttcag ggaattcctg gtattggata gtctcaccag tcaggtagtg actaacatag
      781 gcagcgaaaa ccgttccccc cgtagtgctt ggtaactcgg tgactcccgc gatccattca
      841 tcgtcgacta aaaaaccctt tgagaaattt tcttccgtaa gtgcgagtac gggggcactg
      901 gcaatatctg agttcatgtg agacgccgat tgcgggccca aaacgccacg atcttttgtg
      961 caagcagagc tgctgtaaag tcggaccgct gagagttgga gttgctcgcg atttccacga
     1021 cgtcagccgc gattacgttt ttgcggcgaa aaagttcttt gatcaactgg accgtttggt
     1081 gccagcttag accgccaggc acgggcgtgc ccacgtgagg catgatcgtt gggtcaagac
     1141 cgtcaaggtc cacggttaga aaaatgtcgt gggggagtcg tatgagtagt tcgctaaaac
     1201 gcggaagtaa attttcgtgt aaagcgaagt ttccgggaat ggtgttcacg ccattttctt
     1261 gaatgtaatt tttttcttct aaacttaccg cccgaatgcc aacggagaga atgtttttgg
     1321 gtccaataat ctcagcatta cgacgcatga tgcaagcgtg actgtatttg tcgtctataa
     1381 gcgtgttgcg gagatcagca tgtccatcca attgaacgat t
//
LOCUS       DAOMXO010000651         1417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_54348_length_1417_cov_3.917768, whole genome shotgun sequence.
ACCESSION   DAOMXO010000651 DAOMXO010000000
VERSION     DAOMXO010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54348_length_1417_cov_3.917768"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PLH57_11255"
     CDS             <1..1312
                     /locus_tag="PLH57_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03232.1"
                     /translation="GKPHQVFLKQHRRYLNRTGQSVLTANLFPEKKYRRIFAPYQEHR
                     LGGKRVRVVGISLETFFQETAYSQTGARIFKTEAPRKYLDTMKEQSVLAANDGVESVV
                     FLIHDGAARLTPIVEEYLNWKRSDDRVQKLLVPLVASGHMHQVIDSELHGTRVVEAGS
                     DLAFSEIQLDDNGKVSLVHHWDRRSSDQWLQQPTEPLLPEAFTELYKEANAIVDSVNA
                     ENVEILAPPLWEDRGFVLNREEHRRMRVGRVLADTLRNWALKFYSEKEVGRKISDVVG
                     LYNYLSYGWGSKIDALFEGPVTVGNLKSVVHFRGRLGARLLKGADFLELVAAMRDYGA
                     QSSRPFPNPVLSSNVVERDRRFLITDLNGTVRVVDPNGFYLVVTDHYTGMNGYKIPEI
                     DGIYQRAEWTLNDAPVNTEVLLPGLARLRLSEDSCAGVLSNFRK"
BASE COUNT          346 a          337 c          388 g          346 t
ORIGIN      
        1 cggaaaaccc catcaagttt ttttgaagca gcaccggcgt tatttgaatc gcacgggaca
       61 aagtgtgctg acggccaatt tgttcccgga gaagaagtac cgtagaatct ttgctccgta
      121 ccaagaacac cgacttgggg gaaaacgggt acgggtcgta ggtatttcac ttgagacttt
      181 ttttcaggaa actgcttata gccaaacggg cgctcgtatt ttcaaaactg aggctccacg
      241 aaaatacttg gatacgatga aagaacagag cgtattggct gccaacgacg gagtcgaaag
      301 cgtagttttt ttgattcacg atggggcagc acgtctgact ccgatcgtcg aagagtattt
      361 aaactggaaa cgcagtgacg atcgcgtaca aaagttgctg gtgccgctcg tagctagcgg
      421 ccatatgcac caggtaattg attctgaact tcatggtacg cgcgtggtag aggcggggtc
      481 ggacctagct ttttccgaga ttcagctgga cgacaacggc aaagtatcac tggttcatca
      541 ttgggaccgt cgcagcagtg atcagtggtt gcagcagcca acggagcccc ttttgcccga
      601 agcgtttacc gaactttata aagaagccaa cgcgattgta gacagcgtca acgccgaaaa
      661 tgtcgagatt ctggctccgc cgctttggga agatcgtggg ttcgttctaa atcgcgaaga
      721 gcaccgtcga atgcgagtcg ggcgggtttt ggccgatact ctaaggaatt gggcgctaaa
      781 gttttactcc gaaaaagagg tgggccgaaa aatctcggac gttgtagggc tttacaatta
      841 tttgtcctac ggatggggtt caaaaatcga cgcgcttttc gaaggtccgg tcacggtcgg
      901 gaatttaaag agcgtggtgc actttcgggg ccgcttaggc gcacgccttt taaaaggcgc
      961 ggactttctt gaattggtag cggccatgcg cgactacggc gcgcaatctt cccgtccatt
     1021 tcccaatcca gtgttgtctt ctaacgtagt cgaacgggat cgtcgtttct tgatcaccga
     1081 cctcaatggc actgtacgtg tggtggatcc aaatggattt tacttggtgg tgacggatca
     1141 ttacaccggc atgaatggct ataaaattcc ggaaattgac ggcatttatc agcgagccga
     1201 atggactctc aacgatgctc cagtaaacac tgaagttctc ctaccgggcc tcgctcgcct
     1261 gcgattgagt gaagactcgt gtgctggggt gctcagtaat tttcgaaaat aaaaaaaccc
     1321 ggggagcttt cgccacccgg gtttttcgaa gctatattct atagccgagg ttagtatcga
     1381 ccgcggccac cgccgccgcc gccgtaacca ccgcgat
//
LOCUS       DAOMXO010000652         1416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_54389_length_1416_cov_4.848641, whole genome shotgun sequence.
ACCESSION   DAOMXO010000652 DAOMXO010000000
VERSION     DAOMXO010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54389_length_1416_cov_4.848641"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PLH57_11260"
     CDS             <1..898
                     /locus_tag="PLH57_11260"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRK03233.1"
                     /translation="ALLVENEGSIWEYSYRGPGQEMRHFNFATPVVAIPTVAATSSET
                     DLYAVVTNADLNRKVAVFGESLIPKLAIIDPELTYTVGPEQTVDGAFDVINHVMESYL
                     STEKSAHFQDRLTEAVVETVVWALPRALEDPKNQEARSNLSWCAALALSGVFSGRDGG
                     WPIHALEHGLSAYTDVAHGRGLSCLLPRIMKFDAETIGPKIEHFNKKIFGTASLKDGL
                     ETYMKRVGAWVTLDEIAADHAFNGKKIAVPALIEKTIEHAFEVKPLLKRGEEPYLDNV
                     RKIYKQDARTILEWCRADSLDC"
     gene            complement(906..>1416)
                     /locus_tag="PLH57_11265"
     CDS             complement(906..>1416)
                     /locus_tag="PLH57_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03234.1"
                     /translation="TGMPFALFRLRERHFVVFRRGIYSFCTLFAMAIFARAAGDSGAH
                     VLRVTRPADAVRAETSWPIHCGAVAASVVDFLKDYELKFVGEEFLDVSGRMLVKRKRP
                     LLIESNPAERALAAEKFHAQREFRKNSDVVLHPEVCDELVDVEGVTDRRACEFTYYLK
                     ACGLLASLR"
BASE COUNT          379 a          389 c          334 g          314 t
ORIGIN      
        1 cgcccttctc gtcgaaaacg aaggctcgat ttgggaatat tcctaccgtg gccccggaca
       61 ggaaatgcgc cactttaatt ttgccactcc agtggtcgcc atccctacgg tggctgccac
      121 cagttcggaa actgacttat acgcggtggt cactaacgct gacctcaacc gcaaagtagc
      181 tgtattcgga gagtcgttga ttccaaagct cgcaattatc gaccccgaac ttacttacac
      241 agtcggcccc gaacaaactg tggacggggc atttgacgtc atcaatcacg tcatggaatc
      301 ttatctttcc accgaaaagt ccgcacactt tcaggaccgc cttacggaag cagttgttga
      361 aaccgtggtg tgggcgctcc cgcgagcact tgaagatcct aaaaaccaag aggcccgttc
      421 gaatttaagt tggtgtgccg cactcgctct ttcgggagtc ttcagcggta gagatggtgg
      481 gtggccaatt catgcgttag aacatggact gagcgcttac accgatgttg cgcacggacg
      541 tggactttcc tgcttattgc ccagaatcat gaagttcgac gccgaaacga ttggtccgaa
      601 gatcgagcac ttcaataaaa aaatatttgg cactgccagc ttgaaagacg gtctcgagac
      661 ctacatgaaa cgcgtcggcg cctgggtcac gctcgacgaa atcgccgccg atcacgcgtt
      721 caacggcaag aaaatcgcag tacccgcttt gattgaaaaa acaattgagc acgccttcga
      781 ggtaaaacct ttactcaagc gtggggaaga accttatctc gacaatgttc gaaaaattta
      841 caaacaagac gcacgaacga tcttggaatg gtgtcgcgcg gacagtttag actgctagtg
      901 gcgcactagc gaagactagc aagtagacca cacgctttga ggtaatacgt aaactcacag
      961 gcccggcggt cggtcacacc ttcaacgtcg accaattcat cgcagacttc cgggtgtagc
     1021 accacgtcag agttttttct aaattcgcgc tgagcgtgaa atttttcagc agctagtgcc
     1081 ctttcggcag gattggattc gataagcagg ggccgttttc gcttaaccaa catgcggcca
     1141 gaaacatcta aaaactcttc gccaacaaat tttaattcgt agtcttttaa aaaatcaacc
     1201 acactcgctg ccacagcccc gcagtggatt ggccaagaag tctccgccct gaccgcatct
     1261 gcgggccgtg taacacgcaa cacgtgcgct cccgaatcgc ccgccgcgcg tgcgaaaatc
     1321 gccatcgcaa acaacgtgca aaaactataa atgcctcttc gaaaaaccac aaaatgcctc
     1381 tctcttagcc taaataaagc aaacggcatg ccggtt
//
LOCUS       DAOMXO010000653         1413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_54595_length_1413_cov_3.226804, whole genome shotgun sequence.
ACCESSION   DAOMXO010000653 DAOMXO010000000
VERSION     DAOMXO010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54595_length_1413_cov_3.226804"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..>1413)
                     /gene="gpmI"
                     /locus_tag="PLH57_11270"
     CDS             complement(76..>1413)
                     /gene="gpmI"
                     /locus_tag="PLH57_11270"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRK03235.1"
                     /translation="GRIVYQELTRINRDIRNGAFRTNSVFKNALLQIQKSGGRLHLMG
                     LLSDAGVHSTWEHLKALVELASPLVPVTIHPFTDGRDTPPGSARGFLKDLELLVSAHP
                     GCNVGTIAGRYYAMDRDTRWDRTNLAFDALCGRSPVSPFKSDNGAAAAIQAFEDSYAK
                     GVTDEFLIPQRLQTKDRSSVGNNDGILFFNFRADRARQLTRAFTDSSFSEFDVSNRPK
                     LSTYVCMTEYHKDFGLPVAFPPQNLSRILPEIITENGLSQFRIAETEKYAHVTFFFNG
                     GKEKTYTRESRLLIPSPKDVPTYDLKPEMSLPEVSAKLTAELSRGETDFVLVNFANPD
                     MIGHTGSYEAALRALEAVDLALKSTITAALAQNYEVMITADHGNIEEMHDEHDQPHTQ
                     HTLNPVPFHLISNLRQVVLRDGILADLAPTILQLLGLPQPKEMTGKSLIKKII"
BASE COUNT          345 a          311 c          389 g          368 t
ORIGIN      
        1 gagcatcacc gccgctgcag aggaccgcaa tgtttttcat agagttgggt ttggtagatt
       61 tcatgaagtg tcctattaga ttattttttt tatcaaactc ttgccggtca tttcttttgg
      121 ttgaggtaag cctaaaagct ggagaatagt tggtgcaagg tcggccaata ttccgtctcg
      181 aagcactact tggcgtaggt tagagattaa gtgaaagggc acggggttga gcgtgtgttg
      241 ggtgtgcggt tgatcgtgtt catcgtgcat ctcttcgata ttgccgtgat cggcggtgat
      301 catgacctcg tagttctgtg cgagtgccgc cgttattgtg ctctttagtg caagatctac
      361 ggcctctagc gcgcggagag cggcctcata ggaccccgtg tggccgatca tgtcgggatt
      421 ggcaaaattt acgagaacga agtcggtttc gccgcgcgag agttcggcag tgagtttagc
      481 gctaacttcg ggaagactca tttccggttt taaatcgtaa gtaggcacgt ctttggggga
      541 cggaataagc aagcgagact cgcgagtgta ggttttttcc ttgccgccat tgaaaaaaaa
      601 ggtcacgtga gcatattttt cagtttcggc gatacggaac tgagacaacc cattttcagt
      661 gataatttct ggcaaaattc gggaaagatt ttgaggcgga aatgcgacag gcagtccaaa
      721 atctttatgg tattctgtca tacacacata ggttgagagc ttcgggcgat tagaaacatc
      781 gaattctgaa aatgaagaat cggtaaaagc tcgcgtaagt tggcgcgcac ggtccgctcg
      841 aaaattaaaa aacaatatgc cgtcgttatt gcctaccgat gaacgatctt tggtttgcaa
      901 gcgttggggt attaaaaatt cgtcagtgac gccctttgcg taggaatcct cgaaagcctg
      961 aatcgcggca gctgcgccgt tatcactttt aaagggacta accggtgacc tcccacaaag
     1021 agcatcgaag gccaaattgg ttcggtccca gcgggtgtcg cgatccatgg cgtagtatcg
     1081 cccagcgatc gtaccgacgt tgcaaccagg atgtgctgaa accagcagct ctagatcttt
     1141 taaaaaacca cgcgccgacc ccggtggggt atcacgcccg tcggtgaatg gatgaattgt
     1201 taccggaacc aacggggagg ccaattctac tagtgcctta agatgctccc aggtgctgtg
     1261 cactccagcg tccgacagca aacccatgag gtgcagtcga ccgcctgatt tttgaatctg
     1321 caggagggca tttttgaaaa cggagttcgt gcgaaacgct ccattgcgaa tgtcacgatt
     1381 gatacgtgtt agttcctgat aaacgatgcg gcc
//
LOCUS       DAOMXO010000654         1412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_54633_length_1412_cov_4.604274, whole genome shotgun sequence.
ACCESSION   DAOMXO010000654 DAOMXO010000000
VERSION     DAOMXO010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54633_length_1412_cov_4.604274"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="PLH57_11275"
     CDS             <1..1402
                     /locus_tag="PLH57_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRK03236.1"
                     /translation="GSYVYSYRDAEDAVLNSKEAFKLWRRTTLAERSELLRKYRDQLK
                     IKENLLAETIAREVGKPLWEAKQEVATMINKVDVTLAEGLKSVSDYEVPNILDGTLGA
                     CRYRPHGVFVVIGPFNFPGHLANGHIVPALATGNTVVFKPSEKSPGVGQIMAEAFNDA
                     GFPPGVFNMVQGEREVGRRLAVSEHTNGVLFTGSYEVGFKIKQDTLHQYWKLLALEMG
                     GKNSAIVCDDASIDWSIYEVLTGAFITAGQRCSATSRVFVHHTIFDAYVEKLHKRAKA
                     FAIGHPLDNPFMSALIDASSVEKYLKFVGIAAREGCDVLMRGKELEIDVPGNYVTPSI
                     CLVRDNSLQAVRKSVFQQTEIFGPMLAIYSFSEPEQAIELTNSVQYGLVASVFTSDKN
                     KYQKIAQELEVGLVNWNRSTVGASSRLPFGGVKKSGNHFPTAISAANYCVYPVASLEV
                     AEPKPLKATYTGLNWE"
BASE COUNT          379 a          308 c          366 g          359 t
ORIGIN      
        1 gggaagttac gtctattcgt atcgtgacgc cgaagacgct gttttgaatt ctaaggaagc
       61 tttcaaatta tggcgccgca cgacacttgc ggagcggtcg gaactgttac gtaagtatag
      121 agatcaatta aaaatcaaag aaaatctact cgcggaaacg atcgcacgcg aggtcggaaa
      181 gcccctgtgg gaagccaagc aagaagttgc gaccatgatt aataaagtcg acgttacatt
      241 ggctgagggt ctgaaaagcg tatcagatta cgaggtgccc aacatcctcg acggcacact
      301 tggggcctgt cgttatcggc cacatggggt ttttgtagta attgggccat ttaattttcc
      361 tgggcattta gccaatggcc acatagtgcc cgctctggcg acgggaaata ctgttgtctt
      421 caaaccttca gaaaaaagtc cgggcgttgg ccagataatg gcagaagctt tcaacgacgc
      481 tggatttccg ccaggtgttt tcaatatggt tcagggcgaa cgcgaagtag gcaggcgact
      541 cgcggtaagt gaacacacca acggagtttt gttcacgggg tcctacgaag tgggatttaa
      601 gattaaacaa gacactcttc atcagtactg gaaactgctt gcgttagaaa tgggcggaaa
      661 aaattctgcc attgtgtgcg atgacgcttc aatagactgg tcgatttacg aggtattgac
      721 gggggccttt attacggccg ggcaaagatg ttcggccacc agtcgggttt ttgtgcacca
      781 caccatcttt gacgcctatg ttgaaaaact tcataagcgc gccaaggcct ttgcaatcgg
      841 gcacccgctc gacaacccgt ttatgagcgc attaatcgac gctagttcag tggaaaaata
      901 tcttaaattt gtcggaatag ccgctcgtga aggttgcgat gtattaatgc gtggcaaaga
      961 gttagaaatt gacgttcccg gtaactacgt tactccgtcg atttgcttgg tgcgtgataa
     1021 ctctcttcag gccgttcgca agagtgtatt tcagcaaacc gaaatattcg gcccgatgct
     1081 agcgatttat tccttttcgg aaccagagca agcaatcgag ctgacaaata gtgtccaata
     1141 cgggttggtt gccagtgttt ttacgagtga caaaaacaaa taccaaaaaa tcgcacaaga
     1201 acttgaagtt ggtttagtga actggaaccg gtcgacggtc ggagcctctt ccagattgcc
     1261 ttttggcgga gtgaagaaaa gtggaaatca ttttccgaca gctatctcgg ctgcgaatta
     1321 ttgcgtgtac cccgttgctt cgctggaagt ggcggaaccg aaacctctta aagcgactta
     1381 taccggtttg aattgggaat aatccattca ca
//
LOCUS       DAOMXO010000655         1411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_54697_length_1411_cov_4.379794, whole genome shotgun sequence.
ACCESSION   DAOMXO010000655 DAOMXO010000000
VERSION     DAOMXO010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54697_length_1411_cov_4.379794"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..967)
                     /locus_tag="PLH57_11280"
     CDS             complement(65..967)
                     /locus_tag="PLH57_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HRK03237.1"
                     /translation="MKVTVLTRNRNLHSIRRLLKEGRALRAEVSIVDPLDCQLVVGSR
                     VNGLYVHDRPASRPDVVIPRIGTSITDYGLAVVTQFEILNVPVINSARAIAESRDKLR
                     CLQLLSAKNFGVPTTILSRSKRGTRLAFRQIGGVPAVLKLLQGTQGVGVMLAESMTSA
                     ESILDTMRGMEKDILLQQYIAESGGRDIRALVIGDKVVAAMRRQAKAGEFRSNIHRGG
                     EGSPIELSDSYKKLAVNSAKAVGLGIAGVDILESLTGPKVIELNSSPGFEGIEKTTGL
                     NIAKMILNYALQIARQKRRPARRR"
     gene            998..>1411
                     /locus_tag="PLH57_11285"
     CDS             998..>1411
                     /locus_tag="PLH57_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03238.1"
                     /translation="MTTKIIFLFLLFANFACSSIDANLPVDRSNPIRVDDLSFTPCAK
                     WNFSVPGQISNLSISESGKAILLSSSGEKNANGKIRLLDHMGKVLWSRTLKQPVKSQS
                     ISKDGRFIAISNYSDHLQFYTANGQLKWEANHFGKP"
BASE COUNT          358 a          356 c          307 g          390 t
ORIGIN      
        1 ttaacgtttc ggctttttgg gcgtactcga taaaaaaaga aatagtcgga attttttgcc
       61 gactctatcg tctgcgggcg ggtctgcgtt tttgccgagc gatctgtaac gcgtaattga
      121 ggatcatttt cgcaatattg agacccgttg ttttttcgat tccttcaaac ccgggtgatg
      181 aatttaattc gattactttg ggtcccgtaa gagattctaa aatgtcgacg ccagcgatcc
      241 cgagtcccac cgctttagcg gagtttaccg ctagtttttt gtaagaatct gacaactcta
      301 tcgggctacc ttcaccaccc cgatgaatgt tcgaacgaaa ttcgcccgcc ttcgcttgac
      361 gccgcatggc ggccacaact ttatcgccga taaccaaggc cctaatgtcg cgcccgccac
      421 tttcagcaat gtactgttgc aatagaatgt ctttttccat accgcgcatg gtgtctaata
      481 ttgattccgc cgatgtcata ctttcggcaa gcatcacacc aacgccctgc gttccctgta
      541 aaagttttaa aaccgccggt actcctccaa tttgacgaaa agccaagcga gtaccccgct
      601 ttgagcgact tagaatcgta gttggtactc caaagttctt ggcagatagc aactgcagac
      661 aacggagctt atcgcgcgat tccgctatcg ctcgtgctga gttgataacg ggcacgttta
      721 aaatctcgaa ctgggtgaca acggccaacc cgtagtcggt aatcgaagta ccaatacgag
      781 gtattaccac gtctggtctt gacgctggtc gatcgtgcac ataaagccca ttcacacgcg
      841 aaccgactac cagctgacag tcgagcggat cgacgatact tacttcagcc cgcagtgccc
      901 gcccttcttt taacagtcgt ctaatggaat gtagatttcg atttcttgtt agaacagtga
      961 ctttcatttt cagcgcctct caagcagaat gaacgcaatg accactaaaa tcatcttcct
     1021 ttttttgctg tttgcaaact tcgcgtgcag ttcaatcgac gccaatttgc cggttgatcg
     1081 ttcaaacccg atccgggtcg acgatcttag ctttacgcca tgcgcaaaat ggaacttctc
     1141 tgtgcccggt caaatatcta atttatctat ttcagagagt ggcaaggcaa ttcttctaag
     1201 ttctagtggc gaaaaaaatg cgaatggcaa aattcgactt ttggaccata tgggcaaggt
     1261 gctttggagt cgcactctca aacaacctgt aaaaagtcag agcatttcca aagacggccg
     1321 ttttatcgca atctcaaact acagcgatca tttgcagttt tatacggcaa atggccaatt
     1381 aaagtgggag gctaatcatt ttggtaagcc c
//
LOCUS       DAOMXO010000656         1410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_54786_length_1410_cov_2.848708, whole genome shotgun sequence.
ACCESSION   DAOMXO010000656 DAOMXO010000000
VERSION     DAOMXO010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54786_length_1410_cov_2.848708"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1410)
                     /locus_tag="PLH57_11290"
     CDS             complement(<1..>1410)
                     /locus_tag="PLH57_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03239.1"
                     /translation="KDAHVVKVFNRTRNLERAIAIRRYAMADGFVTAGLAYWGTEFLL
                     GELAKMDSSTEAGLQTPVNLLPENPDIRRMLSLTMPDAPKWIQGLVQWALSDIDQTKK
                     QSQRNQLKARVWAMLDYVDGLYDVVQEGQKDREVQKVFREIDGRLKNRKPYAWHSTRL
                     RMAEFYRAIQTTQNPDSRAFKTINAVVRLQNVLQIIFSQTAIAVTEEHIAPILASLLG
                     GSRTSLDKSRKIFSEGREYLKMRPNLLITLFGPPSMRKEEFRTLLKNPTNYKEYLEGI
                     KSDVVNDYPGLFLALPYPTRIGGVELWQGEKSRPKAPITLAEIIRVLAPKFCSEHEKA
                     SCDSGMFAQLSGGGTSWIKSLAKRAKKKSFDSEFDTPIEVPQLIEAVAGEYSTTPTGR
                     GLVLWLLDKGTGDDRQTNGYFSSKFLARDFYGAAFHKNSDGGSAHSLSTGLRVVVSQL
                     MGAFLKAAEEANYRNLTRL"
BASE COUNT          347 a          364 c          330 g          369 t
ORIGIN      
        1 atgagtcggg ttaagtttcg gtagttcgct tcttccgccg cctttaggaa ggctcccatc
       61 agctgagaga caacaactcg aagtccggta gaaagggagt gtgccgatcc tccgtcggag
      121 tttttatgaa aagctgcgcc atagaaatcc cgcgcaagaa atttcgaact aaagtaacca
      181 ttggtctgac gatcatcccc agtgccttta tcgagcagcc aaagcaccag cccacgccca
      241 gtcggtgtag tggaatactc ccccgcaact gcttcgatga gttggggcac ttcgatagga
      301 gtgtcgaact cggaatcaaa agattttttc ttagcgcgtt ttgcaaggga tttaatccaa
      361 ctcgttcctc cgccactgag ttgtgcgaac atcccggaat cgcaactcgc tttttcatgt
      421 tccgaacaaa acttgggagc taacacgcgg atgatttccg cgagcgtgat gggcgccttg
      481 ggtcgacttt tttcaccctg ccacaactca acaccaccaa ttcgagttgg atacggcagt
      541 gccaagaaaa gacccggata atcatttaca acgtcacttt taataccctc gagatactct
      601 ttgtagttcg tcgggttctt aagcaatgtg cgaaattctt ctttgcgcat gctcggtggt
      661 ccaaaaagtg tgatcagtaa atttggacgc atttttaagt actcgcgccc ttcgctaaat
      721 atctttcgag acttgtcgag cgacgtgcgt gaaccaccca aaagagaagc caatattgga
      781 gcgatgtgtt cttcggttac cgcgattgcg gtttgactga aaataatctg taacacattc
      841 tgcaaacgaa ccacggcatt aatggtttta aaggcgcggc tatcggggtt ttgagtggtc
      901 tgaatcgcac gataaaactc tgccattcgc aaccgtgtcg aatgccatgc atatggtttg
      961 cggttcttta agcgcccatc gatttctcta aaaactttct gaacctcgcg gtctttctgc
     1021 ccctcttgaa cgacgtcgta caaaccgtcg acgtagtcca acatggccca cactcgcgcc
     1081 ttcaattgat tgcgctggga ttgctttttg gtttggtcga tatcgctcag cgcccactgt
     1141 acaaggcctt gaatccattt tggggcgtcg ggcatagtta aactgagcat gcggcgaata
     1201 tcgggatttt ccggcaataa atttactgga gtctgcaagc cggcctccgt cgacgaatcc
     1261 attttcgcca actcacccaa caaaaattcg gtaccccaat aagcaaggcc cgcggtcaca
     1321 aaaccatccg ccattgcata gcggcgaatc gcaatcgcac gctcgagatt acgggtgcga
     1381 ttgaatactt ttacaacgtg agcgtctttt
//
LOCUS       DAOMXO010000657         1409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_54830_length_1409_cov_4.014032, whole genome shotgun sequence.
ACCESSION   DAOMXO010000657 DAOMXO010000000
VERSION     DAOMXO010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54830_length_1409_cov_4.014032"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /gene="htpX"
                     /locus_tag="PLH57_11295"
     CDS             complement(<1..602)
                     /gene="htpX"
                     /locus_tag="PLH57_11295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRK03240.1"
                     /translation="MLTHMRRYFLFFVVNIGIVLTISLVLNLLGVSPYIEARGLNMES
                     LAIYCLIWGMAGAFISLAISRIMAKMMMGVKVIDPMTRDSELQWLVSTTHDFAKAAGL
                     RKMPEVGIYESPELNAFATGPTKNRALVAVSTGLLRRMNRSEIEGVLAHEVSHISNGD
                     MVTMTLIQGIMNAFVMFLARLIAFALTQNMKDESRPMARM"
     gene            complement(689..1207)
                     /locus_tag="PLH57_11300"
     CDS             complement(689..1207)
                     /locus_tag="PLH57_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRK03241.1"
                     /translation="MSEKIHETHLAQWIRLAEPVLSGALDAVAVVDLRNQLAYANPSM
                     KTLLKLRPSHLAGKKKFCDCITLSICAKKCQIEELIQTATAFRFDESPAVISSKADST
                     KLRVNIRGVAVPNPDTKSSTKTIGAVITLRDTSADVLVQAKYHKLAELLKERDETIAE
                     LRRLNAVLRRKP"
     gene            complement(1207..>1409)
                     /locus_tag="PLH57_11305"
     CDS             complement(1207..>1409)
                     /locus_tag="PLH57_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03242.1"
                     /translation="YSIELPYYCTKCDAASNIFLETSKIPLVNGQPKAPQKKCHRCGE
                     ALEIDAFEEEYLSFLKYMKAKK"
BASE COUNT          364 a          306 c          358 g          381 t
ORIGIN      
        1 aacattctag ccattgggcg actttcgtct ttcatgttct gagtcagcgc aaatgcaatg
       61 agacgagcaa gaaacatgac gaacgcgttc atgattcctt gaatgagcgt catcgtcacc
      121 atgtcgccat tggaaatatg agaaacctca tgggctaaaa ctccctcgat ttccgaccga
      181 ttcatgcggc gtaaaagccc agttgatacc gcaacgagcg cgcgattttt agtcgggccg
      241 gtagcaaagg cattgagctc ggggctttcg taaataccaa cttcgggcat ttttcgcaga
      301 ccagcggctt ttgcaaaatc atgggtggtg gatacgagcc attgaagctc actatcgcgc
      361 gtcattgggt caattacttt tacacccatc atcattttgg ccatgatgcg agagatggcg
      421 agtgaaataa acgccccggc cataccccag attaagcagt agatcgccag agattccatg
      481 ttgaggccac gcgcctcaat atagggtgat actcctagga gatttaggac caacgaaatc
      541 gttaatacga ttccaatgtt caccacgaaa aacaaaaagt agcggcgcat gtgtgtcagc
      601 ataattggcg atctcctcta aatctgaata gctctataaa tgtggttatt ctacgggggt
      661 tgtcaaatcg aagtatttct gtcggaaact agggttttcg gcgcaaaaca gcgttgagac
      721 gccggagttc ggcaatggtt tcgtcgcgtt ccttcaaaag ttcggcgagc ttgtggtact
      781 tggcctgaac gagtacgtca gccgacgtgt cacgtaaagt gatgacagca ccaattgtct
      841 tggtgctcga tttggtgtcg ggattcggaa cggcgacgcc acggatattg actcggagct
      901 tggtggagtc cgctttgctc gaaataaccg cagggctttc gtcgaagcga aacgcagtag
      961 cggtttgaat taactcttcg atttgacatt ttttggcaca gatcgagagg gtgatgcagt
     1021 cgcagaactt ttttttgcct gcaaggtgcg atgggcgcaa tttaagtaga gttttcatcg
     1081 acggattggc gtaagcgagt tggtttcgta aatccacaac agccaccgcg tctagagcac
     1141 ccgagagtac gggttctgcg agccgaatcc attgagcgag atgtgtttcg tgaatttttt
     1201 cagacattat ttttttgctt tcatgtattt aagaaagctt aaatattctt cttcaaatgc
     1261 gtcgatctca agtgcttcgc cacaacgatg acattttttt tgaggggcct taggctggcc
     1321 atttactaaa ggaatttttg aggtctcaag aaaaatattc gaagcagcgt cgcacttggt
     1381 gcaatagtag ggaagttcaa tgctataaa
//
LOCUS       DAOMXO010000658         1408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_54894_length_1408_cov_3.991870, whole genome shotgun sequence.
ACCESSION   DAOMXO010000658 DAOMXO010000000
VERSION     DAOMXO010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54894_length_1408_cov_3.991870"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..926)
                     /locus_tag="PLH57_11310"
     CDS             complement(153..926)
                     /locus_tag="PLH57_11310"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HRK03243.1"
                     /translation="MSKTIVRAYGSVSDPGPSRDLNEDAVIAVPAHKLFGVADGFGGT
                     GIGDVAATKTLEDVKYFVENGLGDSEVTLPFIYRTYLSASANLIFNALLYANERLCAE
                     NKSKPLNLRAGASLLFAFFSGDHMTLAHAGSCAAYLVRRGRAMQIAKPRSFNAVKGVF
                     PGSWNGKWAFPLMALGIAKDIEPEIIELKIQRGDLLMLATDGVHGRLTDEDFSQSYSM
                     LSEKTPLDILIHKENQRLITTANQKGNHDNQAVITMVCA"
     gene            complement(919..>1408)
                     /locus_tag="PLH57_11315"
     CDS             complement(919..>1408)
                     /locus_tag="PLH57_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03244.1"
                     /translation="EAIDKMNEAPLMGYQDEGMLNEAKQIASEARRRLREDVKPFLED
                     AVRAMASGDMAKARDGYMAALRIDARNKTARAALDGIKKMLFERARRIYTEGLIAEGV
                     SSYREARSKFKECLDIAMPDSVYHGRCSRKFKRFELLDRHVAGGSDTSNVEIEGDGGS
                     DE"
BASE COUNT          337 a          372 c          306 g          393 t
ORIGIN      
        1 gattcatccc gaattagggt tcgaagctcg cgtcgaacgt agtctttgct ttttttgaga
       61 aacaccaatg ctctcatatt tagatagccc tcagagccct tatcggactc tccctataaa
      121 aattataggg tttattgtct attcctgtca atttatgcgc aaaccattgt aatgactgcc
      181 tgattatcat ggttaccctt ttgattggcg gtcgttataa gcctttgatt ttctttgtga
      241 attagtatgt cgagcggggt tttctcgcta agcattgaat aagactggga aaaatcttcg
      301 tcagtcaaac gaccatgtac cccgtcggtg gcgagcatta aaaggtcgcc tcgttggatc
      361 tttaactcaa taatttccgg ctcaatgtcc ttagcaatgc ccaatgccat gagcggaaac
      421 gcccatttac cgttccaaga ccccggaaac acccctttga ctgcgttaaa cgaacggggc
      481 ttggcaattt gcatggccct accgcggcgc actaaataag ctgcacagga accagcgtgg
      541 gccaacgtca tatgatcgcc ggaaaaaaag gcaaacaaca gactggcgcc agctcgcaag
      601 ttaagtggct tcgatttatt ttcggcacag agacgttcat tggcataaag aagcgcattg
      661 aaaataagat tggcgcttgc actcaaatag gttcgataaa taaaaggtag cgtaacttct
      721 gagtcgccta ggccgttttc tacgaaatac tttacgtcct cgagcgtttt agtagcggcc
      781 acatctccaa tgccagtacc gccaaatccg tcagccacgc cgaagagttt gtgtgcgggt
      841 actgcgatca ccgcgtcttc atttaaatct ctactcggtc ctgggtccga cacgctccca
      901 taggcccgaa cgatcgtctt actcatcgct gcctccatcg ccttcaattt ctacgtttga
      961 agtgtcggag ccaccggcca catggcgatc caaaagttca aatcgtttaa attttcggga
     1021 gcatcgccca tgataaacgc tatctggcat tgcgatatcc agacattctt taaatttcga
     1081 acgagcttcg cgatagctgc tcacaccttc ggcaattagc ccctcagtgt aaattcgtcg
     1141 ggcgcgttca aaaagcattt tcttgattcc gtctaacgcc gctcgggccg ttttatttcg
     1201 cgcgtcgatc cgaagtgctg ccatataacc gtctcgggct tttgccatgt caccactagc
     1261 catagcccga actgcgtctt cgagaaatgg tttaacatct tcccgaagcc ttctgcgcgc
     1321 ttcgctcgct atttgtttgg cttcattcaa catgccctcg tcctgataac ccatgagcgg
     1381 cgcttcgttc attttgtcga ttgcttca
//
LOCUS       DAOMXO010000659         1405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_55047_length_1405_cov_5.219259, whole genome shotgun sequence.
ACCESSION   DAOMXO010000659 DAOMXO010000000
VERSION     DAOMXO010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55047_length_1405_cov_5.219259"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1405)
                     /locus_tag="PLH57_11320"
     CDS             complement(<1..>1405)
                     /locus_tag="PLH57_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03245.1"
                     /translation="SVFCSGEGLGKLKNGRLFWISYLVLVSFTFETVLWTAGDWFPKA
                     VSGLFSLTAAASEVPPSKPAPRGPVDELEQAAKIAFGDSLWEPGELEKLAQPIRISHD
                     DPILDQDPFYLRSQRWVPSTSGALTGAPSAATDVYFQTFENGVEVSIPGLAKSWSLDV
                     PLQPLLATEDYLFFSARDPSLFEAKTIGVDAETELGGGVFIVVMNDLLQSLPDATPVP
                     VFFFPLYGDAWDVDVRAHEEPALGAVHFVDAGDEVVTVRNDHIAQVIQVERGNLLIAR
                     LDATKARLTQLFRDPDFLEKFKNFSAKSSAFLSDGQAIHLDQAAPFLADLEKLVGDVE
                     LFPGRGATAGFGVLYVGYDLDHSTSSLLTAQAAGHRDVLQRVLDFASPRSAFAGGDSE
                     DPAQLMRENYAIWRLVFAILGMLLVFSFTTRYAVKSIRQRLREVNDLQDRREVEAAQA
                     AARKRAEKIEAKRKKSGR"
BASE COUNT          376 a          387 c          336 g          306 t
ORIGIN      
        1 cggccagact tttttcgctt ggcttctatt ttttcggcgc gttttcgggc agctgcctgc
       61 gccgcttcaa cttcgcgtcg gtcctgcaaa tcgttcacct cgcgaagtcg ctgacgaatg
      121 gattttaccg cgtaacgagt agtaaaagaa aaaaccaaaa gcattcccaa aatggcgaaa
      181 accaggcgcc aaatggcata attttctcgc attagttgcg cagggtcttc tgaatcgcca
      241 cctgcgaaag cggaccttgg gctagcaaaa tctaatacgc gttgcaaaac gtcacggtgg
      301 ccagcggcct gagctgttag taggctcgaa gtgctgtggt ctaaatcata acctacgtag
      361 agaaccccaa atcccgccgt ggctccacgc ccaggaaaca attcgacgtc tcccacaagt
      421 ttttcaaggt ccgccaggaa cggtgcggcc tggtctaaat gaattgcttg cccgtcactt
      481 aaaaaagcac tcgatttagc cgaaaaattc ttaaatttct ccaaaaagtc tggatcgcgg
      541 aaaagctgag tcagccgagc tttcgtggca tcaagccgcg cgatcagtaa attaccgcgt
      601 tcaacttgaa tgacttgcgc gatgtgatca ttgcgaacgg taacaacttc gtctccggca
      661 tcaacaaagt gcacggcacc caatgctggt tcttcatggg cacgcacatc aacgtcccac
      721 gcgtcaccat aaagtggaaa gaaaaacacc ggcaccggag ttgcgtctgg aaggctctgt
      781 aaaaggtcat tcatcactac aatgaaaacc ccgcccccca actccgtttc cgcatccact
      841 ccgattgttt tcgcctcaaa aaggcttgga tcacgtgcgg aaaaaaacaa atagtcctcg
      901 gttgcgagca atggctgcaa aggaacatcg agcgaccaac ttttggctag ccctgggatc
      961 gacacttcca cgccattttc aaaggtctga aagtatacgt cagtcgcagc cgaaggggca
     1021 ccagtcaaag cacccgaggt cgacggcacc caacgctggg accgcaaata gaaagggtct
     1081 tggtctaaaa ttggatcgtc gtgagaaatg cgaatgggct gtgctagttt ttcaagctca
     1141 ccgggttccc acagtgagtc gccgaaagcg attttggcag cctgctctaa ctcgtcaact
     1201 gggcctctag gcgcgggctt cgagggtgga acttcagaag ccgcggccgt taggctaaac
     1261 aggcccgata cagctttagg aaaccaatct cccgccgtcc atagtaccgt ttcaaaagta
     1321 aaagagacca gcacgaggta gctgatccaa aaaagacgcc cgtttttcaa ctttccgagt
     1381 ccctctccac tacaaaaaac actcg
//
LOCUS       DAOMXO010000660         1404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_55109_length_1404_cov_4.893996, whole genome shotgun sequence.
ACCESSION   DAOMXO010000660 DAOMXO010000000
VERSION     DAOMXO010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55109_length_1404_cov_4.893996"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLH57_11325"
     CDS             complement(<1..538)
                     /locus_tag="PLH57_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK03246.1"
                     /translation="MRLRQRNASLAFIFITIAIDAIGLGIVIPVMPDVIRRFIFDEAL
                     VSRTYGYFIAIYALLQFTSSPVLGGLSDRFGRRPILLIALLGAAIDYAFMAYAPTLFL
                     LFVGRVISGISGASFTVASAYIADISNDQNRSKNFGVIGAGFGLGFVLGPALGGWLAQ
                     FGSQTPFLAAAGFNFLNFL"
     gene            complement(560..>1404)
                     /locus_tag="PLH57_11330"
     CDS             complement(560..>1404)
                     /locus_tag="PLH57_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRK03247.1"
                     /translation="FRAIATEGGIARAAEKLRLGQPTLSAQLKHLEDMVGRPLFERRN
                     RKLILTESGKAALDYANEIFRLGDEMLEVLKDRNTENQHHLQIGALDSVPKNVILAMV
                     MEAHKIGPCTVSILEGKGDELIRELRAHKIDLILSNYPAQALEEAQVFSKSVAKLPVS
                     VYGSKKFANLKNKFPESLTGQPFVVPTLHSKLRHDLNHYFKLHGIRPQMVAETQDTSL
                     QKLLAEHGVGIAPFSEATGVSERNLLRLGRLDSVYEEIWLISAQRKLENPIAAKLLNS
                     FSLH"
BASE COUNT          354 a          329 c          386 g          335 t
ORIGIN      
        1 aaagaaaatt caaaaaatta aatccggccg ctgccaagaa cggagtttgc gatccgaatt
       61 gcgccagcca cccgccgagt gctggcccca atacgaaacc caagccaaag cccgctccga
      121 tcacaccaaa attcttagac cgattttggt cgtttgaaat atcagcaatg taggcgctcg
      181 ccaccgtgaa actcgcgccg gaaatgccag agataacgcg tcccacaaat agaagaaaaa
      241 gcgtgggagc ataagccatg aacgcataat ctatggcggc gccgagtaag gcgattagca
      301 aaatcgggcg tcggccaaaa cggtccgata ggccaccgag gaccggtgaa ctggtaaatt
      361 gtaggagcgc gtagatggcg atgaaatagc cgtaggttcg cgatactaga gcttcgtcga
      421 aaataaatct tcgaatcaca tcgggcatta ccggaatgac gattccgagc ccgatcgcgt
      481 ctatagcgat agtaatgaaa ataaaggcga gactcgcgtt tcgctggcgc aggcgcatgg
      541 gaagaaccta ctcctaaatt cagtgaagcg aaaacgaatt taggagtttg gcggctatcg
      601 gattttcgag tttccgttgg gcggaaatca accagatttc ttcgtaaaca ctatcgagtc
      661 ggcctagccg tagaaggttg cgttcggata ctcctgtcgc ttccgaaaat ggggctatac
      721 ccacgccgtg ttcggctagc agtttttgga ggctggtgtc ctgggtttcg gccaccattt
      781 gcgggcgaat gccgtggagt ttgaaatagt ggttcaggtc gtgacgtagt ttactgtgca
      841 gtgtgggtac cacaaaaggc tgtccggtta gtgactcggg aaacttgttc ttgagattgg
      901 caaatttctt agagccgtag accgatactg gaagttttgc gaccgacttc gaaaaaactt
      961 gggcttcttc gagtgcttga gctggataat ttgaaagtat gaggtcaatt ttatgggcgc
     1021 gtagttcacg aataagttcg tcgcccttgc cttcaagaat tgaaaccgta catggtccaa
     1081 ttttgtgagc ctccatgacc atcgcaagaa tcacgttttt gggtacgcta tcgagggcac
     1141 caatctgtag gtgatgttgg ttttcggtgt ttcgatcttt taacacctct agcatttcgt
     1201 cgcccaagcg aaatatttcg ttggcatagt cgagcgcggc cttaccgctt tcggtaagga
     1261 ttaacttgcg attgcggcgt tcgaaaaggg ggcgcccgac catgtcttcg aggtgcttga
     1321 gctgggcgct aagggtaggc tgaccaagcc ggagcttttc cgcggcgcga gcaatgcccc
     1381 cctccgtggc aatagcccga aagt
//
LOCUS       DAOMXO010000661         1403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_55159_length_1403_cov_4.986647, whole genome shotgun sequence.
ACCESSION   DAOMXO010000661 DAOMXO010000000
VERSION     DAOMXO010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55159_length_1403_cov_4.986647"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PLH57_11335"
     CDS             <1..1256
                     /locus_tag="PLH57_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK03248.1"
                     /translation="MEFVLPIYKKGEAPVGARSSALLGLGLWVYDALSLFRTPFFHGR
                     HSLDTLKGLFPGVRAQGLRGGYYYADAMMLDDELVLEVLLDSVRRGACALNYCAATRV
                     DPRDRDGLYRIHLETCDPAVSPPPNEQSASESLQVRAREVIMCVGPWTEEVSGIVAGG
                     AQKKLRPSRGVHLVFDWARLPVERCLVMYAPDGRIIFAIPRKDLGDEAAVTIVGTTDA
                     PEHGAIDQVEATSDDVEYLFGVLKEYFPNSKLAKSDVLMTYAGVRPLIDDGAENEAKT
                     SREHEIWKNEQGVVFMAGGKYTTFRKISEEIADFAFPRSKSDAKESRQPLSTPDDYAK
                     RFSGAPLWGRFTEGWVEWKLKHHAPLCLDDVVFRRLPLWMHGQKIPKDVIERIQSISA
                     KTWNQTSMSGQANQTREHIARAFQW"
BASE COUNT          350 a          333 c          397 g          323 t
ORIGIN      
        1 cgatggagtt cgtcttgcca atttataaaa aaggcgaagc gcccgtcggt gcgcggagtt
       61 cggcgttact cgggctaggg ctttgggttt acgatgcgtt gagcctgttt cgaacgccgt
      121 tttttcacgg gcgccattcg ctcgacactc tgaagggatt attccctggc gtgcgcgcac
      181 aggggctgcg agggggatat tattacgccg acgccatgat gctcgacgac gagttggtac
      241 tagaagtgtt gctcgatagt gtgcgacgcg gcgcgtgtgc gttgaattac tgcgcggcca
      301 ccagagtgga tccgcgggat cgcgacggtc tgtatcgaat tcacttggaa acctgcgacc
      361 cggcagtttc tccgccccca aacgagcaga gcgcgagtga gtcgctgcag gtgcgagcgc
      421 gagaagtgat catgtgtgtg gggccttgga ccgaagaagt cagcggaata gttgcagggg
      481 gagctcaaaa gaaattgcgc cctagtcgcg gagttcattt agttttcgat tgggcgcgac
      541 ttccggtcga gcgctgtctg gtcatgtacg cgcccgacgg gcgaataatt tttgcaattc
      601 cacgtaaaga cctgggcgac gaagctgccg tgacgattgt gggtaccacc gatgctccag
      661 aacatggagc catcgatcaa gtagaagcta cgagcgacga tgtggaatat ttatttggcg
      721 tgttaaaaga atattttcca aatagtaaac tcgcgaaaag cgacgtctta atgacctacg
      781 ctggagttcg accgctgatc gacgatggtg ccgaaaatga agctaaaact tcgcgtgaac
      841 acgagatttg gaaaaatgaa cagggcgtag tgttcatggc cggcggaaaa tacacgacgt
      901 ttagaaagat atctgaagaa atcgccgatt ttgcgtttcc ccggtctaaa agcgatgcca
      961 aagaatcgcg gcagccgctt tccactccgg acgactatgc taaacgtttt tcgggcgctc
     1021 ccttgtgggg ccgttttacc gaaggctggg tagagtggaa gctcaaacat catgctccct
     1081 tgtgtttaga cgatgtggtg tttcggcgcc taccattgtg gatgcatggg cagaaaattc
     1141 ccaaagacgt gatcgagcgc attcaatcca ttagtgcaaa aacctggaat cagacctcaa
     1201 tgtcgggtca agccaatcaa actcgcgaac atattgcgcg agcttttcag tggtaaaact
     1261 actcacctag aagttttctt cctactaagt acaaaacgaa gtgcggccga tatagaagta
     1321 tatgtttagc caagacgcac ttcgaaagat gctcaaacgc acgccctatg tcgccaaaaa
     1381 gaaacccgag atcgtgtaca ccc
//
LOCUS       DAOMXO010000662         1402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_55228_length_1402_cov_4.544915, whole genome shotgun sequence.
ACCESSION   DAOMXO010000662 DAOMXO010000000
VERSION     DAOMXO010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55228_length_1402_cov_4.544915"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1402
                     /locus_tag="PLH57_11340"
     CDS             <1..>1402
                     /locus_tag="PLH57_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03249.1"
                     /translation="NKPWSFAYSLRGIYRGLEELAGLFVGLPTRAPLYGDLEVRKLIQ
                     THFTDALKKAPADLLEHRFLNANLMGKAGPIVLAIESRLDHNATNAEFQDALKDSPEL
                     EALSPRLGEALANRVRTIELLEQAQRNYESRLESWVLEQYEQLKIKAPIRARDTEWAA
                     SKIQNFAFSSGSRMPSVYTAYLGVSDQLEEEGLNKEARFVSLSTALMAHAQALLHQWN
                     REQQPARKFEFKIPTLLVRNWDVEKVGDRFYIRDFSTATVNTGGFMWRAKNLAFRSIA
                     RFKGALQFLIFRNVINGVFGLKSLFKKKPFAPHWEANQTTGEIEPSGERGTLRSRVAA
                     SREKRRELLKDHRNSQETGFMGKNFERVTLFFKADLGYGLAAPLMIWTLQPAATAANL
                     VASSALSVAAIPGVALVGLAEHARNILISDHDVARERETRKSRFFPGPKAALATAGHA
                     TIGLGGATVGLGKNLAV"
BASE COUNT          351 a          382 c          354 g          315 t
ORIGIN      
        1 tcaacaaacc ctggagcttc gcctattcgt tacgcggaat ttatcgcgga ctcgaagaac
       61 tcgcgggctt atttgttggt ctgccaactc gcgcgccact ttacggcgac ttggaagttc
      121 gaaaactaat acaaactcat ttcacggatg cgctaaaaaa ggctcccgcc gatctgctag
      181 agcaccgttt cttaaatgcc aatttgatgg gaaaagctgg tcctatcgtt ttggccattg
      241 aatcgcgcct cgatcacaac gccactaatg ctgagtttca ggacgctttg aaagacagtc
      301 cggagcttga agcgcttagt ccgcgcctcg gcgaagctct tgccaatcga gtgcgcacta
      361 tcgaactgct tgaacaggct caacgcaact atgaatctcg acttgaatcc tgggtgctcg
      421 agcaatacga gcaactgaaa ataaaggctc caatacgtgc cagagatacg gagtgggcag
      481 ccagtaagat tcagaacttc gccttttcct ccggctcacg aatgccaagc gtttacaccg
      541 cttacttagg cgtttcggat caactcgagg aagagggcct aaacaaagaa gccagattcg
      601 taagtctctc caccgcactc atggcccacg cacaggctct actgcaccaa tggaatcggg
      661 aacaacagcc cgctcgcaag ttcgaattta aaattcctac gctcttggtg cgaaattggg
      721 atgtggaaaa agtcggagac cgcttttata ttcgtgattt ttcgaccgct actgtaaaca
      781 ccggtggctt catgtggcga gccaaaaatc tcgcttttcg atcgatcgcg agatttaaag
      841 gagccctgca gtttttaatc ttccgaaacg taatcaacgg ggtttttgga ctaaaatcgc
      901 tatttaaaaa gaaaccgttc gcaccgcact gggaagcgaa tcaaaccact ggggagatcg
      961 aaccctcggg cgaacgcggt actttgcgtt cgcgagtggc ggcctcgcgc gaaaagcgac
     1021 gtgaactatt aaaagatcac cgtaactcgc aggaaactgg cttcatgggt aagaacttcg
     1081 aacgagtgac gctgtttttt aaagccgatc tcgggtacgg gttggccgca ccgctcatga
     1141 tctggacgct gcaaccagcc gctacggctg ccaacctcgt cgctagctcc gcccttagtg
     1201 ttgctgccat tccgggcgtc gcgctggttg gccttgcgga gcacgcgcgc aatatactta
     1261 tttccgacca cgacgtggca agagaaaggg aaactcgcaa gtcgcgtttt tttccaggac
     1321 caaaagcagc gcttgcaacc gccggccacg ccacaatcgg actcggcggc gccaccgtcg
     1381 ggctaggtaa gaacctcgcc gt
//
LOCUS       DAOMXO010000663         1401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_55278_length_1401_cov_4.060921, whole genome shotgun sequence.
ACCESSION   DAOMXO010000663 DAOMXO010000000
VERSION     DAOMXO010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55278_length_1401_cov_4.060921"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLH57_11345"
     CDS             <1..595
                     /locus_tag="PLH57_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03250.1"
                     /translation="REPPAAEALDLPIDQIESHLESARLVHSGGRNWLVSPALNEVDG
                     FATCPQWERVYQIPQHYVWKAKQKIDGQKVQEKNADAVVRAEFLQEIFDRYVGNDLDD
                     KTTKALHGHLSDCTTCRKGFVHFRKTYNDWVTTIPTVEADAALQKHLDGVSKQATRQL
                     AGNPTAWLAFKQSLRNPQLRAILVLLAAGLIYVYFKN"
     gene            complement(592..>1401)
                     /locus_tag="PLH57_11350"
     CDS             complement(592..>1401)
                     /locus_tag="PLH57_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRK03251.1"
                     /translation="VQISPDDLARLKKTILANAERPALFQQELREELKSKEIPKDARF
                     IAPHNQIVPEETVAGAQPDAPKAGRVGRSDVGAEPRVKNTPLKLSQLGLGSKPLPKPI
                     MNAEDISGHRGPQQPRGEDGRPVGREDARLKRGNENLLNAIESEYYSFFARFEEPIVR
                     NWYFLLRTHEKRIRAEIAQISKSGEGEFPVTIEFVIDRQGNFNSISVLESSRVPTLDS
                     ATMQAVRKLGSLPNPPPGLFKDGLNFSYRLQFLVRVSGDPMGDSSPDLRWY"
BASE COUNT          334 a          336 c          366 g          365 t
ORIGIN      
        1 gagagagcct cccgccgccg aagcgctaga tctgccgata gatcagatcg aaagccacct
       61 cgaatcggcg cgtctggtgc attctggtgg gcgcaactgg ttagtgtcgc ccgccctcaa
      121 cgaagtcgat ggttttgcta catgtcccca atgggaacgc gtgtatcaaa ttccgcaaca
      181 ttacgtatgg aaagcaaaac aaaaaattga tggccaaaag gttcaagaaa aaaatgcgga
      241 cgctgttgtg cgagcggagt ttttacagga aatttttgat cgctatgtgg gcaacgactt
      301 agacgacaaa accaccaagg ctcttcatgg ccatttgagc gattgcacca cttgtcgcaa
      361 gggcttcgta cattttcgga aaacttacaa cgattgggta actacaattc ctacggtcga
      421 agccgatgca gcattgcaaa agcatctaga tggcgtaagc aaacaagcga ctcgccagct
      481 tgcgggcaat ccgactgcgt ggcttgcgtt caaacaatcg ctgcggaatc cccagttgcg
      541 cgccattttg gttctactag ctgcgggact aatctacgta tactttaaga actagtacca
      601 gcgcagatca gggctggaat cgcccattgg gtcacccgaa acgcggacta aaaattgcag
      661 tcgataggaa aaatttaaac catctttaaa tagtccaggt ggtgggttcg gaagcgaccc
      721 tagctttcgt actgcctgca tggtggccga atctagtgtt ggcacacgag aagattcaag
      781 caccgagatg ctgttaaagt tgccctggcg gtcaattaca aactcaatcg tgacaggaaa
      841 ttcgccttcg cccgatttac ttatttgggc gatttccgct cgaatgcgct tttcgtgagt
      901 gcgcaaaaga aagtaccaat tgcgcacgat cggttcttca aaccgcgcga agaacgaata
      961 atattcgctt tcgatcgcgt ttaggaggtt ttcattaccg cgttttaaac gggcgtcttc
     1021 tcggcccact ggtcgtccgt cttcgccacg aggctgttgc ggtccccggt ggccgctaat
     1081 gtcttcagca ttcatgattg gcttgggtag cggtttggac cccagtccta actgcgaaag
     1141 ttttagaggt gtatttttga cccgtggttc cgctccaaca tcgcttcgac caacgcgtcc
     1201 agctttgggg gcgtcgggtt gggctcccgc cactgtttct tcgggtacga tttgattatg
     1261 gggggcaatg aaacgtgcgt ctttaggaat ttctttggac tttagttctt ctcgcaactc
     1321 ttgttggaat aaggcggggc gttcggcatt tgcgagaata gtttttttta gtcgtgcaag
     1381 atcgtcaggt gatatttgta c
//
LOCUS       DAOMXO010000664         1392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_55777_length_1392_cov_3.452506, whole genome shotgun sequence.
ACCESSION   DAOMXO010000664 DAOMXO010000000
VERSION     DAOMXO010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55777_length_1392_cov_3.452506"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /gene="tsaB"
                     /locus_tag="PLH57_11355"
     CDS             complement(<1..357)
                     /gene="tsaB"
                     /locus_tag="PLH57_11355"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRK03252.1"
                     /translation="MLILALDASQIRGVVVLCDERGTVFAQNAGSTSVVHSEGLLTLL
                     HETLENNNYSLKDVDLFACGVGPGSFTGIRVALATVKALAQALGKPMQGVSSLKALAP
                     CSPGDNRRPLMPVYGNV"
     gene            complement(363..>1392)
                     /gene="rseP"
                     /locus_tag="PLH57_11360"
     CDS             complement(363..>1392)
                     /gene="rseP"
                     /locus_tag="PLH57_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRK03253.1"
                     /translation="GISDVNSAAAKQGLKTGDEILTINGKLVRSFEEIEAKVNQLVQS
                     GAKELVFGVVAHEAGFWSPEKLPSAQTEKTVDYKFPAKKLNELGIHSSELFVSSVIKG
                     SPAEIAGVAAGDRLVSIEGKTLHSFSELRTRLQEFGESKSADLTLAIERIVDGKTLRF
                     QKSLTPSVSESKDAAGKETKQYLIGVYPLYIQAEPDFVVERVLNPLTLTAQAWLRALD
                     LSGKTVVSLKKLFFREVSVGTLGGPIMIGKLAGDSMSRGISQFLKAMALISISLAIFN
                     MLPVPILDGGHIFLLGVEVIRGKPISMKQTEFVQQIGLSLIVLLLVVVLFNDFTKVGI
                     PAIRNMFQ"
BASE COUNT          364 a          358 c          321 g          349 t
ORIGIN      
        1 gacgttgcca tagaccggca tcagcggccg gcggttgtcg ccaggcgagc agggcgcgag
       61 cgctttaagc gaagaaaccc cttgcatggg ttttccaaga gcctgcgcca gtgcctttac
      121 ggtggccagc gctactcgaa tacctgtaaa agagccgggc cctacgccac acgcgaaaag
      181 atcgacatct tttagggaat agttattgtt ttcgagagtc tcgtgtaaca gcgttagcaa
      241 gccttcgcta tgaaccacgg aggttgaacc cgcgttctgg gcaaaaacag tgccgcgctc
      301 atcgcaaaga acgactacgc cacgaatttg agaggcatca agtgccagga ttagcatagc
      361 gcctattgaa acatgtttct aatcgcaggt attcccactt tggtgaaatc gttaaataac
      421 accaccacga gtaacaacac gatcaacgaa agaccaatct gctgcacaaa ctctgtctgt
      481 ttcatagaaa tgggtttgcc gcgaatgact tctacgccta gtaaaaaaat atgaccaccg
      541 tccaagatgg gcaccggtaa catgttgaag attgctaagc ttatactaat taaagccatt
      601 gccttcaaaa actgcgatat tccgcgggac atcgaatcac cggcgagttt gccgatcatt
      661 attggcccac ctaaggtacc aaccgacacc tctctgaaaa atagtttctt caacgagacg
      721 acggttttgc ccgaaagatc cagcgctcga agccaagctt gggcagttag cgttaagggg
      781 tttaagaccc gttcgacaac aaagtccggc tcggcctgaa tgtacaaagg atacactccg
      841 atgagatatt gttttgtctc ttttcctgct gcgtccttac tttcgctcac cgagggcgtg
      901 agactttttt ggaatcgcaa agtttttccg tcgacgatgc gctcgattgc gagtgtcaaa
      961 tcagcagact ttgattctcc gaactcttgc aaccgcgtgc gcaactctga aaacgaatgt
     1021 agcgttttac cttcaataga caccagtcga tcgcccgctg ccacgcccgc gatttcagct
     1081 ggggaacctt tgataacgga agatacaaac agttcagaag agtgaatacc taactcgttt
     1141 aacttttttg ccggaaattt atagtcgaca gtcttttcgg tttgcgcgga aggtaatttt
     1201 tctggcgacc aaaaccccgc ctcatgagcg acaacaccga agaccagttc cttcgccccg
     1261 ctttgaacga gctgattcac tttggcttct atttcttcaa agctacgaac gagtttgcca
     1321 tttatggtaa gaatttcgtc gccagttttt agcccctgtt ttgcagctgc tgaattgaca
     1381 tctgaaatcc cg
//
LOCUS       DAOMXO010000665         1390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_55881_length_1390_cov_4.403745, whole genome shotgun sequence.
ACCESSION   DAOMXO010000665 DAOMXO010000000
VERSION     DAOMXO010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55881_length_1390_cov_4.403745"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLH57_11365"
     CDS             <1..195
                     /locus_tag="PLH57_11365"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HRK03254.1"
                     /translation="FFNEFVVEVNDLDRRFQAALNSGLIPGVRLSKFGGSRNELLLAF
                     TEVHSRSDIESLALALRGSN"
     gene            197..>1390
                     /gene="gcvPB"
                     /locus_tag="PLH57_11370"
     CDS             197..>1390
                     /gene="gcvPB"
                     /locus_tag="PLH57_11370"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRK03255.1"
                     /translation="MAITHEEGSLFDIHHPDKSAYKLPPLEVPSKKPTKHLRESTLGL
                     PQVSEMDVVRHYTRLSNMNYSIDAGMYPLGSCTMKYNPKINDEISMLDGFKNIHPYQP
                     LETAAGAIEVMGRLQDLLAEVTGLPAVSLQPAAGAHGEFTGVKVMRAYLKENGGVRRK
                     MLIPDTAHGTNPATSTLCQYEVVRVPNGPNGYIEASDVERLMDRDTAGIMVTNPNTLG
                     LFEKNIVEICKVVHAQGGLVYCDGANFNAVMGWSRPGDLGIDVMHLNLHKTFSTPHGG
                     GGPGAGPICAVEKLRPYLPSPQVVQKDGAYALEYNLPKTIGRVRMAYGNFLVLVRALV
                     YGLTLGRDGLKEASTIAVVNANYILNLLKADYHLPFGPTCLHECVFSDKNQNEFGVKT
                     MEIAKR"
BASE COUNT          368 a          317 c          335 g          370 t
ORIGIN      
        1 ttctttaatg aatttgtcgt cgaagtgaac gatttggatc gccgatttca ggcagcactc
       61 aattccggtc tgatccctgg agttcggctt tcaaaatttg gtggatcacg caatgagctc
      121 ttattagcat tcaccgaagt tcattctcga tccgacattg agtcgttagc tcttgctctg
      181 agaggttcaa attaatatgg ccatcactca cgaagaaggc tctctctttg acattcacca
      241 ccctgataag tcagcttaca aacttcctcc gttagaagtg ccgagtaaaa agcccactaa
      301 acatttgcga gaatcgacgc ttggattgcc acaggtgagc gaaatggatg tcgtccgcca
      361 ttatactcgc ctatcaaaca tgaattacag tatcgacgcg ggcatgtacc ccctcgggtc
      421 ttgcacaatg aaatacaacc ctaaaatcaa cgatgaaatt tcaatgctcg acgggtttaa
      481 aaatattcat ccgtatcagc ctctcgaaac agcagcgggt gccattgaag tgatgggtcg
      541 acttcaagat ttgttggcgg aagttacggg tttacctgct gtgtctttac agccggctgc
      601 gggtgcacac ggtgagttta ctggcgtaaa ggtaatgcgg gcctacttaa aagaaaacgg
      661 tggcgttcgt cggaaaatgc ttattccaga taccgctcac ggtacaaatc ctgccacttc
      721 cacactttgc caatacgaag tagtacgcgt gcctaacggt ccgaatgggt atattgaagc
      781 ttcagatgtt gaacggttaa tggaccgcga tacggccggc atcatggtta ccaatccaaa
      841 cacacttggc ttattcgaaa aaaacattgt cgaaatctgt aaagttgttc atgcccaagg
      901 cggtcttgtt tattgcgacg gtgcaaattt taatgcggtc atgggttggt cgcgaccggg
      961 tgatcttgga atagacgtca tgcacctgaa cctgcataaa actttttcta cgccccacgg
     1021 tggcgggggc cctggggctg ggccgatttg cgcggtcgaa aaattacgcc cctatttgcc
     1081 ttcgccgcaa gtagtacaaa aggacggggc ttacgcgcta gaatataatc taccaaagac
     1141 gatcggtcgc gtacgaatgg catacggaaa ttttttagtt ttagtgcgtg cgctagttta
     1201 cggattaact ctgggtcgcg acggcctcaa ggaagcgtcg accattgccg tagtaaacgc
     1261 caattacatt ttgaatttgt taaaggcaga ttatcatttg ccgttcgggc caacctgcct
     1321 tcatgagtgt gtgttttcag ataaaaatca aaatgaattt ggcgtcaaaa caatggagat
     1381 tgcaaaaagg
//
LOCUS       DAOMXO010000666         1385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_56145_length_1385_cov_4.157895, whole genome shotgun sequence.
ACCESSION   DAOMXO010000666 DAOMXO010000000
VERSION     DAOMXO010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56145_length_1385_cov_4.157895"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLH57_11375"
     CDS             <1..657
                     /locus_tag="PLH57_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRK03256.1"
                     /translation="TRMDRDIGRLMDKLKELDIDKNTLIFFSSDNGTHKEGGADPEFF
                     KSSGPLRGFKRALYDGGIRVPGIAWWPGTLRPGETSDHIWAFWDFLPTACDLAGIATP
                     PNLDGISIMPTLAKHGAPNPTGDQQKLHEYLYWEFHEQGTRQAIRAGNWKGVRLAING
                     PIELYDVVTDPGEAKNLASANPEVVAKIETYLAKARTPHDNWPLKERPQGSSDQLEVV
                     "
     gene            699..775
                     /locus_tag="PLH57_11380"
     tRNA            699..775
                     /locus_tag="PLH57_11380"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:733..735,aa:Met,seq:cat)
     gene            complement(1012..>1385)
                     /locus_tag="PLH57_11385"
     CDS             complement(1012..>1385)
                     /locus_tag="PLH57_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03257.1"
                     /translation="GCAIYPTYPRVPQVTIAGDHALFSAWENGDTGDGAFHRFQGDME
                     PIALYGDYIERILRGMSNLHPLTLQALAVEHPDRVFVSALENGSLAILNYREDGARVR
                     LEGHLDEEMPGYSIRVVGKRD"
BASE COUNT          328 a          446 c          340 g          271 t
ORIGIN      
        1 acccgcatgg atcgcgatat cggccgcctc atggataaac tgaaagaact cgacatcgac
       61 aagaacacgc tcatcttttt ctccagcgac aacggcaccc acaaagaagg cggggccgat
      121 ccggagttct tcaagagctc gggcccgctc cgcggattca agcgcgccct gtacgacggc
      181 ggcatacgcg tccccggcat cgcatggtgg cccggcacgc ttcgcccagg tgaaacctca
      241 gaccacattt gggccttctg ggattttctg cccaccgcat gcgaccttgc aggcatcgca
      301 acgccaccga acctcgatgg catatccatc atgccgacgc tggcaaaaca cggcgcgccc
      361 aaccccaccg gcgatcaaca aaaactacac gaatacctct actgggaatt ccacgaacaa
      421 ggcacccgcc aggcgattcg cgccggcaac tggaaaggcg tgcgcctcgc catcaacggc
      481 cccatcgaac tctatgacgt cgtcaccgac cccggcgaag ccaaaaacct cgcctctgcc
      541 aatcccgaag tagtagcaaa aatagaaacc taccttgcca aagcacgcac cccgcacgac
      601 aactggccct tgaaagaacg cccccaaggc tcaagcgatc agctcgaggt tgtttgacat
      661 ccccatattc aattgctaaa ctaccccttc gccattgacg cggggtggag cagcctggta
      721 gctcgttggg ctcataaccc aaaggtcgta ggttcgaatc ctacccccgc taccatttaa
      781 ttccaagggc ttacgaggaa tcgtgagtcc ttttctcttt tcttcggatc taccttaatt
      841 ctcgcaccga ttgatccatt ccggagctga ctgctcagaa gtctctgcct agaaggtcga
      901 cttctgacag cttgcgattc gatcatcacg ggttatcaac gtcagtttat agacttcgat
      961 cttgaatcga attacaaagt gacgcagcca aagaacaagc attgcaagca actaatcgcg
     1021 cttacccacc acccgaatcg aatatcccgg catctcttcg tcgagatgcc cctccagccg
     1081 cactcgtgcg ccgtcttccc ggtagttcag gatcgccaac gacccatttt cgagcgcgct
     1141 gacgaagact cggtcggggt gttcgaccgc aagggcctgc agggtcaacg ggtgtaagtt
     1201 actcatgcca cgcagaatgc gctcgatgta gtcaccataa agcgcgatgg gttccatgtc
     1261 gccttgaaat cggtggaatg cgccatcgcc cgtgtcgccg ttctcccacg cactgaacag
     1321 ggcatggtcg ccggcgatgg ttacctgcgg gacacgagga taggtgggat agatcgcgca
     1381 cccgc
//
LOCUS       DAOMXO010000667         1385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_56146_length_1385_cov_3.990977, whole genome shotgun sequence.
ACCESSION   DAOMXO010000667 DAOMXO010000000
VERSION     DAOMXO010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56146_length_1385_cov_3.990977"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /gene="trmD"
                     /locus_tag="PLH57_11390"
     CDS             complement(<1..1287)
                     /gene="trmD"
                     /locus_tag="PLH57_11390"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF000648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRK03258.1"
                     /translation="MQISVLTLFPEFFDSPMKTSILGRAFENGTLSLNVHSLRSHGEG
                     PYRSADDRPFGGGSGMVLLPTVIERALGPQGLGLDLEALENDVALYRKRGNAIATNNQ
                     PQEKPFVVALSPQGRRFDAPMAQELASKKNLVFLCGHYEGFDERTLDAFAHLEVSIGD
                     YVLTGGEPAALVMIDAISRFVPGVVGKQESVIGDTFGGSSASDESTEIVPGGLKHAQY
                     TRPNVWRGREVPEVLLSGHHQKIRQWRLIDSERRTIERRPDLLEQAVEYPAYVALLHS
                     PMLNKAGEEVCTAVTNLDLHDIARSCRTYGIEKYFVVNPEPEQETLVGRILGHWHGEI
                     SKVYHPARAEALSGVEFKREFSEVVRSISEGHGGVKPFVVMPDARDLRSKYPAAIPGG
                     WSEKPWTYEELRSRLREHRGAGTDRRVRAIASGQQRF"
BASE COUNT          313 a          398 c          348 g          326 t
ORIGIN      
        1 gaaacgctgc tgcccggatg cgatcgcccg tacgcgacga tccgtaccgg cgccgcggtg
       61 ttcacgaagt cgtgatcgta actcttcgta agtccatggt ttttcactcc agccgccggg
      121 tatcgccgcg gggtatttgc ttctcagatc tcgcgcatcg ggcattacga caaacggttt
      181 tactccccca tggccctcgg agattgaacg caccacttca gaaaattcac gtttaaactc
      241 cacccccgac agggcttcgg ctcgcgctgg gtgataaacc ttcgaaattt ccccatgcca
      301 gtgtccgaga atacgcccca ccaaggtttc ttgttcaggc tcggggttca caacaaaata
      361 cttttcgatt ccataggtac gacagcttcg cgcaatatcg tggagatcga gattggtcac
      421 cgcagtacac acttcttcgc ccgccttgtt caacataggt gagtgcaata gtgcgacgta
      481 agccgggtac tccaccgctt gttccagcaa gtccggtcgg cgttcaattg ttcgccgttc
      541 gctgtcgata aggcgccact gtcggatttt ttgatgatgt cccgagagca gcacctcagg
      601 aacttcgcgc ccccgccata cgtttgggcg tgtgtattgg gcgtgtttta acccccctgg
      661 cactatttct gtactttcgt cggacgcaga cgaaccgccg aaagtatcac cgataacgct
      721 ttcttgtttg ccaacgacac ctggtacgaa ccggcttatc gcgtcgatca tcacaagagc
      781 agcgggctcc cccccagtca gcacataatc cccgatcgac acttcgagat gagcgaacgc
      841 gtctaatgtg cgttcgtcga acccttcata atgaccgcaa agaaacacta ggtttttttt
      901 cgacgcgagc tcttgagcca ttggtgcatc gaagcgtcgc ccctgaggcg agagcgccac
      961 gacaaatggt ttttcttggg gctggttgtt cgtcgcaatc gcgttgccgc gctttcgata
     1021 aagcgccacg tcgttctcaa gagcctcaag gtctaaacca agcccctggg gaccaagcgc
     1081 acgttcgatc accgtaggca gtaaaaccat gccgctgccg ccgccaaagg ggcgatcgtc
     1141 ggccgaacga taagggcctt caccgtggga tcgtagagaa tgtacattca acgacaatgt
     1201 gccgttttca aacgctcgac ctaggatcga ggtcttcatg ggtgagtcga aaaactccgg
     1261 gaacaacgtt aaaactgaga tttgcactac aaacttcttt aagaggggaa gggtttactc
     1321 caaaatttca agaaccgaac gttttcttag tttagtactc gcggcactca gaatcgtgcg
     1381 cacag
//
LOCUS       DAOMXO010000668         1383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_56256_length_1383_cov_5.541416, whole genome shotgun sequence.
ACCESSION   DAOMXO010000668 DAOMXO010000000
VERSION     DAOMXO010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56256_length_1383_cov_5.541416"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLH57_11395"
     CDS             <1..527
                     /locus_tag="PLH57_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRK03259.1"
                     /translation="KVSPVNSARVAKVFDNLWALTKVAIAVCLIYLLASAIATLRRHF
                     MQRHPNALVLEKLYADFAKGDFQGVLDACAENITFQVSGKSRLAGKYTKANFVSDFAA
                     RLRELSGNTLKLEVHDILAGDLHAAVLMTSRLERSGKTIEYRTVHVWRFDGNKPLAWY
                     EYPRDLYHFDQIWN"
     gene            735..>1383
                     /locus_tag="PLH57_11400"
     CDS             735..>1383
                     /locus_tag="PLH57_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HRK03260.1"
                     /translation="MPVLPFLIGALCFMAIGYRYYSAFIAAKVLALDSSRTTPAHTHY
                     DGHNYHPTNKWVLFGHHFAAISGAGPLIGPVLAAQFGFLPGLLWLVIGVVIGGAVHDF
                     VILTASVRHGGRSIAEIARREIGTVAGGVTSLSILFIVVIALAGLGLAVVNALHESAW
                     GTFTISMSVPLALAMGIYIYKMRGGSDSAIKRGSAVGVALLLFAVVAGKWVGDSPT"
BASE COUNT          290 a          387 c          358 g          348 t
ORIGIN      
        1 tgaaagtgag tccggtaaat agcgcgcgcg tcgcaaaagt tttcgacaat ttatgggcac
       61 taactaaggt agccatagcg gtttgcttga tttacctgct cgcctctgcg atagcaactc
      121 tacggaggca ctttatgcaa cgacatccca acgccctagt actcgagaaa ctctacgccg
      181 acttcgcaaa aggcgacttt cagggcgtac tcgacgcttg cgccgagaac attacctttc
      241 aagtctctgg caagagccga ctggccggca agtacaccaa agccaacttc gtttctgact
      301 tcgcggcccg gttgcgcgaa ctctcgggaa acacgttaaa actcgaagta cacgacattt
      361 tagcaggtga tctgcacgcc gccgttctaa tgacgagccg gctcgagcgc agcggtaaga
      421 cgatcgaata ccgcacggtg catgtatggc gctttgacgg caacaagccg ctagcctggt
      481 acgaataccc tcgtgatctg taccatttcg accagatctg gaattagtgc acgcgctccc
      541 agcgcccccg cgcctagtcc cgcggataac aattatggtt gtcaccacag cgcatacaaa
      601 cgtggcgttt gcatgttgag cattggctcg tggccggttc gcgtttacag aaatagcaga
      661 tttggctcat tgaaaattcc ccctaaatac ttgataacac ttttgattag gattttgtaa
      721 gggggacttc ggttatgcct gttctaccat ttttaatcgg cgcactttgc tttatggcca
      781 tcgggtatcg ttattactcg gcatttatcg cggcaaaagt attggcgcta gacagtagcc
      841 gaacgacccc ggcccacacc cactacgacg gtcacaacta tcaccctacg aacaaatggg
      901 tgctcttcgg gcaccacttt gctgccattt ccggcgccgg tcctctcatt ggtccggtgc
      961 tcgccgccca atttgggttt ctccccggcc tcttgtggct cgtcatcggc gtggtgatcg
     1021 gtggtgccgt tcacgacttc gtgattctca ccgccagcgt gcgccatggt ggccgcagca
     1081 ttgcggaaat cgcccgccgc gaaattggta cggtcgcagg tggagtcacc agtctttcga
     1141 ttttatttat tgtcgtcatc gctctcgcag gactcggtct tgcggtcgtc aacgcacttc
     1201 atgaaagcgc ctggggtacg tttacgattt caatgagtgt tccccttgcg ctcgccatgg
     1261 gaatttatat ttacaaaatg cgtggcggct ccgactccgc catcaaacgt ggcagtgccg
     1321 tcggcgtggc attgttattg ttcgccgtgg ttgctggcaa atgggtggga gactcgccga
     1381 ctt
//
LOCUS       DAOMXO010000669         1380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_56429_length_1380_cov_5.895849, whole genome shotgun sequence.
ACCESSION   DAOMXO010000669 DAOMXO010000000
VERSION     DAOMXO010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56429_length_1380_cov_5.895849"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLH57_11405"
     CDS             complement(<1..714)
                     /locus_tag="PLH57_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03261.1"
                     /translation="MNWGNLPRLLGVAIVLVVFQPIHADGGNNDACNLADYLLNHQSE
                     IHQRNAYSVDGSFQDYREQFGVRFVNLTNSLRAPHSWLDVGGGMALPMLQNQSDLLKK
                     LSPEELPTNISLSIETPPLKAEAQTLRNSLPKSAVQYVARGKGLEDFSVDELEALGLQ
                     TLITDRGGGTTYTLRPKDLYQRYHQLLKPKGLLMVSVPPRTKINSGAKTFLAWLRSIK
                     GFKLLAEEPGSYILQRTDEA"
     gene            complement(745..>1380)
                     /locus_tag="PLH57_11410"
     CDS             complement(745..>1380)
                     /locus_tag="PLH57_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03262.1"
                     /translation="HCSAHFETDNGELRIVETKNIGKEIVSTISGALKLGTNSIPTEF
                     ECRYEVEIDSAESIDLGVQIVRGSLTAENWKKNIEVELDWGDVDVGNVGAVNINCGRC
                     TVAGENVSGRFDFRIANGNVGVIGLQNAVVGETLGDLVLKWKNLPHNAKVQVKSRAGD
                     VVLGLPKGVSVALDLKTPKGEVYNSAKIPTYSGGVPFDVAAEIGNIRLYRY"
BASE COUNT          330 a          352 c          323 g          375 t
ORIGIN      
        1 cgcctcgtcg gttcgttgta aaatatagga accaggttcc tcggccaata atttaaatcc
       61 tttaatggat cgtaaccacg ctaaaaaggt tttcgcaccg ctatttattt tggttctcgg
      121 cggcacgctc accatcaata ggcccttggg ttttaatagt tgatgataac gttggtaaag
      181 atccttcgga cgtagcgtgt aggttgtgcc gccgccccga tcggtgatca gcgtttgcaa
      241 acccaacgcc tcgagctcgt cgacactaaa gtcctcgagg ccctttccac gtgccacata
      301 ctgcaccgca gattttggga gtgaattgcg caaggtttgg gcctcagctt ttaggggcgg
      361 tgtttcgatt gagagcgaaa tattggtagg cagttcttcg ggcgatagtt tctttaaaag
      421 atcactttga ttctgcagca tcggaagcgc catgccacct cctacgtcaa gccacgagtg
      481 gggcgctctc aatgagttcg ttagattcac gaaacgtacg ccaaactgtt cacggtaatc
      541 ttgaaacgaa ccgtctactg aataggcgtt gcgttggtgg atttcgcttt gatgattcaa
      601 gagatagtcg gccaggttgc atgcgtcgtt gttccctcca tcagcatgga tgggctgaaa
      661 gacgacaaga acaatcgcta cccctagcag tctaggaaga tttccccaat tcacgggggt
      721 attgtacctg aagggcaaca gtatttaata gcggtacaac cgaatattgc cgatctcagc
      781 ggcgacgtcg aagggaacgc cgcctgaata ggttgggatc tttgcactgt tgtagacttc
      841 gcctttgggg gttttaaggt caagcgcgac cgaaacgccc tttggtagtc ccaacaccac
      901 gtcgccggcg cgcgacttca cttgaacttt ggcattgtga ggcaaatttt tccattttaa
      961 caccagatcg cccaaggttt cgccaactac agcgttttgc aatccaatga cgccgacatt
     1021 gccgttggcg attctaaagt cgaaacggcc actcacgttc tctcccgcca cggtgcagcg
     1081 cccacaattt atattcacgg cacctacgtt gccgacatcg acgtcgcccc aatcgagttc
     1141 aacttctata ttttttttcc aattttcggc agtcaaacta ccccgcacaa tttgaacacc
     1201 caaatctatg gattccgcag aatcaatttc tacttcgtag cgacactcga attccgttgg
     1261 aatagaattg gtaccgagtt tcagagcacc agaaatcgta ctcacgattt cttttccaat
     1321 attttttgtt tcgacaattc gtaattcacc attgtcggtt tcgaaatggg cgctacagtg
//
LOCUS       DAOMXO010000670         1374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_56803_length_1374_cov_4.237301, whole genome shotgun sequence.
ACCESSION   DAOMXO010000670 DAOMXO010000000
VERSION     DAOMXO010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56803_length_1374_cov_4.237301"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLH57_11415"
     CDS             complement(<1..535)
                     /locus_tag="PLH57_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03263.1"
                     /translation="MQSGPIPSGVIHESIEKTSAGLNCRVEIRRKEGAGQYQEFLSAM
                     AHDDAQITPKYFNLRRTSTNTGSTEIIDGKLDPKAGMRVRITGTKNLSIPPRKIPTGT
                     ILECYLPTWFKKNYTRLAPGSKHTIAVFAENAQTRQFQATTANVSVASKQTRKSCLTL
                     TLKFQGVESIWCIKQNGV"
     gene            complement(635..>1374)
                     /locus_tag="PLH57_11420"
     CDS             complement(635..>1374)
                     /locus_tag="PLH57_11420"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRK03264.1"
                     /translation="KAEISTLAGSLAYTTVFSIVPFLAILYVIFERLGGLKYALEKAQ
                     PILINMLAEGVGDTARHTLQRFVDNAHTSSVGWFGFAALLFTTTATYTKISESFRKIW
                     QIQNKYPLFKRLVRLITLLILAPVLITASITLTTAVAAQVKFVPWSGQMAAFAITYIL
                     FVAFYLLVPNTKIPFGVLLRGAFLPAFFWELTKSGYTLYTTRVVQYANLYGSLAAIPL
                     FLLWVYLAWYISLFGAVWIRVLQKSRK"
BASE COUNT          378 a          309 c          329 g          358 t
ORIGIN      
        1 aaacaccatt ttgtttaata caccaaatcg attccacgcc ctgaaatttc aaagtaagtg
       61 ttaaacaact ttttcgggtc tgtttactgg ccaccgaaac attagcagtc gtggcttgaa
      121 actgtcgggt ctgggcattt tccgcaaaga ccgcaatggt gtgctttgag ccaggtgcca
      181 agcgggtgta atttttttta aaccaggtcg gaaggtaaca ctctagaatt gtaccggtcg
      241 ggatttttcg cgggggaata gaaaggtttt tggttccagt aattctgacg cgcatacccg
      301 ctttggggtc tagttttccg tcgatgattt cagtgctccc tgtgttggtg ctggtgcggc
      361 gcaaattaaa atatttaggt gttatttgtg catcatcatg agccattgca cttagaaatt
      421 cttgatattg gccggcgcct tcttttcgtc tgatttcgac tctacaattc aaaccggccg
      481 aagttttttc aatggattca tgtatgacgc ctgaaggaat aggtccagac tgcatggcgt
      541 accattcgtc gtgggccaga cccggaaggg tcagtagtaa aagagtgact ggtattgtta
      601 atcgaaaatg tttcgtaaaa attccttccg gcttttactt gcgcgacttc tgcaaaactc
      661 gaatccagac agcgccaaac agactaatat accaagctaa atagacccag agcaaaaaaa
      721 gtgggatcgc cgccagggag ccataaaggt ttgcatactg tacaaccctg gttgtgtaca
      781 aagtataacc tgatttcgtc agttcccaaa aaaacgctgg caaaaacgcg ccgcgcaata
      841 ggacgccgaa tggaatttta gtattcggca ccaaaaggta gaacgcgacg aacaggatgt
      901 aagtaatagc aaaagctgcc atctgccccg accacggcac gaattttacc tgagcagcca
      961 ccgcggtcgt cagagtgata gaagcggtta ttaacaccgg cgccaaaatc aggagtgtga
     1021 ttaagcgcac gaggcgctta aataggggat acttgttttg tatctgccaa atttttcgaa
     1081 acgattctga gattttggtg taagtcgcgg tcgtcgtaaa taacaacgcg gcaaaaccaa
     1141 accagcccac gctcgatgta tgggcgttgt ccacaaatcg ttgcaaagtg tgccgagcgg
     1201 tgtcaccgac accctctgcc aacatattga ttaagattgg ttgagccttt tccaacgcgt
     1261 atttgagacc acctaggcgc tcaaaaatta cgtagagtat tgccaaaaat ggcacaatcg
     1321 agaacaccgt cgtgtaggct aaacttcccg cgagggtaga aatttccgcc tttt
//
LOCUS       DAOMXO010000671         1374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_56824_length_1374_cov_3.081122, whole genome shotgun sequence.
ACCESSION   DAOMXO010000671 DAOMXO010000000
VERSION     DAOMXO010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56824_length_1374_cov_3.081122"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLH57_11425"
     CDS             <1..196
                     /locus_tag="PLH57_11425"
                     /inference="COORDINATES: protein motif:HMM:NF025539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YaaC family protein"
                     /protein_id="HRK03265.1"
                     /translation="VLASMHRLSELARYRPTLLSRHFELDQNWLLSEFIRCSPQEFID
                     QIACEMTNQDFMFRGHRGKQ"
     gene            193..555
                     /locus_tag="PLH57_11430"
     CDS             193..555
                     /locus_tag="PLH57_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03266.1"
                     /translation="MSKKQIEEQLRKVLKESGVSVGEFRRIVGKVMVEYEQEIGWPET
                     DKGKPWTDAELRVVLSDAPTKENCVKHAKAFGRGYGALEQIYRWAATSDPEIKRLRPN
                     DKFIAQIKRTAKELGWRA"
     gene            842..>1374
                     /locus_tag="PLH57_11435"
     CDS             842..>1374
                     /locus_tag="PLH57_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013069364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03267.1"
                     /translation="MCNAWNHRPTCRCGWGGEGHLGGGRGGATAFALGPSPVMLSGRS
                     AIRNGKQTISFLDPNARCPVCRAKVYFYQSPHGGRVFFDDLGWPWPKHPCTDNQARRL
                     SSDPVQWIGNDNDQLAHFLVHEWVEKGYFPLICTMKKGGKQVGGEFLFTREGESARSV
                     WLFLDSRVHVTKDALFF"
BASE COUNT          337 a          349 c          371 g          317 t
ORIGIN      
        1 tgtgctagct tctatgcatc ggcttagtga acttgcccgc tatcgtccga cactcctaag
       61 tagacatttc gaactggatc aaaactggct cctgtccgaa tttatcagat gttcgccgca
      121 ggaatttatt gaccagatcg cctgtgaaat gacgaaccag gactttatgt ttcgcggaca
      181 tcgaggtaaa caatgagcaa gaaacaaatt gaagaacagt tgcgaaaggt gctgaaggaa
      241 tccggtgtta gtgttggtga gtttcggcgg atagttggca aggtgatggt cgaatatgag
      301 caggagatcg gctggccgga gacggataaa ggcaagccgt ggaccgatgc cgaactacgc
      361 gtggtcttga gcgatgcccc gacaaaagag aactgcgtaa agcacgccaa agccttcggc
      421 cgcggctacg gagcactcga acaaatatac cgctgggccg ccacgagcga cccggaaata
      481 aaacgcctcc gcccaaatga caaattcatc gcccaaatta aacgcaccgc caaagaacta
      541 ggctggaggg cataatatga agctttttat caagcctttc ttactcacgg gaataaaaca
      601 gcacgaaaca gccccaactt accaccacga agttcaactg cgccggagtg gaaccattaa
      661 cggcaagaag gttgtattcg ttcgagcccc gatcgagagg cggagcttca gggtctcttt
      721 cgaatgggag cagatatggt acttcgtcga ttatcaacgg gatctttata agctggtcga
      781 gatcggttcc gataatagtc cttttctcga tttttacgtc gattgattag atcactttca
      841 gatgtgtaat gcgtggaatc atcgtcctac gtgccgttgc ggctggggcg gcgaaggcca
      901 tcttggcggc ggccggggcg gcgcgacggc attcgcactc gggccatcgc cagtcatgct
      961 gtccggtcgg tcggcgatcc gaaatggcaa gcaaaccata agttttctcg atcccaacgc
     1021 gcgctgtccc gtctgtaggg cgaaagtcta cttctaccaa tcaccgcatg ggggccgggt
     1081 attcttcgac gatctcggat ggccttggcc taagcatccc tgcaccgata accaagctcg
     1141 acgtctgtct tcagaccccg tacaatggat cggcaatgat aatgaccagc tcgcccactt
     1201 tctcgtacac gagtgggtag agaaggggta ttttccgctc atttgcacca tgaagaaggg
     1261 tgggaaacag gtcgggggcg agtttctttt tactcgtgag ggcgaatcgg cccgctcggt
     1321 ttggcttttt ctcgactcac gcgttcacgt caccaaagat gctctctttt tcat
//
LOCUS       DAOMXO010000672         1373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_56855_length_1373_cov_4.836874, whole genome shotgun sequence.
ACCESSION   DAOMXO010000672 DAOMXO010000000
VERSION     DAOMXO010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56855_length_1373_cov_4.836874"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLH57_11440"
     CDS             complement(<1..753)
                     /locus_tag="PLH57_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03268.1"
                     /translation="MFLGYHTLFWLALVFAIGTLRPTATTASVVPASRTDAPNCSVLF
                     SEIGLLKQYGEITILPADDQRELFRRFLAGDREAGNQFALQFVRFAAWRARSFQRSFP
                     AFTFEELFLAGWLAIGREMGLRRYNPDLGTPMSYFGAPVNWAIIAHIRTRKRIEGKYI
                     HSKFLESTEQIHEQAETTGALPLERRSHESPFTSEHTQNSTNWERTRDLLDAKTFIQK
                     FRATLKDPKKIFILDDYLLGEMERAEFLKKHNM"
     gene            complement(791..>1373)
                     /locus_tag="PLH57_11445"
     CDS             complement(791..>1373)
                     /locus_tag="PLH57_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRK03269.1"
                     /translation="GLEKVDRGSIKVLGRDVANATDLSRIRGKVGFVFQGFNLFSHLT
                     ILQNVMLAPIRVLKLKRAEAERLALQLLSRVGIAEHAGKYPSQISGGQQQRAAIARTL
                     ALQPEIVLFDEPTSALDPEMTAEVLDVLRELAKSGLTMLVVTHEMGFAREVASRIVFF
                     DQGKIVADDQPRAFFENSENPRLKLFLSRILKH"
BASE COUNT          338 a          338 c          353 g          344 t
ORIGIN      
        1 catgttgtgt ttttttagga actccgcgcg ttccatttcg cctagtaagt aatcgtcgag
       61 aataaatatt tttttcggat ccttgagcgt ggcgcgaaat ttttgaataa acgttttagc
      121 gtcgagtaag tctctcgtac gctcccagtt ggtactgttt tgtgtgtgct ccgaagtgaa
      181 cggcgactcg tgagaccttc gctcgagcgg aagggcgcca gtcgtttccg cctgctcatg
      241 aatctgttca gtcgattcaa ggaatttcga atgaatgtat tttccttcga ttcgtttgcg
      301 agtgcgaata tgtgcaatta tggcccagtt aacgggagct ccgaaataac tcattggcgt
      361 tcccaggtcg ggattgtagc gccttagacc catttcgcga ccaatggcga gccatccagc
      421 tagaaaaagt tcctcaaacg taaaggctgg gaaacttctt tgaaaagacc gcgctcgcca
      481 ggcggcgaaa cgaacgaact gaagggcaaa ctgattgccc gcctcgcgat cacccgcgag
      541 gaagcgtcga aaaagttctc gctgatcatc ggcggggagt atggtgattt cgccatattg
      601 ttttaacagc ccgatttccg aaaataagac gctgcagttg ggagcatcag tccgactggc
      661 gggcacaaca gaggcggtag ttgcagttgg tcgcagggtg ccaattgcga aaaccaacgc
      721 cagccaaaaa agtgtgtggt agcccagaaa catgggattt tattagcata ccttttaaat
      781 caggtaaagg ttagtgcttt agaattcggc taaggaatag ctttagtcgc gggttttcgg
      841 agttttcgaa aaacgccctc ggttggtcgt cggccacgat cttgccttgg tcgaaaaaca
      901 cgattcggct cgccacttca cgagcaaaac ccatttcatg ggttacaacc aacatggtta
      961 gtcccgactt cgccagctcc cgcaaaacgt ccagtacttc ggccgtcatt tcggggtcga
     1021 gcgcgctcgt gggttcgtca aacagtacga tctcgggctg cagcgccaaa gtgcgggcaa
     1081 tcgccgcgcg ctgttgttgc ccaccagaaa tctgagaagg gtatttgccc gcgtgttcag
     1141 caatgcctac acggcttaag agctgaagcg ccagtcgttc ggcttctgca cgttttaact
     1201 ttaaaactcg aatgggcgcc aacatcacat tttgaagaat cgtaagatgg gaaaataaat
     1261 taaatccctg aaatacgaaa cccaccttgc cgcgaatgcg agacaaatca gtcgcgttcg
     1321 ccacgtcgcg acctaaaact tttatggatc ctcggtcgac tttttcaagc ccg
//
LOCUS       DAOMXO010000673         1372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_56913_length_1372_cov_4.637054, whole genome shotgun sequence.
ACCESSION   DAOMXO010000673 DAOMXO010000000
VERSION     DAOMXO010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56913_length_1372_cov_4.637054"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PLH57_11450"
     CDS             <1..1195
                     /locus_tag="PLH57_11450"
                     /inference="COORDINATES: protein motif:HMM:NF019626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HRK03270.1"
                     /translation="IKWAPSFDEELRKSKFKDIPKVDADYYESLPLGFDPEKKITEFE
                     EIIADAKSQVGVEDELARMMIQNCEEYKNVVHMLENRGTKKFYDYAKILYGSPKETFR
                     DEKTTINDLARVMYDILSILDDAVLGPVYPEDLSAEDVIKKLNKRFENYFVDNPVRAK
                     LSDGIVADAAAGSDSVKIREGALFSQRDVDILEVHEGWVHVGTTLNGQNQHICRFLSK
                     GPPRVAPTQEGLAILMEIFSFVSYPRRARTINDRVLGIDKCEDGANILELLEFYRTEG
                     YSEEDCINNAKRVFRGGVLTGGAPFTKDIAYIKGFIENYNFMRAAMKAGKPEYIPYLF
                     VGKIHSSDVPVWYHKHQEGIVDPPKYLPPQFKDLNGVAVWMSFSNVFNMIDMKKVQEY
                     FSKIF"
BASE COUNT          408 a          311 c          330 g          323 t
ORIGIN      
        1 cataaaatgg gctccgtcgt tcgatgaaga acttcgtaaa tctaagttta aagacattcc
       61 aaaagtcgat gccgactact acgagagcct tccgctaggg ttcgatcctg aaaagaaaat
      121 cacggaattt gaagaaatta tagccgatgc taaatcccaa gttggtgttg aagatgaact
      181 ggcacgcatg atgattcaaa actgtgaaga atacaaaaat gtagttcaca tgctcgaaaa
      241 tcgaggcaca aaaaagttct atgattacgc aaaaatactc tatggctctc caaaagagac
      301 ttttcgagat gaaaaaacca ccatcaatga tttagcgcgc gtgatgtacg atattctttc
      361 gattcttgac gacgccgtgc tcgggccggt ttatcccgaa gatctttcag ccgaagatgt
      421 tatcaaaaaa ctaaacaaac gatttgaaaa ctatttcgtc gataaccccg tacgcgccaa
      481 actgtctgac ggaatagtgg cggacgccgc ggcaggcagc gatagcgtga aaattcgaga
      541 gggtgcgctt ttttcacagc gtgacgtcga catcctagaa gtacacgaag gttgggtgca
      601 tgtgggcacc acgctcaacg gtcagaatca gcacatttgt cgctttttat ctaaggggcc
      661 gccgcgggtg gcgccaaccc aagaagggct agcgatctta atggagattt tttcgttcgt
      721 aagttacccg cgacgtgcgc gcactataaa tgaccgagtg cttggtatcg acaagtgcga
      781 agacggcgca aacattctgg aactgctcga gttctaccgg acagagggat actccgaaga
      841 agactgcata aataacgcaa agcgcgtatt tcggggtggg gtcctaaccg gtggcgctcc
      901 ttttacaaaa gacattgcct atatcaaggg attcattgag aattacaact tcatgagggc
      961 agcgatgaag gccggaaaac cggaatacat cccttacctc tttgtcggaa aaatacactc
     1021 ctcggacgtg ccggtttggt accataaaca ccaagagggg atcgtcgatc cgccgaaata
     1081 cctgccgccg cagttcaaag acttgaacgg tgtggcagtt tggatgagct tttcgaacgt
     1141 gtttaacatg atcgacatga agaaggttca ggaatacttt tcaaagattt tttaatcgag
     1201 acaggaggcc tttatgaaaa ccaagattct atttacccta gcgacgatgc taattgcgtc
     1261 tgctcccgcg ttcgccgcga agattgccac ggtcggtgaa aaaaccatta acactgaaga
     1321 tctcaaagcc gaatattccc aaatttccga cgaacaacgt gcggcgatca at
//
LOCUS       DAOMXO010000674         1370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_57031_length_1370_cov_4.558175, whole genome shotgun sequence.
ACCESSION   DAOMXO010000674 DAOMXO010000000
VERSION     DAOMXO010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57031_length_1370_cov_4.558175"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLH57_11455"
     CDS             complement(<1..450)
                     /locus_tag="PLH57_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HRK03271.1"
                     /translation="MGLRVNTNIASLNSQRHLRLTRAALDKSLEKLSSGSRINRAGDD
                     AAGLAISENLRAQIKGLQQAERNALDGVSLVQVAEGGLQEISNILIRLRELAVQAASD
                     TVGPTERKFLNREYDALLQEVDRIANATEYNRISLLNGSGTVFDIQVG"
     gene            complement(587..754)
                     /locus_tag="PLH57_11460"
     CDS             complement(587..754)
                     /locus_tag="PLH57_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03272.1"
                     /translation="MAITVLTRKAGAVKEIVYRYWAGNGLSLDNLNYQVDRIILGDEE
                     FQDFINDVIFK"
     gene            781..>1370
                     /locus_tag="PLH57_11465"
     CDS             781..>1370
                     /locus_tag="PLH57_11465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK03273.1"
                     /translation="MPNRNGFFCRVLPAAVCGTIVEMSFEIRNPTSQDLDALETLLNA
                     SFQYDANESYCVDFAPLFSNTTLESSRIITKNGTIAASGALHVSRVKVGSESFKAGII
                     GAIATAPQHRNAGLASQVLAELEGLALRHNCETLVLWSDQVAFYEKLGFKAAGTQEIY
                     LLTELFAAINEKNLDRILNETKGSAAYGWNEEVKAL"
BASE COUNT          343 a          346 c          318 g          363 t
ORIGIN      
        1 accgacctga atatcgaaca ccgtaccgct accattaagt agagagatgc ggttgtactc
       61 ggtcgcgttc gcaatacgat cgacctcttg taagagagca tcgtattcgc gattgagaaa
      121 ttttctttcg gttggcccca cggtatcgga agctgcctgc actgcaagtt cgcgcagccg
      181 gatgaggatg ttgctgattt cctgcaatcc gccttctgcg acctgcacca agctcacacc
      241 gtcgagtgcg tttctttctg cctgttgtag gcccttaatt tgggctctta ggttttcgga
      301 gattgcgagt cccgcggcgt catcaccggc gcgattgatt cggctgcctg acgagagttt
      361 ttccaaactc ttatcgagag cggcgcgcgt caaacgcaag tggcgctgcg aattaagcga
      421 agcaatgttt gtattgaccc tgagacccat aatttcctcc ttgaaacgtc taaatcaagc
      481 gacttcccgt cactcacaac acgtatcggc ggcagttttt gggactttag gaaaaacaca
      541 tgtccatgtg acactgctta atatgaagcg gcaatctttg ccgggcctac ttaaagatca
      601 cgtcattaat gaaatcctga aattcttcat cgccaagaat aatgcggtct acttggtagt
      661 ttaaattatc gagcgaaagc ccgtttccag cccaataacg atatacgatt tcttttactg
      721 cccctgcttt acgagtaagt accgtaatcg ccataccttc gttgctcgca ggggcaaaac
      781 ttgccgaacc gaaatggttt tttctgccga gtgttgccag ctgccgtttg tggaacaatt
      841 gttgagatga gttttgaaat tcgaaatcca acaagccaag acctcgatgc gctggaaaca
      901 ctcttaaatg cgagtttcca gtacgatgcc aacgaaagtt actgtgtcga ttttgctccg
      961 cttttttcta acaccaccct agagtcgtct cgcatcatta ctaagaatgg cacaatcgca
     1021 gcttctggcg ctttgcacgt tagccgcgtt aaagttggat ccgagtcctt taaagccggt
     1081 attattggag caatcgccac cgcacctcaa caccgcaacg ctggtcttgc gtcacaggtg
     1141 ttggccgaac tcgaggggtt agcactaaga cacaattgtg aaacgcttgt actctggagc
     1201 gatcaggtgg cattttacga aaaacttggc tttaaggctg ctggcactca ggaaatttat
     1261 ttgctcaccg aacttttcgc cgccatcaac gaaaaaaatc ttgatcgcat tctcaacgaa
     1321 accaaaggaa gcgcggcgta tggctggaac gaagaagtta aagccctata
//
LOCUS       DAOMXO010000675         1369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_57084_length_1369_cov_4.312024, whole genome shotgun sequence.
ACCESSION   DAOMXO010000675 DAOMXO010000000
VERSION     DAOMXO010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57084_length_1369_cov_4.312024"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /gene="ribA"
                     /locus_tag="PLH57_11470"
     CDS             <1..721
                     /gene="ribA"
                     /locus_tag="PLH57_11470"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRK03274.1"
                     /translation="RMPELKVFAQTHNIKLVSVRDLIAYRMQRESLVEKISDAKLPTM
                     DAGEFRIFGFKSVIDGAEHVALVKGSLENLSVTEGKAPLVRVHSECVTGDAFGSARCD
                     CGPQLQAALKMIDREGSGALIYMRSQEGRGIGLLNKIKAYALQDTGMDTVEANTHLGF
                     KADLRHYGIGAQILKQLGIRRLRLLTNNPKKVVGLDGYDLEITERVAIEVEANPANQA
                     YLAAKRDKLGHLLEQPLNGLH"
     gene            723..1151
                     /gene="ribH"
                     /locus_tag="PLH57_11475"
     CDS             723..1151
                     /gene="ribH"
                     /locus_tag="PLH57_11475"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRK03275.1"
                     /translation="MRIALVVASFNPEITGGLETGARKYLNEQGISDAQIERYDSPGA
                     FEIPLIAKKLASTGRFAGVVCLGAVIKGDTMHFEFISLGATIGILNASLQTDTPISFG
                     VLTTITEEQANVRSRDDAHNKGREAAAACIASVRVLQSIQ"
BASE COUNT          370 a          343 c          332 g          324 t
ORIGIN      
        1 ccgcatgccg gaactaaaag ttttcgctca aactcataac atcaagctcg taagtgtacg
       61 cgacctgatc gcctatcgca tgcagcgtga gtcgctggtc gaaaaaatca gcgatgcaaa
      121 actgcctacg atggatgcgg gcgaatttag aatttttggt ttcaaaagtg tcatcgatgg
      181 tgctgaacac gtagctctcg ttaaaggatc tctcgaaaat ctgagcgtaa ctgagggcaa
      241 agcaccgcta gtgcgggtcc actcggaatg cgttactggt gatgcattcg gttctgcccg
      301 ctgcgactgt ggcccgcaac tacaagctgc tttaaaaatg attgaccgtg aaggtagtgg
      361 tgcattgatt tacatgcgct cacaagaggg gcgcggcatc ggattattaa acaagatcaa
      421 agcctatgcg ttgcaagaca ctggtatgga caccgtggag gccaacaccc acctgggatt
      481 taaggcagac cttcgccact acggcatcgg cgcccaaata ttaaaacaac tcggaattcg
      541 ccgtttgcgc ttactgacga acaaccctaa aaaagttgtg ggacttgatg gatatgactt
      601 ggaaatcact gaacgcgtgg ccatcgaggt tgaagccaac ccggccaacc aagcgtatct
      661 cgctgctaaa cgcgataaac ttggtcacct gctcgaacaa cccttgaacg gactacacta
      721 aaatgcgcat cgcactcgta gttgcaagtt ttaaccccga aatcacaggc ggtctcgaaa
      781 ccggcgctcg aaaatattta aacgaacaag gtatttcgga cgcccagatt gaacgctacg
      841 attccccagg agctttcgaa attccattga tcgccaaaaa attagcaagc actgggcgtt
      901 ttgctggagt ggtatgcttg ggcgccgtga ttaagggcga tactatgcac tttgaattca
      961 ttagcctcgg cgcgaccatt ggaattttaa atgcgtcgct ccaaactgac acgccaatat
     1021 cgtttggcgt acttactaca atcacggaag aacaggccaa tgttcgctcc agggatgacg
     1081 cgcacaacaa aggccgcgaa gcagctgcgg catgcattgc ctcagttcga gtgttacaaa
     1141 gcatccaata acatgttttt ttggagggct gcgcgtgcgc tacgctttct tgattttggc
     1201 tgtttttcta gttcaccaca cgggtcaggc ctcgcagcta gaaactttac tcgccgaagt
     1261 atgtaaaact cattctaaag accctcgtaa atttcaaata ctttcaacgc cggtgcgaca
     1321 tctccccgtg cgagagcaat gttttcggcc aaatgtaaaa gcgcgcgat
//
LOCUS       DAOMXO010000676         1368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_57132_length_1368_cov_4.381569, whole genome shotgun sequence.
ACCESSION   DAOMXO010000676 DAOMXO010000000
VERSION     DAOMXO010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57132_length_1368_cov_4.381569"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLH57_11480"
     CDS             <1..226
                     /locus_tag="PLH57_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03276.1"
                     /translation="SVFVEIRKRLPDETKSSELCSLTGKVLEAENLTDSEMRVVVNVV
                     QVDELSWNALLNIFDSRQAQISEFLNAVRG"
     gene            231..>1368
                     /locus_tag="PLH57_11485"
     CDS             231..>1368
                     /locus_tag="PLH57_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03277.1"
                     /translation="MSATPEKESDIFKKYLATRKVLIADPSATSRSGLFRVFQDCGSP
                     PNSVILVNSYGQAKETIKNIKPHIVITEFDIDRRSGSGLDLLIEQRQQQPKETKECLF
                     VIATNNTSQSAVARAAEEDVDAFIIKPFTVETVRKTLVKAALLKLKPPEYLLLVEKGK
                     EALIAQNYDEAEKLLEQAVPLDPAPSLALYYIGMTKFARQITEEAEGKYNKGLDFNKI
                     HYKCLVGLYELMGSQKRHHEAYEVVKRVSQYFPANPKRLSEVLRLAIITQQYDDVEKY
                     YQAFCNIDERDDTLVKYVCAALVVCGKYYLGTGARSRAMTLFQKASATGTGRANIIKE
                     IVQALVEANLPKDARDYQKKFPPEFQTSKDYQLLNFEITNLEGPV"
BASE COUNT          428 a          319 c          302 g          319 t
ORIGIN      
        1 cagcgtcttc gtagaaatca gaaaaagact tcccgacgaa acaaaatcat cggaactgtg
       61 ttctttgacg ggaaaagtac tcgaagcaga aaacctaaca gattccgaaa tgcgtgtagt
      121 ggtcaacgtg gttcaggtag atgaacttag ctggaacgcc ttgctcaata tttttgattc
      181 acgccaagct caaatcagtg aatttttaaa cgctgtaaga ggataaatca gtgtctgcaa
      241 ctccagaaaa agaatctgac atttttaaaa aatatctcgc tactagaaag gtgctgatcg
      301 cggatcccag cgccacctca cgttctgggt tgtttagagt ttttcaagat tgcggttcgc
      361 cgcccaattc agtgattctc gtaaattcct acggacaagc aaaagaaacg atcaaaaata
      421 taaagcccca catcgtaatt accgaatttg atatcgatcg acgttctgga agtgggctcg
      481 acctgcttat tgaacaaagg cagcaacagc ccaaagaaac gaaggagtgc cttttcgtaa
      541 tcgcgaccaa caacacttcg cagtcagccg ttgcgagagc cgccgaagaa gatgtcgatg
      601 cttttataat taaacccttt actgttgaaa ccgttcgaaa aactctcgta aaggccgcgc
      661 tactcaaact aaaaccaccc gagtatttgc tgctcgtaga aaagggaaag gaagcgttga
      721 tcgcgcaaaa ctacgatgag gctgagaaac ttctggaaca agccgtgcca cttgatcccg
      781 ccccatctct tgcactttac tacataggta tgaccaagtt cgcacggcaa atcactgagg
      841 aggctgaggg caaatacaat aagggtctcg acttcaacaa aattcactac aaatgccttg
      901 taggtctcta cgaattaatg ggttcccaaa agcggcacca cgaagcctat gaggttgtga
      961 aacgagtatc ccaatatttt ccagcaaatc ccaaacgcct ttcggaagta ttgcgtttgg
     1021 cgattatcac gcaacagtac gacgacgtgg aaaagtatta tcaagccttc tgcaatatcg
     1081 acgaacgaga cgacacattg gtgaaatatg tttgtgccgc gcttgtagtt tgcggcaaat
     1141 attacttggg caccggggcc agaagtcgcg ccatgacact ttttcagaaa gcatctgcca
     1201 ctggcacagg cagagcaaac attattaaag agatcgtgca ggcgcttgta gaagcaaact
     1261 tgcccaaaga tgcgcgcgat tatcaaaaaa agtttccgcc ggaatttcaa acaagcaagg
     1321 actaccaact actgaatttc gagatcacca atctcgaggg cccggtat
//
LOCUS       DAOMXO010000677         1353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_58035_length_1353_cov_3.535439, whole genome shotgun sequence.
ACCESSION   DAOMXO010000677 DAOMXO010000000
VERSION     DAOMXO010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58035_length_1353_cov_3.535439"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..822
                     /locus_tag="PLH57_11490"
     CDS             163..822
                     /locus_tag="PLH57_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03278.1"
                     /translation="MPIGSSQKQTDISLMSNEAKIYHIRQKHPIKGGTASVPDKEEIC
                     ESQYKIAKFFWNRASAIVFVEGLTEDLEEKNRKSYLEQNPKLNIYLETFSNGLPSRYE
                     DLNEDQKFLLENNMAAPLLWVLGRLNNVYATGSGSEQRSLYDRGVVLLGECDLNSVAH
                     IDCGKSKEIYEVILKKREELALARVEAFLAKQSATPEEIFIIFGSLHDFGASIKIASG
                     F"
     gene            complement(819..>1353)
                     /locus_tag="PLH57_11495"
     CDS             complement(819..>1353)
                     /locus_tag="PLH57_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK03279.1"
                     /translation="FKKLLAMDPKHTDAAISLSVIYNDIGKYDEAKKIYQVANQSLQL
                     KRKGSDEMLDRKFALKHIELGDLYLKYHRYDDALDDYSRAARLAPADLSIRIKLAKVY
                     AKKGFTTRAIQELQQLCNEHPEYTSARLHLGLMYFSQGNVIDAQLEWEKARNQDPNNP
                     ELQTYLNMASRATETSV"
BASE COUNT          404 a          260 c          324 g          365 t
ORIGIN      
        1 aaaaattaac ccgaacaaga aaagaaccaa aatcggagaa gtgagggtaa agtatccacg
       61 agacatttaa agcaacgtat ttgacctttt aaattaaatc aactttagga gaagccggga
      121 agtcagtagc gaagcgacat atcgttttgg ggtaggttag ttgtgcctat cggaagttct
      181 cagaaacaga ccgatatttc attgatgagt aacgaggcaa aaatctacca tattcgccag
      241 aagcacccga taaaaggcgg gaccgctagt gtccctgata aagaagagat ttgtgagtct
      301 caatataaaa tagcaaagtt tttctggaat cgggccagcg ccatagtttt cgtcgagggg
      361 ctaaccgaag atctcgaaga aaagaaccgc aaaagctact tagagcaaaa tccgaaattg
      421 aatatttatt tagagacatt ttcaaacggg cttcctagcc gttacgaaga cctaaatgag
      481 gatcaaaaat ttcttctaga aaataacatg gccgccccct tgttgtgggt tttgggtcga
      541 ctaaacaatg tgtacgctac tggaagtggt agtgagcaac gttcgttgta tgatcgcgga
      601 gtagttttgt taggcgaatg cgatcttaat tctgttgcac acatagattg cggcaagagc
      661 aaagaaatct atgaagtaat cctaaaaaaa agagaagaac ttgcgctagc tagagtagag
      721 gcgtttctgg cgaaacagag cgctactcca gaagaaatat ttataatttt tgggtcgctc
      781 cacgactttg gtgcaagcat aaaaatcgcc agcggttttt aaacactcgt ctcagtcgca
      841 cgggacgcca tgttcaggta agtctgtagt tcagggttat tcgggtcttg attgcgggcc
      901 ttttcccact cgagttgagc atcaatgaca ttaccctgag aaaaatacat taaaccaagg
      961 tgaaggcgag cagaagtgta ctctgggtgt tcgttgcaga gctgttggag ctcctgtatc
     1021 gcacgtgtag tgaatccttt tttcgcataa accttagcaa gtttaatgcg aatggaaagg
     1081 tcggcgggtg ccaggcgcgc tgctctggaa taatcatcca gtgcatcatc atagcgatga
     1141 tatttaaggt aaagatcacc cagctcaatg tgtttgaggg caaatttgcg atccagcatt
     1201 tcgtcgcttc ccttgcgttt caactgtagc gactgatttg cgacctggta aattttcttg
     1261 gcttcgtcgt atttacctat gtcgttgtaa atgacactca aggaaattgc ggcatctgta
     1321 tgtttaggat ccatagctag caattttttg aat
//
LOCUS       DAOMXO010000678         1346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_58476_length_1346_cov_3.687064, whole genome shotgun sequence.
ACCESSION   DAOMXO010000678 DAOMXO010000000
VERSION     DAOMXO010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58476_length_1346_cov_3.687064"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..>1346
                     /locus_tag="PLH57_11500"
     CDS             91..>1346
                     /locus_tag="PLH57_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03280.1"
                     /translation="MLAKNPRPYAGDIERWVEYLEPKSKELNQQIAGENEIKSRYYQR
                     KDKIAREQKQADLEREVKIAEQKAKEYKAVYERVRRKFDADEVLRAAKKLGIKANKVK
                     DTSDWVVVVDLNRGDVQVFNGLYHFKKSDFFTVRERVVNGVNAIVESNNLVVFSPEEY
                     FSKSGMGFDFYKLGQYLSKGGIKTRDALALAGDELVRRSFGKSDMNLASVPESLYAQI
                     EIDGKMTQVRFTQYNPASRAFIGEKLTLNPDGTVTQSAMRSFYIDNYAGEIAFLRPEE
                     AIPYVQGLEKVEAPNVKPLEEATSIGPPPKIDEDHAQKVVRQNRGMAVREGVKESIGH
                     EVAGEAQVQAVGKSAEVIRRSNIVNVELDPSNPSLKREVTIQNFGNGAIVSYDLPEGT
                     RATVWIEDYKLNSTKNKIVDVAVRKW"
BASE COUNT          426 a          265 c          343 g          312 t
ORIGIN      
        1 gttcaacgct ccccacattt gacgcgttca ttgggtcccg cggaaaattg gtcgattgtt
       61 ccctatagtc agttaaaaag gaaaaaagag ttgttggcga aaaatcctcg cccatacgcc
      121 ggggatatag agcgttgggt agagtacttg gaacctaagt caaaagaatt gaatcagcag
      181 atcgcaggcg aaaatgaaat taaaagccga tattaccaac gcaaagacaa gatcgctaga
      241 gagcaaaagc aggctgatct cgaacgagaa gtgaaaatcg ctgaacaaaa agcgaaagag
      301 tataaggcag tgtacgaacg agtgcgccga aaattcgacg ccgatgaagt tttgagagct
      361 gcaaaaaaac taggaataaa agcgaataag gtaaaagaca cgtctgactg ggtagtggtc
      421 gtagatctaa atcgaggtga tgttcaggtc ttcaatggtc tttatcactt taaaaaatcc
      481 gatttcttta ctgttcgaga acgagtggtt aacggcgtaa acgctatcgt agagtccaac
      541 aatcttgtgg tatttagccc agaggaatat ttttcgaaaa gcgggatggg atttgatttc
      601 tataaactcg gccaatatct atctaagggc ggaattaaaa cccgtgatgc cttggctctg
      661 gcgggtgacg aactggttcg tcgttctttt ggcaaaagtg acatgaacct tgcttcggtg
      721 ccagaaagcc tttatgcgca aattgagatt gacggtaaaa tgacccaagt tcgatttact
      781 cagtacaacc ccgcatctcg agcttttatc ggcgaaaaat tgacactcaa tccagatggg
      841 acagttactc aaagtgcgat gcgttctttt tacattgata actacgcagg tgaaattgcc
      901 tttttacggc cagaagaggc tattccctat gttcaaggtc tggaaaaagt cgaagcgcca
      961 aacgttaaac ccctcgaaga ggcgacatcg atcggacccc ctcccaagat tgacgaggac
     1021 catgcgcaga aagtggtgcg gcaaaatcgt ggtatggcag tgcgagaggg tgtgaaggag
     1081 tccatcgggc acgaagtagc aggcgaggcc caagttcagg ctgttggaaa aagtgcggaa
     1141 gtcatcagac gtagtaatat tgtgaatgtc gaactcgacc caagtaaccc gagcctaaaa
     1201 cgtgaagtga caattcaaaa tttcggaaac ggagccattg tgtcttatga cttgccggag
     1261 ggtacgagag ccacagtttg gattgaagac tacaaattaa attccacgaa aaataaaatc
     1321 gtagatgtag cggttcgaaa atggaa
//
LOCUS       DAOMXO010000679         1345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_58518_length_1345_cov_5.533333, whole genome shotgun sequence.
ACCESSION   DAOMXO010000679 DAOMXO010000000
VERSION     DAOMXO010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58518_length_1345_cov_5.533333"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..>1345
                     /locus_tag="PLH57_11505"
     CDS             144..>1345
                     /locus_tag="PLH57_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03281.1"
                     /translation="MKELLKGLLLPFRMTARQSAVNPNLRSIPPVRKRSNIDHRRSVA
                     QAKAWDPTPPYWPWEHDAILACHQMNIENPLTPPLPNSPGYGPWASEINQYGACVDVG
                     RNAGGQTAAMREFIEYGLNKKIGNQSAIGFIIDLAADFVRTDVQLKHVYDELSDLLIN
                     NKTFQDIVKFHETMVFALHSKYGPHAMSVVQGFLRTLPIVNQTNSTGQDRVQPLLNIA
                     AKVIESPSAPGAAVLVYDLLDEAEKRPAPTIAPKVIPRSFPVNLNNEIVVARAIELLN
                     QVEKTKTDTERQRRLNEMFNEYLGYVKSDERRWEYRNATEFKSSLKPLLDEIAKGSRI
                     EWILAFMHYLESDPYDPKWWEGWFHRLSSRVFPIAYQYPGEAKRVRLVNYLDLLELIV
                     TDANFTLK"
BASE COUNT          374 a          308 c          322 g          341 t
ORIGIN      
        1 cgtcctggtc gtctgcccga cctcgctcaa gcaccaatgg caacgcgaga tcgcccgctt
       61 cagcgagtgc cgtagcgggt cgctcgcaga acgagttaaa aaacgcgttt gcgctcactg
      121 ctaaaatggc gaagaacggt tctttgaagg aattgctcaa aggtttgttg ttgccgtttc
      181 gcatgacggc tcgccaatcg gcggtcaatc cgaatcttcg cagtattcct ccggtgcgaa
      241 agcgatccaa tattgaccat cgtcgcagtg tcgctcaggc caaagcttgg gatccgacgc
      301 ctccttactg gccatgggaa cacgatgcga tattagcgtg ccatcaaatg aatatcgaaa
      361 atcccctaac cccacctctg ccaaattcac ccggctacgg gccttgggct agtgaaataa
      421 accagtacgg ggcgtgcgtt gacgtcggcc gtaatgccgg cggtcaaaca gcggctatga
      481 gggaatttat agagtatggg ttaaataaaa aaattggcaa tcaaagcgcc atcggattca
      541 taattgatct ggccgcagac tttgtaagaa ctgacgtgca gttgaaacat gtctatgacg
      601 agcttagcga tcttttaatt aacaataaaa cgtttcaaga tattgtgaag tttcatgaaa
      661 ccatggtttt tgctcttcac tccaaatatg gacctcatgc catgagtgtg gttcaaggtt
      721 ttttgcgaac tcttccgata gttaatcaaa ccaattcgac tggacaggac cgtgttcaac
      781 cgctgttaaa tatagcagca aaggtgatcg aaagtcctag tgcccctggg gcagcagttt
      841 tagtctatga tttattggat gaggcagaaa aaagacctgc ccctacgatt gcgcccaaag
      901 ttattcctcg aagttttcct gtgaatttaa acaatgagat tgttgtagct agggccatcg
      961 aactcttaaa tcaggtagaa aagaccaaaa ctgataccga gcgacaacgg cgcctaaatg
     1021 aaatgttcaa cgaatatttg ggttacgtaa aaagcgatga gcgacgttgg gaatatcgca
     1081 acgctacaga gtttaaatcc tcactaaagc ccttattaga cgaaatcgcc aagggatccc
     1141 gaatcgaatg gatactagct tttatgcatt atcttgagag tgatccctac gacccgaaat
     1201 ggtgggaggg ctggtttcac cgtttatctt cacgagtgtt tccgatcgcc tatcagtacc
     1261 ctggtgaagc caaaagagtg cggttggtaa attatttaga tctattggag ctgatcgtaa
     1321 ccgacgcgaa ttttacccta aaaga
//
LOCUS       DAOMXO010000680         1344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_58588_length_1344_cov_5.750970, whole genome shotgun sequence.
ACCESSION   DAOMXO010000680 DAOMXO010000000
VERSION     DAOMXO010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58588_length_1344_cov_5.750970"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PLH57_11510"
     CDS             complement(<1..648)
                     /locus_tag="PLH57_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03282.1"
                     /translation="MDFHVLRSHITKMLFVGASLTVVAVCPIPSSVAGQTAHHNARHE
                     AKPSVFRAFFDEMIRKVRELAAADQVNQELRARVAELELMNQRLAYETLKNRESARVE
                     ENRKKNEAEGGRAASRTIASLEPSDAHILSKPPKEIFSAGVQAFGKGDHETAASAFLA
                     LAENKENTAYHRPQVFFLAGVSLFKMKNYKTAKTYFEKAAQSAEALGEQSDELAYA"
     gene            complement(713..1150)
                     /locus_tag="PLH57_11515"
     CDS             complement(713..1150)
                     /locus_tag="PLH57_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03283.1"
                     /translation="MSRQRIVKIGLLLSLATAGILLRGSLNKGFHAIAVATGTEDATD
                     PRVPGEVGTIDRIKARAAEKRKVYSEKFRARRELNARHKAEIEELKTKHRAAREEFAG
                     SDQAKESSSEERKNFFRAQRAEMQELKDRQRKERIALSPESKP"
BASE COUNT          311 a          339 c          297 g          397 t
ORIGIN      
        1 agcataggcc aactcgtcag actgctcacc cagtgcttcg gcactttgag cggctttttc
       61 aaaataagtt ttagcagttt tgtaattttt catcttaaag agacttactc cagccaggaa
      121 aaatacctgt ggccggtgat aagctgtgtt ttctttattt tccgccagtg ccaagaacgc
      181 gctagccgct gtttcgtgat caccttttcc aaaagcttgt actccggcgc taaaaatttc
      241 tttggggggt ttagaaagaa tatgagcatc cgacggttca agcgacgcaa tggtacgcga
      301 tgccgcacgc ccaccctcgg cttcattttt tttgcgattt tcttcgaccc gtgctgactc
      361 tctattttta agtgtttcgt aggctagccg ttggttcatt aattccaact ccgctactcg
      421 ggcacgaagt tcttgattca cttgatctgc ggcggccagc tctcgtactt ttcgtatcat
      481 ttcgtcaaag aaagcacgaa acacactagg ttttgcttca tgacgggcgt tatggtgggc
      541 tgtctgacct gctacggaac taggaattgg acacactgcc acgactgtta agctcgcgcc
      601 aacgaacaac attttagtta tgtgcgaacg caatacgtga aagtccaagg ccttcctcct
      661 cattggtctc tatcgggcga aagtgtctaa agactgaggg gatttggtag gattatggtt
      721 tcgactcggg cgaaagggca attctttcct tacgctgacg gtctttaagt tcctgcattt
      781 cggctcgctg agccctaaag aagttttttc tttcttcgct ggaactttct ttggcttgat
      841 cggaaccggc aaactcttcc ctggcggcgc ggtgttttgt tttcaactct tcgatttccg
      901 ctttatgccg ggcgtttaat tcacgacgag ctcgaaattt ttcggagtaa acttttcttt
      961 tttcggcggc ccgagcttta attcggtcta tcgtcccaac ttcgccgggc acccgtgggt
     1021 cggtcgcatc ttcagtaccc gtggccactg caatcgcgtg gaacccttta ttcaaagatc
     1081 cacgcaacag aatacccgcg gtcgcaagac tcaaaagtaa tccaattttt actattctct
     1141 gccgactcat actgacggca tcatacgacg gacgctcgaa tcaatcaatg tactcgtaac
     1201 gatcaatggc gtagccgaaa aaggaaactg aaaaatccgt aacaaattca accactaccg
     1261 agtcagtatc catgtagtcg gtagtatatc cgttagggtg acttccagat atttcctcta
     1321 aaacctcacc atttgcgtct ttaa
//
LOCUS       DAOMXO010000681         1343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_58663_length_1343_cov_3.944099, whole genome shotgun sequence.
ACCESSION   DAOMXO010000681 DAOMXO010000000
VERSION     DAOMXO010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58663_length_1343_cov_3.944099"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..>1343)
                     /locus_tag="PLH57_11520"
     CDS             complement(67..>1343)
                     /locus_tag="PLH57_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03284.1"
                     /translation="IIPRPHGHAISKLSVLGHEGKTLAYRKDYIVHETGDGLSYLIEF
                     RKKQPNGVRPSVEFVRQPTGPGINSELSSLDLDALAKINARLNKVGFTQIANALDQRI
                     AFARKQGASLSMSDLESIVAHAGLYSYIKESQIDPRAIELDSTFGPHAKFLHQGVLCA
                     QCDGANAFFADIMQEYFKAAGSKLEVFDASSLVNQAGILNDGIRHAQIGIRDPNSSDR
                     IASFLIYDATAKRKDPRNPKSVETPQGDLDSQPIARTRSRVKDTIKELEANDTNLKAT
                     FSKYKHRVMKSASDPIVRTTRLNMIPSDLLGGKITLPDATALLNETLGLTGDDAFASF
                     QSIDEMLSTIQQQARKTLTLIDRLDESKSLRTIQLPVRDAARTTLRTIDAELESINST
                     DVFDHWTGLDRIPDVDGAACSLLLIMQRAKSN"
BASE COUNT          332 a          278 c          349 g          384 t
ORIGIN      
        1 gtcaaattga agcgaaggtt atcgaaaaac ttcgtgcctt ttaccaagac tatattcgtt
       61 aataatttaa ttggatttcg cgcgttgcat tatgagcaac aaagagcagg ccgcgccatc
      121 gacgtcgggt atgcggtcga gtccagtcca gtgatcgaaa acatcggtgc tgttgataga
      181 ttccaactct gcgtcaatgg ttcgaagggt ggttcttgcg gcatcccgga cgggaagttg
      241 aatggtccga agcgatttcg attcgtctag tcgatctatg agagtcagcg tctttcttgc
      301 ttgctgttga attgtcgaaa gcatttcatc aatcgattga aaagaagcaa aagcgtcatc
      361 acccgttagg cccaatgttt cattgagaag tgcggttgcg tcgggcaaag tgatttttcc
      421 tccgagcaaa tctgaaggaa tcatgtttaa tcgagtggtg cgaacaattg ggtcactcgc
      481 ggatttcatt acacggtgtt tatatttaga gaacgtcgct tttagattcg tatcgttcgc
      541 ttcgagttct ttgattgtat cttttacgcg agaccgagtt cgagcaatgg gttgcgagtc
      601 gagatcgccc tggggagtct cgacggattt cggattacga gggtcctttc ttttggctgt
      661 agcatcatag ataagaaacg aagctatacg atcagagctg ttaggatcgc gaataccgat
      721 ctgtgcgtgt cgaataccat catttaagat gcccgcttga tttactaaag acgaagcgtc
      781 gaaaacttcg agtttgctac ccgcggcctt gaagtattct tgcataatat cggcaaaaaa
      841 agcgttcgcg ccgtcgcatt gtgcgcagag caccccttgg tgcaagaact tggcatgggg
      901 gccaaaggtc gagtcgagtt caatggcacg tgggtcgatt tgagattcct ttatgtacga
      961 ataaagtccc gcgtgcgcga ctatagattc aagatcactc atagaaagag aggccccttg
     1021 ttttcgtgcg aacgcgatac gttggtcgag tgcattggcg atttgggtaa agcctacttt
     1081 atttagacga gcattgattt ttgctaacgc atcgagatcg agactactta gttcggagtt
     1141 aatcccggga cctgttggtt gtcttacgaa ctcgacgcta gggcgaacgc cgttaggttg
     1201 ttttttacga aattcgatta agtacgaaag tccgtctccc gtctcatgaa caatatagtc
     1261 ttttcggtag gccagagttt taccttcatg gccaagcacg gatagttttg aaattgcatg
     1321 cccatgggga cgcggaatta tta
//
LOCUS       DAOMXO010000682         1342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_58723_length_1342_cov_4.209013, whole genome shotgun sequence.
ACCESSION   DAOMXO010000682 DAOMXO010000000
VERSION     DAOMXO010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58723_length_1342_cov_4.209013"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..>1342)
                     /locus_tag="PLH57_11525"
     CDS             complement(117..>1342)
                     /locus_tag="PLH57_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03285.1"
                     /translation="HMLVSTGAITLPDYHSAKYVQGRTDEKAAAVHKIGALLIEKSNG
                     DNIFPYVGDVNGEFNLRHVEFIEESQGFYDVGRFYSAKSVEPLRPLAVVLGDIHVGST
                     HQRLLEAFRNQILTLKPREVILHDLMDGYSISHHERDLLITMAKKAETGELDLKAEIE
                     NVISFVNSMLAIDPQLLITIVHSNHNFWLYRWLQDGEYIGDAKNDRIGHLLAVIARQG
                     KDPLETVLKYGLSDFDQATGERKYLIMPVSAEARLAFTHPGESKKVGPRNRRVELALH
                     GHAGAGGRGGSMLAFGRGNSGVARGHTHTVSRVRNDVNIGTFTHLKLGYSMDGMSNWV
                     QALGLIGPHGEKQILVFRDGEFYADPAGAKRAPEEFFVEGYPKLIPNNDAAPGPGDFD
                     QHGGGEPPRRVRRKK"
BASE COUNT          308 a          366 c          337 g          331 t
ORIGIN      
        1 acctacgacc tttgggttat gagcccaacg agctaccagg ctgctccacc ccgcgatcct
       61 attcgtgaag ggccgacaat agagattcgc ggaatcaaag tcaactgcta gtttttttac
      121 tttttacggc gaactcttcg agggggttcg ccgccgccgt gttggtcaaa gtcgcctggg
      181 cctggcgcgg catcgttgtt aggaatgagt ttcggatacc cttcaacgaa gaactcctcc
      241 ggcgcacgtt tggcaccagc tggatccgca taaaattcac cgtcacggaa cactagaatc
      301 tgtttttcac cgtgagggcc aattaagcca agcgcctgta cccagttgct cataccgtcc
      361 atcgaatatc ctagcttgag atgggtaaaa gtaccaatgt tcacgtcgtt gcgtactcgg
      421 cttacggtgt gggtgtgtcc gcgcgcgaca cctgagttac cacgcccgaa tgcgagcatc
      481 gaaccacccc gaccgccagc tcccgcatga ccgtggagcg cgagttcaac cctacggttg
      541 cgagggccaa ccttcttact ctcccccggg tgggtaaagg ccaggcgggc ctcggccgac
      601 actggcatga tcagatactt tcgttcgccg gttgcttgat caaagtcgga aagtccgtac
      661 tttaaaaccg tttccagggg gtctttaccc tgcctggcaa tgacggcgag caaatgtccg
      721 atacgatcat ttttggcgtc gccaatgtat tccccgtcct gcaaccaacg gtagagccag
      781 aaattatgat ttgaatgaac aatagtgatc aatagctgtg ggtcgatggc tagcattgag
      841 ttcacgaacg agatcacatt ttctatttca gctttgaggt caagctcgcc cgtttctgcc
      901 tttttggcca tggttatcaa caaatcacgt tcgtgatggc tgatgctgta cccgtccatc
      961 aagtcgtgca aaatcacttc acgcggcttg agagttaaaa tctgattgcg gaacgcttca
     1021 agcaaacgtt ggtgcgttga gccgacatga atgtcaccca gcacgaccgc gagcgggcgc
     1081 aaaggttcta ccgacttggc gctatagaat cggccgacat catagaaccc ttgggactct
     1141 tcgatgaatt ctacatggcg tagattaaac tctccgttga cgtcgcccac gtaaggaaaa
     1201 atgttatccc cgttactctt ttcaattaag agcgccccaa tcttatgaac agcggctgct
     1261 ttctcgtcag ttcgaccctg aacgtacttt gctgagtgat aatcgggcag ggtgatcgca
     1321 ccagtgctca ccaacatgtg gt
//
LOCUS       DAOMXO010000683         1341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_58795_length_1341_cov_3.753499, whole genome shotgun sequence.
ACCESSION   DAOMXO010000683 DAOMXO010000000
VERSION     DAOMXO010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58795_length_1341_cov_3.753499"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /gene="tig"
                     /locus_tag="PLH57_11530"
     CDS             complement(<1..660)
                     /gene="tig"
                     /locus_tag="PLH57_11530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRK03286.1"
                     /translation="MNYECKVENTSPIKRQLTISISAESVKDYMDRQFDSLQKTAKIK
                     GFRQGKVPVPVLKQYFLSDVKSEVFSKVVRDSYVKALEEHSIVAVGMPEIEAKSNAEL
                     KDGEPVTFVARVEVYPEIKIGDLTKLKVVRQSSEVTDEDVENALSRIRESHAELVTDE
                     RDASGPGAKTGDTLLISFEGTLPDGRKLDSLKAEKRTLELGAGQFMEEFEAGVTGMKV
                     GE"
     gene            complement(703..>1341)
                     /gene="truA"
                     /locus_tag="PLH57_11535"
     CDS             complement(703..>1341)
                     /gene="truA"
                     /locus_tag="PLH57_11535"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRK03287.1"
                     /translation="RVSVFSSGRTDAGVHARVQAVHTNLPEKWEHTEALQSALNSLLP
                     SDIRILKAEIMPDHFHALRDAKKKMYVYFINPQPVQNPVLARYSWHLRFPLDWNAMAQ
                     AGQAFVGRHDFKAFAAADHSAKTTVRTIESFEWGTLVVPGFGGNTGLGYFKVVGQGFL
                     KNMVRIMVGSLVRVGAGKAPVDSIARALISGQRNQAGSTAPPHGLWLWDVLY"
BASE COUNT          318 a          368 c          311 g          344 t
ORIGIN      
        1 ctctccgacc ttcattccag taacacccgc ttcgaattct tccataaact gaccggcacc
       61 taattcgagg gtgcgttttt cagcttttaa gctgtctaat ttgcggccgt ccggcagtgt
      121 accctcgaac gaaatcaaca gcgtatcacc ggttttggcg cccggaccac tggcatcgcg
      181 ttcgtcggtc accaactctg cgtgcgactc acgaatgcgt gacaaagcat tttctacgtc
      241 ttcatcggtt acttcacttg attggcggac aacttttaac ttggtgaggt cgccaatttt
      301 aatttcagga tatacttcaa ctcgcgccac gaaagttacg ggttctccgt ctttgagttc
      361 agcattcgac ttggcctcaa tctcgggcat accaacagct acgatcgagt gttcttcaag
      421 tgccttcaca taactgtcgc gcacgacttt cgaaaatact tcagacttca cgtcgctcaa
      481 gaaatattgt ttcaaaaccg gaacaggcac tttcccttgt cggaagcctt tgatcttcgc
      541 ggttttttga agcgaatcaa actgacggtc catgtagtcc ttaacggact cggccgatat
      601 ggagatggtt aattgccgtt taatcggcga cgtattttcg actttgcatt cataattcat
      661 agacgaacgt tcatatcgac tttagggatt ggggtcaacg ctctagtaaa ggacgtccca
      721 caaccaaagc ccatgggggg gggccgtaga gcccgcctgg tttcgctggc cacttattag
      781 agcccgagca atcgaatcaa cgggagcctt gccggccccc actcgaacca gggaacccac
      841 catgatccgc accatatttt taagaaatcc ctgacctacc accttaaaat agcccaaacc
      901 agtattccca ccaaaccccg ggacaacgag cgtaccccac tcgaaactct cgatggttcg
      961 gaccgtcgtt ttggcactat gatccgcggc ggcgaaggct ttgaaatcgt gtcgtcccac
     1021 aaatgcctgc cctgcttgcg ccatcgcatt ccagtcgaga ggaaatcgta gatgccaact
     1081 gtaacgggcc agcaccgggt tttgaacggg ctggggatta atgaagtaaa cgtacatttt
     1141 ttttttggca tctcgcagag catgaaagtg atcgggcatg atttcggcct tcaagatccg
     1201 tatatcgctc ggcaataagc tgttgagcgc gctttggagc gcctcggtgt gttcccattt
     1261 ttcgggtaaa ttggtatgaa cggcctgaac tcgcgcgtga actccagcat cagttcgccc
     1321 tgaagaaaac accgaaactc g
//
LOCUS       DAOMXO010000684         1336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_59117_length_1336_cov_4.629977, whole genome shotgun sequence.
ACCESSION   DAOMXO010000684 DAOMXO010000000
VERSION     DAOMXO010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59117_length_1336_cov_4.629977"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..591
                     /locus_tag="PLH57_11540"
     CDS             76..591
                     /locus_tag="PLH57_11540"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRK03288.1"
                     /translation="MNNPYFPTKVGHLLVTWFGFGRVPIAPGTAGSLAAVPICWVLAE
                     YVPLASKVVIAIGFTCIAILAAAQECYLKPEIKDPGFIVIDEVAGMLWATIGISTDWP
                     AGWMSILAAFLLFRIFDVVKPYPAKFFDRASKTTPSWFRRGAHIVLDDVIAGFFAAFV
                     VNALIFYGILT"
     gene            725..>1336
                     /locus_tag="PLH57_11545"
     CDS             725..>1336
                     /locus_tag="PLH57_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HRK03289.1"
                     /translation="MSQLNHPNSTSPSQFSKHDEAGAANRSKALELAVSTIEKQFGKG
                     SIMRLGEGQTLVQDVPVLSTGSLSLDLALGIGGLPRGRVIEIFGPESSGKTTLTLHCI
                     AEAQKQGGVAAFVDAEHALDVEYARKLGVRTEDLLISQPDTGEQALEIADMLVRSGAV
                     DLLVVDSVAALVPRAEIEGEMGDSHMGLQARLMSQALRKLTGTI"
BASE COUNT          321 a          330 c          324 g          361 t
ORIGIN      
        1 tagaaatcgc ccctctttgg aatttacgca caccgcgctt gatttgcaat tcccgagcgc
       61 gttagaagta ggaaaatgaa caatccgtat tttcccacaa aagtagggca cctcttggtc
      121 acttggtttg gatttggtcg cgtgcctatt gctccgggca ccgccggtag tttggcggca
      181 gttccaattt gttgggttct cgcggaatat gttcccctcg cttcaaaagt agtaatcgcg
      241 atcggattta cctgcatcgc aattcttgct gcagcgcaag agtgttactt aaaacccgaa
      301 atcaaagacc ctggattcat cgtaatcgac gaagttgcag gtatgctctg ggcaaccatt
      361 ggcatatcca ctgattggcc cgctggttgg atgtccattc tcgcggcatt tctacttttt
      421 cgcattttcg acgtagtcaa accttatccg gcaaaatttt ttgaccgagc gtcgaaaact
      481 acacccagct ggttccgacg tggcgctcat atagtgcttg atgacgtgat cgcaggtttt
      541 tttgccgctt ttgttgtaaa tgctttgatt ttttacggaa tcctcactta acgcatcgcg
      601 attattttta tttattgctc gcgaatcgca actggactgc attaaagaat aatctggcgc
      661 gcgatttgct ggacgcattc ttttagaacg tgttaattca aacaaaaaca aggagacact
      721 caccatgagt cagttaaacc atccgaattc cacatcccca tctcaatttt ctaaacacga
      781 cgaagctggc gccgcaaatc gctcgaaggc tcttgagcta gctgtcagca caattgaaaa
      841 gcaatttggc aaaggctcga ttatgcgctt gggcgaagga caaacgctcg ttcaagacgt
      901 tcctgttttg tcgaccggat ccctaagcct tgacctggca ctcggaattg gcggattgcc
      961 tcgcggaagg gttattgaaa ttttcggacc tgagtcgtct ggtaagacca ctcttaccct
     1021 ccactgtatt gcagaggcgc aaaaacaagg tggcgtagcc gcatttgtcg acgcggaaca
     1081 cgcgctcgac gtagaatacg ctcgaaaact gggtgtgcgc actgaagacc ttttaatttc
     1141 gcagcctgac accggtgaac aagcgctcga aattgctgac atgctggtgc gaagcggtgc
     1201 ggttgatcta ttagtggtcg actccgttgc tgctctcgtg ccgcgcgcag aaatcgaagg
     1261 tgaaatgggc gactcacaca tgggcttgca agcaaggctc atgagccaag ctcttcgtaa
     1321 gttgactggt accatt
//
LOCUS       DAOMXO010000685         1335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_59167_length_1335_cov_4.506250, whole genome shotgun sequence.
ACCESSION   DAOMXO010000685 DAOMXO010000000
VERSION     DAOMXO010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59167_length_1335_cov_4.506250"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLH57_11550"
     CDS             complement(<1..717)
                     /locus_tag="PLH57_11550"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRK03290.1"
                     /translation="MKTAIIKQHGAPNEPGVIMFEERPTPIPNANQVLVRVRACGLNH
                     LDTWVRRGVPGHTFPLPMIPGSDIAGTVEKVGSAVTKFKPGERVIVNPSLSCGHCMQC
                     DSNRHQLCPQWGLLGETSDGGCCEYIAVEQRQLYHLAPSISFEQAACIPVAFVTAWQM
                     LVKKAAIQPGQTILIHGAGSGVSVASIQIAKMFGLQVYVTSSSEEKLSRAKSLGAHRF
                     INTSKESFRDAVRNLTGKQGV"
     gene            735..>1335
                     /locus_tag="PLH57_11555"
     CDS             735..>1335
                     /locus_tag="PLH57_11555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRK03291.1"
                     /translation="MAYEYGKSNWRFDWTFFAICALAASVFLVGVDRAPVPTVDGAVR
                     ASEARTIVETGTWFPIYYQGAEVHDHPPLFVWITALSFKIFGINDFAANFPSRIFAAL
                     TLLFTGLIAIEIGFSRGVALLSVLVLAATRDFVLSNVRGYIEPLLSFFIYGGLWLVFR
                     QRRRRSYWAAVGAGICVFGAFFSKGPPALWPFLAFTILLI"
BASE COUNT          291 a          324 c          331 g          389 t
ORIGIN      
        1 gaccccctgt ttcccagtta aattccgaac agcatcacga aatgattctt tagatgtatt
       61 tataaatcta tgagcaccaa gtgattttgc ccgacttaat ttttcttcgg aactggatgt
      121 aacgtaaact tgtaggccga acattttggc aatttgaatc gacgcaacac taacaccact
      181 tccagcaccg tgaatgagta tagtttgtcc gggctgaatg gcggcttttt ttacgagcat
      241 ttgccaagcc gtcacgaagg ctactggaat acaagctgcc tgttcaaaac taattgaggg
      301 cgctaaatgg taaagctgcc gttgctcgac tgcgatatat tcgcaacacc caccgtcgga
      361 cgtttcacca agtaatcccc actgtggaca aagttggtgc cgatttgaat cgcactgcat
      421 gcaatgcccg cagctaagtg atgggttcac gattacccgc tcacctggtt tgaatttggt
      481 aaccgcggat cctacttttt caactgttcc agcgatatcg ctccctggaa tcatgggtaa
      541 aggaaatgta tgaccgggaa cacctcgccg tacccaggta tcgaggtgat tcagaccaca
      601 cgctcgaacg cgaacgagta cttggttggc attcggtatc ggggtcggcc gctcctcgaa
      661 catgatcacg cctggttcgt ttggtgcgcc gtgctgcttg atgattgccg ttttcatcgt
      721 tagtgtctga taatatggcg tacgaatacg gtaaaagcaa ttggcggttc gactggactt
      781 tttttgcaat ctgcgcgctc gcagcttcag tattcttagt cggagtcgat cgagcgcctg
      841 taccaacggt agacggagcg gtgcgcgcaa gtgaggctcg cacaatcgtg gaaaccggaa
      901 cctggtttcc tatttattat caaggcgccg aagtacacga tcacccacct ttgttcgttt
      961 ggattacggc attgagtttc aagatttttg gaatcaacga tttcgctgcc aattttccga
     1021 gtcgaatttt cgccgctctt acgctgttgt tcacggggct cattgccatt gaaatagggt
     1081 tttctcgcgg cgtcgctttg ctttcagtat tggtactggc ggctacgcga gatttcgttt
     1141 taagcaatgt tcggggttac atcgagccac tacttagttt ttttatttac ggaggcctct
     1201 ggttagtgtt ccgccagcgt cgtcgccgca gctactgggc ggctgttggg gctggcattt
     1261 gcgtattcgg agcttttttt tccaaaggac cgcccgcact ctggcccttt ttggcgttta
     1321 cgatcttatt aatat
//
LOCUS       DAOMXO010000686         1333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_59309_length_1333_cov_4.298905, whole genome shotgun sequence.
ACCESSION   DAOMXO010000686 DAOMXO010000000
VERSION     DAOMXO010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59309_length_1333_cov_4.298905"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..871)
                     /gene="lipB"
                     /locus_tag="PLH57_11560"
     CDS             complement(95..871)
                     /gene="lipB"
                     /locus_tag="PLH57_11560"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRK03292.1"
                     /translation="MQTLDLGRKNYLEAWAIQKEFVEKRAAGEIEDTLLFVEHEPVYT
                     VGRASQSSLDRSGESADGLASDRITDSIPPATETASIHVRKLGDVPVVEVERGGKLTF
                     HGPGQIVGYPIFHLTRRDLRGYLREMERILMSTISEQALLSVKPCPETLFLEPGQLQT
                     GVWVRDKKLASIGIAVRKWVSFHGFALNVHTDLRYFEAVEPCGFSGSIMTSIERELGG
                     MGDMRVLVSNLKRTLAEKFSVLSNTYHAAPLQSPEVSASV"
     gene            complement(891..>1333)
                     /locus_tag="PLH57_11565"
     CDS             complement(891..>1333)
                     /locus_tag="PLH57_11565"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988102.1"
                     /note="E3 component of pyruvate complex; catalyzes the
                     oxidation of dihydrolipoamide to lipoamide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRK03293.1"
                     /translation="NIAGHKAAFDTTIIPAVVFTDPEIAAVGLTLAEALKQGFDAHEA
                     SFPFAALGKALATGESDGFAKLVADRKTQRLLGVHVVGANASTILGEAGLALEMGAVL
                     EDLALTIHAHPTLPEALMEAAEVALGHPIHIVAKPPRASAEKSA"
BASE COUNT          307 a          368 c          329 g          329 t
ORIGIN      
        1 cttttattta ccctaagatt tttgctcggt aagacgctga caatctagga ccgcaccttc
       61 gagtctcaag ctcaactgct ctgatttttc taagtcaaac cgacgcagaa acctctggcg
      121 attgcagcgg tgctgcgtga taggtgttgg acagtactga aaacttttca gccaaggtgc
      181 gtttgaggtt gctcaccagt actcgcatgt cgcccatacc accaagttcg cgttcaatcg
      241 acgtcatgat ggacccactg aaaccgcagg gttcgaccgc ttcaaaataa cgtaaatcgg
      301 tgtgcacgtt gagcgcaaag ccgtgaaagc ttacccattt gcgcacggca atgccaatgc
      361 tggctaattt tttgtcgcgc acccaaacac cggtttgcag ctggccgggt tctaaaaaca
      421 aagtttctgg gcaaggcttc actgatagca gcgcctgttc agaaatggtg ctcatgagaa
      481 ttcgttccat ttcgcgcaaa tacccacgta ggtcacgtcg agtaagatga aaaatcgggt
      541 accctacaat ttgaccaggt ccgtgaaaag tcagtttacc accacgttcg acctcgacta
      601 ccggaacatc gcctagtttt cgcacatgaa tcgaagcggt ttctgttgcc ggcggaatag
      661 aatccgtgat tcggtccgaa gcaagaccat cggcgctttc gccagaccga tcgagagaag
      721 actgcgacgc acgcccaacc gtatacaccg gttcgtgctc cacaaataaa agtgtgtctt
      781 caatttctcc ggccgcgcgt ttttcgacga attctttttg aatcgcccac gcttcgaggt
      841 aattttttcg gccgagatcg agagtttgca taagtccttt attatttacg ttaagccgac
      901 ttttcggcgg aggcccttgg tggtttagca actatgtgga tcggatgtcc gagcgccact
      961 tccgccgctt ccatcaaggc ttccggcaac gtcggatgcg cgtgaattgt aagtgccagg
     1021 tcttctagca ccgcgcccat ttcaagcgca agccctgctt cgcccaagat tgtgctcgca
     1081 ttagcgccca ccacatgcac tccgagtagc cgctgggttt tacggtcggc cactagtttc
     1141 gcgaatccgt cagactcgcc ggtagccagc gccttgccaa gcgcagcgaa cggaaagctt
     1201 gcctcgtgtg cgtcgaagcc ctgcttcaac gcttcggcta aagttaaccc aacggccgcg
     1261 atttcgggat cggtaaaaac tacagctgga ataatggtgg tatcgaacgc cgccttgtgc
     1321 ccggcaatgt ttt
//
LOCUS       DAOMXO010000687         1332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_59403_length_1332_cov_2.537980, whole genome shotgun sequence.
ACCESSION   DAOMXO010000687 DAOMXO010000000
VERSION     DAOMXO010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59403_length_1332_cov_2.537980"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PLH57_11570"
     CDS             complement(<1..1322)
                     /locus_tag="PLH57_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRK03294.1"
                     /translation="MKYLRGEYHSAFNDFKKLATVKNKKKYRSLSKERLNYWMGMALY
                     REGKFDKSKIYFERLAADRNKGYYSVAATKRLEKLDIELKGQEKIATTTVKEEITGSN
                     LVNHFRKGFDSRMVASIKEAPIEEEVAAEAQEAEEEADPMEMDKPDEKVAEASEESPF
                     KTPDFVEKFEKSRALATLGLDFWSKWELYEIEKRTKNKEYLKVLLNEYSQVGQYHRMS
                     HISQVYFGGQKTRLGIEQGRGLWEYSFPKAYPTLVDRYSKEFEVPRELVWSIMKAESQ
                     YKKEAISPVGALGLMQVMPYTGNRVAEILGDKTFTPKQLLEPDSAIKIGTKYLQRLNR
                     KFNQAIPLVAASYNAGPHRVKNWLQQFGNLETDEFIEHIPFLETRNYVKKVIANSYIY
                     SELYGERKEKKSPDLVPYLAEKIPFSYNADSGDGDDVKGDQRTHRRDE"
BASE COUNT          310 a          339 c          247 g          436 t
ORIGIN      
        1 tcctcgtcgc gccgatgtgt gcgctggtcg cccttgacgt cgtccccgtc gccactgtcc
       61 gcattgtaac taaacggaat tttctctgct agataaggaa ctaaatccgg agacttcttt
      121 tcttttcgtt caccgtataa ctctgaatag atataactat tcgcgatgac ttttttgacg
      181 tagttacgcg tctccaaaaa cgggatgtgc tcaataaact catccgtctc tagattaccg
      241 aattgctgta accagttctt aactcggtgt ggccctgcat tgtaactggc cgctaccaga
      301 gggatcgctt ggttaaattt tcgatttaat cgttgtaaat acttggtgcc gatcttaatc
      361 gctgaatctg gctctagaag ctgtttaggc gtgaatgttt tatctccaag gatttcggct
      421 acgcggtttc ctgtataggg catcacctgc attaaaccca atgcaccaac cggcgaaatc
      481 gcttctttct tgtactgact ctcggccttc ataatgctcc aaactaactc acgcggaact
      541 tcaaactctt tcgaatatcg atcgacaagt gtgggatagg ctttcgggaa cgaatactcc
      601 cagagtcctc gaccttgttc aatacctaaa cgagtcttct gcccaccgaa gtacacttgg
      661 ctaatatggc tcatgcgatg gtactgccct acctgcgagt attcgtttag tagcactttc
      721 aagtactctt tgtttttagt acgcttttca atttcgtaca attcccactt cgaccagaaa
      781 tccaatccca acgtcgcaag cgcgcgagac ttttcaaact tttctacgaa atctggggtt
      841 ttaaacggac tttcttcact tgcttcagcg accttttcgt ccggtttatc catttccatt
      901 ggatccgctt cttcttcagc ctcttgagct tcggcagcta cctcttcttc gattggtgct
      961 tctttgatgg aagctaccat tctagaatcg aaacctttac ggaagtgatt cactagattc
     1021 gaacccgtga tttcctcttt aaccgtcgtt gttgcaattt tctcttggcc ttttagctct
     1081 atatctaatt tttctaaacg ctttgtcgcg gctacagagt aataaccttt gttccgatca
     1141 gccgctaaac gttcaaagta aattttcgat ttatcaaact taccctcgcg atacaaggcc
     1201 attcccatcc agtaattcaa acgctctttg cttaatgaac ggtatttctt tttgtttttc
     1261 accgttgcca attttttaaa atcattaaat gccgaatgat attcgccacg caagtatttc
     1321 atccacgcta gg
//
LOCUS       DAOMXO010000688         1325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_59790_length_1325_cov_6.542520, whole genome shotgun sequence.
ACCESSION   DAOMXO010000688 DAOMXO010000000
VERSION     DAOMXO010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59790_length_1325_cov_6.542520"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PLH57_11575"
     CDS             complement(<1..761)
                     /locus_tag="PLH57_11575"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRK03295.1"
                     /translation="MKICQNHPMQSLRKVLIANRGEIAIRIARTARKMGLKTVAVYSE
                     LDRRSPHLDYCDEAFYLGNGPISDTYLAVPKLIEALQKHGAGAVHPGYGFLSERAHFV
                     RAVEAAGGIFVGPRAESMEAMGDKIQAKILMKKMGVPLLPGTDGGATTLSEVKKNAAR
                     IGYPILLKAAAGGGGKGMRIVRTEKELPDALEAASKEAQRYFGDGTVYLEKYLENPHH
                     VEVQVLGDGRGGGGHVFDRECSIQRRHQKLVEESPA"
     gene            complement(812..>1325)
                     /locus_tag="PLH57_11580"
     CDS             complement(812..>1325)
                     /locus_tag="PLH57_11580"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRK03296.1"
                     /translation="YILDFVMVSYFLLPQVRITYFDSRVRWWENHPRYWMSSAVKLQL
                     MERVVEAEVDNVSVGGAYLLTKETFEIGDCVRLQFKVFSNEYDVNAEIVHKGKGSGGY
                     GARFVHTPETLEAFERLTRAMEILRIPRRPDYKNSWEGFLDWARTLLKTGRGIVPEVP
                     EAFIHRPKQN"
BASE COUNT          317 a          367 c          305 g          336 t
ORIGIN      
        1 gctggacttt cttctaccag tttctggtgt cggcgctgta tcgaacactc acgatcaaag
       61 acatgaccac ctccaccccg tccatcaccc aatacctgga cctcgacgtg gtgcggattc
      121 tctaaatatt tttccaggta aactgtgccg tctccaaaat agcgttgagc ttccttggat
      181 gctgcctcta aggcatcggg caactctttt tcggtgcgaa cgatccgcat tccttttccg
      241 ccaccgccag ccgcagcttt gagtaaaatt gggtaaccta tacgtgcagc gttttttttg
      301 acttcagaca aagtcgtcgc accaccgtct gttccaggaa gcaacggcac gcccattttt
      361 ttcatgagta tttttgcctg aattttatcg cccattgctt ccatgctctc agcacgaggt
      421 cctacgaata ttccgccagc ggcctcgacg gcgcgaacaa agtgcgcccg ttctgagagg
      481 aacccgtacc cagggtgaac tgcgcccgcg ccatgtttct gcaaagcttc tatcaattta
      541 gggaccgcga ggtaggtatc agatattgga ccattgccca ggtaaaaggc ttcgtcacaa
      601 taatcgagat ggggactacg acgatcgagc tctgaataca ccgcaacagt cttcagtccc
      661 atttttcggg cagttcgcgc aattcgaata gcgatttcgc cacggttagc gatcaggact
      721 ttacgtagtg actgcattgg gtggttttga caaatcttca cgggagatgc aagcgccgag
      781 gctaatacgg ctgcttactc ccgcgaagat atcagttttg cttcggacga tgaataaatg
      841 cctcgggtac ctctggtaca atgccacgcc ccgttttaag cagggttcta gcccaatcaa
      901 gaaacccttc ccaggagttc ttgtaatcgg gccgccttgg aattctgagg atctccatcg
      961 ctcgggtgag ccgctcaaag gcctctagcg tctctggggt atgtacaaat cgagcgccgt
     1021 acccgccgga ccccttcccc ttgtgaacaa tctccgcgtt gacgtcgtat tcgttgctaa
     1081 aaaccttaaa ctgaagacgt acgcaatcac cgatttcaaa cgtttcttta gttaagagat
     1141 aggcgccacc cacgctcaca ttgtcgactt cggcttctac cacacgctcc ataagttgaa
     1201 gttttacagc cgacgacatc caatatcgcg ggtgattttc ccaccaacgc actcgggaat
     1261 cgaagtaagt aattcgaact tggggcaata aaaagtaaga caccattaca aagtcgagaa
     1321 tgtag
//
LOCUS       DAOMXO010000689         1325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_59791_length_1325_cov_5.620472, whole genome shotgun sequence.
ACCESSION   DAOMXO010000689 DAOMXO010000000
VERSION     DAOMXO010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59791_length_1325_cov_5.620472"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PLH57_11585"
     CDS             complement(<1..1161)
                     /locus_tag="PLH57_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03297.1"
                     /translation="MHTDRSFFEVITPNQLEDVWEQVRGDQPWGDRVRVLVGPHGSRT
                     VIKEWLLERVPGVASGSVTGVEEFAREILMRAGAPLAVPLSRAAQIDALEVISKDPNF
                     RAGDSKLARALRTRSIAKRLAAYVTRFDYAWSDLQQLEATLEAVSEREGQRFESQKKL
                     LEALVLLWKRGVFWPWSGAELLRQASERVLEAFGEPAKHNSPIEVYLWGFCEWTEVEQ
                     AFFRRLSESENIRVHQVLPSQGKSGVSYLWRPHSEFDELENLTDELLKLRDEGGTLKS
                     TAVIAPKKNIGRRRLLEHLHRAGVAAREVSPTESWRQSASAHRWRNVWHALAGQSSID
                     LVLPLLDRRAKTHGLLTKSLASGQVKGQRALKKLFEETSDERLRTMVALSRSL"
BASE COUNT          308 a          403 c          315 g          299 t
ORIGIN      
        1 cagactccgc gacagtgcaa ccatggtacg tagccgctcg tcggaagttt cctcaaacaa
       61 tttctttagt gcgcgctggc cctttacttg tccggaggct agagacttcg tcaacaagcc
      121 gtgggttttg gctcgacgat ccaacagcgg caacaccaga tcgatactcg actggccagc
      181 aagcgcatgc caaacgttgc gccaccgatg cgccgaagca ctctgtcgcc aagattccgt
      241 gggagacact tcccgcgcgg cgactccagc ccgatgcaaa tgctcgagca aacgacgccg
      301 cccaatattt tttttcggcg caatcactgc tgtactcttt aaagtacctc cttcgtcccg
      361 cagtttcagc aactcgtcgg ttaggttttc gagttcgtcg aattcactat gtggtcgcca
      421 caaatacgac acgcccgatt tcccttgaga gggtagcacc tggtgcactc ggatattttc
      481 ggactccgag aggcgtctaa aaaaggcctg ctccacttcg gtccattcgc aaaatcccca
      541 gagatatacc tcgattggtg aattgtgttt tgcaggctcg ccgaatgctt ctaaaactcg
      601 ttccgaagcc tggcgtagaa gttccgcccc gctccaaggc caaaacactc cccgtttcca
      661 gagcaagacg agggcctcta ataatttttt ttgagattcg aaacgttgcc cctctcgttc
      721 cgacactgcc tcgagggtgg cctcgagttg ctgtagatcg gaccaagcat agtcaaaacg
      781 cgtgacataa gctgccaaac gtttggcaat cgaacgcgtt cgcaatgctc gggccaactt
      841 cgaatcacct gctcgaaagt tcggatcttt agaaatcact tctagtgcgt caatctgagc
      901 cgcacgactg agcggcacag ccaatggggc gccagcccgc attaaaattt cacgggcaaa
      961 ttcttcaaca ccggtaaccg atccactcgc gacaccgggc acccgttcga gcaaccattc
     1021 cttaattacg gttcgcgaac catggggacc aaccaaaacc cgaacgcgat caccccacgg
     1081 ctgatcacca cgcacctgtt cccatacgtc ttcgagttgg ttgggagtga tgacttcaaa
     1141 gaaagaacga tcggtatgca ttagtagttc aactgcaaaa gtttctcttg cactcggatt
     1201 cgcacggaac tttgttggcg ttttttccgc cgcaccactt gtccggcgac cacgccagga
     1261 ctaccgctcc ggcgtagtgc cccaagcgct ttactcaccg cacgcggcgc gactgcgatc
     1321 acaag
//
LOCUS       DAOMXO010000690         1320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_60160_length_1320_cov_2.920949, whole genome shotgun sequence.
ACCESSION   DAOMXO010000690 DAOMXO010000000
VERSION     DAOMXO010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60160_length_1320_cov_2.920949"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..>1320
                     /locus_tag="PLH57_11590"
     CDS             40..>1320
                     /locus_tag="PLH57_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03298.1"
                     /translation="MIEANQIVRQSKFGIEELLRANARVLYEYFFSTRHVGRVLDFLF
                     YEAKDALAAVDDGEVLEYRVRQILETSIFNGFANYRTGEKSKCIEIEYGWDDVRMITS
                     AVFWVEQTCAGFMPGEKPNDGVAGRIQKFIENISTLADEVVVRFDPRSGRFQIVGIIN
                     TAGNDVATKKAIDYVDVSALSPSEDGNSTHSTQTAASTVQPEALSAADIKDFTEDEKK
                     FERERGRLTHVKEAEEEKQAVIEARVHELEERKRKKAMLDRRSTGSTVEDPEIISRER
                     RSHENLADLEFRDREKPSKGTAFSFVEHLEKQANDQIETKKRTALGERDRQLIADTSS
                     DVIRVRGGGSESEQDGPEVVLKTERLSTDEEAVSVGAIDRINTRDDFLTVKNLSIDLS
                     KAPVESPLSEKENEDVEEVLQLLLKAVRAELVVVL"
BASE COUNT          394 a          290 c          342 g          294 t
ORIGIN      
        1 gaatttttcg ctgaccgacg ccccaagaaa ggcggcggca tgatcgaagc gaatcagatc
       61 gtacgccaat ccaaatttgg aatcgaagaa cttcttcgag caaatgcgcg agtcttgtac
      121 gagtactttt tttcgactcg tcacgttggt cgagtgttgg attttctatt ttatgaagcc
      181 aaagacgctc tggccgcagt ggacgatggg gaggtgcttg agtatcgagt gcgtcagatt
      241 ctcgaaacct caattttcaa cggatttgcc aattaccgaa ccggcgaaaa gtctaagtgt
      301 atcgaaatag agtacggttg ggacgacgta cgtatgatta ccagtgcggt attttgggtc
      361 gagcaaacat gcgctggttt tatgcctggc gaaaaaccga acgacggagt cgcgggtcgc
      421 attcaaaaat ttattgaaaa tatctccacg ttggccgatg aggtcgtcgt ccgatttgat
      481 ccccggagcg gccggtttca gattgtcgga attatcaata ccgctggaaa cgacgtcgcc
      541 acaaaaaagg caatcgacta cgtagacgtt tcagcacttt ctccctcaga ggacggaaat
      601 tctactcact caactcagac cgctgcttca acagtacaac cggaagctct ttcggccgct
      661 gatataaaag attttactga agacgagaaa aaatttgaac gcgaacgcgg gcgcctaact
      721 cacgtaaaag aagccgaaga agaaaaacaa gcggtgattg aagctcgtgt ccatgaacta
      781 gaagagcgca aacgaaaaaa agcaatgctc gatcgaaggt ccactggctc gactgtagaa
      841 gatcccgaaa ttatttctcg cgaacgccga tcgcacgaga acttagcaga tttggaattt
      901 agagaccgcg aaaagccgag caagggaact gcgtttagtt ttgtagaaca cttagaaaag
      961 caggccaatg atcaaatcga aactaaaaag agaaccgctc taggggaacg agatcggcaa
     1021 ttgattgcgg acaccagtag cgacgtaatt cgggtgcggg gtggcgggtc cgagtcagaa
     1081 caagacggac cggaagttgt cttaaaaaca gaacgcctgt cgaccgacga ggaagcagtg
     1141 tcggtcggag cgattgaccg aattaacact cgagatgatt ttttaacggt gaaaaatttg
     1201 tcaatcgatc tctccaaggc tccggtcgag agcccactat ctgaaaaaga aaacgaagac
     1261 gtagaagagg ttttacaact tttactcaag gccgtacgtg ccgagcttgt agtagtactt
//
LOCUS       DAOMXO010000691         1319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_60235_length_1319_cov_2.496835, whole genome shotgun sequence.
ACCESSION   DAOMXO010000691 DAOMXO010000000
VERSION     DAOMXO010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60235_length_1319_cov_2.496835"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PLH57_11595"
     CDS             complement(<1..1027)
                     /locus_tag="PLH57_11595"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRK03299.1"
                     /translation="MTRDRGKKRFRKSILDNGMVLVTESVPSFHSLAIGVWVRAGTRH
                     ESAHEAGLSHFLEHMMFKGTEKRSALDIAQQIDRVGGEFNAFTAREYTCFHVLLLARD
                     YTLAVDILSDILHNSTFDEIEIERERKVILQEISMVEDNPEELAHDLFFEQAFGGHAL
                     GKPILGTRDTLKKFNRSTVYSYFRKHYAPGEIILSVAGDVDHFKVRDSLNKTLKPEYL
                     RVKERRKHNMQRPRVRRGTQVIRRNLEQAHVLIGFPSVAYNHGDRFVAYLMSNYLGGG
                     MSSTLFQEIREKRGLAYTIYSTNSAFSDSGVFSTYVATNSEGVKTCLDITAKQIEKLK
                     NEKLDSKA"
     gene            complement(1052..>1319)
                     /locus_tag="PLH57_11600"
     CDS             complement(1052..>1319)
                     /locus_tag="PLH57_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRK03300.1"
                     /translation="HVSEIAHQRVNNVRDILKEGEKLDVKVLEVDRNGKIRLSRKALL
                     ERPEGMPEPTGGGGGPRGPRGDGGGGARGPQGGGRGGQRPMDPR"
BASE COUNT          322 a          342 c          302 g          353 t
ORIGIN      
        1 gtgcttttga atcaagtttt tcatttttta gtttttcgat ctgcttggca gttatgtcga
       61 gacaggtttt cactccttcg gaatttgtcg caacataggt cgaaaaaacg ccagaatctg
      121 aaaaagccga attggttgaa tagatggtat aggcaaggcc tcgtttttcg cgaatttcct
      181 gaaagagcgt agaactcatg ccaccgccca ggtaattgct cattaaatag gctacgaagc
      241 gatcaccgtg attgtaggca acactgggga atccgatcaa cacgtgggcc tgttctagat
      301 ttcgccgaat tacttgagtt cctcgccgca ctcgcggacg ctgcatattg tgttttcttc
      361 gttcttttac tcgcaagtac tccggcttaa gggttttatt aagggaatcg cgaactttaa
      421 aatggtcaac atcaccggca accgaaagaa taatttcacc tggcgcgtag tgttttcgaa
      481 aatacgaata aaccgtagag cgattgaatt ttttcagagt gtctcgcgta cccaagatcg
      541 gtttgccaag cgcatgccct ccgaatgctt gctcaaaaaa taaatcgtgt gcaagctctt
      601 cggggttatc ctcgaccatc gaaatttctt gaagaatgac tttgcgctca cgctcaattt
      661 caatttcgtc gaaagttgag ttatgcaaaa tgtccgatag aatatcaacg gctagcgtat
      721 aatcgcgggc gagtagcagt acgtggaaac aagtgtattc acgagccgta aacgcattga
      781 attccccgcc tactcggtcg atttgctggg caatgtcgag tgcgctgcgc ttttccgtcc
      841 ccttaaacat catgtgttca aggaaatgag acaaccctgc ttcatgggcc gactcatgcc
      901 gcgttccagc ccgaacccag acgccaatcg ctagtgaatg aaacgacggc acgctctcag
      961 ttacgagaac catgccgttg tccaaaatcg attttctaaa ccgctttttc ccgcggtctc
     1021 gagtcatcgt gatgctgatt ctttctcggt cttagcgagg gtccattggg cgctgaccac
     1081 cacggccacc accttgagga cctcgagcac cgccaccacc gtcaccgcga gggccgcggg
     1141 gacctccccc accgccggtc ggctctggca ttccctcagg tctttcaagc aacgctttac
     1201 gactcaaacg aatcttgccg ttgcgatcaa cctcgagaac cttgacgtca agtttctcac
     1261 cttctttaag aatgtcacgc acattgttta cacgctggtg agcaatttct gaaacgtgt
//
LOCUS       DAOMXO010000692         1318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_60252_length_1318_cov_7.255740, whole genome shotgun sequence.
ACCESSION   DAOMXO010000692 DAOMXO010000000
VERSION     DAOMXO010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60252_length_1318_cov_7.255740"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..>1318
                     /locus_tag="PLH57_11605"
     CDS             81..>1318
                     /locus_tag="PLH57_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03301.1"
                     /translation="MRAHINNFGYTITAFDQLRTQPFGAAASFVADRYFTPLMGASRP
                     MRKLMDSTIIYSKEVNGGAVVNGKLIGRALLVGGIDVAGYAAQLIWLYPRIASEVAQA
                     FDSIAPNISEAMRDTFVNQNLETAKIRNVDIHSTTQDNILKMSAHYTVLNDFNTQQHT
                     GAVNAKMIHDGLNPEDPRNAAEKKRRLNEMVDIANQRGGLAERSEFVWDLTVVLAEIK
                     NFLGYKLSDEERAAVGQQTVMGEHHPGFIFPALKAARKHAKSELKRDPNNNQAREALN
                     LFEETIADFGILHSLVKSVNLREDFIREASRFRRVRRTLLLLTTEGESIEPLLEVISE
                     WSKRISIGSAAWAANVYTTKFIEIISGGKLRMMPTPEESEKFGLEAEKQALAEMQVLF
                     EREFLSGVDLQKAHPVTFQV"
BASE COUNT          367 a          300 c          339 g          312 t
ORIGIN      
        1 ttcgtgagtt gtggcaccaa ttccttacct ggaaacaacg cccaaaacac cgcggcgtcc
       61 gctaccatct gcgccgcgag atgagggccc atataaataa tttcggttac accattacgg
      121 catttgatca attgcgtacg cagccgtttg gcgcagcggc gagtttcgtg gcagatcggt
      181 actttactcc actcatgggc gccagcagac caatgcgcaa actgatggat tccaccatta
      241 tttactccaa agaagtaaac ggcggcgcag tggtcaacgg aaagctcatt ggtcgcgcgc
      301 tcttagtcgg tggcattgac gtcgcaggtt acgctgcgca gttaatttgg ttgtatccgc
      361 ggattgcgag tgaagttgca caggcattcg atagcatcgc gcccaatatt tctgaggcaa
      421 tgcgtgatac cttcgtgaat cagaacttag aaactgccaa aattagaaac gtcgacattc
      481 atagcaccac tcaggacaac attttaaaaa tgagtgcgca ttacactgtg ttaaacgatt
      541 tcaatactca gcagcacacg ggcgctgtta atgcgaaaat gattcacgac ggattaaatc
      601 ccgaagaccc gagaaacgca gcagaaaaaa aacgacgact aaatgaaatg gtcgacatcg
      661 ccaatcaacg cggcggactt gccgaacgta gtgagtttgt ttgggatctc actgtcgtct
      721 tggcggagat caaaaatttt ctaggttaca aattaagcga cgaagagcgt gccgcagttg
      781 gccaacagac ggtgatgggt gaacaccacc ccgggtttat ttttcctgcg ctaaaagctg
      841 cgcgtaagca cgcgaaaagt gaactcaagc gcgatccaaa taacaaccag gcacgcgagg
      901 cgctgaatct tttcgaagaa accattgccg actttggcat tcttcacagc ttggtaaagt
      961 ccgtaaatct aagagaagat ttcattcgtg aggcttctag gtttcgacga gtaagacgaa
     1021 ctctgttgtt gctgacgact gagggcgaga gcattgaacc gttactcgag gttatttcgg
     1081 agtggagtaa acgaattagt attggcagtg cagcttgggc ggctaacgtt tacaccacca
     1141 aatttatcga aatcattagc ggtggcaaat tgcgaatgat gccaaccccc gaggaaagtg
     1201 aaaagtttgg actagaggcc gagaaacaag ctctggcgga aatgcaggtt ttgttcgagc
     1261 gggaattttt gagtggtgtg gacctgcaaa aggctcatcc ggttacgttt caagtttt
//
LOCUS       DAOMXO010000693         1315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_60469_length_1315_cov_3.894444, whole genome shotgun sequence.
ACCESSION   DAOMXO010000693 DAOMXO010000000
VERSION     DAOMXO010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60469_length_1315_cov_3.894444"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1203)
                     /locus_tag="PLH57_11610"
     CDS             complement(16..1203)
                     /locus_tag="PLH57_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03302.1"
                     /translation="MRRLKTDVAIIGSGTLAIFIAQALLAKSLRVVLINPLKEFDCSD
                     IRPHAGLGLWNAIYRSQQSQQSQFGSTLSGYEHELERRLRDLFPAPVQNSGLRRLDQW
                     TLLSGTPIHKAHTDDFEKEYFRLEKKNWASGHVRLINPDHALTILRKQGLEVQRVAQL
                     EGAFVRNYALWWDAPKILSFLLHFLKNRFSQAGAERLLSHKHDAFLFKGVKIRGRYGR
                     RVVIEGSDFEEVSVEPESNLMICLTGDLLPTMRSVVAACDEPWIQGVRKRRREQHFAW
                     FNSPTLTCDSNAFWIEIGGTKYLCGKGGADLRESGSAAYATWTSERGPDALDSVVDEG
                     FRILDSGSRKPRFVRSERTFRLEWDWKNPQWRTTAHDTQWATAFEGDLWSTLELAWNV
                     PLS"
BASE COUNT          347 a          342 c          289 g          337 t
ORIGIN      
        1 gagtacgaaa atttgttaag acagaggaac gttccatgcc agctccaatg tggaccagag
       61 atcaccttca aatgcggtgg cccattgagt atcgtgtgcc gtagtgcgcc actgagggtt
      121 tttccaatcc cattctaggc gaaatgtacg ttcacttcgt acaaatctcg gtttgcgaga
      181 tccggaatct aatattcgaa acccttcatc aacaaccgaa tcaagcgcgt cggggcctcg
      241 ctccgaagtc caggtcgcgt aagcggccga cccggattcg cgcaaatccg ctccaccttt
      301 tccacaaaga tatttcgtac ctccgatttc gatccaaaaa gcattagaat cgcaggttaa
      361 ggtgggactg ttaaaccaag caaaatgttg ctctcgtcgc ctctttcgaa ctccctgaat
      421 ccagggctcg tcacaggccg ccaccacact tcgcatagtg ggtaacaaat cgcccgtaag
      481 acaaatcatt aaatttgatt cgggctctac gctaacctct tcgaagtcgc tgccttcgat
      541 aacaacgcgt cgaccatagc gacctcgaat tttcacacct ttaaataaaa aagcgtcatg
      601 tttatggctc aatagccgct ctgcaccagc ctgggaaaat cgatttttca aaaaatgtaa
      661 aagaaatgaa agaatttttg gggcatccca ccacaaggcg tagtttcgca cgaaagcacc
      721 ttcgagctgc gccactcttt gcacttcgag gccctgcttt cgcaatatcg tcaaggcatg
      781 gtctgggtta attaatcgaa catgaccgga ggcccagttt tttttttcta atcgaaaata
      841 ttctttttca aagtcgtcgg tgtgggcctt atgaataggc gtaccactta aaagcgtcca
      901 ctgatccaac ctacgcaaac ctgagttttg aactggcgct ggaaacaaat ctcggagtct
      961 acgttcaagt tcatgttcgt aaccggaaag tgtagaccca aactgcgact gttgactttg
     1021 ttgcgagcga taaattgcgt tccacaggcc caagcccgca tgagggcgaa tatcgctaca
     1081 gtcaaattct ttcaatggat tgattaaaac tactcgaagt gactttgcca gcaatgcttg
     1141 ggcaataaat atcgcaagcg tacccgaacc aattatcgcc acgtcggttt ttagcctacg
     1201 catgtggggt ggattcccgt tcggcaagct cccccatcac tcggcgcatt gctgcttgaa
     1261 tctgcgcctt tttataagct gaatgggcgc tcattccagc aattgaatca cggtg
//
LOCUS       DAOMXO010000694         1315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_60477_length_1315_cov_3.536508, whole genome shotgun sequence.
ACCESSION   DAOMXO010000694 DAOMXO010000000
VERSION     DAOMXO010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60477_length_1315_cov_3.536508"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLH57_11615"
     CDS             <1..876
                     /locus_tag="PLH57_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRK03303.1"
                     /translation="AVSGLPQVILSLYGNEVQKKKYIPRLASGEAVGAFSLSESSAGS
                     DAAGLKTKATKHGDEYVLNGTKLWTTQGNVAETVVVFARTGGDGPKGVSAFIVEKDTP
                     GFRAGKKEAKMGMNISPTCEMILEDVKVPAQNLIGREGQGLKIALSALDSGRITIAAT
                     AIGVARAALEYSTQYSKGRTQFGKAIAEFQGIQFMLADMATALEASRLLVFRAAELRD
                     QKLPFTMQAAMAKMFATDSAMKITTDAVQILGGNGYVREYPVERYMREAKAAQIVEGT
                     NQIQRMIIGRALTKD"
     gene            899..>1315
                     /locus_tag="PLH57_11620"
     CDS             899..>1315
                     /locus_tag="PLH57_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03304.1"
                     /translation="MSHLQVDLHALGTPVSRDSVSSALEHFISDMKAGKSGFYALPKQ
                     NSFPSEILERKAVLASRMGRVETFIHLGIGGSSLGAEAIVRALAPTNAPAFYFLDNID
                     PDALCDVMTVADPATTLIYVVSKSGNTLETIAQFEVL"
BASE COUNT          360 a          320 c          323 g          312 t
ORIGIN      
        1 gcagtaagtg gtctgcctca agtaattttg agtctctacg gcaacgaagt tcaaaaaaag
       61 aaatacattc cgcgactcgc aagcggtgag gcggtgggtg ccttcagtct ttctgaaagt
      121 agcgcgggct ccgacgctgc gggcctcaaa acaaaagcca caaaacacgg agacgaatat
      181 gtcttaaacg gcactaaact gtggaccacc caaggcaacg tcgcagaaac cgtggtggta
      241 ttcgcacgta ccggcggcga tggtccaaaa ggtgtttctg cttttattgt tgaaaaagat
      301 accccgggct ttcgcgccgg aaaaaaagaa gccaaaatgg gaatgaatat ttcgcctacc
      361 tgcgaaatga ttctcgaaga cgtaaaagtt ccagcacaaa atcttattgg tcgagaagga
      421 caagggctta aaatcgcact aagcgctctc gatagtgggc gtattacgat cgccgcaacc
      481 gccattgggg tagcccgcgc ggctttagaa tattcgaccc agtactcaaa gggccgcacg
      541 caattcggaa aagctattgc cgaatttcaa ggcattcaat tcatgctagc agacatggcc
      601 actgccctcg aagcttcgag acttctagtt tttagagctg cggaacttcg agatcaaaag
      661 ctgccgttta ccatgcaggc tgctatggca aaaatgtttg ccactgattc tgctatgaaa
      721 attacgaccg atgcagtgca aattttgggt ggcaatggtt acgtacggga ataccccgtc
      781 gaacgataca tgcgcgaggc gaaggccgcc cagatcgtcg agggtaccaa tcaaattcag
      841 cgcatgatca ttggacgcgc tctcaccaaa gattaatcac aaggaataac accaccacat
      901 gagccactta caagtcgatt tgcatgccct tggtactccg gtttctcggg actccgttag
      961 ttccgcactc gaacacttta tcagcgatat gaaggctgga aagagcggtt tttatgctct
     1021 tccaaaacaa aattcctttc ccagcgaaat actggaacgc aaagcggtgc ttgcaagtcg
     1081 aatgggcaga gttgaaacat ttattcactt gggaattggc ggctcgagtc tgggcgccga
     1141 agccattgtt cgcgctctgg caccgactaa tgctcccgca ttttattttt tagataatat
     1201 tgaccctgac gccctttgcg acgtcatgac cgttgcggac ccagctacta ctttgatcta
     1261 tgtagtgagt aaatcgggta atacgctcga aactattgcg cagtttgaag tgctt
//
LOCUS       DAOMXO010000695         1308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_60930_length_1308_cov_4.035914, whole genome shotgun sequence.
ACCESSION   DAOMXO010000695 DAOMXO010000000
VERSION     DAOMXO010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60930_length_1308_cov_4.035914"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..>1308)
                     /gene="purB"
                     /locus_tag="PLH57_11625"
     CDS             complement(51..>1308)
                     /gene="purB"
                     /locus_tag="PLH57_11625"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRK03305.1"
                     /translation="WLFVELANLHALEKRGAVPEGVAARTEKKAKIKVSRIQELDLEL
                     KHDVIAFTTSISEQIGDDGRFFHYGLTSSDVVDTALSMALVQATDLIVDRIKTFEDTL
                     ETWALRLKNQPSIGRTHGVHAEPTSFGLKFLGWLEEMRRSRVRLKAARENIRYGKLSG
                     AVGSYGAISPEIEIDALSLLKLKVEPIATQVVPRDRHAELFCGLAILGASIERVAVEL
                     RHLQRTEVHEIEEAFSTKQKGSSAMPHKKNPISAENLTGCARLLRSYALAALDNVALW
                     HERDISHSSVERVICPDAFILADYAMDRLNRLVSGLKILKSNIEKNLDKTQGIYFSGH
                     VLLKLVEKGMSREQAYRIVQKRAHVAWDKGTWYGDELWKESAIRARFSRKELNRIMDV
                     RNYLRHVPKIYKRVLEPSEKKRSNNR"
BASE COUNT          323 a          340 c          271 g          374 t
ORIGIN      
        1 aatatgtaat gttcatcgcg agcgccaaca tcacgatgaa cattacatat tcaacggttg
       61 tttgacctct ttttttccga tggttccaac actcgtttgt agatctttgg tacatgacgc
      121 aaatagttcc tcacgtccat tatacgatta agttccttcc gtgaaaaccg tgcgcgaatt
      181 gccgactctt tccacagttc gtcaccgtac caggtgcctt tatcccaggc tacgtgcgcc
      241 cttttttgga ctatgcggta ggcctgttcg cgactcatgc ctttttcaac gagtttcaaa
      301 agaacgtgcc ctgaaaagta aattccctga gtcttatcta agttcttttc aatattcgac
      361 ttcagaattt taagtccaga aaccaagcga ttcaatcgat ccatggcgta atcggcaaga
      421 ataaatgcgt cgggacaaat cacacgctct actgaagagt ggctgatatc acgttcatgc
      481 cacagagcta cgttatcgag ggcagccaat gcatagctgc gcaatagacg tgcgcaaccc
      541 gtaaggttct cggcagaaat gggatttttc ttatggggca tcgccgaact gcctttttgc
      601 ttcgttgaaa aagcttcctc gatctcgtgc acttctgttc tttggagatg ccgtagttcc
      661 actgcaactc gctcaatcga ggcgcctaat atcgccagcc cacaaaacaa ttccgcgtga
      721 cgatcccggg gaacgacttg cgttgcgatt ggttctactt ttaatttaag taacgacaga
      781 gcatcaatct caatctctgg gctaatcgca ccgtaactgc cgactgcgcc cgacaacttt
      841 ccgtaacgta tattttcgcg ggctgctttc aaacgcactc tggaccttcg catttcttcc
      901 aaccaaccaa gaaatttcaa accaaagctc gtcggctcgg catggacgcc atgagttcgt
      961 cctatcgaag gctgattttt tagtctcagc gcccacgttt cgagagtgtc ttcgaatgtc
     1021 ttgattcgat ctacaattaa atctgtggct tgaacaagag ccatcgacaa agccgtgtcg
     1081 acgacatctg aactagtcaa tccgtagtga aaaaaacgcc cgtcatcgcc tatttgctcg
     1141 ctaatgctcg tcgtaaacgc gattacatcg tgttttaatt ccagatcgag ttcttgaatt
     1201 cgcgaaactt taatcttcgc ttttttttcg gtccgagcgg ctactccttc gggaacagct
     1261 cccctttttt cgagagcgtg aagatttgct aattccacga atagccaa
//
LOCUS       DAOMXO010000696         1305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_61127_length_1305_cov_5.352800, whole genome shotgun sequence.
ACCESSION   DAOMXO010000696 DAOMXO010000000
VERSION     DAOMXO010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61127_length_1305_cov_5.352800"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..>1305)
                     /locus_tag="PLH57_11630"
     CDS             complement(107..>1305)
                     /locus_tag="PLH57_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03306.1"
                     /translation="SCWGQNTYQQAGTAASEPVVRAPNPIYYWSSQGQIFPTKFYEPS
                     SLAAGYGHACGVQNGRVVCWGYNSDGQLGNGLTTLSDRYGRFVKINSTTDLTEATSVA
                     LGAYHSCAIVGAARRLYCWGKNTSYQIGNGSDPKVYYARAVTTGPTNAVIDNVAQVFA
                     GTTHTCVVYAVTGGVSCFGSNVFGERGNNSTSTTASSTPAAVVIADSHNSFPPFTAVT
                     EARSRNYHSCGVNSGGALFCWGYNAFSNLGSLIPATTARVASAQRVYDGPGPLADVRS
                     LGAGAYHSCAIVGVNRTVKCWGRNDYLALGVSEVVVPTHSNVPRVVEGLNGAPALENV
                     SQISLGEDHSCALLSNGRVVCWGRSNSAQTGYQEDDFETVNEHSTPVPIYRINRHPPP
                     PEESPA"
BASE COUNT          359 a          361 c          307 g          278 t
ORIGIN      
        1 ggaaatcaag gcccccatcc acaacaatga aacgatcgaa tacaccagtc ccgctaagcc
       61 aacaaaagaa actattatta aaacagcttc gcgacgaatc atgctcttag gcaggacttt
      121 cttcgggagg cggcggatgt cgattaattc tataaatagg aaccggagtc gaatgttcgt
      181 tcaccgtttc aaagtcatct tcctgataac ctgtttgagc gctgttggat cgcccccaac
      241 acaccactcg cccattcgaa agaagcgcac agctgtggtc ctcacccagt gaaatttgac
      301 tgacattttc aagcgccggt gcaccgttga gtccttcaac cactctcggc acgttggaat
      361 gagtaggaac aacaacttca gaaacaccga gtgcaaggta atcgtttcgg ccccaacatt
      421 ttacagttcg attaacgcca acgattgcgc aactatgata tgcgcccgct ccaagacttc
      481 gaacatccgc caaagggccg ggcccgtcat agacacgttg agcactcgcc acacgcgcag
      541 tggtcgccgg tattagcgat cctaaattgg aaaaggcatt gtaaccccag caaaaaagcg
      601 cgccgcccga attcactcca caggaatgat agttgcgaga tcttgcctca gtcactgctg
      661 taaacggcgg aaaactattg tgcgaatccg cgataacaac tgctgcagga gtagacgatg
      721 cagtcgttga ggtggaattg ttgccacgct caccgaaaac attcgatcca aaacaggaca
      781 cgccgcccgt gacggcataa acgacacaag tgtgagtcgt tcccgcaaac acttgcgcaa
      841 cgttgtcgat cactgcgttc gtcggtcccg tggtaacagc gcgtgcgtaa tacaccttgg
      901 ggtcagagcc gttaccgatt tggtaagaag tatttttacc ccaacaataa agacgtctag
      961 cagccccgac gatcgcgcag gaatgataag cccccagcgc cacggaagta gcttctgtta
     1021 agtcagtcgt agaattaatt tttacgaatc gcccgtagcg atcactcagc gtggttagac
     1081 catttccaag ttgtccgtca ctattgtagc cccaacaaac gacccgacca ttttgcactc
     1141 cacacgcgtg gccataaccc gcggctaacg aagagggctc ataaaacttg gttggaaaaa
     1201 tctggccctg agatgaccaa taatagattg gattaggcgc ccgaactacg ggctcggatg
     1261 ccgcggttcc agcctgctgg taggtgttct gcccccaaca agaaa
//
LOCUS       DAOMXO010000697         1305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_61136_length_1305_cov_4.011200, whole genome shotgun sequence.
ACCESSION   DAOMXO010000697 DAOMXO010000000
VERSION     DAOMXO010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61136_length_1305_cov_4.011200"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLH57_11635"
     CDS             complement(<1..232)
                     /locus_tag="PLH57_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRK03307.1"
                     /translation="MGKNPLQGGFGNFMKQAQQMQQRIQRAQSEVAEKTAEGSAGGGV
                     VKVVANGKQEILSVTINKEAVNPDDVEMLQDMV"
     gene            complement(243..>1305)
                     /locus_tag="PLH57_11640"
     CDS             complement(243..>1305)
                     /locus_tag="PLH57_11640"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03308.1"
                     /translation="DALVILAREAEGSMRDGVSLLDQVLASNDVGGKISAQAVTNSLG
                     LVDKQTVLDCVSAILKREPLKALEAAGLVHLHGFDLKQFGREILRTLRTIMVVKLIEE
                     KNADAAPFLDLSDVDLADLRALAPLREIGDLDLLFLILNHGLEDVARSAIPKTVLDVL
                     LIKMASSEELVKIDSDSVSESIPTAPRAPTPAPTQQTKPAATAPSASATPSTKPSIDW
                     ASAGVDFWKQAVAYVKSKKPLIGNVIENASFERLKKHEDHLLVTLGFNKDVSFYKDQL
                     QSRAYMEQVQTLLKAFAGVPTRLELVESATNRSLNAIEEEKTKNSIDQKRKTILESDA
                     LKATEQLLGAKLERLDLQN"
BASE COUNT          309 a          311 c          318 g          367 t
ORIGIN      
        1 gtaccatatc ctgtaacatt tctacgtcgt cggggttcac agcttcttta ttgatagtca
       61 ctgatagaat ttcttgtttg ccgttggcta ctactttcac cacaccacca cctgcgctac
      121 cttcggcggt tttttccgcc acctcggatt gtgcgcgctg aatgcgctgt tgcatttgtt
      181 gtgcttgttt catgaaattg ccaaagccac cctgcagagg atttttcccc atgttgttct
      241 ccttagtttt gaaggtcgag gcgttcgagc ttggcaccga gcaattgttc ggtggccttg
      301 agcgcgtcgc tctctaaaat agtttttctt ttttgatcga tagagttttt agttttttct
      361 tcttcaattg catttaaaga ccgattggtc gcgctctcta ctaattcgag ccgagtaggc
      421 acccctgcaa acgccttgag cagggtttgt acttgttcca tgtaagcgcg agattgaagc
      481 tggtctttgt aaaatgaaac gtctttattg aaacctaacg taacgagcag gtggtcttcg
      541 tgtttcttta gacgctcaaa actcgcgttc tcgatcacgt taccgatgag aggttttttg
      601 ctctttacgt aagcgacggc ttgtttccaa aaatcaacgc cagcagaggc ccaatcaatg
      661 gagggcttgg tgctaggagt agcactcgcc gagggagcag tagcggcggg cttggtctgt
      721 tgagttggtg ccggggtcgg ggcacgcggg gctgtaggaa tcgactcaga aacagaatcg
      781 gaatcaattt tcacaagctc ttcggatgag gccatcttga tgagcaacac atcgagtacc
      841 gttttaggaa tggcgcttcg ggctacatct tctaatccgt gattgagaat caaaaatagc
      901 aaatctaggt cgccgatttc acgcaaagga gcaagcgctc gcaaatcggc gagatcgacg
      961 tccgacaaat caagaaaagg tgcggcgtca gcattttttt cttcgatcaa tttaacgacc
     1021 atgatggtgc gcaaagtgcg tagtatttcg cgaccaaact gttttaaatc aaacccgtga
     1081 aggtgtacaa gacctgcggc ttcgagtgct ttaagtggtt cgcgcttgag aatggcggaa
     1141 acgcaatcaa gcacggtttg tttgtctacc aaacccaacg aattcgtaac cgcttgagca
     1201 gaaattttgc caccaacgtc gttcgacgcc aaaacttgat caagtagaga caccccgtcg
     1261 cgcatactac cttcagcttc acgcgctaga ataaccagtg catcc
//
LOCUS       DAOMXO010000698         1296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_61749_length_1296_cov_4.170024, whole genome shotgun sequence.
ACCESSION   DAOMXO010000698 DAOMXO010000000
VERSION     DAOMXO010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61749_length_1296_cov_4.170024"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PLH57_11645"
     CDS             <1..1272
                     /locus_tag="PLH57_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03309.1"
                     /translation="RGVDCASFANAKMGYEALKHEGKAVSLVILSKEQTEENDGLAIL
                     EAIRKEYHIRNTPVILLSSIWGKAEFAKHQKSESGANGYFNKSTPVTEFESVIESVLG
                     GKFIARKPVAPAAPQKDAGAPPPSPIPEPGSISSITLEVSNSGAEPESSDSPVQLFEA
                     KEVLGGAPVDKISIDFGPAVEGGASSVAQGAAVPPSPPEATVVPDLSSVSVTMAAPQL
                     EKLVAQSEAAKKSVEPEPITIAPTSEPVALQQLTGVTNMASDIKLETTQLPTPPSTPE
                     LVEAPDTKLSDEDVAKELPYLFSENTTTAPPSNLTRLTSMGAKRPAYDVKISLPGSEF
                     SAGGVGPAASGLEAAPDDVETLKRYLAMREQDVSVLTVQLTYAKEELAKSQNQIQEMA
                     LQNEDLFHQLDDLKRKYTELQAEQVHKGASV"
BASE COUNT          363 a          319 c          338 g          276 t
ORIGIN      
        1 cgtggtgtgg attgcgccag ctttgctaac gcgaagatgg gttatgaagc cctgaaacat
       61 gaaggcaagg cagtttcttt ggtgattctc tcgaaggagc aaaccgaaga gaacgacgga
      121 ctcgcgattt tagaggcgat ccgaaaagaa taccatattc gaaacacacc agtgattctg
      181 ctctcttcga tctggggaaa ggcagagttt gcgaagcacc aaaaaagcga aagcggcgca
      241 aacgggtact tcaataagtc cacccctgtc acagaatttg agtctgttat cgagtcggtt
      301 ttaggcggta agttcatagc tcgaaagcca gtggcaccgg cggctcccca gaaagatgcg
      361 ggcgcgccac ctccttcacc gatacccgag ccaggatcaa tttcgagtat tacgttagag
      421 gtatcaaata gtggtgcaga acctgagagt tcagactcgc ccgttcagtt attcgaggcc
      481 aaggaggttc ttggcggagc tccagtagat aaaatttcga tcgattttgg gccggcagtg
      541 gaaggtgggg cttccagtgt tgctcaaggg gcagccgttc ccccgagtcc gcccgaggca
      601 acggtggtac cagatttgtc gtcggttagc gttacgatgg ccgcaccaca actcgaaaaa
      661 ctagtggccc aaagtgaagc agccaaaaaa tcagttgagc cggaacctat tactatagcg
      721 ccaacctcgg aacccgttgc tctgcaacaa ctaacgggtg tcacgaacat ggcgtccgat
      781 attaagctgg aaacaacgca gcttccaaca cctccgtcga cgccagaact agtcgaggca
      841 cccgacacaa agttaagtga tgaagacgtt gctaaagaac ttccgtattt attttctgaa
      901 aataccacta ccgcaccgcc ttcgaatttg acgcgtctaa cgagcatggg ggcaaaacgt
      961 cctgcttatg acgtaaaaat cagtctcccg ggttcagagt tttctgcggg aggggtgggg
     1021 cctgcggcga gcggattaga agccgcgccc gatgacgtgg aaaccctaaa acgctacctc
     1081 gccatgcgag agcaagacgt ttcagttctc accgttcaat taacttacgc caaagaagag
     1141 ttagcaaagt ctcaaaatca aattcaagaa atggcgcttc agaatgaaga cctatttcac
     1201 cagttagacg acctaaaaag aaagtatact gagcttcaag ccgaacaagt tcacaaagga
     1261 gcgtctgtat gaggcgaact cgatgcagcg cttttg
//
LOCUS       DAOMXO010000699         1284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_62633_length_1284_cov_3.160293, whole genome shotgun sequence.
ACCESSION   DAOMXO010000699 DAOMXO010000000
VERSION     DAOMXO010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62633_length_1284_cov_3.160293"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLH57_11650"
     CDS             <1..396
                     /locus_tag="PLH57_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03310.1"
                     /translation="ALLLGVGISPLPAAECPSDTLAKSIRIAAKQDGENSERGPTLYL
                     VCGRKSGIAKTEKLPYACPPKTNPEYIRRGNYYWVECYKTQREVSYVFQFQLTGNGAK
                     LISSGRPTPEPKTLRPASDASDDAVKTVD"
     gene            complement(419..>1284)
                     /locus_tag="PLH57_11655"
     CDS             complement(419..>1284)
                     /locus_tag="PLH57_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRK03311.1"
                     /translation="ATAPVLVAGSDEQKKKYLGKLVSDFTFAAYCVTEPNAGSDVAAI
                     RTTAKKVGNEYVINGSKMWITNGSVADWYFVLAVTDPAAKHKGLTGFIVDAKSPGITV
                     GKKEWNMGQRASDTRGITFEDVKVPATNLLGKEGDGFKIAMGAFDHTRPSVAAGAVGL
                     ARRAMDEAINYSQTRKTFGQPIAAYQGVSFMIADMAKDIEAARMLVWRAAWEIDQGRR
                     NTKFAAFAKAFAADMAMRVATDAVQVHGGYGYSSEYPVEKLMRDAKIYQIYEGTSQIQ
                     RLIIAKEIFER"
BASE COUNT          304 a          326 c          333 g          321 t
ORIGIN      
        1 gcgctccttt taggagtggg gatttcacct ttgccggcag cggaatgtcc gagcgataca
       61 ttagcgaaaa gtataagaat cgccgcgaaa caagatggcg aaaactccga gcgagggccg
      121 actctttacc tggtgtgtgg tcgcaaatcc ggaatagcta aaaccgaaaa actaccttac
      181 gcttgtccgc cgaaaacgaa tccagaatat atacgacgag gcaattacta ttgggtcgag
      241 tgttacaaaa cccagcggga agttagctat gtttttcaat tccaattaac cggcaacggc
      301 gcaaaactaa ttagctccgg ccgaccgaca cctgagccca aaacgctgag accagcgagt
      361 gatgcaagcg acgatgcggt gaaaacggta gattaattct gttcgctgcg cttggctgct
      421 agcgttcgaa aatttctttc gcaataatca aacgctgaat ctgcgatgtg ccctcgtaaa
      481 tctggtaaat cttggcgtct cgcatgagct tttcaacagg gtactcactc gaatagccgt
      541 accctccgtg cacttgcacg gcatcggtgg caacccgcat ggccatgtcg gccgcgaagg
      601 cttttgcaaa agcggcaaat ttggtgttgc ggcggccttg gtcgatttcc caagcagcac
      661 gccagaccag catgcgggcg gcttcaatgt ctttggccat atcggcaatc atgaagctca
      721 cgccttggta agcggcgatt ggttggccga aagtctttct tgtttgggaa tagttaatgg
      781 cttcatccat ggcgcggcga gcgagtccca ccgcacctgc tgcgacgctg ggtcgggtgt
      841 gatcgaatgc gcccattgcg attttaaatc cgtcaccttc ttttcccaac aaattggtag
      901 cggggacttt gacgtcttca aacgtgatcc cgcgagtgtc acttgcgcgt tggcccatgt
      961 tccattcttt tttgccaacc gtgatacctg gtgatttggc gtcgacgata aacccagtga
     1021 gtcctttgtg ttttgcggcg gggtccgtta cggcgagtac gaaataccaa tcggccaccg
     1081 agccattagt gatccacatt ttagaaccgt tgattacgta ttcgttgcca acttttttag
     1141 cggtggtgcg aatggctgcg acatcggaac cggcattggg ttcggtcacg caataggccg
     1201 cgaaagtaaa atcagaaact aattttccta aatatttttt cttttgttca tcacttccgg
     1261 cgacgagcac cggcgcagtc gcaa
//
LOCUS       DAOMXO010000700         1283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_62710_length_1283_cov_3.306189, whole genome shotgun sequence.
ACCESSION   DAOMXO010000700 DAOMXO010000000
VERSION     DAOMXO010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62710_length_1283_cov_3.306189"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLH57_11660"
     CDS             complement(<1..538)
                     /locus_tag="PLH57_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRK03312.1"
                     /translation="MKTVVVVPTYNEALNVQPLTEALLALQVPDHEIHVLVVDDHSPD
                     GTWQIVEKMAQSNPHVHLLHRTTNRGRGLAGIEGFKWALKAGADRVVEMDADFSHHPK
                     FVPDLLAALQAGADVASGSRFVNGGSDEDGGASRQIITKMAGIYIRVLLDLPLKDASS
                     GFRAYKRHVLEAIELDRFI"
     gene            576..>1283
                     /locus_tag="PLH57_11665"
     CDS             576..>1283
                     /locus_tag="PLH57_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03313.1"
                     /translation="MDKYRFDCIHFRGYKPCAKKKVCWDCGEYSKIQKKILIIKLGAL
                     GDVMRTTPVLYAIERQNPGSHVTWVTKKNAKDMLERTPRIHRLVYVDDPDNGALLRLM
                     VEKFDEVHCYDKEDAAIGLAALVKAERRYGFEMNEWGHMAPINKASQYSFDLGLSDEM
                     KFVTNTKTYQELTYESSEFPIPSKMDHYDLQLAEADRSMANTLLNRHGFIGKKNGGAR
                     PLIGLNTGSGRVFQTKQW"
BASE COUNT          340 a          304 c          326 g          313 t
ORIGIN      
        1 aaataaaacg atcgagttca atagcttcga gcacatggcg cttgtaggcg cggaatccgc
       61 tggaggcgtc ttttagtggt aaatctagta ggacacgaat ataaattccc gccatttttg
      121 taatgatctg ccggcttgcg ccaccgtcct cgtcgctacc gccgtttaca aaacggctgc
      181 cagaggcgac atcggccccc gcctgaagcg ccgccaaaag atcgggaacg aatttcgggt
      241 ggtgcgaaaa atctgcgtcc atttcgacta cgcggtcggc tcccgccttt agagcccact
      301 taaacccttc gatgccagcc aaacccctac cccgatttgt ggtgcgatgc agcagatgca
      361 cgtgtgggtt cgattgagcc attttttcaa cgatctgcca cgtaccgtcg ggggaatggt
      421 cgtcgacgac taaaacatgg atttcatggt caggcacctg aagggccaac aaggcctcag
      481 tcaacggttg aacattaaga gcttcattat aagtgggcac gacaacaact gttttcattt
      541 tcttctgact attatagtgt ccgtctcaat tgtaaatgga caagtatcga ttcgactgca
      601 ttcattttcg tggttataag ccatgcgcca aaaaaaaggt ctgttgggac tgtggcgagt
      661 attccaaaat tcaaaaaaaa attcttatca ttaagttagg ggctctgggc gacgtcatgc
      721 gaacgacgcc ggttctgtac gccattgaac ggcaaaatcc tggttcccac gttacctggg
      781 tcactaaaaa aaatgcgaag gacatgctcg aacgcacacc gcgaattcac cgcttggtgt
      841 acgtggatga tcccgataac ggagctcttc tgcgattaat ggttgaaaag ttcgatgaag
      901 tgcattgtta cgataaagaa gatgccgcta taggattagc ggctttggta aaggctgagc
      961 ggcggtatgg tttcgaaatg aacgagtggg gccatatggc acccattaat aaggcgtcgc
     1021 agtattcctt tgatcttggt ctttcagatg aaatgaagtt cgtgacgaac accaagacct
     1081 accaggaact gacctacgaa agttctgaat tcccgattcc ctcaaaaatg gaccactatg
     1141 acttgcagtt ggctgaggcc gatcgctcta tggccaacac gctgttaaac aggcatggtt
     1201 tcatagggaa aaaaaacggg ggagcgcggc ctcttattgg cctgaacacc gggagtggtc
     1261 gagttttcca aacgaagcaa tgg
//
LOCUS       DAOMXO010000701         1273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_63447_length_1273_cov_4.399836, whole genome shotgun sequence.
ACCESSION   DAOMXO010000701 DAOMXO010000000
VERSION     DAOMXO010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63447_length_1273_cov_4.399836"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLH57_11670"
     CDS             <1..412
                     /locus_tag="PLH57_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK03314.1"
                     /translation="GVPYDRAERKHGQTHYPFKKMLKFALDGVTSFSMFPLQIATYVG
                     VASAACAFLGILYTLYLKFGTEKTIHGWTSLMIVVLFLGGVQLLAIGVIGEYLGRVFD
                     EVRRRPLYFISRSVGFDQSPVGYGEVTRQVLVRS"
     gene            complement(333..>1273)
                     /locus_tag="PLH57_11675"
     CDS             complement(333..>1273)
                     /locus_tag="PLH57_11675"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HRK03315.1"
                     /translation="VFYPVAGPFKAGSKTYSHFKSKKSAKSAWKVFFQVLREDYVLIA
                     GFLLLIQLNDDEIIMSYVFDESSPEFDQGDPDGLLVVVNTMQSSDLGHRFVKSLVDTK
                     YGHFKNRKYIEVSSYEDFLSQVEAIAATHGPITRLDLVGHGNEGAIYFGSQNMTAFDF
                     AKTRMLEGVFAKDAQVRMSTCFSGRGKNGDELLNSMAIRLLDEGGTMYAATTAYYPTD
                     QAPFMLGLGNSSRNVFRFVAKGPAALDVLLERHLGISHFGLSSYSGQPKTERTEPKVK
                     RRDYEPRAKWLKNALEPAWLLPRTLRAIDQTLPNAK"
BASE COUNT          294 a          304 c          334 g          341 t
ORIGIN      
        1 gggggtgcct tacgatcggg cggaacgcaa acacgggcaa acccactatc cgtttaagaa
       61 gatgctgaaa tttgccctag atggcgtgac gtcgttttct atgtttcctt tgcagattgc
      121 cacctatgtg ggcgtggcca gtgcagcctg cgcttttctc ggaattcttt acacgctcta
      181 tttaaagttc ggcaccgaga aaacgattca tgggtggacg tcactcatga tcgttgtgtt
      241 atttctcggg ggggttcagt tgttggcaat cggcgtgatc ggcgagtatt tgggacgcgt
      301 cttcgacgaa gtgcggcgcc gacctctgta ttttatttcg cgttcggtag ggtttgatca
      361 atcgcccgta gggtacgggg aagtaaccag gcaggttcta gtgcgttctt aagccatttt
      421 gcgcgtggct catagtcgcg tcgtttaact ttgggttccg ttcgttcagt cttaggttgt
      481 cccgaataac tggaaagtcc gaaatggctg attccgaggt gacgctccaa taaaacatcg
      541 agagccgctg gtcctttggc gacgaaacga aacacgttac gcgagctgtt gccgagcccc
      601 agcatgaagg gcgcctggtc agtaggataa taggccgtgg tggcggcgta catcgtgccg
      661 ccttcgtcta gcaggcggat ggccatcgag ttcaatagtt cgtcgccatt ttttccacga
      721 ccagaaaagc atgtgctcat gcgaacctgt gcatcttttg cgaaaacacc ttctaacatt
      781 ctggttttcg caaagtcgaa agcggtcatg ttttggctac caaaataaat cgctccctca
      841 ttgccgtgac cgacgaggtc gaggcgtgtt atcgggccat gggtggccgc gatcgcttca
      901 acctgactca agaaatcttc gtaggaagat acttcgatgt acttacggtt tttgaaatga
      961 ccgtattttg tgtcgactaa gctttttacg aatcggtgac ccagatccga gctttgcatc
     1021 gtgttgacga caacgagcag tccgtcggga tcaccctggt cgaactctgg gctagattcg
     1081 tcaaaaacat aggacataat aatttcgtca tcattaagtt ggatcagcaa caaaaacccg
     1141 gctatcaaca catagtcttc gcgcaagact tggaagaaaa ccttccacgc gcttttcgca
     1201 gattttttcg atttgaaatg cgaataagtt tttgacccgg ccttaaatgg accagccaca
     1261 gggtaaaaca cca
//
LOCUS       DAOMXO010000702         1269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_63723_length_1269_cov_5.070016, whole genome shotgun sequence.
ACCESSION   DAOMXO010000702 DAOMXO010000000
VERSION     DAOMXO010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63723_length_1269_cov_5.070016"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLH57_11680"
     CDS             complement(<1..572)
                     /locus_tag="PLH57_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03316.1"
                     /translation="MKHAYRGFRGSLTLCAVLGLMGPSLVVRAQEADPVVTPILEADP
                     APVAEAVAKSPKKKKKAKKPKKSKRNTGVASVEIPPSEPAAPDEPALKDATPQEIQKI
                     CAVMERKKRASYAKFESGKERFETERKLYMKKLEKDLNAALARPQRLFTKAIATLSEG
                     TDTSKNQVSELVEIIRAYAHPEWFEGENSSS"
     gene            complement(741..>1269)
                     /locus_tag="PLH57_11685"
     CDS             complement(741..>1269)
                     /locus_tag="PLH57_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRK03317.1"
                     /translation="NKQYTYYTGKDGVQLNGDQQNVELGYNFDCECKPRVAVEWFRAS
                     RNYRELYPTTNEFLGRADLFGRRNIMGFGIKTSGSAGDDWSWGFDYYNFSRERSSHTA
                     YRTNGTTSLGGAQLGVSRFLGHEFDLGLKYQANKNLDFKAGYNHFVSSKNSTLRKLNG
                     YTHAGMAFLAMEMKY"
BASE COUNT          299 a          344 c          290 g          336 t
ORIGIN      
        1 gatgacgaat tttcaccttc aaaccattcc gggtgagcgt aagctcgaat tatttctacc
       61 aactctgaaa cctgattttt cgaagtatct gtgccctcag acagagtcgc aatcgcctta
      121 gtaaatagcc gctgtgggcg ggccaatgca gcgtttaagt ctttctccaa cttcttcatg
      181 tacaatttgc gttcagtctc aaaccgttcc ttaccggatt cgaacttcgc gtaggaggct
      241 cgtttctttc gctccatcac agcacaaatt ttctgaattt cttgaggtgt cgcgtctttg
      301 agcgcgggct catccggcgc agcaggttca gagggtggaa tttccacgct ggctacgcca
      361 gtgttacgtt tcgatttttt cggttttttc gccttctttt tctttttggg gctctttgcc
      421 actgcctctg cgacgggcgc ggggtctgct tcgagaatcg gggttacgac cggatcagct
      481 tcctgggcgc gaacaaccag cgacggcccc atcaaaccga gaaccgcaca aagcgttaag
      541 gaaccgcgaa aaccacgata agcgtgcttc atagaccctc ctaagttgca aacgaggtac
      601 cacagtcgga gccctgcagc aaaccaggct agcctaaacg aaaagtgtct aagttttaga
      661 cggtttaaag acaaattggc aggccctcga agaagggccc acctctcccg caccacgcac
      721 cctgaagtta aggcaggtgc ttagtacttc atctccattg ccaaaaacgc cattccggcg
      781 tgggtgtacc cattcaactt gcgaagcgtg gagtttttgc tcgacacaaa gtggttataa
      841 ccggccttaa aatccaggtt tttgttagcc tggtatttca aacctagatc aaactcatgg
      901 cccaaaaacc tagaaacccc caactgcgcg ccaccaagcg aggtggttcc gttggtgcga
      961 taagcggtgt gactgctccg ttcgcgagaa aaattatagt aatcaaagcc ccagctccaa
     1021 tcatcccccg ccgagcccga cgtcttgatt ccgaatccca taatatttct tcggccaaac
     1081 aagtcggcgc gtccaagaaa ttcgttagtt gtcggataaa gttcccgata atttcgggaa
     1141 gccctaaacc actctaccgc cactctcggt ttgcattcgc aatcgaagtt gtaacctaat
     1201 tctacgtttt gttgatctcc gttgagctgc acaccgtctt ttccggtgta ataggtgtat
     1261 tgtttgttt
//
LOCUS       DAOMXO010000703         1266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_63934_length_1266_cov_4.317919, whole genome shotgun sequence.
ACCESSION   DAOMXO010000703 DAOMXO010000000
VERSION     DAOMXO010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63934_length_1266_cov_4.317919"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1266
                     /locus_tag="PLH57_11690"
     CDS             <1..>1266
                     /locus_tag="PLH57_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRK03318.1"
                     /translation="FESKAREGGGSEARARHEERGKLFVRDRVAALLDSGTAFLELSP
                     LAAHELFKGEAPGAGLVTGIGTVHGREVMIVANDATVKGGTYFPITVKKHLRAQEVAA
                     QNRLPCIYLVDSGGAFLPLQSEVFPDKEHFGRIFYNQAQMSAAGIAQIAVVMGSCTAG
                     GAYVPAMSDETIIVRKQGTIFLGGPPLVKAATGEIVTAEDLGGADVHARTSGVADHYA
                     LNDQHALEIARDIVFHLNRQSPRSADWAPRQAPEDPLYDPNEILGIVPKDARKQYDIR
                     EIIARVVDGSRMHEFKALYGTTLVTGFAHIFGYPVGIIGNNGVLFSESAVKAAHFIEL
                     CCQRRIPLVFLQNITGFMVGKQYEAGGIAKDGAKMVMAVANAQVPKFTVVVGGSYGAG
                     NYGMCGRAFNPRLLWMWPNSRISVMGGEQA"
BASE COUNT          296 a          301 c          368 g          301 t
ORIGIN      
        1 ctttgagtcg aaggcgcggg aaggcggtgg gagcgaggct cgggcgagac acgaagagcg
       61 aggaaaactt ttcgttcgcg atcgcgtggc ggcactactc gattcgggta ccgctttttt
      121 ggagttgagc cccttggcgg ctcacgaact atttaagggg gaagcccccg gcgccggact
      181 agtcacaggt atcgggacgg tccacggtcg tgaagtcatg atcgtggcga acgatgctac
      241 tgttaagggt ggcacctact ttccgattac agtaaaaaaa caccttcgag ctcaagaagt
      301 agcggcccaa aatcgacttc cctgcattta tttggttgat tcgggcggag cgtttttgcc
      361 ccttcaatca gaagtttttc cggataaaga acactttggc cgaatttttt ataaccaggc
      421 tcagatgtct gcggccggca tagcccaaat tgcggttgtg atgggctcct gcacggctgg
      481 tggggcatac gtacctgcca tgagtgacga aactattatc gtgcgaaagc agggcacgat
      541 ttttctgggt gggccaccac tcgtaaaagc cgcgactggt gaaattgtca ctgcggagga
      601 tttgggcgga gccgatgtgc acgcccgcac ttcgggagtg gcagatcatt atgctttgaa
      661 tgatcaacat gctctcgaaa ttgcacgcga catagtattt cacttaaatc gccaaagccc
      721 gcggtcggca gactgggcgc cgcgccaagc gcccgaggac ccactgtacg atccgaacga
      781 gatacttgga attgttccca aagatgcgcg caaacagtac gatattcgcg aaatcatagc
      841 ccgagtcgta gacggcagcc gtatgcacga gtttaaggcg ctatacggca cgacgctcgt
      901 cacaggtttt gctcatattt ttggttaccc tgtagggatt atcggcaaca acggcgttct
      961 gttttcagaa agtgcggtta aagcagccca ctttattgag ctttgctgcc agcgtcgtat
     1021 tcctctagta ttcttgcaaa acattaccgg ctttatggtg ggcaagcagt atgaggccgg
     1081 aggcatcgct aaagatggag cgaaaatggt gatggccgtg gccaacgcgc aagttccaaa
     1141 atttacagtt gtagtgggtg gttcttatgg agccggaaac tacggtatgt gtgggcgtgc
     1201 ctttaatcca cgattactct ggatgtggcc aaattcgcga atatcggtga tgggcggcga
     1261 acaagc
//
LOCUS       DAOMXO010000704         1263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_64167_length_1263_cov_4.951159, whole genome shotgun sequence.
ACCESSION   DAOMXO010000704 DAOMXO010000000
VERSION     DAOMXO010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64167_length_1263_cov_4.951159"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..776
                     /locus_tag="PLH57_11695"
     CDS             90..776
                     /locus_tag="PLH57_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03319.1"
                     /translation="MKKTNLLAFAVLMAASTAVGKQVETYSATENDGTPKVIRATTPT
                     LGNTMVIHLAREDRVAPPAERQRERAKELERERAAAAQSIAVKEVSEADLRDESQALD
                     EASARAESESNQSESEIDSILDEPAQKSDSPVTAEAAQPADKNVDLRLDEKRDITEKA
                     RAEAAKKAELLAKKLKFDEVPKNHSKEIELRVKYTYEILRRYGRAYDYRVLTSRELKN
                     LLASLEKTSN"
     gene            827..902
                     /locus_tag="PLH57_11700"
     tRNA            827..902
                     /locus_tag="PLH57_11700"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:860..862,aa:Phe,seq:gaa)
     gene            933..>1263
                     /locus_tag="PLH57_11705"
     CDS             933..>1263
                     /locus_tag="PLH57_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03320.1"
                     /translation="MQSKSCIRILFFLISFLALTGASPRTFTNVIPLPIYATLPQEGD
                     TFGFMPVFMVVEEPTQRTQSIYAPSYSWNKNIRHTATFRWFYYPDDETRLHLIPSISS
                     RVNRGISI"
BASE COUNT          354 a          353 c          290 g          266 t
ORIGIN      
        1 ttaaaaacag tatcaatact tgacttattc aatatgaaaa tggtatcgca ctcgagtttg
       61 aaaaagccaa tttggcggag atgggattta tgaaaaaaac aaatttgctc gctttcgctg
      121 tgctcatggc agcgtcaacg gctgtcggaa aacaggttga aacctatagt gccaccgaaa
      181 acgacggcac tccaaaagtg atccgtgcca ccactcctac ccttggcaac acgatggtca
      241 ttcacctcgc acgagaagat cgagtcgccc ccccagccga acggcaacgc gagcgagcta
      301 aagaacttga acgcgagcga gctgcagccg cgcaatcgat cgcagtcaaa gaagtcagcg
      361 aagccgacct ccgagacgaa tcacaagccc tcgatgaagc tagtgcccgc gccgagtccg
      421 agtctaacca gagtgaatcc gaaattgact cgattcttga tgaacccgct caaaaatctg
      481 actcgccggt aacggcggag gccgcacaac cggccgacaa aaacgtagac ctacgcctcg
      541 acgaaaaaag ggacatcaca gaaaaagctc gtgctgaggc ggcaaaaaaa gcggaacttc
      601 tcgcaaaaaa acttaagttc gacgaagtcc caaaaaatca cagtaaagaa atcgagctgc
      661 gcgtgaaata cacctacgaa attttacgac gctatggccg ggcctatgat taccgggtct
      721 tgacctcgcg cgaactaaaa aaccttttgg cgagtctcga aaaaacctcg aactaaagtc
      781 cttgcgtttc tgtttttcgc gtggcaccta tcgaaatcta gccgttgccc aggtagctca
      841 gtcggtagag cagcgccctg aaaaggcgcg tgtcggcggt tcgattccgt ccctgggcac
      901 cacttgaatc agagaacgac cctcaccctc gcatgcagag caagtcttgt atccgaatat
      961 tgttttttct gatttcgttt ctagcgctta cgggagcgtc gccccgaacc ttcaccaatg
     1021 ttattccgct acccatttac gccactcttc cccaagaggg cgatactttc ggattcatgc
     1081 cagttttcat ggtggtcgag gaacccactc agcgaactca gagtatctac gcacccagtt
     1141 acagctggaa taaaaacatt cgccacaccg ccacctttcg atggttctat taccccgacg
     1201 acgaaactcg gctacaccta attccgtcga ttagctcgcg ggttaaccgg ggcatttcca
     1261 ttg
//
LOCUS       DAOMXO010000705         1259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_64462_length_1259_cov_3.677741, whole genome shotgun sequence.
ACCESSION   DAOMXO010000705 DAOMXO010000000
VERSION     DAOMXO010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64462_length_1259_cov_3.677741"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLH57_11710"
     CDS             <1..266
                     /locus_tag="PLH57_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03321.1"
                     /translation="GDLGKHVIDYTVVGGMQAFTLFDGTLEQTKKFLFKLWDLGAIAF
                     FCGHDPYRVRMLPPLAVMTEAHVKEVFTLIETAVRETAKEIQR"
     gene            263..>1259
                     /locus_tag="PLH57_11715"
     CDS             263..>1259
                     /locus_tag="PLH57_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRK03322.1"
                     /translation="MNFLEQVENIIRINSVSQNGNEEIAVYLQSLMDKMGFKVRLQEV
                     SHSLEGVSKRQFNLIGTLGDPLVDTKTKKGLLLNTHIDTVGPGLKSNWTETSGDPFAA
                     TIKDGKIFGLGSADVKLDFLCKLKACERYKDRKLKMPIYLVGTAGEEIGMIGAKFLIK
                     SLAVNPKYVVVGEPSELSIIYAHKAYCVYQVSVGYAQLERDAKGFNTRIGLQCFGKSA
                     HGSYPHLGENAIQKMLQVIEKIRAANFQMRFTRTLGGDAVNKVPDSAAIEFYLQSSSF
                     DDFRRFYRDQLSQDNVRVEFGGLGESGVKFLPDSVFEVVEGINRAVVQVQTDFLTA"
BASE COUNT          363 a          252 c          311 g          333 t
ORIGIN      
        1 aaggtgattt gggaaaacat gttatcgact acactgtagt aggaggcatg caagcgttca
       61 cgctctttga cggcacgctc gaacagacca aaaaattcct ctttaaactt tgggatctcg
      121 gcgcaattgc tttcttctgc ggccacgacc cataccgggt acgaatgctt cctccgctgg
      181 ctgtaatgac agaggctcac gtaaaagaag tttttacatt aattgaaaca gcagttcgtg
      241 aaactgcaaa agagattcag cgatgaattt tttggaacaa gttgaaaaca ttattcgaat
      301 taattccgta agccaaaatg gcaacgaaga gattgccgtt tatttgcagt cgctcatgga
      361 caagatgggc tttaaagttc gacttcaaga ggtgagtcat tctcttgagg gagtaagtaa
      421 acgccaattt aatttaattg gtaccctcgg tgacccgttg gtggacacca aaactaaaaa
      481 agggttgttg ctcaataccc atatcgatac ggtgggtcca ggcctgaaaa gcaattggac
      541 agagacttca ggcgatccgt tcgctgccac aataaaagac ggtaaaattt ttgggctagg
      601 atcagccgac gtaaaattag attttctctg caaattaaag gcctgcgaac gatataaaga
      661 tcgcaaacta aaaatgccaa tttatcttgt gggcactgcc ggcgaagaaa ttggtatgat
      721 cggcgctaaa tttttaatta agtctctggc tgtaaatccc aaatacgttg tagtgggcga
      781 gccgagtgag ttgtcgatta tttatgccca caaagcctac tgcgtttacc aagttagtgt
      841 ggggtatgcg caactcgaac gagatgcaaa aggtttcaac actcgaattg ggttacagtg
      901 ttttggaaag tctgctcatg gcagttatcc gcacctgggt gaaaatgcga ttcagaagat
      961 gcttcaggta attgaaaaaa ttcgagccgc gaattttcaa atgcggttca ccagaaccct
     1021 ggggggcgac gcggttaaca aggttcctga tagcgcggct atcgaatttt atttgcagtc
     1081 gagcagcttt gacgatttta gaagatttta tcgcgaccag ctttcgcagg acaacgtaag
     1141 agtcgaattc ggtggtctcg gcgaaagtgg cgtaaagttt ttaccggatt cagtattcga
     1201 agtggttgaa ggaattaatc gcgccgtagt gcaagtgcag acagatttcc tcacggccc
//
LOCUS       DAOMXO010000706         1255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_64764_length_1255_cov_4.633333, whole genome shotgun sequence.
ACCESSION   DAOMXO010000706 DAOMXO010000000
VERSION     DAOMXO010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64764_length_1255_cov_4.633333"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PLH57_11720"
     CDS             complement(<1..1244)
                     /locus_tag="PLH57_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRK03323.1"
                     /translation="MDLYNLIGFDAAAIGNHELDYGPAVEGRIDVKKGEDGLGNLKKR
                     VKEAKFAWLSANLIQKNAPSCKPGPRCNALGQRTVFAPRAYFHKNETKTCVIGATTPS
                     TEHITVVDFVKNVRFKALYSVVLAESKYLRTSQRCDFVVLVAHAGLLCNKEGRCRERG
                     DRAEILDLLEKLPQGTLDAVVAGHTHLKAQEVIHGTPVIEAGSYARTVGVLKLWKKNR
                     RHEFASWSEVPTKANEPAAVLDITNAMARYRDNAEDLKRLPVGESEDRFEKVHTTELA
                     LGNLVADALLEYARTIDGDVCCAFVNQGGLRADLPEGKLNYGDVFRVLPFDNSLAIAS
                     LTGEELFELLRIGTSGGHGIFPVAGLSLTRTAAPPGMRGRWDRDLDSNGVSEDWERDL
                     LLDATLTPEGRMIDPRKRYKIAT"
BASE COUNT          289 a          334 c          310 g          322 t
ORIGIN      
        1 gtggcaattt tataacgttt ccgagggtca atcattcgcc cctcgggtgt tagcgttgcg
       61 tcaagcaaga gatcccgctc ccagtcttcg gacactccgt tcgaatccag atcgcgatcc
      121 cagcgacctc gcataccggg tggtgccgca gttcgtgtca gtgagagtcc agctaccgga
      181 aaaatcccgt gtccaccgct agtacctatt cttaatagtt cgaaaagctc ttctccagtg
      241 agggaggcaa tagccaaact gttgtcgaac ggcagaactc gaaacacgtc gccgtagttt
      301 aacttgcctt ccggaagatc tgctcggagt cccccttggt taacaaatgc acagcagaca
      361 tcgccatcaa tcgtgcgggc gtattcgagc aaagcgtcgg caactagatt gccgagagca
      421 agttccgttg tgtgcacttt ttcgaagcga tcttctgatt caccaaccgg gagccgctta
      481 agatcctcgg cgttgtctcg ataccgagcc atggcattgg taatgtccaa aacggctgct
      541 ggctcgttgg ctttcgtcgg tacctcggac caagatgcga attcgtggcg gcggtttttc
      601 ttccaaagct ttagaactcc aacggtgcgg gcgtaagaac ctgcttctat gaccggggtt
      661 ccatggatca cttcttgagc tttcaaatgg gtatggccag caactactgc atcaagagtg
      721 ccttgaggaa gtttttctag cagatcgagg atctcggcac gatcaccgcg ttcccgacaa
      781 cggccctcct tattgcaaag cagacccgcg tgggccacca gcactacaaa atcacaacgt
      841 tgactggttc gcagatactt ggattcggcc aagacaacgc tgtagagcgc tttaaaacga
      901 acatttttta cgaagtcgac gacagtaatg tgttcggtgc ttggtgtagt ggcaccgatg
      961 acacaagttt tggtttcgtt tttatgaaaa taagcacgcg gagcaaacac cgttctttgt
     1021 ccgagcgcgt tacagcgtgg acccggtttg caacttggcg catttttttg aatcaaattt
     1081 gccgaaagcc aggcgaactt ggcttctttg actcgttttt ttaaatttcc aagaccgtct
     1141 tcgccttttt ttacgtcgat acgcccctca actgccggcc cgtaatcgag ctcgtgattt
     1201 ccgatggcgg cagcatcgaa tccaatcagg ttatataaat ccactaccgc gcgac
//
LOCUS       DAOMXO010000707         1252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_65028_length_1252_cov_2.895572, whole genome shotgun sequence.
ACCESSION   DAOMXO010000707 DAOMXO010000000
VERSION     DAOMXO010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65028_length_1252_cov_2.895572"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..330)
                     /locus_tag="PLH57_11725"
     CDS             complement(76..330)
                     /locus_tag="PLH57_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRK03324.1"
                     /translation="MQIPYPWPYHRLMDGEKKNVPLVCFCHCVPEAVIREAIRAGART
                     IEKIQAETQASTGCGGCHDEVQAILADELEKLKISESKAG"
     gene            318..860
                     /gene="folE"
                     /locus_tag="PLH57_11730"
     CDS             318..860
                     /gene="folE"
                     /locus_tag="PLH57_11730"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006276486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRK03325.1"
                     /translation="MESAIRSLLLELGENPDREGLQKTPARLAKALKFLTSGAKTDPR
                     DLLKSALFSEEYSEMVLVKDIEVFSMCEHHMLPFYGKAHVAYIPDGKIVGLSKLARVV
                     DVLARRLQVQERLTHQISEAINDVLKPKGVAVVVQANHLCMMMRGVEKQNSSTVTSSM
                     LGAFRDDVRTRSEFLKLISV"
     gene            857..>1252
                     /locus_tag="PLH57_11735"
     CDS             857..>1252
                     /locus_tag="PLH57_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03326.1"
                     /translation="MTDTSSSDKTPILLHPARRLELLRLGQEHFNNDRFGDAIEMWHQ
                     LLNVEQGRDKEFVKMLLDCAGHFADLKKSAWDAAEKMAQATFEKLSLPAAHGAYRDLD
                     LAPVHSALDYNLGLLRTTANKNILVAESFI"
BASE COUNT          326 a          300 c          304 g          322 t
ORIGIN      
        1 ctgctcgagc accgactgat cacctgaacg cgatcttaga gtcgactgcc cccgcccaag
       61 ggtgcccgta agtcgtcaac ctgcttttga ttcagaaatt tttaattttt cgagttcatc
      121 ggcgagaatg gcttgtactt cgtcgtgaca gccgccacac ccggtactgg cttgagtttc
      181 ggcttgaatc ttttcaatcg tgcgggcgcc cgcccgaatg gcctcgcgaa taacagcttc
      241 aggcacacag tgacagaaac agaccaaggg gacattcttt ttttcgccgt ccatcaatct
      301 atgataaggc caggggtatg gaatctgcaa ttcgttcttt attgttagag ctcggcgaaa
      361 accctgaccg ggaggggctg caaaaaactc cagcacggtt ggctaaggca ttgaagttct
      421 taacttcagg ggcaaagaca gaccctcgtg atctcttaaa atctgcgctc ttttcggaag
      481 aatattccga gatggtgctg gtcaaagaca ttgaagtgtt ttccatgtgt gagcaccata
      541 tgttgccgtt ttacggaaaa gctcacgtag cctatattcc cgatggtaag atcgtaggac
      601 tctcgaagct ggcccgagtg gtcgacgtac ttgcacgacg acttcaagtg caagagcgcc
      661 tgacccacca aatttctgaa gcaatcaacg acgtgctcaa gccaaaaggc gtggccgtgg
      721 ttgtgcaagc caatcatttg tgtatgatga tgcggggagt ggaaaaacaa aactcctcga
      781 ccgtgacttc tagcatgttg ggtgcgtttc gagatgatgt tcgaactcgc tccgaatttt
      841 taaaattaat atcagtatga cggacacttc ttcgagcgac aaaacaccga ttcttctaca
      901 ccctgccaga cggctggaac ttttgcgttt gggacaagag cacttcaata acgatcgttt
      961 tggagatgcc attgaaatgt ggcaccagct actgaatgtt gaacaaggtc gcgacaaaga
     1021 atttgtaaaa atgttgctcg actgcgctgg tcattttgca gatttaaaaa aatcggcatg
     1081 ggatgccgca gaaaaaatgg cccaagcaac attcgaaaaa ctatcgcttc cggcagccca
     1141 cggggcttat cgcgatttag acctggcgcc tgtgcattcg gcactcgatt acaatcttgg
     1201 tttgcttcga accaccgcca ataaaaatat tttagtagca gagtctttta tt
//
LOCUS       DAOMXO010000708         1248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_65305_length_1248_cov_4.040235, whole genome shotgun sequence.
ACCESSION   DAOMXO010000708 DAOMXO010000000
VERSION     DAOMXO010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65305_length_1248_cov_4.040235"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PLH57_11740"
     CDS             complement(<1..961)
                     /locus_tag="PLH57_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK03327.1"
                     /translation="MFSRSSHCVQEIKRGLGYLAFAVTVSFIAAAPAAAYSEAQFRAV
                     TTENEAQINEIREQEINQIKVVMGRRFSESRRPDILLRLAELYIERYRFFFFKENEIH
                     QAQLKSGLRPKRVDHTRSRAALKSAENACLSILKSKVPFAKMDQVYYFLAYNAQELGK
                     QNEALRYFETIVKRYPRSQYAAETYRNLGEAEFGKKNYKKSVQYYEKAVRYTELESYP
                     RTLYKLAWSYFKTRDKTRALAAMKQVVARSQEDDKFIALKEEALNDLVMFYAEAGRAK
                     EAHEYFSQVKGGPELYVKALNRLATLYGTQGRHEMSLYINEELI"
     gene            complement(924..>1248)
                     /locus_tag="PLH57_11745"
     CDS             complement(924..>1248)
                     /locus_tag="PLH57_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03328.1"
                     /translation="EDVLNWRLKSIRLVGGLFVKNATADIYRDLLSNLDKMSFIKLEM
                     LKYAKGRLERTQTMSADDDGVMKRGMADIDRKDYQYFWTFNGEFWADELGDYVFALES
                     LCSRN"
BASE COUNT          295 a          311 c          285 g          357 t
ORIGIN      
        1 gaatcaattc ctcatttata taaagtgaca tttcgtggcg cccttgggtg ccatagaggg
       61 ttgccaaccg atttaatgcc ttaacgtaga gctcgggacc accctttacc tgagaaaagt
      121 attcatgagc ttcttttgcg cgcccggctt ctgcgtaaaa catcactaag tcgttgagcg
      181 cttcttcttt gagagcaatg aatttatcgt cttcctggct gcgcgctacg acctgtttca
      241 ttgctgctag tgcacgagtc ttgtcgcggg tcttgaaata ggaccacgcc aacttgtaca
      301 aggtacgcgg gtagctttcc aattcggtgt agcgtacggc tttttcgtag tattgtactg
      361 attttttgta gttctttttt ccaaactcgg cctcacccag atttcggtag gtttcagctg
      421 catactgact tcttggatat cgcttcacta tggtttcgaa atatcgaagc gcttcgtttt
      481 gcttgccaag ttcttgggcg ttgtatgcga ggaagtaata cacctggtcc atttttgcga
      541 aaggcacttt agatttcaaa atcgaaagac aggcgttctc ggcagatttc aacgccgcgc
      601 gcgatctggt atgatccacc cgtttcggtc gtaaaccact tttgagctga gcttggtgga
      661 tttcgttctc cttaaaaaag aagaaccgat aacgttcgat atagagttcg gctagccgca
      721 agagaatgtc gggtcgacgt gactccgaga accggcgtcc catgacaacc tttatttgat
      781 tgatctcttg ttcccggatt tcgttaatct gagcctcatt ttcggtggta acagcccgaa
      841 actgcgcctc gctataggct gcggcgggcg cagctgcgat gaacgaaaca gtgacagcga
      901 aagctaggta tccgagccca cgcttaattt cttgaacaca atgactcgag cgcgaaaaca
      961 tagtccccca actcgtcagc ccaaaactct ccgttgaacg tccagaaata ctgataatcc
     1021 ttgcgatcaa tgtccgccat gccccgcttc atgacaccat cgtcgtcggc actcatggtt
     1081 tgagtacgtt ccagacggcc tttcgcgtat ttgagcatct cgagtttaat gaatgacatt
     1141 ttatctagat tcgaaagtag atcgcgataa atatcggccg tggcattttt aacaaataat
     1201 ccgcccacaa gccgaatact ctttagacgc cagttcaata cgtcctcg
//
LOCUS       DAOMXO010000709         1245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_65537_length_1245_cov_4.289916, whole genome shotgun sequence.
ACCESSION   DAOMXO010000709 DAOMXO010000000
VERSION     DAOMXO010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65537_length_1245_cov_4.289916"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PLH57_11750"
     CDS             <1..1086
                     /locus_tag="PLH57_11750"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRK03329.1"
                     /translation="TAYGRLDEAVEAMRLGALHFLSKPIRKKDLEVLIHDVGREVGSK
                     AKDSTRGHPHEPVVVSRTFAEVIQTIDRVAPNLASVLLVGESGVGKEVLARRLHAKSA
                     RTSAPFVAFHAGAVPESLVESELFGYVKGAFTGADQDKPGLLTQANGGTFFLDEIATM
                     PVGVQAKLLRVIQERMVQPLGATTAHPIDVRWVAATNANLKTEAERGGFRQDLLYRLS
                     VVEVEVPPLRTRVEDILPLTQAFLEEFSRREGRAVLTLNPNVAEKLKAYPWPGNVREL
                     RNVVERAVALATADQFDVALLPEQVNSAEKSREVRIAVGTTLQSAEDKLIEETLRMCG
                     GDKVQAAAILGVAPRTIYRWLEKRTQV"
BASE COUNT          308 a          288 c          336 g          313 t
ORIGIN      
        1 accgcctatg gtcgcctcga tgaagccgtt gaagccatgc ggcttggagc tttacatttt
       61 ctttcaaagc ccattcggaa aaaagaccta gaggttttga ttcacgacgt tggtcgtgaa
      121 gttggatcca aggcaaaaga ctccacgaga ggacacccgc atgaaccagt ggtggtgtcg
      181 cgcacctttg cagaggtgat tcaaaccatc gaccgcgttg ctcccaatct agcgtcagtg
      241 ttattggttg gagaaagtgg cgtaggaaaa gaagttcttg cgcgaaggct gcatgccaaa
      301 agtgcacgaa ccagcgctcc gtttgttgca tttcacgccg gtgccgtacc ggaatctttg
      361 gttgagagtg aactttttgg ttacgtaaaa ggggccttta ccggagccga tcaggacaag
      421 ccgggactgt taactcaagc gaatggcggt actttttttc tcgatgagat tgccaccatg
      481 ccggtgggag tgcaggcgaa gctcctaaga gtgatccagg aacgaatggt gcaaccgctc
      541 ggagccacca ctgcccaccc gatagatgtc cgttgggtgg cagcgaccaa cgccaatctc
      601 aaaactgaag ccgagcgggg tgggtttcgc caagatctat tgtatcgcct aagcgttgtg
      661 gaagtcgaag tgcctccgct tcgcacccgc gttgaagata ttctcccgct cactcaagca
      721 tttttagaag agttttcacg ccgagaaggg cgagcggtgc tgacgttaaa tccaaatgta
      781 gccgaaaaac taaaggccta cccttggccc ggcaacgtgc gtgaacttcg taacgtggta
      841 gaaagggctg tagctttggc gactgccgat cagtttgatg ttgcgctttt gccggaacag
      901 gtaaactcgg cagagaaatc gcgcgaagtt cgaattgcag taggtacgac tcttcaatcg
      961 gctgaagata aactcattga agaaacacta cgcatgtgtg gcggcgacaa ggtacaggca
     1021 gcagcgattc ttggtgttgc tccccgaact atttatcgat ggttggaaaa acgcactcaa
     1081 gtttaaaaaa cttcttcttt actcgtgtgt gtaactggag cgttttgtaa ttctcgtagt
     1141 agatccaaat acagcttttt tgcgttttgc tgtgagatgc gatccccatt caaggttgta
     1201 gtataaattt tttgaatgaa ctcgagtttc gttttacccg aagcg
//
LOCUS       DAOMXO010000710         1244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_65607_length_1244_cov_5.224558, whole genome shotgun sequence.
ACCESSION   DAOMXO010000710 DAOMXO010000000
VERSION     DAOMXO010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65607_length_1244_cov_5.224558"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLH57_11755"
     CDS             complement(<1..474)
                     /locus_tag="PLH57_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRK03330.1"
                     /translation="MDRFALNSIKRSSLALLVTVAAGLFALPLQAAQDSTVIEENAPV
                     HAEPAANAKVIDYLPLGAEIRISSYPLPGGWYKVRARNGIYGWVHENHVSVEKETETK
                     PRVEVIEGPQPERDRKWFIRALGGYDFFRPNDLNDAFRFNDLNTGNTIGGELGYFI"
     gene            complement(456..>1244)
                     /locus_tag="PLH57_11760"
     CDS             complement(456..>1244)
                     /locus_tag="PLH57_11760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRK03331.1"
                     /translation="QSDGNVPLAEVSGIFRDAKVEFIPLTNHQFGAFVGVEYGMDPGM
                     VPLELKYRFQDETEFEKYNFEVSVVASVFPSEKLRVPPRTIAPSAKDRVRIQRDNEAI
                     RRAYATVTMKRFWSDSPVLPVNSQVTSVFGSSRVYNGKKTSAHLGTDLRAPTGTRIVA
                     PTGGQVVLVRDTFYLGKTIIVDHGFGVFTIYGHLSKTLVKPGKVVKTGQLLGLAGATG
                     RVSGPHLHWAAHLNGVKVDPLLLMSAMETSLGKKSKESRWIASP"
BASE COUNT          306 a          334 c          298 g          306 t
ORIGIN      
        1 gataaaataa ccgagttcgc caccaatggt gttgcctgtg tttaggtcgt taaatcgaaa
       61 ggcgtcgttg agatcgtttg gtcgaaaaaa atcgtacccc cccaaggctc gaataaacca
      121 cttgcgatcg cgctccggtt gaggaccttc gatgacttct acgcggggtt tggtttccgt
      181 ttctttttca acagacacat gattttcgtg cacccaacca taaatcccat tacgcgcccg
      241 caccttgtac cagccaccgg gcaaagggta gcttgagatt cgaatttccg cccccagcgg
      301 taaatagtcg atcaccttgg cattggcagc cggttcggca tgcactggtg cattctcttc
      361 gatcacagtg gaatcctgcg ctgcctgcag aggcaaagca aagagccccg cagcgacggt
      421 caccagaaga gcaagagaac ttcgtttgat tgaatttagg gcgaagcgat ccatcggctt
      481 tctttcgact tcttaccaag cgatgtttcc atagcactca tcaacaacag cgggtctact
      541 ttaacgccat ttaagtgcgc agcccaatgc aggtgaggtc cactcactcg accggtggcg
      601 cccgcaagcc ctaacaattg gcctgtctta acgaccttgc ccggctttac taatgttttc
      661 gataaatggc cgtagatcgt aaagacgcca aacccgtgat ctacgataat ggttttgccc
      721 aaataaaagg tatcccgaac caatacaact tgtccgcccg tgggcgcgac gattcgggtt
      781 cccgtcgggg cacgaagatc cgtgcctaag tgagcactgg tttttttgcc attgtacacg
      841 cggctagaac cgaaaaccga agtcacctgg ctatttacgg gaagcaccgg ggaatcactc
      901 caaaatcgct tcatggtaac cgtagcgtat gcgcgtcgaa ttgcttcatt atcgcgctga
      961 atgcgcacgc ggtccttggc cgagggtgca atggttcgag gcggcactct tagtttttca
     1021 gatggaaaca ctgaagcaac aacagacact tcgaaattgt atttttcaaa ctcggtttca
     1081 tcttggaatc ggtattttag ttccaaggga accattcccg ggtccatgcc gtattccacg
     1141 cccacaaacg cgccaaactg gtggttcgtc agcggaataa attcaacttt tgcatctcga
     1201 aaaattccag acacttcagc gagcggtaca ttgccgtctg attg
//
LOCUS       DAOMXO010000711         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_65760_length_1242_cov_3.684920, whole genome shotgun sequence.
ACCESSION   DAOMXO010000711 DAOMXO010000000
VERSION     DAOMXO010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65760_length_1242_cov_3.684920"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..795)
                     /locus_tag="PLH57_11765"
     CDS             complement(166..795)
                     /locus_tag="PLH57_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRK03332.1"
                     /translation="MPEGLTRRHLHLLANSRSGKGLGSTLAERARKLCSELGADLTIY
                     DIKSPEQLTDYAKKAIAIAQNSPHHVVIAAGGDGTIRSVAAEAAGTKAKLAVVPCGTF
                     NFFARTHSIPEDQLEALRLAIVGGCKPIRLGDINGHKFLINASLGLYAQSIREREQAT
                     KTFGRKQLVVILSTIRSLLQRHRLLHVRLKNDNFKSEIKTPMICWRSLC"
     gene            1032..>1242
                     /locus_tag="PLH57_11770"
     CDS             1032..>1242
                     /locus_tag="PLH57_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRK03333.1"
                     /translation="MDDWEIELKAGFLEEASQAISDNEQNFLELEKDPSNTDLVDKIF
                     RLAHNLKGSAKAVGFSELGAFTHEFE"
BASE COUNT          349 a          261 c          282 g          350 t
ORIGIN      
        1 agttgatata gaacatttct ccatctaagg ccaccatctg tggactacgt ttagtagtga
       61 taattagatt gtcggtcgag aactggtcta agcgttcttc tttttctaac gtttttaaaa
      121 taccccgaaa aattacccgc aacatttccc agccgcggac gggtttcagc ataatgaccg
      181 ccaacaaatc atgggggttt ttatctcact tttaaaatta tcgtttttta aacgaacatg
      241 cagcaatcga tgacgctgta aaagggaccg aatcgtagat agaattacga ccaactgctt
      301 tcgtccaaac gtcttagtcg cttgttcgcg ctctcgaatg gattgagcgt aaagccccag
      361 gctcgcgtta atcaaaaatt tgtgaccgtt gatatcacct aggcgaatgg gtttgcagcc
      421 accgacgatc gccaaacgga gggcttctaa ttgatcttcg ggaatcgagt gggtgcgtgc
      481 aaaaaagtta aaggtcccgc agggaaccac agccaatttg gcttttgtac ccgcggcttc
      541 ggctgccaca cttcgaatgg tgccatcgcc cccagcggct ataacaacat gatgtgggga
      601 attttgggcg atcgctatgg cctttttggc ataatcggtg agctgctcag gggacttaat
      661 atcgtaaata gtaagatcag cgcccaattc ggaacataat ttccgagccc gctcggccag
      721 ggtagaacca agtcctttgc ctgagcgtga attggctagt agatgaagat gtcggcgcgt
      781 taagccttca ggcattacca ttcctatttt tatatttctc ttgggttgtc tagcgcgatc
      841 tcaagaaacc ttgaggttgt aaaaaacgac gcagaccata gcccaaaaaa aatgtaatca
      901 ttcaaatcga tacagttttt ttatacgctt cgagatggag atttcttcat cttcagtaag
      961 tatctgaaat tattagataa tatttaattg cataatcttt aggattttct attcgggccg
     1021 aaacgtaaga tatggatgat tgggaaatag agttaaaagc cgggttcctt gaagaagcgt
     1081 cgcaagcgat tagtgacaac gagcagaact ttttggaatt agaaaaggat ccttcaaata
     1141 cagatctagt agataagatc tttcgattag cccacaacct taaaggtagt gcaaaggccg
     1201 tcggtttttc agagctcgga gcctttactc atgaatttga gt
//
LOCUS       DAOMXO010000712         1241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_65820_length_1241_cov_5.241990, whole genome shotgun sequence.
ACCESSION   DAOMXO010000712 DAOMXO010000000
VERSION     DAOMXO010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65820_length_1241_cov_5.241990"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..>1241)
                     /locus_tag="PLH57_11775"
     CDS             complement(173..>1241)
                     /locus_tag="PLH57_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRK03334.1"
                     /translation="MRLLLKNFKSNDGAKLAFFFLTIGLTGFIFFVTPDLMPSSLLSI
                     LVYFIFSPVIDGFERRGVSRTNAVIGFFLLSGVALAAVVTWGIPMATEEFRSFNAGSG
                     KTTSILYDRLHEQEKALAEKIPSLKSFSITQHFAEWISQSAKSLWTGLSRIFSQVLLV
                     SFLVPFFSFVLLRDGHEIRRSLLRMVPNRFFETVYSLSYRIIDKMGGYVSARIIEAAA
                     VTAMVTIGCLIFGVPYAILIGLFAGATNPIPYLGPVLGAIPALVLALLEPAVPNQLAI
                     VGMILLIANLVDMFLIFPFLVARIVDLHPVVVVVCVIIGSKVFGVVGMIVAVPIASIL
                     KILIEEFYTKVYNQRADSPLR"
BASE COUNT          396 a          272 c          308 g          265 t
ORIGIN      
        1 aaattaaagg gaagtcatgc gttttttctt cgtcatcacc attttgtttc tggtcgctag
       61 tctcgcgaaa gcgtggtcgt tttaacggga cagacgaaat ccattaggca tctgggttgg
      121 gcgccaggcc tggcgccaat aggtgtcggt gcgagttagc cacttgcgaa aatcaacgaa
      181 gcggcgagtc tgcgcgttgg ttgtagacct tggtgtaaaa ctcttcgatt aaaattttca
      241 gaatagaagc aatcggaacg gctacgatca ttccaactac gccaaacact ttcgaaccaa
      301 taatcacaca aacaacaacg acgaccgggt gaaggtctac gattcgagcg accaaaaaag
      361 gaaaaattaa aaacatatcc acgaggttgg cgatgagtaa gatcatgcca acaatagcga
      421 gctggtttgg aacggcaggt tctagcagtg ccagcacaag agcggggata gcgcccagca
      481 caggaccgag atacgggatt ggattggtgg cgcccgcgaa aagtccgatc aaaatagcgt
      541 aaggcactcc aaaaattaaa cagccgatcg ttaccatggc agtgacagcg gcggcttcaa
      601 taatgcgcgc ggaaacgtag ccacccattt tgtcgataat acggtaagag agcgaataga
      661 cagtttcgaa aaaacggttc ggaaccatac gtagtaaaga tcggcgaatt tcgtgaccat
      721 ctcgcagtag aacgaagcta aaaaacggaa ctagaaacga caccagtaga acctgggaaa
      781 aaattctaga aagtccggtc caaagactct ttgcactctg ggaaatccac tcagcgaagt
      841 gttgggtaat tgaaaaggac tttagtgaag gaattttttc ggccaaggct ttttcttgct
      901 cgtgaagccg gtcgtataga atggaagtgg ttttgccact gccagcattg aaagagcgaa
      961 attcttcggt agccatcggg attccccagg tcacgaccgc tgcgagtgca acaccgctga
     1021 gtaaaaaaaa tccaatcacg gcgttggtgc gcgacactcc acgacgttcg aatccgtcaa
     1081 ttaccggaga aaaaataaaa tacaccaaaa tacttagaag cgaacttggc atgagatccg
     1141 gagttacgaa aaaaataaag cccgtcaatc cgatggtaag aaagaaaaag gccaactttg
     1201 caccatcgtt ggacttaaaa tttttcaaaa gtagacgcat t
//
LOCUS       DAOMXO010000713         1240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_65958_length_1240_cov_2.570464, whole genome shotgun sequence.
ACCESSION   DAOMXO010000713 DAOMXO010000000
VERSION     DAOMXO010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65958_length_1240_cov_2.570464"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1240)
                     /gene="bamA"
                     /locus_tag="PLH57_11780"
     CDS             complement(<1..>1240)
                     /gene="bamA"
                     /locus_tag="PLH57_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRK03335.1"
                     /translation="NNQRFPDERLKAVLRNTKEGNFFSWISSTGNFKESAFKQDMQML
                     TYYYLNEGYVKFRYENPVVTVSEDKRWLFISIYVDEGERYTVGEIDFGGDLLFDKSDL
                     AQTTEMKSGQFFSIQKRNDDIQKLTEKYQDLGYAFVNVIPKMNIRDDVRLVDIRYDFE
                     KGDIVHFGEINVLGNSKTRDKVIRRELRVREGEKYHGTRLRLSRERVERLGFFNPGEV
                     VFNTVTRKDKKDVVDLEIGIKERSTGTVSLGMGYGSIQKFFLTGSIAETNLFGRGQNL
                     SLSLQYAADRKSRSLSLGFTEPYLMNSRWSAGFDVFLTSFVIPDRYLLFKKGFDLRFG
                     HPLDRIDEDMYAYLNYKLETLELDEIDSRFPDLNANDDRGSLSAIGMSVVRDKRNNRF
                     EPTKGNYESLGGEFAGVGGKK"
BASE COUNT          329 a          308 c          242 g          361 t
ORIGIN      
        1 tttcttgccg ccaactccag caaattcccc cccaaggctt tcataattcc ctttggtggg
       61 ctcgaatcta ttgtttcgtt tgtctcgaac taccgacatg ccgatagccg acaaagagcc
      121 cctatcatcg tttgcattaa gatcaggaaa tctcgaatcg atttcgtcta actccaacgt
      181 ctcgagtttg taattgagat aggcgtacat gtcttcgtcg attcgatcga gtgggtgccc
      241 gaagcgcaga tcaaatccct ttttaaataa caagtagcga tcgggaataa cgaaactcgt
      301 taaaaatacg tcaaatcccg cgctccacct tgaattcatt aaatagggtt cggtaaagcc
      361 caagctcaaa gagcgcgact tgcgatcggc cgcgtattgc aacgacaacg ataggttttg
      421 accgcggccg aaaagattcg tttccgctat agaccccgtt aagaaaaact tttgaatact
      481 accgtagccc atacccaaag atacggtccc ggttgatctt tctttgattc caatttccag
      541 atcaactacg tcttttttgt ctttacgagt taccgtatta aatacaactt cgccggggtt
      601 aaaaaagcca agacgctcaa ctcgttcgcg gctcaacctt aagcgagtcc catggtattt
      661 ttcgccttca cgaacacgaa gttcccggcg aattacttta tctcgtgttt tgctgttacc
      721 aagtacatta atttcgccga aatgtactat gtcacctttt tcaaaatcgt atcgaatatc
      781 gaccaaacgc acgtcatcgc gaatattcat cttcggaatc acgttcacga atgcatatcc
      841 cagatcttgg tacttttccg tgagcttttg aatgtcgtcg tttctttttt gaatcgaaaa
      901 aaactgacca gacttcattt cagtggtttg ggcaagatca cttttgtcaa acagaaggtc
      961 gccgccgaag tcgatttccc cgaccgtgta gcgctcccct tcgtctacgt aaatcgaaat
     1021 aaagagccag cgtttgtctt cgctcaccgt taccaccgga ttttcgtagc gaaatttaac
     1081 atacccctca ttgaggtagt agtaggtgag catctgcatg tcttgtttaa acgcagattc
     1141 tttgaaattc ccggtcgaag aaatccagga aaagaaatta ccttctttag tattgcgcag
     1201 aactgctttt aatcgctcat ccgggaaccg ttgattatta
//
LOCUS       DAOMXO010000714         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_66129_length_1237_cov_5.046531, whole genome shotgun sequence.
ACCESSION   DAOMXO010000714 DAOMXO010000000
VERSION     DAOMXO010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66129_length_1237_cov_5.046531"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLH57_11785"
     CDS             complement(<1..216)
                     /locus_tag="PLH57_11785"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HRK03336.1"
                     /translation="MKRVAILGSILLFATYAEANVSLKNGNFFIGNVDLVYHGGFEPK
                     IQTTYNSKSSHNGIFGFGMGSEFEPYLV"
     gene            complement(251..1138)
                     /locus_tag="PLH57_11790"
     CDS             complement(251..1138)
                     /locus_tag="PLH57_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRK03337.1"
                     /translation="MIATASDTGWRLFYSLLFGAAIFVVAHYYMNRFLDWVRFQSIGT
                     RDYIVEKLGLMFIDFPANRVVLALVLSAVVPFFLVFLLFLPKLIPALIFGLAAGFAGW
                     SAPKFIIGYLFEERILKFNLQMVDGLALMSNGLKSGLSVVQALDMVAEQMPNPMSQEF
                     KLVLSENKVGVSIEEAFNNLAKRVKCEDVEMFVTSINILKETGGNLSETLDTIVYVIR
                     ERVKLEGKVRAMTRQGFYQGMVLLCVPPFIGLYFSTTDPELMGILVEHPIGWVILAAV
                     ITLELAAYFTIKKVVKVDV"
BASE COUNT          371 a          308 c          252 g          306 t
ORIGIN      
        1 caccaaataa ggctcgaatt cgctccccat gccaaaacca aaaatcccgt tgtgggagga
       61 cttgctgttg taggtggttt ggatcttggg ttcgaatcca ccgtggtaaa ccaagtcaac
      121 gtttccgatg aagaaatttc cgttcttcaa acttacgttt gcctcggcgt acgtagcgaa
      181 caataaaatt gatccaagta tagcaacccg tttcatgggc agttccccct cttaaactct
      241 cttccctcta ctatacgtca actttgacga ctttcttgat ggtaaaatag gcggctagct
      301 cgagagtaat cactgcggca agaattaccc agccaattgg atgttctacc agaatgccca
      361 ttaactctgg gtcggtggtc gaaaaataga gcccaataaa tggcggcacg caaagcaaaa
      421 ccatgccttg atagaacccc tgacgagtca tggcccgaac tttaccctcg agttttacgc
      481 gctcgcgtat gacgtaaaca atagtatcaa gcgtttcaga aagattaccg ccggtttctt
      541 tcaaaatgtt aattgaagtg acaaacattt cgacgtcttc gcattttacg cgcttggcta
      601 aattgttaaa tgcctcttca atggaaacgc cgactttatt ttcggaaagc accagtttaa
      661 actcttgtga catcggattc ggcatttgtt ctgctaccat gtcgagcgct tgaacaacgc
      721 ttagcccgga ttttagtccg ttcgacatca gcgccagtcc atcaaccatt tgcagattaa
      781 attttaagat acgctcttcg aacaaatacc caatgataaa cttgggggcg ctccacccgg
      841 cgaatcccgc ggccaaacca aatattaggg caggaattaa cttcggtaag aaaagtaaaa
      901 aaaccaaaaa aaacggaact actgctgaaa gaacgagtgc caacacaact cgattggcag
      961 gaaaatcgat aaacatgagc cctagttttt caacgatata gtcgcgggta ccgatgctct
     1021 gaaaccgcac ccaatccaaa aaccgattca tgtagtaatg ggcaaccacg aaaattgcgg
     1081 caccaaaaag taacgaataa aagagccgcc aacccgtgtc actcgctgtg gcaatcatct
     1141 tacttcaccc cggaaaacaa ggagcgtgga atttttaggc ccttgctttc catttcctct
     1201 acgaacttag gaacaagacc ggtcgaaaca tattttc
//
LOCUS       DAOMXO010000715         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_66130_length_1237_cov_4.927242, whole genome shotgun sequence.
ACCESSION   DAOMXO010000715 DAOMXO010000000
VERSION     DAOMXO010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66130_length_1237_cov_4.927242"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLH57_11795"
     CDS             complement(<1..357)
                     /locus_tag="PLH57_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03338.1"
                     /translation="MIIKNEVQAALGAASTAPGANVYSKAEPGGFGQFLKAANAAPTF
                     SPLEATQNGLGLSMPSISGTMPEVVGTPEMKKEVPVHLTEGPKTKLPKTAIEKTELPK
                     QPMNLDGQTPSLTEGLK"
     gene            complement(359..1054)
                     /locus_tag="PLH57_11800"
     CDS             complement(359..1054)
                     /locus_tag="PLH57_11800"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03339.1"
                     /translation="MTKARLGVRILGLAILLMALLQILRPNTSDASGGGEEQQLTVLD
                     DNTGRVAQELEKRRKELDERERLLKEREEQLSAQGAEIEKRIQRIEEIRAEINGDLKK
                     KRDQSEERITRMIAVFETMAPKSAAQVMETLDDDLSVEVLGRMDPKRVAKIMNIMDKS
                     RSARLSEQLTGYLKPDYENSNKSGRTVASQSSSVTATEQSAGQSRSSSKDGGGAPSGD
                     AGKAVKSEKGGSR"
     gene            complement(1051..>1237)
                     /locus_tag="PLH57_11805"
     CDS             complement(1051..>1237)
                     /locus_tag="PLH57_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HRK03340.1"
                     /translation="LTNKKRQEYVAARQKREALEKLKTKRHQNYLEDVKAHEQRMLDD
                     LYIMRASFLNREKRDAE"
BASE COUNT          237 a          343 c          255 g          402 t
ORIGIN      
        1 ctttaaaccc tcggtcaaac tcggtgtctg accgtccaga ttcattggct gtttcggaag
       61 ttcggttttt tcaattgcgg ttttcggtag tttcgtcttg ggcccctccg tcaaatgaac
      121 aggaacttct ttcttcattt ccggagtacc gacaacttct ggcatggtcc cactaatact
      181 tggcatcgag agccccagtc cattttgagt tgcctctaat gggctgaagg tcggtgccgc
      241 attcgcagcc ttgagaaatt gtccgaagcc acctggttcc gcttttgaat acacatttgc
      301 gccaggggca gtactcgctg ccccaagcgc ggcttgaact tcatttttta tgatcatttt
      361 atctgctacc tcctttctca gatttaactg ccttgccagc gtcgccactc ggcgctccgc
      421 ccccgtcttt cgacgaagat cggctttgac ccgctgattg ttcggtggca gtaacgctac
      481 tactctggct cgctactgtc cgacctgatt tgtttgagtt ttcgtaatct ggcttcagat
      541 atccggtgag ttgttccgaa agtctcgcag accgggactt gtccatgata ttcataattt
      601 tcgctacacg cttcgggtcc attcttccca acacttcaac cgacaagtca tcgtccaacg
      661 tttccatcac ttgggcagcg gacttcggag ccatagtttc aaacactgcg atcatgcgcg
      721 taatacgctc ttcactctga tctcgctttt tctttaaatc accgttaatc tcagcgcgta
      781 tttcttcgat ccgttgaata cgcttttcga tctctgcacc ttgagcgctc aattgttctt
      841 cacgttcttt aagcagccgt tcacgctcgt ccaattcttt tcgccgtttc tcgagctcct
      901 gcgctactcg ccctgtgtta tcatctaaaa ccgttagctg ttgttcctct ccaccgccag
      961 atgcatcgga agtattcggc cgcagaattt gaagtaaggc catcagtaaa attgctagac
     1021 ctagtattct tacaccgagt cgcgctttcg tcattccgca tctcttttct cgcgattcag
     1081 aaaactcgct cgcatgatat acaagtcatc taacattcgt tgctcgtgag ccttaacgtc
     1141 ttctaggtag ttttgatgac gtttggtttt caatttttcg agtgcttcac gtttctggcg
     1201 agccgctaca tattcctgcc gttttttgtt tgttaac
//
LOCUS       DAOMXO010000716         1227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_66844_length_1227_cov_6.180034, whole genome shotgun sequence.
ACCESSION   DAOMXO010000716 DAOMXO010000000
VERSION     DAOMXO010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66844_length_1227_cov_6.180034"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLH57_11810"
     CDS             complement(<1..572)
                     /locus_tag="PLH57_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03341.1"
                     /translation="MKLRENVLKWSILTGLVIALFASNAESAVISRQIVGEGGEEPFY
                     AKFADTKAELKDPEVRKQVGIYVLNQSGELEKVVEGQILFHRIKGPILHRFETTVYNG
                     LHAMAAIVDNRLKIFSLASRLKDPESVIELGSKGAQLLDSATNRVVTLPLIQDAENVN
                     FITGEATKKGQTFVVSIKFPKKDARGNGVT"
     gene            complement(595..>1227)
                     /locus_tag="PLH57_11815"
     CDS             complement(595..>1227)
                     /locus_tag="PLH57_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03342.1"
                     /translation="TLTVAVAREAFEQLLNREEDIVVPDNNARLTWLGYADRVIVDLI
                     VPEMRRDIMRAWGAEEGNARRIYDMLLKELMNSSGRGPSERLKDVMKFYLEVNREPLV
                     PANITEFLARAQERLLETGGKGFVDEPHPGLMEAIHEYLADQQSKIVPLTVIADSIAG
                     IETDAASSEVREAFKKVMIHELGYASDRAIADAIATVDEVLSKKEELESR"
BASE COUNT          311 a          327 c          255 g          334 t
ORIGIN      
        1 aacgttactc cgtttccacg agcatctttt ttcggaaact taattgaaac aacaaaggtt
       61 tggccttttt tcgtagcttc accagttatg aagttaacat tttcagcgtc ttgaataaga
      121 ggaagcgtta ctacccggtt ggtagctgaa tccaacaact gagctccctt tgatccaagc
      181 tcgatcacgc tttcagggtc ttttagtcgg ctcgcgagcg aaaaaatctt aaggcggtta
      241 tctacgattg ccgccattgc atgcagaccg ttgtagactg tcgtttcgaa tcgatggaga
      301 ataggtcctt taattctatg aaaaagaatt tgaccttcga caaccttttc cagttcgccg
      361 ctctggttca aaacgtagat tccaacttgt tttcgaactt ctgggtcttt tagctctgcc
      421 ttcgtgtccg caaacttggc atagaaaggc tcttcaccgc cttcaccaac gatttgccga
      481 ctgataaccg cgctttcagc gttcgacgca aaaagcgcaa tcaccagacc agttagaatc
      541 gaccatttca gaacgttctc tcggagcttc acaacaaaca gccctcttta atgattagcg
      601 agactcaagt tcttcttttt tggagagcac ttcatccaca gttgcgattg catcagcaat
      661 tgcgcgatcg gaagcatacc ccaactcatg gatcatcact tttttgaagg cttcccgcac
      721 ttcggaactc gcagcatcag tttcaattcc agcaatcgaa tcagcgatta cagttagcgg
      781 aacaattttc gactgttgat cggcgagata ctcgtgaatt gcttccatga gtcctgggtg
      841 cggttcatcg acaaaacctt tgcccccagt ttccaaaagt cgttcctgag cgcgcgccaa
      901 aaactcggta atatttgcgg gcaccaacgg ctcacgattc acttcgagat agaatttcat
      961 tacgtcttta aggcgctccg acgggcctcg cccactagaa ttcatgagct ctttaagaag
     1021 catgtcgtaa atgcgccggg cgttaccttc ttcggccccc caagcgcgca taatgtcacg
     1081 tcgcatttcg ggtacaatca aatcaacaat gacccggtca gcgtaaccca accaagtgag
     1141 ccttgcattg ttatcgggaa caacaatgtc ttcctcgcga ttgagcaact gctcgaacgc
     1201 ctctctagcg accgccacag ttaaagt
//
LOCUS       DAOMXO010000717         1227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_66856_length_1227_cov_4.702218, whole genome shotgun sequence.
ACCESSION   DAOMXO010000717 DAOMXO010000000
VERSION     DAOMXO010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66856_length_1227_cov_4.702218"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1227
                     /locus_tag="PLH57_11820"
     CDS             <1..>1227
                     /locus_tag="PLH57_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRK03343.1"
                     /translation="GRKIWLCFEKAVPRSEVEAYASKVFFNELIETCEILGPDDSENI
                     QRKFSDSQLAVHFRLPSSTEVDVETIDVANLTVEEFVELSRKRLWAFSQDECETLIRH
                     YRKLARAATDVEIEVFAQTWSEHCKHKIFAADIAVTNERDDLALPERVAGVFKTYIRG
                     STEKITKPYLRSVFHDNAGVIAIGNDHERVDVAIKVETHNSPSALDPYGGALTGIVGV
                     NRDILGVGLGAEPIANLDVFCVGSLEHREELPERLHHPRRILEGVRSGVQDGGNKSGV
                     PTVTGALVHDRSYLGKPLVFCGTIGVIPYSDQKDDLLTKEITSGDAIVMVGGRIGKDG
                     IHGATFSSLELNQDSPASAVQLGDPLTQRRVWDFLLEARDARLYSAVTDNGAGGLSSS
                     IGELARLCGQRGGAKL"
BASE COUNT          306 a          271 c          347 g          303 t
ORIGIN      
        1 ctggacgaaa aatatggctg tgttttgaaa aagcggtacc gcgctcggaa gtcgaagctt
       61 acgcttctaa agtatttttt aacgagctta ttgagacttg tgaaatactt ggaccagacg
      121 attcggaaaa tattcaacgc aagtttagcg attcgcaact tgcggtgcat ttccgcttgc
      181 caagttctac cgaagtagat gtagaaacga tcgatgtagc caatctcacg gtcgaagagt
      241 tcgtggaact tagcagaaag cggctttggg cgttttccca agacgaatgt gaaaccctga
      301 ttcggcatta ccgaaaactt gctcgagcag cgactgatgt cgaaatagaa gtgttcgcgc
      361 aaacatggtc ggaacactgt aaacacaaaa tttttgcggc agatatagct gtaaccaatg
      421 agcgcgatga tttagctctt cccgagcgtg tggctggagt ttttaagacc tacattcgtg
      481 gatcaaccga aaaaattacg aaaccttatt tacgaagcgt atttcacgac aacgccggtg
      541 tgattgcgat tgggaacgat catgagagag tcgacgtcgc cattaaggtt gagacccaca
      601 attccccatc tgcgctggac ccctatggtg gcgcgctgac aggcattgtt ggggtgaatc
      661 gcgatatttt gggtgtgggg ctcggtgcgg agccgatcgc gaatctcgat gtattttgcg
      721 tgggttccct cgagcatcgg gaagagctgc cggagcgcct tcatcacccg cgtcgaattt
      781 tggaaggcgt gcgatcggga gtacaagacg gaggcaacaa aagtggtgtt ccgactgtca
      841 ctggcgcact ggttcacgac cggagttatc tcggtaagcc tttagtgttt tgtggcacga
      901 tcggcgtgat tccctattcc gatcaaaaag atgatttgct aacaaaagaa attaccagtg
      961 gcgatgcgat tgtgatggtc ggcgggcgaa ttggaaaaga cggtatccat ggcgccactt
     1021 ttagttcgct tgaactaaat caagattcac ctgcaagcgc tgtgcaacta ggtgacccgc
     1081 tcacccagcg tcgagtttgg gatttcctgc tcgaggcaag agatgcgcgg ttgtattccg
     1141 cggtgaccga taacggcgct ggcggcttgt cttcaagcat tggagaactt gccaggttgt
     1201 gcggccaacg tgggggagcg aagctag
//
LOCUS       DAOMXO010000718         1225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_67000_length_1225_cov_4.570085, whole genome shotgun sequence.
ACCESSION   DAOMXO010000718 DAOMXO010000000
VERSION     DAOMXO010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67000_length_1225_cov_4.570085"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLH57_11825"
     CDS             <1..1209
                     /locus_tag="PLH57_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03344.1"
                     /translation="VVEEEVRVPLENDDKIDLDPNDARDSSSRVMIGQSIAALDSIPM
                     PRIAREYSIKSELDQELCIEHVFNPEWKRQRTARSQGYDPERKEREVQKERTELLEQL
                     CGFREENYRAEDNVPTFGDLLRSREPKVVYTRNSAIDGYGAGIGATVLQSSGGAAGFR
                     GSVHIDANPKGANRVLLDTETGITMQTEFVIDPTNDQTKIPYARLTVKGTNGLDKVGS
                     GDSFAADGARSRLAAKAPTERVHEYTIERNADLDRFDAHVKWGRRGRKNLTNGSYESG
                     TLGWYLPWVWTAKAGVGTIDGQLNGHIGAEAGLEERYCKTLKKNHRACVSSALLGTLG
                     YLEAGLELRLGGEYEYTIESERGDTDKLLNETVFSAGIENTYNYVYDAHGEHDSNRAF
                     VGVAVEYRTE"
BASE COUNT          352 a          294 c          328 g          251 t
ORIGIN      
        1 gtcgttgaag aggaagtgag agttccttta gaaaatgacg acaaaattga tttagatcca
       61 aacgatgcac gagattcgag ctctcgggtt atgatcgggc aatctatcgc tgctctcgat
      121 tcgattccta tgccgcgaat tgcccgtgaa tattctataa aaagtgaact cgaccaggag
      181 ctatgcatcg aacacgtttt taacccagag tggaaacggc agcgaaccgc tcgcagccaa
      241 ggctacgatc ccgaacgcaa ggaacgtgaa gttcaaaaag aacgcaccga actattggag
      301 caactatgtg gttttcgcga ggaaaattac cgagctgaag acaacgtacc cacttttggt
      361 gatttactgc ggtcgcggga acctaaagta gtttatactc gcaacagcgc gatcgacggc
      421 tacggcgccg gtatcggtgc gaccgtactt caaagtagtg gaggagcagc gggatttcga
      481 ggttcagtgc acatcgatgc aaatcccaaa ggagccaatc gcgttctact cgacactgaa
      541 actggaatta cgatgcagac agaattcgtg attgatccaa caaacgatca aaccaaaatt
      601 ccgtatgcac gcctaaccgt aaaaggcact aacggtttgg ataaagtcgg ttcgggcgat
      661 tcgttcgcag ccgatggggc tcgcagtcgc ctagcggcaa aagctccaac tgaacgagtt
      721 cacgaatata ctatcgaacg caacgccgat cttgatcgct tcgacgccca cgtaaaatgg
      781 ggacgccgcg gtcgcaaaaa tctcacgaat ggtagctatg aaagcggcac cttgggctgg
      841 tacctacctt gggtttggac tgcaaaagca ggcgtgggca ctatcgatgg ccaattaaat
      901 gggcacatcg gagcagaagc cgggctagag gaacgttatt gcaagacact taagaaaaac
      961 caccgggcct gcgtgtcgag cgcgcttctg ggcactcttg gttacctaga ggccggcctc
     1021 gaactgcgtt tgggtggtga atacgagtac accatagaaa gcgaacgggg cgataccgac
     1081 aaactgctca acgaaaccgt attttcagca ggaatcgaaa acacctacaa ctacgtttac
     1141 gatgcccacg gagaacacga cagcaatcgc gctttcgtgg gtgtggcagt agagtatcga
     1201 accgaatagt gacgaaagtg tgagg
//
LOCUS       DAOMXO010000719         1225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_67017_length_1225_cov_3.858974, whole genome shotgun sequence.
ACCESSION   DAOMXO010000719 DAOMXO010000000
VERSION     DAOMXO010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67017_length_1225_cov_3.858974"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            680..>1225
                     /locus_tag="PLH57_11830"
     CDS             680..>1225
                     /locus_tag="PLH57_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HRK03345.1"
                     /translation="MSASMDLFDKCRSYQDAKALINTGLYPFFRPIEASTGNTVVTHG
                     TKRVMIGSNNYLGLTHHPKVKEAAKRAIDEYGTGCTGSRFLNGNLTSHEKLEKSLAEF
                     VGKEAALVFATGFLANQGSLSCLIGKNDAIFSDRENHASIIEGTRLAMGETVRYPHND
                     IDALDKMLTDKRSNYDGVLIVA"
BASE COUNT          311 a          264 c          306 g          344 t
ORIGIN      
        1 ctgataactc gaaacccctt tgcggcttcg aattttgttc gacgcttttt tgcaagcggt
       61 cagtaagtaa accactagat cgagccactg cgctggagtc ttatcgagtg gcgctgtggg
      121 gtctgtgtcc gtttcttctg atgaaggtaa gtttttggac acctttggga ctaattcttt
      181 tggggggagg gggttgttat tcggttgaac caatccaggt gtgggagccg cgatcttgct
      241 ttctagtaaa ttgccgctgg ccgttttgga atggtgtctc ggctttggat cgccaggaga
      301 gcggggaaaa cccactcgaa tgggtcgcaa aaaacgcaaa agcgacttcg cgagccgatt
      361 gctcagcaaa ttggcggcat tctgattgtt gttttccacg gtaaaaacac ctcgtcttcg
      421 tcttgcgcga tttcgaagtc ttagccgtgt gaagtgtact tcctagccct gcgtaaaatg
      481 ctacacaaaa cacactaatt gcattaagct tatcggtaag cttttcgaaa tactttagta
      541 aaaatattgt aagaattaga cttaataatt gtccattatt atcagttatt taggagtttg
      601 tttgacgagg ctgcggtgac ggtgctatcc acctggcctt tcatacctaa gttaacgaca
      661 acgcgaagga gcaatcagca tgtcggcttc catggatctt ttcgataagt gccgcagtta
      721 tcaagatgca aaggctttaa taaacactgg tctttacccg tttttcagac cgatcgaagc
      781 tagcactgga aacactgtcg ttacccacgg cacaaagagg gtcatgattg gctccaacaa
      841 ttacctgggt ctgacccacc atcctaaagt gaaagaagcg gccaagcgcg ccatcgacga
      901 atacggtacg ggttgtactg ggtcacgatt tttgaacggc aatctcactt cccacgagaa
      961 gctcgaaaaa agtttagcgg aatttgtagg taaagaagct gctctggtgt ttgcgactgg
     1021 ttttttggca aatcagggtt cactttcttg tctaatcggt aaaaacgacg cgattttttc
     1081 agaccgagaa aatcatgcat caatcattga agggactcgt ctggcgatgg gcgagactgt
     1141 tcgttacccg cacaatgata tcgatgcgct cgataaaatg ctgacagata agcgtagcaa
     1201 ctacgatggt gttttgatcg tggct
//
LOCUS       DAOMXO010000720         1222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_67212_length_1222_cov_5.119109, whole genome shotgun sequence.
ACCESSION   DAOMXO010000720 DAOMXO010000000
VERSION     DAOMXO010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67212_length_1222_cov_5.119109"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..798
                     /locus_tag="PLH57_11835"
     CDS             178..798
                     /locus_tag="PLH57_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03346.1"
                     /translation="MRCCCWQKFKLFNIALICLALSVANSLARPALADWVAVDAGVFL
                     PRPAGVALDRNGSKSFYEPRQYYIGPRVGLPLGEAQRVEFATWFLLPWDSNLDGGSQT
                     QVATLFRAALGHFVSKRVLLSGGTTFRWVYISSTAQSVTQRNGSTTRTFFTPSQNRST
                     WSASIDASVGVYLIKDLRLSFDVLVWRALSEYRSASVAAGAHYAFF"
     gene            785..>1222
                     /locus_tag="PLH57_11840"
     CDS             785..>1222
                     /locus_tag="PLH57_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03347.1"
                     /translation="MRFFKTNGLRAFGAIILMSLYSSNSAHAWTLLYESAFKVGGSTM
                     SVTVNRTNCPSGIDGWLEKAAELWSAIDTSYLEIKIEGDSSATASNIYNYELLGFSVV
                     CDTNFGTTTGADPNSAIAVGGSWIDESGNLAKGGVIINLQGTVF"
BASE COUNT          282 a          268 c          344 g          328 t
ORIGIN      
        1 actaggaaaa aatacgcgca tgcagaatgc atacgccacg gtcgcacttt cttgtgcatt
       61 gcttttaccc ccttgtaaaa accacgacga attttgaata cttctaagga gctatcacgg
      121 aagaaaaagc ttgtaaaaga ttttgccgat cgctaccaaa aaagatcggg ggcatgattg
      181 cgttgctgtt gctggcaaaa attcaagctg tttaatatcg cgctaatttg tttggcgctt
      241 agtgtcgcaa atagtcttgc gcgtcccgcg ctggctgatt gggtggcagt cgacgccggg
      301 gtgtttctcc ctcggccagc tggtgttgcg cttgatcgca acggttcgaa gtcgttttac
      361 gaaccacgac aatattatat tgggccgcgc gtggggctgc cactcggcga agcgcagcga
      421 gtggagttcg cgacctggtt cttattgcct tgggatagca atctagacgg cggatctcaa
      481 actcaagtgg ctacgctgtt tcgagctgcg cttggtcatt tcgtgagcaa gagagttttg
      541 ttgagcggag gtactacatt tcgatgggta tacatttcgt ccactgcgca atcggtgacg
      601 caacgcaacg gttcgacaac caggactttc tttacaccgt cgcagaatcg cagcacctgg
      661 agcgcgtcca ttgacgcgag tgttggagtg tacttaataa aggatttgcg attgtccttt
      721 gatgttttgg tgtggcgggc cctatcggag tatcgtagcg cgagtgtcgc tgcaggagcg
      781 cattatgcgt ttttttaaaa cgaacggtct tcgggcgttt ggcgcgatta tattaatgtc
      841 gctatatagt tccaacagcg cccatgcttg gacattgttg tatgagtcgg catttaaggt
      901 gggcggtagt accatgtcgg tcactgtcaa tcggacaaac tgcccgtcag gcattgatgg
      961 ttggctagaa aaggccgccg aattatggag tgcaatcgat acgtcttatc tcgaaataaa
     1021 aatagaggga gactcgagcg ccacggcctc gaacatttac aattacgaac ttctagggtt
     1081 ttcggtggtg tgtgatacga attttggaac caccacgggc gcagatccaa attctgctat
     1141 cgcggtaggg ggatcgtgga ttgacgagtc cggaaatctc gcaaagggcg gggtgattat
     1201 taacttgcag ggcacggttt tt
//
LOCUS       DAOMXO010000721         1219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_67448_length_1219_cov_4.884880, whole genome shotgun sequence.
ACCESSION   DAOMXO010000721 DAOMXO010000000
VERSION     DAOMXO010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67448_length_1219_cov_4.884880"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..>1219)
                     /locus_tag="PLH57_11845"
     CDS             complement(23..>1219)
                     /locus_tag="PLH57_11845"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRK03348.1"
                     /translation="VGKISEKDYGAQVQRAIRLLEGRGSQVLDELHTMMAEASEREDY
                     ERAAVLRDRIRALEAVNEQQKVTDPESNANRDIVHMARGESADGKASRAVVVVLNVRE
                     GRAVGVFQYPFENVDPDMASPEVLFEFLSQYYLSRSETSSQLLPREVLLPEECEVLMK
                     DQIRVLESALGRAIEFRIPRRGEALELVSMVKKTADHRLTDIASRVGSAMEDLNDVHY
                     RLALERFPSRIECYDISHFQGEGTVASRVVFLDGRPEKDLYRRYHVREVTGVDDFGSL
                     REVLGRRFENDQSLPDLVVIDGGKGQLSQAEAIFKELGVVGVDLVSIAKARTERDFGA
                     SEVKASFERIFKPGQKNPIMLKPATGAFRVLTQVRDEAHRFAITFHRKVRAKKRVSKR
                     KSNNKD"
BASE COUNT          275 a          342 c          293 g          309 t
ORIGIN      
        1 ctttggtcgc agtgccgagt aattaatctt tattattcga ttttcttttt gaaactcgtt
       61 ttttggcccg cacttttcga tgaaaggtga tcgcgaatcg atgggcttca tcacgcactt
      121 gagtaagcac gcggaaggcg ccagtcgctg gttttaacat aatcggattt ttctggcccg
      181 gtttaaaaat tcgctcgaat gaagctttca cttcggaagc tccgaaatcg cgctcagtgc
      241 gcgccttggc gatggaaact aagtctacgc caaccactcc gagttcctta aaaatcgctt
      301 cggcttgaga aagctgccct ttgccgccgt caatgaccac gaggtctggc aaagactggt
      361 cgttttcaaa cctgcgacct agtacttcgc gaagactgcc gaagtcgtcg acaccggtta
      421 cttcacgtac gtgataccgc cgataaaggt ctttttcagg gcgaccgtct aaaaacacca
      481 ctcgagatgc aaccgtgcct tccccttgaa aatgcgaaat gtcataacac tcgatgcgac
      541 tcggaaagcg ctcgagtgct aaacgatagt gcacatcgtt taggtcctcc attgcacttc
      601 ccacgcggct tgcgatgtcg gtaagtcgat ggtcggcggt ttttttgacc atcgaaacca
      661 actcaagcgc ttcgcctcgc cggggaatac gaaactcaat ggcgcgccca agagcgctct
      721 cgagcactcg aatttggtct ttcattaata cttcacattc ttcaggcaaa aggacttccc
      781 gagggagcag ttgggaactc gtttcagagc gcgacaaata gtactgtgac aaaaattcaa
      841 atagcacttc cggcgaagcc atatcggggt cgacgttctc aaacgggtat tgaaacacac
      901 ctaccgcgcg cccttcccgc acgttgagca ccaccactac cgcacgcgag gcctttccgt
      961 cggccgactc accgcgcgcc atgtggacta tatctcgatt ggcgttgctt tcggggtccg
     1021 ttactttttg ctgctcattc accgcttcga gtgctcgaat gcgatcgcgc aacaccgcgg
     1081 cacgttcgta atcttcgcgc tcagaagctt cggccatcat ggtgtgtaac tcgtctaaaa
     1141 cttgggagcc tcgcccttcg agcaggcgaa ttgcgcgctg tacttgggcg ccgtagtcct
     1201 tttcgctaat tttaccaac
//
LOCUS       DAOMXO010000722         1217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_67600_length_1217_cov_6.777969, whole genome shotgun sequence.
ACCESSION   DAOMXO010000722 DAOMXO010000000
VERSION     DAOMXO010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67600_length_1217_cov_6.777969"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLH57_11850"
     CDS             complement(<1..385)
                     /locus_tag="PLH57_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRK03349.1"
                     /translation="MRAAQIRTEFLKYFEKNGHTVVESSGLVPHNDPTLLFTNAGMVQ
                     FKDVFLGSDPRPYKRAATSQKCVRAGGKHNDLENVGFTARHHTFFEMLGNFSFGDYFK
                     KDAIHFGWEFITKNLGIPKDRLYVSV"
     gene            477..>1217
                     /locus_tag="PLH57_11855"
     CDS             477..>1217
                     /locus_tag="PLH57_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03350.1"
                     /translation="MLLKNLRRHSALLGAGIVALVAAGCPTPKETANAVSKLIAREGA
                     SFEETRGLNAPSGTYIHGEHIAQSLGAWAANAHEVVRELNSLADKLRDSGTKAEDKVS
                     PQTLNAIKAQLQEMLVRVVDIRLTVPEWQLNSAQSSLKALVAAYKALPTAYAGVFEEN
                     ITFTGSIKVAIELDPEMYDTKKDCETELRIYAAETQEPFCRAHAYSVCGHQMDLKCLS
                     DLLNSAARTQKKCEHITTDDQGREKKTGM"
BASE COUNT          327 a          276 c          316 g          298 t
ORIGIN      
        1 atacgctcac gtaaagacga tctttaggaa ttcctaaatt ttttgtgatg aactcccagc
       61 caaagtgaat ggcgtctttt ttgaagtagt cgccaaacga aaaattaccg agcatttcaa
      121 aaaaagtatg atggcgcgcc gtgaagccta cattttcgag atcattgtgt ttgccgccag
      181 cacgcacgca tttctgagag gtcgctgccc gtttgtaggg ccgagggtcg gaacccagaa
      241 aaacgtcctt gaattgcacc atgccggcat tggtaaaaag aagagtgggg tcgttgtggg
      301 gaactaagcc cgacgattcg accactgtgt gaccattctt ctcaaaatat ttcaaaaatt
      361 cggttcgaat ttgcgctgcg cgcatagtga ggctcctgcg ccaagacgtt atccgatttc
      421 gttcgtttga cgaaggtttt ttttactgct acccggtgtc tacaaggggg atctatatgt
      481 tattgaaaaa tttaagacgt cattccgcgc ttttgggggc cggaattgtg gcattggtgg
      541 ccgcaggctg cccaacgccc aaagaaactg ccaacgccgt aagcaagttg attgcgcgtg
      601 aaggggcatc tttcgaagaa acaagaggcc ttaatgcgcc gagtggcacc tatattcatg
      661 gcgagcacat cgctcagtct ttaggggctt gggctgcgaa tgcgcatgaa gttgtacggg
      721 agctaaacag tttagccgat aaattgagag actccggaac taaggctgaa gacaaggtgt
      781 cgcctcaaac gctcaatgcc atcaaagcac agctgcaaga aatgcttgtg cgtgtggtcg
      841 atattcgttt gaccgttcca gagtggcagc tcaattcggc gcaaagttcg ttaaaagcgc
      901 tagtcgcagc ctataaagcc ttacccacgg cgtacgctgg cgtgtttgag gagaacataa
      961 cctttaccgg ttcaatcaag gtggctatcg aacttgatcc cgaaatgtac gacaccaaaa
     1021 aagattgcga aaccgagttg cgaatttatg ccgctgaaac gcaggaaccg ttttgccggg
     1081 ctcacgcgta ttcggtgtgt ggccatcaaa tggaccttaa atgtttgagt gatcttttga
     1141 attccgccgc tcgcactcaa aagaaatgtg agcacataac caccgatgat caaggtcgag
     1201 aaaagaaaac gggcatg
//
LOCUS       DAOMXO010000723         1216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_67724_length_1216_cov_3.290267, whole genome shotgun sequence.
ACCESSION   DAOMXO010000723 DAOMXO010000000
VERSION     DAOMXO010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67724_length_1216_cov_3.290267"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PLH57_11860"
     CDS             complement(<1..171)
                     /locus_tag="PLH57_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRK03351.1"
                     /translation="MGLATLCSRVFGLVREQVFAFYFGATDAADAFNIAFRIPNLLRD
                     LFAEGAMSAAFVP"
     gene            complement(197..469)
                     /locus_tag="PLH57_11865"
     CDS             complement(197..469)
                     /locus_tag="PLH57_11865"
                     /inference="COORDINATES: protein motif:HMM:NF021188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HRK03352.1"
                     /translation="MEYAANGSAAEIYLKLKKKLEDYRNAGKLEQLKEVTFDDAAHTA
                     RATGPGFKANIACKDGKVVVELDLNFLLKPMRGKIEEKIGRMVASV"
     gene            572..>1216
                     /locus_tag="PLH57_11870"
     CDS             572..>1216
                     /locus_tag="PLH57_11870"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRK03353.1"
                     /translation="MKTSFTDLIGSKYPIIAGPMFLVSDETLVTAACDAGIVGACPSL
                     NWRTTEQFRQALKTIKQSVGTKPFAVNIIVNKSNLRAEPDLKACLDEGVPLFVTSLGS
                     PKQVIADAHKNGAKVFCDVVNVEYAKKVQDLGADGVIAVSQGAGGHAGPISSMVLVPY
                     LKKHLSIPIVAAGGIATGRGLAAALALGASAVQIGTRFIASTECRVNESYKKAIV"
BASE COUNT          298 a          319 c          286 g          313 t
ORIGIN      
        1 aggcacgaaa gccgcactca tggccccttc ggcaaagaga tctcgcagta aatttggaat
       61 tcgaaaagct atattaaacg cgtcggccgc gtcggtggca ccaaaataaa aggcaaagac
      121 ctgctcacgc actagcccaa aaacgcgact gcacagcgtg gcaaggccca tttttccggc
      181 agacttggca acagaactag acactcgcga ccattcttcc gatcttttct tctatttttc
      241 cacgcattgg cttaagtaaa aaattcaaat ccaactccac caccactttg ccatctttgc
      301 aggcgatatt agccttaaat ccgggtccgg tcgcccttgc ggtatgagcc gcgtcgtcaa
      361 acgtcacctc tttgagttgt tcaagtttac cggcgtttcg gtagtcctcg agcttttttt
      421 taagttttaa gtaaatctct gccgcactgc cgtttgctgc gtattccata gtgagctcct
      481 ctgttcccag ggtggcaact ctgatttgcc acttcactaa tcgattttac tagggcatgt
      541 tttcgttgtc ataaatttat aggcaaattc catgaaaact tcgttcactg acctcattgg
      601 ttcaaaatat ccgatcatcg caggcccaat gtttttggtc agtgatgaaa cgctcgttac
      661 cgcagcttgc gacgcaggta tcgtaggggc gtgtccgtcg ctcaactggc gcacgacgga
      721 acagtttcgt caggccttga aaaccatcaa acagagtgtg ggaactaaac cgtttgctgt
      781 gaatattatt gtaaacaaaa gtaatctccg ggctgagccc gatcttaaag cctgccttga
      841 tgaaggggtt cctctcttcg taaccagtct tggcagccct aaacaagtca tcgccgatgc
      901 ccacaaaaac ggtgcgaagg ttttttgtga cgttgttaat gtagagtacg ccaaaaaagt
      961 tcaagaccta ggggccgacg gcgtcattgc ggtctcccaa ggtgccggtg gccacgctgg
     1021 cccgattagc agcatggtcc tcgtaccgta tttaaagaaa cacttgagca tcccaattgt
     1081 tgcggccggg ggaattgcta ccggtcgcgg tctcgccgcg gcgctcgccc tgggcgccag
     1141 tgccgtacaa ataggtacca gatttattgc atcgaccgag tgccgcgtaa acgaatccta
     1201 taaaaaagcg attgtt
//
LOCUS       DAOMXO010000724         1215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_67780_length_1215_cov_5.156897, whole genome shotgun sequence.
ACCESSION   DAOMXO010000724 DAOMXO010000000
VERSION     DAOMXO010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67780_length_1215_cov_5.156897"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1215
                     /locus_tag="PLH57_11875"
     CDS             <1..>1215
                     /locus_tag="PLH57_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03354.1"
                     /translation="DLLLFSAELLSLSGSDSSLFWNGLISLWNKNRNSKFFGADLLIT
                     RIEKVAKKNPKLATFLSDSPAAFFSFLESLSDKWIELTKERSNTLDSRRAFFLEEAIR
                     NLTTTISRFAWVAPKLGTSTFGYPAPSVPTAIWYRFHRADFKIDFSKERALLASWIER
                     HWETLDLSFVYSVWVWTLAPDTALVPAIILNFHRLAFESPETFRLFIRLFGPNQPEPV
                     EALKFQTYANPLFVRNLVTNPIYSKMLAIDESFAKKILHFIEQPDFTTHYAGALVAFL
                     FSTAESPRGILWRDGLLSGVSEQGLHNFIRLFQYWNNLDRNKQMEFLNDRFQEYEAEL
                     IGRTARLAFQGPANIDSKVAALKKSGANIPKDLLDHRERLVEIRKILRRFSQEQNPEA
                     KKIISDELLKEI"
BASE COUNT          333 a          288 c          240 g          354 t
ORIGIN      
        1 tgatcttctc ttattctcgg ctgagctttt gagtctatcg gggtccgaca gttcgctttt
       61 ttggaatggt ttgatctcac tctggaataa aaatagaaac agtaaatttt tcggtgctga
      121 ccttttaatt actcgaatcg aaaaggtcgc aaaaaaaaat ccgaagttgg cgacatttct
      181 ttccgactct cctgccgctt ttttttcatt tttggaatca ctctccgata agtggatcga
      241 actaaccaag gaacgaagta acaccttaga ctcacgcaga gcttttttcc tggaagaggc
      301 tatacgaaac ttaaccacca cgatttcacg tttcgcttgg gtagccccca agctaggaac
      361 ttcgacgttt ggctaccctg cgccctcagt accaaccgct atttggtacc gcttccatcg
      421 cgcagacttt aaaattgatt tttctaagga acgcgctttg ttggcgtctt ggatagaacg
      481 acattgggaa acattagatt tgtcgtttgt ttattcagtt tgggtttgga ccctcgctcc
      541 cgataccgct ctagttccgg ctattatttt aaattttcat cgattggcat ttgaatcccc
      601 tgaaactttc agattattta tacggttatt tggtcctaat cagccagaac ccgtcgaggc
      661 cctgaagttt caaacctacg caaatcctct gttcgtaaga aacctggtta ccaaccccat
      721 ttactcgaag atgctcgcga tagacgagag cttcgcaaaa aaaattctac actttataga
      781 acaacccgac tttacaaccc actatgcagg cgccttagtt gcatttttat tttcgaccgc
      841 cgaatcaccc cgcggcattt tatggcgcga cggcctttta tcgggcgtat cggagcaagg
      901 actccacaat tttattcgac tctttcaata ctggaacaac ttagatcgca ataaacagat
      961 ggaatttctg aatgatcgat ttcaggaata cgaggcagaa ctaattggcc gaacagcgcg
     1021 attggccttc caaggcccgg ccaacattga ttccaaagtt gccgctttaa aaaaatcggg
     1081 cgctaacatt ccgaaagatt tattagacca tcgcgaacgc ctagtagaga ttcgtaaaat
     1141 tttgcgtcga ttttcgcagg aacaaaatcc agaggccaaa aaaataattt ctgacgagtt
     1201 actcaaagaa attca
//
LOCUS       DAOMXO010000725         1214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_67867_length_1214_cov_4.633305, whole genome shotgun sequence.
ACCESSION   DAOMXO010000725 DAOMXO010000000
VERSION     DAOMXO010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67867_length_1214_cov_4.633305"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1214)
                     /locus_tag="PLH57_11880"
     CDS             complement(<1..>1214)
                     /locus_tag="PLH57_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRK03355.1"
                     /translation="ICFVNKMDRAGANPWRGIQQLREKLGHNAHAITMPIGYEDKFQG
                     VIDLVAMKAFYFDGDNGEKIREEAIPAELMDEAKKRREELVAALGDFDDVVAEKYLGG
                     EEVKPEELRPAIRKATLSLKFTPVMMGSAFKNKGVQLLLDAVGYYLPNPGEVYNEALD
                     LDKGEEKVTLKSDPELPFVGLAFKLEDGRYGQLTYMRIYQGTVKKGDFIYNMANRKKV
                     KIPRLVRMHADKMDDIESASAGDIVAMFGVECASGDTFTDDRVHLTMTSMHVPNAVIS
                     LAIAPKEKAGQANFSKALNRFTKEDPTFRVHRDEESAQTIISGMGELHLEIYCERIKR
                     EYNCEVTVGKPQVAFRETISQKGEFEYTHKKQTGGSGQFGKVIGYIEPLPADAVEKFE
                     FVNDIVGGSIPR"
BASE COUNT          274 a          317 c          290 g          333 t
ORIGIN      
        1 cacgtggaat cgagccaccc acgatgtcgt tgacgaactc gaacttctca accgcgtcgg
       61 caggaagggg ttcgatgtaa ccaatgactt taccaaattg acctgaacca ccagtttgtt
      121 tcttgtgagt gtattcgaac tcgccttttt ggctaatcgt ctcacggaag gccacctgtg
      181 gtttgcccac ggtaacttca cagttgtact cgcgcttaat gcgttcgcaa taaatctcta
      241 agtgcaactc gcccataccg ctgatgatcg tttgagcgga ctcttcgtcg cgatgaacgc
      301 gaaacgtagg gtcttctttc gtaaagcggt tgagcgcttt agagaagttc gcctgacctg
      361 ctttttcttt aggggcaatc gcaagtgaga tcaccgcgtt cggtacgtgc atcgaagtca
      421 tcgttaaatg aacacgatcg tccgtgaaag tgtcgccgga ggcgcactca acgccgaaca
      481 tagcaacaat gtcgccagcc gaggccgatt cgatatcatc catcttgtcc gcgtgcatgc
      541 gcacgagtcg cggaattttg accttcttgc ggttggccat attgtaaatg aagtcaccct
      601 ttttgacggt gccttggtaa atacgcatgt aggtcaattg tccatagcgg ccgtcttcga
      661 gcttgaacgc aagtccgacg aacggaagct ccggatcact tttgagcgtg actttttctt
      721 cgcccttatc gagatcgagt gcttcgttgt acacttcgcc tggatttggc aaataataac
      781 cgaccgcatc aagcaatagt tgaacacctt tgtttttaaa ggccgagccc atcatgacag
      841 gcgtaaattt caacgaaagc gtggctttgc gaatagctgg gcgtagttcc tctggtttta
      901 cttcttcgcc gccgaggtat ttttctgcaa cgacatcatc gaaatcaccg agcgccgcta
      961 cgagctcttc tcggcgtttc ttagcttcgt ccatcaactc tgctggaatc gcttcctcac
     1021 gaattttttc gccattgtcg ccatcgaaat agaaggcctt catagcaacc aggtcaatca
     1081 caccttgaaa tttgtcttca tagccgatcg gcatggtgat tgcgtgggcg ttgtgaccca
     1141 gtttttcgcg caattgttga atgccgcgcc atgggttcgc accggcacgg tccattttat
     1201 tcacaaaaca aatg
//
LOCUS       DAOMXO010000726         1213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_67958_length_1213_cov_3.935233, whole genome shotgun sequence.
ACCESSION   DAOMXO010000726 DAOMXO010000000
VERSION     DAOMXO010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67958_length_1213_cov_3.935233"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..>1213)
                     /gene="aceB"
                     /locus_tag="PLH57_11885"
     CDS             complement(12..>1213)
                     /gene="aceB"
                     /locus_tag="PLH57_11885"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HRK03356.1"
                     /translation="EGTIQGQANLYEAVRRSLNFQSPEGKSYRLNDKVATLLVRPRGW
                     HLDEKHVMVDGSAASGSLFDFGLYFFHNAKELVDRGTGPYFYLPKLENHLEARLWNEV
                     FNEAQDLLGIPRGTIKATVLIETILAAFEMDEILYELRDHIVGLNCGRWDYIFSFIKK
                     FRNKPEFSLPDRAHITMTQHFLNSYSELLIQTCHKRGAFAMGGMAAQIPIKNNPEANA
                     AAMDRVLKDKQREVRMGHDGTWVAHPGLVELARGVFDQALTGPNQLNVLREDVRITAA
                     DLLKIPEGTVTEAGIRMNISVGVQYLEAWLRGQGCVPIHHLMEDAATAEISRAQLWQW
                     LRHGRLNAVQFQNFLSDEMKKIKETVAPSTWEASRFELAAELFSKITTASEFPEFLTL
                     SAYEHLA"
BASE COUNT          314 a          302 c          253 g          344 t
ORIGIN      
        1 tggctcttta ttcaagcgag atgctcgtaa gcagaaagtg ttaaaaactc cggaaactct
       61 gatgcagtag taattttcga aaaaagttct gccgcaagtt caaatcgact agcttcccaa
      121 gtgctaggag caactgtttc ttttattttt ttcatttcgt ccgacaaaaa attctgaaat
      181 tggaccgcgt ttaatcgccc atggcgcaac cactgccaca gttgtgctcg cgaaatctca
      241 gcagtagcag cgtcttccat taaatgatga ataggcacgc atccttggcc ccgcaaccac
      301 gcctcaagat actgaacccc cacactaata ttcatgcgaa ttccggcttc agttaccgtc
      361 ccttcgggga tttttaacaa atctgctgca gtaattcgca cgtcctcacg caaaacattt
      421 aattgattgg ggccggttag cgcttgatcg aatactcctc gagccagttc gaccaatccc
      481 gggtgcgcca cccaagttcc atcgtgcccc atacgcactt ctcgttgttt atcttttaat
      541 acccggtcca ttgccgcggc gttagcctct ggattgtttt taatcggtat ttgcgccgcc
      601 ataccaccca tcgcaaacgc tccgcgttta tgacaggttt gaatcaatag ttcagaatac
      661 gaattgagaa aatgttgggt catagtaatg tgcgcgcgat ctggcagcga aaactcgggt
      721 ttattccgga atttttttat gaagctaaat atataatccc aacgtccgca attgagacct
      781 acaatgtggt ctctaagctc atagagaatc tcgtccattt caaaggcggc aagaatagtt
      841 tctatcaata ccgtcgcttt gatcgtgccg cgaggaattc ccagaaggtc ttgagcttca
      901 ttgaagacct cgttccacag ccgagcttcc agatggtttt ctagttttgg taaataaaaa
      961 taaggtccag tacctcggtc tactagttct ttggcattgt gaaaaaagta gagtccgaaa
     1021 tcgaaaagac tgcctgaagc tgcgctaccg tcgaccatca catgtttttc gtctaaatgc
     1081 caaccccgag gtcgtacaag aagcgtcgca actttgtcgt tcagccgata agatttacct
     1141 tcgggacttt gaaaatttag agatcttcga acggcttcgt agagatttgc ttgcccttga
     1201 attgttcctt ccc
//
LOCUS       DAOMXO010000727         1200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_68978_length_1200_cov_3.852402, whole genome shotgun sequence.
ACCESSION   DAOMXO010000727 DAOMXO010000000
VERSION     DAOMXO010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68978_length_1200_cov_3.852402"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1114
                     /gene="pheS"
                     /locus_tag="PLH57_11890"
     CDS             53..1114
                     /gene="pheS"
                     /locus_tag="PLH57_11890"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRK03357.1"
                     /translation="MLLGISELEGECLKAIAVATTAEALQEVDLKYLSKKGLFSECMK
                     SLRDVSSEDRPKVGARANEAKTRIDAALTAKRNAFADEALNAQLQSEAIDATLPALRR
                     PPGSTHLITKVMGELVRIFQRLGFEIADGPEVETEFHNFDALNMLDDHPARDMQDTFF
                     VGGVDGKTAKATNQTGVVLRTHTSPVQIRTMLAKKEPPVRILAPGAVFRSDYDVTHSP
                     MFHQLEGLYVNKKVSFAELKGCLLFFAREYFGPNTQIRMRPSFFPFVEPGAEIDVSCV
                     MCSGKGCRVCKETGWLEILGGGMVHPSVFKAAGMDSRLVQGFAFGMGIERIAMLKYGV
                     PDLRLLFENDPRFLKQFGI"
BASE COUNT          296 a          277 c          332 g          295 t
ORIGIN      
        1 aggcagcggc gatcaaggct ggcatggtca ggcgggggcg gtggtgctac gtctgctgct
       61 gggcatctcc gaacttgaag gggagtgcct aaaagcgatc gcggttgcga ccaccgcgga
      121 ggccctccaa gaagtcgacc tgaagtatct ttcgaaaaaa ggtttgttta gcgagtgcat
      181 gaagtcgctt cgtgacgtct caagtgaaga tcgtccaaaa gtaggtgctc gcgctaacga
      241 agctaagacc cgtattgacg ctgcgctcac agcaaaacgc aacgcctttg ctgatgaagc
      301 gctcaatgcg caacttcaat cggaagcaat cgacgccact ctcccagctc tacgtaggcc
      361 gcccggatcg acacatttga tcaccaaagt aatgggtgaa ctggtgcgaa tttttcagcg
      421 ccttgggttt gaaatcgccg atggtccaga ggtggaaacc gaatttcaca attttgatgc
      481 gcttaacatg ctcgatgacc acccagctcg agatatgcag gatacattct tcgtaggcgg
      541 cgtcgatggt aaaacggcta aagctaccaa ccaaactgga gtagtactgc ggacccatac
      601 gtcgccagtt cagattcgaa ctatgttagc gaaaaaagaa ccgccggtca ggattctggc
      661 accaggagcg gtttttcgtt ctgactacga cgtcacacat tcgccgatgt ttcaccaact
      721 cgaaggcttg tacgtgaata agaaggtgag tttcgctgag ctaaagggct gtcttttatt
      781 ttttgcgcgc gagtattttg gtcccaatac ccaaattcgt atgcgaccga gcttttttcc
      841 gttcgtagag ccaggtgcag agatcgacgt gagttgcgtc atgtgttctg ggaagggctg
      901 ccgtgtttgc aaagaaactg gatggctaga aattttaggc ggcggcatgg tgcacccgag
      961 cgtgtttaaa gcagctggta tggattcgcg attggttcaa ggttttgcgt tcggaatggg
     1021 aatcgagcga attgccatgt tgaaatacgg agttcccgac ttacgactgc tgtttgaaaa
     1081 tgaccctcgt tttctcaaac aatttggaat ttaaacggtg acgaaatgaa aataagcgtt
     1141 aactggttgt ctaaatatat ggatgtcgca ccactgcgcg ccgacctttc gggggtgctc
//
LOCUS       DAOMXO010000728         1194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_69488_length_1194_cov_4.266023, whole genome shotgun sequence.
ACCESSION   DAOMXO010000728 DAOMXO010000000
VERSION     DAOMXO010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69488_length_1194_cov_4.266023"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PLH57_11895"
     CDS             complement(<1..148)
                     /locus_tag="PLH57_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRK03358.1"
                     /translation="MASELKELVEFLAKTLVDKPNEVEVREIEGEQTTVLELKVAKDD
                     IGKII"
     gene            complement(244..489)
                     /gene="rpsP"
                     /locus_tag="PLH57_11900"
     CDS             complement(244..489)
                     /gene="rpsP"
                     /locus_tag="PLH57_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRK03359.1"
                     /translation="MAVVIRLSRAGTKKKPFYHIVAADKIKSRDGKYLERLGCFNPRR
                     KGHKDHLVVDRDQIQAWVTKGAVMSETVGQLLENNSK"
     gene            complement(551..>1194)
                     /locus_tag="PLH57_11905"
     CDS             complement(551..>1194)
                     /locus_tag="PLH57_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRK03360.1"
                     /translation="HERVPLTGLVVSKLDGDARGGVALSAREVTGVPIVFAGVGEKIE
                     DLEAFHPDRMASRILGMGDVMALVEKARESISEEEAAESAGKMMSGQFSLEDFRDQMK
                     RLRSMGPLEGILKLMPGMGQAMAQMKNVDADKEMKKVEAMISSMTRQERKNPDLLNGR
                     RRLRIAEGSGTSVADINRFIKQFAEMQKMMKQMSKMGLAKGLMKGGLKGLLNR"
BASE COUNT          266 a          335 c          270 g          323 t
ORIGIN      
        1 cgataatttt tccgatgtcg tccttcgcaa ccttgagttc gagcacagta gtctgctcgc
       61 cttcaatttc tcgaacttcg acttcgttag gcttatcgac caaagttttt gctaaaaact
      121 cgaccaactc tttgagttca ctggccatga gttcggctcc tcaacccggt ccagcgaaaa
      181 cgcgtgcgga accgacgcag tatccagctt ttgttgagca cttcaactgg atctggttaa
      241 ggattatttt gagttattct cgagcagttg tccaacggtt tcagacatta ctgcgccctt
      301 ggttacccag gcttgaatct gatcgcggtc tacgacgagg tggtctttgt gacccttacg
      361 gcgcggatta aagcagccca agcgctctaa atatttaccg tcgcgagact taatcttgtc
      421 ggccgctacg atgtgataaa acggcttctt tttggtacca gcgcgggaca aacgaataac
      481 tacggccatg aaatcatctc cttggaggaa tctttaaagg atcttcttat gcccaaaaat
      541 ctgagtctag tcaacggtta agcagacctt taaggccgcc cttcattaaa cccttggcca
      601 ggcccatttt agacatttgt ttcatcattt tctgcatttc ggcgaactgc ttaataaagc
      661 gattgatatc cgcaacactg gtaccggacc cttcggcaat tcgcaggcgc cggcggccgt
      721 ttaaaagatc cgggttttta cgctcttggc gggtcatcga gctaatcatg gcctcaacct
      781 tcttcatctc tttgtcggca tcaacgttct tcatctgagc catcgcttgc cccatgccgg
      841 gcatgagttt taaaattccc tctagtggcc ccatcgatcg caaccgtttc atttgatcgc
      901 gaaagtcctc gagcgaaaac tgtccgctca tcattttgcc ggctgattcg gcggcttctt
      961 cttcggatat tgattcgcgc gccttttcca ccagcgccat gacgtcgccc atcccaagaa
     1021 tacgactggc catgcggtcg gggtgaaacg cttcgagatc ttcaattttc tcacctacac
     1081 cggcaaatac aattggtacc ccggtgacct cgcgagccga aagtgccaca ccaccgcggg
     1141 cgtcgccgtc aagcttactg acgacaagac cggtgagagg cacccgctcg tgaa
//
LOCUS       DAOMXO010000729         1191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_69718_length_1191_cov_5.945423, whole genome shotgun sequence.
ACCESSION   DAOMXO010000729 DAOMXO010000000
VERSION     DAOMXO010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69718_length_1191_cov_5.945423"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..913)
                     /locus_tag="PLH57_11910"
     CDS             complement(92..913)
                     /locus_tag="PLH57_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRK03361.1"
                     /translation="MENFGLIISVIGFAFMGTMLGLLGAGGSILSVPLLIYVIGLTPV
                     VATSYSLVIVAFCAAVALLTYLKAGLVDFKVAAVFAPLTVAGVWFARKILLPAVPDSI
                     ALLGTSVSKDVVILTVFGLVMLRAAWVMLVPKTKNKSPILQKNRTLLFIITGVRGLFL
                     GVLTGFVGAGGGFMLVPAIHQWFRLPMDRAIGTSLVVIVTNASLGAIFSIHHFELPMF
                     WKLSGLLLAATAGIYLGGTVLRKKIDPKSLRPVFGWMIVIIAVYVLYDGISKILR"
     gene            complement(944..>1191)
                     /locus_tag="PLH57_11915"
     CDS             complement(944..>1191)
                     /locus_tag="PLH57_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03362.1"
                     /translation="LPTGDPRAHYESLQKIKVNVGSEFILLPGHDMNGGRASSWKTQL
                     QEFPALSQNYDEFYADSGSWTGPAPKKLRESLFVNFK"
BASE COUNT          319 a          294 c          326 g          252 t
ORIGIN      
        1 cgagtcagag ctctctggag cgatcctaca gttggctaaa agttcgacag cgatcgttta
       61 gccgggcaag gaggtatgcg gcagcttgaa atcaacggag aatttttgag atgccgtcat
      121 agagcacgta gacggctata atgacgatca tccaaccaaa caccgggcgt aggcttttgg
      181 ggtcgatttt tttgcgcaat acagtgccac ccaaatagat gccggccgtg gcggcaagga
      241 gcagcccaga gagcttccaa aacataggga gctcgaagtg atggatactg aatattgccc
      301 caaggctcgc attggtgaca atgactacca aagaagtgcc gatagctcgg tccattggca
      361 gacgaaacca ctggtggatg gctggtacga gcatgaatcc gccgccggcg ccaacgaaac
      421 ccgtgagtac gccgagaaat agcccgcgca ctccggtgat aatgaaaaga agcgtgcggt
      481 ttttttgaag aatgggggat ttgttttttg ttttaggtac taacatcacc caagcggcgc
      541 gcagcatgac taagccaaaa accgttaaaa tcacgacgtc tttggaaacg gacgttccaa
      601 gcagtgcgat cgagtccggc acggctggca ggagaatttt gcgggcaaac cagacccctg
      661 caacagttag tggggcaaaa acagcagcga ctttgaagtc caccaagccc gcctttaggt
      721 aggtgagcaa ggcaacggcc gcacagaagg ctacgatcac gagcgaataa ctcgtagcaa
      781 ccacgggcgt aaggccgatc acgtagataa gaagaggcac gctaaggata gacccacctg
      841 cgcctaacaa gcccaacatg gtgcccatga aggcaaatcc gattaccgaa atgatgagtc
      901 cgaaattttc cataggtgcc tcgttcttgg atgtacgctc gtattattta aagttcacga
      961 acaacgactc gcgcaatttc ttgggcgcag gtccggtcca gctcccggaa tctgcgtaga
     1021 actcgtcgta gttctgagaa agcgccggaa actcttgaag ttgcgttttc caactcgagg
     1081 ctcggcctcc gttcatgtca tgtccgggca gtagaatgaa ttcgcttcca acgttaacct
     1141 taattttttg gagactctcg taatgtgcgc gtggatcgcc ggttggcaga t
//
LOCUS       DAOMXO010000730         1190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_69821_length_1190_cov_4.088106, whole genome shotgun sequence.
ACCESSION   DAOMXO010000730 DAOMXO010000000
VERSION     DAOMXO010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69821_length_1190_cov_4.088106"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLH57_11920"
     CDS             complement(<1..192)
                     /locus_tag="PLH57_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HRK03363.1"
                     /translation="MASSVNLFNTGKSGLFASRTALSTTGHNIANANTEGYSRQRVDQ
                     TTSTPIGLGNVVLGTGTRVA"
     gene            complement(199..720)
                     /gene="flgN"
                     /locus_tag="PLH57_11925"
     CDS             complement(199..720)
                     /gene="flgN"
                     /locus_tag="PLH57_11925"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HRK03364.1"
                     /translation="MESRLNELQYILEKLLSVHRTLLDVLDEEHAHMVSLEIEALGEA
                     SKTKEVLLAEIWNLEQLRKKIVDEISIECKLNPATVTLKELSLALGQRIEGPGITVLV
                     KTREALNMLIEQARDRNKRNMTFAEESLARIEELKRNVLGVASSSSPENYSSQGTRQP
                     IAEQGGRLLSTEA"
     gene            complement(767..1078)
                     /gene="flgM"
                     /locus_tag="PLH57_11930"
     CDS             complement(767..1078)
                     /gene="flgM"
                     /locus_tag="PLH57_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRK03365.1"
                     /translation="MKVNPAASNAIEKTKDSTATNRAGEAQTSKNKRKQSLFGGGANY
                     EKVDISSGAKQAAKAKEIASNAPDVREERVAQLREAIQKGTYKVDTDAVAEKMLAEHM
                     R"
BASE COUNT          257 a          286 c          262 g          385 t
ORIGIN      
        1 tgctactcgg gtgcccgtac ccaaaacaac attacctaat ccaatcggtg tgcttgttgt
       61 ttgatcgact ctttggcgag agtatccctc agtattggcg ttggcgatat tatgaccggt
      121 agtagacagt gcagtacggc tggcaaaaag tccggacttt ccggtattaa aaagattaac
      181 ggaacttgcc atttttgttt aagcttccgt tgaaagaagc ctacccccct gctccgcaat
      241 tggttgccgg gtaccctgac tcgaatagtt ttctggacta ctcgaactgg ctacgcccaa
      301 cacattgcgc tttaattctt caattctagc gagagattct tcggcaaatg tcatattacg
      361 tttattgcgg tctcgggctt gttcaatgag catgtttaag gcttcgcggg tttttaccaa
      421 caccgtgatc cctgggcctt caatgcgttg tcctagagcc aacgatagct ctttgagagt
      481 aacggtggct gggttcagct tgcactcaat cgaaatttca tcgactattt tttttctaag
      541 ttgttcgagg ttccatattt cagcaagcag cacttcttta gttttagagg cctcgcccaa
      601 ggcttcgatc tcgagcgaca ccatgtgcgc gtgctcttca tctaaaacgt ctagcaacgt
      661 tcgatgaacg ctaagtagtt tttctagaat atactgtaat tcgtttagcc tagattccat
      721 ggcgttacgt ccggccctcc tagccgggca aacttagggt gcgaacttat ctcatatgtt
      781 cagcgagcat tttctccgcg actgcatcgg tatcaacttt gtaggtgcct ttttgaattg
      841 cttcgcgcaa ttgcgctact cgctcttctc gaacatctgg ggcattggat gcgatttctt
      901 tcgccttggc tgcctgttta gcacccgaag aaatatctac tttttcgtaa ttagctccac
      961 cgccaaataa actttgtttt cttttatttt tcgatgtttg tgcttcacct gcgcgattgg
     1021 tagcagtgga atctttggtt ttttctatcg cgttggaagc tgccggattt accttcataa
     1081 gacctccgtg tctctataca taaagttatc ttttacgtcg aatctgcgca aggaccaggt
     1141 cctttatgcc taacatgccc tgggagctca ttgtgcggga atattcattg
//
LOCUS       DAOMXO010000731         1188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_69956_length_1188_cov_4.963813, whole genome shotgun sequence.
ACCESSION   DAOMXO010000731 DAOMXO010000000
VERSION     DAOMXO010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69956_length_1188_cov_4.963813"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLH57_11935"
     CDS             <1..300
                     /locus_tag="PLH57_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRK03366.1"
                     /translation="NVESFLKLHGLDFFDFIQGGSSVLGKARILERILAEKNMDPATT
                     VYVGDEARDIVAARTVGVRSVAVTWGFNSRRLIEAATPDRIISKPIELLNLGHLY"
     gene            339..>1188
                     /locus_tag="PLH57_11940"
     CDS             339..>1188
                     /locus_tag="PLH57_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03367.1"
                     /translation="MRLRSIIIYSVFLHGLLLFGAFVHAQDSSTSLSNLQTVSVDSIN
                     IKASVSLGSDRPPGEFTFRPLDAFWVWRKAHQYSEVGTPAVRVVFSDGLDQRLLLDSV
                     IEEQDVMLVSRKQRKITVDLLGSSARLKVRVASQGSVYTFELQFQLDLEVPQLVRGST
                     CENYGVDLIRREGPSGFPFLGVDCLEEENAIAVGIVRPGGTSWVASSDADRLNRVFRR
                     TVKKSGFLGNDLHLRLVLPDGKAASFHLRVSEKKPANSPIFVPTFGGQLAARSFSGSR
                     QLDDRRW"
BASE COUNT          266 a          269 c          326 g          327 t
ORIGIN      
        1 aacgttgaat cttttttaaa gttgcacggt ctagattttt tcgactttat ccaaggtggt
       61 tcaagtgtat tgggtaaagc gagaattctg gaacgcatcc tcgcggaaaa aaacatggat
      121 cccgccacca cagtatacgt tggcgacgag gccagagaca tagttgcggc acgaaccgta
      181 ggcgtgcgat cggtagcggt gacttggggg tttaactcgc ggagactcat tgaggcggct
      241 accccagatc gaataatatc caaaccaatc gaattgttaa atttgggaca cctgtattag
      301 gatcgcagga gacggctatt ggtcgggggg ccagcatctt gagactgcga tctatcatta
      361 tatacagcgt ttttctgcac gggttgcttt tgtttggggc ctttgtgcat gcccaagatt
      421 cttcgacttc gctttctaat cttcagactg tttcggtgga ttcaattaat attaaggcga
      481 gtgtctcttt gggatcggat cgccctcccg gagaatttac ctttcgcccc cttgatgcgt
      541 tttgggtttg gcggaaggcc caccagtatt cagaggttgg cacgcccgcg gtgcgcgttg
      601 tttttagtga cggactcgac caacgccttc tgctcgattc agttatagaa gaacaagacg
      661 tcatgctggt gtcccgtaaa caacgaaaaa ttacggtgga ccttcttggc agctccgcac
      721 gtctcaaagt tcgcgtcgcc agtcagggtt cggtctacac tttcgagctc cagtttcagt
      781 tggatctaga ggtgccgcaa ctagtccgag gtagcacttg cgaaaattac ggagtcgatc
      841 tcattcgacg tgaaggacca agtgggtttc cctttttggg ggtagattgt ctcgaagagg
      901 aaaatgcgat tgccgttgga atcgtgcgac ctggaggtac gagttgggtt gcctctagcg
      961 atgctgatag gcttaatcga gtttttcgtc gcacggttaa aaaaagcggt tttcttggta
     1021 acgatttgca cctgagactt gttttgccag atggcaaggc ggccagcttc cacttaagag
     1081 tgagtgaaaa gaaacccgct aacagtccga tattcgtacc cacgtttggc ggtcagctcg
     1141 cagcacgatc tttttcgggt tcgcggcaac tcgatgatcg gcgttggt
//
LOCUS       DAOMXO010000732         1187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_70036_length_1187_cov_4.686396, whole genome shotgun sequence.
ACCESSION   DAOMXO010000732 DAOMXO010000000
VERSION     DAOMXO010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70036_length_1187_cov_4.686396"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /gene="rplI"
                     /locus_tag="PLH57_11945"
     CDS             <1..348
                     /gene="rplI"
                     /locus_tag="PLH57_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRK03368.1"
                     /translation="PRLSDSQALRSATDHHHRALARKREKNTKDATELATKLEAYSVT
                     IRCKVGENDKLFGSVTAADIAAALAKGGYVIDKRQVQIDDPIKQAGVATVKVKLSPEI
                     SAQVKVWVVGDNA"
     gene            396..>1187
                     /locus_tag="PLH57_11950"
     CDS             396..>1187
                     /locus_tag="PLH57_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HRK03369.1"
                     /translation="MEFGDEDNRYGGAPSFGTGSSSGTNSADSRHVPPHSLEAERSVI
                     GALLIDSAAYAKVVDIGLQPIDFYRESHGKIFEAIQDLTARSEPADLVTLTTTLKNRG
                     TFDLVGGSSYLTGLFEDAYSSAHITHYSKIVKEKAILRRLISTANELVGTAFSGVDDV
                     EEFLDHAEKAVFEVTDSKLRQSFVGIKQILMENMQSIEELAQKKATVTGLPTGFTELD
                     RMTSGLHPGNLVIVAGRPAMGKTSLVLNLAENAALQGKASVALFSL"
BASE COUNT          309 a          277 c          303 g          298 t
ORIGIN      
        1 ccccgactat ctgattccca agcccttcga tccgcgactg atcaccacca tcgcgcactc
       61 gctcgtaagc gcgaaaagaa caccaaagat gcaacggaac ttgctaccaa gctcgaagcg
      121 tattcggtta ccattcgttg caaagtcggc gaaaacgaca agctctttgg ttcagtaacg
      181 gcggctgaca ttgctgcagc acttgcgaag ggcggctatg ttattgataa gcgacaagtt
      241 caaattgatg acccaattaa gcaggctgga gtggccactg tgaaagtaaa actttcacct
      301 gaaatttcgg ctcaagttaa agtttgggtc gtgggcgata acgcttaaaa aattcggtcg
      361 aataactttt ttcaaaacag gtagtcgggg gacccatgga gtttggtgac gaggacaacc
      421 gttatggcgg tgcaccttcg tttggcacag gatcatcgag cggaacaaac tctgcggatt
      481 cgcgccatgt gccgccccat agtctagagg ctgaacgttc ggtaatcggt gcgttgctga
      541 tcgactcagc cgcatacgcc aaagtggtcg acattggttt gcagccgatt gatttttatc
      601 gtgaatctca cggaaaaatt tttgaagcca ttcaggacct cacggcacga agtgaacccg
      661 ctgatttagt tactcttacg acgacactta aaaatcgagg aacttttgat ctcgtcggcg
      721 gctcctctta tttaacgggt ctttttgaag acgcttatag tagcgcacac atcactcatt
      781 attcgaaaat cgtcaaagag aaggcgatac ttcgccgtct catatcaact gctaacgagt
      841 tggtaggaac tgcctttagt ggtgtggacg acgttgagga gtttttggat cacgccgaga
      901 aggcggtgtt tgaggttaca gactccaaac tgcgacaaag cttcgtaggc attaaacaga
      961 ttttgatgga aaatatgcag tccatcgaag agttggcgca aaaaaaagcg actgttaccg
     1021 ggctaccgac tggattcacc gaacttgacc gcatgacctc tgggctgcac ccggggaact
     1081 tggtgattgt tgcaggtcgt cctgcgatgg gtaaaacctc gctggtgtta aatttagctg
     1141 aaaatgcggc ccttcaaggt aaagcctctg tcgcattgtt ttcgctg
//
LOCUS       DAOMXO010000733         1186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_70107_length_1186_cov_6.401415, whole genome shotgun sequence.
ACCESSION   DAOMXO010000733 DAOMXO010000000
VERSION     DAOMXO010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70107_length_1186_cov_6.401415"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLH57_11955"
     CDS             complement(<1..744)
                     /locus_tag="PLH57_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRK03370.1"
                     /translation="MTRSIDLEIKVGIFVAFGVGLVLFTIMLLGGGRTLFEKGTTFSA
                     KFPQVEGLVEGATVKVAGVKVGQVKTIEFQSEHNLVNVTFSVNEQFASAVREDSTVGI
                     QTQGVLGDRYIVVNPGGLSSKAAADGTELKAEHPKDLKDYLSNADEVLDRVKNTLKNA
                     EAILTNFNRDNRSDLFFKNLTEMTENLSGATDGLPTKMKDVTTAAKHMKSILAKIDNG
                     EGTVGALINDPSLYDDVKNLLGGANRNKVL"
     gene            complement(741..>1186)
                     /locus_tag="PLH57_11960"
     CDS             complement(741..>1186)
                     /locus_tag="PLH57_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK03371.1"
                     /translation="ENLAYPLMEHTKLSPEQIDARIKEMLELIGLKGSEKLLPASLSG
                     GMQKRVGAARAIIMKPKVILYDEPTAGLDPFNTQRLLDLMKRIQKREGNTAVFVTHDI
                     PSAFEVADRIAIMYNGKIHTLDTTENVKRSKDPFVQAFLAGEEIK"
BASE COUNT          267 a          281 c          294 g          344 t
ORIGIN      
        1 caggaccttg ttgcgattgg ccccacccag taggtttttt acgtcgtcat agagcgacgg
       61 atcgttgatc aaggcaccca cggtaccttc accattgtcg attttggcca aaatggactt
      121 catgtgtttg gcggcagtgg taacgtcttt catcttggtg ggcaacccgt cggtagcccc
      181 cgagaggttt tctgtcatct cggttagatt tttaaaaaat aagtcagaac gattatcgcg
      241 gttaaaattt gttaatatcg cctcagcatt tttgagcgta ttttttacac gatcgagcac
      301 ttcatcggcg ttactgaggt agtccttcaa gtccttcgga tgttcagctt ttagctctgt
      361 gccatcggcg gccgctttgc tggagagtcc gccgggattg acgacaatgt agcgatctcc
      421 gagtacgccc tgggtttgaa tgccgactgt cgaatcttct cgaacggcac tggcaaattg
      481 ttcgttcacg ctaaacgtaa cattcaccag attgtgttcg ctttgaaatt caattgtttt
      541 cacctggcca acttttacgc ctgcgacttt cacggtagcg ccttcgacta gaccttcgac
      601 ttggggaaat ttggctgaaa aagtggtacc tttctcgaaa agagtgcggc cgccaccgag
      661 tagcatgatc gtaaaaagta ccaatccaac accaaacgct acaaatattc cgaccttaat
      721 ttctaaatca atcgagcgtg tcatttgatc tcctcaccag ccaagaacgc ctgaacaaaa
      781 ggatctttgg atcgttttac gttttctgtt gtgtcgaggg tgtgaatctt tccgttgtac
      841 atgattgcga tacggtcggc gacttcgaaa gccgacggaa tatcatgagt gacgaatacc
      901 gcggtgttgc cctcgcgttt ttgaatgcgt ttcatcagat cgagcaaccg ctgcgtattg
      961 aacgggtcga gcccggcagt cggttcgtcg tacaaaatga ccttgggctt catgataatc
     1021 gcgcgcgcgg cgccaactcg tttttgcatg ccaccggaaa gtgaagcggg taagagtttt
     1081 tcgctgcctt taagaccgat tagttcgagc atctctttta ttcgcgcgtc gatttgttcg
     1141 ggggatagct tggtgtgttc cataagagga taagcgaggt tttcat
//
LOCUS       DAOMXO010000734         1182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_70479_length_1182_cov_4.716060, whole genome shotgun sequence.
ACCESSION   DAOMXO010000734 DAOMXO010000000
VERSION     DAOMXO010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70479_length_1182_cov_4.716060"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1182
                     /locus_tag="PLH57_11965"
     CDS             <1..>1182
                     /locus_tag="PLH57_11965"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HRK03372.1"
                     /translation="YTTIDNLIPGGSAERSGELQPKDKIIAVAQGEKGSFESVIDLQL
                     RDVVKLIRGKKDTIVRLTVLREEGGEAKRLIVSLKRDKINLQDEAAKLTYTTRKIGDK
                     SQKIAVIDLPSFYGDMSRKTRSSYEDLKKLVDQAVREKADGIVLDLSTNAGGLLSEAV
                     RITGLFISKGNVVATKGPNQKLDALDDDDDKIAYSGPLVVLTSRLSASASEIVSGALQ
                     DYKRALIVGGDHTFGKGTVQAMMNLPAGLGAIKVTTGMFYVPSGNSTQMRGIPADIVL
                     PSVFSTKDMGEVSLDYALPAHKVDPFLSPEATQKLASTVSSELIAKLRAKSEARVSKD
                     PEFKKIKDEVAEFEKKKGVVKLSDSLKKQKEENIKEKKEKKVAKKSRRRQSDEDYLKT
                     P"
BASE COUNT          358 a          295 c          274 g          255 t
ORIGIN      
        1 ctacaccacg atcgacaatt taatccccgg tggctccgcc gaacgatccg gcgagttgca
       61 acccaaagat aaaatcattg ccgttgctca aggtgaaaaa gggagtttcg aatcggttat
      121 tgacctccaa ttacgagacg tcgtgaaact cattcgcggg aaaaaagata ccatcgtacg
      181 tttaactgtt ctacgcgaag aaggcggcga agcgaaacga ctcatcgttt ctctcaaacg
      241 cgacaaaatc aatctgcaag acgaagccgc caaactcacc tacaccactc gcaagatcgg
      301 cgataagtct caaaaaatcg cagtcatcga cctgccgtcg ttctatggcg atatgagtcg
      361 caagacccga tcttcctacg aagacctcaa aaagctggtc gaccaagcgg taagagaaaa
      421 ggccgacggc attgtattgg acctttcaac caatgcaggt ggtttactgt ccgaggcggt
      481 tcgcattact ggtttattta tctcgaaggg taacgtcgta gctaccaaag gtcccaatca
      541 gaaactagac gcgcttgacg acgacgacga taaaatcgcc tatagtggtc cccttgtcgt
      601 tcttaccagc cgtctttctg cgagcgcttc tgaaattgtt tccggtgctc tacaagatta
      661 taaaagagcc ctcatcgtgg gtggcgatca tacttttggc aaaggcacgg tgcaggcgat
      721 gatgaacctc cctgcgggac tcggtgctat taaagtaacc accggaatgt tttacgtacc
      781 tagtggcaac tccacccaaa tgcgggggat tcctgccgac atcgtattac ccagtgtttt
      841 tagcaccaag gatatgggcg aggtcagcct tgattacgcg ttgccagccc ataaagtaga
      901 cccattttta agccctgagg ccactcaaaa acttgcttcg actgtttctt cggaacttat
      961 cgcaaagctt cgtgccaaat ccgaagctcg cgtgagcaaa gatccggagt ttaaaaagat
     1021 caaagacgaa gtggcggaat tcgaaaagaa aaaaggcgtc gtcaaactga gcgactcgct
     1081 aaaaaaacaa aaagaagaaa acattaaaga gaagaaagaa aagaaagtcg ccaagaaatc
     1141 tcgccgacgc caaagcgatg aagactatct gaaaacgcct ca
//
LOCUS       DAOMXO010000735         1181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_70579_length_1181_cov_3.939609, whole genome shotgun sequence.
ACCESSION   DAOMXO010000735 DAOMXO010000000
VERSION     DAOMXO010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70579_length_1181_cov_3.939609"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLH57_11970"
     CDS             complement(<1..572)
                     /locus_tag="PLH57_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03373.1"
                     /translation="MTKQKIYFYSFDKQLFEQMRGTIPGEVADLVWVQSEQQEWCAKQ
                     RHLTIVDGKRNDLRARLEELSHKKAYVVLLLNEDIAETQLEGDLGASLENGLVDDILL
                     TPIRTVEVLSKMKHVAHLARVDDVNLANEQLKFVIGEMEADLLLARGIQKHLIPEKFK
                     QVPGFQVNHKYLSGFKAGGDYLDFFEFEDST"
     gene            complement(569..>1181)
                     /locus_tag="PLH57_11975"
     CDS             complement(569..>1181)
                     /locus_tag="PLH57_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03374.1"
                     /translation="SFRVFNGIFRFGFSSQLLTIGDADTVILPPISSGNLDFKRSINA
                     GTGLVHTGGITLTLPVRFLPSFSGVYRNIGTAKFTRAPMVKFGDGRTIENKKPIIDLA
                     SSFTVYLAKRLETKWEIDFRDSTNRLKGNKMQHISFGTEWLFFDLLRLRGGVGHGYYS
                     AGLGLHTPRAQMDLAWYSDELDNQFRSRRDQRVVLQYTWGIFK"
BASE COUNT          321 a          294 c          246 g          320 t
ORIGIN      
        1 gtgctgtctt caaattcgaa aaaatcgaga taatctccgc ccgctttaaa accgctcaaa
       61 tatttatggt tcacctgaaa ccctggcact tgtttgaatt tttctggaat caagtgtttt
      121 tgaattccgc gggctagaag cagatccgct tccatttcgc caatcacaaa tttcagttgc
      181 tcgttcgcaa gattcacgtc gtcgacccga gctaaatggg cgacgtgctt cattttgcta
      241 agcacctcta ccgttcgaat tggcgtcaaa agaatgtcgt ccacaaggcc gttttcgagg
      301 cttgcaccca agtccccttc taactgagtc tctgcgatat cttcgtttag cagtaagact
      361 acgtaggctt ttttatgaga aagttcttct aaccgagctc ttaaatcgtt tcgcttacca
      421 tcaacgatcg tcagatggcg ctgttttgcg caccactcct gctgctcgga ttgaacccag
      481 accaagtctg caacctcacc aggaatagtt ccgcgcattt gctcaaacaa ttgtttatcg
      541 aatgagtaaa aatatatttt ttgttttgtc atttgaagat cccccaggtg tattgcaata
      601 cgacgcgttg atctcggcgc gaccgaaatt ggttgtctag ctcatcggag taccaggcta
      661 gatccatttg ggcacgcgga gtatgtaatc caagtccagc cgaatagtag ccatgtccaa
      721 ctccccctcg caggcgaagc agatcaaaaa acaaccactc ggttccaaac gaaatgtgct
      781 gcatcttatt gcctttcaat cgattggtgg agtcacgaaa atcgatctcc catttagtct
      841 caaggcgttt ggccagataa acggtaaaag aggacgccag atcaataatt ggttttttat
      901 tttcgatcgt tcgcccatct ccgaacttta ccattggcgc tcgagtaaat ttggcggttc
      961 caatattgcg gtagactccc gaaaaactcg gaagaaatct aaccggtaaa gtaagggtga
     1021 taccgccagt atggaccaaa ccggtacctg cgttgatgct gcgcttaaag tctaaattac
     1081 cgctagaaat cggcggtaat ataaccgtgt ccgcgtctcc gatggtcaac aactgactcg
     1141 aaaatccaaa acgaaaaatc ccattaaaca cacgaaacga a
//
LOCUS       DAOMXO010000736         1179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_70779_length_1179_cov_2.500890, whole genome shotgun sequence.
ACCESSION   DAOMXO010000736 DAOMXO010000000
VERSION     DAOMXO010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70779_length_1179_cov_2.500890"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PLH57_11980"
     CDS             <1..919
                     /locus_tag="PLH57_11980"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRK03375.1"
                     /translation="LRITTKELAAEKKVVFQERQLRVESNVMAQAFEKLNERLFLGHP
                     YGRPVIGYKEDLENATIQDCLQFFEQHYLPENALVVVAGNFDFKQTRKWIRDFYLSKS
                     NSKIKKFAKAVVPPAPQLEKSEAVTVRHEVASDSLLIGYPLPSMYHDDAEALSMAAWY
                     LFGMASSPMVDRMVRKTQVATSVNGSASFEKHGGAFLVNVDLRNGISGEQGLNLLDQV
                     LNDSMEDGISNEALQNMKRKMLYSLFNEGKSVVGVADWFGSGLFYWNDPGRMRAVFEK
                     YQDMTGESLERVIRKYLLGAKRVVVKVTR"
     gene            916..>1179
                     /locus_tag="PLH57_11985"
     CDS             916..>1179
                     /locus_tag="PLH57_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03376.1"
                     /translation="MMLARDRIISVIVFLILIAASFGKEGSAFASIVPKHFVVKTNNG
                     IEITVFPIAKVPIFDVELFFQNGANTEPLGRSGASSLMASLLNR"
BASE COUNT          330 a          254 c          285 g          310 t
ORIGIN      
        1 ccttcgcatc actaccaagg aactcgctgc agaaaaaaag gtagtctttc aagagcgaca
       61 gctacgagta gaaagtaacg tcatggctca ggcgttcgaa aaactcaacg agcgactgtt
      121 tttaggtcat ccttatggcc ggccggtcat cggttataaa gaagaccttg aaaacgccac
      181 gatccaggac tgcctgcagt tttttgaaca acactatcta cccgaaaatg cattggtcgt
      241 cgtagctgga aattttgact tcaagcaaac cagaaagtgg atacgtgatt tctatctttc
      301 gaaatcaaat tcgaaaataa aaaaattcgc caaagcagtg gtcccacccg cgcctcagtt
      361 ggaaaaatct gaagccgtga ctgtgcgtca cgaagtggca tccgattctc ttctaattgg
      421 gtatccatta ccgtcgatgt atcacgatga tgctgaggcc ctgtcgatgg cagcctggta
      481 tttatttggt atggcctcca gtccgatggt ggaccgaatg gtgagaaaaa cccaagtcgc
      541 cacatctgtg aatggttcgg catccttcga aaaacacgga ggcgcctttc tagtgaatgt
      601 cgatctacgc aatggaatat ctggggaaca aggacttaat ttactggacc aagtgttgaa
      661 cgattccatg gaagacggaa tttcaaacga agcgttgcaa aatatgaaaa gaaaaatgct
      721 ttatagtctt ttcaatgaag gcaaaagcgt ggtcggcgtg gctgattggt ttgggtcagg
      781 tctgttttac tggaacgacc ccggtcgcat gcgtgcggtg tttgaaaaat atcaagatat
      841 gaccggtgaa agcctcgaac gcgtaattcg caaatattta ctgggcgcca aaagagttgt
      901 cgtgaaggtt actcgatgat gctcgcgaga gatcgaataa ttagcgttat agtttttcta
      961 atcttaatcg ccgcttcgtt cggcaaagag ggtagcgctt ttgcttcgat cgtgcctaaa
     1021 cactttgtag ttaaaaccaa caacggaatc gaaataacag tttttcccat cgccaaggtt
     1081 ccaattttcg acgttgaact tttttttcaa aacggggcca atacggaacc cttggggcga
     1141 tcgggtgctt caagtcttat ggcgtcgctt ttgaatcgc
//
LOCUS       DAOMXO010000737         1176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_70974_length_1176_cov_7.403211, whole genome shotgun sequence.
ACCESSION   DAOMXO010000737 DAOMXO010000000
VERSION     DAOMXO010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70974_length_1176_cov_7.403211"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..193)
                     /locus_tag="PLH57_11990"
     tRNA            complement(118..193)
                     /locus_tag="PLH57_11990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Thr,seq:ggt)
     gene            complement(229..305)
                     /locus_tag="PLH57_11995"
     tRNA            complement(229..305)
                     /locus_tag="PLH57_11995"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(269..271),aa:Gly,seq:tcc)
     gene            complement(316..401)
                     /locus_tag="PLH57_12000"
     tRNA            complement(316..401)
                     /locus_tag="PLH57_12000"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(365..367),aa:Tyr,seq:gta)
     gene            complement(414..489)
                     /locus_tag="PLH57_12005"
     tRNA            complement(414..489)
                     /locus_tag="PLH57_12005"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(454..456),aa:Thr,seq:tgt)
     gene            complement(579..>1176)
                     /gene="rlmB"
                     /locus_tag="PLH57_12010"
     CDS             complement(579..>1176)
                     /gene="rlmB"
                     /locus_tag="PLH57_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRK03377.1"
                     /translation="KQAEKAGVRVEITVKNEGDEGCSALLSDYQYTDFDDFCAQLKSE
                     IDAGERPCVLMLDGVTDPQNLGAILRTAAFMNVAGVVLPKDRASPITDTVYRVASGGL
                     EYVPVCQVPNLVSAMARLKECGFWSVGFSEHAKQTLAAVRRDIAPLLVIGNEEKGIRP
                     LVRENCDFLAKFEGRGKLASLNASVAAALAMQWAVSEV"
BASE COUNT          287 a          311 c          270 g          308 t
ORIGIN      
        1 cgtaccaatg ttacagtgcg gcttgttccg ctcaaatttc tcttttgaca taaacttttc
       61 ttaccctttc tatttctcgt ttaaatatcg ccaatatttg gaagtctaaa ttttaaatgg
      121 tgctaatgat cgggatcgaa ccgatgacct cacccttacc aagggtgtgc tctaccaact
      181 gagctacatt agcaactcgg gcaaacagga caacttgcgc taaaattttg gagcgggaga
      241 cggggctcga acccgctacc ctcagcttgg aaggctgatg ctctagccaa atgagctact
      301 cccgctctaa ttttatggtg gagggagaag gattcgaacc ttcgaaggca taagccgaca
      361 gatttacagt ctgttccctt tggccacttg ggtatccctc cagccaaata aaatggtgct
      421 ggcgatggga ctcgaacccg caacctgctg attacaaatc agctgctcta ccaattgagc
      481 tacaccagca caagtctcta ctcgcacttg ttgcagtcag gtagagcgcg ctcgaggtta
      541 tatttttttt cgctgaaatg gcaataagtt tttaaagttt aaacttcgga cacggcccac
      601 tgcatggcaa gggccgcggc gacggaggca tttagggacg cgagtttgcc tcgcccctcg
      661 aattttgcga gaaaatcgca gttttctcgg accaacggtc gaattccttt ttcttcgttc
      721 ccgattacca ataatggggc tatgtctcgt cgtaccgccg cgagcgtttg ttttgcgtgc
      781 tctgagaaac ctacgctcca aaaaccacat tccttaagtc tcgccatggc tgaaacaaga
      841 ttgggaacct ggcacaccgg gacgtactct agccctccgg acgcaacacg gtagaccgta
      901 tcagtaatcg gtgatgcccg gtcttttggt aaaaccacac ccgcaacatt catgaacgca
      961 gcagtacgca aaatcgcccc caagttttga gggtcagtaa cgccgtcgag catgagaaca
     1021 cagggccgct ccccagcgtc gatctcgctt ttcagttggg cacaaaaatc atcgaaatcg
     1081 gtgtattgat agtcggaaag aagggcgctg caaccttcgt cgccctcatt ttttacagtg
     1141 atttctacgc gtacccccgc tttttcagct tgtttt
//
LOCUS       DAOMXO010000738         1171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_71416_length_1171_cov_3.247312, whole genome shotgun sequence.
ACCESSION   DAOMXO010000738 DAOMXO010000000
VERSION     DAOMXO010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71416_length_1171_cov_3.247312"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLH57_12015"
     CDS             <1..577
                     /locus_tag="PLH57_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03378.1"
                     /translation="NIRHEYFFASRQLLIALPFLGWLTTLSISYFFSKRWKLRWVIGA
                     VFLVVGIILPLRHWVLNVPPFVDIPRYKMHETFNLPEFSDDKTIIVLSSCHVGSAYLY
                     SSNDGFKNYFDHYAAKNKMMVPVNKRVLLWSTAGNSCSGEIAKVPNDLNLLKLTKNNP
                     SQFAVLAPFNVAVPEAIRHLPCRTEINSQCN"
     gene            complement(574..1122)
                     /gene="fliW"
                     /locus_tag="PLH57_12020"
     CDS             complement(574..1122)
                     /gene="fliW"
                     /locus_tag="PLH57_12020"
                     /inference="COORDINATES: protein motif:HMM:NF009793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HRK03379.1"
                     /translation="MKIKTTRFEELEVSPEEIIAFPDGILGFTDLKRYCLVDPADETL
                     ILWMQSLDEPGVAFPVLEPRIFKSDYIVKLSAQELKLLCLENINQSIVFTILTIPRDV
                     TQMTANLKAPIVINLRDQVGRQVVLQENEFTLKHLMFKELKTHLTTIAATAKTRSETE
                     RKATSLPINLRSMVPSSQVKTL"
BASE COUNT          302 a          258 c          246 g          365 t
ORIGIN      
        1 gaatattcga catgaatatt tctttgcatc gaggcaactc ttgattgcgc ttccatttct
       61 gggttggctt actacccttt ccatttctta ttttttttca aaaaggtgga agctacgttg
      121 ggtgatcggc gctgtgtttt tggtcgtcgg gattattcta cctttgcgac attgggttct
      181 taatgttccg ccatttgtgg atattccgcg ttacaaaatg catgagactt ttaatttacc
      241 agaatttagc gacgacaaaa caatcatagt tctttcgagt tgccatgtgg gaagtgcgta
      301 tctttattct tcgaacgacg gctttaaaaa ttacttcgac cactacgcgg caaaaaacaa
      361 aatgatggtt ccagtcaata aacgcgtttt actctggtct acggcaggaa acagttgctc
      421 gggagaaatt gctaaagttc cgaacgatct taacttgctt aaactcacga aaaataaccc
      481 ctcacagttc gcagttcttg cgccgttcaa tgtggcggtg cctgaagcca ttcggcattt
      541 accctgtcgc actgaaatca actctcaatg caactaaagc gttttaactt gggaacttgg
      601 aaccatcgat cggagattga tcggtaggct tgtagctttc cgctctgttt ccgagcgggt
      661 tttagcggtg gcagcgatcg tagttaagtg ggtcttgagt tccttgaaca ttaaatgttt
      721 caaggtaaat tcattttcct gcaataccac ctgacgacca acttgatctc tcaaattgat
      781 tacgattgga gcctttaaat ttgcagtcat ttgcgtgacg tcacgaggaa tcgttagaat
      841 tgtaaaaacg atactttgat tgatattttc taagcaaagc agctttaatt cctgagcgga
      901 aagctttact atataatctg atttaaagat tcggggttca agcactggaa aagccacacc
      961 cggttcgtcc agtgactgca tccacagaat gagagtttca tccgccgggt ctaccaagca
     1021 gtatcgcttg agatccgtaa aacccaaaat tccatcggga aacgcgataa tttcttcggg
     1081 actaacttcc aattcctcaa aacgcgtcgt ttttattttc atgctcgacc tcctgtctcg
     1141 ctaaagcctg ggcgcaccat ctttttggtt t
//
LOCUS       DAOMXO010000739         1168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_71652_length_1168_cov_4.386343, whole genome shotgun sequence.
ACCESSION   DAOMXO010000739 DAOMXO010000000
VERSION     DAOMXO010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71652_length_1168_cov_4.386343"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..>1168
                     /locus_tag="PLH57_12025"
     CDS             192..>1168
                     /locus_tag="PLH57_12025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRK03380.1"
                     /translation="MNSFKDLSLPEALQSTLSAMNYSVPTPIQAKAIPIAMAGRDLVG
                     CAQTGTGKTAAFCVPMICSLIKTPAKTALILAPTRELAMQIEEVFKKLTARHQDLKIA
                     LLIGGASMGSQFRALRNRPRVIVATPGRLNDHLRQRSVTLDSVGILVLDEADRMLDMG
                     FAPQLNQILKFVPKNRQTLLFSATMPSDIQQLAMRYMQNPERVTIGTVSQPAVTIDQS
                     VVQTTQATKNNVLIDEVTSRDGSILIFARTKRRTDRLARFLYDSGHKVSRINGDRTQK
                     QRNDAIDGFKHGKFRILVATDIAARGIDISHIAHVINYDLPAVAEDYIH"
BASE COUNT          303 a          294 c          280 g          291 t
ORIGIN      
        1 ggcggcggtg gtcgtggcgg ccgtggtggc ttcggtggcg gcggcggtgg acgcggtcgt
       61 tactaagtcc taatttaatc aaggttctca aagggcctcc taaaaagggg gccctttgga
      121 aaccacacgc gactgctcgt ccctcctgac ttgattcgtc gcgtatctct tgcgacgaaa
      181 gcaaaaaata catgaattct tttaaagacc tgtctcttcc agaggcgctg caaagcacgc
      241 tctctgcaat gaattactct gttcctacac ctatccaagc caaggccatt ccaattgcaa
      301 tggctgggcg cgacctcgta ggttgtgcgc aaaccggaac cggtaaaacc gctgcttttt
      361 gcgttccgat gatatgcagc ctaataaaaa cacccgctaa aactgcacta attctcgctc
      421 cgactcgcga acttgcgatg caaattgaag aagtgtttaa aaaattaact gcgcggcacc
      481 aagatttaaa aattgcgcta ttgattggcg gcgcgtcgat gggttctcag ttccgagcgc
      541 ttcgaaatcg accgcgcgtg attgtcgcaa cccctggcag attgaatgat cacttgcgcc
      601 aaagaagcgt gacactcgat agtgtcggaa tccttgtttt agacgaggcg gatcgaatgc
      661 tcgacatggg gttcgctccc cagttgaatc aaattttgaa atttgtacct aaaaatcggc
      721 agacgctctt gttttcggcg acaatgcctt ccgatattca acaactggct atgcggtaca
      781 tgcagaatcc agaacgtgtg actattggca ccgtttctca gcccgcagtc acgattgacc
      841 aatcagtggt tcaaacgact caggcgacta agaacaatgt gctcatcgac gaagtcacat
      901 cacgtgacgg ctcaatttta atttttgctc ggaccaaacg ccgaaccgat cgcttggctc
      961 gttttcttta tgatagtggt cacaaagtaa gtcgtattaa cggcgatcga actcaaaaac
     1021 aaagaaatga cgcaattgac gggttcaagc atggaaaatt tcgaattctc gttgccactg
     1081 acattgcggc ccgtggaatt gacatctccc acattgctca cgtcatcaat tatgatctac
     1141 cggcggtagc ggaagattac attcacag
//
LOCUS       DAOMXO010000740         1166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_71798_length_1166_cov_6.559856, whole genome shotgun sequence.
ACCESSION   DAOMXO010000740 DAOMXO010000000
VERSION     DAOMXO010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71798_length_1166_cov_6.559856"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>1166
                     /locus_tag="PLH57_12030"
     CDS             59..>1166
                     /locus_tag="PLH57_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03381.1"
                     /translation="MPINTTYSSLLLKKLLSTLCILALFGFLTPNSDASVARLAVLGT
                     QPEFTNGSEAVYSPVVPGSLWYDDNFNMFYNPSHIMDHGSYATFHKGNITSGNNGGEG
                     GWVSTIGKQYAYGLYFNRGNSSAIGTDTTARLGAPGLNSSTNQVFAGNNNVLNTRRAL
                     DLLVGGDHGFKWGAKLTWAYNRDQSIATQAQDDLEATARYWHLALGGQYMGFEPFLGT
                     TFSTRFERNNNDRSAAATDVLEDMNIGLRYRYESWTPYLTYRHYRRSGNGPGISDPTT
                     TVGDTQVQGHMKIMGIGVGKSAMIAKGVDLLANLGFYYTPSGDSTNTNLIYREMKQYV
                     LPLNLGVEADVATWFTLRAGVEYYFWNEISYDSLG"
BASE COUNT          306 a          310 c          282 g          268 t
ORIGIN      
        1 acaaacactt caaatccaat tttaatcgaa ccacgtatta atcaatcttg agggacctat
       61 gccgatcaac acgacttatt catcgttact cctgaaaaaa cttttgtcga cactgtgtat
      121 cctagcgctg tttgggtttt taacgcctaa ctcagacgcc agcgtcgcgc gactcgcagt
      181 tctgggtacc caacccgagt tcacaaatgg ctccgaagcc gtgtattcgc cagtagtgcc
      241 cggctctctc tggtacgacg ataatttcaa catgttctat aacccgtcgc acattatgga
      301 tcacggaagt tacgcgacgt ttcacaaggg caacattact tctggcaaca atggcggcga
      361 agggggctgg gtttcgacga tcggaaaaca atatgcctat ggactctact tcaaccgcgg
      421 caatagctcg gcgattggca ccgacaccac ggcaagattg ggtgcaccag ggctcaacag
      481 tagcaccaac caagtgtttg ccggaaacaa caacgtgctc aatacgcgcc gcgcgttaga
      541 cttactggta ggcggagatc acggatttaa atggggcgca aaacttacgt gggcctataa
      601 ccgcgatcag agcattgcca cccaagccca agacgacctt gaagcaaccg cccgctattg
      661 gcacctagcg cttgggggac aatacatggg ttttgaaccg tttcttggta ctacgttcag
      721 cacgaggttt gaacgaaaca acaacgaccg atccgccgct gcgacggacg tactcgaaga
      781 catgaacatc gggcttcgtt atcgctacga gtcgtggact ccctatctca cttaccgcca
      841 ctaccgccga tccggtaatg gccctggaat ttcggaccca acaaccacgg tgggtgatac
      901 ccaggttcag ggtcacatga aaatcatggg tatcggcgta ggtaagtccg ctatgatcgc
      961 aaagggcgtc gatttactgg cgaacctcgg attttactac accccaagtg gtgatagcac
     1021 gaacaccaac ctgatctatc gcgaaatgaa acaatacgtt cttccattaa atctgggagt
     1081 cgaagcagat gtcgcgactt ggttcacgct tcgggctggt gttgaatatt atttctggaa
     1141 cgaaataagt tacgattcgc ttggca
//
LOCUS       DAOMXO010000741         1161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_72292_length_1161_cov_3.095841, whole genome shotgun sequence.
ACCESSION   DAOMXO010000741 DAOMXO010000000
VERSION     DAOMXO010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72292_length_1161_cov_3.095841"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLH57_12035"
     CDS             complement(<1..347)
                     /locus_tag="PLH57_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014012489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRK03382.1"
                     /translation="MSRLQEVRNRMQPILEKTLSDYLLNCEATGSRGCSAMTRYHFET
                     GGKRLRGVIPSAVFECFKRDPKEILYFGAAVEMIHNATLVHDDLQDGDELRRGRPTVW
                     KKYSVPQAINCGDA"
     gene            complement(344..>1161)
                     /gene="mvaD"
                     /locus_tag="PLH57_12040"
     CDS             complement(344..>1161)
                     /gene="mvaD"
                     /locus_tag="PLH57_12040"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HRK03383.1"
                     /translation="DELILNGSIESSEKITKVIAVLNEVRKLSGKSHKARVQSRNNFP
                     TAAGLASSASGLSAVALAAVRAAGLDIPASKISEIARRGSGSAARSIFGGFVQWKAGK
                     ELDGSDSVAENFKPASHWNLVVRVLVVRATAKTHASTSGMEHTRKTSPYFESWVNSAQ
                     RDVAEILNAIENKNFNELANIAESNCLRMHASAMAARPTVFYWQPQTLALIQEISDLR
                     RGGVPVFYTIDAGPNVVCFFEKDATDVVDRALERIEGVQILRTTIGEGARVLS"
BASE COUNT          299 a          305 c          234 g          323 t
ORIGIN      
        1 gcatcgccgc aatttattgc ctgaggaacg gaatattttt tccatacggt cggcctaccc
       61 cggcgcagtt cgtctccgtc ttgaagatca tcatgcacca aagttgcgtt gtgaatcatt
      121 tcgaccgcgg caccaaaata taaaatttct tttgggtctc ttttaaaaca ctcaaaaacg
      181 gctgacggaa taacaccgcg caatcttttt ccaccggttt cgaaatgata ccttgtcatt
      241 gctgaacaac ctcttgaacc agttgcctcg caatttaaca aataatcact tagtgttttt
      301 tccagaattg gttgcatgcg attacgcact tcttgcaatc gactcatgaa agaacccttg
      361 ccccttcgcc gatcgttgtt cgaagaattt gcacaccttc aattcgctcg agggcgcgat
      421 ctacgacgtc cgttgcgtct ttttcaaaaa aacaaacaac attcggaccc gcgtcaatgg
      481 tgtagaacac cggtacgcct ccgcggcgca gatcgctaat ttcttgaatc aaagctaaag
      541 tttggggctg ccaataaaac actgtaggac gagcggccat tgctgaagca tgcatgcgaa
      601 gacaattcga ctccgcaata tttgctagct cattaaaatt tttattttcg atggcattca
      661 atatttcagc cacatcgcgc tgggctgagt taacccagga ctcaaaataa ggagatgttt
      721 ttcgtgtgtg ctccattcca ctcgtcgacg cgtgagtttt agctgtcgcg cgcaccacaa
      781 gaactcttac tacaagattc caatgactgg cgggcttaaa attttctgcc acgctgtcgc
      841 taccgtctag ttccttgccc gccttccact gcacgaaccc accaaaaatg gaccgagcag
      901 ctgaccccga gcctcttctc gctatttctg aaatctttga agcgggaatg tctaatccag
      961 cagctcgtac tgctgccaaa gctaccgccg aaaggcctga agccgaagat gcgagtcccg
     1021 ctgcagtcgg aaaattattt cgactttgaa cccgcgcttt atgcgacttt ccagaaagtt
     1081 ttcgtacctc gtttaatact gcaataactt tggtaatttt ttccgacgat tctatagaac
     1141 catttaaaat taattcgtcg t
//
LOCUS       DAOMXO010000742         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_72359_length_1160_cov_4.276923, whole genome shotgun sequence.
ACCESSION   DAOMXO010000742 DAOMXO010000000
VERSION     DAOMXO010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72359_length_1160_cov_4.276923"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..531
                     /locus_tag="PLH57_12045"
     CDS             79..531
                     /locus_tag="PLH57_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03384.1"
                     /translation="MTRFERAIYNFLNARGREIMKCLIATLCADDPAIISRVRIQRVI
                     LRTEKAIAKMPLFYFVVLKIGVYLLEYAIPPLSWKIRPFTSMNLDQRLEYLNTWSSSR
                     FYYKRMILKLLISLCLPRLYSEKRLLLSIGFERALQGRTRKRWSTAPL"
     gene            510..>1160
                     /locus_tag="PLH57_12050"
     CDS             510..>1160
                     /locus_tag="PLH57_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRK03385.1"
                     /translation="MVDGSTLREHIRENFDVVIVGSGAGGAVVAKELAEGGMRVALVE
                     EGPAFAPQDHRDMAVDAIYRMYRDRGFTTTFGKPAIPIPMGRVLGGTTAINSGTCFRT
                     PSKIFRHWNEELGLHELNEAEFQPIFDRVEREINVEPSDFAVMSRANTIFHELLQNRG
                     TPGKPLRRNIRKCDGCGYCCYGCATGAKQSTDVSYVPKAIRAGATVYTNCTVEKFLI"
BASE COUNT          294 a          274 c          282 g          310 t
ORIGIN      
        1 agaaacgctt aaatcttccg gggctgctgc tttcgaaacg gtgtcccgca ataaaaatcc
       61 ctatgtagag gttttgctgt gacgcgtttt gagcgagcaa tttacaattt tctaaacgct
      121 cgcggtcgcg aaatcatgaa gtgccttatc gctactttgt gtgctgatga cccggccatt
      181 atttcaagag tacgaattca gcgagtaatt ctacgcaccg aaaaagcaat cgcgaaaatg
      241 ccgttgtttt atttcgtcgt tttaaaaatt ggggtttatc tccttgagta cgcgattcct
      301 ccactatcgt ggaagattcg accgtttacc tctatgaatc ttgatcaaag gctggaatat
      361 ctaaacacct ggtcgtcgag ccgtttttat tacaaacgca tgattttaaa acttttgatt
      421 tcgctgtgcc tgccgcgcct ttattcggaa aaacgtttac tgttaagcat cggctttgag
      481 cgcgcacttc aaggtcgaac gaggaaacga tggtcgacgg ctccactcta agagaacata
      541 tccgcgaaaa tttcgatgtc gtaatagtgg gcagcggtgc cggcggtgcg gtggtggcaa
      601 aggagctcgc agaaggtgga atgcgagtcg cactagttga agaaggaccc gcttttgcac
      661 cgcaagatca ccgcgatatg gccgtcgatg cgatttaccg catgtatcgc gatcgcggtt
      721 tcactactac ttttggaaaa cccgctatcc caattccgat ggggcgcgta cttggtggca
      781 ctaccgcgat taattccggc acttgttttc gcactccttc aaagattttt cggcactgga
      841 atgaagagct tggtctacac gaattaaatg aggcggagtt tcagccgata ttcgaccgcg
      901 ttgaacgcga aatcaacgtt gaaccatctg atttcgccgt catgagccgc gcaaatacga
      961 tctttcacga actactccag aaccgcggaa caccaggtaa acctctacga cgtaatattc
     1021 gcaaatgcga cggctgcggt tattgttgtt acggctgtgc gaccggtgca aaacaaagca
     1081 cggacgtaag ttacgttccc aaagctattc gagctggtgc gacggtttac accaattgca
     1141 cggtcgaaaa atttttgatt
//
LOCUS       DAOMXO010000743         1155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_72801_length_1155_cov_4.824545, whole genome shotgun sequence.
ACCESSION   DAOMXO010000743 DAOMXO010000000
VERSION     DAOMXO010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72801_length_1155_cov_4.824545"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PLH57_12055"
     CDS             <1..860
                     /locus_tag="PLH57_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03386.1"
                     /translation="CSKKIFHSHREPLSSVSRTYYISKMNVQAQRTAFFLFVSLTVSL
                     PFTLLATTFVDRPMTAVVNEAQAIVRGVVVGTHVDYEPSGSGRIMTYTRVKIDEVLKG
                     TISEKEVLVRQPGGAKDGMEMRVAGTAEFKEGEDVVLLLGPFDSRDQSYFLPGLATAK
                     FNVERRKNGDEVLVNSLGSDEIYQKSKNQGELSYTSRVVLSEFRTMAAASHSANEPPS
                     PPKTESPGATPASTSHNDSETETTDTPTTALNEEGTKTPEPKKTNGFIITIIALCVMG
                     AAWFWTRRR"
     gene            900..>1155
                     /locus_tag="PLH57_12060"
     CDS             900..>1155
                     /locus_tag="PLH57_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03387.1"
                     /translation="MKHTLALILGIASTLAIAAPPLKQSFMTDSAGKKKNHYVSDGTV
                     IGGNQLVRTFRITDLRRSPIGKDGYERVVFELDSGEATKEP"
BASE COUNT          327 a          306 c          263 g          259 t
ORIGIN      
        1 cttgttctaa aaaaatattt cattctcacc gagaacctct ttcttctgtt tctcgaacct
       61 attacatttc taagatgaac gtacaggcac agcgcacggc atttttctta tttgtgagtc
      121 taacggtttc cttgcccttc acattacttg ctaccacgtt tgtcgatcgg cccatgaccg
      181 ccgtagtgaa cgaagcgcaa gcgatagtgc gcggagtggt ggttggaacc catgtcgact
      241 acgaaccaag cggttcgggc cgcatcatga cctacacccg cgtaaaaatc gacgaagtgc
      301 ttaaaggaac catcagtgaa aaagaagtgc tcgttcggca gccaggcggc gccaaagacg
      361 gcatggaaat gcgggtcgcc ggaaccgccg agttcaaaga aggcgaagac gtggttttac
      421 tactggggcc ttttgattca agggatcaaa gttatttttt acccggattg gcgactgcca
      481 agttcaatgt cgaacgtcga aaaaacggcg acgaagtact cgtcaacagt ctgggttcgg
      541 acgaaatata tcagaaatcc aaaaaccaag gtgaactctc gtacacctcg cgcgtggtcc
      601 tttcagaatt tcgaaccatg gctgctgcta gtcattcagc caacgaacca ccatctcccc
      661 ctaaaaccga atcacccgga gctactcccg cgtcgacttc ccataatgac agtgaaactg
      721 aaactaccga cacaccaact acggcactaa acgaggaagg caccaagact ccagaaccga
      781 aaaaaaccaa cgggttcatt atcacaataa ttgctctatg cgtcatgggg gctgcgtggt
      841 tctggactcg tcgccgctaa gattgccgcc ttcaatattt caagctaaac tgacccagta
      901 tgaaacacac actcgcactg attttaggaa tcgcctcaac tttggcaatc gcagcaccac
      961 ctcttaaaca aagttttatg accgactctg ctggaaagaa aaaaaatcac tacgtctccg
     1021 atggtacggt tatcggtggt aatcagttgg tgcgcacgtt cagaatcacc gaccttcgaa
     1081 gatcgccaat cggcaaagat ggttacgagc gcgtggtgtt cgaactcgac agcggcgaag
     1141 caaccaaaga accgc
//
LOCUS       DAOMXO010000744         1151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_73172_length_1151_cov_3.827555, whole genome shotgun sequence.
ACCESSION   DAOMXO010000744 DAOMXO010000000
VERSION     DAOMXO010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73172_length_1151_cov_3.827555"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PLH57_12065"
     CDS             complement(<1..1109)
                     /locus_tag="PLH57_12065"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRK03388.1"
                     /translation="MVPTHFSNLDSVLLGWALFEMGLPPFCFGAGLNLFSNPLLGYFM
                     NHLGAYKIDRRKKHSLYRWTLMNYSSVALENDCHSLFFPGGGRSRSGAPEFVEGNLKP
                     GLLSTVLRAYIRNIQKKKTNPNLYIVPCVISYHVVLEATSLIEEHLKRTGANRFMIDN
                     DESFAPKTIAKFIYNFMRGSSSVNLHFCRPLDPFGNLVSEDGLSRNQAGRILNLPDYI
                     TTDGEIGSKPQRDREYGLNLNQKILDRYKQENVVLSSHFIAYVFFEYLLQRFGEGDPL
                     RLFKHQTEQCKLTTDELRTYAVPILKKLQILHEGGGVLLEPVLRSNDFDQVLESAGRN
                     LAVFHTARPILVEGQNVGTEDLRLLYYYHNRLEGYG"
BASE COUNT          320 a          260 c          252 g          319 t
ORIGIN      
        1 ccgtagccct ccaaacgatt atggtaataa taaagcagcc gcaaatcttc cgttcctaca
       61 ttttgacctt caactaatat aggtcgcgcg gtatggaaaa ccgccaaatt tctacccgcc
      121 gactcgagta cctggtcgaa gtcgtttgag cggagaacag gctctaatag aactccgccc
      181 ccttcgtgta atatttgtaa tttttttaaa attggaacag cgtacgttcg aagctcatca
      241 gtcgttaact tgcattgttc agtttggtgt ttgaagagtc gcagtggatc gccttcgccg
      301 aatcgttgca gcaaatattc aaaaaaaaca taagcaataa aatggctgct taaaaccaca
      361 ttctcttgtt tatagcgatc tagaattttt tgatttaaat tcagcccata ttcccgatct
      421 cgctgcggct tcgagccgat ctccccgtca gttgtgatgt agtcgggcaa gtttagaatt
      481 cttcccgcct gattgcgact tagtccatct tcggaaacca agtttccgaa cgggtccaaa
      541 ggtcgacaaa aatgtagatt tacagaagag ctgcctctca tgaaattata aataaacttc
      601 gcgatagttt ttggtgcaaa tgattcatcg ttatcgatca tgaaacgatt ggcaccggtg
      661 cgttttaaat gttcctctat cagcgatgtg gcctcgagca ccacatggta ggaaatgacg
      721 cacggaacga tgtacaaatt cgggttggtc ttttttttct gaatatttcg aatgtaggcc
      781 cgtaatacgg tactgagtaa tcccggcttg agattgcctt ctacaaattc cggggcgccg
      841 gatcgacttc gtccgccacc aggaaaaaat aaagaatggc aatcgttctc cagagcaaca
      901 ctcgaataat tcataagggt ccaccgatag agcgagtgtt tcttgcgccg atcgatcttg
      961 tacgccccca ggtgattcat aaaatagccc agaagcggat ttgaaaataa gttgagcccc
     1021 gccccaaaac aaaacggcgg aagtcccatt tcgaataaag cccaaccgag aagtacggag
     1081 tctagattgc tgaagtgtgt aggtaccagt acgattgtgc cttttttgga taggtttttg
     1141 attaactgaa t
//
LOCUS       DAOMXO010000745         1148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_73439_length_1148_cov_4.133577, whole genome shotgun sequence.
ACCESSION   DAOMXO010000745 DAOMXO010000000
VERSION     DAOMXO010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73439_length_1148_cov_4.133577"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..>1148)
                     /locus_tag="PLH57_12070"
     CDS             complement(59..>1148)
                     /locus_tag="PLH57_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03389.1"
                     /translation="TQHELAERGWDHLFVRGAAPLVRHLNQKFPKLFQLASIQTWAQD
                     SQNGLFAGPSVKSTAIEVIRKDDTLGFLPLNRNPEPGKTLIFPHNPSDVAWLVAMLNS
                     SPVQFWLKHQLFVQGAGKTPRLQELKSAPMPDLSSVPSELVHEALDWIGEKKPAAAAL
                     KTWAFGGANGDSHDAKQARFVAMAKKAHTLRRAAERYRPLFNVNATKECFALLPESVT
                     QFYPSLLLARLRDCPEIRVHFADRSKTPLSPDHWILADVSAVSQGDLAFVLINARQGP
                     VIQLTVPIALKNYVVAQLKELKDHLWSEATHLLRIPRDIPLFTAQTTEIVRVIGDIDS
                     EIAIYEFVLNLLSQDLFEVAGDMKEFLH"
BASE COUNT          313 a          271 c          295 g          269 t
ORIGIN      
        1 gctccaaaaa aaacgtagtg tgaaggacct accagactag gcataacggt ttatagtctc
       61 aatgaagaaa ctctttcatg tccccggcga cttcaaacaa atcctgcgag agtaaattca
      121 atacgaactc ataaatggca atttcagagt caatatcccc aatcacgcga acaatttcgg
      181 tggtttgggc cgtaaagagc ggaatatcac gcggaatccg aagtaaatgc gtggcctcgg
      241 accatagatg atcttttagc tctttgagtt gcgcgacaac gtagtttttg agcgcaattg
      301 gaaccgtgag ttgaatcacg ggaccttgtc gggcattaat gagtacgaac gccaagtcgc
      361 cctgcgaaac tgcactgacg tcggctaata tccaatggtc cggtgaaagc ggtgttttgc
      421 tgcgatcggc aaaatgcact cgaatttccg gacagtcacg caaacgcgct aacaacaatg
      481 aagggtagaa ctgggtaacc gactctggaa gcaaagcaaa acactctttg gtggcgttca
      541 cattgaacag agggcgataa cgttcggcgg ctcgcctcaa agtatgcgcc tttttggcca
      601 tagcgacaaa gcgagcttgc ttagcatcat gcgagtcgcc gttagcgccg ccaaaagccc
      661 aagtctttag cgcggcggcg gctggttttt tttcgccaat ccaatcgagt gcttcgtgca
      721 ccaactcact tggtaccgac gaaagatcag gcatcggagc cgatttgagt tcctgcaaac
      781 gtggagtctt acccgcaccc tgaacaaaga gttgatgttt gagccaaaac tgaaccggtg
      841 aagaattgag catagccact agccaggcaa catctgaagg gttgtgcgga aaaattaagg
      901 tttttcctgg ttctgggttt cgattgagcg ggagaaatcc gagcgtgtcg tcttttcgaa
      961 tcacttcaat cgcggtgctt ttaaccgacg ggccagcaaa aaggccattt tgactatctt
     1021 gagcccaagt ttgaatcgac gctaactgaa aaagtttcgg aaatttctgg ttcaaatggc
     1081 gcacaagtgg tgcggcacct cgaacgaaaa ggtggtccca accgcgctca gcaagttcgt
     1141 gttgagtg
//
LOCUS       DAOMXO010000746         1146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_73604_length_1146_cov_7.197984, whole genome shotgun sequence.
ACCESSION   DAOMXO010000746 DAOMXO010000000
VERSION     DAOMXO010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73604_length_1146_cov_7.197984"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(459..782)
                     /locus_tag="PLH57_12075"
     CDS             complement(459..782)
                     /locus_tag="PLH57_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03390.1"
                     /translation="MSTTSLEQDLHNRLPKAPDLIYCTARGHWPVPSAAQLTPTSNRL
                     KPTDDFFGKRSLEKRFNGKVRHRTAPLERCNAALRRKIRTVRRCYCTDLCSNSTETHP
                     NCTVR"
BASE COUNT          253 a          272 c          331 g          290 t
ORIGIN      
        1 gtgaacgagt ttttcaggtg gtcaacgaca aatgcgtagc tgcggcagcc ctgggtacgg
       61 gaccacgttt aagttagttg caataaccca atgttcacaa cggaaaacac cctcaggaag
      121 tcaccgaacg gtgcacgtga ggtgatttcc ggtgttgtta ctgcaccgaa cggtgcgaat
      181 ttgtcgacgg cgtgcagcgt tacattccca gattccgata gccggcactg actgtcctcc
      241 cgaactgtgg tggtacccga gcgggtaaac ggcgggctaa agatctgatg ctcgatgagg
      301 gcgccaaaga atcgccggcg gcaacaggaa ggccttcgct ggtagcccaa agagcgttaa
      361 gaaaagaagc tttttcaact aacttaaaca cactacggga ccatttttaa gttagttgca
      421 atagcccaat gtttacaatg ggaaacaccc caaggaagtc accgaacggt gcaattcggg
      481 tgagtttcgg tgctgttact gcacaggtct gtgcagtaac agcgacgtac ggtgcgaatt
      541 tttcgtcgga gcgcagcgtt acatcgttcg agtggagccg tgcgatggcg aacttttccg
      601 ttgaagcgct tttcaaggct gcgtttacca aaaaagtcgt ccgttggctt caagcggttg
      661 gaggtgggcg tcaattgcgc cgccgagggc acaggccaat gtccgcgcgc cgtgcagtag
      721 atgaggtcgg gcgcctttgg taatcggtta tggagatcct gttcgaggct ggttgtgctc
      781 atgcgatttc tcggcgtccc tgttctttcg ggcatctttg gatgcgggat cgccaacttg
      841 ttttccgtgg atcactgtgc ttgagggctg ggcgcctatt gtgtgagatc aggtcttcca
      901 gcgttcttgt gggtgtcctc ggacccggtc gatagggtgt tacacacccg atttcatgag
      961 cgcgtccgtt acaagtagtt cggaccttct tcgatttata tagcaacgtg gttttgcgcg
     1021 tgattggtgc catgatcaga taactaattg aatacatgta tgtaacgtct cgatgtggcc
     1081 aggttccgag gcagggcaat cgcaccgact tcgcataagc caatgcaata actcgttaac
     1141 tttcat
//
LOCUS       DAOMXO010000747         1143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_73879_length_1143_cov_7.887868, whole genome shotgun sequence.
ACCESSION   DAOMXO010000747 DAOMXO010000000
VERSION     DAOMXO010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73879_length_1143_cov_7.887868"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1143
                     /locus_tag="PLH57_12080"
     CDS             <1..>1143
                     /locus_tag="PLH57_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03391.1"
                     /translation="IGAARAGAAIEARASLFNRASTVAAADGSFSVTLNLFSNVTITA
                     TDSSIGRSDSADFSYGINTSLSGIVKDTNDQPLANVTVSISGSNLFSVTDGSGVFTFT
                     EPVTGDQILVVDGTTVPLLPPPAPQRKFAKTSIALSIGLAQANVLTRPIYLTPIPLDG
                     SATELNAAQGAVVTSPHAPGVEIDIPAGVIEFPADEASGLISIATIPASRSTVAPPDF
                     AEPLEVVSLEPSGVKFKEPVNITLPNVNELPPGVNVVIMSMNSTEGRWEIDGIGEVSS
                     DGNSIVTEDGNGITHFSTIYATPVGPKVSTATKQDRPGANTFDGSLSSSITLPSYKAL
                     GKSIAPSLIYKSSWANPQVLVSNIFDIPTGRISFGTEDRINGSEFY"
BASE COUNT          291 a          309 c          259 g          284 t
ORIGIN      
        1 caatcggtgc cgctcgtgcg ggggctgcaa ttgaagcgcg ggctagttta tttaatcggg
       61 ccagcacggt cgccgctgcc gatggcagct ttagcgtaac gctaaatctt ttttcaaacg
      121 tcacgatcac ggcgactgat tcaagcattg gtcgttctga ttccgctgat tttagctacg
      181 gcattaacac ttcgctctca ggcattgtaa aagacaccaa cgatcagccg ctcgctaacg
      241 ttacggtgag catttcaggg tcaaatcttt ttagcgtaac cgatggcagc ggggtcttta
      301 cttttactga gccggttact ggtgaccaga ttttggtggt cgatggaacc accgtgccac
      361 tcttaccacc gccagcaccc caacgaaaat tcgcaaaaac ctcgattgcg cttagcattg
      421 gcctggctca agccaatgtt ttgactcgtc cgatttattt aactccaatt ccactcgatg
      481 gttcagctac tgagcttaac gctgcacaag gcgcagtggt gaccagccct cacgcgcccg
      541 gcgttgaaat cgacattcca gccggcgtca tagaatttcc agccgatgaa gcaagcgggt
      601 taattagtat tgccaccatt ccagcttcgc gctcaaccgt agctccgccc gatttcgctg
      661 aaccactcga agtcgtttct cttgaaccaa gtggcgtaaa attcaaagaa ccagtcaata
      721 taacgctacc aaacgtaaat gaactaccgc ccggcgtgaa tgttgtgatc atgagcatga
      781 actcgacaga aggtagatgg gaaatcgatg gcattggtga agtcagttcc gacggcaatt
      841 cgattgtcac cgaagatggc aacggcatca ctcacttttc aaccatttac gccacaccag
      901 tcggacccaa agtttcaacc gcaactaaac aagatcgccc aggcgccaac acctttgatg
      961 gttcgttatc gagtagcatt acgctgccaa gctacaaagc gttaggcaaa agcattgctc
     1021 cctcactcat ttacaaaagt tcttgggcta atccccaagt acttgtttca aatattttcg
     1081 atatcccaac cgggcgcatc agctttggta ccgaagatcg catcaatggc agcgagtttt
     1141 att
//
LOCUS       DAOMXO010000748         1142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_74006_length_1142_cov_4.256670, whole genome shotgun sequence.
ACCESSION   DAOMXO010000748 DAOMXO010000000
VERSION     DAOMXO010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74006_length_1142_cov_4.256670"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLH57_12085"
     CDS             <1..484
                     /locus_tag="PLH57_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRK03392.1"
                     /translation="TDDSFLFNQGATKGLGKIQFHDYRTAFESVDLPSVKTFRRQIAA
                     YKRLLFLAGPSKDQIKDFDFLLIFGELFTLVAYGQLIIEQCQKSRIENDLLDQIFDFM
                     VRDFSHFALRLYAKPSTTRLQAMLAQTMIRKPSANRSSFLNVWQKHVHSLRENYEMKP
                     "
     gene            558..830
                     /locus_tag="PLH57_12090"
     CDS             558..830
                     /locus_tag="PLH57_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK03393.1"
                     /translation="MMRRDNQPKTDHEIDIEFGQWLRDRRTRSGLTLEQAAERSKMPV
                     ERLKSLELGYAQRGVTQQESEKICATFKINLQEFLDRAAGKELNHS"
     gene            874..>1142
                     /locus_tag="PLH57_12095"
     CDS             874..>1142
                     /locus_tag="PLH57_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRK03394.1"
                     /translation="MAVAVIDGARTPFTKMGTSLRNSTAVDLGVGPVNTLVSRYNLSK
                     DPGGLLVFGSVILHSDVSNLAREVALESTLHPETRAFTSIMACAT"
BASE COUNT          313 a          284 c          258 g          287 t
ORIGIN      
        1 cacggacgat agcttcctct tcaaccaggg cgccaccaag gggctgggga aaatccagtt
       61 tcacgactac cgaacagcct tcgagagtgt cgacctaccc agcgttaaaa cctttcgacg
      121 ccagattgca gcctacaaac ggttactttt tctcgccggc ccatctaaag atcagatcaa
      181 agattttgat tttttactta tctttggcga gctctttact ttggtggcct atgggcaact
      241 catcatcgaa cagtgccaaa aatcgcgaat agagaacgac cttttagatc agatatttga
      301 ctttatggtg cgggattttt cgcattttgc actgcgatta tacgcaaaac cgagtacgac
      361 gcgacttcag gcgatgctcg cccaaacgat gattcgaaaa ccttcagcta atcgttcgag
      421 ctttttaaac gtttggcaaa aacacgttca ttcgttacgc gaaaattacg aaatgaagcc
      481 gtaaaaatat gaaaaaaagc tcaacatccc caaagttctt ctagacaatg aggcctccat
      541 tctagaaagt agggcccatg atgagaagag ataaccaacc taaaaccgat cacgaaattg
      601 atattgaatt tggccaatgg ctacgcgatc gcagaacgcg aagcgggctt acgcttgaac
      661 aagccgctga acgttcgaaa atgcctgttg agcgtttgaa gtctcttgaa ctgggctacg
      721 cacaacgcgg tgttactcag caagaatccg agaagatctg cgctaccttc aaaatcaatc
      781 ttcaggaatt tctcgatcgt gccgctggca aagagctgaa ccactcataa cgctgtatca
      841 ggcattgtct gatccgggct cgaaggggga tttgtggcag ttgctgtgat tgacggtgca
      901 agaacaccgt ttaccaaaat gggtaccagt ctccgcaatt cgactgcggt tgatctcggt
      961 gtcggacccg taaacactct agtatctcga tacaatcttt cgaaagaccc tggtggcctt
     1021 ctggtttttg ggtcagtcat acttcactct gatgtaagca acctcgcgcg tgaagtggcg
     1081 ctcgagagca cattacaccc cgaaacccgc gcttttacct ctattatggc ttgtgcaacc
     1141 ag
//
LOCUS       DAOMXO010000749         1142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_74036_length_1142_cov_3.007360, whole genome shotgun sequence.
ACCESSION   DAOMXO010000749 DAOMXO010000000
VERSION     DAOMXO010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74036_length_1142_cov_3.007360"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1142)
                     /locus_tag="PLH57_12100"
     CDS             complement(<1..>1142)
                     /locus_tag="PLH57_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRK03395.1"
                     /translation="NVDKITDFLFRTKKEGAQLAVFSELTLCSYAPLDLVERSDFLEA
                     HDAALLRLRDQLTGDDVPEIVVIGGLERNKKLSGRALHNSAFVFQKGELLDSYAKRLL
                     PTYDVFDEARYFEPGDRTCKLDIKLTGGESLRLGISICEDAWFEQEASGRRRYTDDPA
                     HDLRGCDLVVNLSASPFELNKRERRQSEISGFALRVGAPVVYVNQVGANDEILFDGAS
                     LVFDATGRKIFEAPVWDESLDLVELNVEKKEIVAPKISSTKKVNSSDPMEVLFDGLVV
                     GIHDYFSKSGFSKAVIGLSGGIDSAVVAVLAAAALGPKNVLGVSLPGPYSSSHSLADA
                     EELANKLGIEHRVHSIKFMNTAALMELKSSFAGLPPDVTEENLQSR"
BASE COUNT          312 a          296 c          255 g          279 t
ORIGIN      
        1 agcgcgactg gaggttttct tcagttacgt cgggcggcag gccggcgaaa ctggacttta
       61 gttccatgag cgcagcggtg ttcataaact taatggaatg aacgcgatgt tcaattccta
      121 gtttgttagc aagttcttct gcatcggcca gactatgact cgaagaatag ggcccgggca
      181 gcgaaacccc taagacgttt ttggggccca acgccgccgc tgccagcacc gcaactaccg
      241 cagaatcaat tccaccagaa aggccaatta ctgctttaga aaatcccgac ttcgaaaaat
      301 aatcatgaat acctaccacc aacccgtcga aaagaacttc cattgggtca ctggaattca
      361 cctttttggt agacgaaatt ttgggagcga cgatctcttt tttttcaaca ttaagttcca
      421 ccaaatctag cgactcatcc cagactgggg cttcgaaaat ttttctaccg gtcgcgtcga
      481 aaaccaaact cgcaccgtcg aacaaaattt catcgttcgc gccaacttga ttgacgtaaa
      541 ccacgggcgc gccaactctc aacgcaaatc ccgaaatttc agattggcgt cgctctcgtt
      601 tattcagttc gaagggacta gcgcttagat tcaccaccaa gtcacagcca cgaagatcat
      661 gagcgggatc gtcagtataa cggcgtcgac cagaggcttc ttgttcaaac cacgcgtctt
      721 cgcaaatcga aatacctaaa cgaagtgatt cgccacctgt tagcttaata tccaacttgc
      781 aagtgcgatc gcctggttcg aaatatcggg cttcatcgaa gacgtcgtag gtgggcaaaa
      841 gtcgtttggc ataactatcc aacaactccc ctttttgaaa aacgaacgca ctattatgaa
      901 gcgcgcggcc agagagtttt ttgtttcgtt cgaggccccc gatcacaacg atttcgggaa
      961 cgtcgtcacc agttagttgg tcgcgtaatc gcaaaagtgc ggcatcatga gcctctagaa
     1021 agtcacttct ttcgactaga tccagaggcg cgtaagaaca aagtgttagc tccgaaaaaa
     1081 ccgcaagttg cgcgccttct tttttggtgc gaaacaaaaa atcggtgatt ttatccacat
     1141 tg
//
LOCUS       DAOMXO010000750         1140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_74190_length_1140_cov_4.170507, whole genome shotgun sequence.
ACCESSION   DAOMXO010000750 DAOMXO010000000
VERSION     DAOMXO010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74190_length_1140_cov_4.170507"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PLH57_12105"
     CDS             complement(<1..166)
                     /locus_tag="PLH57_12105"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRK03396.1"
                     /translation="MDKLQFKVGDNAVYPAHGVAVIKKIDEKEVGGKKKRFYVLQVLE
                     NEMTIMVPTDN"
     gene            complement(359..>1140)
                     /locus_tag="PLH57_12110"
     CDS             complement(359..>1140)
                     /locus_tag="PLH57_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03397.1"
                     /translation="FIGRTIRGTISVKRIYAVLTLLTLMLASPWVFAQDDHSEPCAPV
                     LRNVQTDGVRQLLAGDPTKRYIVYLVVPYYGQNPLNPEYPRWPQIEEELRYFDLETLA
                     VLESYSTEGRRLVKVNGMGQKLQEASHSPGVLWMGLKPNRRAVHSTLKKGIPLIPERK
                     PPSHNVVVDPAVVDFFSKNKQGLLRVNLTPREVSGGAGNTLEGVVRQFNENHGSRAWH
                     LELVRPARGKEPAIFEINLKTYELCLEFPHLVEKIASALHL"
BASE COUNT          290 a          299 c          259 g          292 t
ORIGIN      
        1 tgttgtcagt tggaaccatg atggtcatct cgttttcgag tacctgtaaa acatagaatc
       61 gcttcttttt gccaccgact tctttttcgt cgattttctt gatgacagca actccgtgcg
      121 caggatagac cgcgttgtcg ccaactttga actgcaattt gtccatgaat acaatcctta
      181 ctagacccta aaccgcgggg tcgcagcatt gttaaaaacc ttgctcgaga cgacctaaaa
      241 tacgcctcaa gtggccccgg aatttaacac agcactcgga ttttgtaaag acagactgag
      301 tttgaccaca atcgaaatta aatcaataat tacagcatat taccggacca gctgagactt
      361 acaggtgcaa tgcactcgcg attttctcta ctaaatgcgg gaattccaaa caaagttcgt
      421 aggtttttaa gtttatttca aatattgccg gctcttttcc acgagctggg cgaaccaatt
      481 ccagatgcca tgccctcgac ccatgatttt cgttaaactg tcgcacgacc ccctcgaggg
      541 tgttccctgc accgccactc acctcacgcg gggttaggtt cactcggagg aggccctgtt
      601 tatttttaga aaaaaagtca accaccgctg ggtccaccac cacgttgtgg gacggtggtt
      661 ttctctcggg aatcagcgga ataccctttt ttaaggtgct atgaacggct cttcgattag
      721 gtttaagccc catccagagc acacccggtg aatgcgacgc ctcttggagc ttttgcccca
      781 tgccgttaac ctttaccaac cgacgccctt cagtggagta ggactcaagg accgccaggg
      841 tttcaagatc aaagtaacga agctcctctt cgatctgcgg ccagcgtggg tattcagggt
      901 tgagagggtt ttgcccgtag taaggcacta ccaaatacac aatgtatcgc ttggttggat
      961 cacccgcgag caactgccga accccatcgg tctgaacatt gcgaagaaca ggcgcgcaag
     1021 gttccgaatg gtcgtcttgc gcaaaaaccc aagggctggc cagcattaaa gtcagcagtg
     1081 tcaaaacggc gtaaatgcgt ttcacggaaa ttgtccccct aatggttctg ccaataaaat
//
LOCUS       DAOMXO010000751         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_74431_length_1137_cov_5.462107, whole genome shotgun sequence.
ACCESSION   DAOMXO010000751 DAOMXO010000000
VERSION     DAOMXO010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74431_length_1137_cov_5.462107"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLH57_12115"
     CDS             <1..255
                     /locus_tag="PLH57_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRK03398.1"
                     /translation="SRMTVLAALESVGYVTWFSGGNPIPTIKAINPKVLVKGGDWKID
                     QILGSKEVLRSGGKVYSLPFVAGKSTTRVIEKIRRSKCKS"
     gene            243..>1137
                     /gene="meaB"
                     /locus_tag="PLH57_12120"
     CDS             243..>1137
                     /gene="meaB"
                     /locus_tag="PLH57_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRK03399.1"
                     /translation="MQIVKKMLAGDRLALARVISVLENRGDALEKLVKNLYPKTGNAF
                     VLGITGPPGAGKSTVTDRLIARFRAAKLKTAVIAIDPSSPFSGGAILGDRIRMQSHTG
                     DKGVFIRSLGTRGRHGGLSLSSREVVMALDAAGFDVIIVETAGVGQTELDILKLAHGV
                     CVVLVPESGDGIQVMKAGLMEIADIFVVNKCDRPESDKLVRELTNMLHMFQKGTDWEI
                     PVIKTQATQNQGIEELFQSVLSYQKHLKNTDKVDKKRLEFLREELIEIVRNAYGHKMN
                     EALDTKKGQKALTDLFLKKKSP"
BASE COUNT          320 a          237 c          306 g          274 t
ORIGIN      
        1 tcgcgaatga ccgtcttggc ggcacttgag agtgttggat acgtgacctg gttttcaggc
       61 gggaatccaa ttcctacgat caaagcgatt aaccctaaag ttctggttaa aggcggtgac
      121 tggaaaatcg atcaaatctt aggttcgaaa gaagttttac gtagcggtgg caaggtctat
      181 tcgctgccgt tcgtagccgg caaatccact acacgtgtga tcgaaaaaat tcgtaggtcc
      241 aaatgcaaat cgtaaaaaaa atgctcgcgg gagaccgtct tgcgttggct cgcgtaattt
      301 ctgttttgga aaatcgtggt gacgctctcg agaagttggt gaagaacctc taccccaaga
      361 ccggaaatgc gtttgtactg ggaataactg ggcctccggg ggcgggaaag agtactgtga
      421 ccgatcgctt gatcgccagg tttcgggccg cgaaacttaa aaccgctgtg atcgcgattg
      481 atccatctag cccgttcagt ggtggtgcga ttttgggtga tcgcattcga atgcaatcgc
      541 acacgggcga caaaggtgta tttatacgaa gtctaggcac gagaggccgt cacggtggat
      601 tatcgctttc gtcccgcgaa gtggtcatgg cgctcgatgc ggccggattc gatgtgatca
      661 ttgtagagac tgccggagtt ggtcagactg aactcgacat tttgaagctt gcccacggcg
      721 tatgcgtggt attagtgcca gaaagcggcg acggcatcca agtaatgaaa gccggattaa
      781 tggaaatcgc cgacattttt gtagtaaata agtgcgacag acctgaatct gacaagctgg
      841 tgcgagaact gacgaacatg ttgcatatgt tccagaaggg aactgattgg gagattccgg
      901 taatcaaaac ccaagcaacc caaaaccagg gaatcgaaga gctgtttcaa tcggtacttt
      961 cttatcaaaa acatctaaaa aacactgata aggttgataa aaagcgtctg gaatttctgc
     1021 gagaagagct gatcgagatc gttcgtaacg catatggtca taaaatgaac gaggcgctcg
     1081 atactaaaaa aggccagaag gccctaactg atttattttt aaagaaaaaa agtcctt
//
LOCUS       DAOMXO010000752         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_74432_length_1137_cov_5.430684, whole genome shotgun sequence.
ACCESSION   DAOMXO010000752 DAOMXO010000000
VERSION     DAOMXO010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74432_length_1137_cov_5.430684"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1137)
                     /locus_tag="PLH57_12125"
     CDS             complement(<1..>1137)
                     /locus_tag="PLH57_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03400.1"
                     /translation="DLTNAKTKYPGLQPFELLVSESQERMSFAVPRSKIEEFLALASR
                     RGVECSVLGTFEDNGIFEVSYRSQVVAKIDLHFLHEGCPRMNLNARVPKVSLAIGSAR
                     VGAWQSLMQERTPLKMVEALPQILASENVRSRRWISEQYDHEVGARSVRAPYKTKERG
                     SPGDGGALRISRNTGLGVAVGIGLAPKIAAHDPACAAAVAVDEAFRNALVAGLDPDHA
                     ALVDNFCWPDPLPSGSNPDAEIKLGALVSTNQILHDAAIALGAPFVSGKDSMKNDYRM
                     GSLKISVLPTILVTAIGKIHDVRQLPRGFCAGSNRSVYWIAASDPAPDGRGLPRVDFS
                     RLSRFARAFHQAHREGWLESAHDVGDGGWLVTTAFILWRSAPDAM"
BASE COUNT          264 a          342 c          289 g          242 t
ORIGIN      
        1 catggcgtcc ggggccgacc tccagagtat gaatgcggtg gtgacgagcc acccgccatc
       61 gcccacgtca tgcgcgctct ccaaccaacc ctcgcggtga gcctgatgaa aagctctggc
      121 aaagcggctt aaccgactaa agtctacccg cggtaaccca cgaccatcgg gggccgggtc
      181 agatgccgcg atccagtaaa ccgagcgatt gctgccagcg caaaacccgc gcggcaactg
      241 tcgtacgtca tgtatttttc cgattgcggt tacaagaatc gtcggaagta cgctgatctt
      301 caacgaaccc atgcggtagt cgtttttcat cgaatctttg cccgatacaa aaggagctcc
      361 caaggcgatg gctgcgtcat gtaaaatttg gttggtactc acgagtgcgc ccaattttat
      421 ttcggcgtcg ggattactcc cgctcggcaa aggatccggc caacaaaaat tatcgaccaa
      481 cgccgcgtga tcggggtcaa gaccagcaac aagggcattg cggaaggctt catctactgc
      541 aaccgcggcc gcgcacgctg ggtcatgggc ggcaatcttg ggcgctagtc caatgcctac
      601 ggccacgccc aaacctgtgt ttcgtgaaat gcgcaaggca ccgccatctc ccggggagcc
      661 gcgttctttc gttttgtagg gcgctcgcac cgaccgcgcg cccacttcgt gatcatactg
      721 ttccgaaatc cagcggcgac ttcgcacgtt ttccgaagct aaaatctgag gtagggcttc
      781 aaccattttt aacggagttc gttcctgcat caacgactgc caggccccga cacgagcact
      841 tccgatcgca aggctaacct tcggcactcg cgcatttaga ttcatgcgcg ggcagccttc
      901 gtgtaaaaag tgaaggtcta ttttcgccac cacctgcgaa cggtagctca cctcaaaaat
      961 gccgttgtcc tcgaacgttc ccaacaccga gcattccaca ccacgtctac tggctaatgc
     1021 caaaaactct tcaatctttg atcgcggaac agcaaacgac atgcgttcct gagattcact
     1081 aacgagaagc tcaaagggct gcagaccggg atacttcgtt ttggcattag tgagatc
//
LOCUS       DAOMXO010000753         1132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_74946_length_1132_cov_3.647168, whole genome shotgun sequence.
ACCESSION   DAOMXO010000753 DAOMXO010000000
VERSION     DAOMXO010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74946_length_1132_cov_3.647168"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLH57_12130"
     CDS             <1..293
                     /locus_tag="PLH57_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRK03401.1"
                     /translation="ELMVEKIPILGEGDIIKQYALLLFAAGITPTFFYFVGLKYTRAT
                     AGAFCEMIQAIVAVLVAWIVLGDALAWHQIIAAVVLVVSVTMINLLQARDKS"
     gene            290..1126
                     /locus_tag="PLH57_12135"
     CDS             290..1126
                     /locus_tag="PLH57_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRK03402.1"
                     /translation="MTENRSEFWKLTLVVFGLLFGWVALCRADDKTLTLKAGSWEKSY
                     TLKQLQELLPMTELNVYDPVYKKEMNFEGFLLSDVLGLMTSANLTKNLGQELVFTAED
                     GFAPSMFLPDHRNQRAIVAFGEKGKNGGWSEFETGKTTTTPAPFYVVWQSSKLKEERY
                     PRPYKLVKLEVVNLREKFPKVFPTGNTHEKVFRGFTTFKQHCLMCHSMNLEGGEVGPE
                     LNIPKNITEYRSEKTLRNFIRSAKSFRAKSKMPDFPQLTARELDGLMAYLKYMGGQKI
                     LQ"
BASE COUNT          308 a          225 c          295 g          304 t
ORIGIN      
        1 cagagttgat ggtcgaaaaa ataccgattc tgggtgaagg cgacattata aaacaatacg
       61 cgcttctttt gtttgccgcg ggcattacgc cgaccttttt ttattttgta ggacttaaat
      121 acactcgagc tacggctggc gccttctgtg aaatgattca agcgattgtt gcagttttgg
      181 tggcatggat cgtactgggt gacgcgctag cttggcacca gattatcgca gcagtggtgt
      241 tggtggtgtc ggtaaccatg attaatttat tgcaggcacg agataaatca tgactgagaa
      301 tcgttcggaa ttttggaaat taaccttagt ggtttttggg ctattgttcg gttgggtcgc
      361 gctttgtcgg gccgacgata aaacactcac gctaaaggcc ggctcttggg aaaagagcta
      421 cacattgaag cagctccaag agttgcttcc tatgacggaa ctaaatgttt atgacccggt
      481 ttataaaaag gaaatgaact ttgaggggtt cttgctgagc gatgtgttgg ggctaatgac
      541 ttcggccaac cttaccaaga acctgggcca ggaattagtg tttaccgcgg aagacgggtt
      601 tgccccgtca atgtttttgc ccgatcatcg gaaccaacga gcaattgtcg cgtttgggga
      661 aaaagggaaa aatggcggtt ggtcggaatt cgaaaccgga aaaaccacga ccactcccgc
      721 gccattttat gttgtgtggc aaagttctaa gttaaaagaa gagcgttatc ctcggcccta
      781 caaactagtt aagttggaag tggtaaactt gcgggaaaaa ttcccgaagg tgtttccgac
      841 gggtaatact catgaaaagg tttttcgggg cttcaccacg tttaagcaac attgcttaat
      901 gtgtcattca atgaatctcg agggcggcga agtgggacca gagctcaata ttccaaaaaa
      961 catcacggaa tatcgcagcg aaaaaacatt gcgaaatttt attcgcagcg cgaaatcatt
     1021 tcgcgccaag tcaaagatgc cagactttcc ccagttgact gctcgagagc ttgatggctt
     1081 gatggcgtat ctcaaataca tgggtggtca gaaaatattg cagtaaatag tt
//
LOCUS       DAOMXO010000754         1127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_75418_length_1127_cov_3.501866, whole genome shotgun sequence.
ACCESSION   DAOMXO010000754 DAOMXO010000000
VERSION     DAOMXO010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75418_length_1127_cov_3.501866"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..402)
                     /locus_tag="PLH57_12140"
     CDS             complement(196..402)
                     /locus_tag="PLH57_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03403.1"
                     /translation="MSRPLKIGEWKDKSVEAVSDAVDKVVLVSKNAATTVDKKAHKRP
                     WLFMGVAALFSSFFGFFLGRKTKK"
     gene            complement(529..>1127)
                     /locus_tag="PLH57_12145"
     CDS             complement(529..>1127)
                     /locus_tag="PLH57_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03404.1"
                     /translation="NWWRSRANLLTRMNSPEVIEWTGPHKEWLQGKITSQALASWIDS
                     FSDATNLWFESAEVAWFFLETCEELGRPVAKKVRLVWQEFFKDWASKELSATEVFHES
                     YHLSRLLPRVGVSVPMYERGLLPQHSGWNVIDCGGEGFYREAHPEKANALRERFLNDL
                     KSDGRTATTIISQSLCCVEHLAADKQGSVYWLDHLFPS"
BASE COUNT          297 a          292 c          239 g          299 t
ORIGIN      
        1 atatcgccta aaagggcggt tttaattttt tcgatgcgcg ccttgagttt accctctcga
       61 aaccagttca aaagggttgc gcagtcgtct gatgcatagg aggcatgcgc aaaaataaaa
      121 aaaccaatta ccagcaaaga agttacccgc tttgctggta tcggtttttg aaatctaaaa
      181 attcgaagat cgggactact tctttgtctt gcgaccaaga aagaacccga aaaagctcga
      241 aaacaacgcc gccacgccca tgaagagcca gggacgtttg tgggcctttt tgtctaccgt
      301 ggttgcagca tttttagaaa cgagcacgac cttatcgacc gcgtcgctca ctgcttcaac
      361 cgacttgtct ttccattcac caatttttaa cggacgtgac atacgaattc ctcctcattt
      421 agagatcgtt cgatctcgtg ggcgtttttc accctaatta ctcatcggca aaaaacaccc
      481 attacttgag tgtaattatc atttaatttt tatttagttt tcaattactt aggagggaaa
      541 aaggtgatca agccagtaca cggacccctg cttgtccgca gccaagtgtt ctacacaaca
      601 gagtgactga ctgatgattg tcgtggcggt tcggccatca cttttgaggt cgttcaaaaa
      661 acgttctcta agagcatttg ccttctctgg gtgagcctca cggtaaaacc cttcaccgcc
      721 gcaatcgatt acgttccaac cggaatgttg gggtaaaagg ccacgctcgt acattggcac
      781 tgacactccc acccgaggga gcaggcgact taagtgataa ctctcatgaa aaacttcggt
      841 tgcgctcagc tccttggaag cccagtcttt gaaaaactcc tgccatacca gccgaacttt
      901 tttggccacg gggcgtccca gctcttcaca ggtttccaag aaaaaccacg cgacctcagc
      961 tgattcaaac cacagatttg tagcgtccga aaaagaatcg atccaagacg ccagtgcctg
     1021 acttgtaatc ttaccctgca accattcttt atgcggccca gtccattcaa tgacttccgg
     1081 tgaattcatt cgggttaata agtttgcgcg gcttcgccac cagttct
//
LOCUS       DAOMXO010000755         1116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_76478_length_1116_cov_6.400566, whole genome shotgun sequence.
ACCESSION   DAOMXO010000755 DAOMXO010000000
VERSION     DAOMXO010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76478_length_1116_cov_6.400566"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLH57_12150"
     CDS             complement(<1..503)
                     /locus_tag="PLH57_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03405.1"
                     /translation="MIFFFESLFRVLTEFFGNSCAFCGETRDLARHSRICNACIDKLR
                     RPVLDMYEQHLNGLQIYSLFKYDDWVRRLILTNKRNGSDLMVHWCAERLENALRALGC
                     TPESTQIVWIPSHPRSEIHITEALALQLATRGYRLPLRIPLIEASGILKKWPKLHQTT
                     HRAQRAPL"
     gene            complement(568..>1116)
                     /locus_tag="PLH57_12155"
     CDS             complement(568..>1116)
                     /locus_tag="PLH57_12155"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRK03406.1"
                     /translation="AVDAIDKIRDTATSHDRVFLVEVMGRTSAEIAARVGLSGGAESI
                     LIPDASGVVYSEKTVRKLVRSVLRSARQGKLSSIIVVCEGEKKDATYRLAKALKLKAK
                     VEPRVAILGHVQRGGVPTAMDRTMASLMGAEAVQALLEGERDVAIGVTQGSISRILLK
                     SILGKQKKPDLKMIRLTEILAS"
BASE COUNT          259 a          272 c          290 g          295 t
ORIGIN      
        1 agcggggccc gttgggctcg atgggtcgtc tggtgaagtt tgggccactt ttttaggatc
       61 cctgaggctt cgatgagcgg aatacgaagt ggcaaacggt atccgcgcgt ggcgagttgg
      121 agtgccaggg cttcggtaat gtgtatttcg ctgcgtgggt gggatggaat ccagactatt
      181 tgagtggact ccggtgtgca accaagagcg cgaagtgcat tttccagtcg ttcggcgcac
      241 cagtgcacca ttagatccga gccgtttctt ttatttgtta aaatcagacg tctgacccag
      301 tcatcatatt taaaaagact gtaaatttgc agaccgttca agtgttgctc gtacatatca
      361 agaactggtc gtcgtaattt atcgatacag gcattacata tccgactgtg tcgagcaagg
      421 tcgcgcgttt caccgcaaaa tgcacaggag tttccgaaaa actcggtaag aactcgaaac
      481 aaagattcaa aaaaaaaaat catgctcaac cgcgaatgca aggagcgtgc gcggctagcg
      541 gcggcgaacc tctaaggtcc taaattatta actggctaga atctcggtta agcgaatcat
      601 ctttaggtca ggtttctttt gcttgccaag aattgatttt agcaagatgc gggagatcga
      661 cccctgggtt acgcctatag cgacgtcgcg ttctccttcg agtagcgcct gaacagcttc
      721 ggcccccatg agtgatgcca ttgttcgatc catagcggtt ggtaccccgc cccgttgtac
      781 gtgaccaagt atggcaactc tcggttcgac tttggctttt agtttaagtg ccttggccag
      841 tcgataagtg gcgtcttttt tttcaccttc gcaaacaaca atgatgctcg agagtttccc
      901 ttggcgtgcg cttcttagaa cgcttcgtac cagtttacgc acagtttttt cgctataaac
      961 aacccctgaa gcatcgggaa taagaatgct ttcggcgcca ccgctcaatc cgactcgtgc
     1021 ggcgatttcc gcactggtgc ggcccatgac ctcgactaaa aaaactcgat cgtgcgaggt
     1081 tgcggtgtcg cgaatcttgt cgatcgcgtc cactgc
//
LOCUS       DAOMXO010000756         1116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_76489_length_1116_cov_5.215834, whole genome shotgun sequence.
ACCESSION   DAOMXO010000756 DAOMXO010000000
VERSION     DAOMXO010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76489_length_1116_cov_5.215834"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLH57_12160"
     CDS             complement(<1..242)
                     /locus_tag="PLH57_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03407.1"
                     /translation="MNRIFIFAVSLFFASASAWANSVEAEPTTCRKLLRANEIRQDAA
                     LFEMFPLPINGPHQVEFLNLRHRFLARVRAIRIENI"
     gene            complement(312..>1116)
                     /locus_tag="PLH57_12165"
     CDS             complement(312..>1116)
                     /locus_tag="PLH57_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03408.1"
                     /translation="LAGLTSATAEVDALLSFSRLLSASTAEKPYTLPHIIEDSEQAPV
                     ATQVSIIDGRNALLDLSKPEVPVPNHAVLAADQSLGDRVLFLTGENAGGKTTYVRQIA
                     QAVVLAQVGAPVPATSARLTPVLLLSTMQRADSTLDGTSLFRNEIRRVKALIDALDGT
                     RHALVLTDELFSGTSHDQKIAAETAFLRYLHGRGLLAIASSHSHELGNLEAELGGFRN
                     IQIGGLSEHPFKVTRGVSPVRNALKIMAEEQLPDELRRLAEDEYGFKKD"
BASE COUNT          269 a          291 c          298 g          258 t
ORIGIN      
        1 tctatgtttt caatgcgaat ggctcggact cgggccaaaa atcgatgccg aaggtttaaa
       61 aattctactt gatggggccc gttaattggt aacggaaaca tttcaaacaa cgcagcatct
      121 tggcgaattt cattggcacg aagcagtttg cgacaagtcg ttggttccgc ctccaccgag
      181 ttggcccatg cggacgcgct tgcgaaaaac agcgatactg caaaaatgaa aatccgattc
      241 ataatgcagg gttagtaaca cgacgcggca tattctgtca ataaaggtca agtataggga
      301 ttggccgcta actagtcttt tttaaagcca tattcgtctt cagctagacg tcttagttcg
      361 tcgggcagct gttcttcggc catgatctta agcgcatttc ggaccggtga aaccccgcgg
      421 gtgaccttaa aggggtgctc acttaaaccg ccgatttgaa tatttctaaa accaccaagt
      481 tcggcctcaa ggtttccaag ctcgtgactg tggctgctgg caatggcgag caggccgcgc
      541 ccgtgcaaat accgcaaaaa tgccgtctca gccgcgattt tctgatcgtg ggaagtgcca
      601 gagaatagtt cgtcggttag caccaacgcg tggcgtgtgc catcgagtgc gtcgataagg
      661 gctttaactc ggcgaatttc gtttctaaac aaactggtgc cgtcgagcgt ggagtcggcc
      721 cgttgcatgg tgctcaataa cagcaccgga gtcaggcgcg cactcgtagc gggcacgggc
      781 gcccccacct gagcaagtac gacagcctgt gcgatttgac gaacgtaagt ggttttacca
      841 cccgcgttct caccagttaa aaataaaacc cggtcgccca aactctgatc agcggcaagc
      901 acagcgtggt tcggcaccgg cacttccggt ttgctcaaat ccagcagtgc attgcgccca
      961 tcaataatgc tcacttgagt ggctaccggc gcctgctccg agtcttcgat aatgtgcgga
     1021 agcgtgtagg gtttctcggc cgtcgaagca ctgagtaacc gcgaaaacga aagtagtgcg
     1081 tcgacctcgg cagtggctga cgttaagccc gctagc
//
LOCUS       DAOMXO010000757         1115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_76621_length_1115_cov_4.103774, whole genome shotgun sequence.
ACCESSION   DAOMXO010000757 DAOMXO010000000
VERSION     DAOMXO010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76621_length_1115_cov_4.103774"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1115
                     /locus_tag="PLH57_12170"
     CDS             <1..>1115
                     /locus_tag="PLH57_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03409.1"
                     /translation="HWIDRPVKEYSFTGPGTQLPEVVVTNAMRELEFQYDPSTKELKI
                     LKVQWDSKNQRRQITHEHFFKGVELTTESNHFLSDSDFALDIGKTIDNAEGRREYSIK
                     APVTVLFSSRPKIMPTGETIPSGIRAFLWPILTELVFKAPVPSMVVVPQPPARVVDME
                     FLTPEKTPQELQGAQSGDLRVSLEVEPPYGKGDSMVVNTLVSRKEIHSLMVEHLRQMF
                     YGLVALSPSPEIVDQIASDLLTIENQKAKDVSEVNAERETILDLGRSSLPGELGKQIA
                     LSTNLSGLYDLVRTGGGLDALNATPRHQLPYDQWQRENEVIRLTRDAEKEIGAPAKPW
                     RQILVESYEAGGGPVAAQVAKVEKKQTTAVKKYVGGM"
BASE COUNT          311 a          260 c          273 g          271 t
ORIGIN      
        1 ccattggatc gatcgcccag tgaaggaata ctcctttact ggtcctggaa ctcagctgcc
       61 cgaggtggtc gtgacgaacg caatgaggga gctcgagttt cagtacgacc catctactaa
      121 agaattaaaa attttaaagg ttcagtggga ctccaagaat caacgtcgcc aaattacgca
      181 cgaacacttt ttcaaaggtg tagagcttac aactgagtct aaccatttcc tctcggactc
      241 tgatttcgcg ctagatatag gcaaaactat cgataacgct gaaggtcgtc gcgaatacag
      301 cattaaagca ccagtaaccg tacttttttc ttctcgcccg aaaattatgc caaccggcga
      361 gaccatccct tctggaattc gtgcctttct gtggcctatt ttgacagagc tagtttttaa
      421 agccccggtt ccttcgatgg tcgtggttcc tcagccgcca gcgcgagttg ttgacatgga
      481 gtttctcact cctgaaaaaa ctccccaaga acttcaaggc gcacaaagtg gcgatttaag
      541 agtgtcgctc gaagtagaac ccccctacgg gaaaggggat tcgatggtag tgaacacgtt
      601 agttagtcgc aaagaaattc attcgttgat ggtcgaacat ctacgccaga tgttttatgg
      661 gctcgttgcg ctttctccaa gtccggaaat cgtagaccaa attgcgtctg atctcctcac
      721 tattgaaaat cagaaagcaa aagacgttag cgaagtaaac gccgaacgcg aaaccatatt
      781 agacttaggt agatcctctc ttcctggaga actcggcaaa caaatagcgc tttcgaccaa
      841 tctttctgga ttgtatgatc tcgttcgaac tggcgggggg ttagatgcgt tgaatgcgac
      901 tcctagacac cagctaccat atgaccaatg gcagcgagaa aacgaagtta tccgtttaac
      961 tcgtgatgca gagaaggaga ttggtgcacc tgcgaaacct tggcggcaga tcttggttga
     1021 aagttacgaa gcgggtggtg gcccggtagc ggctcaagtt gcaaaggtcg aaaaaaagca
     1081 gacgaccgca gtaaaaaaat acgtgggagg catgg
//
LOCUS       DAOMXO010000758         1114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_76712_length_1114_cov_4.353163, whole genome shotgun sequence.
ACCESSION   DAOMXO010000758 DAOMXO010000000
VERSION     DAOMXO010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76712_length_1114_cov_4.353163"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..>1114)
                     /locus_tag="PLH57_12175"
     CDS             complement(261..>1114)
                     /locus_tag="PLH57_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRK03410.1"
                     /translation="ENLKGELLLDQLLVRLKDFSMKIFDGTVAGQASFNARNAKPETA
                     TNLVVTGLESKKMVESQMPFAKNTIQGTVSAKLNIGGSGVNPTDMTSSWSGSGTMNVK
                     EAVFATLDLGRQIKDGALGKLPEALRNKIGVSDKVLDWKGEYQTVEAKYNLANGVFNL
                     VELTGKAYPNKGMDLKGSGKVQLKNYGLDLGVDFIDTYNWLNADQYVKDERYGHFALP
                     AKVGGTLFAPKFDWGAALGKLAQGAAKQQAKDALKKGILDKVKLPGSGDAKPSDAVKG
                     LMKGLFN"
BASE COUNT          263 a          307 c          221 g          323 t
ORIGIN      
        1 ggtgccactg attttcgcgc gatttacatt tttgtcggta ccgcaacccg acgaaagcac
       61 tgctacagcg accgcacccg caacaacgac ggcggcccaa aaactgtaat tttgcttgcg
      121 aggtttcgaa tcttctactc gacgaataat ttgtgttccg aaggtcatcg cggttctccc
      181 tactttcact tttaaaactt acccctctta caccatagct ccgagtcaat cgtcaaataa
      241 cactcagagt cgtttggtta ttaattaaag agacctttca taagcccctt caccgcgtcg
      301 gaaggctttg catccccgct tccgggcaac tttaccttgt ctaaaatacc ctttttcagc
      361 gcgtctttag cctgctgctt cgctgcgccc tgcgcaagtt tgcctaacgc cgcaccccag
      421 tcaaatttag gagcaaacaa cgtcccccca actttagcag gtagcgcaaa atgaccataa
      481 cgctcgtctt ttacatattg atcggcgttc aaccagttat aggtgtcgat aaaatccact
      541 ccgagatcca gtccgtagtt tttcaactga acttttccgc tacctttaag gtccatgcct
      601 ttattgggat aggccttgcc agtgagttcc actaaattaa acaccccgtt ggcgagatta
      661 tacttggctt ctacggtttg atattcgccc ttccagtcca gaactttgtc ggaaacacca
      721 attttatttc gcaaggcttc gggtagtttt ccgagcgcac cgtctttgat ctgccgccca
      781 agatcaagcg tagcgaaaac cgcttctttg acgttcattg ttccactgcc actccatgac
      841 gaggtcatgt cagttggatt cacgccgcta ccgccaatat ttaatttggc gcttacggtg
      901 ccctgaatcg tattcttggc aaatggcatt tggctttcga ccattttttt actctctaaa
      961 ccggtgacca caagattggt ggcggtctcg ggtttagcgt ttctggcatt aaagctagct
     1021 tgtcccgcaa cagtcccatc aaaaattttc atggagaaat ctttcagacg aaccaaaagt
     1081 tgatcgagca acagttcgcc tttcagattt tcca
//
LOCUS       DAOMXO010000759         1109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_77205_length_1109_cov_2.688805, whole genome shotgun sequence.
ACCESSION   DAOMXO010000759 DAOMXO010000000
VERSION     DAOMXO010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77205_length_1109_cov_2.688805"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLH57_12180"
     CDS             complement(<1..242)
                     /locus_tag="PLH57_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03411.1"
                     /translation="MTARSHTPKRVVLPVLFLVMFFHACSASYKDIGSPTGRKAIMDD
                     AINYLTDGNCESAITTLQPLYISRWVDNEVRLLYASA"
     gene            complement(372..>1109)
                     /locus_tag="PLH57_12185"
     CDS             complement(372..>1109)
                     /locus_tag="PLH57_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRK03412.1"
                     /translation="ARFKVTPEALKAAITKRTKAFIINSPSNPTGMMYSKSELEALAR
                     VLEGTNVWVLSDEIYEKLVYEGQFVSFASLSKDAFSRTITSNGFSKSYAITGWRAGYA
                     AGPKNVIDAMAMIQGQSTSGIASIVQKGALAALAVPNSEIEKEMLSLRKRRNLMCEVL
                     RKAEDLKFLTPDGAFYVFVDVSAYLARKPGLTTDEFALALLKNECVGTVPGSGFGADG
                     YLRLSFAVSEAEVREGTERLVAALKKI"
BASE COUNT          291 a          283 c          256 g          279 t
ORIGIN      
        1 gctgaagcat agagtaagcg gacctcgtta tctacccacc gagaaatgta caacggttgc
       61 agagttgtaa tggctgactc gcagtttccg tccgtaaggt agttaatcgc atcgtccatg
      121 atcgcttttc gaccggtggg cgaaccaatg tctttgtaac ttgcactgca tgcatgaaaa
      181 aacattacta aaaataatac cggtaaaacc acgcgcttag gcgtgtgcga acgcgctgtc
      241 ataggacgca gccctcctaa tagtaatgat agtttttttt agcgagagac tgaaccgtgc
      301 aactcctgcc ggcacaaggg ttgcatcttt tgctaggtag aaaattccgg tcgaatcaac
      361 gttcccgtta cttagatttt tttaagcgcc gccaccagac gctctgtgcc ttcacgcact
      421 tctgcctcac tcacggcgaa gctcaagcgc aagtatccat cggccccaaa cccactgcct
      481 ggaactgttc caacacactc attctttaaa agcgcgagag caaattcatc ggtagtgagc
      541 cccggttttc gcgcaagata cgccgaaaca tcaacaaaaa catagaaagc accatcggga
      601 gtcagaaact tgaggtcctc ggctttacga agcacttcac acattaggtt tcggcgtttt
      661 cgcaacgaaa gcatttcttt ttcgatttcg gaatttggaa cggcaagggc cgcaagagca
      721 cctttttgaa caatcgaagc tatgccagag gtgctctgcc cttgaatcat agccatcgca
      781 tcgattacgt ttttgggacc agcggcgtaa ccggcacgcc agcctgtgat tgcgtagctt
      841 ttcgaaaaac cgttggacgt gatcgtacgc gaaaaagcat ctttcgaaag acttgcgaac
      901 gaaacgaatt gcccctcata caccaatttt tcgtaaatct cgtcggagag cacccacaca
      961 tttgtgccct cgagcactct ggcgagtgct tccagctcgg atttcgaata catcattccg
     1021 gtgggatttg aaggactgtt gataataaag gctttagttc gtttagtgat cgcggccttt
     1081 aacgcttcgg gagtaacttt aaagcgagc
//
LOCUS       DAOMXO010000760         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_77973_length_1101_cov_4.464627, whole genome shotgun sequence.
ACCESSION   DAOMXO010000760 DAOMXO010000000
VERSION     DAOMXO010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77973_length_1101_cov_4.464627"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLH57_12190"
     CDS             <1..911
                     /locus_tag="PLH57_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK03413.1"
                     /translation="RFALTTSTVANGSMAKAKNILTDDFKPTREVSERGMKLLSSPGV
                     EFSYIAFNQTDPVVGGPKRKFLRQAMSLAYDEAPTIGMFYLGLGKKAESMIPPGVFGY
                     DSEYKNKYREFNVAKAQELLAKNGFPEGKGLPELTYDLRSDPTARQVAEYFVRSMAKI
                     GIRIKLNQVSWPELNSRIAKKQAQVWGGIVWVYDYPDPENGLQLLYGPREAPGSNKTS
                     YKNPAYDVLFEKFIRNRDDKERLALIKRMRSIYEEDVPWIVAVHRNENRLTHSWLHNL
                     KVHAFEYNSDKYLRVDAEARKRVLER"
     gene            908..>1101
                     /locus_tag="PLH57_12195"
     CDS             908..>1101
                     /locus_tag="PLH57_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012472415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK03414.1"
                     /translation="MMAFVIRRLLYAIPTLFGVCLIIFTLFNLVGGDPAYQMLGKHAT
                     ETQILELRRELGLDKSKVEQ"
BASE COUNT          305 a          241 c          276 g          279 t
ORIGIN      
        1 ttcgtttcgc tctgacgacg agtacggtgg cgaatgggtc gatggcgaag gccaaaaata
       61 ttctgaccga cgactttaag cccacccgtg aagtaagtga acggggcatg aagttgttga
      121 gttccccagg ggttgaattt agttacatcg cattcaatca aacagaccct gttgttggtg
      181 ggccaaaacg caaattttta cgacaagcta tgtcgcttgc gtacgacgaa gcccccacga
      241 ttggaatgtt ttatctcggc ttgggaaaaa aagccgagag catgattcct ccgggggtgt
      301 tcggctacga ttccgaatat aaaaataaat atcgcgaatt taatgtcgcg aaagcgcaag
      361 agctactagc aaaaaacgga ttcccagaag gtaaaggact gcccgaactc acttatgacc
      421 ttcggtcgga tcctacggct cgccaagtgg cggaatattt tgttcgatcc atggctaaaa
      481 tcgggattcg gatcaaactc aatcaggttt cttggcctga attgaattca cgaattgcca
      541 aaaaacaagc tcaagtctgg ggcggaatag tttgggttta cgattacccc gacccagaaa
      601 acggacttca actattgtac ggtcctcgcg aagctccggg ctctaataaa acgagctaca
      661 aaaatccagc ctatgatgtg ttgttcgaaa aattcattcg aaatcgtgat gataaagaac
      721 gcctggcgtt gatcaagcgg atgcgaagca tttacgagga agatgtgcct tggattgtag
      781 ccgtgcaccg aaatgagaat cgtctgactc attcttggct tcacaatttg aaagtccatg
      841 cattcgaata caactccgac aagtacctgc gcgtcgatgc cgaagcgaga aagagagtgc
      901 ttgaacgatg atggctttcg tcattcgtcg tttactgtac gctattccta cattgttcgg
      961 ggtttgcctt attatcttta ccctgtttaa tttggtcggt ggggaccccg cttatcagat
     1021 gcttggtaaa cacgcgaccg aaactcaaat cctagagctg aggagggaat tggggctcga
     1081 taaaagcaag gttgagcagt t
//
LOCUS       DAOMXO010000761         1100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_78058_length_1100_cov_5.050718, whole genome shotgun sequence.
ACCESSION   DAOMXO010000761 DAOMXO010000000
VERSION     DAOMXO010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78058_length_1100_cov_5.050718"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLH57_12200"
     CDS             complement(<1..290)
                     /locus_tag="PLH57_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRK03415.1"
                     /translation="MSTYNPRFYIEDAAKHDGQTVTIHGWVYNKRSSGKIKFLQLRDG
                     TGLMQGVLVKGECEDTSFEEADKLTQESSVIVTGKLRKEPRSPGGYEMGVLN"
     gene            complement(294..>1100)
                     /locus_tag="PLH57_12205"
     CDS             complement(294..>1100)
                     /locus_tag="PLH57_12205"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HRK03416.1"
                     /translation="LLGVGQGSAHPPRLIVLEYSPKAAKKAKTVALVGKGITFDSGGI
                     SIKPSSRMEDMKHDMCGAAAVIGAICLAALNKVEHRVIAVVAAAENMPSGQAICPGNI
                     LVSRAGKTVEITNTDAEGRLVLADALDFVQDLNPDYIVDAATLTGAVSITLGKACSGL
                     MANDAGIVKKLRDAALDSGERVWELPMYEEYFDDLKSDYADMRNSGDTASHGTAKGAK
                     FLEQFIRKGVRWAHLDIAAVAYNLGSIPYMAKKGATGYGVRLLNGFLSEI"
BASE COUNT          238 a          296 c          276 g          290 t
ORIGIN      
        1 atatttaaaa cgcccatttc gtagccgccc ggtgaacgtg gttctttacg cagttttccg
       61 gtcacgataa cagaactctc ttgggtgagt ttatcggctt cttcaaagct ggtgtcttcg
      121 cactcgcctt tgacaagaac tccttgcatg agtccggttc catcgcgtaa ttggagaaac
      181 ttaatttttc cactactgcg cttgttatag acccagccgt gtatggtaac agtttggccg
      241 tcgtgtttgg cagcgtcttc aatataaaaa cgtggattat aggtgctcat aatttaaatt
      301 tccgacaaga atccatttaa caggcgtacc ccgtacccag tcgcgccttt tttggccatg
      361 tacggaatgg aaccgaggtt gtacgccacg gcggcaatgt cgaggtgggc ccagcgaacc
      421 cccttgcgaa tgaattgttc caaaaacttt gcgcctttcg cggtaccgtg ggatgcggtg
      481 tcgccagagt ttcgcatgtc tgcgtaatca ctcttgaggt cgtcaaaata ttcttcgtac
      541 attggtagtt cccatacgcg ttcacccgag tcgagtgcag cgtcacgaag tttttttaca
      601 atgcccgcgt cgttggccat tagtcctgag caggctttcc cgagggttat agacaccgca
      661 ccggttaggg tggcggcatc gacaatgtag tcggggttca gatcttgaac gaaatcaagg
      721 gcgtccgcca gcactagtcg accctcagcg tcggtgttgg ttatttccac agtttttcca
      781 gcgcgcgaaa ccaatatatt gcctgggcaa atcgcctggc ccgagggcat attctcagcc
      841 gcagcaacca ccgcgatcac tcgatgttcc accttattga gcgccgccaa acagatggcc
      901 cctattacgg cggcggcccc acacatgtcg tgtttcatgt cttccatgcg ggacgaaggt
      961 ttgatgctaa ttccgcccga atcaaacgta atacccttgc ctacgagcgc tactgttttc
     1021 gcttttttcg cggccttagg ggaatactcc aagacgatca aacgtggagg gtgggcgctg
     1081 ccttggccga cgcccaacaa
//
LOCUS       DAOMXO010000762         1098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_78275_length_1098_cov_3.602109, whole genome shotgun sequence.
ACCESSION   DAOMXO010000762 DAOMXO010000000
VERSION     DAOMXO010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78275_length_1098_cov_3.602109"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLH57_12210"
     CDS             <1..686
                     /locus_tag="PLH57_12210"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRK03417.1"
                     /translation="QLKPVVSAKWKPLDIPEGTLVLVDKKGFSESHVRLIARGPKRKV
                     PEYHDLLVAENILGGTFGSRLTTLLREKLNLTYSVDVNFEFGRELGVFEIATSTRNER
                     LLELLNVLEGELRGFVNDGVKKTEVESAKEYLLGSFPIGLQNSFLVAQRYFRLLLEGL
                     SPKFLDEYPEHIRAVSQESVNAAIRKYFSYDRFISVVVCDAERTKESLKGRKSPVKVV
                     SAKQVIETY"
     gene            complement(703..>1098)
                     /locus_tag="PLH57_12215"
     CDS             complement(703..>1098)
                     /locus_tag="PLH57_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03418.1"
                     /translation="FPQVDVSAARGKAGRGERRRAFLRVIELSEYDAWMNESLKLSGA
                     QHGLKKNRGLKKVLERSSRFMTSAMHLFGVTPSGPQRRGRIVYDLRLGQAPIHSGTIE
                     SLRRNVSKVSEKIEGIHLLTNSELVLIEP"
BASE COUNT          291 a          230 c          268 g          309 t
ORIGIN      
        1 aacaactgaa gccagtcgta agtgcgaaat ggaaaccact tgatattcca gaaggaactt
       61 tagtgctggt ggataaaaaa ggattttcag aaagtcacgt gcgcctgatc gcccgaggcc
      121 cgaagcgaaa ggtccccgag tatcacgatc tgttggtagc cgaaaacatt ttgggcggta
      181 catttgggtc gcgattgacc acgttgttac gcgaaaaact aaatttaacg tattccgtag
      241 atgtaaattt cgaatttggt cgtgaactag gagtttttga aatagcaact tcgacccgca
      301 acgaacgcct tttagaactt ctcaatgtat tagaagggga attgcgtggc tttgttaatg
      361 acggagttaa aaagaccgaa gtcgagtccg ctaaagagta tttattagga tcttttccaa
      421 ttgggctgca aaattctttt ttagttgctc aaaggtactt tcgattactt ctagaggggc
      481 tttcgccaaa atttctagac gagtatcccg agcatattcg agctgtttca caggaaagcg
      541 ttaacgctgc aattcgaaaa tacttttcgt atgatcgatt tatttcggta gtggtatgtg
      601 atgcggaacg cactaaagaa tcactgaagg gtcgaaagag tcctgtcaaa gtagtttccg
      661 ccaaacaagt aattgaaacc tactaacgga gaactcgaat tattagggtt cgatcagcac
      721 caattcgcta ttggtaagaa ggtgaattcc ttcgatcttt tctgaaactt tagaaacgtt
      781 ccttcttagc gattcaattg tgccggagtg gattggcgct tggcccagtc gtaggtcgta
      841 tactattctt cccctgcgtt ggggcccgct aggtgttacc ccgaacaagt gcattgcgga
      901 ggtcataaat ctcgatgaac gctctaaaac tttttttagt cctcggtttt ttttgaggcc
      961 gtgttgagcg cctgatagtt ttagggactc attcatccag gcgtcatatt ctgagagctc
     1021 gattacgcgc agaaacgccc gacggcgttc accgcggccg gctttcccgc gcgcggcaga
     1081 tacatcgacc tgtgggaa
//
LOCUS       DAOMXO010000763         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_78465_length_1096_cov_3.834774, whole genome shotgun sequence.
ACCESSION   DAOMXO010000763 DAOMXO010000000
VERSION     DAOMXO010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78465_length_1096_cov_3.834774"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..623
                     /gene="gmk"
                     /locus_tag="PLH57_12220"
     CDS             33..623
                     /gene="gmk"
                     /locus_tag="PLH57_12220"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRK03419.1"
                     /translation="MSNKRPTASNAKLIVLSAPSGAGKSTLCTLLLAKHPRFKISISH
                     TTRKPRGEEQHGTHYFFISEKEFQEMISRNDFLEYAHVFGRSWYGTSKQQVEDALAQN
                     FHVVFDIDVQGARALKQIYGKRCVTIFVLPPSFEELEKRLRARATESESAIQSRLETA
                     KKELREATHFDHQIVNQELSQAMKELETILTEEDCL"
     gene            614..>1096
                     /locus_tag="PLH57_12225"
     CDS             614..>1096
                     /locus_tag="PLH57_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRK03420.1"
                     /translation="MSLSKHADVTIDDVCNAVLAYYPDADVSVLKRAHEFAEKVHGDQ
                     KRHSGEPYMVHPRSVAFILTELRLDVASIATAILHDTVEDTHATLEEVEKQFGTTIAT
                     LVDGVTKLSKITFKTSEEKQAENFRKMIIAMAKDIRVILVKLADRLHNMRTLEHLLPY
                     K"
BASE COUNT          329 a          274 c          233 g          260 t
ORIGIN      
        1 acttcttgag aatctcaagg aacaactagc gaatgtcgaa taaacggcca acggcatcga
       61 atgcaaaact tattgtgctt tcggccccga gcggtgccgg taaaagcact ctctgcactt
      121 tgcttctggc gaaacaccca agatttaaaa tttcaatttc tcacaccact cgtaagcctc
      181 gcggggaaga acaacacggc acgcactact tttttataag tgaaaaagaa ttccaagaaa
      241 tgattagtcg caacgatttt ttggaatacg ctcatgtttt cggccgatct tggtacggca
      301 cttcgaaaca acaagtcgaa gatgcgctcg cccaaaattt tcatgtggtt ttcgacatcg
      361 atgtgcaagg cgcccgggca ctaaaacaaa tctatggtaa acgctgcgta actatatttg
      421 ttttgccgcc ctcgtttgaa gaactcgaaa agcgtttaag agctcgcgcc actgagtcgg
      481 aatcagcaat tcagagtcgc ttggaaactg ccaagaagga acttcgagag gccacccatt
      541 tcgatcacca aatcgtgaat caagaacttt ctcaagctat gaaagaactc gaaactatcc
      601 tgactgagga ggattgtctc tgagtaagca tgctgacgtc actatcgacg acgtttgtaa
      661 cgccgtcttg gcttattacc ccgatgccga tgttagcgtc ttaaaacgag ctcatgaatt
      721 tgctgaaaaa gtacacggtg atcaaaagcg gcattccggt gaaccctaca tggtgcatcc
      781 gcgatccgtc gcgttcattc tgaccgaatt gcgactcgac gttgctagta ttgcgaccgc
      841 tattctccac gacaccgtcg aagacactca cgccacacta gaagaggtgg aaaaacaatt
      901 cggcaccaca atagccaccc ttgtagacgg ggtcaccaaa ctctcaaaaa tcacctttaa
      961 aacgagtgaa gaaaaacagg ctgaaaactt tcgcaaaatg atcattgcga tggccaaaga
     1021 cattcgagtt attttggtca aattagcaga tcgtctacac aacatgcgaa cactcgaaca
     1081 tctgctcccc tacaag
//
LOCUS       DAOMXO010000764         1093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_78737_length_1093_cov_5.342004, whole genome shotgun sequence.
ACCESSION   DAOMXO010000764 DAOMXO010000000
VERSION     DAOMXO010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78737_length_1093_cov_5.342004"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLH57_12230"
     CDS             complement(<1..617)
                     /locus_tag="PLH57_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03421.1"
                     /translation="MSANNDDSIIIRGAKEHNLKNISLRIPRDSLTVITGLSGSGKSS
                     LAFDTIFAEGQRRYIESLSTYARQFLQAQNKPDVESIDGLSPTISIQQKTTSHNPRST
                     VGTVTEVYDYLRLLFARISIPFCYQCGKSISGQTAQQIVDQILEFQQGSKMSILAPVV
                     RGRKGEYQKELASYRQKGFSRIRIDGEILDLSQNIELDKQKKHDI"
     gene            complement(601..>1093)
                     /locus_tag="PLH57_12235"
     CDS             complement(601..>1093)
                     /locus_tag="PLH57_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HRK03422.1"
                     /translation="NPIMWKTGHSLIKSKMKEEKAELAGEMSGHMFFSDRYYGYDDAV
                     YAAARLVEIVSRTTSSVSSLLSDLPTAVSTPEIRVDCPDEIKFDVVNSVKNYLAKQYS
                     VVDIDGVRVEMPEGWGLLRASNTQPVVVMRFEANSDSALKKIRSIVEAAFDGALKDYV
                     GQQ"
BASE COUNT          290 a          234 c          240 g          329 t
ORIGIN      
        1 tcgatgtcat gttttttttg tttatccaat tcaatatttt gcgaaaggtc tagtatttct
       61 ccgtcaatac gaattcggga aaaacctttt tgtcgatagg aggcaagttc tttttgatac
      121 tcgccttttc ttccccgaac cacgggtgcg agtatgctca ttttggaacc ttgttgaaat
      181 tccaatattt gatctacaat ttgctgggca gtttgccccg aaatagattt tccgcattga
      241 tagcaaaaag gtatcgagat tcgagcgaac aagagtcgaa ggtaatcgta tacttcagtg
      301 acagttccta cggttgagcg tggattatgg gaggtggttt tttgttggat tgatatcgta
      361 ggagacaacc cgtcgatgct ttccacgtct ggcttatttt gagcctgcaa gaattgacgg
      421 gcgtaggtag aaagcgattc tatgtatcgg cgctgacctt ctgcaaaaat ggtgtcaaaa
      481 gcgagagaag attttccaga cccactaagg cctgtgatca ctgtcagcga atcgcggggg
      541 atccgtagcg aaatattttt aagattatgc tcttttgccc cacgtataat aatggagtca
      601 tcattgttgg ccgacataat cttttaaagc tccatcgaaa gccgcttcaa cgatagagcg
      661 aattttttta agggcagaat cggaattagc ttcgaatctc atcacaacaa cgggttgtgt
      721 attcgaggcg cgcagtaaac cccagccctc tggcatttca actcgcacac catcaatatc
      781 caccactgag tactgcttag ctaaataatt cttaacgcta tttactacgt cgaactttat
      841 ttcatcggga caatctacgc gtatttcagg cgtgcttact gccgttggaa gatcggacaa
      901 caaagaagaa acggaacttg ttgttcgtga aacaatttct actagccgtg cggctgcgta
      961 aactgcgtcg tcgtagccat aataacgatc cgaaaaaaac atgtgcccac tcatctcacc
     1021 agctaattcc gctttttctt ctttcatctt agatttgatg agagaatgac cggttttcca
     1081 catgataggg ttg
//
LOCUS       DAOMXO010000765         1093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_78799_length_1093_cov_2.697495, whole genome shotgun sequence.
ACCESSION   DAOMXO010000765 DAOMXO010000000
VERSION     DAOMXO010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78799_length_1093_cov_2.697495"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLH57_12240"
     CDS             complement(<1..1001)
                     /locus_tag="PLH57_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03423.1"
                     /translation="MNKKRNETIDSAMLLLYEAISEKGLSPAPDILVGFSVGFRTEGE
                     YQLNLIETVNSPEKAKHLLKKLRQKNTEIFSIEIGTPQEEMIVAIPERGSILAGLRYD
                     SGPNVSILRAIDRLDGTEIREQVDFIQQRLLAFQKLRGEILDAIKNNYTAAKLNNILD
                     YQTGDRSLKAIADSYKGLYGWGESRKRGFWNDRIRTSAHLPNIGTGLENADALDRRPA
                     PLKKGFFSRVLDRVSQIESRSRRNRSTTIDSTIAPGLITKGAPASNTPKQNAADHPDL
                     FEVQNLDSHSAPRYFYVSNDIENRKEFNLPNKAPRSSLRVTSSTALPHSTNGFLIIPR
                     "
BASE COUNT          268 a          231 c          254 g          340 t
ORIGIN      
        1 cgcggaatta ttaaaaagcc attcgttgaa tgtggcaatg ctgtactcga agtcacgcga
       61 agtgacgaac gaggagcttt gttgggtaaa ttaaactctt ttcgattttc gatatcgttc
      121 gacacgtaga aatagcgagg ggcgctatgc gaatctagat tctggacctc gaacaaatcc
      181 ggatgatcgg cagcgttttg cttgggtgtg ttcgaggccg gggctccttt tgtaattaaa
      241 ccaggcgcga ttgttgaatc gatggtcgtc gatctattcc gtcgcgatct cgattctatt
      301 tggctcacac gatccaatac acgagaaaaa aaacctttct ttagaggtgc ggggcgacga
      361 tctaacgcgt cggcattttc caagccggta ccgatattcg gcaagtgggc actggtccga
      421 attcgatcgt tccaaaatcc gcgtttgcga ctttcgcccc agccatagag ccctttatag
      481 ctatccgcaa ttgcttttag agagcggtcc cccgtttggt agtcgaggat gttatttaac
      541 ttggctgccg tgtaattatt tttaatggca tctagaattt cacctcgtag cttttgaaaa
      601 gcgagaagcc gttgttgaat aaaatctact tgttctcgaa tttcagtacc gtcgagtctg
      661 tcgatggctc ttaagatact tacattgggg ccagaatcat atcttagacc agccaaaatt
      721 gaaccgcgtt caggaattgc aacgatcatt tcttcttgtg gtgttccaat ttcaatggag
      781 aaaatttcag tatttttttg acgcaatttt tttaaaaggt gttttgcttt ttcgggacta
      841 ttcacagttt ctataagatt tagctggtat tcgccttcgg tgcgaaaccc aacgctaaac
      901 ccaactaaaa tgtcgggtgc tgggcttagg cctttttcgg aaatggcctc ataaagcaaa
      961 agcatcgcgc tatcgatggt ttcatttcgt ttcttgttca aatcttcgag taaagcctga
     1021 aacaagactt tgtcggagct ccccgttcga gggtccgtta atgaattttc gaatcggttg
     1081 tatattttct gat
//
LOCUS       DAOMXO010000766         1082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_79875_length_1082_cov_6.144109, whole genome shotgun sequence.
ACCESSION   DAOMXO010000766 DAOMXO010000000
VERSION     DAOMXO010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79875_length_1082_cov_6.144109"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLH57_12245"
     CDS             <1..647
                     /locus_tag="PLH57_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRK03424.1"
                     /translation="ARTRKQLPVFKYSFGHQGPRALIIGGVHGDEPEGVIAAQGVLAA
                     LTTSFNYKLQVTVIPEFNPEGILNKMRMNSAGVDLNRNLPTKDWSPVAAKERYFPGKS
                     ANSEPENQALVQLLDAQKFDLIISAHSWNPMININGPACEPEAEVIKKHTGYTIEPDI
                     GYPTPGSLGTYGGKERGISTITFEIQKDIDFHEINKVHVPALLDCLRQTEKRNQ"
     gene            700..>1082
                     /locus_tag="PLH57_12250"
     CDS             700..>1082
                     /locus_tag="PLH57_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRK03425.1"
                     /translation="MNVNDLSSEDTSAIREVIQLLDHGKLRVAEKIPGATGPQSWKVN
                     EWVKKAILQYFRIQKMQLMETNEFNYFDKIAPKKWTGSEGVRVVPPAIARYGSFLNSG
                     CIMMPSYVNIGAYVDSGTMVDTWATV"
BASE COUNT          339 a          260 c          236 g          247 t
ORIGIN      
        1 ttgcccgcac tcgaaaacaa ctccccgtat tcaaatactc tttcggccac caaggccctc
       61 gtgctttgat tattggtggc gtacatggcg atgaacccga aggcgttatt gccgctcaag
      121 gcgtattggc tgcactaaca acttcgttta actacaaact gcaagtgact gtgattcctg
      181 aattcaaccc ggaaggcatt ctaaataaaa tgcgcatgaa ctctgccggt gtggacttaa
      241 acagaaatct gcccaccaaa gactggtcgc ccgtagctgc taaagaacgc tactttccag
      301 gaaaatcggc taatagcgaa cccgaaaacc aagccttagt gcaattgctc gatgctcaaa
      361 agtttgattt aatcattagc gcgcactctt ggaatccaat gatcaatatc aacggcccag
      421 cttgcgagcc cgaagccgag gtaatcaaaa agcacaccgg atatacgatc gaacccgata
      481 ttggttatcc gacaccagga agccttggca cctatggcgg caaagagcgc ggaatttcaa
      541 ccatcacgtt tgaaattcaa aaagatatcg atttccacga aatcaacaaa gtccacgttc
      601 cggcactcct agactgccta agacaaacag aaaaaaggaa tcaataacat gatcttagaa
      661 accgtcatcc aaaaaatcga caaagacctt agagaaggaa tgaacgtcaa tgatctttct
      721 tctgaggata catcagctat cagagaggtc attcaattac ttgatcacgg caaactgcgg
      781 gtcgctgaaa aaattccggg cgccacgggt cctcaaagct ggaaggtaaa cgagtgggta
      841 aaaaaagcga tccttcagta tttccgaatt caaaaaatgc agttaatgga aacaaacgaa
      901 tttaactact tcgacaaaat tgctccaaaa aaatggactg gctctgaagg tgttcgcgtt
      961 gtgccaccgg cgattgctcg ctatggatcg ttcttaaact ccggctgcat aatgatgccg
     1021 tcatatgtaa acattggtgc gtatgtagac tcgggaacca tggtggacac gtgggctacg
     1081 gt
//
LOCUS       DAOMXO010000767         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_80273_length_1078_cov_5.188661, whole genome shotgun sequence.
ACCESSION   DAOMXO010000767 DAOMXO010000000
VERSION     DAOMXO010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80273_length_1078_cov_5.188661"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1078
                     /locus_tag="PLH57_12255"
     CDS             <1..>1078
                     /locus_tag="PLH57_12255"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK03426.1"
                     /translation="PDKKNLLNRRLQSPSERAKIRDYAHKLKETVFGQDEAIEMTAQA
                     MRAREMNPNIAQPVAMLFLGPTGNGKTELARAIAEVRYGNSEQMKKFDMGALKNADDL
                     KNFFGGTRGYMGSNTMSPFERFLNDFPEGGVVVFDEIGNMGRTPQEKQTMLEVFYPIL
                     DDGSWTSPLGKVYDELRKYTFIFTGNELQEFFNDMPNDDLREAVWRQTKSKPMIESAL
                     VKNHGWPPALIGRFENNLVLFRPVTSEVRRKVSEKLVNQALAGLRKAHNLGPIEFSQS
                     FYQKLGDTFFTHDKGARSIRGVVNNALVSLMGDAILESYEHGLPESARFDLELRDNFD
                     GRNFRSASAAPERTVTLAAKVISGS"
BASE COUNT          289 a          273 c          270 g          246 t
ORIGIN      
        1 ccccgataaa aagaaccttt taaaccgccg gttacaatcg ccgagtgaac gcgcaaaaat
       61 tcgtgactat gcccacaagt taaaagagac cgtattcggc caggacgaag ccatcgaaat
      121 gactgcccag gctatgcggg ctagggaaat gaacccgaac atcgcccagc cggtcgccat
      181 gcttttttta ggacccacgg gtaatggaaa aaccgaactt gcacgagcca ttgccgaagt
      241 tcgttacggc aattctgagc aaatgaagaa gttcgacatg ggggcactga aaaacgcgga
      301 cgaccttaag aatttttttg gtgggactcg cggttacatg ggttcaaaca caatgtcccc
      361 cttcgagcgg ttcttaaacg atttccctga aggcggcgtg gtcgtgttcg atgaaattgg
      421 aaacatgggt cgcactcccc aagaaaaaca aaccatgttg gaggtgtttt accccattct
      481 tgacgacggc tcctggacaa gccctcttgg caaagtttac gacgaacttc gcaagtacac
      541 cttcattttc actggaaacg agttgcaaga attctttaac gatatgccaa acgacgattt
      601 acgggaggcc gtctggcgcc aaacgaagtc gaaacccatg atcgaatcag ccctggtaaa
      661 aaaccacggc tggccgcctg cactcattgg gcgatttgaa aacaacctgg ttctttttcg
      721 gccggtcaca tctgaggtcc gacgaaaagt ttcagaaaaa ctggtgaacc aagcgctcgc
      781 cggacttcga aaggctcaca atttaggacc gattgagttt tctcagtcct tttatcaaaa
      841 actgggcgat acatttttta cccacgacaa gggagcgcgc tctattcgag gggtagtgaa
      901 caatgcgctc gtatctttga tgggggatgc gattcttgag tcctacgagc acgggcttcc
      961 cgaaagtgcc cgtttcgacc tggaacttcg agacaacttt gacggtcgta actttcgaag
     1021 cgcgagcgct gcccctgaac gcacggttac tctggctgcg aaagttataa gtggctcc
//
LOCUS       DAOMXO010000768         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_80274_length_1078_cov_4.860215, whole genome shotgun sequence.
ACCESSION   DAOMXO010000768 DAOMXO010000000
VERSION     DAOMXO010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80274_length_1078_cov_4.860215"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLH57_12260"
     CDS             <1..446
                     /locus_tag="PLH57_12260"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRK03427.1"
                     /translation="LIPLMITNALEGKPLPVYGKGLNVRDWIHVHDHCDGVLLACENG
                     KAGEVYNFGGRAEKKNIEVVQTILRLLKKPESLIRYVEDRKGHDWRYAIDCQKSEREL
                     NWKRKYSFESGLAETVSWYLENESWWREVKSGEYQNFYKNYYGEL"
     gene            454..>1078
                     /locus_tag="PLH57_12265"
     CDS             454..>1078
                     /locus_tag="PLH57_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRK03428.1"
                     /translation="MRGVILAGGTGSRLFPLTKVTNKHLLPVGRKPMIFHPVEKLVAA
                     GIDEILIVTGVEHMGDVVGLLGSGKDFGCRFTYRVQDEAGGIAQALGLAEHFVHGGLC
                     AVILGDNIFQDDLGPEVRRFEEQGKGARIILKQVPDPQRFGVAEISGEKVIGIEEKPK
                     NPKADWAVTGIYLYDSQVFDIVRTLKPSKRGELEITDVNNHYISQGQL"
BASE COUNT          316 a          199 c          285 g          278 t
ORIGIN      
        1 aacttattcc tttaatgatc accaatgcac tcgaagggaa accccttccc gtttacggaa
       61 aaggtttaaa cgttagggat tggattcacg ttcacgatca ttgtgatgga gtgctgcttg
      121 cttgtgaaaa tggaaaagca ggagaagtat acaatttcgg cggacgagcg gaaaaaaaga
      181 acatcgaagt cgtgcagaca attctacgat tattaaaaaa acccgaatcc ctaattcgtt
      241 acgtggaaga tcgaaaaggg catgattggc gttatgcgat agattgccaa aaatcagagc
      301 gagaattgaa ctggaagcgg aagtatagtt ttgagtccgg gctagctgag accgtcagct
      361 ggtacttgga gaacgaatca tggtggcgcg aagtgaagag cggcgaatac caaaattttt
      421 ataagaatta ttatggagag ttataaaatg aaagtgcgtg gcgtaatttt agccggtggt
      481 accggaagtc gactttttcc gctcaccaag gttaccaata aacatttact gcccgttggc
      541 cgcaaaccga tgatttttca tccggttgaa aaattagtag cggccggtat cgatgagatt
      601 cttatcgtca ctggggttga acacatgggc gacgtagtcg gactacttgg cagtggtaag
      661 gacttcggtt gtcggttcac ctatcgagtg caagacgaag caggtggaat tgcgcaggct
      721 cttggcttgg ccgagcattt tgttcatggt ggtctttgtg ccgtaatttt gggtgataat
      781 atttttcaag acgaccttgg ccccgaagtt cgccgatttg aagaacaggg taagggcgcc
      841 cgaattatat taaagcaggt acccgacccg cagcgttttg gagtagcgga aatcagcggt
      901 gaaaaagtta tcggtattga agagaaaccg aaaaatccga aagcggattg ggcggtgact
      961 ggtatttatc tttatgactc acaggtattc gatatcgtta gaacgctaaa gccgtcaaaa
     1021 cgtggcgaac tcgaaatcac tgatgtaaac aatcattata tttctcaggg gcaattaa
//
LOCUS       DAOMXO010000769         1069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_81294_length_1069_cov_3.245562, whole genome shotgun sequence.
ACCESSION   DAOMXO010000769 DAOMXO010000000
VERSION     DAOMXO010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81294_length_1069_cov_3.245562"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1012)
                     /locus_tag="PLH57_12270"
     CDS             complement(92..1012)
                     /locus_tag="PLH57_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03429.1"
                     /translation="MGARKQDIIYQELGELLFRTRVEKYRSALAMHREGKFSFSYAAY
                     ADFERGAALPSPAALLELCTFLTLDEKVAFATWARVQMPTEDLKALFSPETFLRDRRS
                     QQGRRDSEKNNPALVLATDPRMHQEEFENTWVFGIPERDAILKEPRLMEMFIHLSIIH
                     PRAVKLEDMGFRTSKDKQIFIERHLRRWIDEKRIEVTERGVRLMAPFIYMPRNEVFDQ
                     VRQTIVTRALEKTLPELTVHNINNRLAHRSTVNRYVSPKQREFMMEKLAGIEREFSRL
                     PYTKPDKSDETTKNHTLLIFCTPRKLEWPR"
BASE COUNT          246 a          236 c          268 g          319 t
ORIGIN      
        1 cctgttcgat gccgacttta agatttagtg agttggacat gttctccacc gcgatatttt
       61 agttctcgtt tttattgtcg catgcccgcc gctatctggg ccactcgagt tttcgaggtg
      121 tacaaaaaat caatagtgtg tgattttttg tggtttcatc ggacttgtcg ggtttggtgt
      181 aggggagccg cgaaaattcc cgttcaatac ccgcaagctt ttccatcata aattctcttt
      241 gcttgggtga tacgtagcga ttcacggtgg agcgatgtgc gaggcgattg ttgatattgt
      301 gcacggtgag ttcaggcaaa gtcttttcaa gcgctcgcgt gacgatcgtt tgacgaactt
      361 ggtcgaagac ttcgtttctt ggcatataaa taaatggtgc cattaaccgc accccgcgtt
      421 cggttacctc aattcgtttt tcatcgatcc aacggcgaag atgccgttct ataaatattt
      481 gtttatcttt tgatgtgcga aagcccatgt cttcgagttt aactgcgcgt gggtgaataa
      541 ttgaaagatg aatgaacatt tccatcaaac gaggttcctt gagaatcgca tcacgttctg
      601 ggattccgaa aacccaggta ttttcgaatt cctcttggtg catgcgtgga tcagtggcaa
      661 gcaccaaggc gggattgttc ttctccgagt ctctacggcc ctgttgactg cgtcgatcac
      721 gcagaaatgt ttccggcgaa aaaagcgctt ttaggtcttc tgtgggcatt tgcacgcggg
      781 cccaagtggc gaaagcgact ttctcatcta gagtgagaaa cgtacaaagt tctagtaacg
      841 ctgcaggcga ggggagggca gccccccgtt cgaaatctgc ataggccgca taggaaaaag
      901 aaaactttcc ttctcggtgc atcgcaaggg ccgagcgata cttttcgact cgagtacgaa
      961 aaagcagctc tcccagctct tgataaatga tgtcttgttt gcgggccccc atagtgacta
     1021 tttcgtcatt ttgacgaaaa ttttgagttt ttggctaaga ttttcgcat
//
LOCUS       DAOMXO010000770         1067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_81547_length_1067_cov_2.674901, whole genome shotgun sequence.
ACCESSION   DAOMXO010000770 DAOMXO010000000
VERSION     DAOMXO010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81547_length_1067_cov_2.674901"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..886
                     /gene="pstC"
                     /locus_tag="PLH57_12275"
     CDS             29..886
                     /gene="pstC"
                     /locus_tag="PLH57_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRK03430.1"
                     /translation="MLVLLLLAIAAFLFWNARETIQHFGFSFLTGTDWDPVQQNFGAL
                     PAVFGTVVSSLIRISIAAPLSIGVALFLTEVASGRLGRIVGFLVEMLAAVPSVVYGIW
                     GIFVLAPWLREKIQPALHGAFGFLPFFAKPFYGVGMFCAGVVLAIMIIPTIASIAREV
                     FAVVPRGQREAALALGATKWEMMRLSVLKASKGGLAGAMILGLGRALGETMAVAMVIG
                     NRLEISASIFSPSATMSSVIANEYAEAADRLHMAALVELGLILFFVTFVINAIARFFV
                     MSSSKRRWS"
BASE COUNT          220 a          234 c          296 g          317 t
ORIGIN      
        1 ttcgtggctt gtgtgggttc ccggagtgat gctggttttg ctactcttgg caattgcggc
       61 gtttctattt tggaatgccc gcgagacaat tcagcatttt ggcttttcat ttttaaccgg
      121 aaccgactgg gatcccgttc aacagaattt tggagcgctc ccagcagttt ttggaaccgt
      181 tgtatcgtcg ttgattagaa tatccattgc cgcacccttg agtattggag tcgcactttt
      241 tcttactgaa gttgcgtcgg ggcggctagg ccgaatagtt ggttttctcg tcgaaatgtt
      301 ggctgccgtt cccagtgtgg tttacggcat ttggggaata ttcgtgctcg cgccctggct
      361 gcgagagaaa atacaacccg ctctgcatgg ggcatttggt tttctacctt ttttcgcaaa
      421 acctttttac ggcgtcggaa tgttctgtgc gggtgtggtg ctcgcaatta tgattatccc
      481 gacgatcgct tcgatcgcac gcgaggtatt tgcagtggtt cctcgtgggc aacgtgaggc
      541 agcgttggct ctcggcgcga ccaaatggga aatgatgcgt ctgtcagtgt tgaaggcctc
      601 caagggaggg ttggctgggg ccatgattct ggggctcggc cgagcgctcg gagaaactat
      661 ggcggtcgca atggtaatcg gaaaccgact cgaaatctcg gcctcaatat tttcgccctc
      721 cgctactatg tcgagtgtga ttgcaaacga gtacgcagag gcggccgatc gtttgcacat
      781 ggcagcttta gttgaattag gactaatttt atttttcgta actttcgtaa tcaacgcgat
      841 cgcaaggttt tttgtcatga gttcgtcgaa acggcggtgg agttaagtat ggccatgaca
      901 gcttccgacc gggcaagaaa aaatagagcg ttacaaaaat ttatcgccaa gcggtatcaa
      961 cgacgaaaga ttgtcaatgg tttggcctat ctcgcaattg gtattgcgac gctaacggct
     1021 ttggttcctc tttttagtgt ctccgagttt attttaagaa aaggcct
//
LOCUS       DAOMXO010000771         1066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_81626_length_1066_cov_3.763600, whole genome shotgun sequence.
ACCESSION   DAOMXO010000771 DAOMXO010000000
VERSION     DAOMXO010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81626_length_1066_cov_3.763600"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1066
                     /locus_tag="PLH57_12280"
     CDS             <1..>1066
                     /locus_tag="PLH57_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03431.1"
                     /translation="LEAAAEAVEFSDGSSHYYWDVVSKEYQQYLGLLYRVVHQYGKKV
                     SLKEADIEKLFLEVSPNAAALLQRVVWDVANIGSFASYDITSYFEPSPNNPAKRRLVF
                     SPDAVIQHYGAALMAHPSEAEAKNALRREGIKILFAERASLLNFMYPNEKVERTIPEK
                     AEPWMRAKWEHSDQIRQESLVRDSMTIDWFQPITDHVRKGLKKLYTSPGIGPDLEVSR
                     GGLFFNSLATRYADKLSPKLLNDLLGDVDLVRTIKENGLSAEQGELLSTKVFVKTLVD
                     TYRSAFWKLVLASPYHLTLLSDRELENFLSETARMAHYQVLSEFVFAYFFERSGCKAG
                     PTRCLSEKEVGDIRTILAQWV"
BASE COUNT          298 a          249 c          249 g          270 t
ORIGIN      
        1 actcgaagcc gcggccgaag ccgtggagtt ctccgacggg agctcgcact attactggga
       61 tgtagtttcg aaagaatacc aacagtatct cgggctgctg taccgtgtcg tacaccagta
      121 cggaaaaaag gtgtcgctca aggaagccga tatcgaaaaa ttattcttag aagtatcacc
      181 caacgcggca gcacttttgc agcgagttgt ttgggacgtc gcaaacatcg gaagttttgc
      241 ttcttacgac ataactagtt atttcgaacc aagtcccaat aatccggcaa aacgccgact
      301 ggttttttcg cccgatgcag taattcaaca ctatggcgcg gcattgatgg cccacccttc
      361 tgaagctgaa gcaaaaaatg ctttgcgccg tgaaggtatt aagattctct ttgccgagcg
      421 agcgtcgctt ctcaatttca tgtaccccaa tgaaaaagtg gaacgcacca ttcccgaaaa
      481 agccgaacca tggatgcgcg caaaatggga acacagcgat caaattcgcc aagaatcgct
      541 agtacgcgat tcaatgacca tcgattggtt tcagcccatc actgatcatg tgcgaaaagg
      601 tctgaaaaaa ctttatacca gcccaggaat tgggcccgat ctcgaagtga gtagaggcgg
      661 tttattcttc aatagcttag ctacacgtta tgctgacaaa ctaagtccaa aacttctgaa
      721 cgatctccta ggtgatgtcg atctagtgcg cacgatcaaa gaaaatgggc tgagtgcaga
      781 acaaggagaa ttgctttcga ccaaagtttt cgtaaaaacc ttagtcgaca cctatcgaag
      841 cgcattctgg aaacttgtgt tagcgtcccc ttaccatctg acgcttttga gtgatcgtga
      901 gctagaaaac tttttaagtg aaactgcgcg tatggcccat taccaggtgc tttctgaatt
      961 tgttttcgct tactttttcg aacgatcagg ctgtaaagcc ggtccgacgc gttgtttaag
     1021 tgaaaaagaa gttggcgata ttcgtaccat actcgcgcaa tgggtt
//
LOCUS       DAOMXO010000772         1066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_81644_length_1066_cov_2.929773, whole genome shotgun sequence.
ACCESSION   DAOMXO010000772 DAOMXO010000000
VERSION     DAOMXO010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81644_length_1066_cov_2.929773"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..937
                     /locus_tag="PLH57_12285"
                     /pseudo
     CDS             47..937
                     /locus_tag="PLH57_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478132.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
     gene            927..>1066
                     /locus_tag="PLH57_12290"
     CDS             927..>1066
                     /locus_tag="PLH57_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03432.1"
                     /translation="MSANQKKKSGAASRILILDFGSQFTQLIARRLRELEVYCEIYPS
                     DAP"
BASE COUNT          254 a          243 c          334 g          235 t
ORIGIN      
        1 cccagcgcta tgactcgatg aaggcaaccg gcaagttgcc gtcgccaaag ggtgattgat
       61 cacaatcaaa gacatcctaa aacgagaaaa atacccatta gcagtgaaag atgggtttgg
      121 ccgtttaact gtgggagcgg ctgtcggagt tggggcggat ctcaaagagc gtgctgaaaa
      181 gttagtgagc gccggtgtcg acgtgctctt tgtagatagc gcgcatggac attccaagaa
      241 tgttttggat gcggtgaaag ggcttaagaa aaaatttaag ggcattgaag tggtgggcgg
      301 caacgtcgct actccggaag cagttcgggc actggtgcag gccggtgccg atgccgtgaa
      361 agtcggtatt gggcccggaa gtatttgcac gactcgcgtg gttgcgggca ttggcgtgcc
      421 gcaattttcc gccattcgtg agtgcgcgcg cgaagctgcg aaacaccgaa ttcccgtgat
      481 cggcgatggc ggaattaaat acagcggcga tgtagctaaa gcgctggccg gtggagcaga
      541 ggctgtaatg atcggctcac tttttgcggg cactgacgag tcgcccggtg aaaccatttt
      601 atatcaaggt cgcacctaca aagtttaccg tgggatgggt tccctgggtg ccatgcgtgt
      661 tggttcgaaa gatcgttatt ttcaagccga cgtcgatgaa gcgagcaaac tagtacctga
      721 aggaatcgaa ggtcgggttc cgcaccgcgg cacgctttcg agcaatatcc accaactcat
      781 cggtgggttg cgagcgggaa tggggtatgt tggcgcgcag ggccttgccg agcttcgcaa
      841 ggcgaaatac atccgaatta cggccgcggg gctcgccgag agtcacccgc acggggttgt
      901 gatcacgaag gaagcaccga attacaatgt cggctaatca gaaaaaaaaa tcaggtgcgg
      961 catcgcgtat tttgatttta gatttcggtt cgcagttcac gcagttgata gcgcggcgtt
     1021 tgcgcgaact cgaagtttat tgcgaaattt acccgagcga tgcgcc
//
LOCUS       DAOMXO010000773         1060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_82197_length_1060_cov_4.880597, whole genome shotgun sequence.
ACCESSION   DAOMXO010000773 DAOMXO010000000
VERSION     DAOMXO010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82197_length_1060_cov_4.880597"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1060)
                     /locus_tag="PLH57_12295"
     CDS             complement(<1..>1060)
                     /locus_tag="PLH57_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK03433.1"
                     /translation="NFFWSDYYARHLRQLGIEAFELFPNVPSLQKAWALENHAAGSNE
                     DLVLAQIKKINPDVIFLQASGAFSNAWVARLRESIPALRLLIGWRCSPFGANDIDNFK
                     MHDAIFTCNSSLTSAFEQMGVPSYVLHHAFEPSTFYSVSQMSESPWTRRLEKSLVFAG
                     SLVQAREFHLGRIGFIAGLLSAGIPLSLFAETDSFSRRLAKRLLGETESFLAKIRLSP
                     FLPARSFRRRASLWKDTQLWSPTHWAIAKNSYPALYGNEFYRVLGEAAVCLNFHIDSS
                     GPCAGNARLFEATGMGTCLLTDWKQNIGSFFSEDSEIVTFRNPTECIEKAVWLLENPT
                     AANDIGKRGQRRCFEHHSF"
BASE COUNT          312 a          240 c          254 g          254 t
ORIGIN      
        1 aaaagagtgg tgctcaaagc accgacgttg accgcgcttt ccaatgtcat tagcggcggt
       61 cggattttca agcaaccaaa ccgctttttc tatgcattca gtgggattgc gaaaggtcac
      121 aatttccgag tcctctgaaa agaacgaccc gatattctgt ttccagtcag ttagaagaca
      181 ggttcccatt ccggtggcct caaatagcct cgcattgcct gcgcatgggc cagaggagtc
      241 gatgtgaaag ttgagacaaa cggctgcttc accgagtaca cgataaaatt cgttaccata
      301 caatgcaggg tagctatttt tagctattgc ccagtgggtt ggcgaccata actgcgtgtc
      361 tttccagagg ctagcgcgcc gacgaaagga gcgagctgga agaaatggtg aaagtctaat
      421 tttggctaaa aacgattctg tttcgccgag taatcgttta gcaagtcgtc gcgaaaatga
      481 atcagtctca gcaaatagac ttaatggaat acctgcgctt aaaagacctg caataaaccc
      541 aattcttcct agatgaaatt cgcgggcctg aactagcgaa cctgcaaata ccagcgattt
      601 ctcgaggcgc ctggtccaag ggctttcgga catttgtgag acagagtaaa aagtgctggg
      661 ttcgaatgca tgatgaagta cgtaagaagg aacccccatt tgttcaaacg cggaagtaag
      721 cgaagaattg caggtaaata tggcgtcgtg cattttaaag ttatctatat cgttagctcc
      781 gaagggtgag caacgccatc caatgagcaa acgcaacgcg ggtatggact ctctaaggcg
      841 tgcaacccat gcattagaaa acgccccaga ggcctgaaga aaaataacat ctggattaat
      901 ttttttaatc tgcgccaaaa ctaaatcttc gtttgaaccg gcagcatgat tttctaatgc
      961 ccacgccttt tgcagtgacg gcacattcgg aaagagttca aacgcctcta ttcccagctg
     1021 gcgaaggtga cgagcatagt aatccgacca aaaaaaatta
//
LOCUS       DAOMXO010000774         1059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_82323_length_1059_cov_3.497012, whole genome shotgun sequence.
ACCESSION   DAOMXO010000774 DAOMXO010000000
VERSION     DAOMXO010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82323_length_1059_cov_3.497012"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLH57_12300"
     CDS             complement(<1..461)
                     /locus_tag="PLH57_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRK03434.1"
                     /translation="MSDHELESSNLPELSESERSTLEKALTRPDSVLPTTLFVVPVQS
                     APAFPGMFSPLVLSKPELVETIEEAMENRGLVGLLLSREATGSSGLDISGRPPKEPAK
                     TDSIEGIESLHSVGVAARIIRKLNDSTGGAAVLVHALKRFRVKKVVRNEPFL"
     gene            complement(509..>1059)
                     /locus_tag="PLH57_12305"
     CDS             complement(509..>1059)
                     /locus_tag="PLH57_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03435.1"
                     /translation="IQPGSNRVGFQTMKHAVKKWIRSSACLFALFTVSTFVACSSAPY
                     KTQKYASLSNTKVFEEDYEMVWKAILAAVSEIKLDEEDSESGRIRTDWIYSTSNDKYL
                     EYSVNGFPRKRYLQTRYKFLITAEKQLGGVKVTVIPEEEVERLKSDGSFESWVRVSEP
                     ETIRANEMVGQINQKILARPNL"
BASE COUNT          255 a          245 c          268 g          291 t
ORIGIN      
        1 agaaatggct cgtttcgaac cacttttttt actcgaaaac gtttgagcgc gtgaaccaag
       61 acggcggcgc cccccgttga gtcattgagt tttcgaatga ttctagctgc tacccccacg
      121 ctgtggagcg attcgatgcc ttcgatcgag tcagtcttgg cgggctcttt tgggggccgg
      181 cccgaaatgt ctaagcccga gcttccagta gcttcgcgac tcaacaatag gccgacaagg
      241 ccccggtttt ccatggcttc ttcaatagtt tcaactagtt ctggtttaga tagcaccagg
      301 ggcgaaaaca tacccggaaa cgccggtgcc gactgcaccg gcacgacaaa tagggtagtc
      361 gggagtactg aatccgggcg agttagagcc ttttccaggg tgcttcgctc agattccgac
      421 agttcaggta ggtttgaaga ttccaattca tgatcactca taccccctta aagatggggg
      481 caagagtgcg gcagtcaata cagctaagtt aaagattcgg acgggctagg attttttgat
      541 tgatttgacc gaccatttcg tttgctcgaa tggtttcggg ttcggaaact cgtacccagg
      601 attcaaagct gccatcggat ttaagacgct caacttcttc ttcgggtata acggttactt
      661 tgacaccgcc taattgtttt tccgcagtaa tgaggaactt gtaacgagtc tgtaaatacc
      721 gttttctagg aaatccattc acggagtatt cgaggtattt gtcatttgaa gtggaataaa
      781 tccaatcagt gcggattcgg ccgctttcgg agtcttcttc gtcgagttta atttccgaaa
      841 ccgcggcgag aatggctttc caaaccattt catagtcttc ttcgaaaacc ttggtgttcg
      901 agaggctggc gtatttttgc gtcttatacg gagcggacga gcatgcaaca aaagtgctga
      961 ccgtgaaaag ggcaaataaa caggctgaac ttcgaatcca tttttttacc gcgtgtttca
     1021 tggtttgaaa tccaacacga ttcgagccgg gttggatag
//
LOCUS       DAOMXO010000775         1058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_82452_length_1058_cov_3.163509, whole genome shotgun sequence.
ACCESSION   DAOMXO010000775 DAOMXO010000000
VERSION     DAOMXO010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82452_length_1058_cov_3.163509"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PLH57_12310"
     CDS             complement(<1..919)
                     /locus_tag="PLH57_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03436.1"
                     /translation="MEFIRLKKNKSREMSDFVANHLLFEYARNTLDPERKKAVESALE
                     STPELQNEFIKLINGIQYCEKLSETLINDAAIKQIELQSSQVNFIVNRLRWHQWPLGF
                     RWLVEAGVVALLLVVVLNILPLNRIIHLNDGNDSQTVVAEVDRKKKPNFAEAPEANIE
                     RPEFSDEEVKQPTTVAAKDPESAGKPTNTAPPVVPPKPVAATTPTVPTPAPKQATTVA
                     ATTPAVPEKTSSKSGAWIYRGQFLVTNAEAVSPKIKDKIKELGGQKAGEVEIGWLKTP
                     NVYYFHFTVPEAKLGELEALLKDYSTGKLV"
BASE COUNT          292 a          238 c          203 g          325 t
ORIGIN      
        1 taacaagctt acccgtcgag taatctttta ataaagcttc aagttcacct aacttagctt
       61 caggtacggt aaaatggaaa taatatacat ttggtgtttt caaccaaccg atttccactt
      121 ccccggcttt ttgaccaccc aattctttaa tcttatcttt gattttggga gagacagctt
      181 ccgcattagt tactaaaaac tgtccacgat aaatccaagc accagactta gatgacgtct
      241 tttcaggaac agcaggagtc gttgcagcca ccgtagtagc ctgttttggg gctggcgtcg
      301 gcaccgtagg agtagttgcc gctaccggtt tcggtggaac cacgggcggt gctgtattcg
      361 tcggcttacc tgcagattcg gggtctttcg cagcaactgt agtcggttgc ttaacttctt
      421 catctgaaaa ctctggtcgt tcaatattgg cctcgggagc ctctgcaaaa tttggtttct
      481 ttttgcgatc cacttcagca accacggttt ggctatcatt tccgtcattt aaatgaataa
      541 tgcgattcaa aggcaaaatg tttagaacga ctaccaacaa caaagcgaca acgcctgctt
      601 cgactaacca tcgaaaacca agtggccatt gatgccaacg caaacgatta acaataaagt
      661 ttacttgtga agactgcagt tcgatttgct taattgcggc atcattaatc agtgtttcac
      721 ttagcttttc acagtattgg ataccgttga tcaatttaat aaattcgttt tgtaattcag
      781 gggttgattc aagagccgac tcaacggctt tctttctttc agggtctaag gtattgcgag
      841 cgtattcaaa aagcagatga ttagcgacaa aatcactcat ctctctactt ttatttttct
      901 taagtctaat aaactccacg cctgccccta ataacttact ttaataaaaa cgtatttgat
      961 cctagttctt taagcgcact cgaaattctg tagcgatgag tcccttgaga aatatccagc
     1021 gcaaataaaa tatcgtcttc tggaacttta ataaccag
//
LOCUS       DAOMXO010000776         1050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_83382_length_1050_cov_2.758794, whole genome shotgun sequence.
ACCESSION   DAOMXO010000776 DAOMXO010000000
VERSION     DAOMXO010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83382_length_1050_cov_2.758794"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLH57_12315"
     CDS             <1..187
                     /locus_tag="PLH57_12315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRK03437.1"
                     /translation="LVQQGQQIAEVGATGKATAPHLHFEIRKGEMAQDPLLYLPQVRV
                     ASSGQNARRGPAVSSIE"
     gene            277..675
                     /locus_tag="PLH57_12320"
     CDS             277..675
                     /locus_tag="PLH57_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK03438.1"
                     /translation="MFDAKTRILVIDDMMTMRKVVAKCLREMGFSDILEAKDGADGWE
                     KITNAAPPVGLVISDWNMPNCMGIDLLKRVRADSRFKLTPFIMVTAESEQSQVKEALL
                     AGVTGYIVKPFTTELLKANLEKAYAKALGK"
     gene            682..>1050
                     /locus_tag="PLH57_12325"
     CDS             682..>1050
                     /locus_tag="PLH57_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03439.1"
                     /translation="MSNPSPLILAFGLEDIAKQYVAQNAPGAKIQDATAKDLDALMEN
                     PPNPPPVIIFLGINEKELPPVEAAQFFRAQFHETSIFFLSVNPTDQNRDALIKNGVND
                     VFVLTVDKDILKNSLQKGLSK"
BASE COUNT          278 a          237 c          267 g          268 t
ORIGIN      
        1 attggttcag caggggcaac agattgccga agtcggtgcc accggaaagg ccacggcccc
       61 tcatttgcat tttgaaattc gcaaaggtga aatggcccaa gatcccttgc tttatttgcc
      121 ccaagttcga gtggcgagca gtggtcagaa cgcgcgccgt gggccagccg tttcatcaat
      181 cgagtagtta aactcgtcgc ctattcaaga cgtggcgtgt tttcttcttg tcatctatca
      241 aaggcaatct aaacttaaac taaggagccg tgaaccatgt ttgacgccaa aaccagaatc
      301 ttagtgattg atgacatgat gacgatgcgc aaagttgtcg ccaagtgtct tcgtgaaatg
      361 ggctttagtg atattttgga ggcgaaagat ggcgccgatg gctgggaaaa gattaccaac
      421 gcagcgcctc cggtcgggct cgttatttcg gactggaaca tgcccaattg catgggcatc
      481 gatcttttga agcgggtgcg agcagattca cgttttaagc ttacaccttt cattatggtc
      541 acggccgagt cagagcagag tcaggtgaaa gaggccttgt tggccggcgt gactggctac
      601 attgtgaagc cgtttaccac cgaacttctt aaagcaaatt tggagaaggc gtacgcgaag
      661 gcgcttggca agtagggtgt aatgtcgaat cccagtcctc ttattttagc gtttggattg
      721 gaagacattg ctaaacaata tgtagcgcaa aatgctccag gcgccaaaat ccaagatgca
      781 actgctaaag acctcgatgc gctcatggag aatccgccga accctccgcc tgtaataatt
      841 tttcttggca tcaatgaaaa agaattgcca ccagttgagg cggcccaatt ttttcgcgcg
      901 cagtttcatg agacttccat tttttttctg agtgtaaacc cgacagatca aaaccgtgat
      961 gctttgataa aaaatggagt caatgacgtt ttcgtactca cggtcgataa ggatatactt
     1021 aaaaactcgc tgcaaaaggg gctgtccaag
//
LOCUS       DAOMXO010000777         1048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_83544_length_1048_cov_5.480363, whole genome shotgun sequence.
ACCESSION   DAOMXO010000777 DAOMXO010000000
VERSION     DAOMXO010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83544_length_1048_cov_5.480363"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1048
                     /locus_tag="PLH57_12330"
     CDS             15..>1048
                     /locus_tag="PLH57_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03440.1"
                     /translation="MLTTGEPPLFDDNGKVIKRSGEPGRHIGKRFKLSLSKEVEGAVR
                     ADMIGWKFKQLEGALLELSSTLAATIEKDLVSLTEYRPNVISSWQKYMSFDGLSYSSV
                     WFRPAANLIREFLLDLEQSYPVSPTNKKWADIAELKSLIESFDEVRDPYKMLGATFNE
                     ATNTLQLVERGAVVQYNEELATTELVPAKVEGMVKALQPVTHAMEGAGTNKSSGASYR
                     SLVGVYAGNGAILHAMDHTDAATGVMLGGEFFQKLTYTDRALQNTKQRFALPVNGITP
                     PLTLSGRSIGGTVALFYYLMFPDTVADQVVGISTSIPFTLPPQIANVFRQAEANSING
                     VVEGALDNTTA"
BASE COUNT          273 a          279 c          253 g          243 t
ORIGIN      
        1 ctcacggacg ggcgatgtta accactggtg agcccccact ttttgacgac aatggtaaag
       61 tcataaaacg ctcaggtgaa cccgggcggc acattggaaa acgatttaaa ctctcacttt
      121 caaaagaagt cgaaggcgcc gttcgagccg acatgatcgg ctggaaattt aaacagctcg
      181 agggcgcttt gttggaactt tcctccacac tcgccgccac catcgaaaaa gacttggttt
      241 ctctgaccga atatcggccc aacgtgattt caagctggca gaagtacatg agttttgacg
      301 gactcagtta ttcaagcgtt tggtttcgcc cagctgccaa cttgatacga gaatttcttc
      361 tcgacctcga gcaaagttac cccgtttcac ccaccaataa aaagtgggcc gacatcgcgg
      421 aattgaaaag tttaattgag agcttcgacg aagtgcgcga cccctataaa atgctcggtg
      481 ctacctttaa cgaagcgacc aacacgctgc aactcgtgga acgtggtgcg gtcgtgcaat
      541 acaacgaaga actcgccacc actgaattag tgcctgcgaa agtcgaaggc atggtgaagg
      601 ccctgcagcc cgttacccat gcaatggaag gcgctggcac caacaagtct agtggcgcca
      661 gttaccgatc gctcgtaggg gtttatgcag gcaatggcgc gattcttcac gccatggatc
      721 acacggacgc agccactgga gtgatgctag gtggagaatt ttttcaaaaa ctcacctaca
      781 ccgaccgcgc tctacagaac actaaacaac gtttcgcgtt accagttaac ggcatcaccc
      841 caccgctcac gctttcgggt cgaagtattg gtggtacggt tgctctcttt tattacttaa
      901 tgtttcccga cactgtagcc gaccaggttg tcggaatttc aacgtcgatt ccgtttactc
      961 ttccgccgca aatcgcaaac gtgtttcggc aagctgaagc taactctatt aatggggtcg
     1021 tagaaggggc actcgacaat acaactgc
//
LOCUS       DAOMXO010000778         1024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_86372_length_1024_cov_4.063983, whole genome shotgun sequence.
ACCESSION   DAOMXO010000778 DAOMXO010000000
VERSION     DAOMXO010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86372_length_1024_cov_4.063983"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PLH57_12335"
     CDS             <1..705
                     /locus_tag="PLH57_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRK03441.1"
                     /translation="DGVSGPATKFFKPNELENLKQACGLSTGDLLFVVADQERVANDA
                     LGSLRLQVGEKLGLIDKTKFNFLWVTDFPLLQFEPSDGRWYACHHPFTSPVMEDVAKL
                     ESGKDLGTIRAAAYDLVLNGVEVAGGSLRIFNQRTQAAMFRALGLSEKEAQEKFGFFI
                     EGLQYGTPPHGGIAFGMDRLTMILTGTDAIREVIAFPKTQKGACLMSESPSSITPEQL
                     AELHIGLRLPQKAEKH"
     gene            711..>1024
                     /locus_tag="PLH57_12340"
     CDS             711..>1024
                     /locus_tag="PLH57_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK03442.1"
                     /translation="MVYPEFSRATRVQKLAELSQSSAPVYDAVVIGGGIVGAGLLREL
                     AIRGVNALLVEKKDFASGTSSKSSKLVHGGLRYLEMFDFGVVLEALSERHWLLKTHPH
                     LVS"
BASE COUNT          248 a          237 c          279 g          260 t
ORIGIN      
        1 gacggtgtca gtggaccggc cacgaaattt tttaaaccaa acgaactaga aaatttgaaa
       61 caagcttgcg gcctatccac tggagatctt ttgttcgtgg tggccgatca ggaacgtgtg
      121 gccaacgacg ctttaggaag cctgcgtctt caagttggcg aaaagcttgg cctcattgat
      181 aagacgaagt tcaattttct ttgggtaacg gactttccat tacttcaatt cgaaccaagc
      241 gacgggcgtt ggtatgcttg ccatcacccc ttcacctcac cggtaatgga agatgtcgcg
      301 aaactcgagt cgggcaaaga tctcggcacc attcgtgctg ccgcctatga cttggtttta
      361 aatggcgtag aggtcgccgg tggtagtttg cggatattta atcagcgaac tcaggccgcg
      421 atgtttcggg cattgggatt gagtgaaaag gaagcccaag aaaaatttgg attctttatc
      481 gagggtctgc agtacggcac cccacctcac ggggggattg cttttggtat ggatcgatta
      541 acgatgattc ttaccggcac cgacgccata cgggaagtaa ttgcgtttcc gaaaactcaa
      601 aaaggtgcgt gcttgatgtc ggagagtccg tcgtcgatca cacccgaaca attggccgaa
      661 ctacacattg gtttgcgact gccgcaaaaa gctgagaaac actaaacttc atggtctacc
      721 ccgaattttc ccgcgccacg cgtgttcaaa agttggccga acttagccaa agttcagcgc
      781 cagtatacga cgccgtggtg attggcggtg gaatcgtggg agcgggtttg cttcgagagc
      841 tggccattcg tggcgtaaat gctttattgg tagaaaaaaa ggattttgcc agcgggacgt
      901 caagtaagtc ctcgaagttg gtgcatggag ggctgcgcta cctcgaaatg tttgatttcg
      961 gagtcgttct cgaagctctt tcagagcggc actggctttt gaagacccac cctcacttgg
     1021 tttc
//
LOCUS       DAOMXO010000779         1018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_87102_length_1018_cov_4.072690, whole genome shotgun sequence.
ACCESSION   DAOMXO010000779 DAOMXO010000000
VERSION     DAOMXO010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87102_length_1018_cov_4.072690"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1018)
                     /locus_tag="PLH57_12345"
     CDS             complement(<1..>1018)
                     /locus_tag="PLH57_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03443.1"
                     /translation="NILEGTVKLGFAGDANPMDLKIMGKDCRVSAAGSGSSVNISVDP
                     TNSKKQVSITGDGKIQLEADGKKSLIDPGKIAAVGENKKIEIEEEVFKQSLPRNNARL
                     VIGESTQIGDGRGNLPFEWSRRKTEVGTIEIDGAEHLQVPIQAEDTKTKVPLRSGDYR
                     WRLISRSQKSGSPWFDFSALVVDRPSIRYPAPQTAFRIPHTEDEKEIQLVFSHPNPDF
                     EVETQLYAVGKSGELVLKREDSLQPGARTGQQEVLELELEVGQYQLRARSVYTTKGQN
                     RKVYSPWTQPTTFAIIREPAPTPVPTPRPTPIARKPASEDMNSDSHLKKPAIRPRNGS
                     TIDEP"
BASE COUNT          211 a          248 c          255 g          304 t
ORIGIN      
        1 cggctcgtca attgtactcc cattccgcgg gcgaatggca ggttttttta agtgggaatc
       61 agagttcatg tcctcactcg cgggtttacg cgctattgga gttggacgtg gagtgggcac
      121 cggcgtaggc gcgggctctc gtatgatagc gaatgtcgtt ggttgcgtcc aagggctgta
      181 aactttgcgg ttttggccct tggtagtgta aacggagcgt gctcgcaact ggtactgacc
      241 gacttctagt tcgagctcta gaacttcttg ttgccctgta cgagctcccg gctgcaaaga
      301 gtcttcgcgt ttgagtacga gttctccaga cttaccaacc gcataaagct gagtttccac
      361 ttcaaaatct ggatttggat gcgagaatac cagttgtatt tctttttcgt cttcagtgtg
      421 tggaattcga aaagcggtct gaggggctgg gtagcgtatc gaaggtcgat caaccactaa
      481 cgcactgaag tcgaaccacg ggctgccgct tttctgtgaa cgtgaaatta aacgccaacg
      541 gtaatcgcca gaccgcagcg gaactttcgt tttggtgtct tcagcttgta tgggcacttg
      601 taggtgttcg gcgccatcaa tttctatggt accaacttct gtttttcggc gagaccactc
      661 gaaaggcaaa ttgcctcgac cgtctcctat ttgagtcgat tctccaatga ctaggcgagc
      721 gttgtttctt ggcagtgact gcttaaaaac ttcttcctca atttcgatct ttttattttc
      781 gcccacggcg gcaatttttc caggatcaat caaagacttt ttaccgtcgg cttccagttg
      841 aatttttcca tcgccggtaa tcgagacttg ttttttggaa ttcgtcgggt caacggaaat
      901 attcacgcta cttccgctgc ctgcagcact tactcggcag tccttaccca tgatcttaag
      961 atccattggg ttggcatcgc ccgcgaaacc taactttacg gttccttcca gaatgttg
//
LOCUS       DAOMXO010000780         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_87197_length_1017_cov_5.571726, whole genome shotgun sequence.
ACCESSION   DAOMXO010000780 DAOMXO010000000
VERSION     DAOMXO010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87197_length_1017_cov_5.571726"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1017)
                     /locus_tag="PLH57_12350"
     CDS             complement(<1..>1017)
                     /locus_tag="PLH57_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRK03444.1"
                     /translation="QSEPDASSFPSGGMRTTFEARGYTAWDPSSPVFIMENANGKTLC
                     IPSVFISYHGDALDEKAPLLRSMEVLNTKAVELLHVIGEKAVKRVVPTVGPEQEYFLI
                     DRAFYNLRTDLVMTGRTLLGGQPPRGQQLEDHYFGSISSRVLAFMQEVELELYKLGVP
                     AKTRHNEVAPSQYEMAPIFEEANVAADHNQLVMEVMRRVAARHNYAVLLHEKPFAGVN
                     GSGKHNNWSMATVGDDEQSPNLLDPGKTPHQNLRFLIVLSTVMKAVHRHAGLMRATIA
                     TPGNDHRLGANEAPPAIISVFLGEQLSEICNRIESGTNLEQSGEAAVISLGVSKIPQI
                     SKDNT"
BASE COUNT          247 a          262 c          244 g          264 t
ORIGIN      
        1 ggtattgtct ttcgaaattt gcggaatttt agaaacaccc aaactaatga ctgcggcttc
       61 cccgctttgt tcaagattag ttcccgactc tatgcgatta caaatttcgg acaactgctc
      121 tcccaaaaaa accgagataa tagccggcgg cgcctcgttt gctcccaggc gatgatcgtt
      181 gcccggagtc gctattgtgg cgcgcatcaa cccagcgtga cgatgaactg ctttcattac
      241 ggtagagagc acgattaaga atcgcaaatt ttggtgaggt gtcttacctg ggtcaagaag
      301 gttaggggac tgttcgtcat ctccaactgt ggccatcgac cagttgttat gcttgccgct
      361 gccgttcaca cccgcaaatg gcttctcgtg taacaacacc gcatagttgt gccgagcggc
      421 gacccgacgc attacttcca taacaagttg gttgtgatcg gccgctacgt ttgcttcttc
      481 aaaaattggc gccatttcgt actgacttgg cgctacttcg ttgtgacgcg ttttggccgg
      541 cacccctaac ttatagagct caagctcaac ttcttgcata aaagcaagaa cgcgcgatga
      601 aatgcttcca aagtagtgat cttcgagctg ctggcctcgc ggaggctgac cacccaataa
      661 agttcggccg gtcatcacaa gatctgttct caaattgtag aacgcccgat caatcaagaa
      721 atactcttgt tcgggaccca ccgtaggaac tacgcgtttg actgcctttt caccgataac
      781 atgcaatagt tcgaccgcct tagtgttcaa cacttccatg gaacgcaaaa gaggggcttt
      841 ttcgtctagg gcgtcaccat ggtaggaaat aaatacagaa ggaatacaaa gcgtttttcc
      901 gttagcattt tccattatga aaacgggact cgaggggtcc caggctgtgt atccgcgagc
      961 ttcgaacgtg gttcgcattc caccgctcgg aaaactagag gcgtctggtt cgctctg
//
LOCUS       DAOMXO010000781         1015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_87443_length_1015_cov_5.436458, whole genome shotgun sequence.
ACCESSION   DAOMXO010000781 DAOMXO010000000
VERSION     DAOMXO010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87443_length_1015_cov_5.436458"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLH57_12355"
     CDS             <1..273
                     /locus_tag="PLH57_12355"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400395.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRK03445.1"
                     /translation="MEIAKRLMDYGFHPPTVYFPLVVPGAIMIEPTETEPLHEIDRFI
                     SAMRAIAQEAKLNPEKVRTAPHTVFVKRVDETVAAKKLILRNNSTI"
     gene            287..565
                     /locus_tag="PLH57_12360"
     CDS             287..565
                     /locus_tag="PLH57_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03446.1"
                     /translation="MNFDHTARLFHTALVATKATKAANYSEQLTELVRTPAFVSILKS
                     VQNLAHELGITEEQAAERIIVAFRAIDSVWSEYLCKEGIESLKSHLSQ"
     gene            complement(567..>1015)
                     /locus_tag="PLH57_12365"
     CDS             complement(567..>1015)
                     /locus_tag="PLH57_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRK03447.1"
                     /translation="KLGMKPNLAPLGHLAPGSASSDYVRNAEQGAQTLLNTREYVYYS
                     YYQRVRQQLEQFWEPNLRSRLKKFFAKGRTLASDKEHSTRLMVHLDKEGTITKIEVRD
                     TSGLLELDQAAIDAFNKAGPFPNPPRGMLEANNGAVTVEWEFVLRT"
BASE COUNT          257 a          221 c          252 g          285 t
ORIGIN      
        1 atggagattg caaaaaggct gatggattat ggttttcatc caccaacagt ttattttcca
       61 ttagtggtgc ccggcgcaat catgattgag cccactgaaa ctgagcctct gcatgaaatc
      121 gatcgcttta tttctgctat gagggcgata gctcaagaag ccaaactcaa tccggaaaaa
      181 gtaagaactg ctccacatac cgtttttgtt aagcgggtcg atgagactgt ggcagcaaaa
      241 aaactcattc ttcgcaataa ttccacgatt tagcttttgg aaaatcatga attttgatca
      301 caccgcaagg cttttccata cggcgttagt tgctactaag gcgacaaagg cagcaaacta
      361 ttcagagcaa ctgactgaat tggttcggac gcctgctttc gtaagtattt tgaaaagtgt
      421 tcagaatctt gcccacgaat tgggcatcac ggaagaacag gcggcggaac gaattattgt
      481 cgcctttaga gcgatcgatt cggtgtggtc tgagtatctc tgcaaagagg gtatcgagtc
      541 tctaaaatct catttgtcac agtagatcag gtgcgtagta cgaattccca ttcgactgtg
      601 accgcgccat tgtttgcttc gagcatgcca cgtggtggat ttggaaacgg tcccgcctta
      661 ttgaacgcgt cgatcgctgc ttgatctaat tccagtaggc cggacgtatc tcttacttca
      721 attttagtaa tcgtaccttc tttatcgagg tgaaccatta ggcgagtgga gtgttctttg
      781 tcggaggcca aagttcggcc tttggcgaag aactttttaa ggcgcgacct gagattgggc
      841 tcccaaaact gttctaactg ctgacgaact cgttggtagt atgagtagta gacatattct
      901 cgggtgttaa gaagcgtttg cgcaccctgc tctgcatttc gtacatagtc actcgacgca
      961 gagcccggag caaggtggcc aaggggcgcg agattgggtt tcattccaag ttttt
//
LOCUS       DAOMXO010000782         1015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_87456_length_1015_cov_4.372917, whole genome shotgun sequence.
ACCESSION   DAOMXO010000782 DAOMXO010000000
VERSION     DAOMXO010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87456_length_1015_cov_4.372917"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..>1015
                     /locus_tag="PLH57_12370"
     CDS             386..>1015
                     /locus_tag="PLH57_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRK03448.1"
                     /translation="MTLLIVSLVAFLVSILTFFSGFGLGTVLTPVFAIWIPIDLAVAL
                     TAIVHFLNNLFKLVLVGRNANKQAVIRFGFPAIVAAALGAWVLVRVSNLSPITTYEMF
                     ERHYQIEPLKLTIALLIIAFTFFEIIPKLKNWTVAKKYISLGGLLSGFFGGLSGHQGA
                     LRSTFLIRMGLSKESFIATGVVIACFIDTVRLGIYWHHFSSDGISENATL"
BASE COUNT          236 a          231 c          214 g          334 t
ORIGIN      
        1 ggaaattcga tcttttactt cgtatactct gccgttcttt agcgtaatga agacttttga
       61 agaactcgag cttgccgatg ccggagcgcc caaaagcccc agaagcccca gaagcgccag
      121 acccgtgacc aatcttttaa caaataacac gaccattcct tctcatcggt ttttcggcgc
      181 ccacgaatta actcttcgca tgaaatcatt tatttctttc aataccatac aatataactc
      241 gggattatgc cgacctttct ggggcgctag tgcacttttg tctgggctta cccctaattc
      301 gaccggacac ttcaggtatg ataaaaggac tatcccatgg ttttcggatg aatcacattt
      361 tagaactctc ggagagcgtc gacgcatgac gttgttgatt gtttccctag tagctttttt
      421 ggtttcaatt ttaaccttct tttcgggctt tgggcttgga acggtgctca cgccagtatt
      481 tgctatctgg attccaattg atttggcagt ggctttgacg gccatcgttc attttcttaa
      541 taacctgttt aagcttgttt tggttggcag aaatgcaaat aaacaggccg taataaggtt
      601 tgggtttcct gctattgtag cagcggcgct aggagcatgg gttttagttc gcgtgtctaa
      661 tttatctccc attaccacct atgagatgtt tgaacgccac taccaaatag agccacttaa
      721 actcacgata gccctactta tcattgcttt cactttcttt gagattatcc caaagttaaa
      781 gaactggacg gtcgctaaaa aatatatctc tttgggcggt ctacttagtg gtttttttgg
      841 tggtctctcg ggtcatcaag gcgctcttcg gagcaccttt cttattcgca tgggcttatc
      901 taaggagagt tttattgcca caggcgttgt tatcgcatgt ttcatcgata cagttcgact
      961 tggtatatat tggcatcatt tctctagcga tggcatttct gaaaacgcga ctctt
//
LOCUS       DAOMXO010000783         1009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_88160_length_1009_cov_3.601677, whole genome shotgun sequence.
ACCESSION   DAOMXO010000783 DAOMXO010000000
VERSION     DAOMXO010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88160_length_1009_cov_3.601677"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLH57_12375"
     CDS             complement(<1..481)
                     /locus_tag="PLH57_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03449.1"
                     /translation="MGLRSKICILGLLVVFSSAAAYADEKRSNFSKIDKNSTLGWLKY
                     PTEPSPERRGEKKNEVIRKYQLPPKTKNNVYKAMLPAFYQDLDFDAYYRFTFEDLVWA
                     TLAIEGETDCDGREHSAAVLWTIAQRGGINPKRRLSNDKSYADALIEYCQPIKPKWAN
                     "
     gene            complement(529..>1009)
                     /locus_tag="PLH57_12380"
     CDS             complement(529..>1009)
                     /locus_tag="PLH57_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HRK03450.1"
                     /translation="YVETESFNPKYRNIKWENDEKLFKKWKSGETGFPLVDAGMRELV
                     QTGRMHNRVRMVVASFLVKDLHIDWRWGERFFMEHLLDGDLAANNGGWQWAASTGCDP
                     QPYFRIFNPQSQAKKFDPENSYIQKWVPEFGTKNELRPIVNHAERARRAVTLYRKLG"
BASE COUNT          242 a          268 c          215 g          284 t
ORIGIN      
        1 gattggccca tttcggctta attggctgac agtactctat caaggcgtcc gcgtaacttt
       61 tatcgttact caatcggcgt tttggattaa ttccgcctcg ctgcgcgatg gtccacaaca
      121 ccgcggctga atgttctcgc ccatcgcaat cggtctcacc ttcaatcgcc aaagtcgccc
      181 aaacaaggtc ctcaaaagta aatcggtagt aagcgtcaaa atcaaggtct tgatagaagg
      241 ctggtagcat tgccttgtag acattgtttt tcgttttggg cggcagttgg tattttcgaa
      301 tcacttcatt ctttttttcc cctcggcgtt cgggcgaggg ttcggtcggg tacttcagcc
      361 accccaacgt agaattttta tcgattttcg aaaaattcga tctcttttcg tcggcatacg
      421 ccgcagcact cgagaaaact acgagcaagc caaggataca aatttttgat cgcagaccca
      481 tcacgttatt ttcgcacgaa acaactcacg atttcaaaat cggcattttt agccgagttt
      541 gcgatacaag gtaaccgcac gccgcgcccg ttcagcgtga ttcacaattg gccgcaactc
      601 atttttggtg ccgaactccg gcacccactt ttggatatac gaattttcag gatcaaattt
      661 tttggcctgg gattggggat taaatatccg aaaatagggc tgaggatcac atcctgtcga
      721 cgccgcccac tgccagcctc cgttgttggc ggcaaggtca ccgtctaata aatgctccat
      781 aaaaaagcgt tcgccccacc gccagtcaat atgaagatct ttgactaaaa aactcgcaac
      841 caccatgcgc acgcgattgt gcattcgccc tgtttgcacg agttctcgca tgccggcatc
      901 caccagcgga aaccctgttt cgccgctttt ccacttttta aaaagttttt cgtcgttttc
      961 ccatttaata ttccgatact tggggttgaa actctcagtt tcaacatag
//
LOCUS       DAOMXO010000784         1001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_89188_length_1001_cov_4.731501, whole genome shotgun sequence.
ACCESSION   DAOMXO010000784 DAOMXO010000000
VERSION     DAOMXO010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89188_length_1001_cov_4.731501"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..532
                     /gene="rplS"
                     /locus_tag="PLH57_12385"
     CDS             149..532
                     /gene="rplS"
                     /locus_tag="PLH57_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRK03451.1"
                     /translation="MSQATKTQRTDKLALVEKKTIRTDITPFNPGDTVTVHVKIKEGD
                     KERIQLFEGVVIAFNHFGARKSFTVRKISHGVGVERVFPLHSPAVTKVEVVERGLVRR
                     AKIYYVRGRAGKAARIKTEASAHRS"
     gene            545..>1001
                     /locus_tag="PLH57_12390"
     CDS             545..>1001
                     /locus_tag="PLH57_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03452.1"
                     /translation="MTARNQPDFSTEFSYADALSIGVDEVGRGCIAGPVVAAAAVFPK
                     AILSEAAHRSDLFATKAKKPNLKTIQATLLPSSEEADPNVNLWGRPWWSWLDDSKKLS
                     PEMRAVLAQFVERFCRVGIGLADESEIDRLNILRATMVAIRRAVERVVRQ"
BASE COUNT          257 a          236 c          266 g          242 t
ORIGIN      
        1 taatcatctg tcggtacggg cggcggcggc cattgtttta gatcgggtct ttggtagaaa
       61 aagaaattag aatttgcgta tttgggcgtt tttaggttaa aagcgacctc taaattttat
      121 tactgaacta gtagttacgg gagccaccat gtcacaagcc accaaaacac aacgcacaga
      181 caaactcgcg ttggtagaaa agaaaactat tcgtacggat atcactccat tcaacccagg
      241 cgatacggtg accgtgcacg ttaaaattaa agaaggcgac aaagagcgta ttcagctttt
      301 cgaaggtgtt gtgatcgcgt tcaaccactt cggtgctcgt aagtctttca ccgtccgaaa
      361 aatttcccac ggcgtgggtg ttgagcgcgt gtttccgctt cactcaccag ctgtgacgaa
      421 agtcgaagtg gtcgagcgtg gtttggtacg ccgagcgaag atttactacg ttcgtggacg
      481 cgcaggtaaa gcagctcgta tcaaaactga agcttctgca cacagatcct aattagactc
      541 tcatttgact gcgcgaaatc agcctgactt ttcgacggag ttttcgtacg ccgatgccct
      601 gtcgatcggg gtcgacgaag tcggccgtgg ttgcattgct gggcccgtag tagcggccgc
      661 ggcagtgttt cctaaggcaa tattaagcga agccgcccac cgttcggatc tgtttgcgac
      721 caaagcaaaa aaaccaaatc tcaaaacaat tcaagcaacc ttgttgccga gctcggagga
      781 agcagaccct aatgtaaatc tctggggccg accctggtgg agttggttag acgactcgaa
      841 aaaactttct cccgaaatgc gagcagtgtt ggcgcaattc gtggagcgct tttgtcgagt
      901 cggaatcggc cttgcagacg agagcgagat tgatcgtcta aatattcttc gtgccacgat
      961 ggtcgccata cgtcgggcgg tagaacgagt ggtgcgacaa c
//
LOCUS       DAOMXO010000785         1001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Oligoflexia bacterium isolate AMR_MDS_5650
            NODE_89190_length_1001_cov_4.538055, whole genome shotgun sequence.
ACCESSION   DAOMXO010000785 DAOMXO010000000
VERSION     DAOMXO010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867871
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oligoflexia bacterium (wastewater metagenome)
  ORGANISM  Oligoflexia bacterium
            Bacteria; Bdellovibrionota; Oligoflexia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.51
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:59:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Oligoflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89190_length_1001_cov_4.538055"
                     /isolate="AMR_MDS_5650"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2599941"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLH57_12395"
     CDS             <1..458
                     /locus_tag="PLH57_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK03453.1"
                     /translation="VGIGICGLAGDICAEPKNLAKKLTELLRLSRNETKLIELAFDLY
                     TDSAKTSAHTAVERPSPERWVELATERRELIEFLKSFVRRARGPTEVQKDLALAFLEQ
                     ALRWARSPKKMTEWPNAQSVGSAAQGAALGKLLNQKHWETFWTRPKLDF"
     gene            526..>1001
                     /locus_tag="PLH57_12400"
     CDS             526..>1001
                     /locus_tag="PLH57_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK03454.1"
                     /translation="MLPRKARKLAKLKPAKAEPAKPRALESALRDDLMRMGFMHQSQG
                     DLEMAAEYYIRSLERSPTPEAHTFLGGVLRQMGELDQAIEECKMALEIDPNFGNALAD
                     LGSYLVEKRDYRRALPVLKKACRSKQYGSPAVPFFYLSRLYIRQGLLGAATECLQKA"
BASE COUNT          275 a          256 c          255 g          215 t
ORIGIN      
        1 tcgtaggaat cgggatttgc ggcctggccg gcgacatttg cgccgaaccc aaaaatctcg
       61 ccaaaaaact taccgaactt ttgcggctct cgcgaaacga aactaaactc atcgagcttg
      121 catttgatct ttatacagac tcagccaaaa caagtgctca cacggcggtg gagcgaccaa
      181 gccccgagcg ttgggtggaa ctggccacgg agcgccgcga gttaattgag tttttgaaat
      241 ccttcgtaag acgtgctcgc ggtccaactg aagttcaaaa agaccttgct ttagcgtttc
      301 tagaacaggc cttgcgatgg gccagatcac caaaaaaaat gaccgaatgg ccgaacgctc
      361 aaagtgtggg ttcggcggct caaggggcag cacttgggaa gcttttaaac caaaaacact
      421 gggaaacatt ctggacccgg cctaaacttg acttttaaac accgggcaag gcatatcaac
      481 taaatgagat cgaccagatc cgcacgtcgg gccacaaaga gggccgtgct acccagaaaa
      541 gccagaaaac tagcgaagct caagccagcg aaagccgagc ctgccaagcc acgtgctctt
      601 gaaagcgcgc ttcgagacga cctcatgcgc atgggcttta tgcaccagtc gcagggcgac
      661 ctagaaatgg ctgcagagta ttatatacgc tcgcttgagc gttcaccaac accagaggca
      721 catacttttt taggtggtgt attaaggcaa atgggtgaac ttgatcaggc aattgaagaa
      781 tgtaaaatgg ccctggagat cgatccgaac ttcggaaacg ccctcgctga ccttggttcg
      841 tatttggtcg aaaaacgaga ttatcggcgc gccctgccag ttttgaaaaa agcctgccgc
      901 tctaaacaat acggctcgcc cgcagtgcca tttttttatt tatcgcgcct ctatattcgg
      961 caagggcttt tgggagctgc taccgaatgt ttgcagaaag c
//
